LOCUS       DADLTF010000001       111714 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.001, whole genome shotgun sequence.
ACCESSION   DADLTF010000001 DADLTF010000000
VERSION     DADLTF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 111714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 111714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..111714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.001"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="JN749_000001"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="JN749_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HAY7366550.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="JN749_000002"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="JN749_000002"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HAY7366551.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="JN749_000003"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="JN749_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HAY7366552.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JN749_000004"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="JN749_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HAY7366553.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="JN749_000005"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="JN749_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JN749_000006"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="JN749_000006"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7366554.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JN749_000007"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="JN749_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HAY7366555.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="JN749_000008"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="JN749_000008"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HAY7366556.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="JN749_000009"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="JN749_000009"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HAY7366557.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="JN749_000010"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="JN749_000010"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HAY7366558.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="JN749_000011"
     CDS             12630..13529
                     /locus_tag="JN749_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7366559.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13548..13769
                     /locus_tag="JN749_000012"
     CDS             13548..13769
                     /locus_tag="JN749_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366560.1"
                     /translation="MTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQEGMHEQAIKIA
                     LRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13810..14280)
                     /locus_tag="JN749_000013"
     CDS             complement(13810..14280)
                     /locus_tag="JN749_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7366561.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14335..15537)
                     /locus_tag="JN749_000014"
     CDS             complement(14335..15537)
                     /locus_tag="JN749_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HAY7366562.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSSRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="JN749_000015"
     CDS             complement(15624..16166)
                     /locus_tag="JN749_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366563.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="JN749_000016"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="JN749_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HAY7366564.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /locus_tag="JN749_000017"
     CDS             complement(16909..17511)
                     /locus_tag="JN749_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAY7366565.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNMVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="JN749_000018"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="JN749_000018"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HAY7366566.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="JN749_000019"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="JN749_000019"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HAY7366567.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="JN749_000020"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="JN749_000020"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HAY7366568.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDFVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /locus_tag="JN749_000021"
     CDS             21909..22799
                     /locus_tag="JN749_000021"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HAY7366569.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24451
                     /gene="arnT"
                     /locus_tag="JN749_000022"
     CDS             22799..24451
                     /gene="arnT"
                     /locus_tag="JN749_000022"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HAY7366570.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDLWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24448..24783
                     /gene="arnE"
                     /locus_tag="JN749_000023"
     CDS             24448..24783
                     /gene="arnE"
                     /locus_tag="JN749_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HAY7366571.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24783..25169
                     /gene="arnF"
                     /locus_tag="JN749_000024"
     CDS             24783..25169
                     /gene="arnF"
                     /locus_tag="JN749_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HAY7366572.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25163..25435)
                     /gene="pmrD"
                     /locus_tag="JN749_000025"
     CDS             complement(25163..25435)
                     /gene="pmrD"
                     /locus_tag="JN749_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HAY7366573.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25540..26895)
                     /gene="menE"
                     /locus_tag="JN749_000026"
     CDS             complement(25540..26895)
                     /gene="menE"
                     /locus_tag="JN749_000026"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HAY7366574.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADVLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26892..27854)
                     /gene="menC"
                     /locus_tag="JN749_000027"
     CDS             complement(26892..27854)
                     /gene="menC"
                     /locus_tag="JN749_000027"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HAY7366575.1"
                     /translation="MRSAQVYRWQIPMDAGVLLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27854..28711)
                     /gene="menB"
                     /locus_tag="JN749_000028"
     CDS             complement(27854..28711)
                     /gene="menB"
                     /locus_tag="JN749_000028"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HAY7366576.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28726..29484)
                     /gene="menH"
                     /locus_tag="JN749_000029"
     CDS             complement(28726..29484)
                     /gene="menH"
                     /locus_tag="JN749_000029"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HAY7366577.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29481..31151)
                     /gene="menD"
                     /locus_tag="JN749_000030"
     CDS             complement(29481..31151)
                     /gene="menD"
                     /locus_tag="JN749_000030"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HAY7366578.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31240..32535)
                     /gene="menF"
                     /locus_tag="JN749_000031"
     CDS             complement(31240..32535)
                     /gene="menF"
                     /locus_tag="JN749_000031"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HAY7366579.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32614..32919)
                     /gene="elaB"
                     /locus_tag="JN749_000032"
     CDS             complement(32614..32919)
                     /gene="elaB"
                     /locus_tag="JN749_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HAY7366580.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32974..33435)
                     /locus_tag="JN749_000033"
     CDS             complement(32974..33435)
                     /locus_tag="JN749_000033"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7366581.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33500..34417
                     /gene="rbn"
                     /locus_tag="JN749_000034"
     CDS             33500..34417
                     /gene="rbn"
                     /locus_tag="JN749_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HAY7366582.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TAQRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34605..35826
                     /gene="elaD"
                     /locus_tag="JN749_000035"
                     /pseudo
     CDS             34605..35826
                     /gene="elaD"
                     /locus_tag="JN749_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            36369..36861
                     /locus_tag="JN749_000036"
                     /pseudo
     CDS             36369..36861
                     /locus_tag="JN749_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            36964..37467
                     /locus_tag="JN749_000037"
     CDS             36964..37467
                     /locus_tag="JN749_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HAY7366583.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(37534..38991)
                     /gene="nuoN"
                     /locus_tag="JN749_000038"
     CDS             complement(37534..38991)
                     /gene="nuoN"
                     /locus_tag="JN749_000038"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HAY7366584.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFDYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(38998..40527)
                     /gene="nuoM"
                     /locus_tag="JN749_000039"
     CDS             complement(38998..40527)
                     /gene="nuoM"
                     /locus_tag="JN749_000039"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HAY7366585.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(40691..42532)
                     /gene="nuoL"
                     /locus_tag="JN749_000040"
     CDS             complement(40691..42532)
                     /gene="nuoL"
                     /locus_tag="JN749_000040"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HAY7366586.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAAQV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(42529..42831)
                     /gene="nuoK"
                     /locus_tag="JN749_000041"
     CDS             complement(42529..42831)
                     /gene="nuoK"
                     /locus_tag="JN749_000041"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HAY7366587.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(42828..43382)
                     /gene="nuoJ"
                     /locus_tag="JN749_000042"
     CDS             complement(42828..43382)
                     /gene="nuoJ"
                     /locus_tag="JN749_000042"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HAY7366588.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(43394..43936)
                     /gene="nuoI"
                     /locus_tag="JN749_000043"
     CDS             complement(43394..43936)
                     /gene="nuoI"
                     /locus_tag="JN749_000043"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HAY7366589.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(43951..44928)
                     /gene="nuoH"
                     /locus_tag="JN749_000044"
     CDS             complement(43951..44928)
                     /gene="nuoH"
                     /locus_tag="JN749_000044"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HAY7366590.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(44925..47651)
                     /gene="nuoG"
                     /locus_tag="JN749_000045"
     CDS             complement(44925..47651)
                     /gene="nuoG"
                     /locus_tag="JN749_000045"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HAY7366591.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(47704..49041)
                     /gene="nuoF"
                     /locus_tag="JN749_000046"
     CDS             complement(47704..49041)
                     /gene="nuoF"
                     /locus_tag="JN749_000046"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HAY7366592.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(49038..49538)
                     /gene="nuoE"
                     /locus_tag="JN749_000047"
     CDS             complement(49038..49538)
                     /gene="nuoE"
                     /locus_tag="JN749_000047"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HAY7366593.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(49541..51343)
                     /gene="nuoC"
                     /locus_tag="JN749_000048"
     CDS             complement(49541..51343)
                     /gene="nuoC"
                     /locus_tag="JN749_000048"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HAY7366594.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(51437..52099)
                     /locus_tag="JN749_000049"
     CDS             complement(51437..52099)
                     /locus_tag="JN749_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HAY7366595.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(52115..52558)
                     /gene="nuoA"
                     /locus_tag="JN749_000050"
     CDS             complement(52115..52558)
                     /gene="nuoA"
                     /locus_tag="JN749_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HAY7366596.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(53189..54127)
                     /gene="lrhA"
                     /locus_tag="JN749_000051"
     CDS             complement(53189..54127)
                     /gene="lrhA"
                     /locus_tag="JN749_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HAY7366597.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            55071..56288
                     /locus_tag="JN749_000052"
     CDS             55071..56288
                     /locus_tag="JN749_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAY7366598.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            56372..56971
                     /gene="yfbR"
                     /locus_tag="JN749_000053"
     CDS             56372..56971
                     /gene="yfbR"
                     /locus_tag="JN749_000053"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7366599.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(57030..58862)
                     /locus_tag="JN749_000054"
     CDS             complement(57030..58862)
                     /locus_tag="JN749_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HAY7366600.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(58949..59599)
                     /gene="hxpA"
                     /locus_tag="JN749_000055"
     CDS             complement(58949..59599)
                     /gene="hxpA"
                     /locus_tag="JN749_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HAY7366601.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALSGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(59610..60104)
                     /locus_tag="JN749_000056"
     CDS             complement(59610..60104)
                     /locus_tag="JN749_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAY7366602.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(60187..60642)
                     /locus_tag="JN749_000057"
     CDS             complement(60187..60642)
                     /locus_tag="JN749_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HAY7366603.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            60980..62182
                     /gene="ackA"
                     /locus_tag="JN749_000058"
     CDS             60980..62182
                     /gene="ackA"
                     /locus_tag="JN749_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAY7366604.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            62257..64401
                     /gene="pta"
                     /locus_tag="JN749_000059"
     CDS             62257..64401
                     /gene="pta"
                     /locus_tag="JN749_000059"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAY7366605.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            64591..66111
                     /gene="yfcC"
                     /locus_tag="JN749_000060"
     CDS             64591..66111
                     /gene="yfcC"
                     /locus_tag="JN749_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAY7366606.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(66144..66686)
                     /gene="yfcD"
                     /locus_tag="JN749_000061"
     CDS             complement(66144..66686)
                     /gene="yfcD"
                     /locus_tag="JN749_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HAY7366607.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(66744..67295)
                     /gene="yfcE"
                     /locus_tag="JN749_000062"
     CDS             complement(66744..67295)
                     /gene="yfcE"
                     /locus_tag="JN749_000062"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HAY7366608.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(67351..67995)
                     /gene="yfcF"
                     /locus_tag="JN749_000063"
     CDS             complement(67351..67995)
                     /gene="yfcF"
                     /locus_tag="JN749_000063"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HAY7366609.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            68131..68778
                     /gene="yfcG"
                     /locus_tag="JN749_000064"
     CDS             68131..68778
                     /gene="yfcG"
                     /locus_tag="JN749_000064"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HAY7366610.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            68835..69197
                     /gene="folX"
                     /locus_tag="JN749_000065"
     CDS             68835..69197
                     /gene="folX"
                     /locus_tag="JN749_000065"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HAY7366611.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            69218..70111
                     /locus_tag="JN749_000066"
     CDS             69218..70111
                     /locus_tag="JN749_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HAY7366612.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(70159..71049)
                     /locus_tag="JN749_000067"
     CDS             complement(70159..71049)
                     /locus_tag="JN749_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HAY7366613.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(71246..72019)
                     /gene="hisP"
                     /locus_tag="JN749_000068"
     CDS             complement(71246..72019)
                     /gene="hisP"
                     /locus_tag="JN749_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HAY7366614.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEACERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(72027..72743)
                     /locus_tag="JN749_000069"
     CDS             complement(72027..72743)
                     /locus_tag="JN749_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7366615.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(72740..73426)
                     /locus_tag="JN749_000070"
     CDS             complement(72740..73426)
                     /locus_tag="JN749_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708190.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7366616.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(73516..74298)
                     /gene="hisJ"
                     /locus_tag="JN749_000071"
     CDS             complement(73516..74298)
                     /gene="hisJ"
                     /locus_tag="JN749_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HAY7366617.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(74519..75301)
                     /gene="argT"
                     /locus_tag="JN749_000072"
     CDS             complement(74519..75301)
                     /gene="argT"
                     /locus_tag="JN749_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HAY7366618.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(75567..76136)
                     /locus_tag="JN749_000073"
     CDS             complement(75567..76136)
                     /locus_tag="JN749_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7366619.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(76231..77748)
                     /gene="purF"
                     /locus_tag="JN749_000074"
     CDS             complement(76231..77748)
                     /gene="purF"
                     /locus_tag="JN749_000074"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAY7366620.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(77785..78273)
                     /gene="cvpA"
                     /locus_tag="JN749_000075"
     CDS             complement(77785..78273)
                     /gene="cvpA"
                     /locus_tag="JN749_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HAY7366621.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(78532..79194)
                     /gene="dedD"
                     /locus_tag="JN749_000076"
     CDS             complement(78532..79194)
                     /gene="dedD"
                     /locus_tag="JN749_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HAY7366622.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(79184..80452)
                     /gene="folC"
                     /locus_tag="JN749_000077"
     CDS             complement(79184..80452)
                     /gene="folC"
                     /locus_tag="JN749_000077"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAY7366623.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(80522..81436)
                     /gene="accD"
                     /locus_tag="JN749_000078"
     CDS             complement(80522..81436)
                     /gene="accD"
                     /locus_tag="JN749_000078"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HAY7366624.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(81592..82251)
                     /locus_tag="JN749_000079"
     CDS             complement(81592..82251)
                     /locus_tag="JN749_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7366625.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(82334..83146)
                     /gene="truA"
                     /locus_tag="JN749_000080"
     CDS             complement(82334..83146)
                     /gene="truA"
                     /locus_tag="JN749_000080"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAY7366626.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(83146..84159)
                     /locus_tag="JN749_000081"
     CDS             complement(83146..84159)
                     /locus_tag="JN749_000081"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7366627.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(84225..85361)
                     /gene="pdxB"
                     /locus_tag="JN749_000082"
     CDS             complement(84225..85361)
                     /gene="pdxB"
                     /locus_tag="JN749_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HAY7366628.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            85460..86455
                     /locus_tag="JN749_000083"
     CDS             85460..86455
                     /locus_tag="JN749_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HAY7366629.1"
                     /translation="MIQPISGPPLGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(86452..87630)
                     /locus_tag="JN749_000084"
     CDS             complement(86452..87630)
                     /locus_tag="JN749_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7366630.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLCFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(87905..89125)
                     /gene="fabB"
                     /locus_tag="JN749_000085"
     CDS             complement(87905..89125)
                     /gene="fabB"
                     /locus_tag="JN749_000085"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HAY7366631.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            89284..91290
                     /gene="mnmC"
                     /locus_tag="JN749_000086"
     CDS             89284..91290
                     /gene="mnmC"
                     /locus_tag="JN749_000086"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HAY7366632.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(91411..91689)
                     /locus_tag="JN749_000087"
     CDS             complement(91411..91689)
                     /locus_tag="JN749_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HAY7366633.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(91723..92271)
                     /locus_tag="JN749_000088"
     CDS             complement(91723..92271)
                     /locus_tag="JN749_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HAY7366634.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(92271..93080)
                     /locus_tag="JN749_000089"
     CDS             complement(92271..93080)
                     /locus_tag="JN749_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HAY7366635.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(93080..93904)
                     /gene="mepA"
                     /locus_tag="JN749_000090"
     CDS             complement(93080..93904)
                     /gene="mepA"
                     /locus_tag="JN749_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HAY7366636.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(93908..94993)
                     /gene="aroC"
                     /locus_tag="JN749_000091"
     CDS             complement(93908..94993)
                     /gene="aroC"
                     /locus_tag="JN749_000091"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAY7366637.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(95028..95960)
                     /gene="prmB"
                     /locus_tag="JN749_000092"
     CDS             complement(95028..95960)
                     /gene="prmB"
                     /locus_tag="JN749_000092"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7366638.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            96126..96677
                     /gene="smrB"
                     /locus_tag="JN749_000093"
     CDS             96126..96677
                     /gene="smrB"
                     /locus_tag="JN749_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HAY7366639.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(96781..97671)
                     /locus_tag="JN749_000094"
     CDS             complement(96781..97671)
                     /locus_tag="JN749_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HAY7366640.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(97736..98182)
                     /locus_tag="JN749_000095"
     CDS             complement(97736..98182)
                     /locus_tag="JN749_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7366641.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNT"
     gene            complement(98179..98649)
                     /locus_tag="JN749_000096"
     CDS             complement(98179..98649)
                     /locus_tag="JN749_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7366642.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(98646..99194)
                     /locus_tag="JN749_000097"
     CDS             complement(98646..99194)
                     /locus_tag="JN749_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7366643.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(99199..99921)
                     /locus_tag="JN749_000098"
     CDS             complement(99199..99921)
                     /locus_tag="JN749_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7366644.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPYQYKV
                     TLTRNGQEFTVNNPTSYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSC"
     gene            complement(99941..102582)
                     /gene="yfcU"
                     /locus_tag="JN749_000099"
                     /pseudo
     CDS             complement(99941..102582)
                     /gene="yfcU"
                     /locus_tag="JN749_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(102664..103227)
                     /locus_tag="JN749_000100"
     CDS             complement(102664..103227)
                     /locus_tag="JN749_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7366645.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            complement(103911..104396)
                     /gene="sixA"
                     /locus_tag="JN749_000101"
     CDS             complement(103911..104396)
                     /gene="sixA"
                     /locus_tag="JN749_000101"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HAY7366646.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(104599..106743)
                     /gene="fadJ"
                     /locus_tag="JN749_000102"
     CDS             complement(104599..106743)
                     /gene="fadJ"
                     /locus_tag="JN749_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HAY7366647.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(106743..108053)
                     /gene="fadI"
                     /locus_tag="JN749_000103"
     CDS             complement(106743..108053)
                     /gene="fadI"
                     /locus_tag="JN749_000103"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HAY7366648.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(108233..108517)
                     /locus_tag="JN749_000104"
     CDS             complement(108233..108517)
                     /locus_tag="JN749_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HAY7366649.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            108889..110229
                     /gene="fadL"
                     /locus_tag="JN749_000105"
     CDS             108889..110229
                     /gene="fadL"
                     /locus_tag="JN749_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HAY7366650.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            110595..111653
                     /locus_tag="JN749_000106"
     CDS             110595..111653
                     /locus_tag="JN749_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366651.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNCQAK
                     YQNEINQLSALGVNFDDI"
BASE COUNT        27998 a        29874 c        27464 g        26378 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa ggtatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gcgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgagcaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgct ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgatcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgttgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcggc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa tgaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgccgatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attgcccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gggataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcaggaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggctgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcagg ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata accatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatggttc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tttcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtttatt gtctatgcca tcggcactta tgccgtgctc gatccgttta ttgcattctg
    23221 gctggtggcg ggaatgtgca gcttctggct ggcaatgcag gcacagacgt ggaaaggcaa
    23281 aagcgcagga tttttactgc tgggaatcac ctgcggcatg ggggtgatga ccaaaggttt
    23341 tctcgccctt gccgtgccgg tattaagcgt gctgccatgg gtagcgacgc aaaaacgctg
    23401 gaaagatctc tttatttacg gctggctggc ggttatcagt tgcgtactga cggttctccc
    23461 ctggggactg gcgatagcgc agcgggagcc tgacttgtgg cactattttt tctgggttga
    23521 gcatattcaa cgctttgcac tagatgatgc ccaacataga gctccgttct ggtactacgt
    23581 gccggtcatc attgccggta gcctgccgtg gctgggatta ctccccggtg cactgtacac
    23641 aggctggaaa aaccgcaagc attccgcaac cgtctatttg ttgagctgga cgataatgcc
    23701 gctgctgttt ttctccgtcg ctaaaggcaa attgcccacc tatattcttt cttgctttgc
    23761 acctctggca atgctgttgg cgcattacgc tttgctggca gcaaaaaata atcctctggc
    23821 gctgcggatt aatggctgga ttaacatcgc ttttggcgtc actggcatta ttgccacatt
    23881 tgtgatctcc ccctggggac caatgaacac gccggtgtgg caaaccttcg agagctataa
    23941 agtcttttgt gcctggtcga ttttttcgct atgggcattt ttcggctggt acaccttaac
    24001 aaacgtcgaa aagacctggt ctttcgccgc gctttgcccg ctggggctgg cgttgctggt
    24061 aggattttca attcctgaca gagtcatgga aggaaaacat ccgcaatttt ttgtcgagat
    24121 gacacaagaa tcacttcagc caagccgcta tattcttact gatagcgtcg gtgttgccgc
    24181 aggtctggca tggagcctgc aacgcgatga catcatcatg tatcgccaga caggtgagtt
    24241 gaaatacggc cttaattatc cggatgcgaa agggagattt gtcagcggtg atgagttcgc
    24301 aaactggctt aatcaacatc gtcaggaggg gattattaca ctcgtgcttt cggttgaccg
    24361 cgatgaagat atcaacagtc tcgccattcc gcccgcagat gccatcgttc gtcaggagcg
    24421 tctggtgctg attcagtatc gtcccaaatg atctggctaa cattagtctt tgccagcttg
    24481 cttagcgttg ccgggcagtt gtgtcagaaa caggcaacct gctttgtggc gataaacaaa
    24541 cggcgcaaac atatcgtgct gtggctggga ctggcgctgg cttgtcttgg tcttgccatg
    24601 gtgctctggc tgctggtctt gcagaacgta ccggtaggca ttgcctaccc gatgttaagt
    24661 ctgaattttg tctgggtgac gctggctgca gtaaaactgt ggcacgaacc ggtatcgccg
    24721 cgtcactggt gtggggtggc gttcattatt ggtggcattg tgatcctcgg gagtacggtg
    24781 taatgggcct gatatgggga ttattcagcg tcattattgc ctcagttgcg caattaagcc
    24841 tgggttttgc ggcaagtcat ctgccgccga tgacgcactt atgggatttt atcgcggctc
    24901 tgttagcttt tggcctggat gcccggatac tgttactcgg gctgctgggt tatctgcttt
    24961 ccgttttttg ttggtataaa acgctacata aacttgcgct aagcaaagcc tatgccttgc
    25021 tcagtatgag ttatgtgctg gtatggattg cttcaatggt acttcccggc tgggaaggga
    25081 ctttttcgct gaaagcacta ctgggagtag cctgtattat gagcgggttg atgctgattt
    25141 tcctgcccac gacaaaacaa cgttactgag ttttccctgc cgctgtacac tgccgttccc
    25201 actcatcggg gctataactg cttgccgaga ggactttcac cgtatgcagc ttttcacgat
    25261 taatacaata aagcgcattc tgcaaaggcg agagtaaatc ccccactttc acggcgaagt
    25321 cactcttcac ttcggcgatc atttttatcg cgccgccagc atcgcagagc ataagtacat
    25381 gtctgttatc ttgtttattg cagcacgatt ttttgtgcca gccattccat tgcattatcc
    25441 tgtttgctaa gagttttcac atcaatttac agattaacag ataaactctt tatcaacggc
    25501 aatggaatgg cagaaatgag aagtgaaacg gagggttaat tattgctgac gttgcaccca
    25561 ctcttttagc gcctgacgtg aaattttaat accgccgttt ttcagctccg gcggtaaagt
    25621 cagccagcgc accggttgtt gaaaacgagc cagcttatct ttcacccatt cgccaagatc
    25681 aacggtttgc tggtcatact ccaccaccgc taccggccga tgaccaaact ccttgtcggc
    25741 aacggaaacg ataaacacct gcaaaaccgc aggatgtgca gcaataacac gctcgacttc
    25801 ttccggctga ataccctcac cgccactgaa gaacagattg tctaaacgtc cgacaatggt
    25861 cagcttgcca ttatgcatct caccgcgatc gcgcgtagcg taccagcctt cgtcattaac
    25921 cagtgaaacc agttgcccgt tacgccagta accttctgcc atactggcag cccgcagcca
    25981 cacttcatta ttaacgattt tcacttcccg acccggcagc ggcaaaccaa cgtctgccag
    26041 gacgtcggct tctttcgcac ataccgtgga ggcaaactcg gtcagaccat agccgcaaaa
    26101 gcaacgaatc ccctgctcgc gcgcctgttc cgtcaactcg accgggatag ccgcgccgcc
    26161 aagtaacacc gctttcaggg aaacggaact gcggttaacc agcaaacgcc agagttgtgt
    26221 tggcaccagt gaagcgtgag tacagcctgc cagcatttgc tccaatggct gtttatcacg
    26281 caccgtcatc cgcgcaccag cgtataacca gcgccacata attccctgac cggagacgtg
    26341 aaacagcggt aaagagagca accagtcatc gtgatcgcca aacggaatca gcgataacac
    26401 accttgcgca ctggcaagat gggcctgata agtatggaca gcggctttcg gcaaaccggt
    26461 agaacccgag gtcaacgtca ttgagcacag acgcgtcggc tgccacgcag cggcatgtac
    26521 gccttcaacc agctgaatgt gcagcgacgt taatgccgga aacgtgtttt ccccatccgg
    26581 caccagcgca aattgcagcg tcagattggg cagcaattct tcaagcaacg gttgcggcag
    26641 ctgagggttc acgggcaaca cccgcgcccc gcattgcagt aacgccagcc aggcgagcag
    26701 cgtttgcggc gtattccacg cccgcaacat cacgccgctg ccctcaacca caccctgcgc
    26761 cgcaaagccc gtagctaact catcgacgcg agcacaaagc tcgcgccagt tgagttgctc
    26821 gtcattaaga cgtaaggcga tggcttctcc ccgcacttgc cgccagtgac gccacggcca
    26881 gtcagagaag atcataacaa ctgctccagt acatcagcgt ccactaacgg cagtgggctc
    26941 cccggccagc gacgaacctg ctgcgcctgc atcagatcca gcgtgtccag tcccggaatg
    27001 gtgtctggcg ttaaccaggc agcaatccgc gccagttgcg ttagacctaa gctcgactca
    27061 atggaagaac tgatcaccgc cgtcagcccc agcgcatgcg ccgcctgtac ctgctcgcgt
    27121 actttttcca gactgcccgt gagcgtgggt ttgataacta ccgcgcgcac gccctcttca
    27181 gccacaaagg caaaatccgg ctcgcgcagg ctttcatccc aggcaatggc aatgccggtt
    27241 tcacgggcaa acgctcgcga atcatcgcgg gttttgcacg gctcttcgag aaacgcgatg
    27301 cggtggcgat aatccgggtt aacatatttg gcaaactgct gacctttcag cggtgtccag
    27361 gcgcgatttg cgtcaagacg caaatgcaga tccggaattg cctccagcaa cagattcacc
    27421 accatgccgt cgcgcaccgc ttcgtacaat cccactttga ccttcgccac tttctcgccc
    27481 ggcatatcgg caagtttgag gatcagatcg tccggatcgc cattacacag cggtgccgca
    27541 cggtagttgg ctgcttgcgg caacgtatct gtcagttctg ccaatgcaca gcttacgcca
    27601 aaggccacgg aaggcatctg cggtagctcg caatcgcctg ccagccagtt atttacccag
    27661 gcaagcagca cgctttgcgc ctcttcccag ttttcctggc tgaagcccgg cagtggggag
    27721 atctcccccc acccttcgcg ctcgccctca cgcaggcaaa cgtacagccc gtcgcgggtt
    27781 tttaaccgcc tgtcgcgcag aagcaccccc gcgtccatgg ggatctgcca gcggtatacc
    27841 tgcgcgctac gcattacgga ttccgtttaa atttgctgaa atcaggctga cgtttctggt
    27901 tgaaagcgtt gcgaccttcc tgaccttctt ccgtcatgta gaacagcatg gtggcgttgc
    27961 ccgccagctc ctgcagcccc gcctgcccgt cacagtcggc gttcagcgca gctttcaggc
    28021 agcgcagcgc catcgggctg ttttgcagca tttcgcggca ccaacggacg gtttcttttt
    28081 ccagatccgc cagcggtacc acggtgttga caaggcccat atccagcgcc tgttttgcgt
    28141 cgtactgacg gcacaggaac cagatttctc gcgctttttt ctgcccgacg atacgagcca
    28201 tgtaggaagc gccccagccg ccgtcgaagg aaccgacttt cgggccagtc tgaccgaaga
    28261 tggcattatc tgctgcgata gtcaggtcgc acatcatgtg cagaacgtga ccaccgccga
    28321 tggagtagcc agccaccatc gcgacaaccg gtttcggaca ggtacggatc tgacgctgga
    28381 agtccagcac gttcaggtga tgtacgccgg aatcatcttt atagccgccg taatcaccac
    28441 gcactttctg gtcaccaccg gagcagaacg ctttatcgcc tgcgccagtc agaatgatca
    28501 cgccgatatt gtcgtcataa cgcgcatctg ccagtgcctg gatcatctct tttaccgtca
    28561 gaggacggaa ggcattgcgc acctgcggac gattaatggt gatttttgcg ataccgtcgg
    28621 tggatttttc ataacgaatg tcctcgaaac cttcggagca gtcgtgccat tcaaccggtg
    28681 cgtaaggcat tgcttcatca ggataaatca tattgtgtcc tttagtcaaa aacgcaagat
    28741 ctgcgccaga cttgcgatta cgccagcggg attttcccga tgcgcgttat gtccggcgcg
    28801 aggaatgaca tggcagtcgg cagccagttc cgccgctagg gcgcggaatt tgctgtcacg
    28861 ttcaccacat aaataataaa acgcaaatgt gcgggcgcta aggttagcac gtaaatcagg
    28921 ctggacggcg agagaagtcg cctccagcat ggcggcaagg gttgcgccat tattgttgct
    28981 gcgcagcgcc accagctccc ggcgttgatc gtcattgagc gaggcaaaaa caggctgttg
    29041 ataccagtcg gcaaataccg ccgttaacgg ttctgtgcga aaacgctgcg cccattggcg
    29101 atcggaacgc tgacgttccg cacgttgttc agcattttgc agccccggat gcccgccttc
    29161 gataataacc ccacaaagcc ccgccagccc ctggcaagcc gccatcatcg ccacccgtcc
    29221 gccaagcgag taccccacca gccagaagtc aaggatgttg taactaacca aggttttacg
    29281 cagtaagtcg gtgacatcat caaatccatc gacgctaatc gccgccgaac caccatgacc
    29341 tgggagatca acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt cttgccattc
    29401 gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag ggtaaacctg gttttccgtg
    29461 ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg ccagaagttg ctggagcgtt
    29521 tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg cgtgcgccag
    29581 gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg gacgatgata tttcagctcg
    29641 aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca tcagatagaa acgctcacgc
    29701 tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt caccacaatt
    29761 aataccagcg gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc gtaaagtgcg
    29821 gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac gcctgcggcg
    29881 gtcgaaagca gtccgtcgat accgctggcc cctcggttgc tgtacaccgg gtaacctgcc
    29941 ggaagttgcg aaagcgcatc aatcagacgt accaccaggc tgttaccaac aaacaattgc
    30001 ccctgttcag gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc aaacgcatca
    30061 cggcgggcaa taaccgcctg cattgcctgt tccgccaggc gcgggatttc aacgcaccag
    30121 ggctggcgtt tttctgccgg atgcagctcc agccagtcgg caatattgcc aattaagcga
    30181 cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat ccagtactct
    30241 tctggttcac agcttgcctg ccattgcagg agccgtttcc ccgtcaggct gcttcccagt
    30301 tgcaccacaa tttgtgcctg ctgcagctcg ctggtcgctt tggcattgcc taaccaaaga
    30361 tcggcacacg gcagcggctg cccggtttgt gacagcacat cgccaatcag cggccagcca
    30421 agagtttgcg cccacagggc aacttttttg ccctcttccg cactcatgcg cccggcaacc
    30481 accacgccgc gcttttgtcg ccagaagaac cagtcgcgct gtttttcact ttccagacga
    30541 ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt cacccagacg ctgttgccag
    30601 ctaagcccgg tatcgtccat ttcgccatac agcggttcag caaacgggca gttgatatgg
    30661 actccccccg catgaagcgt accgagagcg tggtcgatgg tagaaaccag ccaacgtgcg
    30721 gggatatcct gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga ggcgaacatt
    30781 cccggctggc gaattgcctg attcgcgccg cagtcaatta gctccggcgg gcgatcggcg
    30841 gttaagagaa tcagtttttc tccggttaac ccggcttcaa tcagtgccgg atagagattt
    30901 gccaccgccg tgccggaggt cacaatcacc gccaccggct gcttgctgac tttcgccagc
    30961 cccagcgcca gatgccccaa cccacgctca tcgaaatggg tgtggtgaat gaatgcggaa
    31021 ttctccgccg ccgctaacgt taacggtgta gaacgcgagc ctggggcgat acagatgtgt
    31081 ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg gttaaatgcg
    31141 cttactgaca tgagtttgtc cggtatcaat attgcggcta agtataagga gctacaaaaa
    31201 tagattattg atatgaatcg gtaatgatgc gactcattac tattccattt gtaataaagt
    31261 acgcagccct gccgctttgt tgtcgatttc ctgccactct tgctcggggt cggaaccacg
    31321 gacaatgccc gcgccagcat ataatcgcac gacattgccg ctaatttttg ctgagcgcag
    31381 ggaaacgcag aattcgcttt gttgtaatga gagatagccc gcagaaccgg cgtaccattc
    31441 tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg gtaagccagc
    31501 aactgctgcc gttggctgca actgatgtaa acagatcaca tcatccgctt tgttgagtga
    31561 agtccagata cagcggcgaa gatgctgcac tttacgcaga cgcagtacct gcggcggtaa
    31621 aacatccagc gtctgggtat cggcctgtaa tcgttggcag atatcttcca ccaccagcat
    31681 gttctcgcgc tggtttttat catccgccat cagccactct cctaactgct gcgcctgctt
    31741 atcatcagga tgatttgcta ctgttcccgc cagcgcttca gtacgcagcg ctttgtcacg
    31801 ccgccgccat aaccgttccg gtgaagagcc aagaaaagca ttttcgccat caaaggccat
    31861 gtaaaaatgg tagcaattca gattcagtcg acgactggca gccatcatcg ccgccgcgtt
    31921 gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc accactttgt cgagctcacc
    31981 ttcggcgatg gttttcgttg ccagttcgat taattgcgtc cagcccgttt tgtccggcca
    32041 gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac tcaccagtgt
    32101 ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc tgaatagcgt
    32161 caaccgcagc gtggctttac cgccacagcg tcgccattcc aggcggggta aaagtaaatt
    32221 gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt aagtcggcgt gttccgggtg
    32281 ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg cgcccaggac
    32341 ggcagcttct tcatcaccat tacgttgttg ccagtagaat tgcgggtacg tttgctgact
    32401 ggccagccag ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat cgataacccg
    32461 aatgccgggt gtcgccggaa tctcttgcga caaatggcgc agtagatttt ccagcgccgt
    32521 agtaagtgat tgcacgcgga cctctccctg ttaaaagcct cacattatac ggggtactac
    32581 aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg cgtgccagca acagtcctag
    32641 taccagcccg acggccgcgc ccacaccaat tccttgccag ggtttttcgt ggacgtagtc
    32701 atcagcacga taaacagcct gcttcgcccg atagtaataa ctgtctgaag cctggctaac
    32761 ccgttttttc acatcatcca gcgctttttc tgcacgcgct ttcagctcaa catatttctg
    32821 atcggcgggc tcgccagagg agcggagcac ctcttccagt gtttcactaa gcagcgtcag
    32881 gtcgtcatcg atacgtgtat caccaaactg attagacatt ctcgttctcc atttgcgtaa
    32941 aacctgtcac taactataga caagagttgt tgattacgcc tgaattacct cgcgtgccat
    33001 cccaatgtgt gggataccat cttcttcata gacctccgtc acagggataa aaccgaaact
    33061 ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat caggccagtg
    33121 atgcgtacat gtttcgagtg ttttactcat cagctgctgc cccacttttt caccacgcaa
    33181 cgcttcactg acaatcaccc gacctataac gacaggctca agatcatcat cacttttcag
    33241 aatcctcgca tacgccacca gttcatcatt tttccaaccg agaatatggc gattatcccc
    33301 cgtcaaatca tcaccatcga tatcctgata agggcaattc tgctcgacaa caaacaccgc
    33361 gcaacgcagt tgtaataagg cataaagctg ggaaaccgat aattcagagt gatgcagatc
    33421 ttgccattca atcataatgc tctcctttta gatgtatgcc ttatactagc cggaatattt
    33481 tttgaaacgt gatgaactca tggaattaat ttttttaggt acttcagccg gtgtgccaac
    33541 ccgcacgcgc aatgtcacgg caatattgct taacctgcaa catccgaccc agagcggact
    33601 ttggttgttt gactgcggtg aaggcaccca gcatcagcta ctgcataccg cctttaaccc
    33661 tggaaaactg gacaagattt ttatcagtca ccttcatggc gatcatcttt ttggtttacc
    33721 cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa cccttaacga tttatggtcc
    33781 gcaaggtatc cgtgaatttg tggaaaccgc gcagcggatt agcggctcat ggaccgatta
    33841 tccgctggaa attgtcgaaa ttggcgctgg cgaaattctc gatgatggcc tgcgcaaagt
    33901 aaccgcttat ccgctggaac acccactgga atgttatggc tatcgtattg aagaacatga
    33961 taaaccgggt gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc ctggcccact
    34021 gtttcaggaa ttaaaagcgg gcaaaacaat cacgctggaa gatggaaggc agattaacgg
    34081 cgcagattac ttagctgctc cagtgccagg taaagcgctc gctattttcg gcgataccgg
    34141 cccctgcgat gccgcacttg acctggctaa aggtgtcgat gtcatggtgc acgaagcgac
    34201 gctggatata accatggaag ccaaagccaa tagtcgcggc catagctcta cacgccaggc
    34261 tgcgacacta gcccgtgagg ctggagtcgg caagctaatc attacccacg tcagctcgcg
    34321 ctatgatgac aaaggttgtc aacacctgtt acgtgaatgc aggtcaattt tcccggcgac
    34381 tgaactggcg aatgatttca ccgtgtttaa cgtttaactc tgcttattat taaaaacagg
    34441 gtgaaacttg ccctgttata gcaacccgcg ctacatatat ccacccacac aggcatattg
    34501 agcgattttt gatatttatt catctcctta cgcactatat tcagagcaat aagttgcctc
    34561 ttttcatctt gattcagcac aacctttgca actaaaagga gcccatgatg gttacagttg
    34621 tcagcaatta ttgtcaatta tctcaaaccc aactcagtca gacatttgca gaaaaattta
    34681 ctgtgaccga ggaattactg cagtctttaa aaaaaacagc gttatccgga gatgaagaaa
    34741 gtattgagtt actgcataat attgcgttag gttatgatga atttggaaaa aaagctgaag
    34801 atattcttta ccatattgtt agaaacccaa caaatgagac cctatcgatt atccgactta
    34861 ttaaaaatgc ctgtttaaaa ttatataatt tggcacatac cgcaaccaac tcccacctta
    34921 aaccaactgg tccggataat tcagatgttc tcctgttcaa aaagctattc tccccttcga
    34981 aattaatgac aattatcggt aatgaaattc ctcttatatc tgaaaaacag tcgctttcaa
    35041 aggtgctttt aaatgatgag aataatgaac tgagtgatgg tacaaacttc tgggataaaa
    35101 atcgtcaatt aaccacagat gaaatagctt gctatcttca gaagatcgcc gctaatgcaa
    35161 aaaatactca agtcaattat cctactggtc tctacgtccc ctactccacc agaactcacc
    35221 tggaagacgc tctcaatgaa aatattaaga gcgatccatc atggccgaat gaagtccagt
    35281 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
    35341 aaaaaaaata ataaactaca aataaaatgc gtcatattca actcattgcg tgcactaggc
    35401 catgataaag aaaattcact taagcgtgtc attaatagtt ttaattctga actcatggga
    35461 aaaatgtcga ataataatat aaaagttcat ttaaatgaac cagagataat atttttacat
    35521 gccgatcttc agcaatactt aagccaaagt tgcggtgcgt ttgtgtgcat ggcagcccag
    35581 gaagtgattg aacaaaggga aagcaattct gacagcgccc cctatacgtt attaaaaaac
    35641 tatgctgaca gatttaaaaa atattcagca gaagagcagt acgaaattga ttttcaacat
    35701 cgactggtaa acagaaattg ttatttagat aaatatggcg atgcacgaat caatgcttct
    35761 tatacacaat tagaaataaa acactcacaa cccaaaaata gagcatccgg caaaagagtg
    35821 agttaacatc attgttaatc aattcgacca gtcagtaaat atgctgactg gtcgaacata
    35881 atttgctaaa aagtcatcac tgactgatgt cagtcccacc atctgccagt tcaatctacg
    35941 ggtatttcca gcgtatcgcg gcacgcgatt gctggtctgt tgatgtagtg gattgtagtt
    36001 gctgactttt cttaccctca gatttctgcc ccacttgtca attcaatctg ccaataaata
    36061 acataattat atttttattc ctcacagaaa ttccttttta aaaatagcga acccactgta
    36121 aaaaacactt tttgtatacc tctaccaagc gtaataaaat aaacgctcat aattcattat
    36181 aaataataat gaaataacgc cttttattga taatttacat atgaaaaaaa ttttcctttt
    36241 gttatattat ttctgttttc actccctctg attattttta ccaaccctgg gtcactgcat
    36301 tatcaccaac gccccgacac gtaagtcctg agcaattaga aaaaacagtt cgttatctta
    36361 cacaaactgt gcatccacgt aatgccggca atattgataa tctgaatagg tccgcagagt
    36421 atataaaaga gttctttgtc agtagcggtg ccagagttac cgcgcagggc gtccccgatt
    36481 ggctattacg actctgcgcc tggaagccag aattaccctt acccggcaat gtcctggctt
    36541 tatcccgatc agggagactt tattgctgtg gtcggcagaa tgcaggatat caacgccgtt
    36601 cgtcaggtaa aagcggcatt gttgtcatcc cgggatttat ctgtttattc tatgaatgcc
    36661 ccagggttta ttcccggtat tgatttttct gatcacctta attattggca acacgatatt
    36721 cccgccgtaa tgattactga caccgctttt tatcgtaata aacaatacca cttgcccggt
    36781 gataccgcag acagattgaa ttatcagaaa atggctcagg tggtagatgg tgttataacc
    36841 ttgttataca acagtaaata ataaaaattt ctcgaggcgg gtccagaaac agcgacatgt
    36901 acattttatt ttccctcgtc tgttttaaga tgcgaaacat aaaattcaat ggaggaaaaa
    36961 taaatgggaa tgattggcta ttttgcagaa attgactcag aaaagattaa ccaactgctt
    37021 gagtctactg aaaaaccttt gatggataat atacatgaca cgctttcagg attacgccga
    37081 ctggatattg ataaacgctg ggatttttta cactttgggc tgacaggcac ctctgctttt
    37141 gatcccgcga agaatgatcc gctgagtcgt gccgtgcttg atgaacacag cctcgaagat
    37201 ggcatagatg gcttcctggg attgacctgg aatcaggaat tagccgcgac aatcgaccga
    37261 ctggaatcac tggatcgaag tgagctgcgt aaacagtttt ccattaagcg actcaatgaa
    37321 atggagattt atccgggagt gaccttttca gaagagttag aggggcaact ctttgcctcg
    37381 atcatgcttg atatggaaaa gctgattagc gcgtatcgac gtatgctgcg acaagggaat
    37441 catgcgctga cggttattgt tgggtgatgg tgtcagagcg ccaaaaaaaa ccgccgaaca
    37501 tgtcggcggc tttctgactt acaaactaac agtttacatc agcggcattg ctaaccgcac
    37561 aatactaatc agcggttgtg gccatacacc cagcaccagt accaacagtg cagaaatcag
    37621 caccacgata ccgcccgcgc tgtactgcca gtttgacggt gcatcgcgac ccggttgttc
    37681 cggtgcgtgc aggtacaggc tcaccgccac gcgcaggtag tagtagaggc cgattgccga
    37741 accgacaacc acggcaccca ccagccacca caagtgtgcc tggacaccga ccgccagcac
    37801 gtagaactta ccgataaagc ccagcgtcat cgggataccg gccagagaca gcatcatcac
    37861 cgtcatcact gccgcgagga tcggacgatg ccagaacaga ccgcggtaag agaacaggga
    37921 atcagcatcc gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag
    37981 gctgctgaac agataaccgg ccaggtaaac ccctaccgct tccatcgaca tctcgccggt
    38041 ttgcagcgca atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag
    38101 cagacgtttg atgttggtct ggctcaacgc catcaggtta ccgaagatga tggaggcaaa
    38161 ggcgataatc gccagtacta cgcgaatcgc ttcgctgtca cccaccggtg cgtagaggaa
    38221 cagacgcatc accacaccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac
    38281 cggcgcaggc gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag
    38341 tttgaagccg aggccaacaa tcatcatgcc gaaacctgcc agcaacagcg gctcgttgag
    38401 cataccgtcg ccgaggtttt tacccagcgc gacaaacgac aggtcgccag actgcgcata
    38461 caccagcgcc ataccgaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt
    38521 gatactggct tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg
    38581 caaagagatc agttcaatac cgaggaacag agacgccaga tggtttgcat tcgccagcag
    38641 gatcccgccc agcgcggcaa ttaacaccaa caggtagaac tcatccttgt tgtcgttata
    38701 gccttcaagc cacgggtagt cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc
    38761 ggtgtaaagc atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcccgc
    38821 ctggccaaca aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc
    38881 gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc
    38941 gacgatcagc aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta
    39001 cggccttgta gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg
    39061 cgaggtatcc agaatcggct gcggatagaa gcccagcagt accagcagca ccaccagcaa
    39121 caggatcata aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctggct
    39181 tttcgcttta ccgaagtaag cgcgatgcaa catcgccagc gaataaacag atgcaaagac
    39241 cagcccaaag gtagagataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat
    39301 cataaattcg ccgacgaagt taccggtgcc aggcatccca agcgttgcca ccgcaaagaa
    39361 cagcgacagt gctggcagcc atttcatctt gctccacaga ccgcccatca tgcgcatgtc
    39421 gcgggtatgg atacgttcat aaagctgacc gcagagaata aacagacccg ccgccgacaa
    39481 gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat
    39541 tgcaatcagc acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc
    39601 ggtctgagcg aaggccatcc acgcaccgta gaagatgccg ataacaccca gccacatagc
    39661 gattggcgcg aactctgccg acgcgttcgg gaacagcggc agggagaaac gcaacaaacc
    39721 gtaagcggcg gttttcagca agatccccgc gaggtccacg gaaccggcgg tcggagcctg
    39781 ggagtgcgca tccggcagcc agccatgcag cggaaccacc ggcattttga ctgcgaaggc
    39841 gatgaagaag cccagcatca acagatattc cacaccactg gacattggcg tattcagcag
    39901 ctcttcatag ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa ccagcgccag
    39961 gatggcgatc aacatcacca gaccactcgc ctgggtgtaa atgaagaact tggttgccac
    40021 cgtgatacgc gttttaccgt cagaggcttt atgcccccac agtgcgatca ggaagtacat
    40081 cggcaccagc atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac
    40141 gccgataacg ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc
    40201 gatctctttc cacgaacata gtaccgccag cacaccgagc agaccggtca gcacgaccat
    40261 cagcagcgac agaccgtcaa tggcgagatg aatagagata ccaaaacgcg ggatccacgg
    40321 catgtcgaat tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc
    40381 ctgcaaccac agttgcagcg acagcgccag cgtcaatccc atggtgatca gcgcgatcca
    40441 gcgtggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc caataaaggg
    40501 aattaatatt agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag
    40561 gaccggattt tcaacgaatt ctcacgacag aaaattctct tcggactggg ggtagtgtcg
    40621 gatgcgccgc gaaggcagct tatccgacct acgttcttat atgcccaggg gcaatcccac
    40681 aatccttaac tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca
    40741 tggatgccac ataccagcgc agataaccgt tctcgcttaa cagcagacct ttacctgcaa
    40801 agcgggaaag aaccgccggg atgttcatca ttgagttcag cggatcgcgt ttcagcaacc
    40861 aggcaatacc caggaacggc ttgacgaaca ctttgtcgta cagccagtca aagccccagg
    40921 cgttgtacca ccaggtactc agcaggcggc ccggcgcact gttggcgatg gaggtcacca
    40981 gagtacgttt acccagccac agccaggctg ccagcagaat gccgaccacc gcgaccacgc
    41041 cagaggtaat ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca
    41101 cgccctgcag cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa
    41161 tcagcggcag gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt
    41221 ggaagacgat gaaaatcata cggaaggtgt agagcgaggt cataaacgca ccgaccagac
    41281 ctgccaccat cagattgata tgaccattcg ccatcgcacc cgcgaggatc tcatccttac
    41341 tgaagaagcc cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc accaggaagc
    41401 agagataaac cagcggaata gatttacgca gaccgcccat cttgaagatg ttctgttcgt
    41461 gatggcaggc cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt
    41521 gagtcatcaa gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt
    41581 agccaatctg gctcatggta gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg
    41641 cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat acccaccaga tgcagaactt
    41701 ccggcgtcat caggaacagg ccgtgggtac gggcgatcag gtagacaccc gcggttacca
    41761 tcgttgcggc gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg tcagcaagcc
    41821 atgtctgcaa cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg
    41881 cccacatcag catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatttcgc
    41941 ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt
    42001 cacccacacg ggtcacgacg aacgctttca ttgctgctgc gccattcttc ggatcggtgt
    42061 aatagaaacc gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca
    42121 gcagcaggtt atcggcgaga accagaacta ccatgctggc gatgaacagg ttggtgtagg
    42181 cgaagaagcg agagtagccc tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa
    42241 ggaaacccac accagtgacc accgagagca tggtcagcga caggccgtcc agcaccaggt
    42301 taaaaccgat gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgcct
    42361 gctcgccgtt agcgaagaaa tcaacgccga taaaggcggt tacctgcgcc gccaggccca
    42421 cagagccaac gccgacgatc gccgaaacgt tttcagacca gcgcccacgg gagaacgcca
    42481 gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccgcgcat
    42541 ctcacttact gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc
    42601 aaggccgata ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc
    42661 gtcggtctga ccccagtagc ttccggcgac cacaaaggcc agcgcggagg cgttaatcat
    42721 gatttccaga ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc
    42781 aagaacgaat aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc
    42841 tccgtttttc ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct
    42901 tcacgaccga cgtggaaggc cacaaccaga cccgcgagca gcagcataga agccagttcc
    42961 accgccagta cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta
    43021 ccgtcgatac cctgatcgtt aacaccgagg atggcgtaaa caatcaccac cagcatgatg
    43081 gccgacaaaa ttgccgggcc aatccacacc tgcggtttca gccactggcg ttcctgttcg
    43141 atttctgaac cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca
    43201 cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag cgcctagtga gaagaacacc
    43261 ccggaaatcg ccagcagcga aataatcagg tacagcagtg cgtgtaccgg attggtatgg
    43321 gtgatcactc gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc
    43381 attgcccctc tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg
    43441 cttcgccctt atctttgccg tcgattgcca tacctgccat ccggtagaag ttatattccg
    43501 ggtatttgcc cggaccggag atcagcagat cctctttctc gtaaaccaga tcctggcgct
    43561 tgtattcccc catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt
    43621 cgcacagacc acagaaaatg cagcgtgaga agttaatgcg gaaaaattcc ggataccagc
    43681 gaccgtcttt ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcac
    43741 agaggttaca ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac
    43801 cacgataacg gggcggcaga tagaccggct cttccgggta cattcgcgtt tcgcgtttgg
    43861 cgaacgcgtg caggccgatc atccagatac tacgaacctg ggtgccgaaa cctactaaca
    43921 attcttttaa ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc
    43981 cgttaccagc aagttgatca gcgtcagcgg caggcagatt ttccagccga aggacattac
    44041 ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa
    44101 cgcggttttc agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa
    44161 gaacagcgtc accatcaatg cagagatggt cacaatcccg atgtactcac ccacgaagaa
    44221 cagaccgaac ttcataccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc
    44281 ttccggctgg tcaaacgggt gacggtgaca caccgccacg cccgcgatgg caaaggtaat
    44341 aaaaccaaag aattgcggga tgacgttcca cacatgcgcc tggctgttga cgatgtcggt
    44401 catgttgaat gaaccggcct gcgccaccac gcccatcagg gaaagcccga ggaacacttc
    44461 gtagctcagg gtctgcgcag aagcacgcat cgcacccagc agcgagtatt tgttgttact
    44521 tgaccagccc gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa
    44581 aatcccgatg ttcaggtcgg caaccaccca acccggactg actggcacaa ttgcaaaggc
    44641 cagcagcagc gaggtaaagg caatcatcgg tgccagggta aagatgacgc gatccgagaa
    44701 tttcgggatc cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga
    44761 accgccccag ccaacacggt taggtccata acggttctgg aacagaccca gcaggcgacg
    44821 ttcgccaaag ctcatgaatg ccccacaggt gacaaccacc agcaggatcc ccaccgcttt
    44881 gaggatggtc agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc
    44941 ttgagatcct caagatgcgc gccagccagc accggagcaa tgccggacat acccatcggc
    45001 aagcccacct gccctgccgt cagtccttcg gcgatttcaa ccggcagcgt gaccgtattg
    45061 ccatcgtaac taaaggagac gcgtgtacct gcgttcacac ccaacttcgc ggcatccgct
    45121 gggttgagtt tgatgtacgg ctgcggcata cggctctgga agaccggagc acgctgtgac
    45181 aattcatcgc tgccaaacag gtggtagtac ggcgcgatac gccatttccc gtcctgcggc
    45241 tggaagcgtg ccggtacgct ggtgaagtaa tccagaccgt tttcgctggt ttcaaacaga
    45301 cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa tttgttccac
    45361 gcctgcgggg agttccagcc cggcgcccag gcaaacggca cttgcgaacg gtgcgcggtc
    45421 ggctggttgt taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc
    45481 ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca
    45541 cgggccagtt tctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgataccc
    45601 gccagttccg ggattttcgc cacaacagcg tcaattacat ggtcgagctg cgtccagtcc
    45661 acttcacggc tcagcaaggt gctgtgcagc gagtgcagcc agcgccagct ttccagcatg
    45721 acagttttgc tgtcgtaata agccggatcg taaacctgga agaaacgttg ggcgcgacct
    45781 tcattattaa ttaccgtacc gtcgctttca gcaaagctgg cagcggaaag caccagatgg
    45841 gcgttttcca tgatcgctgt gcgttgatga tcaaccacca tcaccagcgg cgctttagcc
    45901 agcgcagcat tcacgcgggt agcagaagcg taacgatgca gatcgttttc caacaccacc
    45961 accgcgtcgg cgcgtccggt ttccagttcg gttaacgctt cttcaagcga accgccaccc
    46021 ataatgccca gccccatgct gttgacggaa cgggcaatca tggtgatacc aacgtcagcg
    46081 ccgcgacctt tcagggcttt cgcgacgtta gccgcagcct gaatcacctc tatgctaccg
    46141 gcgttcgtcc cggagataat caacggtttc ttcgcacctg ccagtgcctg cacgatgacg
    46201 tcgattttgt tttgcagctc aggttcgata ccgtcaaccg ctggtgcgga gttatccagc
    46261 gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac
    46321 gccgcgatat catccagacg agtgtcatca acgttggtaa caaacagcgg atgcttcgca
    46381 cgttgaccga tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgcagcc
    46441 atttcgcgcg ctttaccttt caccgcctga cgcactgcca gtgcgacgcg cgcgccggtc
    46501 tgggtaacgt cttcgcccag caccagtacc gcatcgtaag attcgatttc gcgcagagcc
    46561 ggagtataaa tgccgccttc acgcagcact ttcagcgcca gttgcagacg ttcctgctca
    46621 ccgtgagcga taccggtgta gaagttttct tcgcccacca gttcacgcag cgcaaagttg
    46681 ctttccacgc tggcacgcgg agaaccaata ccgatcactt tcttcgactg acgcagaata
    46741 tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc
    46801 tgtactggct gacgcggacg atccttcagg ttgacgtaac cgtaaccgaa acgaccacgg
    46861 tcgcagagga agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttcg
    46921 ccgtaacgtt caccggggct gatgttacag ccgatggaac attgctggca gatgctcggc
    46981 gcaaactgca tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg
    47041 ccggtcgggc aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc
    47101 gggcgaccga agtagacgtt gtcgtgcgcg ccataaacgc ccagatctgt accgtcagcg
    47161 taatctttgt agtaacgcac acagcggtag caggcgatgc agcggttcat ttcgtgagag
    47221 atgaatggcc ccaaatcctg attacggtgg gtacgtttgg tgaaacggta gcgacggaag
    47281 ctgtgtccgg tcatcacggt catatcctga agatggcagt taccgccttc ttcacatacc
    47341 ggacagtcgt gcgggtggtt ggtcattaac cactcgacca cactttcacg gaactgtttc
    47401 gcttcttcgt cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc
    47461 accaggcgac cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa
    47521 gcaccgacgc ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac
    47581 agacaagctt ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga
    47641 attgtagcca ttagcatgct tccagttttc tcggtcaggg accgagcgtt aatcgaaatt
    47701 cggttaccag cgctctttca gcaggttcgg ctgaatccca ttaatcaaat gggtattgct
    47761 gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa
    47821 cggctccact gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca
    47881 cagttgctca agtgtttcga tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag
    47941 aattttcacg ctccacggca gaccgtcgcg gcacggcgta caccagccgc aggactcacg
    48001 ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc
    48061 catcgccagc gccgtaccca gacggctgcc cgctttaccg atactttcga attccatcgg
    48121 cagatcaagg tgcgcttcgg tcaggaagtc agtccccgcg ccgcctggct gccaggcttt
    48181 aaatttcaga ccatcacgca taccaccggc gtaatcttcg aggatctcgc gtgcggtggt
    48241 gccgaacggc agttcccaca gtcccggatt tttcacccga ccggagaagc ccatcagctt
    48301 ggtgccagca tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat
    48361 cgccggaacg ttacacaggg tttcgacgtt gttgacacag gtcggtttgc cccatacgcc
    48421 ggaggttgcc gggaaggggg gtttcgagcg tgggttagca cgacgccctt ccagggagtt
    48481 gattaacgct gtttcttccc cgcagatgta gcgccctgcc ccggtatgga cgaacagttc
    48541 gaagtcgaaa cctgttccca taatgttttt gccaagcaag cccgcttcag tggcttcggc
    48601 aatagcgcgg cgcagattta ctgccgcttc gatatattcg ccacgcagga agatgtagcc
    48661 acggtaagct ttcagcgcaa acgcggagat gagcatacct tccaccagca ggtgcggcag
    48721 ttgctccatc aacaggcggt ctttataggt gcccggctcc atttcatcgg cgttacacag
    48781 caggtaacgg atgttcatgg attcgtcttt cggcatcagg ctccacttca ggccagtcga
    48841 aaagcccgcg ccgccgcgcc ctttcagacc agcgtctttt acctgattaa cgatttcgtc
    48901 cggagacagc ccggtcagcg ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc
    48961 gtccagccac actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg
    49021 agtacggata atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc
    49081 agatgcgcgt gagtgtcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg
    49141 cagcaagttg gcagcagcgt aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc
    49201 agctttttct cgagcgccgc ctgaataccc tgatacccgt tgatatgaca gaccacgctg
    49261 tcgcaataac ggatcacatg gcgaccaacc ggctggcgga agatctgact gtagaacgtt
    49321 gccacacctt cgacgtcgct tgccggaata cccagcacat cggcgatcgc gtggatcgca
    49381 ccatccggca cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca
    49441 cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc
    49501 tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca
    49561 taacaaaatc gatactgccc agataaacaa tcaggtcaga caccaggctg ccgcggatcg
    49621 ccgccggaat ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg cgataactca
    49681 tggtgctacc gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaatcatct
    49741 ggaaagattc attggcaggc atcaccggcc cccacgacac ttgcaggaag tgggtgatca
    49801 gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg
    49861 ctttgaacgg gccttccggc atgttgttga ggcactgctc aagaatgcgc agactctggc
    49921 gcagctcttc cactttcagc attacgcggg tgtagcagtc agaaacacca ccaccaaccg
    49981 ggatttcaaa gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt
    50041 cgatcccggt agcacgcagg cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc
    50101 cataggcggc aacgccctgg gtacgacctt tcagaatggt attttgcagc gccgctttct
    50161 cgtaagacgc cagacgtttc ggcatccagt cgaggaactc acgcagcaga cgatcccagc
    50221 cgcgcggcag gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacggaaac
    50281 ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg
    50341 gcgtcattgc gccgacgtcc tgaataaagg tcgagatgta cagcaggtgg ctgttgatac
    50401 ggaacagttc ggagagcata acgcgaatga cgttaacgcg atccggcacg gtgatcccgg
    50461 ccagtttctc taccgccagc acgtaaggca tttcgttaac gcagccgccg aggtattcaa
    50521 tacggtcggt atacggaatg tagctgtgcc aggactggcg ctcgcccatt ttctccgcac
    50581 cacggtggtg gtagccgatg tctggtacgc agtcgacaat ctcttcgccg tcgagttgta
    50641 aaacgatacg gaaagccccg tgcgccgacg ggtggttcgg accgaggttg aggaacatga
    50701 agtcctcgtt ttcggtgccg cgcttcatcc cccactcttc cggtttgaag gtcagggctt
    50761 ccatctccag atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgcg
    50821 ccgggtaatc tttacgcagc gggtgacctt tccaggtttg cggcatcatg atgcggcgca
    50881 ggttcgggtg accgtcgaaa gtaatgccaa acagatccca ggtttcacgc tcataccagt
    50941 tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca
    51001 ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat
    51061 ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa
    51121 acagcatgac gtaaggtttc ggcagtttct ttaagaaatc gccaacttcc agtaattgtt
    51181 cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg
    51241 gcccaaaacg gttgcgcagt tcgccaatta ccggatcatc aagatgatcg cgggtctgcc
    51301 aggcgggttc ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt
    51361 gactgtcggc gcagtaagct tcgcgaaata gatttaggaa taagcgaaga aaaatccctt
    51421 tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt
    51481 caccgcgctt gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa
    51541 ccacccagga gagcggacga cgttctttgc cgatagattc ctgcaacagc atcagtgcct
    51601 gcatgtacgc ttcaggacgc ggcgggcagc ccgggatata cacatcaacc gggatgaatt
    51661 tatcgacgcc ctgcacaacg gaataaatat cgtacatacc accagagttg gcacaggccc
    51721 ccattgagat aacccatttt ggttccagca tctggtcata cagacgctga ataaccggtg
    51781 ccattttggt aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac
    51841 gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct
    51901 caacgtaaca gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt
    51961 taaccatgtc attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt
    52021 cggttacgat ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc
    52081 gggtgagcgt ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt
    52141 tcgtttccgg gttcatacac tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa
    52201 tacgcaccag ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa
    52261 agcctaccca gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa
    52321 cgtcgaagat aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg
    52381 cggagccgac cgaatcgata ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc
    52441 gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa
    52501 gaaagatagc gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg
    52561 cttactcatc aaaagtagcg ccagattcac tgctcttcac ggcagatgga cgccacatcg
    52621 attcatgggg aggaataaaa aaaaccttac aatcactgta gaaattcttt tatacagcta
    52681 attgatgtgg tcttttactc ctttctataa ccttttgtca actttaacaa aagtttcttc
    52741 acattagttt acataatatc aacaccatta gcatttaatg ccctttcacc ccagatcctt
    52801 gacgactcca ggataattag atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa
    52861 tacgggtcta ttttgacagg aatttgtgtc gattcctccc cccaaaagag agtattttct
    52921 tgatctgtga cacgcttttg ttattccata acaaaaacgc agcaacaaat ttacgtattt
    52981 tttaacatca ttgtagcagg tgattttttt caggcgatta tttgtgcgtt cgggacgtga
    53041 atctctggtg attgaaaaat aaacagtttt gtacgttctg cactatgcga ggaaggattt
    53101 ttactaaaaa aaagccgctg gggtttaaaa cacccccagc ggctcgtttt ttacactatt
    53161 gtctcaggaa ttatctatcg tccgtcgatt actcgatatc cctttcaatc aacaatgaat
    53221 catccccttc cggagcagac attgggctgt attgccacgg attatggtag ctctccatcg
    53281 cctgatagat cacctgggcc agctcattat tactggacgg gtcatagcac agcaaatatt
    53341 cggtatcagg tagcggcggt aagccatcta ctccactcag cacacgcagg tccgggctca
    53401 tcatctcaac cggccttgcc gtcacgccaa gaccggcttt cactgccgca cgaacggccg
    53461 gaagcgtcga ggcgacataa gccagtcgcc atggaatatc tgctttatta agcgtcgcca
    53521 gcaccatatc gcgaaacgga cttggatcat ccagcaatac aagagggatc ggctcgcctt
    53581 tttgcaatac gtattccgct gcgcagtacc agtgtgttgg cgaggtacgc aggttcaatg
    53641 ccttaaacgc tgaaggacga tgggtggtta ccatcaaatc cacttcctgg gattccagca
    53701 tttctgccat ataggcatta cgtttaacgc ggacatccag cgctaatttc ggataaaccg
    53761 aactcacgcg atttaacagg aaaggtaaga tcgtatcggc agattcatct gaagcaccga
    53821 tagttaacac gccctgaaga ttactgaaca ttaatgatga gcaggcctca tcattaaaac
    53881 gcaggatttt cctggcgtaa ccaagaagtt gaatgccatg ttcagttaac agtttgttgc
    53941 gaccgtgacg agcgaacagt tctttcccaa cgagttgttc cagacgctgc atttgctgac
    54001 ttacggcgga ctgagtacga cacacagcgg cagctgcggc agcaaaagtg ttcagatcgg
    54061 caacagcaac aaatgttctc agcagatcga ggtcgaggtt aattatcgga cgatttgcac
    54121 ttatcatata ttatcactta ctggcggctc atactgagct ggttaatgct gtgcacacac
    54181 aaacaagcaa ttccatttgt aatgtgcctc cctggcagtt tcatccggaa cccggacgaa
    54241 agtaaaaatg catatgagtt gcactaaaaa agcgactcac attgttccgt tataatgcct
    54301 gaagtagatc acagaatata tcttcaggga tcgcatatct attaagttac tcactctttt
    54361 ctatttatga catgcgcgtg tttgtataaa tgtaaatgtg agtccttgtt ccactctcgt
    54421 gcagcatcgc tggtcatacg cgaacacgta caaacagcaa tggtgaagcg gcatcaagag
    54481 cagggatccg tcatttatgc gagcatttta ccccaaaacc ttttatttat aagggtcatt
    54541 gcgaattatc tgatgcaaag ttatgttatg ttatgttatg ttatgttatg ttatgttagg
    54601 caaagtaatc ttctattatt aataagcaca tcaaaacctt tttgaatatt aaataataat
    54661 taattagcat catcctcatt cattaattcc gcttaacaat agtttcacac cttatcccca
    54721 ttgcgacacc tcggaaatca tcacgctgtt aaactctccg taatgcggaa atatcatcat
    54781 cgcgtttcat tagtgaattc ttaaatgagg cattttcaca caattatctt acagataaaa
    54841 aaaccagact tgcaattaag aattagaaca attcactata taacattaca tgtaaagcat
    54901 atacacctca ttattttgtc attattaagt tattaacagc acaatcgagc cttcccctct
    54961 ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca ttgttctaag
    55021 ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc atgtccccca
    55081 ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg gtgctgaaag
    55141 aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc ggcaacccag
    55201 ccccattcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc aacctgccta
    55261 ccgctcaagg gtattgcgat tccaaagggc tttactccgc gcgtaaagcc atcatgcagc
    55321 actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc ggcaatggtg
    55381 tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac gaaatgttgg
    55441 ttcctgcacc agattaccca ctctggaccg cggcggtttc gctttccagc ggtaaagcgg
    55501 tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat attcgcgcta
    55561 aaattacgcc tcgtacgcgt gggattgtta ttatcaaccc aaataaccca accggcgcgg
    55621 tatattccaa agagctttta atggagattg tggagattgc acgtcagcat aatctcatta
    55681 tcttcgccga tgaaatttat gacaaaattc tctacgacga tgctgagcat cactcaattg
    55741 cgccgctggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa acgtaccgcg
    55801 ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac gccaaaggct
    55861 acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt cctgcgcaac
    55921 acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt acccctggcg
    55981 gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt ccgggcgttt
    56041 cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc aaacgcttta
    56101 acattcatga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa gttctgttgg
    56161 tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc acgctaccgc
    56221 gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct ggttatcatc
    56281 agctgtaatc ttaatttcac tgccggagat tgcatccggc agtgttatcc cgccacaatg
    56341 acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt ttgcccatct
    56401 ctcccgcctg aaactcatta accgctggcc gctaatgcgc aacgtgcgga cggaaaatgt
    56461 gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta tcaaaaatcg
    56521 gaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt accacgatgc
    56581 ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatg cgcaaatcgc
    56641 tcaggagtac aaggctattg aaaaaatcgc tcagcagaaa ctggtcgata tggttccgga
    56701 agaactgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg atgaagaaaa
    56761 atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg aagaactcgc
    56821 ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc ttgaagcgcg
    56881 tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc atctttcgct
    56941 cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg catcaggcaa
    57001 taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg gatcatcacc
    57061 acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa gtcactgaag
    57121 ctgtaatttc ccggacctaa aaccagggtg ttaacaggtg aagacaccgg tgtcataaag
    57181 gcggcggatg ctgccatcgc cacgaccatc gcaaatggat aaggcgacac tcccatcgtt
    57241 ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt agagataaac
    57301 agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg cccataaccg
    57361 ccgccaatat ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt ctgtaatgcc
    57421 acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat ggatttatag
    57481 gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat gatagcggca
    57541 acaggattag gaatttcatt tgtcagcatt aacgccacca ttagcaccag acagaaaatg
    57601 gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg taagttcagc
    57661 gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt acccacaacc
    57721 aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag cgccacgcca
    57781 ttgcgcttga gccccaccac attcagtccg tagcgggtac gaaaaccaat ttcgcgcacc
    57841 gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc cacatcaagg
    57901 gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg ctcactacaa
    57961 aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc acgcgcgcga
    58021 aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca gcgttcaaca
    58081 ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg ttgacctatc
    58141 attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg atattcacgg
    58201 ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg ggtatcccct
    58261 ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac gaccaggcca
    58321 ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa ttcactgttg
    58381 actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag cccggcaaaa
    58441 cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac gcttaacacc
    58501 acgggaataa agatagcgac aacgctggtt gaactcataa acgcgccaag ccccgcgacg
    58561 gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt gaccagccat
    58621 gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat aaacaaggcg
    58681 gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac cgtcagcgtt
    58741 ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg cactctgccc
    58801 gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa ttcaccgttc
    58861 acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa agccccgacg
    58921 agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca tttggctgtt
    58981 tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc aggcgcgggg
    59041 tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgctaggcca gaaagcacgc
    59101 cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc agctgcgcac
    59161 ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg gttacaaaca
    59221 cctctggtgc gggaagcccc gctattttat ggcgcgctcg cgctaccggc atggagccag
    59281 aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag gcgattgccc
    59341 cagaaagcgc ggtaatacct tcggtttccg tggcctcgat ttgctccaga cgcgtaaact
    59401 cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcaaagag gtgatcgcct
    59461 gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt ctggcccagt
    59521 tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca agatcaaaca
    59581 gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt aatttcgttg
    59641 gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg ctgatatttt
    59701 tcccacattg gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg ggtatagcgc
    59761 ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt ggcggcgtca
    59821 aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa attagaccag
    59881 gaaacatgca acgcatgata catctccata atgtcgatga tagtgcggca ggtttcttct
    59941 ttcagctcgc caaactcgcg atccaattca cgcatctgta atccgtaacc acgctcaata
    60001 attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt catcatcttg
    60061 tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga ctcctgtatc
    60121 actctactac ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg cgtaatcagg
    60181 ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc gcttaagcgt
    60241 gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct gtccagactc
    60301 ctgcaattta ccgcgaactt cataaaacca gttaaggatt gcagggggta atggcgttac
    60361 agaacgcttg cccagccacc acaatccctg catgggtaaa cttaaggcga acagtgcagt
    60421 ggcaactgcc ggcccaagca gaccgcccag ggcaatctgc cagcagagag taaatacggc
    60481 gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat tttcgacaaa
    60541 gaccggggca aggcgttttt ccagtggcca cgtctttgag taatgctgtc cgcggcgaaa
    60601 caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc tcaacttcac
    60661 atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt tcaaaacatt
    60721 ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa atagtacata
    60781 ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac gcaaaatggc
    60841 atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg atgttaatca
    60901 taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt ttagccacgt
    60961 atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact gcggtagttc
    61021 ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt ctggtttagc
    61081 cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca ataaacagga
    61141 agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg ttaatactat
    61201 tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc gtatcgtaca
    61261 cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc agggtatcaa
    61321 agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta tcgaagaagc
    61381 tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca ccgcgttcca
    61441 ccagactatg ccggaagagt cttacctcta cgccctgccg tacaacctgt acaaagagca
    61501 cggcatccgt cgttacggcg cgcacggcac cagccacttc tatgtaaccc aggaagcggc
    61561 aaaaatgctg aacaaaccgg tagaagaact gaacatcatc acctgccacc tgggcaacgg
    61621 tggttccgtt tctgctatcc gcaacggtaa atgcgttgac acctctatgg gtctgacccc
    61681 gctggaaggt ctggtcatgg gtacccgttc tggtgatatc gatccggcga tcatcttcca
    61741 cctgcacgac accctgggca tgagcgttga cgcaatcaac aaactgctga ccaaagagtc
    61801 tggcctgctg ggtctgaccg aagtgaccag cgactgccgc tatgttgaag acaactacgc
    61861 gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg cgaaatacat
    61921 cggtgcctac actgcgctga tggatggtcg tctggacgct gttgtattca ctggtggtat
    61981 cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg tgctgggctt
    62041 tgaagttgat catgaacgca acctggctgc acgtttcggc aaatctggtt tcatcaacaa
    62101 agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa gaactggtta tcgcgcaaga
    62161 cgcgagccgc ctgactgcct gatttcacac cgccagctca gctggcggtg ctgttttgta
    62221 acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt cccgtattat tatgctgatc
    62281 cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg tgcaatggaa
    62341 cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac cggtggcgat
    62401 gcgcccgatc agactacgac tatcgtgcgt gcgaactctt ccaccacgac ggccgctgaa
    62461 ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca atcagaaaga tgtgctgatg
    62521 gaagagatcg tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt tctggttgaa
    62581 ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc tgaactacga aatcgctaaa
    62641 acgctgaatg cggaaatcgt cttcgttatg tctcagggca ctgacacccc ggaacagctg
    62701 aaagagcgta tcgaactgac ccgcaacagc ttcggtggtg ccaaaaacac caacatcacc
    62761 ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac agggtcgtac tcgcccggat
    62821 ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa acaatgttga tccggcgaag
    62881 ctgcaagaat ccagcccgct gccggttctc ggcgctgtgc cgtggagctt tgacctgatc
    62941 gcgactcgtg cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa cgaaggcgac
    63001 atcaatactc gccgcgttaa atccgtcact ttctgcgcac gcagcattcc gcacatgctg
    63061 gagcacttcc gtgccggttc tctgctggtg acttccgcag accgtcctga cgtgctggtg
    63121 gccgcttgcc tggcagccat gaacggcgta gaaatcggtg ccctgctgct gactggcggc
    63181 tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac cggcctgccg
    63241 gtatttatgg tgaacaccaa cacctggcag acctctctga gcctgcagag cttcaacctg
    63301 gaagttccgg ttgacgatca cgaacgtatc gagaaagttc aggaatacgt tgctaactac
    63361 atcaacgctg actggatcga atctctgact gccacttctg agcgcagccg tcgtctgtct
    63421 ccgccagcgt tccgttatca gctgactgaa ctggcgcgca aagcgggcaa acgtatcgtt
    63481 ctgccggaag gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc tgaacgtggt
    63541 atcgcaactt gcgtactgct gggtaatccg gcagagatca accgtgttgc agcgtctcag
    63601 ggtgtagaac taggtgcagg cattgaaatc gttgatccag aagtggttcg cgaaagctat
    63661 gttggtcgtc tggtcgagct gcgtaagaac aaaggcatga ccgaaaccgt tgcccgcgaa
    63721 cagctggaag acaacgtggt gctcggtacg ctgatgctgg aacaggatga agttgatggt
    63781 ctggtttccg gtgctgttca cactaccgca aacaccatcc gtccgccgct gcagctgatc
    63841 aaaactgcac cgggcagctc cctggtatct tccgtgttct tcatgctgct gccggaacag
    63901 gtttacgttt acggtgactg tgcgatcaac ccggatccga ccgctgaaca gctggcagaa
    63961 atcgcgattc agtccgctga ttccgctgcg gccttcggta tcgaaccgcg cgttgctatg
    64021 ctctcctact ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt tcgcgaagca
    64081 actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct gcagtacgac
    64141 gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga actctccggt tgcaggtcgc
    64201 gctaccgtgt tcatcttccc ggatctgaac accggtaaca ccacctacaa agcggtacag
    64261 cgttctgccg acctgatctc catcgggccg atgctgcagg gtatgcgcaa accggttaac
    64321 gacctgtccc gtggcgcact ggttgacgat atcgtctaca ccatcgcgct gactgcgatt
    64381 cagtctgcac agcagcagta atctcgtcat catccacagc tttgcgctgc ggatatctga
    64441 accggaaata atcactattt ccggtttttt attctcttaa tttgcattaa tcctttctga
    64501 ttatcttgct taactgcgct gcatcaatga attgcgccat ctcactttgc atacttacca
    64561 ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc caaaccaaca
    64621 agaagatggg caatgcccga tacgttggtg attatctttt ttgttgctat tttaaccagt
    64681 cttgccacct gggtagttcc ggtggggatg tttgacagtc aggaagtgca gtatcaggtt
    64741 gatggtcaaa caaaaacacg caaagtcgta gatccacact catttcgcat tctgactaac
    64801 gaagcaggcg aacctgagta tcaccgcgta cagctgttca cgacgggcga tgaacgcccg
    64861 ggcctgatga acttcccgtt tgaagggtta acctcaggat cgaaatacgg gacagccgtt
    64921 ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat gcgtacagga
    64981 accattgata acggtatcct ggcgcttatt cgccataccc gcgggaatga aattctcttt
    65041 attcctgcgc tgtttattct gttttcactt ggcggcgcgg tatttggtat gggagaagag
    65101 gccgtcgcct ttgccattat catcgcaccg ctaatggtcc ggctgggcta tgacagtatt
    65161 accaccgtcc tggtgactta tattgccacg caaatcggtt ttgccagttc gtggatgaac
    65221 ccgttttgtg tggttgttgc tcaggggatt gccggcgttc cggtgctttc tggctccggg
    65281 ttgcgcatcg tggtgtgggt tatcgccact ctgattggcc tgatctttac catggtgtac
    65341 gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga ccgcttcttt
    65401 cgtgaaaagc aggcggatgt tgaacaacgt ccgtttacct ttggtgactg gctggtattg
    65461 attgtcctga cagccgtaat ggtctgggtg atttggggcg tgatcgttaa tgcctggttt
    65521 attccagaaa ttgccagcca gttcttcacc atgggtctgg tgattggcat catcggcgtt
    65581 gttttccgcc ttaacggcat gacggttaat accatggctt catcctttac cgaaggggcg
    65641 cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca aagggatttt gctgctggtt
    65701 ggtaatggtg aagcaggtaa tgccagcgtg ttaaatacta tcctcaacag cattgccaat
    65761 gccattagcg gtctggacaa cgcggtcgcg gcctggttta tgttgctctt ccaggctgta
    65821 tttaatttct tcgtgacgtc cggttctggt caggcggcat taactatgcc gttactggca
    65881 ccgcttggcg atctggtcgg tgttaaccgt caggttaccg tgctggcgtt ccagtttggt
    65941 gatggcttca gccacatcat ttacccaacc tcagcttcgt taatggcaac gctcggtgtg
    66001 tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga ccctgcttgg actgctgttt
    66061 attatgtcca gcgtcgtagt gatcggcgct cagttgatgg gctaccacta aaaatgttaa
    66121 gagccgcaat tgcggctctt ttttcattct gccgtttcag tctctacggc ttcatttttg
    66181 gcattgcgct tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa ctcatcgcag
    66241 cgtgcggtga tttcttccgg cgtcagccag caaacttcac tgacttcatc ttcctgtagg
    66301 gcgaagggac cgtgagagac gcaactgaac aacgcgcccc agacacggca atttttatct
    66361 tcgaaataga actgcccgtg ctcggcaaag gggacaccgg caatgcccaa ctcttcttcc
    66421 gcttcgcgac gcgcggattc cagcagttgc tcatcggcct gaactacacc gcctgcggtc
    66481 gcatctaaca tgccgggtaa aaagtctttt gtctcggtac gacgctggac cagaattttg
    66541 cccatgccat catgcacgac gatgtaagtt gcacgatgac gcagacactg tgcccgcatt
    66601 tgttcccggc tggcttgtgc aatgacttcg ttctcttcat tgacaatatc cacccattca
    66661 gtacttgcca aacgacgctg ttccattatc aggaaacctt ctttttctgg cgctcttacg
    66721 gcgcattttg agttgtgggt aaattacgga ttaatcgcga cctgcgcaat gatactttga
    66781 tcattgagtg cgattacact aagtacgtca ttatccagca tgccataact cgccggatta
    66841 ccgcctttcg gaatactcac cgagccgggg ttgaagtgga aaatctcccc ccgctgttct
    66901 gccactggca gatgggtatg accgtacacc agcacatcgt tctggtttaa agcaggtaga
    66961 ttttccgggc caaaaagatg accatgcgtc aaaaacagac gttgtttttc cagtaatacc
    67021 tgttgccacg gcgcggttat cgggaaatgc agcatcattt gatccacttc gctgtcgcag
    67081 ttgccacgca cagcgataac cttatgtgcc acttcattaa gccgttcagc gactttggct
    67141 ggcgcgtaac cctccggtaa agcattacgc gggccgtgat tcaacacgtc gccaaggatc
    67201 accagccact gggcaccgct ttggacaaac aactccacaa cacgttccgt cgccggtaac
    67261 gacccatgaa tgtccgatgc aaacatcagt ttcatcactc actcctcgtc gaaaaaacgt
    67321 aagctatgat actggattct gtcgttgcta tcagccagat tgcttcgccg aaagtgcaat
    67381 aaaacgctgg acagacgctc gctgccactg gaatgtcgca taatccacca ggcgttctgg
    67441 cacctcatcg ccatgtagta ccaggcggtt aatcattagc gccagatcag tatcagcaat
    67501 gcaccattca ccaaataaat tcggctgacc cagtgctaac aaatgttctg ccatcgcgaa
    67561 cagtttctct gcactggctt ttccctcggc cgttagtggc gcttttttcg cccccgcaaa
    67621 gacaacatcc gtcggacgct cttcgcggat gggcatcaga tcgctgcgca gccaggcctg
    67681 aatctgtcgt gcacgcgcac gattttctaa atcaagcgga taaatacgtt cccaggtcgg
    67741 tggcgcaaat cgatcttcca gatactccgc aatggcagaa gattcactca actcaaaatc
    67801 atcgatttgt aataacggca cacggcgtgt ctgaccgtaa ccttgccacg tcggctgcaa
    67861 atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt tttcctgcaa
    67921 cgccacccag gcggataaca cataagggga gaaaaagtgg gcatctgacc aaagcgtgat
    67981 agcgggttta ctcattatgt cctcggcaat aaagggggct ggctatcaaa ctatagagcc
    68041 tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct atacttgtgg
    68101 tgttcagatg attaaacaac cggagctgca atgatcgatc tctatttcgc cccgacaccc
    68161 aatggtcaca aaattacgct gtttctcgaa gaagcagagc ttgattatcg cttgattaag
    68221 gtagacctgg ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc gcccaacaac
    68281 aaaattccgg caattgttga tcattctcct gccgatggcg gcgaaccgtt aagcctcttt
    68341 gagtctggtg ccattttgtt gtatctggct gagaaaacag gactcttttt gagtcatgaa
    68401 acgcgtgagc gcgccgccac attacagtgg ttattctggc aggtaggcgg actggggccg
    68461 atgcttgggc aaaatcatca ttttaatcac gcagcccccc aaaccattcc ttacgctatt
    68521 gaacgttatc aggttgaaac tcagcgtctt taccatgtac taaacaagcg gctggaaaac
    68581 tcgccctggc tgggaggcga gaactacagc attgcggata ttgcctgctg gccgtgggtt
    68641 aatgcctgga ctcgcctgcg aattgacctc gcaatgtatc cggcagtcaa gaactggcat
    68701 gagcggatcc gttcgcgccc tgccaccggg caggcactgc taaaagcaca actcggtgat
    68761 gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt atttcatcct aagtaaaaca
    68821 acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac cttcgtttgc
    68881 gtacgtttat cggaattaag gaagaagaaa ttaacaaccg tcaggatatt gttatcaatg
    68941 tgacgatcca ctaccccgcc gataaagcgc gcactagcga agatatcaac gatgcgctga
    69001 attatcgcac cgtaacgaaa aacattattc agcatgtaga gaataaccgt ttctctttgc
    69061 tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg gtgacgtatg
    69121 ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta tccatgacct
    69181 taagctggca gcgttaatcg ccatatcggg aggctgcatg aacatagtga tcaccggagg
    69241 gacgggatta attggtcgcc atttgattcc gcgtttgctg gagctgggcc atcaaattac
    69301 ggtagtgacg cgtaacccgc agaaagccag ttccgttctc ggccctcggg tgacactatg
    69361 gcaagggctt gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca acctggccgg
    69421 agaaccgatt gctgataaac gctggactca caagcaaaaa gagcgtctct gccaaagcca
    69481 ctggaatatc acgcaaaaac tggtcgattt gattaatgcc agcgacacgc cgccgtcggt
    69541 actcatttcc ggctcggcaa cgggctatta tggcgactta ggtgaagtgg tggttaccga
    69601 agaggaaccg ccgcataacg aatttactca taaactctgc gcccgctggg aagagattgc
    69661 ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg cgaaccggcg tggtactggc
    69721 accggacggc ggtattctcg gtaaaatgct gccaccgttt cgtcttggcc tgggcgggcc
    69781 gattggttcc ggtcggcagt atctggcctg gattcatatt gatgatatgg tcaacggcat
    69841 tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc cctacccggt
    69901 acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg catcgcccgg ctattttgcg
    69961 cgtccccaca accgccattc ggctgttaat gggcgaatct tcagtactgg tattaggtgg
    70021 acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct ggtacgattt
    70081 agaagaggcg ctggcggatg tcgttcgctg atgtggttta cagcaaacat ccgccagtta
    70141 actccctgtg ttacaggatt agtggctttg cgcgataaga tcgtctggtg aaagtcgggt
    70201 caccatcata actaactctc tgtctaaacc tctatccagc atctcctgag caatacgcag
    70261 ggcttcttcg tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag caatggtcat
    70321 cagtttctcc ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg cacggaaacg
    70381 ctgggcatcc cctgtttgta atacgtaatt aaacagggct tttagctgtc tgtcattagt
    70441 gttccctgta actagcagcg aaacaatttg gtcgactaat cccaacagat cgcgctgacg
    70501 aatatgtttc tgaattaact ccaacagcgc cattttgcgg tgttgcataa tctcgtcatc
    70561 cggcaccacg gtaatatcca ccaacggaaa cgctgatgaa tatattttgc gggctatagc
    70621 aggctcggca aattcatcaa gccagcagag tgaataagga taaggacttc tgcaaccatg
    70681 ataaaacagc atcgggatca ccaatggaag ctctttatag cccgcatcaa gatggttttg
    70741 cattgccgca atggaataac gcatcatgcg aaaagccatt aattcttccg gcttactttg
    70801 gtgctctatc actacataaa tataacccac tccctcctgc gttttcacag accacaagag
    70861 gtcggaataa tattgccgca ggtcttcatc aataaaactg tttggttcta gtttaagcgt
    70921 cgttaaatca cacagtttgc gcagcggcgc gggaagatga atattaataa aattccgcgc
    70981 ggtgtctgga tggcgtaaaa aagatttaaa taccgcatca tgcggcgtgg aagttgtcga
    71041 tattgtcatg gcgttccgtc acccctcaaa tttaagatga cgcgacaata accacgatta
    71101 acacccaatg cagcaggcag tttttatctt tggacagcga attcagaagt atttttagcg
    71161 gactgcaact tttacatttc agcggaagct gactcgcaaa ttccccttga aaatactccg
    71221 gcagcgttag ccaccggaga ggaaattatt tcagcgatcc cttaaggaac tgttgcagac
    71281 gagggctttg cgggttgcca aataactgct ccggcgcgcc ctcttcttct attttcccct
    71341 gatggaggaa aatgacgtga gtagaaacat ggcgagcaaa gcccatttcg tgagtcacta
    71401 ccaccatggt tttcccctct tctgccagtt gctgcataat acgtaacact tcgcctacca
    71461 gttcaggatc gagcgccgag gtaggttcat caaacagtaa aacttctggt tccatcgcca
    71521 gcgcccgcgc gatagaaaca cgctgttgct gaccgccgga aagatgcacc ggatatttcc
    71581 cctgcgcacg ttcgtctatc ccgactttcg ccagatactt caccgcccgc tcgcacgctt
    71641 cctgcttgct caggcccaac acctgaatcg gcgcttccat gacgttttcc aacaccgtca
    71701 tatggctcca gagattgaag tgctggaata ccatcgtcag gcgtgtgcgc agtaagcgca
    71761 gttgattttt atcggcgact ttgagttgac cgtctttgtc gcgcaccaga ttgatcgtct
    71821 ggccattgac cacgatcgat ccttcactcg gtttttcgag gaagttaatg cagcgcagaa
    71881 aggtactttt ccccgatccc gacgatccga tgatgcttat tacatctccg gcattcgctt
    71941 gcagtgagac ccctttcagc acttcgtgtt cgccatagcg tttgtgcaaa tcgataacgt
    72001 ttaatttatt ctcggacatc gtattctcag tgcgttgaag aaggtttcac atgctgcaac
    72061 cagcgttttt ccgctctgcg aaagaggctg atcaggacat aagagatgat taaatagagc
    72121 accgcggcaa tgccgaaggc ggtaaaaggt tgatacgtgg cggcgttaat atcgcgggct
    72181 attttcagca gatccggcac agtggccgta aacgccagcg cagtagagtg cagcatcagg
    72241 atcacttcgt tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa aataatgcag
    72301 cgatacattt taaaggtcga gaagccatag gctctggcgg cttcaatttc cccatgcgga
    72361 accgaacgga ttgccccagc aaaaatctcg gtagtgtaag cgcaggtgtt aagcgtcagc
    72421 gccagcacgg tacagttcag gccactgcgg aagaaagcgt taaggaattc ggttccctta
    72481 acaatctcaa gcgtgtacat gccggaatag aacaccagca actgaacata cagcggcgta
    72541 ccgcgaaaaa tataggtaaa taaccagatt ggaaactgga tgtatttatt actggagaca
    72601 cgaccaatcg ccagaaacag cgccaggact ccgcctatca ctaccgacaa aataagcagc
    72661 cacagagtga tcgccacacc agtaaagcga taaccgtcgg tccacagcag cggtttccag
    72721 tattcatgta agatttcgat cacaggtcag ccctcttcac acccacggag tagcggcgct
    72781 caaggaacag cagcacacca ttggaaacgg tggtgaaaac caggtaaatc acgccacaga
    72841 cgatggcgaa atagaacggt tcccaggtac ttttgcctgc cagctgcgtg gctttgacca
    72901 catcttccag gccgagtaac gaaaccagtg cggtagattt gaggatcacc tgccagttgt
    72961 tgccaatgcc tggcagggcg taacgcatca tcgccggaaa catgatcctc cgaaacactt
    73021 gcccgcgagt aaaaccgaac gccgtcgccg cctctatatg ccctttcggc actgccataa
    73081 acgcgccacg gaacgtttcg gtaaaataag caccgtagat aaaaccgaga gtgataatac
    73141 cagcgaccat cggatcgata tcaatctgcc cgacgcccat cgcctccgtc accatgttta
    73201 gcgcaatctg caaaccatag aaaatcagca gcatcaacac caagtccggc acgcctcgaa
    73261 tcagcgtggt gtacccttcg aaaatcagcc ccaaaagccg attttgcgag agcttaccgc
    73321 cagcgccaat taaaccgatg attacagcga gcactacaga gctgatagcc agctccagcg
    73381 tgacgagcgc accctgtaaa ataacacctg aaaacccata caacatgctg cctgtcctgt
    73441 cgtgtgtggt gaattaccgc ctcttgtctc cctccggtta cccgaaggga gcgggctttc
    73501 tttcaccgat ggcgattaac caccataaac atcaaaatcg aagtactttt tcgctaattt
    73561 ctcgtaagta ccgtcagcgc gcatttcggc aaaggctttg ttcagtgcct cgcgcagttc
    73621 gttatcttct ttacgcaggc ccatgccggt ccctacgcca aacagttttt catctttaac
    73681 agacggacca ccgaatttgt aatctttacc gacgggttgt ttgaggaaac cttcgctggc
    73741 agcgacctca tcctggaacg cggcatcaat acgtccggca gtcaggtcag aataaatgtt
    73801 gtcctgcccc tgatacgaga cgatttcaat gccttttggt gcccagtgtt cattaccgaa
    73861 cgtctcctgg gtggtgccct gcaatacgcc tacccgtttg cctttcagcg actcgactgt
    73921 cggctgaatg tcagaatttt tcgccaccac cagacgagaa tcggcagcgt acagtttgtc
    73981 ggtgaaggca atttcttgct gacgtttttc cgtaatggaa agcgatgaca tgatggcgtc
    74041 aatcttcttc gcttttaagg acgggattaa cgcatccagc gggttttcga caaacgtaca
    74101 ttgcgtattg atgcgcttgc acaactcctt cgccagctcg atatcgaaac caaccagttc
    74161 gccttgtgaa ttttttgatt caaatggcgc ataggtcggg tcggtaccga tgcggatgtt
    74221 ttgcggaatt gcagcaagtg ccgcagttgc gctggagaag gccagaacca gagagagcga
    74281 tagcaccagt tttttcataa tgtcctcaac tgacagtctt tttataggga atatttacag
    74341 gtttgttaca cttatcgtgc cataaaattg accatcaagg caaaatatcc cgccccgtta
    74401 gacgtattat ttttcattaa aaatgcttaa gtatgcattt caatgcacca cgatagtgca
    74461 acaaacctgg cgtgcaccat gatgatcatt ccatcaggta cagcttccca gcgacgtatc
    74521 agtcaccgta gacattaaag tcgaaatact ttttcgccat cttgtcgtag gtgccgtcct
    74581 gacgcagctc gccaagcgcc ttattgaagg cagccgtcag ttcagcatca tctttacgta
    74641 gccctacacc ggtgccatca ccgaagtatt ttttgtcttt tactgatgag ccagcaaagg
    74701 cgaaatcttt accagcaggt tgcttgagga atccttcgct ggcagcaact tcatcttgta
    74761 acgcagcatc cagacgtcct gcagccagat cggaatagac caaatcctgg ttggcatagg
    74821 ccaccacatc cacgccttta ctacgccagg tctcgttagc gtaagcttcc tgggttgatc
    74881 cctgcagcac accaacatgt ttacctttca gtgaatccag cgttggctga atcggtgaac
    74941 ctttggcagc aatcaaacga gaatctgcgg cgtacagctt gtcggagaag gcaatctcct
    75001 gctgacgttt atcggtaatg gaaagcgacg aaataatagc gtcgattttt ttcgctttca
    75061 gtgaggggag cagcgcgtca aagtcactgg caacccaggt acatttcacc tgcatccgtt
    75121 tgcacatctc gttaccgaga tcgatatcaa agccaacaaa atcaccttta gcatctttcg
    75181 acgagaacgg cgcataggtg gtatcggttc cgatacgtac cgtctccggt agcgccgcat
    75241 agctggaagc cgctgcggag agaccgacta ataaagacag agcgagaatc gacttcttca
    75301 tacataaccc tcaagtgaaa atggcttttt tatgttgtgt attgtgttgt gtgtttgcag
    75361 gctctttcat gcaggtctta tgccatcttg acgacaacag taacattcaa cgttaaatat
    75421 gttaataaga cgttgcatta ttgtcctgaa gttgaagata gcaggtatag cggttgaatc
    75481 gcagcgtttc gattgaatgg cagaaacaaa atgtcgagga tttgatcgcg gttgcaaaat
    75541 tgccctgaaa cagggcaaca gcggagttat gcgccctgcc agcgggcaaa gagatcttca
    75601 ggaagggtta ttgcaaactg gtcaagaaca cgattaaccg tctgatttat cacatcatca
    75661 agggattgcg ggcgatgata aaacgccgga acgggaggca taatcaccgc accgatttct
    75721 gccgcctgag tcattaaacg cagatggcct aagtgcaatg gtgtttcacg cacgcagagc
    75781 accaacgggc gacgctcttt cagcaccaca tctgccgcac gggtcagtaa gccatcagta
    75841 tagctatgga caatgccgga cagggttttg attgaacagg gtaaaatcac catccccagc
    75901 gtctggaaag aaccggaaga gatgctggcg gaaatatcgc gcgcatcgag cgtgacatca
    75961 gctaatgcct gcacttcgcg cagggaaaaa tccgtttcga gggataaggt ctggcgcgcc
    76021 gcctggctca tcaccagatg cgtttcgata tctgtgacat cgcgcagaac ctgtaataag
    76081 cgcacgccat aaatcgcgcc gctggcaccg ctgatgccta caatgagtcg tttcataaat
    76141 agttgccctg tcagacttgc aggcagactt tgcagtattt cggcgggagt tgcaagtcag
    76201 ggtgccagac cggcaccctc agcgaaggca tcatccttcg ttatgcattt cgagattttc
    76261 cacttcgttc tgacgttgca ctgctttggc gtcatcatta cgtaacgtat cgaggaaatc
    76321 gaggtagccc tgatcaacat ctttggtgac gtagacgccg ttgaacaccg agcattcaaa
    76381 ctgctggata tccggatttt cagcgcgaac ggcgtcgatc agatcgttca gatcctggaa
    76441 aatcaacccg tcagcaccga tgatctggcg aatttcatca acttcgcgac cgtgagcgat
    76501 cagttccgtg gcgctcggca tatcaatacc ataaacgttc gggaagcgaa tttccggtgc
    76561 cgcagaagcg aggtacactt tcttcgctcc ggcttcgcgt gccatctcga taatctgctc
    76621 agaagtggtg ccacggacga tggagtcgtc gaccagcagg acgtttttat cgcggaactc
    76681 ggcgcggttg gcattcagtt tacggcgcac ggacttacga cgcagctgct ggcccggcat
    76741 gataaaggtg cggccaacat agcggttttt aacgaagccc tggcggtacg gtttgcccag
    76801 aatacgagca atttccagcg cgatatcaca cgaggtttct gggatcggga tcaccacgtc
    76861 gatatccaga tcttcccatt cgcgggcaat tttctcgccc agtttcgtgc ccatattcac
    76921 acgcgcgctg taaacggaaa ttttgtcgat aaacgagtcc gggcgggcaa agtatacata
    76981 ctcaaacagg cacggattgc tgaccggatt gtcagcacat tgacgggtaa acaactgccc
    77041 ttcttcagtg atgtaaatcg cttcgcccgg cgcgacgtca cgcaggaaat caaagcccag
    77101 cgtatcgagc gctacgcttt cggaagcgac catatattct gtacggttct cgtcaatatc
    77161 acgttttccc agtaccagcg gacgaatccc gtttggatcg cggaaagcaa ccataccgtg
    77221 gccgataatc atcgccacac aggcatacgc gccgcggatt aagcggtttg tggcagcaat
    77281 ggcagcgaaa atattgtcgg cttccagcgg gtagtggcgg aagttgtcca gctcgctggc
    77341 gaagatatta agcagaattt ccgagtcgga agtggtgttg atgtggcggc gtttttcttc
    77401 aaacagtttt ttacgcaact cgtgagcgtt ggtcagattg ccgttgtggg caagcgtaat
    77461 gccatacggg gagttaacgt aaaacggctg cgcttcagag gcgctggagc tgccagccgt
    77521 agggtaacgc acatgaccaa tgcccatatt gccctgcaaa cgctgcatat ggcgagcttc
    77581 aaatacatcg ctcaccagcc cgttcgcttt acgcaaacgg aagcagttat tggcatctat
    77641 ggtgatgatg ccggcggcat cctgaccgcg atgctgaagc accgttaagg catcataaat
    77701 cgactggtta accggcataa caccggcgat accgacaata ccgcacatac gtctttttcc
    77761 tcgttaagcc acatctcaga gcacttacgc tctgggcaag aaacttgacg agctttgcag
    77821 ataatcaaaa aaccatctga tgataaaact gaattgcggg atcagctgtg atttgctcca
    77881 gtcttcactt ttcgacaccc cggtaaagga gtcgagaaag aagagaatgg cggcaacaat
    77941 caacacaccg cgcaacgcac cgaaacagac gcccagcacc cgatcggtgc ctgacaaccc
    78001 cgttttctcc accaactggc ctatcacgaa gttcacgata gcaccaacga tcagggtagc
    78061 gataaacagt accgcgatgg caatcccatt tcgaaccagt tcgtcttcaa agcccgtaaa
    78121 ccagactgac aggtaagtgt agtaatgact ggcaacaaag aaagcacaac cccatgtcac
    78181 cagcgataac gcttcacgaa caaagccgcg gatcaggcta accagagagg aaaaagcaat
    78241 caccgcgatt atggcgtaat caatccagac catatgtgtc ccacgatttt acgccctgtc
    78301 atcctgttcg gggcgcattc taacagaaaa agaaaacgtt tgcgtaggga tttccttccc
    78361 gcgcatcaat aaaaatggcg ctgaaaaaat attcaacgcc atcgactttt tatgcctttg
    78421 cggcatcgtg caatgcgtgt cggatgcggc gtaaacgcct tatccgacct acggttctaa
    78481 cactgcccaa gcctgataag acgcgccagc gtcgcatcag gcaagactgt gttaattcgg
    78541 cgtatagccc attaccacgc cacttaagcc agaaagttgc ttcaactcac ccagcgaacc
    78601 tttcagctta tctttcgagg catccggccc aaccagaata cgggtaattt taccctgcac
    78661 tggcgtggat ggcgacgtat aaacccgata accggcaccg cgcagcttac cgacaatctc
    78721 attcacttta tcggcatttt tcagcgcacc cagttgcaca acataggctt tacccgttgg
    78781 cgctgctttt tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac
    78841 ctttggttta ggcggctcca ccggtttcgg cttcggtggg gcgaccggtg caggttccgg
    78901 ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg agcgacggtg ctgcggcatc
    78961 acctgcccgc acctcttccg ctgcgccttc cggcggctgc gtcggcaacg cctgggtggc
    79021 ggctggcatc atatcaggct catcacgatc gcccgctttc ggcaccagcg ggatagccgc
    79081 gaactcatcc tgataatgtt ttttctgccc gtccagcagc cctggaagta caatcacccc
    79141 cagcgccacc agcacgatcg tgcccactaa ccgattctga aacttacttg ccaccgcttc
    79201 tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg gaaagaacca cacaccagca
    79261 cggtgtcttc cgctttagcg tccgccattg cggcatccca tgcctgcgca acgctatcaa
    79321 atgatttgcc gttacccaaa tgctcaagca gttgttctgc cgtggcaccg cgcggccctt
    79381 ccagtggcgc acaataccag tcatcaacca cgcttttcaa ccaggccaga gttccggcaa
    79441 tatctttatc atgtagcata ccgataaccg ccagcacgcg cccgtttttc ggtagcgctt
    79501 tcatacgccc ggtgagatat tccgccgcat gtggattatg cgcgacatca aaaataacgc
    79561 gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc
    79621 gaatggcatt ttcactgact tccagcccgc tggcacgcag tgccgccagc gccgttgcgg
    79681 cattcggttg cgggacaagc ggcaacggca gattttccag cgtgccgtga gcatcgctaa
    79741 acgcccagtc atgatcggtg acggaatagt tccactcaac gccccgacgt tgtaacagtg
    79801 cacctttttc ctgcgccaca tcagcaatgg tagaaggcat ttccggctca ccgacaattg
    79861 ccggtttttc gctgcggaag atgcctgctt tctcgcgacc aatactttcg cgatctggac
    79921 ccagccagtc ggtatgatcc agcgcaatac tggttactac cgcggcatcg gcatcgacaa
    79981 tattggttgc gtccagacga ccgcccagcc ctacttccag aatcaccacg tcaagttgtg
    80041 cctgcttgaa cagccacaac gccgacagcg taccgtactc gaaataggtc agggaaatat
    80101 caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc ggcaattccc
    80161 ggccctgcac acgtacgcgc tcggtataac gcaccagatg aggcgaactg tagacgccca
    80221 ctttgtaccc tgccgccatc agaatcgact ccagcgtacg gcaggtggtg cctttgccat
    80281 tcgtacccgc aacggtaaac acaaatggcg ctggtttcag gacgccaaga cgcgccgcga
    80341 cctggctcac gcgctcaagg ccgagctcga tagttttact gtgcaggttt tccagataag
    80401 aaagccacga agccagaggc gacgcggctt gaggagtgcg tttgataatc atggtatccg
    80461 ctgattcgtt acggtgagaa tagcaaaagg gcagagccag tggccctgcc cttatcagtt
    80521 atcaggcctc aggttcctga tccggtaccg ggggtaccac tacgccttca cgcggcgctt
    80581 caggattcgg cgctggcaga ttcatcaact tcgccagaat gctcgccagt ttcaggcgca
    80641 tttccggacg acggacgatc atgtcgatcg cgcctttctc gatcaggaat tcactgcgct
    80701 ggaatctagg cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc
    80761 cgattaacgc tttaggttca gcgatgttga gatcgcccag catggcgaaa cttgcagaaa
    80821 caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc tcctgcattt
    80881 ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga catcagtgct tcctgcatac
    80941 gtgcgccacc agaggcggag aagcagatca gcgggcagtt atcttcctgc gcctgctcaa
    81001 cggcacgcac gaaacgtgca cccacaacag accccattga accgcccata aaggcgaact
    81061 cgaatgccgc agcgacaacc ggcattccat acagagtgcc tttcatcacc accagcgcat
    81121 ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc tttatacttc ttggagtcac
    81181 gaaacttcag ctcatctttc ggctcaagct cgctacccag ctccacaagg cttccttcat
    81241 ctaacaggct atgcaggcga ttacgcgctg tcatacgcat gtgatggtca cacttcggac
    81301 agacctcaag attacgttcc agctcagcgc ggtataaaac ctgaccgcag ctatcacact
    81361 tagtccacac cccttcagga atgctcgcct tgcgggtggg agtaatgttg cttttaattc
    81421 gttcaatcca gctcattagg gacctttctg tctgaacctg gttcgatgcc agttttatct
    81481 ttggggacgc ataatgccat ttttgccccc aacagaccat gaatgttgca cattaaaaca
    81541 taacagcccg aaactttgga taaaaaagtg gtcgaaccgc ggagttactt tttattttgc
    81601 ggcgcgtgcc gcagcgcgtt tgtgacggat tatttcgatg acgcccggca aaatagaaac
    81661 cacaataatc ccgacgatca gcagcttaag gttatcctga accatcggga ttgtaccgaa
    81721 gaaatagcct gcgtaggtaa aaagcagtac ccacaacagt gcgccgatca cgttataagc
    81781 agcgaaatga cggtacgaca tgtggcccat tcccgcaaca aacggggcga acgttctgac
    81841 gatcggcaca aaacgggcga gaataatcgt tttgccgcca tgtttctcat aaaactgatg
    81901 ggttttgtcg agataactgc gacggaaaat tttcgaattg ggattactga ataacttttc
    81961 accgaacaac cgcccgatag tgtaattgac cgcgtcaccc acaatcgcgg cgatcagcat
    82021 cagcactacc atcatatgga cgttgagatc gttggtttcc agtgatgcca acgctccggc
    82081 aacaaacagc aatgaatcac ccggtaaaaa cggcattacc accagaccgg tttcacagaa
    82141 caaaattaag aacaaaatgg cataaaccca gacgccgtac tccgcgacta gctctgccag
    82201 atgcacgtca atgtgcagga taaaatcgat gaggaaataa atcaggtcca tatttgccta
    82261 tgccttgtac tcgtcatctt tcaggctgta actacgttgg ctacagcctg aaatgttccg
    82321 agcattattc tcgttagtcc gccagaaata gcgggcccat tggcggtttt ggaagatcat
    82381 accggtcagg gtaatccacc gcgaccagat acagcccttc cgcttttgcc gttgctgccg
    82441 ccagcgttct gtcctttgcc gccagcaact ctgctatcca gctctccggc tggttgtggg
    82501 caccgacttc catcaggctg ccgacaatat tcctgaccat atgatgtaca aaggcattcg
    82561 ctttgatatc aaccaccaca taaggaccgt gacgcgtgac attaatatgc ataacgttgc
    82621 gccacggggt tcgggactgg cactgcaccg cacggaacga ggtgaaatca ttctcgccca
    82681 gcaagcattg cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta
    82741 cccctttact cagtaccgcc gggcgcagcc gatgattgta gatgatgtag cgataacggc
    82801 gagccgtggc gctaaatcgg gcatgaaaat catcaggtac agctttaacc caacgcacgg
    82861 cgatgtcacc aggtaaattc gcatttacgc ccaaggtcca cgccgcgtct ttgcgctgcg
    82921 cggttgtttc gaaatgcacc acttgcccgg taccgtgtac ccctgcgtca gtacgcccgg
    82981 cgcagaagac ggtgatgggt tcgttcgcca cctgggagag cgccttttcc agcttctcct
    83041 gcacactgcg gacttcattc tgccgttgcc agccgtaata cttactgccg tcgtactcaa
    83101 tgcccagcgc aattttataa actggcagtt gttgctggtc ggacattagt acagatactc
    83161 ctgcaccagt ttctcggcga tttttactgc catcagcgcg ccgccaaagc gaacgttatc
    83221 ggccaccgac cagagctgga cttgctccgg cataccgtag tcattacgca cgcagccaac
    83281 agaaagatgc ggcgtacccg aagcatcccc tacctgagtc gggaattcgt tctcttcaga
    83341 gagcacaata tcttcgccct gagcaaacgc atcacgcgct tcttctgccg ccagcggacg
    83401 cagtgcttca aagttgacca tctgggcatg accgtagaat accggtgcct ggacgacgct
    83461 tgccgaaatc atcagccctt cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg
    83521 ttcttcacgc acgctacctt cgctatccgg caacaacggc agcatattga acgccagttg
    83581 acgaccgaag aaatcttctt cgtcaatcgg aatgccgttg agcaatttcg cactctgccc
    83641 cgctaacgca tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc tggtaacact
    83701 gatacgcgac aaaccgccct gatcgattaa cggtttcagt gccgccagca gctggctggt
    83761 cagactgtct ggtacggcga tgacattccg gttgcggtaa tcggtcagta caaacgggtt
    83821 tacttccggc accaccagcg gtacgtcggg ttcgagagca aacagtccgc tgctgtcgat
    83881 caccaggcaa cctgagttgg tcgcttcttc aacccaggca gcggtagctt ctttgcctgc
    83941 gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca gcggcatcct gcacggtgat
    84001 tgttttacca ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca gtgcataaat
    84061 ttccccaacc gggaactgac gttcagccag cgtttcaagc agggcttcgc ccatagcgcc
    84121 agttgcgccc aggacggcaa tgttccagcc ttcagacatg gtggtttact ccagaaatag
    84181 caaagctccc tgccaatgtt acagcagaga gcatgaagaa gagattaacg tgccggatga
    84241 tgaacggcgt taaaacccag tttacacagc aatgatgccg cactggcgtc atcacaaatt
    84301 acatacagag atgaccattc acggcgctca agatagtttt tgcgcagttt atcgaactca
    84361 cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa
    84421 tgcaccagcc ttttcagcgt cggttgatcg agcggaccat gcagcgtaat gcggccaaac
    84481 tcaggcgcgg gcagcaacgt atccagcgca acgtgttgtt catgcccaat aaacttgcta
    84541 taggcttcaa acacttgcgt cgtgccacgc gctttaccct ccaaggtata gcctgcgata
    84601 tgcggcgtgc cgatatccac ttttgtcagc aattccacat taagttccgg ctcgccttcc
    84661 cagacatcca gcaccacgct taacttctgg ccttcattca ggcaggtcag cagcgcggta
    84721 ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg ctccgggctt caggctacgg
    84781 atcagttttt catccgccag atgtagcgtt ttgtacggac catctttaaa gagtggcgta
    84841 tggaaagtca gaatatccgc gcgctggact aactcatcca gcgagcggaa atcaccctca
    84901 tccccacggt cggcgcgagg cggatcgcaa agtaaggttt taatccctaa cgcttccagt
    84961 cgcgcctgca atcgacgtcc aacgttacca acgcccacga tccccaccgt acggtcgtgc
    85021 agtgaaaatc catcgcgttc ggcaagcatc agcagggagg agaaaacata ttccaccacc
    85081 gcaatcgcat tacagccagg tgcagcggaa aaaccaattc ccgcctgctt caaccatgct
    85141 tcatcgacat ggtcggtccc cgcagtggca gttccaacaa atttaatggg ttttcctgcc
    85201 agcaaagatt cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt
    85261 tgagcgacgg ggattggacg cccgggaacc gcgatcacct cacccaaacg gctaaataag
    85321 tcgcgggcat aaggcatatt ttcatcaaca aggattttca cgtttgtgtt acctgtatga
    85381 gacgagagtt aaccggacaa gtgtgccata atctcgcggc caggcatact tgcgaagatt
    85441 tcaggtataa ggatacgtaa tgatacaacc tatttccggc cctcctcttg ggcagccacc
    85501 aggtcaggga gataacctac cgtctggcgc gggcaatcag cctttatcca gtcagcaacg
    85561 tacttcgctg gaaagcttaa tgacgaaagt gacctcactg acgcaacaac aaagagcaga
    85621 actgtgggcg ggtatcaggc acgatattgg tctgtcggga gattcaccgc tgctttcgcg
    85681 tcacttccct gccgctgagc ataatctggc gcaacgtctg ctggccgcgc aaaaaagcca
    85741 ttctgcccgc cagcttttag cgcaattagg ggagtattta cgtctgggga ataatcgtca
    85801 ggcggtcacg gattatatcc gtcataactt tggtcagacg ccgctgaatc agctctcacc
    85861 ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc
    85921 acagcagcgc gaggcgaccg accgtccttt attaccggcg gagcacaatg cgctaaaaca
    85981 gctggtgacc aaacttgcgg cggcaacggg ggaacccagc aaacagatct ggcaatcgat
    86041 gctggaactt tccggggtga aagatggcga gttaattcca gcgaaactgt ttaaccatct
    86101 ggtgacctgg ctacaggcgc gtcagacgct aagccagcaa aatacgccga cgctggaatc
    86161 actacagatg gcgctaaaac aacctttaga tgccagtgaa ctggcggcgt tatcggcata
    86221 tatccagcaa aaatatggcc tttctgcgca atcatcgctt tcttctgccc aggccgagga
    86281 tattcttaat cagctttatc aacggcgggt taaagggatt gatccgcgtg atatgcaacc
    86341 gctgcttaat ccttttccac cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc
    86401 gctgtggata ctgttagtcg cgattatcct gatgctggtc tggctggttc gttaaccccg
    86461 acgaaatgac agtatcgtga caataatacc cagcaccgca gagatcgccc cggcaagaaa
    86521 taccgaagag taaccaaacg tggtcgccag cattcccgcc agcggcccgg agacgccgag
    86581 ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac
    86641 gcgtttaacc acctccacgc ccagcgcagg aaagataagc gaacatccgg ctccggttaa
    86701 cgccgcgccc gctaatgcga cccatgcacc tggggcttgc cagagcagca acaagcccac
    86761 cgtttctaca agcagagaga caatcgccac tttcacgccg ccaaaacggt ccggcatcca
    86821 gccaaacatg acgcgcatca cgacaaatgc gccgccaaac gcggtaagag taaagcccgc
    86881 catcgcccat cctttgctgg caaagcagag cgaaacgaaa gtcccgataa ccgcaaaacc
    86941 aacgccttgt agtgccagcc ctaaccctgg tttccagata agcccgacaa cgctccacag
    87001 cgatggacgt tctcccgcca gggccggtac tttgcgcact gtgccgttac aggcccacgc
    87061 cagtaagggt aataccattg tggtgatcgc cagtgcggca aaaccgtaat ggctatgaat
    87121 caacaggcca agcggcgcac caacagcgag ggcaccgtaa atcgccattc cgttccatga
    87181 catcactttg ccagagtgtt ttggccctac gatgcctaac ccccaggtca gagcgcctgt
    87241 cagtaactgg ctttcaccaa acccaagaat caaacgcccg atgaccaaca gggcaaattt
    87301 gaacggtgcg gagacaggca aaatcgccgc cagcagcaac gcgccgccag ccagaccaca
    87361 agctaacatc ccctgaagcg ccgaacgttt tgcgccatat tgatcggcca gtcgcccggc
    87421 gtaaccacgc gtcagcaccg tagccagaaa ctgaatcccg acggcaatgc cgaccatggt
    87481 attgccatag cccagatcat gatgaacaaa cagcgggata accggcaacg gcaaccctac
    87541 ggtcatgtag gtgagaaaaa ccgcaaaagc gatgcggaag agcgaaaaac tggcagaaga
    87601 tcgtgtttcg gtttggctta cagcagtcat gcattactcc agaatgcagc gcaaggcgag
    87661 gagtatcccc gtctcatctc tctggtttca gggttacggt gcgttggcag gatttaacgc
    87721 gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt gcctagaact ggtggcgatt
    87781 gtcaatgatg tgaaaaaggg aaccatcagg ttcccttttg cgttagtgcc ggaggccgca
    87841 ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc cgacctactc cggcctactg
    87901 cgaattaatc tttcagcttg cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc
    87961 tgttagacat aacagtggtc agttcgcgat cggtcgtttc ggtcacgatg ttcagacccg
    88021 cagcctgctc gtccagctct tcaatgttga tgctcggggc gataaagccg tgttccagca
    88081 tcagcagaga gtagatagct tcctgcacac ccgcagcgcc cagtgagtga ccggtcatgg
    88141 ctttggttgc agaaatcgcc gggctcttat cgccgaacac ttcacggata gctgccagct
    88201 ctttcacgtc gccaaccgga gtcgaagtac cgtgagagtt caggtaatcg attggagtat
    88261 caacgccatg catcgccatc tgcatacaac gtactgcgcc ttcgccagac ggagcaacca
    88321 tgtctgcacc atcagaggtt gcgccgtagc caacgatttc agcatagatg taagcaccac
    88381 gcgccagcgc gtgttccagc tcttcaacca ctaccatacc gccgccgcca gcgataacga
    88441 aaccgtcacg gtgagcgtcg taagtacggg aggctttttc cggggtgtcg ttgtatttag
    88501 tagacagcgc acccatcgcg tcgaattcgc aggccatttc ccagcacagc tcttcgccgc
    88561 cgccagcaaa cacgatgtcc tgtttgccca gttggatctg ctctactgcg ttaccgatac
    88621 agtgtgcgga agtcgcacac gcggagctga tggagtagtt aacgccatga attttaaacg
    88681 gcgtggcgag acaggcagaa acgccggatg ccatcgcttt agtgaccaca tacgggccaa
    88741 ccgctttcag gccgcgcggg ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg
    88801 aaccgccgcc ggaacctgca atcaggccaa cgcgcgggtt attctggtaa gcttccggag
    88861 agaggcccgc atcagcgatt gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc
    88921 tcataaagcg cacaactttg cggtcaatga ggccagtggt atccagtttt atgttgcccc
    88981 agacgtggct acgcatgccg gaatccttca gctcctgaga gaaagtgatc cctgaacgtc
    89041 cttcacgcag agatgccagg acttcctgct ggttattacc gatgctggaa acaatgccca
    89101 ggccagtaat cactgcacgt ttcattcaat acctctgtaa gtcgcacata gagtaagttt
    89161 cgaatgcaca atagcgtaca cttgtacgcc gaacaagtcc gatcagccat ttaatagaga
    89221 aatttgcgca gccttacaca catcgctaag atcgagccac cgcctgtaag acgagtaact
    89281 tacgtgaaac actactccat acaacctgcc aacctcgaat ttaatgctga gggtacacct
    89341 gtttcccgag attttgacga tgtctatttt tccaacgata acgggctgga agagacgcgt
    89401 tatgtttttc tgggaggtaa ccaattagag gtacgctttc ctgagcatcc acatcctctg
    89461 tttgtggtag cagagagcgg cttcggcacc ggattaaact tcctgacgct atggcaggca
    89521 tttgatcagt ttcgcgaagc gcatccgcaa gcgcaattac aacgcttaca tttcattagt
    89581 tttgagaaat ttcccctcac ccgtgcggat ttagccttag cgcatcaaca ctggccggaa
    89641 ctggctccgt gggcagaaca acttcaggcg cagtggccaa tgcccttgcc cggttgccat
    89701 cgtttattgc tcgatgaagg ccgcgtgacg ctggatttat ggtttggcga tattaacgaa
    89761 ctgaccagcc aactggacga ttcgctaaat caaaaagtag atgcctggtt tctggacggc
    89821 tttgcgccag ctaaaaaccc ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg
    89881 ttggcgcgtc caggcggcac gctggcgaca tttacgtctg ccggttttgt ccgccgcggt
    89941 ttgcaggaag ccggattcac gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg
    90001 ctttgtgggg tgatggaaca gacattacca ctcccctgct ccacgccgtg gtttaatcgt
    90061 acgggcagca gcaaacggga agtagcgatt atcggcggtg gtattgccag cgcgttgttg
    90121 tcgctggcgt tattacggcg cggctggcag gtagcgcttt attgcgcgga tgaagccccc
    90181 gcgctgggtg cttccggcaa tcgccagggg gcgctgtatc cgttattaag caaacacgat
    90241 gaggcgctaa accgcttttt ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa
    90301 ttacccgtta aatttgatca tgactggtgc ggcgtcacgc agttaggctg ggatgagaaa
    90361 agccagcata aaatcgcaca gatgttgtca atggatttac ccgcagaact ggctgtagcc
    90421 gttgaggcaa atgcggttga acaaattacg ggcgttgcga caaattgcag cggcattact
    90481 tatccgcaag gtggttggct gtgcccagca gaactgaccc gtaatgtgct ggaactggcg
    90541 caacagcagg gtttgcagat ttattatcaa tatcagttac agaatttatc ccgtaaggat
    90601 gactgttggt tgttgaattt tgcaggagat cagcaagcaa cacacagcgt agtggtactg
    90661 gcgaacgggc atcaaatcag ccgtttcagc caaacgtcga ctctcccggt gtattcggtt
    90721 gccgggcagg tcagccatat tccgacaacg ccggaactgg cagagctgaa gcaggtgctg
    90781 tgctatgacg gttatctcac gccacaaaat ccggcgaatc aacatcattg tattggtgcc
    90841 agttatcatc gcggcagcga agatacggcg tacagtgagg acgatcagca gcagaatcgc
    90901 cagcggttga ttgattgttt cccgcaggca cagtgggcaa aagaggttga tgtcagtgat
    90961 aaagaggcgc gctgcggtgt gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat
    91021 gttcccgatt atgaggcaac actcgtggaa tatgcgtcgt tggcggagca aaaagatgag
    91081 gcggtaagcg cgccggtttt tgacgatctc tttatgtttg cggctttagg ttctcgcggt
    91141 ttgtgttctg ccccgctgtg tgccgagatt ctggcggcgc agatgagcga cgaaccgatt
    91201 ccgatggatg ccagtacgct ggcggcgtta aacccgaatc ggttatgggt gcggaaattg
    91261 ttgaagggta aagcggttaa ggcgcggtaa tctgctctcg cagtgtttgc cggatgcggc
    91321 gtgaacgcct tatccaacct acgtgtgacg tgtaggcctg ataagacgcg ataagcgtcg
    91381 catcaggcat ggtgctccag acgccgcaac ttacagctgt gacgcctgct gaaacaggtt
    91441 ttcccacata tcggtcacca gcgcctggtc acgcggcgac aattcaccgg caccaatggc
    91501 tttttccaga ctctggctaa cggtcgtatg caccgcctga gcggagtggt catcaccact
    91561 ttccagttct gcgatggcta atgtcaggtg gccacgcaaa tacccactgg caaacaactc
    91621 atcatcactg gcatggtcaa ccatgccgtc gattaatgcc agaatgcgtg attcaaactc
    91681 cgcgatcatc ttctttcctc attgtaaaac gtggcgctgg cttcagttga gcgcttccgg
    91741 ccacgggaac tgttccgccg taagttccgg cgtgtgataa taattttgta gtgctttaat
    91801 gaagcgtgcc gggcgttccg gaatgccatt tgtcagataa tccatcacct gcgcatgtac
    91861 ccgccgttga aacaccacac gatccggttc gaaatcccct tccagattgt cgcaactgac
    91921 gttaaacgga tatccagccg ccacgcagaa taaccagtcg aacgcctgcg gcttcacttc
    91981 aacatcttca aactggcttt gcgtctgggc atcgcgtccg tccgggcaat accagtagcc
    92041 gaaatcaacc agctcacggc gcgcttttcc ggcaatacac cagtgcgaaa tctcatgaat
    92101 tgcgctggca taaaagccat gagcaaagac gattcggtta tacggtactt ccgcatcagc
    92161 aggaagatag atcggttcgt cgtcgccttt aatcagacgg gtattaaatt catcggcaaa
    92221 gcagctatta aaaatttcaa tcaactgctc gtagtggtgt gtactgttca ttagttcatc
    92281 cccaaccagt ggaggatctc ctgtccgtgg ctatcgtaaa gtaatttggc actcatcacc
    92341 gccgagacga taacaatcat cgggcggatc agcttttgtc ctttgctcaa caccaaccgt
    92401 gaacccatgc gcgcgccgag gaactgcccc accagcatga caaagcccgt cgcccaaatc
    92461 actttgccgc cgagaataaa cagcagcaga ccgccaatgt ttgacgttgc gttgagtaat
    92521 ttggcgtgag ccgtggcttt ggcgaggttg aatccgcaca gcataacgaa ggccagcgcg
    92581 taaaacgatc cggcagccgg gccaaagaat ccgtcataaa aaccgacgca gccaccggca
    92641 attaacgcaa agggtaaacc gtacatccgg cgctggcgat cttcttcacc cagctttggc
    92701 atcagcaaga aatagaggcc gatacaaatc accagaatgg gcaaaatctg ccgtaagaca
    92761 tcagcctgaa cgtactgtac caacagtgcg ccgctcattg agccgacaaa ggtcatggca
    92821 atattgagtt tctgatcgct taaactaacc actttgcggc gaataaagta gatagtggcg
    92881 gaaatagagc cgccgcaggc ttgcagttta ttggttgcca gcgcgttagc gggagacatt
    92941 cccgcagcca tcaatgccgg aatggtgagt aacccaccgc caccggcaat cgagtcgata
    93001 aatcccgcca gcatggcgac aaaaaagaga actcccagca acagcggaga aaccataaac
    93061 aggctattaa acgtttccat tagatcacgt gctcatccag tagcgcctgg caggaaggcg
    93121 gcaacggagg cggtgtcttc ttctcaggct ttgttgttcc cggttttgga ggttcaaacc
    93181 agctttgcag ttctgccccg caaccatcgc ctgatggcgg taaaggttga tcttcacact
    93241 ccagactatc ggcagggcaa cgtaatcgta catgcatatg cgcgcgatgc tggaaccagg
    93301 gtcgcacttt gcgcaaccag tcgcgatcgg tgcccgcatc aaggcaaagt tgttgtttaa
    93361 tcgccggatt aacaaaaatg cgcgtgacgt ctttgtcctg ggcggcgagt ttgatcaagc
    93421 tgaaaatttc tggcttccac agcgtggaga caacgtgttt accgtcgcgg gaaactaagt
    93481 ccagtgcttg cgggcgcaag agctgcgcgg aggtccagcg agttttcggc agttgcagaa
    93541 agatatcgac atccagtccg gtctggtggc tggcatgacc gccgttgaaa cgcccaccag
    93601 cgggcatccc catatcgcca atcagcaccg tacccatgcc cagattgctc acctggctac
    93661 tcagacgctg gataaacatc accagatccg ggtgaccgaa atagcgacgc tgatcggtac
    93721 gcatgacctg ataatgttcg gactgtatcg gcaacgtgtc agcgccgaca atacagccat
    93781 tagaaaaact gcctatcgat tgtgcgctac ccggcacagg ttgggttatt ttttgccacg
    93841 gcgtcgctgc caggctagcg ctactggcaa gcagagccag cagcgcaatc gcggttttgt
    93901 tcatttttta ccagcgtgga atatcagtct tcacatcggc attttgcgcc cgctgccgta
    93961 acaggtgatc cattaaaacg atcgccagca tcgcttcagc gatcggcact gcacggatcc
    94021 cgacacaggg atcgtgacgg cctttggtga tcatctcaac ttcttcgcca aagcggttaa
    94081 tagtgcgccc cggcacggta atgctggagg tcggtttcag cgccatatga gcaatgattt
    94141 gctgcccgct gctgataccg ccgaaaatgc cgcccgcatg gttgctctgg aaaccctctt
    94201 tggtgatttc gtcgcggttc tggctgccgc gcagcgccac tacgtcaaaa ccgtcgccaa
    94261 tttccacgcc tttcaccgcg ttgatgctca tcagcgcatg ggcgatgtcg gcatccaggc
    94321 ggtcaaagac cggctcgcca agtccggcgg ggacaccact ggcaacaacg gtgactttcg
    94381 cgccgatgga gtcgccctct tttttcagcg cgcgcatcag ttcatctaac gcgtcgattt
    94441 tgtcggggtc cgggcagaag aacggatttt gttcaaccag cgaccagtct ttgatttcca
    94501 gcggaatgtc gcccatctgg gtcaggcagc cgcggatttc aataccaaat ttctcggcga
    94561 gatatttttt ggcaatcgcc cctgccgcca cgcgcatggc ggtttcgcgg gcggaagaac
    94621 gtccaccgcc gcgataatcg cgcagaccgt atttttgttc gtaggtgtaa tcagcatggc
    94681 ctggacggaa gacgtcttta attgcgctgt aatcctgaga acgctggtcg gtattttcga
    94741 tcaacaagcc aatgctggtg ccagtagtaa cgccttcaaa aacaccggag agtattttga
    94801 cctgatccgg ctcgcggcgc tgggtggtat agcgcgatgt cccagggcga cgacgatcaa
    94861 ggtcatgttg caggtccgct tccgtcagcg gaatgcctgg cggaacacca tcgacgatgc
    94921 agccgagcgc cagcccgtgc gattcgccga aggtggttac gcgaaagagt tgtccaattg
    94981 tgtttccagc catcacggct ccgttattgt tgtgtttgcg tgtttactta atctttataa
    95041 atcgcgaaat gttctcgtgc ggcaagaagc tgctctttag tgagcataaa cacaccatcg
    95101 ccgccgttat caaactccag ccaggtgaac ggaacatcgg gatattgttc cataagatgt
    95161 accatgctgt tgcccacttc acaaatcaac acgccatcat cagcaaggta atctgccgcg
    95221 ttaccgagaa tgcgacgcgt cagtttcagg ccgtcggtgc cagatgccag gcccagttcc
    95281 ggctcgtgac ggtattcgtt tggcaggtcg gacatatctt ccgcgtcgac atacggcggg
    95341 ttagtgacaa tcagatcgta ttgcactttc ggcaaatcac ggaacagatc ggaacgaatc
    95401 ggaatgacgt tgtggatcag accgtgttct tcgatgttct gttcggcaac cgccagcgcg
    95461 tcgggagaga tatctaccgc gtcgacttcc gcttccggga aggcataagc acaggcaatg
    95521 gcgatgcagc cgctaccggt acacatatct aaaatatgct gcggttgctt gctgatgagt
    95581 ccggcaaatt tattgttgat cagttcacca atcggcgagc gcggcaccag cacgcgttca
    95641 tcgacgtaaa attcatgacc gcagaaccag gctttgttgg tcaggtaagc caccggaatg
    95701 cgctcgttga cgcggcggat cacgcgttca acaatacggt gtttttcgct ggaggtcaga
    95761 cgcgcggtgc gcatatcttc cgggatatcc agcggcaggt agagcgatgg taacaccagc
    95821 tgtacggctt catcccacgg gttatcggtg ccgtgaccgt accagatatt tgccgcgctg
    95881 aagcggctta ccgaccagcg caacatgtcc tgaatggttt gcagctcatt tactgcttca
    95941 tcaacgaaaa ttttatccac gtattcctcc agggcatgat cgcaataatt tcggcggcta
    96001 gtttgccatg aagatgacga taaatcagca ttcacgcgcg gtgagtgagg aaaaatacgt
    96061 ttaaaacgat cgattgtgct acgagtcggg taaactgtag gaaaattaga aatagagaca
    96121 gataaatgaa aaagaaaaca acacttagcg aggaggacca ggctctgttt cgccagttga
    96181 tggcggggac tcgcaagatt aagcaggaca cgattgtcca ccgaccgcag cgtaaaaaaa
    96241 tcagcgaagt gccggtgaaa cgcttgatcc aggagcaggc tgatgccagc cattatttct
    96301 ccgatgagtt tcagccgtta ttaaataccg aaggtccggt gaaatatgtt cgcccggatg
    96361 tcagccattt tgaggcgaag aaactgcgcc gcggcgatta ttcaccggaa ttgtttttgg
    96421 atttacacgg tctgacgcag ctgcaggcca agcaggaact gggcgcgttg attgccgcct
    96481 gtcgccgcga acatgtgttt tgtgcctgcg tgatgcatgg gcacgggaag catattttga
    96541 agcaacaaac accgctgtgg ctggcacaac atccgcatgt gatggcattt caccaggcac
    96601 cgaaagagta cggcggtgat gcggctttgt tggtgttgat tgaagtggaa gagtggttgc
    96661 cgccggagtt gccctgaatg ctggcagcaa atgccggagc agcgtaaacg ccttatccgg
    96721 cctacaaata tgtgataatt caataaattg caattcactt cgtaggcctg ataagacgca
    96781 tcagagcaat actacgaact tcagttcatt caatacatac tgaaaacgac acgtcgaatc
    96841 aatcggttgc ccactactgg tattcacatt caccacaata tcgtgtagta ctttatccgt
    96901 taccggatga cgagttggcg taaccgttac cgttgattta tcacaaccgt aagaggtaaa
    96961 ggttaacaat tctgataccg cgcggcagta tgctcctctg ccatcattat cagaaaaatt
    97021 actgccatca cagagtctgc cgccttccat caggtaggtc gaaagcgtgg tggtcactgt
    97081 tgaccccgtg gactgaattc gtaacgcctt attatggctg acataatttt cactccagga
    97141 gatatcgtat gaagcgaccg gtatcgttgg gcatattgtg ttatgcactg taggggccgt
    97201 atagcttcca aatccgggta aatctgtata ggaataaaag gatgcgatcc aagggcaacc
    97261 ataatcatca gatgcaacgg taattttaaa cgtcatatat gcacaacgaa aaattttcgt
    97321 atctatgcca gggcaatttg agttattaat tgtatcaaag tcagtcagta ccaaagttgg
    97381 agctgaaccc gacccagacc aggaaataac ccgcaacgta tcgcctttat acgcaaaaga
    97441 atcatatttc ccatatgcaa cctcctttgg cgttatcacc gtaacattga aaacactgct
    97501 cgctacagca ccggttacct tgtccatggc ataataaact ctcacactgt caaaactcgc
    97561 actcataagt ttatctccag cataactctt agatgcgccg acaacactca gaacgagcag
    97621 cattaggctc gcgaaaatta tggtccattt tttcatttca gaaatactca aaggttgccg
    97681 tcactgtggc actaaagtct ccggacgtta catctcgccc gctctttgcc tgtaactagg
    97741 tattaaaatt taggctgtta tttccctgat tcagcgggag cgtgacgcct gttgtattgt
    97801 tgattggcac cgatgtgcct gccgcagttt caataccaat cccagcgccg gatgctgatg
    97861 acgaggagtc aaaggccaga aagccgggta gagcactatc ttctgttcct gtcaacgtta
    97921 ctttgacatt aaacaatgta gaactatgac aatctttcaa ttgaaacacc aatggcactc
    97981 gctctgactg cccaaggttc attaaatcgc tggcggcaat ggcaggaaaa tgtacttctg
    98041 ccaactggct accattaatc actggcgtac acgatttact gattaaattg ccggtaaagt
    98101 gcaaattatc tcctgctatt gtggttccac tgcccagcaa catcaggcta ctaataaaaa
    98161 acaacgtacg ctttttcttc attggtaatc tacctgtaac gtggcccagg cctcaaagtc
    98221 cgcctctggc agaatgacac cactcttttt tactggcaca gccgttaaca ccggtaaatc
    98281 ggtttcattc accacaagtg gcgtattgat cgtaaaactc tgccctgcct gtttgagctg
    98341 aatgcctaaa ccagtgatat tgctgcttac tgcagcatta tcaaaatcgc ttggtgtgcc
    98401 actccagctc aaggtcattt ccattaatgg gtcgcgaacc gccgtatcgc aggtaatgga
    98461 atacggcacg gtttgctcat aattgctgcc attaatgtca tcaataagca catcctgaaa
    98521 agttatttca atggtttgag cgttgctaat cgtgcagtta ggcggctgca ctaaataacc
    98581 gtgaaaagag acgtttgaga gtgccatcga gcaaaagccc cacagcaaaa taagcgagat
    98641 ccgtttcatt ggtaatccac cacaattgtg gcactggcgt taaattcagc tgccgctaac
    98701 gttgtaccac tctgtttcac cggcacagct tccagaacgg gaactcccga ggaaaaatta
    98761 aatggcaacc atgagccaga ggttaagtcg agtattgtgt gatcactact tttctgaacc
    98821 cgaataccaa acccaggaat acccgtgctg ataactgttt caccgttaat cacgaccgtt
    98881 gtcgcctgca tctgtagacg caggtcatcc attgccagat tgttacaaac aagcgagtag
    98941 tcaatgggac gtttataatc aaccctattg atcttattaa tgaatacatt accaaactca
    99001 actgccgcac catttaccgt acaaggagga ggggcatcga caagcacccg taaattcata
    99061 cttactgact tactcgctgc aactgcaaag ccgctaccaa cacaacatat cagtaaccca
    99121 gttaccgccc agacagaaca agatcctttc atactggctc tcccggcctc agcctttgcg
    99181 gctctgctct tcatcgacct agcaactgtt accgttgcaa gtgaaaaaga gcggcatccg
    99241 agcgccatag tcgttgacat aggtcagtac cggcacgcta cccatcttca cattcagcgg
    99301 tgcagaggtt ttcggcgaca tcactattgg cgagaaaccg gctgccggat tgccattttt
    99361 ctgtgtgctg gcattactga aaatgacgta atacgacgtc gggttattga cggtaaattc
    99421 ctgaccgttg cgggttaaag taaccttata ttgatagggg tttttcatgc tgacgttctc
    99481 cagcgccttc ggtcgccaga acaacttaat acgcgtttgt agtgctattt gcagcgtgtt
    99541 ggctttgttt gattttggcg ggatctcacg cacgttgaag tagaacaggc tttcacgatc
    99601 ggcaggcaat ttgttgatat ccggcattcc ctgcactttt acctgcccgt tcatcattga
    99661 atcgatgcgt tgcaccggtg gcagaacagt taacggtgaa ctgatcttgt tacccttttc
    99721 atcttcgatc cagctttggg ccagataagg cagtttcgga tcgttattgc gcaacgtcac
    99781 gctgattgat ttgtcgcttt cattaaatat caaacgggta cgatctggcg ttacagaagc
    99841 ctgggcagac aaactggcag cgctaagcag taacgctagc gtcgtagcac ccaatttggc
    99901 agagcaaagt aaatcagaca tggatcattc cttaatcacg ttattggtta gctgaaacag
    99961 aaacaggggg attggtgggt gtcacctgtt gcgtctgctc ctgaatgaca ggctttatgt
   100021 catcaggaac aaccggcgcg acgttgcctt tatgctggca tggcagtaac aagccgttaa
   100081 acagatcggc aggcagcggg tccggcaggt tgatatcgca atgcgcaaca ccactccaga
   100141 acacactcat gtgttcgcca ggtttcaccc cagccagata aacattgccg tcatcatcga
   100201 caaggcccac tgtctgctcg ttatcatttt ttacttctgc gccaaacgga ggatggctgc
   100261 cgtcttgtaa acgcagcacc gccatcgctt tttgaccact gatgacggca aatttgcggt
   100321 agccaatcgc cccttcagtt agcgtggctt gtaccaccga ctgggttgct tcggcgtttt
   100381 ccggcagttt gttgaggtcg atatacgcct gattgcggta atagttattg acgtcagaaa
   100441 cgacggcttt accaaacata ttggtataaa cagcagcacc gttaccttct accggaacat
   100501 cggcaacgcc atcggcatca atcaacaagc gtgtcccgcc catattctgg gtacggtgaa
   100561 gtgcgccacc gtgggcagta aacgttgcgc cgccctgtaa cgacaagccc gcagaggtgt
   100621 attgcccttc atggtagttc gcactgaggt caacctgcgc cagcgaacca tcatggctgt
   100681 aatagccgtc aacgctggtg tgtttgtcac tggtgccgac gttcaactga tagtgagtcg
   100741 cgtcatcgac acggctgaaa taaccgacct gactgctgtc cgtcccactg ccatagtacc
   100801 gttatagcta acggtactgt tgtcgcccca cggcatactg agcgaaatgt acatgccttt
   100861 gtcggcctgg ttgtcatact cgtaacggta gccagtcatc gagatgctga cattacgaat
   100921 actgcccata ttgaaatagt gcgagagcat gatgttgtag tttgtctgct cctcgcgatc
   100981 ccagtaggta tggcgggtat agttgagata aaccgaaaca cccgcatcgc ggaagttctg
   101041 gttataagtg gcggtgtaca tctctttgtc gttgcccgtg cgtaccattc cgctgtcact
   101101 ggcatccaga tactcgctca tggtcataaa gttctcttcc gagaagcgat atccagcaaa
   101161 agtaacgcgg ctgttgagct ggtcaaagtc tttggaataa ctcacacgga aggagttacc
   101221 gtccagcgaa cctttgccat aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac
   101281 atcaaacgcg accgcgccga atgtagacaa atcgcgaccg acaccaagcg ccgcagactg
   101341 atagttttca tctcccagtg cgccgccata taacgaccag ccgttagcga tcccccagga
   101401 agcttcagca ccagaaaaaa attcaccctc gacatggtgt ccccactctt gcggacggcc
   101461 catcatgatc ttatagcgaa cctgacctgg acgagtgagg tatggcatcg aggcggtgct
   101521 gatgtcatat tcctgcacct ggccgttctg ttcttcaatg cgaatatgca acgtaccgga
   101581 gacagaatca ccaagatcct gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat
   101641 cacacgcccc atctggctga cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc
   101701 tggcgcgtag ccgcgcagat tgggaggcaa catttgatcg tcagtactga cactgccacc
   101761 aacatagtta aaaccatcaa aaatatcgga tctgaggtaa tcctcgccca gcgccagttt
   101821 ggcttttaat gatggtaacg cccgcctggc atagtagcga ctccactccc attttttttg
   101881 agtttcatcg ccgctgaatt catcgtcatc attactgcga gtatgttgat agttagtctg
   101941 ccagtcagca cgcatacgcc acggccctag gttaacccca accgtcccgt tgccgctgat
   102001 ctcgttacta tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga tgctgtagtc
   102061 cgcgacgatc ccggagatgc cgtcatccca acgtgaaggc ggatcccagt cgggataagt
   102121 atattcgagg taagcctgtg gcagtgaaat gactaatgcg gactggctta aatcagcctt
   102181 aatttccatg ccttccagtt gaccggattt caggcattta gcatcgtggc tccattgcag
   102241 atttttcgcc acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc
   102301 atagcttttg ctcgcgtcat cttcaccagc ataccagtaa atatcgtact cttccgccaa
   102361 tggctgttta tttagttgaa cctgtaaatt gtattttccg ggctcaacat atccctggct
   102421 ggaaaaacgc ttcagatcaa tttttgtgtc gccttttaat tccagaaaac gggaatcaaa
   102481 ctgaatgtcg tcttcagccc agacactgct ataactccct gacattgcca gcgcaatgca
   102541 ccacggaaga atccgtaatc gaaaaagaga atggtcaggc atacacgttt ctatccgttt
   102601 ttattcttgt tcggtcaaaa tccatttaaa tcccccatag cagtgtcgct atgggggtat
   102661 gccttacagg taagtaatct ggaaggtggt atttgcttca aaattaccca gatctggcgc
   102721 atcagctgcg ccgaccagcc aggctttaaa gttcagtgtc tgcttcgctt tgcctttgtt
   102781 ggtagaagtg tctttcacta ttttctggtc aatacccatg ccgcttttgt aagaggtacc
   102841 cagagagtca ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat cggtattgaa
   102901 aatggtgtaa taattgccgc tatttgcaga agaaacggta ccagtaaagg tagtggtcat
   102961 tgtttcctga gtatcaaata cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt
   103021 ggtgccgtta tttttcagtt tttcggcacc gatatcaccc atatcaactt ccagtttgtg
   103081 atcgtccgga acgatagaac acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc
   103141 gttgttaccc gcagcgattg ttgccgtaga ggtgaacacg cccatcacca ttgccgcagc
   103201 aatagctgtt ttaacaaact tactcattat ttgtccttaa actataaata attaaaaata
   103261 taaacattgc aatacattga ttcagtcaat agccaatgtt tatacccagc cattgaatac
   103321 ggcatgcgac ccaaaaagaa caggtcgtag aaaattgtat tcattggtgt ggggccgcat
   103381 agtgatgttt ccacacccct gatccaattt aattttacta aagagtaaat gtagatttaa
   103441 ttaatatatt gacagaagaa aggttatctt aaggacatcc ttaaaaaata cggaataagt
   103501 atttagaaaa aatagaaagt atatcaataa tgaatatcaa cagcttaaaa aacaaacaat
   103561 gaacatataa tgcgattggc atttatccac ttttgacaat ataatatata aacactatag
   103621 ccagaattta atcaagtttc aattacatcc tgcctgatta ttcaactcta ttttctctat
   103681 tatctgtatt aataaatgac actgagtgtc aaacagacat tattcatttc aacatgtaac
   103741 tttgggcaaa atcaagatac gcgcagaaga ataatttccg aatgaatgta gaaagtgttt
   103801 tatttataga taaggagagt cttcgatagt agtgaggttg acagatatcc gctgcctaat
   103861 gagcaattat gcccaatgaa tctacctcat tgggcataat ttggaacaca tcagatagct
   103921 tttgccatct tcagattaca cgggctcatc tgccagttga atgtaccgtt tccgctttca
   103981 tcaagcgtta cgctggcaat ggccgaggtg gtaaacatag gcggcgtttc gcccggacat
   104041 aactcggcaa ccagataccc gactaacggt aagtgagaga tcaccagtac cgaagcaaca
   104101 ccttcattgg tcaacgcctg caaataggca ctgaccagac caacatcgcc gcagggcgtt
   104161 aactccggca gaacttctgc actggaaggc aggttcagac aatcccctac ttcttccagt
   104221 gtttgctcgg ctcgcaggaa cgggctcacc agaacgcgtt cgatttccac tttttgacct
   104281 ttcagccagt tcgccatcag gcgagattcg tcacaaccat tagtggtcag aggacgaacc
   104341 gaatcactgg cggcatcgag ggctgcgtcg ccgtgacgca tgataaaaac ttgcatattg
   104401 caccgctttt gttaaccaga ttcgcccgtt ttctttcatc tgaaaccaga gaaagaaaac
   104461 ggtggccggc attgtgcctt atccattcac cgaatgaaac gctgtttttt acctcaatgg
   104521 cgtaagtata gtcaatatgc gtttacattt tgaccaacac tccgccattc agcgcggatt
   104581 catatagctt tgaccttctt attgcaggtc agttgcagtt gttttccaaa aactttcccc
   104641 acgcgcgccc atctcgacca aacgctcgca aggggtaaaa cgggaaccat actgcgtggc
   104701 cagtcgttgc attattgcaa ccacttcgcc cgcgccgaga gaatcgatat agcggaacgg
   104761 tccaccgaga aatggcggaa aaccaatgcc aaataccgca ccaatatccc cgtcacgcac
   104821 gctacggata acctgctcat caacacaacg tactgcttca ttcagcatca acatcacaca
   104881 ccgttcagca acctgcggtg cggagagtcg cccctgccct tgtgcgccaa tgagcgggta
   104941 aatggcagga tcgacctgtt ttttgctttt acgccctttc tgaccataaa gatagaaacc
   105001 ccgaccattt tttctgcctt tgcgatcgtc gttcaaaatt gaagaaacaa catttgcagg
   105061 cgcgctaaaa cgttctccat aagcggcttc cagtacagga ataattttag tcccggtgtc
   105121 gattcctacc tcatccaaaa gttggattgg gcctaccgga aaaccaaatt tcactagcgc
   105181 ggcatcaatg tgctctaccc gttcaccttc ggtcagcatg cggatagctt cattaatgta
   105241 aggcgctaag atgcgattga cgtaaaaccc ggctttgtca cgcacgacaa tgggcgtttt
   105301 gccctgtttt ttcgccagtt ttaccgtagt ggcgatggtt tgcgccgatg tacccgcatg
   105361 aggaataatc tctaccagcg gcattttttc caccggactg aagaaatgca ggccaataac
   105421 ttgctcaggt cgcgcggcgt gagcggcgat atcaccaatc ggtaaagatg acgtgttcga
   105481 agcaaaaatg gtatgagcgg cgcaattttg ctccacttcc gccaccatct gttgttttaa
   105541 ttcgagattt tcaaacaccg cttcaataat cagatcgcga tgggcaaagc cgcgatagtc
   105601 cgtcgttccg gagattaatg ccagctgttt gtcacgttcg ctggctttga gatgacgacg
   105661 gcgaactttg ccctccagct gatcccaact gtacttcagc gcatgattta tgccctgcgg
   105721 gttgatatct ttaattctga ccggaagccc cgctttacaa gcagtgacat aagcaatacc
   105781 gccgcccatc aagccaccac ctaaaattcc cacgctgttt aatggcgcag gcggtgcatc
   105841 actaccggga tctttcttca cgtccgtact ggcaaaaaag atactacgca aagcttgcga
   105901 ttgcggcgtc atcgccagtt cgccaaaggc gcgagcttca gcgtcatagc cgctgctggt
   105961 gccctgcgct aatcccgttt caacaacctc caggatgcgt tctgtcgccg gataattgcc
   106021 ttgagttttg tgttctgttt tcttgccgac cattttgaac agcagcgcac gacctaacgg
   106081 ccccgccaga atacgctcgc gtacaggtag agggcgggaa gatgggcgat ccttcttcgc
   106141 cagctcaaca gcggcttcca gcaaaatgga gtgcggaaca acgtcatcca ccagccccag
   106201 ctttaatgcc tgtttcgccc gaagttgttt tccggtgaga atcatctcta atgctgtact
   106261 gacgcctatc agacgcggta aacgctgggt gccgcctgaa ccgggtaaca atccaagttg
   106321 tacttcaggc aaaccgagca ccgttttggg atcgtcagta caaacgcgac cgtggcacgc
   106381 cagcgccagc tccagcccac cgcccaggca agcgccatga atagccgcga taaccggaat
   106441 gggcaaagca tgaatctccg ccatcaactg ttggccctgc cgcgccagtg cttccgcttc
   106501 ttgcgccgtt ttgcagttgc caatcatgtt gatgtctgct ccggcgataa aattgtccgg
   106561 tttcgcggag ataaacacca cgccgcgcaa ctctttgttt tcacggagtt gcttaataat
   106621 ggcgcgcacc tgcgaggcaa attccgcctt cagggtattc attttctcat ccggtacgtc
   106681 gatggtgatg atagcaatgt tgtccagacg aacattaaga gtaaacgctg atgccatttc
   106741 cattattccg cctccagaac cattgccgcg ccaagcccac cggcagcaca ggcggtaact
   106801 aaaccaaatc caccgccgcg acggcgaagt tcatgcaatg tctgggtaat catccgcgcg
   106861 ccggtcgccg cgaagggatg tccgtaagca atcgaaccgc caagcacgtt aaatttgcta
   106921 tcgtccactt cgccagtggc atgtgcacgc cccagtactt cacgagcaaa acgttcacta
   106981 cccagcaact gaatattcgc cagtgtctga gctgcaaagg cttcgtgcat atcgatcaat
   107041 gtcagttcgc tcatcgtcaa accggcacgt tccagcgcca gcggtgttga ccaggctgga
   107101 ccgagcaaca tgtcctgcca gacatcaatc gcagtaaatg cgtagctgcg cagatacccc
   107161 agcggcacca gccctaattc tttcgcccgg gattcagtca tcaggatcac cgctgccgcg
   107221 ccatcggtca gcggcgtact gtttgccgcc gttaccgttc cgtgtttgcg atcaaacgcc
   107281 gggcgcagct ttgcgtaatc ggcaagcgag gaattaccgc gaatattgtt gtcttcgaca
   107341 agcggttgtt tataaggagg gataaaggca gtcatcacct cttctttgag ttttccgtct
   107401 gaccatgcct gagcggcacg ctgatgcgaa cggtgcgcta atgcatcttg ctgttctcgg
   107461 gtgatgccgt aggttttcgc catttgttcg gcggtgtcgc ccatccgcaa gccagtagaa
   107521 tattctgcta ccgcaggcgg tacgggcatt aagtcgcgca aacgcaggcg agagaagagt
   107581 ttcaggcgct ggctcatggt tcgagctttg ttgacatcaa ccagcacgtg cgccagtttt
   107641 ttactgacgc caattggcaa tacggaagag gaatctgccc caccggcaat ccccgctcgg
   107701 atagttcccg ccatcaggct ttctgcgacg tttgcaaccg cctggaaact ggtagcgcaa
   107761 gcgcggctga cgctgtaagc atcggtatgc acattcattc cggtaccgag aacaatttca
   107821 cgcgcaatgt tgggggcttc aggcatttgt acgacctgac caaagaccag ctgttcaatc
   107881 acttcagcgg ggatctcgct gcgtgccagc agttcgccta ccaccatctt ccctaaatca
   107941 accgcgggaa tgccatgaaa agccgtcgcc tggcgggcaa aaggcgtacg taaaccgcta
   108001 acaatggcga tacgatcgcc ctggcgggta accagtggta aaacctgacc cataacactc
   108061 ccctgtaaaa aaataaataa agtggtctga cctgatcata gtattaacca tttttttaca
   108121 tttagccaag cggagaaaaa ggaaagtggg agctatgaca cagtgaagac agaagaaaga
   108181 aaagaaaacg cccctgccag caactgacag gggcgtacgt acccgagagg aattaacgca
   108241 gacccagctg gaagatcagc atttcagctt cgcaggcaaa agtgaaatcg atatccaggc
   108301 gcacaccgtc agactcttca gtgaaagtcg gggagatttt gcacggttcg gattccacgc
   108361 tacgggattt ttcagtcagc gccgccagcg tttgttctgc ttctgcgcgg tttgcaaaca
   108421 cgcggctgta agacgcggtg cagtcggagt tgtccataat ggtgccaaca tccatacagc
   108481 agcaaaccgg ggtttcatca gcactacatt tactcatcgt tgatttcctc tgtatgtgca
   108541 cccaaggtgc cagataaacg ttgtggatat tttacgcttc cggaaagtgc tgctccagtt
   108601 gttaattctg caaaatcgga taagtgaccg aaatcacact taaaaatgat ctaaaacaaa
   108661 attcacccga atccgcgagt gcgcaacctc caaattttgc cagctggatc gcgtttctta
   108721 gatcatattt gaaaaaagat ggaaacatac ttgcaacatt ctagctggtc cgacctatac
   108781 tctcgccact ggtctgattt ctaagatgta cctcagaccc tacacttcgc gctcctgtta
   108841 cagcacgtaa catagtttgt ataaaaataa atcattgagg ttatggtcat gagccagaaa
   108901 accctgttta caaagtctgc tctcgcagtc gcagtggcac ttatctccac ccaggcctgg
   108961 tcggcaggct ttcagttaaa cgaattttct tcctctggcc tgggccgggc ttattcaggg
   109021 gaaggcgcaa ttgccgatga tgcaggtaac gttagccgta accccgcatt gattaccatg
   109081 tttgaccgcc cgacattttc tgcgggtgcg gtttatattg acccggatgt gaatatcagc
   109141 ggaacgtctc cgtctggtcg tagcctgcaa gccgataaca tcgcgcctac ggcatgggtt
   109201 ccgaacatgc actttgttgc accgattaac gaccaatttg gttggggcgc ttctattacc
   109261 tctaactatg gtctggcaac agagtttaat gatacttatg caggcggctc tgtcgggggt
   109321 acaactgacc ttgaaaccat gaacctgaac ttaagcggtg cgtatcgctt aaataatgca
   109381 tggagctttg gtcttggttt caacgccatc tacgctcgcg cgaaaattga acgtttcgca
   109441 ggcgatctgg ggcagttggt tgctggtcag attatgcaat ctcctgccgg gcaaactcct
   109501 caagggcaag cattggcagc taccgccaac ggtatcgaca gtaataccaa aatcgctcat
   109561 ctgaacggta accagtgggg ctttggctgg aacgccggaa tcctgtatga actggataaa
   109621 aataaccgct atgcactgac ctaccgttct gaagtgaaaa ttgacttcaa aggtaactac
   109681 agcagcgatc ttaatcgtgc gtttaataac tacggtttgc caattcctac cgcgacaggt
   109741 ggcgcaacgc aatcgggtta tctgacgctg aacctgcctg aaatgtggga agtgtcaggt
   109801 tataaccgtg ttgatccaca gtgggcgatt cactatagcc tggcttacac cagctggagt
   109861 cagttccagc agctgaaagc gacctcaacc agtggcgaca cgctgttcca gaaacatgaa
   109921 ggctttaaag atgcttaccg catcgcgttg ggtaccactt attactacga tgataactgg
   109981 actttccgta ccggtatcgc ctttgatgac agcccagttc cggctcagaa tcgttctatc
   110041 tccattccgg accaggaccg tttctggctg agtgcaggta cgacttacgc atttaataaa
   110101 gatgcttcag tcgacgttgg tgtttcttat atgcacggtc agagcgtgaa aattaacgaa
   110161 ggcccatacc agttcgagtc tgaaggtaaa gcctggctgt tcggtactaa ctttaactac
   110221 gcgttctgat aacgcgttcg cctggataaa gtcacctgct atgcaggtga ctttaactcc
   110281 cccacttcac cgaaagtagt ttcctcattg cttgcctcct gagttttgca aacagcctga
   110341 tggcagcttg ccctttcaaa tcagtaagtg gtatccacag acaaacctga agggaaatgc
   110401 atattttcaa gcgttctgct cgtccttctc aaagagttta cttttctgca tttccaggat
   110461 actcaacccc cctggctatt gtgcgcttat acactcaaat taaagatagg ttctaaataa
   110521 atgagcgttt tttgatagtc tatttcatta ggtaatatat atttgtaaca aatcaatcaa
   110581 catagaataa aatcatgcta ccatctattt caatcaacaa taccagcgca gcttacccag
   110641 aatccatcaa tgaaaataac aatgatgaaa ttaatggatt agtacaagag ttcaaaaacc
   110701 tttttaatgg taaggaagga ataagcacct gtattaaaca tctacttgag cttataaaaa
   110761 acgccatacg agtaaacgac gatccttata gatttaatat taataattcc tcagttactt
   110821 atattgatat tggctccaat gatacagacc atattactat tggtatcgac aaccaagaac
   110881 caatagaatt acctgcgaac tataaagaca aagaactcgt ccgtactatc attaatgaca
   110941 acatagttga gaagactcat gatatcaata acaaggaaat gatcttcagc gcattaaaag
   111001 aaatatatga tggagatcct ggttttatct tcgataagat atcacacaaa ctcagacata
   111061 cggtaacgga atttgatgag agcgggaaaa gcgaaccaac ggacttattt acctggtacg
   111121 gtaaagataa aaaaggcgac tctctcgcta ttgtaattaa aaataaaaac ggaaatgatt
   111181 acttatctct cggttactac gatcaggacg actaccacat tcaaagagga attcgtatta
   111241 atggtgatag tctcacccaa tattgtagtg aaaacgccag gagtgcttca gcgtggtttg
   111301 aaagcagtaa agctatcatg gcagaatcat ttgcaactgg ttccgatcat caggttgtaa
   111361 acgagctcaa cggggaaaga ctgagagaac ctaacgaagt ttttaaacgt ttaggtcgag
   111421 caataagata taattttcaa gtggacgatg caaaatttag acgcgataat gtaaaagaaa
   111481 taatttctac tttattcgct aacaaagttg acgttgacca tcctgaaaat aaatacaaag
   111541 actttaagga tctcgaagat aaagttgaaa aaagacttca aaattgccag gcgaaatatc
   111601 aaaatgaaat taatcaacta tctgcactag gtgttaattt tgatgatatt taaaattatt
   111661 tttccagttt tttgattttt caacgcctga atatcggaaa tagtcagtga caaa
//
LOCUS       DADLTF010000002        82346 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.002, whole genome shotgun sequence.
ACCESSION   DADLTF010000002 DADLTF010000000
VERSION     DADLTF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 82346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.002"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN749_000107"
     CDS             complement(<1..209)
                     /locus_tag="JN749_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7366652.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN749_000108"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="JN749_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAY7366653.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN749_000109"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="JN749_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAY7366654.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN749_000110"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="JN749_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAY7366655.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN749_000111"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="JN749_000111"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HAY7366656.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTVQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN749_000112"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="JN749_000112"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HAY7366657.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN749_000113"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="JN749_000113"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HAY7366658.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="JN749_000114"
     CDS             complement(4598..5320)
                     /locus_tag="JN749_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7366659.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN749_000115"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="JN749_000115"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7366660.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="JN749_000116"
     CDS             6520..6738
                     /locus_tag="JN749_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HAY7366661.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN749_000117"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="JN749_000117"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7366662.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETEQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="JN749_000118"
     CDS             complement(7472..7672)
                     /locus_tag="JN749_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HAY7366663.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN749_000119"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="JN749_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HAY7366664.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN749_000120"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="JN749_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HAY7366665.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="JN749_000121"
     CDS             10169..12082
                     /locus_tag="JN749_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7366666.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="JN749_000122"
     CDS             12082..13104
                     /locus_tag="JN749_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7366667.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="JN749_000123"
     CDS             13098..13316
                     /locus_tag="JN749_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HAY7366668.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="JN749_000124"
     CDS             13370..14239
                     /locus_tag="JN749_000124"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HAY7366669.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="JN749_000125"
     CDS             complement(14294..14698)
                     /locus_tag="JN749_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HAY7366670.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="JN749_000126"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="JN749_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HAY7366671.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="JN749_000127"
     CDS             15683..17773
                     /locus_tag="JN749_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366672.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN749_000128"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="JN749_000128"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HAY7366673.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN749_000129"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="JN749_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HAY7366674.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19741..20343)
                     /locus_tag="JN749_000130"
     CDS             complement(19741..20343)
                     /locus_tag="JN749_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HAY7366675.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="JN749_000131"
     CDS             complement(20333..20500)
                     /locus_tag="JN749_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HAY7366676.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN749_000132"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="JN749_000132"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HAY7366677.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="JN749_000133"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="JN749_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HAY7366678.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /locus_tag="JN749_000134"
     CDS             22891..25434
                     /locus_tag="JN749_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HAY7366679.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKGDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="JN749_000135"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="JN749_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HAY7366680.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25883..26689
                     /gene="nirC"
                     /locus_tag="JN749_000136"
     CDS             25883..26689
                     /gene="nirC"
                     /locus_tag="JN749_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HAY7366681.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26708..28081
                     /gene="cobA"
                     /locus_tag="JN749_000137"
     CDS             26708..28081
                     /gene="cobA"
                     /locus_tag="JN749_000137"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7366682.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28328..28495
                     /locus_tag="JN749_000138"
     CDS             28328..28495
                     /locus_tag="JN749_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HAY7366683.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28790..30127
                     /gene="frlA"
                     /locus_tag="JN749_000139"
                     /pseudo
     CDS             28790..30127
                     /gene="frlA"
                     /locus_tag="JN749_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30148..31170
                     /gene="frlB"
                     /locus_tag="JN749_000140"
     CDS             30148..31170
                     /gene="frlB"
                     /locus_tag="JN749_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HAY7366684.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITLLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31220..32050
                     /gene="frlC"
                     /locus_tag="JN749_000141"
     CDS             31220..32050
                     /gene="frlC"
                     /locus_tag="JN749_000141"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HAY7366685.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIYMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32047..32832
                     /gene="frlD"
                     /locus_tag="JN749_000142"
     CDS             32047..32832
                     /gene="frlD"
                     /locus_tag="JN749_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAY7366686.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32932..33663
                     /gene="phoB"
                     /locus_tag="JN749_000143"
     CDS             32932..33663
                     /gene="phoB"
                     /locus_tag="JN749_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HAY7366687.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33823..34908)
                     /locus_tag="JN749_000144"
     CDS             complement(33823..34908)
                     /locus_tag="JN749_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7366688.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34920..36224)
                     /locus_tag="JN749_000145"
     CDS             complement(34920..36224)
                     /locus_tag="JN749_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366689.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEMALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36236..36527)
                     /locus_tag="JN749_000146"
                     /pseudo
     CDS             complement(36236..36527)
                     /locus_tag="JN749_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
     gene            complement(36538..37416)
                     /locus_tag="JN749_000147"
     CDS             complement(36538..37416)
                     /locus_tag="JN749_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HAY7366690.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37413..38639)
                     /locus_tag="JN749_000148"
     CDS             complement(37413..38639)
                     /locus_tag="JN749_000148"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7366691.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38640..39803)
                     /locus_tag="JN749_000149"
     CDS             complement(38640..39803)
                     /locus_tag="JN749_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HAY7366692.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(39887..40249)
                     /locus_tag="JN749_000150"
     CDS             complement(39887..40249)
                     /locus_tag="JN749_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAY7366693.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40266..41171)
                     /locus_tag="JN749_000151"
     CDS             complement(40266..41171)
                     /locus_tag="JN749_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366694.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41441..42445)
                     /gene="trpS"
                     /locus_tag="JN749_000152"
     CDS             complement(41441..42445)
                     /gene="trpS"
                     /locus_tag="JN749_000152"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAY7366695.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42438..43196)
                     /gene="gph"
                     /locus_tag="JN749_000153"
     CDS             complement(42438..43196)
                     /gene="gph"
                     /locus_tag="JN749_000153"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HAY7366696.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43189..43866)
                     /gene="rpe"
                     /locus_tag="JN749_000154"
     CDS             complement(43189..43866)
                     /gene="rpe"
                     /locus_tag="JN749_000154"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAY7366697.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43884..44720)
                     /gene="dam"
                     /locus_tag="JN749_000155"
     CDS             complement(43884..44720)
                     /gene="dam"
                     /locus_tag="JN749_000155"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7366698.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44827..46113)
                     /gene="damX"
                     /locus_tag="JN749_000156"
     CDS             complement(44827..46113)
                     /gene="damX"
                     /locus_tag="JN749_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HAY7366699.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46205..47293)
                     /gene="aroB"
                     /locus_tag="JN749_000157"
     CDS             complement(46205..47293)
                     /gene="aroB"
                     /locus_tag="JN749_000157"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAY7366700.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47350..47871)
                     /gene="aroK"
                     /locus_tag="JN749_000158"
     CDS             complement(47350..47871)
                     /gene="aroK"
                     /locus_tag="JN749_000158"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HAY7366701.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48272..49510)
                     /gene="hofQ"
                     /locus_tag="JN749_000159"
     CDS             complement(48272..49510)
                     /gene="hofQ"
                     /locus_tag="JN749_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HAY7366702.1"
                     /translation="MKQWIAALLLMLISGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49422..49826)
                     /gene="hofP"
                     /locus_tag="JN749_000160"
     CDS             complement(49422..49826)
                     /gene="hofP"
                     /locus_tag="JN749_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HAY7366703.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADIRRTGGKAAKSDADGG"
     gene            complement(49816..50256)
                     /gene="hofO"
                     /locus_tag="JN749_000161"
     CDS             complement(49816..50256)
                     /gene="hofO"
                     /locus_tag="JN749_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HAY7366704.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50240..50779)
                     /locus_tag="JN749_000162"
     CDS             complement(50240..50779)
                     /locus_tag="JN749_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HAY7366705.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50779..51558)
                     /gene="hofM"
                     /locus_tag="JN749_000163"
     CDS             complement(50779..51558)
                     /gene="hofM"
                     /locus_tag="JN749_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HAY7366706.1"
                     /translation="MTFKIWQIGLHLQQQEVAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51678..54230
                     /gene="mrcA"
                     /locus_tag="JN749_000164"
     CDS             51678..54230
                     /gene="mrcA"
                     /locus_tag="JN749_000164"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HAY7366707.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54395..54955)
                     /gene="nudE"
                     /locus_tag="JN749_000165"
     CDS             complement(54395..54955)
                     /gene="nudE"
                     /locus_tag="JN749_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HAY7366708.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55275..57410
                     /gene="igaA"
                     /locus_tag="JN749_000166"
     CDS             55275..57410
                     /gene="igaA"
                     /locus_tag="JN749_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HAY7366709.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57475..58143
                     /gene="yrfG"
                     /locus_tag="JN749_000167"
     CDS             57475..58143
                     /gene="yrfG"
                     /locus_tag="JN749_000167"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HAY7366710.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58154..58555
                     /gene="hslR"
                     /locus_tag="JN749_000168"
     CDS             58154..58555
                     /gene="hslR"
                     /locus_tag="JN749_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HAY7366711.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58580..59458
                     /gene="hslO"
                     /locus_tag="JN749_000169"
     CDS             58580..59458
                     /gene="hslO"
                     /locus_tag="JN749_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAY7366712.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59593..61317)
                     /locus_tag="JN749_000170"
     CDS             complement(59593..61317)
                     /locus_tag="JN749_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HAY7366713.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61696..63318
                     /gene="pckA"
                     /locus_tag="JN749_000171"
     CDS             61696..63318
                     /gene="pckA"
                     /locus_tag="JN749_000171"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAY7366714.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63394..64746)
                     /gene="envZ"
                     /locus_tag="JN749_000172"
     CDS             complement(63394..64746)
                     /gene="envZ"
                     /locus_tag="JN749_000172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HAY7366715.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64743..65462)
                     /gene="ompR"
                     /locus_tag="JN749_000173"
     CDS             complement(64743..65462)
                     /gene="ompR"
                     /locus_tag="JN749_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HAY7366716.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65654..66166
                     /gene="greB"
                     /locus_tag="JN749_000174"
     CDS             65654..66166
                     /gene="greB"
                     /locus_tag="JN749_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HAY7366717.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANW
                     YVNAIEYVKP"
     gene            66263..68584
                     /locus_tag="JN749_000175"
     CDS             66263..68584
                     /locus_tag="JN749_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAY7366718.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69022..69249
                     /gene="feoA"
                     /locus_tag="JN749_000176"
     CDS             69022..69249
                     /gene="feoA"
                     /locus_tag="JN749_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HAY7366719.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69266..71587
                     /gene="feoB"
                     /locus_tag="JN749_000177"
     CDS             69266..71587
                     /gene="feoB"
                     /locus_tag="JN749_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HAY7366720.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGMGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71587..71823
                     /gene="feoC"
                     /locus_tag="JN749_000178"
     CDS             71587..71823
                     /gene="feoC"
                     /locus_tag="JN749_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HAY7366721.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72026..72904
                     /gene="rpnA"
                     /locus_tag="JN749_000179"
     CDS             72026..72904
                     /gene="rpnA"
                     /locus_tag="JN749_000179"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HAY7366722.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            72977..73246
                     /locus_tag="JN749_000180"
     CDS             72977..73246
                     /locus_tag="JN749_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HAY7366723.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            73240..73605
                     /locus_tag="JN749_000181"
     CDS             73240..73605
                     /locus_tag="JN749_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAY7366724.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(73606..74394)
                     /gene="bioH"
                     /locus_tag="JN749_000182"
     CDS             complement(73606..74394)
                     /gene="bioH"
                     /locus_tag="JN749_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HAY7366725.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            74432..75115
                     /gene="gntX"
                     /locus_tag="JN749_000183"
     CDS             74432..75115
                     /gene="gntX"
                     /locus_tag="JN749_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HAY7366726.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75174..75749
                     /gene="nfuA"
                     /locus_tag="JN749_000184"
     CDS             75174..75749
                     /gene="nfuA"
                     /locus_tag="JN749_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HAY7366727.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76110..77414
                     /gene="gntT"
                     /locus_tag="JN749_000185"
     CDS             76110..77414
                     /gene="gntT"
                     /locus_tag="JN749_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7366728.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77459..79543)
                     /gene="malQ"
                     /locus_tag="JN749_000186"
     CDS             complement(77459..79543)
                     /gene="malQ"
                     /locus_tag="JN749_000186"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HAY7366729.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTTTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79553..81946)
                     /gene="malP"
                     /locus_tag="JN749_000187"
     CDS             complement(79553..81946)
                     /gene="malP"
                     /locus_tag="JN749_000187"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HAY7366730.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        19755 a        22362 c        20770 g        19459 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaacagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctgctcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcactcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggatcac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccacgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccaac cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctttg cgctttgccg tctcgcccag taaataatga tggaacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgtt
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccccgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgcca gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat agcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc agatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtacgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgat cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctggaa cgtatggagc tgattgcccc
    11041 cgcacacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaagg tcagcgcagg ctatggcgat cgcattattc tcgactcgat
    11161 taaattgaat ctggtccccg gctcgcgcat tggtctgtta ggccgcaacg gcgcaggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcaaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttcctggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaggccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cagtcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccaaa accggaaagt
    12961 ctcccctcgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggaact gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aatagtgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtagt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcggtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccttg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtacctgg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgctg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tttgctatta tgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aagtcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacactt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccaccgctg ctggtgcgcc agcaaaaagc ggtcgattta attactcagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaacaatac cgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttgcagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaaactggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccgttgc ttccggcact
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgt gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtagcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg caacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actgttgtgg ctgaacggtg agcagtacat ccttccacgt cttatcgata ccattattgg
    17161 ttgtttgatt gctttcggtg gtaccgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgatg ctttagaagc ctatcaggaa gcgattcgct tgattctgag
    17281 cgaggacccg caaccaacgc cactggcctg gcagagaatg cgggtaaatc aggcgcataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcattcaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggccatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatacaggc taaaccatcg gataaggcat tcacgccgca tccgacatat
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccctc atcgatatcc gcatcttcca ccaccagcga cggtgctaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccaa cctctgcacc cgcatacagg aaatcacgcg
    18001 cccgaccttt gtagtgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaagtcag aatatccggc gtcacaccgt agtacctgta agcaaacaaa tcgccagtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatac ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgca tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt gcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tatgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 ggaaatcaga gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcactatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtc gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tggtacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac atcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtaatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc agctgtgagt tcccggcact gggcaaacgt
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt gctgaacgct
    24061 atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg tagcggtaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtaaa
    24661 tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 ggcgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta ttttttgttt
    25861 ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg cggctaatgc
    25921 tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca gctccgccat
    25981 ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta atttgctcga
    26041 cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa cgctggtgat
    26101 tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg gggtaaaagc
    26161 gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc tgggtaacct
    26221 ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc tgctgccggt
    26281 agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg caatggtact
    26341 gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga tggcgctgcg
    26401 cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat ttatcgcgtc
    26461 cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt tcggcaacca
    26521 cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga cgctgggtaa
    26581 tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc cgaaagcgaa
    26641 tcgtccggtt gcggacaaat ttaatcaaac tgaaacggct gccggttaat tactaagggg
    26701 tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact gtctgattgt
    26761 cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag gcgctcgctt
    26821 aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg caggcatgtt
    26881 aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc tggcgattgc
    26941 agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag ctcgtcgcat
    27001 cttctgtaac gtagtcgatg cgccgaaagc cgccagcttt attatgccgt cgattattga
    27061 ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc tggcgcgcct
    27121 gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg cgaaatatgc
    27181 cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc gtcgccgttt
    27241 ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca acgatcagaa
    27301 agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc gcggtgaagt
    27361 ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag gactgcaaca
    27421 aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata ttatgaatct
    27481 ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc actgtgtacc
    27541 gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac gcgtggtgcg
    27601 gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg aaacactgtg
    27661 caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg gttgctctgc
    27721 ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct taattaccgg
    27781 acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa aacagacgct
    27841 ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga ttgaacacgg
    27901 tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca cgcagcgcgt
    27961 gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca gtccatcgct
    28021 aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct ccaatcatta
    28081 atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat attcagcaaa
    28141 taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc gttacagcga
    28201 cttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta tcgttcttaa
    28261 cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata atacataggg
    28321 aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg ctacgttaac
    28381 tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact acctgctgca
    28441 cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac agtaagtctc
    28501 tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt tccgagggcg
    28561 tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt taaattcaat
    28621 tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg atcttcctcc
    28681 acattacaca acatcatatg ttgttatatt catcatgcat tgtcatgttg ccttttaaat
    28741 gactgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta tgggaagcca
    28801 ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg ggacaaccgt
    28861 cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca cgccgtggct
    28921 tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt gcgtctatgc
    28981 ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga aaaatgccgg
    29041 aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg atgcgccgtc
    29101 attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc ctatcgatcc
    29161 gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc tacacctgcg
    29221 ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa ttatcccgtt
    29281 cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg ccgcccttac
    29341 caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga tctctgccac
    29401 cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa ttaaaaaccc
    29461 cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg tgctctacac
    29521 cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca attctgaaac
    29581 gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg ggatctttgt
    29641 tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga tgtaccagcc
    29701 gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg gccatgtgca
    29761 tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg ggatcttctt
    29821 catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga tgtgtttcaa
    29881 aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt acaaaccgct
    29941 gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa gcctcattct
    30001 ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg tcatcgttat
    30061 tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc tcaacgcttt
    30121 gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac cgtggacttt
    30181 ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga cgttccgctg
    30241 gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta tttcgttgcc
    30301 tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg cttttccgat
    30361 cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta tcgcctcgac
    30421 gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt aatcaaagcg
    30481 ctggagctgg gccgggcctg cggcgcactc actgcggcgt tcaccaaacg cgcggacagc
    30541 ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat ctgggaaatt
    30601 cacctgctgc tctgctacag cgtggtgctg gagatgatca ccctcctcgc gccgaacgcg
    30661 gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg tcatctggta
    30721 cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg gccgatgatt
    30781 tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat tgtaacgctg
    30841 atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg ccatggcccg
    30901 ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga tgaaagtcgc
    30961 cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt gatcgtcatc
    31021 gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct gatgttcgtg
    31081 ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc ggatgaacgc
    31141 cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa cgggccggat
    31201 ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc agcggctgcc
    31261 tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg aaatttgggg
    31321 cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac aaatcaaggc
    31381 gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca acggctatcc
    31441 gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca tgatcaagct
    31501 ggcgatatat atggcaaaag agatgaacgc gggttatacg ctgatttctg cggcccacgc
    31561 gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact taagcgaact
    31621 gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa cgccgtatga
    31681 atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg tgccttcgcc
    31741 gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac cggtgatgag
    31801 ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca gcgacggggc
    31861 cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac tgatgcgcga
    31921 tattattgat cggggctatg agggttactg tacggtggag ctggtgacga tgtatatgaa
    31981 cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc tgccggagga
    32041 tgagagatga aaaccctagc gacaatcggc gataactgcg tcgatatcta cccgcaactg
    32101 aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac tcgctacggc
    32161 atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa gctgaagcag
    32221 gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg cgttaccgca
    32281 caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac cgaaggcgtg
    32341 atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta tgacattgtg
    32401 cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc tgcgggcaaa
    32461 cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac actggtgccg
    32521 catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg tctgaagatg
    32581 aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga aaacggcagc
    32641 attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac ggttatcgac
    32701 accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc tgcggggatg
    32761 acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat tcagtaccac
    32821 ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct gttacttgcc
    32881 ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat tatgtcagct
    32941 acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct gctggatgat
    33001 atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga gctttgtaca
    33061 caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt ggcagacggc
    33121 gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt tgaaaacgcc
    33181 ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaaggc gacgaaagag
    33241 aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct gaacatcccc
    33301 ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga gccgctgttt
    33361 attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat ttacgtcgaa
    33421 ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt cagcgacaaa
    33481 aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa atgcgaactg
    33541 ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa accggtgcac
    33601 gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa taaaagacat
    33661 taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc gcactgaatt
    33721 ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag gggagcgttc
    33781 agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac tcgcaatact
    33841 ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa tgcgaatcgc
    33901 acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat agaagagcgg
    33961 cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac gcttttgcgc
    34021 ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca ccttcgactg
    34081 cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa gcaaagccag
    34141 caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca ccagaccacg
    34201 caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa gcgtggcgcg
    34261 aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg gcccaaacag
    34321 cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc gcgctacctt
    34381 catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca acgttgccag
    34441 cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca ccagcgcggc
    34501 atcaggttgt tgctcgtcga cgacctgctt cagtgccaac aggtcattga aatcagcagt
    34561 aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa caggcgcgtc
    34621 atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc gaatcgcgcc
    34681 agtccccgcg ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag ccagcacctg
    34741 ctccacccgt tgggtaatac gcggttgatt cagacctgga gttaacccta aatcaccgcc
    34801 agcaagaaac tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg caaactgctt
    34861 ttgctgcgct tcagcaagag tcaggctttg caaaggaaac gtcttcatag gagccttcct
    34921 taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc ggtgatcatt
    34981 actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc gttgaggaag
    35041 taaatagcaa ccgcgataga gaatccggta taacccgcca tcttaattgc tgcgaaaatc
    35101 gaaccgacca gcagcgccat ttccatcagc atattcatgg cgttacggat gttatccgac
    35161 gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag cagcaagact
    35221 tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg cgagagatag
    35281 cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc taaggcggtg
    35341 gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc cgcctgatta
    35401 atcagcgttt gcgattgttc cggcgtcacg cctgcggaat acgctttctc cagtgtgaag
    35461 atcgaaactt cgctaccagc aaaaatcttc atactggcaa cggcggcaat caatgctccc
    35521 acgatggcga tataaggtaa gtttttgata atccgcgacg tgcgctcttc aaacaccgaa
    35581 agcccgctgg catcgatgtc attttcatca cgatgacgca ggtcgtgagt tatcgcgatc
    35641 cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag atgtgggaaa
    35701 tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag actttgcttc
    35761 cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga aaccaccggc
    35821 gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag cagctggtta
    35881 actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc aaacgccatc
    35941 aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa ccatgcgttg
    36001 agcagccagg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc aatgctcaga
    36061 ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag ttgcggcagg
    36121 atcgggtgga tgccgtcatg aaaaacagcc gccgagcgat gcgccagaag cgatgtcata
    36181 cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt gccttttatt
    36241 tcagatggtt aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa aggccaaacg
    36301 ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta tgccgcgccc
    36361 gcaccggtat tacacgcgcc aatgtaataa tctagttgcc cggatttcac cttcattgcc
    36421 gcttccatgt cgttgtgaat gaaaacttca aaacagccag gagccgtcgc ttcaatggtt
    36481 tttttaatct gctcacgctg taagcctgca acgccaatct ttttcatgag tctgtcctta
    36541 ttggaaaaat tgagagggat tttcacgtaa catcacatcc acatcggcct gactgaatcc
    36601 tgactggcac aattgcggaa taaaggtggt caataaatag tcatagccat aaccaccatt
    36661 ggcttttaaa tgggagcggc gcgtaatatc catcgacagc atgacgcggt tcagcaaccc
    36721 acggtcacgt agcgcgtgga gcatcgcaat gcgcttttcg tccgggtagt aactgttctt
    36781 accaatggtg tcgaactgca cgtacgcgcc gagatcgatc atcttcaaaa tgttgtcgag
    36841 gttgtctttc agatcgcagt gaccaacggt gacgcgcgaa agatcaactc cgtgggcttg
    36901 caacagagcc agttgttcca gccccatggt gctgaacgac gtatgcgtgg agatcgggcg
    36961 tccggtctgg ttatgcgcca gcgcagcggc aataaatacc ttctcttcca gcggcgtaat
    37021 ctttccttcg ctggagccga tctccgcgat gatcccggct ttcagctccg tgccatcgat
    37081 accctgttca atttcatcga ccatctcctg cgccagttcc tgcacgctgc gggtcaccac
    37141 atgttccggg aaaaacgcat cctggtaata accagtacag gccaccacgt tgatcccggt
    37201 ttcgcgcatt acatcgagca taaattgcgc attgcgcccc atgtaacggt tggtcatctc
    37261 aatcacatta cgtacacccc gggtcatcag gtcgttcatc tcctggcaaa tgaacgcata
    37321 ctgatcaagg cggcagtcca cgttgttttt aaagccggag agatcaatat gcagatgctc
    37381 atgggccagg gtgtaacccg tcggatcaaa actcataggg tgtctcctgc aaaccggagg
    37441 gaggaaagaa aagagcgacc attttgtggc ggtggcgcac ggaaaaattc acacacggta
    37501 gctcccacat cagaaagcgt ggtgcgcaca ccgagctgcg tggcgaccag cccttgctga
    37561 taaaccagca ctggcaccac ttcacgggta tggtggctgt gaccaatggt cggatcgtta
    37621 ccgtgatccg ccatcacgac caggcaatca tctggctgca tcgcctcaac aagccgggca
    37681 aggttacggt caacgacctg caaacgttcg gcataacgtg cgacgtcttc tgcatgacca
    37741 gcgaggtcgg tttcctggat attgatgcaa ataaacgccg tcggatgggt gttaaattcg
    37801 tcgagggtga tatccataat ccgctggcta tccaccaggt tttgccagct cacaccataa
    37861 ggattgctga cgatatctgc caccttaccc accagcacgg ttggcacgcc cgcttcatac
    37921 agtttttgcg gcacctgcac tttttcatca acaccgtagc ccatatgcac gacctggaag
    37981 ccgttgtcat aagcgccaga acgcggcgca ttaataccaa taaagcgccc ttctttgctt
    38041 tctgcggcat cgagaatacg ctgactgttg gttaacaggc cgccaaatgt aatgacccga
    38101 ccgacctgta cctggtcacg cacgatacga ccaattttga ttgcgtcgtc aaaagagatc
    38161 acagagagat tggcggtaat gttatagact tggcctaaat ccgcctcgag attatcgcca
    38221 atcgcaaccg cctgattgac ccacagaaat tgcagatcat cgccacggcg ctccacctgc
    38281 cagccagcgg aaactaatgc ctgctcaaca cggtcaatca catcgcgaaa aggcatccgc
    38341 agcggcggta acgggcgcgt gcctaaaatt tcctgatgcc ccataaaggt atcgccacct
    38401 tcatgttgca gctctgccac gccccaggtt gcggaatctg acggctgcat atcgcctggc
    38461 gcataaccca atgtgttgat tagccccagc ttctccaacg ttggtagctg caaatgcggc
    38521 aactggctca ggatgtgacc acaggtattc gccccggcat cttgcggacg caccagcgtg
    38581 acatctttca ttgcccctac gccaaagcta tcaatcacta acaccacaaa tcgcgccatt
    38641 tatgccttca gagggttgcc gaggctgtca taacgaccaa ctatttctgg ttcgccgcgc
    38701 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcgaaaacag
    38761 agcaccactg cactgctcac cggatactcg cctgccagcg gcagggtata gtcgatactg
    38821 ctgtcatcca cggttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38881 agcgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    38941 cggaaatgat gggagatttc acttaaccag agcatcgcga tacgttcagg ctgatcgccc
    39001 tgctggtttg ccggaatagt accggtcagc gcatgaccgg gttcggcatg agtcgcaccg
    39061 tattccgcca gcaatggcag cgaagtgcag ctggtcgctg aaggtacgtt cagttgctca
    39121 atcgcaatac cagatttcgc cagttgatcc cgtgcttgta ccagcgtgtg aagattcggt
    39181 gttggcaaaa ctttcccggc agcctcatcc caaagcaggc aagggaaatg ggttagtccg
    39241 gctaaatgca gccccggcag tttcttaatt tcagcaatca cctcgtgcag cgaatgctgt
    39301 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39361 acaggctgga cccgtccggc cttaaccgct gccgcagaaa cttcccgtgc tttgtcgaga
    39421 gtaaacacgg tgatgatgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39481 atttgtacca gatgcccctg atgcgccact ggcaaaccag cgcggcgcat gactcgcgcc
    39541 tctttgtaat ccaccgccac aatgccgctg tagcctaatg ccagcaattt ttccgccagc
    39601 cacggattac gaccaaattg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39661 gtctcaatga gccgcttacc gttttccagt atctgatcca cgtcgatcac ccagctgtcc
    39721 ggggcgattt tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39781 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39841 atttttttaa atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39901 tagcagccat ccataatcca taaagattgg ccagaaaata gccttcttca cacgggttaa
    39961 cttccagagc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40021 tggattgcgc cagttccgcc agcaactcgt tatccagcgg ctctatttct tcaccacggc
    40081 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40141 ccggcagatg gcactctttt tccagtacgt tgacgacctg catcatgcct ttgcagacgt
    40201 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40261 ttcgtttaat aactgggttc ctgttcccca ctcaccaccc gttgttgatg ggcaagcagg
    40321 gcgtgtttat ctacaacggc acgcagcaat tgttgcattg ggtaatcatc gactcgcgtc
    40381 aggaccacgg cttcggtggc cagtaaaaat cgcggatcgt ctgttagcgg cgtcgcctct
    40441 aatcccagca tggtcagttc gttttccgcc accacgttcc agatcaccgc atcgacatcg
    40501 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgactcgttc cacatcacta
    40561 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacgactatc gagacctact
    40621 cgcttaacat ttccagactc acctttgcgg catatcagtt gatgttcacc aacatacgta
    40681 tgcgggccaa gctcgagggc gatgcacaaa ttgttttggc tgagataact ttccgccgcc
    40741 aggcgggaaa ctactgccat gtcatagaca ccgttaagta aacactccac gcgaatatcc
    40801 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgggcttt caatccgctc
    40861 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40921 tccacatgac tcagcaatgc tttgttatcc atctcgacaa gataactgcc attgcgccca
    40981 cggcgttcaa tccgtatcgc tccgcttgat tccagcgttt tcaacgcggc ctgcgtcagg
    41041 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41101 ttttcaccca acaaataacg ggccagtgtc gtgatgacga cgccttcttt tttgataaac
    41161 gttcgacgca taatatattt tcaataaaat gaatatttat atcttcatta aaatgaagat
    41221 aagtggcgac gacgagatgt ggaaacggcg aggcgcttca cattttttcg ggttgttgcg
    41281 aaaagaggat aaaaaaaccg ggtttccccg gtttcagagt gatgacgaag tgatttattc
    41341 gacaatgcga gcaaagggta attcccactg gcgcttgcct gctcccctca ccctaaccct
    41401 ctccccaaag gggcgagggg accgtccgag cgttttgata ttacggcttc gccacaaaac
    41461 caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct ttttctgcgc
    41521 cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga tagcgttcct
    41581 gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga tgaccataca
    41641 tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc gcagaaagga
    41701 tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact accggcggct
    41761 cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga tcttccagca
    41821 ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc ggctccagca
    41881 gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta aagatctcgc
    41941 catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc tgtttctggt
    42001 cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc atcagcaccg
    42061 gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta tctttaaact
    42121 gcgtcatgcg actcagttcg ccgaagtagg tatagcagtt cagtgcccag cctaactgcg
    42181 catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag
    42241 ccagatacaa ggccagcgta tccaacgtcg ctttacgcag cttctgtgca tcctggcgca
    42301 cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc tgcatgttta
    42361 cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag ggctgtgcgc
    42421 cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg cggaagccct
    42481 aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct gagatcgata
    42541 gcctcgccgt agttatatcc gtaggttaag ccaactgatg ggcaacctgc cgcttttgcc
    42601 gcctgaatat cattgcgtga gtcgccgaca aacagcatct gttgtggggc aattcccatc
    42661 cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac atcatcgccg
    42721 ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagcgg cgcgacgaac
    42781 ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa cgcgcccaac
    42841 gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc atagtagcga
    42901 tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc
    42961 ggtttcccca ttgttttacg cagagtcgca cgttcctgac gcgcccaggt caatgcgcgc
    43021 tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc acctgcgacg
    43081 ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccagg agcactgtcg
    43141 accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt attcatgact
    43201 tacctttgcc agttcactgc gcatttcatc aatgactttt ttatagtctg gctggtcgaa
    43261 gattgccgaa ccggcgacga acatatccgc gcccgccgca gcgatttcgc caatgttgtt
    43321 caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt cgatacggcg
    43381 acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg
    43441 gttgacggac atcagcagga tcacatccag cttatccatc acgtaatcca gatagctcag
    43501 aggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa tcagttgcag
    43561 cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg caccagcggc
    43621 agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt cgataggggc
    43681 ggtaatgccg tagttacgca aggatttcag caccattggc ccaatcgtca gattgggaac
    43741 atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca gggcttttgc
    43801 ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa tcaaatactg
    43861 tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc tggtttgtac
    43921 aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat actgcgtcga
    43981 actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat cgtatcgtga
    44041 ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc cgccagatgc
    44101 gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc ggtcgcagac
    44161 agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg ctccatgcta
    44221 tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac
    44281 tctgcttccg ggaaataggg ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc
    44341 agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa cagtaccgcc
    44401 cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta aacctcggcg
    44461 caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc agtacgcatc
    44521 ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat ataacgagaa
    44581 aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag acattcgccc
    44641 ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc ccacttcaaa
    44701 aaagcgcgat ttttcttcat gccgactaac taattacacc ttctccggct gtggagaaag
    44761 ctccgacagc atccagcgct tcaggcagct cccgcaacga taaaccattg cgggagataa
    44821 ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg ggtttttggc
    44881 ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg aagcgtacac
    44941 gccagaaacc aggacatacc acggctgacc attacgcgtc gtttcataga caacgtagtt
    45001 tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg aactgctcag
    45061 ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac ctgcggtctt
    45121 ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta ctggagccgt
    45181 agtcgccgtc tcttttggtg ctggcgtaga agtcgcagcc ggtgccttcg tgctcgccac
    45241 aggggcagct ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct ccgttttcac
    45301 ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgaactgggc gcgtagtgga
    45361 cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat tgcgaacagg
    45421 cgcaaccgtt gcgggttcag tcggcaacgt ggaattgacc gccacattgt tcagctgttg
    45481 ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa cacgttgttg
    45541 accatccgtt gccaccgggg tttgcccttg agtcggcgta gaagagatcg gtggcagaga
    45601 aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca cgccattcgc
    45661 ctgatcggtc gcattgccag caagatcaat actcttctcg ccagacgcgg tttgatcgct
    45721 ggaagtggtc gagggggctt ttagcgcaga accgataccg atgatcaaca gcagtagaac
    45781 cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg ctgctttttt
    45841 gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt cggattcgtc
    45901 aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag tcggacggcg
    45961 atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc cacgttcagt
    46021 acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc tgggatcggg
    46081 tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa ggttaatgct
    46141 taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct gacctttctt
    46201 gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc
    46261 tgcgaacttc actcttacca attgccaacg gaagaattaa gcgcatctct cccgcaagga
    46321 ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat
    46381 tgaccggtaa cccagcccgc gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa
    46441 actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg accgcttcac
    46501 catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag gtgtgtccca
    46561 gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc agcgacaact tctgccttca
    46621 gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc aacaacgcat
    46681 ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg tatttgatga
    46741 cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag tcgagatcca
    46801 ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg ggatggttga
    46861 ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc gggacttgaa
    46921 tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg cccactacgc
    46981 cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac aacgccgtaa
    47041 agacggtatc gagtacagct aggcttttat actgctcgcc gtcagggagg ataacgctat
    47101 cgacgttaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac agaggagcca
    47161 gggtttcgct ggtgaccaac atgacctgct cgcccgattt cagcggtaag aatgaagctg
    47221 gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg agagtaacga
    47281 caatcctctc cataacgcga catccacctt aattactgta cccgcagacg agtgtatata
    47341 aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca ctttagcgct
    47401 ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat tgcgttcatt
    47461 ggccaacgct tccagaactt cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt
    47521 atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc
    47581 acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccgccgc cagtagccag
    47641 cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc
    47701 ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt tctcaatctc
    47761 ttgatcggaa tcgtaaaatt ccatattgag tttttgagct aactggcgcc caatagtgct
    47821 ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca ttttttcggt
    47881 actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc gcaggacatg
    47941 aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa tactccagcg
    48001 ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc aagcgcactg
    48061 ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa aaagacagca
    48121 aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga gcaccaactc
    48181 tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc
    48241 agcataaaaa cattaccgct tttacggctg tttactcact ggaaaccagt cgtggcgtga
    48301 taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat aattgcccga
    48361 accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt ttacgggtaa
    48421 aaatgccgcc cagcgccaac gtttctccgc tttttacctc gacctgcgtt tcgatctcct
    48481 gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc ggaacgttct
    48541 ggctgatgtg taatttcagc cggatgcgac ctttttgtaa caccgtgggc gtgacctcca
    48601 tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg ctggaaacct
    48661 gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag gccagcagac
    48721 gcggactggc gataatatcc agctgctgtt tttgttcgag cgcggaaagc tcaagatcca
    48781 gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc gtcgctacgg
    48841 agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc gcatcggcca
    48901 gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca atatgcgccg
    48961 acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca agcgcgctta
    49021 acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg gtcatactcc
    49081 ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg gcgtattgca
    49141 gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc tccgcctcct
    49201 ggcgggcgat attgtcattc tgccaggcaa tggaatgcac tgagagaatg ttgccttcct
    49261 gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc cagggaacat
    49321 ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg ttcaacttct
    49381 cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc atcagcgtca
    49441 ctttttgcgg ctttgccgcc tgtacgccgg atatcagcat caacagtagt gcggctatcc
    49501 attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc gcagtttgtc
    49561 ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc gttttccagc
    49621 acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac accgatgatg
    49681 cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct aatccggcat
    49741 agatcttccg gcgatttaaa agggtcacgc ataccggtta aaaggcacaa tgcaatacct
    49801 gccaacaacc agcgtttaac cctcatgcgg catctccagt tgtagcgtga acaaaagatc
    49861 atcaccttcc acgcttaacg aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt
    49921 aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc cctgcgcgga
    49981 tggatgccag gaaaccagtt gcgcgccgga taactgaaaa tccagtggcg aaaagggcag
    50041 cgttttttcc tcgctgaagg gagtggtgtc taccaggcga tacagtgcgg cccactgctg
    50101 gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg tcgatgatag
    50161 aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc ggaggcgggg
    50221 tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt gttaactgat
    50281 actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga ttgagatgaa
    50341 aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg cttgttgtca
    50401 gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag gcgtgctcgg
    50461 gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat tgtcgctggc
    50521 gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc cagcaaacgt ggcttcgtta
    50581 tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aactgcgtct atgcgcgctt
    50641 cggcgctgcc tgtcagacgc agtattagcg ttatcccgac ggccagcagc agaggcgcaa
    50701 cgaacatcag caaccagaaa cgcagaaaag cggtccggcg ttgctgtcgc cagggcaaaa
    50761 aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg cgatggcaaa
    50821 gtctccacca ggcggcggta gcggcggctg acgaacagaa acggcctccc aggggtcaaa
    50881 tccgccttcg ccacatatcg cgacgctgtc gggatcaacg gataacgctg ccgccagctc
    50941 cttcgcgcta gtcatcccca ccgccagttt gcgcccccag ctatagcgcg tcgcccacag
    51001 ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa
    51061 tcgctgtaat gcactggcat ccggggtgat cgcactaaca tgaacacgca acctttctgc
    51121 cagcgtaagc agcgttgcca gctctttgct ttgcgcggca gtcacgttat aagcggggct
    51181 gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga tccatatcca gctcgcgggc
    51241 catcgtccct gacagccagg ccgtttgctc ccgctcacca agggacatcg acgggcgcgg
    51301 aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat gacgctgcgg
    51361 cagttcgcga ctccacggta acaacgtttt agccagctgc tgcgcatcaa caatccgccc
    51421 atctttgata atgtcgtgcg ccagcggcaa ccgccaccag cgttgcaaaa agcattcttt
    51481 tgcgccccgc acgatcgcaa ccgctgccac ttcttgctgt tgtaaatgca aaccaatttg
    51541 ccagatctta aatgtcattg tgatgatctc cttatcaccc gtcactctga cgggtatatc
    51601 aatgcgtctg gcttgccttt atactaccgc gcgtttgttt ataaactgcc caaatgaaac
    51661 taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca gtctgttgca
    51721 ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca caactgccgg
    51781 atgtggtgac attaaaagat gttcgcctgc aaattccgat gcagatttac agcgccgatg
    51841 gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc
    51901 caccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac gagcatcacg
    51961 gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc ggccacgcgt
    52021 cacaaggggc gagtaccatt acccagcagc tggcgagaaa tttcttcctc agtccagaac
    52081 gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa cagttgctga
    52141 cgaaagacga aatcctcgag ctttatctga acaagattta ccttggttac cgcgcctatg
    52201 gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg acgctgaacg
    52261 aaatggcggt gatagccggg ttgccgaaag cgccttccac ctttaatccg ctctactcaa
    52321 tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat gaagggtata
    52381 tcacccagca acagttcgat cagacacgca ctgaggcgat taacgctaat tatcacgcgc
    52441 cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag atgtataacc
    52501 gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc acccgcaaag
    52561 tgcagcaggc cgcgcagcag gcggtacgta ataacgtgct ggactacgac atgcgccacg
    52621 gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg gataacaaca
    52681 agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct gccgcagtca
    52741 ccagcgccaa tcctcaggga gcgacggcga tgctggcgga cgggtcgacc gtcgcattga
    52801 gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag ggaccgacgc
    52861 cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt caggttggcg
    52921 atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg atcaatccgc
    52981 aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc aagtttaacc
    53041 gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc tacaccgcgg
    53101 cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt tctcgctggg
    53161 atgcaggtgc cggttctgac tggcagccga agaactcacc accgcagtat gctggtccaa
    53221 ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt gcaatgcggg
    53281 cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg gcgcaaaaca
    53341 ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg caggtggcgc
    53401 gcggctacgc ggtcatggcg aacggcggct tcctagtgga cccgtggttt atcagcaaaa
    53461 ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat
    53521 gcgatattcc agtgatttac ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg
    53581 ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg
    53641 agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca
    53701 tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc tttggtgaac
    53761 caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc gatatcggcg
    53821 ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg
    53881 ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt catacaacgg
    53941 cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag agtgcccagc
    54001 ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc
    54061 cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta gctaatggtg
    54121 gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag gcggtgcacg
    54181 aggtgggaac gaccattatc gataatggtg aggcacagga attgttctga ttaaaaaggc
    54241 gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga tgcgggtgaa
    54301 cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc ccataagcct
    54361 gataagcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc cctttcaacc
    54421 attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca gggtcttcca
    54481 gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct ggctcatcgc
    54541 cttccagcga ttccgggtag agatcttgcg cgaccacgat attcatttta ctggaaaagt
    54601 aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca aatccaacct
    54661 cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca attaatcctt
    54721 tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc aggatcaggt
    54781 gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc attcgttcat
    54841 aaacacgccg cacgccattg ctgaactcca gatccacact ctcgacggta aacagtcggg
    54901 aacgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg ttcatcgtgg
    54961 gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc ggcaatgtga
    55021 attgcatgtt atttacattt atgtaactta ataaataatc gtcctcaaat caaattaaaa
    55081 gtcaataggt tgaaataact ccaggaattt gctgatattc cgccttcaga gggtttgcta
    55141 tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac gcttgccgat
    55201 agccaaccgc agaatcatgt attgtgtccg gtgcgactga ccacgcctga cagactaagt
    55261 aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg gcctgctcac
    55321 tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc tggaccaacg
    55381 ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc gttgaaaatt
    55441 atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct agcgcggcac
    55501 cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca cacgctatca
    55561 cacgttacgg catctctacc gacgatccga ataaatggcg ttactacctc gattcggtag
    55621 aagtccacct gccccccttc tgggaacagt acatcaacga tgagaatacc gttgaactga
    55681 ttcataccga ttcgctgccg ttggttattt cactcaacgg tcatacgctg caggagtaca
    55741 tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg tcgattcgcg
    55801 gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat gaagaatatg
    55861 cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc ttcctgatgt
    55921 tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc ggcgcattac
    55981 tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc tccctgcgag
    56041 aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa aacgatcagg
    56101 aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca cactggcagc
    56161 catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatcta gaccgccacg
    56221 tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt ccgttacagc
    56281 actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt atgctgttat
    56341 tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa ggcgcgcaga
    56401 ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta ggcgatacat
    56461 tgcgtattag cggtacggga atgtgtaata ttcgcacttc cggaacctgg agcgcgaaaa
    56521 ccaattcacc ttttttacca tttgactgct cgcagatcat ctggaatgac gcccgctcat
    56581 taccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa gcagttaatc
    56641 gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag tgcctcatta cgttcagcga
    56701 ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg aagacagcgg
    56761 atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc aatctcggca
    56821 acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc tgggaagctc gatggcgtga
    56881 atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca acctccaccg
    56941 cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt ccggcccccg
    57001 gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc tggccttcgg
    57061 catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa ctggcacaaa
    57121 tgctgatgca tacgccgttt aacgccgaag gtatcgtcac aaaaatcttc actgacgcca
    57181 atggtacgca gcatattggc cttcatccga tcccggatcg ttccggcctg tggcgctatc
    57241 tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat ggcgtacagg
    57301 cctggcgtcg ttaccagcgt catcgcactc gcatgatgga gattcaggcc tattatgaaa
    57361 gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa cacgtttgcg
    57421 cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc cctcatgcat
    57481 atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg cacgttgctc
    57541 gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg gggcgcgaaa
    57601 aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca cgacgtacag
    57661 catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct ggatatctgt
    57721 gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg aagataccat tccgtttctt
    57781 gaggcactga aagccagcgg taagcagcga attttgctca ccaatgctca tccgcacaac
    57841 ctggcggtaa aacttgagca tacgggtctg gacgcacacc ttgatttatt actttccacc
    57901 cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc cgaagctacg
    57961 ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct cgatgctgcc
    58021 gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg gattgccgag
    58081 aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc ctcgctaatg
    58141 tgaaggagac gccatgaaag agaaacctgc tgttgaggtt cgactggata aatggctatg
    58201 ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattaaag gcggtaaggt
    58261 gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg ccacgctcac
    58321 tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg aacagcgtcg
    58381 ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag agaaacgcga
    58441 aaaaatggcg atggcacgta aacttaatgc cttaaccatg ccgcacccgg accgacgccc
    58501 ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg aataactgtc
    58561 acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc tgtttgaaaa
    58621 ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga tccttgagaa
    58681 ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg cgaccagcct
    58741 gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcagg gcgacggtcc
    58801 gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg tggcgcgcgt
    58861 gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg gttacgtggt
    58921 gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtagttggtc tggaaggtga
    58981 taccctggcg gcctgcctag aagattactt tatgcgttct gaacagctgc cgacgcgcct
    59041 gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt tgttgcaggt
    59101 aatgcctgcg caaaataccc agcaggacga ctttgaccac ctggcgacgc taaccgaaac
    59161 catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc gtttgtatca
    59221 cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca cctgctcgcg
    59281 tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa gaagttgata gcatcctggc
    59341 ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc tgttcaatgc
    59401 gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag ttcattaatg
    59461 agtatgtccg gcagagaggg ctctgccgga ttttacgact taatggcctg atgtgctgcg
    59521 cttaccagcg cctatgaaat aaatcgcaaa ttattttggt cagatacagc gtcgtatccg
    59581 ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta ccgtcagatc
    59641 gcgccacgct ttcggcctcg tccccagttt tccatccttc gcagctgtga gtaatttttc
    59701 tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg tcccattctt ttctgtctga
    59761 gtcaaagcca tagacaatga ctctgtcatc attaaaagca aacaggatcc gctccgcctg
    59821 accatcacta ttcagatctt gctcgaccag tacgcaggcg tctttttcaa tacaggtcat
    59881 cacgttatag cgatcctgga ttaaggccga ccagaatgtc gcatccggtt taacagaacc
    59941 aggggcaatt aacacatttt cggctaatac tttttccgat acctgttcct gaagatgttg
    60001 ctctccatct aacgccatca gcagatcccg cgcgcgtttc gggtctttca taaacccggc
    60061 atcgcttttc agcgactcaa gcgccgcacg tccatagcga ccgctctgtt cgagcatgta
    60121 gatagttacc tggtctggcg tgtttttgcc gctctgataa cgcgccatat gactgttcac
    60181 gctaatacgc atactgtcca gcaccggcga attaagaagc accagtatca ccaacaccaa
    60241 taacgaaacc gcaaggttca ctttgccctg aagaacaagg ggattttgcc cattacgcca
    60301 gacgatactg acaaaatacc ccagcgacca caccagtaac accagctccg ccagcgcgcc
    60361 ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc agccataacc cccaggtggc
    60421 gacgaacaca tacagcggag caaccagcaa agcggtttta atcaggcaac gcaacggccc
    60481 cgtccaggga agtgacgctt tttgcggatc gcggacaata gccattaaga tcaattgcaa
    60541 aaaggccagc gttaacagca acccggcggc ggagatgtgg cgagaaatcg cgctcaggcc
    60601 cgtaaacggc agggtgatga taaacatcag ggttagcaat gatactaacg gcagcaaccc
    60661 cgtggcgatt agcgtgaaca acttttgaat agagtcgatt aaacgtgact gtgtacgcgc
    60721 gaggatcacc gccagtgcgg taaccagacc aaatgtaaga tacataaacc agtcggttgc
    60781 aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta aacaactcac cccacagtaa
    60841 aagcaccagc cacgtcaggc cattggagag aaaaatcacc agtaatataa gtacgttatg
    60901 ccatactgac tggtaaaagt agctatagcg gctactgcca ttgcggatac gaaggctttg
    60961 ttgtatccac ggtagcaaca acattgccat caacagcaga tagcagccaa aatcccaaat
    61021 cgccttttca gctctccagg ggttcatgcc atcagtttgc cacttcagcc aaccgctcat
    61081 ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa aggcgcttct gtttaaaaga
    61141 gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc gcaggcacgc cataaacaat
    61201 ccaactgtga ttttttccgg caagccaggc gataagcagg tagcaaacca gcccctgaag
    61261 taatccggta gcaatcatcc cccagcgtgt tgccggtgaa agttcaacgt tatccatgtt
    61321 ggttatccag aatcaaaagg tgggttaatt atcgcatccg ggcagtagta ttttgctttt
    61381 ttcagaaaat aatcaaaaaa agttagcgtg gtgaatcgat actttaccgg ttgaatttgc
    61441 atcaatttca ttcaggaatg cgattccact cacaatattc ccgccatata aaccaagatt
    61501 taaccttttg agaacatttt ccacacctaa aatgctattt ctgcgataat agcaaccgtt
    61561 tcgtgacagg aatcacggag ttttttgtca aatatgaatt tctccagata cgtaaatcta
    61621 tgagccttgt cgcggttaac acccccaaaa agactttact attcaggcaa tacatattgg
    61681 ctaaggagca gtgaaatgcg cgttaacaat ggtttgaccc cgcaagaact cgaggcttat
    61741 ggtatcagtg acgtacatga tatcgtttac aacccaagct acgacctgct gtatcaggaa
    61801 gagctcgatc cgagcctgac aggttatgag cgcggggtgt taactaatct gggtgccgtt
    61861 gccgtcgata ccgggatctt caccggtcgt tcaccaaaag ataagtatat cgtccgtgac
    61921 gataccactc gcgatacttt ctggtgggca gacaaaggca aaggtaagaa cgacaacaaa
    61981 cctctctctc cggaaacctg gcagcatctg aaaggcctgg tgaccaggca gctttccggc
    62041 aaacgtctgt tcgttgtcga cgctttctgt ggtgcgaacc cggatactcg tctttccgtc
    62101 cgtttcatca ccgaagtggc ctggcaggcg cattttgtca aaaacatgtt tattcgcccg
    62161 agcgatgaag agctggcagg tttcaaacca gactttatcg ttatgaacgg cgcgaagtgc
    62221 actaacccgc agtggaaaga acagggtctg aactccgaaa acttcgtggc gtttaacctg
    62281 accgagcgca tgcagctgat tggcggcacc tggtacggcg gcgaaatgaa gaaagggatg
    62341 ttctcgatga tgaactacct gctgccgctg aaaggtatcg cttctatgca ctgctccgcc
    62401 aacgttggcg agaaaggcga tgttgcggtg ttcttcggcc tttccggcac cggtaaaacc
    62461 accctttcca ccgacccgaa acgccgcctg attggcgatg acgaacacgg ctgggacgat
    62521 gacggcgtgt ttaatttcga aggcggctgc tacgcaaaaa ctatcaagct gtcgaaagaa
    62581 gcggaacctg aaatctacaa cgctatccgt cgtgatgcgc tgctggaaaa cgtcaccgtg
    62641 cgtgaagatg gcactatcga ctttgatgat ggttcaaaaa ccgagaacac ccgcgtttct
    62701 tatccaatct atcacatcga taacatcgtt aagccggttt ccaaagcggg ccacgcgact
    62761 aaggttatct tcctgactgc tgatgctttc ggcgtgttgc cgccggtttc tcgcctgact
    62821 gccgatcaaa cccagtatca cttcctctct ggcttcaccg ccaaactggc cggtactgag
    62881 cgtggcatca ccgaaccgac gccaaccttc tccgcttgct tcggcgcggc gttcctgtcg
    62941 ctgcacccga ctcagtacgc agaagtgctg gtgaaacgta tgcaggcggc gggcgcgcag
    63001 gcttatctgg ttaacactgg ctggaacggc actggcaaac gtatctcgat taaagatacc
    63061 cgcgccatta tcgacgccat cctcaacggt tcactggata atgcagaaac cttcactctg
    63121 ccgatgttta acctggcgat cccaaccgaa ctgccgggcg tagacacgaa gattctcgat
    63181 ccgcgtaaca cctacgcttc tccggaacag tggcaggaaa aagccgaaac cctggcgaaa
    63241 ctgtttatcg acaacttcga taaatacacc gacacccctg cgggtgccgc gctggtagcg
    63301 gctggtccga aactgtaatg atttgaagct ggagaatatc tatccagtat cttatagaaa
    63361 gcaaaacggg aggcaccttc gcctcccgtt tatttaccct tcttttgtcg tgccctgcgc
    63421 ccgcgttacc ggcactggca gccaggcgcg aatggaaagc ccgccccgct cgctggtgcc
    63481 aagctccagc atcccgttat ggttatccac gatacgctgc acaattgcca gccctaatcc
    63541 cgtgccgcta atggtgcgcg cactgtcgcc gcggacaaac ggctggaaca ggtgcttacg
    63601 ttgttccggc gcaattcccg gaccgtcatc ttccacctgg aaccaggcgc gattcggctc
    63661 cgttccgctg ctgactttga tccagccatt gccataacgg gcggcgttga ccaccatatt
    63721 cgccaccgcg cgtttgatcg acagcgggtg cattttcact tcaatgctgc cggggtaaag
    63781 cgcggtttca atttcccgct catagccact ttcggcagca atcacctcac cgagcactgc
    63841 attaagatcc gccatttcca tcggcatctc ctgcccggtg cgcaggtagt cgataaactg
    63901 ctcaatgatg gcgttgcact cttcgatatc tttattgatc gattctgcca gatagccatc
    63961 ctgctcgctc atcatctcag tcgccaggcg aatacgcgtc agcggcgtgc gcaagtcgtg
    64021 acttaccccc gccatcagca gcgtgcggtc atccgccagt tgcttaacac cagccgccat
    64081 atggttaaag gcacgggtaa cggaacgcac ctccgaagcg ccatattcac gcagcggcgg
    64141 cggaataatc cctttaccaa cctgcaaggc tgcgtgttcg agatcgacca acggtcggtt
    64201 ctggatacga ataaacagcc acgccccgcc tatcgccaat agcataatcg ccagcgtata
    64261 gcggaacagc ggagagaaat cgccctgatg aatttcggtc agcggcacgc gtacccagat
    64321 attgggcgac agccaggttt tcagccagac gacaggcgaa cttttgttga cctcaacgcg
    64381 cacttccgtc gggccgccca gttgctgtgc catctgatgg cttaagaatt catagtgttg
    64441 cgcccaacgc agacctgcct cttcggcagc ctcgttggag tagagagaga tccccagctc
    64501 acggtagatc tcccgacgga aagcgggagg cacaaccaac tgcgtgccgt cctccagttg
    64561 cagtttatcg gtcatcaaca tacgcacttc gtacgcgagg actttattaa actgctggag
    64621 gctcggcaaa atcgcgaagt tcagcaccac cagataagtc gtcaccaggc tggcgaacag
    64681 caaggtgacg atgagcaata acgtacgggc aaatgaactt cgtggcgaga agcgcaatcg
    64741 cctcatgctt tagagccgtc cggtacaaag acgtagccca ggccccaaac ggtctgaatg
    64801 taacgcggat gcgctggatc ttcttctacc atgcggcgca gacgcgaaat ctgcacgtcg
    64861 atggagcgtt ccattgcgga atattcacga ccacgggcaa ggttcatcag cttatcgcgg
    64921 gagagcggct cacgcggatg gctgaccagt gccttcagta ccgcaaactc accgctggtg
    64981 agcggcatcg gctcatcttc gcggaacatt tcgcgcgtac cgaggttaag tttgaactta
    65041 ccgaaagcaa ttaccgcctc ttcctgtgac ggtgcgcctg gcagttcgtt cgcctgacga
    65101 cgcagcaccg cacgaatacg ggccagcagt tcacgcgggt taaacggttt tggaatgtag
    65161 tcgtcagcgc caatctccag gcctacgata cggtccactt cttccccttt cgccgtcacc
    65221 ataatgatcg gcatcgggtt gctctgacta cgaagacgtc ggcaaatcga caagccatct
    65281 tcaccaggta acattaaatc cagtaccata agatggaaag attcacgagt cagcaggcga
    65341 tccatctgtt ctgcattagc gacgcttcga acctggaagc cttgttcggt gagataacgt
    65401 tccagcagcg cacgcaggcg catgtcgtca tcgaccacca gaatcttgta gttctcttgc
    65461 attgtttgta ctcccaaagg ttcgcaacaa tttgtaagcg tgtattctta aaaaagctca
    65521 cgttcgtcac cagctaaatc tggtatgaat ttcagcctaa attgttacaa agcatattaa
    65581 acagcagctt aagtatacaa tttattcggc gaaacattat tgattctgtt gatatgatca
    65641 cgttataccc aatgtgcgca ttatcaaaca gacaaaggga atcaacgaga tgaaaacgcc
    65701 cctggttacc cgggaagggt atgaaaaact caaacaagag cttaattatc tctggcgtga
    65761 agaacgcccg gaggtcacaa aaaaggtgac ctgggccgca agtctgggcg accgcagcga
    65821 aaatgctgac tatcagtata ataaaaagcg tctgcgtgaa atcgaccgtc gcgtgcgcta
    65881 tctcactaaa tgcctggaaa atctcaaaat tgtcgattac tcccctcagc aggaaggcaa
    65941 agtctttttt ggcgcgtggg tggagattga aaacgacgat ggcgtgactc accgtttccg
    66001 tattgtcggc tacgatgaaa tttttggccg taaagattac atctctatcg attccccgat
    66061 ggcccgcgca ttgctgaaaa aagaagtcgg cgatctggcg gtggtgaata cccctgccgg
    66121 ggaagcgaac tggtatgtta atgctatcga gtacgtgaaa ccgtaaggaa gagtcttaac
    66181 ctcctgccga tggctggcat ttttgccagc cagtccgtat aactatcccc tgatttttga
    66241 tccgaaaaga tgaactcaaa ccatgatgaa tgattcgttc tgccgcatta ttgcgggtga
    66301 aattcaggcg cgcccggaac aggttgacgc tgccgttcgc ctgcttgatg aagggaatac
    66361 cgtgccgttt atcgcacgtt atcgtaagga aatcaccggc ggtctggatg acacgcagct
    66421 gcgtaatctg gaaacgcgtc tgagctatct gcgcgagctg gaagagagac gtcaggcgat
    66481 cctcaaatcc atttccgagc aaggcaaact caccgatgat ctggcgaagg ccatcaacgc
    66541 caccctaagc aaaaccgaac tcgaagacct ctacctgccc tacaaaccta aacgccgcac
    66601 ccgcgggcaa atcgccattg aagcagggct tgagccgttg gctgacctgc tgtggagcga
    66661 tccgtcacac acgccagaag tcgccgctgc acaatatgtt gatgccgata aaggcgtggc
    66721 agataccaaa gccgcgctgg atggcgcgcg ctatatcctg atggaacggt ttgccgaaga
    66781 tgccgcgctg ctggcgaaag tgcgtgatta tctgtggaag aacgcgcatt tggtttctac
    66841 ggtggtgagc ggtaaagaag aggaaggggc gaaattccgc gactatttcg atcatcacga
    66901 accgttgtcc acggtgcctt ctcaccgcgc gctggcgatg ttccgtgggc gtaacgaagg
    66961 cgtactccag ctttcgctga atgccgatcc gcagttcgat gagccgccca aagagagcta
    67021 ttgcgagcaa atcatcatgg atcaccttgg cctgcgcctg aacaatgccc cggcggatag
    67081 ctggcgcaaa agcgtagtga gctggacctg gcgcatcaag gtgctgatgc atctggaaac
    67141 cgaactgatg ggtaccgtgc gcgaacgcgc ggaagatgaa gcaatcaacg tctttgcccg
    67201 taacctgcac gatctgctga tggcggcccc tgccggactg cgtgcaacga tgggcctcga
    67261 tccgggtctg cgtaccgggg taaaagtggc ggtggtcgat gccactggca aactggtggc
    67321 gaccgatacc atttacccgc acaccggaca agccgcaaaa gcagcgatga ccgttgctgc
    67381 cttgtgtgaa aaacataacg ttgaactggt ggcgatcggc aacggtacag cttcccgcga
    67441 aactgaacgt ttctatctcg acgtgcagaa gcagttcccg aaagtgaccg cacagaaagt
    67501 gatcgtcagc gaagctggcg cgtcggttta ctcagcttcc gagctggcag cgcaggagtt
    67561 cccggatctc gacgtttcgc tgcgtggtgc ggtatctatc gcccgccgtt tgcaggatcc
    67621 gctggcggag ctggtgaaaa tcgatccgaa atctatcggc gtaggtcagt atcagcatga
    67681 cgtcagccag acgcaactgg cccgcaaact ggatgcagta gtagaagact gcgtaaacgg
    67741 cgttggcgtc gatctcaaca ccgcttctgt tccgctatta acccgcgtgg cgggcctgac
    67801 gcgcatgatg gcgcaaaaca tcgtggcctg gcgcgatgag aacggccagt tccagaaccg
    67861 tcagcaactg ctgaaagtca gccgtctggg gccgaaagcc ttcgagcagt gcgcgggctt
    67921 cctgcgcatt aaccacggtg ataacccgct ggacgcctct accgttcatc cggaagccta
    67981 tccggtggtg gaacgcattc tggcagcaac acagcaggca ctgaaagatc tgatgggtaa
    68041 cagcagcgaa ctgcgtaacc tgaaagcgtc tgactttact gatgaaaaat tcggtgtgcc
    68101 gacagtaact gacatcatca aagagctgga aaaaccgggt cgcgatccgc gtccggaatt
    68161 taaaaccgct cagtttgccg atggcgtcga gacaatgaac gacctgcaac cgggtatgat
    68221 cctcgaaggc gcagtgacca acgtcaccaa ctttggcgcg tttgtcgata ttggcgtgca
    68281 tcaggacggc ctggttcaca tctcttcatt gtcgaacaag tttgtggaag atccgcatac
    68341 cgtggtgaaa gcgggcgaca ttgtgaaggt gaaagtgctg gaagtggatc ttcagcgtaa
    68401 acgtatcgcc ctgactatgc gtctggacga gcagcctggc gaaaccaact ctcgtcgcgg
    68461 cggcggtaat gaacgcccgc aaaacaaccg cccggcagcc aaaccacgcg gtcgtgaagc
    68521 gcagcctgcc ggtaatagcg cgatgatgga tgcgctggcg gcagcgatgg gtaaaaaacg
    68581 ttaaacgccc ggactggccc ctaaacctgc gcgataaggc gttcacgccg catctggcaa
    68641 cgaacgcttt gcctgacata aaagtgccgg agaatatctc cggcactttt attccacagc
    68701 caaactcaca atatactccg ttaatattta tcacttcatt aacaactgaa accttaatta
    68761 aacattagcc agtccgggta actcactatt cgaattatat tttcgctgcg atataacctt
    68821 gagccacatc aacattgagt cagattatta ttcaaaccaa cattcgtaca cattttaagt
    68881 attgctgata gaaaccattc tcattatcat tgtgttgttg attatttaat ctctcctttg
    68941 ttggcaaatc atctggtctc atgtcgcggt caaacgcccc atgaggtagt tatccagtta
    69001 atgagaaaca agtaggcacc tatgcaatac actccagata ctgcgtggaa aatcactggc
    69061 ttttcccgtg aaatcagccc ggcatatcgc caaaaactgc tttctctagg catgttacct
    69121 ggctcctctt ttgatgtggt gcgcgtcgct ccactcggcg accccattca tatcgaaacc
    69181 cgtcgtgtga gcctggtatt acgcaaaaaa gatctggcct tattagaagt ggaagcggtt
    69241 tcctgttaat acggtgataa caacaatgaa aaaattaacc attggcttaa ttggtaatcc
    69301 aaattctggc aagacaacct tatttaacca gctcactggc gcacgtcagc gtgtaggtaa
    69361 ctgggctggc gttaccgtcg aacgtaaaga agggcaattc tccaccaccg atcatcaggt
    69421 cacgctggtg gacctgcccg gcacctattc tctgaccacc atctcatcgc agacctcgct
    69481 cgatgagcaa atcgcctgtc actacatttt gagtggcgac gccgacctgc tgattaacgt
    69541 ggtggatgcg tctaaccttg agcgtaacct gtacctgacg ctgcaactgc tggaactcgg
    69601 cattccctgc atcgtggcgc tgaacatgct cgacattgcc gagaagcaaa atattcgtat
    69661 tgaaattgat gctctatcgg cacgtcttgg ctgcccggtg atcccgctgg tttcaacccg
    69721 tggtcgcggt attgaagcgc tcaagctggc gattgatcgc tataaagcta acgagaatgt
    69781 ggaactggtg cattacgcac agccgctact caacgaagca gattcactgg caaaagtgat
    69841 gccttccgac atcccgctga aacaacgtcg ctggctgggc ctgcaaatgc tggaaggcga
    69901 tatctacagc cgcgcctacg ccggtgaagc gtcgcagcat ctggatgcca ccctcgcccg
    69961 tctgcgtaat gaaatggatg atccggcgct gcacattgcc gatgcgcgtt accagtgcat
    70021 tgctgccatc tgtgatgtgg taagcaacac cctgacggca gaacccagcc gtttcaccac
    70081 tgcggtagat aaaatcgtgc tcaaccgttt cctcggtctg ccgattttcc tctttgtgat
    70141 gtacctgatg ttcctgctgg ctatcaacat cggcggggcg ttacagccgc tgtttgacgt
    70201 cggctccgtg gcgctatttg tgcatggtat tcaatggatt ggctacacgc tccacttccc
    70261 ggactggctg actatcttcc tcgcccaggg catgggtggc ggtattaaca ccgtgctgcc
    70321 actggtgccg cagatcggca tgatgtacct gttcctctcc ttccttgagg actccggcta
    70381 tatggcgcgt gcagcgtttg tgatggaccg cctgatgcag gcgctgggct tgccggggaa
    70441 atcctttgtg ccgctgatcg tcggtttcgg ttgtaacgta ccgtcggtaa tgggcgcacg
    70501 tacgcttgat gcaccgcgtg aacgtctgat gaccatcatg atggcaccgt ttatgtcctg
    70561 cggcgcgcgt ctggctatct tcgcagtatt cgcggctgcc ttcttcgggc agaacggtgc
    70621 cctggcggtt ttctcgctgt atatgctcgg tatcgtgatg gcggtgctga ctggcctgat
    70681 gctcaagtac accatcatgc gcggtgaagc aacgccgttt gtcatggagc tgccggtata
    70741 ccatgtacca cacgttaaaa gcctgattat ccagacctgg cagcgtctga agggcttcgt
    70801 tctgcgtgct ggtaaagtga ttatcatcgt cagcattttc ctgagcgctt tcaacagctt
    70861 ctcgttgagc gggaaaatcg tcgataacat caacgactcg gcgctggctt ccgtcagccg
    70921 ggtgataacc cctgtcttca agccgattgg cgtacatgaa gataactggc aggcaacggt
    70981 tggcctgttt acgggcgcaa tggcaaaaga agtggtggtg ggtacgctca acaccctcta
    71041 caccgcagaa aatattcagg acgaagagtt caatccggcg gaattcaacc tcggtgaaga
    71101 gctgttcagt gcggtagatg aaacctggca gagcctgaaa gacaccttca gccttagcgt
    71161 actgatgaac cccattgaag ctagcaaagg cgacggtgaa atgggtaccg gggcgatggg
    71221 cgtgatggat cagaaattcg gtagcgcagc tgccgcatac agctacctga ttttcgtcct
    71281 gctgtatgta ccatgtatct cggtgatggg agccatcgcc cgtgaatcaa gccgtggctg
    71341 gatgggcttc tccatcctgt gggggctgaa tatcgcttac tcactggcaa cattgttcta
    71401 tcaggtcgcc agctacagtc agcacccaac ttacagcctg gtgtgcattc tggcggtaat
    71461 cctgtttaac atcgtagtta tcggtctgct gcgccgcgcg cgtagccggg tggatatcga
    71521 actgctggca acccgcaagt cggtaagcag ttgctgcgca gccagcacca ccggtgattg
    71581 ccattaatgg cttcacttat tcaggtacgt gatttgctgg cgttacgggg ccgtatggaa
    71641 gcgacccaga taagccagac attgaacact ccgcagccaa tgattaacgc catgctgcaa
    71701 cagctggaaa gcatgggcaa agccgtgcgg attcaggaag aacctgacgg ctgcctctct
    71761 ggcagttgta aaagctgccc ggaaggcaaa gcctgtctgc gcgagtggtg ggcgctgcgt
    71821 taaccatact ccatcgcctg ttttgaaaag cccggtatgc gtttgcatcc gggctttttt
    71881 gcgtgcggct ttccataaaa atgcaattct tgcagcgcaa cgtaagttcc tctgaaagcg
    71941 tctcgcaggg atgaaaactc gctaatgcac agatgtggag tggcgcgtag agtcgcggca
    72001 ttcaaacaac aggtgaagga acgccatgag taaaaagcag agttccaccc cacatgatgc
    72061 gctgttcaaa ctctttttac gccaaccgga gacggcacgc gattttcttg cgtttcattt
    72121 accggcaccc attcacgcgc tctgtgatat gaaaaccctc aagctggagt cgagcagctt
    72181 tattgatgac gatctgcgtg aaagctattc cgatgtgctg tggtcggtga aaacggaaca
    72241 aggaccagga tacatctatt gtctgattga acatcaaagc acctcaaaca aactgatcgc
    72301 atttcgcatg atgcgttacg ctattgccgc aatgcaaaat caccttgatg ctggatacaa
    72361 aacgttgccg atggtggtgc cattgttgtt ttaccacggt attgaaagcc cctatcccta
    72421 ttcgctgtgt tggctggatt gtttcgccga tcccaaactg gcaaggcagc tttatgcctc
    72481 cgcatttccg ctgattgatg tcaccgtcat gcctgatgat gaaatcatgc agcaccgacg
    72541 tatggcgctg ctggagttaa ttcaaaaaca tattcgtcaa cgcgatctga tggggctggt
    72601 agagcaaatg gcctgcttat taagtagtgg atacgctaat gacagacaaa tcaaagggct
    72661 gtttaattac atactgcaaa ctggcgacgc tgtacgtttt aacgatttta tcgacggcgt
    72721 tgccgaacgt tcaccgaaac acaaggagag tttaatgact attgcggaaa gattgcggca
    72781 ggagggggaa caatccaaag ccctgcatat agccaaaata atgcttgaat ccggagtccc
    72841 tcttgcagac atcatgcgat ttaccgggct gtcagaagaa gagttggctg cggcgagtca
    72901 gtagatactc taccctgctt aagtgctact ttgtgctatc ttgcatgtaa cacaaagtag
    72961 cacttaggga actgatatgc agacagtgaa attacggcaa caaggaggag ccatgatcgt
    73021 tactatccca cgagatcttg ccatcgatct tggctggtca ccgggcacag agcttaccgt
    73081 ggagaaaaaa ggcgatagcg ttaatttgcg cgccacagag cacaaaccgc gtgggcgtct
    73141 taccgtcgcg cagttgttga gccaaatcga tgaaagcgaa attacggagc taaatcagtc
    73201 cacagaagga tgggctgaag ggaaaaaagg taatgaagca tggtaaaggc acggacgcca
    73261 catcgtggtg agatctggta ttttaaccct gatccggttg ccgggcatga acttcagggg
    73321 ccacattatt gcattgtggt aacggacaaa aaactcaaca atgttttaaa agttgctatg
    73381 tgctgcccga tttcaacagg ggcaaatgca gcacgttcca caggggtgac ggtgaacgtc
    73441 ctcccctgtg atacgcaaac cggtaacctg catggcgttg tactttgtca ccagctaaaa
    73501 gccgtcgatc ttattgcccg tggcgctaaa tttcataccg ttgccgatga aaaattgatt
    73561 agtgaagtta tcagtaaact ggtgaattta atcgacccac aataattagc tctcacttac
    73621 caccaacacc ctctgcttca acgccaccag caggtgacaa aactcgaccg gatgcgaaat
    73681 aaacggcgca tgggccgctt tggcgaagat atatgattcg ctgtgaggcc aaagtttatc
    73741 cagcatcggc accactttgc gcggcaccag accgtcgaga tagccataca atcgcaaaaa
    73801 cggcatggac acgttttgca gcggctgacg gagatcgacc gttttcagga tttccagccc
    73861 gccattaagc acgtcaacct ccggcatcgg taacgccaga acggttttct tcaacgcccg
    73921 cgcatcctgg cgcgccgttt cagtccccat ggtttgtaac gccaggaacc gctccactgt
    73981 acgctgaaaa tcatcactga gttgctgctg aaatcccgcc agcacgtccg gttttatccc
    74041 cggccactcg tcacgagcac taaaataagg tgacgacgcc acggtgacca gcgcctgaac
    74101 acgctcggga tgggttaacg caatctggct tgccaccagc ccgcccagac tccagcctaa
    74161 ccaaatggct ttatcaggtg cctgttgcag cacggcttcg gccatatcag caagtgacag
    74221 cgcaccaaat ccccggctac gcccgaagcc gggcaggtca acaaggtgca gcgtaaaatg
    74281 cgagctaagt tcctcgtcaa tgcaacgcca cacttcggca ttcagtcccc atccgtgcag
    74341 cagcacaaga tgaacattcc cctgaccttt ggtctgccac cagatgttat tcatccgcta
    74401 ttgttctctt ttgacttaca aggatgaaca tatgctaaca gtaccgggat tatgctggct
    74461 atgccgaatg ccactggcgt taggtcattg ggggatttgt tcggtctgtt cacgcgccac
    74521 ccgcacagat aaaacgttat gcccacaatg tggattaccc gccacacact cccatcttcc
    74581 ctgcggtcgc tgcctgcaaa aaccgccgcc ctggcaaaga ctggtcacgg ttgccgacta
    74641 tgcgccgccg ttaagtccgc ttatccacca gcttaaattt tcccggcgca gtgaaatcgc
    74701 cagcgccctg tcacgtctgt tactattgga agtcttacac gctcgtcgca ccaccgggtt
    74761 gcaattgccg gatcgcatca tcagcgttcc gttatggcag cggcgtcact ggcgtcgggg
    74821 atttaatcag agcgatttgc tgtgtcagcc gttatcacgc tggttgcact gccaatggga
    74881 tagcgaagcc gtcacacgta cacgggccac tgcgacccag cattttctca gtgcccggct
    74941 gcgcaagcgc aacctgaaaa atgcctttcg tcttgaattg cccgtgcaag gtcgccatat
    75001 ggtgattgtg gatgatgtcg ttaccaccgg aagtaccgtc gcagagattg cgcagttgct
    75061 tttacgcaat ggtgcggcga ctgtccaggt ctggtgcctt tgtcgaacct tgtagagcct
    75121 cgatgatggg cgtattataa ccaactaaaa tagtcaacta ttaggccatt actatgatcc
    75181 gtatttccga tgctgcacaa gcgcactttg ccaaactgct ggcaaatcag gaagaaggga
    75241 cacaaatccg cgtatttgtg attaaccctg gcacgcctaa cgctgaatgt ggtgtttctt
    75301 attgtccgcc ggatgccgtg gaagccaccg acactgccct gaaatttgac ctgctgaccg
    75361 cgtatgttga tgagttaagc gcaccatacc tggaagatgc agagatcgac tttgttaccg
    75421 accagttggg ttctcagtta acgctgaaag ccccgaacgc caaaatgcgt aaagtggcag
    75481 acgatgcacc gctgatggag cgcgtggagt atatgctgca gtcgcagatc aacccacagc
    75541 ttgctggtca cggtggtcgc gtttcgctga tggaaatcac cgaagacggt tacgccattc
    75601 tgcaatttgg cggcggctgt aacggttgtt ccatggtcga tgtgacgctg aaagaaggga
    75661 tcgagaagca gctgctgaac gaattcccgg agctgaaagg tgtgcgcgat ctcaccgaac
    75721 accagcgcgg cgaacactcc tactactaag ttatcccctc ttttgtagat tgcccgatgc
    75781 gacgctaaag cgtcatttcg ggcctacgtt gaacatgtgc cagacgttgg cacagcattg
    75841 ctttggcaaa tccctccctt cgtttccccg cgataatatg accaacctct cataatttaa
    75901 atttaccccg ctctggtgat tctcaaacgc cagatgttac ccgtatcatt cacatgggta
    75961 ccaaacatac tcctgacatc tgactacaat aattagtttt agtgggtatc agtcgtggtg
    76021 ccgcaatatc tctgttcccg attgggataa ttagagtttg tcgtcagaaa attgacatta
    76081 cccataacaa atgaaaggcc aggtaaatca tgccattagt cattgttgct atcggtgtaa
    76141 tcttgttgtt gctcctgatg atccgcttca aaatgaacgg cttcatcgct ctcgtcctcg
    76201 tggcgcttgc tgttggatta atgcaaggaa tgccgctgga taaagttatt ggctccatca
    76261 aagccggtgt cggcgggacg ctcggtagcc ttgccctgat catgggtttt ggcgcaatgc
    76321 tggcaaactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa tttggtaaaa
    76381 agcacatcca gtgggcggtg gtgttaaccg gttttaccgt tggttttgcc ctgttctatg
    76441 aagtgggctt cgtgctgatg ctgccgctgg tgtttaccat cgcggcttcc gcgaatattc
    76501 cattgctgta tgttggtgta ccaatggcgg ctgcactgtc tgtgacccac ggcttcctgc
    76561 caccgcatcc gggcccgact gcgattgcca ccattttcaa tgccgatatg ggtaaaaccc
    76621 tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg gtttacgctc
    76681 gcgtgttgaa aggtatcgat aagccaatcc cggaaggtct ctacagcgcg aaaaccttca
    76741 gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg ccggttgtac
    76801 tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct ttcctgccgg
    76861 tagcggagtt cctcggtgac ccggtaatgg caacgctgat tgccgtgctg attgcgatgt
    76921 tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg ctggtttctt
    76981 ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg ttcaagcagg
    77041 tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa accaacattt
    77101 ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg ggttccgcaa
    77161 ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg acgggtgtta
    77221 gcccagagct gatggttatt gcggtgggtt ccggtagtgt gattttctct catgtgaacg
    77281 atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag accatcaaat
    77341 cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt ctgctgctga
    77401 atatggtgat ttgataacac tgactgccgg atgcatcgca tccgacatca accgcactct
    77461 acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac ttgttcacgc
    77521 catcatcggc aaacatcgac tcaagcgttg cggaaagctt gcgtcgccag tttttatact
    77581 ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct tccggctgta
    77641 gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg ttcagcgtcg
    77701 tcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa ccatatttat
    77761 gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga ccgcgcagta
    77821 ccacttcatc cggatacagc cccagggttt tgcccagcgt tagatccccg ctctcccagt
    77881 aaccacgcag cgttggcagg tcatgtgtcg ccgcaaccgc catcgactgc tccggatacg
    77941 ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact ttgtaagagt
    78001 acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg agatcttcac
    78061 caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc agatcatcca
    78121 ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc caccacaaac
    78181 gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata ttggcacgca
    78241 acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc attggcggta
    78301 atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc gatgctttca
    78361 ggcaatatag ttcacggtca caccaggttt ccgccccacc ttccgctacg ccaaccgcca
    78421 gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag caggcggcaa
    78481 actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatca cgatgttctt
    78541 cgcagaactg acgcacttct ggtgaatcca cgttcttata catctctggc catgcaggcc
    78601 agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca aaggctgcct
    78661 gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggcc atctgctcat
    78721 catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt agggcggtaa
    78781 ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt tgcgtggtcg
    78841 gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg gcgttaacgt
    78901 cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg gcgctctccg
    78961 gatttgccgg atagagcgca tgaatcgggt tcaggccaat gaacgacccg ccacgttttg
    79021 ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag tttttttccg
    79081 atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc agcaaagcct
    79141 gcggttcgta acaacgtttc ggggcgacaa tcacccggca atgcgcgcgc tggtcgtcct
    79201 gggtgagtgt tagcgtgtga taaccttccg gcagcttcgt cggtagattg aacgctttgc
    79261 cccccgttac atggcctttg tactgcgttc cttcttcggt ggtcagctgc cagctatatt
    79321 cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc atgacattcg
    79381 ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca agcaaacgcc
    79441 gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa ttggggctaa
    79501 tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt ccttagcgtt
    79561 ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta aacataccgc
    79621 agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg tacagcacat
    79681 ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga tacggatcgc
    79741 cgccctgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg ccgtcgctgt
    79801 atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc ttccgccatt
    79861 tcaccgggtc gtagcctttg gccagaatag ccttcacttg ttccacggta tgaccaaaaa
    79921 taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca tccagcgtac
    79981 cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa gcttctttac
    80041 ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc gccgccgaaa
    80101 cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga tcgttgttga
    80161 tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag tagcccggtg
    80221 ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcagg ttttcacgaa
    80281 tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg tactcatgca
    80341 gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaataccg gtacgaactt
    80401 tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat tgctgacgga
    80461 atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg ttagcccact
    80521 ctttttgcag tgatttatcc aacagagccg ccagtgcagg gttgcactgt ttgatccagc
    80581 gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc tggtgatatt
    80641 ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg ttcaccgcga
    80701 aaccgccaac cacacacagg ttcgccatat gcacttgttt atcgtgcacc accgccagtt
    80761 tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta aagcgagtat
    80821 taatttcgtt aataatctgc atatggcgcg gcagtaagcc tttcaccagt ttcacatccc
    80881 agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt ttgctggtga
    80941 tggcccaggc gtcatcccag ctcatctggt gctcatcggt cagcacgcgc agcagttctg
    81001 gaatcgcgat agttggatgg gtatcgttca gctgaataac ttcgtaatcc gccagttcgt
    81061 gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa caggcacact
    81121 ggaagtattg ctgcatcagg cgcagctttt taccggcagt atggttgtcg tttggataga
    81181 gaactttggt cagtttttcc gcattgatgc cttgctgttc ggcgcgcagg aaatcaccgt
    81241 cgttaaattt agttagatca aacggatgcg catgcgtcgc ctgccacaga cgcagcggct
    81301 gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg gtaatggtaa
    81361 actccggctc ccagcgtccg tctttcatca ctttaccgcc aatccctacc tgcacatcca
    81421 gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc ggcgcttcaa
    81481 cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc agaccgtaac
    81541 ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc agacgtccca
    81601 gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtcagtc aggttgatgt
    81661 cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac aggttgttgc
    81721 ccgtcagacg accaatcaaa aactccattg agatgtagtt aacatgtcgc tgattcgcca
    81781 ccggtttggc gaatggctga gcacgcagca tttcggccag tgcttcactc actgccagcc
    81841 accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc cactgacgtg
    81901 aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga gttccacttt
    81961 tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg accttcctca
    82021 tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg tgatctcgac
    82081 cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgccatac agcgcagaaa
    82141 atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga aggtaactct
    82201 ataaattaag taaaggagtg aaacagtttc acaagtaaaa tatccagtgt gctccatctc
    82261 attcttaata gatttattaa gatcatcttt ttagatggca ctttcatcag gaatgaagaa
    82321 gaaacccttg cttaaatgaa tctgat
//
LOCUS       DADLTF010000003        78957 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.003, whole genome shotgun sequence.
ACCESSION   DADLTF010000003 DADLTF010000000
VERSION     DADLTF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 78957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.003"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1592)
                     /locus_tag="JN749_000188"
     CDS             complement(<1..1592)
                     /locus_tag="JN749_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7366731.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDKNEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="JN749_000189"
     CDS             complement(1576..1932)
                     /locus_tag="JN749_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7366732.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="JN749_000190"
     CDS             complement(2055..2237)
                     /locus_tag="JN749_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366733.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="JN749_000191"
     CDS             complement(2221..2661)
                     /locus_tag="JN749_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7366734.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="JN749_000192"
     CDS             complement(2661..2957)
                     /locus_tag="JN749_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7366735.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="JN749_000193"
     CDS             complement(2954..3292)
                     /locus_tag="JN749_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7366736.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="JN749_000194"
     CDS             complement(3289..4500)
                     /locus_tag="JN749_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7366737.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="JN749_000195"
     CDS             complement(4502..5074)
                     /locus_tag="JN749_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7366738.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="JN749_000196"
     CDS             complement(5114..6271)
                     /locus_tag="JN749_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7366739.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6571..6873)
                     /locus_tag="JN749_000197"
     CDS             complement(6571..6873)
                     /locus_tag="JN749_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707048.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366740.1"
                     /translation="MEHYWLFVTVRHSYYLKQTDTTEENAMPMKFDEILKQRDKYHAD
                     NMETMNITDYRAFLETGALIEKDHHGFVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(6937..7098)
                     /locus_tag="JN749_000198"
     CDS             complement(6937..7098)
                     /locus_tag="JN749_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7366741.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(7108..7413)
                     /locus_tag="JN749_000199"
     CDS             complement(7108..7413)
                     /locus_tag="JN749_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366742.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7410..7688)
                     /locus_tag="JN749_000200"
     CDS             complement(7410..7688)
                     /locus_tag="JN749_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366743.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7968..9716)
                     /locus_tag="JN749_000201"
     CDS             complement(7968..9716)
                     /locus_tag="JN749_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7366744.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9713..10012)
                     /locus_tag="JN749_000202"
     CDS             complement(9713..10012)
                     /locus_tag="JN749_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7366745.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(10018..10251)
                     /locus_tag="JN749_000203"
     CDS             complement(10018..10251)
                     /locus_tag="JN749_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366746.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(10253..10473)
                     /locus_tag="JN749_000204"
                     /pseudo
     CDS             complement(10253..10473)
                     /locus_tag="JN749_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10466..10687)
                     /locus_tag="JN749_000205"
     CDS             complement(10466..10687)
                     /locus_tag="JN749_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366747.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10677..10877)
                     /locus_tag="JN749_000206"
     CDS             complement(10677..10877)
                     /locus_tag="JN749_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HAY7366748.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10886..11632)
                     /locus_tag="JN749_000207"
     CDS             complement(10886..11632)
                     /locus_tag="JN749_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366749.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11690..11878)
                     /locus_tag="JN749_000208"
     CDS             complement(11690..11878)
                     /locus_tag="JN749_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7366750.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(12283..13512)
                     /locus_tag="JN749_000209"
     CDS             complement(12283..13512)
                     /locus_tag="JN749_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7366751.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13761..14882
                     /locus_tag="JN749_000210"
     CDS             13761..14882
                     /locus_tag="JN749_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAY7366752.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14931..16157)
                     /gene="pepT"
                     /locus_tag="JN749_000211"
     CDS             complement(14931..16157)
                     /gene="pepT"
                     /locus_tag="JN749_000211"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAY7366753.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16407..17543
                     /gene="potA"
                     /locus_tag="JN749_000212"
     CDS             16407..17543
                     /gene="potA"
                     /locus_tag="JN749_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HAY7366754.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17527..18384
                     /gene="potB"
                     /locus_tag="JN749_000213"
     CDS             17527..18384
                     /gene="potB"
                     /locus_tag="JN749_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HAY7366755.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            18381..19175
                     /gene="potC"
                     /locus_tag="JN749_000214"
     CDS             18381..19175
                     /gene="potC"
                     /locus_tag="JN749_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HAY7366756.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            19172..20218
                     /gene="potD"
                     /locus_tag="JN749_000215"
     CDS             19172..20218
                     /gene="potD"
                     /locus_tag="JN749_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HAY7366757.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(20374..21195)
                     /gene="cobB"
                     /locus_tag="JN749_000216"
     CDS             complement(20374..21195)
                     /gene="cobB"
                     /locus_tag="JN749_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAY7366758.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCHFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(21211..22122)
                     /gene="nagK"
                     /locus_tag="JN749_000217"
     CDS             complement(21211..22122)
                     /gene="nagK"
                     /locus_tag="JN749_000217"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HAY7366759.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(22151..23395)
                     /gene="lolE"
                     /locus_tag="JN749_000218"
     CDS             complement(22151..23395)
                     /gene="lolE"
                     /locus_tag="JN749_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HAY7366760.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23395..24096)
                     /gene="lolD"
                     /locus_tag="JN749_000219"
     CDS             complement(23395..24096)
                     /gene="lolD"
                     /locus_tag="JN749_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HAY7366761.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(24089..25288)
                     /gene="lolC"
                     /locus_tag="JN749_000220"
     CDS             complement(24089..25288)
                     /gene="lolC"
                     /locus_tag="JN749_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HAY7366762.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25550..26596
                     /locus_tag="JN749_000221"
     CDS             25550..26596
                     /locus_tag="JN749_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7366763.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26724..30170
                     /gene="mfd"
                     /locus_tag="JN749_000222"
     CDS             26724..30170
                     /gene="mfd"
                     /locus_tag="JN749_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAY7366764.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            30313..31275
                     /gene="ldtC"
                     /locus_tag="JN749_000223"
                     /pseudo
     CDS             30313..31275
                     /gene="ldtC"
                     /locus_tag="JN749_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(31358..31615)
                     /gene="bhsA"
                     /locus_tag="JN749_000224"
     CDS             complement(31358..31615)
                     /gene="bhsA"
                     /locus_tag="JN749_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HAY7366765.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31856..32488
                     /gene="comR"
                     /locus_tag="JN749_000225"
     CDS             31856..32488
                     /gene="comR"
                     /locus_tag="JN749_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HAY7366766.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32550..33089)
                     /locus_tag="JN749_000226"
     CDS             complement(32550..33089)
                     /locus_tag="JN749_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7366767.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(33316..34620)
                     /locus_tag="JN749_000227"
     CDS             complement(33316..34620)
                     /locus_tag="JN749_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7366768.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(35021..35563)
                     /locus_tag="JN749_000228"
     CDS             complement(35021..35563)
                     /locus_tag="JN749_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7366769.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35586..36611)
                     /gene="nagZ"
                     /locus_tag="JN749_000229"
     CDS             complement(35586..36611)
                     /gene="nagZ"
                     /locus_tag="JN749_000229"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAY7366770.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36622..37446)
                     /gene="thiK"
                     /locus_tag="JN749_000230"
     CDS             complement(36622..37446)
                     /gene="thiK"
                     /locus_tag="JN749_000230"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HAY7366771.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37427..38068)
                     /gene="lpoB"
                     /locus_tag="JN749_000231"
     CDS             complement(37427..38068)
                     /gene="lpoB"
                     /locus_tag="JN749_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HAY7366772.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(38083..38460)
                     /locus_tag="JN749_000232"
     CDS             complement(38083..38460)
                     /locus_tag="JN749_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HAY7366773.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38463..38822)
                     /gene="hinT"
                     /locus_tag="JN749_000233"
     CDS             complement(38463..38822)
                     /gene="hinT"
                     /locus_tag="JN749_000233"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HAY7366774.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            39156..41345
                     /gene="fhuE"
                     /locus_tag="JN749_000234"
                     /pseudo
     CDS             39156..41345
                     /gene="fhuE"
                     /locus_tag="JN749_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41405..42838)
                     /gene="ptsG"
                     /locus_tag="JN749_000235"
     CDS             complement(41405..42838)
                     /gene="ptsG"
                     /locus_tag="JN749_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HAY7366775.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(43133..43930)
                     /locus_tag="JN749_000236"
     CDS             complement(43133..43930)
                     /locus_tag="JN749_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7366776.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43941..44945)
                     /gene="holB"
                     /locus_tag="JN749_000237"
     CDS             complement(43941..44945)
                     /gene="holB"
                     /locus_tag="JN749_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAY7366777.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAALVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44942..45583)
                     /locus_tag="JN749_000238"
     CDS             complement(44942..45583)
                     /locus_tag="JN749_000238"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAY7366778.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45573..46595)
                     /gene="yceG"
                     /locus_tag="JN749_000239"
     CDS             complement(45573..46595)
                     /gene="yceG"
                     /locus_tag="JN749_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HAY7366779.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46598..47407)
                     /gene="pabC"
                     /locus_tag="JN749_000240"
     CDS             complement(46598..47407)
                     /gene="pabC"
                     /locus_tag="JN749_000240"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HAY7366780.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47527..48768)
                     /gene="fabF"
                     /locus_tag="JN749_000241"
     CDS             complement(47527..48768)
                     /gene="fabF"
                     /locus_tag="JN749_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAY7366781.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48855..49091)
                     /gene="acpP"
                     /locus_tag="JN749_000242"
     CDS             complement(48855..49091)
                     /gene="acpP"
                     /locus_tag="JN749_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7366782.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(49302..50036)
                     /gene="fabG"
                     /locus_tag="JN749_000243"
     CDS             complement(49302..50036)
                     /gene="fabG"
                     /locus_tag="JN749_000243"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HAY7366783.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGSQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(50049..50978)
                     /gene="fabD"
                     /locus_tag="JN749_000244"
     CDS             complement(50049..50978)
                     /gene="fabD"
                     /locus_tag="JN749_000244"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAY7366784.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50994..51947)
                     /locus_tag="JN749_000245"
     CDS             complement(50994..51947)
                     /locus_tag="JN749_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAY7366785.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(52015..53085)
                     /gene="plsX"
                     /locus_tag="JN749_000246"
     CDS             complement(52015..53085)
                     /gene="plsX"
                     /locus_tag="JN749_000246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAY7366786.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(53166..53339)
                     /gene="rpmF"
                     /locus_tag="JN749_000247"
     CDS             complement(53166..53339)
                     /gene="rpmF"
                     /locus_tag="JN749_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAY7366787.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53391..53912)
                     /gene="yceD"
                     /locus_tag="JN749_000248"
     CDS             complement(53391..53912)
                     /gene="yceD"
                     /locus_tag="JN749_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HAY7366788.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            54111..54695
                     /gene="yceF"
                     /locus_tag="JN749_000249"
     CDS             54111..54695
                     /gene="yceF"
                     /locus_tag="JN749_000249"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HAY7366789.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54807..55766)
                     /gene="rluC"
                     /locus_tag="JN749_000250"
     CDS             complement(54807..55766)
                     /gene="rluC"
                     /locus_tag="JN749_000250"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HAY7366790.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            56339..59524
                     /gene="rne"
                     /locus_tag="JN749_000251"
     CDS             56339..59524
                     /gene="rne"
                     /locus_tag="JN749_000251"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HAY7366791.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59721..60674)
                     /gene="flgL"
                     /locus_tag="JN749_000252"
     CDS             complement(59721..60674)
                     /gene="flgL"
                     /locus_tag="JN749_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HAY7366792.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60686..62328)
                     /gene="flgK"
                     /locus_tag="JN749_000253"
                     /pseudo
     CDS             complement(60686..62328)
                     /gene="flgK"
                     /locus_tag="JN749_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62394..63335)
                     /gene="flgJ"
                     /locus_tag="JN749_000254"
     CDS             complement(62394..63335)
                     /gene="flgJ"
                     /locus_tag="JN749_000254"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HAY7366793.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(63335..64432)
                     /locus_tag="JN749_000255"
     CDS             complement(63335..64432)
                     /locus_tag="JN749_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HAY7366794.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64444..65142)
                     /gene="flgH"
                     /locus_tag="JN749_000256"
     CDS             complement(64444..65142)
                     /gene="flgH"
                     /locus_tag="JN749_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HAY7366795.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(65195..65977)
                     /gene="flgG"
                     /locus_tag="JN749_000257"
     CDS             complement(65195..65977)
                     /gene="flgG"
                     /locus_tag="JN749_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HAY7366796.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(66115..66866)
                     /locus_tag="JN749_000258"
                     /pseudo
     CDS             complement(66115..66866)
                     /locus_tag="JN749_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(66886..68805)
                     /gene="flgD"
                     /locus_tag="JN749_000259"
                     /pseudo
     CDS             complement(66886..68805)
                     /gene="flgD"
                     /locus_tag="JN749_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(68817..69220)
                     /gene="flgC"
                     /locus_tag="JN749_000260"
                     /pseudo
     CDS             complement(68817..69220)
                     /gene="flgC"
                     /locus_tag="JN749_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(69224..69397)
                     /locus_tag="JN749_000261"
     CDS             complement(69224..69397)
                     /locus_tag="JN749_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HAY7366797.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69552..70211
                     /gene="flgA"
                     /locus_tag="JN749_000262"
     CDS             69552..70211
                     /gene="flgA"
                     /locus_tag="JN749_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HAY7366798.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            70287..70563
                     /gene="flgM"
                     /locus_tag="JN749_000263"
                     /pseudo
     CDS             70287..70563
                     /gene="flgM"
                     /locus_tag="JN749_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
     gene            70568..70984
                     /gene="flgN"
                     /locus_tag="JN749_000264"
     CDS             70568..70984
                     /gene="flgN"
                     /locus_tag="JN749_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HAY7366799.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(71024..72559)
                     /gene="murJ"
                     /locus_tag="JN749_000265"
     CDS             complement(71024..72559)
                     /gene="murJ"
                     /locus_tag="JN749_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HAY7366800.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSHLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72726..73592)
                     /locus_tag="JN749_000266"
     CDS             complement(72726..73592)
                     /locus_tag="JN749_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7366801.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(73594..74241)
                     /locus_tag="JN749_000267"
     CDS             complement(73594..74241)
                     /locus_tag="JN749_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HAY7366802.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(74252..74836)
                     /gene="rimJ"
                     /locus_tag="JN749_000268"
     CDS             complement(74252..74836)
                     /gene="rimJ"
                     /locus_tag="JN749_000268"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7366803.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            75072..76280
                     /gene="mdtH"
                     /locus_tag="JN749_000269"
     CDS             75072..76280
                     /gene="mdtH"
                     /locus_tag="JN749_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HAY7366804.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEYR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            76344..76991
                     /gene="grxB"
                     /locus_tag="JN749_000270"
     CDS             76344..76991
                     /gene="grxB"
                     /locus_tag="JN749_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HAY7366805.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYHDNMAKQTQINLLSSMAI"
     gene            77125..77685
                     /locus_tag="JN749_000271"
     CDS             77125..77685
                     /locus_tag="JN749_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7366806.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77791..78837
                     /gene="pyrC"
                     /locus_tag="JN749_000272"
     CDS             77791..78837
                     /gene="pyrC"
                     /locus_tag="JN749_000272"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAY7366807.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        18801 a        21202 c        19507 g        19447 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttt gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgcgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgtcgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatag tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgtatgtgct gtatgggtgc tcagtctctg atccctttca gaaattctat
     6601 caatgcatct gtctgttcag gatttactgc cagcatttca ccggatagcg cgcaacgaac
     6661 aaaaccatga tgatcctttt caatcagcgc ccccgtttcc agaaatgcgc ggtaatcagt
     6721 gatattcatc gtctccatgt tgtcagcatg gtatttatca cgctgtttaa gtatctcatc
     6781 aaatttcatc ggcattgcgt tttcctctgt tgtgtctgtt tgtttcagat agtaactatg
     6841 ccggaccgtg acgaaaagcc agtagtgctc cataccgttt caaatgacgc aaaaaaagcc
     6901 ggattgctcc ggcttcgtgg tgtggttctg atattcctac tctgctaatt tgctcagccc
     6961 catgtctgcg taagtgcgat ttactgcatt tctcaggtct gcgtagttct ccgttggcgg
     7021 ctccggtgtg cccttgctgc tctgttttac cagcgggtcg cccaccagcc gcccgtaagc
     7081 tgcaatttgt gctgtcatat tgccacctca gtgaagcgat ccggcattgt tctttttcat
     7141 ctcttccatc attttttcag tgagcattac gcactgaaac cccatgtttt tgatgtctac
     7201 gggtttcatg cctttgtcag tggatatcac aggcagaata tcctgataca catcgcggtc
     7261 agggtaacta agcacacaaa gcgcccatga tgccacgggt gaggatcctt cgtctgtttc
     7321 aacccaccag cctggtgtag ctgcgcttat ttgcagaatc gtgatccgtc cgttgtgctt
     7381 gtaaaatttt tcaccaaatt ttggttcgct catgattcca cctctccggt tttaacgttg
     7441 attgttgtta cctgttccgc ttcggcaatc tcccgctctg tcagcgtggc aaaatttgcc
     7501 gccgtcgtgg tcatgaatgc gcttatcagt tcgggatgtg ctttcgcgta tccttccccc
     7561 gcgtggcggt ctatcgttct gattgccacc tttaaagcgt gctctgtcat gtctaacgcg
     7621 cgatatttcg gttctgttct gtctctgcgt tttttgagtg atttattaaa tttccctgaa
     7681 gtgtgcatat ttatttttac cccctcgttt aaaaagtttt gagttgtgcc tccccttgtc
     7741 taccttagtg gccctcatgc cagtaatggc gctgctttca gcggggtaga gtgcttttat
     7801 ccactatcta ccccgtgtct acctccctgt ctgattcagg taaaatcagg tagagagggt
     7861 agatagtggg tagacagtaa aaaaaggcta cctacctaac ttaatgcact gaattaaatg
     7921 tatttttctt tactcaggta gatagggtag acagcaatta caaaaaatta taaaaacgcg
     7981 tcgcactcgt ctgttgttat tgcgttagtc tgcgttactc ccttaacttt ccgcgtaata
     8041 tattcatgtc cgtaaacttt cgcggctggc ttcatagcct tgccaaagtc atttacgttt
     8101 agcggtttgc tcctgcctgc gtacgccata aacgccagat agacgcggta aaggctgttt
     8161 ctggtcgtgt acttcactga atcgccaccg ccgcccatca tcaggccgcg tgcttcctcc
     8221 agaaaattca ggaactggca aaactcaata accggatccg tctgttgctt tattgccaga
     8281 gcttcatcac cgtcacgctg ttccagtagt aaagcccgtg ctttctcaga gtcggtaaag
     8341 ttcgccagca atcggcggat aatgacaggg atttcagccg caatcttttc cggtagctcc
     8401 ctgtcttttt cggcctcact aacgatattg tcgaaacgga aaatcacgcg acgacgtgcc
     8461 acacctccgg cccgttcggt gaatatcatc gggttattgt tggtcgccag caccaccgcc
     8521 ctgattacag ccgtgaaacg cttttcgtat ttcgggttaa tttccacggg gtcaccgccc
     8581 gtgattttct tgatgcccgt tccttcgcct gtatatttcg gctggtcagc caggacgata
     8641 agacgactcc cgacaacctg cggacgtcca ccagcatcat caagcgatgt catttcagcg
     8701 cttaccgtgt tctgtttccc tgccagaagg ctggctatgt gtgtgaatgt acttttaccg
     8761 ctcccgccgt ctccggtggc ctcaataaac atctgccagt cgtaccggtt cgccataatc
     8821 atgtacagcg cggcacatat acgcatcatc ttgcgctggt cttttccggc tgcgtgctca
     8881 agccatttat gaaagtttag cgcgttatcg cggatgttct cccctggtgc tggtggcgtg
     8941 tactcaatgc cgttgtgcgt ggtgatccag ttctccggcg tgtgcgggga aaattccccc
     9001 gttttcaggt caagcgcacc attggcgaac ggcagcaaat cgccggacgg ctcgcccatt
     9061 ggttcggcaa taacttttaa cgcttccacg gcgttattga ttacgcgctt gctgaaagtg
     9121 gccctgtgct ctgaatagat cgccaccatt tcgcggctaa gttccattgt gctgaccgga
     9181 caccataccc cgccgcgcca tacgtgaacg atttcacttt cctgatgcac acaaacgcca
     9241 tcaaagcgct cggcaagcag ctgcgcgcgc tcactgtctg ccatttgtgc cagttgcgta
     9301 tttttctgct ccggcatggt aagccctgcg gcaatatttt cacgctcagc attcagatag
     9361 cgccgccagt tttcacactt ctggacgtgc attccttcgg ggtaaaaatt tgcgtcctga
     9421 atatctgccg ccgccagctt ctgaccaatc tttttggtct ccaccaaatc cagttctccg
     9481 gcctggtaca gccttacgcg tttttttcca tccggaacaa ttttcagtgc atcaagttcg
     9541 gcaagctgat ttggcccaag ccacacaggc ggcacattat cgccggatgc ggggccatcc
     9601 tgctcctgcc attgtttcgc gtgcgaccag gcatcactac ccgcaaagat aattacctct
     9661 gtgtctttgt gttttattcc gcgtggctgt ttttttacgt tcggtgccag tttcattttt
     9721 tacccctgaa tacgttaagc atctttttta tttcctgaat attggcgcgt gctttctccc
     9781 tgctggttgg tttactgcgg ggcgctgcct gtaccagcgt gaaatcacgc cggaattgat
     9841 aaacaggcat cacgcaatca tattcgtaac cttcacggcg gtaggtgatg cgccgttctg
     9901 ccacgccttt aatcgttacc atgccgccgt actggtcgcg gtaaatatca ccgcgcgtaa
     9961 atttagggtg agtgttgcca ctggcagtta agccagaata tttaagtttc attattttta
    10021 ttctccggtg tgctgttctt tatatctgtc gtgcaataga tctatttctt gcagttccat
    10081 tattacaggc tcaagaagcg ttattaatgc cgtggcaatt cttgattttt gtttgtcgcg
    10141 ttcattgtcg ccaagtgttt caagccatat gcgcaatatt tccagcatgt tttcactgtg
    10201 agaaagtgca agaaatgcgc ggtctattgt ttcgtggtaa atatcacgca tattaatccc
    10261 cgtccgtcgt ttttcttaaa ataacctctg tcacgaaatc agcataatcg gcggcgatat
    10321 tcagtacatc aagcccgttg atttatattc tctcgtggaa agtaagaaaa aagccgctct
    10381 aataagttct ggcattgacg aaagcgcatc agccgcatca tcaggaacgc cggaaaattc
    10441 ctgtttcagg gaattaaaac gatcatcacg cataaccccc cccattttca caatcggcaa
    10501 caagaatatt tttagcgtca ttcagtgacc gcgttgcgat gtatcggata cattccaggg
    10561 cgaactgtgt ccggtctttc tgttcttctc cctgcgcaag ctctgccgtg cattcaatat
    10621 caataagcgc gtgcatcagc gtagtgagtg cggcggctgc tgcgttcggt gtggttttat
    10681 tgtgcatagg tttccccctg acgaatacgg gcggcgaata cagcaacaca accggacggg
    10741 caacggctac gcgcttcgcg ttccgtccaa gcggttacgt ggatgatttg ggattctccg
    10801 gtactcagtg ccagaaaacg ccacacaaag gccgtttgtg tgtgtacaag gtgtggtata
    10861 tgatttacgg caaccataac ggctcctagt ttacgttgtt ggttagaagc cccgttactg
    10921 ctcctacagt gcggggcttt gctttttgtg ttgattgtgt gtaatgtgtc attacacata
    10981 aatacattac atgaggtgta attaacgtgt caacacacaa aaacgaaagg cgtggtaatc
    11041 ctccgttcca gttccgttta gatcctgact tacgggaaaa gatggaaaag gcacaacaac
    11101 aggacggcga tgattctctt gctgcctgga taaagcgaat aatccgcaaa gaattacagc
    11161 aacgcggcat agaacccaaa ggataaaaac tatcagcgcc gtggtgtgag gtactacggc
    11221 gcattgcttt acaggcgcac acaatgacca acgaagaatc aaccgaaaaa acatcacctg
    11281 cacgaaaaag acgacgcaga aagaaagagc atgaatcaga acgattcgcg ccgtgttctt
    11341 ttgcccttga gaagtacctt aaagaatact catcaaagga gagaacttcg cagttatggc
    11401 aacgcactga accagactga ttgcattgcc caccagcctg atagcggcta tcatccccgt
    11461 gcttatgttt gggatcacac acacaaacgg cgcagcgggt tatctgttca gaaaggcggc
    11521 tccatttcgg ggccgctttt tttatgcctg aaaaacccca attttgtggt tttccagttt
    11581 caccagggcg aacgaatccc cgcccacgtt ctggcgtata ttcaatcttc atggttatag
    11641 ctctgtgttc agatgattga tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg
    11701 ccttgcagct atccttccag gccagaacct cggataaaga ccagccaacg gaacgaccgc
    11761 caagtttacg acgtgatggg aattgtccgg ccttttccag gcggtagcgg catgagcggc
    11821 taaggcctgt tagcttttcg cattcttttt cacgtataaa ccgatcagtg cttaacacta
    11881 ttgccccctt tcgtttctta aagagttatt tcgtgttcta ttgcgttggg atgtgtctga
    11941 ttgtgtcagg atgattcaga gttggcaaat gttgaggccg tatggtttac agaaagagga
    12001 ataatcagga taaaatcatt taaattcatg ttaatacaaa ggcataaaat attgtttcat
    12061 gccttttttc tcgctattta aagagtgatt cgctagtgta taaaaaacca gtaagacgtt
    12121 aaaaatcagc tacttataaa tctgtacact ttttcacctc ttgttcgtag ttgttccgca
    12181 ttgttgctca ttgttgctca ttgttgctca ttgttgctca ttgttgcacg ttgtatctgt
    12241 tcgcatatcc agtatgcgca tactgaaaaa acacgaaaaa aattattttc ttctggctac
    12301 tggtagcgtg gttacgtttt catgtgttcc cgccagtatc cctaaccgct ccgtccacat
    12361 atccagcgca ttgcgtttag catccagata acgggaatga ttataaactc gctgcattcc
    12421 tggcatctgg tggcctgtaa gctgctccac gacgtgagga tccacgccta aatcgttcag
    12481 catcgttgta aaggtgcgcc ggatgtcatg tagtgaccag tgagggtgat ttagcctcct
    12541 gtgcgctaat ctgccgtact gcgacacgct tgtttcctgt ttcacttccc ccagcaataa
    12601 gcccgtgtgc ctgttctgct ccaccagctg cgtgacgaac ggcagtattg cttccggtat
    12661 gggccggaat attgccacct tcgttttgct gtgttctttc ggcacggtcc agagcatttc
    12721 ggtaaaatcc cactcgctga tctccgataa cctcagttct accgtccggc atccgaacac
    12781 aatcaggagg cggattaacg cgatgtagta gggggagaat atttttttgt ccagtgcctg
    12841 caataattcg cccagttctt tggtgcttaa gacacgctcg cttatatccg gttttttccc
    12901 aacgtccgcc acactcatat catcaagaac gttgctgatt gcatagcgcc gcctccggca
    12961 gaacttaagc gcctgtttgc acgtctgtag caagaatccg gcagtaacag gcgttcgctt
    13021 tgccacctgg tcaaaacagg ccagccagtg ccgtagctcg catttatcca gcggcatagc
    13081 accaatgtgc tgtattacgt gattattaag gcgctttttc agggcggcat aatccacgcg
    13141 gttttccttt gcgtacgact caagccagta ggtgagcgca tcgccaaccg ttaccggctt
    13201 taacgcttcc tgtacggtgt aattaagctc atgacgtgga tttttcccct ctgccagcca
    13261 tgcgcgacac tgggcggctt tttccctggc tgatttcagg ctcagatcag gataatttcc
    13321 cagcttaatg cgttccggtg gtgcccccct tcccgttccg gccctgtaag tgaaatacca
    13381 ggttaaaagg ccactggttg aatgcctgac gctcaggttt ccaccgtcat taagaaaggc
    13441 tgttttttgg gcgggtgtgc cgttgatttt cctcagctgt gtatcgctca gtttgttaag
    13501 tgctctgctc ataattttga ttccggtcat cacaattaac tacaccatta actacaccga
    13561 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
    13621 tataatcaaa aacataatga acaatctgaa acattatgaa acggcaaaaa acactaattg
    13681 ataaaatatg tccatacttc acgcattacg ttaagcatcc gttataatcg gttgcagata
    13741 ccagcctgtg gatgcttaac atggaatacc aactcactct taactggccc gattttcttg
    13801 aacgtcactg gcagaaacgc ccggtggtgt taaaacgcgg ctttaataat tttattgacc
    13861 cgatctctcc agacgagttg gcgggtctgg cgatggaaag cgaagttgac agtcgactgg
    13921 tcagtcacca ggatggcaaa tggcaggtca gccacggccc gttcgaaagc tacgatcatc
    13981 tcggtgaaac caactggtca ttactggtac aggcagtaaa ccactggcat gagccgaccg
    14041 ccgcgctgat gcgaccgttc cgtgaactac cggactggcg tattgatgat ctgatgattt
    14101 ctttttctgt acctggcggc ggcgtcggcc cgcatctcga tcagtacgac gtgtttatca
    14161 ttcagggtac cggacgtcgt cgctggcgag tgggcgaaaa gctgcaaatg aaacagcact
    14221 gcccacatcc agatctgtta caggtcgatc cgttcgaagc catcatcgat gaagagctgg
    14281 agcctggtga tattctttat attccgccag gattcccgca tgaaggctac gcgctggaaa
    14341 atgcgatgaa ctattccgtg ggttttcgcg cgccaaatac gcgggaatta attagcggat
    14401 ttgccgatta tgtgctgcaa cgtgaactgg gcggcaacta ctacagcgat ccggatgttc
    14461 cacctcgcgc tcatcctgcg gatgttctgc cgcaagagat ggataaactg cgtgagatga
    14521 tgctcgaatt gatcaaccag ccggaacact ttaagcaatg gtttggcgag tttatatccc
    14581 agtcacgtca tgaactggat atcgcgccgc cggaaccgcc ttatcagcca gatgaaatct
    14641 acgatgcact gaaacaaggt gatgtgctgg tgcgcctggg tggtctgcgc gtattgcgca
    14701 ttggcgacga cgtgtatgcc aatggtgaga agatcgattc cccgcaccgt ccggcactgg
    14761 atgcactcgc cagcaacatt gcgctgactg cggagaattt tggcgatgcg ctggaagatc
    14821 cgtcattcct cgcgatgctc gcggcgctgg tcaatagcgg gtattggttc ttcgaagggt
    14881 aagtttggat ttaagccgga tgcagcatca catgccgcat cccttttcgc ttacttccgt
    14941 tgcgccgtta actcggcaat acggacgatc acctgcaccg ctttttccat accttccaga
    15001 atcacaaact catgcttacc atgatagttg taaccgccag tgaacaggtt cgggcaaggt
    15061 aatcccataa acgacaactg cgcgccgttg gtaccgccgc ggatcggttt cagttccggt
    15121 tcaatatcgc agtcacgcat cgcctgctgg gcgatatcga gaatatgcgg atgctcaacc
    15181 actttctcgc gcatattgta gtaactgtct tcaatcacca attcaatgta gcaatcagga
    15241 tgtaaccctt tgcccacttt tttggcgatc tccatcattt tacgtttacg cgcttcaaac
    15301 tgtttacggt cgaaatcacg gatgatgtag tgcatatcgg cccgttcaac ggtgcctttc
    15361 atgctcgcca ggtgatagaa accttcatag ccttctgtca tttccgggct ttcatccgcc
    15421 ggaacttccg catgaatacg tgccgccagc gacagcgcat ttaccatcac tcctttcgcc
    15481 gtgcccggat gaacattgtt accgacaatt ttgatattga ccgatgcggc gttgaagttt
    15541 tcaaactcca gttcgcctac gccaccaccg tcaacagtgt aagcccagcg ggcatcgaag
    15601 gcatcaacat caaaatgttt cgcccctttg cccacttctt catccggggt aaaggcgacg
    15661 cgaatatcac catgcggaat gtttttctgt tgcaataccg ccagcgcggt catgatttct
    15721 gcaatacctg ctttgtcatc ggcacctaac aaggttttac catcggtggt aatcaacgtc
    15781 tgacccagta gctgatgcag caccgggaac ataaccggtg ataaaacttc atcgccgata
    15841 cccagcgcaa tatcgccacc gcgatagttt tcaacaattt gcggattaac atttttgccg
    15901 ctgcaatccg gtgaggtatc cacatgagaa ataaagccaa tcgccgggat atcgccaggg
    15961 acgttagccg gtaacgtcgc catcaaagtg cccttctcac ttaaggtcac attgataagc
    16021 cccatttctt cgagctgctc tttcagcaga tgcaataact tccattggcc ttccgtgctg
    16081 ggaacctgtc tcacccctgc ttttgattgg gtatccagag acacgtagtt caaaaatcgc
    16141 tcaagtagtt tatccatgta gtcaccctca ctttttgtga caacattatt aagaagcaag
    16201 aaaagacaaa tattgcgtca ggtcactttt acccctgcaa gcgggaatat ttatcagcat
    16261 taccttaatg aatataaagc taagccagta attcacaaca aagattggcg attcaagcgg
    16321 tttgccgtag aattaccgcc ctcattaaga gtcaccaagg tggttaacca caaaccccgc
    16381 atcggtaagc catccgttgc gtttacatgg gacagagtaa aaaattgaat aaacaaccga
    16441 gttcgctttc accgctggtg caattggcgg gaattcgcaa atgctttgat ggtaaagagg
    16501 tcattcccca gctggatctg actatcaaca atggcgagtt cctcacgctg cttggccctt
    16561 ctggctgcgg taaaacaacc gttcttcgcc tgattgcagg tctggaaact gttgattccg
    16621 gacgcatcat gctggataac gaggacatca cccacgttcc ggcggaaaac cgctatgtga
    16681 acactgtttt ccaaagctat gcacttttcc cccacatgac cgtgttcgaa aatgtggcct
    16741 ttgggttgcg catgcaaaaa acccccgctg ctgaaattac gccccgcgtg atggaagccc
    16801 tgcggatggt gcagctggaa accttcgctc aacgtaaacc gcatcagctc tctggtggtc
    16861 aacagcaacg cgtcgccatt gctcgcgcgg tggttaacaa gcctcgtctg ttgttgctgg
    16921 atgagtcgct ctcagcgctg gattacaaac tgcgtaagca aatgcagaac gagctgaaag
    16981 cgttacagcg taagcttggc attacattcg ttttcgtgac tcacgaccag gaagaagcac
    17041 tcaccatgtc ggacaggatt gtggtgatgc gcgatggtcg cattgagcaa gacggcacgc
    17101 cgcgtgaaat ctacgaagag ccgaaaaacc tgtttgttgc cgggttcatt ggcgaaatta
    17161 atatgtttaa cgccacggtg atcgagcgac tcgacgagca gcgcgtacgc gccaacgttg
    17221 aaggccgcga atgtaatatc tacgttaact tcgccgttga accggggcaa aaactgcatg
    17281 ttctgctgcg cccggaagac ttacgtgttg aagagattaa cgacgacaac cacgctgaag
    17341 ggctgattgg ttacgttcgt gagcgtaact acaaaggcat gacgctggag tcggttgttg
    17401 aactggaaaa tggcaagatg gtgatggtca gcgaattctt caatgaagac gatcctgact
    17461 ttgaccactc tctcgaccaa aaaatggcca ttaattgggt agaaagctgg gaggtcgtac
    17521 tggctgatga agaacacaag taagttccag aatgtagtga ttgtcactat tgtcggttgg
    17581 cttgtgttgt ttgtctttct gcccaacctg atgatcattg gcaccagctt tttgacccgc
    17641 gacgacgcca gtttcgtcaa aatggtcttt acgctggata actacacgcg tctgctcgat
    17701 ccgctctatt ttgaagtgct attgcactcg ctgaatatgg cgctgatcgc caccctcgcc
    17761 tgcctggtgc tgggctatcc atttgcctgg tttctggcga agctgccaca caaggtgcgt
    17821 ccgctgctgc tgtttctgct gattgttccg ttctggacca actcattgat tcgtatctac
    17881 gggctgaaaa ttttcctcag caccaaaggc tatctcaacg agtttttgct ctggctgggc
    17941 gttatcgaca caccaatccg tatcatgttc acgcccagtg cggtgattat cggtctggtt
    18001 tacattctgc tgccgtttat ggtgatgccg ctgtactcca gtatcgaaaa actggataag
    18061 ccgttgcttg aggcggcgcg cgatctcggt gccagcaagt tacagacttt tatccgcatc
    18121 attattccac tgacgatgcc ggggattatt gccggatgtc tgctggtgat gctgccagcg
    18181 atgggcctgt tctatgtgtc cgacctgatg ggcggtgcga aaaacctgct gatcggtaac
    18241 gtcatcaagg tacagttcct caatattcgt gactggccgt ttggtgcagc caccagcatt
    18301 acgctgacta tcgtaatggg cctgatgttg ctggtttact ggcgcgcttc tcgtttgctg
    18361 aataagaagg tggaactcga atgatcggtc gactgcttcg cggcggtttt atgaccgcta
    18421 tctacgcgta cctgtatatc ccaatcatta ttttgattgt gaactccttt aacagctcgc
    18481 gctttggcat caactggcag ggttttacca ccaaatggta tagcctgctg atgaacaacg
    18541 atagcctgtt acaggcagcg cagcattcac taacaatggc ggtgttttcg gcgacgtttg
    18601 ctacgcttat cggttcactg acggcagttg cgctgtaccg ttatcgcttt cgcggtaagc
    18661 cgttcgttag cggaatgctg tttgtggtaa tgatgtcacc agatatcgtg atggcaattt
    18721 cgctgctggt gctgtttatg ctgctgggta ttcagcttgg cttctggtcg ctgctgttct
    18781 cgcatatcac cttctgcctg ccatttgtgg tggtgacggt gtattcgcgc ctgaaaggtt
    18841 ttgacgtgcg gatgctggaa gcggcgaaag atctcggtgc cagcgaattt accattctgc
    18901 gaaaaatcat tctgccactg gcaatgccag cggtggcggc gggctgggtg ttaagcttta
    18961 ccctgtcgat ggacgatgtg gtagtttctt cattcgtcac cggaccaagt tatgaaattc
    19021 tgccgttaaa aatttattcg atggtcaaag tcggcgtatc gccggaagtt aacgcgctgg
    19081 caaccatatt actggtgctg tcgctggtga tggttattgc cagccagctt attgctcgtg
    19141 ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa tggtcacgcc acctgctcgc
    19201 ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc gatgacaaca acacgctgta
    19261 tttctacaac tggaccgagt acgtgccgcc aggactgctt gaacagttca ccaaagaaac
    19321 cggtattaag gttatctatt cgacttacga gtcgaacgaa accatgtacg ccaagctgaa
    19381 aacgtacaaa gacggtgcct atgacctggt ggttccttca acctattacg tcgataaaat
    19441 gcgtaaagag gggatgatcc agaagatcga caagtcgaag ttaacgaact tcagcaatct
    19501 cgatccagac atgctcaaca agccttttga cccgaataac gactactcca ttccgtatat
    19561 ctggggtgcg acggcgattg gtgttaacgg tgatgcagtg gatccgaaat ctgtcaccag
    19621 ctgggccgat ctgtggaagc cagagtacaa aggcagcctg ctgttgaccg acgatgcccg
    19681 tgaagtgttc cagatggcgc tgcgtaagct gggctactcc ggtaacacca ccgatccgaa
    19741 agagattgaa gctgcatata acgagctgaa aaaactgatg ccaaacgtcg cagcgtttaa
    19801 ctccgataac ccggctaacc cgtacatgga aggcgaagtt aacctcggca tgatctggaa
    19861 cggttctgct ttcgttgcac gccaggcggg tacgcctatt gacgtggtgt ggccgaaaga
    19921 aggcggcata ttctggatgg acagcctggc gatcccggca aatgccaaaa acaaagaagg
    19981 cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg gcaaaacagg ttgctgaaac
    20041 tatcggttat ccaacgccaa accttgcggc gcgtaagctg ttaagtccag aagtggcaaa
    20101 cgataagacg ctctacccgg atgctgaaac cattaaaaat ggcgaatggc agaatgacgt
    20161 tggcgcagcc agcagcattt atgaagagta ttatcagaag ctgaaagcag gacgttaatt
    20221 caacaagcgg acggtggtat taccgcccgt tttttagtta gcaaggatgt ggctaatgag
    20281 tcaggacagt aaagttttat cccgctcttt cgtcggtatt gtggtccgct tgtaggcctg
    20341 ataagcgtag cgcatcaggc aatgcttcct gctttataat ccctccagca acttttcaac
    20401 aaattctggc accacctggc ttgccgggcc gtaatatttc tcggcaaatt cattaccaac
    20461 ctgactcggt tcaagattca gttcaacggt gtgagcacca tgtagtttgg cttcgtgtac
    20521 aaacccagca gccggataga catgcccgga agtaccaatg gcaataaaga catccgccat
    20581 cgacaacgcc atataaattt catccatgcc gagtggcatt tcgccaaacc aaacgacgtg
    20641 cggacgcaag ggtgccggga agtggcaaca atggcattta tcttctggcg taacgtctcc
    20701 ggtccagtcg agaacctgac cactttgcga acaacgaact ttcagcaatt ccccatgcat
    20761 atgaatgaca ttggtattac ctgcgcgttc atgcaggttg tctatattct gcgttaccag
    20821 caaaaagcga tcgccgagag cgtcttgcag tttagccagc gcaagatgcg cggcgttagg
    20881 ctgaatttct ggctgctgca gctgtcgacg acgggcgtta taaaacgctt gcaccagttc
    20941 aggatcgcga tcgaaacctt ccggcgttgc cacatcttca acccgatgtt cttcccacag
    21001 gccatctgcg gcgcgaaagg tacgaatacc tgattccgca gaaattcctg cccctgtcag
    21061 tacgagtact cttggttttt ccattgcttc cggcaccact ttatctctga aaaaaatacg
    21121 ctgacgcaaa cgctcgcgca ggcgacgttt atttttacga aaacgactta accgatgacc
    21181 ccgacgcgac agcatagcaa cctctgttgt ttaatcggtt agatgtagga aggccgcacc
    21241 acgcattcct cccgcatcac cgtggcgcgc gcgttcaatg cgcggaacac gagctacagg
    21301 taagagatga caaggcaacc tgtccgccag ttgcgttgtg attgccggga aattcgataa
    21361 gccaccacca atgacgacca ggtcagggtc aacaatggtc aggatatttc ccagagaaac
    21421 cgctaataaa tccagataac gctcaacgtg cgcccttgcc tgctcatcgc cttgatcata
    21481 aagcgcaata atttcgggag cctgcaacgg ttgatgataa tagtgttgat acagccacgc
    21541 aaaaccgcga ccagacagat aattttcaat gcagccatgc tgaccacagc cgcagcggcg
    21601 taacgggaaa tccaacccca tcatggttaa cgcatcaacc ggcagacgca tatggccaaa
    21661 ctcgccggta atgtagcttt taccagtaat cggtttgccg ttgaaaacca gcccgccgcc
    21721 aacgccggtg ccgagaatta accccatcac cagtggatat tgcgtaaatt catcctccca
    21781 ggcttctgaa agggcaaaac agttggcatc gttatcaagg cgtacatcgc gatcaagacg
    21841 tgcgctcagg tcggcacgca gcggtttgcc gctggcagcg gggacattag cggcatacag
    21901 cttaccatct tctgtttccg gcattcccgg aataccgatg ccgacagaac ctttacagcc
    21961 aaaacgttgg tcagcttcag ctaccagctc acacactgca tctaaaaatg cgtcatagct
    22021 gtcacgcggt gtcggcaccc gcttttccca ctgcaactgc cgaccgctat caaacacgcc
    22081 aagcgcaatt tttgttccac caatatcaaa cccgtaatac attgccgctc cttgttttaa
    22141 tgtactgcct ttactggccg ctaaggactc gcgcagggtc aatattactg gcgcgccgcg
    22201 ccggatacca acttgccaaa agactcagca acaatgctgt gaccagtacg tagaagacgt
    22261 ccagccagtg caattccgat ggcaggaagt caataaaata gatatcgctg gagaggaact
    22321 gatgaccgat cagcttttca atccactcaa taatcggggt aagttgcagt gaaacaacta
    22381 cgccgataac caccccacac aggctgccga acagccctgc cagcaatcca taccagacaa
    22441 agatggcgcg aattaaacca tctttcgccc ccagcgttct taatactgcg atatcgccac
    22501 tcttgtcttt caccgccatc actaaggtgg agacgatgtt gaaacaggcc acgccaatca
    22561 ccagtaccat cgccagatac ataatggcgc ggatcatctg gatatcgcga tacatatagc
    22621 cgtaagtacc aatccaactt ttaatataaa catagctgtt ggtcacttca cccgcatcgc
    22681 gtaccagctt attggcgttg aaaacatccg tcattttaag ggcaatacct gacacgctgg
    22741 aacccatatc aagatattgt tgggcatccg ccagcgggat catggcaaaa ctgtgatcga
    22801 gttgaccact caactgcaaa ataccggcaa tgtgcaaacg cacacgtttt ggctgcatca
    22861 gtttatgctc gggattcgag ttggggatca tgatcgacac ccaatcgccc tgcttcactt
    22921 tcagcgcatc cgccacgcct ttgccgatga taatttgctg ttcgcccgct ttaaaattgc
    22981 gccacgcatc cccctgaaca aacgagggta atgcgctcag acgctgttcc tgttgtgggt
    23041 taacgccctt cacctggatt gcgcgcagat tcgctccact ttccaccaga ccggtgaaat
    23101 tgatatacgg cgcagcggcg gcaatacccg gcactttctg cacgttatcc agtgcttcct
    23161 gccagttagt ccatggctga tttaccgctt cgatttcgcc atgcggcacc accgccagaa
    23221 tgcggttatt cagttcgcgt tcaaagccgt tcatcgcgct taagccgacg atcaataccg
    23281 ccacgccgag ggcaatgcca atggtagaaa tgacagagat tagcgacacc atgccgccgc
    23341 gtcgccgtcc gcgactaaaa cgcaggccaa tcaataacga taaaggcatc gccattactc
    23401 cgcccccatc aggctcagtt ccgccgtcag acgtccatca cgcatttcca gttggcggct
    23461 catacgtttc gccagttgca ggtcgtgagt gaccaccagg aaagcggtgc cctgcaagcg
    23521 attcaactct ccaagcaact ggaagatgct gtcggcgtta cgcgcatcga ggttaccggt
    23581 aggttcatcc gccagtacca gacgcggatt attgaccagc gcacgggcaa tcgctacacg
    23641 ctggcgttcg ccgccagaaa gttcagatgg gcggtgattc gcacgatgct ccagccccac
    23701 cgcttttaac atctccagtg cacggctgtt gatttcagcg ggctttttct tgccaatcag
    23761 cagtggcata gccacgtttt ccagggcagt aaaatccggc agcaggtggt gaaactgata
    23821 aataaagccc agcttctggt tgcgcagttc tgctttcgcc gctgaagaca gtttgctcat
    23881 cggctgaccg ttaaagatca catcgccgga ggttggcgta tccagcccgc ctaacaggtg
    23941 cagcaaggta cttttaccgg aaccagagct accgacaatt gccatcattt caccttcgcc
    24001 cacgctgaaa ctgacattgt gcagcacatc ggtttgcaca ctgccttcct gatagcgttt
    24061 gcacaggttg tcgcattgca acaggatctt attcataacg taaagcctca gcgggttgtg
    24121 tggcggcagc gcgccatgaa gggtaaagcg tagacagcag cgcgatagcc atcgccacca
    24181 gcgcaataac aatgacctgt aaaggttcga tagccaccgg cagcgccgcg ccatcaagca
    24241 ggacgccgat tatcggcatc agattattta actggctggc aagcagtgcg ccaagcgccg
    24301 ctccgaggat tgcaccgata atcccggcgc tggccccttg caccataaag accatcatga
    24361 tttgtcgcgg agttaagcct tgcgtttgca ggatcgcgac ttcgccctgc ttctccatca
    24421 ccatcagccc cagcgaggta ataatgttaa acgccgcaac agcgacaatc aggctcagca
    24481 gtaagcccat catatttttt tccatgcgta cggcctggaa cagctcgcct ttacggtcac
    24541 gccagtcctg ccatttgctg ccttcaggca gtttttgctg acttaaagag tcaactttca
    24601 gcggctcatc cagccacaaa cgccagccgg taatattggc tgccggataa cgcatcagac
    24661 gcgaggcatc ctcaatattc accagcattt catagccatc gacttcactg ttggcggcga
    24721 aagtgccaat cacattgaac aggcgctgac ttggaatacg ccccatcggc gtgaactggc
    24781 tggcagatgg caccatcacg cggatttgat caccgcgatt gacgcccaac tgtgaggcaa
    24841 gttgttcgcc gaggatgaca ttatatttcc ccggctcgag gtcagtttgt ttcacattga
    24901 ccagatacgg cgttagtgga tctttttgcg ccgggtcgat accgagcatc accccgaccg
    24961 ccacgctgcg cgcgctttgc agtaccacat caccggtagt aataggtgcg acgcgattaa
    25021 cgccgtccag tttgactgcc gtttccggga gttgctgcgg gttaagagag ccatgctcag
    25081 aagagagaat tgcctgtggc atcaggccaa ggatgttgtt ttgcagctcg cgctcaaagc
    25141 cgttcatcac tgacaatact gtgaccagcg ccatcacccc gagggtaatg ccgatggtag
    25201 aaagccagga gacgaaacga ccgaagcgat ccgctgcacg cccacgcatg taacgcaagc
    25261 caataaatag agcgacaggt tggtacatga aatccgtctg gttgctgtag caaagacacg
    25321 agtatataag cgaaagcaag atgggtaaac gtctgaactg taagtgatcg tcagggctaa
    25381 tctgaaaagc ccccgtaaat caatcggcta aattgccatg ttttatcatc aatcttgccg
    25441 cctgatctta atctgaaaaa tcatccaata cagacagata cccattacac ctcgggtcat
    25501 aaccagcagg atcaaccgca tgtaaattaa aaaagagcgt gttgttctga tgaaacaaaa
    25561 agagctatgg attaaccaga tcaaagggtt atgtatttgt ctggtggtga tttatcactc
    25621 ggtcattacc ttttatccgc atatgaccac ttttcagcat ccgttatcgg aagtcctgag
    25681 caaatgctgg atctatttca atctttacct tgcccccttt cgtatgccgg tttttttctt
    25741 tatatccggc tatttgattc gccgctatat cgacagcgta ccgtggggaa attgtctcga
    25801 taaacggatc tggaacatct tctgggtgct ggcgctttgg ggcgtggtgc agtggctggc
    25861 acctgagcgc gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt
    25921 cctgcacggg atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta
    25981 tttcgtggta tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact
    26041 gctgagtgtg gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatacg
    26101 caatttgcct tattacagcc ttggcgcatg gtttggcgca acaataatga cctgtgttaa
    26161 agaggtgccg ttgcgccgcc atctgctgat ggcttctttg cttaccgttc tggcggtcgg
    26221 tgcctggttg tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa
    26281 gctgttttat cagtacgagc aacgattcgg tatgcgctcc accagcctgc tgaatgtgat
    26341 tggctccaac accattgcta tctacaccac ccatcgcatt ctggttgaaa tattcagctt
    26401 aactctgctt gcgcaaatga acgcagcacg ctggtcgccg caagtcgaac tgacactcct
    26461 gctggtttat ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa
    26521 actttcacag cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt
    26581 cagttactct cgttaaacca tcaccccagc cggggcgatt caatttgcta aaccatgtcg
    26641 attacggata atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc
    26701 ctaacgagaa tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag
    26761 cgggtgagca gcgtctgctg ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg
    26821 aaattgccga acgtcacgcc ggtccggtgg tactcattgc accagatatg caaaatgctc
    26881 tgcgtttgca tgatgaaatc agccagttca ccgatcagat ggtgatgaat ctggcggact
    26941 gggaaactct tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt
    27001 ccacccttta ccagctaccg acgatgcagc gtggcgtact gattgttccg gtgaatacgc
    27061 ttatgcagcg cgtttgccca cacagttttc tccacggtca tgcgctggtg atgaaaaaag
    27121 gtcagcgcct gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgctatg
    27181 ttgaccaggt gatggagcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc
    27241 cgatggggag tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagcctgc
    27301 gggtgtttga cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc
    27361 ccgcacacga atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg
    27421 ataccttcga agtgaagcgc gatccggaac atatttacca gcaagtgagt aaaggcacat
    27481 tacctgccgg gatcgagtac tggcagccat tgttcttcag cgaaccactg ccgccgctgt
    27541 tcagttattt ccctgccaat accttgctcg tgaatactgg cgatctggaa accagtgccg
    27601 aacgtttcca ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc
    27661 cgctgttgcc accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa
    27721 actggccccg agtgcagcta aaaactgaac atttaccgac aaaagccgcg aatgccaatt
    27781 taggtttcca gaaactgcca gacctggccg ttcaggcgca acaaaaagcg ccgctggatg
    27841 cgctgcgtaa gttcctcgag actttcgacg gtccggtggt gttctcggta gaaagtgaag
    27901 gtcgccgtga agcgctgggt gaactgctcg cacgaattaa aattgctccg caacgcatta
    27961 tgcgtcttga tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg
    28021 gttttgtcga taaggtgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac
    28081 gtgttgcccg ccgtcgtcag gattctcgcc gcaccatcaa ccccgataca ctgatccgta
    28141 accttgcgga gctgcatatt ggtcagccgg tggtccatct ggagcacggc gtcggtcgct
    28201 atgccggaat gaccacgctg gaagctggcg gcattactgg cgagtacctg atgctcacct
    28261 atgccaacga cgccaaactg tatgttccgg tgtcgtcact gcatctgatt agccgttacg
    28321 caggtggcgc ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg
    28381 cgcggcagaa agcggcggaa aaagtgcgtg atgtggcggc ggaattgctg gatatctacg
    28441 cgcaacgcgc cgccaaagag ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt
    28501 tctgcgacag cttcccgttt gaaaccacgc cggatcaggc gcaggccatt aatgcggtac
    28561 ttaacgacat gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg
    28621 gtaaaacaga agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtag
    28681 cggtgttggt gcctaccacc cttcttgcgc agcagcatta cgacaacttc cgcgaccgtt
    28741 tcgccaactg gccggtacgt atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga
    28801 cgcaaatcct tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac
    28861 tgctgcaaag tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc
    28921 gcttcggggt gcgtcataaa gagcgcatta aagcgatgcg cgcgaacgtg gatattctga
    28981 cgcttactgc aacgccgatc ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc
    29041 tgtcgattat cgccacgccg cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt
    29101 atgacagcct ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt
    29161 attatctcta caatgatgtg gaaaacattc agaaagccgc cgaacggctg gcagaactgg
    29221 tgcctgaagc acggattgcc atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg
    29281 tgatgaatga tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa
    29341 ccgggatcga catcccgaca gccaacacca ttatcattga acgtgcggat cacttcggtc
    29401 tggcgcagct gcaccagtta cgcggtcgcg tcggacgttc acatcatcag gcatatgcat
    29461 ggctgctgac gccgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga
    29521 ttgcctcgct ggaagatctc ggggcaggtt ttgcgctggc aacgcacgat ctggagattc
    29581 gcggcgcggg tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct
    29641 cgctgtatat ggagttgctg gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt
    29701 cgctggaaga tctcaccagc cagcaaacag aagtcgagct gcggatgccg tcgctattgc
    29761 cagatgattt catccctgac gtgaacacgc gtctgtcgtt ctacaaacgt attgccagcg
    29821 ccaaaacgga aaacgaactg gaagagatca aagtcgagct tatcgatcgc ttcggcctgc
    29881 tgccagatcc ggcgcgtacc ctgctggata ttgcccgtct gcgccagcaa gcgcagaaac
    29941 tggggatcag gaagctggaa ggtaatgaga aaggcgggat gatcgaattt gccgagaaga
    30001 atcacgttaa tccggcctgg ttgattggtt tgctgcaaaa acagccgcag cattaccgcc
    30061 ttgatggtcc gacgcgcctg aaatttattc aggatttgag tgagcggaaa acgcgtatcg
    30121 aatgggtacg ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tgtaaatcgg
    30181 gggcgtttct gcgcccccat ctgcaacatt tacaaattct ttgcactttc ctgcactatc
    30241 cgacactgtc accatccata attcaggctt atctgtttat tacaataacc ttatatttat
    30301 tatggatttt tggtgatgat caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc
    30361 gccgctgccg tggcgctggc gctaccggca aaagccaaca cctggccgct gccgccagcg
    30421 agcagtcgtc tggttggcga aaacaaattt catgtggtgg aaaatgacgg tggttctctg
    30481 gaagccatcg ccaaaaaata taacgtcggc tttctcgctc tgttacaggc taaccccggc
    30541 gttgatcctt acgtaccgcg cgcgggcagc gtgttaacga tcccgttgcc aaccctactt
    30601 ccagacgcgc cgcgcgaagg cattgtgatc aacattgcgg agctgcgtct ctattactac
    30661 ccgccgggta aaaattcggt aaccgtgtat ccaataggta ttggtcagtt aggtggtgac
    30721 acgctgacac cgacaatggt gaccaccgtt tcagacaaac gtgcaaaccc aacctggacg
    30781 ccaacggcaa acatccgcgc ccgttataaa gcacagggaa ttgagttgcc tgcggtagtg
    30841 ccggctggac cggataaccc aatgggccat catgcgattc gtctggcggc ctatggcggc
    30901 gtttatttgc ttcacggtac gaacgccgat ttcggcattg gcatgcgggt aagttctggc
    30961 tgtattcgtc tgcgggatga cgatatcaaa acactcttta gctaggtcac cccaggcacc
    31021 aaagtgaata tcatcaacac tccgataaaa gtctctgccg aaccaaacgg tgcgcgtctg
    31081 gttgaagtac atcagccgct gtcagagaag attgatgacg atccgcagct gctgccaatt
    31141 acgctgaata gcgcaatgca atcatttaaa gatgcagcac aaactgacgc tgaagtgatg
    31201 caacatgtga tggatgtccg ttccgggatg ccggtggatg tccgccgtca tcaagtgagc
    31261 ccacaaactc tgtaaatcct ggataaaaaa aatccccgcg acacggcatg tcgcggggaa
    31321 aaaatcagca gtagcaggca ttaatgaggg ttaatgctta tttataaatt actgctgttc
    31381 catggagggt attcggaccg gttacagaag taatacggaa agattttgcg cccatctcat
    31441 ccgctttttg cgccagctgc tcttccagcg atcccagatt tgtccccgcg ttagcactga
    31501 tagtaccgac tttttgttgg ccttctggcg ttgactgaac ttcgacagcc gcaaagttgg
    31561 caaatgacat ggagcttaaa atcgccgcag cgatgagggt ttttacgttt ttcataatag
    31621 tggccttatg cagatgaatg acgtcgcaag aaattactta acgatcgata actaaatgat
    31681 agatgtgatc tggatcacat acaagatatt ttttataaca atcattaatt aatttaaaaa
    31741 tctttaaatt tcagtcaaat agaaagcaat tatttttatg ggatctggac tggtgaacgg
    31801 aggtgattat ttttataata acctttcatt cacagtcacc aggtacaacg gcatcatggc
    31861 aactgactca acacaatgtg taaaaaaaag ccgtggccgc ccaaaagtgt tcgacaggga
    31921 tgccgcgctt gataaggcca tgaaattgtt ctggcaacac ggttatgaag cgacttctct
    31981 tgcggacctc gtcgaagcga ccggagccaa agcgcccacg ctatacgcgg aatttaccaa
    32041 caaagagggg ttatttcgtg ccgttctcga ccgctatatc gatcgttttg ccgctaagca
    32101 tgaagcacag ctgttttgtg aagagaaaag cgtggagtct gcgctggctg actattttgc
    32161 tgccatcgcc aactgcttta ccagcaaaga caccccggca ggctgcttca tgatcaacaa
    32221 ctgcaccacc ctctccccag attcaggaga tatcgccaat acgttgaaat cacgccatgc
    32281 gatgcaagag cgcactttgc agcagttttt atgtcaacga caagcgcgcg gggaaatccc
    32341 gacccactgt gacgtgacac atctggcaga attccttaat tgtattattc aggggatgtc
    32401 gatcagcgca cgcgaaggtg catcgctgga aaaactgatg cagattgccg gaacgacttt
    32461 gcgtttatgg cccgaactgg tgaaataagt cgcaaaaaaa gcccctcagc ctgagcgaat
    32521 gaggggccaa attgcagagt acagccttgt tatactttgt tattcaaaat cagttgcccg
    32581 ttactatcta gcgggatctg cgtacccgga tcgcggtcca tgcggatttt gccctgctga
    32641 tcgccaatct tataggtcac atcataaccg agcatttttt ctgacttgtc atacaccgtt
    32701 ttacaacgct gttgcgtagt cgtgtaagta tcgctttcct ggagagagcc ctggatctgg
    32761 ttaccggcat atccaccacc cagcgccccc acaacagtgg cgacatcttt accgcgacca
    32821 ccaccaaact gatgccctat cacgccacca gcaacagcgc cgagcaccga cccggtaatg
    32881 cgattttcat cctgcaccgg tcgacgatgg gtcactgtga cgttgcgaca ctcctgacgc
    32941 ggtgttttaa ccgtttcctt gattggggtt gcagaaacaa cctgagcgta ttgcgggccc
    33001 cgttcaaaca cgttcagact ggccactgcc gctacgccca acgcagctgc gacaccaatc
    33061 ccgatacccg ccaacattga tttattcacg ggaacacctc cttctttgca gttatgccaa
    33121 tcatgcaagc gaaggagaaa agcagctatc agacagaaga tgaaaagcgc gggaatgact
    33181 ggaaatactt aaggattcgg aggagtctga aggaatgcct gatgcgacgc ttaacgcgtc
    33241 ttatcaggcc taccgctcgt accatttgtc cagggcggat aaggcgttta tgccacatca
    33301 gccagtgtac gtcgattaat gcaacttcaa acgcggacgg ataacgcggt taatactccc
    33361 caccagcatc attaatccgg ttttaaagta accatgcagc gcaatctgat gcattcggta
    33421 tagcgagata tatacaaagc gcgcaattcg tccttcaatc atcattgagc cgcgcgtcag
    33481 gttacccatc aggctaccaa cggtggagaa gttcgacagc gataccagcg aaccatgatc
    33541 tttatactga taatttttca gcggcttacc gttcatctgc gccagaatgt tgttcattgc
    33601 gcaagtcgcc atctggtgtg cagcctgagc acgcggcgga acaaagcccc cttccggacg
    33661 cgggcatgac gcgcagtcgc caatagcgta aatgtctgga tcgcgagtgg tttgcagcgt
    33721 cggttccacc accagctggt tgatacggtt agtttcaaga ccaccgatat ctttcaggaa
    33781 gtctggcgct ttgatcccgg ctgcccatac catcagatca gcctcaatat attcgccatc
    33841 tttagtgtgc aggccgcctt catcagcact ggtgaccatg gtttgcgtca gcacgcgaac
    33901 gccaagtttc gttagctcgt tgtgggccgc agcagagata cgtggcggta atgcaggcaa
    33961 aatacgttct cccgcttcta ccagcgttac gttcagggct tcgttggtca ggcctttgta
    34021 accgtagctg tgcagttgct tgatcgcgtt gtgcaattca gcggagagtt ctacacccgt
    34081 cgcgccgccg ccgacaatcg caatgttcac tttgccattt gcgcccaggt tggcagagta
    34141 tttcaggaac agattcagca tctcctggtg gaagcgacgc gcctggtgcg ggttatcgag
    34201 gaaaatgcag ttctctttga cgcctggcgt attgaaatcg ttagaggtgc tacccagcgc
    34261 catgaccagg gtgtcatagg cgattttacg ttccggaacc aacagttcac ctttctcgtc
    34321 gcgtagttct gcaatagtga ttgttttcgc ttcacgatca atatcaatga cggaacccaa
    34381 ctggaactgg aaaccatgat tgcgcgcatg ggccagatag ctcaacgcat cgacgccttc
    34441 atcaagcgag ccagtcgcca cttcgtgcag cagtggtttc cacaggtggc tgtggttacg
    34501 atcgaccagc gtaattttgg cttttttctt acgtcccagc ttatgcccca gctgtgttgc
    34561 catttccagc ccaccagcac cgccgccgac aatcacaatc tttttcaatg gcgtagtcaa
    34621 cgtgaccccc ttaaatttat taaccaattg ttaattaatg gttattaaca tagctcttaa
    34681 ttaacaacag gttacagcaa tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc
    34741 attggtcata ccaaaattga tgttaatcaa gttttgttgc ttaaaagata ggcaacttta
    34801 ccgggggaga tcaaaaaata gcgtttatta tttgagcaaa ttcggaagag taatataggg
    34861 tgaagcgtat atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca
    34921 ttgatgctcg actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg
    34981 catcaggtac cggcaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg
    35041 ctttaatgcg ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc
    35101 agacaatctc gtaataatga tgcaactctt cagatgtccg ctggctgtta agtgcttcat
    35161 cattacgcga caaaataacc agacaacgat cgcgattctt ctcgcggaag ttggtcacac
    35221 acttagtggc aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat
    35281 aagggaacaa attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt
    35341 ccgcccagta tccgcccagg ccaacgccgc aaattagcgg acgctcgtca acgttcagtt
    35401 gcaacatttt gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac
    35461 tgtagcttat caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt
    35521 tacccggact gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc
    35581 cagggttagt gacctgcttt ctcttcctgc cagcgttcat gtaactgatt cagacgggtg
    35641 ctgatcgctt tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga
    35701 tacaaacgtg taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc
    35761 cctttacgat tattgcagac caggatcata tcgcaacccg catccagtga tgcctgcccg
    35821 cgttcggcat aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatc
    35881 acgccgtcaa aacccagttc ctgacgcaaa acggttttca gccagtaggg agaaccgctc
    35941 gccggacgcg gatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta
    36001 ttttcgcgga ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga
    36061 cgtggatcgc acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag
    36121 tgtttcccgg tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt
    36181 gccagggctt tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc
    36241 ccgacatcca gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc
    36301 atcagccaac ccgcctcttg cgccagtttg ccgccctctt ccattcccaa cagcgcagca
    36361 aaggattgtg ctgccggtaa gcgggtaaaa ccttcgcgaa aacgctgcac gcgtccacct
    36421 tcctgatcta ccgccaccac cagatgattg cgcgatgctg cgcggatctg gcgcaccagt
    36481 tcacgtaact gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc
    36541 ggatgcgcca gtatttcacg ctcttccgcg tccagttcgt aaccttcgac atccaacatt
    36601 actggaccca cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc
    36661 cagcctgata aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctccttt
    36721 gagcatcagc agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat
    36781 cttcgcgcga gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca
    36841 caccgccgcc agttccagcg cgatatcacc atctccagca tactcccagt cgatgagttt
    36901 taaccctgat gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa
    36961 gcgtaaaggt cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac
    37021 agtccgccgc gccggatcgc tttgctgcca gtacagttcc agtaagggca acagcgttat
    37081 tcgccagcca aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt
    37141 atccggcaaa tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag
    37201 atataaatgc ggcttcggtg caatgcttgc gggtagttgt gataaagccc gatactggcg
    37261 caagaacgcg gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc
    37321 ctgatgttca atgagaaaac taccgccact aagcccacta ttaaacgtcg tgacgggatg
    37381 aaactgcggg aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa
    37441 cggcaccttt acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta
    37501 gggtcggagc gttaacgttg ccagaggcgc tggagtacag cacgtaatga gcgccgacat
    37561 tgcgggcaat gcctatggct ttactacggg tgcctaaact gtcctgcggc gacaaaccta
    37621 actgttgctt agccatcgac agttgctggg cggaaaccag ggtaaatttc ccgttattag
    37681 ccagcgcatt tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt
    37741 tgttaacgct atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct
    37801 tactgaccat cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg
    37861 gtgccgttcg atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg
    37921 gcactgtggg gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt
    37981 ctaccggtgc aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa
    38041 tcaaggcgta gcgactcatt tttgtcatca agattcaccc ctttacaaat atagataaag
    38101 tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc
    38161 gggaatggta acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata
    38221 ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga
    38281 tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc
    38341 caataaagat gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga
    38401 acgacagccc accagcagta acaacaccag agacaccagc ccaaagcatc cttttctcat
    38461 cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata
    38521 tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag acgatagcca
    38581 tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc
    38641 gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt cggaatgatc
    38701 agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg
    38761 tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc
    38821 acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc
    38881 gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag
    38941 caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg
    39001 ctgaaaagac attctctgtt tggtttacac gacaaatatg aatgcgtata tttctcattt
    39061 gcatttgcaa gcaaaattat tcgcacacta aatataaccg ctcatcaccc tcctccggca
    39121 tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa tttaacaggg
    39181 ataatcaata tcaagccatc accaaaccgt cactacttgc cggttgcata gcactggcac
    39241 tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt
    39301 cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta
    39361 ccaaaatgca gatgactcaa cgtgatattc ctcagtcggt cactattgtt agccagcagc
    39421 ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca
    39481 gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgcggattc cagatcgata
    39541 actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac
    39601 tttctgatat ggcactgttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga
    39661 ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg
    39721 aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg
    39781 atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc
    39841 agaataacga ctcatggctg gaccgctaca atagtgaaaa gaccttcttc tcgggcattg
    39901 tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg
    39961 atgttaatag cccaacctag ggcggtttac cgcgctggaa tactgatggc agcagcaaca
    40021 gttacgatcg cgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca
    40081 aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca
    40141 cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag
    40201 cggatggtat gctggttggg ccatacagta attatggacc tggctttgat catgtcggcg
    40261 gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta
    40321 gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa
    40381 acaatcgtta cttcagttca tgggccaaca tcttcccgga tgaaattggc agtttctaca
    40441 actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggcg caggacgata
    40501 ccacacatat gaaatcgtta tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc
    40561 tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac agcatggaga
    40621 aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac tggtcgacct
    40681 acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc
    40741 tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg atgaatagcc
    40801 gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg
    40861 gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca
    40921 gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag ctgacatttg
    40981 gcgcaacgcg ctatcttgca gaggataacg aaggaaacgc cgttaatcct aatctgccac
    41041 gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag ttgacagtcg
    41101 gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg tatggcacct
    41161 tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga
    41221 aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac gataccaaca
    41281 tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag cattaccggc acgtatcaat
    41341 tctgaataac acctgtaaaa aaggcagcca tctggctgcc ttagtctccc caacgtctta
    41401 cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac
    41461 cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca
    41521 ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg
    41581 cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt
    41641 cgctggtacc tgttgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat
    41701 ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa ccgataccga
    41761 cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgatgaag tcgatcagac
    41821 cgtgcgagaa cgacgtaccg tcacgcattc ccagaagaat acagattggg aatgccaggc
    41881 ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag aagaactcga
    41941 tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca
    42001 ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac
    42061 cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat
    42121 aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca
    42181 tctggaaagg tacgttccag atgtggtgca gaccaaacgg taccagacaa cgttcgatga
    42241 aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct
    42301 ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag
    42361 ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca
    42421 gcttaatacg gtagaaacgg ttaaacatgt acgctgcaat cgcaccggag ataatccctc
    42481 cgagtacgcc agtatccgcc aagtgtttag aggcgatttc ttcagcaggt aaatgcagta
    42541 ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca
    42601 gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg
    42661 gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca
    42721 gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggt agtacggata
    42781 ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa
    42841 ttgagagtgc tcctgagtat aggtgctttt tttacgttct cacgcgtggc aaggggggag
    42901 agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt
    42961 cagtgccaat ttgtttgacg gctatcacgt ttcaacttca gatcattgga gaagttgcac
    43021 attttgacaa aacatatgtc acaatatttc aaacgcggtt attcaccgcc caattttcgg
    43081 cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga
    43141 tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct
    43201 gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa
    43261 ccatcgcagg ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag
    43321 tttccaccag taatcgatcc agggggacat aacgcgcagc atcgcacagt tgctccgcat
    43381 tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg
    43441 ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt
    43501 cgcgcagaat tgccagcgta tcggcacggg cgtcacgggt atggacgata accggcttgt
    43561 tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacgt actttagttt
    43621 ccggtgtgta ataataatcc agcccggttt cacccagcgc tacaacaccc tcttctgccg
    43681 ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac
    43741 aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa taacccggta
    43801 atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt
    43861 catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga
    43921 ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg
    43981 taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtgaca
    44041 gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga
    44101 gaaagatggt ttgccagttc ggcgaccagg cccggcacat caacattggt caccagcgca
    44161 gcgccatgat ggcgttttag cgcatccatc agcaacgttg ccacccagtg taaacgcgcc
    44221 ggagcttgtt catgattaag ggccgccagc agcgaatacc agtcgcccga tggcacgcta
    44281 tatgccaacg cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc
    44341 agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac
    44401 attgtcactt cgcgtgaaag ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa
    44461 tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg ggtagccagg
    44521 aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg
    44581 tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctag gcgtgcgtgc
    44641 tcattcagct tttcggtgac ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt
    44701 tcgggagcca gggtgtagta atcgggatgc gtgccagcct gcatcaactg acatccacga
    44761 cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg
    44821 tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct
    44881 cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat
    44941 ctcatgcgtc caactccttc acccagtggg tcacggtagt gcggattgca tccatcacgg
    45001 cctccagcgg ctgggtggca tcaatggtat gaatactttt atcttgtgca gccagttcca
    45061 gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata cgatccagct
    45121 cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga tagagcgtta
    45181 agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa
    45241 taccacgtcc tccgccctga tacgcctgag tggagagatc gtggcgatcg ccaatcaccc
    45301 aggtgccgtt agccagcgca ggtttgatga ccgtttctac cagttgaacg cgcgcggcat
    45361 aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca
    45421 gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca
    45481 tgtcgcggat acccagttgc tcgagcgtct caaccaccac attacgcgcg gtagttttgc
    45541 ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc cttaagcact
    45601 ttcagataat cctgcacaga cttgttatga ctggcaagat tggtattaaa cgtgtgacca
    45661 cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc
    45721 agcgaatccg cccccggcgt agctatcgca cccggcggca aaccggtaat ggtataggtg
    45781 ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc
    45841 atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata
    45901 aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat
    45961 gccatcgtca ccagctgatt tttatcttta taaggcagac cgtccgcacg cccttcccag
    46021 gcgctatcga ccgctttcac cattttcttg tgagctcgct tgagtaacgc gacatcggtg
    46081 gtattggcgg tatacatcaa ggtgtctggc cagaaccaac cttcaatcca ctccgggttt
    46141 tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt gtgcttgata
    46201 tacggagcct cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc
    46261 agcgggaact gtgcttcttt accgctttcc aacaatttca gcatctcgcg cacggtcatc
    46321 tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc
    46381 agccattgaa aaacccgtgg gcgattgatg atcttatcag cataaagctg ttcaccgagc
    46441 gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg
    46501 ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc
    46561 agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg
    46621 gggctaaata ttcatataac gttgctgacg aaaaggtgac atcgccacag gcacgtacgg
    46681 gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcctcca
    46741 gcgaggcttg cacttcgaca agctgataag aggattgtgc cagtaaacgg atacagaatt
    46801 gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg cgtataaact acattgccct
    46861 tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca
    46921 gcgcctcatc agcgtttgtc tgctcaagat gagagcgaat caatacttgc tcaagccgat
    46981 taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca
    47041 acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg ataagccgta acggagagaa
    47101 tccgcgttgc tggtccgctg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga
    47161 tcacgacttt cagcacacca ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa
    47221 gctgaggcca gaagtcacag gaaatcatca accgctgaca agcatcctgt agtcgctgga
    47281 tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat
    47341 caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttataa ccgttaatta
    47401 agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca
    47461 agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttatatg ggtggaaaat
    47521 gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt
    47581 acacagcgtg tattccattc cgctgacctg acgtgcttca tgcggtacga aatccagatc
    47641 gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc
    47701 caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga
    47761 tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg
    47821 cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg
    47881 actcgcttca atgcctgcat cacgcagagc atttgccatt gccagagctg cgcctgcgcc
    47941 attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc
    48001 agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc
    48061 ggcaccatcg cccagtacga agccatcacg ctctttatcc cacgggcggc tcgccgcttg
    48121 cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg
    48181 cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat
    48241 acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct
    48301 tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga
    48361 cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc
    48421 ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt
    48481 ctcttccgtt atttcaaggc cagaatcctg catggcctga acgccagcga caattccata
    48541 ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa
    48601 atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag
    48661 gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac
    48721 aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca
    48781 aggagggaaa aatgattcta gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc
    48841 ctggagatgt tcacttacgc ctggtggccg ttgatgtaat caatggcagc ctgaacggtg
    48901 gtgattttct cagcttcttc gtccggaatc tcagtatcaa actcttcttc cagagccatt
    48961 accagctcaa cggtgtcaag agaatccgcg cccaggtctt caacgaaaga agcattgttg
    49021 gtaacttctt cctgcttaac gcccagctgt tcgccgataa ttttcttaac gcgttcttcg
    49081 atagtgctca tactcttaaa tttcctatca aaactcgctt tcgcgatggt tttcgtagtg
    49141 tataaaatgt tgaaaaattt gcaactaaat cccggcaggt cttaccacga ttttacgtta
    49201 ttttgaggcc ttacccccca ataacgcaaa tcattttgca ctaattatta ctcataacca
    49261 cgcagactgc gcgcaacatg agcattttgt gcaaatcgcg gtcagaccat gtacatcccg
    49321 ccgttcacat gcaaagtttc acccgtgatg taagctgctt cgtcggatgc caggaatgca
    49381 accgcgttgg cgatttcctg tgcgccgccg aggcgacccg caggaacctg cgccaggata
    49441 cccgcacgct ggtcatcgct cagcgcacgt gtcatgtccg tttcaataaa gcccggagca
    49501 acaacgttta cagtaatacc gcgtgacgca acttcgcgcg ccagtgattt actgaagccg
    49561 atcaagcccg ctttcgccgc agcgtagttg gcctgaccgc catttcccat ggtaccaacc
    49621 acagaaccga tagtgataat acgaccatga cgctttttca tcatagcgcg cattaccgct
    49681 tttgacagac ggaaaacaga tgaaaggtta gtttcgataa tatcgttcca ctcttcatct
    49741 ttcattcgca ttaacaggtt atcacgagtg ataccggcat tattgaccag gatatccact
    49801 tcaccaaatt ctgcgcgaat tttttccaga acagattcga tagatgccgg gtcggtcaca
    49861 ttcaacatca gacctttgcc gttggcacct aaataatcac tgatcgcctg agagccattt
    49921 tcactggtcg cagtgccaat aactttcgcg ccacgggctg cgagcgtttc agcaattgcg
    49981 cggccaattc cgcggcttgc accggttacc agtgcgattt ttccttcaaa attcatgatt
    50041 ttcctctttt aaagctcgag cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg
    50101 gtcagggtgt cgacaatgcg tttcgtcagg ccagtaagca ccttgcccgg gccgacttca
    50161 tagagatgtt ctacgccttg cgctgccatg tactcaacag acttcgtcca ctgaaccggg
    50221 ttatacaact gacgtaccag tgcgtcacgg atggcatcac cattggtttc gcatttcaca
    50281 tcaacgttat tcacaacaga aactgttggt gcgttaaagg tgattttcgc taattctact
    50341 gccagtttgt cggctgctgg tttcatcagc gcacagtgag acggtacgct cactggtaac
    50401 ggcagcgcgc gttttgcgcc agcagcttta caggcagcgc cagcacgctc aaccgcttct
    50461 ttatgaccgg caataaccac ctgtcccgga gagttaaagt ttaccggaga aacgacctga
    50521 ccttctgcag cttcttcaca cgctttcgca atagacgcat catccagacc gatgattgcc
    50581 gccatagcgc ccgtgccttc cggtacggct tcttgcatga acttaccgcg catctcaacc
    50641 aaacgcaccg catcagcgaa atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc
    50701 aggctgtgac cggccatcat tgccggtgct ttaccgccct gctgctgcca tacgcgatac
    50761 agcgcaacag atgcagtcaa cagcgctggc tgggtttgcc aggttttatt cagttcttca
    50821 gctggcccct gctgggtcag cgcccacagg tcgtagccca gcgccgcaga agcttcagca
    50881 aacgtttctt cgacaattgg atagctcgcc gccatatcag ccagcattcc aacggtttga
    50941 gaaccctgtc cagggaacac aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa
    51001 cgaaccagcg cggagcccca agtgaatcca ccgccaaagg cttcaagcag aaccaactgc
    51061 cccggcttaa tgcgcccgtc gcgtacagct tcatccagcg cgcacgggac agaggccgca
    51121 gaggtattac cgtggcgatc cagcgtcacc acgacattgt ccatcgacat accgagtttt
    51181 ttcgccgttg cactgataat acgcaggtta gcctgatgcg gaaccagcca gtccagttga
    51241 gaacggtcaa gattattcgc cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc
    51301 gcaaccttga agacttcgtt gcccgccatc gtcagatgaa ttgaattctc tggattcacg
    51361 cggtcggcgt ttggcagcgt cagcaattca ccataactac cgtcggcatg cagatgggtg
    51421 gaaatgattc ccggctcttc agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa
    51481 ataataatag tcccacgatc tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg
    51541 accagagcat acttcaccgc cccagatttc acgtattgat cggctacgct taatgcatag
    51601 gtgaaacctg cgcaggctgc tgcaacgtca aatgccgggc aacctttaat gcccaacatg
    51661 ctttgaatct gacaagctgc gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg
    51721 atcaggccaa tctggtcttt ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca
    51781 aagcccatgg ttgaaacggt ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta
    51841 cgagtgacaa tccactcgtc agaggtgtcc accatttttt ccaaatcggc gtttgtccgc
    51901 acttgttcgg gcagatagct gccagtacca ataatcttcg tatacatgta cgctcagtca
    51961 cttttcggtt atataccgtc acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg
    52021 cagagttccg cttttgccac cgtccagcag ctcaaaacca gctgggtata cagattccag
    52081 gcgagcggca attcgctgag gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc
    52141 gaccgcaaaa gctcgctgat tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc
    52201 taacagacag gcgccgttat actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct
    52261 cttttgtagc caacgcttta ataacagtag ccaccacgac cgttttttcc cttcaccctg
    52321 agatttcagc agagaaagga acatcctgac aacaccttcc atcgtcttta acgtgacatt
    52381 tcctgtaaag ccgtcacaaa ccagcacatc tgtcttgcca gttaacaact cattggcttc
    52441 aagatagccg atataattga tagaagggat tgttttaagc accgctgagg catcccgaat
    52501 actgtcaaga ccctttactt cttcttcacc aatattgagc aacgccacgc gaggattggg
    52561 aatttccacc acctcttcag ccagaactga gcccataatg gcaaattgca ccaacattgt
    52621 gctgtcacaa tcgacgttgg cccctaaatc gaggaccacc gttttgccct tttgctgatg
    52681 tggtaatacc gtcaccagcg ccggacgctc aatcccctcc aggggcttga gtaataattt
    52741 tgccagcccc atcagtgccc cggtattacc ggcactgaca caggcttgcg ctcgaccttc
    52801 tttcaccagc tccagggcca tacgcattga actcccacga ctggcgcgga tagcttgcga
    52861 aggccgggca tcactggcga taactgactg cgcaggaata atctgcagac gcgaacgttg
    52921 ttcaaagtca gctttagcaa gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag
    52981 agtgagttgc gaattagaat tcagtgcctg caatgctgca ggcactgtca cggaagggcc
    53041 aaaatcccct cccatgacat ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc
    53101 cggtttggtc tttccccagt tgcctgggga aaatcctcac taagcttcat cacgcagacg
    53161 cgtgattact tagcgatgac cttgcggccg cggtagtaac cgtcggcagt gatgtggtga
    53221 cgcaggtgtt tttcaccaga agttttgtct acagacaggc tggtgactgc ggtcagcgcg
    53281 tcatgggaac gacgcatgcc acgtttggaa cgggttggtt tattctgttg tacggccatg
    53341 gaccttactc ctcaattacg gtttatcccc gatccaacgg ggagcaccaa ttacttacgc
    53401 tttaagctgg ctaatacggc aaatgggttt ggcttttgca cttcttcagg cagttcacca
    53461 aagaccatat ccgcttcgga cacttcacag tgttcagaat catgcaccgg aactaccggc
    53521 aaggcgagga tgatttcatc ttcaaccatt gcaagcagat cgatttcacc gaattcgtta
    53581 acctcaatcg gttcatacgc ttccggcagt gcttcagcct gttcgtctga acgcacagga
    53641 ctaaaacaat acgttgtgta gacctgatga gtaaacggct tcccgcaacg ctgacactcg
    53701 agcgttaccg tcaccttcgc atcgccgttt aacactgcga gacgttggtt atcgatagcg
    53761 aacgacatgg agcattccac atcactgtcc acactgacta cggattcggc gacgcgctca
    53821 acctgatcag gggtatagat accctggtaa tcaaggcgtt tttgagccgt acgaaccgga
    53881 tcgagagtca gggggaattt taccttttgc atagggcgcg catattaact ttgtaacgtc
    53941 atagagtcaa agaaaaaggc agcctgtggt tgccttttgc caataattcg cacacattgc
    54001 gggttactgc tttattttcg ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg
    54061 catagtttaa aatggctatc atcaatacgc tatatctgat ggaaaaaaat atgcctaaac
    54121 ttattttagc ctccacctcg ccatggcgtc gcgctcttct ggaaaaactg caaatctctt
    54181 ttgaatgtgc agcacctgag gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt
    54241 tggtgcttcg actggcacaa gaaaaagcgc aatctctggc gtcacgttat ccggatcatt
    54301 taattattgg ttcagaccag gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa
    54361 cggaagaaaa tgcccgtctg caattacgca aagccagcgg caatatcgtc accttctata
    54421 ctggactggc gttgtttaat tcggcgaatg ggcatctaca aacagaagtg gagccttttg
    54481 acgtccattt ccgtcaccta agcgaggcgg agattgataa ttacgtgcgt aaggagcatc
    54541 ccctgcactg cgcgggtagc tttaagagtg aaggatttgg cattacgctg tttgagcgtt
    54601 tagagggacg tgaccctaac acgctggttg gtttaccgct aatcgcgctg tgtcagatgt
    54661 tacgtcggga agggaaaaac ccgctgatgg gatagttgtt tgcgccggat gcggttacgt
    54721 cgcatccggc aatcggtgca ggcggcctga tgcggcgtaa acgccttatc aggccaaccg
    54781 cccattatca ggcttatatg cttgtattag cgcgcgttac gcagtttttg caaacaacgc
    54841 ttcaaacctt catccatcgg cgcttcgata cgcatcacct caccggtccc cggatgggta
    54901 aacttcaacg ctgcggcgtg caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc
    54961 tgtctgtcaa attcacggtc accgtagcga tcgtcaaagg caatcggatg acccgcatac
    55021 tgtgtatgca cacggatctg atgagtacgt ccggttaccg ggctacaacg caccagggtg
    55081 gcaaatgcat agcgttcttc cactttaaag cgtgtttccg acggtttgcc ttcctgactg
    55141 acacgcacga tacgttcgcc gctttgcaga atatttttca ataacggcgc ttgaacgctc
    55201 ttcacatgcg actgccactg accgcgcacc agcgccaggt aatctttttg cattcctttt
    55261 tcacgtaatt gctcatgcag agaacgcaac gccgagcgtt ttttcgctac cagcaacaca
    55321 cctgaagtgt cccggtcgag acgatgaacc agttcaagga accgcgcttc cgggcgcaac
    55381 gcccgcaaac cttcgataac gccgaagctt aaaccactgc cgccatgtac cgccgtaccg
    55441 gaaggtttat tcagcaccag gatatgatca tcttcatata agatgacgtc cgccagcgcc
    55501 gccacctttt gcagatgtgg cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt
    55561 gggatacgca cctcatcacc cgcttcgagt ttatattcag gcttaatacg ttttttgttc
    55621 acccgcactt cacctttacg caaaatacgg taaatcatac tttttggtac gcctttcaat
    55681 tgggtacgca aaaagttatc gatacgttgc cccgcttcgt cagcggtgat agcaacaatt
    55741 tttacggatg gagtctctgt tttcatggtg ggcgattcta attagccaac aggattcgcg
    55801 ccactcattt ttctatgctt atatttagtt ttgcacctta ttacttcact acgtgatcac
    55861 tttattgatg gttattaaac caatcaccag caagaagtga aaaaactgtg agtaagcggg
    55921 tgataaatgg taaaagtcat cttgctataa caaggcttgc agtggaataa tgaggccgtt
    55981 tccgtgtcca tccttgttaa aacaagaaat tttacggaat aacccatttt gcccgaccga
    56041 tcatccacgc agcaatggcg taagacgtat tgatctttca ggcagttagc gggctgcggg
    56101 ttgcagtcct taccggtaga tggaaatatt tctggagagt aatacccagt ctgtttcttt
    56161 gataattgcg ctgtttttcc gcatgaaaaa cgggcaaccg acactctgcg cctctttgag
    56221 ctgacgataa ccgtgaggtt ggcgacgcga ctagacacga ggccatcggt tcacacccgg
    56281 aaaggcgtta ctttgcccgc agcttagtcg tcaatgtaag aataatgagt aagttacgat
    56341 gaaaagaatg ttaatcaacg caactcagca ggaagagttg cgcgttgccc ttgtagatgg
    56401 gcagcgtctg tatgacctag atatcgaaag tccagggcac gagcagaaaa aggcaaacat
    56461 ctacaaaggt aaaatcaccc gcattgaacc gagtctggaa gctgcttttg ttgattacgg
    56521 cgctgaacgt cacggtttcc tcccactaaa agaaattgcc cgcgaatatt tccctgctaa
    56581 ctacagtgct catggtcgtc ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat
    56641 tgttcagatc gataaagaag agcgcggcaa caaaggcgcg gcattaacca cctttatcag
    56701 tctggcgggt agctatctgg ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg
    56761 ccgtatcgaa ggcgatgacc gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc
    56821 ggaaggcatg gggcttatcg tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca
    56881 atgggattta agcttccgcc tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg
    56941 cccggctccg ttcctgattc atcaggagag caacgtaatc gttcgcgcct tccgcgatta
    57001 tttacgtcag gatatcggcg aaatccttat cgataacccg aaagtcctcg aactggcacg
    57061 tcagcatatc gctgcattag gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg
    57121 cgagatcccg ctgttcagcc actaccagat cgagtcgcag atcgagtccg ccttccagcg
    57181 tgaagtccgt ctgccatccg gtggttccat tgttatcgac agcaccgaag cgttaacagc
    57241 catcgacatc aactccgcac gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa
    57301 caccaacctc gaagccgccg atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg
    57361 cctgattgtt atcgacttca tcgacatgac gccagtacgc caccagcgtg cggtagaaaa
    57421 ccgtctgcgt gaagcggtgc gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg
    57481 ctttggcctg ctggaaatgt cccgccagcg cctgagccca tcactgggtg aatccagcca
    57541 tcacgtctgc ccgcgctgct ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct
    57601 ctctattctg cgtttgatcg aagaagaagc gctgaaagag aacacccagg aagttcacgc
    57661 cattgttcct gtgccaatcg cttcttatct gctgaatgaa aaacgttctg cggtgaatgc
    57721 gattgaaacg cgtcaggacg gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac
    57781 cccgcactac cacgtgttgc gcgtgcgcaa aggggaagaa acgccgacct taagctacat
    57841 gctgccgaag ctgcatgaag aagcgatggc gctgccgtct gaagaagagt tcgctgaacg
    57901 taagcgtcct gaacaacctg cactggcaac ctttgccatg ccggatgtgc cgccagcgcc
    57961 aaccccagct gaacctgccg cgactgtcgt agccccagca ccgaaagctg caacggcaac
    58021 accagcagct cctgcacaac ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct
    58081 gttcagcggt ggtgaagaag ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa
    58141 accggaacgt caacaggatc gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga
    58201 gcgccgcgac acccgtagtg aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg
    58261 taatcgtcgc caggcacagc agcaaactgc cgagacgcgt gagagccgtc agcaggctga
    58321 ggtaacggaa aaagcacgta ccaccgacga gcagcaagcg ccgcgtcgtg aacgtagccg
    58381 ccgccgtaac gatgataaac gtcaggcgca acaagaagcg aaggcgctga atgttgaaga
    58441 gcaatctgtt caggaaaccg aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa
    58501 acagcgtcag ctcaatcaga aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt
    58561 cgcaccggtg gttgaagaaa ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc
    58621 acgcacagaa ctggtgaaag tcccgctgcc agtcgtagcg caaactgcac cagaacagca
    58681 agaagagaac aatgctgata accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc
    58741 gcctcgtcac ctgcgcgtga gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc
    58801 aacccagtcg ccaatgccgt tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa
    58861 agtctggatc cgctatccaa ttgtacgtcc gcaagatgta caagttgaag agcagcgcga
    58921 acaggaagaa gtacaagtgc agccgatggt gactgaggtc cctgtcgccg ccgctgtcga
    58981 acctgttgtt agcgcgccag ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc
    59041 ggttgccgaa ccgcaaccgg aagtggttga aacgacgcat cctgaagtga ttgccgccgc
    59101 ggtaactgaa cagccgcagg tgattaccga gtctgatgtt gccgtagccc aggaagtcgc
    59161 agaacacgca gaaccggtgg ttgaaccgca ggaagagacg gcagatattg aagaagttgc
    59221 cgaaactgct gaggttgtgg ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt
    59281 cgtcgctgaa gtcgcagcag aagttgaaac ggtagccgcg gttgaaccag agatcaccgt
    59341 tgagcataac cacgctaccg cgccaatgac gcgcgctccg gcaccggaat atgttccgga
    59401 ggcaccgcgt cacagtgact ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc
    59461 aggtggtcat acggcaacgc atcatgcctc tgccgctcct gcgcgtccgc aacctgttga
    59521 gtaataatta gctcaaagta atcaagccct ggtaactgcc agggcttttt tatttcatgt
    59581 ttgaatcaac atattaaaat tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag
    59641 gcaaaaaaaa tgcccgcccc ggcgatccca gagcgggcag acaaaacata cccagtttca
    59701 tgatatgttt taaagcgagc ttatttgttg agctggaaga gcgacaatcc ctgcatatcg
    59761 gtaaatgctt tatacgatgc ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt
    59821 gcattccagt ccacatcaac cagatcgctc atctgctgcg tttgccctaa agcgcgatcg
    59881 ctacctaagg aatccagcga ctccagtttg ttcagctgcg tgcctaattc cgtgcgaacg
    59941 gtaaggacat tattcagcga gtttttcagt ccacggttgg ttttatccaa tgccgcagcg
    60001 gcgatttctt tatccgcttc gctatccgcg accggcgttt tcagggctgc gatggcgcta
    60061 tccagcatgg caaaaagatt ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta
    60121 ctggtaatac tgtcgaaaat tttgtcgccc gtatgtccta tcaccatcga acgcgaagca
    60181 tcgacctgtt gcttaatact ttctgttccg ccgacataat caccgttagc ctcactaaac
    60241 ggcgcagtct ctgttttata accggcaaaa atgtagcgcc cattaccgtc agtggtgttc
    60301 gccagattca gcaactggtc acgaagcccc tgaatatccg tagccagcga ggcccggtca
    60361 tcgtcactca aggtgccatt gctggcgtag acaatttttt cctgagcatt ctggatagca
    60421 gtggtgacct ggctaagtac actctcttcc agtgacactt tttgagtggc gaaagtacgt
    60481 gccagcgtgt actggctgtt ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca
    60541 gcaatgggat cgtctgaagg gttaacgact cgcttacccg tcgacatctg ctcgccgtac
    60601 ttcatccatt ctgcctgaga attggtgatg ccgcgcatgt tttgctggta catcatctgg
    60661 gtactgaaac gcattttatc tccccttagc gaatgttaat cagcgcatca aaaatcgcgt
    60721 ttgccgtctg cagaacctgc gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc
    60781 cgtattcctc atcgagattg acaccggaaa tcgactgctg ctgattggaa agctgcgtca
    60841 ccacattacc ttgcgtggtg ctactggttt tcaacgtcgc ggttttatta ccgatatcac
    60901 tcactaacga ggcataagcg tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc
    60961 tttgcagatc cagcagggcc tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc
    61021 tcgccatcgc gattttcgct tcgtcggtga ttaatacatc catgttgacg atggcgtcac
    61081 ttactggttt cagcgtgaag ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca
    61141 gaccatcaaa tgccacttta ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg
    61201 caaggcgggt gacctgccac tgattattat cgaacgagat tttgtaatct gtcgccagta
    61261 ccacggaggc atcagttacc gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt
    61321 gctgaaccgc gggattaccg atagtaaaga aatcttcacc agcatcgccg ttagcatcaa
    61381 atccggcttt gtgttggctg ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg
    61441 tattacgcgt ctggtccaga tcctgagaac ggaatgtcag aatgccgccc agcgacccgg
    61501 tattcagtaa tttctccggg atctcaatat tgcctgccgt cccatcaacg taagcgacag
    61561 tcgtacgaga agggtcggcg ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct
    61621 gaaccagtga gtaaccattg gcatcgtgat gttataagta ccgccatcct gaacgctgac
    61681 ttctacgcca acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt
    61741 gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt tcaggctggc
    61801 aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg
    61861 tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga
    61921 tttcccaatc agcgcctggc gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag
    61981 gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt
    62041 gtcgattttc gacatctgct catagcgggc ggtcaggccg ctactttgcg tctgcacagc
    62101 acgtaactgg ttggtaataa acgtatcata ctcacgctgc acaccagaaa cgtagacgcc
    62161 attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg
    62221 gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc
    62281 ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta
    62341 taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac
    62401 agattatcaa tgttcatgct gtaggttttg ctcacctggt cgcttatcga tttcatctgc
    62461 tggatcatgt tggtgagttt acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt
    62521 aggacctgcg ccccctgttc cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta
    62581 cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaac
    62641 ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat ttcagtgact
    62701 tgccctttcc agttgccaga ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt
    62761 tcgcggcgga tttgccgttg cccccaacca gattcaagcg cagcctgagc gaggatcaaa
    62821 tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg
    62881 aatgctctac tgtcacccgg cagcgaatca tcgtagttac gtggtacggc cttttgcacc
    62941 agctgcgaaa gcgtctggtt ttgataacgc accacggttt cgagcgggaa tttcatcggt
    63001 gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc
    63061 tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatttgctg gtcatacata
    63121 ctggtataca gacgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc
    63181 atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata
    63241 tttgccgccg gatcttcgct cgctttcgcc tttagttcgt tgagtgattg cgcatcccag
    63301 gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca
    63361 gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc gtagcgccca
    63421 gcgcattgag cgcacgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg
    63481 aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aacaacagtc tgtccaccac
    63541 caaacggtgt atctggctgg ctgacattgg cctgacgatt aactgttact gagagatttc
    63601 cctgcgctac cgcgcagctg tcgagggtca cttctcgatt catcaccacc gaaccggtgc
    63661 gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat
    63721 cggcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg
    63781 catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct
    63841 gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact
    63901 ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt
    63961 taacctgaac actgctaccg cccgcggagg ctcctgcgcc gccaaccaga atattgccct
    64021 gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg tgtcatcaac aacgtacctc
    64081 cacgcaagct tttggcatta cccatggaag aaaccaccac atcgatggtt tgcccctgac
    64141 gtccaaacgg aggaagtgac actgtcacca ttaccacagc gacgtttttt agctgcatat
    64201 tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg
    64261 taaacggcgt ctgggttgtc tggtcaccag tgccatccag ccccactacc aggccatagc
    64321 caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct
    64381 gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct
    64441 cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc
    64501 gttaatgtag ccattgccta cgtattcaat acgcgcatcc gccacctgag tagacggtac
    64561 ggtattgctg ccgctgatag tgcgtggatt aaccacgccc gagaagcgaa taaattcggt
    64621 accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac
    64681 ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt
    64741 tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc
    64801 ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt
    64861 cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc
    64921 aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc
    64981 agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt
    65041 cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa
    65101 caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa
    65161 tcagtaaacc ggtgaacctg ccaccggtta tgccttagag ttgcgtcagt ttttgcagca
    65221 tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca
    65281 tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca
    65341 gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg
    65401 tgtagaggtt ttcgccaatg ctctccagtc cggtatcatt cataaaggtg gtgagattga
    65461 gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac
    65521 gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac
    65581 caccagccgt caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg
    65641 ctgatgagcc atctggcaac attacctgga aaaagccctg ccctttaatc gcgacatctt
    65701 tgctgttatt ggtctgcgac agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc
    65761 gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc
    65821 ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac
    65881 cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt
    65941 caaggcccgt tttggcgatc cataatgaac tgatcatata atgtcctgta gtggattgct
    66001 gacaagcaga gcagaagcac gcgacagtgt gaatttcccc tcaccctaac cctctcccca
    66061 aaggggcgag ggaatagtcc gcgcgctttt attgcaatgt catcaatcgt tcaattaact
    66121 catcgacagc agttggttgg cacggcctgc gttatcatcg acgctgctga tcaccttcat
    66181 ctgcatttca aaacgccgcg cgctggcaat catgtcgctc attgccgcaa cggcattgac
    66241 gttactgcct tccagaaccc ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg
    66301 ccctcgcgtg gcctgggttt ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac
    66361 ttcgctgccc gtggctttca ccagtttcag acgccctact ggcgcaactg tatttgccgg
    66421 atcgcccgga ttgagcgccg agattgtgcc atcggcagca atcgtgattt ccgccccttc
    66481 cggcacagca attggccccg cctcgcctat caccggatgc ccctgaattg tcagttgccc
    66541 ggtgggatca acctgaatgc tgccattacg cgtataccct tcgctgccgt cagcggtctg
    66601 cacggccagc catcctgctg caacgcaacg tccagcgggc gcgaggtgta atccatcttg
    66661 ccgggcgtca tgtctgcacc cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa
    66721 agcccttcca ccggcactgc gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag
    66781 gcattggcca gattactggc ggttaccgcc tgttgattca gcgtctggct ggctgctccc
    66841 atcgcagtat aaattgcgtg atccattgag ctatcccgtc agcgattagc gtaagttaac
    66901 ccgcgtgttg aggatctggt cctgggtttt gatggtctgg gcgttagact gatagttacg
    66961 ctgggcaacg atcatattga ccagttcttt actgagatcg acgttggacg cttccagcgc
    67021 accgttggtc agggtgccaa agtttcccgt cccggctgtc cccaacagcg ccacgccaga
    67081 agattgcgtc gcagaccaga cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa
    67141 gttcgccagt acaatctgcc ccagcagttg ggtttgttcg ttggaatagt tgccgacaac
    67201 cgtaccgtca tcattgattt gataactcac cagatcgccc ggtttgtagc cattctgggt
    67261 ggttgccaca atgttgttag cgccggtatt ttgctgcatg gagttgagga agctcagact
    67321 aaacgtggcg ggttctgcac cgttaattgc gccggttgcg atattattcg ccatcgcaca
    67381 tgtgccatta gcattaaatt ccagtgttgt cgctgtcttc gcaatgctgt ttggatcact
    67441 gctatcctgg gtgtagacct gccagttatt atccccggtc ttcacaaaat agacgctcat
    67501 gtcatgagca ttaccctgac tgtcgaaaac agtcaccgaa ccttttttgt tatagctatc
    67561 cgcattgctg gcgctgaatg gcgtaacagt aggaagcgga tcactggaat tcaggttgat
    67621 ctgcatcgac gccgtggtgg tagttttcgc tgccatcagg gtattcggga tcgaaatatt
    67681 ggtcggattc gccccttgct gaatagtcgg cggcgtaccg gttgccgggt aacccgtcag
    67741 ctgtaaacct tgcatattca acaggttacg gttttcatcc agcttaaatt gtccgttacg
    67801 gctgtagaac accgaaccgt tgctgtctac cagacggaaa aaaccgttct ggctgatagc
    67861 aacgtccaga cctcgcccgg tgttggtggt cgtgccatcg gtaaagtcct gagtgatacc
    67921 ggcaactttt acccccagtc ccactttcga accggcaaac atatcggcaa aagaggccgt
    67981 gcctgattta aagccgtagg tggcggagtt ggcgatattg ttgccaataa catcgaggtt
    68041 ggtggcggca gcgtttaatc cgctaaccgc ttgagaaaag gccatgactg actcctgaaa
    68101 tgtgaaggct taaattatct gccgtacttc gtcgagggtg gtggtgccgt aagtgccgag
    68161 atccagcgta ttaccgctgt tgccgcggat cacaccctgc accagagcaa actgcagcgg
    68221 ctgggcaacc agttgtgtac caccgttact ggcgctaatc gctacattgt aagaaccgtt
    68281 cggcgcagtg ctgccatcag tcaacgtacc gtcccaggtg aaactgtgaa ctccggcggt
    68341 cagttcacca atatcaatgg tgcgcacaac cgcgccattt ttatcggtga tggtggccgt
    68401 aactttgtct gccgcctgtt gcagctcaac accaaacggc gtggtcgtgg tcacagcccc
    68461 ttcttcactg ccggttcccg caagaacagt tgtgccgggg atcatcacgc cgtgaccgat
    68521 caggttactg gcctgtaacg actggctgtt atcaatctgt ccggaaatag atccgagcgt
    68581 ggtattgagt ttttcaatcc cgctgaccgt gctgatttgt gccaattgcg acgtcagctc
    68641 gttgttttcc attggattgg tcgggtccta gtttttcagc tgcgccacca gcaaagtcag
    68701 aaaactgctt tgtaaatctg cggcgttgct gcccgtgagc gaactactgc tggtggtact
    68761 gacgccggta tttgtcggat cggtggtggt taccgcaatg gacatagctt tctcctttat
    68821 tgaccgagcg taagggtttt cagcatcatg cttttcaccg tgttgagcac ttcaacattg
    68881 gcctgatagc tgcgtgacgc cgacatggtg ttaaccatct ccccgacgac atcaacgttc
    68941 ggcattttta cgtagccctt tgcatctgcc agcggattac ctggttcata aaccagtttg
    69001 tccggggcct gactttctta acatcggcaa cctttacgcc gcctgtcgca gcacctggtg
    69061 cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca ggaccggtca
    69121 cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta
    69181 acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta
    69241 aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca
    69301 ggctgttatc ggcaaactgg gtgacttcct gacgctgggc gcgcagattg agcgcctctt
    69361 gttgaaaacg taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat
    69421 tctgccatca gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg
    69481 tcgctattta tgccgttgat ggtcattgcg gacaggtaca actcacgttg tagaaatggc
    69541 tgggggcgaa aatgctgaca ataaaacgta gcgtggcgat catcgcgata ctgttcagtc
    69601 cgttaagtac ggcgagcaat ctcacatcgc aattgcacaa cttttttagc gcccaactcg
    69661 cgggggtaag tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat
    69721 gcgagcagcc attgctttcg atgagcaata attcccgcct gtggggcaat gtgaatgtgt
    69781 tggcacgctg cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt
    69841 atgtggttgc cgcgatgcct attgtgcggg gaggaaagct ggaagctggc aatgtcaaac
    69901 tgaaacgtgg acgcctggat accctgccac cgcgtacggt gctggatatc aatcaacttg
    69961 ttgatgccgt tagcctgcgc gatctatcac ccgatcaacc tatccagtta acccagtttc
    70021 gccaggcatg gcgggtaaaa gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt
    70081 ttagcgccaa cgcagaaggt caggcgctga acaatgcagc cgtcgcacag aatgcgcggg
    70141 tgcgcatggt atcgggacag gtagtcagcg gcgttgttga tgcagatggg aatattctta
    70201 taaacctgta agctgttaaa gattacccgt cccttgccga taaataagca acacatgata
    70261 aaagcgccct caatgaggaa taaaccatga gtattgatcg cacttcgcct ctgaagcctg
    70321 taagcaccgt tcaaccgcgc gaaaccactg acgcgccggt aacgaacagc cgcctccacc
    70381 agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga tgcaacccgg cagcagtgat
    70441 atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc gtaacggtga actaaaaatg
    70501 gacaccggca aaattgccga tgcgctgatc aacgaagcgc agcaagactt gcagagtaac
    70561 tgatcgtatg acacgtcttg cagagatcct cgaccagatg tccgctgtgc ttaacgatct
    70621 caaaacggta atggatcatg agcagcaaca tctctctatg gggcagatca acggcagcca
    70681 gttgcaatgg attacagaac aaaaaagctc actgctggcg acgctggatt acctcgaaca
    70741 gttacgcaag aaagaaccca atacagcaaa tagcgttgat attagtcaac gctggcagga
    70801 aattactgtg aaaacgcagc aactacgcca aatgaatcaa cataacggct ggttactgga
    70861 aggacagatt gagcgcaatc aacaggcgct ggaaatgttg aaaccgcatc aggaaccgac
    70921 gctatatggg gcgaacggtc agacctcaac accccatcgc ggcagtaaaa agatttcgat
    70981 ctgaatatta tctccggcct gcactgcagg ccggaatgca ttgttacacc gtccggcggg
    71041 caaattcttt aactttgaag cccagtaccg ccagtgcagc gaagtacgcg gcaatccccg
    71101 ccagcacgac cgccattaaa cgcagtaagc gccagggcat ggtacccaat gaccactccg
    71161 gcatgatatg taacatacct aaaagcacgc cagacatcac cagtaccgcc accaccagac
    71221 gcaacagaaa cgccatccag ccgggttgcg gagtaaagat tttctgctta cgcaactgcc
    71281 agtaaagcag cgaagcattc agacacgccg ccagaccaat agaaagtgac agcccggcat
    71341 gtttcaacgg accaataaac gccaggttca tcaattgcgt taaaatcagc gtaacgatgg
    71401 caattttcac tggcgtttta atgtcttggc gggaataaaa gccaggagcc aacactttca
    71461 ctacaatcag gccaatcaaa cccaccgagt aggcaattaa cgcccgctgg gtcatcagcg
    71521 catcaaacgc ggtaaattta ccgtactgga acagcgaaac ggttaacgga cctgaaagaa
    71581 tgcccaacgc aaccgcactc ggcagtgcca acaggaaaca aagacgcaac ccccagtcca
    71641 tcaaacggtt gtattcatca tgattgccac tggcaaaact tttcgacagc gacggcagca
    71701 aaatggtgcc aagcgccacg cccagcacac cggagggaaa ctccattaag cggtcggcgt
    71761 aatacatcca agacaccgaa ccggaagcaa gaaacgaggc aaaaatggtg ttgatgatta
    71821 aggagatctg gctcacagag acgccaagga tcgccggtcc catctgtttc accacgcgca
    71881 ttgctccagc atcgtggaag ttaatgcgcg gcaggaccag catgccgatc ttcttcaggt
    71941 gcggtagctg atacaccagc tgcaggatgc cgccgaccgt aacagcccac gccagcgcca
    72001 gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa accaatcatg ctgatgttaa
    72061 gcagtgttgg cgcaaacgcc ggaatcgaga agcggttcca cgtattcaga atcgctccca
    72121 ccagcgacgc cagggagatc agcaagatat agggaaaggt aatctttagt aggtggctgg
    72181 tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc ggtcaccatg atcacccacg
    72241 gtgcggcgag catgccagcg accgtcacaa ccgccagcgc aagtgtcagc aggccagaaa
    72301 cataagagac aaagacccgc gtggcgtctt caccctgctt acttttatat tccgccagaa
    72361 tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat acggcgtaac aagttaggaa
    72421 gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc gccaaagatt ctggcgacaa
    72481 ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt catcgagctg acggcggcca
    72541 gcgattttaa taaattcatc ggtgttctaa tccggaccca cgggcaaaac gcctgcaagg
    72601 caggcgctga aagaggcgaa tagtctacct agattatggt gaattgctac cgccagatgt
    72661 tacagggttt attcactcat cgcatcgcgc cagatcttgt caacgatacg ttgcgccagc
    72721 actgcctatt cgccggcggt ttgcggaact gtctgatttt gcacgcattc aatgaagtgc
    72781 cgcgcacagc cgacaaatcc acgttgctca agagtgctct gccaaccagg aatcggttta
    72841 ttcactacac cctgcccgcg ctcctcacgc cattcgcgca tatccgtaat gtcgatgagc
    72901 gcaccgtcag tcacggcctg cacggtttca cgctgacttc cggcacggcg atgcatacaa
    72961 gtggtgatct gcaaaggacc ggccgaaaaa tggtgctcgg caaacagcat ttcgccagcg
    73021 tcgttagtca gtagcgtacc gccatccaga gaggctttgc cgcccgacaa ccacagcgcg
    73081 gtatccacca catgcagata atcatccagc aacgtgaaat aaagatcgtg tggcccgacg
    73141 ctattgctac gatgtttatc cattcttagc gaggctgcgg tggcgagttg cgtttttaac
    73201 tcaccgtaga gtggtgcgaa acgacggtta aaaccgacca tcaacgtcag ttttttccgc
    73261 gccgccagtt ccaccagcct ttcagcatcg cgcagatttt ctgccagcgg tttatcgaca
    73321 cagacatgta cccccgcatt gagtaacgta ctgaccacgt caaagtggct ggcggtgctg
    73381 gagtgcacaa aaaccgcatc acaactggcg gcaaggctgg ataacgaatc ggcataagga
    73441 atgcgccagc tgtcacaaat tggcagggct ttggcgcgcg taggcgacca ggctccttgt
    73501 aacgtccagt cagacgctgc cgccagcacc ggtaaccacg ctttttgcgc aatgccgcct
    73561 aatcccacta cgccgatacg taattttttc actttaatct cccagatggg ccagcaacga
    73621 atcaagacgt tgtttcagtt ctgccacttc gatttccagg gcttcgacgc gggtctgtaa
    73681 atcaccgtca accatgttcg acatagccgt taccgccggc tgatcttcaa cctcaccact
    73741 gaaaagatgc atgtagcggc tttcgcgttt acccggttcg cgggccagac gcaccacaaa
    73801 aggaccatct tcgcgattcg ccagttgttc cagcgtcgac tccacttccg ccatatcgct
    73861 gaattcatac attcgcgcgg cgcggctgcg cagttcaccc agcgtctggg caccacgcaa
    73921 taacaacgtg gtgattaacg ccacttctgc ggcgctcagt ttcagatcgc caaattctga
    73981 attacaaaaa cgttgctcat atttggtgac ccgattacca aaaccgctca ctgtgcgtag
    74041 ataatgacgt ttgaccagat tatccagctg ttcctgcact tcggattcac tcagattcat
    74101 gaccggttca cggttcgttt tctgattaca ggccgtgact acaccattga ctgagagcgg
    74161 gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa ccaattacgc gagcttccag
    74221 ggcagtaagt tgatatttca tgagattctc cttagcggcc gggcgtccag tctggggtag
    74281 ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat caacagatag tctttcgcat
    74341 agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc gctgcgttta ttgtgcggca
    74401 tataattagc cataatgcga tgaatatgtt gggtgcgctg catataacga atggctgcgg
    74461 tcagggcttc aaacatgagt cctttgccct gccatttttg cccaatcgaa taaccgagat
    74521 agcaggcatg aaaagagccg cgaacaacat tggaaaaatt agcaacgcca ataatctctt
    74581 tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc ttgtttatga aattcgttaa
    74641 tcatccctag cctggcctgc cagcctgatg gataacagtg gctttcgtcg cgcactggct
    74701 cccagggctt gaggaaatgg cgattctctg cgtaataatc cgccagacgc caggcatcac
    74761 gatcatgcac cagacgcacg accagtcggt ctgtggttaa gcgcactttt ggcacgttac
    74821 tgcgatagcc aaacattcta tacctactcc ttcccgtaac gtctttaata cgctgtaagt
    74881 attactatac ctgcgcaacc agaccctgtg aaaagtccaa aatgccattt tctgcattat
    74941 tcacatattt gataaaaact ctctatcaaa gcgacgtttt gataaaaaaa tattgtcccc
    75001 gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa agtgctcatc ttttatttcc
    75061 cgggagggga aatgtcccgc gtatcgcagg cgaggaacct gggtaaatat ttcctgctca
    75121 tcgataatat gctggtcgtg ctggggttct ttgttgtctt cccgctgatc tctatccgct
    75181 tcgttgatca aatgggctgg gccgccgtca tggtcggtat tgctctcggt ctacgccagt
    75241 ttattcagca aggtctgggt attttcggcg gcgcaattgc cgaccgcttt ggtgccaaac
    75301 cgatgattgt taccggtatg ctgatgcgcg ccgccggatt cgccacaatg ggtatcgccc
    75361 acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg actcggcggc acgttgtttg
    75421 atccgccgcg ttcggcgttg gtggtgaaac taatccgtcc acaacaacgt ggtcgttttt
    75481 tctcgctgtt gatgatgcag gacagtgcca gtgcggtcat tggcgcattg ttggggagct
    75541 ggctgttgca atacgacttt cgcctggtct gcgccacagg ggccgttttg tttgtgctat
    75601 gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact ctccaccgta cgcacgcccg
    75661 ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt tgtcacctat gtcctgacgc
    75721 tggcgggtta ctacatgctg gctgtacaag tgatgctgat gctgccaatt atggtcaacg
    75781 acgtggctgg cgcgccctct gccgttaaat ggatgtatgc cattgaagcg tgtctgtcgt
    75841 taacgttgct ctaccctatc gcccgctgga gtgaaaagca ttttcgtctg gaataccggt
    75901 tgatggctgg gctgttgata atgtcattaa gcatgatgcc ggtgggcatg gtcagcggcc
    75961 tacaacaact tttcaccctg atttgtctgt tttatatcgg gtcgatcatt gccgagcctg
    76021 cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc tcgcggcagc tatatggggt
    76081 ttagccgtct gggtctggcg attggcggcg ctattggtta tatcggtggt ggctggctgt
    76141 ttgacctggg caaatcgacg caccagccag agcttccgtg gatgatgctg ggaattattg
    76201 gcatcttcac tttccttgcg ctgggttggc agtttagtca gaaacgcgcc gcgcgtcgtt
    76261 tgctggaacg cgacgcctga tttgatacac ccccctgact cctccatact gaaagtgaac
    76321 tgacagccaa atggaggagt cacgtgaagc tatacattta cgatcactgc ccttactgcc
    76381 tcaaagcccg catgattttc ggcctgaaaa atatccccgt cgaattacat gttctgctca
    76441 acgacgacgc agaaacaccc acccggatgg tcggtcaaaa acaggttccc attctgcaaa
    76501 aagatgacag ccgctatatg ccagaaagca tggatatcgt tcactatgtc gataaactcg
    76561 acggcaaacc gttactgacc ggcaaacgtt cccctgccat tgaagagtgg ctgcgcaagg
    76621 tcaatggcta cgccaacaaa ctgctgttgc cgcgttttgc caaatcggca tttgatgagt
    76681 tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa agaggccagc gcgggtaatt
    76741 ttgccgacct gctggcccac tctgacggtc tgattaagaa tatcagcgat gatttgcgtg
    76801 cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg cgaactttcg gaagatgata
    76861 ttcagctatt tccgctactg cgtaatctga cgctggtagc cggaatcaac tggccaagcc
    76921 gcgttgctga ttaccacgat aatatggcga aacagacaca aatcaatttg ttatcatcaa
    76981 tggcgattta atcctgaccc gccgcggcag cctgtattcg cgggttgccg ccgttttcag
    77041 ttttttctgc tggcgagttt atcgctggtg acacatgctg cgttgtgtca ggatattgac
    77101 gcagatttca tgtcgaggac taccatgaac aagtttttat ttgccgctgc attgatcgtc
    77161 agtggcctgc tcgttggctg taatcaactc acccaataca ccatcaccga acaagaaatt
    77221 aaccagtcgc ttgcgaaaca taataatttc tcaaaagata tcggtttacc cggcgtggct
    77281 gacgcccata ttgttctgac aaacttgacc agccaaattg gtcgcgaaga gccgaataag
    77341 gttaccctaa ccggagacgc caatctggac atgaactccc tgttcggtag tcagaaagcg
    77401 accatgaaac tgaagctgaa agcgctgccc gtgtttgata aagaaaaagg tgcgatcttc
    77461 ctgaaagaga tggaagtggt cgatgcgacg gtacaaccgg aaaaaatgca aacggtgatg
    77521 caaacgttgc ttccctattt gaaccaggca ttacgcaatt actttaacca gcaacctgct
    77581 tacgtcctgc gcgaagatgg cagtcagggc gaagcaatgg cgaaaaaact ggcgaaaggc
    77641 attgaagtga agccaggtga aattgtcatt ccgtttacgg attaatgacg agggcgcatt
    77701 cgcgcccttt atttttcgtg caaaggaaaa catttccgct tatcctttgt gtccggcaaa
    77761 aacatccctt cagccggagc atagagatta atgactgcac catcccaggt attaaagatc
    77821 cgccgtccag acgactggca ccttcacctc cgcgatggcg acatgttaca aactgtcgtg
    77881 ccgtatacca gcgaaattta tggacgggct atcgtaatgc ccaatctggc tccgcccgtg
    77941 accacagttg aggctgccgt ggcgtatcgc caacgtattc ttgacgccgt acctgccggg
    78001 cacgatttca ccccattgat gacctgttat ttaacagatt cgctggatcc taatgagctg
    78061 gagcgcggat ttaatgaagg cgtgttcacc gctgcaaaac tttacccggc aaacgcaacc
    78121 actaactcca gccacggcgt gacgtcaatt gacgcaatca tgccggtact tgagcgcatg
    78181 gaaaaaatcg gtatgccgct actggtgcat ggtgaagtga cacatgcaga tatcgacatt
    78241 tttgatcgtg aagcgcgctt tatagaaagc gtgatggaac ctctgcgcca gcgcctgact
    78301 gcgctgaaag tcgtttttga gcacatcacc accaaagatg ctgccgacta tgtccgtgac
    78361 ggaaatgaac ggctggctgc caccatcact ccgcagcatc tgatgtttaa ccgcaaccat
    78421 atgctggttg gtggcgtgcg tccgcacctg tattgtttac ccatcctcaa acgcaatatt
    78481 caccaacagg cattgcgtga actggtcgcc agcggtttta atcgagtatt cctcggtacg
    78541 gattctgcgc cacatgcacg tcatcgcaaa gagagcagtt gcggctgcgc gggctgcttc
    78601 aacgccccaa ccgcgctggg cagttacgct accgtctttg aagagatgaa tgctctgcag
    78661 cactttgaag cattctgttc tgtaaacggc ccgcagttct atggcttgcc ggtcaacgac
    78721 acattcatcg aactggtacg tgaagagcaa caggttgctg aaagcatcgc actgactgat
    78781 gacaccctgg tgccattcct cgccggggaa acggtacgct ggtccgttaa acaataaaaa
    78841 tcgtagcttc ctgttgtcat taggttattt tacctgtata aataaccagt atattcaaca
    78901 gggggctatt atgcgaattg aagtcaccat agcgaaaact tctccattgc cagctgg
//
LOCUS       DADLTF010000004        66599 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.004, whole genome shotgun sequence.
ACCESSION   DADLTF010000004 DADLTF010000000
VERSION     DADLTF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.004"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN749_000273"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="JN749_000273"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HAY7366808.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN749_000274"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="JN749_000274"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HAY7366809.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN749_000275"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="JN749_000275"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HAY7366810.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN749_000276"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="JN749_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HAY7366811.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN749_000277"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="JN749_000277"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HAY7366812.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDASHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN749_000278"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="JN749_000278"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAY7366813.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="JN749_000279"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="JN749_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HAY7366814.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN749_000280"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="JN749_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HAY7366815.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMDICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLN"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN749_000281"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="JN749_000281"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAY7366816.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN749_000282"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="JN749_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HAY7366817.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /locus_tag="JN749_000283"
     CDS             complement(14847..15542)
                     /locus_tag="JN749_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7366818.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="JN749_000284"
     CDS             complement(15665..16702)
                     /locus_tag="JN749_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7366819.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="JN749_000285"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="JN749_000285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7366820.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19270)
                     /gene="ggt"
                     /locus_tag="JN749_000286"
     CDS             complement(17582..19270)
                     /gene="ggt"
                     /locus_tag="JN749_000286"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HAY7366821.1"
                     /translation="MLLCSQEVVLAPPVSYGVEEDVFHPVRAKQGMVASVDATATQVG
                     VDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKNGNTTAIDFREMAPAK
                     ATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGTMPLNKVVQPAFKLAR
                     DGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGGTLVQANLAKSLEMIAE
                     NGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPISGDYRGYQVYSMPPPS
                     SGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADRSEYLGDPDFVKVPWQ
                     ALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTTHYSVVDKDGNAVAVTYTL
                     NTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANAVGPNKRPLSSMSPTI
                     VVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNAPRFHHQWLPDELRVE
                     KGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDPRSVDDLTAGY"
     gene            19424..19864
                     /locus_tag="JN749_000287"
     CDS             19424..19864
                     /locus_tag="JN749_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HAY7366822.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="JN749_000288"
     CDS             complement(19851..20594)
                     /gene="ugpQ"
                     /locus_tag="JN749_000288"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAY7366823.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20616..21503)
                     /gene="ugpC"
                     /locus_tag="JN749_000289"
     CDS             complement(20616..21503)
                     /gene="ugpC"
                     /locus_tag="JN749_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAY7366824.1"
                     /translation="MADGEFIVMVGPSGCGKSTLLRMVAGLERVTEGDIWINDQRVTE
                     MEPKDRGIAMVFQNYALYPHMSVEENMAWGLKIRGMGKQQIAERVKEAARILELDGLL
                     KRRPRELSGGQRQRVAMGRAIVRDPAVFLFDEPLSNLDAKLRVQMRLELQQLHRRLKT
                     TSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGTPVEVYEKPASLFVASFIGSPAMNLLT
                     GRMNNEGTHFELDGGIVLPLNGGYRQYAGRKMTLGIRPEHIALSSQAEAGGATGASGA
                     PDGRQHAVAASGGKSAASF"
     gene            complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="JN749_000290"
     CDS             complement(21583..22428)
                     /gene="ugpE"
                     /locus_tag="JN749_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HAY7366825.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="JN749_000291"
     CDS             complement(22425..23312)
                     /gene="ugpA"
                     /locus_tag="JN749_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HAY7366826.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="JN749_000292"
     CDS             complement(23410..24726)
                     /gene="ugpB"
                     /locus_tag="JN749_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HAY7366827.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25123..25836)
                     /gene="livF"
                     /locus_tag="JN749_000293"
     CDS             complement(25123..25836)
                     /gene="livF"
                     /locus_tag="JN749_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HAY7366828.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSSYLGG"
     gene            complement(25838..26605)
                     /gene="livG"
                     /locus_tag="JN749_000294"
     CDS             complement(25838..26605)
                     /gene="livG"
                     /locus_tag="JN749_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HAY7366829.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26602..27879)
                     /locus_tag="JN749_000295"
     CDS             complement(26602..27879)
                     /locus_tag="JN749_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HAY7366830.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27876..28802)
                     /gene="livH"
                     /locus_tag="JN749_000296"
     CDS             complement(27876..28802)
                     /gene="livH"
                     /locus_tag="JN749_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HAY7366831.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28850..29959)
                     /gene="livk"
                     /locus_tag="JN749_000297"
     CDS             complement(28850..29959)
                     /gene="livk"
                     /locus_tag="JN749_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HAY7366832.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30383..30766
                     /gene="panM"
                     /locus_tag="JN749_000298"
     CDS             30383..30766
                     /gene="panM"
                     /locus_tag="JN749_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HAY7366833.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30965..32068)
                     /locus_tag="JN749_000299"
     CDS             complement(30965..32068)
                     /locus_tag="JN749_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HAY7366834.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="JN749_000300"
     CDS             complement(32339..33193)
                     /gene="rpoH"
                     /locus_tag="JN749_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HAY7366835.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="JN749_000301"
     CDS             complement(33438..34496)
                     /gene="ftsX"
                     /locus_tag="JN749_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HAY7366836.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="JN749_000302"
     CDS             complement(34489..35157)
                     /gene="ftsE"
                     /locus_tag="JN749_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAY7366837.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="JN749_000303"
     CDS             complement(35160..36656)
                     /gene="ftsY"
                     /locus_tag="JN749_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAY7366838.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36806..37402
                     /gene="rsmD"
                     /locus_tag="JN749_000304"
     CDS             36806..37402
                     /gene="rsmD"
                     /locus_tag="JN749_000304"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HAY7366839.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37392..37661
                     /locus_tag="JN749_000305"
     CDS             37392..37661
                     /locus_tag="JN749_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HAY7366840.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37664..38023)
                     /locus_tag="JN749_000306"
     CDS             complement(37664..38023)
                     /locus_tag="JN749_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HAY7366841.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38164..38790
                     /locus_tag="JN749_000307"
     CDS             38164..38790
                     /locus_tag="JN749_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HAY7366842.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38864..41062
                     /gene="zntA"
                     /locus_tag="JN749_000308"
     CDS             38864..41062
                     /gene="zntA"
                     /locus_tag="JN749_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HAY7366843.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="JN749_000309"
     CDS             complement(41164..41409)
                     /gene="tusA"
                     /locus_tag="JN749_000309"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HAY7366844.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41630..42295
                     /locus_tag="JN749_000310"
     CDS             41630..42295
                     /locus_tag="JN749_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HAY7366845.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42368..42925
                     /gene="dcrB"
                     /locus_tag="JN749_000311"
     CDS             42368..42925
                     /gene="dcrB"
                     /locus_tag="JN749_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HAY7366846.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42929..44146)
                     /locus_tag="JN749_000312"
                     /pseudo
     CDS             complement(42929..44146)
                     /locus_tag="JN749_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            44278..45327
                     /locus_tag="JN749_000313"
     CDS             44278..45327
                     /locus_tag="JN749_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7366847.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            45382..45969
                     /gene="acpT"
                     /locus_tag="JN749_000314"
     CDS             45382..45969
                     /gene="acpT"
                     /locus_tag="JN749_000314"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HAY7366848.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46080..47654
                     /gene="nikA"
                     /locus_tag="JN749_000315"
     CDS             46080..47654
                     /gene="nikA"
                     /locus_tag="JN749_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HAY7366849.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47654..48598
                     /gene="nikB"
                     /locus_tag="JN749_000316"
     CDS             47654..48598
                     /gene="nikB"
                     /locus_tag="JN749_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HAY7366850.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48595..49428
                     /gene="nikC"
                     /locus_tag="JN749_000317"
     CDS             48595..49428
                     /gene="nikC"
                     /locus_tag="JN749_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HAY7366851.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLILGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49428..50192
                     /gene="nikD"
                     /locus_tag="JN749_000318"
     CDS             49428..50192
                     /gene="nikD"
                     /locus_tag="JN749_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HAY7366852.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50189..50995
                     /gene="nikE"
                     /locus_tag="JN749_000319"
     CDS             50189..50995
                     /gene="nikE"
                     /locus_tag="JN749_000319"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HAY7366853.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51001..51402
                     /gene="nikR"
                     /locus_tag="JN749_000320"
     CDS             51001..51402
                     /gene="nikR"
                     /locus_tag="JN749_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HAY7366854.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51522..51881)
                     /locus_tag="JN749_000321"
     CDS             complement(51522..51881)
                     /locus_tag="JN749_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HAY7366855.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51878..52153)
                     /locus_tag="JN749_000322"
     CDS             complement(51878..52153)
                     /locus_tag="JN749_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAY7366856.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(52212..53336)
                     /locus_tag="JN749_000323"
     CDS             complement(52212..53336)
                     /locus_tag="JN749_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HAY7366857.1"
                     /translation="MRQLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="JN749_000324"
     CDS             complement(53336..56071)
                     /gene="rbbA"
                     /locus_tag="JN749_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HAY7366858.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56068..57135)
                     /locus_tag="JN749_000325"
     CDS             complement(56068..57135)
                     /locus_tag="JN749_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7366859.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57501..59123)
                     /locus_tag="JN749_000326"
     CDS             complement(57501..59123)
                     /locus_tag="JN749_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7366860.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINKNVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59385..60974)
                     /locus_tag="JN749_000327"
     CDS             complement(59385..60974)
                     /locus_tag="JN749_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HAY7366861.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEESQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61470..62389
                     /locus_tag="JN749_000328"
                     /pseudo
     CDS             61470..62389
                     /locus_tag="JN749_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908812.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
     gene            complement(62476..62969)
                     /locus_tag="JN749_000329"
                     /pseudo
     CDS             complement(62476..62969)
                     /locus_tag="JN749_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(62923..63687)
                     /locus_tag="JN749_000330"
     CDS             complement(62923..63687)
                     /locus_tag="JN749_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7366862.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSANLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNGETYAICSEVLLYD"
     gene            63934..64695
                     /locus_tag="JN749_000331"
     CDS             63934..64695
                     /locus_tag="JN749_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7366863.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSLQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64802..65314)
                     /locus_tag="JN749_000332"
     CDS             complement(64802..65314)
                     /locus_tag="JN749_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366864.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(65453..>66599)
                     /locus_tag="JN749_000333"
     CDS             complement(65453..>66599)
                     /locus_tag="JN749_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7366865.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDQK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPVPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16175 a        17873 c        16342 g        16209 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgtaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaagtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgccgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgagg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta attcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagtag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatgtcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgaga ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg
    17881 ggctaccggt aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg
    17941 acagcgggcg tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt
    18001 tcggtacgcc cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct
    18061 cgcccgcgac aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt
    18121 taccgtcttt atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct
    18181 tgccagggcg aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg
    18241 atttggcata ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc
    18301 caagatattc cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg
    18361 catcggcgct gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt
    18421 gtacgatatg gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat
    18481 aatcgccgct tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga
    18541 tcaagccacc gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga
    18601 attcgtccgg gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca
    18661 gcgtgccgcc ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt
    18721 gattcggcaa cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga
    18781 taaaaccatc gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc
    18841 cgtatttatc cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat
    18901 gcgaagtgag tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg
    18961 tcgctttggc gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc
    19021 ggattaacat aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg
    19081 cgtagcccac cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc
    19141 ccacctgagt ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat
    19201 ggaagacatc ttcctccaca ccatacgaga cgggcggcgc taaaacaact tcctgagagc
    19261 agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga
    19321 ttaaggggca accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg
    19381 gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat
    19441 tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc
    19501 taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca
    19561 gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca
    19621 gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg
    19681 ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag
    19741 caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga ggagaaacgg
    19801 cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc
    19861 gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc
    19921 agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg
    19981 tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg
    20041 cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca
    20101 cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac
    20161 agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc
    20221 gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg
    20281 cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg
    20341 taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc
    20401 cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc
    20461 gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc
    20521 gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa
    20581 ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg
    20641 ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca
    20701 ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag ggtcatttta
    20761 cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc gtccagttcg
    20821 aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg gctgccgata
    20881 aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt gccaatctgt
    20941 tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc ctcaacctga
    21001 tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg cagttcaaga
    21061 cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa caagaacacc
    21121 gccggatcac gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc gccggaaagc
    21181 tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc ttctttaacg
    21241 cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc catgttttct
    21301 tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat cccgcgatct
    21361 ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc ggtcaccctc
    21421 tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg cccgaccatc
    21481 acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta ggttttgcca
    21541 tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct cactatcgac
    21601 caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg ggatcagggt
    21661 taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag tggcgatcat
    21721 ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca acaacggcca
    21781 caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca gattagtttt
    21841 ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt cgatccgcgc
    21901 cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa acgtggcggt
    21961 cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct tcaggttggc
    22021 gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg tgataaaaat
    22081 catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa atgccgagag
    22141 catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc tgttaagcaa
    22201 catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt tgtggatgtt
    22261 ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg cctgtttatc
    22321 cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga tcccgaggat
    22381 cagcatggta tggctgaata tcgtcagcca cggacggttc tcaattattg gtaacgcacc
    22441 ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa catcaacacc
    22501 accgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg gtagatctta
    22561 taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc gatcaccggg
    22621 aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact caccggggcg
    22681 ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc gatggctgcg
    22741 gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag aaagttgtag
    22801 ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc gctattttgc
    22861 gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc gcgaccgggg
    22921 ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata cggcagcaac
    22981 attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc cgcaaagaac
    23041 agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt cgtccagaag
    23101 gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc gacaaactgg
    23161 ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc gcccgcaggc
    23221 cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata aggcagccag
    23281 cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac tgtgccggat
    23341 gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac gcatccggca
    23401 ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt tcccacgctc
    23461 aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc tctcaagctc
    23521 ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt tggtgaacgg
    23581 caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt ttttctcgta
    23641 aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat aaccggtttt
    23701 ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact tcgccacgcc
    23761 ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc cgataatggc
    23821 gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc cgtagttaaa
    23881 tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca tcgcgcaatc
    23941 accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga agtcgccctt
    24001 cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt tgaactccag
    24061 caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac cgttccaggc
    24121 gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc acttaatacc
    24181 ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg gcggctgttc
    24241 cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg gagtcgagct
    24301 gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac cggaaaccgt
    24361 cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt catacaccgg
    24421 tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct gcaaaatagc
    24481 cggagcgttg ccggtacgaa atgcggcaat ccccgcgctt aaattctgtt cgtagttgcc
    24541 tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt gggccagaga
    24601 atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg tcgtcactgc
    24661 ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat aatgtaacgg
    24721 tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt tatgctcgcg
    24781 gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt tgtaagatag
    24841 taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat gggagatggg
    24901 gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac tcatcgtttc
    24961 atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat tcaatatatt
    25021 gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca tttgtcacct
    25081 gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc ctaaatacga
    25141 acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca ccacatggcc
    25201 gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt tctgctcgac
    25261 gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa tttgctggat
    25321 aataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg gattgctcat
    25381 cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc ccgcccgctg
    25441 aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc gctcctgaaa
    25501 ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg tcatccgcga
    25561 gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt tcgccgtctg
    25621 ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc gcggatcgcc
    25681 gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga gagtgacaat
    25741 ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc cgtagtgggc
    25801 gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct aaataggcac
    25861 ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt ggcgtcccct
    25921 gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg tgttcaatca
    25981 acaagatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc agctctttgg
    26041 tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc ggctgcgtca
    26101 ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta cttgcctgac
    26161 ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg tcgagcgctt
    26221 cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc ccggttttca
    26281 gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg aacagacgca
    26341 catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt aaaccttcaa
    26401 gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg gtcaggcagt
    26461 taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt tcctgcgggt
    26521 acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc atcagaccgt
    26581 taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt ttttcagctt
    26641 cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca tcagcaccat
    26701 caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt cgcgcgacac
    26761 caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc cgagcaccac
    26821 tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga caaagccctg
    26881 acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg taaaagcggt
    26941 cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt cttcacgcaa
    27001 cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa acaggcttaa
    27061 caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg gatcgtattt
    27121 caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac ggctgaactc
    27181 gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc cggtgatttc
    27241 agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa cgatcgccag
    27301 atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg ccgctgccat
    27361 cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat tgagcagcgc
    27421 aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca gaccagaaag
    27481 accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca gagtggcgat
    27541 atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca gcagcgccac
    27601 gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa acttcggccc
    27661 ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga aaaagaccac
    27721 cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga ccaccagttt
    27781 ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa agaacatcgc
    27841 ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac ctccgggcga
    27901 cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa tgagaccaca
    27961 tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc taggatcagc
    28021 ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc ggtaaaggct
    28081 ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg accgagcagt
    28141 acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat cacccggtcg
    28201 gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg acaggcgcgc
    28261 cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa ggtgacaatc
    28321 cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg gcccaccacc
    28381 cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt caggctgacg
    28441 tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag gcgcttagag
    28501 ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct ggcgatgacg
    28561 attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc catcatcatc
    28621 agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc gccgtgggcg
    28681 aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat cagcgcgtag
    28741 gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa ctgctcagac
    28801 ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat cacttggctg
    28861 ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc ttaagatcgc
    28921 ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct tttaaatctt
    28981 tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc agagattgca
    29041 ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct gctttcagcg
    29101 catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata gtgaccaaca
    29161 tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca ccttctggcc
    29221 ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc cccatttccg
    29281 ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg gcgatcagcg
    29341 cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg gcgttagccg
    29401 ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt ttgtcgtgaa
    29461 tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc gtaggcccct
    29521 gggaagagtc cagtccggca gtacgcataa tgtgttgata accgcgttgg gtcagctccg
    29581 ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata tccgacgctg
    29641 gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg ttaacgattt
    29701 tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc acgccaacca
    29761 gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga cgcgcgccgt
    29821 taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca acggcgactt
    29881 taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct gcgatgatag
    29941 ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt gctttgtttt
    30001 tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc ctgtctgatt
    30061 aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt tcagtcgatt
    30121 aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc ctttgtcagc
    30181 ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt atgctggaca
    30241 tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt aatcacctgc
    30301 caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact ggttccactt
    30361 ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa actttagcga
    30421 ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct ctttacaggt
    30481 tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg cggccgtgcg
    30541 ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg aagtcacccg
    30601 ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc ctggcgtttc
    30661 atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg cgtttatgca
    30721 ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt caagttgatt
    30781 tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg cctataatgt
    30841 gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt tacgccgcat
    30901 ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag ttgccgatta
    30961 acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg ccgaactcaa
    31021 agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta tccacggagt
    31081 tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg cccgcctgca
    31141 aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc tgttttttcg
    31201 ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc ttcggcttgg
    31261 tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg ttagccacac
    31321 cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc aggatttgcc
    31381 ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt ttcagacgcg
    31441 ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac accacgttag
    31501 cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg tattgctgtt
    31561 tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat ttcgccgccg
    31621 tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag ccacgggcgg
    31681 tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct tcgtagatgt
    31741 cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta ataccgtcgt
    31801 taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg tcatatttta
    31861 cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca accgcctgct
    31921 ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt gcacccacga
    31981 ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg atacatcctg
    32041 ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg gttattacgt
    32101 gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac ccgtgcttta
    32161 gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct attttgacag
    32221 tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga ttattgtaga
    32281 ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat agcggaaatt
    32341 acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg cgcacacgtt
    32401 cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg tcttcgtcca
    32461 gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc gcgtcggtca
    32521 gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca aagttagatg
    32581 atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg tcgtcggaag
    32641 acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt acgtctttgc
    32701 tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac cagcccagac
    32761 gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg gtcgcaactt
    32821 tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc cagtggacgg
    32881 cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct ttcatcaggc
    32941 cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca taattacgag
    33001 caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct tccagatcgc
    33061 catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac atcggccacg
    33121 cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct aaactttgca
    33181 ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt ggtagcaaca
    33241 actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag accgtgattt
    33301 tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga catagagatg
    33361 aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt gcagggaaag
    33421 agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac cgtggcaagc
    33481 cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca ttcatcgaac
    33541 gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc caccgctgac
    33601 gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc agaaaatccc
    33661 agcagcgcac caccgtacag gaacgggcga aggatgaaac catccgtcgc accaatcagt
    33721 ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct gttgccgatg
    33781 acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg cccaaccagc
    33841 ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc gtcaatgccg
    33901 ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg aaaatcgagt
    33961 ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc cagcgcacca
    34021 ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag atagttcact
    34081 ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc gtcatcgtcc
    34141 agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt cgccgcctga
    34201 ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat ggcgataacc
    34261 atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc gccgtggaag
    34321 gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt tggcgaggat
    34381 ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga tttacggaag
    34441 cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt attcatggcc
    34501 cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac gccgtgaaat
    34561 caggttaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa attcttcaaa
    34621 cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt cgtccgccag
    34681 cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt gttcaccgcc
    34741 ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat ccagtgccgc
    34801 cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg ggatcgccac
    34861 gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca tgccaatctg
    34921 gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc cgctaaacca
    34981 gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg tacttttccc
    35041 tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga acgtaacgcc
    35101 ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc gaatcattgt
    35161 taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg acgcaaatcc
    35221 tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc agccaccgag
    35281 aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc ggttaagcca
    35341 acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct ggcatcaata
    35401 gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg gacgattttc
    35461 ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc ggcaatcagg
    35521 acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga ggcggaatcc
    35581 gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac ctgaagctgt
    35641 tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt accctgctgc
    35701 tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt gacgcccacc
    35761 atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac tttcgccaga
    35821 atctcaccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg aagctgcttg
    35881 cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac gcccacatcg
    35941 gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt tttaccgcgg
    36001 aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct gcgtttcagg
    36061 cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc ttcaaccggc
    36121 tgttcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc ggcagccagc
    36181 gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc ttctgccgct
    36241 tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctggcgaaac ctcttcggcg
    36301 ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc cggcgtttct
    36361 tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt ttcactttca
    36421 acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc ggcttcagta
    36481 tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc ttgcgcctga
    36541 acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc cggggtctgc
    36601 tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt cgccatttgc
    36661 gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca tggcagctaa
    36721 aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc aagtatatgt
    36781 tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca gcggccaaat
    36841 ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata gcccaggtct
    36901 gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc cggtcattgt
    36961 tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg aagcgttatc
    37021 gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc agcagttaat
    37081 taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca acgcaatgtc
    37141 attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac cgttccgccg
    37201 tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg ctgacgaagc
    37261 cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg caaactggtc
    37321 attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac acgaagcaca
    37381 aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt tggggatttt
    37441 taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt aacgtggcgc
    37501 tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc actttaccga
    37561 aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc ggcgtgtttg
    37621 aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt tcgccaacaa
    37681 agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccacg gtcagggcgt
    37741 ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc ggtttgaagc
    37801 ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc gaacggcgat
    37861 cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg agcggagcca
    37921 tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg gcgacgacaa
    37981 ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc cctcatcgga
    38041 aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca ccctcgccac
    38101 taaactggaa gcaagaccgt aggcattccg cttacgaaaa aataacgaat tcaaggaact
    38161 aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt ggatgcatcg
    38221 tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct tctcctgctg
    38281 ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct ggcgggactg
    38341 tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct gatgtacgcc
    38401 atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag tcagatgacg
    38461 ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct gctactggcg
    38521 attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat cggcatgacg
    38581 ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc tccggcgctc
    38641 tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct ggggagccac
    38701 tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt tgccggtcac
    38761 ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc gactccagag
    38821 tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga cacctgacaa
    38881 tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg tacagaacgc
    38941 caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg aaaacgtctc
    39001 cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg cgcgcaaggt
    39061 agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt tcgccaccga
    39121 aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg cggtgcaaaa
    39181 agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat cgcgcctgaa
    39241 agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg gtctggagca
    39301 gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg ggctgtaccc
    39361 gattgctcat caggcattac ggctgatcaa atccggcagc tacttcgcca ttgaaacctt
    39421 aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag ctgcgatggt
    39481 gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc gcgcgcgtca
    39541 gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc gtaacggtga
    39601 gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag tcgccgcagg
    39661 tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg atgaaagcgc
    39721 cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc ctgcaggagc
    39781 caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg ccagcgccat
    39841 tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca ttgagcggtt
    39901 tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc tgctggtaac
    39961 gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata aaggtctgac
    40021 gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg cgataacctc
    40081 cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg cgctggaaca
    40141 actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg tcggtaaacc
    40201 gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc tgacactggc
    40261 ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac gcgaagcaca
    40321 ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg ggtctggcat
    40381 tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac atcccgctga
    40441 tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg tgctggtagt
    40501 acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg ccgacgctac
    40561 aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca ccggcgataa
    40621 tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag cgggcctatt
    40681 gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc tggcgatggt
    40741 cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga ttgctatggg
    40801 tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata accacctgcg
    40861 cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc gccagaacat
    40921 caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag ggatgaccgg
    40981 gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg cgaatgcgtt
    41041 aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga tcggtcccct
    41101 cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc caatcagccc
    41161 ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct ctttcgcaac
    41221 cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg ccggatcgtc
    41281 ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc gcaccatcat
    41341 caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc tggaaaagag
    41401 atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc tgtgagtccg
    41461 taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa cgtaaagcag
    41521 tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta acagacgtgt
    41581 cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata tgaacgtttt
    41641 ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc ttctggtgat
    41701 cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc ataccacctg
    41761 gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc gtatttttgg
    41821 cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat taatctccta
    41881 cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac tcgcccactt
    41941 caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc tggggcaaat
    42001 cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc tggcaccgac
    42061 agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca ttgccttctg
    42121 gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg tcgattactg
    42181 tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat tactcaatat
    42241 gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga gttaaaggtt
    42301 cgtaatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta tcgaaaggaa
    42361 gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta tggggcttgc
    42421 ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggtcgcgg aaagtaacgc
    42481 taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc cagcggatat
    42541 gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct ggtccgacgc
    42601 caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag atctggcggt
    42661 gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc aagtggtaac
    42721 caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta ttatctccgc
    42781 gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc aactgctgac
    42841 catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg cagaaaacat
    42901 cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca gggacgtgtt
    42961 ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc agtcccgccg
    43021 ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc agtggtccag
    43081 tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc ccctaatttt
    43141 gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc gaaaacccgg
    43201 cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg ccaaccagta
    43261 gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca ccgatacggt
    43321 taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac agcgtcagcg
    43381 cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag gtggcgatga
    43441 cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg cgcccaagca
    43501 ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc gggatcgcca
    43561 acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc aagccgcccc
    43621 agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa gtgacaatgc
    43681 cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg ccccataggg
    43741 tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc aggcaaagta
    43801 ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac cccagaccgc
    43861 tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc aaatcggcgt
    43921 aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg ataaccaatc
    43981 ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat acagcgagcg
    44041 gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca atgcgcaaat
    44101 tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg atcaccacat
    44161 ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag taagaatcag
    44221 aatattgctg gcgtgactcc cgctacactt aacacaaaaa gtcacaagga agccccaatg
    44281 gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc ctcgctggta
    44341 gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt actggcgctg
    44401 ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg agtacaacgc
    44461 cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc gctggtcggc
    44521 gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa taaggagctg
    44581 acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca tatgtcgccg
    44641 gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac agcgctaatg
    44701 accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt agtttttatg
    44761 ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa cccacagatt
    44821 cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc gttaaagacg
    44881 ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat gggcgtacag
    44941 tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa tatcggcgcg
    45001 gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt ttacgaatgt
    45061 attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat tttagaacca
    45121 cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc attgttaatc
    45181 tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac cagcgtgtgt
    45241 aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact ggggccggac
    45301 agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca ctttcatttt
    45361 ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc gaccttaagc
    45421 gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg acgcgaacgc
    45481 tggctggcgg ggcgtgcatt gctttcgcac acgctttccc cgctaccgga gatcatctat
    45541 ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa cttaagccat
    45601 agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg cgatatcgaa
    45661 gtgattcgcc cgcgcgccaa ctggcgctgg ctggcgaacg ccgtgttcag cctcggggaa
    45721 cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg gcgcatctgg
    45781 acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat cgtcagcgta
    45841 gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa tttaagcctt
    45901 gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg gatcgattca
    45961 gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta atcagtatga
    46021 cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt ggggttacca
    46081 tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct tttatcgtcc
    46141 atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg ccactaaacc
    46201 cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa ccattggtga
    46261 aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact cattcagaag
    46321 atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac ggtgaaccgt
    46381 tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt caacgtcacg
    46441 cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa acagagctgc
    46501 aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg ccccgtcctt
    46561 tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga attaaagcgc
    46621 cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac gatgtcttcg
    46681 tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc tttaacgtca
    46741 tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac ctgctgtacg
    46801 gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat ccggcttacc
    46861 acacccaact gtcacagccg ctcgaaaccg tgatgctggc gctcaatacc gccaaagccc
    46921 ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa aaatcgctga
    46981 ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc ccttctgtgc
    47041 cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg aaagagttgc
    47101 tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa aatggtcagc
    47161 cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg atggcggaaa
    47221 tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc gaagaagaga
    47281 gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac cgcacctggg
    47341 gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg cacgctgact
    47401 tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc ggcgaagtgc
    47461 tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg acccgtctgc
    47521 atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta tcaaaaccgg
    47581 agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa cagattaaac
    47641 cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga tcccgatggt
    47701 gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg acccggcgct
    47761 cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct ctacccgcac
    47821 catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt ggaaggcgct
    47881 gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg atatgctgaa
    47941 cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc tgctcacttc
    48001 cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt tcgccgtgcg
    48061 ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt tgctagtgat
    48121 ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct ggcagcacat
    48181 cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc gactgctgcg
    48241 cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc tacgcggcct
    48301 gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc cgatgatcac
    48361 cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg aaaacatctt
    48421 tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg actatccggt
    48481 gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt tgattgtcga
    48541 tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc acgcgtgaac
    48601 tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc cctgctggcg
    48661 ctgattgcgc tcaccagcca gtggtggctg ccgtacgatc cacaggcaat tgatttgccg
    48721 tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt aggtcgcgat
    48781 attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat ggcctgcctg
    48841 ctgctggtgc tgatattagg gctggttatt ggcggcagcg ccgggttgat tggcgggcgc
    48901 gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac ctcgattctg
    48961 tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat cgccatcgcc
    49021 ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc actacgccaa
    49081 cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt gtttgtcgat
    49141 catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga tatcggccat
    49201 atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc gccgaccgcc
    49261 gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc gctgcaaatg
    49321 ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt gggtgacgca
    49381 ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg ccgcaacaga
    49441 ttgaactacg taatatcgcg ctacaggccg cgcagccgct ggtacacggt gtatcgttaa
    49501 ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg aaatcattaa
    49561 cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg ggggaaattt
    49621 tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt gccaccatca
    49681 tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac gcgcgggaaa
    49741 cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc atagaagcgg
    49801 tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg agcggcggca
    49861 tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt atcatcgccg
    49921 atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat ctgctggaaa
    49981 gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg ggcgttgtgg
    50041 cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa cagggcgatg
    50101 tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt tccgctcatc
    50161 tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg gcctttccca
    50221 tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc tgaataacgt
    50281 ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg gctgcggaaa
    50341 aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga atattagctg
    50401 gcgcggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc gccgtgatat
    50461 tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg tgcgcgagat
    50521 cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac tggcgcgcgc
    50581 cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac gcccaccgca
    50641 gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg tcgaaccgaa
    50701 attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg cgggcgtcat
    50761 tcgcctgctg aaaaatctac aacaacagtt tggcactgcc tgcctgttca tcactcatga
    50821 cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg gacaaatcgc
    50881 cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac gtgtgctaca
    50941 aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg tttaacgcca
    51001 atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga cagcctgagc
    51061 cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg tagtgccctg
    51121 gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc gtatgtgtat
    51181 gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca tcaccacgac
    51241 ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga aatcgccgtg
    51301 ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc ccagcgcggc
    51361 gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc taaaacaagg
    51421 aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca tagatagatc
    51481 ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg attcaaacgc
    51541 tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc agccgcatta
    51601 ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat cttttctgta
    51661 cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg cagggaaagc
    51721 tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata acctgacaag
    51781 cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac agctggttgc
    51841 ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc cctatactta
    51901 gtaaccagtc acgcacgctt tctacagcgc ctttatcggt atctggcgac ggatgaggcc
    51961 gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct cttccttcct
    52021 taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg atcccttgag
    52081 gaacaggtgt tttaaatatt tgatccaatg tatttttttg tttcttccgt aaagcctgta
    52141 ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt atgaagtcaa
    52201 gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag tgcgatggtg
    52261 aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat ttcgaatccg
    52321 gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg catggtcagc
    52381 ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc ggaaagcatt
    52441 tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga acgcgctatc
    52501 gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac gcccagcata
    52561 aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat cagcaccagc
    52621 gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc catcatgatc
    52681 tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc acgctcgcgg
    52741 atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat gatcgccatc
    52801 accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt ttccagcgat
    52861 accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc accgttgata
    52921 atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc atcgacgttc
    52981 acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg cggaatattt
    53041 atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc ggtgatcatc
    53101 tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga taactgcgat
    53161 tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt aacggtcgct
    53221 gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc tttatcaccg
    53281 agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaactg gcgcatcatc
    53341 cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg gctatcagca
    53401 acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac gttccgcggg
    53461 cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca ggcccttcca
    53521 gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat gtgataatcg
    53581 ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc agccccattc
    53641 cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa ctgcctttat
    53701 gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc agcatcccca
    53761 gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg ggggtcacgt
    53821 aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg cttagcattg
    53881 acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc aggcttttta
    53941 cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg ctggcgggtg
    54001 tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc acgtaacctt
    54061 ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt tccacaggcg
    54121 taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc gtgatatcgc
    54181 ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt tcgataaagt
    54241 aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg tcgcggtcga
    54301 gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt atcagcatca
    54361 ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc agttccagcg
    54421 cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc ggcgcgtggg
    54481 tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc gcttcctgca
    54541 aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc agttcctgcg
    54601 gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg caacgttccg
    54661 cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc gagagatcga
    54721 ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta ggctcatcga
    54781 ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc tggcgaatgc
    54841 cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa cgctcgctca
    54901 tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg gcatgtaact
    54961 ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac atatagccca
    55021 cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc cacgcctcac
    55081 cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta ccgcagccgt
    55141 tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg tgatcaacgg
    55201 caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc tctgcgtttt
    55261 caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct tgcggtaaca
    55321 gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc tcttcggcgc
    55381 tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag cgttcggcct
    55441 cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg cgaatactgt
    55501 cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt ggctcatcaa
    55561 ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt tgcttcatcc
    55621 cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg ctggtcagca
    55681 gctcattaat tcgcacttcc cgctccgctt tatcgtgacc gaacaggcga gcgaaaaaat
    55741 cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc tgcggcatcc
    55801 aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg ccacccagca
    55861 ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat aaactcgact
    55921 tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata tcgagagtga
    55981 tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt tgcgcgacag
    56041 gcgggacagg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga ggtcgtcagg
    56101 ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg ttttgacata
    56161 ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga acatcagttt
    56221 cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac tggcgacaaa
    56281 actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc gggcttcacc
    56341 gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat agacgtcgct
    56401 gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg gctcggcaac
    56461 ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga tatctgcggc
    56521 aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa tgatattggt
    56581 gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg attccagcgc
    56641 agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag aaatagcccc
    56701 tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca gttcggcctg
    56761 gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct ccagcaaagc
    56821 ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt ccagtcgctg
    56881 ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa caaactggcc
    56941 ttctttcacc agaatggtgt cgatacgccc ggcagttttg ctggcgatat ccacttccgt
    57001 cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg ggcgcaacag
    57061 ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg ccagatggcg
    57121 cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg cgtgttacaa
    57181 ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa acgacaaata
    57241 cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagcgaaaac gggtagtaat
    57301 gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa gtgtgatcta
    57361 taaaactggc tgacaaaacg gtatccatcc tgttggatcg cgtttcacgc ctgttcagtt
    57421 gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat agtaacatta
    57481 atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat caaaggcata
    57541 agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat ctctggccct
    57601 gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg tttactgtca
    57661 cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc accatttccg
    57721 ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag tgcttcaagt
    57781 ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca agagatagcc
    57841 cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt aataatttcc
    57901 gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt tatcggtcct
    57961 ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc cattattttg
    58021 tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt atcctcatca
    58081 agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt ttcatgattt
    58141 ccagctaata catgaacatt tttattgata ctcattatgc gttttaacaa atcgatgata
    58201 aattgaacgc cacagatatt actaaatcga tcgccggttt ggtcgccaag aaacgtacat
    58261 ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa aacattatct
    58321 cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc attaaagttc
    58381 tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct aaggaaattt
    58441 tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa aactgcaccg
    58501 actgagctat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc aacaggtgaa
    58561 caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc gaatttaata
    58621 tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga ctcagtaact
    58681 gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac aatgctgcac
    58741 caattaatat cggtattatc aagagggcaa ccgtgatttt tactattaat tgtcacaata
    58801 aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag catactatat
    58861 ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat agattgttgg
    58921 aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat tgtcctggca
    58981 atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc tttagtgaag
    59041 ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact gccattagtg
    59101 cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag gaatacatag
    59161 ttaacatttt atcttatgac atacacctca tattcgatgg gaaaataatt atattttgca
    59221 caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag agaaagtccc
    59281 taaagattgt agaggcgtca tcagtacata cctctacaat cgattaagaa aacctatgaa
    59341 atactggcaa aattaaaaag agataccaca gcactcatga agttttattg aggttgaaaa
    59401 caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat cttccaagaa
    59461 tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa tccataatgg
    59521 ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc ttcaggtttt
    59581 ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg tgctatagca
    59641 ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt tttattaaat
    59701 atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg aacctgcaaa
    59761 gcaccgagac aataacattg actttcttca tttcttatta ttccgtgatg actggttaaa
    59821 actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt atacgtatca
    59881 ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa attgatttca
    59941 tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt accctccata
    60001 tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt actaaatcta
    60061 tcgcctgtat gatccccaag ggggatacaa ggggttatcg catcgtcctt taaaaaaaca
    60121 tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta gagcaattta
    60181 ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa ttcacataat
    60241 aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga ataaagtaca
    60301 gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg tgggttaatc
    60361 acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata gctatcaaac
    60421 ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt aaaattaccg
    60481 ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt ataaattatg
    60541 tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc aatcttaact
    60601 gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt aaagataaac
    60661 gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga tggctcacta
    60721 ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca gtgaccatgt
    60781 tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc attatccagt
    60841 ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg gtgttgatga
    60901 ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt aaatatattg
    60961 tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa ataaatgata
    61021 catgtctgaa agacatattt acgacgatat aaaacaataa tgcttatatg ttcgctgatt
    61081 ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca tccctggaag
    61141 acaattgtaa aaaaaacaac agaataagaa ttaatcttag gataaatatt tatttatcat
    61201 ggccttttga ccgctaaaaa atggcactcg gcacagtaaa ccccgctata gttatgctat
    61261 acatttgtct ttaatgatat ctcgtcgtta tattaacagg ctgaaattat cattaatgca
    61321 catttcaata ttagcaggga tacctgagga gtttaaagtg aacatatata tcgggtagct
    61381 tttcaaatta atccctttga ttataggctt aatttgcatc gcgttaggtg gctttgtgct
    61441 ggaaagttca gggcaacgtc acgggtcatg tgctgatttc tctggcggcc atatgcctgg
    61501 cattattcac taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt
    61561 acaatacatt gttccccatt attggctatg cggggtcaat tatcaccatg atatggggtt
    61621 gggcactgtt agcaggtaat gatgtgatgg cagacgagtt tgttgccggc catgttattt
    61681 ttggcgttgg tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc
    61741 tgctcattcc caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt
    61801 cttcattaat cggtaattgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca
    61861 tttggtctat tacgctgtta catagtgccg acataactcc gcattatgtc gccttgggtt
    61921 aaccgcaatc tgtgcctgtc taattggcct agtggccaca atcgtccatc aaacacgtaa
    61981 tacgttctca tccaaagagc actggctgtg gtgttattgg gttatttttc tcggctcagt
    62041 cacggtgctg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    62101 cggcattatt ctcatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    62161 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgataccggt
    62221 atttacctgt ctgttttgct tgtttttgtc atcattcctc gcggaaatcg cacaaactga
    62281 gctggactac tttattccat ctcgggttct ggttggatta agcgcagtat gttttacatt
    62341 gttctctatt gtttcaatat tagaggccgg ttctgcgaaa aaaaattaaa atttacataa
    62401 tcccagcgtt attcatatct taacgctggt tttttacgaa cttagtgaaa taatgtagaa
    62461 tataaaatta taattttata ttggagcaca tgtgttaatg atcaaccccg ggatactaaa
    62521 aatatagaca ttattaaact tgcttagcct cgcattgcct ttacctgtta aagatgcatc
    62581 gagaaaattg agtctgaagt tctcatcctt tatctgattt tccagtcccg aaactttggc
    62641 cttagcatta cctgagatcc gtccgttact atctattttg taggtaaaaa aatacgaatg
    62701 ggaaaatttc ctcggtgtat cttcatcagt taagccaaca atcgtaaaac taccgctacc
    62761 atcttttttt aaaatgactg aaaggttagc agagtagtat aaagaatgat ctgttttagc
    62821 atttcttgtt aacgtcgacg tacattcaag agacgttgtg ctaccatcac gagccttcca
    62881 gagagcaaaa aaacacacct gtagcaaaaa tacattttct tattagtcat ataatagcac
    62941 ctctgaacaa attgcataag tttcgccatt agaggatttt atacatcttg ataaataccg
    63001 ctgcccttcc ccaagcaaat aatgcatact atttacatca tatataaaat ttgcacctgg
    63061 cacacagggt aaatgctttt tcgaaatttc agcggcctct atttgagatt tagcgaggcc
    63121 aatctcgtta agattcgctg aaagatataa atcacaggtg tcaatcttac ctgatttaac
    63181 caatggataa tccgttatac tgtaggtatc tttaaaagcc acggcaaaca tttcaataat
    63241 aacaattact accacaagcg aaatcaaaat gcgaaaaaga ggaatactcc ttttgcgctc
    63301 tactttttca acaacatctt cttgtaaatg agaatcatca taagattgtt tacttattgt
    63361 tatgttttca ctgatgtaaa agcctacttt gggtatcgta agaatcacat tttcttcaac
    63421 accaatattt ttaaaacttt tgcgtaacag ggatatatat tggttcaatg tgttgtttga
    63481 cagatcaaat gaaaatctct ccaaaacgct atcaatgatt gagttcctgt caaccgctcc
    63541 accattactt tccagtaaca ctgccaaaat acaccccgcc gtagctggca aatttatctc
    63601 atcctgggta tttacattcg agagcgagca tttgttcgga tcaaaaataa tcgcgtcatt
    63661 gataatgaaa aaacatctcc gctccatgca ggcaacaaca caaaataaga ttttgtcatt
    63721 ttagcctatt cctggtggaa ctgacacaag aaactaaaat acaaatataa ccctgaaaaa
    63781 gtcatataaa acttatatcc acagaataaa aaaacaaaac atgtcagttt tatcaatata
    63841 aagtatcgca aggcgaaaag tgatgcaaga atactcctaa gtaaatgaaa gcgcaccaca
    63901 tgaaattatc aacacatgta atttcatcat taggtgctat cgaaaataac tcaggagcta
    63961 cttaagatga ataatgtctt actcatgaga cccgatgact cagaatttat gatacctgat
    64021 gactggtcag ggctaaacgc aaaaggcatt acatacgaac aagataatag agtgacagag
    64081 tcttttctgc cttttaaatc tttttattgt tcaccggtgt gcacgacagt tgaactcatc
    64141 tcacaaaata attgttttgc tatgggtatc cgctaccttc tggaaaagaa aagagactta
    64201 aggctaaatc actttaaagc cttctctcat aaatgcgaga caaaacctga cgtgttaatt
    64261 atgaccgggt gcaccgtcgc tgatacttat agtatattct cacgcattgt tgaagaaaat
    64321 ataatgaaaa cgatgacacc tatcgtcatc attactgggc atcactcaga agatctttgt
    64381 tctctggcat cgaaactggg aggagttaaa ataatatcac cgacagaaaa gattgcaaaa
    64441 atagaaaacg ctatcaatga tgcttttaac agcaagccta aaaaaataag atttttaaca
    64501 gatatactct ctcttcaaca aattgagacc atgaaaatgg ttgcgcaggg tagatccgtt
    64561 aatgagattt cagccgcaat gaatattagc atcaagaccg tgtatgcaca taaagttcgt
    64621 gctatcaata agctgggaat taagaaaaaa gtttatgaag cgctttttta taaagcgcta
    64681 attcagttca actgatgtaa aaatcaccct cgcttaataa ataatatatt ctatgtcttt
    64741 ttttataagt taaaaaaggc gggatatcaa atcccgccat gaaattaact aatcatctgc
    64801 attattctgc gtagatataa gcctgggtac ggatggtgta agagtctggc tcaacaacct
    64861 gttctttcat atgggaaacg acgcgatagg caccacctgt tccagtattt ttcagcatca
    64921 tataacgctt accgccaatc tcttcggagg cagctgaagg aggatcctgc tcaagcgttg
    64981 tcagggaaac tttcagttta tggtccgggt tattgtcacc agaaattact gcatccgctg
    65041 aagtactgct ctccgcataa atatcatccg ggaaagttac agcatagtcg tggctctgcc
    65101 catcagatgc gccaacaata cctgttgcca aaggcgtcca tgcagttaac gtaccgctat
    65161 ataccccttt cactggttga atggtattat taacaacgat aggcttggtc aaagtaagat
    65221 tagctgacgc ttcagtaccc tggcttacta aagttcccgc ctgagaagca aaagctgtcc
    65281 ccgccagcat gattgctaac aatgttttct tcattatatt ttctccatga ttttcattta
    65341 cactgcaaaa tgcagtttct ttctttgcat ttacgtgcta tcgcgaaaat gcttccacca
    65401 aataatggaa acatcttcac aaaaataaat tatgagtttc ttaatttaga aactataaac
    65461 atgtcgccgc tatctggtat aaccctgatg cacttttctt cgcaggaagt tggtattttg
    65521 cgatacagga ttgatcttta gaataacccc atttcacatt aaactcgcct tccgcactca
    65581 agccagaaac ataagcaata ccatcttcac ctacaatgcc ggccatattt tcagtgtctt
    65641 tgctataact aatcacagcg ccgaagggga cgggtttacc ctctttctgt tttaaggtta
    65701 ctaatccttt atccccgctc ttcactgcca atgtcgcagg cacaacggct ccagctgtcg
    65761 gtacaaccaa ttgactagtt tgtggtaagt cgtaattctt acctatcgtt ttttcatcca
    65821 gaataacatc gtctcttcgg tatggctgca ctggcagtat tgcataccca cgaaaatcag
    65881 tactcagtcc cggccagcca ttaacgttaa cccccgttgc tccgggtgcc tctataaggg
    65941 ctaatgtccc atccacattt tgaccaaatg taagtccatc ccttgtaata agtatcccac
    66001 cgcttaaatt tgcacctagc tgtttcatgt tttcgctgga gctgtaatgg ctactaaact
    66061 gaccatatga tccatttttg ccaacttgca caatgttcat cgtgctcttc ggttgtgact
    66121 gatgagcgtt agttatattt gtcgctacat cccaccatac accaccacta ttctgacctg
    66181 ataatgaaac ctgatagtta ttaccctgct ctcccgaacg actcatctgt gaagacgcat
    66241 aaatttgatt ttcgtcaccc agcgataatg gcactgaaat acccacgctt aatttctgat
    66301 ctactttcca tttatgcgat gtcagcaagt cgttttttgc ccagttaatg gagaatgtta
    66361 tgcctctcca taccgtacta taactcaggc tggtagaccg cgtgtttcct gtgccccggg
    66421 aagaattata cctcgtggta ttgaagttca aattcccgat attaccaagg ggctgggata
    66481 aggatatcgt agagccatcg tgactattct tatctctttt tctgttccgt tcatcctgtg
    66541 cacttactgc ttcagtcagt gtggcataac ctgatgcata acggttcgtg gttatagac
//
LOCUS       DADLTF010000005        65606 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.005, whole genome shotgun sequence.
ACCESSION   DADLTF010000005 DADLTF010000000
VERSION     DADLTF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.005"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..146
                     /locus_tag="JN749_000334"
     CDS             <1..146
                     /locus_tag="JN749_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7366866.1"
                     /translation="QHKLLQTARLWLARHNGSFDTVDCRFDVVAFTGNEVEWIKDAFN
                     DHS"
     gene            166..756
                     /gene="diaA"
                     /locus_tag="JN749_000335"
     CDS             166..756
                     /gene="diaA"
                     /locus_tag="JN749_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HAY7366867.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            766..1341
                     /gene="yraP"
                     /locus_tag="JN749_000336"
     CDS             766..1341
                     /gene="yraP"
                     /locus_tag="JN749_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HAY7366868.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(1455..2495)
                     /locus_tag="JN749_000337"
     CDS             complement(1455..2495)
                     /locus_tag="JN749_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7366869.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLPGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(2568..3203)
                     /locus_tag="JN749_000338"
     CDS             complement(2568..3203)
                     /locus_tag="JN749_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAY7366870.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            3331..3849
                     /gene="yhbO"
                     /locus_tag="JN749_000339"
     CDS             3331..3849
                     /gene="yhbO"
                     /locus_tag="JN749_000339"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HAY7366871.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(3829..4272)
                     /locus_tag="JN749_000340"
     CDS             complement(3829..4272)
                     /locus_tag="JN749_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HAY7366872.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            4323..4625
                     /gene="yhbQ"
                     /locus_tag="JN749_000341"
     CDS             4323..4625
                     /gene="yhbQ"
                     /locus_tag="JN749_000341"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HAY7366873.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(4612..5115)
                     /locus_tag="JN749_000342"
     CDS             complement(4612..5115)
                     /locus_tag="JN749_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7366874.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(5109..5633)
                     /locus_tag="JN749_000343"
     CDS             complement(5109..5633)
                     /locus_tag="JN749_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HAY7366875.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVCDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            5842..6837
                     /locus_tag="JN749_000344"
     CDS             5842..6837
                     /locus_tag="JN749_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7366876.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            6846..7724
                     /locus_tag="JN749_000345"
     CDS             6846..7724
                     /locus_tag="JN749_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAY7366877.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            7805..8812
                     /locus_tag="JN749_000346"
     CDS             7805..8812
                     /locus_tag="JN749_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAY7366878.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(8930..10174)
                     /gene="mtr"
                     /locus_tag="JN749_000347"
     CDS             complement(8930..10174)
                     /gene="mtr"
                     /locus_tag="JN749_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7366879.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(10328..12217)
                     /locus_tag="JN749_000348"
     CDS             complement(10328..12217)
                     /locus_tag="JN749_000348"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HAY7366880.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(12210..12290)
                     /gene="yrbN"
                     /locus_tag="JN749_000349"
     CDS             complement(12210..12290)
                     /gene="yrbN"
                     /locus_tag="JN749_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HAY7366881.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(12397..13281)
                     /gene="nlpI"
                     /locus_tag="JN749_000350"
     CDS             complement(12397..13281)
                     /gene="nlpI"
                     /locus_tag="JN749_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HAY7366882.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEHTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(13390..15525)
                     /gene="pnp"
                     /locus_tag="JN749_000351"
     CDS             complement(13390..15525)
                     /gene="pnp"
                     /locus_tag="JN749_000351"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAY7366883.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(15772..16041)
                     /gene="rpsO"
                     /locus_tag="JN749_000352"
     CDS             complement(15772..16041)
                     /gene="rpsO"
                     /locus_tag="JN749_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAY7366884.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(16190..17134)
                     /gene="truB"
                     /locus_tag="JN749_000353"
     CDS             complement(16190..17134)
                     /gene="truB"
                     /locus_tag="JN749_000353"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAY7366885.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(17134..17535)
                     /gene="rbfA"
                     /locus_tag="JN749_000354"
     CDS             complement(17134..17535)
                     /gene="rbfA"
                     /locus_tag="JN749_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAY7366886.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(17699..20347)
                     /gene="infB"
                     /locus_tag="JN749_000355"
     CDS             complement(17699..20347)
                     /gene="infB"
                     /locus_tag="JN749_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAY7366887.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(20372..21859)
                     /gene="nusA"
                     /locus_tag="JN749_000356"
     CDS             complement(20372..21859)
                     /gene="nusA"
                     /locus_tag="JN749_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAY7366888.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(21887..22339)
                     /gene="rimP"
                     /locus_tag="JN749_000357"
     CDS             complement(21887..22339)
                     /gene="rimP"
                     /locus_tag="JN749_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAY7366889.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(22546..22622)
                     /locus_tag="JN749_000358"
     tRNA            complement(22546..22622)
                     /locus_tag="JN749_000358"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22586..22588),aa:Met,seq:cat)
     gene            22970..24313
                     /gene="argG"
                     /locus_tag="JN749_000359"
     CDS             22970..24313
                     /gene="argG"
                     /locus_tag="JN749_000359"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HAY7366890.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(24321..25946)
                     /locus_tag="JN749_000360"
                     /pseudo
     CDS             complement(24321..25946)
                     /locus_tag="JN749_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(26405..26491)
                     /locus_tag="JN749_000361"
     tRNA            complement(26405..26491)
                     /locus_tag="JN749_000361"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26455..26457),aa:Leu,seq:gag)
     gene            complement(26506..26838)
                     /gene="secG"
                     /locus_tag="JN749_000362"
     CDS             complement(26506..26838)
                     /gene="secG"
                     /locus_tag="JN749_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAY7366891.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(27066..28403)
                     /gene="glmM"
                     /locus_tag="JN749_000363"
     CDS             complement(27066..28403)
                     /gene="glmM"
                     /locus_tag="JN749_000363"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAY7366892.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRVLQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(28396..29244)
                     /gene="folP"
                     /locus_tag="JN749_000364"
     CDS             complement(28396..29244)
                     /gene="folP"
                     /locus_tag="JN749_000364"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HAY7366893.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(29334..31268)
                     /gene="ftsH"
                     /locus_tag="JN749_000365"
     CDS             complement(29334..31268)
                     /gene="ftsH"
                     /locus_tag="JN749_000365"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAY7366894.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(31368..31997)
                     /gene="rlmE"
                     /locus_tag="JN749_000366"
     CDS             complement(31368..31997)
                     /gene="rlmE"
                     /locus_tag="JN749_000366"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HAY7366895.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            32123..32416
                     /gene="yhbY"
                     /locus_tag="JN749_000367"
     CDS             32123..32416
                     /gene="yhbY"
                     /locus_tag="JN749_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAY7366896.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKKRKISLPR"
     gene            complement(32572..33048)
                     /gene="greA"
                     /locus_tag="JN749_000368"
     CDS             complement(32572..33048)
                     /gene="greA"
                     /locus_tag="JN749_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAY7366897.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            33296..34729
                     /gene="dacB"
                     /locus_tag="JN749_000369"
     CDS             33296..34729
                     /gene="dacB"
                     /locus_tag="JN749_000369"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HAY7366898.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(34915..36087)
                     /gene="cgtA"
                     /locus_tag="JN749_000370"
     CDS             complement(34915..36087)
                     /gene="cgtA"
                     /locus_tag="JN749_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HAY7366899.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(36103..37068)
                     /locus_tag="JN749_000371"
     CDS             complement(36103..37068)
                     /locus_tag="JN749_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7366900.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(37195..37452)
                     /gene="rpmA"
                     /locus_tag="JN749_000372"
     CDS             complement(37195..37452)
                     /gene="rpmA"
                     /locus_tag="JN749_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAY7366901.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(37473..37784)
                     /gene="rplU"
                     /locus_tag="JN749_000373"
     CDS             complement(37473..37784)
                     /gene="rplU"
                     /locus_tag="JN749_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAY7366902.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            38043..39014
                     /gene="ispB"
                     /locus_tag="JN749_000374"
     CDS             38043..39014
                     /gene="ispB"
                     /locus_tag="JN749_000374"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HAY7366903.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            39242..39520
                     /gene="sfsB"
                     /locus_tag="JN749_000375"
     CDS             39242..39520
                     /gene="sfsB"
                     /locus_tag="JN749_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HAY7366904.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(39568..40827)
                     /gene="murA"
                     /locus_tag="JN749_000376"
     CDS             complement(39568..40827)
                     /gene="murA"
                     /locus_tag="JN749_000376"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAY7366905.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(40882..41136)
                     /gene="ibaG"
                     /locus_tag="JN749_000377"
     CDS             complement(40882..41136)
                     /gene="ibaG"
                     /locus_tag="JN749_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HAY7366906.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(41296..41589)
                     /gene="mlaB"
                     /locus_tag="JN749_000378"
     CDS             complement(41296..41589)
                     /gene="mlaB"
                     /locus_tag="JN749_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HAY7366907.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(41589..42224)
                     /gene="mlaC"
                     /locus_tag="JN749_000379"
     CDS             complement(41589..42224)
                     /gene="mlaC"
                     /locus_tag="JN749_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HAY7366908.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(42243..42794)
                     /gene="mlaD"
                     /locus_tag="JN749_000380"
     CDS             complement(42243..42794)
                     /gene="mlaD"
                     /locus_tag="JN749_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HAY7366909.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(42799..43581)
                     /gene="mlaE"
                     /locus_tag="JN749_000381"
     CDS             complement(42799..43581)
                     /gene="mlaE"
                     /locus_tag="JN749_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HAY7366910.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(43589..44398)
                     /gene="mlaF"
                     /locus_tag="JN749_000382"
     CDS             complement(43589..44398)
                     /gene="mlaF"
                     /locus_tag="JN749_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HAY7366911.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            44608..45573
                     /locus_tag="JN749_000383"
     CDS             44608..45573
                     /locus_tag="JN749_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HAY7366912.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            45587..46573
                     /gene="kdsD"
                     /locus_tag="JN749_000384"
     CDS             45587..46573
                     /gene="kdsD"
                     /locus_tag="JN749_000384"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HAY7366913.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            46594..47160
                     /gene="kdsC"
                     /locus_tag="JN749_000385"
     CDS             46594..47160
                     /gene="kdsC"
                     /locus_tag="JN749_000385"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HAY7366914.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            47157..47732
                     /gene="lptC"
                     /locus_tag="JN749_000386"
     CDS             47157..47732
                     /gene="lptC"
                     /locus_tag="JN749_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HAY7366915.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            47701..48258
                     /gene="lptA"
                     /locus_tag="JN749_000387"
     CDS             47701..48258
                     /gene="lptA"
                     /locus_tag="JN749_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HAY7366916.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            48265..48990
                     /gene="lptB"
                     /locus_tag="JN749_000388"
     CDS             48265..48990
                     /gene="lptB"
                     /locus_tag="JN749_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HAY7366917.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            49038..50471
                     /gene="rpoN"
                     /locus_tag="JN749_000389"
     CDS             49038..50471
                     /gene="rpoN"
                     /locus_tag="JN749_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAY7366918.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            50494..50781
                     /gene="hpf"
                     /locus_tag="JN749_000390"
     CDS             50494..50781
                     /gene="hpf"
                     /locus_tag="JN749_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HAY7366919.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            50899..51390
                     /gene="ptsN"
                     /locus_tag="JN749_000391"
     CDS             50899..51390
                     /gene="ptsN"
                     /locus_tag="JN749_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HAY7366920.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            51436..52290
                     /gene="rapZ"
                     /locus_tag="JN749_000392"
     CDS             51436..52290
                     /gene="rapZ"
                     /locus_tag="JN749_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAY7366921.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            52287..52559
                     /gene="npr"
                     /locus_tag="JN749_000393"
     CDS             52287..52559
                     /gene="npr"
                     /locus_tag="JN749_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HAY7366922.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            52772..53404
                     /gene="yrbL"
                     /locus_tag="JN749_000394"
     CDS             52772..53404
                     /gene="yrbL"
                     /locus_tag="JN749_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HAY7366923.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(53401..54129)
                     /gene="mtgA"
                     /locus_tag="JN749_000395"
     CDS             complement(53401..54129)
                     /gene="mtgA"
                     /locus_tag="JN749_000395"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HAY7366924.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(54126..54779)
                     /gene="elbB"
                     /locus_tag="JN749_000396"
     CDS             complement(54126..54779)
                     /gene="elbB"
                     /locus_tag="JN749_000396"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HAY7366925.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(55009..57345)
                     /gene="arcB"
                     /locus_tag="JN749_000397"
     CDS             complement(55009..57345)
                     /gene="arcB"
                     /locus_tag="JN749_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HAY7366926.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEKSTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(57441..58370)
                     /locus_tag="JN749_000398"
     CDS             complement(57441..58370)
                     /locus_tag="JN749_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAY7366927.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            59045..63505
                     /gene="gltB"
                     /locus_tag="JN749_000399"
     CDS             59045..63505
                     /gene="gltB"
                     /locus_tag="JN749_000399"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HAY7366928.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPTTTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            63518..64936
                     /locus_tag="JN749_000400"
     CDS             63518..64936
                     /locus_tag="JN749_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7366929.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            65120..65599
                     /locus_tag="JN749_000401"
     CDS             65120..65599
                     /locus_tag="JN749_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HAY7366930.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        16043 a        17826 c        16232 g        15505 t
ORIGIN      
        1 aacaacacaa attattacag actgcccgct tgtggctcgc gcgtcataat gggagttttg
       61 atactgtgga ttgccggttc gatgtggtag ccttcaccgg gaatgaggtt gagtggatta
      121 aggatgcctt taatgaccac tcataattaa ggtttaagga ttagcgtgca agaaagaatt
      181 aaagcttgct tcactgaaag cattcaaact caaattgcgg cggcagaggc gcttccggat
      241 gccatctccc gtgcagccat gacgctggtt cagtctctgc tcaatggcaa caaaatcctc
      301 tgttgtggta atggaacttc cgctgccaat gcacagcatt ttgctgccag catgatcaac
      361 cgtttcgaaa cggagcggcc cagcttacct gccattgcac taaatactga taatgttgtc
      421 ttaacggcga ttgccaacga tcgcttacat gatgaagtgt atgcaaaaca ggtgcgggcg
      481 ctgggtcatg cgggagatgt attgttagcc atttccaccc gtggcaacag ccgcgatatt
      541 gttaaagcag ttgaagccgc cgttacgcgt gatatgacca ttgtggcatt gaccggctat
      601 gacggcggcg aacttgcagg tttgttaggg ccacaggatg tggagatccg cattccttcg
      661 catcgtagtg ctcgcattca ggaaatgcat atgctgacgg taaattgcct gtgcgatctg
      721 atcgataaca cgcttttccc tcaccaggat gattaaggag aatacatgaa ggcattatcg
      781 ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt gtgttgccgc tgccgtagtg
      841 ggtaccgctg ctgtgggtac caaagccgca actgacccac gcagtgtcgg cacccaggtg
      901 gacgatggta ccctggaagt gcgcgtgaac agcgcattgt cgaaagacga acagattaag
      961 aaagaaaccc gtattaatgt cacggcctat cagggcaaag tgctgctggt tgggcagtca
     1021 ccaaatgctg aactttcggc tcgcgccaaa cagattgcta tgggcgtaga cggtgccaac
     1081 gaagtgtata acgagattcg tcagggccag ccgattggtc tgggcgaagc atctaacgat
     1141 acgtggatca ccaccaaagt gcgttcgcag ctcttaacca gcgacctggt gaaatcgtcc
     1201 aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga tggggctggt aactgaacgt
     1261 gaagcgaaag cggcggcaga tattgccagc cgggtgagcg gcgtaaagcg ggtaaccaca
     1321 gcgtttacgt ttattaaata gcagcccctt gtaatgcctg atgcgacgct tgccgcgtct
     1381 tatcaggcct acaacgatgc aaaccgtagg tcggataagg cgtttacgcc gcatccgaca
     1441 tgttttccct caaatcagaa caacagcgcc agcccgccga caatcacacc agacactgcc
     1501 accatcgccc ctgtcagcca taaggctttc gccgggaacg ctttgcgcag cataatcagt
     1561 gacggcaaac tcactgccgg gagcgtcatc aacaatgcca gcgccggagc ggttcccata
     1621 cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat gacaaacaag
     1681 catcctgcta ccgccatcgc caccacccac atcaggctgt tatcgacagc accatcggca
     1741 tgggggaata accagacgcg tgcggcaccc aacaccagta ctgcaaggat gtaaaccggg
     1801 atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc ggctaaaaaa accgctctgt
     1861 gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg cacccatttt
     1921 tgcaccagcg tcgcaatcag caacaccatc accagcccgg ccaccagacg aatcgccgca
     1981 aaaccccagc cgaggacaaa ccccataaac accagcgtcg ccgggtttaa caccggattg
     2041 cccatccaga atgccagcgc accgcccatc gatacctgtt ggcgacgcat tcccgccgcg
     2101 accggagccg cacagcaggt acacatcatg cccggcagcg aaaacagcgt tcccggcagc
     2161 gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg gatcagcacc
     2221 tgaatcaacg aaccgaggat cacccccagc accgccgctt tccatactgc gaggaaatag
     2281 atcatcgcgt aatccaacgc cgcttgccac ggattagcat ccgcctgcgc aaggatagat
     2341 ttaccgatac tgtgggtttc ggctgcagta aacgctttgc cgtagtaagg ttgccatttc
     2401 acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca ccactgaatg
     2461 ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa taagttatga
     2521 taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta ttcagctctt
     2581 ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat gctcaggccg catcgcctct
     2641 gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc tggtaacaaa
     2701 cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc gcccagtaac
     2761 atcgacgggc gagcaatggt caatttcggc cagttctggg caattaatgc ttcttccatc
     2821 tcccctttga cgcggttata gaaaaacggc gagtgggcat tggcacccat agcactgacc
     2881 accagcatat gctgcgcgcc cagtcgccgc ccggttaatg cggtatccac taccagcgtg
     2941 taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt gccgagacaa
     3001 caaaatacaa tgtcgatggg ttcggtgacc tgcgccaacg cgtcactcag ttgcggatca
     3061 tggggattaa acaccccggg catatcgccc aacggacgtc gcgtcggcgc agcaatggcg
     3121 ttaactttcg gttcattaat caacatccgc agcaggtgac cgcccaccag ccccgttgcg
     3181 cctgtaatca gtacctgact catcttcgct cctttacaga attgtctgcc ttgcgctccc
     3241 cggctcatgc accagtctta atagaccatg aggtaattat ccccgattgt ggaaaattcg
     3301 cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt tttaatcact
     3361 gatgaatttg aagattcaga atttacttca cccgcagacg agttccgtaa agccggacac
     3421 gaagtgatta ctattgaaaa acaagcgggt aaaacggtga aaggcaaaaa aggagaagcc
     3481 agcgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga tgccctgttg
     3541 ctaccgggtg gtcattcacc ggattatctg cgtggcgaca accgttttgt cacctttacc
     3601 cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc gcagctgctg
     3661 atcagcgccg atgtgattcg ggggcgcaaa ctgaccgcag ttaaaccgat cattattgat
     3721 gttaaaaatg cgggcgtgga attttacgat caggaagtcg tggttgataa agatcagctg
     3781 gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt acgcctgctc
     3841 ggtgcctgag tcgcgtagcc aaatcatttt tttaccaaag cccagcgtgt tgtcggtgaa
     3901 tttgatttca tcgagccgga tttcccacac cggggccgac agcattctgg caaccggaaa
     3961 gcgacgattg tacgccttgc gcgcgaggtc gctttcctca ccttccagcc tgcggatctc
     4021 acctttaaac tgcacaccgc gaattaacgc taccgttttc ggctgaccgt ttaccgttcc
     4081 ggcaaccgca gcctgcggcc cactcatctg cgcgtggcgc gttttttctt ccgtcaaaat
     4141 gtagaaggcg actttctgcg catcaaaaag ataaaaggca ttagcgcacc agagttcccc
     4201 ttcctgctgc acacaccagg tgacaacatg ttgttttgcc agccaacggc tgatggcgat
     4261 gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat gcgatgcaga
     4321 cgatgacccc ctggtttctt tacttgatcc gtaccgccga caataagctt tataccggga
     4381 tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg gcgaaagcac
     4441 tgcgcgggaa aggagaactg acgctggcgt tttctgcgcc agtgggcgac cgttcgctgg
     4501 cactacgggc ggaatatcgc gttaagcaac tgaccaaacg gcaaaaagag cgtctggtag
     4561 cggaaggcgc agggtttgca gaactgttga gcagcctgca aaccccggag attaaaagcg
     4621 attgaaatgc tcgtgatact caaccagacc ggttacgcca ttcagcgcgt catccgctaa
     4681 acgatgtacc tggaaggcgc tttcagtgcc cggccagcgg caacgcagat cgtgatgcgc
     4741 cgccagttca aagccgaaac gactgtacag cgccggatcg cccagcgtca ccactgcggc
     4801 atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg ccagcccctg
     4861 tccacggtat ttttcatcaa ccgccaacgg tgccatgccg acccattgca ggtcttcgcc
     4921 ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt catctgtcgc
     4981 caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct tcgcttccgc
     5041 atcactttcg aatgagcgac gcagcagggc atcaataccc ggcgcatcaa tgggaatttc
     5101 tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc ggttttcatt
     5161 cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt cggcatttgc
     5221 tccagttcaa tggcgtccat caggtttttc acatacaccc ccagctccgt atcgccttca
     5281 atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc cgcaatcatc
     5341 agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgcgttctg gctgaccacc
     5401 agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacacac atgaatactt
     5461 aaccagcggc cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg gaattgccag
     5521 cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt taccggtaca
     5581 ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa cacgagtttt
     5641 actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat agcggtataa
     5701 atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg ccttaaatca
     5761 aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg cgttttgata
     5821 gcgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct cccggcgctt
     5881 aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga tgataccaat
     5941 gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc ggtgagtttt
     6001 gtccatcaac atcgccgcaa acttcacatc tcgattaaca cttttgcgca tccggacggt
     6061 tacgcccgtt ggcagcgtgc cgtggatatg gcggcgcagc tgggtgccga cgcgctgatc
     6121 ctcgccgacc tcgccatgct ggagtacgcc gccgagcgtt acccgcatat tgaacgtcat
     6181 gtgtcagtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca tcgccatttt
     6241 gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa acagctggca
     6301 cgggttactc ctgtcccgct ggaagtgttt gctttcggca gcctgtgcat tatgtcggaa
     6361 ggccgttgct atctgtcgtc gtatctgacg ggtgagtcgc ccaacactgt gggtgcgtgt
     6421 tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc ccgcctgaac
     6481 gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac gctgtgtaaa
     6541 gggcgttatc tggtggacgg cgagcgctat cacgcgctgg aagaaccaac cagtctcaat
     6601 accctggaac tgctgccgga gttaatggct gcgaatattg cttcggtgaa aattgaaggc
     6661 cgccagcgta gcccggcgta tgtcagccag gtggcgaaag tctggcgtca ggctatcgac
     6721 cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga gacgctcggg
     6781 tcgatgtccg aaggcacgca aaccaccctt ggcgcgtatc accgtaaatg gcagtgagaa
     6841 gagcaatgaa atattcctta gggccagtgc tgtggtactg gccaaaagag acgctggaag
     6901 atttttatca gcaagccgcc gccagcagcg ccgacgtgat ttatcttggt gaagcggtat
     6961 gcagcaagcg ccgggcaacc aaagttggcg actgtctgga gatggcaaaa tcgctcgccg
     7021 gaagtggcaa gcagattgtg ctctccacgc tggcgctggt gcaggcatcc tctgaactgg
     7081 gcgaactgaa acgctatgtt gagaacggtg agtttctgat tgaagccagc gatctcggcg
     7141 tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg ctgaactgct
     7201 acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg tgtatgccgg
     7261 tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag ctgggcattc
     7321 gcaaccagtt tgaagtggaa gtcctgagct acggtcatct accgctggcc tactccgccc
     7381 gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc tgctgcatta
     7441 agtatccgaa cgggcgcaac gtgctgtcgc aggaaaacca acaagtgttt gtactcaatg
     7501 gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctggca tccatgcagg
     7561 gattggttga tgtagtccgc ctgtcaccgc agggcactga cactttcgcg atgctcgacg
     7621 ccttccgcgc taatgaaaat ggcgctgtgc cactgccgtt gacggcaaac agcgactgta
     7681 acggctactg gcggcggctg gcaggactgg agctgcaagc ctaagtaaat agctcacttt
     7741 gttaacaact ttaactattc tttaatgcag tattaaagat taatcggtaa caaagtgagc
     7801 tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca tacccgaagg
     7861 ctcttcagcg cgggaagcgt tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg
     7921 cggctatcat cgctactggc tggcagaaca ccacaatatg accggcattg ccagtgctgc
     7981 aacatcggta ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg
     8041 cggcgtgatg ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg gcacactcaa
     8101 tacgctctat ccggggcgaa tcgatttggg gctgggtcgt gctccgggta gtgaccaacg
     8161 gacaatgatg gcgctacgtc gtcatatgag cggcgatatt gataatttcc cccgcgatgt
     8221 cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc
     8281 aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gtgcacaact
     8341 ggcggcgcag cttggtctgc catttgcgtt tgcctcacac ttcgcgccgg atatgttgtt
     8401 ccaggcgctg catctttatc gcagtaactt caaaccgtcg gctcggctgg aaaaaccata
     8461 cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctgtt
     8521 tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccgggc aactgccgcc
     8581 gccgattcaa aatatggatc agttctggtc accgtctgag cagtatgacg tgcagcaggc
     8641 gctgagtatg tcgttggtag gcgataaagc gaaagtgcgt catggtttgc agtcgatcct
     8701 gcgcgaaacc gacgctgatg agattatggt taacgggcag attttcgacc accaggcgcg
     8761 cctgcattcg tttgagctgg cgatggatgt taaggaagag ttgttgggat agtgtgtctt
     8821 aacgcgggaa gccttatccg agctggcaac gctgtcctac gtaggcctga taagcgaagc
     8881 gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca
     8941 ccggcagtaa attaaagctc gataaaatat gcaccagtgc gttgccgacg ccaaacacca
     9001 gaatcagcgc aatcatcggc ttgccacccc agacgcggaa tttcgggctg ccaaagcgtt
     9061 tacgcgatgc gcgggccagc agtgccggaa caattgccgc ccagatggta gccgctaaac
     9121 cagcataacc aatggcatac aggaatccgt tcgggaacag cagaccaccc acaacaggcg
     9181 gggcaaaggt cagcaatgcc gttttcaagc ggcccacagc cgagtcgtcg aaaccaaaca
     9241 gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa
     9301 agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg
     9361 cttgtaccag cacatcaata ttaccgccct tctcggcgat gccgataaac tccggacgag
     9421 ggatgttgcc cattgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac
     9481 cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggcttg
     9541 gcacgttacc gtgataacca aacgacgcca agcagaacgg cagggtcatc agcaaatacg
     9601 gcgcataaga tgcattgctt tcggcaacgt tgaacaatgt cgcaggctgc acatgcccca
     9661 gcaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagcaca atcgccgtca
     9721 tgcggctgac ggctttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa
     9781 aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaatcg
     9841 aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatgc
     9901 cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac
     9961 cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc
    10021 aggtaaagat cagcgctgcc attgaccaga aaaaccacgc cccggacatg accactggca
    10081 gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg
    10141 acggtgacgt ttgggtggtg gttagtgttg ccatgagggc ttctctccag tgaaaaatag
    10201 tgcgactgcg ttgttatgca ttgcactgta ccagtacatg agtacaaaag acagaaaaaa
    10261 agccccgatg gttaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca
    10321 gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggtg
    10381 cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacgacca ccttcacggc
    10441 gttcaccgct gaagttacga ccgccttcac gacgctcgcc accgaaacca cgaccaccgc
    10501 cacgaagctc accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct
    10561 tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca
    10621 gttcgatggt ggagtgagaa gcaaacagct tgatgttacc gatgtaacgg ctgctgatgt
    10681 cgccttcgtt agcaatcgcg ccaacgatat gacgaacttc aacaccatca tcgcggccca
    10741 cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt
    10801 cacggtcgcc acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac
    10861 ggaattcacg tttcggacgc atcggcgcat ctggcggaac aatcagagta cgttcaccct
    10921 gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag
    10981 tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct
    11041 gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca
    11101 gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct
    11161 cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga
    11221 tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaaccacc aggctgatac
    11281 gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat caggatgtcc agacgaccgt
    11341 ctttaaggcg ttccagtgtc tgctcacgca gcgcctggtt catgtcaccg ttcagcgcgg
    11401 cgctattgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac
    11461 gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt
    11521 ttttacgcat accccacaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc
    11581 tggactgaat acgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg
    11641 cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa
    11701 tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca
    11761 gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg
    11821 gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct
    11881 ggccgccgta cagggcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg
    11941 cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt
    12001 tcagctcagg atcaagattc tgcagcagag gtaaagagaa tgctgcagtt tttccgctcc
    12061 ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct
    12121 gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca
    12181 ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta
    12241 atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt
    12301 gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg
    12361 caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag
    12421 gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa
    12481 gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc
    12541 cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga
    12601 ggtgttatcg gttgcgtccg ccttgagcct ttccattaac gtgtgttcgc taatgttgcc
    12661 caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg
    12721 tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag
    12781 actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa
    12841 cttgtcacga ccgccgtaat ataatgcaat cccgcgattc aagtgcgcgt agttgtaagt
    12901 tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg
    12961 cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc
    13021 ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg
    13081 ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc
    13141 catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc
    13201 acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca
    13261 ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt
    13321 tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa
    13381 atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga
    13441 ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttccagaac
    13501 tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc
    13561 agcgatttga gagatgtgta ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc
    13621 aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt
    13681 gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcaatctt
    13741 cacagtaccg tcatcttcga tttcgatggt ggtgccagtt tcttcggtca gggcacggat
    13801 aacagagccg cctttaccga taacgtcttt gatcttgtcc gggttgatct tgatggtatg
    13861 gatacgcggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac
    13921 gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt
    13981 ggtgatacct tcaattttga tatccatctg caatgcagag ataccctcgc gggaacccgc
    14041 aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac
    14101 gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgatcgg
    14161 cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaggaacc
    14221 gttggattca gtgatttcag acacaacacg tacggtgtac gggaatttgt ccatatccgg
    14281 catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg
    14341 agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa
    14401 ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cagtacccag
    14461 cgttgcggta accagcgcct gcgtttcacc acgggtgaac agcgcagaac cgtgagtacg
    14521 cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat
    14581 acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatcg cgtgcagaat
    14641 ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc
    14701 agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc
    14761 gctcaggcga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc
    14821 cggctgccag tcccaacgtg gtttaccggc ttctttcacc agttcattga tgttctgaat
    14881 aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctggt cttcgctcag
    14941 cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc
    15001 cagtttgctc tctttcaact cgtcctgagt cgggttcagt acgtactggt cattgatgta
    15061 acctacgcga gcagcaccaa tcggaccatt gaacggaata ccagacagag acagtgctgc
    15121 ggaagcacca atcatcgcga cgatatccgg gttaacttgc gggttaacag aaaccacggt
    15181 ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc
    15241 aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg ccttcacgac ggaagaagct
    15301 acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa
    15361 gtcctgacct ggtttggctt ttttctggcc aacaacggta acaaataccg cggtgtcatc
    15421 catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac
    15481 ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct
    15541 ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc
    15601 gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac
    15661 ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt
    15721 cagcttgaaa aaaggggcca ctcaggcccc tttttctgaa actcgcaaga attagcgacg
    15781 cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc
    15841 gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt
    15901 tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg
    15961 aacttcagta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt
    16021 tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat
    16081 ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta
    16141 agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt
    16201 attcaaccac caggcgacga ggcgcaacgc ggccttcatc gtcaatttca cccataccga
    16261 taaatttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg
    16321 tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg
    16381 ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg
    16441 gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcacaatcc
    16501 gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac
    16561 cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa
    16621 tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg
    16681 ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct
    16741 gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca
    16801 gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt
    16861 cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg
    16921 agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgccag
    16981 ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca
    17041 ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt
    17101 taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt
    17161 aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc
    17221 ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc
    17281 tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt
    17341 aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc
    17401 gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc
    17461 acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc
    17521 aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa
    17581 attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc
    17641 aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt
    17701 aagcgatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg ccagtgcgga
    17761 cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt
    17821 catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca
    17881 gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg
    17941 caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt
    18001 tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga
    18061 tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt gcagaggcat
    18121 cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg
    18181 tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca
    18241 gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga
    18301 tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt
    18361 tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac
    18421 gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca
    18481 ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt
    18541 tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca
    18601 gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt
    18661 cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct
    18721 gcagcaggat agcgtccagc agttcatcga taccagtacc cgctttcgca gacacatgta
    18781 cgaactggct ttcaccgccc cactcttccg gcaggatgcc gtactgggag agttcgttct
    18841 taacgcgatc cggatcggct tctggtttat cgatcttgtt cactgcaacg accaccggaa
    18901 cctgcgccgc tttcgcgtgc tggattgctt cgatcgtctg cggcattacg ccgtcgtcgg
    18961 cagcaacaac caggactacg atgtccgttg cctgcgcacc acgagcacgc attgaagtaa
    19021 acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt
    19081 atgcaccaat gtgctgagta atgccgcccg cttcgccaga ggccactttc gttgaacgaa
    19141 tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg
    19201 cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt
    19261 tttcacgacg caggataact ttatggccca tctcttcagc aaccagctgt gcggtttcct
    19321 gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct
    19381 gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatagtt tcgccgatca
    19441 caacgtcacg gttaacggcc tgagcaggct tctggaagcc ttgctgcagc gaagaacctt
    19501 tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg
    19561 attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac
    19621 ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag
    19681 tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt
    19741 ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgacgcg
    19801 cttcttcttc agctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag
    19861 cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt
    19921 cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcggcctca cgttcagctt
    19981 tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctg
    20041 caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg cggacttcga
    20101 tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg cgtgttttac
    20161 gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca atcaaagtct
    20221 gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca tcagcaaatt
    20281 gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta atcgttacat
    20341 ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac cagcaaatat
    20401 tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca
    20461 gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt
    20521 tgaatgccaa atcacgatct accccttcaa ggttcagcag atcgtcagcc ggtttgttat
    20581 caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc
    20641 gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca
    20701 cataggccag ttcttccagc gtcgagaaac cttcttctac cagaacagtc gcgaagtctt
    20761 cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct
    20821 tagcctgcag gtcgtcaacg gtcattacgt tgagttccca accgctcagc tgcgaagcca
    20881 gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccggct tcaacggcga
    20941 tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg
    21001 cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccgc
    21061 ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg
    21121 gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc gggtcgcgag
    21181 ccgctgcttt aatttcaatc acttcttcgc cgatttccgg cacttcaata cggaacagtt
    21241 cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc
    21301 gaacggaata aagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca
    21361 tatcttcgcg caggatcacg gcttcagcat tgttgcccag atccagagag atgttgtcgc
    21421 ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa
    21481 ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg
    21541 tggtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaagttca
    21601 ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt
    21661 catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga tcgatctgta
    21721 cacggacgtc gatctcttgc tcatattttt tctttgttgc tgtcgccagc gcgctttcca
    21781 atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag
    21841 ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa aagtggggaa
    21901 ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct tcgacggtaa
    21961 ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga cggttttgta
    22021 ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag tgttcagccg
    22081 tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca acggtgatgg
    22141 gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa tcatcaacat
    22201 tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg cgaataaatt
    22261 cgatgcctac cagttcaaaa cccagggcct caactggcgc agtaatcatc tctgttaatt
    22321 tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta aagcccagtt
    22381 attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa ctgaacccta
    22441 taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc cactacgaag
    22501 gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt gcgggggccg
    22561 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccagactg ctccaccccg
    22621 cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa atgcaaatac tgctgggatt
    22681 tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg ccacattttg
    22741 tcaacgttta ttgctaatca cgtgaatgaa tatccagttc actttcattt gttgaatact
    22801 tttgccttcc cctgctctcc cttaagcgca ttattttaca aaaaacacac taaactcttc
    22861 ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa aattcagtga
    22921 gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta tgacgacgat
    22981 tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg gtctggacac
    23041 cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat atactgcaaa
    23101 cctaggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca tggaatacgg
    23161 cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag gtattgccgc
    23221 tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca acacgacgcc
    23281 gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag atggcgtgaa
    23341 tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct atcgttatgg
    23401 tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg actttattga
    23461 tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt tcgactacaa
    23521 aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa cgcatgaagc
    23581 gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta tgggcgtgaa
    23641 attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct ttgagcaagg
    23701 tcatccggtt gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga tgctggaagc
    23761 taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaga accgtatcat
    23821 cgaagcgaaa agccgtggta tttacgaagc tccggggatg gcactgctgc acattgcgta
    23881 tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc acgcgcatgg
    23941 tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg cgctgatgct
    24001 gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta ccctggagct
    24061 gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga cctacaagcc
    24121 agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc gtattggtca
    24181 attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg gttatgccaa
    24241 aactggcctg ctttcctcct ctgccacttc aggcctgccg caggtggaga atatggaaaa
    24301 caaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc ttgcttatcc
    24361 tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc atccacaacc
    24421 cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg ttccggcttt
    24481 gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa gatattgttt
    24541 tccgttgtgc gatccacgcc atccccaaga acggggctat aacagataaa catcgggaca
    24601 tgatatgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt cttcgtaggg
    24661 tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc ttttaataat
    24721 tcaaaaaact gacctagcaa actatctgtg taatgaatgg agttgtcata gcaggcatcc
    24781 tgatcgtcct gcggttgaaa cacgcgctgg attgcggata ggcgctacaa gccggttcat
    24841 ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc tgtaatgctt
    24901 gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt tccatggcgc
    24961 gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc gagcttagcc
    25021 agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg taattatgaa
    25081 tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc gcagtgtaag
    25141 gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg
    25201 gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca gactccccca
    25261 caatcaacac gtaggtatca atacttgtat ccctgactga taattgaaaa tacggcaccg
    25321 tgtttgcaat tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa tagttgagat
    25381 tgaaaaacgg cgtataggta gcaaatcacg acgctagtat atatggactg aacgcttttt
    25441 tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc gaaatgacaa
    25501 tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat tttattatta
    25561 ctgccaaaaa aaaaagggat aaaaaagcaa aagcacatag ataagggata tacatcccca
    25621 gcattttcac aacttcatcc ggatcactct gcagcacact aatcgcaaat ccatcattga
    25681 aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc gcagtcagta
    25741 acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt ttaacaacca
    25801 ggatggtcag gacgaagaac agcaatcgct gaaccatatg cgctccggaa gaggcgacta
    25861 agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat ttaaaatctt
    25921 ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa tataaacaat
    25981 tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgaacca tatcagaccg
    26041 aaacactgtt agttttttct ttttaaatga tgcgaccatt gcgttctcag taattaaata
    26101 acattatcaa tgcgtctcta aacattacgc agaacgtagc catcagtagt tatactgaca
    26161 ggagcaggat ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa ctggagttga
    26221 cacaataaag tgccaattat gtcagcagta gggaaaagtt tataactaag gcgtaccggc
    26281 accatcgttt caaggtacca gctacgagta aagcaactgg acgagataca gatacctgac
    26341 aaccattcat cagaccagga ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg
    26401 aatttggtac cgaggacggg acttgaaccc gtaagcccta ttgggcacta ccacctcaag
    26461 gtagcgtgtc taccaattcc accacctcgg cacggatact actttttagt tcgggatatc
    26521 gctggtcggc ttagccggag cagctggctg agtttgttcg gttttcgccg gtgcactcag
    26581 attttcccat tcgctacctc tattggtttt gttgctattg atgttaccca gcaccagact
    26641 gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc
    26701 acttgaacca aacagcgtag cggaagcgcc tgctccgaag gaggctccca tatcagcgcc
    26761 tttaccttgc tgcagcatga tcagaccaac aaggccaatt gccacaataa ggaaaactac
    26821 taaaagagct tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc
    26881 aaccaataaa gcgggagttt ttaatctttc ccactgaagc gggtgtgaat actaaccaaa
    26941 gcgaatgacc ttcgcaaggg caattttatc gcattgtatc aactgcggaa aaaaacagca
    27001 aaaccgatgt gttcgccgag aaaaaaggcg acaatcgccg cctttttagc cagttatcta
    27061 acgctttaaa cggcttttac tgcatcggcg atgcggtgtg caaattcagt cacctgcgct
    27121 tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg tgccggattt acgcagcaac
    27181 acgcgtccac ggttgcccag cgcagcttca acctctgcgg tcacggcttt aactgactca
    27241 tgctcaagcg gatcgccgct acctgcggtg taacgtacgt taaccagaat ctgcgggaac
    27301 attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt tacgtgccat cgccgccagc
    27361 acctgcaagc cagcaacgat gccgtcaccg gtagtggttt tatccagcag gatcacatga
    27421 ccggaattct ctgcaccgat acgccagcct ttctcctgca ttttttccag tacgtagcgg
    27481 tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag ttcaagcccc
    27541 atgttgctca tcaatgtacc cacagcgcca ccacgcagct ggccctgacg aagaccttca
    27601 cgcgcgatga tatacatgat ctgatcgcca tcgactttat tgccttcatg gtcaaccata
    27661 atcacgcgat cgccgtcgcc gtcgaaggca ataccgagat ccgctttttc agccagcaca
    27721 cgagcctgga gcacgcgaac gtcggtagcc cccacttcgg cattgatgtt tacaccgttt
    27781 ggctcacaac cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg
    27841 tgataagtcg caccgtttgc acaatccacc acaatcttca gttcactgag gctaagttcg
    27901 ttcgggaacg tggctttgca aaactcgata tagcgacccg cggcatcaac gatacggctg
    27961 gctttaccca gttctgccga atcaacgcag ctgatctcct tttccatttc cgcttcgatg
    28021 gcctcttcta ccgcatccgg cagtttagtg ccgtcgatag agaagaattt aatcccatta
    28081 tcgtagaacg ggttatgcga tgcagatatc acaattccgg cctctgcgcg gaaggtacgc
    28141 gtcagataag ccacggccgg tgttggcatc gggccagtga agagtgcgga aaggcccgct
    28201 gccgccagac ccgcttccag tgctgactcc agcatatagc cagaaatacg cgtgtcttta
    28261 ccaataataa tcttacggga gccgtggcgc gccagcactt tacccgcggc ccaacccagc
    28321 ttaagcacaa aatcaggtgt gatcggcgca tcccctacac gaccacgaat cccatcggta
    28381 ccgaaatatt tacgattact catagcgttt gttttccttt gcagacagag tggcttccac
    28441 cacccgcatc gcttctacgg tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg
    28501 cattgcggca atgaccgcac aggccagact accgctcagg cgctcggacg gccccacgtt
    28561 cagcagctgc ccaatcatcg attttcgtga catacccacc aacagcggca ggttgaaatg
    28621 gtgaaattca gccaggcgcg ccagtaatga atagttatgg gagagatttt taccgaaacc
    28681 gaatccgggg tcgagcaaca atttctcttt tgcgataccc gcctgctcgc aacgtgctat
    28741 ttgctcaata aagtagcgat tcacttctgc aaagacatcg tcatacttcg gagcttcctg
    28801 catggttttt ggatttccct gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc
    28861 cgcctccaga gcgccaggtt cggaaaggga gcggatatca ttaataatgt gagcgccaac
    28921 tttcgctgac tcacggatga cttctggttt ggatgtatcg accgagatcc agacttcgaa
    28981 gcgttgagca attgcctcaa ccacaggaat aacacgttgc aactcttctt caacgctaac
    29041 ttccgccgcc cctgggcgcg tggactcgcc accaacgtca atgatcgtcg cgccagcatt
    29101 gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc caccatccga
    29161 aaaggaatca ggcgtgacgt tgaggatccc cattacgtga ggatggctaa ggtccagtga
    29221 agtaccctgg gcaaagagtt tcatgatgtt atccctggta tgaattgata agaaaaaacc
    29281 ccggagcacg ccccggggtt ttcggtacaa atacagtcat ctgatgcggg aacttacttg
    29341 tcgcctaact gctctgacat ggtgttaccc gggttcggcg tacgcggttc atcaaccgga
    29401 cgaggagcct ttggactacc attgtcgcca gaattgttag aagcgcctgg ttcttcccag
    29461 cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata
    29521 gtctcatatt tcatgagagc atctttcatc gcatgcagaa tatccatatt gtcggtcaga
    29581 agctgacgcg cacgattata gttacgctca atcagtgctt tcacttcctg gtcgatgata
    29641 cgtgcagttt catcggacat atgtttcgct ttcgctacgc tacggccgag gaatacttca
    29701 ccttcttctt ccgcgtacag cagtggaccc aatttctcag agaagcccca ctgagtcacc
    29761 atgttacgtg ccaggttggt cgcaacttta atatcgttgg acgcaccggt agaaacatgt
    29821 tccggcccgt agatgatctc ttctgccaga cgaccaccgt acagcgtaga aatctggctt
    29881 tccagtttct gacggctggc gctgattgcg tcgccctcag gcaagaagaa agtcacaccc
    29941 agcgcacgac cgcgtgggat aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg
    30001 cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg attctttctg cgcttccgtc
    30061 atcaccatgg agcgacgttc cgcacccatc atgattttgt ctttcgcttt ctcgaactca
    30121 accatcgaca caacgcgttt gttgccacga gcagcgaaca gtgccgcttc gttcaccagg
    30181 ttcgccaggt cagcaccgga gaaaccagga gtaccacggg caatgattgc cgcgtcgata
    30241 tcgggtgcca atggtacgcg acgcatgtga actttcagga tctgctcacg accgcgaaca
    30301 tctggcaagc cgaccacaac ctggcggtcg aaacggccag gacgcaacag ggccgggtcg
    30361 agaacgtccg gacggttagt cgcggcgata acgatgatac cttcgttacc ttcgaagcca
    30421 tccatctcaa ccagcatctg gttcagagtc tgttcacgtt catcgtgacc accgcccaga
    30481 ccagcgccac gctggcggcc tacggcgtcg atttcatcga taaagatgat gcacggtgcc
    30541 gctttcttcg cctgttcgaa catgtcacga acacgggatg cacccacacc gacgaacatt
    30601 tctacgaagt cagaaccgga gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct
    30661 ttcgccagca acgttttacc ggtacccgga ggaccgacca tcaggacgcc tttcgggatc
    30721 ttaccgccga gtttctggaa gcggctcggc tcgcgcagat actctaccag ttcagcaact
    30781 tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc
    30841 gtcagcatgc gcgctttgct cttaccaaac gacatggcac ctttgccacc gccgccctgc
    30901 atttgacgca tgaagaagat ccagacacca atcagcaaca gcatcgggaa ccaggagatg
    30961 aagatagaag ccagcaggct tggttcttca ggcggttcac cgacaacctt gacgttcttg
    31021 gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt ataacggtta
    31081 ctatctttct tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg aacctggtcg
    31141 ttattgacct cttgtaggaa ggtagagtaa tccaccttac ggccattaga ctcgctgggc
    31201 ccaaagctct ggaatactga catcagcaca acggcaatga ccagccagag tattaggttt
    31261 ttcgccatgt cactcaaggg attaacctct tattacaact gtgttaaaaa cagcgtcagg
    31321 atactctata tccagcatct ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc
    31381 ccggtcgcta caatatacac ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact
    31441 ttgaccttcg taaacaggga gcgaatttcc cttagatact catcgaaacc ttcgccctgg
    31501 aacaccttca ctacaaaact gccacctggc gctaatacat cacgacacat ttctagcgcc
    31561 agttccacca gatacatggc acgggggata tccaccgccg gtgttccgct catgtttggt
    31621 gccatatcgg acatgacaac ctggactttg ctgtcgccaa cgcgctccag cagtgctttc
    31681 atcaccagtt catcacgaaa atcgccctga agaaagtcca caccaacgat aggatccata
    31741 ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa tttgggtgac cacatattgt
    31801 gaccaaccac ccggagcagc accaaggtcg acaaccgtca ttcccggttt aaagagtttg
    31861 tcactttgct gtatttcatc aagtttaaac caggcacggg aacgtaatcc ctttttctgt
    31921 gcctgttgaa catatttatc gctaaagtgt tcctgaagcc agcggctgga gctggcagaa
    31981 cgcttcttac ctgtcattta actttcccat ggggataact catcgtaacc aattgcgtaa
    32041 atttttacgc gcctatttgg tgatatatgg gagatggcgg tagaatgacc cgttttcaat
    32101 cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa caaaaacagc acctgaaagg
    32161 tctggcacat ccgctcaagc cagttgttct gcttggcagt aatggtttga ccgaaggggt
    32221 gctggccgag attgaacaag cgttagagca ccatgaactc atcaaggtga aaatcgccac
    32281 cgaagatcgc gaaacaaaaa ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg
    32341 taatgtacag gtcatcggta aaacgctggt gctttatcgc ccaactaaaa aacgtaaaat
    32401 ctcgctgcca cgctaagatt atcctaaagt tacacacatt tgctgtgtaa aacgaggggt
    32461 tttccgcagg caggagagca aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg
    32521 gcctttttcc tttctttaca atacatcaac atcttgagta ttgggtaatt cttacaggta
    32581 ttccacctta attacttcaa attctacttc gccgcccggc gttttgatga ccacaacatc
    32641 atcttcttct ttgccgatca ggccacgagc aataggcgag tttacagaaa tcaggttttg
    32701 tttaaagtca gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt cagaatccag
    32761 attcagcacc gttacggtag caccaaaaat aacgcgccca ttgttgggca ttttggtgac
    32821 atcaatcacc tgcgcgtttg acagcttggc ttcgatgtct ttgatacggc cttcgcagaa
    32881 gccctgctgt tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc catgctcacg
    32941 cgcttccgcg atagcagcaa tgatttcagg acggcgcaca gatttcagaa aatccagctc
    33001 ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata gcttgcattt gttatacctc
    33061 ttgaatattc ctgatggggc aagtcttcac ccaccccggc tgttcggcct gcccggcata
    33121 actcctcacc gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc caggtcagct
    33181 acaattcaca ttttgatggt cattttaccc tgaagttccc gaagggtcat cgtttacttt
    33241 atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg agattatgcg
    33301 attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa
    33361 tgttgatgag tacgttactc aactccccgc aggtgccaac cttgccctga tggtgcaaaa
    33421 agtcggtgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc tgcccgccag
    33481 tacccagaaa gtgattactg cgctggcggc gttgattcaa ctcggccccg attttcgttt
    33541 caccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg gtgacttagt
    33601 ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat attcgcaata tggtcgcgac
    33661 tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata cctccatttt
    33721 cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat gctttagcgc
    33781 tccgcctgct gccgccatag ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc
    33841 aaagcctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta cgatgttcag
    33901 ccaggtacgc accctccccc gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt
    33961 gccaggcgac ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt ctgagccgct
    34021 cccgttggct tttggcgtgc aggatggagc cagctatgcc ggtgcaattc tgaaagatga
    34081 gttaaaacag gcgggtatca cctggagcgg aacactgctg cgccagactc aggttaacga
    34141 acctggaacg gtagttgcca gtaaacagtc ggccccgctg cacgatctgc ttaagattat
    34201 gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatcg gccatgcgcg
    34261 cttcaatgtg cctggaacat ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca
    34321 gcaagccggt gtcgatattg gtaacaccat tattgccgat ggttcagggc tttcgcggca
    34381 taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc aacacgacaa
    34441 tgaacttaac tttatctcca tgctgccgct tgcgggctat gacggctctt tgcagtaccg
    34501 tgcaggtctg catcaggcgg gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca
    34561 gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa tggcatttgt
    34621 acaatatctt tctggctatg cagtaaaacc cgcggatcag cgtaatcgcc gtattccgtt
    34681 agtgcgtttt gaaagccgtt tgtacaaaga tatttatcag aacaattagt caaaaagaaa
    34741 ccccggcgca tggccggggt ttcagattat tgacaaagtg cgctttgttt atgccggatg
    34801 cggcgtgaac gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct
    34861 tgtaggcctg ataagtgtag cgcatcaggc ttatttggca ttggtcatta gtgattaacg
    34921 cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc
    34981 atcatcctct tcagcaatct cttcaagctg ctgacggtga taatcatccc acatgaattc
    35041 gactttctct ggctgtttcg cttcttcagc ctgcacgacc gggttttcaa tgataaaggt
    35101 catcacatcc cagcagagat ctttcacgcc cagtccgctc gccgcagaga tcagataata
    35161 tttatcttcc cagcccagcg cttcagcgat cgctttcgct ttctcttcgg cttctgcctt
    35221 atccagcaga tcgatcttgt tgaagactaa ccaacgcggt ttcgccgcca gatcctggct
    35281 gtatttttcc agctcgctga taataatacg cgcgttttca accggatcgg tgccgtcaat
    35341 cggatcgata tcgatgaggt gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg
    35401 aatgcccaga cctgcgcctt ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac
    35461 gaagctcttt tcgttgtcca ttcgtaccac acccagactt ggcaccagag tggtaaacgg
    35521 ataatccgcc actttcggtt tagccgccga taccgcacga ataaaggtcg atttacccgc
    35581 gtttggcatc cccaacatac cgacgtcagc cagcagcatc agctccagca gcagctcacg
    35641 cttatcgccc ggcgtaccgt tagttttctg ccgtggtgta cggttaactg acgatttgaa
    35701 acgggtattg cccagaccgt gccagccgcc cttagcaacc agcagacgct gaccgtgttt
    35761 agtcatatca cccatggttt caccagtacc ctggtcgatt acacgcgtac ctaccggcac
    35821 tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc
    35881 gcgctctgca cggaaagatt tttcaaaacg ataatcgata agcgtgttca ggttctcgtc
    35941 ggcttccatc catacgtcac caccatcacc gccgtcgccg ccatccgggc cgcctttcgg
    36001 aatatacttt tcgcggcgga agctcacgca accattaccg ccatcacctg caacgaccag
    36061 aatcgatgct tcatcaacaa acttcatttt attctccgta aatcattcgc ctgcgcgggg
    36121 ttgcgatacc accgttgtat gcttacgtaa tccgccccaa atacgatgac caatggcgga
    36181 atacatcgcg cccgcaacca cgacaaacgc accgagataa cctaaaaggt ttaacatcgg
    36241 tctggcgaag aaatcgggcc aggccagtga taaaagatct gaaaaaaaca gcgtaaacag
    36301 tggggtgagc gtgatgatcg cgctcacctg agctgcctgc cagcgagcca tcgcttccgc
    36361 caaggcgcca tatcctacca aggtattcag tccgcaaaaa attaaacatg cgagctgcca
    36421 gtggctaagc tgcgctatca ctccaggctt tgccagaggg aagagcgcga ttgtacataa
    36481 agtgtacagt aaaaacagga tctgcggtga ggccagccga cgcaataaaa ccttttgcgc
    36541 cacgccataa ctcacccaaa ccgtcgccgc accgacccca aagataactc cccaggtgta
    36601 atcggtgagc tttgtaaata tctcgaccag actggtgtta aaaaacatca ccagaccgct
    36661 caggagcatc aatgccccta caacctgagt gctgcgcatt ttctctttca ggataaatac
    36721 gctggcaacc atcatgccaa ctggcgagag ttgcccaatc acctgcgaag cggtcggact
    36781 caggtattgc aaggatgagc tgaacaggat gaagttccca aacagcccgg cggtcgccac
    36841 tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt aatggcggca acctcttctt
    36901 caccgcaaga atggcaccaa ggccaatact cgccatcaag aaacggtaaa acacgattgt
    36961 cggaggttcc atcacctcca gcacctgctt cattgcgatt ggcaacgccc cccagcaaat
    37021 tgctgtggta agcgccaaaa gaatgccaat gcctgcctgc tgcttcatac cgtattccct
    37081 gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt tttaccggtt atcgaatgta
    37141 aaaagccccg caacgtgttg cggggctttc atccgttacc gggacgcgaa aaacttattc
    37201 agcttcgatg ctgataaatt tacggttttt cgggccttta acttcgaatt tcactttacc
    37261 gtctgcttta gcaaacagag tgtggtcacg accgcaacct acgttagcgc cagcgtggaa
    37321 tttggtacca cgttgacgaa cgatgatgct acccgccaga acggattcgc caccgaaacg
    37381 cttaacgccc aggcgtttag cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt
    37441 tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc tgatgccagt aattttcaca
    37501 tcagtgaacc actgacgatg gccctgctgc ttacgatagt gtttacgacg acgaaactta
    37561 acgattttaa ctttctcgcc acgaccgtga gcaacaactt cagctttgat tacgccgcca
    37621 tcaacgaaag gaacgccgat tttgacttct tcaccgtttg cgatcatcag cacttcagcg
    37681 aactcaacag tttcgccagt tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg
    37741 cttactcggt gttgtttacc accactttgg aaaaccgcgt acatataaaa ctccgcttcc
    37801 gcgcacacct tttcaatgat tcaaagtgcg ctataaatat tcacaatagg gcgcgaatat
    37861 tacgcaaaac gcacgccttt gacaagtgct acagtcaata cacgaagaaa aaaaacacaa
    37921 cttgtacggt aacgtttatc tgtgccattt tttcagtaca attaccctat attcctaacc
    37981 ataaacccta agttgccttt gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg
    38041 cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg
    38101 caatccttga gcagcttaat tccgacgtcc aactgatcaa tcagttaggc tattacatcg
    38161 tcagcggcgg cggtaaacgt attcgtccga tgattgctgt actggctgca cgagctgttg
    38221 gctatgaggg aaatgcgcat gtcaccattg ctgccctgat cgagtttatc cacacggcga
    38281 ctctgctaca cgacgacgtt gtggatgaat cagatatgcg caggggtaaa gctaccgcca
    38341 acgccgcatt tggcaatgcc gccagcgtgc tggtaggcga ttttatttat acccgcgcgt
    38401 tccagatgat gaccagcctc ggttcactca aagtgctgga agtcatgtca gaagctgtaa
    38461 acgtcatcgc agaaggtgaa gttctgcaac tgatgaacgt taacgatccg gacatcactg
    38521 aagaaaacta catgcgcgtt atctatagca aaaccgcgcg tctgtttgag gctgccgcgc
    38581 agtgttccgg gattctggct ggctgtacgc cggaggagga gaaaggcctg caggattatg
    38641 ggcgctatct cggcactgct ttccagttga tcgacgattt actcgattac aatgccgatg
    38701 gcgaacagtt aggtaaaaac gtcggcgacg atctgaacga aggtaaaccg acgctgccgc
    38761 tgctgcatgc gatgcatcat ggcacaccag aacaggcaca gatgatccgt accgccatcg
    38821 aacaaggtaa cggtcgccat cttctggaac cggttctgga agcaatgaac gcttgtggat
    38881 ctcttgaatg gacgcgtcag cgcgccgagg aagaagcaga caaagccatc gcagcgttac
    38941 aggtgctccc ggacacccct tggcgagaag cactcatcgg cctcgcgcac atcgctgttc
    39001 aacgcgatcg ttaatcccct cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat
    39061 tccataaaaa cactaattca gcgctataac acctgaaaaa aagggcgatg aatctttata
    39121 tattctgaat attcacactc tttacaggaa ctttttagcg caataggcca tcaggagtat
    39181 agtgatgccc gacagaagaa gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa
    39241 gatggaaagt aatttcattg actggcatcc cgctgacatc attgcaggtt tgcgcaagaa
    39301 aggaacgtca atggcggcgg aatctcgcag aaatggtttg agttcctcaa cgctggcaaa
    39361 cgcattatcg cgcccatggc cgaaaggaga gatgattatt gcgaaagccc tgggaactga
    39421 cccctgggtt atctggccat cacgctacca tgatccgcag acccatgagt ttatcgacag
    39481 aacgcagttg atgcgtagct acactaaacc gaaaaaatga gtggtctggc ggtagccccg
    39541 cgaacggggc tgccagctct cagacgatta ctcgcctttc acacgctcaa tatttgcacc
    39601 taaagcgcgc agtttgtctt caatgcgttc gtagccacga tcgatgtgat aaatacgatc
    39661 aaccaccgtc gtcccttccg caatacagcc agccagcacc aggcttgctg atgcacgcag
    39721 atcggttgcc ataacctgtg caccagaaag tttttcaaca ccatgacaaa taacggtatt
    39781 gctttcgatt tcggcgtgcg cgcccatacg gctcagctct ggcacatgca taaagcggtt
    39841 ttcaaagacc gtttcggtga tgaacccggt cccttcagcc accaggttca acagcgtgaa
    39901 ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt
    39961 cggacgtttg ccatgcatat ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc
    40021 agcatcacgc agtttcgcca gcacggcgtc cagagtatct ggctgcgcgt tacggcagat
    40081 aattttgcca cgagagatcg ccgccgccac caggaaagta ccggtttcga tacgatccgg
    40141 cagcacgcga tagacaccgc cgcctaaacg ttccacacct tcgatgacga tacgatcggt
    40201 gccctgaccg ctaattttcg cacccagcgt aatcaggaag ttcgcggtat cgacgatttc
    40261 cggttcacgc gctgcgtttt caataatcgt ggtgccttcc gccagggttg cagcacacat
    40321 gatggtcacc gttgcgccaa cgctgacttt atccatcacg atatgtgcgc ctttcagacg
    40381 accatcgacg gaagctttaa cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc
    40441 gaggccagaa atgtgtagat caaccggacg agcaccgatc gtacaaccgc caggtagtga
    40501 aacttgcccc tgaccaaagc gcgctaccag cggccccagc gcccagatag aagcacgcat
    40561 ggttttaacc agatcgtaag gtgcgcagaa tacattaacg tcgcgggcat caatatgcac
    40621 agaaccatta cgttctactt tcgcacccag ctggcttagc agtttcattg atgtatcgac
    40681 gtctttcagt ttcgggacgt tctggatctc taccggttct tccgccagta gtgcggcaaa
    40741 aagaataggc agagcagcat ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt
    40801 tggcccctga acacgaaatt tatccattta gtttgttctc agttaacaat tcatatccgc
    40861 taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc
    40921 aggggtatac gctttgatcg acacagcatg aatgcggtta tccgcaatat attccatcag
    40981 cgggccatag accgtctgct gttttttaac ccgactcata ccgtcaaaca actcacccac
    41041 ggcaataacc tgaaagtgac tgccatcgcc ggaaacgtgg acttcctgga gggagagagc
    41101 gttcatcaac acgctctgaa tttcattatt ttccatggga tcttcaatca tcagttaata
    41161 aaccagcgaa acatcttaga gcaaagttgc gctggcataa ataagcaaaa agcctcgctg
    41221 ataaatcaga caaggctcga cttgcaggca ggtttgccgg acaggcggtt aacgccatat
    41281 ccggcctgaa aaaatttaac gaggcagaac atcagcaggc aaattataca atttcgcgag
    41341 ggtatacact ttgtcgttta ccccctgaag cgtcacgttg ttgccctgct ttttcgccag
    41401 atcgataaga tggagcagca gtgccagtcc ccccgtatcc acgcgggaca cacggctaag
    41461 atcgatgcag gtaatcccct tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac
    41521 gtcctgatcc agctctccgg ataacgccag cgtgtcaccc gtctgcatcc agctcagtga
    41581 ctcgctcatt attttttctc ttccagagtg attttctgtt gagaaatcga tttcagttgc
    41641 gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc cccactcgtt ttgtttggtg
    41701 gtgatcatac tgacgccttc agcaatcatg tcgtaagctt gccagtttcc cgtctgggag
    41761 tttttacgcc actggaagtc cagacgcacc ggcggacggc cattcgggtc aataatggta
    41821 acgcgaatag gcacaatggt tttatcgccc agcggctgtt ctggcgcaat ctgataggtt
    41881 tgaccgtgat acatcgccag cgcctgaccg taagcctgct tcaggtactc acggaaagcg
    41941 gcaaagtagg cttcacgttg agcaggggtc gcactcttgt aatactggcc cagcaccagc
    42001 gcaccggcgt atttcacctg tacgtatggc agcagttcct gatcaacaat ggtacgcaga
    42061 taatccgggt tggcccgaat ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc
    42121 gccgcctcgt ccatcagctt ataaggattg gtctggtctg ccgcggttgc cgcactcaga
    42181 ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa acatgcgtcg gttctcctga
    42241 aattatttcg ttgtacccac aggttcagtg gtttcattat tacctggcgc agcagctggc
    42301 gcatcgccac tattcttatt gtcatcgcct ttactaccgt aaaggaactg accaatgaga
    42361 tcttccagca ccatcgcaga cttggtgtcc tgaattgtat cgccatcctt caggatagca
    42421 gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca gatattgttc tcccagcagg
    42481 ccggaagtac gaatgctcag cgaactggta tcaggaatgt ggttataacg ttgttcaatt
    42541 tccagcgtta cgcgcggcag ataggttttc gggtccagcg taatatccgc cacccggccc
    42601 acaacaacac caccaatgct gaccggagag cgcgctttca ggccgccaat gttatcgaac
    42661 gtcgcataaa gcgtgtaggt cgattcagta cgtatggacg tcacgttcgc cgccttcagg
    42721 caaacaaaca gcgccgccag cagtgctgct aataaaaaga tacccaccca aatttcattt
    42781 tttttcgttt gcatgaactc aattcccaaa catcaatgcg gtcagcacaa aatccagccc
    42841 cagaacagcc agagacgagt ggacaacggt gcgagtggtt gcccggctaa tcccggcaga
    42901 cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac
    42961 cacgctctta atcagacagt tgaccagatc catacgccag tcgacggcat tttgcattgc
    43021 cgaccagaag aacccgctat caatgccttt ccaactgacg ccgaccagcg atccgcccca
    43081 gatccccacg gcgacgaaga taaccgtcaa cagtggtaat gaaataaccc cagcccagaa
    43141 acggggagaa ataacccgac gcagcggatc caccgccatc atctccatac tggagagttg
    43201 ctctgtagcg cgcatcaggc cgatttctgc ggttaacgcc gaaccagcac gcccggcaaa
    43261 caacagcgcg gcaaccaccg gccccagttc acgcagtagc gataacgcca ccagcatacc
    43321 cagactggtt tccgcgctat aagtggtcag aaccagataa ccttgcagcc ccaacaccat
    43381 tccgatgaac acgccagaaa ctacaataat cagcatcgac aggacgccga cattatagag
    43441 ctggcgcacc agcagcggcg catgtttgcg aaattccggt ttgccgacca gcgcattgaa
    43501 taacattaac ccggcccgcc cgaacgttct cagggtttta atccctttat gtccgagcga
    43561 cgccagcgca tttaacagca tgagtggctt aactccctgg taaaagatca gcgtgataat
    43621 cgccggcagg atagcggaac ggaacaggcc cgtcagctat cccgtccaga aactgacgta
    43681 cgcgcggatc aggattcgtc tgcaacgcct gggcactgcc atgagcgaca atttttttgt
    43741 ccgccaggat ccaggcgtga tccgcaatac ttaacacttc cggcacatcg tgagaaacca
    43801 ccacacaagt cacgcccagc gcgctgttca gctcagaaat cagcttcacc agtacgccca
    43861 tggtgatggg atcttgccca acaaaaggtt catcaaacat gatgagatcc ggctccagcg
    43921 caatcgcacg cgccagcgct gcacgccgcg ccatcccacc ggaaagttca gaaggcatta
    43981 gtttagccgc tccacgcagc cccacggcct ccagcttcat catcaccgta ctatgcaaca
    44041 atggcgcggg aagttgggta tgttcgcgca gtggataggc gacgttgtca aatacgttca
    44101 tatcagtgaa caacgccccg gactgaaata acatgctcat ccgtttacgc actgtataca
    44161 ggcgcgaacg agacatcgcc ggaatattct caccatcgaa aaggatctca ccatgatctg
    44221 gtgcgatttg cccgccaatc agacggagta gcgtcgtttt accgatgccc gatggcccca
    44281 tgatcgccgt gatcttccct cgcggcacgg tcagggaaat attatcgaag atgcagcgat
    44341 tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt cgccacagac tgctccataa
    44401 ttcacccttc gtcttgcgtt gattttctaa gcatggcgct caatttaacc ttgaacccaa
    44461 catatttaca gaatattacc cgccgtggtt agcgaaagct ggcatttgtt ttacttttta
    44521 gccgcataaa gtcaaaatta agaatccgtt acggctttct gaaaatcttc agcggaccgg
    44581 cgagtatacc tgaagaaagg acgttagatg cttttagcta cggcactgtt aattgttggt
    44641 ttacttttgg tcgtttacag tgccgaccgc ctggtttttg ccgcgtctat tctttgccga
    44701 acctttggca tcccgccgct gatcatcggc atgacggtgg tcagtattgg tacatcgtta
    44761 ccagaaatca tcgtctcgct tgccgcgtct ctgcacgaac aacgcgattt agccgttggt
    44821 acagccctcg gctcaaacat tatcaatata ttgctgatcc tcggtctggc tgcgctggtt
    44881 cgtcctttta ccgtccattc cgatgttctg cgccgtgaat tacccttaat gttgttggtc
    44941 agcgtggtgg ccggttccgt actctatgac ggacaactta gtcgaagcga tggtatcttt
    45001 ctcctctttc tggctgtgct atggctgctg ttcattgtta aacttgcacg tcaggggact
    45061 gacagcctga ccagagagca gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg
    45121 tttttatggc tcggcattgc gcttatcatc atgccagtgg ccacgcggat ggtggttgat
    45181 aacgccacgg tgctggcgaa ttactttgcc atcagcgagt tgacgatggg tctgacggca
    45241 attgctatcg gaaccagtct gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt
    45301 gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg
    45361 ttgggtttac ccgcgctgat aacgccagga gagattgatc cactggcgta cagtcgtgac
    45421 tacagcgtga tgttgctggt gagcattatt tttgcgttgc tgtgctggcg gcgctccccg
    45481 caaccgggcc gtggtgtagg ggtattatta actggcggat ttatcatatg gctggcgatg
    45541 ttgtactggt tatcgccaat actcgttgaa taactggaaa cgcattatgt cgcacgtaga
    45601 gttacaaccg ggttttgact ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga
    45661 atgcctggcg gagcttgatc aatacatcaa tcagaatttc acgcttgcct gtgaaaagat
    45721 gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg
    45781 caaaatggcc gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc atcctagtga
    45841 agccgcgcat ggtgatttag gcatggtcac cccacaggat gtggtgattg ctatctctaa
    45901 ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg cttaagcgtc ttcacgtacc
    45961 gttaatctgc atcaccggtc gcccggagag cagcatggcg cgcgccgcag atgtgcatct
    46021 gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg gcaccgacca gcagcaccac
    46081 cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac
    46141 tgctgaagat tttgcgctct cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg
    46201 cgtaaacgat attatgcata cgggcgatga gatcccgcat gttaagaaaa cggccagtct
    46261 gcgtgacgca ttgctggaag ttacccgcaa aaatcttggt atgactgtca tttgcgatga
    46321 caatatgatg attgaaggca tctttaccga cggtgattta cgccgtgtct tcgatatggg
    46381 cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg
    46441 ccctggcatt ctggccgttg aggcactgaa cttaatgcag tcccgccata tcacctccgt
    46501 gatggttgcc gatggcgacc atttactcgg tgtgttacat atgcatgatt tactgcgtgc
    46561 aggcgtagtg taaagattca aggataaaca acaatgagca aagcaggtgc gtcgcttgcg
    46621 acctgttacg gccctgtcag cgccgacgtt atggcaaaag cagagaacat tcgtctgctg
    46681 atcctcgacg ttgatggcgt actgtcagat ggcctgattt atatgggcaa taacggcgaa
    46741 gagctgaaag cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct cacctctgat
    46801 attgaagtcg ctatcattac cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca
    46861 ttggggatca ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt tagcgatttg
    46921 ctggaaaaat tggcgattgc cccggaaaat gtggcttatg ttggcgatga tctcatcgac
    46981 tgcccggtaa tggaaaaagt gggtttaagc gtcgccgtgg ccgatgccca tccactgttg
    47041 atcccgcgcg ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt
    47101 tgcgacttat tactcctggc gcagggcaaa ctggatgaag ccaaagggca atcgatatga
    47161 gtaaagccag acgttgggtt atcattgtgc tatcactggc ggttctggtg atgatcggca
    47221 ttaatatggc cgaaaaagac gataccgccc aggtggtcgt caacaacaat gatcccacct
    47281 ataaaagcga gcatacggac acgctcgtct ataacccaga aggggcacta agctatcgat
    47341 tgattgctca acacgttgaa tattattccg atcaggccgt ttcgtggttt acgcagccgg
    47401 tacttaccac gtttgataag gataaaatcc cgacatggtc cgtaaaagca gataaagcca
    47461 agctgaccaa tgaccggatg ctctatttat atggacacgt tgaagtcaac gcacttgtgc
    47521 cagactctca acttcgcaga atcacgacgg ataacgcgca gatcaatctg gtgacgcagg
    47581 atgttacctc tgaagacctc gtcacgttat acggaacaac atttaactcc agcggtctga
    47641 aaatgcgcgg caacttacgc agcaagaacg ccgagctgat tgaaaaggtt agaacatcct
    47701 atgaaattca aaacaaacaa actcagcctt aatcttgtgc ttgccagctc acttctggcc
    47761 gccagcattc cggcatttgc cgtaaccgga gacactgatc agccgatcca cattgaatcg
    47821 gaccagcaat ctcttgatat gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc
    47881 acccagggca ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa
    47941 caaggtaaag aagtgattga cggatacggt aaaccggcaa cgttctacca gatgcaggac
    48001 aacggtaaac ccgttgaagg tcacgcttcc cagatgcact acgaactggc aaaagatttt
    48061 gtcgttctga cgggtaatgc ttatctgcag caggtcgata gcaacattaa gggcgataag
    48121 atcacttacc tggtgaaaga gcagaaaatg caggctttca gcgacaaagg caagcgcgta
    48181 accaccgttc tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca gaccccggca
    48241 cagaagaagg gtaattaatt cgttatggca acattaactg caaagaacct tgcaaaagcc
    48301 tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg ggaaattgtc
    48361 ggtctgctgg ggccaaacgg tgccggtaag accaccactt tctacatggt tgtaggcatt
    48421 gtgccgcgcg atgcgggcaa catcattatt gatgatgacg atatcagtct gctgcctctg
    48481 catgcacgcg cgcgccgcgg tatcggctat ctgccacagg aagcctccat tttccgtcgc
    48541 ctcagcgttt acgataacct gatggcggta ctgcaaattc gtgacgactt gtctgctgaa
    48601 caacgtgaag accgcgcgaa cgagctgatg gaagagtttc acattgagca cctgcgtgac
    48661 agcatggggc agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg
    48721 gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg ccggggttga cccgatctcg
    48781 gttatcgata ttaaacgcat cattgaacac ctgcgcgaca gcggcctggg cgtgctgatc
    48841 actgaccaca acgtgcgtga aacactggcg gtttgtgaac gcgcttatat cgtcagtcag
    48901 gggcatttga tcgcccacgg cacgcctaca gaaatcttac aagacgaaca cgttaagcgt
    48961 gtataccttg gggaagactt cagactctga tagggtagaa gtttgcgacg ttttagcagg
    49021 agagtacgat tctgaacatg aagcaaggtt tgcaactcag gcttagccaa caactggcga
    49081 tgacgccaca gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc
    49141 aggagctaca gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac actcatgaag
    49201 aaatcgacac ccgcgaaacg caagacagtg aaacgctgga caccgccgac gcgctcgaac
    49261 aaaaagagat gccggaagag ctgccgctcg atgccagttg ggacaccatt tacaccgctg
    49321 gtacaccatc cggcaccagc ggtgactaca ttgacgacga gctgccggtc taccagggcg
    49381 aaacgacgca gaccttgcag gattacctga tgtggcaggt cgagctgaca ccgttttccg
    49441 acactgaccg cgctattgct acctctatcg tcgatgccgt tgatgaaacc ggttatctga
    49501 ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga agagatcgac atcgacgagg
    49561 ttgaagccgt ccttaagcgg atccaacggt ttgatccggt cggtgtagcg gcaaaagatc
    49621 tgcgtgactg cctgctaatc caactctccc aattcgataa gaccacgccg tggctggaag
    49681 aggccagatt gatcattagc gatcatctcg atctgttagc caatcacgac ttccgcactt
    49741 taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat ctgatccagt
    49801 cgctcgatcc gcgccccggg cagtcgatcc agactggcga acctgagtat gtcattccag
    49861 atgtgctggt gcgtaagcat aacggtcact ggacggtaga actcaacagt gacagcattc
    49921 cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg
    49981 acagccagtt tatccgcagc aatctgcagg atgccaaatg gttgatcaag agtctggaaa
    50041 gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag caagccttct
    50101 ttgagcaagg tgaagaatat atgaaaccga tggtactggc cgatatcgcc caggctgtcg
    50161 aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa atacctgcat agtccacgag
    50221 gcatttttga actgaagtat ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag
    50281 cttcctccac ggcgattcgt gcactggtga agaaattaat cgcggcggaa aacccagcga
    50341 aaccgttgag cgacagcaag ttaacctctt tgctgtcgga acaaggtatc atggtggcac
    50401 gccgcactgt tgcgaagtac cgagagtctt tatccattcc gccgtcaaac cagcgtaaac
    50461 agctcgtttg acccaaccga taaggaagac actatgcagc tcaacattac cggaaataac
    50521 gtcgagatca ccgaggcact gcgcgaattt gttacagcta aatttgccaa acttgagcaa
    50581 tattttgacc gaatcaacca ggtctatgtt gttctgaaag tggagaaagt cacccacacc
    50641 tcagatgcaa cactgcatgt aaacggtggt gaaattcatg ccagcgcgga aggtcaggac
    50701 atgtacgctg ccattgatgg cttaattgat aagctggcac gccagttgac caaacataaa
    50761 gataaactga aacaacacta attgtccggg caattagcat gtgcatggcg gcctgttgtg
    50821 cggcacaacg ggccatttgt acggttaatg ctccgagcct gttccactgt ttgagtgggc
    50881 aggttcttag gtgaaattat gacaaataat gatacaactc tacagcttag cagtgttctt
    50941 aacagggaat gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc
    51001 atcagcgagc tggcggcgaa acaacttagc ctaccgcctc aggtggtttt tgaagctatc
    51061 ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg gtattgccat tccgcatggc
    51121 aaacttgaag aagatactct gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata
    51181 gctttcgatg ccatcgacaa ccaaccggtg gacttacttt ttgccctgct ggtgccggca
    51241 gaccaaacta aaacgcacct gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa
    51301 accatctgcc gccgtttacg cgcagcccag agcgatgaag agctgtatca aatcattacg
    51361 gataccgaag gtactccgga tgaagcgtag ttattcggta atgtctcttt tagacgttgt
    51421 gaggagaaac agtacatggt actgatgatc gtcagcggac gttcaggttc aggtaaatct
    51481 gtcgccctgc gtgcgctgga agatatgggt ttttactgcg tggataacct tcccgtagtg
    51541 ttgttacccg atctggctcg aactctggcc gatcgcgaga tttctgccgc cgtcagcatt
    51601 gatgttcgta atatgccgga gtcaccagaa atattcgaac aggcgatgag taacctgcct
    51661 gacgctttct caccgcaact actgttcctg gatgccgacc gtaatacctt aattcgtcgt
    51721 tacagtgaca cgcgccgact gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct
    51781 atcgacaaag aaagcgattt gctggagcct ctgcgttcgc gagcggatct gattgtcgat
    51841 acctcagaaa tgtccgttca cgagctggct gaaatgctgc gtacccgtct gctgggtaaa
    51901 cgtgaacgcg aactgaccat ggtctttgag tctttcggct tcaaacacgg tatccctatc
    51961 gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg
    52021 cgtccaatga caggtcttga taaacctgtc gccgcgttcc tcgaccgcca cacagaagta
    52081 cacaatttta tctaccagac gcgaagctat cttgagctat ggttacctat gctggaaacc
    52141 aacaaccgta gctacctgac ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg
    52201 tatattgcag agcaactggc agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc
    52261 catcgtacgc tggaaaaacg taaaccatga ccgtcaagca aactgttgaa atcacaaaca
    52321 agctgggcat gcatgcccgg cctgcaatga agctgtttga attaatgcag ggttttgacg
    52381 ctgaagtgct gttacgtaat gacgaaggca ccgaggctga agccaacagc gttattgcgc
    52441 tgctgatgtt ggattctgcc aaaggacggc agattgaagt tgaagcgacc ggtccacagg
    52501 aagaggaagc actggccgcc gttatcgccc tctttaattc tggttttgat gaagattaat
    52561 cttcatcact ttgacataca actcccttca agctcccccg acaataagaa aatcacgtac
    52621 tgaaatcgtt ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa
    52681 ccttaagaaa accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg
    52741 agatcgtcag aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct
    52801 gggcaccgga agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa
    52861 gattgtctac catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta
    52921 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    52981 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    53041 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    53101 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    53161 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    53221 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    53281 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    53341 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    53401 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    53461 aatccacgcc tgacggctac gcacgtagcc gtcaggcgtg gagactttga aacgaagtgg
    53521 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    53581 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    53641 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    53701 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc
    53761 cgtctgctga gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc
    53821 cagggctttc tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc
    53881 tgcggcaatc accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga
    53941 atgtgccacg taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac
    54001 cgctgagaag ggaacaggcg caacgctaaa caacgcgatg cccccgcccc agaaaacggc
    54061 gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct
    54121 tttactcatt cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct
    54181 tctgcaatgt tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca
    54241 accacgatat catcgacagg acacggcaca tgctccgcgc ccatctcttc cagtacttct
    54301 gcggtatcga tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc
    54361 atcgccgggg caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc
    54421 agcgccttta attcacggtc aacggtgcat tcgctaccaa gactggcaaa attgcttaaa
    54481 ttcttcgccg cgccaacccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc
    54541 tgggccagag gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacattgcgc
    54601 gtttccgtca tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt
    54661 gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca
    54721 tgaatttcag aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt
    54781 gtgatcatcc tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca
    54841 tcacacattt aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc
    54901 ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag
    54961 atttccctgg tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt
    55021 ggcttttgcc acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc
    55081 ttcaatccat tcaccgacgt tatcttccca ggcgggaagg tcaggagact gaatttgctg
    55141 acccagctgt tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc
    55201 ttcctcaaca atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata
    55261 gcccggcatc atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag
    55321 ttcgagatac tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc
    55381 tgtcgtcacc gtactcttct catcatcctg ggtatcccag aatttcttga tcatcgcggt
    55441 tagcgccgga accgaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc
    55501 ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg
    55561 ataacgcttc gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc
    55621 cagcaacacc aggtcgtatt cgcccggttt aaacatctcc agcgccgcct tgccggtcat
    55681 ggcgacatca acgctgttac ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag
    55741 ttcaatgtct tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa
    55801 cgcatcatcg acctcttctg ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc
    55861 tttgccctgt tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga
    55921 aacggccaga ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg
    55981 gtaatacatg gcgaagattt tatccagctc atcctgcgga atgccgatac cagaatcttc
    56041 cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg
    56101 ctgggtgaat ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc
    56161 gtcggtaatg acctgatgcg gtaatggcag cgtaggctcc aggttaaagc gcaatccttt
    56221 ttgttgcgcc tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac
    56281 cggctgatta tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt
    56341 aaagatattc cccagcgtga cggccgaaac atggatagtt ttgagatatt tttcctgctc
    56401 ggcggtgagt tcggtatcca gcagaatgcg gctcaggccg acgataccgt tcagcggtgt
    56461 acgcaattcg tgactgatgg tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag
    56521 cgcatcctga taccgcttac gctcggtaat gtcgcgacca aagcccatca aaccgtgacg
    56581 tttacccacg cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc
    56641 cgggtaatcc agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc
    56701 ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcagatgaac
    56761 cagttgtttt tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc
    56821 tttatcttcg ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa
    56881 tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag
    56941 ttggccgaag gtttcctgca gttctgcttc cgctttttca cgaacggcaa tttcctgatt
    57001 tagctgggca atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc
    57061 ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac
    57121 caccgataga aagtagaccg cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat
    57181 aacatcaatg ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat
    57241 ggcaagaacg acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat
    57301 catcaggtca acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca
    57361 cgacaacctg ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat
    57421 gcggaattcc cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat
    57481 ccctgtacgc catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt
    57541 tcgcaccaca gcggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag
    57601 atcacttccg gtggcgtatg gcgaatcatt tctccggcag tgagcgtata atcctccagt
    57661 tcaataccgt tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata
    57721 tgcagcggat gcagctttat gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc
    57781 aaacattcag cctgcccttc gccaggcagg ccgacaatta agtgggaaca caccttcagc
    57841 ccgcgctgac gtgccagctg ggttgtacgc tgataacagg caaaatcatg gccgcggttg
    57901 atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca gctccagcca cacttcgtaa
    57961 ccctggtcct tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgg
    58021 gtaccaacac acaaaccgac aatattggcc tggctcaccg cctgctgata catagaacgc
    58081 agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa agtaggccag atagcgttta
    58141 gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg caatggaacg atgctgctgc
    58201 gcttcatcgg caaacgaggc aacattacag aatgtgcagc caccacgccc gatggtaccg
    58261 tcacggttag ggcagctaaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa
    58321 cgacgggtga gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag
    58381 accgcgcctt gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctag
    58441 atcaatcgtc ccgccctgct tttatcaact gcataatcaa tcaaaattac cgaaatttca
    58501 tgcataatca cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg
    58561 attttaggca aaaacagtag catgaaacgt cattaccaat taaggcagta taaaatgctg
    58621 gttttctcgt cagttcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt
    58681 gactgtttct ctaacgactc tccttttagc cttaaagata aaatctattt taatttcagc
    58741 catttaataa agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac
    58801 ctgtatcagc tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg
    58861 ctcatcatat ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag
    58921 gacaacaagg gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct
    58981 ttctgtgccc gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca
    59041 cgatatgttg tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca
    59101 catagaaggc gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat
    59161 gcagcaccgt ggcgcgattc tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt
    59221 acaaaaaccg gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa
    59281 aaactacgct gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg
    59341 ccgcatcgtt gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt
    59401 ccccactaac gaaggcgtgc tgggtgaaat cgccctctcc tctctgccac gcattgagca
    59461 aatttttgtg aacgccccgg caggctggcg tccacgcgat atggagcgcc gtctgtttat
    59521 cgcccgccgc cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct
    59581 gtcgaatctg gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta
    59641 tctggatctt gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc
    59701 cactaacacc gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg
    59761 tgaaatcaac accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca
    59821 gacgccgctg atccctgacc tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga
    59881 ctccagttcc atggataaca tgctggaact gctgctggca ggcgggatgg atatcatccg
    59941 cgccatgcgc ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact
    60001 gcgtgccttc tttgacttta actccatgca tatggagccg tgggatggtc cggcgggcat
    60061 cgtgatgtcc gatggtcgct tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc
    60121 acgctacgtc attaccaaag acaagctcat cacctgcgcc tctgaagtcg gtatctggga
    60181 ttaccagcct gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat
    60241 cgacactcgc agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg
    60301 ccacccttat aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct
    60361 gcccgatgaa gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa
    60421 acagtttaac tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg
    60481 tcaggaagcg gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc
    60541 gcgcattatt tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga
    60601 cccgctgcgt gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt
    60661 cttttgcgaa gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta
    60721 ctccgatttc aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga
    60781 tatcaccttt gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa
    60841 agccgaaaaa atggtacgta gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc
    60901 taaagatcgc ctgccggttc cggctccgat ggcggttggc gcgatccaga cccgtctggt
    60961 cgatcaaagc ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc
    61021 gcaccacttc gccgtgttgc tgggtttcgg cgcgacggct atttatccgt accttgccta
    61081 tgaaacgctg ggccgcctgg tagacaccca cgcgattgcc aaagattatc gtaccgtgat
    61141 gctcaactac cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat
    61201 ctccaccatc gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga
    61261 tgtagtgggc ctgtgcttcc agggcgcggt cagccgcatt ggtggggcaa gctttgaaga
    61321 cttccagcag gatctgctga atctgtcgaa acgtgcctgg ctggcgcgca agccgattag
    61381 ccagggcggc ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt
    61441 ggtgcgcacg ctgcaacagg cggtacaaag cggcgagtac agcgactatc aggaatacgc
    61501 gaagctggtt aatgagcgtc cgacaaccac gctgcgcgat ctgctggcaa ttacgccggg
    61561 tgaaaacgcg gtcaacattg ctgatgttga accggcaagc gaactgttta aacgctttga
    61621 taccgccgcg atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc
    61681 gatgaatagc atcggcggta actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta
    61741 tggcactaac aaagtgtcgc gcatcaagca ggtagcttct ggtcgcttcg gcgttactcc
    61801 ggcgtatctg gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg
    61861 cgaaggcggt cagttgccgg gcgacaaagt cactccttac atcgccaaac tgcgctattc
    61921 ggtgcccgga gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgaaga
    61981 cttagcgcag cttattttcg acctcaagca ggttaacccg aaagcgatga tctccgttaa
    62041 gctggtgtcc gaaccaggcg ttggcaccat cgcgactggc gtggcgaaag cttatgcgga
    62101 cttgatcacc atcgcgggct atgacggcgg caccggcgca agcccgctgt cgtcagtgaa
    62161 atatgcaggc tgcccgtggg aactcggtct ggtggaaacc caacaggcgc tggttgccaa
    62221 cggcttgcgt cataaaattc gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat
    62281 catcaaagcg gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtggc
    62341 gctcggctgt aaatatctgc gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac
    62401 tcaggatgac aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgactaatta
    62461 ctttgagttt atcgcccgtg aaacccgcga gctgatggca cagctgggag taacgcgtct
    62521 ggtggatctg attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca
    62581 gcagaaactg gcgctgtcga agctgctgga gactgccgag ccacatgcag gtaagtcact
    62641 ctactgcact gaaaacaacc cgccgtttga taacggccta ctgaacgcgc agttgctgca
    62701 acaggcgaaa ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa
    62761 caccgaccgt tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca
    62821 ggggctggca gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg
    62881 tgtgtggaac gcgggcggcg tggaactata cctgaccggg gatgccaacg actatgtcgg
    62941 taaaggcatg gcgggcggct taatcgccat tcatcctccg gttggttccg ccttccgcag
    63001 ccatgaagca agcattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta
    63061 tgccgcaggc cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt
    63121 agaaggcatt ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg
    63181 taaaaccggc gttaacttcg gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga
    63241 aagcggtgat ttccgcaaac gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga
    63301 tctggcgatc catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcataccgg
    63361 ttctcagcgc ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct
    63421 ggttaaaccg aagtccagtg atgtaaaagc actgctgggt caccgtagtc gtagcgcagc
    63481 agagttgcgc gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt
    63541 tatcgacctg cagcgcgttg atccgccaaa gaaaccgctg aagatccgta aaattgagtt
    63601 tgttgaaatt tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgtct
    63661 gtcgtgcggc aacccatact gcgagtggaa atgcccggta cacaactaca tcccaaactg
    63721 gctgaagctc gccaacgagg ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa
    63781 cacactgccg gaagtatgcg gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg
    63841 caccctgaac gatgagttcg gtgcggtgac catcggcaac attgagcgtt atatcaatga
    63901 taaagcgttc gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa
    63961 agtggcgatt atcggtgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa
    64021 cggcgtaaaa gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg
    64081 tattccggcc ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg
    64141 catgggtatt gaattcaaac tcaataccga agtgggccgc gacgtacagc tggacgatct
    64201 gctgagtgat tacgatgccg tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg
    64261 gctggaaaac gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac
    64321 caaacagtta atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa
    64381 acgcgtggta gtacttggcg gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg
    64441 ccagggggca aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc
    64501 ccgccgcgaa gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc
    64561 actggggatt gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga
    64621 aatgggcgaa ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca
    64681 tatcgttccg gcagatgcgg tgatcatggc gtttggtttc cgtccacaca acatggaatg
    64741 gctggcaaaa cacagcgtcg agctggattc acaaggccgc atcatcgctc cggaaggcaa
    64801 cgacaacgct ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg
    64861 ttccgatctg gtcgttaccg ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa
    64921 ctggctggaa gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa
    64981 attctccttt ctttctggaa atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct
    65041 catctggatt gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg
    65101 ccatataaag ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca
    65161 aatccacgac ggcattattc atctggtcga tagcgcccga acggaaacgg tacgtagcgt
    65221 taacgcgctg atgaccgcga cgtactggga aattggccga cggattgtcg aatttgaaca
    65281 aggtggtgag gccagagcag cttatggtgc acagttgatt gatcggctat cgaaggattt
    65341 aagtcgacgg tataaacgtg gggtctctgc aaaaaactta cgccaaatga ggctttttta
    65401 cctctttttt caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg
    65461 aattccgcag acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc
    65521 caaattgaga ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt
    65581 tgtatattca tgcaaataaa cgaagg
//
LOCUS       DADLTF010000006        57156 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.006, whole genome shotgun sequence.
ACCESSION   DADLTF010000006 DADLTF010000000
VERSION     DADLTF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.006"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JN749_000402"
     CDS             <1..848
                     /locus_tag="JN749_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7366931.1"
                     /translation="VLVVFVGILLLMAFVVLPIAWQQGIYLIRDMPGMLNKLSDFAAT
                     LPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLLTIAVYLVLVPLMVF
                     FLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVLEMIVVGIATWLGFL
                     LFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGTEFWSCFAVYLIIQA
                     LDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPLATLIKAVIHAWPDG
                     QIAQE"
     gene            complement(886..1734)
                     /locus_tag="JN749_000403"
                     /pseudo
     CDS             complement(886..1734)
                     /locus_tag="JN749_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(1756..3768)
                     /locus_tag="JN749_000404"
                     /pseudo
     CDS             complement(1756..3768)
                     /locus_tag="JN749_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
     gene            complement(3798..4211)
                     /gene="hyfJ"
                     /locus_tag="JN749_000405"
     CDS             complement(3798..4211)
                     /gene="hyfJ"
                     /locus_tag="JN749_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HAY7366932.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            complement(4204..4962)
                     /gene="hyfI"
                     /locus_tag="JN749_000406"
     CDS             complement(4204..4962)
                     /gene="hyfI"
                     /locus_tag="JN749_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HAY7366933.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(4959..5504)
                     /gene="hyfH"
                     /locus_tag="JN749_000407"
     CDS             complement(4959..5504)
                     /gene="hyfH"
                     /locus_tag="JN749_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HAY7366934.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            complement(5514..7228)
                     /gene="hyfG"
                     /locus_tag="JN749_000408"
                     /pseudo
     CDS             complement(5514..7228)
                     /gene="hyfG"
                     /locus_tag="JN749_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            complement(7218..8798)
                     /gene="hyfF"
                     /locus_tag="JN749_000409"
     CDS             complement(7218..8798)
                     /gene="hyfF"
                     /locus_tag="JN749_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HAY7366935.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFFFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(8803..9453)
                     /gene="hyfE"
                     /locus_tag="JN749_000410"
     CDS             complement(8803..9453)
                     /gene="hyfE"
                     /locus_tag="JN749_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HAY7366936.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(9465..10904)
                     /locus_tag="JN749_000411"
     CDS             complement(9465..10904)
                     /locus_tag="JN749_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
                     /protein_id="HAY7366937.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            complement(10932..11873)
                     /gene="hyfC"
                     /locus_tag="JN749_000412"
     CDS             complement(10932..11873)
                     /gene="hyfC"
                     /locus_tag="JN749_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HAY7366938.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGLASALLRLHGSSG"
     gene            complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN749_000413"
                     /pseudo
     CDS             complement(11884..13901)
                     /gene="hyfB"
                     /locus_tag="JN749_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN749_000414"
     CDS             complement(13901..14518)
                     /gene="hyfA"
                     /locus_tag="JN749_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HAY7366939.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN749_000415"
     CDS             complement(14771..15241)
                     /gene="bcp"
                     /locus_tag="JN749_000415"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HAY7366940.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15241..15813)
                     /locus_tag="JN749_000416"
     CDS             complement(15241..15813)
                     /locus_tag="JN749_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HAY7366941.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15959..16837
                     /gene="dapA"
                     /locus_tag="JN749_000417"
     CDS             15959..16837
                     /gene="dapA"
                     /locus_tag="JN749_000417"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAY7366942.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16854..17888
                     /gene="bamC"
                     /locus_tag="JN749_000418"
     CDS             16854..17888
                     /gene="bamC"
                     /locus_tag="JN749_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HAY7366943.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18101..18814
                     /gene="purC"
                     /locus_tag="JN749_000419"
     CDS             18101..18814
                     /gene="purC"
                     /locus_tag="JN749_000419"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAY7366944.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            18982..19845
                     /locus_tag="JN749_000420"
     CDS             18982..19845
                     /locus_tag="JN749_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HAY7366945.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19860..21875
                     /locus_tag="JN749_000421"
     CDS             19860..21875
                     /locus_tag="JN749_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HAY7366946.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAL
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGCLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            21949..22647
                     /gene="ypfH"
                     /locus_tag="JN749_000422"
     CDS             21949..22647
                     /gene="ypfH"
                     /locus_tag="JN749_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7366947.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(22757..22957)
                     /locus_tag="JN749_000423"
     CDS             complement(22757..22957)
                     /locus_tag="JN749_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HAY7366948.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22985..24112)
                     /gene="dapE"
                     /locus_tag="JN749_000424"
     CDS             complement(22985..24112)
                     /gene="dapE"
                     /locus_tag="JN749_000424"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HAY7366949.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIIEQLVA"
     gene            complement(24116..24472)
                     /locus_tag="JN749_000425"
     CDS             complement(24116..24472)
                     /locus_tag="JN749_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HAY7366950.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            24853..24912
                     /gene="ypfM"
                     /locus_tag="JN749_000426"
     CDS             24853..24912
                     /gene="ypfM"
                     /locus_tag="JN749_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HAY7366951.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(25011..28124)
                     /gene="acrD"
                     /locus_tag="JN749_000427"
     CDS             complement(25011..28124)
                     /gene="acrD"
                     /locus_tag="JN749_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HAY7366952.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNAAINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKAEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(28326..30049)
                     /gene="narQ"
                     /locus_tag="JN749_000428"
                     /pseudo
     CDS             complement(28326..30049)
                     /gene="narQ"
                     /locus_tag="JN749_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            30255..32234
                     /gene="aegA"
                     /locus_tag="JN749_000429"
     CDS             30255..32234
                     /gene="aegA"
                     /locus_tag="JN749_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HAY7366953.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDTPGVYDALPFIIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            32302..32877
                     /gene="nudK"
                     /locus_tag="JN749_000430"
     CDS             32302..32877
                     /gene="nudK"
                     /locus_tag="JN749_000430"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HAY7366954.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            33003..34034
                     /locus_tag="JN749_000431"
     CDS             33003..34034
                     /locus_tag="JN749_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HAY7366955.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQSFRLVRYAAEPTCDNW
                     HGPDARPTL"
     gene            complement(34148..36151)
                     /gene="tkt"
                     /locus_tag="JN749_000432"
     CDS             complement(34148..36151)
                     /gene="tkt"
                     /locus_tag="JN749_000432"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7366956.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(36171..37121)
                     /gene="tal"
                     /locus_tag="JN749_000433"
     CDS             complement(36171..37121)
                     /gene="tal"
                     /locus_tag="JN749_000433"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7366957.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            37410..39689
                     /gene="maeB"
                     /locus_tag="JN749_000434"
     CDS             37410..39689
                     /gene="maeB"
                     /locus_tag="JN749_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HAY7366958.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            39982..40317
                     /gene="eutS"
                     /locus_tag="JN749_000435"
     CDS             39982..40317
                     /gene="eutS"
                     /locus_tag="JN749_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HAY7366959.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            40330..40809
                     /gene="eutP"
                     /locus_tag="JN749_000436"
     CDS             40330..40809
                     /gene="eutP"
                     /locus_tag="JN749_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HAY7366960.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            40784..42157
                     /gene="eutA"
                     /locus_tag="JN749_000437"
     CDS             40784..42157
                     /gene="eutA"
                     /locus_tag="JN749_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HAY7366961.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            42681..43372
                     /locus_tag="JN749_000438"
     CDS             join(42681..42927,42927..43372)
                     /locus_tag="JN749_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7366962.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALPPFNIGM
                     LTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHEK
                     VIGAFIEKHMFY"
     gene            complement(43491..44312)
                     /gene="murI"
                     /locus_tag="JN749_000439"
     CDS             complement(43491..44312)
                     /gene="murI"
                     /locus_tag="JN749_000439"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7366963.1"
                     /translation="MRLNMFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNM
                     NAPYGNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPI
                     LSIIEPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLI
                     EEGNLNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAH
                     DQAISIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(44334..45794)
                     /locus_tag="JN749_000440"
     CDS             complement(44334..45794)
                     /locus_tag="JN749_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7366964.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            complement(45787..46065)
                     /locus_tag="JN749_000441"
     CDS             complement(45787..46065)
                     /locus_tag="JN749_000441"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366965.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNIREYKNV"
     gene            complement(46046..46384)
                     /locus_tag="JN749_000442"
     CDS             complement(46046..46384)
                     /locus_tag="JN749_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7366966.1"
                     /translation="MLDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMA
                     EPEGRCVRMLSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEP
                     DLRSCANMLA"
     gene            complement(46843..47049)
                     /locus_tag="JN749_000443"
     CDS             complement(46843..47049)
                     /locus_tag="JN749_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366967.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            complement(47514..48056)
                     /locus_tag="JN749_000444"
     CDS             complement(47514..48056)
                     /locus_tag="JN749_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366968.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            complement(48121..48342)
                     /locus_tag="JN749_000445"
     CDS             complement(48121..48342)
                     /locus_tag="JN749_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366969.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLIC"
     gene            complement(48360..48755)
                     /locus_tag="JN749_000446"
     CDS             complement(48360..48755)
                     /locus_tag="JN749_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7366970.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            complement(49515..50267)
                     /locus_tag="JN749_000447"
     CDS             complement(49515..50267)
                     /locus_tag="JN749_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7366971.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKQK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            complement(51318..52265)
                     /locus_tag="JN749_000448"
     CDS             complement(51318..52265)
                     /locus_tag="JN749_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAY7366972.1"
                     /translation="MYQSATAYVAKPGETLVKHKIATLQALQTLQALPEQVQAAFRDG
                     MNIIRFDGYRWEYKPATGFVRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQEQEQ
                     EQEQEQEQEQNKAAAIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNE
                     EAEEDLRFDSKEEQLPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDEL
                     KLYTWMVSKCRYRGNDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQAL
                     RGKCGKTYRLRIPSKGTAEEASMTAQQHEREAKVSYLVH"
     gene            complement(52560..53765)
                     /locus_tag="JN749_000449"
     CDS             complement(52560..53765)
                     /locus_tag="JN749_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7366973.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWINLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            53954..55315
                     /locus_tag="JN749_000450"
     CDS             53954..55315
                     /locus_tag="JN749_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HAY7366974.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            55336..56223
                     /locus_tag="JN749_000451"
     CDS             55336..56223
                     /locus_tag="JN749_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HAY7366975.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            56233..56892
                     /gene="eutL"
                     /locus_tag="JN749_000452"
     CDS             56233..56892
                     /gene="eutL"
                     /locus_tag="JN749_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HAY7366976.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            56905..>57156
                     /locus_tag="JN749_000453"
     CDS             56905..>57156
                     /locus_tag="JN749_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HAY7366977.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQ"
BASE COUNT        14096 a        14543 c        14784 g        13733 t
ORIGIN      
        1 ttgtattggt ggtttttgtc ggtatattgc tactgatggc gttcgtggta ctgcctatcg
       61 cctggcaaca gggcatctac ttaatccgcg atatgccggg gatgctcaat aagctttctg
      121 actttgccgc cacgttgccg cgccgctatc cggtgttaat ggacgcgggc attattgatg
      181 caatggccga aaatatgcgc agtcggatgc tgaccatggg cgattcggtg gtgaaaattt
      241 ccctcgcctc gctggtcggt ttgctgacca tagccgtcta tctggtgctg gtgccattga
      301 tggtcttctt cctgctgaaa gacaaagagc agatgctgaa cgccgttcgc cgggtgctgc
      361 cgcgtaaccg tgggctggca ggacaggtgt ggaaggagat gaatcaacaa atcaccaact
      421 atatccgcgg caaagtgctg gagatgatcg tggtggggat cgccacctgg ctggggttct
      481 tgctcttcgg gctgaactat tcgctgctgc tggcagtgct ggtcggcttc tcggttctta
      541 ttccgtacat tggcgcattt gtggtgacca ttccggtggt tggcgtggcg ctattccagt
      601 ttggcgcagg tactgaattc tggagctgct tcgcggtgta tctgattatt caggcgctgg
      661 acggcaacct gctagtaccg gtgttgttct ccgaagcggt taacctgcat ccgctggtga
      721 ttattttatc ggtggtgatc ttcggtggtt tgtggggatt ctggggcgta ttcttcgcca
      781 ttccattggc gacgctgatc aaagccgtga tccacgcctg gcccgatggg caaatcgcgc
      841 aagaataatc ttcagcccga cgaactgttt cgccgggctg atttttcagg attcctgacg
      901 taaataaata gcccgataac aaatactcac cagcactgca ccgccgataa tattacccag
      961 catcaccggg agcagattgg cggtaataaa atggctgacc gtcagtgccg gaaaattgtc
     1021 tgcgctactg tgcgccagtt gccagaagga ggtaggggcg aaatggcgaa tggcaatggc
     1081 gaacggaatc acaaacaaat tggcgataca gtgctcaaag ccactggcga caaacagggt
     1141 gatggacaaa atcatggcga cgattttgtc gcataacgaa cgtccgcaat aactcatcca
     1201 cagcgccagg caaaccatca gattgcacat aatgccgagg ctgacagatt cagtaaatgt
     1261 atgatgcatt ttgccctcgg cgcagtgtaa aatcgcgact ccccacatcg cgttttcact
     1321 catcaccagc ccggaaaacc agattaacaa actgaaacag gcaatacctg ccagattacc
     1381 gcaggccacc agaagtgcgt taatcagcca agttcgccaa ctaataacgc cccgactttt
     1441 tgccattacc gtcattaccg acgaggtgaa caggctggtg ccgcaaaccg ccagcaaaat
     1501 aaagccaagt gtaaagcaaa ggccgcccac cagatgggtt aatgcctatg aagacggggc
     1561 atcggcgata acggaaaggt aaaaagtaaa tccgatcgcc ataaacacgc cagcggacat
     1621 cgccattaaa aagaccgaca ctttactgcg ggcaatttta tgggcggcaa tccgttcagc
     1681 gattgccgct tttctggcgc tcaactgcaa gtcgaaagag agtttgtttc tcatgcatac
     1741 tcctgcaaaa gcagattaca acacctcgcg aaccgagatt cccagacgct gcattcgtga
     1801 cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg ccacggggcc cggcaacaat
     1861 gccattggtt tctcgcaata cctgaacaat gcgctgacgt tcttcttcgt cattctctgg
     1921 cgtcgtcggg tgcagcaact gcgccattga actgcgaagc gctgaatctt catttagcgt
     1981 cggcagtaaa cggctttgtc ggacattaag atgtaaattc agactgttac cgcgagtcag
     2041 cagtaccgct cgctcaatca cgttttccag ctcgcgcacg ttgcccggcc agtcccacga
     2101 catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt gaacgattca tatggcgcgc
     2161 cattttttgc gtgaaatgct ttgctaaaag agggatatct tccggacggt cgcgcagcgg
     2221 aggcaattcc agtgggaaga cattcaggcg ataaaagaga tcgctgcgaa actggcgatc
     2281 ttcaaccatt tgccacaaat cacggttggt ggcggcaatg actctgacat ttaccgggat
     2341 cgttctactc ccgccgagac gctcaatctc ccgttcctgc aatacgcgca gcagtttagg
     2401 ctgaagttct aacggcagat cgccaatttc atcgagaaac aacgtgccgc catcggcaat
     2461 ttcaaaacga ccacgatggg tattaatcgc accagtaaac gcccctttgt catgaccgaa
     2521 taactcactt tccagaagac tggcggggat ggcagcgcag ttgattttga ccagcggctt
     2581 gtcgtgtcgc gggctaagtt gatggatcgc tctggcgatc acctctttgc cggttccggt
     2641 ttcaccgcaa atcaacaccg tactgtcgct cttcgccaca atatctacct gctggagcag
     2701 gtcttccatt gcctggcttt gatagataat gtcaccgacg cccagattcg aaaggagctg
     2761 ctcgctaagc tggtggtttt cttgctgcaa actttcctgc aaatcggtca ttctacggca
     2821 ggcatcggca ttgccaacgg caatagcgat gcgatcggct atgtgttgta gtagctggca
     2881 gttttcctca ctaaagagag tggaagaggt atgcgccagc aacaatgcac ccggtgtatg
     2941 gttgccaaag gtaagcggta taaggagcgc agattcgcag ccgttagatg caagtaagag
     3001 taataacggg tcgcgttgcc agagaaacag atcatctgca cggtgcgtca aggtcggttg
     3061 tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca ggcatattgt gtggcagaca
     3121 cgcacaatgt gaggcagaaa gatcgctgca ccacaggctg aacttctcgt tctttcgatg
     3181 atcgcccagt accatactga ctgaagccag accgaaaaaa tgatggatct cacgagcgac
     3241 gtcagcgatc agatcgtcga gatcaagatg tgacagcact gcattagtga tatccaccag
     3301 tacccgatga tgatcgcgat ctttctccag caaatgcagc gccacctgct gttcttcatc
     3361 attgagccat gattgcagaa ccggggtgat aatgcgtgcc agccagttaa acagtgtgca
     3421 atcctgggga gtaaaacggc tgccgtgggt acgaataaag cgtagctccg ccattcaact
     3481 actgccgaga aggacggcgt gacgcgaata atcgaccatc tcgccctgat actctgttgt
     3541 tgccgtcaca tcgctgccat catcatggta gcggtaatag aagttgcttt tgagaacatg
     3601 cagttcaata agttgtaccg ggctgaacgg cggcggcagc gggatgaagg cgcgtaataa
     3661 agacgccttg ccgtgtttct gcgctaaccg ctccgcgagc attccgttta ccgcttcaat
     3721 tgttattcct tgcggcgggg caaacatcgc ttcgtctgac atagccatca acagtcctta
     3781 ttaatgagaa atccttctca cgcaattttt ctggccatca gatagatagc cggttccagc
     3841 acaatttcat ccaacagatg aatgaattga atgctacgcg cttgccacac agacgtcgcg
     3901 tcatctgcca gtggcgcgaa tgcccgggtc aacagggcgg tgtggctggc gtcaataaca
     3961 atctcaccaa aagtcatcac ccccagcatc ttacgtcggg cttgctcctc ttcgaccagt
     4021 gcaagccaat cttcgtattc cgtcagtggg cagcggaagg cgacattcag acaatcaatc
     4081 acgccaacgt gatgaccgat agccagcgag taatacatta cctgagagct gtgttccggc
     4141 atctcgtcgc tactggcgac aaactttttt cgcagcgtcc agaaaacaat ttccccgcac
     4201 tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc tgaacgatac tattgagacg
     4261 tggatcgtcg gcatcgcgca accaggtgtt aacccgattt cctgtaggat cgtcgcttaa
     4321 atggcgcagg agccgatcgc aaatttctcg cccctgacga tagcccgcca gccgccgcgc
     4381 ttctcgctca atggcgatac gctgtgatgg cgggatctgc ggccacaacg gttgcatagt
     4441 caccccggtg ggatcgcgat aatccaccgc gtgaatcttc tgttgcagca aaccgagcgc
     4501 cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg cagccgggga tccaaacatc
     4561 aatggggaca atggtgtcgc tgccgcccca gacgctgtag agatcgtgga aaataccgcc
     4621 gccgacaccg cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc
     4681 ccgaagtgca ggcatacgca tcgcccgggt gactgcgcca gtaaataaca aaatatcggc
     4741 gtgacgcggt gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata cgggggtgat
     4801 ggcagcaaaa atttcgattt cacaggcgtt gcagccgccg caatcgatgc gataaacgta
     4861 agccgagcga cggatatcct gtagcaaatg ccgcttcatc ttttgcgttt gctcgtccag
     4921 cgtgatgggc tggctgacat attgcgtaag cactggactc atagctgctc cttagccacc
     4981 agcggtacat ctgtatcgtc gttgatcagc gtcgcgcgtt gtttgcactc cgggcagacg
     5041 ctcgcttgcg cccataacat ttcgcggttt tgtggagcat tttgttgctg tgccaacaat
     5101 tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc gttcgcaacg gctgcaacgt
     5161 tgtagatgga acgtcgcgtg ggtatagaga tcggctttat gggtgacggt cagttcaaag
     5221 ttattggtaa gctggatggc tctggtcggg cacacttctt cacaacgtcc gcagtaaata
     5281 caacgcccca gatagagctg ccaggtgcgc gaattttgct ggtcgtcggt ctggatagtc
     5341 agcgcatttg ccggacaagc acaggcgcag gcaccgcagg caatacattg gctgggcatc
     5401 aggtccggtt tcccgcgaaa gcctgggctg acctccagtg gcgcgaaggg atatttcacc
     5461 gtcggggttc cggcgcgcat aatagttttc agtaacttca gcatcttctg tccttacttc
     5521 agcggcgaac ggttacgatc aatgccgtag cgttcgatct ctttatacag cacggttttt
     5581 gactggcgct tgcgcacatc taccagcgtc acacggtcgg tacaggagta gcagggatca
     5641 aggctaccga taatcagcgg tgcgtcagaa acggtattgc cgcgcagcat gtaacgcaac
     5701 accggccagt tggcgtaggt ggcggcacga cagcgccagc ggaacaattt ttggttatca
     5761 ccgagcatgc tccagtgcac gtcttcaccg cgtggcgctt caacaaagcc cagcgcgaat
     5821 gcgtgaggtt tatagctaaa gccttcggtc agcagtgggg tatccggcat gttgtcgagg
     5881 gcaaattcca gcattgccag cgaatcaaac gtctctttga cacggaccat cacgcgggag
     5941 aaaacatcgc cgccggtaaa ggtaaacagt gtttttggaa tattgccgta gtcggcgtag
     6001 gggtgatcaa agcgcaaatc acgggcaaaa ccactgcgcg gatcagcggc cctacagggc
     6061 tataatcacg ggcgatttgt cggtcgagaa tgccaatgcc ctgagtgcgt tgttccatat
     6121 tcggcgtagc aagcagcatc tctaccagct ccgacacgtc ggagcgcatc tcgcgcacca
     6181 gtttcagcgt ttgcagacgt tgctctttga gaatatcgcg gcgaacacca ccaatcagat
     6241 tcagaccgta ggttttacgc gacccggtca gcaattccgc catcgtcatc gacttttccc
     6301 gcacgcggaa aaattgcata aagccggtat caaaaccaac aaaatggcag gagaggccaa
     6361 ggttgagcaa atgactgtgt agccgctcga cttccagcag aatcgagcga atagtatgtg
     6421 ctcgttgcgg cacctcaatc cccagtgcat tttcaaccga gttggtatag gccacactgt
     6481 gggcaaaacc gcaaatccca cacacgcggt ccgataagaa ggtcacttcg ttgtagccca
     6541 tccgcgtttc tgccagtttc tccatgccgc gatggacata aaacaggcgg taatcagcat
     6601 cgacaatttg ctcgccatcc acaaacaagc ggaagtgacc cggttcatag gaggtgatat
     6661 gcagcgggcc gacagggatc acccgcgcat cgctgttgcc ctcattgata aacgcatacg
     6721 tttcggaatc tgtcgtcggt tcagggcgca gtcgataatc catcgcatct ttgcgcagcg
     6781 gatgcatatc ttccggccag tcatcgggca acaccaggcg acgctgatcc ggcaggccaa
     6841 ccggaatcag cccgtacata tcgcgaattt ctcgctcgcc ccagaccgcg gcagggacgc
     6901 gcggtgtgac tgacggaaac tcccgactgt cggcatcgac cagtgccttc accacgatcc
     6961 agcatttttc ggccccttcc attgaaaggg cataataaac cgcgtaatga ccgttaagtg
     7021 tccgctcgtc gttgccaaac aggactggaa gccagccatc atgttgataa taaagataat
     7081 gtacaacgtc aggcagcaga ttgattttca ccgtaatggt gacctgttca ggcgtttgtc
     7141 gctcttcatc cagcaccgcg ccggggaact gcgttttcag ggcggcgaga atcgcttcgc
     7201 cacgatttga cgatgaatta acgttcactg tgtttctccg aaacagatgc ggtgcctgaa
     7261 ggcaaaaaat catgccaggt gctacgttgt gcaggcaggt cgtgcgtccc tgagaggact
     7321 atagtggaag cgcccgccag gatcctgatg acaggttgtg gaatatgcgt tcccatcgcc
     7381 agcatcatga ctagcagaat caccattggc gaggtggtca accagccgag atcaccccgg
     7441 ttaacggcct gcggcggttt cgccattaac acccgcgcag ccatccgtac caggcccgcg
     7501 agcaccagcg ttaacagcaa taacagcagg acgataatca gcaggtgatt acgtgccagt
     7561 cctgcggtaa cggtcataaa ttcgctaaga aaaatgttga agggcggcat ccctgccagc
     7621 gccagcgcac cgccgccaaa cagcacggcg gtaaatggca tgattttgag catcccacag
     7681 acgacgttga gatcgcgcgt gccgtacttg agcagtacat tgccggaacc gcagaacagc
     7741 agcgtttttg ccagactgtg gtttaaggtg tgcagcagcg cggcaaaaat tcccagcggc
     7801 ccgccaatgc ctagcgccac cgcgaccagc cccatgttct ccacgctgga gtacgccagc
     7861 agacgcttaa tgtcccgctg taccagaatg aaaaatgccg ccacggcaac cgacaacatg
     7921 ccgaagatga gcaacaaccg gttggggaaa tcgctgccga tggcctggca aataatgatg
     7981 taatagcgaa tcagcaccaa cagcgcgcag ttcagcaata cggcggagag cagggcgctg
     8041 accggactcg gcgcttcact gtgagcatcc ggcagccagg cgtgcatggg aaatagcccg
     8101 gttttggtac caaagccaat tagcacaaac acaaaggcca acagcattaa tgttgggtca
     8161 agcaaggacg attgcttaag aacctcgctc cagaatatcg ccatttctgc ctgcggcata
     8221 acgctggcgg cgttggcgta taccagcacg gtaccgaaca gaccaaaagc gacaccaaca
     8281 gtacaaataa tgatgtactt ccatgctgat tccagcgatg aacgctgacc gtaaatgcct
     8341 accagaaacg ccgagcttaa ggtggtggct tcgatcgccg cccacatcac aatcaggtta
     8401 ttgctggtaa caaccagcag catggtgaaa aaaaacagat ggaagaaacc gtagtaatcg
     8461 cacagtgtaa cgggtgaaag ctcgccgtgt gccacttcat gacgcatgta gccaatcgag
     8521 taaacaccgg tgagaaagcc aatcacacca agaatggcga ggaacaaacc gcccagacca
     8581 tcaatatgaa gccacagtcc cgcagcgaat atttctcctg catcagcggc agtttggacc
     8641 acccagagtg ccagaatcag cagcagtgtg atccctaagc tatgtaatac agtcaccgta
     8701 caagtcgcag agagcccccg tttccggcag gcaaaacaga gcagcgaaaa aagcagcggc
     8761 gtgagcagga gtaaagcgaa catcacagaa taactcatct cattatccct tcagcgcggt
     8821 caagttgttc acgtccagcg tgccgtgggt acgccatatt tttcttgcca gtaacaccat
     8881 cacaatgacg gcgaagatgg cgtcggtagc gatacctatt tccaccagtt ccggtgctcg
     8941 ccaggcaaga agcgccagca ccagatggga gccgttttcc atcaggcagt aaccaaaaat
     9001 ttgccgcagg atattgcgct ggctgacaat gcacagcagg ccaagcagaa aatgacctaa
     9061 cgctaccgcc agcgccggtt tcagcccggt agccatcggt agcttcacgg gctgaacgac
     9121 aaatgcgcaa agcaggacaa ttaacgccgc gagcagtgcc atcattgccg gaccgaataa
     9181 cgctttttcc gggatgttct ggggaatatt tcgtgcagcg taagtcatga ttaacggtac
     9241 cagcagcact ttggtgataa aggcgctggc ggaccagatc agcagttgct ctgcggcgaa
     9301 caggcacgag agagtggcga aaatagacac cagcaccagc gactggcagg cgtaaaatcc
     9361 gcatgacagg cgatagcttt tgacgctaat cacaaacagc gatgtcagca tcatcagtcc
     9421 cgccagatta tttacgatca tagaaccggt cattcatcat ctccttactg caaccaggtc
     9481 gcggcgatta cgctggaaat cagcgacatc acaatcagta caatcaacac caggcgcatt
     9541 gaccctggca gcggtgcggc atcggcgacg gcctcgctcg gtttgcccgg cacgacgcga
     9601 ccaaaccagc gaataaacca ggcgaaactg gcgaccgatt caatcatcag cagaatcatg
     9661 gcgggcagca ggatccagta ctccactgac aacgcaaaac cggcggcaaa cagcgggaat
     9721 ttactgaaga agccgttgaa cggcggcaca ccggtaattg ccagcgctgc cacgcagaaa
     9781 cccacgcctg gcaacggcag ggtgtgcaat acgccacgca gacgcggcaa caagcgcgtg
     9841 ccgcagctgt aactcagcgc acctgctaca aggaaaaaca ggcttttagc gaacgcgtgg
     9901 ttgacgatgt aggcgatgct accctccagc gccagccgcg agccgaagat ggagagcgac
     9961 aagccgaaga acatccagcc aagttgagtg atggtcgacc aggccagcaa ccgcttcata
    10021 tcctgctgtg gcaaatacat cagaaagcca taaagaatgg tgaccagtgc catgaccatg
    10081 ccaacgccgc caatcacatg cgggatattg ccgccgtcga taatggcgcg ggcaaaaatg
    10141 taaacgccca ctttcaccat cgatgcggcg tggagatagt cgctgatcgg tgttggcgct
    10201 tccattgcgt ccggtagcca cgcttgcatc ggtagctggg ccgatttccc ccacgcggca
    10261 aacaggatgc cgccataaac cagataacgt gcgtcgccat gtaactcgct catcgcgcta
    10321 agcacaaacg ttccggtctg caaaaacagc gtggcggcgg caagatacaa ccccaacgaa
    10381 ccgatatggg tgataagcaa cgcttttagt gctgaacgct gcgctttatc gctctggtaa
    10441 taactgatca acgcccagga gcagccgccc gtaatttcaa aaaacaacaa ctgaccgagc
    10501 agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaccagcaa aaatgcgtaa
    10561 taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata acccgtggag
    10621 tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt actcacgcga
    10681 tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaacgtcaa cgtgacggca
    10741 actttatcgg cctgataaaa cgcggagatc agcgacaaca tgcacagcgt ggtcagcgcg
    10801 gcgaacaaaa ccccccattc ggcggcccga cgttgtggcg aaaacgaaac gaccagtgcg
    10861 ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg tcagtgctcc
    10921 ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc caagccaagc
    10981 caggtcacat ggtgaatgag taaaaaacgc ccgcgtgcca gcgtgttttc tgcgattgag
    11041 gccagtacaa aaatcagcaa aaccttgagc agcgtaacga caagtgaagt cagcaggcag
    11101 gcgagagaaa gttcttgcgc gcgcccaaag ggcagaaaca gggccacaaa cagtgctgcc
    11161 atcacgacct gtttcagccc ctgcccccat ttcgctagcg ccagcccggc accggaatat
    11221 tcggtcagcg ggccttcctg taattcctgt tctgcttcag caacatcaaa gggaattttt
    11281 cccatctcaa tgaagcaggc aaaaccacag gccagtaacg ccagtacggt ggttagcggc
    11341 gagttccagc ccatcgccag cgtattgctg atcatctcga tatgcgtgga acctgctatc
    11401 agcgccaata ccagcagtga gagaataagc attggttcga ccagaatgcc gagcgtcaac
    11461 tcgcgactgg caccgactcc cgcaaacgga cttccggtat ccagcccgga aagagcaaag
    11521 aaaaaacgaa acagggcaag aagatagata agggtgatca gatcgccgcc gcccgcaaaa
    11581 ggggaaacgg taataaatag tggtaaggcc atcgccagca ccagcatgct gctgattaat
    11641 acccacggca tcaggcggaa catcagacct gaagatgtcg gcgcaacttc ctggcgttta
    11701 aacagtttgt ggatatcgcg ataatcctgc cagatccccg gcccgcggcg ggagtgcata
    11761 cgcgcgcgta tctgccggga aatacccgta aaaagtgggg ttagcatcag cagaatcacg
    11821 gcctgtgcta acgcaaaaat gaccagatat ccgtcgcaaa gagtttgtct catggtgatt
    11881 tccttagacg gcaatagcga ttagcagcac aaccagtgcg gcgaccacat acaggcaata
    11941 gagacgaaag tcgccgcttt gcagatgctg tatttctttg accagccgtt gggtggcgct
    12001 cacgatgggg cggatcaccc gctcatccca gaagggttct gtgctctgag ccctggcggt
    12061 gacgtgcgca agacctttat ttagcctcag cgtagggtcg agctgttgtc gtagacgaaa
    12121 taacgcagag aagaccacac gcagcggctg catcacgcca ttgcctgacg gagccatcgc
    12181 gttttcccag ccgtagccgc atgcccaggc atctcctgtg cgacgaaacg ctgcgcggcg
    12241 cgaacgacac cacagccagt aaaggcctgg cagcaaaggc atcgccagta gtaacagcaa
    12301 cagtaatgaa ggagtgaccc gtgtatgaaa cgtgccgggt acaagtgcta ttccgctggc
    12361 gacagtgacg ggaggggtat tggtaaacgc atgggcaata tgcattatct tcggtgccag
    12421 ccaactggca ctaagcgcaa ttaatacgca gagtgccgcg agcagtagca tggcgacgat
    12481 catcgtattt ggcacttcct gtgcctcttc agcgtgtgta ctgcgcggcg caccacagaa
    12541 agtaataccg tacattttga cgaagcacat tactgccagc ccaccagtga ctgccagcat
    12601 taccatagca ataggacccg caagttgtag cgctacggct tccacacgac ttagtgagaa
    12661 cagcgactgc caggtgtacc attcgctgat aaaaccattc agcggaggaa tggctgatat
    12721 cgcgaggcaa ccaatcaggc atgctgcggc tgtccacggc atccgtttcg ccagtgcccc
    12781 cattttttcc atgtcgtggg tatgcaaacg cgaaataatc gcgtccgcgc cgagaaatag
    12841 cagccctttg aacagcgcat ggttgagcag atgaaacagt gcgccgagca gtccaaccac
    12901 ggtgagcagc gggtcgtgca gtgacagacc gtccatcgcc acaccgactg ccagcaaaat
    12961 aatgccgacg ttttcgacgg tactccaggc cagcagccgt ttgatatcct gttccgccag
    13021 cgcatatagc acgcccagga gcgcggagat tgcgccgatc gccatcacca gaatgcccca
    13081 ccacagaggc aaacccgttt gcgccagcag atccatcgct actttcagga tgccgaaaat
    13141 accgattttg accattacgc cagacatcaa cgccgaagcg tgcgatggtg cggcagggtg
    13201 agcgcgcggc aaccagctgt gcaacggcat catcccggct ttcgcgccaa aaccgaaaaa
    13261 ggccagcaga aacaccgccg acgccagccc cggagaaagt gaaagcgtgc gaaactggca
    13321 aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcataat cagcaccgat
    13381 ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt atcctgcctg
    13441 gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat gaaccaaaaa
    13501 gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaagattcat aaagaagccc
    13561 atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata gagcgaacaa
    13621 accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc cattcgcagg
    13681 gttaaatcgg caaagtcaaa aggggtggca aaatgggcga ctaaagttac accagcatgt
    13741 aattgcgtaa ttccgctgat gatgccaatc acgccgccca ccagcgatgt gatgccggaa
    13801 agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact ggcaaacaga
    13861 tagaggatca gcgaccaggt taataattgc agggcatcca ttagtgttgc tcctgagtga
    13921 gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc tgtttttctt
    13981 tcatctgacg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc gggcaagcgc
    14041 gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc gcgacggtct
    14101 ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct tcgccgtctt
    14161 tgagtgagcc ttcagcctga aaaacatatt gcgcatgggc attcaccgga cggcttcctg
    14221 aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca atacagaggc
    14281 tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg cagacctgca
    14341 ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgattgtt gacgtcttcg
    14401 ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc gaacaggcag
    14461 cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag cggttcatgt
    14521 cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaaggg tgccattctg
    14581 attaactcaa tctattcaat aagttatgta ttaactggat tctgaacggt acaaaaatga
    14641 tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg aaagcgagaa
    14701 tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag cacggaatgg
    14761 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
    14821 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
    14881 gatgaatacc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
    14941 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
    15001 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
    15061 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
    15121 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
    15181 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
    15241 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
    15301 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
    15361 tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
    15421 ttgtgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
    15481 gcgttcaatt aaatgcgggg agtctgcaac atcgacctga acccagacag atgctggcat
    15541 tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gttcggcacc
    15601 tttcaacggt aacgttgatt caatcagagt gatggcattc catgaaccgg aaagcagcat
    15661 gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
    15721 actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac ccaacgcagt
    15781 gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa ggggtgagtt
    15841 gttcttaagg aaagcataaa aaaatcatgc atacaacaat cagaacggtt ctgtctgctt
    15901 gcttttaatg ccataccaaa cgtaccattg aggcacttgt ttgcacagag gatggcccat
    15961 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
    16021 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
    16081 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
    16141 gatgacgctg gatctggctg atgggcgcat tccggtaatt gccgggaccg gcgctaacgc
    16201 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg tcggctgcct
    16261 gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
    16321 catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
    16381 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
    16441 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
    16501 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattgg gcggtcatgg
    16561 ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc
    16621 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    16681 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    16741 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    16801 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    16861 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    16921 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    16981 aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg gtgacctccg
    17041 gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg ctggacattc
    17101 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    17161 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    17221 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    17281 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    17341 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    17401 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    17461 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaatc
    17521 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    17581 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcgtcgctg gaaaaagtgg
    17641 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    17701 tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca tccggtgact
    17761 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    17821 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    17881 gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga tacgcaaacg
    17941 tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    18001 gtagttcgga taaggcgttc acgccgcatc cgaaaaaaca tccggcacac cagacagcaa
    18061 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    18121 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    18181 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    18241 tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    18301 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    18361 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    18421 cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa acgatgccat
    18481 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    18541 cctggcacgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    18601 tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggtgaagt
    18661 ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacactgga
    18721 gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    18781 cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat cttgccgtgc
    18841 agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca ttcctccgcc
    18901 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    18961 gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa
    19021 gacaggcgca acagctctgg cggtccgtca atgggcggtc ccggtttccg tctgccgagc
    19081 ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta tggcgttgat
    19141 ttaaccgggt tgatgaccgg gcagccggtt tctcaacaac aatcaacgcg gtcaattagc
    19201 cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    19261 tggggacaac agttcgaaaa gatgggtaag acctatcagc aaccgaagct ggtcatgtac
    19321 cgtggaatga cgcgcaccgg ctgcggggcg ggccagtcca ttatggggcc gttctattgc
    19381 ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt
    19441 ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg
    19501 cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    19561 gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    19621 gggcatagta tgcagcagca aggcgttctg gaaactggcg atctggaaga ggcgctgaat
    19681 gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca
    19741 gacagtttca cacatggcac ttctcagcaa cgctgcagct ggtttaaacg tggtttcgac
    19801 agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaatttcc ggggcaggga
    19861 tggctgaact gactgcgctt tacacattaa cagcgcaaat gaaacgtgaa gggatctgcc
    19921 gcttgctggt gttgagcgga gaagagggtt ggtgttttga tcatgctctt aagttgcgtg
    19981 atgccttacc tggcgactgg ctgtggattt cgccgcagct agatgctgaa aaccactgtt
    20041 caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc
    20101 gccacggctt tgatgccgct gcctttgcag cacttagcgg aacgttgaaa gcgggaagct
    20161 ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat gccgactcgc
    20221 tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag catctcaaac
    20281 gcgtacttac ggcggataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg
    20341 cgcattttac tccccgtact gactggcacc ccgcgaccgg cgcaccacaa ccagaacaac
    20401 agcaactctt acagcagcta ctgaccatgc cgccgggcgt ggcagcggta acggctgcgc
    20461 gtgggcgcgg taagtcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga
    20521 ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg ggcgagaagt
    20581 ttcgctttat tgcaccggat gccttgttag ccagcgatga gcaagccgac tggctggtgg
    20641 tcgacgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg cgttttcctc
    20701 gaacgttgtt aaccactacg gtgcagggct acgaaggtac cggacgtggt tttttgctga
    20761 aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg atccgctggg
    20821 cacagggatg cccgctggaa aaaatggtta gtgaggcact ggtttttaac gatgaaaact
    20881 tcacccatac accacaaggc aatatcgtca tttccgcatt tgaacaaacg ttatggcgaa
    20941 gcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac taccggacct
    21001 cgccgctgga tttacgccgg atgatggatg cactagggca acatttttta caggcggctg
    21061 gcgaaaacga gattgccggg gcgttgtggc tggtggatga gggcggatta tctcaagaac
    21121 tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg gcccagtcgc
    21181 tggcggcgca cggcagcaat ccactggcgg cgacattgcg cggaaggcgg gttagccgga
    21241 tagcagttca tccggctcgt cagcgggaag gcacagggcg gcaacttatt gctggagctt
    21301 tgcaatatac gcatgacctc gactatcttt cggtgagttt tggttacact ggggagttat
    21361 ggcgtttctg gcaacgctgc ggttttgtgc tggtgcggat gggtaatcat cgggaagcca
    21421 gcagcggttg ctatacggcg atggcgctgt taccgatgag tgatgcgggt aaacagctgg
    21481 ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcgcag tggaatggcg
    21541 aaatgcttcc tgttgatcca ctaaacgatg ccatcctttc tgacgacgac tggcttgaac
    21601 tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc ttattgcgtc
    21661 tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gtgtttacag aaaaacgcca
    21721 gtgacgcgca gttatgtacc acacttaaac tttcaggccg caagatgtta ctggttcgcc
    21781 agcgggaaga ggccgcgcag gcgctgttcg cactaaatga ggttcgcact gagcgtctgc
    21841 gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct cccatggtca
    21901 ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat gaaacatgac
    21961 cattttgttg ttcaaagccc ggataaacct gcacaacagt tgttactgct tttccacggt
    22021 gtcggggata acccggtggc aatgggggaa ataggcagct ggtttgcacc gctgtttcct
    22081 gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc ggggcgtcag
    22141 tggttttcag tgcagggtat tacggaagat aatcgccagg cgcgtgtgga tgccatcatg
    22201 ccgacgttta ttgaaacggt acgctactgg cagaaacaga gcggagtagg ggctaacgcc
    22261 acggcgctaa ttggtttctc acaaggcgcg attatggcgc tggagagcat taaagccgaa
    22321 ccgggccttg cttcgcgcgt cattgccttt aacggacgct atgcgagcct gccggagacc
    22381 gcgtctacag ccacgacaat tcacttgatt catggtggtg aagatccggt tatcgatctg
    22441 gcgcatgcag ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt gacgctggat
    22501 atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaatttgc cctggatcac
    22561 ttgcgttata ccattccgaa acattatttc gaccaagcgc taagtggcgg taagcctggc
    22621 gatgatgatg tgattgaaat gatgtaaaag acagattacc tggcctgata agacgcgtga
    22681 gcgtcgcatc aggcatcgtt caccattgcc ggatgcggcg ttaacgcctt atccggccta
    22741 cggttgagca ctcagcttac ttcttcggtt gatccttttt cggccagtcg tcgtcatcgt
    22801 cccacttatc gttgaaatca cgatgcgggg gaagctccgg cttgttggcg agaaatttct
    22861 tatggtcgac gcgcttgaga tctttaatca cgttcagcag gacgccgacc aaaaagacaa
    22921 tcaccagaat ccaccaatat ttagccagcc agtccatgct tatttcctct tgcagaacca
    22981 ctcatcacgc gacgagctgt tctatgatac gttgatacat acgggcaagt agctgcaggt
    23041 cggcagcgtt cacacattca ttaattttat gaatagtggc attgaccggc ccgagttcca
    23101 ccacctgcgc ccccatgcga gcaataaagc gcccgtcgga cgttccgcct gtggtcagta
    23161 gctgcggttt aatttcatta tagtgctcaa ccgcgttaac gaccgcatcc accagtttac
    23221 cgcgcgcggt caaaaatggc tgcccggaaa gccaccaatc caccgtatag cgcagttgat
    23281 gtttttcaag cagggcaagt acctgcgctt tgatcatctc atcagtcagt tcggtgctga
    23341 agcggaagtt aaactgcaca aacagttcac ccggaataac attgttactg cccgttcccg
    23401 cctgaatatt ggcaatctgc atactggtcg ccgggaagaa ttcattgccc tgatcccact
    23461 caatagccac taattcatta aggaaaggtg ctgcgcgatg taccggattg tcagccagat
    23521 gtgggtaggc aacatgcccc tgaacgccat gaatggtgag gttgcaggtt aacgatccgc
    23581 gacgaccatt tttcaccaca tcacctacca cttcgatact cgacggttcg ccgaccaggc
    23641 agtaatcgag acgctcatta cgtgccatta acgcctcgac gacttttacc gtaccgttgt
    23701 gggcactggc ttcttcatca gaagtgatca gaaatgccag tcgccccgta tggttgggat
    23761 gttgtgcgac aaaacgttct gctgctacca ccatcgccgc cagcgagcct ttcatatctg
    23821 ccgcaccgcg cccgaataac atgccgtcac gaatggtggg ttcaaacggc ggattgatcc
    23881 aacgatcggc gtcgccaggc ggcaccacgt cggtatgccc ggcaaaggct aacgtttcac
    23941 cttgcccacg ccatgcccaa aaattctgcg tatcggcaaa atccatgcgt tcaacggtaa
    24001 aaccgatcgc ctgcaaacgt tcaatcaaca aagcctgaca tcctgcgtca tcaggactca
    24061 gggaagggcg gcgaataagc tgttgtgtca gctcaataac cgggcacgac atagactaca
    24121 cctcatggaa aaattgctga taactggaat cactgaaacc cagcagcata ggcttaccag
    24181 gcgcgtagag caatggacgt ttgataattg caggcatttc agtcattaat gccgccgcag
    24241 aggccgcatc ggtgatttta ttgcgggtag tttcgtccag tttacgccag gttgtaccac
    24301 gggtgttgag taacgcttcc cagcctaatt cgttgataaa accgttcaat aattcgctgt
    24361 ccagcccatc gacgcggtaa tcatgaaaac gatagtcgat gttattggct tccagccaac
    24421 ggcgagcctt tttaatggtg tcacaatttt tgatgccgta aagtgtaacc ataatgaatc
    24481 ctttttcgat ttatgagaat aaatactcat ttaagggtga aaacattttg cctgcaaatg
    24541 attatcagaa aatataacat tatcgaagta ttcaaattaa caactattaa ataatattaa
    24601 acatttttaa cgtaaacatt aagaatattc atcttgttat ctgtctgtat tttaaccaga
    24661 attaaataag tattagatgc cgtatgaata tctcgaaatg ttgctgtgta tcacatacaa
    24721 attatcaccg caggaccata gtatttacat aaggtttgtc ccccggaggt agtcatcgca
    24781 gcaatcgagt aaattagctt caccacggta tattttttcc gtagaatacc cataattata
    24841 agagaggttg ttatgattga acgtgaactg gggaactgga aagactttat cgaagttatg
    24901 cttcgtaagt aattcctgag acaaagtata aacagcaaga acccgctgag caggttctta
    24961 atcggaaagt tgaaaaaggc gacacattgg catgtcgcct tttttattgc ttattccggg
    25021 cgcggcttca gcgggaagcg gcggcgcacc agcacaaaga acagcggcac gaagtaaata
    25081 gccagaatag tagctgaaat catcccgccc attacgccag tacccaccgc atgctgacca
    25141 ccggaaccgg cacccgtgct ggttgccatt ggcaatacgc cgaagataaa tgccagcgag
    25201 gtcatcagaa tcgggcgtaa acgctgacgg caggcgtgga gcgtcgcttc aaacaggtcg
    25261 tggccttttt ggttcatctc gttagcaaac tcgacgatca ggatggcgtt tttcgccgat
    25321 aaaccaatga ccgttaacag gcccacctgg aagtaaacgt cgttttccag cccgcgcatc
    25381 caggttgcca gcagcgcgcc gattaccccc agcggcacga ccagcattac cgagaacggt
    25441 accgaccagc tttcatacaa tgccgccaga cacaggaata ccaccagcaa ggaaatggcg
    25501 tacagcgccg gagcctgcgt gccggaaagc cgctcctgat acgacatcgc cgtccactcc
    25561 agaccaaagc cgtttggcag ctgcttcact aacgattcca taatatccat cgccgtaccg
    25621 gtactgaccc ccggtgcggc ttccccaaca atctcaaccg cagaataact gttatagcgt
    25681 tccagacgcg gcgagcctgt ttcccagcgt gaggtcgcga aagcagagaa gggcaccatg
    25741 ccgccatctt tatttcggac ataccagaga ttgatgtcat ctggcagcat gcgatacggc
    25801 gcagctgcct gcacatagac tttcttcacg cgaccgcgat ccataaagtc attcacatag
    25861 ctcgaacccc aggcggtttg cagtgtgtcg ttaatatcgt cgatatcaac gcccagcgcc
    25921 tgagctttac gctggtcgat atcaatctgc aactgcggac tgtcatcgag gccgttatgg
    25981 cgcacacggg ttagctccgg gttttccgcc gccagcgcca gcaactgatt acgtgctgcc
    26041 atcagcgcat cgtgacccgc tccagcgtgg tcctgcaact ccatatcaaa atctgcagaa
    26101 ctaccaagac cgctaattgc tggtgggctg ctggcgataa cgcgagcttc tttaatttgg
    26161 ttaaacgcct tcgttgcacg ctcgataatg gcaaacgagg tgccggtctt actgtcgcgt
    26221 tcgctccagt ctttcaggcg gataaacatt ctcgccacgt tttgcccgtt acccccaggg
    26281 ccagaaccaa cggtggcaaa caccgacatg atgttgtctt tttcatggat gaagtaatat
    26341 ttctcgattt gctcaacgac tttcagggtc tgttgttggg ttgaaccgct gggcaattgt
    26401 accgaggtag taaacatgcc ccggtcttcc agcggtaaga acgacgtcgg caaacgcagg
    26461 aacaggaaca ccatgccgcc aagcagcagg acataaatca caatccagcg caggctacgg
    26521 tggagaattt tcgccacccc tttttcgtag cgttcggcgt tgcggttaaa catctggtta
    26581 aaccaggcaa aaaaaccttt ttgcccgtga tgctcagctt ttttcagtgg cttaagtagc
    26641 gtggcacaca gagccggagt gaggatcatc gctaccagta ctgacagcac catcgccgca
    26701 acaatggtaa tagagaactg gcgatagatg gcaccggtgg tgccgccgaa gaaggccatt
    26761 ggtacaaata ccgccgaaag caccatcgca atcccgacca gtgccccctg gatctgcccc
    26821 atcgatttac gtgtggcttc gcgaggagtg agtccttcct cactcataat acgttcgacg
    26881 ttttccacca ccacgatggc gtcatccacc agcagaccga tcgccagcac catcgcgaac
    26941 atggttaagg tgttgacgct gtaaccgaag gcgtaaagta cggagaaggt tcccatcaac
    27001 accaccggca cggcgatagt gggtatcagc gtggcgcgga agttttgcag gaacagatac
    27061 ataacgagga aaaccagagc gatagcttcc agcagcgttt tcaccacgtc ttcaatggag
    27121 gctttaacaa acgaggtggt ttcatacgcc accttgtatt ccagtccgtg cgggaaatac
    27181 tgcgccagct cgtcgagacg attgagcacc agctccgctg tcgccatttc gtttgcgccg
    27241 gaggccagtt ttacccccaa cccggaggct ggcttaccat tgaagcggct aagataatcg
    27301 tatttctccg cccccatttc gacggtggcg acatcgccca gccttacctc tgagccgtcc
    27361 tgattcaccc gcaaggtgat atcgcggaac tgttctggtg tttgcagcag tgactgggcg
    27421 ttaatggcgg cgttgagcgc ctgcttatcg acggaaggtg taccaccaag ttgcccaacc
    27481 gcaatctgcg cgttctgtga ctcaatggca tcagtgacat ctttagccgt catctggaaa
    27541 ctgttgagtt tcgccgggtc cagccagata cgcatggaat attgcgaacc ataggcatcg
    27601 atatccccga cgccattcac gcggcttaac gggtcctgaa tattactggc aacataatca
    27661 gcaatatcct gtttatccat cgagccatcg gtagagacga aggcaatggt cagaatgttg
    27721 gtatcgccgg ttttacgcac cgtcacgccc tgattttgca ccgcctgcgg taactttcgc
    27781 atggctgatt gcagctggtt ttgtacttgc tgcacggctt cgtccggatc ggtgcctgct
    27841 ttaaaactta aagtgacaga tgcctgaccg gtgccactgc tctgagatga catatacatc
    27901 agattatcga ggccggtcat attttgctcg ataacctggg tcacggtgtt ttccagcgtc
    27961 tgggccgatg cgccgggata gttagcggtc actcgcacat tcggtggcgc gagatcgggg
    28021 tattgttcaa cgggcaatga aaaaatcgcc agggtacctg tcagacacaa caggattgcc
    28081 agcacccagg caaaaatggg gcgatcaata aagaaattcg ccattaaaag aggacctcgt
    28141 gtttctgcgt attgttgtag gcttgacttg cttcactgta gcggcaaggt acgagccaat
    28201 cgtggagaaa aaaaggagtt aatgtaaatt atcatgagat tgctgacaca ctcgcttaca
    28261 ggtcaatatg ggcacgtttt tccctctcgc ctttaaaggg agagggccgg tgtgagggta
    28321 attatttaca cctcagccgg cgtgttcccc ttccccttcc cctcaccctc tgcggagcga
    28381 aagctaatac tcactaacgt gccgccgccg gaaggttgtg aaaaagtcag cgtcccgccc
    28441 agccgttccg cgcgttcgcg catgatattc agtccataat gaccttcggg ttctttcggt
    28501 tcaccgatac caatcccgtt atcacggata taaaccgtgt gattgccgtc cggcgcggtg
    28561 acgcaactga tggcgatttc gctggcgttg gcgtgcttca tcgcattcag caccgcttcg
    28621 cgaataattt gcaacaaatg cacctgcatt tgcgcatcca gtgccagggt tggcagacgg
    28681 cagtcgaggg tcagtttagc gctggtttga ttttgtaacg tatccagcat ttccctcaac
    28741 gcggagggga gatccgcctg ctgcagcgtc aggcggaaag tagtcaacag ctcgcgtaac
    28801 tgccgataag catcattcaa cgcctgggaa aaatcggcca tgatactttg tgcggtggcg
    28861 ttatcttccg gtatcgaacg cttcagtaac gtcaactgga tacgtaagta agaaagcacc
    28921 tgagccagcg agtcgtgcaa ttcgcgggcg atggtcgcac gttcttccat caacaataac
    28981 tgctgaaaat gcttctgcgc ctgattaaag tacaaaccgc gtcccagcat cgatgaaaca
    29041 ctgttaagca gcggttctga tgatgaaacg tgactatttt gccagtgcag ttcgccgtaa
    29101 accgtctctt gcattgtcac cggtaaaatc tgcattggct gctcagggtt tggcttccct
    29161 tcgctaatcc gccagttatc cccaacgttc agctccagat attcagctgc ttcattgtcg
    29221 cgaacaatct gcaaaatatg gcggaaacaa tgcacatcaa tctggctggt gttcagcgcc
    29281 tgggaacact gcactgatac aacacctcca gacgacgctt ggcctcgtgg agatcgcggg
    29341 tcttttcttc tactgacgcc tccagcgaaa ggtacaattt atgcagctcg ctcgacattt
    29401 ggttaaaggt ttttgcaagc agaccaagct cattcggcag actggtatcc agcggcggcg
    29461 agtcgaactg cccgtgttca atacgctgac tggcggtaac cagctgattc agcggggcaa
    29521 ccacctgatg gcgtatgcgg cgcaaagtaa aaaagaccag cgtgaaaata ccgataccgc
    29581 cagccaggga aatcgccacc accagcagca ttttgcgttc agcgtagtgc tgtaaagcca
    29641 gcacgaacag gtctatctga ttaacataat tattaatatt ggcctgatac cacggcaaat
    29701 cgcccttgct gagccgatta ttcatctcca gccagttggc attcagatgc gcatagcgag
    29761 tttttactgc ttctggcaca taccagacgt tgaggttggt taataccggt gaatgcagtg
    29821 cctgctgaaa caactggcga tgtgcattga gttgtggact gccactttgc aagtcgtagc
    29881 ccaggcggta actctgcata cgcagcgatc cggcaatatt gatagcctca gcgtcgcgca
    29941 aactgctcgc cagagttagc agagcgatac ccgtggaaag aatcgacagc agcacaatgt
    30001 aaaaaaaggc ccgggccaga ctggccgaga cggggcgttt aacaatcaca cgcgtcttct
    30061 ccacaaaaaa tcattaatgt tccagttcgc tgaaacgcaa tttagtcagt gatgggatta
    30121 tttgatctgc cacagtttat agcgaaatag ttacttattc tgggcacatg aacattgccg
    30181 gttcccctgt aagtcgggta ataacaacac tcatataaag aataaggttt ttacaaccaa
    30241 aaaagaaggt cgttatgaat cgttttatta tggccaacag tcagcaatgt ctgggttgtc
    30301 atgcttgtga aatcgcctgt gtcatggctc acaatgatga gcaacatgtc ctgagccaac
    30361 accattttca tccccgaatt acggttatca aacatcagca gcaacgtagt gcagtgacct
    30421 gtcaccattg tgaagatgcg ccctgcgccc gtagctgccc taatggcgca atcagccacg
    30481 ttgatgacag cattcaggtc aatcagcaaa agtgtattgg ctgtaaatcc tgcgtggtgg
    30541 cctgtccgtt tggtacgatg caaatcgtcc tgacacccgt cgcggcagga aaagtaaaag
    30601 ccacggcgca taaatgcgac ctttgtgcag ggcgcgaaaa cggtcctgcc tgtgtcgaga
    30661 attgcccggc ggacgcgctg caactggtca ctgacgtcgc actctccggc atggcgaaat
    30721 cccgccgctt gcgcaccgca cgtcaggagc atcaaccgtg gcatgccagt accgctgcac
    30781 aagaaatgcc ggtaatgagt aaagtcgaac aaatgcaggc aacgcccgcg cgtggcgagc
    30841 cggataaact ggcgattgaa gcgcgcaaaa ccggttttga tgaaatttat ctgccatttc
    30901 gcgccgacca ggcacaacgg gaagcctcgc gctgccttaa gttcggcgag cacagcgttt
    30961 gtgaatggac ctgcccgctg cataaccata ttccgcagtg gattgaactg gtgaaagccg
    31021 gaaacatcga cgccgccgtc gagctttctc accagaccaa caccctgccg gaaattaccg
    31081 gacgcgtttg tccgcaagac cgtttgtgtg aaggtgcctg tactattcgc gatgagcacg
    31141 gcgcggtaac tatcggcaac attgaacgct atatttcaga tcaggcgttg gcgaaaggtt
    31201 ggcgtcctga cttaagtcat gtcaccaaag tggacaagcg ggtggcgatt atcggggcag
    31261 gtccggcagg gctggcctgt gcggatgttc tgacccgcaa tggcgtggcg gtgacggtgt
    31321 acgatcgcca tccagaaatc ggtggcttgc tcactttcgg cattccttct ttcaaactgg
    31381 ataaatccct gctggcacgc cgtcgggaaa tcttcagcgc gatggggatt cacttcgaac
    31441 tcaattgtga agtgggtaaa gatgtctctt tggattcgct tttggaacaa tacgacgcgg
    31501 tcttcgttgg cgtaggcact taccgttcca tgaaagcggg tttacccaat gaagatacgc
    31561 cgggcgttta tgacgcgctg ccgttcatca ttgccaacac taaacaggtg atggggctcg
    31621 aagagctacc ggaagagccg tttatcaata ccgccggact taacgtcgtg gtactgggcg
    31681 gcggcgacac cgcgatggac tgtgtgcgta ctgcgctgcg ccacggcgcg agtaacgtca
    31741 cctgcgctta tcgtcgtgat gaagctaaca tgccaggctc gaagaaagag gtgaagaacg
    31801 cccgcgaaga gggggccaac ttcgaattta acgtccagcc ggtggcgctt gagctgaatg
    31861 agcaaggtca cgtttgcgga attcgtttcc tgcgcacgcg tcttggcgag ccggatgccc
    31921 aggggcgtcg gcgacctgta ccgattgagg gcagtgaatt tgtcatgcca gccgatgcgg
    31981 tgattatggc gtttggcttc aatccacacg ggatgccgtg gctggagtcg cacggtgtaa
    32041 cggtagacaa atggggccgc atcatcgcgg atgtggaaag ccagtaccgt taccagacca
    32101 ccaatccgaa aatcttcgcc ggtggcgacg ccgtgcgtgg tgcggatctg gtggttaccg
    32161 caatggcaga aggacgtcat gcggcacagg ggattattga ctggctgggg gtaaaatcag
    32221 tcaaatctca ctgatagcct gcgcagacaa acccgacttc acagcgtaat ataattgttc
    32281 atttcgcgct gtggagtcgg tatgacgcaa caaatcaccc tcattaaaga caaaattctc
    32341 tccgataact atttcaccct gcacaacatt acttacgatc tcacgcgcaa agatggtgaa
    32401 gttatccgcc ataaacgtga agtttacgat cgcggtaatg gtgcgacgat cctcctgtac
    32461 aacgcgaaga aaaagagcgt ggttctgatt cgtcagttcc gtgtcgctac ctgggttaat
    32521 ggcaatgaaa gcgggcagct gattgaaacc tgcgccgggc tgctggataa cgacgaaccg
    32581 gaagtgtgca ttcgtaaaga agcgattgaa gagacgggct atgaagtggg cgaagtgcgc
    32641 aaattatttg aactgtatat gtcgcctggc ggtgtgactg agctaatcca cttttttatc
    32701 gccgaataca gtgacaatca gcgcgctaac gccggtggcg gtgttgaaga tgaagatatt
    32761 gaagtgctcg agctgccgtt cagccaggcg ctggagatga tcaaaaccgg cgagatacgt
    32821 gacggtaaga cggtgttatt gcttaactat ttgcaaatgt cacatttaat ggactgaaaa
    32881 atagcaataa tatttcgttg tttattattg gctcaatccg acaaatccga ttgagccgcg
    32941 ctacctgcgc aacgaagata cgacttgtgc tgtttgtttg aacttctggg gtcgtaccgt
    33001 ccatgcgcta tcgcattttc cttctctttt ttttcgcttt gttgccgacg tctttggtgt
    33061 gggcggcacc agcgcaacgg gcgttttccg actggcaggt cacctgtaat aatcaaaatt
    33121 tttgtgtggc gcgcaatacg ggcgatcata atggactggt gatgaccctg agccgcagcg
    33181 ctggggcgca taccgatgcc gttttacgta ttgagcgcgg tgggttgaag tcgccggatg
    33241 cgtctgaagg ggagatagcg ccacggctgc tattagatgg tgagccgttg gcattaagtg
    33301 gtgacaagtg gcggatttca ccatggttat tagtaaccga tgatacggca accatcaccg
    33361 cgtttttgca gataattcag gaagggaagg cgatcacctt acgcgacggc aatcagacca
    33421 tttctctgag cggcttaaaa gcggcgttgt tgtttattga tgctcagcaa aagcgcgttg
    33481 gcagtgaaac cgcgtggatc aagaaagggg acgaaccgcc tctcagcgta ccgcccgcgc
    33541 ctgcgctgaa agaggtcgcg gtggttaacc caacgccgac gccactctca ctcgaagaac
    33601 gtaacgattt gctggattat ggaaactggc ggatgaatgg tctgcgctgc tcgcttgatc
    33661 cgttacgtcg tgaggtgaat gtcactgcgc tgaccgatga taaagcactg atgatgatta
    33721 gctgtgaggc aggggcctat aacaccattg atttggcatg gattgtgtcg cgtaaaaagc
    33781 cgctagcctc gcgcccggtt cggttgcgtt tgccgttcaa caacggtcag gagacgaatg
    33841 aactggaact gatgaacgca acatttgatg agaaatcgcg tgaactggtg accttagcga
    33901 aagggcgcgg attaagcgat tgtggcattc aggcgcgctg gcgctttgac ggtcagagtt
    33961 ttcgcctggt gcgttacgcc gcagaaccta cctgcgataa ctggcatggg ccagacgcca
    34021 ggcccacgtt gtgatcaccc gttaaacaaa atcgcagatg cggccctaaa aagcgcacgg
    34081 accgtcccct cgcccttctg gggagagggt tagggtgagg ggaacataca gcatccggcg
    34141 atcaccatca ggcacctttc actcccagca ccttatgcgc ttttgccaca atattctcgg
    34201 cggtaaagcc aaagaacggg aacagcttat ccgccggagc agattccccg taacccgtca
    34261 tcccgacaat tgcccctttc agaccaacat acttgtacca gtaatcggca atacctgctt
    34321 ccaccgccac gcgagccgca acgttagaag gcaacaccga ctcccgatat tcctcatcct
    34381 gggcgtcgaa aatatcggtc gagggcaggg aaactacgcg tacattgcga ccttctcctg
    34441 ctaatttctc tgccgcttgc agggtaattt ccatctctga accggtggca atcagaataa
    34501 tatctggctt accgccgctg tctttcagca cataaccgcc acgagcaatc tctttaacct
    34561 gatccggcgt acgttccacc tgggccagat tctgccttga gaggatcagt gccgtcggtc
    34621 cgttgtggcg ctcaaccgcc agcttccagc ccaccgccgc ttccacctga tcgcacggtc
    34681 gccaggtgct gaaatttggc gttaagcgca ggctggccag ttgctcaaca gcctggtgcg
    34741 tcggaccatc ttcgcccagg ccaattgagt cgtgggtata aaccataatc tgccgcgctt
    34801 tcatcagtgc cgccatccgc gcggcgttac gggcgtattc aacaaacatc aggaacgtcg
    34861 cggtatacgg cacaaagccg ccgtggtgcg cgatgccgtt ggcgatagcg gtcatgccaa
    34921 attcacgcac cccgtagtga atgtagttgc ccgccggatc ttccttcagc gaaacagaac
    34981 ctttccagat ggtcaggttg ctgggagcca gatccgccga accgccgagc aactcaggca
    35041 gcatcggccc gtaagcgtta agcgtatttt gcgaagcctt acgggtagcg attttcgccg
    35101 gatttgcctg taactcattg atatatttct gagtcgtttt ctcccagtcc ttcggtaaac
    35161 caccgctcat ccgtcgggta aactcttctg ccagttgcgg atgagccttt ttataggcgg
    35221 caaacttctc attccagctc tgctgcgctt tttcgccttt ttcacgggca tcccaggcgt
    35281 gataaatctc tttagggatc tcaaatggcg gatggtgcca gcccagtttt tgccgtgcca
    35341 gcgccacttc ttcttccccc agtggtgcgc cgtgcgcctc ttccttacct gctttattcg
    35401 gcgaaccaaa gccaatcacc gtacggcaga taatcagcga cggcttatct ttcacgcttt
    35461 gcgcttcaag gatcgcttcc ttcaccgcct gcggatcgtg accgtcgatt tcatggatca
    35521 catgccagtg ataggcttca aaacgttttg ccgtatcgtc ggtaaaccag ccttctgttt
    35581 caccgtcgat ggaaataccg ttgtgatcgt aaaaaccaat cagcttgcct agtcccagcg
    35641 tgcctgccag cgaacagact tcgtgggaaa taccttccat caggcagccg tcgcccataa
    35701 acacataggt gaagtgatcg acgatctcat ggtctggctg gttaaactgc gccgccagtg
    35761 tacgctctgc tatcgccagc ccgacggcgt tcgccaaacc ttgtccaagc gggccggtgg
    35821 tggtttcaac gcctggcgta tagccaatct ccgggtggcc tggggttttc gaatgcaact
    35881 gacggaagtt cttcagttct tccagcggca ggtcgtaacc ggtcagatgt agcaaactgt
    35941 agagcagcat cgacgcgtga ccgttggaaa gaataaagcg gtcgcgatca taccaggttg
    36001 ggtcggtagg attatgttta agaaaatcgt tccacagcac ttcggcaata tcagccatgc
    36061 ccatcggcgc gccgggatga ccagaattgg ctttttgtac cgcatccata ctgagtgcgc
    36121 gaatcgcatt ggcaaggtct tttcgggaca tataacactc cgtggctggt ttatagtttg
    36181 gcggcaagaa gatcttccag tttgcgttga tcaacagcga acagacgaat gccttcagac
    36241 agtttttcta ccgccatcgc atcctgattg tgctcccaac ggaactccgc ttcgctcatg
    36301 ggtgctgggc gtgggaacgt ctgagaaggc gggattaatt tacgtaccac tggagaaact
    36361 ttttcctgca gctccttcag caaattcggt gcgatagtca gtcgatcgca gccggttaag
    36421 gcgaggattt gttcggtgcg acggaagctc gcgcccatca caatggtttc atagtggtgt
    36481 tgcttatagt agtcgtagat attgcgcacc gatttaacgc ccggatcttc ttccaccaca
    36541 tacgggtcca tcggcttgcg tgcctgatac cagtcataaa tacgccctac aaacggcgaa
    36601 atcagaaaaa cgcctgcttc cgcacaggcc cgcgcctggg caaaagaaaa cagcagcgtc
    36661 aggttgcagt taataccttc tttttccagc tcttcggccg cgcgaattcc ttcccaggtc
    36721 gaagccagtt tgatcaaaat gcgtgatttc tcaacgcctt gttgctgata caagtccacc
    36781 agatggcgcg ccttctcaat actcttttct ttatcaaaag agaggcgtgc atcaacttct
    36841 gttgacacgc aaccgggtac gattttgaga atttcagcac cgaaattgac cgccagtttg
    36901 tcacacgctg cgaccacctg ttgttcctgg gtcttgccat tttttttacc ccaggcgata
    36961 gcatcgtcta ttaaatgctc atattgtgat aatccggcag ccttgagtaa cagcgaagga
    37021 ttggtggtgg catccagggg atgataatgg cgaatggact caatatcgcc gctgtctgcc
    37081 acgacagtgg tgaactgttt gatgccgtct aactcgttca taggaaatac tccttgaaaa
    37141 gtaaagtgtt agatgagtgc gttaattcac acttctgaga aatttcgcta aacgcatcaa
    37201 aaaagcatag cagacaggca tggtattgct ggattaagca ggtaatatca gtgttatagg
    37261 attgttacca aaacattata ttaatggtga gagtttggac ttgctcaaag tctgtagact
    37321 ccggcagggt aataatgtgc gccacgttgt gggcaggggc tgttgcccac acactttatt
    37381 tgtgaacgtt acgtgaaagg aacaaccaaa tggatgacca gttaaaacaa agtgcacttg
    37441 atttccatga atttccagtt ccagggaaaa tccaggtttc tccaaccaag cctctggcaa
    37501 cgcagcgcga tctggcgctg gcctactcac caggcgttgc cgcaccttgt cttgaaatcg
    37561 aaaaagaccc gctaaaagcc tacaaatata ccgcgcgcgg taacctggtt gcggtgatct
    37621 ctaacggtac ggcggtgctg gggttaggca acattggcgc gctggcaggc aaaccggtga
    37681 tggaaggcaa gggcgttcta tttaagaaat tcgctgggat tgatgtattt gacattgaag
    37741 ttgatgaact cgacccggac aaatttattg aagtcgtcgc cgcgctcgaa ccgaccttcg
    37801 gcggcatcaa cctcgaagac attaaagcgc cagaatgttt ctatattgaa cagaaactgc
    37861 gcgagcggat gaatattccg gtattccacg acgatcagca cggcacggca atcatcagca
    37921 ctgccgccat cctcaacggc ttgcgcgtgg tggagaaaaa catctccgac gtgcggatgg
    37981 tggtttccgg cgcgggtgcc gcagcaatcg cctgtatgaa cctgctggta gcgctggggc
    38041 tgcaaaaaca taacatcgtg gtttgcgatt caaaaggcgt tatctatcag gggcgtgagc
    38101 caaatatggc ggaaaccaaa gccgcatatg cggtggtgga tgacggcaaa cgtaccctcg
    38161 atgatgtgat tgaaggtgcg gatattttcc tgggctgttc cggcccgaaa gtgctgaccc
    38221 aggaaatggt gaagaaaatg gctcgggcac caatgatcct ggcgctggcg aacccggaac
    38281 cggaaattct gccgccgctg gcgaaagaag tgcgtccgga tgccatcatc tgtaccggcc
    38341 gttccgacta cccgaaccag gtgaacaacg ttctttgctt cccgttcatc ttccgtggcg
    38401 cgcttgacgt tggcgcaacc gccatcaacg aagagatgaa actggcggcg gtacgcgcga
    38461 ttgcagaact ggcccatgca gaacagagtg aagtggtggc ttcagcgtat ggcgttcagg
    38521 atctgagctt tggtccggaa tacatcattc caaaaccgtt tgatccgcgc ttgatcgtta
    38581 agatcgctcc tgcggtcgca aaagcggcga tggagtcggg cgtggcgacg cgtccgattg
    38641 ctgatttcga cgtctacatc gacaagctga ctgagttcgt ttacaaaacc aacctgttta
    38701 tgaagccgat tttctcccag gctcgcaaag cgccgaagcg cgtagttctg ccggaagggg
    38761 aagaggcgcg cgttctgcat gcgactcagg aactggtaac gctgggactg gcgaaaccga
    38821 tccttatcgg tcgtccgaac gtgatcgaaa tgcgcattca gaaactgggc ttgcagatca
    38881 aagcgggcgt tgattttgaa atcgtcaata acgaatccga tccgcgcttt aaagagtact
    38941 ggaccgaata cttccagatc atgaagcgtc gcggtgtcac tcaggaacag gcgcagcgtg
    39001 cgctgatcag taacccgaca gtgatcggcg cgatcatggt tcagcgtggg gaagccgatg
    39061 caatgatctg cggtacggtg ggcgattatc atgaacattt tagcgtggtg aaaaatgtct
    39121 ttggttatcg cgatggcgtt cacaccgcag gtgcaatgaa cgcgctgctg ctgccgagtg
    39181 gtaacacctt tgttgccgat acctacgtca atgacgaacc agacgcagaa gagctggcgg
    39241 agatcacctt gatggcggca gaaactgtcc gtcgttttgg tattgagccg cgcgttgctc
    39301 tgttgtcgca ctccaacttt ggttcttctg actgcccgtc gtcgagcaaa atgcgtcagg
    39361 cgctggaact ggtcagggat cgtgcgccag atctgatgat tgatggtgaa atgcacggcg
    39421 atgcagcgct ggtggaagcg attcgcaacg accgtatgcc ggacagccct ttgaaaggtt
    39481 ctgccaatat tctggtgatg ccgaacatgg aagctgcccg cattagttac aacttactgc
    39541 gcgtttccag ctcggaaggt gtgactgtcg gtccggtgct gatgggcgtg gcgaaaccgg
    39601 ttcacgtgtt aacgccgatc gcatcggtgc gtcgtatcgt caatatggtg gcgctggccg
    39661 tggttgaagc gcaaacccaa ccgctgtaat tcattcttaa ctctcacgct taccctgaat
    39721 attcagggta agcggttttc ctgaaatata ttagcaaagc ttattactga gtttgcgaat
    39781 aataaaaaaa tcagtatata aaatatctcg atattatttt atttatattc atgcgtcgca
    39841 tatgaaagtt tattcataac agtaaatatc tctccattct tagtgatcta cctcaccttt
    39901 taaacgcgct tgccgaattt tgttatttac tctgacgaaa aattgtcacg atacacgaaa
    39961 gtttttcaca ggcggcgact catggataaa gaacgcatca ttcaggaatt tgtgcccggc
    40021 aaacaggtca cactggcgca tctcatcgcg catcccggtg aggaactggc gaagaaaatc
    40081 ggcgttccgg atgcgggcgc aattggcatt atgacgctaa ctcccggcga aacggcgatg
    40141 atcgccggtg atttagcgct gaaagccgct gatgtgcata tcggttttct cgaccgcttc
    40201 agcggtgcat tggtgattta tggctcggtg ggcgcagtag aagaagcgtt atcacaaacc
    40261 gtcagcggtt taggtcgctt attaaattat acgctttgtg aaatgaccaa aagttaatac
    40321 gaggcattca tgaaacgaat tgcgtttgtg ggtagcgtcg gggcaggaaa aacaacgtta
    40381 tttaatgcgt tgcaggggaa ttataccctc gccagaaaaa cacaggccgt ggaatttaat
    40441 gataagggcg atattgatac gccgggtgaa tattttagcc atccccgctg gtatcacgcc
    40501 ttaattacca cgctacagga tgtagacatg ctgatctatg tccatggcgc aaatgacccg
    40561 gaaagtcggt tgcctgccgg gttgctggat attggcgtca gcaaacggca aatcgccgtc
    40621 atcagtaaaa cggacatgcc agatgctgac gtcgccgcaa cgcgaaagtt attgcttgag
    40681 acgggtttcg aagagccgat tttcgagcta aacagccacg acccgcaaag cgtgcagcaa
    40741 ctggtggatt atctggcatc tctcaccaaa caggaggaag caggtgaaaa aactcatcac
    40801 agcgaatgat attcgtgaag cacacgcgcg cggcgaacag gcaatgtccg tggttctgcg
    40861 cgccagcatt attaccccgg aagcccgtga agtgtcggat ctactgggtt ttaccatcac
    40921 cgaatgtgat gaatcgatcc cggtaacggc gtccgtgccc gccagcgtgc cagccgacaa
    40981 aaccgaaagc cagcgcatcc gcgaaaccat cattgcccag ctgccggaag gccagtttac
    41041 cgaaagcctg gtcgcacagc tgatggaaaa agtgatgaag gaaaaacagt cgctgggcga
    41101 ttttgtcgtc gccagcgccg ggccgcacct cgaatccgtg atcgccggtc acggagccgg
    41161 ggcgcaaacc ctttctgaac aacggctgtg tcgggtactg aatatcgata tcggcggtgg
    41221 caccgcgaac tacgccctgt tcgatgccgg aaaaatcagc ggcactgcct gtctcaacgt
    41281 cggtggtcgc ctgctggaaa ccgacagcca ggggcgcgtg gtttacgctc ataaaccggg
    41341 gcagatgatt gtggatgagt gcttcggtgc aggcactgac gcccgttcgc tgaccggcgc
    41401 gcagctggtg caggttaccc ggcggatggc ggcgctgatt gtcgaagtga ttgacggaac
    41461 gctttcgccg ctcgcgcagg cattgatgca aaccggtttg ctgcccgcag gtgttacgcc
    41521 cgaaatcatt acgctttctg gaggtgtggg cgaatgttat cgccaccagc ccgccgaccc
    41581 attctgtttt gccgatattg gcccacttct ggcaacggcg ctgcatgacc atccgcgcct
    41641 gcgtgagatg aatgtgcagt ttccggcgca aaccgtatgc gccacggtga ttggcgcggg
    41701 tgcacatacc ctttcgctct ctggcagcac aatctggctg gagggcgtac aactgccgct
    41761 gcgcaatttg ccggtggcga tcccgattga tgaaacggat ctggtgagtg cctggcaaca
    41821 ggcgctgctt cagctggatc ttgatcccaa aactgacgtg tacgtgctgg cgcttcccgc
    41881 ctcgctgcct gtgcgttacg ccgcggtact gacggtcatc aacgcgctgg tcgatttcgt
    41941 cgcgcgtttt ccgaatccgc atcccctgct ggtggtggcc gggcaggact ttggtaaagc
    42001 tctgggcatg ttgttgcgcc cacagctaca acaactcccg ttggcagtca ttgacgaagt
    42061 gattgtccgc gcgggggact atatcgacat tggtacgcct ctttttggcg gatcggttgt
    42121 gccggtgacg gtgaaatcac tcgcatttcc ttcctgaata cggtagttat caggtatggt
    42181 gcttggtggc tgggtgttag tgggattata tccggcagtg tatccggtta aggtgattgg
    42241 tctggcaaga tgattggatg cttttgtggt gaaaaatgtt ttggtgtttt agcataaccc
    42301 tgaagaaaga ataaaaataa aaaggaaaaa accaaaacca gaacagaaga gaaccggaaa
    42361 gagaagaaaa gagaaaatga aaaataaaaa ccaaaaaaga aagaatactt aaatcttcgc
    42421 atgttcttcg ggtggtacgc aagtttgcta gtctgactgc acaataaatt ttcgcttaga
    42481 aaataataag ttacataata ttctgctttt ttagataaat ataacccgaa aaaatcattg
    42541 ttaatcttat gcatttatta tttgattgta agttgtgatt attataattt agttattgtt
    42601 agtatgtttt ataatgctgg atgggtgatg cctctaatta gttgaatctg atgtataata
    42661 cgggcttttg aggttatctc atgaccagcg ttaatattca ttgtccccgt tgtcagtcag
    42721 ctcaggttta ccgccatggt cagaacccta aaggccgtga cagacttcgc tgccgtgact
    42781 gccaccgtgt gtttcagttc acttatactt atcaagcacg taagccgggt atgaaagagc
    42841 tgataactga aatggccttt aatggtgccg gggttcgcga taccgccagg acactgaaaa
    42901 ttggtagtaa caccgtcatc cggactttaa aaaactcgcg ccaaagcgaa taacttcttc
    42961 gcctgttgcc catgctgatg tggcgtttat ctgcgagctt gatgagcaat ggagctacgt
    43021 tggcagtaaa gcccggcaac attggctcgg gtacgcgtac aacaccaaaa caggcggtgt
    43081 actggcctac acttttggtc cccgaaccga tcaaacgtgc cgggagctac tggcactgcc
    43141 acccttcaac atcggcatgc tgaccagcga tgactggggc agctatggcc gggaggtgcc
    43201 gaagaataag catctgaccg gcaaaatatt cacccaacgc attgagcgta ataatctgac
    43261 gctacgcacc cgcattaagc ggttgggtcg taaaacaatc tgcttctcac gctcagttga
    43321 gatccacgaa aaagtgatcg gagcgtttat cgaaaaacac atgttctact aattggatgc
    43381 actacccagt atgtaactgg gtttgtccat aaaatgcacc ttactcagtt ggtgtccaac
    43441 ttttggggtg cagttcagaa ggacgggatt ttacggtgca ccggataaat ttaaaactgc
    43501 ctgatactga atgtatgagg tttcgtaatg ctcaattccc ctagtaccgt tttgtagatc
    43561 tccatacttc cactagtatt tatttgcaaa gttcctatat ttgaggagtt taataaattt
    43621 agctgtccca agtgattttt aatagagata gcctgatcat gtgcaggatt aataaatttt
    43681 atatccggtg caactgcttc aaaaaaattc tgaacaattg gatagtgagt acagccgaga
    43741 ataatttcat ttactggatg gagggaaatt aaatggttaa catgtttttt gatgtcattc
    43801 aagattgcag gagcattaag attaccttcc tcaattaaca ttgcaagatt tttgcttggt
    43861 tctccaaata catttattgt tgaattcaat ttttttatta gctctttgtg acaccccgtt
    43921 ttaatagtaa actcggtagc gataatacca acatttttat attgctggcg aatcacatct
    43981 tctactgcag gttcaatgat ggataagata ggaaacggaa agctcttagc atatccgctg
    44041 tatttttcgg attccaaagt tgaggatata gtattacatg cgacagcaac tagtttgaca
    44101 ttccttgact gtaaaaaagt cagcattttt ttagtcaaac tgatgatatc atcggcagaa
    44161 cggttgccat aaggtgcatt catattatct ccacaataga taatatcttc accaggaagc
    44221 aaattaatta tttcctttac cgatgtcaat ccacctacac ctgagtctat tacaccaatt
    44281 ggagattcag cactaaacat atttaacctc atcttttgta gcttatggaa aatttaatta
    44341 ctagttcttt tttcccaaga aaatggttcg aaatcagagt ttggatcttt ccagtcagat
    44401 tttcggaagg cgtaaattaa tattggaatt aataaaaata gggcaactaa tactaacaaa
    44461 taaagaacat agctggttgg actaccagtt gggatttgct cgggcggaat aaaacttaat
    44521 acaaaagcca gaaaagaccc taagaatcca agtgttgccc agaaaaataa agcaggggct
    44581 cgatatgggc gttttgtatc aggttgagaa tgtcgtagat agatagcact agcgaacatg
    44641 agcatataca taactaaata gagcataact gttaactgtg ataaaatctg aaaagctgct
    44701 tgaactgaag gcattatcac aaaagtaaca gaaagaactg agacaattat accttgtaca
    44761 aacattagat ggtgccccat tccatgacga ttggtttttt ggaaaaactt aggtaaataa
    44821 ccagctttcg cgatcgctaa tacacctgta ttaggccctg caatccaagt aaccacccca
    44881 cccagtacgc caattgctag cataaatgcc atcacagagc caagccatgg cactccagcc
    44941 cattcgaaca gcatatcgta ggcaaccaac aaactttgag ttagactaat tttatctgta
    45001 ggaataataa aggcgacccc taaagtactg agcaggaata ttgcaatagt acctatagaa
    45061 gcgatggtaa ttgcaattgg gtatttttta gttggattat ctaaatcgtt aacatggacc
    45121 gcattcattt ccatccctgc ataagcaaga aatatgctgg cggcaagaac aatattctgg
    45181 aagttagcta tattagggaa aagatcaccc caacctaatg ggatcaccgc tggtttaccg
    45241 ctgaaccaca gataggagaa accaagaata acaaggataa tagctggaat aattgtccct
    45301 ataataccgc accacttaga tacctgcgag aaaataccaa cgccacgtaa tgcaacacaa
    45361 atagctcccc agtaaacaaa tagtacgaaa aataaaatat accatttatt actggcaatt
    45421 gattctgcca cccccatatc aacatcaata aatgcaacag agactgcacc aaatgtcagt
    45481 actgttggaa agtaggggat gacttgtagc caggtcataa gcattcctac aaaaccccag
    45541 cgagccccaa atgcttctcc tatccatcga aatgcccccc ctttttgtgg atatgttgta
    45601 gctagctcag ccgcaacaaa ggcaacaggt actaaaaaca taattgcagc aaaggtatag
    45661 taaaaaatgg cacctaagcc gtattctgct tcagaaggta taaatcgtag acttactaca
    45721 gcagttatat tcatgatagc gagtgtcccc actgtcattt tcttactttt gggtgcgata
    45781 ttagcattag acatttttat actctcgaat atttttaaaa tagaaacata taattacgat
    45841 gtaaatataa aaactaatat taactttaac acttatctta aaaacactaa cgcaatctgc
    45901 tttacaaaaa taagtaatac ctaaagtcgt aacgacacca atagtgtttt catcgacatg
    45961 cttcaaaacc tctttagcat tcattatcag gtgatgatca tcaagtggaa tttcacacaa
    46021 tttcacatca cagtaacggc agaatttatg ccagcatatt tgcacaggat cgcaaatcag
    46081 gttcggttga tttaccttgt acttcgcgtt ttttacacca ctgccatttt aacgccaagc
    46141 ggccgagcat agcagcttca gatgaaccaa tggttgagca acctaaggtg tgtcgcgaat
    46201 cggggaaact ccacgaatcg gaaagcatac gcacacagcg accttcaggt tccgccattt
    46261 gtggatattc atctttataa atcatgtttt tatcaatagc taaattaagc aactcacgga
    46321 tttcatcatc aagccatgtt tgacagaaag tagctaagtt ttgtcgtgaa tccccatcaa
    46381 ggatgagctc atcacgtatg gcgctgaata catttcgaga tttcttctca tctacacgca
    46441 aatgatattt atgcaatata aatgaaagct caagtgagct atgaatatcg actgcttctc
    46501 tataacaaga attacctgat ttatttttca taataaacgc cactcaatac aatcagatta
    46561 aacattacaa acacaatctg gattttgcaa ttttaaaact atcaatttta gatatatttt
    46621 aattaatata aaaccataaa catatcaaaa aattacttta ttatggtcat ggtagatgtg
    46681 tttcaatgcc aacctggtct attcctacat atgtgagagc atatttacaa aaaaatcgtt
    46741 attgtgattt caacggtgat gctgaagtgg cacactaaat ttggccacct gaacagaggt
    46801 gatatgctca cctcagaaca acatgacgtg gtgttattct gctcagtact gttctggcaa
    46861 tgaatccagc cagtcctctg catcttcctt gtaacactcg tagatgcagt aattggtgaa
    46921 actgatgtat ttggttagta actctgcttc gttgttcctg tgcgctcttc tggcctgctt
    46981 acgtagttgt ttcgcttctg cccgctggcc ttcgagtatc tccagcctgt ctgcaatatc
    47041 ttgcttcatg ttcagctcct cgcgcgcgca tagtacttgt ccgaaaatta atcgcccgtc
    47101 ccatcattaa tatcgattcc cacgctgtgt aactggctat gaatgtctct gcgaatgaga
    47161 attaaacgct tgatgaactc catttcctcc gcgctctggt tgctcggccc cgatttcatg
    47221 tcgatgattt tattgatagt tccaagcgaa tattcagccc acagacgaag cgtttagcgc
    47281 ttcatggcct gtttgctcag tggcgaatgt ttcaggtcta agacatgttc tgcgagggtc
    47341 ttagcgatgc cctgcatgat gcgtagtgcc aacatgatga ttttctccgg acttgtgcta
    47401 atatagttcc cgaacattgt ttcgagactg cttatcctgt ttgtcttgtc cgatttgttc
    47461 attggtcaca ttgttaagtc agccggggta attcccggct ttcttttatc cgatcagtac
    47521 cgaatcccta tcgcattgaa tacgcgtttc cgttgctcag cttcaggatc ggtggccatt
    47581 tctggttctg gctccggcat agacggggcg gcatcaaaat gctcaatttc acgaccgaaa
    47641 tacgagttca ggtcaatacc tgccgaacta tccactccat caaaatagcg acgctgttcc
    47701 tcggtgagac agctacccag acgttcctca acctgcgcca taaactcaac atccggtgag
    47761 aagtactcat cgggattcgg agcaccgctc tgtttgtgca tctccatttt ctctgcgttc
    47821 gtttttagcc cggcaatgcg tttctccagt ccggcggctt taatagcgtc agcaacgatt
    47881 tgtgggatct gcggtttaat tgtttcgata acttcattac tgattaattt aaactgggcc
    47941 atacggatcg ctttaatctg tttatttagc agtgcgcgtt ccccttcgtt ggcggtttta
    48001 tcacgctgtg ctaccagttc agcgccttta tttgccagta cctcaaatgc gtccattatc
    48061 tttcccccct tttttcgatt atttaaaagc gcttattgac acgccaaaac gacgacttaa
    48121 ttagcaaatg agtcgccgct ttgcagtgcc ttccatatct tccggcattt cagactgaag
    48181 tttatcagct acttcatcaa caagtggacg aaccgtttcc acgaacttgt ccgcaatata
    48241 atcagcagaa cgcatcactt tctcatggaa ttcctcacgg cgttcctggg cggctgattt
    48301 aggggctgtc gtgcgctgaa aataactgtc taatgaatgc atggtattta ctactgtgtt
    48361 tatagtgatt gttcaattaa ttgcggttga agccattgtg tgtggcccag ccgaataagt
    48421 ttgcggacgt ttttatgtat ccggcggtgc ttttctttcg gcatcaggcg aatattcttg
    48481 atgcggttat cctgtttgtc caaattaacg tggtgcactt catataatgt ctcgtcataa
    48541 gcgccgtatg tgtatacata gcaggctttc tgataggtga gtgtcacttt gaatttacct
    48601 tcatgtaccg ttacaaccaa atgcccttta ttggtcatgc tgcctttgat aacgtctttt
    48661 gtttcacggc taaacaataa acccgcttca gggtcataaa tccatctgcg ccgcataata
    48721 tcggcacagc gttctaatct ttcgatattg gtcatgatgt ttacctcaaa atgtgatgcg
    48781 tgattattat cgttctgttc tgtacaacca ttctttgcat tcgtgctccc agaataattg
    48841 gcgagtaccg cgtggggtaa atggcaaagg gactccggtt gatggttttt ttagccagcg
    48901 cctcagcgtt ctggtcgata cattcgccat tgctgcgaac tgttctaatg ttattgcttt
    48961 cataaatacc tcgatacgaa aaagaagccg ccccacggag gaaagaagaa gcaaattttg
    49021 aatttccgtg aggcggtgga ttccgattcc ctgaacccat tacccatgtg atacttaata
    49081 ttataaacct tattttttct gttgacattt ttggccatat attgacagcg gtttttgtta
    49141 gcaaaaagtg ctatttatta aatcatcaaa gcaataaaag attatttgca cccgcaaggt
    49201 aatggcggga taatattagc gagggttagc cgccctcgac ctcaaagtga tctgttaata
    49261 actacaacga tatgtctcta tttcccgctt ctccggcggg ttttttttgc ctgcaaatca
    49321 ccacatcaga attaattaaa ttgataccgt ttaaaacgct cccaatcgca atataacggc
    49381 cttaacttct ggctatgcat ttgctcaggt gtgatcaaat tgattcgtca ggcgtggaat
    49441 aaggcaataa aaacgggcat tgcgcccgct tgtgttcact gcatcgaaaa taacttatcc
    49501 ataattcttt tctcctattc cctgcacatc atttcggcac ggtcccgatc ttcctgtgtc
    49561 ggattaaagt ccggcgttct gtatctgttg cctgaaatat accgtgcatg attgcggcga
    49621 cctgaacgcg gcattatcca gtattcgatg ttctcacgcg tggcaatgag taaaccatct
    49681 tctacaagcg tattcagggc gtgccatagc tgcttgtaag taacgcaacc ttcaccgaca
    49741 agataatcca gtgcgcacga caatccgctt actgttaact ctttggcggc gaaatttggg
    49801 tgttcagaaa ggaaatactc ctgaccctgt ttgctgaata cccaaaggat ctggcggcgc
    49861 aattcttcaa cgtcctggct ataagcgtga ttgatatgtc tttcagctac gaaagcgcgg
    49921 ccttgtttcc tgattagccc attactaacc atcgtcatga gtgagcggcg ggcggcgtca
    49981 acctggacgc gggtaacatt gccaccataa acgagatctg cggcttgtcg tgcggtgatt
    50041 gattcgtttt ttgccagata gtcgaggatc tgccgttgtt gttctgatat gcgcatgatg
    50101 tgtaaccccc tgattttcac aataaactta tctcttttct gcttaatgta tgtatctatt
    50161 atgcgctcaa tatcgaggcg gggcaatgtt gcgatcagtc ctgatcgcca ccattcaggc
    50221 gagtgagaaa aatttaatga aatcgcatcg attttaagcg ctctcaatcg ctctgtggcg
    50281 acgttaagtt ttcaggtaag caagttagcg cttatgtgtg ttcatcgcgt tctatgggcc
    50341 attcctttcg aatttggcgc ggtcatgata gttcgcggtg atcggcggcg gggataaagt
    50401 ttcctgagtg atcacttaag cctgctgtgc gtcgtgtgag tgtgctgtct gcacctggct
    50461 atacgctgct ttgctgctgt ttcctgtctg gtctgtatac gcgataaaaa ccattggtct
    50521 tgcagtgtgg cggtgtcgtt ggtttttatt gagcgcgtag cgcgatagaa ggtgcgctct
    50581 cagatgttta tgtgtctacc tatggtgagc gctccgtgcc ttacgtcgtt tcattgagca
    50641 ttggccccag gagacgagcg aagcgaagct ctccccactg ctaagctggt tttgttttta
    50701 tcctgcatgc tattccgctt aagccagcta gttaattcgt caaggaatga tttaaagagt
    50761 cctttaacat tatggtcaca atccgaacag ctttatcgtg aggatttgac ctagccgagc
    50821 ggcaatgagc gtagtcctgc tgcacagaag ttaaggtctg ctctggaggt tatcaggacg
    50881 taagcactag tctcatgatt ccgccgaaaa gacgaaaaat cttggcgctg atctgtattt
    50941 attatttctc tgtatttatt actttgggcc gcaaaaatgc ggctagtgag tgggcaaatt
    51001 tgcggctagt gagtgggcaa aaatgcccac ctattttttt tcagcgtcaa aatcatggca
    51061 aaatcatgac ctttctggat gcttcttgaa cagtaatcac gctttttctt gcactaacca
    51121 attgaataat aaaaggttct ggcaaatagg accgcatttt tgcggcttat tttgcttgca
    51181 gccagcgcca cgtctgggtt tgagctatca ctatcaaact ggttgtaacc gcgatgtgat
    51241 tattagttta ttgaggtaat gatctgatta tctgacatga aaaaaggggc atttgccccc
    51301 ctttgctgac ccgctgccta gtgtaccagg tagctgactt ttgcttctct ttcgtgctgc
    51361 tgtgccgtca ttgaggcctc ttctgctgtg ccttttgacg ggattcgcag tctgtaggtt
    51421 tttccacatt tgcccctcag tgcctggcgc atttcctgga taagtcccag ctttctcaac
    51481 gcttccaggt ggcgatctat cgtgtcaacg ctcttaccta acgcttttgc cattttggtg
    51541 cgggaatacc aaatctcata tttgtcgtta ccgcgatacc tgcacttgga taccatccac
    51601 gtatatagct ttaactcatc gcctttcagc ttgtgaaaca gcccgctttt gaagaggtaa
    51661 gccatagcgt tattgacggc tggaaggttg cgataatcgt tttgctggtc ttccgtttcc
    51721 acctgttccc gctcttcagg caactgctcc tctttactat caaaacgcaa atcttcttcc
    51781 gcttcttcgt taaaccatga agtcggcttg tctgtgttgg ctttcgctac tggcttgggc
    51841 attgccagcg ggtggcggca ttcttcttgc tgcgctgtga tttgttgtct ttgcagctcc
    51901 ttgaactcgg caatagccgc cgctttattc tgttcctgtt cctgttcctg ttcctgttcc
    51961 tgttcctgtt cctgttcctg ctcctgctcc tgctcctgct cctgctcctg cgctggttct
    52021 gcattggcta gcgctggctc ttcattggct ggcgacaaga tttgatctat gcgcacgaat
    52081 ccagttgctg gcttgtattc ccagcgatac ccatcgaagc ggatgatgtt catgccatca
    52141 cgaaaggcgg cctgaacctg ttccggaagg gcttggagtg tttgaagtgc ttggagtgtt
    52201 gcaattttgt gtttgaccag tgtttcgcct ggtttcgcca cgtaggcggt tgctgattgg
    52261 tacatggtat ttttttcctg tgttgttttt gtgtccccct agtgtgctgt agcctccagg
    52321 gtgtttttgt gttgatccta ttttcgatcc ccgatccacc ctgttgcaaa cacggcttta
    52381 agtcattgat atttattcat ttattccaac gatactggaa taattggaat aattgatccc
    52441 atcggcaaag acaaaaaaag ccggacgaat ccggaaaaag tgaatagtta ttttgaaggt
    52501 gtccagtgtt tatccggcct actccggtgg agtcgtcgga aaacgtgatt aatcattcat
    52561 tacagttcaa gcggattgat accatcagcc agtatcgaat caatcgcgtc ggcgtagtcc
    52621 tgaagcattt gacggcgcat tttgagatag gccgcttggt tgtaaactcc cctaattccc
    52681 ttcgggacgt gggctagttg gatctcaatc caatcagggt tatacctgcc gctttcgtta
    52741 agtgcggtgc tgaacgttgt gcgccagccg tggcctgtgg ctattgactt gtatccgata
    52801 cgctcaagcg ccttgagtat cgcgctggct gataatggct tatccatgaa tcggcccggg
    52861 aacacgaaaa cactgtatcc gctgcgtgtg aactggtcct gcaatagctc gaccgcctgc
    52921 cgtgatagcg ggaccacatg cgggcggcgg gccttcatga cttcagcggg caatgtgatt
    52981 gttgcgtcct ccaggtttat ccattcccaa cggcacaaac gcagttctgc ggtccttaat
    53041 gccgttaata taagcaaacg tattgccgcc tgaatctgga cgctcccggc cctctcaagg
    53101 gctttgtaga aggccggaat ttcactaatc ggtatccacg cgttgtgacc aggtcggcgg
    53161 gccttcagtg ccgtatcaag atctgcaacc ggattcctcg ttaccagttc ggtgataacg
    53221 gcaaacttga agatctcacc gatgcgctgg cgtagcttgc gggcctgctc cggtgcggta
    53281 cgttctttct tctggagtaa tgccagcacg tcacgggcgg ttatatcaac gattgaacgc
    53341 ttgccaagta ccggataaac gtctttctcc agtgctgatt tgacctgctt tgtatagctc
    53401 tcactccagc cgcctaccct ggtagccagc cagtcctccg ctaccgtctt aaaacctgcc
    53461 gccatgttga gtgcatcaat ggctttctcc ttgcgtgcca aatccattgg gtcaattccc
    53521 tgggctatca gtaaacggct ctctgtggcg cgtttgcggg cttccttgag gctaatcagc
    53581 ggatataccc caatagcctg taaccgctct ttcttgtcta tccagtaccg gaatcgccag
    53641 tatttgctgc cgttgggatg caccagcaaa aacaggccgg gcgtcagtga agcggtgagt
    53701 ttgtattgct tgtcctttgg ttgtgcggcg tctatggcct ttgctgtgag tggtgttttg
    53761 ctcaaaaccg gatacctcga tctaacgact cgataacccc gctcatgtat ccggcgagtt
    53821 cggttgcgtt cggcaggata agtaacgggc gtgttaagga aatcacctta catgacaagg
    53881 gctaacgatg aaatagaatt gtttgcaggc tgtgagaaat gttactcatc acttcttcct
    53941 gagggaacga cttatgaaac taaagaccac attgttcggc aatgtatatc agtttaagga
    54001 tgtaaaagag gtgctggcta aagccaacga actgcgttcg ggggatgtgc tggcgggcgt
    54061 tgcagcggca agctcacagg agcgcgtggc ggcaaagcag gtgttgtcgg aaatgaccgt
    54121 agcggacatc cgcaataatc cggtgattgc ctatgaagat gactgcgtga cgcggctgat
    54181 tcaggacgac gttaacgaaa cggcctacaa ccagattaaa aactggagca tcagcgaact
    54241 gcgtgagtat gtgctgagcg atgaaaccag cgtggacgac attgccttta cccgcaaagg
    54301 gctgacctcg gaagtggtcg cggcgatagc gaagatttgc tccaacgcgg acctgatcta
    54361 cggcgcgaag aaaatgccgg taatcaaaaa ggccaatacc accatcggta ttccgggcac
    54421 ctttagcgcc cgtttgcagc cgaacgatac ccgtgacgac gtgcaaagta tcgctgcgca
    54481 aatctatgaa gggctttcct tcggggtggg cgatgcggtg atcggcgtta acccggtaac
    54541 tgacgacgtg gaaaacttaa gccgcgtgct ggataccatt tatggcgtga tcgacaaatt
    54601 caacatccca actcagggct gcgtactggc gcacgtcacc acccagatcg aagcgattcg
    54661 tcgcggcgct cctggcggac tgattttcca gagtatctgt ggcagcgaaa aagggctgaa
    54721 agagtttggc gtggaactgg cgatgctcga cgaagcgcgc gcagtgggcg cagagttcaa
    54781 tcgtatcgcc ggggaaaact gcctctactt cgaaaccgga caaggctctg cgctatccgc
    54841 tggcgctaac ttcggcgctg accaggtgac gatggaagca cgtaactatg ggctggcgcg
    54901 tcattacgat ccgtttatcg tcaacaccgt ggttggcttt attgggccgg agtatctcta
    54961 caacgaccgc cagattatcc gtgctggctt agaagatcac tttatgggca agctgagcgg
    55021 catctctatg ggctgtgacg gctgctacac caaccacgct gactctgacc agaacctcaa
    55081 cgaaaacctg atgatcctgc tcgccaccgc aggctgcaac tacatcatgg ggatgccgct
    55141 gggtgatgac atcatgctca actaccagac caccgcattc cacgataccg ccactgtgcg
    55201 tcagttactc aacctgcgcc cgtcaccaga gtttgaacgc tggctggaaa gcatgggcat
    55261 tatggcaaac ggtcgcctga ccaaacgggc gggcgatccg tcactgttct tctgatgacg
    55321 cggagataac acatcatgga tcaaaaacag attgaagaaa ttgtacgcag cgtgatggcg
    55381 tcaatgggac aagcggcccc cgcgccgtca gaagcaaagt gcgccaccac caactgtgcg
    55441 gcaccggtga cctcggaaag ctgtgcgctg gatttaggtt ccgctgaagc aaaagcgtgg
    55501 attggtgttg aaaacccgca tcgcgcagac gtattaacag aactgcgccg cagcaccgtg
    55561 gcccgcgttt gtaccggtcg tgccggtccg cgtccacgta ctcaggcgct gctgcgtttc
    55621 ttggccgatc actctcgttc gaaagatacc gtactgaaag aagtgccgga agagtgggtg
    55681 aaagcgcagg gcttgctgga agtacgctct gaaattagcg acaaaaatct ctacctgact
    55741 cgcccggata tgggccgccg cctgtgtgca gaagctgttg aagcgctgaa agcacagtgt
    55801 gttgctaatc cggacgtaca ggtcgttatt tctgatggcc tgtcaactga tgccatcacc
    55861 gtcaactacg aagagatcct gccgccgctg atggcgggcc tgaaacaggc cgggctgaaa
    55921 gtcggcacgc cattctttgt tcgttatggt cgcgtgaaga ttgaagatca gatcggtgag
    55981 atcctcggcg cgaaagtggt gatcctgctg gtgggggaac gtccggggtt agggcagtca
    56041 gaaagcctct cctgctacgc cgtttactcg ccgcgtatgg cgaccaccgt tgaggccgat
    56101 cgcacctgta tctctaacat tcaccagggc ggcacgccgc cggttgaagc cgcagccgtc
    56161 attgtggatt tggcgaaacg tatgctggag cagaaagcat ccggcatcaa catgacccga
    56221 taaggaggca tcatgccagc tttagatttg attcgaccgt cggtaaccgc catgcgggtg
    56281 attgcctccg ttaacgctga atttgcgcgt gaactaaaat tgccgccgca tattcgtagc
    56341 ctcgggctga tttctgccga ttctgacgac gtaacctaca ttgcggctga cgaagcgacc
    56401 aaacaagcga tggtcgaagt ggtatatggt cgctcgctgt atgcgggagc ggcacacggc
    56461 ccgtcaccga ctgccggtga agtgctgatt atgcttggtg ggccaaaccc ggcggaagtg
    56521 cgtgccggtc tggatgcgat ggttgcacat attgaaaatg gcgcagcttt ccagtgggcc
    56581 aatgacgcag aaaacaccgc cttcctggcg catgtagttt cgcgtacagg ttcttatctt
    56641 tcatcaaccg ccgggatcac gcttggcgat ccgatggcgt atctggtggc accgccgctg
    56701 gaagcgacct acggcattga tgccgcgttg aaatctgccg acgtacagct ggtgacctac
    56761 gtcccgccac cgtctgaaac caactactcg gcagcatttt taaccggtag ccaggccgcg
    56821 tgtaaagcag cctgtaacgc ctttaccgat gcagtgctgg aaatcgcgcg taatccaatc
    56881 cagcgtgcgt aacggaggtt gccgatgatc aatgcactgg gattgctgga agtggacggc
    56941 atggtcgccg cgatagatgc agcggatgcc atgctcaaag cagctaacgt tcgtctgctc
    57001 agtcacgaag tgcttgaccc tggtcgctta acgctggtgg tggaaggcga tctggcggcg
    57061 tgtcgtgcgg cgctggacgc aggttgtgct gccgcgatgc gtaccgggcg tgtcatcagc
    57121 cgtaaggaga tcggtcggcc agacgatgac acccag
//
LOCUS       DADLTF010000007        56574 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.007, whole genome shotgun sequence.
ACCESSION   DADLTF010000007 DADLTF010000000
VERSION     DADLTF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 56574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.007"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1017)
                     /gene="setA"
                     /locus_tag="JN749_000454"
     CDS             complement(<1..1017)
                     /gene="setA"
                     /locus_tag="JN749_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HAY7366978.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGA"
     gene            complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="JN749_000455"
     CDS             complement(1119..1250)
                     /gene="sgrT"
                     /locus_tag="JN749_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HAY7366979.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1339..2994
                     /locus_tag="JN749_000456"
     CDS             1339..2994
                     /locus_tag="JN749_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HAY7366980.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3028..3127
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3158..4141
                     /gene="thiB"
                     /locus_tag="JN749_000457"
     CDS             3158..4141
                     /gene="thiB"
                     /locus_tag="JN749_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HAY7366981.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPSGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4117..5727
                     /gene="thiP"
                     /locus_tag="JN749_000458"
     CDS             4117..5727
                     /gene="thiP"
                     /locus_tag="JN749_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HAY7366982.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5711..6409
                     /gene="thiQ"
                     /locus_tag="JN749_000459"
     CDS             5711..6409
                     /gene="thiQ"
                     /locus_tag="JN749_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HAY7366983.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(6523..7287)
                     /locus_tag="JN749_000460"
     CDS             complement(6523..7287)
                     /locus_tag="JN749_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7366984.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7373..8251)
                     /gene="araC"
                     /locus_tag="JN749_000461"
     CDS             complement(7373..8251)
                     /gene="araC"
                     /locus_tag="JN749_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HAY7366985.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8590..10226
                     /gene="araB"
                     /locus_tag="JN749_000462"
                     /pseudo
     CDS             8590..10226
                     /gene="araB"
                     /locus_tag="JN749_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            10237..11739
                     /gene="araA"
                     /locus_tag="JN749_000463"
     CDS             10237..11739
                     /gene="araA"
                     /locus_tag="JN749_000463"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAY7366986.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            11939..12634
                     /gene="araD"
                     /locus_tag="JN749_000464"
     CDS             11939..12634
                     /gene="araD"
                     /locus_tag="JN749_000464"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAY7366987.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12709..15060
                     /gene="polB"
                     /locus_tag="JN749_000465"
     CDS             12709..15060
                     /gene="polB"
                     /locus_tag="JN749_000465"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HAY7366988.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHNPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15224..18130
                     /gene="rapA"
                     /locus_tag="JN749_000466"
     CDS             15224..18130
                     /gene="rapA"
                     /locus_tag="JN749_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HAY7366989.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAILLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18142..18801
                     /gene="rluA"
                     /locus_tag="JN749_000467"
     CDS             18142..18801
                     /gene="rluA"
                     /locus_tag="JN749_000467"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HAY7366990.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="JN749_000468"
     CDS             complement(18918..19733)
                     /gene="djlA"
                     /locus_tag="JN749_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HAY7366991.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            19988..22342
                     /gene="lptD"
                     /locus_tag="JN749_000469"
     CDS             19988..22342
                     /gene="lptD"
                     /locus_tag="JN749_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HAY7366992.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22395..23681
                     /gene="surA"
                     /locus_tag="JN749_000470"
     CDS             22395..23681
                     /gene="surA"
                     /locus_tag="JN749_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HAY7366993.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRANAYVKILSN"
     gene            23681..24670
                     /gene="pdxA"
                     /locus_tag="JN749_000471"
     CDS             23681..24670
                     /gene="pdxA"
                     /locus_tag="JN749_000471"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HAY7366994.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24667..25488
                     /gene="rsmA"
                     /locus_tag="JN749_000472"
     CDS             24667..25488
                     /gene="rsmA"
                     /locus_tag="JN749_000472"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAY7366995.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25491..25868
                     /gene="apaG"
                     /locus_tag="JN749_000473"
     CDS             25491..25868
                     /gene="apaG"
                     /locus_tag="JN749_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HAY7366996.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25875..26717
                     /gene="apaH"
                     /locus_tag="JN749_000474"
     CDS             25875..26717
                     /gene="apaH"
                     /locus_tag="JN749_000474"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HAY7366997.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26795..27274)
                     /gene="folA"
                     /locus_tag="JN749_000475"
     CDS             complement(26795..27274)
                     /gene="folA"
                     /locus_tag="JN749_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HAY7366998.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="JN749_000476"
     CDS             complement(27465..29327)
                     /gene="kefC"
                     /locus_tag="JN749_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HAY7366999.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="JN749_000477"
     CDS             complement(29320..29850)
                     /gene="kefF"
                     /locus_tag="JN749_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HAY7367000.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(29898..31292)
                     /locus_tag="JN749_000478"
     CDS             complement(29898..31292)
                     /locus_tag="JN749_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367001.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="JN749_000479"
     CDS             complement(31351..31638)
                     /gene="fixX"
                     /locus_tag="JN749_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HAY7367002.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31635..32921)
                     /locus_tag="JN749_000480"
     CDS             complement(31635..32921)
                     /locus_tag="JN749_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7367003.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSLSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESSPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(32972..33913)
                     /locus_tag="JN749_000481"
     CDS             complement(32972..33913)
                     /locus_tag="JN749_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7367004.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(33928..34698)
                     /locus_tag="JN749_000482"
     CDS             complement(33928..34698)
                     /locus_tag="JN749_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HAY7367005.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVNA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35169..36683
                     /gene="caiT"
                     /locus_tag="JN749_000483"
     CDS             35169..36683
                     /gene="caiT"
                     /locus_tag="JN749_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HAY7367006.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFDIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36714..37856
                     /gene="caiA"
                     /locus_tag="JN749_000484"
     CDS             36714..37856
                     /gene="caiA"
                     /locus_tag="JN749_000484"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HAY7367007.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            37985..39202
                     /gene="caiB"
                     /locus_tag="JN749_000485"
     CDS             37985..39202
                     /gene="caiB"
                     /locus_tag="JN749_000485"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HAY7367008.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            39276..40829
                     /gene="caiC"
                     /locus_tag="JN749_000486"
     CDS             39276..40829
                     /gene="caiC"
                     /locus_tag="JN749_000486"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HAY7367009.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            40938..41723
                     /gene="caiD"
                     /locus_tag="JN749_000487"
     CDS             40938..41723
                     /gene="caiD"
                     /locus_tag="JN749_000487"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HAY7367010.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            41729..42319
                     /gene="caiE"
                     /locus_tag="JN749_000488"
     CDS             41729..42319
                     /gene="caiE"
                     /locus_tag="JN749_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HAY7367011.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="JN749_000489"
     CDS             complement(42405..42800)
                     /gene="caiF"
                     /locus_tag="JN749_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HAY7367012.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(43063..46284)
                     /gene="carB"
                     /locus_tag="JN749_000490"
     CDS             complement(43063..46284)
                     /gene="carB"
                     /locus_tag="JN749_000490"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAY7367013.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(46302..47450)
                     /gene="carA"
                     /locus_tag="JN749_000491"
     CDS             complement(46302..47450)
                     /gene="carA"
                     /locus_tag="JN749_000491"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAY7367014.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(47905..48726)
                     /gene="dapB"
                     /locus_tag="JN749_000492"
     CDS             complement(47905..48726)
                     /gene="dapB"
                     /locus_tag="JN749_000492"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAY7367015.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(48893..49807)
                     /gene="rihC"
                     /locus_tag="JN749_000493"
     CDS             complement(48893..49807)
                     /gene="rihC"
                     /locus_tag="JN749_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HAY7367016.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(49873..50823)
                     /gene="ispH"
                     /locus_tag="JN749_000494"
                     /gene_synonym="lytB"
     CDS             complement(49873..50823)
                     /gene="ispH"
                     /locus_tag="JN749_000494"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HAY7367017.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(50825..51274)
                     /gene="fkpB"
                     /locus_tag="JN749_000495"
     CDS             complement(50825..51274)
                     /gene="fkpB"
                     /locus_tag="JN749_000495"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HAY7367018.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(51361..51855)
                     /gene="lspA"
                     /locus_tag="JN749_000496"
     CDS             complement(51361..51855)
                     /gene="lspA"
                     /locus_tag="JN749_000496"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HAY7367019.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(51855..54671)
                     /gene="ileS"
                     /locus_tag="JN749_000497"
     CDS             complement(51855..54671)
                     /gene="ileS"
                     /locus_tag="JN749_000497"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAY7367020.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(54714..55655)
                     /gene="ribF"
                     /locus_tag="JN749_000498"
     CDS             complement(54714..55655)
                     /gene="ribF"
                     /locus_tag="JN749_000498"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HAY7367021.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(55663..55881)
                     /locus_tag="JN749_000499"
     CDS             complement(55663..55881)
                     /locus_tag="JN749_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HAY7367022.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            55984..56247
                     /gene="rpsT"
                     /locus_tag="JN749_000500"
     CDS             55984..56247
                     /gene="rpsT"
                     /locus_tag="JN749_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAY7367023.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        13545 a        15496 c        14681 g        12852 t
ORIGIN      
        1 tgcccccgct cttccaggca ttaaatcctg aaaccatagc atcccaatac ccgcaacaat
       61 gccgataaat acagcgttaa aaagttgcag cgtcatcaac gccatacggc tatgaaagaa
      121 aatcaatcca gtgtaaaaca gcactcctgc caccactgca atgaccatca ttcgccgctt
      181 accataacgt ttgacatagt agccagccag aatcattgct ggtatttcca gtccagctgc
      241 cgtccccatc aggaaacccg ccagtttgtc tggcaatcct aaatcgctac tgatccacaa
      301 cggcatatca ataatgtaca tagtgttgca ggtccacatt aacgtcgagg cgacaaataa
      361 catccgtacg ttactatcct gccagtcacc ttgcattgat aaagcatttt ccgacggcag
      421 ttctacccgc gccacagacg gaagcataaa tgcaatcaat accagactga gtgtgaatat
      481 cccggcggca atcgaaaaca tcactgtaaa gccgtaattc aacgccagca taaaggccag
      541 cggtggaccg attacccatg ccagagaaag ctgcgcacgc atcaccgagc taaacatcac
      601 cacttctcgc gccgagttat ccgcgtattc ccgcgccaga gcaaataact gtggcattgc
      661 cgtattggcc agagatgcca gaagcacacc acaggtgata agcgtcagat aatgacgatt
      721 aaatgcaaac agtagcgcat tgccaatagc catcaaacag caaaatataa tcagttttcg
      781 ccgatcgccc tgactgtcag aacgttttgc caaccagagg cttacgccga tcccagcaat
      841 agcattcacc gtataaaaga gtccgatcca gaaaggttgc gcgccaacct cacgactcag
      901 aaataagctc aatgtaggag cctgtagcgc cccggccacc cccatcataa aagcgaccag
      961 cataaatgcc gcgtaaacac cgttcatacg gcgagccatc gtcattatcc agatcatacg
     1021 ttcccttttt agcgcggcga gaataaaaaa aaccagcagg tataatctgc tggcgggtga
     1081 ttttacacca atactcagtc acacatgatg caggcaagtc aactttcaga attgcggtca
     1141 tcccactgca tcagtccttc aagcatggtt aatcgttgtg ggacgcttaa ccaacgcaac
     1201 cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa actgacgcat ggggcatctc
     1261 cttgcttcat cgttaggaat tattgactta atatagggaa aataaaattg ctgtcttttg
     1321 cgcaggagtt ccccttttat gccatctgct cgtctgcaac aacagttcat ccgcctgtgg
     1381 caatgctgcg agggtaaatc gcaggacaca acgctcaacg aactggcagc gttattgagc
     1441 tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc aggatcgcgg ctggctgacg
     1501 tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga cattcctcta taccgggctg
     1561 gcgcttcagc aacagcgggc ggaagacctg ctggagcagg atcgtatcga ccaactagtg
     1621 cagttggttg gcgacaaagc gactgtgcgg caaatgctgg tttctcatct gggccgcagc
     1681 ttccgccagg ggcggcacat cctgcgcgtg ctctactatc gtccgttgcg taatctgcta
     1741 cctggcagcg cattgcgccg ttccgaaacc catattgccc ggcaaatctt cagttcgcta
     1801 acgcgcataa atgaggaaaa tggggaactg gaagcagaca tcgcccacca ctggcagcaa
     1861 atatcaccgc ttcactggcg tttctttttg cgtccaggag tccattttca tcatggtcgt
     1921 gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa tcaatacgct gccgctctat
     1981 tcgcatatta cggacattgt gtcgccgacg ccctggacgc tggatatcca tctcacgcag
     2041 ccggaccgct ggttgccgtt actgctgggg caagttccgg cgatgatcct gccgtgcgaa
     2101 tgggaaaccc tcagtaactt tgccagccat cctattggca ccggtccgta tgcggtgatt
     2161 cgcaacagca ccaatcaact gaaaattcag gcattcgatg acttcttcgg ttaccgggca
     2221 ttaatcgacg aagttaacgt ctgggttctg ccggaaattg ccgacgagcc agccggtgat
     2281 ctgatgctaa aaggtccaca gggcgaggaa aaagagattg aaagccgcct ggaggaaggt
     2341 tgctactatt tactgttcga cagccgcacc catcgcgggg cgaatcagca agtcagggac
     2401 tgggtaagct atgtgctttc tccaactaat ctggtctatt tcgctgagga acagtaccag
     2461 caactgtggt tcccggctta tggactgctc ccccgttggc accatgcccg caccataaag
     2521 agcgaaaaac cggctggcct ggaaagcctc accctgacct tttatcagga tcacagtgag
     2581 catcgggtga ttgccgggat catgcagcag attctggcaa gtcaccaggt cacgctggaa
     2641 atcaaagaga tcagctacga tcagtggcat gaaggagaga tcgagagcga tatctggctt
     2701 aatagcgcca actttacgct gccgctggat ttttcgctgt tcgcgcactt gtgcgaggtg
     2761 ccgctgctcc aacactgtat tccgatcgac tggcaagccg acgccgcccg ctggcgcaat
     2821 ggcgaaatga acctggcgaa ctggtgccag caactggtcg ccagtaaagc aatggtgccg
     2881 cttatccacc actggctgat cattcaggga caacgcagta tgcgcggcct gcgtatgaat
     2941 accctcggct ggttcgattt taaatcagcg tggtttgcgc caccggatcc atgattgctg
     3001 gtagataaca aaatcactac actaacgccg ttctcaacgg ggtgccacgc aaacgcgtgc
     3061 gctgagaaaa tacccgtcga acctgatccg gataacgccg gcgaagggat ttgaggctcc
     3121 ttctcaagtc ctttgccact cttttttgag gtgcaaagtg ttaaaaaaat gtcttcccct
     3181 gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt ctgactgttt atacctacga
     3241 ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa aaagcttttg aagccgactg
     3301 taactgcgaa ctgaaactgg tggcgctgga agatggcgtt tcgcttctca accgtctacg
     3361 gatggaaggc aaaaatagta aagccgatgt ggtgctgggg ctggataaca atctgttaga
     3421 cgccgccagt aaaaccggac tatttgccaa aagcggtgtg gcagcggatg ccgttaacgt
     3481 tcccagcggc tggaataatg acactttcgt cccgtttgat tacggctatt tcgccttcgt
     3541 ctatgacaag aacaaactga aaaacccgcc gcaaagcctg aaagaattgg ttgagagcga
     3601 tcagaactgg cgggtgattt atcaggatcc gcgtaccagc acgccggggc tgggtctgct
     3661 gttgtggatg caaaaagtct atggcgatga cgccccacaa gcctggcaaa aactggcgaa
     3721 gaaaaccgtc acggtcacca aaggctggag cgaagcctac ggcctgtttt taaaaggtga
     3781 aagcgatctg gtactgagtt acaccacctc tccggcttat cacattctcg aagagaaaaa
     3841 agataactac gccgccgcga actttagcga aggtcactat ctgcaggtgg aagtcgccgc
     3901 ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa ttcctccagt ttatggtttc
     3961 tccggctttt cagaatgcga tcccaaccgg taactggatg tatccggtgg caaatgtcac
     4021 gctgcctgcc gggtttgaac aattgaccaa accggccacc acgctggagt tcacaccagc
     4081 tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg caacgcgccg tcagccgtta
     4141 attcccggct ggttaattcc aggtgtaagc gccgccacgc tggtggtagc ggttgcgctc
     4201 gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta actgggtggc agtctggcag
     4261 gacagctacc tgtggcatgt ggtgcgcttc tccttctggc aggcgtttct ctcggcgcta
     4321 ctctctgtcg tacccgcgat attcctcgcc cgcgccctct atcgcaggcg ctttccgggt
     4381 cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc tcccggtgct agttgctgtt
     4441 ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg catcgctctg ccaatcgctc
     4501 ggtctggagt ggaccttttc gccctacggc ctgcaaggca ttttgctggc gcacgtattt
     4561 tttaatctgc cgatggcgag ccgcttatta ctccaggcac tggaaaacat tcccggcgaa
     4621 caacgtcagc ttgccgccca gcttgggatg cgcggctggc attttttccg cttcgtcgaa
     4681 tggccgtggc tacggcgaca aatcccgccg gttgccgcgc ttatctttat gctctgtttc
     4741 gccagcttcg ccaccgtgct atcgctgggc ggcggtccgc aggcaaccac tatcgagctg
     4801 gctatttatc aggcgctgag ttacgactac gatcctgccc gcgcggcgat gctggcgctg
     4861 ctccagatgg tgtgttgcct tgggctggtg ctgttgagtc agcgactaag taaggccatt
     4921 gcgcccggca ccacgctgct gcaaggctgg cgcgatccgg acgatcgtct gcatagccgc
     4981 atttgcgaca cggtgttaat agtgctggcg ctgctgctgt tgctgccacc gttgctggcg
     5041 gtgatcgtcg atggggtaaa tcgccagttg ccggaagtgc tggcacaacc ggtgctgtgg
     5101 caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag gtgtattgtg cgtagtgctg
     5161 accatgatgc tgctatggag cagtcgcgaa ctgcgggcgc ggcagaaaat gctggcgggt
     5221 caggcgctgg agatgagcgg catgttgatc ctcgccatgc cggggattgt gctggctacc
     5281 ggcttctttt tactgctcaa caacactatc ggcctgccac aatctgctga cggcattgtg
     5341 attttcacca atgcgttaat ggcgatccct tatgcgctga aagtactgga aaacccgatg
     5401 cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc tgggcattga aggctggtcg
     5461 cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac tggcgcaggc gctggctttt
     5521 gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt tgttcggtaa cgatgatttc
     5581 cgtaccctgc cgttttatct ctaccagcaa attggctcct atcgcagcca ggacggcgcg
     5641 gtcaccgcgt taattctgct actactctgt tttctgctgt ttaccgtgat tgaaaaacta
     5701 ccggggcgaa atgttaaaac tgactgatat cacctggctt tatcaccatt tgccgatgcg
     5761 ttttagctta acggtagaac gcggcgagca ggtggcaatc ctcgggccaa gcggcgcggg
     5821 taaaagtacc ctgctgaatt tgattgccgg ttttctgacg ccagccagcg gttcgctgac
     5881 tatcgatggc gttgatcaca ccacgacgcc gccgtcacgc cgtccggtgt cgatgctgtt
     5941 tcaggagaac aacctgttca gccacctgac ggtcgcgcag aacatcgggc tggggctaaa
     6001 tccggggttg aaactgaacg cggcacagca ggagaaaatg cacgcgattg cccgtcagat
     6061 ggggattgat aatttaatgg cgcggttacc gggcgagctt tccggcggtc agcgacagcg
     6121 cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt ttattgctcg atgaaccgtt
     6181 ctctgcgctc gatccggcgt tacgtcagga gatgctgacg ctggtgagca cgagctgcca
     6241 gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg gaagatgcgg cgcggatcgc
     6301 cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg cagggtaaga ctgaagaatt
     6361 gttgagcggt aaggcgagtg cttcggcaat attggggatt acgggttagt atgcactgtc
     6421 agatgcggcg tgaacacctt atccgaccta cggacagtgc gttttgtagg cctgataaga
     6481 cgcggtcagc gtcgcatgag gcaacccgca caagacaatc ccctaaaccc caaccacttt
     6541 acgcaaaata tcgatataca ccggcatcaa cgggtggcga attaacacca ccagcgccac
     6601 cacgccgatg gcagaaatca acggcgtcag ccacaacaaa cggccgcgcg acaaataatg
     6661 acttaaacga tcggttgctt taccgctgcg ccataaccgc cagcacagcc agccaccaac
     6721 ccacaaaaat accgccgtcg ccagcagcaa ccatttaaac tcgccgctct gcattccggc
     6781 tggaatatcg atcgccgcgc ccgccagaat cccaggcagg aagtaaaacg gtggccacag
     6841 caggcagccg ataatattcg gcgtaatgaa ttttgccacc ggcagatcca gcattcccgc
     6901 caccattggc accagcggac gcgtcgggcc aacaaagcga ccgaccagaa tggtgaacat
     6961 gctgtgttga tgcaacgcat gttcagtttt atcgagtaat gctttgtttt tcttcagaaa
     7021 tgaccagcga tgcaacggct ttttaaaacg ccagcccagc cagaaggaga tccagtcgcc
     7081 cagcagacaa ccgacaatcc ctgccagcca agcgtgccag aaacttaact cgccgctgcc
     7141 aatcagcgct cccagccccg ccatcagcac cgtaccgggt agaatcaaac cgaccagcgc
     7201 cagcgactcc agaaaggcca ccaacaccac cgccatcaat gaatacacgg tagattgggt
     7261 aataaagtgt tccagcaatg cttgcataat gtgcctgtca aatggacgaa gcagggattc
     7321 tgcaaaccct atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca
     7381 acttgacggc tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg
     7441 ccccggtgca ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc
     7501 gaccgacggt ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac
     7561 gttggtcctc gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca
     7621 gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg
     7681 ccaggtgatc gctgatgtac tgacaagcct cgcgtacacg attatccatc ggtggatgga
     7741 gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca
     7801 gcagctccga atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc
     7861 tgaaatacgg ctggtgcgct tcatccgggc gaaagaaacc cgtattggca aatattgacg
     7921 gccagttaag ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc
     7981 attcgcgagc ctccggatga caaccgtagt gatgaatctc tcctggcggg aacagcaaaa
     8041 tatcacccgg tcggcagaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa
     8101 tggtgagatt gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat
     8161 aaccgttggc ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg
     8221 gcagcagggg atcattttgc gcttcagcca tacttttcat actcccgcca ttcagagaag
     8281 aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct
     8341 cgctaaccca accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag
     8401 ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt
     8461 atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc
     8521 ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttggatgg
     8581 agtgaaacga tggcgattgc aattggcctc gattttggca gtgattctgt gcgagctttg
     8641 gcggtggact gcgccagcgg tgaagagatc gccaccagcg tagagtggta tccccgttgg
     8701 caaaaagggc aattttgtga tgccccgaat aaccagttcc gtcatcatcc gcgtgactac
     8761 attgagtcaa tggaagcggc actgaaaacc gtgcttgcag agcttagcgt cgaacagcgc
     8821 gcagctgtgg tcgggattgg cgttgacagt accggctcga cgcccgcacc gattgatgcc
     8881 gacggtaacg tgctggcgct gcgcccggag tttgccgaaa acccgaacgc gatgttcgta
     8941 ttgtggaaag accacactgc ggttgaagaa gcggaagaga ttacccgttt gtgccacgcg
     9001 ccgggcaacg ttgactactc ccgctatatt ggcggtattt attccagcga atggttctgg
     9061 gcaaaaatcc tgcatgtgac tcgccaggac agcgccgtgg cgcaatctgc cgcatcgtgg
     9121 attgagctgt gcgactgggt gccagctctg ctttccggta ccacccgccc gcaggatatt
     9181 cgtcgcggac gttgcagcgc cgggcataaa tctctgtggc acgaaagctg gggcggcttg
     9241 ccgccagcca gtttctttga tgagctggac ccgatcctca atcgccattt gccttccccg
     9301 ctgttcactg acacctggac tgccgatatt ccggtgggca ccttatgccc ggaatgggcg
     9361 cagttggcgc aggcgcacag cctaacgcac tggtaaaagt tatcggtact tccacctgcg
     9421 acattctgat tgccgacaaa cagagcgttg gcgagcgggc agttaaaggt atttgcggtc
     9481 aggttgatgg cagcgtggtg cctggattta tcggtctgga agcaggccaa tcggcgtttg
     9541 gtgatatcta cgcctggttt ggtcgcgtac tcggctggcc gctggaacag cttgccgccc
     9601 agcatgcgga actgaaagcg caaatcaacg ccagccagaa acaactgctt ccggcgctga
     9661 ccgaagcatg ggccaaaaat ccgtctctgg atcacctgcc ggtggtgctc gactggttta
     9721 acggtcgtcg cacgccaaac gctaaccaac gcctgaaagg ggtgattacc gatcttaacc
     9781 tcgctaccga cactccgctg ctgttcggcg gtttgattgc tgccaccgcc tttggcgcac
     9841 gcgcaatcat ggagtgcttt accgatcagg ggatcgccgt caataacgtg atggcgctgg
     9901 gcggcatcgc gcggaaaaac caggtcatta tgcaggcctg ctgcgacgtg ctgaatcgcc
     9961 cgctgcaaat tgttgcctct gaccagtgct gtgcgctcgg tgcggcgatt tttgctgccg
    10021 tcgccgcgaa agtgcacgca gacatcccat cagcccagca aaaaatggcc agtgcggtag
    10081 agaaaaccct gcaaccgcgc agcgaacagg cacaacgctt tgaacagctt tatcgccgct
    10141 atcagcaatg ggcgatgagc gccgaacaac actatcttcc aacttccgcc ccggcacagg
    10201 ctgcccaggc cgttgcgact ctataaggac acgataatga cgatttttga taattatgaa
    10261 gtgtggtttg tcattggcag ccagcatctg tatggcccgg aaaccctgcg tcaggtcacc
    10321 caacatgccg agcacgttgt taatgcgctg aatacggaag cgaaactgcc ctgcaaactg
    10381 gtgttgaaac cgctgggcac cacgccggat gaaatcaccg ctatttgccg cgacgcgaat
    10441 tacgacgatc gttgcgctgg tctggtggtg tggctgcaca ccttctcccc ggccaaaatg
    10501 tggatcaacg gcctgaccat gctcaacaaa ccgttgctgc aattccacac ccagttcaac
    10561 gcggcgctgc cgtgggacag tatcgatatg gactttatga acctgaacca gactgcacat
    10621 ggcggtcgcg agttcggctt cattggcgcg cgtatgcgtc agcaacatgc cgtcgttacc
    10681 ggtcactggc aggataaaca agcccatgag cgtatcggct cctggatgcg tcaggcggtc
    10741 tctaaacagg atacccgtca tctgaaagtc tgccgatttg gcgataacat gcgtgaagtg
    10801 gcggtcaccg atggcgataa agttgccgca cagatcaagt tcggtttctc cgtcaatacc
    10861 tgggcggttg gcgatctggt gcaggtggtg aactccatca gcgacggcga tgttaacgcg
    10921 ctggtcgatg agtacgaaag ctgctacacc atgacgcctg ccacacaaat ccacggcgaa
    10981 aaacgacaga acgtgctgga agcggcgcgt attgagctgg ggatgaagcg tttcctggaa
    11041 caaggtggct tccacgcgtt caccaccacc tttgaagatt tgcacggtct gaaacagctt
    11101 ccaggtctgc ccgtacagcg tctgatgcag cagggttacg gctttgcggg cgaaggcgac
    11161 tggaaaaccg ccgccctgct tcgcatcatg aaggtgatgt caaccggtct gcagggcggc
    11221 acctccttta tggaggacta cacctatcac ttcgagaaag gtaatgacct ggtgctcggc
    11281 tcccatatgc tggaagtctg cccgtcgatt gccgtagaag agaaaccgat cctcgacgtt
    11341 cagcatctcg gtattggtgg taaggacgat cctgcccgac tgatcttcaa tacccaaacc
    11401 ggtccagcga ttgtcgccag cctgattgat ctcggcgatc gttaccgtct gctggttaac
    11461 tgtatcgaca cggtgaaaac accgcactcc ctgccgaaac tgccggtggc taatgcactg
    11521 tggaaagcgc aaccggatct gccaactgct tccgaagcgt ggatcctcgc tggtggcgcg
    11581 caccataccg tcttcagcca tgcgctgaac ctcaacgata tgcgccagtt cgccgagatg
    11641 cacgacattg aaatcacggt gattgataac gacacccgcc tgccagcgtt taaagacgca
    11701 ctgcgctgga acgaagtgta ttacggattt cgtcgctaag tagccacatc aggtatgtaa
    11761 cgcctgatgc gacgctgact cgtcttatct ggcctacacg ctgcgatttt gtaggccgga
    11821 taagcaaagc gcatccggca ttcaacgcct gatgcgacgc tggcgcgtct tatcaggcct
    11881 acgcgctgcg attttgtaga ccggataagc gaagcgcatc cggcacgaag gagtcaacat
    11941 gttagaagat ctcaaacgcc aggtattaga agccaacctg gcgctgccaa aacacaacct
    12001 ggtcacgctc acatggggca acgtcagcgc cgttgatcgc gagcgcggcg tctttgtgat
    12061 caaaccttcc ggcgtcgatt acagcgtcat gaccgctgac gatatggtcg tggttagcat
    12121 cgaaaccggt gaagtggttg aaggtacgaa aaagccctcc tccgacacgc caactcaccg
    12181 gctgctctat caggcattcc cctccattgg cggcattgtg catacgcact cgcgccacgc
    12241 caccatctgg gcgcaggcgg gtcagtcgat tccagcaacc ggcaccaccc acgccgacta
    12301 tttctacggc accattccct gcacccgcaa aatgaccgac gcagaaatca acggcgaata
    12361 tgagtgggaa accggtaacg tcatcgtaga aacctttgaa aaacagggta tcgatgcagc
    12421 gcaaatgccc ggcgttctgg tccattccca cggcccgttt gcatggggca aaaatgccga
    12481 agatgcggtg cataacgcca tcgtgctgga agaggtcgct tatatgggga tattctgccg
    12541 tcagttagcg ccgcagttac cggatatgca gcaaacgctg ctggataaac actatctgcg
    12601 taagcatggc gcgaaggcat attacgggca gtaatgactg tataaaacca cagccaatca
    12661 aacgaaacca ggctatactc aagcctggtt ttttgatgga ttttcagcgt ggcgcaggca
    12721 ggttttatct taacccgaca ctggcgggac accccgcaag ggacagaagt ctccttctgg
    12781 ctggcgacgg acaacgggcc gttgcaggtt acgcttgcac cgcaagagtc cgtggcgttt
    12841 attcacgccg atcaggttcc ccgcgctcag catattttgc agggtgaaca aggctttcgt
    12901 ctgacaccgc tggcgttaaa ggattttcac cgccagccgg tgtatggcct ttactgtcgc
    12961 gcccatcgcc aattgatgaa ttacgaaaag cgcctgcgtg aaggtggcgt taccgtctac
    13021 gaggccgatg tgcgcccgcc agaacgctat ctgatggagc ggtttatcac ctcaccggtg
    13081 tgggtcgagg gtgatatgca caatggcact atcgttaatg cccgtctgaa accgcatccc
    13141 gactatcgtc cgccgctcaa gtgggtttct atagatattg aaaccacccg ccacggtgag
    13201 ctgtactgca tcggcctgga aggctgcggg cagcgcatcg tttatatgct ggggccggag
    13261 aatggcgacg cctcctcgct tgatttcgaa ctggaatacg tcgccagccg cccgctgctg
    13321 ctggaaaaac tcaacgcctg gtttgcaaac cacaatcccg atgtgatcat cggctggaac
    13381 gtggtgcagt tcgatctgcg aatgctgcaa aaacatgccg agcgttaccg tcttccgctg
    13441 cgtcttgggc gcgataatag cgagctggag tggcgcgagc acggctttaa aaacggcgtc
    13501 ttttttgccc aggctaaagg tcggctaatt atcgacggta tcgaggcgct gaaatccgcg
    13561 ttctggaatt tctcttcatt ctcgctggaa actgtcgctc aggagctatt aggcgaagga
    13621 aaatctatcg ataacccgtg ggttcgaatg gacgaaattg accgccgttt cgccgaagat
    13681 aaacctgcgc tggcaactta taacctgaaa gattgcgagc tggtgacgca gatcttccac
    13741 aaaactgaaa tcatgccatt tttactcgaa cgagcaacgg tgaacggcct gccggtggat
    13801 cggcacggcg gttcggtggc agcgtttggt catctctatt ttccgcgaat gcatcgcgct
    13861 ggttatgtcg cgcctaatct cggcgaagtg ccgccgcacg ccagccctgg cggctacgtg
    13921 atggattcac ggccagggct ttatgattca gtgctggtgc tggactataa aagcctgtac
    13981 ccgtcgatca tccgcacctt tctgattgat cccgtcgggc tggttgaagg catggcgcaa
    14041 cctgatccag agcacagtac cgaaggtttt ctcgatgcct ggttctcacg ggagaaacat
    14101 tgcctgccgg agattgtgac taacatctgg cacgggcgcg atgaagccaa acgtcagggt
    14161 aataagccgc tttcgcaggc gctgaagatc attatgaatg ccttttatgg cgtgctcggc
    14221 accaccgcct gccgcttctt cgatccgcgc ctggcatcgt cgatcaccat gcgtggtcat
    14281 cagatcatgc ggcagaccaa agcattgatt gaagcgcagg gctacgacgt tatctacgga
    14341 gataccgact cgacttttgt ctggctaaaa ggtgcacatt cggaagaaga ggcggcgaaa
    14401 atcggtcgtg cactggtgca gcacgttaac gcctggtggg cggaaacgct gcaaaaacaa
    14461 cggctgacca gcgcattaga actggagtat gaaactcatt tctgccgttt tcttatgcca
    14521 accattcgcg gagctgatac cggcagtaaa aagcgctacg ccgggctgat tcaggagggc
    14581 gacaagcagc ggatggtgtt taaagggctg gaaaccgtgc gcaccgactg gacgccgctg
    14641 gcccagcagt ttcagcagga gctgtacctg cgtatcttcc gcaacgagcc atatcaggag
    14701 tacgtccgcc aaaccatcga caaactgatg gcgggtgaac tggatgcgcg gctggtttac
    14761 cgtaaacgcc ttcgccgtcc gctgagcgaa tatcagcgta atgttccgcc tcatgtacgc
    14821 gccgctcgcc ttgccgatga agaaaatcaa aagcgcggtc gccccttgca atatcagaat
    14881 cgcggcacca ttaagtacgt atggaccacc aacggcccgg agccgctgga ctaccaacgt
    14941 tcaccactgg attacgaaca ctatctgacc cgccagctac aacccgtggc ggagggaata
    15001 ctccctttta ttgaggataa ttttgctaca cttatgaccg ggcaacttgg gctattttga
    15061 gcaaaaaaag agttcgccag ataccatttt gatgcgtgac gaatgctttg ccatccagta
    15121 ccatagcgcc ctttccattc ctggacctga ataacaccac tacctcataa gcacggtagc
    15181 gggtggttat tgcctgcaat taaagatata gagccgaaca catatgcctt ttacacttgg
    15241 tcaacgctgg atcagcgata cagaaagcga attgggactt ggaaccgttg tcgcggtgga
    15301 tgcgcgaact gtcactttac ttttcccatc tactggtgaa aaccgtctgt acgcacgcag
    15361 tgattccccc gtgacccgcg tgatgttcaa ccctggtgat accattacca gccatgacgg
    15421 ctggcagatg caagtcgaag aagtaaaaga agaaaatggc ttgctgacct atatcggtac
    15481 tcgcctggat actgaagagt ccggcgtagc cctgcgtgaa gttttccttg atagcaaact
    15541 ggtgttcagc aaaccgcagg accgtctgtt tgccgggcag attgaccgta tggaccgctt
    15601 tgcgctgcgt tatcgcgcgc gtaagtattc cagcgaacag ttccgtatgc cgtacagcgg
    15661 cctgcgcggt cagcgtacca gcctgatccc gcatcagctc aacatcgctc atgatgttgg
    15721 tcgccgccac gcgccgcgcg tcctgctggc tgacgaagtg ggtttaggga aaaccattga
    15781 agccgggatg atcctgcatc agcaactgct ctctggcgct gctgaacgtg tgctgattat
    15841 cgtcccggaa accttacagc atcagtggct ggtagaaatg ctgcgccgtt tcaacctgcg
    15901 ctttgctctg tttgatgatg agcgttatgc cgaagctcag cacgatgctt acaacccgtt
    15961 tgacaccgaa cagctggtga tttgctcgct ggattttgcc cgtcgtagca aacagcgcct
    16021 ggaacatctc tgtgaagccg aatgggacct gctggtggtc gatgaagcgc atcacctggt
    16081 gtggagcgaa gatgcgccaa gccgtgaata tcaggccatt gaacaactgg cagagcacgt
    16141 gccgggcgtt ctgctgctga ccgcgacccc ggaacagctg gggatggaaa gccacttcgc
    16201 ccgtctgcgt ctgctggacc cgaaccgttt ccacgatttt gcgcagttcg ttgaagagca
    16261 gaaaaattat cgtccggttg cggacgccgt tgccattctg ctggcaggta acaaactgag
    16321 caatgacgaa ctgaacatgc tcggcgagat gatcggcgag caggatatcg agccgctgtt
    16381 gcaggcagca aacagcgaca gcgaagatgc ccagagcgcc cgtcaggagc tggtttcgat
    16441 gctgatggat cgccacggca ccagccgcgt gctgttccgt aacacgcgta acggtgtgaa
    16501 aggattcccg aaacgcgagc tgcacaccat taagctgccg ctaccgacgc agtatcagac
    16561 ggctattaaa gtctccggca ttatgggcgc acgtaaaagt gcggaagatc gtgctcgcga
    16621 tatgctctac ccggagcgta tttatcagga atttgaaggt gataacgcca cctggtggaa
    16681 cttcgatccg cgcgttgagt ggctgatggg ctacctgacc agccatcgct ctcagaaagt
    16741 gctggtgatc tgcgccaaag ctgccactgc gctgcaactg gagcaggtac tgcgcgaacg
    16801 tgaaggtatt cgcgctgcgg tgttccacga aggtatgtcg attatcgaac gtgaccgcgc
    16861 tgccgcctgg tttgccgaag aagacaccgg cgcacaggta ctgctgtgct cagaaatcgg
    16921 ttctgaagga cgtaactttc agttcgccag ccacatggtg atgtttgacc tgccattcaa
    16981 cccggatcta ctggagcagc gtattggtcg tctggatcgt atcggccagg cgcacgatat
    17041 tcagatccat gtgccttatc tggagaaaac cgctcagtcg gtgctggtgc gctggtatca
    17101 cgaaggtctg gatgcatttg agcacacctg cccgaccgga cgcactattt acgatagcgt
    17161 atacaacgat ctgattaact atctggcttc accggatcaa accgaaggct ttgacgatct
    17221 gatcaaaagc tgccgcgagc aacatgaagc gctgaaagca cagctggaac agggtcgtga
    17281 ccgcctgctg gaaatccact ccaacggtgg cgaaaaagcc caggcactgg cagaaagcat
    17341 tgaagagcag gatgacgata ccaacctgat cgccttcgcc atgaacctgt tcgatattat
    17401 cggtatcaat caggacgatc gcggcgacaa catgatcgtg ctgacgccgt ccgatcatat
    17461 gctggtgccg gacttccctg gcctgtcgga agatggcatc accatcacct ttgatcgtga
    17521 agtggcgctg gcgcgtgaag atgcacagtt tattacctgg gagcatccgc tgatccgcaa
    17581 cggtctggat ctgatccttt ctggcgatac cggtagcagc acgatttcac tgttaaaaaa
    17641 caaagcgttg ccggtaggta cgctgttggt ggaactgatt tatgtggttg aagcccaggc
    17701 tccgaagcag ttgcagctca accgcttcct gccaccgacg ccggtacgta tgctgctgga
    17761 taaaaacggc aacaacctgg cggcgcaggt agagtttgaa acctttaacc gccagcttaa
    17821 cgcggttaac cgtcacaccg gcagcaaact ggttaacacc gtgcagcagg atgttcacgc
    17881 tatccttcaa ctgggtgaag cgcagatcga gaaatctgcc cgtgcattga ttgatgcagc
    17941 gcgtaacgaa gccgacgaaa aactgtctgc cgagctgtct cgtctggaag ctctgcgtgc
    18001 agtgaacccg aacattcgtg acgacgaact gaccgccatt gagagcaacc gtcagcaggt
    18061 aatggaaagc ctggatcagg caggttggcg tctggatgcc ctgcgtttga tcgttgtaac
    18121 gcatcagtaa cggagccgaa aatggggatg gaaaactaca atccaccgca ggaaccctgg
    18181 ttggttatcc tgtatcagga tgaccatatt atggtggtca acaagccgag cggtttgttg
    18241 tcagtgccgg gtcgtctgga agagcacaaa gacagcgtga tgacgcgcat tcagcgtgat
    18301 tatccgcagg cagaatcggt gcatcgtctg gatatggcta ccagcggcgt gattgtagtg
    18361 gcgctgacca aagccgcgga gcgggagtta aaacgccagt tccgcgagcg cgagccgaaa
    18421 aagcagtatg tggcccgcgt ctgggggcat ccatcccccg cagaaggtct ggtggatctg
    18481 ccgctgattt gcgactggcc aaaccgcccg aaacagaaag tctgttacga aacgggtaaa
    18541 cctgcgcaga cggaatatga agtggtggag tatgcggcgg ataacacggc aagagcggtg
    18601 ttaaaaccga ttaccgggcg ttcgcatcaa ttgcgtgtgc atatgctggc gctggggcat
    18661 ccgattctcg gcgatcgttt ttatgcatca ccagaagcga gagcgatggc accgcgtttg
    18721 ttgctacatg cagagatgtt gacgattacc catccggcgt atggcaatag tatgacgttt
    18781 aaagcgccag cggattttta atcgtcacgc tggcgcagtt cggtaggcgg ataaggcgtt
    18841 cacgccgcat ccggcaattg tgcgccgatt gcctgatgcg acgcgggccg cgtcttatca
    18901 ggcctacagt ttaaaactta tttaaaccct ttctgctgct ttatcagctc atatgcctgc
    18961 tgaatttcct gcgctttctg cttcgccatc tccatcatct caggcggcaa acctttcgcc
    19021 accagcttat cgggatggtg ttcactcatc agcttacggt aggcacgttt gatggtggtc
    19081 gcatcatccg tcggcttcac gcccagcaca ttacaggcat cttccagcgt cgggccacgc
    19141 tgcgcttgct gccagttacc accgccagat tgctgctgat aaccgccgcc aaactgtgca
    19201 ccgccctgca tcatgcgcaa aaactggtca aactgggcgc gggagatccc taattcttca
    19261 gcaatgacat acagcaccgc ccgttcattc gggtgcagtg aaccatcagc aaacgccgcc
    19321 tgaatctgga tctccagaaa catacgaatt aagtcaaaac gaccaaagca gacactgcga
    19381 aactggcgca tcttttcgcg cagcgggtaa ttatctgatt ttcccacccg gaacgcgttt
    19441 tgcgccgcag tacgggaagc gccatgaagg ttcattcggt ccatcaactg gctggcgata
    19501 tgaatatcag cctccgtgac gcgacctttg gatttggtta aatgccccat cacttcaaaa
    19561 gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg cgaaccacgc cattttacgg
    19621 ctacgggctt tatcaaacat atggccaatt aacaggccta acactacgcc ccaaaagccg
    19681 ccgcccatca gtaaggccac ggccacgcca atgatttttc cccaatactg catatattcc
    19741 ccaaatcgac acacggatat cagggctatc tcccacaata taaaggtgct tttaccgttt
    19801 tccggctgcg gtcaattgtg ggacatcgcc tataatttgc attatcatac ctgtcattca
    19861 atgccgtgcc taacaccact gacgctattc ggacaggatt aacactagcg tagagatgac
    19921 aagtacgtta gtctctgacc gtttgtcacg cgcaacgtta ccgatgatgg aacaataaaa
    19981 tcaacgtatg aaaaaacgta tccccactct cctggccacc atgattgcca ccgcccttta
    20041 tagtcaacag ggactggcag ccgacctcgc ctcacagtgc atgttgggcg tgccaagcta
    20101 tgaccgtcct ctggtacagg gcgataccaa tgacttaccc gtgactatca atgctgacca
    20161 cgcgaaaggg gactacccgg atgacgccgt gtttactggc agcgtggata tcatgcaggg
    20221 taacagccgt ctgcaggccg acgaagtgca gctccatcaa aaagaggcac caggacaacc
    20281 ggagccggta cgtaccgttg atgcgctcgg taatgtccat tacgacgata accaggtgat
    20341 cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa gataccaacg tctgggaagg
    20401 tgattaccag atggtgggtc gccagggtcg cggtaaagcg gacctgatga aacaacgtgg
    20461 cgaaaaccgc tataccattc tggataacgg tagctttacc tcctgtctgc cgggttctga
    20521 cacctggagc gtggtaggta gcgaaattat tcatgaccgc gaagaacaag ttgcggagat
    20581 ctggaacgcc cgctttaagg tgggtccggt accgatcttt tatagcccct atttgcagtt
    20641 gccggtgggt gacaaacgtc gctctggttt cttgatcccg aacgccaagt acaccaccac
    20701 caactacttt gagttctacc tgccatatta ctggaacatc gcgccaaata tggatgccac
    20761 catcacgccg cattatatgc atcgtcgtgg caacatcatg tgggagaacg aattccgcta
    20821 cctctcccag gcgggcgctg gcttgatgga actggactat ctgccttcag ataaagtcta
    20881 tgaagatgaa cacccgaacg atgacagttc acgtcgttgg ttattctact ggaaccactc
    20941 cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac accaaggtca gcgatcctag
    21001 ctacttcaat gatttcgata acaagtacgg ttccagtact gacggctacg caacgcaaaa
    21061 attcagcgtt ggctatgcgg tgcaaaactt caatgccacc gtttcaacca agcagttcca
    21121 ggttttcagc gaacagaaca ccagtagcta ctcggcagag ccgcagttag acgttaatta
    21181 ctaccagaat gatgttggtc cgtttgatac gcgtatttac ggccaggcag tgcactttgt
    21241 taacaccaga gacgacatgc ctgaagcaac ccgtgttcac ctggaaccga ccatcaattt
    21301 gccgctctct aataactggg gcagcatcaa taccgaagcg aagtttctgg caacccatta
    21361 tcagcaaacc aatcttgact ggtataactc cagaaacacg accaagctgg acgaatccgt
    21421 taaccgcgta atgccgcaat tcaaagttga cggcaaaatg gtctttgaac gcgatatgga
    21481 aatgctggct ccgggttata cccaaacgct ggaaccgcgc gcgcagtatt tgtacgtgcc
    21541 gtatcgcgat cagagcgaca tctataacta cgactcgtct ctgctgcaat ctgactactc
    21601 tggcctgttc cgggaccgga cttacggcgg tcttgaccgt attgcctccg ctaaccaggt
    21661 gacgaccggt gtcacatctc gcatatatga tgatgctgcc gttgaacgtt ttaatatttc
    21721 cgttggtcaa atctactatt tcacggagtc tcgcactggc gatgacaaca taacatggga
    21781 gaatgacgac aaaacgggct cactggtgtg ggcaggcgac acctactggc gtatctccga
    21841 gcgttgggga ttgcgtggcg ggattcagta cgatacacgt ctggataacg tagcgaccag
    21901 taactccagc attgaatacc gtcgggatga agaccgtctg gtacagctga attaccatta
    21961 cgccagcccg gaatatattc aggctacgct gcctaagtac tattccactg ctgagcaata
    22021 taagaatggt atttcgcagg taggtgctgt cgccagcagg ccaattgccg atcgttggtc
    22081 cattgttggg gcctactact acgacaccaa tgcgaacaag caagccgact ctatgttagg
    22141 tgtgcaatac agctcctgct gctatgcaat tcgcgtcggt tacgagcgga agctgaacgg
    22201 ttgggataac gataaacaac atgcggtata tgacaacgca atcggcttta acatcgaact
    22261 tcgcggcctg agctccaact acggtctggg tacgcaagag atgctgcgtt cgaacattct
    22321 gccgtatcaa aacactttgt gatttgttga tttaccacgt aatccgcagt gcggttaatt
    22381 gaaatggaaa aagtatgaag aactggaaaa cgctgcttct cggtatcgcc atgatcgcga
    22441 ataccagttt cgctgccccc caggtagtcg ataaagtcgc agccgtcgtc aataacggcg
    22501 tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt aaaactgaac gctgcgcagg
    22561 caaggcagca gcttcctgat gacgcgacgc tgcgccacca aatcatggaa cgtttgatca
    22621 tggatcaaat catcctgcag atggggcaga aaatgggagt gaaaatctcc gatgagcagc
    22681 tggatcaggc gattgctaac atcgcgaaac agaacaacat gacgctggat cagatgcgca
    22741 gccgtctggc ttacgatggt ctgaactaca acacttatcg taaccagatc cgcaaagaga
    22801 tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg catcaccatc ctgccgcagg
    22861 aagtcgaatc cctggcgcag caggtgggta accaaaacga cgccagcact gagctgaacc
    22921 tgagccacat cctgatcccg ctgccggaaa acccgacctc tgatcaggtg aacgaagcgg
    22981 aaagccaggc gcgcgccatt gtcgatcagg cgcgtaacgg cgctgatttc ggtaagctgg
    23041 cgattgctca ttctgccgac cagcaggcgc tgaacggcgg ccagatgggg tggggccgta
    23101 ttcaggagtt gccggggatc ttcgcccagg cattaagcac cgcgaagaaa ggcgacattg
    23161 ttggcccgat tcgttccggc gttggcttcc atattctgaa agttaacgac ctgcgcggcg
    23221 aaagcaaaaa tatctcggtg accgaagttc atgctcgcca tattctgctg aaaccgtcgc
    23281 cgatcatgac tgacgaacag gcccgcgtga aactggagca aattgctgct gatatcaaga
    23341 gtggtaaaac gacttttgct gccgcagcga aagagttctc gcaggatcca ggttctgcta
    23401 accagggcgg tgatctcggc tgggctacac cagatatttt cgatccagcc ttccgtgacg
    23461 ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt tcactcttca ttcggctggc
    23521 atttaatcga actgctggat acccgtaatg tcgataaaac cgacgctgcg cagaaagatc
    23581 gtgcataccg catgctgatg aaccgtaagt tctcggaaga agcagcaagc tggatgcagg
    23641 aacaacgtgc caacgcctac gttaaaatcc tgagcaacta atggttaaaa cccaacgtgt
    23701 tgtgatcact cccggcgagc ccgccgggat tggcccggac ttagttgtcc agcttgcaca
    23761 gcgtgagtgg ccggtcgaac tggttgtttg tgccgatgcc actctcctta ccgaccgggc
    23821 agcgatgctc ggtttgccgc tcaccctccg cccttattcc cccaactccc ctgcacaacc
    23881 gcaaactacg ggcacattaa cgctacttcc tgtcgcgcta cgtgaatctg tcactgcggg
    23941 gcagttagcg attgaaaatg ggcattacgt ggtggagacg ctggcgcgag cgtgtgatgg
    24001 ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc gtgcataaag gcgtcattaa
    24061 cgacgcaagc attccgttta ccggtcatac cgagtttttc gaagagcgtt cgcaggcgaa
    24121 aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg gcgctggcaa cgacgcattt
    24181 accgctgcgc gatatcgcag atgctatcac ccctgcactt ttgcacgaag tgattgctat
    24241 tttgcatcac gatttgcgta caaaatttgg tattgccgaa ccgcgcattc tggtctgcgg
    24301 gctgaatccg cacgcgggcg aaggcggtca tatgggtacg gaagagatag acaccattat
    24361 tccggtgctc gacgagctgc gggcgcaggg aatgaaactc aacgggccgc tgcctgccga
    24421 taccctgttt cagccgaaat atctcgataa cgccgacgcc gtgctggcga tgtaccacga
    24481 tcagggtctt cccgtgctaa aataccaggg cttcgggcgc ggtgtgaaca ttacgctggg
    24541 cctgcccttt attcgcacat cagtggacca cggcaccgcg cttgaactgg cgggacgtgg
    24601 cgaagccgat gtcggcagtt ttattacggc gcttaatctc gccatcaaaa tgattgttaa
    24661 cacccaatga ataatcgagt ccaccagggc cacttagccc gtaaacgctt cgggcaaaac
    24721 tttctcaacg atcagttcgt gatcgacagc attgtttctg ccattaaccc gcaaaagggc
    24781 caggcaatgg tcgaaatcgg ccccggtctt gcggcattga ccgagccggt cggcgaacgt
    24841 ctggaccagc tgacggtcat cgaacttgac cgcgatctgg cggcacgtct gcaaacgcat
    24901 ccattcttag gcccgaaact gacgatttat cagcaggatg cgatgacctt taactttggc
    24961 gaactggccg agaaaatggg tcagccgctg cgtgttttcg gcaacctgcc ttataacatc
    25021 tccacgccgt tgatgttcca tctgtttagc tatactgatg ccattgccga catgcacttt
    25081 atgttgcaaa aagaggtggt gaatcgtctg gttgcaggac cgaacagcaa agcgtatggt
    25141 cgattaagcg tcatggcgca atactattgc aacgtgatcc cggtactgga agttccgccg
    25201 tcagccttta caccaccacc caaagtggat tccgccgtcg tgcgcctggt ccctcatgca
    25261 acgatgcctc acccggttaa agatgttcgc gtgttgagcc gcatcaccac cgaagccttt
    25321 aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc tgtttagcgt cgaggtgtta
    25381 accggaatgg ggatcgaccc ggcgatgcga gcggaaaata tctctgtcgc gcaatattgc
    25441 cagatggcga actatctggc ggagaacgcg cctttgcagg agagttaacg atgatcaatt
    25501 cgccccgagt gtgtattcag gttcaaagcg tctacattga ggcacaatct tcacctgata
    25561 atgaacgtta cgtttttgct tataccgtaa ccatacgcaa tctggggcga gcgccagtgc
    25621 agttgttggg gcgttactgg ctgatcacca atggcaatgg ccgtgaaacc gaagtccagg
    25681 gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg cgaagagtac cagtacacca
    25741 gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg tcactacgaa atgatcgatg
    25801 aaaatggcgt ccctttcagc atcgacattc ccgtattccg actcgccgtt cccacactca
    25861 ttcattaaaa gaatatggcg acatacctta ttggcgacgt tcatggttgt tacgatgaac
    25921 tgatcgcatt gctgcataaa gtagaattta cccccgggaa agataccctc tggctgacgg
    25981 gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct gcgctatgtg aaatccttag
    26041 gcgacagcgt acgtctggtg ctgggcaatc acgatctgca tctgctggcg gtatttgccg
    26101 ggatcagccg caataaaccg aaagatcgcc tgacaccgct gctggaagcg ccggatgccg
    26161 acgagctgct taactggctg cgtcgccagc ctctgctgca aatcgacgaa gagaaaaagc
    26221 tggtgatggc ccacgcaggg atcacgccgc agtgggatct gcagaccgcc aaagagtgcg
    26281 cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc cttctttctt gacgctatgt
    26341 acggcgatat gccaaataac tggtcaccgg aattgcgggg gctgggaaga ctgcgtttta
    26401 tcaccaacgc ctttacccgt atgcgttttt gcttcccgaa cggtcaactg gatatgtaca
    26461 gcaaagaatc gccggaagaa gcccctgccc cactgaaacc gtggtttgcg attcctggcc
    26521 ctgtcgctga agaatacagc atcgcctttg gtcactgggc atcgctggaa ggcaaaggta
    26581 cgccggaagg tatttacgcg ctggataccg gctgctgctg gggtgggtca ttaacctgcc
    26641 tgcgctggga agataaacag tattttgtcc agccgtcgaa ccggcataag gatttgggcg
    26701 aagcggcggc gtcttaaaca cagcctgata caggaaggcc ggataagacg cgaccggcgt
    26761 cgcatccggc actagccgta aattctatac aaacttaccg ccgctccaga atctcaaagc
    26821 aatagctgtg agagttctgc gcatcggcgt cgtggaattc gctgaatacc gattcccagt
    26881 catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca
    26941 gatacagttt ttgcgctttt ggcaggaact gctcataaac gcgaccgccg ccaatcacca
    27001 tgatttctgg tacgtcacca cacgccgcaa tggcttcatc caccgatttc acccacgtta
    27061 cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct ggcaacggac
    27121 gaccgattga ttcccaagta tggcggccca taatcacggg tttatttaag gtgttgcgtt
    27181 taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg ccgataacgc
    27241 gatctaccgc taacgccgca atcagactga tcattgagat ttcccgataa aaaaattgtc
    27301 gccactatac gtaaagcgta aaccgtcgtc gactggtgcg aggatgatgt tgaggaaaat
    27361 tttatattct gctggcgagt ccacgctctc cccctggact cgccgcatta caatgaaaca
    27421 aaaacaaaca gttagctgta aagtgtgatt tacgtcactc tttattaaga tgagggtttc
    27481 gtttccggtt catccgccat gttgccggta tgtttacctt cttctgttcc ctgccagcca
    27541 tgtcgttgaa ttaatgacag atgttcgcgg tcctcggtga tgatctcact taacatcgcg
    27601 ctggtgcgtt tatagaccgc cgcgcgggct ttggtgtcat tctcaaccat tgccatctct
    27661 tccaccatct gaatattaaa gcggcggaac acatcggcac gttctcgcgc ttcatacggc
    27721 cccagaccta aactttccag cgccagacgc ccggttttca gcgcaccttc aaaggtttca
    27781 cgctccggct tttcaacgcc tgcctgacgc aaacgaatgt agtgatcgac atcgcgggcg
    27841 cgggcaataa tctgcaaatg cgggaaatgt tctttcacca tctccgtcag ttgcaggttg
    27901 gtttgcggat cgtcaatagc gttaatcagc acttccgctt tcgccgctcc ggcagattcc
    27961 agtaaatcca tccgcgtggc atcgccataa aacactttca taccaaattt acgcagcgtt
    28021 tcgatatggt ccggatcgtg atcgagcacc accattttca ccccgctgga gagcagtaaa
    28081 cgtccggtaa tctgtccaaa acgaccgaat ccggcgataa tcacgcgcgg ctgttcttca
    28141 tcgatctcat cggcttcacg cgcctcctcc gtagaagatt gctcaaggcg attgaggatc
    28201 accagcagaa tcggcgttgc tgccatcgac agcgccaccg ccagggtcag cgatttcgcc
    28261 cactccggct ccagcacatt cgccatctgc gccgcaccaa ataccacaaa ggcaaactca
    28321 ctgccctgcc ctaacaacac cgcaaaccag cgacgctgtt tatttggcac ctgcaacggt
    28381 cgggcaatca gccacagcat ggcgattttg atgatgagga aaccgagcag caaaatgaca
    28441 atgcgcaatg ggttttcaat cagcgtgcca aagtctatcg acatgccaac accgatgaaa
    28501 aacagcccca acagcaaacc tttaaacggt tcgatatcgc tttccaacgc atgacggtat
    28561 tccgagcttg ccagcagtac gcccgccaga aacgcgccca tcgccatcga caagccgacc
    28621 tcttccagca gcaaaccaaa gccaaacaca aggaataacg ccacggcact aaacacttcc
    28681 cgcaagccag agcgggcaac aaaacgcagc gccggacgcg taacatagcg ccccagcaat
    28741 accaccagta ccagcgcacc cgccactttt aacgccgaga gagcaaatgc gcccatcgtc
    28801 gtcgaggcac tgctcgccgc cagtagcggg atcatcgcca ccagcgggat tgccgcgata
    28861 tcctggaaca gcagcactgc aaaggcactg cggcccattt gcgtcaccat cagattgcgt
    28921 tcattcatcg cctgcatggc aatcgccgtt gatgagagcg ccagcgtcat accgatcaat
    28981 tccgcgacct gccagcgcag cccaagtaac atacagaaca gccccagcag gccaccgcaa
    29041 atcaccatct gcaatgcgcc accgccgaac actgccgcac gcagcttcca cagcctttgt
    29101 ggatcgagtt cgaggccgat aataaacagc atcagcacca ccccaatctc ggcaaagtgc
    29161 agaatagatt cggcatcggt caccagtcgc agcccccacg ggccaataat gcagccggcg
    29221 atcaggtagc caagtaccga tcccagacca agacgtaccg caatgggtac aatcagcgcc
    29281 gccgatccga ggtagatcag cgcctgaacc agcgtatgac tatccatgat gggcctcctg
    29341 ccattccagc agacgttgct tatagtggcg cgcctgccct tcgagggttt cgtcgtcaca
    29401 aataaaggtg cagtgcatgg caaacggtgg cagccagttc agcccgcagt agattgccgt
    29461 cgcctgtagc ggctgcgaca gcacatcaaa gcccggatgc gcaccaattt caaaatggct
    29521 ttccccgccg ccggtcgtca ccgcccacag caaatgtttg ccatgcagcg ccgtgccgcc
    29581 gtgaccgtaa gcccagccgt gcgacaaaac tttatcgatc caaagtttaa gaagcggagg
    29641 aatgctgtac cactgcatcg ggtgctgcca gacgatcaga tcggcgcgag agagcgcctc
    29701 ctgctcggcg gcaatatcga tattgaagtc aggatagagt tgataaagag agcgaatttc
    29761 gacgccttcc agcgtccttg cctgttcaag catccgttta ttcgcatggg aatgatgcgg
    29821 atacggatgc gcataaatga tgaggatcat agttattagc ccgcgtgttt acgcctttgt
    29881 tttcccacaa agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa
    29941 aagagagaaa tctgaacaaa taatcccatt ctctgcccgc ggatcgtcat attgctggtc
    30001 tgcgccagtg acattcctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac
    30061 agcgagatac ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaatatc
    30121 ggcagtgccc aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg
    30181 gcagaggcgc ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc
    30241 ggcccgccag agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata
    30301 tccgggatta gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac
    30361 aatggacgcc gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag
    30421 aacaggctaa tcaccacatt ccctagtgcc gcgtttttgc caacccccaa tcccaacgaa
    30481 ccaacgattt gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag
    30541 atggcggcaa caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg
    30601 gtttgctgcg gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc
    30661 tcttcgcact cttttactcg ccctttgcgt aataaccagc ggggagattc tggcagttcg
    30721 aatcgaccaa tcaaaatcaa caaacaaggg atcgccgcgc tacccagcat ccagcgccaa
    30781 ccaccttcca catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac
    30841 cacatcgcgg caataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg
    30901 atcatcgaag tggcgatggg ataatctgca ccgatgacaa tgccgatgag tacccgcatc
    30961 accaacagtt cgacggggga tgaaacacac atcgtcgcca ccgatatcac gccgatggcg
    31021 atgatatcaa tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc
    31081 gatgtgccaa cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg
    31141 tccagtttca gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa
    31201 ccatccagaa acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat
    31261 ttgagatcgt caaagtttct ggacggttgc atgggttatt cctgtttttt tgcaatgaga
    31321 gtcagtacgg cgttacgtat caaaccaaca tcagccgtaa cggaactcca caccaaaggt
    31381 gccgcgcggg tattcccact gttccagcgc cgaccccagc cccagaatgc gacaggtgcc
    31441 gcactccaga catccggcgt aatcgaagcg cacactgccg tcatcctgct tcttgtacag
    31501 acctgcgggg cacgctttca ccagcagctc cagcacctgt ttatcagcat cggccttcac
    31561 aacaatgtgc ggatgctctt catcgacatt gaatttattg acgcccagtt tgacgtccac
    31621 attgacggga gaagtcataa aacggtcact cctttcatgc catccttgat cagattgatg
    31681 aagcccactt tcttgccgtg gcggaggatt ttcttgcgca tcagttccgg cgcactgccg
    31741 tcaatggtga acaggtcacg cgccacgccc accgccagtt ccgggtagcc gctaaacatg
    31801 cgtgggttat caaggaacgc cggtagtttc tggtacatgc gcatatcgcg cagcgggctg
    31861 ctctccagat gctgacgata ttccgcgagt ttttgcttac tgaaatcgtc gcttttcatc
    31921 gctgaaagca cggtttttgc tgcagcttcc ccggcggcaa tcgccagatc cataccgcga
    31981 atggtaaaac cgaggttcat acacattccg gcggcatcac cggcaatcaa tacgccgtca
    32041 ccgaccagct ccggcagcat gttgatgcct gcttccggca ctacgtgagc ggaatattcc
    32101 accagcttgc cgcccgcgat cagcggtgca acggccggat gctgtttgaa atcttccagc
    32161 atttgcggca ccgatttttt cgcgtcatgc agatgatgca gaccacaaac cagccccagc
    32221 gacagggtat tttcattggt ataaaggaag ccgccgccca tcaggccatc ggtgggtgat
    32281 cccgcaaaca gacaagccgc gccctgatta ccctgcaact gaaaacggtc ttcgattacc
    32341 gacttcggta actcgatcag ttccttcacg ccaaccgcca catccgtcgg tttaacgcgt
    32401 tttgccatcc ccaacttttc ggcaaggatg gagttcaccc catcagcaag gagcaccgtt
    32461 ttcgcttcaa tcacatcgcc atcggcttct acaccgacga ctttgccatc gcgctgtacg
    32521 aggttatcga cgcggatccc ggtaattaac tgcgcgcccg cttcttcggc ctgctccatc
    32581 aaccaggcat caaatttact gcgcaaaacg gagtaagaac gctgggatag cgaggtttcg
    32641 tcaccgttgc agtagtccat agtcatcgct gacttttccg tcataaacgc gagtttttca
    32701 tgggtgatca ggcgttctac gggggcggaa tcggcgaaac ctggaataat gtgttccaga
    32761 ctgtgggcat agagacgccc gccagtaacg ttcttggcac ccgcggaatt gccacgctcg
    32821 ataactaaca cttgcgcccc ttcgcgggcg agcaccagtg cggcaaccga accggcaagc
    32881 cctgcaccga cgatgatggc gtcaaagata tcttcggaca taagagctcc ctgtcagcgg
    32941 cagagccgta atgcagccct gccagagtgg atcaacgcgc taaagctgcg gtcagtgccg
    33001 gaaggatctt cacggcatcg ccaacaatgc cgtaatccgc gtactggaag atcggcgcat
    33061 ttttatcttt attgatggcg aaaatggttt gcgacgcgtt agcgccaacc atgtgctgga
    33121 tctgcccgga gatccccacc gccaggtaca gttcaggttt cagcatcagg ttggagatac
    33181 cgacatagcg ttcgtgctcc atccattttt cgttttctgc caccggacga gaacaggcca
    33241 actccgcacc tatcgccttg caaagctgtt ctgccagtgc aatgttctct ttgctgccaa
    33301 tgccgcgacc gacgctgacc accagacggg ctttgtcgag atcgacgctg ttgctctggc
    33361 gcgcctgggt tgccgtgcgg gtaatcgcca cagccggagc ctgccactcc acggtgtgcg
    33421 tttcgccagt gcgtgacgcg tctggctgag ccgcatcgaa cgtgccgctg ctgatggtca
    33481 gtaccgcata cggcgtggca atgcgttctt cgccaatcgc cagaccgccg taaaccatgt
    33541 gtttcactgt tgctttaccg tcctgtacgc tgacggtgct ggcatcgtta gacaccgccg
    33601 ctttaaggcg ataacccagt tttgccgcca gtaatttgcc gcgacgggtg tttggcaaca
    33661 gcaccagacc gtcagcgccg tgctggcgaa tagtgtcagc catgacaccg gcgtaatctt
    33721 cgatcatccg atcgtccggt ttgccgttta atttccagac atgattagcg ccgagctgga
    33781 ttgcctgtgc gccgtcggca tcattgagga caaaggcatt gatttgatta gctaaagcct
    33841 gcgcaccgtt catcagttcc ggcagacgag agggggtatc gctgaatacc cagacttgag
    33901 aaaacgtgtt catagcatcc cctgtaatta aatgactttg cgaagatttt cagcaaatgc
    33961 ggcgatctgt tcttcgccgt cgccttcaat cacgatgcgc tgacgctcgc gctgtttcgg
    34021 cgcggcaacc tgttgttcag accaggcgtt cacggcgtta aaaccaatat ccgccgccga
    34081 ccatacctgg actggctttt tcgccgcgcc gagaatggct ttcatcgaag gaatttgtgg
    34141 ggagttgata tcagtggaaa cagcaacaac cgcaggcagc ggaatgctta aggtttcggt
    34201 ttcatcttcc agttcgcgct caacggtgag ggtatctgcc gtcagggaga taattttgct
    34261 gacgccgtta actgccggaa tattgaggat ttcgcccacc agcagaccaa cctgctgggc
    34321 ataaaggtcg gaagaaccat cgccacagag gatcagatca aagcctgctt tctgggcggc
    34381 tgcagccagt gcgctcgccg tttgttgcgg cagtgcctgc tcgaactggt catcaatcac
    34441 cacaatcagt tcatccgggc cgcgcgatag cacatcttta cgccctttgg cgttggtcag
    34501 ggctttaccg cccacactta aggctgtcac ctgcgcctct gctgcctgtt gctttagctg
    34561 gcaagccgct tcaatagcgt tgagatcgta ttggcttatt ttggcatcgg ctttgctgaa
    34621 gtctaatgaa ccatcagcat tattgaccgc aatatcctgt tcatcaggca cgcacttata
    34681 gcaagtaata atcttcattg catctccaga aatcatgaag gaaacgtcgt tcatatcggt
    34741 agtgttaatt acagatatca cgccctttaa tttggaaata aaactttaat caccaatatt
    34801 gaaaatgtca cacgcagata aatccaactt tcaatattgt taagttcctc accaatattg
    34861 aaaacatggc gtagcaaaaa gaaattttca atattgtttt atagatcacc aatattgaaa
    34921 gtcagatgct attttttacc cacaaaagaa cataacaaac tgattaataa ccaaaattaa
    34981 cgaaaaacgt cctctattca ctcgaaacaa aaatgtgaca ccaatcacag aatacagctt
    35041 attgaatacc cattatgagt tagccattaa cgcgtccacg aggttaataa taattatatt
    35101 aaatgttaac aaaaataaaa caaacgggaa cacaaaataa atattcgttt tcacagtgga
    35161 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
    35221 cattaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcggcgaatg
    35281 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
    35341 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
    35401 gtttaggtaa cgaaccgcca gaatttagca ccgccagttg gatctttatg atgttcgcct
    35461 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
    35521 ccccgccgtt tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca
    35581 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
    35641 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
    35701 tggtaggtga aaaacacgcc aaagggttgt tcggtaccat cgtcgacaac ttctatctcg
    35761 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
    35821 gtatgcaatg gttgtttgac attccgcata ccctgcaact ggacgctatc atcattacct
    35881 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
    35941 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc atcgtcagcg
    36001 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
    36061 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
    36121 tgttctactg ggcatggtgg gtgatttacg ccatccagat gagtatcttc ctcgcccgca
    36181 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgatgggg ctgacagcat
    36241 ccacctggat cctgtggacc gtgctcggta gtaacactct gctgttgatg gataaaaaca
    36301 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc atcgaaacct
    36361 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
    36421 ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtctact tgccgcgaag
    36481 tacgcgatgg tgaagaacca ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca
    36541 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
    36601 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
    36661 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
    36721 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
    36781 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
    36841 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
    36901 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
    36961 caacctatgt actgtaccag ctgccgggcg ggttcaatac cttcctgcgc gaaggcacac
    37021 aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag
    37081 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
    37141 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagtagc gcctacaccc
    37201 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
    37261 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgagaag ctcggtctgc
    37321 gtatggatag ctgctgtgaa atcacctttg acgatgtgga actggacgag aaagacatgt
    37381 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc
    37441 tggtagccct gaccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg
    37501 ccaaccagcg cgtgcagttt ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat
    37561 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
    37621 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
    37681 atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
    37741 gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtt tccggaggat
    37801 ctgacgaaat gcagatcctg acgctgggtc gtgcggtgct gaagcaatac cgctaagtcc
    37861 gtgcgctgcc tgatgcgacg ctgacgcgtc ttatcaggcc tacgggagca cgaatgtaag
    37921 ccggataagg cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct
    37981 aataatggat catctaccca tgccgaaatt cggaccgctg gccggattgc gcgttgtctt
    38041 ctccggtatc gaaatcgccg gaccgtttgc cggacaaatg ttcgcagaat ggggcgcgga
    38101 agttatttgg atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc
    38161 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
    38221 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc
    38281 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
    38341 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
    38401 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
    38461 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
    38521 ggcgctggca gcactgcata aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
    38581 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
    38641 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
    38701 taaatgtgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
    38761 ctttaaagat attggcctcg cacatctact cggtacgccg gaaatcccgg aaggcactca
    38821 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
    38881 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
    38941 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
    39001 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
    39061 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcacatggca tggacacggc
    39121 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg
    39181 tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc
    39241 ccaataacaa acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc
    39301 tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg
    39361 aatccagcgg cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc
    39421 gcacggcaaa cctgttttat acgctgggga ttcgcaaagg caacaaggtt gcactacatc
    39481 tcgacaactg cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta
    39541 tggtgccgat taacgcccgc ctgttacgcg aggaaagcga gtggatcttg caaaatagcc
    39601 aggcgtgcct gctggtgacc agtgcgcaat tttatcctat gtatcaacag attcagcagg
    39661 aagatgccac tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg
    39721 gagtgagttc atttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc
    39781 cgctatcgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga
    39841 aaggtgtggt gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt
    39901 gtgcactgcg tgacgatgac gtctacatga cggtaatgcc tgcgtttcat atcgattgcc
    39961 agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat
    40021 acagcgcccg cgccttctgg ggacaggcgc agaagtaccg cgccaccatt accgaatgta
    40081 ttccgatgat gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc
    40141 gcctgcggga agtgatgttt tatctcaact tgtcggagca ggaaaaagac gcattttgtg
    40201 aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta
    40261 tcggcgatcg ccctagcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct
    40321 acgaagcgga gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa
    40381 tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag
    40441 ccactgcgaa agtgctggaa gccgacggct ggctacatac cggcgatacc ggataccgcg
    40501 acgaagaagg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg
    40561 agaatgtctc ctgcgtggag ctggaaaata tcatcgccac ccatccgaaa attcaggaca
    40621 tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc
    40681 tgaatgaagg tgaaacattg agcgaagagg aattttttcg cttctgcgaa caaaatatgg
    40741 cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg
    40801 ggaaaataat tagaaagaat ctgaaataaa tgaaacgtca gggaactacc ctgccagcta
    40861 ataatcacgc cctaaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctaaaaaagc
    40921 aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg
    40981 aaattaccct tgatcgccca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg
    41041 gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg
    41101 ccggagagaa attcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac
    41161 cggatgctga ctttggcccg ggtggttttg cgggattaac cgaaattttc aatctcgaca
    41221 aaccagttat cgcagctgtg aacggctacg cctttggcgg cggtttcgaa ctggcgctgg
    41281 cggcagattt tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaaactgg
    41341 gcatcgttcc tgacagcggc ggtgtgctgc gtctgccgaa gatcctgccg cctaccatcg
    41401 tcaatgaaat ggtgatgacc ggcagacgaa tgggcgcaga agaggcgctg cgttggggga
    41461 tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggctcagc
    41521 agctggttaa cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca
    41581 gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc
    41641 catcggttct gcattcggaa gatgccattg aagggccgct ggcgtttgcc gagaagcgcg
    41701 atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc
    41761 ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt
    41821 gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat
    41881 cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga
    41941 caccatcgtt ggggaaaacg gccatatcgg gcatggagcg atcctgcatg gttgtgtgat
    42001 tggtcgcgat gcattggtcg ggatgaacag cgtaattatg gatggcgcgg tcattggcga
    42061 agagagcatt gttgccgcaa tgagctttgt caaagcaggc tttagtggcg agaaacgcca
    42121 gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg
    42181 gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca
    42241 tgaaacgcag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga
    42301 tgtgacgcct aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa
    42361 catcgtatct ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc
    42421 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
    42481 atacgcggtg tctttcttac gccgactaat ttttcccgac tgttattgcg caggcgcgcg
    42541 taaatttgct catcgatatc gaccacttta accagtcgct gacactggca tccccgccct
    42601 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
    42661 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
    42721 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
    42781 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
    42841 cgttttgaag caattaaaca agaccatatt aaaattgtgg atatatcgat agtatgcaac
    42901 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
    42961 aaagatttac ttaatgggca aaaaaaaaac ggtcctcatc agagaaccgt attagttatt
    43021 cgatcaaatt agcggatgaa aaatatccgc cacgacacgc tattatttga tctgtgcgtg
    43081 catttcctgc accgaaatta ctttttcagt cgcatcggca ttcagcgcca tcgcggtagc
    43141 gaaaccaccg ttcagggtgg tgtcataatg cactttatat tgcagcgcac tgcgacggat
    43201 cacgcgggag tcttcaatcg cacgacggcc tgaggtggtg ttgatgatgt aggtatattc
    43261 gccattcttg atacggtcct gaatgtgcgg acggccttca tgcaccttgt ttaccagacg
    43321 cggattgata cccgcttcgc ccagcacaat cgccgtgccg tgggtcgcat ccagctcgaa
    43381 gccctgtttc agcagttttg ccgccaggtc caccacgcgt tctttatcgc cttcgcgcac
    43441 ggaaagcagc gcacgaccgt gtttcttcat ggtggagttg ctgcccagct gcgctttggc
    43501 gaacgcttca gcgaaggtgc ggcccacgcc catgacttcc ccggtagagc gcatttctgg
    43561 ccctaacagc gggtcaacgc ctgggaattt attgaacggc agcaccactt ctttcaccga
    43621 gtagtacggc ggaataactt ctttggttac gccctgctca gccagcgatt tgccagccat
    43681 cacacgcgcc gccacttttg ccagcggtac gccggtggct ttggagacga acggaacagt
    43741 acgcgccgca cgcgggttta cttcaatcag gtaaacttcg ttgtttttca ccgcaaactg
    43801 cacgttcatc agaccgcgca cctgcaattc gaaggccagt ttctgcacct gctggcgcat
    43861 cacatcctga atttcctgac ttaaggtgta cgctggcagc gaacatgcgg agtcaccgga
    43921 gtgcacgccc gcctgctcga tgtgctccat gatgccgcca atcagcacca tttcgccgtc
    43981 gcagatggca tccacgtcaa cttctactgc gtcatcaagg aaatgatcca gcaacactgg
    44041 cgcatcgtta gacacgctaa ccgccgtctg gaagtagcga cgcaggtcag cttcgtcata
    44101 gacgatttcc atcgcccgac cgccgagaac gtaagacgga cgcaccacca gcgggtagcc
    44161 aatctctttt gccttctcaa ctgccatttc aatagcagta acggtggcgt tcgccggttg
    44221 tttcagtttc agacggtcaa ccgcatgctg gaagcgttca cggtcttccg cacggtcgat
    44281 agcatccggg ctggtgccga taaccggtac gccagcggct tccagtgcac gcgccagttt
    44341 cagcggggtt tgaccgccgt actgaacgat aacgcctttc ggcttctcaa tacgcacgat
    44401 ttccagcaca tcttccagag ttaccggctc gaagtagagg cgatcggaag tgtcgtagtc
    44461 ggtagagacg gtttccgggt tacagttaac cataatggtt tcgtaaccgt cttcacgcag
    44521 cgccagcgag gcgtgtacgc agcagtagtc gaattcgata ccctgaccga tacggtttgg
    44581 accgccgcca agcaccatga ttttttcacg gtcggtagac ggattcgctt cgcactcttc
    44641 ttcataagtg gagtacatgt aagcggtgtc ggtggcaaac tctgccgcac aggtatccac
    44701 gcgcttgtag accgggtgca ggtcatattg gtcacgcagc ttacggattt ccgcttcgcg
    44761 tacgcccgcc agttttgcca agcgcgcatc ggcaaagcct ttgcgtttca gctggcgcag
    44821 gaattcagcg ttcaggccag tgatgcccac ttccgccact ttctcttcca gacgcaccag
    44881 ttcttcaatc tgtaccagga accagcggtc aatgttagtc aggttgaaga cgccgtccac
    44941 agacaggccc gcgcggaaag catcggcgat gtaccagata cgctctgcgc ctgcgtcttt
    45001 cagttcgcga cggattttgg ttaacgcttc cgggtcatcc aggctcactt tcgggtcgaa
    45061 gccagttgcg cccacttcca ggccgcgcag cgctttttgc agggattcct gctgcgtgcg
    45121 accaatcgcc atcacttcgc caaccgattt catctgagtg gtcagacggt cgttagcacc
    45181 ggcgaatttt tcgaagttga agcgaggaat tttagtaacc acatagtcga tggacggctc
    45241 gaaggaggcc ggagtacgtc cgccagtgat gtcgttcatc agttcgtcta gggtgtaacc
    45301 caccgccagt ttcgccgcca ctttagcaat cgggaaaccg gtcgctttcg acgccagcgc
    45361 cgaagaacgg gacacgcgtg ggttcatttc gataacaatc aggcgaccgt ttttcgggtt
    45421 caccgcaaac tggacattgg aaccaccggt ttcaacgccg atttcacgca gcaccgccat
    45481 cgaggcgtta cgcatgattt gatattcttt gtcagtcagc gtttgggctg gcgcgacagt
    45541 gatggagtca ccggtgtgga tgcccatcgc atcaaagttt tcgatagagc agacgatgat
    45601 gcagttgtcg tttttatcac gcaccacttc catctcgtac tctttccagc cgatcagcga
    45661 ctcatcaatc agcaactctt tggtcggaga gagatccaga ccgcgggcgc aaatttcttc
    45721 aaactcttca cggttataag cgataccgcc gccgctaccg cccatggtaa aggatgggcg
    45781 aataatgcac gggaagccca cgtcagcggc aacagccagc gcttcttcca tcgtgtgtgc
    45841 gataccggaa cgcgcggttt ccagaccaat tttcttcatc gctacgtcga aacgacggcg
    45901 gtcttctgct ttatcaatcg catcggcagt ggcaccaatc atggtgacac cgaactcttc
    45961 caacacgccc tgacgttcca gctccagcgc gcagttcagc gccgtctgac cgcccatggt
    46021 tggcagcacc gcgtccgggc gctctttttc aataatcttg cgtaccactt cccagtgaat
    46081 cggctcgatg taggtcgcat cggccatttc cgggtcggtc atgatggtcg ccgggttgga
    46141 gttcaccaga atgacgcggt aaccctcttc gcgcagggct ttacatgctt gcgcgccaga
    46201 gtagtcaaac tcacacgcct gaccgataac aatcgggccc gcacccagaa tcaggatact
    46261 ttttatatct gtacgttttg gcatggctct tttactcctg attacttagc ggttttacgg
    46321 tactgctcaa ttaacttgat aaagtggtcg aacaacggcg cggcgtcgtg tggaccaggg
    46381 ctggcttcag ggtgaccctg gaagctgaac gccggtttat cgatacgatg aatgccctgt
    46441 aacgtaccgt cgaacaggga tttatgcgtg acacgcaggt ttgcaggtaa tgttgcttca
    46501 tccaccgcaa aaccgtggtt ctgggcggtg atcatcacca cgtttttctc cacatcttta
    46561 accggatggt tgccgccgtg gtgaccaaat ttcattttga cagtcttcgc accgctcgcc
    46621 agcgccagca gctgatgacc gagacagatg ccgaataccg gaatatcggt ttcgaggaac
    46681 ttctggatgg cggtaatggc gtaatcgcac ggggccgggt cgccaggacc gttggagagg
    46741 aagatgccgt ctggattcat tttcagcaca tcttccgcag aagtttgcgc cggaacgatg
    46801 gtcaggcgac agcctctatc caccagcatc cgcaggatgt tgcgcttggc accaaaatca
    46861 taagccacga cgtggaacgg cagctcgtct tcttttttcg cttctggcag gccgccggtc
    46921 aacgtccagc tcccttgtgt ccagctatag gcttctgcgg tggtcacttc ttttgccaga
    46981 tccatgccgt tcagacctgg gaacgcgcgg gctttttcta acgccagcgc cgcatccggg
    47041 ttatcgcctg cgataatgca gccattctgt gcgcctttct cgcgcagtaa acgcgtcagc
    47101 ttacgggtat cgatatcggc aatcgccacg atgttatggc gcttcaggta agaagagagg
    47161 tcttcggtat tacggaagtt gctggcaatc agcggcaggt cgcgaatcac cagaccttgt
    47221 gcatgtacct gagaagattc ttcatcggcg tcattagtgc cgacattgcc aatatgggga
    47281 taagtaagag taacgatttg acgagaatag gaaggatcag tgaggatttc ttgataaccg
    47341 gtcattgaag tattgaaaac gacttcccca accgccgaac ctgttgcccc tatggcccga
    47401 ccgtgaaact gggttccgtc ttccagaacc aatagcgctg acttaatcaa aacacctcca
    47461 tagaatattc actcacttta tttgcatatt aattcacagt gatgacgtaa atcaatgcaa
    47521 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
    47581 tcaacttaat ggcaggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
    47641 aagcgacaaa aacatcatct aacctgttct ggaaaacgct tgcgcagcaa gaagattgca
    47701 tgaaataaaa acgagcagga aaaagtggac caaatggtca aaatttacat taaaacaaca
    47761 aaaatagaag acgaaacatg taatttttgg cagttataga aaatttaaaa gcataacacc
    47821 aaaaataaaa gggctaaaaa ttaaagccct ttataattaa taaattaaat gtgttacttt
    47881 tgcatcataa taaacatttt gtggttacaa attattgaga tcaagcacat ctcgcatatc
    47941 aaaaagacca ctttccttac cactcaacca caaagccgat cttaccgcac cgttagcaaa
    48001 tgtcatgcgg ctggacgcct tatgggtgat ctccagacgc tcgccaatat cggcaaacat
    48061 cgcggtatgt tcgccaacga tgtcacctgc acgcacggtg gcaaaaccaa tggtaccagg
    48121 cacacgttca ccggtatggc cttcacgact gtagaccgcg caatctttca gatctttatc
    48181 aagggcgtgg gcgatcgcct ctcccattgc cagtgcggtg cctgacggcg catcaacttt
    48241 atgtctatga tgtgcttcaa taatttcgat atcggtgtag tcacccatca ctttggctgc
    48301 tttctccagc agcttaagca tgacgttaac gccaacgcta aaattggcgg caaagacaat
    48361 cgcaatatcg gcggcggcgt cacgaattgc ctgtttaccg gcttcgtcaa accccgtagt
    48421 gccgatcacc atccctttgc catgctgccg acaaaaagcg agatggttca gcgtaccttc
    48481 cggacgggta aaatcgataa acacatcaaa atcatctttt atcgcatcaa gactgctttg
    48541 cacggtaacg cctgttttcc ctgcaccagc cagctcaccg gcgtcgctgc ccagtaaaga
    48601 agatccttcg cgctccagcg ccgcgcccag ctgcacgcct tctaatgcca gtgccgcctg
    48661 aatcaactgg cggcccatac gccccccggc tcccgcgatg gcaacgcgga tgtttgcatc
    48721 atgcatagct attctctttt gttaatttgc atagaccgtt ttcagagtaa ccagcgcatc
    48781 gctacgccgc cagccaaaaa cgctgataat tagaaattaa tgataaacag ggagggatat
    48841 cagtgaaagg catgagtcga tgaatgactg catgccgata acatgtgaca ggttacggca
    48901 ccagagccag cacctcagcc acccactgtt ggaagccttt tacattcaga tccaatgcca
    48961 cctgtacatt ggctggtttg cccaggcaac cgtcgatatc aaccaccgtc gtgcctgagg
    49021 taaattcgcc ttgagtttct accgcgacaa aacacggctt gagggtgaat aagtccgggc
    49081 gcaccagcca ggcgatggcg cagagatcgt gcattcgcag accgctttgc atactgccgc
    49141 tacggtagtg gctaaacagg gcgtggagca tttttccggt gcgattcaac tccggtaaag
    49201 tagcgagata gtcaggggtt aatattgcct gattggtgac atccagaccg cacatgacaa
    49261 tttcgatgcc gctacggaag acacaggcag ccgcttctgg atcggcagcg atattaaact
    49321 cggcatttgg tgtacagttg ccgcgaccag cagaaccgcc catgatcacc agacgacgaa
    49381 tatacggttt acattccggg cattgtaaaa gtaacagcgc aatattggtt aacgggccga
    49441 tggccaccag ggtaacgggc tctggtgcac gcatcagggc atcccgaatc gccagaaacg
    49501 ccggtatccc gagtggcttt cggttgtgct caacaaagtc gtagccagcc attcccgatt
    49561 cgccgtgcac agatgctgca tcacgcggtg cgcgtaccag cggcacagcg gctccctggg
    49621 cgagcggaat ctccgcattc cagaaatgca gcagttgcag ggcattgcgg gtagttttct
    49681 caaccgagac attacccgcg acggtggtca tcagttgcag gtcgagttcg ggggcaaaaa
    49741 tcgcggcggc aatggcgacg gcatcgtcaa tgccggggtc agtatcgagg aagataggta
    49801 aacgcatgtt ttcttcataa aaaatgccgg taacaagacc ggcattttcg cataacttag
    49861 gctgctaatg acttaatcga cttcacgaat atcgacacgc agctctttcg gcacttcgaa
    49921 aacaatattt tcttcacggc cttccagcgg aatggcttca ccaccaccca actgctgcaa
    49981 acgtgccacc acattctgca ccagaatatc cggagccgat gcgcccgcag tcacgccgac
    50041 gcatttaacc tctttcaccc actcttcctg gatatctttc gcatcgtcaa tcaaaaacgc
    50101 gtgtttgccc atacgctggg ccagctccgc cagacggttg gagttggagg agtttttcga
    50161 accgaccacc aacacaactt ccgcctgttc tgccagggcg cgtaccgctt cctgacggtt
    50221 agtcgtggcg tagcagatgt catctttgcg cggaccgaca attttcggga agcgtttacg
    50281 cagcgcgtcg atcacatcag acgtatcatc caccgacagc gtggtttggg tcataaagga
    50341 gagcttctct tcgtttttga ccgtcagttt ccacacatcg tctggcgatt cgaccagata
    50401 cattccccct tcggggttgc tgtactgccc catcgtccct tccacttccg ggtgcccggc
    50461 gtggccgatg agaatagatt cttcgccacg gcgactggcg cgggcaactt ccatatgcac
    50521 tttggtcacc agcggacagg tggcatcaaa caccgtcaaa tcgcggcttt tcgcttcgtt
    50581 acgaaccgcc tgagaaacac cgtgtgcgga gaaaatcagg atcgcgccgt ccggcacttc
    50641 gctaatctgc tcaataaaga tagccccacg ctcgcgcaag ctatcgacca cgtagcggtt
    50701 atgcaccact tcgtgacgga catatatcgg tgcgccgtaa atggccagcg cgttttcaac
    50761 aatgctgata gcgcggtcta ccccggcaca aaaaccacgc gggttggcca acaggatctg
    50821 catgttacgc ctccagtgcc ggatcgattt ccagcacttc aatatcaaaa tgaacggtct
    50881 gcccggccag cggatggttg aaatcaacgg taatggagtc gccgttaatt tcgcggatca
    50941 caccaggcat ctcactgcca tccattgcgg taaaaagcat gattgcgcca atttctggct
    51001 cgcctgcatc cataaattca cggcgggaga agtactgaat caggtccggt gacggcacgc
    51061 caaacgccgc atctggctcc agcgagaagg tggttttgtc gcccactttc agccccagca
    51121 ggtgttgctc cagcccttca gaaagagaag catcgcccag gcggaacagc gccggtttac
    51181 cgttgttgcg agtagactcg gcggtggtgc catcgtcgag ttttagcgtg aagtgcacca
    51241 ggacggcgct attgctctgt acagattcag acatgaaggt ttgctcttat ttttgaggat
    51301 ttttgccgga tgcggcctac gatttcgtag caatctgtag gccgcatccg gcagggttta
    51361 ttattgtttt ttcgctttgg aaggtaaaaa cccttccagc acgatcagtg ccgcaccgac
    51421 acaaatggca gtatcggcaa ggttgaaggt ggcgaagtgc cagtcgccga cgtagaagtc
    51481 gatcatatcg acaacgaagc cgtgccacag gcggtcgaac aggttgccca gcgcgccgcc
    51541 aataatcagc gcgtaagcga tattgtttag cttctgcgtg gccttcgagc gatacatcat
    51601 cactgccagg agcacgctaa taccaatcgc aataccggca aagaaccaac gctgccagcc
    51661 gccgctatcg gcaaggaaac taaacgccgc gccatagtta cgcgcataat gcagattaag
    51721 cgacgggaac agcgggaccg tatcccccag agcaaagttc tggaggatca ggtatttgct
    51781 gcccagatcg ataatcagca cgactaccac cagccacagc cagcgtagcc ctgttgaaca
    51841 gatcgattga ctcatcaggc aaacttacgt ttttcaccgt caccggcgac gttgctgaca
    51901 cagcggccgc agatttctgc gtgttccgcc accttgccga catcctgggt gtagtgccag
    51961 cagcgtgggc acttctcacc ttcggcttta ctcaacgcga ctttcagccc tttgagtact
    52021 tcgctctgct gagcatcagc aggtgcgtcg ttatagtctg caacggtagc gccggaggtc
    52081 aacaggacaa atcgtaattc atcgcccagc gcggtcagtt tcgccgccag ttccggttct
    52141 gcatacaagg ttactgccgc ttccagcgag ccacccactt tcttgtcggc acgcgcttgc
    52201 tcaatgactt tgttcacttc gccacgcact ttcaacagct cgtcccagaa cgcatcgttc
    52261 attgcttcac tgtctgccag accaaacagg ccttcgtacc actcaccggt gaagacgtat
    52321 ttttcacgtt cgcccggcag atagccccac acttcatcag cggtgaagga gaggattggt
    52381 gccatccagc gcaccagcgc ttcagcgatg tgatacagcg cagtctggca gctacgacgc
    52441 gccacgctat ccgctttggc ggtgtactga cggtctttga tgatgtcgag gtagaaggaa
    52501 cccatctcaa cggagcagaa gcgcatcaga cgctgtacca cttcgtggaa atcgtatgct
    52561 tcgtacgcct tgaggatgtc ttcctgtgcc gctttcgcac aacctacggc ccagcgatcc
    52621 agtaccacca tctcttccgg tttcaccata tcttttgctg gatcaaaacc attcaggttt
    52681 gccagcagga agcgcgcggt gttacggata cgacgatagc tatcggcagc acgtttcagg
    52741 atctcgtcag aaacggccat ttcaccggtg tagtcggttg atgccaccca cagacgcaga
    52801 atatccgcgc ccagtttgtt catcacatcc tgcggcgaaa cggtgttgcc gatggattta
    52861 gacatcttgc gaccctgacc atccacggta aagccgtggg tcagtacctg acgatacggc
    52921 gctttgcctt tcatcgcggt ggagatcatt agggaagaca tgaaccaacc acggtgctgg
    52981 tcagaacctt ccagatacat gtccgctgcg tgaccggcaa attccggacg cacgtcaaca
    53041 acagaagagt gggtagatcc ggagtcaaac catacgtcga gagtatccgg cactttcaca
    53101 tactggtcag cttcgtcgcc gaggatctct ttcgcatcga gatcccacca cgcctggatg
    53161 ccgtcaactt caacgcgttt agccacttct tccatcagtt cgagggtacg cggatgcagc
    53221 tcttccgtgt ctttgtgcac gaacagtgac atcggtacgc cccaggtgcg ctgacgggag
    53281 atacaccagt caggacggtt agcaaccatc gactcgatac gcgcctggcc ccagtccggg
    53341 atccactgca cgcctttgat ctctttcagt gactgcgcac gcagaccttt ctgatccatg
    53401 ctgacgaacc actgcggtgt cgcgcggaag atgatcggcg ttttgtgacg ccagcagcac
    53461 ggatagctgt gctgcatttt ctcaacgtgc agcagcgcgc ctttttcctg cagcagcgca
    53521 acgacgatgt cgttcgcttt gaagacgttc acgccatcca gcgtcggata agtgcccggc
    53581 agataagtgc cgtccgggcc aaccgggtta gcggtttcca ggccgtattt ctgaccgatc
    53641 acatagtcgt ccgggccgtg gccaggcgcg gtgtgaacgg caccggtacc ggcatccagg
    53701 gtaacgtgat cgccgaggat tgccggaacg tcgaagccca taaacggatg ggtaaagcgc
    53761 agcagctcaa gctctgcacc ttttaccgtg ccgagaatgg tgtaatcggt cacgccgata
    53821 cgctgcatta cgctttcaac cagatctttc gccagaatcg cggcctgacc gtcgatctgc
    53881 accagcgcat agtcgaaatc aggtgcaata gagattgcgc ggttcgcagg caaagtccac
    53941 ggcgtagtgg tccagatcac cagcgagatt gggccgttaa cgttgcttac gccaaatttc
    54001 gctttcagcg catcctgatc gaccgcctgg aaagcgacgt caatggacgg agaagttttg
    54061 tcgtaatact caacttccgc ttctgccagt gcagaacggc agtctacgca ccagtgcacc
    54121 ggcttcgcgc ctttgtgcag gtgaccattg ccgatgattt tgcccagcgc gcggatgatg
    54181 ttggcttcag ttttgaagtc catagtcaga tacgggtgcg accagtcgcc cagcacgccc
    54241 agacggataa agtctttgcg ttgaccgtca acctgggtcg ccgcgtattc gcggcacttg
    54301 gcgcggaact cggcggcggt gaatttctca cccggcttac cgtattcttg ctctactttc
    54361 agctcgatcg gcagaccgtg gcagtcccag ccaggcacat acggcgagtc ataaccggaa
    54421 agccctttgg acttcacgat aatgtctttc agaatcttgt taaccgagtg accaatatga
    54481 atgctgccat tcgcataagg agggccatca tgcagaatga aggttttttt gcctttttta
    54541 gccgcacgga tgatgccgta cagatcatca tcagtccaac gcgccagcat tcccggttcg
    54601 cgcttggcga gatcgccacg catcgggaac cctgtttccg gcaaattcag ggttgattta
    54661 tagtcactca tcagattctc ggttccgtgt ttcggtttga ttacataaca ggcttaagcc
    54721 ggttttgtta gcccaaaaaa ttcgcgggcg gttaattcat cacgcgcaat ctgcgctttc
    54781 agttcgtcca gcgacgcaaa tcgctgctca ttgcgtattt ttttacgcag cactacttgt
    54841 atatggcgac cgtaaaggtc cattgcaaca tctaacaaat gcacttccag ctgctggcga
    54901 ataccggcaa ccgttgggcg tgttccgatg tttgccacgc cgggtaacgg cttttcaccg
    54961 aggcccagca cttctaccgc ataaacccct ttcaccgggg aaacctgacg gcgtagcggt
    55021 acattcgccg tcgggaaacc tatagtgcgc cctaattcat caccgtggac tacacgcccg
    55081 gagatggcaa acgggtgccc cagtaaactt tctgccagag ccagattgtc atcagccagc
    55141 gcctgacgca cggcggtgct gctgatacgc acaccacctt cgcaaaaagt ttgcgtactg
    55201 gtgatatcga agccgtattc catgccagct ttctgtaata acaagaaatc gccttcacga
    55261 ccagcgccaa agcggaaatc atcacctacg gcaagaaatt ttacgcgcaa atgcttcacc
    55321 aaaagatcgc tgatgaaatt ttgcgcggtt aacgccgcaa aacgcctgtc gaaacgcacg
    55381 cacagcacgt aatcaacgcc acactctgca aggtaacgca gtttttcccg cagccgggtc
    55441 agtcttgccg gggctttatc ggtagcaaac agttccagtg gttgaggttc aaaaagcatc
    55501 accatcaccg gtaagttgcg cttgcgccct tcttcctgca agccctgtaa cagcgcgcga
    55561 tgaccgcgat gcacgccgtc gaaattacca atagtcagca cacacccttc ttgcggggcc
    55621 tggctgagat tatgtatgcc gcgtatcagc ttcatgtctg gctcaaaaca gtgaaaatcg
    55681 tccgagtata ccttgtacag cggtcaaggt taaccggcga ttgagtaccg aaatcagaaa
    55741 gaagtggctt ttcattgcca tggcgcaaat cacgggaaga aactgaccgc ctgctgcaat
    55801 ttttatcgcg gaaaagctgt attcacaccc cgcaagctgg tagaatcctg cgccatcact
    55861 acgtaacgag tgccggcaca ttaacggcgc ttatttgcac aaatccattg acaaaagaag
    55921 gctaaaaggg catattcctc ggcctttgaa ttgtccatat agaacacatt tgggagttgg
    55981 accttggcta atatcaaatc agctaagaag cgcgccattc agtctgaaaa ggctcgtaag
    56041 cacaacgcaa gccgtcgctc tatgatgcgt actttcatca agaaagtata cgcagctatc
    56101 gaagctggcg acaaagctgc tgcacagaaa gcatttaacg aaatgcaacc gatcgtggac
    56161 cgtcaggctg ctaaaggtct gatccacaaa aacaaagctg cacgtcataa ggctaacctg
    56221 actgcacaga tcaacaaact ggcttaatcg ccaatttgct gaagctttgt gaaaaagccc
    56281 gcgcaagcgg gtttttttat gcctgctgct tttgtgatgc gtgagagaac acactgccgg
    56341 ttgccagata gtcaaaagag acatgccgtt gtactatctg aactcttcgt ggcttaagaa
    56401 tcgtagaacc atcggctgat acattctgaa caagaatatc tactcaatct ccagactccc
    56461 ccacaaagaa tatggatatt gtgatacaca ttgaggtatt aaacttaaga ccactctatt
    56521 tttgaattaa attcctatat actttaacca ttcataaata tctcctcatt agac
//
LOCUS       DADLTF010000008        53210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.008, whole genome shotgun sequence.
ACCESSION   DADLTF010000008 DADLTF010000000
VERSION     DADLTF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.008"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(11..322)
                     /locus_tag="JN749_000501"
     CDS             complement(11..322)
                     /locus_tag="JN749_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HAY7367024.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="JN749_000502"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="JN749_000502"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="JN749_000503"
     CDS             701..1054
                     /locus_tag="JN749_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HAY7367025.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JN749_000504"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="JN749_000504"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HAY7367026.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="JN749_000505"
     CDS             complement(2810..3280)
                     /locus_tag="JN749_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367027.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JN749_000506"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="JN749_000506"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HAY7367028.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JN749_000507"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="JN749_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7367029.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JN749_000508"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="JN749_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HAY7367030.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="JN749_000509"
     CDS             complement(5283..8432)
                     /locus_tag="JN749_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HAY7367031.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JN749_000510"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="JN749_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HAY7367032.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="JN749_000511"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="JN749_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HAY7367033.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="JN749_000512"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="JN749_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HAY7367034.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JN749_000513"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="JN749_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HAY7367035.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JN749_000514"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="JN749_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HAY7367036.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="JN749_000515"
     CDS             14911..15288
                     /locus_tag="JN749_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HAY7367037.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="JN749_000516"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="JN749_000516"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAY7367038.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="JN749_000517"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="JN749_000517"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAY7367039.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     gene            18099..18428
                     /locus_tag="JN749_000518"
     CDS             18099..18428
                     /locus_tag="JN749_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAY7367040.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="JN749_000519"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="JN749_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAY7367041.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="JN749_000520"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="JN749_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HAY7367042.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="JN749_000521"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="JN749_000521"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAY7367043.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="JN749_000522"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="JN749_000522"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HAY7367044.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JN749_000523"
                     /pseudo
     CDS             complement(22933..23893)
                     /gene="aes"
                     /locus_tag="JN749_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /locus_tag="JN749_000524"
     CDS             24045..25349
                     /locus_tag="JN749_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HAY7367045.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="JN749_000525"
     CDS             complement(25482..27158)
                     /locus_tag="JN749_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HAY7367046.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGKIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /locus_tag="JN749_000526"
     CDS             complement(27396..28616)
                     /locus_tag="JN749_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367047.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSQGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /locus_tag="JN749_000527"
     CDS             28834..30486
                     /locus_tag="JN749_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7367048.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JN749_000528"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="JN749_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HAY7367049.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="JN749_000529"
     CDS             complement(31206..32000)
                     /locus_tag="JN749_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367050.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="JN749_000530"
     CDS             32138..32479
                     /locus_tag="JN749_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7367051.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JN749_000531"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="JN749_000531"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HAY7367052.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="JN749_000532"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="JN749_000532"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAY7367053.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="JN749_000533"
     CDS             36395..37687
                     /locus_tag="JN749_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7367054.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /locus_tag="JN749_000534"
     CDS             37812..38219
                     /locus_tag="JN749_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HAY7367055.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="JN749_000535"
     CDS             complement(38220..38678)
                     /locus_tag="JN749_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HAY7367056.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="JN749_000536"
     CDS             complement(38675..39592)
                     /locus_tag="JN749_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HAY7367057.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="JN749_000537"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="JN749_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HAY7367058.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="JN749_000538"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="JN749_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HAY7367059.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JN749_000539"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="JN749_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HAY7367060.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JN749_000540"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="JN749_000540"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HAY7367061.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JN749_000541"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="JN749_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="JN749_000542"
     CDS             43551..44237
                     /locus_tag="JN749_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7367062.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46648
                     /locus_tag="JN749_000543"
     CDS             44234..46648
                     /locus_tag="JN749_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7367063.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JN749_000544"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="JN749_000544"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HAY7367064.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JN749_000545"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="JN749_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HAY7367065.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="JN749_000546"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="JN749_000546"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HAY7367066.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="JN749_000547"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="JN749_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HAY7367067.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="JN749_000548"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="JN749_000548"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HAY7367068.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALNSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53210
                     /locus_tag="JN749_000549"
     CDS             52938..>53210
                     /locus_tag="JN749_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HAY7367069.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12821 a        13706 c        14019 g        12664 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgtgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcatca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caatatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacta ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacgg ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgttgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg atagggtaaa cagttcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgattttccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggccctg ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgtgggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atacggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctacaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 gggtcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atatccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acggacgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tgtaactatg attttcttca tgtgctagat gaggactgcc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aacaaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggaccaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt taaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctcaattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgatgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcactg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtagtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt
//
LOCUS       DADLTF010000009        52973 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.009, whole genome shotgun sequence.
ACCESSION   DADLTF010000009 DADLTF010000000
VERSION     DADLTF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52973
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.009"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..326
                     /locus_tag="JN749_000550"
     CDS             <1..326
                     /locus_tag="JN749_000550"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7367070.1"
                     /translation="GLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYN
                     LGHSTIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLK
                     HANHK"
     gene            complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN749_000551"
     CDS             complement(367..903)
                     /gene="hpt"
                     /locus_tag="JN749_000551"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAY7367071.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            1109..3499
                     /locus_tag="JN749_000552"
     CDS             1109..3499
                     /locus_tag="JN749_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HAY7367072.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHAFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(3546..5095)
                     /gene="cueO"
                     /locus_tag="JN749_000553"
                     /pseudo
     CDS             complement(3546..5095)
                     /gene="cueO"
                     /locus_tag="JN749_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            5261..5608
                     /locus_tag="JN749_000554"
     CDS             5261..5608
                     /locus_tag="JN749_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HAY7367073.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            5714..6580
                     /gene="speE"
                     /locus_tag="JN749_000555"
     CDS             5714..6580
                     /gene="speE"
                     /locus_tag="JN749_000555"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HAY7367074.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            6596..7390
                     /gene="speD"
                     /locus_tag="JN749_000556"
     CDS             6596..7390
                     /gene="speD"
                     /locus_tag="JN749_000556"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HAY7367075.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(7428..7790)
                     /locus_tag="JN749_000557"
     CDS             complement(7428..7790)
                     /locus_tag="JN749_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HAY7367076.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN749_000558"
     CDS             complement(7966..10563)
                     /gene="acnB"
                     /locus_tag="JN749_000558"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HAY7367077.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            10918..12771
                     /locus_tag="JN749_000559"
     CDS             10918..12771
                     /locus_tag="JN749_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HAY7367078.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNSITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQATASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(12842..14272)
                     /gene="lpdA"
                     /locus_tag="JN749_000560"
     CDS             complement(12842..14272)
                     /gene="lpdA"
                     /locus_tag="JN749_000560"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAY7367079.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPKAKKK"
     gene            complement(14597..16489)
                     /gene="aceF"
                     /locus_tag="JN749_000561"
     CDS             complement(14597..16489)
                     /gene="aceF"
                     /locus_tag="JN749_000561"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAY7367080.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPVAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(16504..19167)
                     /gene="aceE"
                     /locus_tag="JN749_000562"
     CDS             complement(16504..19167)
                     /gene="aceE"
                     /locus_tag="JN749_000562"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HAY7367081.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(19328..20092)
                     /gene="pdhR"
                     /locus_tag="JN749_000563"
     CDS             complement(19328..20092)
                     /gene="pdhR"
                     /locus_tag="JN749_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HAY7367082.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            20633..22006
                     /gene="aroP"
                     /locus_tag="JN749_000564"
     CDS             20633..22006
                     /gene="aroP"
                     /locus_tag="JN749_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HAY7367083.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(22049..22903)
                     /gene="ampE"
                     /locus_tag="JN749_000565"
     CDS             complement(22049..22903)
                     /gene="ampE"
                     /locus_tag="JN749_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HAY7367084.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(22900..23451)
                     /gene="ampD"
                     /locus_tag="JN749_000566"
     CDS             complement(22900..23451)
                     /gene="ampD"
                     /locus_tag="JN749_000566"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HAY7367085.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            23539..24432
                     /gene="nadC"
                     /locus_tag="JN749_000567"
     CDS             23539..24432
                     /gene="nadC"
                     /locus_tag="JN749_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HAY7367086.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNDKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            24635..25075
                     /gene="ppdD"
                     /locus_tag="JN749_000568"
     CDS             24635..25075
                     /gene="ppdD"
                     /locus_tag="JN749_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HAY7367087.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            25085..26470
                     /gene="gspE"
                     /locus_tag="JN749_000569"
     CDS             25085..26470
                     /gene="gspE"
                     /locus_tag="JN749_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HAY7367088.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAVQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            26460..27662
                     /gene="hofC"
                     /locus_tag="JN749_000570"
     CDS             26460..27662
                     /gene="hofC"
                     /locus_tag="JN749_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HAY7367089.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(27697..28740)
                     /gene="guaC"
                     /locus_tag="JN749_000571"
     CDS             complement(27697..28740)
                     /gene="guaC"
                     /locus_tag="JN749_000571"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAY7367090.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            28965..29585
                     /gene="coaE"
                     /locus_tag="JN749_000572"
     CDS             28965..29585
                     /gene="coaE"
                     /locus_tag="JN749_000572"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAY7367091.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLIENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            29585..30328
                     /gene="zapD"
                     /locus_tag="JN749_000573"
     CDS             29585..30328
                     /gene="zapD"
                     /locus_tag="JN749_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HAY7367092.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            30338..30535
                     /gene="yacG"
                     /locus_tag="JN749_000574"
     CDS             30338..30535
                     /gene="yacG"
                     /locus_tag="JN749_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HAY7367093.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(30635..31024)
                     /gene="mutT"
                     /locus_tag="JN749_000575"
     CDS             complement(30635..31024)
                     /gene="mutT"
                     /locus_tag="JN749_000575"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HAY7367094.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(31084..33789)
                     /gene="secA"
                     /locus_tag="JN749_000576"
     CDS             complement(31084..33789)
                     /gene="secA"
                     /locus_tag="JN749_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAY7367095.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKLGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(33851..34363)
                     /gene="secM"
                     /locus_tag="JN749_000577"
     CDS             complement(33851..34363)
                     /gene="secM"
                     /locus_tag="JN749_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HAY7367096.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(34594..35511)
                     /gene="lpxC"
                     /locus_tag="JN749_000578"
     CDS             complement(34594..35511)
                     /gene="lpxC"
                     /locus_tag="JN749_000578"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HAY7367097.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(35612..36763)
                     /gene="ftsZ"
                     /locus_tag="JN749_000579"
     CDS             complement(35612..36763)
                     /gene="ftsZ"
                     /locus_tag="JN749_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAY7367098.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(36824..38086)
                     /gene="ftsA"
                     /locus_tag="JN749_000580"
     CDS             complement(36824..38086)
                     /gene="ftsA"
                     /locus_tag="JN749_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAY7367099.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(38083..38913)
                     /gene="ftsQ"
                     /locus_tag="JN749_000581"
     CDS             complement(38083..38913)
                     /gene="ftsQ"
                     /locus_tag="JN749_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HAY7367100.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(38915..39835)
                     /locus_tag="JN749_000582"
     CDS             complement(38915..39835)
                     /locus_tag="JN749_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7367101.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(39828..41303)
                     /gene="murC"
                     /locus_tag="JN749_000583"
     CDS             complement(39828..41303)
                     /gene="murC"
                     /locus_tag="JN749_000583"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAY7367102.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(41357..42424)
                     /gene="murG"
                     /locus_tag="JN749_000584"
     CDS             complement(41357..42424)
                     /gene="murG"
                     /locus_tag="JN749_000584"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAY7367103.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNGWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(42421..43665)
                     /gene="ftsW"
                     /locus_tag="JN749_000585"
     CDS             complement(42421..43665)
                     /gene="ftsW"
                     /locus_tag="JN749_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HAY7367104.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(43665..44981)
                     /gene="murD"
                     /locus_tag="JN749_000586"
     CDS             complement(43665..44981)
                     /gene="murD"
                     /locus_tag="JN749_000586"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAY7367105.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(44984..46066)
                     /gene="mraY"
                     /locus_tag="JN749_000587"
     CDS             complement(44984..46066)
                     /gene="mraY"
                     /locus_tag="JN749_000587"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAY7367106.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(46060..47418)
                     /gene="murF"
                     /locus_tag="JN749_000588"
     CDS             complement(46060..47418)
                     /gene="murF"
                     /locus_tag="JN749_000588"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAY7367107.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIARTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEDCHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(47415..48902)
                     /gene="murE"
                     /locus_tag="JN749_000589"
     CDS             complement(47415..48902)
                     /gene="murE"
                     /locus_tag="JN749_000589"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HAY7367108.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(48889..50655)
                     /locus_tag="JN749_000590"
     CDS             complement(48889..50655)
                     /locus_tag="JN749_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HAY7367109.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(50671..51036)
                     /gene="ftsL"
                     /locus_tag="JN749_000591"
     CDS             complement(50671..51036)
                     /gene="ftsL"
                     /locus_tag="JN749_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAY7367110.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(51033..51974)
                     /gene="rsmH"
                     /locus_tag="JN749_000592"
     CDS             complement(51033..51974)
                     /gene="rsmH"
                     /locus_tag="JN749_000592"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAY7367111.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(51976..52434)
                     /gene="mraZ"
                     /locus_tag="JN749_000593"
     CDS             complement(51976..52434)
                     /gene="mraZ"
                     /locus_tag="JN749_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAY7367112.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        12527 a        14792 c        13099 g        12555 t
ORIGIN      
        1 tggggcttat caacaactgg ctgctgcata tccgcgatat ctggttcaaa catagctcat
       61 tgctcggcga aatgccgcaa gagcgccgtc tggatacctt gtgtgaactg aacgtcatgg
      121 aacaggtgta taacctgggc cactccacca ttatgcaatc agcgtggaaa cgcgggcaga
      181 aagttaccat tcacggctgg gcctacggca ttcacgacgg cttgctgcgt gatctggatg
      241 ttaccgccac caaccgcgaa acccttgagc aacgttaccg tcacgggatt tccaacctca
      301 agctgaaaca cgccaaccac aaataaaaat gccatgccgg atgcaacaca tccggcaact
      361 tcacacttac tcgtccagca gaatcacttt gccaatatac ggcagatgac ggtaacgctg
      421 tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg atcgagaaac cgataaattc
      481 aaccgggacg ttcacttcac gacgggacgg tttatccagc agcgtacaaa tcgccaacga
      541 cttcggttcg cgcaggctta agatctcacg cactttcgac agtgtattcc ccgagtcgat
      601 gatatcttca acaatcagca cgtccttgcc acggatatct tcatccagat ctttgaggat
      661 tttcacatca cgggtggtgg acatgccgct accgtagctg gaggcggtca taaagtcgac
      721 ttcatgagat acctgaactt cacggcacag gtccgccata aacataaatg agccacgcag
      781 cagacccacc agcaccatat cgctgccgct gtctttgtaa cgctcagtaa tctgacgacc
      841 cagttcggcg atacgcgctt taatctccgc ttcggggatc attacttcta cagtatgttt
      901 catatctcta accatatgat ttaaaaacaa atcacttcgc acgggcaggt tttggcaccg
      961 caacaaagcg caagacacgc agtataccag taaaactatt cattcgcgga atctgttaat
     1021 aaagctcgaa ttgtgatgac ggtcacacat gttaaaatct ataattaatt gctactaaaa
     1081 tattaatgaa ttgaaatggt gtctctttat ggcaattaac aatacaggct cgcgacgatt
     1141 actcgtcacg ctaacagccc tttttgcagc gctttgcggg ctgtatctac tcattggcgg
     1201 aggctggctg gtcgcgattg gcggctcctg gtactaccct atcgctggcc ttgtgatgct
     1261 cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg ctttggctat acgcagccct
     1321 gctgctcggc accatgattt ggggcgtctg ggaagttggt ttcgacttct gggcgctgac
     1381 tccgcgcagc gatattctgg tcttcttcgg catctggctg atcctgccgt ttgtctggcg
     1441 tcgcctggtc attcctgcca gcggcgcagt tgccgcactg gtggtcgcac tgctgattag
     1501 cggtggtatc ctgacctggg ccggatttaa cgatccgcag gagatcaacg gcaccttaag
     1561 cgccgatgcc acacctgccg aagctatctc ccccgttgcc gatcaggact ggcctgcgta
     1621 tggccgcaat caggaaggtc aacgcttttc accactgaag caaattcacg ccgataacgt
     1681 ccacaagctg aaagaagcct gggtgttccg tactggcgat gtgaagcagc cgaacgatcc
     1741 gggtgaaatc accaatgaag tgacgccgat taaagtgggc gatacccttt acctgtgtac
     1801 cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc aaagagaaat ggcattacga
     1861 tcctgagctg aaaaccaacg agtctttcca gcacgtaacc tgccgtggcg tctcttatca
     1921 tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg gattgcccgc gtcgtatcat
     1981 tcttccagtg aatgatggtc gcctgattgc gattaacgct gaaaacggca aactgtgcga
     2041 aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat atgccagaca ccaaaccggg
     2101 tctgtatgag ccgacttctc cgccaattat caccgataaa accatcgtga tggccggttc
     2161 agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg atccgtggtt ttgatgtcaa
     2221 caccggggag ctgctgtggg cttttgatcc gggcgcgaaa gatccgaacg caatcccgtc
     2281 tgacgaacac gcctttacct ttaactcacc aaactcgtgg gcaccagcgg cctatgacgc
     2341 gaagctggat ctggtctatc tgccgatggg cgtgaccacg ccggatatct ggggcggtaa
     2401 ccgcacaccg gaacaggaac gttatgccag ctcgattctg gcgctgaatg ccactaccgg
     2461 gaaactggcg tggagctacc agaccgttca ccacgacctg tgggacatgg atcttccggc
     2521 acagccgacg ctggcggaca tcaccgttaa tggtcagaaa gtgccgatta tttacgctcc
     2581 ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat ggcgaactgg tggttccggc
     2641 cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat tacgttaccc ctactcaacc
     2701 attctcggaa ctgagcttcc gtccgacgaa agatttgagc ggtgcggata tgtggggagc
     2761 caccatgttt gaccaactgg tgtgccgcgt gatgttccac cagatgcgct atgaaggcat
     2821 tttcacgccg ccatctgaac agggtacgct ggtcttcccg ggtaacctgg ggatgttcga
     2881 atggggcggg atttccgttg atccaaatcg tgaagtggca attgccaacc caatggcact
     2941 gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac ccgatggagc agccgaaaga
     3001 tgccaaaggc acgggtacgg aatccggcat tcagccacag tacggtgtac cgtatggtgt
     3061 cacgctcaac ccgttcctct caccatttgg tctgccatgt aaacagccag catggggtta
     3121 tatctcggcg ctggatctga aaactaatga agtggtgtgg aagaaacgta ttggtacgcc
     3181 gcaggacagt atgccgttcc cgatgccggt tccggtgccg ttcaatatgg gtatgccgat
     3241 gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt atcgccgcta cggcagataa
     3301 ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg tggcagggtc gtttaccggc
     3361 gggtggtcag gcaacaccga tgacctatga agtgaatggc aagcagtacg tggtgatctc
     3421 cgcaggcggt cacggttcat ttggtacgaa gatgggcgac tatattgtgg cttatgccct
     3481 gccggatgat gtgaaataac cataatgccc ggagaaatcc gggcatattt ccgaatacgg
     3541 tctttttata ccgtaaaccc taacatcatc cccgtatctt catgctccag cagatggcag
     3601 tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa acttcaccag cacttcactg
     3661 acattgcctt ctaccttaac ggtatctttc cagcccgcgc gatgagccgc tggcggtttg
     3721 ccattttctg acaagatacg gaactgcgtg ccgtgaatat ggaacggatg cagcatcatg
     3781 tcgcccacgc cagagataac ccaacgttcg tactgccctt tcgccgccgc aaacatcggc
     3841 ttattcatat caaacgcctg accgttgatt ttattggcat ggtggaaatc gaacttcccg
     3901 ccgtggttca tatgattcat attgccgtgc cccatatggc ccatcatctg gctgtgatcc
     3961 attccggcca tcgcctgatc gccatatttc tccattagca tctgcatccc catcatatcg
     4021 agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca gcccttccag cgaaggtaac
     4081 gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg cactaatagc aatcggctga
     4141 atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg ccatccccat ctggctgacc
     4201 ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct ccaccagcac ttcaaaacgc
     4261 tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt caggtagcag accaccgtcg
     4321 ctggcaatca catacagcgg gcgattgtcg ctggtggcga aattgagcga gcgggcatta
     4381 cagccattga gcaaacgcag gcgcagccaa ccacgcgggg cagcgtgttg tgggtagatt
     4441 gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg cggcggtcat cacatccagt
     4501 tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct gaacgatcac cggaacatca
     4561 tcgatacccc actgttttgg cagcattaat ttcaggatct cgtcatcttc aatcaccacc
     4621 agaccagcca gccccatcgc cacctgtcgc cggtcttgcc atgttgatgt ggatggaacc
     4681 agcaggtagc ggcaggttga tcaacgttca acgtcaccga gcgcttgcca cctggcggaa
     4741 taattccctg tgggccgccg tcgacgtcac ccggtacttc cagcccgtgc cagtgcaacg
     4801 tcgtctcttc cgccagttgg ttgtagatat caaccgttac cgctttgccg cgctgtaatt
     4861 tcaccgccgg ccccagcaga ttgccgttat agccccaggt agttgcagtt ttctcgccaa
     4921 aggtggactg acctgcgcca atagttaact gaatgcgatt acgggcatcg gtcgtgagca
     4981 aatcagggat cggtaacgtt gggcgttccg ccgcaaagac tgcacggctc cacagtggca
     5041 aggctgaagc cacacccagt gcgacggaat atttcaagaa atcacgacgt tgcatagtta
     5101 tttccttatt cttaagcagg caaaacaaaa tcaaacgttg agcatagtcc ttccccttac
     5161 gggaaggtca agccgcagac acaatttaat cgacaataat ggtcgtcgcc tcgctcgcag
     5221 tgtgctaacg tttatcttct ttaagcccct ggtagaagca atgaagacgt ttttcagaac
     5281 agtgttattc ggcagcctga tcgccgtttg cgcaaacagt tacgcgctca gcgagtctga
     5341 agccgaagat atggccgatt taacggcagt ttttgtcttt ctgaagaacg attgtggtta
     5401 ccagaactta cctaacgggc aaattcgtcg cgcactggtc tttttcgccc agcaaaacca
     5461 gtgggatctc agtaattacg acaccttcga catgaaagct ctcggtgaag acagctaccg
     5521 cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc aaagccctgg cccgcgattc
     5581 cttaagcctg cttgcctacg tcaaataatc cctgatacct ttttgtagaa atattgaccg
     5641 tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt ttttacgggt gttaacaaag
     5701 gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa acgctacacg accagtttgg
     5761 gcagtacttt gcggtagata acgttctgta tcatgaaaag accgatcacc aggatctgat
     5821 catttttgag aacgctgcat ttggtcgcgt aatggcgctg gatggcgtag tacaaaccac
     5881 cgagcgcgac gagtttatct atcatgagat gatgacccat gttccgctac tggcccacgg
     5941 tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt gccatgctgc gtgaagtaac
     6001 ccgacataaa aacgttgagt caatcacgat ggtggaaatc gatgcgggtg tcgtgtcgtt
     6061 ctgccgtcag tatctgccca accataacgc cggtagctac gacgatccgc gctttcaact
     6121 agtgatcgac gatggcgtca atttcgttaa tcaaaccagc cagacctttg atgtcattat
     6181 ctccgactgc accgatccta tcggtcccgg cgaaagcctg ttcacctcag cattttatga
     6241 aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc gcacaaaacg gcgtctgctt
     6301 tttacagcag gaagaagcca tcgacagcca tcgcaaactc agccattact tcagcgacgt
     6361 tggcttttat caggcggcga tcccgaccta ttacggcggt atcatgactt ttgcatgggc
     6421 gacagataac gacgccttac gccatctctc aaccgaaatt attcaggcgc gttttctcgc
     6481 ttctggcctg aaatgccgtt attacaatcc ggcaatccat acggcagctt ttgccttacc
     6541 tcagtatctg caagacgcac tggcttcaca gccgtcctaa ggagaagata agaaattgaa
     6601 aaaactgaaa ctgcatggct ttaataatct gaccaaaagt ctgagttttt gtatttacga
     6661 tatctgctac gccaaaactg ccgaagagcg cgacggttat attgcttata tcgatgaact
     6721 ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc tgttccatta tcggggccaa
     6781 tattcttaac atcgcccgcc aggattacga accacagggt gccagcgtca ctattctggt
     6841 gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca gaacaccccg gcccactgcc
     6901 agaaacggtg gttgctcatc tcgataaaag tcatatttgc gtacatacct acccggaaag
     6961 tcatcctgaa ggcggtttat gtaccttccg cgccgatatt gaagtctcta cctgcggcgt
     7021 gatttctccg ctgaaggcgc tgaattacct gatccaccag cttgagtccg atatcgtaac
     7081 cattgattat cgcgtgcgcg gttttactcg cgacattaac ggtatgaagc actttatcga
     7141 ccatgagatt aattcgattc agaactttat gtctgacgat atgaaggcgc tgtatgacat
     7201 ggtggatgta aacgtctatc aggaaaatat cttccatacc aagatgttgc ttaaagagtt
     7261 cgaccttaag cactacatgt tccacaccaa accggaagat ttaaccgaca gcgagcgcca
     7321 ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt tattacgggc gcaatatgcc
     7381 agctgtttaa cggctctggc ggagctccca ggctccgcca gatttattta cttctgctgc
     7441 acgaaattgc ggtaagccgc cacgacctgc agaaaatcct caacgccgca tagcgacagg
     7501 ctttcttcgt cgtagtagtt catcccctct tccatttcat cgccagcgaa ttccagttga
     7561 ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg tgtattcatg ccctgcccgt
     7621 tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt gttccacttc atcaagcaag
     7681 gccaggtttt ctttcacctc ttcattaaac cagtgcccga ccacttcatg ccccatggac
     7741 atacgcacct ttaccactcc ggtaatatcg cgcagaaatt cgtaatccat agtgtgttcc
     7801 tctgctcccg gcaatgcgcc gaaatcgcat cgcgccattg atgtaattat cgcagcctta
     7861 ccggagaaaa acagcggaac ggcgatggtt tagaaataaa aaatgcccgg gaatctcatt
     7921 caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt acttattaca ccgcagtctg
     7981 gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc tggttgaagt tcagataacg
     8041 ataagtatca acggcagttt tatctacttg cgcaacgtag gtctggtact cttccggcgt
     8101 cggcagtttg ccaatcagcg ccgcaacagc cgccagttct gcagaagcca ggaagacatt
     8161 cgcgccagta cccaaacggt tcgggaagtt acgggtagag gtggaaacca ccgtcgcacc
     8221 gtctgctaca cgcgcctggt tacccataca cagggaacag cccgggatct cgatacgagc
     8281 cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc agttgcgcgg cgtccatacg
     8341 ggttggcggt gccacccaca ggcgggtcgg caactgacct ttgtgcgcat ccagcagttt
     8401 accagcggca cggaagtgac cgatgttagt catgcaagaa ccgataaata cttcgtcgat
     8461 cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc ggatcgttcg gagcacacag
     8521 gattggctct ttaatctccg ccagatcgat gtcgatcact gccgcgtatt ccgcatctgc
     8581 atcggcttcc agcagctcag gattcgccag ccatttttcc atgccctgaa tacggcgttc
     8641 caaggtacga cgatcgccgt aaccttccgc gatcatccac ttcagcagga cgatgttgga
     8701 gctcaggtat tcgatgatcg gttctttgtt cagcttgata gtacaaccag cggcagaacg
     8761 ctcggcggac gcatcggtta gctcaaaggc ctgctcaact ttcagatccg gcagaccttc
     8821 aatttccaga atgcgaccag agaagatgtt tttcttgcct ttcttctcaa cggtcagcag
     8881 accttgtttg atcgcataca gcggaatggc gtgtaccaga tcgcgcaggg tgatgcccgg
     8941 ctgcattttg cctttgaagc gcaccagaac ggattccggc atatccagcg gcattacgcc
     9001 agttgcggca gcaaacgcca ccagaccaga acccgccggg aaagagatac cgatcgggaa
     9061 acgggtatgg gagtcaccac cggtaccgac ggtatccggc agcagcatac ggttcagcca
     9121 ggagtgaatg acgccgtcac ccggacgcag cgacacaccg ccacggttca taatgaagtc
     9181 cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga tacgccgcgg tgtggcagaa
     9241 agactgcatc accaggtcag ccgagaagcc caggcacgcc aggtctttca gttcatcacg
     9301 ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc attttcggtt cgcagtacgc
     9361 gcccggacga atgcctttca cgccacaggc acggcctacc attttctgcg ccagcgagaa
     9421 gccgcgatcg ctctcagcga catctttcgc ctgacggaac acatcactgt gcggcagacc
     9481 aagtgcttca cgagctttgg tggtcaggcc acgcccgata atcagcggaa tacggccgcc
     9541 agcacgcact tcatcaatca gcacgtcggt tttcagttcg aaggtcgcca gcagttcgcc
     9601 tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg tcaatcacgt cgcccatgtt
     9661 caggttagag acgttgactt cgattggcag tgcacccgcg tcttccatcg tgttaaagaa
     9721 gatgggtgca attttaccgc cgaggcacaa accaccgccg cgtttgttcg gcacatgtgg
     9781 aatatcatcg cccataaacc acagaacgga gttcgtggcg gatttacgcg atgaacccgt
     9841 acccacaacg tcaccgacgt acgccagcgg gaaacctttc tgttgcagag cttcaatctg
     9901 tttgatcggg ccaacaacac ctggctggtc tggctcaata ccttcacggg cgtttttcag
     9961 catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac gcatccggtg ccggagaaag
    10021 gtcatcggtg ttggtttcgc cagtgacttt aaagacggta acggtcagtt tttcagccag
    10081 cgccgggcga ttcaggaacc attcggcatc cgcccaggac tgcattacct gcttcgcata
    10141 ttcgttgcct gctttcgctt tctcttctac gtcatagaag ttatcgaaca tcagcagcgt
    10201 gtgagaaagt gctttggcag caataggtgc cagtttggca tcatccagcg cgtcgatcag
    10261 cggatgaatg ttgtaaccac cctgcatggt gcccagcagt tcgatagctt tttccggagt
    10321 cagcagaggg gatttagctt cgcctttagc gatagcagcc aggaagcctg cttttacata
    10381 ggcggcttca tcgacgcctg ggggaacacg gttggttaac agatctaaca ggaattcttc
    10441 ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg gccatttggt ttgcatccag
    10501 gggtttgggc gcaatcccct cagcggcacg ctcagctacg tgcttacggt attcttctag
    10561 cacgacggtt ctcctcgctc tcattgtcat agtgcggcag gcgattctct tcacgctcct
    10621 gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa cgggcagaat agcaggattt
    10681 tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa aagtggctga atcgttaagg
    10741 atggtctaga gattgtttcc ctccgtaaaa acttcgttaa ttcgcatggt gataatcacc
    10801 tttctcaacg aatcccaaga ttagtcaaaa tttaaactac cgcctcttta tactcggatt
    10861 cacagcacct gcgggtggca gttcgccgac cattgcgatt tccttgagat ccgaattatg
    10921 aaaatgactt tgccgtttaa accccatgtg ctggcgctaa tttgcagtgc cgggctttgt
    10981 gctgcctctg ccgggctata tataaaaagc cgcacagtgg aagcgcctgt agaaccgcaa
    11041 tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc ttcctgcaac ggtttccgca
    11101 cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca ctgcacaaat agatcaatgg
    11161 gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc aggtgctgat ggcatcaacc
    11221 tatccgacaa acgttgctca agcagtgcaa tggtcgcacg ataatccact taaacaaggc
    11281 gatgctgcta ttcaggcggt atctgaccag ccgtgggacg ccagcgttaa atcactggtg
    11341 gcctttccac aattgatggc attgatgggc gaaaacccgc aatgggtgca aaacctgggc
    11401 gatgcttttc tggcgcagcc gcaggacgtg atggactcgg tacagcgatt gcggcaactg
    11461 gcgcaacaaa ccggctcgct gaagtcatca accgaacaga aagttattac cacaacgaag
    11521 aaagctgtac cggtaaaaca gacagtcacg gcacccgtca taccatccaa taccgtttta
    11581 actgccaacc ccgtcattac agagccggca acaaccgtca tttccattga gcccgccaat
    11641 cctgatgtgg tctatattcc caactacaac ccaaccgtgg tttacgggaa ctgggccaat
    11701 actgcgtatc cgccggttta tctgccacca ccagccggag aaccgtttgt tgacagcttt
    11761 gtgcgcggat tcggctatag catgggcgtt gctaccacgt acgcactatt cagcagcatc
    11821 gactgggatg acgacgatca tgaccatcat catcatgacg atgatgatta tcatcaccac
    11881 gatggcggtc atcgtgacag taatggctgg caacacaacg gcgacaacat caatatcgac
    11941 gtcaacaatt tcaacagtat caccggtgag catcttactg ataagaatat ggcatggcgg
    12001 cacaatccaa actaccgtaa tggtgtgccc tatcatgatc aggatatggc aaagcggttt
    12061 catcaaaccg atgtcaacgg cggaatgagt gccacgcagc tacctgctcc aacacgcgac
    12121 agccagcgtc aggcgacagc aagtcagttt cagcaacgaa cacacgccgc ccccgtcatt
    12181 acacgagata cccaacgtca ggcagcggca cagcggttta atgaagctga acactatggg
    12241 agctatgacg acttccgcga cttcagccgt cgccaaccac tgacccagca gcaaaaggac
    12301 gccgctcgtc agcgttatca gtcggcctct cctgagcagc gccaggcagt ccgcgagaaa
    12361 atgcagacta acccgcagat ccagcagcga agggaggcag cgcgccagcg tattcagtcc
    12421 gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc agactaaccc gcagaaccag
    12481 cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa ctcccgaaca gcgccaggtg
    12541 tttaaggaaa aagtacagca gcgcccactg aaccaacagc aacgtgataa cgcccgccag
    12601 cgtatacaat cggcatcacc tgaacaacgt caggtttttc gggagaaagt tcaggagagc
    12661 cgcccacaac gtctaaacga cagtaaccat actgtcaggc tgaataacga gcaacggtca
    12721 acagtacgcg aacgtctctc tgaacgtgga gcaagaagac tggaaaggta aattgcagac
    12781 gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg gatgttccgg caaacgaaaa
    12841 attacttctt cttcgctttc gggttcgggt tcggcaggtc ggtaatgcta ccttcgaaca
    12901 cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg gtcagtgcga
    12961 tgtcttcagc atcacaaccc atttcgattg ccagcccgat ttcacccagc agctcgccgc
    13021 cgttggtacc aacaatcgca ccaccgatca cgcggtgaga ttctttgtcg aaaatcagct
    13081 tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc cacgggaagg
    13141 tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga cctacccatg
    13201 caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag tagtgtttct
    13261 taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt gccagcatcg
    13321 gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc agctgtttgt
    13381 caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg tcgaggtttt
    13441 taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc ggttcagctg
    13501 gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca acggcggtaa
    13561 ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag actttaacga
    13621 tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca atctgtgaac
    13681 ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc attaccagca
    13741 ggcgttctgg cacttctttc agttccagcg cgtcagtgga gtcccagata cgcggatctt
    13801 catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc gcattgtcga
    13861 agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc ccggtgaatt
    13921 tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc agaccaccgg
    13981 tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg gttttcggtt
    14041 cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact tttgctacgt
    14101 gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca agggtgttgt
    14161 aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca gcggagtaac
    14221 ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc atcatgacct
    14281 ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg gcgttagctt
    14341 cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc gcttgctacc
    14401 ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc accgggtcgt
    14461 tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac ggcaaccgat
    14521 ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa gccggccgtt
    14581 gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg ttgttaatga
    14641 tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag gagagagaaa
    14701 tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc atcgcggact
    14761 tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag tgggtagtac
    14821 ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc gcagtcagct
    14881 taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg atgatgcctt
    14941 tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc accgccacac
    15001 cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc gaactattga
    15061 agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca accggggtga
    15121 tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg aacgcttcca
    15181 gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc cagttacggc
    15241 tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg atttcgccaa
    15301 acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg ccagtcgccg
    15361 ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg tcttcgcgca
    15421 ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca aactcgcgtg
    15481 ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca aattcagatt
    15541 tgccttccgc tttcgcagcc ggagctgctg ccggagcttc agcttttgct gccggagccg
    15601 gcgctgccgc ttcctgtttc gcaggagctg ccgcaggtgc tgcgccttca acttcgaaga
    15661 tcataatcag cgaaccagtt ttcactttat cgccaacgtt gactttcagt tccttcacga
    15721 cgcctgcaaa cggagccgga acttccatag aagctttatc gccttctacg gtaatcagtg
    15781 actgttcagc ggcaactttg tcgcccactt tcaccatcac ttcggtcact tcaacttcgt
    15841 caccgccgat atccggaacg ttaacttctt tcacgccagc tgctggtgcg ggggtcgctg
    15901 ccggagtcgc ttcctgttta gccgccggag ctgccgcgcc tgcttcaccc gcgacttcga
    15961 agaccataat cagcgagccg gtagacactt tgtcacccac gttcactttg atctctttca
    16021 cggtacctgc aaacggagcc ggaacttcca tagaagcctt gtcgccttct acggtgatca
    16081 gcgactgttc agcttcaact ttatcgccaa ctttcaccag gatttcggtc acttcaactt
    16141 cgtcgctgcc gatatccgga acgttaacgt cttttgccgc cgcagccgcg ggtgctgcta
    16201 ccggagctgc ttctttcttc tcttctgcct gagcaggtgc agcgtctgct gcaccgtcgg
    16261 cggaatcgaa aatcataatc agtgcgccgg tctgggtttt atcgccaaca gagactttga
    16321 tctctttaac gatacccgcc tgcggagatg gaacttccat agaggctttg tcgccttcta
    16381 cggtgatcag cgactgttcg gcttcaactt tgtcgcccac tttgaccagg atctcggtga
    16441 tttcaacttc atcagccccg atgtccggta ctttgatttc gatagccatt attcttttac
    16501 ctcttacgcc agacgcgggt taactttatc tgcatcgatg ttgaatttgg cgattgcgtc
    16561 agcaaccact ttcttatcga tttcgccacg tttagccagt tcgcccagcg ccgcaaccac
    16621 gacataagaa gcatcaactt cgaagtggtg acgcaggttc tcacggctgt cggaacgacc
    16681 gaagccatca gtacccagta cgcgatagtc gtcagccggt acgtaagtac ggacctgctc
    16741 agcgaacagt ttcatatagt cggtagatgc cactgccggc gcgtcgttca tcacctgagc
    16801 gatatacgga acgcgcggag tttccagcgg atgcagcatg ttccagcgtt cacaatcctg
    16861 accatcacgc gccagctcgg tgaaggaggt cacgctataa acgtcagaac ctacgccgta
    16921 atctttcgcc aggatctctg ccgcttcacg gacgtgacgc aggatggaac cggagcccag
    16981 cagctgaact ttacctttgc taccttcaat ggtttcgagt ttgtagatac ctttacggat
    17041 accttcctca gcaccttccg gcattgccgg catgtggtag ttttcgttca gcgtagtgat
    17101 gtagtagtaa acgttctctt gtttttcacc gtacatacgc tccagaccgt catgcataat
    17161 gacagcaact tcgtaagcgt aagccgggtc gtaagagata cagttcggga tagtcagcga
    17221 ctgaatgtgg ctgtgaccat cttcgtgctg cagaccttca ccgttcaggg tggtacgacc
    17281 ggaagtaccg ccgatcagga agccacgcgc ttgctggtct ccagccgccc agcacagatc
    17341 gccgatacgc tggaagccga acatcgagta atagatgtag aatgggatca tcggcagatt
    17401 gttcgtgctg taagaggtcg ccgctgccag ccaggaacac cctgcgccca gctcgttgat
    17461 cccttcctgc agaatctgac ctttctcgtc ttctttatag taagcaacct gctcgcggtc
    17521 ctgcggggtg tactgctgac cgttcgggct gtaaatacca atctgacgga acagaccttc
    17581 cataccgaaa gtacgcgctt cgtcggcgat gatcggtacc agacgatctt tgatcgactt
    17641 gttcttcagc atcacgttca gagcacgaac gaaagcgata gtggtagaga gctctttgct
    17701 ctgctcttcc agcagcgcgc cgaagtcttg caggctcggc agctcaagct tctcggtgaa
    17761 gttcggctga cggcttggca gataaccgtg cagtttctga cgctgagcgt gcagataggt
    17821 atgctcttca gaaccttccg ggaaggtgat gtacggcagt ttttcgatat ctgcatcaga
    17881 caccggcaca ttgaaacggt cgcggatgtg acgcacgccg tccatgttca ttttcttaac
    17941 ctggtgcgcg atgtttttac cttcagccgc gtcgcccatg ccgtaacctt taatggtatg
    18001 agcaaggatt actgtcgctt tgcctttggt ttcctgcgct ttcttgaatg cagcgtagat
    18061 tttcttcgga tcgtgaccac cacggttcag tgcccagatc tgctcgtcag tccagtctgc
    18121 aaccagtgct gcggtttcag gatatttacc gaagaagtgt tcacgaacgt acgcaccatc
    18181 tttcgatttg aaggtctggt agtcgccgtc aacggtttcg ttcatcagct ggatcagttt
    18241 accgctggta tctttacgca gcagttcatc ccaacggcta ccccacatca ctttgatcac
    18301 gttccagcca gcaccttcga agatgccttc cagttcgttg atgatcttgc cgttaccggt
    18361 gaccgggccg tcaagacgct gcaggttaca gttgataacg aaaaccaggt tatccagttt
    18421 ttcacgggta gcgatggtga tcgcaccttt ggattccggt tcgtccattt caccgtcgcc
    18481 gaggaacgcg taaacggtct gtttagaggt atctttcagg ccacggtgtt ccagatattt
    18541 caggaattta gcctggtaaa tagcaccaat cggacccaga cccatagata cggtcgggaa
    18601 ctgccagaat tccggcatca gtttcgggtg cggataggaa gagaggccat tgccgtgaac
    18661 ttcctgacgg aagttatcca gctgctcctg agtcagacga ccttccagga aagcacgggc
    18721 gtaaacgccc ggggagatgt ggccctggaa gtaaaccagg tcgccgccat cctgctcgtt
    18781 gcgtgcacgg aagaagtggt taaagcacac atcataaatg gttgcggaag actggaagga
    18841 cgccatgtgg ccgcccagtt cgaggtcttt tttcgacgca cgcagcaccg tcatgatggc
    18901 gttccagcgg atagctgaac gaatacggcg ttccagttcc agattacccg gatactccgg
    18961 ttgttcttca acggggatgg tgttgatgta gttgctgata cctgtgcctg cggctacgtt
    19021 tacaccgcct ttgcgggctt cagcaagcag ttggtcgatc agatactgag cacgctcaac
    19081 accttcttca cggatgaccg attcgatcgc ctggagccag tcgcgagttt cgatcggatc
    19141 cacgtcattt gggaaacgtt ctgacatggg ttattcctta tctatctaat aacgttgagt
    19201 tttctggaac ctgtcccatt gaactctcgc cagaaagctc aataagacag gttctacgtt
    19261 tagttaccgc gcttttatat gcgcttgatt tacaacatct tctggataat ttttaccaga
    19321 aaaatcacta attctttcgt tgctccagac gacgcagaga acgctcacgg cggctctctt
    19381 cacggcttct gtcgagcaaa atttcttcga taaaggccag atggcgatgc gatgcttcgc
    19441 gcgcttcttc cggcttaccg gccataatcg cttcaaatat gcgggtgcgg tgactactca
    19501 ccagcggcag catctcgcga cgcgaataga gcaattcgaa gttctggcgg acattctggg
    19561 ccaacatcgg ctccatacac cttagcagat gaagcagaac cacattgtgg gccgcttcgg
    19621 tgacggcaat ctgatactgg agtacggcgt ttgattccgc gtccagatcg ccagactgct
    19681 gcgccagctc tatggcgtgg tggagttcac ggatgcgttc cttgtcttca tcggtactac
    19741 gcagcgcggc gtaataagcg gcgatacctt ccagggcgtg tcgtgtttcg agcaagtcat
    19801 actgtgattc aggatggtcg gagagcagct ccaccagcgg atcgctgaag ctttgccata
    19861 ggctgctctg gacaaaagtg ccgccaccct ggcgacgaag caacaagccc ttcgcttcga
    19921 gacgttgaat cgcctcacgc aaggagggac gggagacgtc gaactgtttt gccagttcgc
    19981 gttccggtgg gagtttttcg cccgggcgga gagtgccttc gaggatcaaa aactccagtt
    20041 gctgctcaat cacatcggag agttttggtt ggcggatttt gctgtaggcc atgagttcct
    20101 gtcttaagcc acttgccgaa gtcaattggt cttaccaatt tcatgtctgt gacgctaaag
    20161 taacaaagta ttcaccttat gtccatacag gttttgattg aaatcatgaa actgtgcaca
    20221 ttttaacaac ttgacatata taacgtttca aagttgtaac tatgcacaaa tgtagacttt
    20281 acgtaggaaa gggatttttt aacgattatt aaactataaa aatccgaatt gaaccgattc
    20341 acttaccaat tttttgattt ttaagtcaat aaaaacgaat ttaaattcac tctgcatatt
    20401 gagaggggtt gaggctgagc tttacaaacg gtttcttttt aagcaactca tcttcaacca
    20461 tgcataaagc gggtgcattc gctgccgcat accattattc ttgatctgac ggaagtcttt
    20521 ttgtaacaat tcaaacttcc ttgatgtaaa taaatcaata caacaaacgg aattgcaaac
    20581 ttacacacgc atcactgcgt agatcaaaaa aacaatcacc gcacgaggtt tcatgatgga
    20641 aggtcaacag cacggcgagc agctaaagcg cggccttaaa aaccgccata ttcagcttat
    20701 cgcgctgggt ggcgcgatag ggacagggct attcctgggt agcgcctccg taatacagtc
    20761 cgcagggcca gggattatcc tgggttacgc cattgctggt tttatcgcct ttctgatcat
    20821 gcgtcagctg ggtgaaatgg tggtcgaaga acctgtcgca ggctccttta gccactttgc
    20881 ttataaatac tggggcagtt ttgccggttt cgcctctggc tggaactact gggtactgta
    20941 cgttttagtt gccatggcag agctgactgc cgtgggtaaa tacattcagt tctggtatcc
    21001 ggaaatccca acctgggttt ctgccgccgt attctttgtg gtgattaacg ccatcaacct
    21061 gaccaacgtt aaagtgtttg gcgagatgga gttctggttt gccattatca aagttattgc
    21121 ggtagtagcg atgatcatct tcggcggctg gctgctgttc agtggcaacg gcggcccgca
    21181 ggcgagcgtt agcaacctgt gggatcaagg tggtttcctg ccgcacggct tcaccggact
    21241 ggtgatgatg atggcgatta tcatgttctc gttcggtggt ctggaactgg tggggatcac
    21301 cgcagcagaa gctgataacc cggagcaaag tatcccgaaa gcaactaacc aggttatcta
    21361 ccgcatcctg attttctata ttggttcgtt agccgttctg ctctcactga tgccgtggac
    21421 ccgcgttacc gccgatacca gtccgtttgt gctgatcttc cacgagttag gcgatacctt
    21481 tgtggcgaat gcgctgaaca tcgtggtact gactgcggcg ctctccgtgt acaacagctg
    21541 cgtatattgc aacagccgta tgctgtttgg tctggcacaa caggggaacg cgccaaaagc
    21601 gctggcgtct gtcgataaac gcggcgtacc ggttaacacc attctggtgt ctgcgctggt
    21661 tacagcattg tgcgtattga ttaactatct tgctccggaa tccgcatttg gcctgttaat
    21721 ggcactggtg gtatccgcac tggtgatcaa ctgggcgatg atcagtctgg cgcatatgaa
    21781 gttccgtcgc gccaagcagg aacaaggcgt ggtaactcgc ttccctgctc tgctttatcc
    21841 gctgggtaac tggatctgcc tgctgtttat ggcggcggta ctggtgatta tgctgatgac
    21901 cccaggaatg gcgatttcgg tatacctgat cccggtatgg ctgatcgtgt taggtatcgg
    21961 ctatctgttt aaagagaaaa ccgccaaagc cgtaaaagcg cattaatctc tctacgccct
    22021 cacccgtaca gggtgagggc aataatctct acaccaacgc cccgtaaatc gtcagtagcg
    22081 caatcaccac cacgaccacg aacgaggttt tcttcgccat tgaaaccgct gccttcggcg
    22141 tctccacctt atcgacatgc ggttcacgcg ccagagagaa ctgcgccaga cgcgttaata
    22201 cctgatattg cgaagtatgg aaatcaccca gcgaagcaaa ccaggccggt aacgctttct
    22261 caccatgacc gatcaaggca tataccacac ccgcaagacg aaccggcacc caatccagta
    22321 catgaagcac tgcatcaatg ccagactgta aacgatgatg cggcgtctgg tatcgcgcca
    22381 gccagtattg ccatgcacgc aagaacgcat accccatcag cgtaacgggt ccccaggttc
    22441 cccccacaat cagccagaac agcggtgcaa gataaaaacg aaagttaatc cacagcaatg
    22501 cattttgcag ctcacgcaaa tactcacgtt cgtcgcagcc tgccgggacg ccgtgaatca
    22561 tggtgagttc gccagccatc gtggcacggg catggctatc attacgtgaa gcagctgtca
    22621 gataagcatg ataatgaaga cgaactttac ctgcgccaat acacagcaaa ccaatcagca
    22681 gccacaccag cagcgtggga acgttgaaca atactccctg caatgcgcgt aacagtaaaa
    22741 aagtcacacc catcgcaata atggtcatgc ctaacgtgcg cccgagagaa aaatgtttca
    22801 cccgccgaaa gaacgcttca agacgatgat caagctgcca gtgctcgccc aacttaaaca
    22861 ggcgctcgaa aattaacacc agtaaggttg taaatagcgt catgttgtct ccttgctgac
    22921 cagcgcacga aaccgtgccc aatcaaatgc aggaccggga tcggttttcc gatccggcgc
    22981 aatatcacaa tggcccgtca tgttattagc gatatcggga tagcgatcta tcagtgcccg
    23041 cgtaaccgcc gcaagctgtt gatactgcgc atcggtatac gccagagtat cggtgccttc
    23101 aagctcaatc ccaatagaaa aatcattgca gcgttcacgc ccctgatact gagagactcc
    23161 cgcatgccag gcacgtttat cgaaaggaac atactggact atttcaccat cacggcgaat
    23221 caaacagtga gcggagacgc gcaaatgggc gatctcagca aagaaaggat gtgcctgcgg
    23281 atcaatagtt ccagtgaata atgcgtcgat ccacggaccg ccaaactcgc ctggcggcag
    23341 gctaatattg tgcaccacca gcagggtggg tgtttcgtca tccaggcggc aatcgtaatg
    23401 tggtgaggga acgcggcgcg cgccaaccag ccacccctgt tctaacaaca tgcaggatct
    23461 ccttatatgt ggtgctaata cccggttcag agtagcatgt ttctacctta tgattcgtta
    23521 gctatctgga gttttaacat gccgcctcgc cgctacaacc ctgacacccg acgtgacgag
    23581 ctgctggaac gcattaatct cgatatcccc ggcgcggtgg cccaggcgct gcgggaagat
    23641 ttaggcggaa cagtcgatgc caacaatgac attacggcaa aacttttacc ggaaaattct
    23701 cgctctcatg ccacggtgat cacccgcgag aatggcgtat tttgtggcaa acgctgggtt
    23761 gaagaggtgt ttattcaact ggcaggcgac gatgtcacca taatctggca tgtggatgac
    23821 ggcgatgtca tcaacgccaa tcaacccttg ttcgaacttg aaggcccatc ccgcgtgctg
    23881 ttaacgggcg aacgcactgc gcttaatttt gtgcaaaccc tttcaggagt tgccagtaag
    23941 gtacgccact atgtcgaatt actggaaggc accaacacgc agttgttgga tacgcgcaaa
    24001 accttacctg gcctgcgttc agctctgaaa tacgcggtac tttgcggcgg cggagcgaat
    24061 caccgtctgg ggctttctga tgccttcctg atcaaagaaa accatattat tgcctccggc
    24121 tcagtgcgcc aggcggtcga aaaagcgtcc tggctgcatc cggatgcgcc agtagaagtc
    24181 gaagtagaga atctggaaga acttgatgag gccctgaaag ccggagccga tatcatcatg
    24241 ctggataact tcgaaacaga acagatgcgc gaagccgtca aacgcaccaa cgacaaggcg
    24301 ctactggaag tgtctggcaa cgtcactgac aaaacactgc gcgaatttgc cgaaacgggc
    24361 gtggacttta tctccgtcgg tgcgctaact aaacacgtac aagcactcga cctttcaatg
    24421 cgttttcgct aattgctgac agatttatcc gctccatcgc gggcggataa tctccccttc
    24481 cccaactttc ttcgtaacgc ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa
    24541 cctcggaatc tgctttcccg tttcgttttt tgcccctcgc ctgtgctgat ggcgtttgcc
    24601 aaagtagcac caaccaaatc aaggagcgaa acagatggac aagcaacgcg gttttacact
    24661 tatcgaactg atggtggtta ttggcatcat tgccatttta agcgccattg gtattcccgc
    24721 ttatcaaaac tacctgcgca aagccgcact caccgacatg ctacaaacct ttgtgcctta
    24781 ccgtaccgcc gtagagttgt gcgcgctgga acatggtgga ttagatacct gcgacggtgg
    24841 cagcaatggc attccctcgc ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa
    24901 gggcgtggtg tcgctgaccg ggcaagaaag tctcaatggg ctaagcgtcg tcatgacgcc
    24961 aggttgggat aacgcaaacg gcgtcaccgg ctgggcgcgc aactgcaata ttcaaagtga
    25021 cagcgcattg cagcaagcct gcgaagatgt cttccgcttt gatgacgcca actaaggagc
    25081 ggcaatgaat attccacagc tcacggccct gtgcctgcgt tatcagggag tcttgctgga
    25141 tgccagcgaa gaagtggttc atgttgcggt ggtcgatgcc ccctcacatg agttgctgga
    25201 cgcattgcat ttcgctacca ccaaacgtat tgagatcacc tgctggacgc gccaacaaat
    25261 ggaaggtcac gccagtcgca cacaacagac attgcccgta gctgttcagg agaagcatca
    25321 gcccaaagca gagttgctga ctcgaacgtt acaatctgcg ctggaacaac gcgcgtctga
    25381 tattcatatc gaaccagcgg acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt
    25441 gcatccttta ccggatgttt caccggatgc cggagtcgca ttaacagcca gattaaaagt
    25501 gctgggaaac ctggatattg cggaacatcg cctgccgcag gacgggcaat tcactgtcga
    25561 actggcagga aacaccgtct catttcgtat tgcgacctta ccatgtcggg gtggtgaaaa
    25621 ggtggtatta aggttgttac agcaggtgag ccaggcactg gatgttaaca cgctgggaat
    25681 gcagccgtta caactggcgg actttgctca tgccttgcaa caaccacagg gactggtgct
    25741 ggtaactggc cctacaggca gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct
    25801 gaataccgct gacattaata tttgtagcgt cgaagatccg gttgagatcc ccatagccgg
    25861 actaaaccag acgcaaatcc atccgcgtgc cgggctcacc tttcagggtg ttttgcgtgc
    25921 gttattgcgc caggatcctg acgtcatcat gatcggagag atccgcgatg gcgaaacggc
    25981 agaaattgcc attaaagccg tgcaaaccgg tcacctggtg ttgtccacgc tacacaccaa
    26041 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gttgcccgct ggatgctatc
    26101 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    26161 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    26221 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    26281 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    26341 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    26401 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    26461 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    26521 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    26581 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    26641 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    26701 tggctctgct ggcggaacag catcccagta aacaatggca agcgttgctg caatcgctgg
    26761 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    26821 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    26881 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    26941 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    27001 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    27061 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    27121 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    27181 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    27241 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    27301 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    27361 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    27421 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    27481 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    27541 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    27601 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    27661 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    27721 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    27781 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    27841 cgggcctcgc agcggcagct tgacggtttt accttctgct gcgcggtatt ccgcaacgcc
    27901 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    27961 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    28021 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    28081 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    28141 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    28201 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    28261 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    28321 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    28381 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    28441 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    28501 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    28561 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    28621 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    28681 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    28741 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    28801 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    28861 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    28921 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    28981 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    29041 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    29101 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    29161 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    29221 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    29281 cctatgtact gtgggttgtg ccattgctga tagaaaactc actgtataaa aaagcgaatc
    29341 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    29401 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    29461 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    29521 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    29581 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    29641 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    29701 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    29761 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    29821 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggg
    29881 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    29941 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    30001 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    30061 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    30121 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    30181 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    30241 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    30301 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    30361 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    30421 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    30481 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    30541 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    30601 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    30661 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    30721 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    30781 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    30841 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    30901 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    30961 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    31021 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    31081 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    31141 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    31201 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    31261 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    31321 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    31381 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    31441 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    31501 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    31561 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    31621 ccactcggca attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    31681 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    31741 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    31801 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    31861 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    31921 cacccacggg tgttcaatgg cttcgcctag cttcataccc agtttacgca tcatgccgga
    31981 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    32041 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    32101 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    32161 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    32221 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    32281 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    32341 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    32401 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    32461 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    32521 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    32581 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    32641 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    32701 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    32761 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    32821 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    32881 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    32941 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    33001 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    33061 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    33121 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    33181 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    33241 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    33301 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    33361 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    33421 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    33481 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    33541 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    33601 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    33661 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    33721 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    33781 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    33841 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    33901 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    33961 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    34021 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    34081 aagaaagatg acggattacc gtgcgaatag catgttgatg ccagtaatca acggaatagt
    34141 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    34201 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    34261 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    34321 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    34381 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    34441 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    34501 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    34561 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    34621 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    34681 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    34741 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    34801 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    34861 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    34921 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    34981 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    35041 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    35101 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    35161 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    35221 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    35281 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    35341 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    35401 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    35461 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    35521 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    35581 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    35641 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    35701 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    35761 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    35821 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    35881 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    35941 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    36001 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    36061 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    36121 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    36181 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    36241 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    36301 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    36361 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    36421 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    36481 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    36541 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    36601 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    36661 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    36721 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    36781 tgcctgtcgc ctgaggccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    36841 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    36901 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    36961 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    37021 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    37081 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    37141 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    37201 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    37261 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    37321 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    37381 cagcataagg aattacctta gtgtggcgta atgccccacc ggtataaacg gcgatatcca
    37441 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    37501 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    37561 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    37621 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    37681 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    37741 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    37801 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    37861 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    37921 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    37981 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    38041 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    38101 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    38161 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    38221 ctgtaaaacc ggataaagtt ctacaaagcg agccaaacgt ttcatcgtat cgccccggcc
    38281 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    38341 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    38401 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    38461 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    38521 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    38581 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    38641 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    38701 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    38761 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    38821 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    38881 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    38941 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    39001 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    39061 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    39121 cctgcaaatt ggcctcttgt gacgctttca gacctgcggg gcagaaatac tgtgtctcat
    39181 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    39241 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    39301 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    39361 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    39421 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    39481 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    39541 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    39601 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    39661 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    39721 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    39781 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    39841 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    39901 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    39961 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    40021 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    40081 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    40141 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    40201 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    40261 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    40321 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    40381 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    40441 gcattcaggg tgacgcgcat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    40501 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    40561 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    40621 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    40681 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    40741 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    40801 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    40861 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    40921 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    40981 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    41041 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    41101 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    41161 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    41221 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    41281 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    41341 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    41401 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    41461 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    41521 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    41581 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    41641 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    41701 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    41761 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    41821 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    41881 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    41941 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    42001 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    42061 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    42121 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccagccgt
    42181 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    42241 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    42301 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    42361 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    42421 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    42481 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    42541 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    42601 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    42661 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    42721 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    42781 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    42841 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    42901 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    42961 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    43021 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    43081 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    43141 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    43201 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    43261 tgccccttta acagcgctac ctactaccag cacgatcatc agcaggatga tagatccgag
    43321 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    43381 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    43441 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    43501 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    43561 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca agatactgaa
    43621 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    43681 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    43741 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    43801 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    43861 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg cgccagtggg
    43921 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    43981 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    44041 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    44101 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccgcc
    44161 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    44221 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    44281 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    44341 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    44401 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    44461 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    44521 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    44581 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    44641 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    44701 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    44761 ccgggactgg cgacaatcag atctgccgcc atcaaccatt catcattcag actgcccgtg
    44821 tggcgttcta cggcttcggg taatttatcc aggctaggcg gtgtcatacg cgtatccata
    44881 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    44941 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    45001 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    45061 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    45121 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    45181 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    45241 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    45301 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    45361 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    45421 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    45481 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    45541 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    45601 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    45661 agaaatattt ccaacgagcg atcaaccctt tggtatcttt acgcaccact ttgcgataat
    45721 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    45781 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    45841 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    45901 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    45961 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    46021 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    46081 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    46141 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    46201 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    46261 gcggtcaata ccagcagctt ttgccgcctc gcccacctgt acatggcagt cttcgctttc
    46321 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    46381 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    46441 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    46501 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    46561 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    46621 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    46681 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    46741 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    46801 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    46861 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tcctggcaat
    46921 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    46981 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    47041 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    47101 cgtcagagca accacgcgcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    47161 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    47221 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    47281 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    47341 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    47401 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    47461 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    47521 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    47581 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    47641 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    47701 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    47761 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    47821 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    47881 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    47941 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    48001 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    48061 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    48121 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    48181 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    48241 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    48301 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    48361 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    48421 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    48481 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    48541 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    48601 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    48661 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    48721 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    48781 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    48841 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    48901 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    48961 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    49021 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    49081 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    49141 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    49201 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    49261 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    49321 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    49381 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    49441 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    49501 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgcta acgccagctt
    49561 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    49621 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    49681 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    49741 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    49801 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    49861 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcattgt tcagttcgcg
    49921 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    49981 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    50041 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    50101 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    50161 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    50221 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    50281 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    50341 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    50401 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    50461 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    50521 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    50581 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    50641 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    50701 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    50761 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    50821 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    50881 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    50941 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    51001 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    51061 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    51121 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    51181 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    51241 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    51301 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    51361 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    51421 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    51481 gcaatgcgtt tggcaaaacg ctcttcacca taggttttca atacccaggc gatatcggct
    51541 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    51601 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    51661 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    51721 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    51781 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    51841 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    51901 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    51961 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    52021 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    52081 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    52141 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    52201 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcgacagac gcgataattt
    52261 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    52321 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    52381 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    52441 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    52501 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    52561 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    52621 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    52681 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    52741 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    52801 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    52861 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    52921 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DADLTF010000010        50328 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.010, whole genome shotgun sequence.
ACCESSION   DADLTF010000010 DADLTF010000000
VERSION     DADLTF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.010"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..373
                     /locus_tag="JN749_000594"
     CDS             <1..373
                     /locus_tag="JN749_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367113.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            484..1407
                     /locus_tag="JN749_000595"
     CDS             484..1407
                     /locus_tag="JN749_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7367114.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JN749_000596"
     CDS             complement(1411..2229)
                     /gene="nlpA"
                     /locus_tag="JN749_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HAY7367115.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2451..2744
                     /locus_tag="JN749_000597"
     CDS             2451..2744
                     /locus_tag="JN749_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367116.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JN749_000598"
     CDS             complement(2785..3975)
                     /gene="nepI"
                     /locus_tag="JN749_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HAY7367117.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4186..4638)
                     /locus_tag="JN749_000599"
     CDS             complement(4186..4638)
                     /locus_tag="JN749_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HAY7367118.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4691..6025)
                     /locus_tag="JN749_000600"
     CDS             complement(4691..6025)
                     /locus_tag="JN749_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7367119.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6200..7966
                     /gene="adeD"
                     /locus_tag="JN749_000601"
     CDS             6200..7966
                     /gene="adeD"
                     /locus_tag="JN749_000601"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAY7367120.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPAVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JN749_000602"
     CDS             complement(8012..9403)
                     /gene="uhpT"
                     /locus_tag="JN749_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HAY7367121.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JN749_000603"
     CDS             complement(9541..10860)
                     /gene="uhpC"
                     /locus_tag="JN749_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HAY7367122.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JN749_000604"
     CDS             complement(10870..12372)
                     /gene="uhpB"
                     /locus_tag="JN749_000604"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HAY7367123.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JN749_000605"
     CDS             complement(12372..12962)
                     /gene="uhpA"
                     /locus_tag="JN749_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HAY7367124.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13037..13327)
                     /locus_tag="JN749_000606"
     CDS             complement(13037..13327)
                     /locus_tag="JN749_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase isozyme 1 small subunit"
                     /protein_id="HAY7367125.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JN749_000607"
     CDS             complement(13331..15019)
                     /gene="ilvB"
                     /locus_tag="JN749_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HAY7367126.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JN749_000608"
     CDS             complement(15125..15223)
                     /gene="ivbL"
                     /locus_tag="JN749_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HAY7367127.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15788..15877
                     /gene="tisB"
                     /locus_tag="JN749_000609"
     CDS             15788..15877
                     /gene="tisB"
                     /locus_tag="JN749_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HAY7367128.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            16157..17341
                     /gene="emrD"
                     /locus_tag="JN749_000610"
     CDS             16157..17341
                     /gene="emrD"
                     /locus_tag="JN749_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HAY7367129.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17349..17846)
                     /locus_tag="JN749_000611"
     CDS             complement(17349..17846)
                     /locus_tag="JN749_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAY7367130.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17843..18205)
                     /locus_tag="JN749_000612"
     CDS             complement(17843..18205)
                     /locus_tag="JN749_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HAY7367131.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18195..18542)
                     /locus_tag="JN749_000613"
     CDS             complement(18195..18542)
                     /locus_tag="JN749_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HAY7367132.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLTWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18651..19064
                     /locus_tag="JN749_000614"
     CDS             18651..19064
                     /locus_tag="JN749_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367133.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19111..20494)
                     /locus_tag="JN749_000615"
                     /pseudo
     CDS             complement(19111..20494)
                     /locus_tag="JN749_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(20529..22244)
                     /locus_tag="JN749_000616"
                     /pseudo
     CDS             complement(20529..22244)
                     /locus_tag="JN749_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22411..23304
                     /locus_tag="JN749_000617"
     CDS             22411..23304
                     /locus_tag="JN749_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7367134.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23301..24115)
                     /locus_tag="JN749_000618"
                     /pseudo
     CDS             complement(23301..24115)
                     /locus_tag="JN749_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inactive 6-phospho-alpha-glucosidase"
     gene            complement(24115..25731)
                     /locus_tag="JN749_000619"
                     /pseudo
     CDS             complement(24115..25731)
                     /locus_tag="JN749_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
     gene            26026..26700
                     /locus_tag="JN749_000620"
     CDS             26026..26700
                     /locus_tag="JN749_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7367135.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26739..28400)
                     /locus_tag="JN749_000621"
     CDS             complement(26739..28400)
                     /locus_tag="JN749_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HAY7367136.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28596..29024)
                     /gene="ibpB"
                     /locus_tag="JN749_000622"
     CDS             complement(28596..29024)
                     /gene="ibpB"
                     /locus_tag="JN749_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HAY7367137.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29136..29549)
                     /gene="ibpA"
                     /locus_tag="JN749_000623"
     CDS             complement(29136..29549)
                     /gene="ibpA"
                     /locus_tag="JN749_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HAY7367138.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            29854..30186
                     /locus_tag="JN749_000624"
     CDS             29854..30186
                     /locus_tag="JN749_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HAY7367139.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGSKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30188..31438)
                     /locus_tag="JN749_000625"
     CDS             complement(30188..31438)
                     /locus_tag="JN749_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HAY7367140.1"
                     /translation="MMAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRP
                     SGASFTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWH
                     YDFHHRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVM
                     HQLDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAY
                     EEGWVGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPP
                     RGVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGE
                     PRKLTHNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAV
                     VFSPDGQWLAWMEGGQLWITETDR"
     gene            31503..32521
                     /locus_tag="JN749_000626"
                     /pseudo
     CDS             31503..32521
                     /locus_tag="JN749_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
     gene            complement(32482..33819)
                     /locus_tag="JN749_000627"
     CDS             complement(32482..33819)
                     /locus_tag="JN749_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367141.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33894..35042)
                     /gene="dgoD"
                     /locus_tag="JN749_000628"
                     /pseudo
     CDS             complement(33894..35042)
                     /gene="dgoD"
                     /locus_tag="JN749_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35039..35656)
                     /locus_tag="JN749_000629"
     CDS             complement(35039..35656)
                     /locus_tag="JN749_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HAY7367142.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKVVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35640..36518)
                     /locus_tag="JN749_000630"
     CDS             complement(35640..36518)
                     /locus_tag="JN749_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HAY7367143.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLLR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(36515..37204)
                     /gene="dgoR"
                     /locus_tag="JN749_000631"
     CDS             complement(36515..37204)
                     /gene="dgoR"
                     /locus_tag="JN749_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HAY7367144.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37482..38138
                     /locus_tag="JN749_000632"
     CDS             37482..38138
                     /locus_tag="JN749_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367145.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38184..38996)
                     /gene="yidA"
                     /locus_tag="JN749_000633"
     CDS             complement(38184..38996)
                     /gene="yidA"
                     /locus_tag="JN749_000633"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAY7367146.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39110..39517)
                     /locus_tag="JN749_000634"
     CDS             complement(39110..39517)
                     /locus_tag="JN749_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HAY7367147.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(39748..42162)
                     /gene="gyrB"
                     /locus_tag="JN749_000635"
     CDS             complement(39748..42162)
                     /gene="gyrB"
                     /locus_tag="JN749_000635"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAY7367148.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42191..43264)
                     /gene="recF"
                     /locus_tag="JN749_000636"
     CDS             complement(42191..43264)
                     /gene="recF"
                     /locus_tag="JN749_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAY7367149.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPRFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43264..44364)
                     /gene="dnaN"
                     /locus_tag="JN749_000637"
     CDS             complement(43264..44364)
                     /gene="dnaN"
                     /locus_tag="JN749_000637"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAY7367150.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44369..45772)
                     /gene="dnaA"
                     /locus_tag="JN749_000638"
     CDS             complement(44369..45772)
                     /gene="dnaA"
                     /locus_tag="JN749_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAY7367151.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46069..46149
                     /locus_tag="JN749_000639"
     CDS             46069..46149
                     /locus_tag="JN749_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367152.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46379..46519
                     /gene="rpmH"
                     /locus_tag="JN749_000640"
     CDS             46379..46519
                     /gene="rpmH"
                     /locus_tag="JN749_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAY7367153.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46536..46895
                     /gene="rnpA"
                     /locus_tag="JN749_000641"
     CDS             46536..46895
                     /gene="rnpA"
                     /locus_tag="JN749_000641"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAY7367154.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCLLARGS"
     gene            46859..47116
                     /gene="yidD"
                     /locus_tag="JN749_000642"
     CDS             46859..47116
                     /gene="yidD"
                     /locus_tag="JN749_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAY7367155.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47119..48765
                     /gene="yidC"
                     /locus_tag="JN749_000643"
     CDS             47119..48765
                     /gene="yidC"
                     /locus_tag="JN749_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAY7367156.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48871..50235
                     /gene="mnmE"
                     /locus_tag="JN749_000644"
     CDS             48871..50235
                     /gene="mnmE"
                     /locus_tag="JN749_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAY7367157.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        12422 a        13534 c        12688 g        11684 t
ORIGIN      
        1 aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca ttgctatcgt
       61 gagtggtatg tgtttttacg ccagcgtact catggcgacg actccggcgg ttgagctgga
      121 attgcaaatt ctaaatgcca tcttccttgg tattctctgt ggtatcggca tgctttattt
      181 tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa atacttcacg
      241 cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct ggagctacca
      301 tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc tgctgtttat
      361 taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca aacagagcca
      421 tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta tgcagaggga
      481 aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt gttgtgggga
      541 agttcagggg tctgtgcgca atacatcatg gagcaaagcc agatgtcgtc gcagtttttg
      601 actatgacgc gtttgatatt cgccggtttg attctgctga cgctgtcatt tgttcatggc
      661 gataaaatct tttctattat taacaatcac aaagatgcca ttagtctgct gattttttcc
      721 gtggttggcg cgctaaccgt acagctcact tttttgctaa ccatcgaaaa atcgaacgca
      781 gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg gttctcactg
      841 gtacgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc gctggtcggg
      901 acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc tgccgcgtta
      961 ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc ctcaacgctg
     1021 attgcccgct atggcacgtt gcctgtcgtc ggctggagta tgctgatcgg cggtctgatt
     1081 ctattgcctt tttatgccag acaagggaca aactttgtcg ttaacggcag tttgattttg
     1141 gcgttttttt atttgatcgt cattggtacg tccctgacat ttagtctgta cctgaaagga
     1201 gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc attaagtagc
     1261 gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg gctgggaacg
     1321 ctgctgattc tgtcatcggt aattttgatt tcaatggatt cccgtcgccg cgccagaaaa
     1381 ataaatcgtc cggcgcggga tgagtgagaa ttaccagcca ggcaccgcgc caccgttaaa
     1441 gatggtttct gccgctttag cgacttcgga tgattgataa gattgcagaa attccttcac
     1501 gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg gcgaattttt
     1561 atcttcaata aatacactgt cgtgcaccgg agaaagcccg gtctgctgaa tgtaagtggt
     1621 gctgataatc gccacatcaa ctttgggatc atccagtacg cgcgggagct gcgccccctc
     1681 gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg taggtaataa
     1741 cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa gcgcacgacc
     1801 aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt gcgccacggt
     1861 tttgattttt ttggaataac ccgccatggg gaagacaaac gtatttccca ccgctaccag
     1921 tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga agacgttcgc
     1981 atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa atcctaccag
     2041 ttgaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg cgacatcttg
     2101 ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac tctggtcgca
     2161 acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat gatgtgttgt
     2221 cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta aggtcgctgc
     2281 ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg aatataaagt
     2341 ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga gcgatgaaag
     2401 caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc atgaaaccaa
     2461 cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac gccgagtcgc
     2521 cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat ttacgtaaaa
     2581 tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg ttgtcagatg
     2641 aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat aatcagagca
     2701 attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag caacaactac
     2761 tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg cagtaaccaa
     2821 caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca gaccaatatt
     2881 atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa taaccgccac
     2941 ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc acgttgacca
     3001 gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg tagcaacgat
     3061 tttatcgctt ccccacaacg tcagtaccaa cgcactcaca gccagtatta acggcgcgcc
     3121 tgctaaggcc agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa
     3181 gctggcgata ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag
     3241 gttcatatac accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa
     3301 gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt
     3361 ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag
     3421 cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa
     3481 actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag
     3541 cgccttcggc accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca
     3601 gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt
     3661 agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta
     3721 gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa
     3781 ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat
     3841 tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc
     3901 caccgagaaa acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc
     3961 aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa
     4021 cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga
     4081 gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac
     4141 ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg
     4201 aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg
     4261 catttcaggt tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat
     4321 cggcgcggaa aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct
     4381 atcatcgaca atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga
     4441 ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc
     4501 gaccatcgac tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc
     4561 ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc
     4621 gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc
     4681 tcgccagtta ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca
     4741 cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa
     4801 agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa
     4861 acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca
     4921 gcacgccaac aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg
     4981 gtgagaagaa cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc
     5041 gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga
     5101 taaatgcgcc tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga
     5161 atttaccgtt gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg
     5221 aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg
     5281 cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc
     5341 cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca
     5401 gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac
     5461 ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga
     5521 caataacacc agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga
     5581 taccaatacg taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg
     5641 tgagcaaaaa tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga
     5701 tccccatcgc ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca
     5761 gcaccacggg taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac
     5821 aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca
     5881 caaaaacaat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga
     5941 cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca
     6001 cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct
     6061 tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta
     6121 atagatattc tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata
     6181 aaatcaaaat gagaaaaata tgaataattc tattaaccat aaatttcatc acattagccg
     6241 ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat
     6301 tgataatgtc tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat
     6361 taaaggacgt tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg
     6421 gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga
     6481 atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac
     6541 cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt
     6601 tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc
     6661 cgctctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg
     6721 gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg ccgtaattaa
     6781 cgggcagaat gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca
     6841 ctgtccgggt ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg
     6901 ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat
     6961 gatccgcgaa gggtccgctg cccgcaatct caacgcactg gcaccgttga tcaacgaatt
     7021 taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga
     7081 agggcatatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt
     7141 ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt
     7201 actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt
     7261 gcagcaggta ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc
     7321 ggcgagactg gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc
     7381 cgccagcgac tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat
     7441 ccataacgaa ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg
     7501 cgatgatgtg tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg
     7561 tggtttgctc ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga
     7621 cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca
     7681 ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct
     7741 gccgttaccc attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat
     7801 tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca
     7861 gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt
     7921 tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg
     7981 cgtcatgccg ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt
     8041 tctcgcggcg gattttgcgt tcttccatta ccgccactat cgccatcagg cagatacaac
     8101 caatcgcggc gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg
     8161 gcgtcccatc ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag
     8221 caaaggtgcc tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag
     8281 cagccacacc aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag
     8341 ccagatagat atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg
     8401 ccagcgcgat gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc
     8461 acagcagcgt accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg
     8521 ctttagagag tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac
     8581 gtaccacata gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca
     8641 catactcaac aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt
     8701 cgctgacctc ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt
     8761 cgctaccgta acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa
     8821 acatgccgat gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg
     8881 cgcctgcacc gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac
     8941 gacgcggcgt ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa
     9001 agccgcttaa ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac
     9061 tggcactgaa gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt
     9121 tggtgttttt gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg
     9181 agaaacccag gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat
     9241 cgttctgcgc gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga
     9301 ccaccaggta ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa
     9361 ggtccagggt cggcttgcga acctggttta ggaaagccag catgggttac tcctgaaatg
     9421 aatacctgcg tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc
     9481 caagaaaaac ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca
     9541 tcacgcttca cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc
     9601 ggcggcgata gcgataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc
     9661 cagcggccaa ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc
     9721 cgcccctgcc gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg
     9781 gccaaagaca aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa
     9841 tggcatcagc cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat
     9901 cggcccacgg ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat
     9961 aaatccgccc agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag
    10021 cgtctcggac atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag
    10081 cacatagcaa aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat
    10141 ctctttacgc gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg
    10201 ccattcaccg accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag
    10261 aaaaatcccc acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta
    10321 atgcagcgcg ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt
    10381 gttccataat gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg
    10441 cgcacacacc ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa
    10501 cgcccatagc gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc
    10561 tatccccata aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga
    10621 cacgccgtaa gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac
    10681 gccgttagca aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag
    10741 cgcgtaaccc agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc
    10801 aatttcatgt ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat
    10861 catcgaacct tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca
    10921 ggaaatgtgt aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa
    10981 accttgttgc ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa
    11041 ccgttcatcc tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat
    11101 gttgttcagc ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc
    11161 tgattcatcg atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc
    11221 ccgcatcagt gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa
    11281 ccgtcctaac aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg
    11341 cccgctctgc ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt
    11401 acgaatagca gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg
    11461 cacgctctct tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt
    11521 ttgtagcgac tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt
    11581 cagactttgc accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact
    11641 ggcgatcagc gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg
    11701 ccaggccagc gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc
    11761 cggcaatccc aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg
    11821 ccgcccgcgc cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt
    11881 attggcgaga tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa
    11941 agtcagcaac agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa
    12001 caacgccgcc gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg
    12061 gcgatagcgc gagatcagcg ctacgggcag taacgtcagt aaactaccga tcatcaataa
    12121 cggaaaatgg gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc
    12181 cagcaatacg ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa
    12241 cggaaataac agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag
    12301 gcaaaaccat gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa
    12361 caacgtcttc atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg
    12421 cccagttttt ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt
    12481 tcggcggcaa tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca
    12541 cgtttggtca gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga
    12601 taacagccgc ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt
    12661 ttggaaagaa agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg
    12721 tgaacggaga gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg
    12781 gagatatcgg gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc
    12841 gccagcgcct cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc
    12901 agcagctgcg caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc
    12961 atggtcttgt cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg
    13021 ttcaagcctt gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg
    13081 actgattacg ctgcactttc acgacatctt ccagcttatc gatttggctt atcatctgct
    13141 ccagacgctg gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg
    13201 gcagacaaag aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg
    13261 tcattacgcc cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt
    13321 tttgcatggc ttattccccc accatttcag tattcgccgc acctggcggc accatcgggt
    13381 aaactttttc ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatta
    13441 tttcctgcaa tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga
    13501 atccggcggc aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt
    13561 gctcgtagaa cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa
    13621 tgattttgac atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca
    13681 ggctgccgtc gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa
    13741 tcgccgcagg caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg
    13801 ggcgattgag cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga
    13861 taatcgcatt gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg
    13921 gatcgcacgc tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt
    13981 gccactctgc acgcagttgc gcttccacct gcgggatcaa ttgcgccagc acgtcatcaa
    14041 catccgcctg aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat
    14101 cgacatgaat aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat
    14161 caaaacgcgc accgagcaca atcaacaaat ccgcctcttg caaaatatag ttggtactgc
    14221 gcacgccgtg catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca
    14281 gcgccattaa agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg
    14341 caggcgcatt gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca
    14401 ttgtagctgc gtcacgaatg ctttcttcgc taaaggaggg tgcagcggct ttttctgcca
    14461 ccgcgggctg agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc
    14521 acaccgggct agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga
    14581 gctcttcgat atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg
    14641 tgtccacttc ctggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc
    14701 aaatcagcgg gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac
    14761 ccggcccgct acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag
    14821 cgataaaccc cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc
    14881 ttaaggcgtc gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc
    14941 cctgctgttc caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg
    15001 ttgtgcccga acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc
    15061 ttaaacgaaa aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca
    15121 gtccctacgg cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg
    15181 gcgcagttgg tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt
    15241 ttgtgtttaa caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc
    15301 agcaagggaa aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc
    15361 ccgccggagc gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta
    15421 tgtcaacact gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga
    15481 cgtatttagt gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt
    15541 tatttataca gtaaacttct ataatatcac tgttcgcaat gtgttatgcg ggggccgcat
    15601 cgttacccgg cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg
    15661 cgtgagcaca ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac
    15721 aacagctggc aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga
    15781 gacgcgtatg agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact
    15841 gcaactgctt gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag
    15901 gggagcgggt aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc
    15961 tgacgcatct tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac
    16021 atattctgcc gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg
    16081 tgatagattc ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat
    16141 aaaagcatcc gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt
    16201 actcgtggcc gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc
    16261 gcgcgatctc aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac
    16321 ttacggtgtc tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt
    16381 gatcctcgtc ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag
    16441 tttgacggta ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt
    16501 aatggcgcgt actttgccgc gtgatttata taaacggaca cagttgcgcc atgctaacag
    16561 cctgttaaac atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct
    16621 ggatacgatg tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt
    16681 gaccttcagt atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg
    16741 cctgcttacc agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat
    16801 gctgattggc ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg
    16861 cgcggtgtta gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc
    16921 ggcatttttt ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg
    16981 gcagtcggtt atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg
    17041 cgtgatgaat gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat
    17101 gctgtttccg ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc
    17161 tggtgcgctg gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc
    17221 gatgttgccg caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt
    17281 gatcgtgctg tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta
    17341 agcgcacgtc accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga
    17401 tcacgcatca cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa
    17461 agcacgctgt ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc
    17521 tggcagtcac ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca
    17581 aaaagctcaa tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt
    17641 aaaaatttcc cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca
    17701 aattcaagat ggggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa
    17761 aaatcggcag gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca
    17821 gcatcaatga cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta
    17881 tatgcgttac agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt
    17941 cacggactac gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac
    18001 gggtgtagtg ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata
    18061 acatgcccgc ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca
    18121 gcatggtacg aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc
    18181 gtctggcttt acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc
    18241 atcaatatta agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc
    18301 atcgcttttt cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac
    18361 agacacaaca acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt
    18421 tgatcaagcc caacccccgc cgccagaaat cctagtgcgg tacggatcca cgtcagaaaa
    18481 gtacgctcat tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc
    18541 attgttgctc ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt
    18601 cgtccgcccg tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca
    18661 tagcgcaaaa taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg
    18721 ggattatcca cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg
    18781 tggcggacaa aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa
    18841 ttaccactgt ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta
    18901 tcgcactggc cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc
    18961 gctggggcat tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga
    19021 tattcctgat tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc
    19081 ccgccgggtg atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt
    19141 cactccttgc gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg
    19201 tgggcgtggg cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag
    19261 actccattgg taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc
    19321 atgcattttg ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt
    19381 tgggtcatta cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa
    19441 gtcatccgtc acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc
    19501 gtagcggtta aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc
    19561 cggcagaatc tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat
    19621 atgactgact ggcgtatcga cctgtcgccg ctcctttact gatcagctta tgtgcgccca
    19681 tcatttcacc gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta
    19741 aggcattgat gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg
    19801 gatggtgata aagcccgtca tcaccgactg gcgatggcat cgcctgcgcc cataagcggt
    19861 gatgttccgg tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat
    19921 cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgattcggc tcatcatacg
    19981 aaaccaccat caggaagggt tcctcggcgc gcacgggctg ttgcagaaaa tctaccgctc
    20041 gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt
    20101 cgacgctgtt taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat
    20161 agttcgcccc atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga
    20221 aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct
    20281 taaagtagcg ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc
    20341 cggactggtt agcgtagata ccggtgaata gtccggcgcg tgcaggcgta caaaccggtg
    20401 aacaagtgta ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg
    20461 tattcagcgg tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt
    20521 ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga
    20581 tgccgccagc tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc
    20641 atcgctaacc agcgcgtacc gtagccgccg aattcagcca gcccgacata gacgccaatc
    20701 atcgcgaaca ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc
    20761 gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcggtt tgataaaacc gatcaccagc
    20821 atcacgacca cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac
    20881 ttcaccagat agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc
    20941 gccagcgccg ggatgcgcgg gaagaaaaag cccataatga tgatggtaac cagcggcacg
    21001 ttgtaaatgc cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttggcgatc
    21061 cacggcgcta ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg
    21121 accagctgct gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat
    21181 aatgtactgg cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc
    21241 gcgccgaaga aacccaccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc
    21301 atgtcggctt tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt
    21361 accagcgggt cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc
    21421 aggctttttg acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg
    21481 ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg
    21541 actgagttta atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg
    21601 cccttgccca tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca
    21661 ataccgttga tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa
    21721 attcccgcca gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt
    21781 agcgattccc cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc
    21841 ggcagaaagc agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt
    21901 ttatcataac gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc
    21961 ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc
    22021 accgacatgc cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg
    22081 ttggttaaca ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa
    22141 tagccttgtt gcgatccggt atctgttttg cgaaccttcc actaggtgat caccgccacc
    22201 agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt
    22261 atgtttatgt cccggacgca tgaatatagg gagaggtaaa atgtgtcggg tcaggcagtc
    22321 agattcttaa aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt
    22381 gtgagcataa ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta
    22441 aaaaacgaaa ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga
    22501 atttctctga tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg
    22561 cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga
    22621 tgtgggaaaa ttcttttaaa gaatggcgaa caaataactc tgcatggcaa ctgtattata
    22681 tttttaaagc caatggatat tcactcttat cactgtgaag gtttagtctt ggaacagtac
    22741 tggatggaat ttacccccac cagtatgatg gatattcccg ttggtcagca aagcgttatt
    22801 tataatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca
    22861 gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt
    22921 tgtcttatgt ctgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt
    22981 gccactttac atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc
    23041 ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa
    23101 gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac
    23161 tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc
    23221 tttaaacata aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca
    23281 acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc
    23341 aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc
    23401 gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc
    23461 gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt
    23521 tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca
    23581 tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca
    23641 gacgacgcgt ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt
    23701 ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc
    23761 cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgccgtggga
    23821 ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc ataacaaaat
    23881 caacgtcgct gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt
    23941 tttctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat
    24001 cataaaattt cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga
    24061 tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac
    24121 tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg
    24181 ttcacgcaat tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa
    24241 gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg
    24301 cagtgcaata cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc
    24361 cagggcttgc aggataccgg cagcctgatc cagctctttt tttggctctg cagcggtggt
    24421 ttagcctcgc gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg
    24481 tcccggcgtg cacatgttga actgcagaat cagggggcgg aaaaccacga agtacagcag
    24541 ggtaaagcac aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat
    24601 cgggatccag ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac
    24661 accaaagaga tacattacgg tcgacattga ggccgccagc acggcgtgta ccgcaaacag
    24721 caacggtgaa atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc
    24781 ggttaaggtt gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt
    24841 gaagtacatc gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc
    24901 aaaaccgcct tccgggaaca acgatttcag cggctcggcg ctcagactga actcttgcag
    24961 atgctgcgcc cagtacatct gaatgccgcc ttcaacagct gccggaccaa agataaacgg
    25021 tccgtagatg aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac
    25081 ccacacccca agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccatttg
    25141 tacttttggc cagccgagca atgtcagcca ggcacagggg atcatcacca ggaaggcgat
    25201 aatcaccaca taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa
    25261 cagacggtta tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt
    25321 atcgagggtt ttaatcccgg ccatcattgt cagaccgcta cctgccaccg cgtcctgagt
    25381 gaaatcgacg ccgaagtagc ttccccaggt cattcccatc gcattgatga aatagttcca
    25441 ggtcaggaaa ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc
    25501 aatgggtaaa ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc
    25561 ttcaataatg tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac
    25621 aaacatcggg ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag
    25681 cagcactggc gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc
    25741 ctcttttctc aattctgtaa gtaggaagcc tgattggtta tgcggcagac ctggagcgag
    25801 gataggaaga tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg
    25861 cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag
    25921 gtacacagca tcgaacacat ctttaaaaaa aagatttttt tcaatcgatt aagcagaact
    25981 tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagagc tagagatgat ctacaaaagc
    26041 attgcggagc ggttaagaat tcgacttaat tccgcggatt tcacgctaaa cggccttctt
    26101 cccggtgaaa aaaagctggc ggaagagttt gcggtatcgc ggatgactat ccgtaaagcc
    26161 attgacctgc tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg cacttacctg
    26221 gtgcgcaaag atgtgctgca tcaaaccgcc agcctgaccg gactggtgga ggtgttaaaa
    26281 cggcagggaa aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg
    26341 gccattgcca gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt
    26401 cgttttgtgg aagggaaacc gctaatgctg gaagacagct atatgccggt aaaactgttc
    26461 cgtaaccttt cactgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc
    26521 gggattttga ttggcggtaa ttatgaaagc ctgatgccgg tgctcgccga tagactgctg
    26581 gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat
    26641 agcgagagcg gggagttttt gaattattca gtgatgttca gaaatgccag cgaatactag
    26701 gtggagtacc atttacggcg actccacccg gaaaagagtt aaccgatact ccagaagagc
    26761 accgccagta attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg
    26821 taagagagcg ccgccgcacc actggttgga tgaagattat tagcaaacgc cagcgccgga
    26881 ggatcggtca tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac
    26941 atccgcgcca gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca
    27001 atccagctta gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga
    27061 ccaacgaccg agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc
    27121 ggcataaacc agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc
    27181 agcggaccgc ccgccagccc cagtttcaac gcggccggga atcctggcac aaagacggga
    27241 atagaaccta acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt
    27301 tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc
    27361 cccaccaggt tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg
    27421 accccggcac ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg
    27481 cgaatacgtt ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca
    27541 gtgcctttcg tagacagcga ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt
    27601 aaatccgctg gctgccccac cagatgcagc aaatcgccca gttggataat ggtatctggc
    27661 gaaggtactt ttagggtttc ttctcgtttc agacgcgagc agataatttt gtcgccgttg
    27721 agaatcggta catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg
    27781 atagtcttga tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc
    27841 tcgacattga cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg
    27901 ccgaatggat acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta
    27961 cccaggtcgc gcaagatctg ctgccctgcc cccagcgctg gcgtattggt taccgcaccg
    28021 gagaaaatcc ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta
    28081 accagaccac cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg
    28141 cgcaatgagg caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc
    28201 aggccaaatt cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa
    28261 acaaaatggc cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg
    28321 cgaaatttga cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc
    28381 gttaatgcta tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat
    28441 tatggctgaa tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt
    28501 gccgaaatat cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag
    28561 ccccaccaga atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg
    28621 ctgatagcga tacgctgcgc tgcgatgggt tctggctcat tacgaattaa atcaatatgc
    28681 agtaaaccgt ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaaactc
    28741 aggctaaatg gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc
    28801 tccggcgtgc cttttacgct caggcgcgta ccttccagtt gaatctctaa atcttcctga
    28861 cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg
    28921 tacggcggga agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa
    28981 ccgatccatt gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct
    29041 aagaagcgag taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat
    29101 ggagatgtca ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt
    29161 tttttcgctt ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta
    29221 gccccacgaa catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg
    29281 atgccctggt acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc
    29341 agcagattat cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata
    29401 gcaatgcggt aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta
    29461 ctctggctct ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg
    29521 taaagcgggg ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt
    29581 aagaccttcc acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg
    29641 actacgatgt aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt
    29701 tgcagtttat ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag
    29761 ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca
    29821 ggccaacacc cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc
    29881 atgatatgca gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg
    29941 gggaaagaag gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa
    30001 accaactggg gctctaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac
    30061 acgcttttac tgccgtggga tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa
    30121 aaaagcgaag ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat
    30181 aactgattta gcgatcggtt tcggtgatcc acagctggcc accttccatc cacgccagcc
    30241 attgaccatc cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg
    30301 ttaaatactc aacctcgccg ctttgcgcat gggcacaaac aattcgatta tccagcacaa
    30361 agcccaacca ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat
    30421 gggttaactt gcgcggttcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt
    30481 tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc
    30541 gcgggacgtt gactaacccc ggataagccc gatgatgggt aaaggttaaa cgtcgctgca
    30601 cgacgccacg cggtggcgcg ggtagggttg tttccgttcc acttaacggc gcatcacctg
    30661 ccgatttcca gctagcttca tcttgcggta actcaacgat aaatagctcc ggcactttct
    30721 cgccctttgg cgaaagtgtg tcgccaataa acgccagcgc gtgatttcct acccatcctt
    30781 cttcataagc acgattgatt tcatcactgc caggctgcgg cgtgggcgtg gttttactca
    30841 ccagcacaca ccagtggcta ccgctgtatt cacgcggatg ttgcttttgt acgttgaccg
    30901 ggccaaacgg tgcagcaacc ccgacgtttc gcaaatccag cgccggatcg agttgatgca
    30961 ttacatggtc gttataggta aagctccccc tttcaccgtt cgggctaaag acatgcacat
    31021 ggctgccacc gcgcagcgct cctggcgtat acggcgcggt aatatccatt gcatcgagat
    31081 tgctcacctt gccgccttca gcaatgactc cgcgtcgatg atggaagtcg taatgccatg
    31141 tttcatcagg attttcaggg ccgtgaataa agacatattt ctctgacttc ggatgaacgg
    31201 tcaccacgcc gacgtgtgcg ccctgtgacg cgcgatagat aacctcgacc tcgcctgtat
    31261 ggatattcac acgctcaatg gtctcgccgg taaacgacgc cccagaagga cgcacgtcaa
    31321 ataccagcca ctggctgtcg ggcgtccagg tattggtatt ggtgagcagg tgattacggg
    31381 gagcaaaggt tatctgtttc atggcgcggt cctgatacaa aacggttccc gccatcatac
    31441 ttcacaagga cttcactttc agacaatagt cttttgccat cgtgatgaat gtgagtgaaa
    31501 agatggaaca tttcgacgtg gcgattattg gcctcggccc ggcagggtcg gcgttggcac
    31561 gaaagttagc cggcaaaatg caggtgatcg cgctggataa aaagcaccag tgcggtactg
    31621 aaggtttcag caaaccttgc agattttcag cgtcaaaact gtcgacgtcg ccgcatcgct
    31681 cacgcgtaac taccagcgaa gctatatcaa tattaatcgc catgctttcg acttgtggat
    31741 gaaatcgctg atccccgcca gcgttgaggt ttatcacgat agcctgtgcc gaaaaatctg
    31801 gcgtgaggat gataaatggc atgtcatttt tcgtgcagac ggctgggagc aacatattac
    31861 cgcccgctat ctggtcggtg ccgatggcgc aaactcgatg gtgcggcgac atctctaccc
    31921 ggatcatcaa atccgtaaat atgtcgctat ccagcagtgg ttcgcagaga aacatccggt
    31981 gccgttctac tcctgcatct ttgataatgc gataactgac tgttactcat ggagtatcag
    32041 caaagatggt tattttatct ttggcggtgc ctatccaatg aaagacggtc agacgcgttt
    32101 cacgacgctg aaagagaaaa tgagcgcctt tcagttccag tttggcaaag cggtgaaaag
    32161 tgagaaatgc acggtgttgt ttccctcacg ctggcaggat tttgtctgtg gtaaggacaa
    32221 cgcctttctg attggtgaag cggcgggatt tatcagcgcc agctcgctgg aggggattag
    32281 ctatgcgctg gatagcgcag agattctgcg tgcggtgttc ctgaaacagc cggagaagag
    32341 caacgccgct tactggcgcg ccacacgcaa actgcgttta aaactcttcg gcaagatagt
    32401 aaaaagccga tgcctgaccg caccggcttt aagaaagtgg attatgcgca gtggtgtggc
    32461 gcatattcca cagttgaaag attatccaac gcgcttcaca tcacccacca gcaggatgta
    32521 agagagcgcg ccaatcaacg cgacggcgga gatataaacc agtgcaggcg cgaaaccgta
    32581 accctgcgcc aggtacccca ccaccagcgg aacggtgatg ccgcccagac caccggcgaa
    32641 gttaaacacg ccgccggtta aaccaatcag gcgcatcggt gccagagaag agaccagcga
    32701 ccaggtaatc gaagcaaaac cgttgccgaa gaatgccagc gccatcaggc acataatcat
    32761 catcggatcg ttggtgtagt tagcgcccat aatgcaggtg gagatcagca agccgcagat
    32821 aatcggcgtt ttacgcgcaa agcccagtga aaagccctta cgtaccagca gatccgcgac
    32881 ccagccagag agcaggacgc cgacaaacgc cgcgaggaat ggcaccgtgg tcataaagcc
    32941 tgctttcagc gccgtgattc ctttttcctg ggttaaatag ttcgggaacc aggttaagaa
    33001 aaaccacagt gcagaagcca ccgcaaattg cccaagataa acgccgatca gtttacgatg
    33061 gaacaccagt ttccagtctt tggctgttaa cggctgacgc gcctctttct tcaccggcgc
    33121 atcaccatcc accagaccgc cgccatcacg aatgtaatcc agttcagctt tgctgatacc
    33181 tttggtcagg cgcggcggct gataaacctt aaaccaaatc agcgaccaga taatgccgat
    33241 accaccagtg acaatgaaca cccagtgcca gctcaacatc tcctgaatcc agatcagcag
    33301 cggagtcaaa aacgccagac cgacaaactg accagacgta taaaaaccaa cggcggaagc
    33361 gcgttcatgt tccggaaacc agctggtcac catccggtta ttggtcggga aagcgggcgc
    33421 ttcgaaaata ccggttatcg cacgcagacc aattaatgac attaatcccg tggcaaagcc
    33481 ctggaataaa gtcgccactg accaaccaaa tatcgcaata aaataagtca cgcgagaacc
    33541 tacgcgatct aaaaaccaac cgccggggat ctggcatagc gtataaagcc aggcgaaggc
    33601 cgaaaataca tagcccattt ccgctttcgt aatgccgaac tcttcctgaa tatgggcgga
    33661 agccacggcc aggttagcgc ggtcaacata acaaatgacc accgtaataa agatcatcac
    33721 cagcgtcaga taacgccgac gccccggctt tgctgcatta acgggaatat ccatagcgag
    33781 ctttctccag attttgggca tagcgaagcc gctcaccatg ccctgtaatt tacagagggt
    33841 tatatttttg tattgctgtt ttagtgcccg atgaggggct tacgtggcag gaattaccac
    33901 tctgctacgc tgttatcttc atgacgccag agcggattac gccagtccgg ggcattttta
    33961 ctgaactcaa taactttagc ttcgtcgatt tccacaccta agcccggttt cgttaacggt
    34021 ttaaagaagc cgccaaccat gctgaagtct tctttgtttt tcacaaagtc gagtaactcc
    34081 gcgcctttgt tgtaatgaat gcccatactt tgttactgaa gtaccgcgtt ataggaaaca
    34141 aagtcgatat gcaggcaagc cgccagtgca atcggtccga acggacagtg cggcgcaagg
    34201 gtcacatcat aggcttctgc cattccagca attttgtagc attcggtaat accgcctgca
    34261 tgggagagat ccggttgcag aatcgaaata ccgcctgcct ccagcacgcg tttaaaatcg
    34321 aaacgtgaga acatgcgctc acccgccgcc agtggaatat gcgtttgtgc cgccaatttc
    34381 gggtagtatt cggcttgttc cgccagcacc ggctcttcaa taaacagcgg gcgatacggc
    34441 tccagttctt taatcagcac tttcgccatt ggcgcgctga cgcgaccgtg gaaatcaaga
    34501 ccaaactcaa tctgattgcc aaaagcttca cgaatttgtg ccacggtgtt gactgccgca
    34561 tctaccgcgc gggagttatc aattagcccc agttcttcac aaccgttcag tttgaaggta
    34621 tcgaagccga tttcgcgcag ggttttaatg ccgtcgataa catccgccgg acgatcgccg
    34681 ccgacccaac tgtaggcttt aattttgtcg cgaaccaggc cgcccatcag ttgccagact
    34741 ggcgcattca gcactttgcc tttgatatcc cataacgcct ggtcgatccc ggcgatggcg
    34801 ctcatcagga ttggaccgcc acgataaaat ccggcgcgat acatcacttg ccataagtcg
    34861 ttgatgcgcg aaggatcctg accaatcaaa tagtcaccca gctcgtgaac tgccgcttcc
    34921 accgtacggg cgcggccttc aatcacgggc tcgccccaac cgaccacgcc ttcatcggtt
    34981 tcaattttca ggaacatcca gcggggaggt aaacgatacg tggtaatttt ggtgattttc
    35041 attgcactgc ctctcgatac gccttaacaa atgctgctgc ctgctgcgcg gtgcgttcta
    35101 cggactgccc ggcgcgataa agatcgctgc ctaagcccgc cccaacacaa cctgcgtcta
    35161 tccactgcgc caggttttct ggcgtcacgc cgccaacggc aaagactgcg atgtccggtg
    35221 gtaataccac ttttaacgct ttgatgtatt gcggaccaaa agccgatgac ggaaatattt
    35281 tcagcgcctg cgcgcccgct tcgagcgcgg taaaggcttc ggtcgccgtc gcgcagccgg
    35341 ggcaaacggt catgccgtag cccaccgcgc ggcggatcac ttcactatgg atattgggcg
    35401 taacgatgag ctgacagccc atcctggcga gcgcatcgac ctgttcaggt ttcagtaccg
    35461 tacctgcgcc aatcaacgcc ttgtcgccat acgcatcaac gatggcggga atgctctgct
    35521 cccattgtgg ggaattcagc gggatttcaa ccgcgtcgaa cccggcgtca atcaccgcgc
    35581 caacatgcgc cagcgcctcg tcgggcgtaa tgccgcgcaa aatggcgatc agcgggagtt
    35641 tagtttgcca ctgcatgagc gatgctcctt ataccagcct gaaatgccgt gtcgcccgcc
    35701 accgccgcca cgtcgcaacc catcgcctga aaggcttgct ggtagcgcgc ggtcagcgat
    35761 gttccggcga caagggtgat ggcgtgttga tgggtcacat agtcgcgcat actggcaacc
    35821 tctgcgccaa tcaacaaacc agagagaaat tcgctgacct gttcgcggag aagtgttccc
    35881 agcacatgcg aggcgcgaac ttcaaaaagc tgcggcaata tggcgggcgc attaaggccg
    35941 cgctcaaggc cagccgcgaa ggcatcggca gagttttcct gcggcggcaa acctgcgcca
    36001 atcaatgagt gatttaacag taaatgatgt aattcaccgg tcatcacggt gcgaaaatcg
    36061 ttgatttgct ggctatcggc ctgcacccat ttgcaatgcg taccgggcat aacataaaga
    36121 gaggaaggag ccagagctcg cgcgccgatc aattgtgttt cttcgccgcg catcacattg
    36181 tggttatcgt catgagagac gcataatccg ggaataatcc agatattgtc gccaacagac
    36241 gttaattgtt cgccaataga cgaaaaacgg gcaggaacag ataaatacgg agcaactttc
    36301 cagccgacat tgctgccgac cattcctgcc atcaccactg gcgtattttc ttcacgccag
    36361 tcggtcgtga cttctgctaa caccgcagcc ggagattttc cgttcaggcg cgtgacgcct
    36421 gcttctgatt gcctgctctc caggcagtgg tcgccctgat aaagccaggc gcgcagattg
    36481 gtcgatcccc agtcaattgc gatgtagcga gctgtcatgt gatttccttt aaccttcgtg
    36541 tcgagctggc aatcatggta agcgccgcct gctctgccgc atcgccgtcc tgatgccgta
    36601 tcgcatcgaa cagcgcctta tgttcctgga gcgtttgcgg catgttggcc tcatcgccca
    36661 tccaggttcg ttcaaaaacc gcccgctgca gcgaactgat cgcaatgcta agttgctgta
    36721 acaccgggtt atgcaccgac tgcagcaccg cctcgtggta gcgaatatcc gcttcgttaa
    36781 acgcttcgcg gtcctgattg ttggcaatca tctcgttcag cgccgattca atctgcgcca
    36841 gatcgctgga ggtcgcgcgc tctgctgccc aacgggcaat cgccggttcc accagatttc
    36901 gcacttcgct catggcactg ataagccgtg ggtcgtagtc gttttccagc acccattgca
    36961 gtacgtcagt gtcgaggtaa ttccactggt tacgcggtgc cacaaacgcc ccgcgataac
    37021 gtttcatttc aatcagccgc ttcgccatca gcgaacggaa cacctcacgg atgatgttgc
    37081 gcgaggttgc aaactcttca cagagctccg cctcagccgg aagtggcgag ccaggtacgt
    37141 atttgccgtg aacgatctgt ttacccagcg taatgacaat gcgatcggtt ttattgagag
    37201 tcatggagag tccttgtgct ctgtatgttc ttctctactt taccccgatc gatgcataac
    37261 gcggcaactt tgtagtacca gcgtgatgac gttcgcgttt gccgtgcgtg taatgtagta
    37321 caaacttata ttgttgtgct acaatttaga tcaaaaaaag aacaatgtat aaaaaaatga
    37381 catgcgtcgg gcagaaatct gaaaagggat atcaggcgct aaacaggagg gaaagaagag
    37441 tatgctttca acggcttagc tactcgttta aaagattaat catgaagttg aattttaagg
    37501 gattttttaa ggctgccggt ttattcccac tggcgctgat gctttcaggc tgtatctcgt
    37561 atgctctggt ttcccatacc gcaaagggta gttcaggaaa gtatcaatcg cagtcagaca
    37621 ccatcactgg gctatcgcag gcaaaagata gtaatggaac aaaaggctat gtttttgtag
    37681 gggaatcgct ggattacctt atcactgatg gtgccgatga catcgttaag atgctcaatg
    37741 atccagcact taaccggcac aatattcagg ttgccgatga cgcaagattt gttttaaatg
    37801 cggggaaaaa gaaatttacc ggcacaatat cgctttacta ctactggaat aacgaagaag
    37861 aaaaggcact ggcaacgcat tatggttttg cctgtggtgt tcaacactgt accaggtcac
    37921 tggaaaacct aaaaggcaca atccatgaga aaaataaaaa catggattac tcaaaggtga
    37981 tggcgttcta ccatccattt aaagtgcgat tttatgaata ctattcaccc agaggcattc
    38041 cggatggtgt ttccgcagca ttactgccag tgactgttac gctggacatc attactgcac
    38101 cgctgcaatt tctggttgta tatgcagtaa accaataatc agtaagcggg caaacgcgtt
    38161 tatgctgttt gcccgcccac agattaattc agcacatact tctcaatagc aaacgccacg
    38221 ccatcttcaa ggttggattt tgtgacaaag ttcgccattt ctttcaccga aggaatagcg
    38281 ttatccatcg ccacaccgac gcctgcatat tcaatcattg cgatgtcgtt ttcctgatcg
    38341 ccaatcgcca tgatttcttc cggtttaata cctaacacgt cggccagtga tttcacaccc
    38401 gtacctttgt taacgcgttt atcgaggatt tcgaggaagt acggcgcact tttcagcacg
    38461 gtatatttct ctttcacttc ctgcggaata cgcgcgatag cctggtcgag gatggcgggt
    38521 tcatcaatca tcatcacttt caggaactgg gtattggggt ccattttctc cgcttcgcag
    38581 aacaccagcg gaattgtggc aacgaaggat tcatgcaccg tgtagtagct gatatcgcgg
    38641 ttagcggtgt acagcgtggt gcggtccagg gcgtggaaat gagaaccgac ttcgcgagag
    38701 agtttttcca ggaaacgata gtcgtcatag ctgagagcgg tttgcgccac ggtgctacca
    38761 tcagcggcct tctgtaccag cgcgccgtta taagtaatgc agtagtcgcc cggctgttcc
    38821 atatgcagct ctttcaggta gttgtgcaca cctgcatacg gacgacccgt cgttagcacg
    38881 acattcacgc cacgggcgcg agctgcggca atcgcatttt taacggcggg tgaaatggtg
    38941 tgatcgggca gcagaagggt gccatccata tcgatagcaa tgagtttgat agccatgagt
    39001 tcccaaggta gattagttcc tgacccatgc taacgcgatt ccgctcaaaa atcagtacaa
    39061 cacccgaggg aaaaggggat gcaacgcggg ttgcatgctc cccatttgct tagcggaaga
    39121 gtttcccttt cagcagttcc atgcctgcgg aaagcagatc gttattggct tgtgctgaca
    39181 cttcaccttg cggtgagagc gcatcaataa tcttcggcaa ttgctctgcc agtaaactgg
    39241 aagccgtact ggtatccacg ccaagttttt gcccgagatc ggacaccgca tttgtgccga
    39301 gcgccgattc cagttgctcg ccgctaactg attgatttct ctgttgatta ctcaaccaag
    39361 ttgagagaat ggcccctaag ccgccacttt gcagtttttc cagcagcacc tgaatgccac
    39421 cctgctcctc aacccaactt aaaatcgcct gatatttccc cgcatcgcct ttcagaaagg
    39481 caccgacaac ttcatcaaaa agccccatga taatcacctg taaagcgtta cgtgttgacc
    39541 caaaaagtat agatctgcgg gatgataatt gcggattgca gaaataaaaa gggcggagat
    39601 gatctccgcc cttttcttat agcttcttgc cggatgcggc gtgaacgcct tatccggcct
    39661 acaaaatcat gcaaaatcaa tatattgcag gagtctcgta ggactaataa gcgtagcgca
    39721 tcaggcacgc tcgcatggtt aacgccatta aatatcaata ttcgccgctt tcagggcgtt
    39781 ctcttcaata aacgcacgac gcggttctac ggcgtcgccc atcagcgtgg tgaacaactg
    39841 gtcagcggca atcgcatcct taacggtaac gcgcagcata cgacggcttt ccgggtccat
    39901 ggtggtttcc cacagctgtt ccgggttcat ctcgcccaga cctttataac gctggatgga
    39961 gaggccacga cgggactctt tcaccagcca gtccagcgcc tgctcgaagc tggctaccgg
    40021 ctgacgacgc tcgccacgtt cgataaacgc atcttcttcc agcaagccac gcagtttctc
    40081 acccagcgtg cagatacgac gatattcgcc accggtgata aactcgtgat ccagcggata
    40141 gtcagtatcc acaccgtggg tacgcacgcg aacaatcggc tcgaacaggt tttgttcggc
    40201 attggtgtgg acatcaaact tccactggct gccgtgctgt tctttgtcgt tcagttcgct
    40261 gaccagcgcg ttcacccagc gggtaacggt ctgctcatca gaaaggtcag cttccgtcaa
    40321 cgtcggctga tagataagct ctttcagcat tgctttcgga taacgacgct ccatgcggtt
    40381 gatcattttc tgcgtcgcgt tgtactcaga taccagtttc tctaacgctt cgccagccaa
    40441 tgccggtgca ctggcgttgg tgtgcagcgt tgcgccgtcc agcgcgatag agatctggta
    40501 ctgatccatc gcttcgtcgt ctttaatgta ctgttcctgc ttgcctttct tcactttgta
    40561 cagcggcggc tgagcgatgt agacgtgacc gcgttcaacg atttccggca tctgacgata
    40621 gaagaaggtc aacagcagcg tacgaatgtg cgagccgtcg acgtccgcat cggtcatgat
    40681 gatgatgctg tgataacgca gtttgtccgg gttgtactcg tcacgaccga taccacagcc
    40741 aagcgcggtg ataagcgtcg ccacttcctg agaagagagc atcttatcga agcgcgcttt
    40801 ctcgacgtta aggattttac ccttcagcgg cagaatcgcc tggttcttgc ggttacgccc
    40861 ctgcttcgca gagccgcccg cggagtcccc ttccaccagg tacagttcgg aaagcgccgg
    40921 atcgcgttcc tggcagtctg ccagtttgcc cggcaggcca gctaagtcga gcgcaccttt
    40981 acggcgggtc atttcacgcg cgcgacgcgc cgcttcacgg gcacgggcag catcgataat
    41041 tttgccaacc acgattttcg catcagttgg gttttccagc aggtattccg ccagcagttc
    41101 gttcatctgc tgttcaaccg ccgatttcac ctcagaagaa accagtttgt ctttggtctg
    41161 ggaggagaat ttcgggtccg gcacttttac ggaaacgacc gcaatcaggc cttcacgcgc
    41221 atcgtcaccg gtggcgctga ctttggcttt tttgctgtag ccttctttgt ccatgtaggc
    41281 attcagggta cgggtcatcg ccgcacggaa gcctgccagg tgagtaccgc cgtcacgctg
    41341 cggaatgttg ttggtaaagc agtagatgtt ttcctggaag ccatcgttcc actgcagcgc
    41401 cacttcaacg ccaataccgt ctttttcggt ggagaagtag aagatatttg ggtggatcgg
    41461 cgttttgttc ttgttcagat attcaacgaa cgctttgatg ccgccttcat agtggaagtg
    41521 gtcttctttg ccgtcacgct tgtcgcgcag acgaatggaa acgccggagt tgaggaacga
    41581 caactcacgc agacgtttcg ccagaatttc atattcgaac tcggtcacat tggtgaaggt
    41641 ttcgaggctg ggccagaaac gcaccatggt gccggttttt tcagtctcgc cggtaaccgc
    41701 cagcggagcc tgcggtacac cgtgttcgta gatctgacgg tgaattttac cctcgcgctg
    41761 aataaccagc tccagttttt gcgacagggc gtttactacc gaaacaccaa cgccgtgcag
    41821 accgccggac actttatagg agttatcgtc aaatttaccg cctgcgtgca gaacggtcat
    41881 gatcacttcc gccgccgata cgccctcttc cgggtgaata ccggtcggaa tgccgcgccc
    41941 gtcatcctgt acagagacag agttatcggc gtgaatggtg acgataattt ctttacagtg
    42001 acccgcgagc gcttcgtcga tagcgttatc taccacctcg aataccatgt ggtgcagacc
    42061 ggtgccgtca tccgtgtcgc cgatatacat acccgggcgc ttacgcaccg catccagccc
    42121 tttcaggact ttgatactgg aggagtcata agaattcgac atcaacgttt ctcgctcatt
    42181 tatacttggg ttaatccgtt attttaccct tttccacggt aaacatcttc gaattttcgt
    42241 ccgacatgtc tataacgtgt tcagcactga tcgcgctgac aaagacctgt gattgcgtcg
    42301 cttttaagcg gctggcaagc aacccgcgac gctcatcatc aagctcagag gcaaaatcat
    42361 ctatcaggta gagacaccgc cgcccgcttt cacgagtgag gaactctcct tgcgccagac
    42421 gtaaggcgca catcaacagc ttaagctgcc cacgcgataa ggtatcttcc accggcgcac
    42481 cgtcggcgcg aatgcgtaag tccgctttat gcgggccatg cgcggtgtag gttagctggc
    42541 gatcgcgttc aaaattacgt tccagcacct cagcgtactc tgtctctttc tcccagccgc
    42601 gctggaaaga gaaagtcaga gaaaactcag ggagaaattg cttacaggta tcatccatat
    42661 cggccgcgat accggcgcta tactccgcgc gccaggtgct gatttgctcc gccagcggga
    42721 tcagttcttt atcccacggg cgtagctgtt cgtaacgagt cacctggcgc agcgccgcat
    42781 tgcgctgctt gagcaatcgc ttgagattgc tccaggcggt gaaaaatcta ggttcgttgt
    42841 gaaagcatcc ccagtcgagg aatgctcttc tgtatttggg gccgccgttg agtaaagtaa
    42901 acccttctgg cgttatcaac tgcatcggca tcaggtgcgc cagttccgcg actttatgcc
    42961 cgtccgtacc gtcaatacgg actttgctgt cgccctgttt gtctttggtt aagccaatcg
    43021 ccgtctcgcg ctcttcgccc tgtaatcgcc cgtggagaac aaatgcctcc tgctcatggc
    43081 gaatgacgcg accaatctgc aaactgcgaa acgcccgacc atggccgagc gtatagatgg
    43141 cttccagcac gctggttttg ccactgccgt tggcacctac cagaaagtta aagccgggag
    43201 ataaagcgag atccgcggtt tcaatattgc ggaaatcgcg gatcaacaag cgggtgaggg
    43261 acattacagt ctcattggca tgacaacata ggcagccgac tgtgatgcgg catcttcaat
    43321 ctgcacgctg gaaaccgaat cggtcagcat catgcggacg ttttcgcatt tcagcgcgtt
    43381 cagaacatcc agcacatagc tgacgttgaa gccgatttcc atctccgcac cgctataggt
    43441 aacgtcgagg atctcttccg cttcttcctg ttccgggttg ttggcagtaa ttttcagctg
    43501 gttttcgctg acatacaggc gcacaccgcg gaatttctcg ttagagagaa tcgccgcgcg
    43561 agcaaacgcc tgcttgagca gatcgcagcc tgcttccaga tgtttgtccg ggttcttcgg
    43621 cagaacgcga cggtaatccg ggaagcgacc atccaccagt ttcgaggtga aaataaagtc
    43681 gccaacgtgg gcacgaatgt tattgctgcc aatctgcaca cgcagcagat tgtcgccgcc
    43741 gtcgagcata cgcatcagtt caatcacgcc tttacgcggt acgatcaccg aatggcttgg
    43801 caaagattga ccaattggca ttgaacagac cgccagacgg tggccgtcgg ttgccacggt
    43861 gcgcagttct tcaccttcgg tttcaaacag cataccgttt aagtaatagc gaacatcctg
    43921 atgcgccatc gaaaactggg ttgcttcaat cagacgcttc atcgtcgcct gcggcagggt
    43981 aaattcgact tcgctctgcc agtcatcgag gttcgggaaa tccgccgctg gcagggtaga
    44041 cagcgaaaaa cggctacgcc cggagcgcac cagcatccgt tcaccttcca gctgcacggc
    44101 aatttccgcg ccttcaggca gaccacggca gatatcaaag aatttgcgcg ccggaacggt
    44161 cgtcgctcct ggctcgtgtg gctgaaccag cgcaacgcgt gccaccattt ccatctcgag
    44221 atcagtaccg gtcagcgaca acgtaccgtc agcaacctgt aacagcagat taccgagaat
    44281 cggtagcgta ggacgaccac ctaacggacc gctcacctgt tgtagcggtt ttaataaatg
    44341 ctcacgttct acggtaaatt tcataggttt acgatgacaa tgttctgatt aaatttgaaa
    44401 aatcttcttt gatatcgtgg ctctcttcac gcaactgctc gatcttacgg caggcatgaa
    44461 gcaccgtcgt gtggtcacgc ccaccaaacg catcgccaat ctctggcaga ctgtggttag
    44521 tcagctcttt cgctagcgcc atcgccatct ggcgcggacg cgccaccgag cgggatcgac
    44581 gcttggaaag gagatccgcg actttgatct tgtagtactc cgccaccgtc ttctgaatat
    44641 tgtcgatggt gaccagtttt tcctgcaatg ccagcaagtc gcgcagcgcc tcacgcacga
    44701 agtcgatggt gatcgcccgt ccggtaaagt tggcgttagc aataacgcgg ttcagcgccc
    44761 cttccagctc acgtacgtta gatcgtagac gcttggcgat aaagaacgcc acttcacccg
    44821 gcaaacgaat gtcattttcg tcggcctttt tcatcaggat cgccacacgg gtttccagct
    44881 ctggcggttc gatcgccaca gtcagtcccc aaccgaagcg ggatttcaaa cgatcctcaa
    44941 cgccgttgat ctctttcgga tagcgatccg aggtgagaat gatctgttga ttaccttcca
    45001 gcagggcgtt gaaggtgtgg aaaaactctt cctgagatcg ttctttatta gcaaaaaact
    45061 gaatatcgtc gatcagcagt gcatctacgg aacggtagta gcgtttaaac tcttcgatcg
    45121 cgttgttttg cagggcttta accatgtcct gaacaaagcg ctcggagtgc atataaacca
    45181 ctttggcatt cggcttgcgc gccataatgc cgttacccac cgcatgcagc aggtgagttt
    45241 tacccagacc cgtgccgcca taaaggaaca acgggttata ggcaccgcca gggttatccg
    45301 ccacctggcg agccgccgcg cgcgccagtt ggttagattt accttcaacg aagttatcaa
    45361 acgtgtgttt gacgtttacg ttagaacgat aggtcggttc tgccggggcc gggacgttat
    45421 cccaacctga gcgcgtagaa ggcgcagcac gttgcggctg cgtttgcgcc acctgtgcag
    45481 gggccgcgac gttgctcgtc actgccgctt gtggcgtttg cgtcaccggt ttggtgccga
    45541 cttcaaaacg cagctgtggg gcatccgctc cgcagaaact ggttagcagt ccattgatat
    45601 tattaaggta cttgtcccgt acccaatcga ggacaaaacg gtttggcgcg tacagggaca
    45661 gcgtgttatc gctcagttcc gcctgcaatg ggcgtatcca catactgaat tctgtggctg
    45721 gtaactcatc ctgcaatcgg gcaagacact gctgccaaag cgaaagtgac acggcggact
    45781 ccactcgaac aaaagtcgat aatgactaag gctgaaacat tcatgattgt tgacgtacgt
    45841 cgaaaagacc ctgcttgagg gtgacgcacg aaccgctgtc tgcggttata tgcccgatca
    45901 agatcctgca aaacgatcgg gaccgcggat catagcctaa actgcgcaag agatcttctg
    45961 tttctcacag attttcccta tttatccaca ggactttcca gaactcgcta agtgtaaacg
    46021 atcctgccgc gaggcgggca cgatttacgc cgcatattgg aaaatttaat gaccatagac
    46081 aaaaattggc ttaatcgatc taataaagat ccaggacgat ccttgcgctt tacccatcag
    46141 cccgtataat cctccacccg gcgcgccatg ctggtttcca ctggtgtgag gccgtacatt
    46201 ttccctgcga aaaggtgcgg aaaagcgcgg taaataagga aagagaattg actccggtgt
    46261 gtacaattat tacaatccgg cctctttaat cacccatggc ttcggtgtcc atcgtttcat
    46321 ttttcggcgg atatccaata aagccattga atttattcaa gtttaggtag aaatcgccat
    46381 gaaacgcact tttcaaccgt ctgtactgaa gcgcaaccgt tctcacggct tccgtgctcg
    46441 tatggctact aaaaatggtc gtcaggttct ggcacgtcgt cgtgctaaag gccgcgctcg
    46501 tctgaccgtt tctaagtaat aaagctaacc cctgagtggt taagctcgca tttcccaggg
    46561 agttacgctt gttaactccc agtcaattca cattcgtctt ccagcagcca caacgggctg
    46621 gcacgccgca aattaccatt ctcggccgcc tgaattcgct ggggcatccc cgtatcggtc
    46681 ttacagtcgc caagaaaaac gttcgacgcg cccatgaacg caatcggatt aaacgtctga
    46741 cgcgtgaaag cttccgtctg cgccaacatg aactcccggc tatggatttc gtggtggtgg
    46801 cgaaaaaagg ggttgccgac ctcgataacc gtgctctctc ggaagcgttg gaaaaattat
    46861 ggcgccgcca ctgtctcctg gctcgcgggt cctgatagcc ctcattcggg tctatcaacg
    46921 cctgattagt ccgctactcg ggccgcattg tcgtttcact ccaacctgtt caagctacgg
    46981 aattgaggca ttgcgcaggt ttggagtgat aaaaggcagt tggttgacgg tgaaacgcgt
    47041 attaaaatgc caccctttac accctggtgg tgacgatccc gtcccgcccg gaccatttga
    47101 taccagagaa cactaacgat ggattcgcaa cgcaatcttt tagtcatcgc tttgctgttc
    47161 gtgtctttca tgatctggca agcctgggag caggataaaa acccgcaacc tcaggcccaa
    47221 cagaccacgc agacaacgac caccgcagcg ggtagcgccg ccgaccaggg cgtaccggcc
    47281 agtggccagg ggaaactgat ctcggttaag accgacgtgc ttgatctgac catcaacacc
    47341 cgtggtggtg atgttgagca agctctgctg cctgcttacc cgaaagagct gaactctacc
    47401 cagccgttcc agctgttgga aacttcaccg cagtttattt atcaggcaca gagcggtctg
    47461 accggtcgtg atggcccgga taacccggct aacggcccgc gtccgctgta taacgttgaa
    47521 aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc aggtgccgat gacgtatacc
    47581 gacgcggcag gcaacacgtt taccaaaacg tttgtcctga aacgtggtga ttacgctgtc
    47641 aacgtcaact acaacgtgca gaacgctggc gagaaaccgc tggaaatctc ctcgtttggt
    47701 cagttgaagc aatccatcac tctgccaccg catctcgata ccggaagcag caacttcgca
    47761 ctgcacacct tccgtggcgc ggcgtactcc acgcctgacg agaagtatga gaaatacaag
    47821 ttcgatacca ttgccgataa cgaaaacctg aacatctctt cgaaaggtgg ttgggtggcg
    47881 atgctgcaac agtatttcgc gacggcgtgg atcccgcata acgacggtac caacaacttc
    47941 tataccgcta atctgggtaa cggcatcgtc gctatcggct ataaatctca gccggtactg
    48001 gttcagcctg gtcagactgg cgcgatgaac agcaccctgt gggttggccc ggaaatccag
    48061 gacaaaatgg cagctgttgc tccgcacctg gatctgaccg ttgattacgg ttggttgtgg
    48121 ttcatctctc agccgctgtt caaactgctg aaatggatcc atagctttgt gggtaactgg
    48181 ggcttctcca ttatcatcat cacctttatc gttcgtggca tcatgtaccc gctgaccaaa
    48241 gcgcagtaca cctccatggc gaagatgcgt atgctgcagc cgaagattca ggcaatgcgt
    48301 gagcgtctgg gcgatgacaa acagcgtatc agtcaggaaa tgatggcgct gtacaaagct
    48361 gagaaggtta acccgctggg cggctgcttc ccgctgctga tccagatgcc aatcttcctg
    48421 gcgttgtact acatgctgat gggttccgtt gaactgcgtc aggcaccgtt tgcactgtgg
    48481 atccacgacc tgtcggcaca ggacccgtac tacatcctgc cgatcctgat gggcgtaacg
    48541 atgttcttca ttcagaagat gtcgccgacc actgtgaccg acccgatgca gcagaagatc
    48601 atgaccttta tgccggtcat cttcaccgtg ttcttcctgt ggttcccgtc aggtctggta
    48661 ctgtactata tcgtcagcaa cctggtaacc attattcagc agcagctgat ttaccgtggt
    48721 ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat cctgattcgg tgagttttcg
    48781 ctaaaataag ggcggtcagt tgaccgcctt ttttcttttc gtagggcgga taagcaccgc
    48841 gcatccgcca cacaaagcaa caggaacatc atgagcgata atgacactat cgtagcccag
    48901 gccacacctc cgggacgtgg cggcgttggc atcctgcgca tctccggctt caaagcccgt
    48961 gaagttgccg aaaccgtgct gggtaaactg cctaagccgc gctacgccga ttatcttccg
    49021 tttaaagacg ccgacggcag cgtgctcgat caggggattg cgctatggtt ccctggcccg
    49081 aactcgttca ccggcgaaga tgtgctggaa ctgcaaggtc atggcggtcc ggtgatcctc
    49141 gacctgctgt taaaacgcat tctgaccatt cccggcctgc ggattgctcg ccctggtgag
    49201 ttttccgaac gcgcgtttct taacgataaa cttgacttag cccaggccga ggcgattgcc
    49261 gatcttatcg acgccagttc ggaacaggcg gcccgttcgg cacttaactc gctgcaaggc
    49321 gcattctccg cacgggttaa tcatctggta gaagccctca cccacttgcg catttacgtc
    49381 gaagcggcaa ttgatttccc tgatgaagag atcgatttcc tctccgacgg caaaattgaa
    49441 gcccagctta atgacgtgat ggccgatctc gatgcagtgc gtgctgaagc acgtcagggt
    49501 agtttgttgc gcgaagggat gaaagtagtg attgccggac gtcctaatgc cggtaaatcg
    49561 agcctgttaa acgcgctggc gggccgtgaa gcggcaatcg taaccgatat cgccggaacc
    49621 acgcgtgacg tgctgcgtga gcatatccac attgacggaa tgccgctgca tatcatcgac
    49681 actgccgggc tacgtgaggc cagtgacgaa gtggaacgta ttggtatcga gcgcgcgtgg
    49741 caggaaattg aacaggccga ccgcgtgctg tttatggtcg atggcaccac gacagacgcc
    49801 gttgatccgg cagagatctg gccggaattt atcgcccgtc tgcccgcgaa actgccgatc
    49861 accgtggtac gcaataaagc cgatatcacc ggcgaaacgc tgggaatgag tgaagtgaac
    49921 ggtcacgcgt taattcgtct ctcggcaagg actggtgaag gcgtggacgt gctgcgtaac
    49981 catctcaaac agagcatggg ctttgacacc aatatggaag gcggcttcct ggcgcgccgt
    50041 cgccacttac aggcgctgga acaggcagcg gagcatttgc agcaaggtaa agcgcaactg
    50101 ttgggagcct gggcaggtga actgctggcg gaagagttgc gcctggcaca gcagaactta
    50161 agcgaaatca ccggggaatt tacttcagac gacctgctgg ggcggatttt ctccagcttc
    50221 tgtattggta agtaaccgcg cttacgaagc cgtattctga ctgtcagatg cggcttcgct
    50281 tcattgttag cactcctgtt atgcctcaac ccttttttaa acattaaa
//
LOCUS       DADLTF010000011        49498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.011, whole genome shotgun sequence.
ACCESSION   DADLTF010000011 DADLTF010000000
VERSION     DADLTF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.011"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(129..608)
                     /gene="folK"
                     /locus_tag="JN749_000645"
     CDS             complement(129..608)
                     /gene="folK"
                     /locus_tag="JN749_000645"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HAY7367158.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN749_000646"
     CDS             complement(605..2023)
                     /gene="pcnB"
                     /locus_tag="JN749_000646"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HAY7367159.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN749_000647"
     CDS             complement(2062..2988)
                     /gene="gluQRS"
                     /locus_tag="JN749_000647"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706092.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HAY7367160.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3025..3480)
                     /locus_tag="JN749_000648"
     CDS             complement(3025..3480)
                     /locus_tag="JN749_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HAY7367161.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN749_000649"
     CDS             complement(3658..4362)
                     /gene="sfsA"
                     /locus_tag="JN749_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAY7367162.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN749_000650"
     CDS             complement(4377..4907)
                     /gene="thpR"
                     /locus_tag="JN749_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HAY7367163.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4981..7410
                     /gene="hrpB"
                     /locus_tag="JN749_000651"
     CDS             4981..7410
                     /gene="hrpB"
                     /locus_tag="JN749_000651"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HAY7367164.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7612..10146
                     /gene="mrcB"
                     /locus_tag="JN749_000652"
     CDS             7612..10146
                     /gene="mrcB"
                     /locus_tag="JN749_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HAY7367165.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLAAIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10366..12609
                     /gene="fhuA"
                     /locus_tag="JN749_000653"
     CDS             10366..12609
                     /gene="fhuA"
                     /locus_tag="JN749_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HAY7367166.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FGENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12660..13457
                     /gene="fhuC"
                     /locus_tag="JN749_000654"
     CDS             12660..13457
                     /gene="fhuC"
                     /locus_tag="JN749_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HAY7367167.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13457..14347
                     /gene="fhuD"
                     /locus_tag="JN749_000655"
     CDS             13457..14347
                     /gene="fhuD"
                     /locus_tag="JN749_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HAY7367168.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14344..16326
                     /gene="fhuB"
                     /locus_tag="JN749_000656"
     CDS             14344..16326
                     /gene="fhuB"
                     /locus_tag="JN749_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HAY7367169.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="JN749_000657"
     CDS             complement(16361..17641)
                     /gene="hemL"
                     /locus_tag="JN749_000657"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HAY7367170.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLMTEL
                     VPTIDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17866..19287
                     /gene="clcA"
                     /locus_tag="JN749_000658"
     CDS             17866..19287
                     /gene="clcA"
                     /locus_tag="JN749_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HAY7367171.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGVLVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19311..19376
                     /locus_tag="JN749_000659"
     CDS             19311..19376
                     /locus_tag="JN749_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367172.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19369..19713
                     /gene="erpA"
                     /locus_tag="JN749_000660"
     CDS             19369..19713
                     /gene="erpA"
                     /locus_tag="JN749_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HAY7367173.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19760..20383)
                     /locus_tag="JN749_000661"
     CDS             complement(19760..20383)
                     /locus_tag="JN749_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HAY7367174.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="JN749_000662"
     CDS             complement(20421..21221)
                     /gene="btuF"
                     /locus_tag="JN749_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HAY7367175.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="JN749_000663"
     CDS             complement(21214..21912)
                     /gene="mtnN"
                     /locus_tag="JN749_000663"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAY7367176.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21996..23513
                     /gene="dgt"
                     /locus_tag="JN749_000664"
     CDS             21996..23513
                     /gene="dgt"
                     /locus_tag="JN749_000664"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7367177.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23643..25067
                     /gene="degP"
                     /locus_tag="JN749_000665"
     CDS             23643..25067
                     /gene="degP"
                     /locus_tag="JN749_000665"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HAY7367178.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25222..26378
                     /locus_tag="JN749_000666"
                     /pseudo
     CDS             25222..26378
                     /locus_tag="JN749_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
     gene            complement(26467..26853)
                     /locus_tag="JN749_000667"
     CDS             complement(26467..26853)
                     /locus_tag="JN749_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HAY7367179.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="JN749_000668"
     CDS             complement(27167..27991)
                     /gene="dapD"
                     /locus_tag="JN749_000668"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HAY7367180.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="JN749_000669"
     CDS             complement(28022..30694)
                     /gene="glnD"
                     /locus_tag="JN749_000669"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HAY7367181.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30756..31550)
                     /gene="map"
                     /locus_tag="JN749_000670"
     CDS             complement(30756..31550)
                     /gene="map"
                     /locus_tag="JN749_000670"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAY7367182.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31918..32643
                     /gene="rpsB"
                     /locus_tag="JN749_000671"
     CDS             31918..32643
                     /gene="rpsB"
                     /locus_tag="JN749_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAY7367183.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32901..33752
                     /gene="tsf"
                     /locus_tag="JN749_000672"
     CDS             32901..33752
                     /gene="tsf"
                     /locus_tag="JN749_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAY7367184.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33899..34624
                     /gene="pyrH"
                     /locus_tag="JN749_000673"
     CDS             33899..34624
                     /gene="pyrH"
                     /locus_tag="JN749_000673"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAY7367185.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34916..35473
                     /gene="frr"
                     /locus_tag="JN749_000674"
     CDS             34916..35473
                     /gene="frr"
                     /locus_tag="JN749_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAY7367186.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35565..36761
                     /gene="ispC"
                     /locus_tag="JN749_000675"
     CDS             35565..36761
                     /gene="ispC"
                     /locus_tag="JN749_000675"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HAY7367187.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36950..37708
                     /gene="ispU"
                     /locus_tag="JN749_000676"
                     /gene_synonym="uppS"
     CDS             36950..37708
                     /gene="ispU"
                     /locus_tag="JN749_000676"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HAY7367188.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37721..38578
                     /gene="cdsA"
                     /locus_tag="JN749_000677"
     CDS             37721..38578
                     /gene="cdsA"
                     /locus_tag="JN749_000677"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAY7367189.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWCADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38590..39942
                     /gene="rseP"
                     /locus_tag="JN749_000678"
     CDS             38590..39942
                     /gene="rseP"
                     /locus_tag="JN749_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HAY7367190.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39972..42404
                     /gene="bamA"
                     /locus_tag="JN749_000679"
     CDS             39972..42404
                     /gene="bamA"
                     /locus_tag="JN749_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HAY7367191.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42526..43011
                     /gene="skp"
                     /locus_tag="JN749_000680"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42526..43011
                     /gene="skp"
                     /locus_tag="JN749_000680"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HAY7367192.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43015..44040
                     /gene="lpxD"
                     /locus_tag="JN749_000681"
     CDS             43015..44040
                     /gene="lpxD"
                     /locus_tag="JN749_000681"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HAY7367193.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44145..44600
                     /gene="fabZ"
                     /locus_tag="JN749_000682"
     CDS             44145..44600
                     /gene="fabZ"
                     /locus_tag="JN749_000682"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAY7367194.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44604..45392
                     /gene="lpxA"
                     /locus_tag="JN749_000683"
     CDS             44604..45392
                     /gene="lpxA"
                     /locus_tag="JN749_000683"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HAY7367195.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45392..46540
                     /gene="lpxB"
                     /locus_tag="JN749_000684"
     CDS             45392..46540
                     /gene="lpxB"
                     /locus_tag="JN749_000684"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HAY7367196.1"
                     /translation="MTDQRPLTIALVAGEISGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46537..47133
                     /gene="rnhB"
                     /locus_tag="JN749_000685"
     CDS             46537..47133
                     /gene="rnhB"
                     /locus_tag="JN749_000685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAY7367197.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47170..>49498
                     /gene="dnaE"
                     /locus_tag="JN749_000686"
     CDS             47170..>49498
                     /gene="dnaE"
                     /locus_tag="JN749_000686"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAY7367198.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKA"
BASE COUNT        11593 a        12205 c        13755 g        11945 t
ORIGIN      
        1 atgggattga tatttatctg cgggaattta tattatgttt ctgcaaaaat gtttaaacta
       61 aaatttttat taaggaaaga gaggatgtat gattttcaga aaatatttat attcgtaata
      121 taaataaatt accatttgct taatttgtca aatgctcttg tatgtaagac ttcacgcaac
      181 gtctccccat caggaaacgc cagctccggt gcaatctcaa acagcggcca cagcataaat
      241 ccacgattct tcatatcgta gtgcggaacg gtcaggcgtt cggtatttat cacttcatta
      301 ccaaacagca tgatatcgag atccagcgtg cgtggtcccc agcgttcagc tttacgaaca
      361 cgaccctgtt gcagttctat gcgctgtgta tggttgagta actcttccgg ggcgggcgaa
      421 gtttcaagcg ccacggcggc gttgaggtaa tcgggttgat cttgcggccc cagcggtggg
      481 gtgcggtaaa acgaagaaac ggcaagaatg cggctttcag ggatatcgcc taatgctttc
      541 agggcagcat tgacctgctc cagcggagag gccagattgc tgcctatggc aatatacgcc
      601 actgtcatgt ggtaccctca cgacgcggtg cgcgtttgcg tggacgacga gtacgacgac
      661 gcggtgacgg ttcttcatcc agctcgttaa gcatcccttt ttgatctggt ggtgcggaaa
      721 cctggaactc accccaccat ttcaccagac gctgcagttc agcattacgt tcaacttcag
      781 ctcgcaaggc caacaggtca taagccgcac ggaacttagg atgctccagc agtttccatg
      841 cgcgtttacc ctgacgacgg gacatacgca actgcaactg ccagatatcg cgggttaacg
      901 tcgtcagacg tttcgggatt gccagtgaac ggcaggcttc gtccagcacg tcgttcatcg
      961 ccagcgcgaa agcgtcgtga taggtcaggc cgctttcctg ggcgatcttc tgtgccgtct
     1021 ccagcagtgg gtaccagaac atggcggcaa acaagaacgc cgggttcacg cgcatatcgt
     1081 tatggatacg cgtatcggta ttcttcagca cctgttcaat gatccgctcc atcgggctgt
     1141 cgccattttc cgtgaagtag cgggtaatgg tcgggaacag cggctggaac agatgatatt
     1201 cacacaacag cttataggtt tcgtaaccgt agcccgcttg tagcagttta agcgattctt
     1261 caaacaggcg tgccggtggg atatcgttca gcagggtagc gaggcgaggg atcggttctg
     1321 cggtttccgg gctgatgcgc atacccaatt tggcggcaaa acgtaccgcg cgcagcatac
     1381 gtaccggatc ttcacggtag cgcgtttccg ggttaccaat cagacggata acgccgtcct
     1441 tcagatcctt cataccgcca acgtaatcac ggacggtaaa atccgctacg ctgtaataca
     1501 ggctgttgat agtgaaatcg cggcgctggg cgtcttcttc gatggagccg aaaatgttgt
     1561 cgcgcagcaa catgccgttt tgcccgcgtt gggaggtcgt gcggtcgctg acgttacctt
     1621 cgtggtgtcc acggaaggtc gcaacttcga taatctccgg gccaaacatt acatgagcca
     1681 gacggaaacg gcgacccacc aggcggcagt tacggaacag tttgcgcacc tgctcaggcg
     1741 tggcgttagt ggttacgtca aaatctttcg gctttttgcc aagtaacagg tcgcgcacgc
     1801 cgccgccaac cagccaggct tcgtatcccg ctttgttgag cctgtacatt accttcaggg
     1861 cattttcact gatatctttg cgggaaatag catgctgctc acgcgggatc accgtcacct
     1921 gtggacgggc gactgcctgt tcagcctcgc tttcctcgcg gcttagcacc ttgcggcaaa
     1981 aattagcgac tcgggtaaaa atagtacacc tcggtagtgt caaacatcat tcaggacaaa
     2041 aaaatagcgg ctaatcatag ctcagcatga cgcatttgag aatgttgaat ttacaattgc
     2101 cgactcgggc acggcggtca gcatccagtt tttgacggct gactgaagga tttgctcgac
     2161 gctgaaatcc tgccagtgtg cttctgcctg ctgccccaga aattgaagtg ccgcgattag
     2221 taccgggcgt ggatcgcctt tcggcaacgc aggcgcatga ttctgcttgg aaagtttagc
     2281 gccttgtgga ttaagcgcca gcggcagatg aatgtaatct ggcactttcc agccaaaaag
     2341 ctggtacagc gagatttgcc ttactgttgg ttcaatcaga tcagccccac gcactatttc
     2401 tgtaacgccc tggaaatgat catcaaccac aacagccagg ttgtaggcga acaacccatc
     2461 acggcgatga atgataaaat cttcccgtgc cagtttttcg tcggcgtgaa taatgcctcg
     2521 cagcaggtca gtaaattgcg tgaccggatg ctgctggcgg atacgcactg cggcgttgtc
     2581 tggtccatga tgcaacaccc ggcaatgacc gtcgtaaata ccgccaatgc tttgaatacg
     2641 cgcacgcgta caggtgcagt aataacttag tccttgttca tgtaaccagg cgagtgcttc
     2701 acgataggcg tcgtgacgtt gcgattgcca gagaacatcg ccgtcccagt gcagaccgta
     2761 atgttccagc tggcgcagga tagtttctgc ggcaccggga acttcacgag gcgggtcgat
     2821 atcttctatg cgtaccagcc agcgaccttg ccgggcgcga ccctgcaaat agctgccgag
     2881 cgcggcgatc agagagccaa aatgaagctc gccggaagga gagggggcga agcggccaat
     2941 atactgtgtg tctgtcatct ctttgaacaa aaaataaggc gggagcattt cccgcctgtg
     3001 gtaaacatga tggaacggct gtaattagcc agccatctgt ttttcgcgaa tttcagccag
     3061 cgttttgcag tcgatgcaca gatcggctgt cgggcgcgct tccagacggc gaataccaat
     3121 ttcaacaccg caggattcgc agtagccgaa atcttcgtct tccacttttt tcagcgtctt
     3181 ctcgatcttt ttgatcagct tacgctcgcg atcgcggtta cgcagttcga ggctgaactc
     3241 ttcttcctgg gctgcacggt ctaccgggtc cgggaagttg gctgcttcat cctgcatatg
     3301 tgtaacggtg cgatcgactt catccctgag ttgattacgc catgcttcca gaatacgacg
     3361 gaagtgcgcc agctgggctt cattcatata ctcttcgccc ggcttctcct gatatggttc
     3421 caccccagcg atggcgagaa tactcaggga cgatgtttta cggttttgcc cttcttgcat
     3481 gttgcttctc cttaacacgc actatcgatc cccatgttcg ggggaaaaat gaggccgcta
     3541 taaatagcag atgcttttcc ggatagcaat tatctaaacg taacacttga caactgtgtg
     3601 aggaaaagcg tattcgcgca cgcgaccaga atgtaaatta accagttact tactttacta
     3661 caatgtaacc ggcagtgatt ttttaagagc catgccttca gcagaaattt ccgctttgta
     3721 agccagaatt tctacccccc tctgttgagc ttctgacaat agttgcgcgt atttctcatc
     3781 gatgtggcgc gcgggtgaaa accgtgtaat ggctgaatgc agcacggcga aaaagataac
     3841 cgcacgctgg ccttcagccg ctacgctcat caactcccga aggtgtttct gacctcgttc
     3901 agtgaccgca tcgggaaaat atccctgttc gttctccgct aacgtaaccg atttcacttc
     3961 aatatagcag tctggacgcg aatccgcctg caacataaag tcaatacggc tgcgttcgga
     4021 gccgtatttt acttcgcttt tcagcgagct atagcctgac agttctgaaa ttgattcatt
     4081 aaggatagcc tctttcgtca acctgttagc ccaaagcgta ttgacgcaaa taaatgcgcc
     4141 gctctggctt tgagttaatt cccaggtgtg tgggtatttc cgtttggtgt tgtctgaagt
     4201 cgaataccag acggtatcgc caggcgttgc acaaccggtc atcgcacccg tattcgggca
     4261 gtgtagcgtt aattcgcgac catcgggtgt gatcacatcg gctaaaaaac gtttgtaacg
     4321 ctgaattagc gtcgcgcgct gtagaggggg agaaaattcc attgcgacaa tccttgttat
     4381 tgcgttagcg cccagcgttt tagcggcgtg tagcgtgtgc gtccacgggc aaacgaggag
     4441 gcgtaaaggg tgaactccgt caccgcataa gaccagttaa aacccggcgg cgggaatgtc
     4501 accgcctcgc tggcgtcgcg caataaggta atatgtggat gaaacggacg attgctttga
     4561 aaacaaccgc tgcgggcagc ctgtgaacgg agcatattcg ccagctggat taagccgcgt
     4621 ggaggctgac gcatccctaa ccacaccaca cgcgaacgca gccattgtcc ggcgtcatca
     4681 agcgtgagtg tgaaacctgg ttgacgaatc cgtccggcta aaagagaaag cgccttctct
     4741 ttctctgcgc tcacttcgcc taaaaatgcc agagtcagat gcaaattatc ggcggcgacc
     4801 ggacgtcccg cctcaggtgg gaagtgtgtg gcgcgccaat ggataatctg ttcgcgggtt
     4861 tctgcaggta agtcgatagc aaagaacaga cgttgcggtt cagacatgtg aggcactcgg
     4921 ttatgaatta ccgcgatgct acaatgtggc gcgaagaatg ttaaccctct ggagcgtttt
     4981 gtgtcgtcgt tgcccgttgc tgccgtctta cctgaattac ttaccgctct cgattgtgcg
     5041 ccgcaggtat tattaagtgc gccgaccggg gccgggaaat caacctggct gccgctgcaa
     5101 ctgctggcgc atcccggcat taacgggaaa attatcctgc tggagccgcg tcgtctggcg
     5161 gcgcgtaacg tcgcgcaacg gctggcggag ctgcttaacg aaaagccagg cgataccgtt
     5221 ggctaccgga tgcgtgcgca aaactgcgtc gggccgaata cccgcctgga agtggttacc
     5281 gaaggcgtgc tgacgcggat gatccagcgt gacccggaac tgagcggtgt tggattggtg
     5341 atccttgatg agtttcatga gcgcagtttg caggcggatt tggcgttggc gctgttactc
     5401 gatgtgcaac aaggtctgcg tgatgacctt aaactgctga ttatgtcggc tacgctggac
     5461 aatgaccgct tgcagcaaat gctgccggaa gcgcccgtcg tcatctcaga agggcgctcg
     5521 tttccggttg aacgccgtta tttaccgctg cccgcgcatc agcgttttga cgaagccgtt
     5581 gcggtagcca ccgccgaaat ggtgcgtcag gaaagtggat cattactgtt atttttacct
     5641 ggcgtcggag aaattcagcg cgtgcaggaa caactggctt cgcgcatcgg cagtgatgtg
     5701 gtgctctgcc cgctgtatgg cgcgttgtca ctgaacgatc agcgaaaagc gatcctcccg
     5761 gcaccgcaag ggatgcgcaa agtggtgctg gcgaccaata ttgctgaaac cagtttaacc
     5821 atcgaaggta ttcgtctggt ggtggattgt gcccaggagc gtgtggcgcg ctttgatccg
     5881 cgcacggggc ttacgcggtt gattactcaa cgcgttagtc aggcatccat gacgcagcgt
     5941 gccgggcgcg ccgggcgtct ggagccgggt atcagcctgc atttaatcgc caaagaacaa
     6001 gcagaacgcg ccgcggcgca aagtgagccg gagatcttac aaagcgatct ttccggtttg
     6061 ctgatggaat tactgcaatg gggatgcagc gatccggcgc agatgagctg gctggatcaa
     6121 ccgccaacgg tgaatctact ggccgcgaaa cgcctgttac aaatgctggg ggcgctggag
     6181 ggtgaacggc ttagtgcgca agggcaaaaa atggcagcgc tgggtaacga tccgcgttta
     6241 gcggcaatgc tggttaacgc aaagagcgat gacgaagctg ctaccgcggc aaaaattgcc
     6301 gccattctcg aagagccgcc acggatgggc aatagtgacc tgggcgtggc gttttcgcgc
     6361 aatcaaccag cctggcagca acgtagtcag caactgttaa aacgcttaaa cgtacgcggc
     6421 ggtgaggcag acagttcgct tatcgcgccg ctacttgccg gggcgtttgc cgatcgcatt
     6481 gctcatcgcc gtgggcaaga tggacgctat caactggcaa acggcatggg agcgatgctc
     6541 gatgccgacg acgcgctaag ccgccacgaa tggttgatcg caccgttatt attgcagggc
     6601 agcgcctcgc cggatgcgcg gattttactg gcgctaccgg tcgatattga tgaattagta
     6661 caacgctgtc cgcagctggt gcagcaatct gacaccgtgg aatgggatga cgcgcaaggt
     6721 acgctgaaag cctggcgtcg gctacaaatc ggtcagttga cggtgaaagt gcagccgctg
     6781 gcgaaaccgt cagaagacga gttgcatcag gcgatgctta atggcatccg tgataaaggt
     6841 ttaagcgtgc tcaactggac ggcggaagcg gaacagctac gcttgcgttt gttatgcgcc
     6901 gcaaagtggt tgccggaata tgactggcca gcggttgatg atgaaagttt gttggcgacg
     6961 ctggaaacgt ggctgctgcc acatatgacc ggcgtacatt cactacgcgg cctgaaatca
     7021 ctcgacattt atcaggcact acgcggatta cttgattggg gaatgcagca acgtctggat
     7081 agtgaattgc ctgcgcatta cactgtgccg acgggaagcc ggatcgccat tcgttatcat
     7141 gaagataacc cgcccgcgct ggcggtgaga atgcaggaga tgtttggtga ggccactaat
     7201 ccgacgatcg cccaggggcg cgtgccgctg gtgctgaagt tgctttcccc tgcccaaagg
     7261 ccgctgcaaa tcacgcgtga tttgggggcc ttctggaaag gggcgtatcg tgaggtgcaa
     7321 aaggagatga aagggcgtta tcccaaacac gtctggccga acgacccggc aaacacggca
     7381 ccgacgcgac ggacgaaaaa gtattcgtaa ggttggtttt ctccctctcc ctgtgggaga
     7441 gggccggggt gagggcatca gcgcgcacgt tcaccctcac cctaacccta accctctccc
     7501 tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa
     7561 cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag catggccggg
     7621 aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta
     7681 agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta tgaggatgaa
     7741 gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc
     7801 ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat cgccatttac
     7861 ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg gcaactgccc
     7921 gcggcagttt atggccgaat ggtcaacctt gaaccagata tgaccatcag caagaacgaa
     7981 atggtgaagc tgctggaggc aacacagtat cgtcaggtgt cgaaaatgac ccgtcctggc
     8041 gaatttaccg tgcaggccaa cagcattgag atgatccgcc gtccgtttga tttcccggac
     8101 agtaaagaag ggcaggtgcg tgcgcgtctg acctttgatg gtgatcatct ggcggccatc
     8161 gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc
     8221 atgatctcct cgccaaacgg tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat
     8281 ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca tgatggaatc
     8341 agtctctact ccattgggcg tgcggtgctg gcaaacctga ccgcaggtcg cacggtgcag
     8401 ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag cgagcgttct
     8461 tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg ttacagcaaa
     8521 gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa
     8581 atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga agagctaagc
     8641 ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta caacccgtgg
     8701 cgtaacccga aactggcgct ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa
     8761 cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg ggttcagccg
     8821 cgtggtgggg tgatctctcc tcagccagcc tttatgcagt tggtgcgtca ggagttgcag
     8881 gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac tacctttgac
     8941 tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc actgaagaaa
     9001 cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa
     9061 gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag
     9121 gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc tttaagccag
     9181 ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct gcgtcagccg
     9241 aatggccagg tctggtcacc gcagaacgat gaccgtcgtt atagcgaaag cggcagagtg
     9301 atgctggtgg atgcgttgac acgttcgatg aacgtgccga cggtaaatct ggggatggcg
     9361 ctggggctgc ctgcggttac ggagacctgg atcaaactgg gcgtaccgaa agatcagttg
     9421 catccggttc cggcaatgct gctgggggcg ttgaacttaa cgccaatcga agtggcgcag
     9481 gcattccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact gcgttcggta
     9541 atcgcggaag atggtaaggt gctgtatcag agcttcccgc aggcagaacg cgctgttccg
     9601 gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg tggtacgggt
     9661 cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg gactaccaac
     9721 aataacgtag atacctggtt tgcaggcatt gacggcagca cggtgaccat cacctgggta
     9781 ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat gtcgatttat
     9841 cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc agaagatatt
     9901 gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg
     9961 ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca gcagcagccg
    10021 tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca gcaacctgct
    10081 cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat gtttggtagt
    10141 aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa ataacatccc
    10201 atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca ggaacgctca
    10261 gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct ttataataat
    10321 cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc gcgttccaaa
    10381 actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac agcggttagc
    10441 ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat caccgttacc
    10501 gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc ggcgcggcag
    10561 tcagctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc tatttctgtt
    10621 gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc gcttagctac
    10681 acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca cctgatcatt
    10741 cgcggttttg cggcagaagg ccaaagccaa aataactatc tgaatggcct gaagttgcag
    10801 ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc tgaaattatg
    10861 cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggtctgtt gaatatggtc
    10921 agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg tactgacagc
    10981 ctgttccaga ctggttttga ctttagcgat gcgttggatg atgacggtgt ttactcttat
    11041 cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga agagcagcgt
    11101 tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt caccttcctt
    11161 tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa agagggaacc
    11221 gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg ggcgaagaac
    11281 aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga atttaacgac
    11341 acctttactg tgcgtcagaa cctgcgcttt ggtgaaaaca aaacctcgca aaacagcgtt
    11401 tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg tgcggcatta
    11461 gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga tgagaagctg
    11521 caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga tatcgaccac
    11581 accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc ctggtttggt
    11641 tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaatcccga tttcgacttc
    11701 aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca gaaacaaacg
    11761 ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct aggcggtcgt
    11821 tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga taaacgtgat
    11881 gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg tgtaacacct
    11941 tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga tggtaatatt
    12001 ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc ggaagatcgt
    12061 ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct gatggcggac
    12121 cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg cgtagaaatc
    12181 gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac ttacaccgat
    12241 gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt gccaaaacac
    12301 atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg tttgacgctg
    12361 ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc ctttaaagtg
    12421 ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt cggcatggcg
    12481 ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt cgccagctgc
    12541 tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac cgcaaccttt
    12601 cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag ttggctgtta
    12661 tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc tcctttcgtg
    12721 tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg aaagtgaccg
    12781 gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt ggtcgtcatc
    12841 agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc tggagcagca
    12901 aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca gaagggatga
    12961 ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg gggcgctttg
    13021 gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta aaaccgctgg
    13081 cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg atcgccatgc
    13141 tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg ctggatatcg
    13201 cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt ggcctgacgg
    13261 tcattgccgt gttgcacgat atcaatatgg cggcacgcta ctgtgattat ctggtcgccc
    13321 tgcgcagtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc ggcgaaaccc
    13381 tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt gctgcacctg
    13441 tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac tgttaacggc
    13501 gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg ctattgaccc
    13561 caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc tcggcatcgt
    13621 gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac caccattgcc
    13681 ggactcagtg attgacgtcg gtttgcgtac agaacctaac cttgaactgc tgaccgaaat
    13741 gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcatcagaaa tgctggcacg
    13801 tattgcgccg ggtcgcggat ttaacttcag tgacggcaaa catccgttgg cgatggcgcg
    13861 taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcgg aaacgcattt
    13921 agcgcaatac gaagacttta tccgcagcat gaaaccccgc tttgtgaagc gtggtgcacg
    13981 cccgttactg ctgacgacgc ttatcgatcc gcgccatatg ctggtcttcg gtccaaacag
    14041 cttgttccag gaaattctcg atgagtacgg catcccaaat gcctggcaag gggaaaccaa
    14101 cttctggggc agtaccgccg tcagtatcga tcgtctggca gcgtataaag acgttgatgt
    14161 gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa ccccgctgtg
    14221 gcaggccatg ccgtttgtcc gtacagggcg ctttcagcgc gtacccgcag tctggtttta
    14281 tggtgcaacg ctttcggcaa tgcactttgt gcgtgttctg gataacgcca tcggaggtaa
    14341 agcgtgagta aacgaattgc gcttttcccg gcgttattgc tggcgctgtt agtgattgtc
    14401 gctacggcgc tcacctggat gaacttctca caggcgctgc cgcgcagtca gtgggcgcag
    14461 gccgcctggt cgccggatat tgacgtcatc gagcagatga tttttcacta cagcttgttg
    14521 ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg cgtgctgttt
    14581 cagcaagtac tgcgtaaccc gctggcggaa ccgacgacgt tgggcgttgc aacaggcgcg
    14641 caactgggga ttaccgtcac cacgctctgg gcgatcccta gcgcgatggc gagccagttt
    14701 gctgcgctgg caggggcttg cgttgttggc ttaattgtct ttggcgtcgc gtgggggaaa
    14761 cggctgtcgc cggtaacgct gattctcgcg gggttggtag tgagccttta ttgcggcgca
    14821 atcaatcagt tactggttat cttccatcat gaccagttgc aaagcatgtt tctgtggagc
    14881 actggaacgc tgacgcaaac cgactggggc ggtgttgagc gtttatggcc gcagttgctg
    14941 ggcggtgtga tgctgacgtt gctgctactt cgtccgttaa ccctgatggg gcttgatgat
    15001 ggcgtggcgc gcaatctcgg gctggccttg tcgcttgcgc gtctggcggc gctgtcgctg
    15061 gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt tatcgggttg
    15121 ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg actgatgctg
    15181 gcgtcgttga ttggtgcgct gatcctctgg ctttccgatc aaatcatcct ctggctgact
    15241 cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc gccgctgcta
    15301 ctgtggctgc tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt taacgatcgt
    15361 gtcgcgactg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt gctgttgttg
    15421 atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac gtgggcgagc
    15481 ggggcgttgc tcgaggattt aatgccctgg cgctggccgc gaattatggc ggcgctgttt
    15541 gcgggcgtca tgctggcggt agcgggctgt attattcagc gactgaccgg caacccgatg
    15601 gcaagcccgg aagtgctggg gattagctcc ggcgcggcgt ttggtgtggt gttaatgctg
    15661 tttctggtac cgggtaatgc ctttggctgg ttgttacccg caggcagtct cggggcggcg
    15721 gtgatgctgt tgatcattat gatcgccgcc ggacgcggtg gattttcccc acaccgcatg
    15781 ttactggcgg ggatggcgtt aagtaccgcg ttcaccatgc ttttgatgat gttgcaggca
    15841 agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac ctacaacgcg
    15901 accgatgctc aggtctggcg caccggaatt gtgatggtga ttttgctggc gattactccg
    15961 ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcgcg ggcggtaggg
    16021 atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct gacggcgacc
    16081 gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca tattgcgcgg
    16141 atgatgggct ttcgacggac gatgccacat atcgtaattt cggcgctggt gggtggttta
    16201 ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca gatcccggcg
    16261 gggctgctgt cgacctttat cggtgcgcca tattttatct atttgctgag aaagcagagc
    16321 cgttaataga atgcctcgcc cggtgggcga ggcaaaatta tcacaacttc gcgaacaccc
    16381 gacgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccaca gacataaagc
    16441 ccgcttcaaa cgctgacggt gccaggtaaa caccttcatc cagcatcata tggaagaaac
    16501 gcttaaaacg ctcgacgtca caggccatca catcctgata gcacgtcacg gactcggcgt
    16561 cggtaaagaa gataccgaac ataccaccga cgtggttaac gaccagcgga attcccgctt
    16621 cttctgccgc ttccagcaga ccttctgcca gacgtgttgt cagctcatcc agcgtttcgt
    16681 gaatgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc gcaatcgggt
    16741 taccggaaag cgtacccgcc tgatagaccg gacctgtagg ggccagcgca tccattacat
    16801 cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttt ccgaggcagg
    16861 ttaaatctgg cactacgccg aaataatcct gtgcgccagc tagcgctacg cggaaaccgg
    16921 tcatcacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgca cgcagacctg
    16981 gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc tcgacgataa
    17041 tacaggcaat ctcttgcggg tattgctcaa acgcggcgcg tacagaggcc agatcgttat
    17101 aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag tttggctggc
    17161 ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga ccgtggtaac
    17221 aaccttcaaa tttaatgatt ttgtcgcgac cggtaaaacc acgggccaag cggatggcgc
    17281 tcatcgtcgc ctcggtgccg gagttcacca tgcgcaccat atctatggtc gggaccagtt
    17341 cggtcatcag ttgcgccatt ttcacttcca tttcggtcgg tgcaccaaag cttaaaccac
    17401 gctcggcggc ttcaatcacg gcattgcgga tcgccggatg gttatggccc agcaccatcg
    17461 gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac agataagcgc
    17521 cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg cgaacagggg
    17581 agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca gacttactca
    17641 tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta ttcataacaa
    17701 gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt tcttagagta
    17761 taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg aacgtgcgag
    17821 taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa aactgatact
    17881 ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat tcgccaactt
    17941 cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt cggcacgctt
    18001 atcgggctgg ctgcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa ccaacgtatg
    18061 ggagtgctgg tacatactgc tgataattat ccgctactgt taaccgtcgc ttttctctgt
    18121 tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc cgaagcaggg
    18181 ggatcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcctgt tcgctggtgg
    18241 cgtgtattgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg catggtgcta
    18301 gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt gcttgatatt
    18361 ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc tgctgcgggg
    18421 ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga agagatgcgt
    18481 ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt cattatgtcg
    18541 accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg taaactttct
    18601 gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt tggcattttc
    18661 ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg tgtgcacggc
    18721 ggcaatatta ccaaatgggt actgatgggg ggggcgattg gcggtctgtg tggattgctg
    18781 gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat cgctaccgcg
    18841 gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat taccacctta
    18901 ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc gctgggtact
    18961 gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata tcacctggag
    19021 gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat tcgcgcgccg
    19081 ttaacgggga tcattctggt tctggagatg accgataact accagctcat tttgccaatg
    19141 attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa acctctatac
    19201 tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc gcgaagcaag
    19261 gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata atggcgatta
    19321 ttattgggtt agaatttgcc cgattgccga tgtcgtttgg agcaaaatat gagtgatgac
    19381 gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag cctgatcgct
    19441 gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg ttgcagcggc
    19501 ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac catcgaaaaa
    19561 cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg cggttccgtt
    19621 gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa cgcgaaaagc
    19681 acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc ggataaacta
    19741 tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc ggtagcttaa
    19801 gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc atcatactgg
    19861 ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata ccgccgataa
    19921 tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatttcgcgg gccagtacat
    19981 cacgaatgat cccgccgcca acgccagtaa tgacgcccat acaaaccgcg attaacggac
    20041 cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc agaccaacgg
    20101 cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc agcacgatgg
    20161 tcagcatgct ggtgaccatg gcaacgacca gatcggtggg atctttcacc caaaataccg
    20221 ggccgtgatc cagcgccatg tcgcgaattg ttccgtcgcc tactgcggta accacgccca
    20281 gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg ccggagatgg
    20341 caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa tccccactga
    20401 gcacctgaaa aggtcaggca ctaatctacc tgtgaaagcg cattacagag ctgttgtgct
    20461 gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg aataacggga
    20521 attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg tccgcctgta
    20581 atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg cggccaggga
    20641 acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg gttctgaatc
    20701 gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac acgtttttta
    20761 ggtttatcag catattgcgc tttcaattgc acgtactgat ccagcaggga ttgcgcggct
    20821 tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc attggcaatt
    20881 tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc cagctggtca
    20941 acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt cagcgcgaca
    21001 atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg cgcttgtgga
    21061 ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa ggcaagttca
    21121 gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag tggcgcgaga
    21181 aaagacaggg cgaccagcgc cctgaacagt gatttagcca tgtgccagtt tctgcaccag
    21241 tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat cgaagctaag
    21301 atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg ggacgttgaa
    21361 attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg cctgtgggaa
    21421 gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc cgctaacaat
    21481 caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg cagcgatcag
    21541 tttatcgtca gctttaaagc ctgtcggaca gcctggtaac tgaccgtatt cataaccaaa
    21601 tgccgtgaca tccgcgtcgt gataacgcgc ttcgtccgag acaacgatat cgcccacttt
    21661 cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg gcttgcagtg
    21721 ttccaacagc agagtggcac ccagcgccgc agcgacttta ccgatgcccg atttcagaag
    21781 cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccga gactgatagt
    21841 ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg caccaatgat
    21901 gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt taccatgcgc
    21961 ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat ttccgaaaaa
    22021 aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa catgagatcc
    22081 tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt cgtcgtctgc
    22141 aacaaaagac ccaggttttt ccactggagc gcaatgccgc cgtgcgcacg cgtcttaccc
    22201 actcgatgga agtccagcag gtgggacgct acatcgccaa agaaatttta agccgtctga
    22261 aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggcccc tttgaaagca
    22321 ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt cattttggcg
    22381 aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc gaaagccagc
    22441 ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg gaagaaccgc
    22501 ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg aatgcacaag
    22561 gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ttgggcacag gttggcggta
    22621 ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca catcactatt
    22681 taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg ttgcgtaaag
    22741 aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa gctgccgacg
    22801 acatctccta ttgtgtggca gaccttgaag atgcggtaga gaaaagaata tttaccgttg
    22861 agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt tcgctctttt
    22921 cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc cgcagtacgg
    22981 aagatcagtt ttttatgtat ttacgggtaa acaccctaaa caaactggta ccctacgcgg
    23041 cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat catgcattat
    23101 tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc gctgtaaaac
    23161 atgtatttag ccatccggat gtcgagcagc ttgaattgca gggctatcgg gtcattagcg
    23221 gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt actgaactgg
    23281 tagaaaaaga acgggtgaaa cgtttcccta ttgagtcgcg cttattccac aaactctcga
    23341 cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat tctcctgagt
    23401 ttccgctatg ggaatattat taccgttgcc gcctgctgca ggattatatc agcggtatga
    23461 ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa taaccaggct
    23521 tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg gaacttcggg
    23581 ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg agactgaaat
    23641 acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt ttggcgttat
    23701 ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag cagatgccaa
    23761 gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac gtagaaggta
    23821 gcacaaccgt taatacgccg cgtatgccgc gtaatttcca gcagttcttc ggtgatgatt
    23881 ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag gggggccagg
    23941 gcggtaatgg cggcggccag caacagaaat tcatggcgct gggttccggc gtcatcattg
    24001 atgccgataa aggctatgtc gtcaccaaca accacgttgt tgataacgcg acggtgatta
    24061 aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa gatccgcgct
    24121 ctgatatcgc gctgatccaa atccagaacc cgaaaaaact gaccgcaatt aagatggcgg
    24181 attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg tttggtctgg
    24241 gcgagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg aatgctgaaa
    24301 actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc ggtggtgcgc
    24361 tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca ccggacggcg
    24421 gcaacatcgg tatcggtttt gctatcccga gtaacatggt gaaaaacctg acctcgcaga
    24481 tggtggaata cgggcaggtg aaacgcggtg agctgggtat tatggggact gagctgaatt
    24541 ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta agccaggttc
    24601 tgcctaattc ctcggctgca aaagcgggca ttaaagcggg tgatgtgatc acctcactga
    24661 acggtaagcc gatcagcagc tttgccgcac tgcgtgctca ggtgggtact atgccggtgg
    24721 gcagcaaact gaccctgggc ttactgcgcg acggtaagca ggttaacgtg aacctggaac
    24781 tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc attgaaggcg
    24841 ctgagatgag caacaaaggt aaagatcagg gcgtggtagt gaacaacgtg aagacgggca
    24901 ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggtgcg aaccagcagg
    24961 cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct gtgctggcac
    25021 tgaacattca gcgcggcgac agcaccatct acctgttaat gcagtaatct ccctcaaccc
    25081 cttcctgaat acgggaaggg gttctcctta caatctgtga acttcgccac aactccatac
    25141 atcttcatca tcctttgggc atttgcacaa tgccgtacgt tacgtacttc cttatgctaa
    25201 gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat ggcgcaggat
    25261 atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga tgcccgcggg
    25321 cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg tgcattgctg
    25381 gtactttcac agggacgagt cgtcgatatc gatgacgcgg tagcacgtca tctgcacggt
    25441 gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg cgtaattggc
    25501 ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat gacggctgaa
    25561 atgatgctgg aacagtcgcg gttgatgcac ttgttggcgc aggatagccg tttgcgggaa
    25621 gaactggtga tgaacctgat tcaggcagag gagaatactc ccgcacttct gaatgggcgc
    25681 aacggctggg gatcgatctc aatcaaccgc gagtggtggc tattgttgag gtcgacagcg
    25741 gtcagcttgg cgtggacagc gcaatggcgg agttacaaca actgcaaaac gcgctgacta
    25801 cgcccgagcg taataatctg gtggcgattg tctcgctaac cgaaatggtg gtgttgaaac
    25861 cggcgttgaa ctcttttggg cgctgggatg cagaagatca tcgtaagcga gttgaacaac
    25921 tgattacccg catgaaagag tacggccagc tgcgttttcg cgtttcactg ggcaactatt
    25981 ttaccggtcc tagcagtatt gcccgatcct atcgtacggc gaaaacgacg atggtggtgg
    26041 gtaaacagcg gatgccagaa agtcgctgct atttttatca ggatctgatg ttacctgtgt
    26101 tactcgacag tttgcgtggc gactggcagg ccaacgaact ggcgcgaccg ctggcgcggc
    26161 tgaaagcgat ggacaataac ggcttgctgc gacgaacgct ggcggcgtgg tttcgccaca
    26221 atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca tcgtaatacc ctggagtatc
    26281 ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg caattttgat gacaggttgc
    26341 tgctgtatgt ggcgttacaa ctggatgaag agcggtagat tatgcgttaa ggttgaggcg
    26401 gcgctggctc attcccctca gcccgaaggg gagaggggaa tgcccgagtt aaccgtcaat
    26461 tcgggattat ttacgcgtta gtttttccag atcggcttca atctcgctga tcttattggt
    26521 tacgactgac tccaggtgac gtaagtcgtc gaggatctta cgcttaagat caacttcgct
    26581 gcggtcacgc tgacagattt gatcaagctc atcaatgata taccgtagat tcgggctgat
    26641 ttcctggact tctttataac cctgacccac accatcagcg acgaccgttt tacgctgacg
    26701 cggatattta aacttaacgc tcttggcgaa aaactcgcct ttgtctttct ggaaatagat
    26761 tttcagaata tcgttgttgg cttcctgccg gaggctgtaa cgatcaattt cttcaggatt
    26821 ggtaataccc agacttttca gattgtcgta catagcgtta cctcaaaatg agtcagtaaa
    26881 ttgtgcttat tttagcattt ggcttggccc gcccggctcg actgtgatcg cagaaagcct
    26941 ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa ttacctgctt tattgtggcg
    27001 atattccatg ggatggatta gcacatttat cttcgttttg cgaattacat gacagtccga
    27061 aacaagatga aaaagtattt aaaaaagggg gaggaagaat gagtaggacg aattattctg
    27121 agccagaaag catacgggca gcatcatgct gcccgtatat gtactcttaa tcgatggtac
    27181 gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc ttaacgataa
    27241 ccgcacagta gaggctgtat ttgccatctt ttgacggcag attacctgaa acaaccacag
    27301 accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa atacgggtgc
    27361 tctgaccaat gtatacgccc atagagatga cggaaccttc ttcgacaatc accccttcaa
    27421 ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat aatggtcggg ttagcctgca
    27481 gcggctccag cacgccgccg atgcccacgc caccggaaag gtggacgttt ttaccaatct
    27541 gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca acatatgcgc
    27601 cgatgttaac gtaagacggc atcagcacgg tgttacgggc aataaacgca ccctgacgta
    27661 ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg tcgtagtcgg
    27721 cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc acctgattat
    27781 cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc acccactgac
    27841 cgtcaatttt ttccgctaca cgcagtgcgc cggaatccag cagggcgatc acctgattta
    27901 ccgcttcgcg ggtaacggtg tctgcattgg ctggcgtgat ctcggcacgg cgttcaaaag
    27961 cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat cagtaaacac
    28021 atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact tcctgctgca
    28081 actcgttatt aagcgcacgc cggtcagcgg tggcaattat gaataaatct tctactcgct
    28141 cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca aaaattttcc
    28201 cgactcgcgc cagcagtcca ggttggtcga gggcgatcag ttcgaggaac gatttgcggt
    28261 cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt aatttggcgg
    28321 gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagcacctgc tccagaccaa
    28381 accgaataac ctcatggcga tctgcggaca gcgggctgcc atcgggttcc agcacgataa
    28441 aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga acacttaaat
    28501 tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc gggctccaga
    28561 taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc ggtttgctta
    28621 aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta tggcggacaa
    28681 aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg atgttatcca
    28741 tgcgcagtag tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc atatccggcg
    28801 tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg cgcaacagac
    28861 tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc actgtcaggc
    28921 ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc ttgatgactt
    28981 cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc tggcgaacca
    29041 gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca aaatgcacta
    29101 catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata tcgtgaaaca
    29161 gcgcggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc acacacaacg
    29221 gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc acgcggatag
    29281 tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg acgatatgcg
    29341 accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat agcccgcgcc
    29401 gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttgc ggaatattac
    29461 acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg gtggagtaaa
    29521 taccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg gcttccggct
    29581 ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc tgaaactcat
    29641 cgtcgagtgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca tcgaacagtt
    29701 gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa tccttcatca
    29761 tccgctcgac cggttcgttg ccttcaccac tgtaattcag acgctgagcg acgctaagtt
    29821 ggcgatcgaa taacagacga ttatcgtaac ggctgacgac cagatgcagg gcaaagcgaa
    29881 tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag gttaagaagc
    29941 caaacccgac catttcatcc agcgatgttg cgccaaaatg acggcgggcc acccattgca
    30001 gagtgtgaat atcgcgcaag ccgccagggc tacttttgat gtctggttca aggttgtagc
    30061 tggtgccatg gtaacgctga tggcgctggt tctgttcttc aactttcgct gcgtagaact
    30121 tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg aagagcgcaa
    30181 catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa tccgataacc
    30241 cttccaacat gcactcttca agcgtgcgca cactatgacc gacttccagc tttacatccc
    30301 agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc tttttacggc
    30361 ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg tagccaccga
    30421 cggcaaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat aatcgttgca
    30481 ggagctggtc tatgaactcg gtacgtgcct caatcaactg ttctgcagag atcccattgt
    30541 caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc ccacagacgg
    30601 ttaattcatc acggggccag gcgcatggat tttgcggttg accgggcagg gtggagagag
    30661 cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa gattaaatta
    30721 tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga gattatcgcc
    30781 gggatggtgt catccttgcg tagcgtcaaa atttcgcagc cgttatcagt caccacaata
    30841 gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca gccatctttc
    30901 atggtgcgga tctctttttt acccgcgttg accattggct caatggtgaa cgtcatcccc
    30961 ggtttcagca cgacgttggt ttcacgggag tcatagtgca gcacctgcgg ttcttcatgg
    31021 aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc ttctgcttcg
    31081 acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt taccatgcgt
    31141 agcgccaggt acaggctttc ttgcgtgatg cggcacaggc gttcgcccat gatggtcggc
    31201 ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat tacggtgaca
    31261 tcaatgttaa cgatatcgcc atctttcagc agcttagcat cgtccgggat accgtggcac
    31321 accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc gaggcaggca
    31381 gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag ctcgccggtg
    31441 ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc cagtcggcca
    31501 gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat taattctgtc
    31561 catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta tcacacccgg
    31621 gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaaggtttt ctataataga
    31681 aaattcgacg tctgatgctg tacacagcgc caacaattat tggtgtccac gacgtatttg
    31741 tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga cggaagcgac
    31801 aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg ccctttgggg
    31861 tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt tttaatcatg
    31921 gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca ccagacccgt
    31981 tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt tcacatcatc
    32041 aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa caagattgtt
    32101 tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga agcggtgaaa
    32161 gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg cggtatgctg
    32221 actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga aactcagtct
    32281 caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac tcgtgagctg
    32341 gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc ggacgctctg
    32401 tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa cttgggtatt
    32461 ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt cgttatcccg
    32521 ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc tgcaaccgta
    32581 cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt agaagctgag
    32641 taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg caagctcgag
    32701 aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac aacgcatctg
    32761 cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt ttggttaggg
    32821 ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc cgggcagatc
    32881 acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa gagctgcgtg
    32941 agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct aacggcgaca
    33001 tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg aaaaaagcag
    33061 gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac ggcatcattc
    33121 tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag gcgttcgcag
    33181 acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt ctgaaagcac
    33241 agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgaaaacatc aacattcgcc
    33301 gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg cgtatcggcg
    33361 ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct atgcacgttg
    33421 ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg gtagaaaaag
    33481 aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa atcgcagaga
    33541 aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc ggtcagccgt
    33601 tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac gctgaagtga
    33661 ctggcttcat ccgtttcgaa gtgggtgaag gcatcgagaa agttgagact gactttgcag
    33721 cagaagttgc tgcgatgtcc aagcagtctt aattatcaaa aaggagccgc ctgagggcgg
    33781 cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg ctgaaaagcg
    33841 acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg aaagaaacat
    33901 ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg gcgaagctct
    33961 gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg ctcaggaaat
    34021 caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg gtaacctgtt
    34081 ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc acatggggat
    34141 gctggcgacc gtaatgaacg gcttggcaat gcgtgatgca ctgcaccgcg cctatgtgaa
    34201 cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca gctgggcaga
    34261 agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta caggtaaccc
    34321 gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag ccgatgtggt
    34381 gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag atccaaccgc
    34441 aaccatgtac gagcaattga cttacagcga agtgctggaa aaagagctga aagtcatgga
    34501 cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt tcaatatgaa
    34561 caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt taatcacgga
    34621 ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg ggggcgtaag
    34681 tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg cagcaaccgg
    34741 agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg caaataaaat
    34801 agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac ttagcacact
    34861 tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc gtaacgtgat
    34921 tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag cgttcaaaac
    34981 ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg gcattgtcgt
    35041 ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg tagaagattc
    35101 ccgtactctg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg aaaaagcgat
    35161 tatggcctcc gatctcggcc tgaacccgaa ctctgcgggt agcgacatcc gtgttccgct
    35221 gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg gtgaagcaga
    35281 acaagcgcgt gttgcagtac gtaatgtgcg tcgtgacgcg aacgacaaag tgaaagcact
    35341 gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg atgtacagaa
    35401 actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag aagcagaact
    35461 gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt gcggatcggc
    35521 tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag caactcacca
    35581 ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc cataatcccg
    35641 aacacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg gtagaacagt
    35701 gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg aaacttctta
    35761 aaacgatgct acagcaacag gggagccgca ccgaagtctt aagtgggcaa caagccgctt
    35821 gcgatatggc agcgcttgag gaggttgatc aggtgatggc agccattgtt ggtgctgctg
    35881 ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg gccaataaag
    35941 aatcactggt tacctgcgga cgtctgttta tggacgccgt aaagcagagc aaagcgcaat
    36001 tgttaccggt cgatagcgaa cataacgcta tttttcagag tttaccgcaa cctatccagc
    36061 ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta cttaccgggt
    36121 ctggtggccc tttccgtgag acgccattgc gcgatttggc aacaatgacg cccgatcaag
    36181 cttgccgtca tccgaactgg tcgatgggac gtaaaatttc tgtcgattcg gctaccatga
    36241 tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc gccagccaga
    36301 tggaagtgct gattcacccg cagtcagtga ttcactcaat ggtgcgctat caggacggca
    36361 gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac accatggcat
    36421 ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta agtgcgttga
    36481 catttgccgc accggattat gagcgttatc catgcctgaa actggcgatg gaggcgttcg
    36541 aacaaggcca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc gttgctgctt
    36601 ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatct gtactggaaa
    36661 aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat gcgaacgcga
    36721 gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc ggctacagag
    36781 agtcgcgcta tttgttagcg tagggcttca atgatatagt ctgcgccatc tgatcgtaag
    36841 tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt tgtataggct
    36901 tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga tgttgtctgc
    36961 tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg cgatcattat
    37021 ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg ggcataaagc
    37081 cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta ttgaagcgtt
    37141 aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag tcagtgcgtt
    37201 aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc gacataacgt
    37261 gcgtctgcgt attattggcg ataccagtcg ttttaactcg cgtttgcaag aacgtattcg
    37321 taaatctgaa gcgctaacag ccggaaatac cggtctgacg ctgaatattg cggcgaacta
    37381 cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg tgcagcaagg
    37441 aaacctgcaa ccagatcaga tagatgaaga gatgttaaac cagcatgtct gtatgcatga
    37501 actggcccct gtagatttag taattaggac tgggggagag catcgcatta gtaacttttt
    37561 gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc ccgatttcga
    37621 tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc gtttcggcgg
    37681 caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt atcgcctgat
    37741 atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc cgccggtggg
    37801 gttcgccatt gtaacgctgg tggtctgcat gctggcagcg tgggaatggg gacagcttag
    37861 cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt tattgttggc
    37921 gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc tggttgaaat
    37981 ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc tgttttaccc
    38041 aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg gcgtgctaac
    38101 cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactatg acgagaatca
    38161 ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggtgcg ctgactccgg
    38221 cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg tttctccggg
    38281 taaaacctgg caaggcttta taggtggact cgctactgca gcggtaatct catggggtta
    38341 tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct ctattgtcgc
    38401 agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg aagcaggaat
    38461 taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta ttgatagcct
    38521 gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga cgctttaacg
    38581 gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc actgggtgta
    38641 cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg tgttcgcgtt
    38701 gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa gctcggcacc
    38761 gaatatgtta tcgccctgat cccgttgggc ggttatgtca aaatgctgga tgagcgcgca
    38821 gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt cggccaacga
    38881 gtggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt tgcctactgg
    38941 ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggcgaaat agcagccaat
    39001 tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt agatggtatc
    39061 gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg cgatgaaagc
    39121 accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa gctcgattta
    39181 cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg gattcgtcct
    39241 cgtgggccgc aaattgaacc tgtactggaa aatgtgcagc caaactcggc ggcaagcaag
    39301 gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt aacgcagtgg
    39361 gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt agaaatcgaa
    39421 aggcagggga gtcccttgtc tttgacatta atcccggaga gtaaaccggg taatggtaaa
    39481 gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga gtataaagtt
    39541 gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac gtggcagctg
    39601 atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa actgaacaac
    39661 ctcagtgggc cgatttctat cgccaagggg gctgggatga cagcggaact cggggtagtt
    39721 tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa cctgtttccg
    39781 ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat caagggcgga
    39841 ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct gctggtgctg
    39901 ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt taggaagaac
    39961 gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt agcagcgcca
    40021 ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc cttcagcgtg
    40081 tcgccgttgg tgcggccctc ctcagtatgc cggtgcgtac aggcgacacg gttaatgatg
    40141 aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag gatgttcgcg
    40201 tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc attgccagca
    40261 ttactttctc cggtaacaaa tcggtgaaag atgacatgct aaagcaaaac ctcgaggctt
    40321 ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc gagaaaggtc
    40381 tggaagactt ctactacagc gtcggtaaat atagcgccag cgttaaagct gtcgtgaccc
    40441 cgctgccgcg caaccgtgtt gacctaaaac tggtgttccg ggaaggtgtg tcagctgaaa
    40501 tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg atctctcatt
    40561 tccaactgcg tgacgaagtg ccgtggtgga atgtggtagg cgatcgtaaa taccagaaac
    40621 agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggaccgc ggttatgccc
    40681 gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt atttacgtca
    40741 cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg agcggcaacc
    40801 ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggc gagctgtata
    40861 acggcactaa agtgaccaaa atggaagatg acattaaaaa gcttctcggt cgctatggtt
    40921 atgcctatcc gcgcgtacag tcgatgcctg aaattaacga tgccgacaaa accgttaaat
    40981 tacgcgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt tttgaaggta
    41041 acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa ggtgcatggt
    41101 tggggagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc ttctttgaaa
    41161 ctgtcgatac tgatacccaa cgtgttccgg gtagcccgga ccaggttgat gtagtctaca
    41221 aggtgaaaga acgtaacact ggtagcttca acttcgggat tggttacggt actgaaagtg
    41281 gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt tatgctgttg
    41341 gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta accaacccgt
    41401 acttcaccgt agatggcgta agcctcggtg gtcgtctctt ctataatgac ttccaggcag
    41461 atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg acgttgggct
    41521 tcccgattaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat aactccctgt
    41581 ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt gaacatccga
    41641 gcacttctga tcaggataac agcttcaaaa cggacgactt cacgttcaac tatggttgga
    41701 cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc aacctgaccg
    41761 gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta gacacggcga
    41821 cttatgtgcc gatcgatgac gatcacaaat gggttgttct gggtcgtacc cgctggggtt
    41881 atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat gccggtggtt
    41941 ccagcaccgt gcgtggcttc cagtccaata ccattggtcc gaaagcagtt tacttcccgc
    42001 atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag gacggcgcga
    42061 aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt gccagcctcg
    42121 agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt cgtacttcat
    42181 tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa tattctggat
    42241 atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca ttacaatgga
    42301 tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag tacgatggag
    42361 acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg
    42421 aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc gggccacgca
    42481 aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa aaagtggtta
    42541 ttagctgcag gtcttggttt agcactggca acttctgctc aggcggctga caaaattgca
    42601 atcgtcaata tgggcagcct gttccagcag gtagcgcaga aaaccggtgt ttctaacacg
    42661 ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac cgatctgcag
    42721 gctaaaatga aaaaactgca gtccatgaaa gcgggcagcg atcgcaccaa actggaaaaa
    42781 gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga gcaggatcgc
    42841 gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac tgctgtgaaa
    42901 tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgctgt tgcttacaac
    42961 agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata agtaatgcct
    43021 tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg tgatggcgat
    43081 atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat tacgttcatg
    43141 gttaacccaa aataccgtga gcatttaggc ttgtgccaag cgtccgcggt tgtcatgacc
    43201 caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc ctacctgact
    43261 tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa cattgcaccc
    43321 agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg cgctaacgcg
    43381 gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg ttgcttcgta
    43441 ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac catttaccat
    43501 gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg cgcagacggc
    43561 tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg tcgcgtaatt
    43621 attggcgatc gagtggagat cggtgcctgc acaaccatcg atcgcggcgc gctggatgac
    43681 actgttattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca taacgtcgtg
    43741 attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct gaaaattggt
    43801 cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat atgcgacaaa
    43861 gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg cgtctattcc
    43921 tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact ggtgatgaac
    43981 attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca acaagactaa
    44041 cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat tgcggcaggc
    44101 cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact actaacactc
    44161 atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg ttcttactgg
    44221 tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa aatgtctctg
    44281 tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg ggtgtgctga
    44341 ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta ggaaaactgg
    44401 aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag cgcccggtcg
    44461 tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc ggcctgaccc
    44521 gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg atgatgtgtg
    44581 ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat ccaaccgcca
    44641 ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt tgtatcgttg
    44701 gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc gtgaatggtc
    44761 atactaaaat tggccgcgat aatgagattt atcagtttgc ctccatcggc gaagttaacc
    44821 aggatttgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt aaccgcattc
    44881 gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg aaggtgggca
    44941 gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta ggtaaccgct
    45001 gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac ttcgcgatca
    45061 tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcatgtg atggttggcg
    45121 gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt aaccacgcaa
    45181 cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt gaggcgatta
    45241 ccgctatccg caatgcgtat aagctaattt atcgtagcgg taaaacgctc gatgaagtga
    45301 aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt accgatttct
    45361 ttgcacgctc aacgcgcggt ctgattcgtt aatgactgac cagcgtccat taacgattgc
    45421 cctggtcgcc ggagaaatct ccggcgatat cctgggggcc ggtttaatcc gcgctctgaa
    45481 agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc aggctgaagg
    45541 ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg aagtgctcgg
    45601 tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg gcgaactgaa
    45661 gccagatgtt tttgttggta ttgatgcgcc cgacttcaat attactcttg aaggtaacct
    45721 caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg cgtggcgaca
    45781 gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc tgcctttcga
    45841 aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata ccatggctga
    45901 tgccatgccg ttagatccag ataaaaatgg tgcccgtgat gtgctgggga tcccttacga
    45961 tgcccactgt ctggcattgt tgccgggcag ccgtggtgca gaagtcgaaa tgcttagtgc
    46021 cgatttcttg aaaactgccc agcttttgcg ccagacatat ccggatctcg aaatcgtggt
    46081 gccgctggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg aagtcgcgcc
    46141 agacctgtcg gttcatttgc tggatggaat gggccgtgag gcgatggtcg ccagcgatgc
    46201 ggcactactg gcgtcgggga cggcagccct ggagtgtatg ctggcgaaat gcccgatggt
    46261 ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg tgaaaactga
    46321 ttatgtctcg ctgccaaatc tgctggcggg cagagagtta gtcaaagagt tattgcagga
    46381 agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga acgggaaaac
    46441 cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct gcaatgccga
    46501 tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt ttatccgcac
    46561 acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg cgcggtcgtc
    46621 accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga ttccaaaaag
    46681 ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc gttgagctgg
    46741 agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca tgcgaccatg
    46801 ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt gttgattgat
    46861 ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg ggatagccgc
    46921 gtaccagaaa tcagtgccgc gtctatccta gcgaaagtga cgcgtgacgc cgaaatggcg
    46981 gcgctggata ttgttttccc gcaatatggt tttgcccaac ataaagggta tccaaccgct
    47041 tttcatctgg aaaaactggc tgaatatggc gcgactgaac atcatcgtcg cagctttggg
    47101 cctgtcaaac gcgcactggg acttgcgtcc tgattcttgt gtcgagatta agtaaaccgg
    47161 aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga ctactcgatg
    47221 atcgatggct tggccaaaac cgcaccgctg gtaaaaaagg cggcggcgtt gggtatgcca
    47281 gcactggcga tcaccgattt caccaacctt tgcggtctgg tgaagttcta cggagcggga
    47341 catggcgcag ggattaagcc tatcgtcggg gcagatttta atgtccagtg cgacctgctg
    47401 ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta tcagaatctg
    47461 gcgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc gatcatcgat
    47521 cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg gcgcatgggc
    47581 gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg tgtcgcgttt
    47641 tatgaagaac acttcccgga tcgctatttt ctagagctga tccgcaccgg caggccggat
    47701 gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt gcccgtcgtg
    47761 gcgactaacg atgtgcgctt tatcgacagc agcgactttg acgcacacga aatccgcgtc
    47821 gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta ttcgccgcag
    47881 caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc agaagccctt
    47941 gccaacaccg ttgagatcgc caaacgctgt aacgtaactg tgcgtcttgg tgaatacttc
    48001 ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa gcgtgcaaaa
    48061 gaaggcctgg aagagcgtct ggcattttta ttccctgacg aggaagaacg tcttaaacgc
    48121 cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca gatgggtttc
    48181 ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa cggcgtaccg
    48241 gtaggaccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct gaaaatcacc
    48301 gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc ggaacgtgtc
    48361 tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt tatcgaacac
    48421 gtggcggaca tgtacggtcg tgatgcggta tcgcagatta tcaccttcgg tacgatggcg
    48481 gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg ctttgtcgac
    48541 cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc gtttgaagcc
    48601 gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct gatcgacatg
    48661 gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg ggtggttatc
    48721 gcgccgacca aaattaccga ttttgcgccg ctttattgcg atgaagaggg caaacatccg
    48781 gtcacccagt ttgataaaag cgacgttgaa tacgccggac tggtgaagtt cgacttcctt
    48841 ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa gcggcgggcg
    48901 aagaatggcg agccaccgct ggatatcgcc gcgatcccgc tggatgacaa gaaaagcttc
    48961 gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg cggcatgaag
    49021 gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact ggtggcactg
    49081 ttccgccctg gtccgttgca gtcagggatg gtggataact ttatcgaccg taaacatggt
    49141 cgtgaagaga tctcctatcc ggacgtgcag tggcagcatg aaagcctgaa atcggtactg
    49201 gagccaacct acggcattat cttgtatcag gaacaggtca tgcagattgc ccaggtgctt
    49261 tctggttata ccctcggtgg cgcggatatg ctgcgccgtg cgatgggtaa gaaaaagccg
    49321 gaagagatgg ctaagcagcg ttctgtattt gctgaaggtg cagaaaagaa cggaatcaac
    49381 gccgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgcgggtta cggatttaac
    49441 aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct gaaagcgc
//
LOCUS       DADLTF010000012        44108 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.012, whole genome shotgun sequence.
ACCESSION   DADLTF010000012 DADLTF010000000
VERSION     DADLTF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.012"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1305..2987)
                     /gene="glcA"
                     /locus_tag="JN749_000687"
     CDS             complement(1305..2987)
                     /gene="glcA"
                     /locus_tag="JN749_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HAY7367199.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFAGVIGVITLLQAYVFTGMLVS"
     gene            complement(3342..5513)
                     /gene="glcB"
                     /locus_tag="JN749_000688"
     CDS             complement(3342..5513)
                     /gene="glcB"
                     /locus_tag="JN749_000688"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HAY7367200.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGIVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5535..5939)
                     /locus_tag="JN749_000689"
     CDS             complement(5535..5939)
                     /locus_tag="JN749_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HAY7367201.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5944..7167)
                     /gene="glcF"
                     /locus_tag="JN749_000690"
     CDS             complement(5944..7167)
                     /gene="glcF"
                     /locus_tag="JN749_000690"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HAY7367202.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(7178..8230)
                     /gene="glcE"
                     /locus_tag="JN749_000691"
     CDS             complement(7178..8230)
                     /gene="glcE"
                     /locus_tag="JN749_000691"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HAY7367203.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFCYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8230..9729)
                     /gene="glcD"
                     /locus_tag="JN749_000692"
     CDS             complement(8230..9729)
                     /gene="glcD"
                     /locus_tag="JN749_000692"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HAY7367204.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANELGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9975..10745
                     /gene="glcC"
                     /locus_tag="JN749_000693"
     CDS             9975..10745
                     /gene="glcC"
                     /locus_tag="JN749_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HAY7367205.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10965..12011)
                     /locus_tag="JN749_000694"
     CDS             complement(10965..12011)
                     /locus_tag="JN749_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7367206.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQDFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEM"
     gene            12415..14103
                     /locus_tag="JN749_000695"
                     /pseudo
     CDS             12415..14103
                     /locus_tag="JN749_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14118..15014
                     /locus_tag="JN749_000696"
     CDS             14118..15014
                     /locus_tag="JN749_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAY7367207.1"
                     /translation="MTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNLTWVRGS
                     LEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGFCQRFLF
                     ISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKPLFDWML
                     RGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDWQRVRQL
                     AAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADWSASNNR
                     ISEDINWFPGISLEHALRNGLF"
     gene            15047..15295
                     /locus_tag="JN749_000697"
     CDS             15047..15295
                     /locus_tag="JN749_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7367208.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15295..16467
                     /locus_tag="JN749_000698"
     CDS             15295..16467
                     /locus_tag="JN749_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAY7367209.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16503..17582)
                     /gene="lptG"
                     /locus_tag="JN749_000699"
     CDS             complement(16503..17582)
                     /gene="lptG"
                     /locus_tag="JN749_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7367210.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17579..18649)
                     /gene="lptF"
                     /locus_tag="JN749_000700"
     CDS             complement(17579..18649)
                     /gene="lptF"
                     /locus_tag="JN749_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7367211.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPWQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18680..19240)
                     /locus_tag="JN749_000701"
     CDS             complement(18680..19240)
                     /locus_tag="JN749_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7367212.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLSNE"
     gene            complement(19252..20076)
                     /locus_tag="JN749_000702"
     CDS             complement(19252..20076)
                     /locus_tag="JN749_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367213.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20076..21425)
                     /locus_tag="JN749_000703"
     CDS             complement(20076..21425)
                     /locus_tag="JN749_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAY7367214.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21471..22229)
                     /locus_tag="JN749_000704"
     CDS             complement(21471..22229)
                     /locus_tag="JN749_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367215.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22261..22974)
                     /locus_tag="JN749_000705"
     CDS             complement(22261..22974)
                     /locus_tag="JN749_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7367216.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23148..23840
                     /locus_tag="JN749_000706"
     CDS             23148..23840
                     /locus_tag="JN749_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HAY7367217.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23889..25388)
                     /gene="pitB"
                     /locus_tag="JN749_000707"
     CDS             complement(23889..25388)
                     /gene="pitB"
                     /locus_tag="JN749_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HAY7367218.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25680..27539)
                     /gene="gss"
                     /locus_tag="JN749_000708"
     CDS             complement(25680..27539)
                     /gene="gss"
                     /locus_tag="JN749_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HAY7367219.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27744..28610
                     /gene="yghU"
                     /locus_tag="JN749_000709"
     CDS             27744..28610
                     /gene="yghU"
                     /locus_tag="JN749_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HAY7367220.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28733..28981)
                     /gene="hybG"
                     /locus_tag="JN749_000710"
     CDS             complement(28733..28981)
                     /gene="hybG"
                     /locus_tag="JN749_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HAY7367221.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29115..29456)
                     /gene="hypA"
                     /locus_tag="JN749_000711"
     CDS             complement(29115..29456)
                     /gene="hypA"
                     /locus_tag="JN749_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7367222.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29449..29937)
                     /gene="hybE"
                     /locus_tag="JN749_000712"
     CDS             complement(29449..29937)
                     /gene="hybE"
                     /locus_tag="JN749_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HAY7367223.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29930..30424)
                     /locus_tag="JN749_000713"
     CDS             complement(29930..30424)
                     /locus_tag="JN749_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HAY7367224.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLSDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30424..32127)
                     /gene="hybC"
                     /locus_tag="JN749_000714"
     CDS             complement(30424..32127)
                     /gene="hybC"
                     /locus_tag="JN749_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HAY7367225.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32124..33302)
                     /gene="hybB"
                     /locus_tag="JN749_000715"
     CDS             complement(32124..33302)
                     /gene="hybB"
                     /locus_tag="JN749_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HAY7367226.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33292..34278)
                     /gene="hybA"
                     /locus_tag="JN749_000716"
     CDS             complement(33292..34278)
                     /gene="hybA"
                     /locus_tag="JN749_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HAY7367227.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34281..35399)
                     /gene="hybO"
                     /locus_tag="JN749_000717"
     CDS             complement(34281..35399)
                     /gene="hybO"
                     /locus_tag="JN749_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HAY7367228.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35588..35875)
                     /locus_tag="JN749_000718"
     CDS             complement(35588..35875)
                     /locus_tag="JN749_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HAY7367229.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35994..36920)
                     /locus_tag="JN749_000719"
     CDS             complement(35994..36920)
                     /locus_tag="JN749_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708817.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7367230.1"
                     /translation="MLCLKKHQLRNATMPRLTAKDFPQELLDYYDYYVHGKISKREFL
                     NLAAKYAVGGMTALALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLV
                     KPAKMSGKTPAVGVVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKG
                     RELQQQVDPTKLMNDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACA
                     VPFYGRQAPTADVAKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVN
                     HGFHNDSTPRYDKSAADLAWQRTLKWFDKYLS"
     gene            37038..38078
                     /gene="gpr"
                     /locus_tag="JN749_000720"
     CDS             37038..38078
                     /gene="gpr"
                     /locus_tag="JN749_000720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAY7367231.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38118..38612)
                     /locus_tag="JN749_000721"
     CDS             complement(38118..38612)
                     /locus_tag="JN749_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HAY7367232.1"
                     /translation="MERFFENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38803..39688
                     /gene="yghA"
                     /locus_tag="JN749_000722"
                     /pseudo
     CDS             38803..39688
                     /gene="yghA"
                     /locus_tag="JN749_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
     gene            complement(39960..40385)
                     /gene="exbD"
                     /locus_tag="JN749_000723"
     CDS             complement(39960..40385)
                     /gene="exbD"
                     /locus_tag="JN749_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HAY7367233.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40392..41126)
                     /locus_tag="JN749_000724"
     CDS             complement(40392..41126)
                     /locus_tag="JN749_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HAY7367234.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41378..42565
                     /gene="metC"
                     /locus_tag="JN749_000725"
     CDS             41378..42565
                     /gene="metC"
                     /locus_tag="JN749_000725"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HAY7367235.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42705..43364
                     /locus_tag="JN749_000726"
     CDS             42705..43364
                     /locus_tag="JN749_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7367236.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43404..>44108)
                     /locus_tag="JN749_000727"
     CDS             complement(43404..>44108)
                     /locus_tag="JN749_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7367237.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10941 a        11762 c        10838 g        10567 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagctcgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttaatga ttttttcatt tcttcgcgcc taataaaagt attcaaattc agatgatttt
      601 tctgctcaac agacacaaac aggcaataat actctccaaa gcgaacaata tttggcatta
      661 cgatttaatg cttcataatc accaaattat ttaaccataa attttcttat ttctaacaaa
      721 atgattggtg tgttgttgga gttaaaatac atacataata ttaacatctg ccaacatttc
      781 actttccgca agacggatga tttcgaatcc tcatccacgt taccctatcc caaatatctt
      841 tattaatagg cagtaacgaa cactgttata gcaagattaa cttattaacc taacggcatc
      901 ttttatgtaa caagtggaga taaattgctt aaaattgatc ctcgacaaat cttaaaagtt
      961 ctcattattc tgcaaaatca gctttgcgaa taatcttatg tttttgggag ttttgggaat
     1021 tttgatctac tcattgttct agtgaaaaat ataaataaga gaaaaaacgc cattcgtcat
     1081 gaaagcaaca gatgaagcaa tgtaataaca tactaaaatt gatctcatga aagtgggtac
     1141 tttataacca gcacagaagc caagttgtat aaatattaaa caattcgcat cgtgaaacgg
     1201 agactacgta taaagaagcc aatcgtaaaa acggttccct ggcgagataa acggcagaca
     1261 aatggaaagc cgggtgatat ttaccttcat ccggctttat tgatttacga gactaacatc
     1321 ccggtaaaca catacgcctg cagcagggtg ataacgccga taacgccggc aaaaatcaga
     1381 ctgtgcttca cggtgtagcg gaacagttca gattctcggc ccaccatgcc cgtcgcggcg
     1441 caggccacgg cgatagattg cggggagatc atcttgccag ttacgccgcc gctggtattt
     1501 gctgccacca gcagagtgtc agagacgttg atttgctgcg ccgtggtcga ttgcagtgaa
     1561 ccaaacaggg cgtttgaaga agtgtccgac ccggtaagga atacgcccag ccagccgaga
     1621 aacggtgaga aaaacgggaa catcacgcct gtacctgcca gtaccagcgc cagcgtggtg
     1681 gacatgccgg aatagttggt gacgaaggcg aatgccagca ccatgccaat cgacaatatc
     1741 ggccacttca gactaattag cgtttcggca aagacgccaa tccctttctt gatgcccacg
     1801 ccgaggatga agatagagat aatcgccgca ataaaaatag cggtgccgcc agccgagaga
     1861 ggttcgaatt taaacaccgc atccattggc gttggttggg cgacaatggg tgccgctttc
     1921 aacacttgtt gatgcaaatg agggatctgg aaattaatca ccagtgaata aaacgcgccg
     1981 cccggagcaa ataacgcttt aaacggcttc atggtccaga tggtgaccag caccgttaag
     2041 attaaaaacg gtgaccacgc acgaatgatt tgccccagac tatattctga aggcacggga
     2101 ccgccagaag atggcttatt taccaccatc gcacctgcgg attgccccat gctgattgcc
     2161 gtttcggtat ttttcggccg ccagacttta aggaataaag cgagtgagac gatactcacc
     2221 agcgccgaag taatatccgg cagttccgga ccaatatagt tagaggtaaa gaactgagtg
     2281 acagcgaagc ttcccccagc aaccagcgcc gctggccacg tctctttcac ccctttccag
     2341 ccgtccatca ttgctaccag ccagaacggc acaagaaccg acaggaacgg taactgacgt
     2401 cccgccattg cgccaatgtg gaacggatcg attcccgtta cctgaccggc gaccagaatc
     2461 ggcacgccca acgcaccaaa cgccaccggc gcagtattgg caatcagaca cagccccgcc
     2521 gcgtataacg gtttgaagcc caggcccacc agcagcgcac cggtaatcgc caccggcgca
     2581 ccaaagccag ccgctccttc cagcaacgca ccaaaggaga aaccaatcag taacacctgc
     2641 aaacgctgat cgtcggtgat ggagataacc gagctgcgga taatatcgaa ctgcccgctg
     2701 gcaacggtta atttatacag gaacaccgcc gcgacaataa tccacgcaat tggccataat
     2761 ccataaataa agccatagcc cgcagcagca aatgccatat caatcggcat tttaaaggcg
     2821 aatatcgcaa tcaggataga taatataagg gttattgctc cagcgacatg tcctttcaga
     2881 cgtaataccg cgagtgcaac gaagaagaat attatcggga tcagggcgac cagagcggat
     2941 agccccagtc ctcccatcgg catatacatt tgggtccagg taaccatatt gttcagtctc
     3001 ttattatctt tatatgcttg atatacttaa ggttgtaata agcaaaagag gactgaactg
     3061 taaaatatag gcgttatact ttacagcaac agtacgccgc taacgcaatt gctacctctg
     3121 gcataacaag tatatcgggt aagggtttct gttccgcaca cgcaggcgca gagtatcgtt
     3181 aagatgtcca tattgttgtt ttaggcccgc tagtaatgcg ctacgggtat ttaatattgt
     3241 taaaccctga taatcgctcc ggttatttcc gggataaatg tactaccgca gttactatca
     3301 tagccccgac aataaaactt gccggggctt ttttgacgct attaatgact ttctttttcg
     3361 cgtaaacgcc aggcgtgtaa taacggttcg gtataaccgt ttggctgttt cacgccgagg
     3421 aagattaaat cgctggcagc tttaaaagca cacgagttag cgaaattccc cgccatcgga
     3481 cgataagccg gatcgccagc attttgctga tcaaccactt tcgccatatt ctccagcgac
     3541 gcctgcacct gttctttggt cagaataccg tgacgtaacc agttggcgat atgctggctg
     3601 gagatacgca gcgttgcgcg gtcttccatc agcgccacgt tgtgaatatc cggcactttt
     3661 gaacaaccaa tcccctgctc cacccaacgc accacgtacc ccagaatccc ctgcacgttg
     3721 ttatccagct cttgttggat ctcttgcgcc gaccagttag cgttttcagc caccggaata
     3781 gtcagcagat cgtccagcag cggttcaaat tcagcattga actcggtctg ggcaatgttg
     3841 gcttgcacgc tctgtacgtt ggtttggtgg tagtgcagcg catggagcgt agcagcggtt
     3901 ggtgacggaa cccaggctgt gtttgccccg gcacgcagtt ggtcgccctt ctggctgtac
     3961 atgtctgcca tcaggtccgg cattgcccac atgcctttac cgatttgcgc tttaccgcgc
     4021 agcccacaga acagaccgga aagcacgtta ttacgttcgt aggctttgat ccacggcgtc
     4081 gatttcatct ggtttttacg cagcatcggg ccagcttcca tcaccgaatg catttcatcg
     4141 ccggtacggt cgaggaaacc ggtattgatg aacgccacgc ggttgcgcgc ctgagcgata
     4201 cagctccgca agttcagcga ggtccggcgt tcttcatcca taatgcccat tttcagggta
     4261 ttcggtacta taccgagcat tgtctcaatg cgggtaaaca gtttgttggc gaacgccact
     4321 tcctgcggac cgtgcatttt cggtttcaca atatagacgc tgccagtgcg cgagtttttc
     4381 tgcactttta aatcatagag ggcaatcgcg ccagtcatga cgccatcaag aataccttcc
     4441 gggatttcat tgccttcgct gtcccaaatc acaggaatgg tcatcaaatg acccacgttg
     4501 cggataaaca gcaacgagcg tccgtgcaga gaaatttcag agccatcggc ggcggtgtac
     4561 tgacgatcgt cattcagttt acgtacgatt tgccgaccgt ttttctccat tttctcttgc
     4621 agagtcccct gcatcaggcc cagcagattg cggtacagca ggattttatc ttccgcatca
     4681 accgccgcga ccgaatcttc gcagtcgaga atggtactga tagcagcttc gacgataaca
     4741 tcgttgatgt gcgccggatc gtctttgcca atccgcccgt tggcatcaat ttgcagttca
     4801 atatgcaggc cgttattttt cagcaaaatg caggtcggcg cagcggcatc gccacggtaa
     4861 ccgacaaact gtgctggagt acgtaacgtg gtttctttac catttttcaa ctggatgcgt
     4921 aattgtttat cgaccacctt aaacgccacc acatcctgat agctgccgtt ttccagcggt
     4981 agtgattcat cgaggaaacg tcgaacccag gcgataacct gctcaccgcg ctgcggatcg
     5041 tagccactga ccatcgcccc ttcctgcggg atgatgtcgc tgccgtataa cgcatcgtac
     5101 agtgagcccc agcgagcgtt cgccgcgttc agcgcgtagc gggcgttcat tgccggaacc
     5161 accagctgtg gccccgcctg actggtgatt tcgctgtcaa tgcccgtggt ttccaccgtc
     5221 acgcgctccg gttgcggcac caggtagccc agttcacgaa ggaaagattt ataggccgct
     5281 ttatctttta ccggccccgg attgctgcga tgccactcat caagcgccgc ctgaatgcga
     5341 tcgcgttctg ccagcaactg acgattttct ggtgccagat cgtgaacgat ctcatcaaca
     5401 ttgtgccaga atgcagcagc gtccagttcc acgcccggca aaacttctcc atccacaaaa
     5461 cgtttaaaat tggcgtcaat gcgtaaacgg ccctgggtta tggtttgact cattgtttct
     5521 ctcctcgttt tcgcttattt cgccaacacc gctgctgccg ctttcgcgac ctgcgcatcc
     5581 tgtgctccgg ttaaaccaga aacgcccacg gcaccaataa tttgcccatc aacaacaacc
     5641 ggtacgccgc cttccagcga cgtcagtaac ggcgcagtca cgaacgcggt acgtccgttg
     5701 ttcaccatct cttcatagcc cttagtttca cgacgcccca gcgcggcggt acgcgctttc
     5761 tcctgggaga tgtaagccgc aatcggcgcg caatcgtcca tgcgacttaa cgccagcaga
     5821 tgaccgccgt catcggcaac agcaatggaa acagaccagt tatttttctg cgcctcttcc
     5881 tgacctgcgg caataattgc actcgccatt tgctggctaa gaatgacttt agttttcatt
     5941 tttttattcc ttttcaaggg cttgttctac aatttcaatc cagtgacgca cagaggtacg
     6001 accggcgctc gccagatgcg tctggcaacc aatgttggcg gtgacgatca tttccggttt
     6061 gccgctttcc agcgcattca ttttgttatc ccgcagctgg cgtgccagat cgggatgcgt
     6121 taacgcatat gttcccgctg aaccgcagca cagatggctg tcgggaacgt ccgttaaggt
     6181 aaatccaaga cgaagtaaca cgttttccac ttcgccgttc agcttttgcg catgttgtag
     6241 ggtacacggg cagtggaagg ccagtttttt atcgccgcga attgccagtt tttccagcgg
     6301 ttcctcgcgc agaagttcga ctaaatcgac cgccagttca ctgacctgac gcgctttatc
     6361 ggcatataac gcatcgtttt tcagcatctg cccatactct ttgacgaacg cgccgcaacc
     6421 gctggcggtt tgcaaaattg cctcggcacc tgcttcaatc gcgggccacc aggcatcaat
     6481 attattgcgc gcccgtgcca accctttctc ctgcgcatta agatgatagt ccaccgcgcc
     6541 acaacagcct gcttcgttag ctgacatgac gctgatcccc agacgatcca gcactcgcgc
     6601 agttgccgcg ttggtgttgg gcgaaagcgt aggctgggcg cagccttcca gcattaaaac
     6661 ccgacgctta tggcgcagcg gcggacgcgg tttagctttc accgtttcag caggcagttt
     6721 tgctctgacc tgttccggta aaaacggtcg cagcaccagc cctacctgcg tcagcgcacg
     6781 gaagaccgcc ggacgcggca ctacctggcg caatccttcg cgcagcattc gctccggcag
     6841 tgggcgtttc actttctgct cgacaatatc acgcccgata tccagcaaat tgtgatagcg
     6901 cacaccagaa ggacaggtgg tttcacaatt gcggcaagtg aggcagcgat cgagatgctc
     6961 ctgtgtttta agcgtgactt cgttgccttc cagcacctgt ttaatcagat agatgcgccc
     7021 gcgcggcccg tccagttcat cgcccagaag ctgataggtt gggcaggttg cggtacaaaa
     7081 tccgcagtgg acacaggcgc gcaggatgct gtcggcttcc agcgcgcgcg cgttctgccg
     7141 catctcttcg gttaatcggg tttgcatagc ctgctcctca aagttccgcg tacatgcgac
     7201 cggggttaaa cacgccgcaa gggtcgagct gctgtttaag ctgctggtga tagcagaata
     7261 aaggagccga tagcggggca aagccaccat ctccggcact aaagcgggtc gcatgaccgc
     7321 cagcgttgcg ggcgatgcga tggatttgat tgtcatcggc tgtcgatttc agccagcgta
     7381 acgccccgcc ccagtcgatc agttgctcgc cgggtaaatc catcatcggc gcatcgctgg
     7441 gtaatgaaat gcgccataag gtacctggta acgagaagaa cggcagttgt tgttcacgca
     7501 attgctgcca gaactgaccc gcaacctctt cgccacccag cagttcacgc gctgctttta
     7561 ccgatccttc gccgccctca aggcggatcc acaacgcatt gtcgaagtaa cataagccac
     7621 taatgggtaa tggctggagt tgccactcgg cgatttcact catggcttct tgcaggctga
     7681 tttcccgacg caggctcaga gaggcacgcg gtcgcggtaa cactttcatt gagatttcag
     7741 tgagcacgcc aagacaaccg tagcttccgg ccattaaccg tgagagatcg tatccggcaa
     7801 cgtttttcat cacttcgcca ccaaaacgca gatgttttcc agtgccggta atgatgcgcg
     7861 tgccgaggac aaaatcgcgg accgaaccgc tccacgggcg acgcggcccc gccagcccgc
     7921 aggcgaccat cccgccccag gtggcttctt caccataatg cggcggctca cagggaagca
     7981 tttgcccggc gctctccagc gccgcttcaa tcgccaccag cggcgttccg gcacgagcgg
     8041 ttatcaccag ctcggtcggg tcgtaattaa caatgccgcg atgacaacgc acatccagcg
     8101 tttgcccggt gactgggcga cctaaaaagg ctttgctatt gctgccctga atcaccagcg
     8161 gcgttttatc gctaattgcc tgattcacct gctccagcag cgcctgactg taatcacact
     8221 cgcgtagcat caaaagcgct ccagttcagg aaaaggtaaa tgaccgtgat gcacatgcat
     8281 ggcaccaaat tcagcacagc ggtgtagcgt gggaatgttt ttcccagggt tcagcaaacc
     8341 atcggggtca aacgccgcct tgaccgcatg gaaggtcgtg atttcatcgc tgttgaactg
     8401 ggcgcacatt tgattgattt tttctcgccc gatgccatgt tccccactga tgctgccgcc
     8461 aacttcaacg cagagttcga ggatcttccc gcccagctct tccgcgcggg caaattcacc
     8521 aagttcgttg gcatcgaaaa ggattaacgg gtgcatgttg ccatctccgg catgaaagac
     8581 gttggcaaca cgtaaatcat attgctgcga taaacgggca atgccttcca gtacgccagg
     8641 cagggcgcga cgcgggatgg tgccatccat gcagtagtaa tccggggaga tacgtcctac
     8701 cgccgggaac gcatttttgc gaccggccca gaaacgtacg cgctctgctt cgtcctgtgc
     8761 caaacggacg tcagtcgcgc ccgctttcaa caagatgtag ttaacccgct cgcagtcttc
     8821 ctgtacgtca gactccacgc cgtccagttc gcataacaaa atcgcttcgg cgtcgacggg
     8881 ataaccggca tgaataaaat cttccgccgc gcggatcgac aggttatcca tcatctccag
     8941 cccgccgggg ataatgccat tggcgatgat gtcaccaacc gcaagtccgg ctttttctac
     9001 cgagtcaaag ctggctaaca gaacccgcgc cacgggcggc ttcggcagca gttttaccgt
     9061 cacttcggtg gtcacgccga gcataccttc cgatccggtg aacagcgcca gcaggtcaaa
     9121 accaggtgaa tccagcgcgt ccgatcccag cgtcagcgcc tcgccgtcca gcgtttgcac
     9181 ttcaattttc agcaggttat gtacggtcag accatatttc aggcagtgga cgccaccggc
     9241 attttccgcc acattgccgc caattgaaca ggcgatttgt gaggaaggat ccggcgcgta
     9301 atagagatta tgcggcgcaa cggcctggga gatcgccagg ttacatacgc ccggctgcac
     9361 gcgcgcgcgg cgaccaacgg ggttaatgtc gaggctctct ttaaagcgcg ccatcaccaa
     9421 caacacacct ttttccagcg gcagcgcgcc accagaaagc ccggtgcctg caccacgggt
     9481 caccaccggt acacgcaggc gatggcagac agccagaatc gctgtcacct gttccatttg
     9541 cttaggcaga acaaccagta atggacgcgt gcgatacgcg ctcaacccgt cacactcgta
     9601 aggaatgatc tcctcatcgg tatgcaggat ctcaagtcca gggacatgct cacgcagtgc
     9661 catcagtacc gatgtgcggt cgacatcggg taaagcgcca tcaagacgct cttcgtacaa
     9721 gatgctcatg agtaggcttc gctttgttgt gtggcagctg atttttgcgc gctgcttctg
     9781 tgaacagtta ttaagcgggc ttttcgtttt cgtctatctc tttagctacc ggtcagacca
     9841 ttttttttcc agctctgtga ccttgtcttg gttaacttaa tgttaaattg atgtaacata
     9901 atcacttgcg tgatgtgcgt gttttgcgag ttaagaacag aaaaattggt cctacctgtg
     9961 cacgaggtcc gggaatgaaa gatgaacgtc gccctatttg cgaagtggtt gcagagagta
    10021 tcgaacggtt aattatcgac ggcgtactga aggtcggtca gccgcttccc tcggaacgtc
    10081 gactgtgtga aaagctcggc ttctcacgct ccgcactgcg tgaagggctg accgtgctgc
    10141 gcgggcgcgg gattattgaa acggcgcagg gtcgcgattc tcatgtcgca cggcttaatc
    10201 gggtgcagga caccagcccg ctgatccatc tgttcagtac gcagccgcga acgctgtacg
    10261 atctgctcga cgttcgcgca ttactggagg gcgaatcggc aaggctggcg gcaacgctgg
    10321 gaacgcaggc tgattttgtt gtgataaccc gctgttatga aaaaatgctc gccgccagtg
    10381 agaaccacaa agagatttcg ctgatcgaac atgcgcagtt ggatcacgct ttccatctcg
    10441 ccatttgtca ggcttctcac aatcaggtgc tggtgtttac gctgcaatca ttgaccgatc
    10501 tgatgtttaa ttcagtgttt gccagcgtaa ataatctcta ccatcgacca cagcaaaaaa
    10561 agcagatcga tcgccagcat gcgcggatct acaacgcggt gttgcagcgg ctgccgcacg
    10621 tcgctcagcg cgcagcgcgc gatcatgtac ggaccgtgaa aaagaatctc cacgatatcg
    10681 agctggaagg ccaccatttg attcgctcgg cggtgccgct ggagatgaac aaggtgggaa
    10741 tgtgagcaga gttttaccct atccccaagg cgaggggacc gcccctgcac ttaacaggat
    10801 ctggtgttaa atctgttcat ctataccctc tcccgaatga ccgcccctgc gctaaacagg
    10861 atctggtatt aaatttgctt ttcatacaat cggtaacgct tgtacggctc cgccccaatg
    10921 cgttccagca tgttattcat gcctgtattg gtttcgagga tccattacat ctccagcgca
    10981 tcgatcttcc ggcgggcaaa cggatcgcgt aaggcttcaa tcaataacag cgcaatcacc
    11041 gggccgatgc ggctgaactg atactcgtcg cgcacgccca tcagcggtac tcgcgcagtt
    11101 cgcacaccgc tgactttcaa acgccacagc aattttgccc agccgaaggg aaagagcgat
    11161 ccgttcagat cggcaatcgc ctcgttgatg ttaggcaagc cgacaataaa cgcgcagggt
    11221 gcagaatcaa tctcagcgat gtagatcata tcgtccggca ccagatattt aagttggtcg
    11281 cccatggtcg cgaattcatg ttcggtaaac ggcacaaatc cccagttgtg ctgccagcca
    11341 gagttgaaaa tctcacgcag gatctgcatc tcttcggcaa accgctgacg attgatgcag
    11401 cgaatggtca cctttttgcg cacctgatcc atcagttttt ttagcgccgg agagaaagtg
    11461 agatcggttc gctgcatcca ccacgccagt aaatcaatgc ctttgtgata acccagttgt
    11521 tcaatatgcg cggcatacca cggtttgccg tgtggcatca tcgcacaggg tggtgtgtca
    11581 aaaccttcaa tcagtaatcc gctttcctga ttgatattca ggctgaaagg cccgctgatc
    11641 ttacttgcac cttgtgactt caaccacgct tccgctgcgc caaacaacgc ggcaaaatcc
    11701 tgcggatcat caatggcgtc aatcatgccg aaatgaccgg tatcttcacc gtaacgctca
    11761 cggtgcaagg tatctatttg cgcggtaata cgcccaacta tctgccccgc tttttttgcc
    11821 acccacgcct gccagatgat atggtccgtc cccggatttt tcgcagacaa atgctcgttg
    11881 cgttcaatga ataaaggggg tatccagttt ggatcgtcgg gataaagtga tgacggaaaa
    11941 gcgataaatg ccttaaggtc atttttatta attacttttt caattttaat catcagcaga
    12001 tcgcattcca tatctctgtg gataacaaat aattaatggt gaaaaacagg ttgcaacagc
    12061 aacgagcggc atcttaataa tcaactcaat gaatacaaac gacgaataaa tagtattaat
    12121 taatgattat gataaggtca gaggctacac agaaacgcct caatgccttt tgtcgattta
    12181 aggcgaccgg acaaggcttc ttcttaatat attcataaat taaatatcat tcataataaa
    12241 aataaaaaac tcaaactcat tatttaaatc aatgttcatt ttcacaccga tgaaaatact
    12301 atctcattta tcttttatgc ctatataatc caccgcatta ttgtcatttt atttttgata
    12361 tccaattttt tcgtggtgta tatgtctaat aaaatcttta cgcattccct acctatgcgc
    12421 tatgccgatt ttccaacgct ggttgatgct ttggactacg ccgctctgag tagagccgga
    12481 atgaattttt atgacagacg ttgccaactt gaagatcaac tggaatatca gacattaaaa
    12541 acgcgtgccg aagctggtgc gaagcggttg ttatcgctga acctgaaaaa aggcgaacgc
    12601 gtggcgctga ttgccgaaac gagtagcggg tttgtagagg ctttttttgc ctgtcagtat
    12661 gctggcttag tcgccttccc gttggcgatt ccaatgggcg tcggtcagcg ggattcctgg
    12721 agcgccaaac tctagggttt actggcaagt tgccagcccg cagccattat cactggtgat
    12781 gagtagttgc cactggtcaa tgccgcgacg cataacaacc ccgaattaca tgttttaagc
    12841 cacgcttggt ttaaggcatt accggaagcc gatgttgcgc tccagcgtcc agttccgaac
    12901 gatatcgcct acctccagta cacctccggc agcacccgtt ttccccgtgg cgtcattatc
    12961 acccatcgcg aagtgatggc taatctacgt gctataagcc acgatggcat taaattacgc
    13021 cctagcgacc gctgcgtctc ctggcagcct ttctaccatg atatgggact ggtcggcttt
    13081 ctcctgcccc ccgtcgccac gcaactttca gtagattatt tgcgcactca ggattttgcc
    13141 atgcgtcctt tgcaatggct taaattgatc agtaaaaatc gcggcaccgt ttccgttgcg
    13201 ccgccgtttg gctatgaatt gtgccaacgc cgcgtgaatg aaaaagatct cgctgaactg
    13261 gatctttcct actggcgcgt cgctggtatt ggtgctgagc cgatctccgc agaacaactc
    13321 catcaattcg ctgaatgttt ccgtcaggtt aactttgaca ataaaacttt catgccgtgc
    13381 tacggactgg cagaaaatgc gctggctgtc agcttctctg atgaagcctc cggggttgtg
    13441 gttaacgaag tggatcgcga catcctcgaa tatcagggta aagccgtcgc gccgggtgca
    13501 gagacacgcg ccgtatcgac tttcgtcaac tgcggcaaag cgttgccgga acatggtatt
    13561 gaaatccgca atgaagcagg tatgccggtc gcggaacgtg tggtaggcca tatttgcatc
    13621 tccggtccca gtctgatgag cggttacttt ggcgaccagg tttcgcaaga cgagattgcc
    13681 gcgacgggct ggttagacac cggcgacctc ggttatctgc tggacggtta tctgtatgtc
    13741 accggacgca ttaaagatct gattattatt cgtggccgta atatctggcc gcaggatatt
    13801 gaatatatag cggaacagga accggaaatt cattctggcg atgcgattgc ttttgttacc
    13861 gcccaggaaa aaatcatttt gcagatccag tgtcggatca gcgacgaaga acgtcgcggg
    13921 cagcttatcc acgcgctggc ggcacggatc caaagcgaat ttggcgtgac cgcggctatc
    13981 gatctgttgc cgccccacag tattccccga acgtcctccg gcaagcctgc ccgtgcggaa
    14041 gcgaaaaaac gttatcagaa ggcttatgct gccagtctta atgtgcagga atccctggca
    14101 tgaatcaaac tgtcgcggtg acgggcgcta ccgggtttat cggtaaatat attatcgata
    14161 acctgctcgc ccgcggcttt cacgttcgcg cattgacgcg tactgcccgc gctcacgtca
    14221 atgataatct cacctgggtg cgcggttcgc tggaagatac tcattcactt agcgagctgg
    14281 ttgccggagc cagcgtagtg gtccattgcg ccgggcaagt gcgcgggcac aaagaagaga
    14341 ttttcacccg ctgtaacgtt gacggcagcc tgcgcctgat gcaagcagca aaagagagcg
    14401 gcttttgcca acgttttctg tttatctctt cgctggcggc gcgccatccc gagctctcct
    14461 ggtatgcaaa atccaaatac gtcgccgagc aacgactggc agcaatggcg gacgaaatta
    14521 cgctgggcgt ttttcgcccg acagccgtgt atggtcccgg cgataaagag ttaaaaccgc
    14581 tgtttgactg gatgctacgc ggcctgctgc cacgacttgg tgcgccagat acacaactct
    14641 cttttcttca cgtcaccgat tttgcgcaag cagtgggtca gtggttaagc gccgaaactg
    14701 tacagacgca aacctatgaa ttatgcgatg gcgtcgccgg tggctatgac tggcaacgcg
    14761 tacgacagct tgccgccaaa gcccgttgtg gttccgtgcg aatggttgac attcctctgc
    14821 cggtactcac aggccttgcg gatatcagta ccgcgttgag tcgcctggca ggtaaagaac
    14881 ctatgctgac ccgctcgaaa attcgtgaat taacccacgc cgactggtcg gcaagtaata
    14941 accgtatttc tgaagatatc aattggtttc ccgggattag cctggaacac gcattacgca
    15001 acgggctatt ttgattgtct tgttttaaag gtgtgttaac gtttctatgg taaatcgtga
    15061 aatagtaatg gattacatcc tttcctgttt acaggatttg gtcgaaaacg gtgtggaaat
    15121 taagcccgac agcgatctcg tcaacgatct tggcctggag tcgatcaaag tgatggatct
    15181 cttaatgatg ctggaagaca gattcgatat ttccattcct attaatattc tgctgaatgt
    15241 caaaccccct gcgcaattaa tggaaactct acttccatgg ctggagaata aataatgggg
    15301 ctatacgata aatatgcgcg ccttgctggc gagcgtctgc aatttagtga caacgggtta
    15361 acaccgtttg gcacctgtat tgatgaagtg tattccgcca ccgaaggccg gatcggcaat
    15421 aaaaaagtga ttctggccgg gaccaataat tatctcggtt taacctttaa tcatgatgcc
    15481 atcagcgaag gtcaggccgc gttagcggcc caaggcaccg gcaccaccgg ttctcgtatg
    15541 gcaaacggca gttatgcgcc gcacctggcg ttagagaaag agattgccga gtttttcaat
    15601 cgccctaccg ctatcgtttt ttcaaccggt tataccgcta atttaggcgt catcagcgca
    15661 cttgccgacc ataacgccgt ggtgttactg gatgccgaca gccacgccag tatttatgac
    15721 gcctgttctc tcggtggcgc ggaaattatt cgtttccgcc ataacgatgc taaagatctg
    15781 gaacggcgca tggttcgcct cggtgaacgg gcgaaagagg cgattatcat cgttgagggc
    15841 atctacagca tgctcggtga cgttgcgccg ctggcggaaa ttgttgatat caaacgtcgt
    15901 ctcggtggtt atttgattgt cgatgaagcg cattcgtttg tcgtgttagg cgcaacgggg
    15961 cgcggactgg ctgaagctgt cggcgttgaa gatgatgtgg atattatcgt tggtactttc
    16021 agtaaaagtc tggcctcgat tggcggtttt gctgtcggtt cggaagcgat ggaagtgctg
    16081 cgctacggca gccgcccgta tatttttacc gcctctcctt ctccgtcctg cattgcgacc
    16141 gtgcgctcgt cgttaagaac tattgccaca caaccagagc tgcggcaaaa gctgatggat
    16201 aacgccaacc atctgtatga cggtctgcaa aaactgggtt atgagttaag ttcacatatt
    16261 agcccggtag tgccagtaat aatcggttcg aaagaggaag gtttacgcat ctggcgcgag
    16321 cttatctcgc cgggcgtgta tgtgaatctt atcctgccgc ctgccgcgcc tgcaggcatc
    16381 acgctgctgc gctgtagcgt aaatgctgcc cacagtcgtg agcaaatcga tgccatcatt
    16441 caggcatttg ctactctgaa gcagtaatgt gagcccgccg tggtgtttgc gccacggcgg
    16501 gtttaatgtt gtctgtcata ccaatacacc aggctcaacg ccaccaacac aaacgctacc
    16561 ggtaaaccga gcgcggtaac aggtgcgctt aacgcaaata gcaatcccag attaaccatg
    16621 atttgatagc tgatccaggt gagtaagccc acgatgacgc ctacagcgag acggctaccc
    16681 atcccaggcg aacgcggggc actaaaggta aacggcacag ccagcaaaat catcgccagg
    16741 gtcaggatgg gttgccccag tttttcccac agagcaaggc ggtattcgct gctgggttgc
    16801 ccggtttcct gcaaataatg gatgtaatga ttaagctgac ggacagaaaa agtattcccc
    16861 ggcattgata actcttcaag attcatactg gtgaaggccg attgccaggc aagattatct
    16921 aatgtttcca gcgtttcttt gccattcagc cattttttat gattcacacc atgcaggacc
    16981 cacgttttgt cgtcttcaat ggtggcactg cgtgcataaa tgtaggattc aagcgagaga
    17041 tcgtcgcgat aatgaaatat ctccacgccc accggctggc cctgctcgtt cagcgatttc
    17101 actgtcacaa attcattacc acgcctggcc cacagcatat tgccggtaat gtcatcgtcc
    17161 tcccccaacg cggtggcggt cgatttgatt tgcaacgcct gttgctgcaa tggcgacgcc
    17221 acccactcat ctatcgcgcc taacgaaaca gtccacaata tcccggcaac cagcgccacc
    17281 agtgcaatac ggaagataga aaaccccgtg ctgcgaatgg cggtaagctc actgtttttc
    17341 gataactgcc ccagtccaac aataccaccc aataacgcga taaacggcga aagttcgacc
    17401 agtgtgcgcg gtaaggtcat tagcaccacc agcaccgcct gagtccagcg ataaccgccc
    17461 gggctgacac catccagttc gttaatcagg ttgaaggtgg taaaaagcgg cagcaatagc
    17521 cctgcagcgg cggcaaaacc gagaaagaga tgacggatta aatagcgact gaaaacattc
    17581 atcgggaaaa tttctgcagc aaagagaagt cacgtgcgac cagtatcagc aggcccatta
    17641 gcatgagtcc gggcactaac cacaaaccag gaatgagggg aatcgcgccg ttagccacca
    17701 gtgtacggca gacgtcgccg ccataaaaaa tggcaataaa cagcaacgtc agtggcaata
    17761 acgttgaaaa tcgcccttgc cacggtttta cccgacttaa tgaaatggcc agcagcgcca
    17821 tcaacaatgc ggtcaggcca cggctttgtc gccattgcag ttcggcatga tcggcaggaa
    17881 aggcggagcg cgccagctcc gtgaccgatt tcgctttgcg tttgacgttc gggctttgat
    17941 ccagcggatt cagatgtaat tgcagattac ggtagatctg ttcgttgtcg tcacgcccct
    18001 gatgatcgag aagataggca gtgccgttgt gcaacataac ggtcggtttc tcgggtgatg
    18061 ggtcaaccac atcaaccgaa cgggcgcgga aaatacgggt tcgattggca gtagaagtat
    18121 aaatcagcgc gtcagtcagg cgattattat cctgatcaac cgtctgcgaa aggatcattc
    18181 gtccgttatc gttagtgtta aatttctttg cccgcaactg gcgaacatcc agctccgact
    18241 gcgactgctg ctccagttga taaatctgcg cataagccca cggacgacca tacatcgaca
    18301 gaagggtgac aaaaatactt agcggtatcg ccagataaag aacggctttg tacaagcgtc
    18361 ccggactgcc ccccgccgca gagatagcgg taatttccga gtcggtatac atctgcccta
    18421 gcgtcacgcc aactgacaca tacagaccaa caggtaacaa catctccagt gcaatcagta
    18481 ctttgtagaa aacgatatcc agcacaacat caagagctaa tgtcccattt gccgcttcgg
    18541 tcagataacg ctgtgcggag tagctggcga aaataaagat cagaaaaccg acgataatca
    18601 gcaccagacg gcgcgtacca cgcatgatgt agtgttcaac cagcttcatc tcactaacgc
    18661 cctcaatcct ttgagttttt tattcattgc tcagtttctg cgccaacgca atgacatgat
    18721 ctacctcttc cggcgtcagg ctgccgtcct ttgcccactg aatttgcccg gctttattca
    18781 gcaccagaat ggttgaactc tgctccggca atcgccaggc aacgcgtccc tgtccattgc
    18841 cgtcaataac gaattgcgcc cacggatagc ggcgtttatt tttttcgatt ttgccaacaa
    18901 caaaatagcc agtaccaaag atggcatcat cggtgttcac aatggtggtg ggctgaaaac
    18961 gatcctgcgg aaagtttgcc gcttcaacag ctttaattaa cagtgagttc tttttcttcg
    19021 ccgacttacg cccggcgata tactgaataa tgcggacttt tcccgctaat tgtgtgctgt
    19081 tccacttttg gtagtgaatt tcgtcgtcgc tattcagaag cagctcgccg ccttcctgga
    19141 tataaacagg ggtaaccgtt ttgccagtga cgaaattgtg tgcctgcaca cccggggcta
    19201 agagcatgat aatcaacgca ataatcaaac gagatgacat cattgctccg gctatagcgg
    19261 aaaacgcgaa atcagcagct ctaagcgctg acaaatatcg ctatccagcg ccaacggtgc
    19321 ataatacgac tggcggtttt ccatatcatc cgccaccatg tcgtcgtgtt tatctcccat
    19381 caggccatta atggcggcga ggaaacggga aatctgtaac gcctctttat tttcaggatc
    19441 aagcgatctt aaggtgtcgt aataagaaac ctgctggctg atgatttttc gcacatcatg
    19501 aaattccctg ccggttagtc gcggtttggc aataatctct ttcagggtat gcatttgctt
    19561 ttgccgatag gcaataaaac tgcttacgct ttctaactgg ctatggcgta atgaatagtt
    19621 caccaacgac cagaccggag agcttaaata aatccgtaac aggttgccgt aatacgacac
    19681 aatatttttc ttaccgttac gaaagccgtc ctgaaaacgc cccagaaaaa cggtcgtttt
    19741 gccgaataaa aatccggcct gatgcttctt taaatacagc cgttgcgcaa aacgcaggtg
    19801 cttgtagcgc gctcgcatgt atttaaattg tttgcgctca tcagcagtca gctcatcgcc
    19861 tttggcttgt ttgcgaacta aacgcagtaa ttcctgacga tcaacgccat tgagccagaa
    19921 ttgcaggcat aacgcgtaat tatcatgaat gatttcggtg gtacaactta gtgcaatagt
    19981 cggcggcaaa ctaacctgtg catcaacgac atcatcttct tcaatcgcaa caaatagacg
    20041 ctcgctcgcc tcaagcggga aatattttct catcatcagg atttctctcc ggctgcgtta
    20101 taaagcaagg aatggcgctg aaaaaatgcc tttaaggttg gaattaacga gagcgccacg
    20161 tccagacatt gcccaccgaa gtaagcaaac gctgctccca gcacaccaaa cagatgacag
    20221 aacatgaaca gcaatacgat gtagccgatt gaggcgatgg tttgcgccac gagaaaagcg
    20281 cgctgttttc ccgccatcaa tagcaatgac tcctgcggga agcccagcat cgaaatcaca
    20341 attgcgccca gcatcacctg aatcaggtca tacgcttcga gatacttaac gccaaacacc
    20401 agcgaaatga gcggcttgcc gacgatcaac accgcgagcg ccaccagaat accgattccg
    20461 cccgccagta aaccggactt cacacctaac aaccacggtc tggtggtgcg cggatctaaa
    20521 cgcatcacct ctgggtaaaa acttttaccc agcaaacccg ctggcgttcc ggcggcgtcg
    20581 aagaatgtca tggcgatttt aaataaccca gcggcagcgg gtcctaacac gatccccacc
    20641 aacactgtgc tgcacgagtt acgcgccgac cagatggagt gggcaatgtt ggttgaccag
    20701 acaaaactcc acgcgccttt aatgtgtcgg gcagactcaa acagattcaa tttgaaggcg
    20761 ttatggatat ttctacggcg taattcgcgc gcggcaaacc accagtacat ggtgccgcca
    20821 accagattcg acacgtacca ggcaataaca aaacccgcaa aaccaaagtc aaaataccag
    20881 gctgcgacgc tccccgctgc gcgcagaaaa ggtttcgtcg cctgctgtac agcaatcaaa
    20941 tcaaagcgat ctaccgcacg cagaatgccg gtcggcgtgg aggaagccat tgaaggaatg
    21001 agcgtgcaat acagcgccgc cagccaaaag ctttggtcat ccagacctaa tgaatgggaa
    21061 aggaatggca gtaaggcaat gccgccgaca atcgccaccg cgccgctgac gatatccagc
    21121 gagaaggaaa atgagacgac attgcgaaat tgctgcggat tattgttggt caaggctggc
    21181 gttccgtact gaaccaccag ctgccatgtc tgaaacttaa taaaatcgct gatcgacttg
    21241 gcgtacgatt gcacaatcac cagtacacca aacatggcgg gcgtcatccc tttaccggca
    21301 cacgagagcg ccaacagccc cagcaaggcg ctctcgacat tactggagcc taaccaggcg
    21361 ctattgcgaa taatggtgcg aaacgcgcca tctgcaaacc aatgtttgat gttaaaacca
    21421 gccaattcag cctgacctta tcgttgatag taaaaaatat cccgccagcc ttaagttaaa
    21481 cttcggcggt cagaaacgat ggcaaccaga gaaaccgcct tctgtacctg ttccagcact
    21541 tcgctgtagg gcgctctgga atcaatctca agaatttttg tgccgttata gccaatcgtc
    21601 gacatgacgc cgattttgtc ctgcagctcg gcatactcat ggtcaggctt gcgggagatg
    21661 gcagtatcaa tatcaatccc caaacgaatg attaattcag gacggtattg cgccattttt
    21721 tggtataaac tccgttcgcg ctgcgccaga aacatgctga ttttcccggt cgcacgttcg
    21781 acgccgatcc ccggtccgtc ataataaaag cccgaaattt cagcctgcgg gaagcgatcg
    21841 ctgaccacca gaacgccact ttgcgccagt cgctgaacct tgcgtagatt cgccattctt
    21901 cgcagcgaga agcagtacat aatcaccgcc gcccatagcg ccggagattt ggttttcatg
    21961 ctttgggttt tcgaggattt ggccgccagt cgccgttcca gccagacgcc aaccaacggc
    22021 aatcgtttga ttttgtctcc gtcttcgccg gagagcagcc ccagatagcg gcgctcggtt
    22081 tgccagtgtt gttgcagcga tttcaccagg tcggtggtca gcgtggattt accggtgcca
    22141 tcacacccga ccaccgcaat cagccccgga atgtagttgg gctgcggcgc ggttgtacta
    22201 ttaacagttt gtgtttgtag tgcatccatt cgttaaatca tcccctatcc attttggtga
    22261 tcaggaaagc gcggcgtgaa ttgcgcgtag tgacgcttgc ataatttcat cggctggttg
    22321 ccgaccatcc agttcaagga tcttggcgcc attgaatgtc agttgcggcg taacggcgat
    22381 tttttcctgt aacgctgcca gttggtggtc aggtttacgc gcaaacgcgg tttgttcatc
    22441 aatgccaaga cgaatcaaca atacgggtaa ataagacgcc atccattggt acagcttcaa
    22501 ctcgcgctgc cttaacattt ttatccaacc gttaccgccc gtggtttttg ccaattgcgg
    22561 gccatcaaag cgaaaacccg gcacttcaac ctgcgggtag cggtcggtga tgagcagata
    22621 gccttgctgg cttttacaca acattttgcg aaacttgtac gcccgccagc aggaaagcag
    22681 atagataacc agcgcagtaa tattgccagg cggtgttgag ggcttttcgt gtacatgtgc
    22741 cgctttactt cgcagataac acccacaagg tgcgccaata acagggagct gtgaaatcca
    22801 ttcgccaatt cgcccggacg attgcccgag ataaatgtgt tctgttggca ttcttgctgc
    22861 cagttcattt accaggcttg ccgtgagggt cgatttacct gaaccatcac atcctacaat
    22921 ggcaataaca cgcactggcg tggaattaat tattgacata ttacgttgat tcacgaaaaa
    22981 cccggcagta aataatgtat tgaatattac gtggtccgtg ctcagaatat ccgttcaacc
    23041 ttattgtgac aatggttaat tgtgacattg acctgaatta cgctttcata aaaacatatt
    23101 aaccaaataa atatttttaa tggatattta aattaaagga tatattcatg cagtcaataa
    23161 cacctccatt aattgccgtt attggtagcg atggttcagg caagtcaacg gtgtgtgaac
    23221 atcttattac cgttgtcgaa aaatatggtg ctgccgaaag agttcattta ggaaaacagg
    23281 ccggaaatgt cggtcgtgca gtgacaaaat tacctttgat gggaaaatcc ttacataaaa
    23341 caattgaacg aaatcaggtg aaaacagcaa aaaaattgcc tggaccagtt ccggcgctgg
    23401 taattacagc gtttgtcgcc cgtcgcttac tgcgctttcg tcatatgctt gcctgtcgtc
    23461 gtcgcgggtt aattgttcta accgaccgtt atcctcagga ccaaattcct ggcgcttacg
    23521 atggtacggt gttcccacct aacgttgaag gtggtcgttt tgtctcatgg ctggcaagcc
    23581 aggaacgtaa agcgtttcac tggatggcga gccataagcc tgatctggtc atcaaactca
    23641 atgttgacct tgaagttgcc tgtgcacgta aacccgacca taaacgggaa tcgctggcga
    23701 ggaagattgc cataacgcca cagttaacct ttggtggtgc acaactggtt gatatcgatg
    23761 ccaatcagcc tctggaacag gtgttggttg atgcagaaaa agcgattacg gattttatga
    23821 ccgcgcgtgg ttatcactag tcaaaaatgg aaatgcccga tcgccaggac cgggcatttt
    23881 caggaaggtt aaatcaactg caatgctatc cagtacagcc caccagaaag aaaaattgcc
    23941 gccggtaaag taaacaccca cgccatcagg atgctggtta ccgttttacg ctgtaaccca
    24001 ccgccgtcca ccaccatcgt ccctgctacc gcagacgaga ggacgtgtgt tgtggagaca
    24061 ggcatcccaa tataactggc aaggccgata gacactgccg ccgtcatttg tgccgccatg
    24121 ccttgcgcat acgtcatgcc gcgcttacca atcttctcac cgatggtcat cgctacacga
    24181 cgccagccaa tcatggtgcc aatgccgagc gccagtgcta ccgccatgat gatccacacc
    24241 ggagcgtact caatggtgct taacatatcg ctgcgcagtt tcttcagcag gttctggtct
    24301 tctttactga cgcctggcag tttcgccagc ttcgcggagg tatcagagat gcacagcata
    24361 atgcggcgca gctggctgcg ctgactcacg cttaacggct cgtaactttc catattgcct
    24421 ggcagcatcg ttttaacgcg cgcaatcgca tcaaaggtat ttgccggatg acagtgaaac
    24481 tctgttactt gcgtgccatc agtcgatgct gcaggcaatg gaggttccat ggcaatcaac
    24541 ttctgcggca gttcaggatg ctgttgcagg tagtgttcga agttggtaac ggcatcgcgg
    24601 gtacgggtaa tttcatagcc ggacgcattc atattgacga cgaagccagc aggggcaatc
    24661 cccaccagta ccagcattac caggccgatc cctttctgcc cgtcgttcgc gccgtgcgaa
    24721 aacgccacgc ctgcagcgga aacaatcagc gcaatacgcg tccagaatgg cggtttacgt
    24781 ttgccctttt tctttttgcg atcttccgga atgcggtgaa tacggtcacg ctttttcgtc
    24841 ccactccaga aacggcgcag caggaaaatc agaccacccg caatgaccag gccgacgata
    24901 ggggaaacaa tcagcgagga gaaaattttg gttacttcac gcaggtttaa cgcatccatc
    24961 accgatgagc cggttaacag cgcgttggtt aaaccgatgc cgataatcgc accaatcaag
    25021 gtgtgcgaac tggaggccgg taaaccaaag aaccacgttc ccaggttcca gataatcgcc
    25081 gccagcagca tggaaaagac catcgccaga ccgtgagttg accccatatt cagcaacaaa
    25141 tcggttggta acatatggac aatggcatag gcaacgctaa gtccgcccaa taacacgcca
    25201 aaaaagttaa aaaatgccgc catcaccaca gcaagttgtg gctgcatggc acgagtataa
    25261 ataacggttg ccaccgcatt cgccgtgtca tgaaaaccat tgattgcttc gtagaacaac
    25321 acaaatgcca gagcaagtaa taacaaaagc cccgtatata tatcaaggcc aacaaataaa
    25381 tttagcatat attagattac gccattttga atttacgaac ggacgcatta tcagtgactt
    25441 taacggcatg ggcaaagtga aatatcattt tttaacgata aaaagagtgt tatttcttgt
    25501 gtattagaca ataagcattc attcaaaacc tttagaaata atcaaaacac aggtattcca
    25561 ctggttaata tattcacata tgaaatgaat aaatatttga atatataaat attgaatatt
    25621 ttgattaatc cccgtactaa ttattcttca taatcagtac ggggttgcta caacatgtat
    25681 tactttttca ccacaattaa cggttcaata tcactctctt ttttgatgac cagtgattca
    25741 tcaccgcgca aacacgtccc accgtagttg ccgccaacgg tgaaggtaca tacctgaatg
    25801 tatttaccgt ccactttcgg caaacaccac agttgctgat agatgttttt ctgctcggca
    25861 aatttaccgc tggttttgtc cagcacctct tcatgatggc cgaccaggtc gatattgcta
    25921 ccacagcgac cggcgatcgg tttcactgcg taacctgttt tcaccaattc atcattaaca
    25981 gtgaaatcgg tatccagcag gtaacgatgg tgcgggaaca gcgaccagag gatcggcaga
    26041 atcgctttgt tgccggggat caccgtccac agcggctcaa agaccagcac ttccgggcgc
    26101 agcaatacgt cgataagacg cacttcgttt tgcggatgac cggtacggat tggcaccgca
    26161 gcaaactcac ggtcgctaac ttcacgaatc tgatcaaacg cggtttccca cgcccaggtt
    26221 ttccacacgc agttaaccag tcgcccttcc ccatcaatca gttgcccggc agcatcccag
    26281 cccagttcat ccagcccgcg caagatacgc gtttcaaagc ccgcctggtg cagcgcctgc
    26341 tccataaact gcgcgtgata gttttcctcg atatctttgt cctgcatgat atggacaaac
    26401 ggacgtgcac gactgtgttt ccaggcacca gccagttcgt taatcagccc ttccgccgga
    26461 ttgaagccgt tgcctttata gccctgctcc gcccaacgtt cgaggatcaa gcccgcttcg
    26521 gtatgacagg aggcggagtc ggcgttgtat tcgtaaacct tcaggccacg ctcatccatg
    26581 cagaaatcca tacgaccagt gatcatatgg tgacggcgac gctgccagga gagacgcaaa
    26641 cgtggccaga ggattttcgg aatgtcgaac agcgccagca ggttgtcatc tttcagcacc
    26701 ttgtcggttg cgtgaagata catcagatgc agctcgttgg tggctttaat tagctcctgc
    26761 tcggcactct cggtaatggt gtagtaatga tacggatcct gattgatcac ctgaccgttg
    26821 gcctgcacat aggcgttttg tagcggatct ttttcatcca gccatttacc gtcaaactgg
    26881 cctttgtttt ccagacgcgc tccactgatt ttcagcagct cgcctgcaat ttccggctgc
    26941 ggtaagctgt attcagtatc ttccgtctgg atcatccagc ccagaatggt ggtgtcatca
    27001 aaagtgtctt taagggtata gcagccgttt tcgaccacca tctccagctc gcgcgtccac
    27061 tgttgccctt gcggcaacgg ggaatgaatc acgttctgtt ccgcaatacg gactttgttg
    27121 ccatgcaatt gggtaatgat ggcgacatgg ccagtgtctt taaattcacc gcctttatcc
    27181 cagataagaa gcgcacccgc gaccggcgca cgcggcgagc cgttaggaaa tgcctgcaat
    27241 ggcaggatgt tgtcattaac cacttctcgc aggaagcgca gcgagaaaat ctcccacgcc
    27301 atacccacgt cagtaaagac cacaccgtaa ttcagaaaga gaaaacggcg agcaaattca
    27361 acgcattgcc acttgtggcc catatattcg tcgtcgatat agctacggaa tacggcgtca
    27421 tcttcgtatt cctgcggatc gagagaactg taatctgaag agtagattgc taccccgcct
    27481 ggggcgtagc ccaataatgt cccgaacggg gcatcctggc tggtcgttcc tttgctcatc
    27541 actttacctt taacaataca acctaagcag ttggcgtaat tgttgtgctc tgattacctg
    27601 ctcagcgaca ttaaccggac agaggtcaga ctaatcatac acctcatcgc agcggctggc
    27661 gcaaagggtt aaaaaattca cattctgtac agcaagtgac ctgctacact gcttcaacac
    27721 taccactcag aaggcaactc actatgacag acaatactta tcagcccgcg aaagtctgga
    27781 cgtgggataa atccgctggc ggcgcgttcg ccaatatcaa tcgcccgttt tctggtccga
    27841 cgcatgaaaa aacgctgccc gttggcaaac atccattgca actttattcg ctgggaacgc
    27901 cgaacggtca gaaagtaacg attatgcttg aggagctact ggcgctgggc gttactggtg
    27961 cagagtacga cgcctggctg attcgtattg gcgatggcga tcaattctcc agcggctttg
    28021 tcgaagtgaa cccaaactcg aagatcccgg cgctgcgcga tcatacgcat aatccgccga
    28081 tccgcgtgtt tgaatctggt tcgatcctgc tttatctggc ggagaaattt ggctacttcc
    28141 tgccgcagga tctggcaaaa cggactgaaa cgttgaactg gctgttctgg ttacagggtg
    28201 ccgcgccgtt cctcggcggt ggttttggtc acttttacca ttacgcgccg gtgaaaattg
    28261 agtacgccat caaccgcttt accatggaag ccaagcgcct gctcgacgtg ctggataagc
    28321 aactggcgca gcataagttt gttgcgggcg atgagtacac cattgcggat atggcgattt
    28381 ggccgtggtt tggcaacgtg gtgctcggcg gcgtgtatga tgccgcagag tttctggatg
    28441 cgggaagtta taaacatgtg caacgctggg cgaaagaagt gggcgaacgt ccggcagtga
    28501 aacgtgggcg tattgttaac cgcaccaacg ggccgctgaa cgagcagttg catgagcgcc
    28561 atgacgccag tgattttgag acaaatacag aagataagcg tcaggggtaa gggttggtgt
    28621 tcgtcgcagc aagccatcca ggccggataa ggcgttcacg ccgcatccgg caatcgtgca
    28681 caatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaagctaat catcacgcgc
    28741 tggtaatgtc gtaatccatt tggcgcagtg cgtctaatgt ggctttggct tcatcttcgt
    28801 cgatgatgct catggcaaat ccgacgtgta ccagcaccca ctggcccagc agatcggcag
    28861 ggttaccttc gcaaatcagg gcgatattca cgtcgcgctt gataccacat acttcaacct
    28921 gcgcaagctg gtgaatatct tcaccgacag ccagcacctg gcctggaacg ccaatacaca
    28981 ttgttaactc cggttatttc gattgccgga caaacacgaa aaagaacctt tagcgggtgt
    29041 tccctctcac cctaaccctc tccccggagg ggcgaggggc ccgtccgtgc gctttttagg
    29101 cgttgtcctc agtcttattc aacttcaata cttttgacga tcagcgaatc gccggtatcg
    29161 acacgcaacc gctcgccgtg acagagcgga cactgcgcat cgtgctgatg aatctccacc
    29221 acctggctgc aatcccagca ccaggcctgg gcgggtttat agacgatatg taaatcgcac
    29281 ccttgcgcca ccgttccgtg gcagacaatt tcaaaactaa aacggacggc gctctcctca
    29341 acgcaggaga gcgcgccaat ttccagccac acggcggtga cgcgcttaac atcgtgctgc
    29401 tccgcctgcc gttggataat ttcaacggcg ctctggcaaa gcgacaactc atgcattttc
    29461 gcccctcctg cggccaaaca gcagggcgcg acgccctgcg tgtggtacat ccggattggt
    29521 gactggcaac gaaaggatca ttcgtgcgca gtcatccgtc aggcgttggc cttcttcaat
    29581 cgacatgctg tgcgaaagcg gcgacatcag cgaacaggag aggtattgcg aaacaccgtc
    29641 cagttcgcca acggtaaagg tcatggtgcc atacggcagt tgcagaccaa ttttttcact
    29701 cactttgcgc agcggccaga gttgatccgg gccggggaag ataactgcac tcagcatcca
    29761 cggggtgatc acacaccccg tccactgacc ttcgaacagc gtaaaatcag aaacatacac
    29821 cggcattgaa ggatgcagaa aagagagatc gtgcatcgaa cgccgggcaa tttcttcaaa
    29881 cgctgcctgt acttgcgcct tcggggaggt ctggaaacct gctatctctt cagtcatgaa
    29941 tcgcctcccg tgggatggct tccacgcctg attcgcgcag cgcagccaga accagctcaa
    30001 gcgcaggttc aatcattgct tcaaccgtcg gcgttaagcc gatgtgtggc tccagcgatt
    30061 ccgggatcac gccgaccagg gtcagctttt tcggaaactc accggtgaag cgcagggccg
    30121 acaatacgtc ggccaggcca agctgatgcg gagagatttt gttggtaaac aacgccggaa
    30181 cttcttcatc ccgcaggatc atcatcgttc ccggcgtgtt ctttttcgac acaatggcat
    30241 ccgcaataat cagatgatcg cgatttgcca tgtcgctaag cagctccatt cccgccgtgc
    30301 cgccatcgag gatctcaaca taatccggca gaatgtatcg ttgctctaac gcttcgacaa
    30361 tccgcacgcc gatggcttca tcggtcagca aaatattgcc gacccctaag actaaaatac
    30421 gcattacaga accttcactg aaaccacttc gttgccgtcg gcatccacta cgtgtaccgc
    30481 acaggccatg cacgggtcga aggagtgaat ggtacgcacc acttccagcg gtttattcgg
    30541 atcggcaacc ggtgtaccca ccagcgactg ctcgtaagga ccgacgtcat cattgaagtt
    30601 acgcggacca gagttccagg ttgatggaac aaccgcctgg tagttgctga tgataccgtc
    30661 tttaataacc atccagtgag agagcatacc gcgcggcgct tcgaggaagc caacaccttt
    30721 gaactcaccc gttgccggaa tgttcggctt cacaaaggtg gtgtgatcgc ctttgccgat
    30781 attggtgatc agtgcactgt attggttttg caggatatcc tgcaattcac agcagtgaac
    30841 ggtacgacca ataatacggc ccagcgtgga gtgcagctgt gccacttcca gcgtgttgcc
    30901 agtcagtttc tgataaatcg caacgatttc attcagtttg ttctgggtag attcgcgacc
    30961 tgccgccagt ttcaccagca tattagccag cggacccact tctaccgttt tgccgtagaa
    31021 agtcggtgat ttcacccagg aatatttccc gtcgtcagac caaccatcat aagccggaat
    31081 ggtggtacct tcccacggcg cctgtggcgc ttcgtcttta taccaggagt gcttcgcgct
    31141 ttcctgaatg cctttgatca ggtattcatc ggaatgagaa gtgatcggac gatacgagga
    31201 cagatccgca ttctcaatgt agccgcccgg gaacaggaag ctgccgtttt tgctgtcggt
    31261 cgggaattcc ggcacgctca ggtagttcac cgcaccttta ccgcgcgtca gccattccgg
    31321 gtagaacgcg gcgataaccg cggtatcaac cttataaacc tgctcaacaa agtcgctcag
    31381 tttgtcgatg aaagacttga tgtacatcag gcgctcaagg ttcaacacgc ccaacccgtc
    31441 gaggttgatt gggttcgcga caccactcac tgccaggttc tgaatgtgcg gcgttttacc
    31501 gcccagcagc gccacgacgc ggttagcgtc acgctggcac tccagcgcct gcaggtagtg
    31561 cgctaccgca atcaggttca cttccggcgg cagtttcatc gccgggtgac cccagtagcc
    31621 attggcgaaa atacccaact gaccgctggc aaccagatct ttgatcttgt tctgaacttt
    31681 agtgaactct tccggactgt tcagatgcca ggtcgaaacg cctttcagca tttcggaggc
    31741 tttggttggg tcagcttgca gtgcagaagt gatgtccacc cagtccagcg ccgaaagctg
    31801 atagaaatga acgatatggt catgcgtggt gtgcgcagcc agaatgatgt tacggatgta
    31861 ttgcgcgtta accggaacgt cgatattcag cgcactttct gccgcacgaa cggaagacag
    31921 cgcgtgagtg gtagtacata cgccacagat acgttgcaca atcatccatg catcgcgcgg
    31981 atcgcggttt ttcacgatct cttccatgcc gcgccacatg gtaccggaag cccatgcttt
    32041 cgaaacgacg ccattttcga tttcgcaatc gatgcgtaaa tgcccctcaa tacgggttac
    32101 cggatcaata gtaattctct ggctcatgct ttgctcgcct catgacgatt atgatcgttt
    32161 tgttttaaag gaggaagtat cggcagtaga cgaatgagta cgatgtaagc gcaaatctca
    32221 atagccacaa aaccaataga aatcaacagt tcttcccagg tcgggaagta ggcgtaaccg
    32281 ccgcccgggt tgaatgccac caacgaatag gtcagacgcc aggttgcaca acctaacagt
    32341 gcgctcagtg ctgacaggaa cagcatgcgg gaatcattac gcagcttcgc cacacgcaga
    32401 acgaccagcg ggaagagcat cagcaggact tcaatccaga acatcacgga gtagaagtca
    32461 ccggcaaacg ctaacgacag cttgtcgcga tagataagct cgccaaagcg cagcccgatg
    32521 aaaatcgcca gcaacacact gatggtgttg gtcagcttga cgaacaggct cttttcatcc
    32581 ggaccgttgc cacgcagacc cgcctgcacc agtgaacctt caaagatgac aatcgagaag
    32641 cccatgatga acgccgtcag cagcgagaac agcggcaaca tttcatagct ctgccagagc
    32701 ggatgcacct tgtagcccgc cgagatcatc agcgacccca ttgaagactg gtgcatggtc
    32761 ggcagcagcg caccgagcgc gatgatgaag aacatcacct tgttcagacg ttgtagcgac
    32821 accttccagc ccagacgttc aaacagtgcc ggagcaaact ccagagccat cacgccgatg
    32881 tagatggtca tacagaccgc cgtctcgaac agtaccgagt tcacgttgaa gtgacccgga
    32941 atgtagaagt acggcaggtt ccagtagcga cccacgtcga tagtgatcga caagccaccc
    33001 agtgagtaac caaacagact cgccaacagc gccggacgca ccagcggatg gtattgccca
    33061 cggttaaaga cgtataccgc ccacgccagc gcccagccgc cacaggcaaa gccggtgcca
    33121 atcagcaggt caaacgcgat ccacacgccc cacgggaagc cgccgttcag gtcagagaca
    33181 gagcccagac cgaacaccag acgcttcaca atcaggagca tacagatgac gattaacggt
    33241 ccaaaaatca tgaccggttt actgatgatt ttgccaccca gcggttgtgg atcatgactc
    33301 atgatcgtct cctccgtcgt gatggtcgtt tttggtgttg cgacgaacca gcacggttaa
    33361 gcccgccagc acagccagtg gtagcatcat gcctttatac agggtgtgtt gaatattttc
    33421 ggaacgcgca ccggtagaaa gatcgtccag tttcggcagg tcgagatttt cataaggcac
    33481 acccgtcagt accagaacct gagtaccgcc gccctctttc tcgccgtaca gatgcggata
    33541 atatttcggc accgtatgca ggtaagtgtc gccagatttc agcgtctgac gcggatagtg
    33601 gtattcgctg ccaggcttca gcgccagacg ttttttcgcc tccgccatca gctcttcacg
    33661 cgtaccgaaa atcaccgcgc ccgccgggca cacttctacg cagccaggca gaccgccttt
    33721 atcgagacgt tccacacctt tctggttgca cagctcgcac ttatgcagcg caccaaacgg
    33781 gttgttgtag tcgtacttcg gcacgttgta cggacaggcg accatgcagt aacggcaacc
    33841 ggtgcacacg tctttgtcgt aatggacaat gccggttttc ggatcttttt tcagtgcaga
    33901 gaccgggcac acagagacac agttcggatc gacgcagtgc atacactgtt tcttaatgta
    33961 cgcgtagccg ttctcctcct ggtctttgtt gacccctgtg ccgctggtcc acacctgaat
    34021 gatgttattg gtatacggcg acagtttgtc gttgttcgac caggtctgtt ccccttgcgg
    34081 gttacgttca gggaagttga tatcctgaca cttggtgacg caagcctggc agcctacgca
    34141 caaggtcgag tcgtacaaca tccccagcga tcccggaatt ggcgggcggt tttcagcagc
    34201 cgcatgactg acagacggca gcgcgcccgt cagcaatgcc ccgcaggagg ctgctttaat
    34261 aaaattacgt ctgttcacgg ttattctccc cgtgagtcag cgttatcttt cttttgctga
    34321 cgacccagtt cacgcaccgc catcacgctg acaccggcaa ccagcccaac cacaccgccg
    34381 agcaaaccaa tagcgcctgc agagacgttg ccgccctctt tagcgttaac atccggtttc
    34441 tgtgaacgcg gagtctgatt ttcgacgttg gcaagctgat ggatgccttt atggaagccg
    34501 ataccttttt cgttacagcc atagcacggg tgaccaatcg ccaccggcca cacaccgcca
    34561 acatcgcaga attgcagcgt tgagcagttg ccgtaagttt ctggcccttt acagccgagg
    34621 tggtacaggc accagccttc gcggtggcct tcatcaccga actctttggc aaaacgacca
    34681 gcatcgaagt gcgggcgacg ttcgcagtgt tcgtgaatca gacggccata ggcgaaggtc
    34741 ggacggtttt tgtcatccag tttcggcggt ttgccgtaag tgatgatgtg cgcaacggtc
    34801 gcgaggaagt tgtgcgggtt cggcgggcag cccggaatat tgataacggt tttgcctggc
    34861 agaacttctt gcaagctgac tgcgccagtt gggttaactc cagctgcggc aacaccgccc
    34921 cacgcggagc aggaaccaat agcgataatg gctgctgcgc cttccgccgc tttgcggatg
    34981 tgatccacaa tcggctcacc ggcaaccatg caataaatac cgttatcttt taatgggatg
    35041 gaaccatcca ccactaacac atactgccct ttgtacttct cgagagcgtt atgtttgttc
    35101 tcttcgacct gatgaccgaa ggcggcggaa agcacttcgt gatattccag agagatagtc
    35161 tccagcacga ggttttctac cgttggatgc gttgcacgaa gcagagattc cgtacaaccg
    35221 gtgcactcct gcgcgccaat ccagataact ggcggacgct gcgggttagt aaccgattcg
    35281 gccatctctg cagcggcttt gctacttaac cccatggtgg cggctaatgc tgcacaaagc
    35341 ttcatgaaat cacgacggtt aatgccgtga gaatggatga gggtgttatc tccagtcatt
    35401 tatagttatt ccgttgcgag gacctggcat atattttgcc tcaatcgcaa aatcaataat
    35461 gcgatcgatg cgccatttac cacacattta ttatggttat cgatatcatg atactgcggc
    35521 ggggcgctaa aacgaaggga agaagcatta gtgtaattaa ttaaagcaag ataatacgta
    35581 tgtttgatca attttcgagg cgataaccgg ccataaaaaa gcgcaacgta gaacaggaat
    35641 tattctcacg aaagaaatcc attaccatct ctttatccag tccatagcgg cgcgtcagaa
    35701 tacccgcttc ccaggcgcta agctggcgat ctccggtctt ttcgtacatc cggtgtgagt
    35761 agcctaatac aaaaccgcgt ttataatcgg cacagaattt tgtcacatta accgcactgt
    35821 cggcatgcgt tgcttttaat cccgccatta agcctttacc aaaatggtta ttcatcgttc
    35881 accccattcg ttatatatcg ccatatatag cgatatttta ggcaaagaac gacttttgcg
    35941 cacgctattt tttcatactt gtttaagacg taatcccgta agagataaac ctatcaggag
    36001 agatatttat cgaaccattt cagtgtcctt tgccaggcaa gatcggcggc agatttgtca
    36061 taacggggcg tggaatcatt atggaatccg tgattaaccc ccggatagat ataagcctca
    36121 taaaccttat tattgtcttt caacgccgcc tcgtaaggag gccagccctc gttgattcgg
    36181 gtgtccagtt ccgcgaagtg gagtagtaaa ggcgcttcaa tcttcgccac atcggcagtg
    36241 ggtgcctgac gaccataaaa cggcaccgcg caggccagtt ccggatacgc gacagccgcc
    36301 gcgttcgata cgccaccgcc atagcaaaat ccggtaatac ccactttacc tgtcgcttgc
    36361 ggatagcgtt gcataaactc aattgcggca aagaaatcat tcatcagttt ggttggatca
    36421 acctgctgtt gcagctcacg acctttatca tcatttcccg gataacctcc aacggaactt
    36481 aatccgtcag gtgccagggc gatatacccc gccttcgcca ctcgccgtgc cacatcttcg
    36541 atatacggat tcagtccacg attctcatgc accaccccca cggctggcgt tttgccgctc
    36601 atttttgcgg gtttcaccag ataaccccgt acctcgccgt gaccatttgg cgaaggatac
    36661 gtgatgtact cagcaacaat ctccgggtcg gtaaactcta cctgagtcgc cagcgcataa
    36721 tttggcttga gcaaatcaaa caacgctaat gccgtcatcc cgcccaccgc atacttcgcc
    36781 gcaaggttga ggaactcacg tttcgaaatt ttcccgtgaa cgtaatagtc gtagtaatcc
    36841 aacaactctt gtgggaaatc tttggcggtt aaacgcggca tcgttgcatt cctcaactgg
    36901 tgttttttta agcaaagcat aagcgcgtgt ttttgcccag tttttcgtca ctctgtgagc
    36961 cagactacgg gatacgcgct ggcgaatcgc taaactagaa acattgtttc gaaattgaac
    37021 ggtggaaagg agaggtcatg gtctggttag cgaatcccga acgttacggg cagatgcagt
    37081 accgctattg cggaaaaagt ggtttacgcc tgcccgcgtt atcgctcggt ttatggcaca
    37141 atttcggtca cgttaacgcg ctggaatcac agcgtgcaat cctgcgtaaa gcgtttgatt
    37201 taggcattac gcactttgat ttagccaaca attacgggcc gcctccagga agcgcagaag
    37261 agaactttgg tcgcctgctg cgcgaggatt ttgctgctta tcgcgatgaa ctgattatct
    37321 ctaccaaggc tggctacgat atgtggcccg gcccgtacgg ctctggcggt tcacgtaaat
    37381 acctgctcgc cagcctcgac caaagcctga agcgtatggg gcttgagtat gtcgatatct
    37441 tttactctca tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct gcgctggctc
    37501 atgcggtaca aagcggtaag gcgctgtatg tcgggatctc ctcttactcg ccagagcgga
    37561 cgcaaaaaat ggtcgagttg ctgcacgagt ggaaaattcc gcttttaatt catcaacctt
    37621 cgtacaattt actgaaccgc tgggtggata aaagcggcct gctggatacc ctgcaaaata
    37681 acggcgtggg ctgtattgcc tttactcctc tggctcaggg attgctgacc ggaaaatatc
    37741 tcaacggcat tccgcaagat tcacggatgc atcgtgaagg gaataaaatt cgtggtctga
    37801 cgccgaaaat gcttaccgaa gccaacctca acagcctgcg cttattgaat gaaatggcac
    37861 agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa gatgatcgcg
    37921 tgacgtcggt attgattggt gccagccgcg cggagcaact agaggagaac gtgcaggcgc
    37981 tgaataatct gacatttagc accgaggagc tggcgcagat tgatcagcat atcgccgatg
    38041 gcgaactgaa tctgtggcag gcgtcgtccg ataaatgacc tgttaataac gggcgcgcga
    38101 accgcgcccg aatcagatca gtgattatga cgagtcagtc ggtcaagata gcccatcaca
    38161 aatgcagaaa gcacaaacgt cagatggata atgacgtacc acatcagttt gttatccggg
    38221 acatttttcg catccataaa gacgcgcagt aagtgaatgg aagaaattgc cacaatcgac
    38281 gctgctactt tgtttttcag cgacgttgcg tccattttcc ccagccagtt cagcttctct
    38341 ttgttctcgg agatatccag ctgtgagacg aaattctcat aaccggaaaa catcaccatc
    38401 accagcaaac cgccaaccag cgtcatatcc accagcgaca gcaacacgag gatcaaatct
    38461 gattccgcca tcgagaagat attcggcagt acgtgaataa tctcctggaa gaacttcagc
    38521 gccagggcaa ctaacgccag cgaaaggcca aagtacacgg gggcaagcag ccagcgagaa
    38581 gcatacattg cattttcaaa aaaacgttcc atacattcct gtcgtgtagt taaaacgggc
    38641 aacagtatat cgcaattgag taaacagcac gcaacagcaa atcaacatat acgttaaacc
    38701 tttgtcgcat aattctgagg tttagggatc ttcctggttc ccttatcaac gcatcgagag
    38761 cgtctaccct taaaggacgt tgaacctgaa gggagaaaaa cgatgtctca tttaaaatga
    38821 cccgaccacg cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaggcat
    38881 ccaggcgaag atgacaccga taccggattg cggcgagaaa acctatgttg gtagcgctcg
    38941 cctgaaagat cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc
    39001 catcgcttac gcgcgagaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga
    39061 agacgctcag gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc
    39121 aggcgattta agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc acaaggcgtt
    39181 aggcgggctg gatattatgg cgctggtcgc cgggaaacag gttgccattc cggatattgc
    39241 agacctcacc agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg
    39301 gctaacccag gaagcgatcc ccctgctacc gaaaggtgca agtatcatca ccacttcgtc
    39361 aatccaggca taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat
    39421 tctgaactac agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat
    39481 tgtcgcgcca ggccctatct ggacggcact gcaaatttcc ggcggacaaa cgcaggataa
    39541 gatcccgcag tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg cggaactggc
    39601 ccctgtatat gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg
    39661 cgtgtgcggc ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca
    39721 tcattgtgaa ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat
    39781 gaagattcaa catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt
    39841 gagcacctaa tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg cacgaagatt
    39901 caacatgttg caatagattt tgtaggcctg atgagcgtag cgtatcaggc aattctactt
    39961 tacttcgctt tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc
    40021 agcgtatcca ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag
    40081 atggtggtgt ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg
    40141 gtgaccggat cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt
    40201 ttttccgggc gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc
    40261 gtcgctaacg gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac
    40321 ggcgtcacgt tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt
    40381 gccatcggac attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg
    40441 ctggcttcca gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc
    40501 agcatcgctt taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga
    40561 atcgctgcca cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg
    40621 actgccaggt tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag
    40681 accgtaccaa acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta
    40741 ccgcggccca tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt
    40801 tctttaatac cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca
    40861 ttgagcaaat gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg
    40921 tttaaggaac gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg
    40981 aagaactcta cgctcttact gaagaagatt gcccaggtga ctacggaggc caaaataagc
    41041 ccaatcatca cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg
    41101 tccgtctgca ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc
    41161 gcataataat atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg
    41221 ccgtaccttt gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt
    41281 tgcgaaaatt ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag
    41341 tttagacatc cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac
    41401 tcaactggtg aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa atagcgtgat
    41461 tcagcgcgct tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa
    41521 tcgcgccaat ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca
    41581 acaagcgatg tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc
    41641 agcggttgct aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa
    41701 caccgcctat gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac
    41761 gacatcgtgg tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac
    41821 taaaatcgtg tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc
    41881 gattgttgcc gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg
    41941 ggcagccggt gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaggccgc
    42001 caccaaatat ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg
    42061 ttgctgggag cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac
    42121 cgcctatata accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga
    42181 aagcagtctg aaagtggccg aatggctggc agaacatccg caagttgcgc gagttaacca
    42241 ccctgccctg cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag
    42301 cgggctattt tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct
    42361 ggataacttc agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct
    42421 ggcaaatcaa ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg
    42481 gaccttgatt cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga
    42541 cgccggtttt gcacggattg tataacattg ccacttttgg acaattttgc agacatttta
    42601 ttgtgaaaag tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta
    42661 caataggcag ataaaagctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag
    42721 atatcatcgc tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta
    42781 gcgttgttta ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct
    42841 catttttgcc aggcgacagc ttgttgatac tggcaggcgc attgattgcc caaggggtta
    42901 tggattttct gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa
    42961 gttatattca ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc
    43021 ttcctgctaa atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc
    43081 tgctggctgg acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt
    43141 ccggtctgcc aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca
    43201 gcgtggtaac cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag
    43261 atcaggtaat gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag
    43321 gcacgctgtt tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc
    43381 cggctgctgt accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct
    43441 tcccccggcg tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca
    43501 tagccgacgc gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc
    43561 gccttatgca gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta
    43621 aaattatggt ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc
    43681 aggttttcgg tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag actgaagtga
    43741 gtctggcgac tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac
    43801 agaatttcgc ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg
    43861 tcgagtaacc gctccgccgc gaataaaatc tcttctgata acgtggcgga gttaatcccg
    43921 ctggctgcca tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt
    43981 aactgcaaaa tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag
    44041 gtttcgcact caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga
    44101 aacacgcg
//
LOCUS       DADLTF010000013        44034 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.013, whole genome shotgun sequence.
ACCESSION   DADLTF010000013 DADLTF010000000
VERSION     DADLTF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.013"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(402..2678)
                     /gene="clpA"
                     /locus_tag="JN749_000728"
     CDS             complement(402..2678)
                     /gene="clpA"
                     /locus_tag="JN749_000728"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HAY7367238.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(2709..3029)
                     /gene="clpS"
                     /locus_tag="JN749_000729"
     CDS             complement(2709..3029)
                     /gene="clpS"
                     /locus_tag="JN749_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HAY7367239.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            3352..3576
                     /gene="cspD"
                     /locus_tag="JN749_000730"
     CDS             3352..3576
                     /gene="cspD"
                     /locus_tag="JN749_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HAY7367240.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            complement(3649..5595)
                     /gene="macB"
                     /locus_tag="JN749_000731"
     CDS             complement(3649..5595)
                     /gene="macB"
                     /locus_tag="JN749_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HAY7367241.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5592..6707)
                     /gene="macA"
                     /locus_tag="JN749_000732"
     CDS             complement(5592..6707)
                     /gene="macA"
                     /locus_tag="JN749_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HAY7367242.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            6858..7814
                     /locus_tag="JN749_000733"
     CDS             6858..7814
                     /locus_tag="JN749_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HAY7367243.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            complement(7811..9469)
                     /locus_tag="JN749_000734"
     CDS             complement(7811..9469)
                     /locus_tag="JN749_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAY7367244.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            9894..10589
                     /gene="aqpZ"
                     /locus_tag="JN749_000735"
     CDS             9894..10589
                     /gene="aqpZ"
                     /locus_tag="JN749_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HAY7367245.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     VSGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            11046..11945
                     /gene="lysO"
                     /locus_tag="JN749_000736"
     CDS             11046..11945
                     /gene="lysO"
                     /locus_tag="JN749_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HAY7367246.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            12089..13741
                     /gene="hcp"
                     /locus_tag="JN749_000737"
                     /gene_synonym="priS"
     CDS             12089..13741
                     /gene="hcp"
                     /locus_tag="JN749_000737"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HAY7367247.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            13753..14721
                     /gene="hcr"
                     /locus_tag="JN749_000738"
     CDS             13753..14721
                     /gene="hcr"
                     /locus_tag="JN749_000738"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HAY7367248.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            14854..16572
                     /gene="poxB"
                     /locus_tag="JN749_000739"
     CDS             14854..16572
                     /gene="poxB"
                     /locus_tag="JN749_000739"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HAY7367249.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            16609..17610
                     /gene="ltaE"
                     /locus_tag="JN749_000740"
     CDS             16609..17610
                     /gene="ltaE"
                     /locus_tag="JN749_000740"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HAY7367250.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            17621..19051
                     /locus_tag="JN749_000741"
     CDS             17621..19051
                     /locus_tag="JN749_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7367251.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANKHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            19150..20163
                     /locus_tag="JN749_000742"
     CDS             19150..20163
                     /locus_tag="JN749_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7367252.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVLTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(20160..20990)
                     /locus_tag="JN749_000743"
     CDS             complement(20160..20990)
                     /locus_tag="JN749_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAY7367253.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(20987..21310)
                     /locus_tag="JN749_000744"
     CDS             complement(20987..21310)
                     /locus_tag="JN749_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAY7367254.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            complement(21521..23291)
                     /locus_tag="JN749_000745"
                     /pseudo
     CDS             complement(21521..23291)
                     /locus_tag="JN749_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            23940..24455
                     /locus_tag="JN749_000746"
     CDS             23940..24455
                     /locus_tag="JN749_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7367255.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            24673..25401
                     /gene="artP"
                     /locus_tag="JN749_000747"
     CDS             24673..25401
                     /gene="artP"
                     /locus_tag="JN749_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HAY7367256.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            25419..26150
                     /gene="artJ"
                     /locus_tag="JN749_000748"
     CDS             25419..26150
                     /gene="artJ"
                     /locus_tag="JN749_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7367257.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            26157..26873
                     /gene="artQ"
                     /locus_tag="JN749_000749"
     CDS             26157..26873
                     /gene="artQ"
                     /locus_tag="JN749_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HAY7367258.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            26873..27541
                     /gene="artM"
                     /locus_tag="JN749_000750"
     CDS             26873..27541
                     /gene="artM"
                     /locus_tag="JN749_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HAY7367259.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            27833..28564
                     /gene="artJ"
                     /locus_tag="JN749_000751"
     CDS             27833..28564
                     /gene="artJ"
                     /locus_tag="JN749_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7367260.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(28739..29866)
                     /gene="rlmC"
                     /locus_tag="JN749_000752"
     CDS             complement(28739..29866)
                     /gene="rlmC"
                     /locus_tag="JN749_000752"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HAY7367261.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(29907..30395)
                     /locus_tag="JN749_000753"
     CDS             complement(29907..30395)
                     /locus_tag="JN749_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HAY7367262.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(30455..31300)
                     /gene="potI"
                     /locus_tag="JN749_000754"
     CDS             complement(30455..31300)
                     /gene="potI"
                     /locus_tag="JN749_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HAY7367263.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(31297..32250)
                     /gene="potH"
                     /locus_tag="JN749_000755"
     CDS             complement(31297..32250)
                     /gene="potH"
                     /locus_tag="JN749_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HAY7367264.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(32317..33393)
                     /gene="potG"
                     /locus_tag="JN749_000756"
     CDS             complement(32317..33393)
                     /gene="potG"
                     /locus_tag="JN749_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HAY7367265.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            complement(33488..34600)
                     /gene="potF"
                     /locus_tag="JN749_000757"
     CDS             complement(33488..34600)
                     /gene="potF"
                     /locus_tag="JN749_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HAY7367266.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(34952..35428)
                     /locus_tag="JN749_000758"
     CDS             complement(34952..35428)
                     /locus_tag="JN749_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HAY7367267.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(35516..36418)
                     /gene="rimK"
                     /locus_tag="JN749_000759"
     CDS             complement(35516..36418)
                     /gene="rimK"
                     /locus_tag="JN749_000759"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HAY7367268.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(36479..37202)
                     /gene="nfsA"
                     /locus_tag="JN749_000760"
                     /pseudo
     CDS             complement(36479..37202)
                     /gene="nfsA"
                     /locus_tag="JN749_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
     gene            complement(37186..37473)
                     /locus_tag="JN749_000761"
     CDS             complement(37186..37473)
                     /locus_tag="JN749_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HAY7367269.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            37633..37890
                     /gene="grxA"
                     /locus_tag="JN749_000762"
     CDS             37633..37890
                     /gene="grxA"
                     /locus_tag="JN749_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HAY7367270.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(37920..38297)
                     /locus_tag="JN749_000763"
     CDS             complement(37920..38297)
                     /locus_tag="JN749_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367271.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            38567..40252
                     /locus_tag="JN749_000764"
     CDS             38567..40252
                     /locus_tag="JN749_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HAY7367272.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            complement(40427..40963)
                     /locus_tag="JN749_000765"
     CDS             complement(40427..40963)
                     /locus_tag="JN749_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7367273.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            41047..42255
                     /locus_tag="JN749_000766"
     CDS             41047..42255
                     /locus_tag="JN749_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367274.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            42255..43070
                     /gene="ybjI"
                     /locus_tag="JN749_000767"
     CDS             42255..43070
                     /gene="ybjI"
                     /locus_tag="JN749_000767"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HAY7367275.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            43156..43441
                     /locus_tag="JN749_000768"
                     /pseudo
     CDS             43156..43441
                     /locus_tag="JN749_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(43482..>44034)
                     /gene="mdfA"
                     /locus_tag="JN749_000769"
     CDS             complement(43482..>44034)
                     /gene="mdfA"
                     /locus_tag="JN749_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7367276.1"
                     /translation="LGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAG
                     NLLLARLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGL
                     ANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVN
                     GILWLSLMVIFLKDKQMGNSHEG"
BASE COUNT        10779 a        11480 c        10806 g        10969 t
ORIGIN      
        1 atttaaagaa gagctcgtac gaaagtgcga gctttttttt cgcattttat ctgtctgtac
       61 ggcagtgaac atggtgggcg ggtggagtat gttacctgtg aatttctaag ctgcctgtac
      121 ggcagtgaac gcgttcgcaa aggttttacc gcaccccgtc gatttctaag ctgcctgtac
      181 ggcagtgaac tcaaatacta cacgtttagc ccgttgttaa taaagcaaaa ccatcccatt
      241 ctcctgcagc accctttttt atccttacct tccagcagca ctaattatca acaacttacc
      301 ttcacctcca aaaatagggt tttcaccgtt ccaccccata aataaaaagt ccgtaacccc
      361 tgacgagatt acggacttca ccaacctacc tgacaatcag attaatgcgc tgcttccgcc
      421 ttgtgctttt gtgcactctg gaatccgtaa gtcagctcat ttttctcttt atccagcgca
      481 accgtcacct gaccgccgtc caccagcgaa ccaaacagca gttcgttggc gagcggtttt
      541 ttcaggttgt cctggatgac acgcgccatc ggacgtgcgc ccattgcccg gtcgtaacct
      601 ttctcggcca gccagttacg cgcttcctgg ctcacttcca gagaaacacc tttctgatcc
      661 agctgaacct gcaactcgac gatgaattta tccaccacct gatggatcac gtcagttgac
      721 agatgatcga accagataat gttgtcgaga cggttacgga attccggtgt aaagatcttc
      781 ttgatctctt ccatcgcatc ggtgctgtta tcctggtgga taagaccaat ggatttgcgc
      841 tcagtttccc gtaccccggc gttagtggtc atcaccagca ccacgttacg gaagtccgct
      901 ttgcgtccgt tgttatcggt cagcgtaccg ttgtccatca cctgcaacag aatattgaac
      961 acgtccggat gcgctttctc gatttcgtcc agcagcagca ccgcatgtgg atgcttgatt
     1021 accgcatcgg tcagcaaacc gccctgatca aaaccaacgt atcccggagg cgcaccaatc
     1081 agacggctga cggtatggcg ttccatatac tcggacatat caaagcgcag aagctcaatg
     1141 cccaacgctt tcgaaagctg taccgtcacc tctgttttcc cgaccccggt aggaccggca
     1201 aacaggaacg aaccgaccgg tttatgttcg tgacctaaac ctgcacgcgc catcttaatg
     1261 gcttcagtca gcgcctcaat ggctttatcc tggccgaaga ccagcatttt caggcgatcg
     1321 ccgaggtttt tcagggtatc gcggtcactc tgagaaacac tcttctctgg aatgcgtgca
     1381 atacgggcca ccacggactc aatatccgcc acattaacgg ttttcttgcg tttgcttacc
     1441 ggcatcagac gtgcgcgagc gcccgcttcg tcgataacat caatggcttt atccggcaga
     1501 tgacgatcgt taatgtattt caccgccagc tctaccgccg cacgcaccgc ttttgcggta
     1561 taacgcacgt cgtggtgcgc ttcatacttc ggtttcaggc cattgatgat ttgaacagtt
     1621 tcttcgatcg acggttcagt aatatcaatt ttctggaagc gacgcgccag agcacggtct
     1681 ttctcgaaaa tgttgctgaa ctcctgatag gttgtcgaac caattacgcg aattttaccg
     1741 ctggagagca acggtttgat taggttagcc gcatcgacct gaccaccaga cgctgcaccc
     1801 gcaccgataa tggtgtggat ctcatcaata aacaggatgc tgttagtgtc ctgctccagc
     1861 tgcttgagca acgctttaaa acgtttttca aagtcgccgc gatattttgt gcccgctaac
     1921 agagaaccga tatcgagaga gtaaatcgta cagtcagcca tcacttccgg cacatcaccc
     1981 tgaacaattc gccaggcaag accttctgca atcgcggttt taccgacacc agattccccc
     2041 accagcagcg ggttgttttt acggcgacgg cagagaacct gaatagcacg ctccagctct
     2101 ttctcacgac caattagtgg gtcgattccg cccacacgcg caagctggtt caggttcgtc
     2161 gtgaaattct ccatacgttc ctccccacca gcttgttctt cgctgtttgg ctggctgcca
     2221 ggatcagaag actgtgtcgg ctcgtcttta cgcgtgccat gagagataaa gttcaccaca
     2281 tcgagacggc tgacttcatg tttacgcaac agatatgccg cctgcgactc ctgttcgctg
     2341 aagatagcga ccagaacgtt tgcaccggta acctcattgc gaccggagga ctggacatgg
     2401 aagaccgcac gttgcagtac acgctgaaaa ctcagcgtcg gctgtgtgtc gcgctcctct
     2461 tcactggcag gcagaacggg tgtggtttgt tcaataaagg cttccagttc ctgacggagc
     2521 gcaaccaaat ccacagaaca cgcttccagc gcctcccggg cagatgggtt actgagcagc
     2581 gccagtaaca agtgctcgac ggtcataaac tcatgacggt gctcgcgcgc tctggcgaaa
     2641 gccatattta aactgagttc cagttcttga ttgagcatag gcacctcccc caatttttat
     2701 gcctgcattc aggctttttc tagcgtacac agcaatggat gctcattctc cctcgcgtac
     2761 ttgttcacca tcgccacttt ggtttctgca acctcggcgg taaagactcc gcaaatggcc
     2821 ttcccctggt agtgaacagc gagcatcaat tgcgttgcac gttctacatc ataagaaaag
     2881 aatttttgta acacgtcaat aacaaactcc atcggagtgt aatcatcatt gactaatatc
     2941 actttataca tagatggcgg ttttagcgcg tcgcgaactt tttcttccgc cagttgatca
     3001 aagtccagcc agtcgttcgt tttacccatt gtcagttatc atcttcggtt acggttatcg
     3061 gcagaacgcc ctgccgctga caagagctta tttgcctgac caatctactc cacatgatag
     3121 ataactatca tctatcgttg gcatcagcga catctgtcac attcctgtca atagcgttaa
     3181 ctgcttcaaa tttttgattc atacctgccc cctggcccct gtcaaatgct tgacggctcg
     3241 ccctaattct ctaaattgta tttctagagt tggcgaggtt ttgaacagcc ccctctctga
     3301 ccccggttta ttccatctta cttgtataag atttgcgaag gatgtcgaag catggaaaag
     3361 ggtactgtta agtggttcaa caatgccaaa ggttttggtt tcatctgccc tgaaggcggc
     3421 ggcgaagata ttttcgctca ttattccacc attcagatgg atggttacag aacgctaaaa
     3481 gctggacaat ccgttcagtt tgatgtccac caggggccaa aaggcaatca cgccagtgtt
     3541 attgtgcccg tcgaagtaga agcggcagtc gcatagctct tctgtctcat tgtgtacatc
     3601 ctaaaggcaa aatgccagcc cgatcggctg gcatttttat ctcaaaaatt actcccgtgc
     3661 cagagcatct actggatcca gtcgtgccgc atttcgtgcg ggtaaccagc caaataaaat
     3721 cccggtggcc gtcgagcaga gaaacgccag cagcagcgcc aacggtgaaa aaccaatctc
     3781 ccagccgggt aagaaaagct gcaaggtgaa agcaattaac agtgacagtg ttattcccaa
     3841 cgcgccaccg accaggcaaa ccagtacggc ttcgatcagg aactgttgca aaacatcgct
     3901 tgctcgcgca cctacagcca tgcgaatgcc aatttcccgc gtccgctcgg tcactgacac
     3961 cagcataata ttcattacac caataccgcc caccaccagc gaaatcaccg ccaccagcgt
     4021 cagaaacagt tgtaaagtac gtgtggtctt ttcaacagtt ttcaagacgc cgtccatgtt
     4081 ccaggtaaag aaatccttct taccgtggcg cagtgaaagt aaacgcgtga gttgctgttc
     4141 cgcctcggcg ctgtcaaatc cttctttcac cctgacagta atggagttaa gccacgactg
     4201 gcccataact cgcccggaca tcgtgctgta aggtagccag acacgcagca ctttactgct
     4261 accaaacatc gactgttttt cttccgccac accaatgact ctggcgggca tattgccgac
     4321 cagaatcacc tcgccaacca catctgcttt atgggggaaa agctggcggc gagtattact
     4381 gtcgagaacc acgacctgcg cacgaccgtt cagctgctcc tgattaaagg tgtttccttc
     4441 actgaaggtc atgccataga cattaaaata atcgccgctc acgccattgg cactggcagc
     4501 aacatcaaca ttgttataac gcaggcgcag gttttgcgag acggcaggtg tggctgaggc
     4561 gacccacggt tgtttttgga tggcgattaa gtcgtcgtac ttcagcgcct gctgatattg
     4621 cggatcgtca tcgccaaaat ctttcccggg atagacatca atagtattcg taccaataga
     4681 acgaatatcc gccagcacca tttgtttggc ggcgtcaccc accacgacaa tggaaaccac
     4741 cgacgcaata ccgataataa tccccagcat ggtcagtaaa gtacgcattt tattcgctgc
     4801 cagcgcccgc catgccatcg tcagcgcctc gttaaaaccg ctgacaaact gccgccagcc
     4861 agacactgta ttgacaacag gctccgtccc gccagcaaca ttcacttttt caatggcggg
     4921 aggattgcgc acaatttcgc cgtcgcgaat ttcgatcacc cgctcggcct gagcagcgac
     4981 ctgcggatcg tgggtgacga taatcaccgt atgcccgcga tcgcgcagct gatgcaggat
     5041 cgccatcacc tcttcgccag aatggctgtc cagtgcgccg gtcggttcat cggcaagaat
     5101 tacctgaccg ccgttcatca atgcccgcgc gatgctgacg cgctgttgct gaccacccga
     5161 aagctgtgcc ggataatact ctgtacggtc ttccagcccc agccgttgca gcaactcctg
     5221 ggcacgaagc agtcgctgtt tccgctcaag accggcatag acggcgggta cttcaacgtt
     5281 ctgctcggcg gttaaatgcg aaagcaaatg gtaacgctgg aaaataaagc cgaaatgctc
     5341 gcggcgcagt tgcgccagcg catcggcgtc cagcgtggca atatcctgac cggctacgcg
     5401 ataggtgccg ctggtggcct tatccaggca gccgagaata ttcatcaggg tcgatttacc
     5461 ggaacccgaa gcgccaacaa tcgcgaccat ctcacccgca taaatattga ggctgatgcc
     5521 cttcagcacc tcaacctgct catcaccggc aggatagctg cgacgaatat cctttaattc
     5581 gagcaaaggc gtcattgtgc agctcctggt ttggcctcac caatcaccac ttcatcgccc
     5641 gcttccagcc ctttgacaat ctcaacatcg gtatcgttac gtgcgccaat cgtcacttca
     5701 cgctcgcgtg tttcaccatt acgcaacaat ttgactttat aacgattatc gccaaccgga
     5761 tcgcctaacg ccgacagagg gatcgtcaac acatttttca catcggtgag ctgaatatgc
     5821 acttgcgcgg tcatatccag ccgcagcaaa ccattggggt tgggtacttc aaaacgggcg
     5881 taatagaaaa tagcgtcgtt taccttttcc ggcgtcggta gcacatcctt gatttgcccc
     5941 tcgtagcgcg tcagtggatc gccaagcacc gtaaaccagg ctttttgccc cggcttcagg
     6001 tggattacat ccgcttcaga aacctgcgct tttaccagca tagtgctcat atctgccagc
     6061 gtcagaatgt tcggtgcttg ctgtgcggca atcaccgtct ggccttgcag agtggtgatt
     6121 tgcgtcactt ccccgcccat cggggcaacg atacgggtgt aatcgagatt ggttttagcc
     6181 gtatcgagag aggcctgatt gcgcttgatt tgcgcgtcaa tggtgccaat ttgcgcctgt
     6241 ttcacagcca tctccgtcgc ggcggtatcg agatcctgca gtgaaaccgc ttgcgtttgt
     6301 gccagacgtt gctgacggga atacgtcacc cgcgccagtt tcagctccgc ttccgcctgc
     6361 tgccgctgcg cgcgtagctc catcagcgtt gcttcgacct ccttgatctg gttttcagcc
     6421 tgttcaggat caatgacccc taaaagctgg tcttttttta ctttatcgcc aatcgccacc
     6481 gacagcgttt tcaactgacc gctgacctgc gcgcctacgt caaccttacg cagcgcgtcc
     6541 agctttccgg tcgccagcac gctttgctgt aaatcaccgg ggcgcacaat cagtgtctga
     6601 taagtcggca cgggtgcgtt aagaattctc cataacgtaa tcagtccggc gatgactatc
     6661 accagcgcaa taacgtaacg cttcttcacg gttttccgct ttttcataaa tattctgaat
     6721 actccacatg ccaatccggg tcattaactt caacgaaata tcaacaaaac cctaaaattt
     6781 acgttagtaa tcacactatt gatgtttggt taagatacgc aatggtgaaa tcgacgtcat
     6841 gtataaccat tgatttcatg aatatgtcgc agctaactga acggaccttt acgccatctg
     6901 aatctctcag cagcctgtca ctttttctta gtctggcacg tggacagtgt cggccgggta
     6961 aattctggca tcgctgtagt tttcgccaga aatttttgct gcgctcgttg attatgccgc
     7021 gtttaagcgt tgagtggatg aacgaacttt cccactggcc taatctcaat gtgttgttaa
     7081 cgcgccagcc gcgactgcct gtgcgtctgc atcgccctta ccttgcggcg aatcttagcc
     7141 gtaagcaatt gctggaggcg ttacgttacc attatgcgtt actccgcggg tgtatgtcgg
     7201 cggaagaatt cagcttatat ttgaataccc ccgggctgca actggctaaa ctggaaggca
     7261 aaaacggtga gcagttcacg cttgagctga ccatgatgat ctcaatggat aaagaaggtg
     7321 acagcacaat cctgttccgc aacagcgaag gtattcctct ggcagagatc acgtttaccc
     7381 tgtgtgaata tcaggggaaa agaacgatgt ttattggcgg actgcaaggc gcaaaatggg
     7441 aaattccaca tcaggaaatc cagaatgcga cgaaagcctg ccacgggcta tttcccaaac
     7501 gcctcgtgat ggaagcggcc tgtctgtttg cccaacgttt gcaggtagag cagattattg
     7561 ccgtcagcaa tgaaacgcat atttaccgca gcctgcgtta tcgcgataaa gaaggcaaga
     7621 tccatgctga ttacaacgcg ttctgggagt cggttggcgg cgtatgtgat gctgaacgcc
     7681 attaccgcct tccagcacag atagcacgaa aagagattgc cgaaatcgcc agtaaaaaac
     7741 gggctgaata ccgtcggcgc tatgagatgc tcgacgctat tcagccacaa atggccacga
     7801 tgtttcgcgg ttaatccgcg cgaccgcgcg ccagccacag cacgcgtgag aacatttttt
     7861 tcagcagcgt tggtacggag tccacaccac gacgtcctgc ctccattgcc acttcaatgg
     7921 caagatcggg tttggaagag cgatggatcg ctttcgagat aattttgcgt agattcatcg
     7981 gtacattttc cgggatttgc gccacgcggt ggaacacatc ggaaaatccc tggcgataca
     8041 taaaatgttc catatccagc gccggtaacg ccgttaaatg ttctcgttcg gcttcccgat
     8101 cgttattcaa caggctgcgt accgtagcgg catatttctt ccctgcttca tcgccatcga
     8161 ccagtacatg ccattcaatc cccattcggc gggcaaattt aaccagtggc tttagcccgg
     8221 actgggcaaa ctcaatgacc ttgatccctt cggcatcaaa atgatgtccg cactgacgcg
     8281 ccagttcatt gataacccag gtttccgttt ccccttccac cagcaaccag cagcgagcaa
     8341 acagcgatga cggacgatta aaacgaatgt gaaaagagat gcggcggctg tcttcggtgc
     8401 tcaagccact tggccccaga cgccaggcgg caacacgaga agattcacgc accaggcggc
     8461 aaacatgttc cactggagtt aacgaaagca gctcgccaga gttggtggta gcaatacgct
     8521 gtaaaggcaa aagattcaac agatgccagg caacggaaag cataatcgga tgcaggcggg
     8581 tttctggatc ttcaattagc aataacggac gcgcgtcttt atccagtcgc aatgtgcctt
     8641 ttgcctgcaa taaagtagca aacaggccga gcaaaataac ccgatacgag cgccctccag
     8701 gtcggtcaat catccggttg atgatgtcca gatagcgcca gcttcgttgc tcattgctgg
     8761 cccgccgccg cattaaacga tatcgcgcct gtccagcccc ctgctcagag aaataatgct
     8821 caagcaactg caccattgcg gaaagtccct gacgaatctg tccatcagag agattttgcg
     8881 gatgtgagga caactcacgg gcgaggaaat cgagctggcg cgcggtgact tccacattgg
     8941 ggacatttgg caccgtgccg ttacgaatac ggcgcataaa acgggcatca cgcaagcgca
     9001 gcaccggcat taaacgcacc agatggcgtg cctgatcgtt aatatcctca acatcaatcg
     9061 gatgtccgtc tttatcgaga aaactgcgca gtgtcatcac gctgccgtct tccgcactct
     9121 ccccttccag acgataaaaa atacggtgat agccatcggt gcatggcgtc cagcacgctt
     9181 ccagcggccg ataacggcga acccgatgtc ggcctggcag cgattcgcgg aaggtcaaaa
     9241 taatatgcag atgatgttct cgcccgttga tatctcccgg cgggaaccag aaatcgtcgc
     9301 gctcaaaatg gtagagatct gattctggcg atagcagcag ggttaaggcg tccagcaagc
     9361 tggatttacc ccacgcgttc tccccaatca ggacgttgtt ttgttcgagc atcaacgaca
     9421 aacggttgat accgcgaaaa cccacaattt caacgcgctc aagaatcata tcccctccag
     9481 aaatatgtga cttatccttt acgctggcag tataacggga aaggccttgc taatacgtga
     9541 tgacatcaca tttaagcatg gcaattgtct gaaaaatgag cgaataatcg cgttatactc
     9601 cctcttgctg ataagcccgc aaaaacaaca aaatcaaata attagcatga taaattattt
     9661 tttattacgc ctaaaggatt aaatcttttg cttgcactac actgtcaatc agacaaatga
     9721 ctatatgggg caacccgctg ttgccctaaa tcaaatttac tggatttatt tatctggtga
     9781 atgactcgcc ttcgttttta aaatagccgc gcattatttc cgtcgtcatt ttttacgacg
     9841 accctataaa acgaccctat ttttcacagg gtcaattttt aattgaggtg gatatgttca
     9901 gaaaattagc agctgaatgt tttggtactt tctggcttgt ttttggtggc tgtggtagtg
     9961 ctgtactggc cgcaggcttc ccggaattag gcattggttt tgccggcgtg gcgctggcat
    10021 ttggtttgac cgttctgacg atggccttcg ctgttggtca tatttctggt ggtcatttta
    10081 acccggcggt cactattggt ttatgggctg gcggacgttt tccggcaaaa gaagtcgttg
    10141 gctacgtgat tgcccaggtt gtcggcggta ttgttgcagc ggcgctgctg tatttaattg
    10201 tcagtggtaa aacgggtttt gacgcggcag ccagcggttt tgcttctaac ggttatggcg
    10261 agcattcacc aggcggttat tccatgcttt ccgcgctggt agttgaactg gtattgagtg
    10321 cgggcttcct gttagtgatc cacggtgcaa ccgacaaatt cgctccggca ggttttgcgc
    10381 cgatcgctat tggtctggcc ttaaccctga ttcacttaat cagtattccg gtgactaaca
    10441 cttctgttaa cccggcgcgc agcaccgcgg ttgctatctt ccagggcggc tgggcattag
    10501 aacaactgtg gttcttctgg gtggtgccaa ttgtcggcgg cattatcggt ggtctgattt
    10561 accggactct gctggaaaag cgtgattaat tagcattggc attttatctg ccctggttgt
    10621 tttatcttcg acattacaaa ccgcacgggt attggtggcg tcaaaatcgc tgaagcgttc
    10681 cccgaaggcc ggggcagccc acatggatgt gggctgaggg cgcgtcttac aggacgttac
    10741 ctcgcgcccg acctggttgc cgaaggggat aagctgaagg taccgcgcag cggcgatttt
    10801 gttcgccaga gcccgggggt gcagggggcg gcggcgactg gctgccccct gcgcgctccc
    10861 tccgccagtg gctttatatg gcattgcaca tgaaaggagc gcaacaatgc taaacactca
    10921 gataggatta aaaatccccc tctgatgcat aacatttcct tttaattgca actttactcg
    10981 tccttccgct ttcagtagtg tgtcatcgcg catttcgact tcttctttgc aaggactgga
    11041 ttttcatgtt ttctgggctg ttaatcattc tggttcccct gattgtgggt tacctcattc
    11101 cgcttcgcca acaagctgcg ttaaaagtta ttaatcagct attaagctgg atggtttacc
    11161 ttattctctt ttttatgggt atcagtctgg cgtttctcga taacctcgcc agtaacctgt
    11221 tggcgattct gcattattct gccgtcagta ttaccgttat tttactgtgt aatattgccg
    11281 ccctgatgtg gctggagcga ggcctgccgt ggcgcaacca ccatcagcaa gaaaaactcc
    11341 cgtcgcgtat tgcgatggcg ctggagtcgc taaaactgtg cggcgtagta gtgattggtt
    11401 ttgccattgg tctgagtgga ctggctttct tacaacacgc gaccgaagcc agtgaataca
    11461 cgttaatttt gctacttttc ctcgttggta ttcagttgcg caataatggc atgaccttaa
    11521 agcagattgt ccttaatcgc cggggaatga ttgtcgccgt ggtggtggtt gtcagttcat
    11581 taattggtgg tttaattaac gcctttattc ttgatctccc catcaatacc gcgctggcaa
    11641 tggcctccgg tttcggctgg tattctcttt ccggtatttt attgaccgaa tcttttggtc
    11701 cggtaatcgg gagcgcggcg ttttttaatg atctggcccg tgaactgatt gctattatgt
    11761 tgatccctgg gctgattcgc cgcagccgct ctactgcact gggcttatgc ggtgccacat
    11821 caatggattt caccctgccc gttcttcaac gtactggcgg gctggatatg gtcccggcgg
    11881 caattgttca cggttttatt cttagcctgt tagtgccgat cctcatcgcc tttttctctg
    11941 cgtaatacct ctctggcggt agatccctgc cgccaaaatt gcgctaaatc aatctccctt
    12001 aaagttgcat gaaaaatccc ttttatcccc gcgttaagcg tcttaacctt aaacatgtat
    12061 attaaatata actttaaaag gtgtgatcat gttttgtgtg caatgtgaac aaactatccg
    12121 tactccggca ggaaacggct gctcatacgc gcaggggatg tgtggtaaaa cggcggaaac
    12181 ttctgacctt caggatttac tcatcgcggc gctgcaaggg ctttctgcct gggcggtaaa
    12241 agcgcgtgaa tacggcatca tcaaccacga tgttgacagc ttcgcgccac gtgccttttt
    12301 ctcaaccctg accaacgtta acttcgattc tccgcgtatt gtcggctacg ctcgtgaagc
    12361 gattgccttg cgcgaggcgc tgaaagcaca atgcctggct gtagatgcca acgcgcgcgt
    12421 cgataacccg atggctgacc tgcaactggt gagcgatgat ctcggcgagc tgcaacgtca
    12481 ggcagcagaa tttaccccta acaaagataa agcggcgatt ggcgaaaaca ttctcggcct
    12541 gcgtctgctg tgcctgtatg gcctgaaagg tgcggcggcc tatatggaac acgcgcatgt
    12601 tctcggtcaa tacgacaacg atatttatgc ccagtaccat aaaatcatgg cgtggctggg
    12661 gacctggcct gccgatatga acgcgcttct tgagtgttca atggaaatcg gccagatgaa
    12721 cttcaaagtg atgagcattc tggatgcagg cgaaaccggt aaatacggtc acccgacgcc
    12781 aacccaggtc aacgtcaaag cgacggcggg taaatgcatt ctgatttccg gtcacgatct
    12841 caaagatctc tacaacctgc tggaacagac cgaaggcacg ggcgttaatg tctacaccca
    12901 cggcgaaatg ctgcctgcgc atggctaccc ggagctgcgt aaattcaagc atctggtcgg
    12961 taactacggc agcggctggc agaatcagca agtggagttc gctcgtttcc ctggccccat
    13021 cgtgatgacc tcgaactgca tcatcgaccc aaccgtaggc gcttatgacg atcgtatctg
    13081 gacccgcagc atcgttggct ggcctggcgt gcgtcatctg gatggcgaag atttctctgc
    13141 ggtcatcgcc caggcgcaac aaatggcggg cttcccgtac agcgaaattc ctcacctgat
    13201 caccgtgggc tttggtcgcc agacgctgct tggtgcagcc gatacgctga ttgatctggt
    13261 tagccgtgaa aaactgcgtc atatcttcct gcttggtggc tgtgacggcg cacgcggcga
    13321 gcgccactac ttcaccgatt tcgccaccag cgtgccggat gactgcctga tcctgaccct
    13381 cgcctgtggt aaatatcgct ttaacaaact tgagtttggc gatatcgaag gtctgccgcg
    13441 tctggtagat gcaggtcaat gtaacgatgc ttactcagca attattctgg ctgtcactct
    13501 ggcagagaaa ctgggctgtg gtgtgaacga tctgccgctg tcgctggtgc tctcctggtt
    13561 tgaacagaaa gcgattgtca ttctgctgac gctgctttct ctgggcgtga aaaatatcgt
    13621 caccggcccg actgcaccta gtttcctgac accagacctg ctggcggtgc tgaacgagaa
    13681 attcggcctg cgttctatca ccactgttga agaagacatg aagcaactgt tgagcgcgta
    13741 aggagtttgt cgatgacgat gccaacgaat caatgcccgt ggcggatgca ggttcatcac
    13801 attacgcaag aaacgccgga tgtgtggacg atttccctga tttgccacga tcactaccca
    13861 tatcgcgccg ggcaatatgc actggtcagc gtgcgaaact cagcggaaac gctgcgtgct
    13921 tacaccattt cctccacgcc aggcgtgagt gaatatatca ccctgaccgt gcggcggatt
    13981 gatgacggtg tcggctccca gtggctgacg cgcgatgtaa aacgcggtga ttatctctgg
    14041 ctttcggacg cgatggggga atttacctgc gacgataaag cagaagataa attcctgttg
    14101 ctggcggcag gctgcggcgt cacgccgatt atgtcgatgc gtcgctggct tgcgaagaac
    14161 cgtccacagg ccgatgtgcg ggtgatctac aacgtgcgta cgccgcagga tgttattttc
    14221 gccgatgagt ggcgtaacta tccggtaacg ctggtggcag aaaataacgt taccgaaggc
    14281 tttatcgctg gtcgtctcac tcgcgaactg ctggcagatg tacctgactt agcttcacgt
    14341 accgtgatga cctgcggccc ggctccgtat atggattggg tagagcagga agtgaaagcg
    14401 ctcggcgtga cgcgtttctt taaagagaaa ttcttcaccc cagtagcaga agcggcgacc
    14461 agcggtctga aattcaccaa actgcaaccg gcacgagaat tttacgcccc ggttggcacc
    14521 acgctactgg aggcgctgga aagcaataac gttccggttg tcgctgcctg ccgtgcgggt
    14581 gtttgcggct gctgtaagac aaaagtggtt tccggtgaat atacggtgag cagtacaatg
    14641 acgctgaccg acgccgaaat cgctgaaggt tacgtactgg cctgctcctg ccatccgcag
    14701 ggggatttgg ttctcgcata atcgccttat gcccgatgat attcctttca tcgggctatt
    14761 taaccgttag tgcctccttt ctctcccatc ccttccccct ccgtcagatg aactaaactt
    14821 gttaccgtta tcacattcag gagatggaga accatgaaac aaacggttgc agcttatatc
    14881 gccaaaacac tcgaatcggc aggcgtgaaa cgcatctggg gagtcacggg cgactctctg
    14941 aacggtctta gcgacagtct taatcgcatg ggcaccatcg agtggatgtc cacccgccat
    15001 gaagaagtgg cggcgtttgc cgctggcgct gaagcacaac tttcaggaga actggcggtc
    15061 tgtgccggat cgtgcggccc aggcaacctg cacttgatca acggcctatt cgattgccac
    15121 cgcaataacg ttccggtact ggcgattgcc gctcatattc cctccagcga aattggcagc
    15181 ggctatttcc aggaaaccca cccacaagag ctattccgcg aatgtagtca ctattgcgag
    15241 ctggtttcca gcccggagca gatcccacaa gtgctggcaa ttgctatgcg caaagcggtg
    15301 cttaaccgtg gcgtttccgt tgttgtgtta ccgggcgacg tggcgttaaa acctgcgcca
    15361 gaaggggcaa ccacgcactg gtatcatgcg ccacagccag tcgtgacgcc ggaagaagaa
    15421 gagttacgca aactggcgca actgctgcgt tattccagca atatcgccct gatgtgtggc
    15481 agcggctgcg cgggggcgca taaagagtta gttgagtttg ccgggaaaat taaagcacct
    15541 attgttcatg ccctacgcgg taaagagcat gtcgaatacg ataatccgta tgatgttgga
    15601 atgaccgggt taatcggatt ctcgtcaggt ttccatacca tgatgaacgc cgacacgtta
    15661 gtgctactcg gtactcagtt cccctaccgt gcgttctacc cgaccgatgc caaaattatt
    15721 cagattgata ttaatccagc cagcatcggc gcacatagca aggtagatat ggcgctggtc
    15781 ggcgatatca aatcaaccct gcgcgcattg ctgccactgg tggaagaaaa aaccgatcgc
    15841 aagtttctgg ataaagcgct ggaagattac cgcgatgccc gcaaagggct ggacgattta
    15901 gctaaaccga gcgagaaagc cattcacccg caatatctgg cgcagcaaat tagtcatttt
    15961 gccgccgatg acgccatctt tacctgtgac gtcggcacgc caacggtatg ggcggcacgt
    16021 tatctgaaaa tgaacggcaa gcgtcgcctg ttaggttcgt ttaaccacgg ttcgatggct
    16081 aacgccatgc cgcaggcgtt gggtgcgcag gcaaccgagc cggaacgtca ggtggtcgcc
    16141 atgtgcggcg atggcggttt tagcatgttg atgggcgatt tcctctcagt agtgcagatg
    16201 aaactgccgg tgaaaattat cgtctttaat aacagcgtgc tgggctttgt ggcgatggag
    16261 atgaaggccg gaggttacct gacagacggt actgagctgc acgacaccaa ctttgcccgc
    16321 attgccgaag cgtgcggcat tacgggtatc cgtgtagaaa aagcctctga agttgatgaa
    16381 gccctgcaac gcgccttctc tatcgacggt ccggtgttgg tggatgtggt ggtcgccaaa
    16441 gaagaattag ccattccacc gcagatcaaa ctcgaacagg ccaaaggttt tagcctgtat
    16501 atgttgcgcg caattatcag cggacgcggc gatgaagtga tcgaactggc gaaaacgaac
    16561 tggctaaggt aaaaagggtg gcatttcccg tcataataag gacatgccat gattgattta
    16621 cgcagtgata ccgttacccg accgagccgc gccatgctcg aagcgatgat ggccgccccg
    16681 gttggggacg acgtttacgg agacgaccct accgttaatg ctctgcagga ctatgcagca
    16741 gagctttccg gtaaagaagc cgccattttt ctgcctaccg gcactcaggc caacctggtc
    16801 gctctgctca gtcactgcga acgtggcgaa gagtatattg tcggtcaggc cgcgcataac
    16861 tatctgtttg aagccggtgg cgcagcggtg ctgggcagta ttcagccgca acccatcgac
    16921 gcggctgccg acggcacgct accgctggat aaagtggcga tgaaaatcaa acccgacgat
    16981 atccatttcg cccgcaccaa attactcagt ctggaaaaca cccacaacgg caaagtgctg
    17041 ccgcgtgaat acctgaaaga agcatgggaa tttacccgcg agcgcaatct ggcgctgcat
    17101 gttgacggtg cgcacatctt taatgccgtg gtggcttacg gctgcgaact gaaagagatc
    17161 acgcaatatt gtgattcgtt caccatttgc ctgtcgaaag gtcttgggac gccagtcggt
    17221 tcattactcg tcggtaatcg tgattacatt aaacgtgcca tccgctggcg gaaaatggcc
    17281 ggtggcggga tgcgtcagtc cgggattctg gctgccgccg ggatgtatgc cctgaaaaat
    17341 aacgtcgcac ggttgcagga agatcacgac aacgccgcct ggatggcgga gcaactgcgt
    17401 gaagcaggcg cggatgtgat gcgtcaggac actaatatgc tgtttgttcg cgtcggggaa
    17461 gaaaatgctg ccgcgttagg cgaatacatg aaagcgagaa acgtgctgat taacgcctcg
    17521 ccgattgtcc gcctggtgac gcatcttgac gtctcgcgcg aacaactggc ggaagtcgcc
    17581 gcccactggc gcgcattcct ggcgcgttaa ggagagagac gtgccgcaac gcattttagt
    17641 tctcggtgcc agtggctaca ttggtcagca tctggtgcgc acactcagcc agcaagggca
    17701 tcagatcctg gcggcggcac gtcatgtcga caggcttgca aagctgcaac tggcaaacgt
    17761 cagttgccat aaagtcgatc tcagctggcc ggataacctt cctgccctgt tgcaggatat
    17821 cgataccgtc tattttctgg tgcacagcat gggcgaaggc ggcgatttta tcgctcagga
    17881 gcgccaggtg gctctcaacg tccgcgatgc gctacgtgaa gtaccagtta agcaattaat
    17941 ctttctcagt tcgttgcagg ccccgccaca tgagcagtcg gatcatctgc gagctcgtca
    18001 ggctacggcg gacattcttc gtgaagcgaa tgtaccagtg accgaacttc gggccggaat
    18061 tatcgttggc gcaggttcag cggcgttcga agtcatgcgc gatatggtct acaacctgcc
    18121 agtgttaacg ccgccacgct gggtacgttc acgcaccacg cccatcgcgc tggaaaactt
    18181 gctgcactat ctggtggcgc tgttagacca tccagccaac aaacaccgca tcttcgaagc
    18241 cgccggacca gaggtgctca gttatcagca acagtttgaa cattttatgg cggtgagcgg
    18301 taagcgccgc tggttgatcc ccatccccct ccccacccgc tggatttcgg tgtggtttct
    18361 caatgtgatt acttccgtac cgcccaccac cgccagggcg ttgattcagg ggctgaaaca
    18421 cgatctgctg gcagatgata ccgcgctacg tgcactcatc ccacaacggc tgatcgcttt
    18481 cgatgacgcg gtacgtagca cgttgaaaga ggaagaaaaa ctggtcaact ccagcgactg
    18541 gggctacgac gctcaggcct ttgcccgctg gcgaccggag tacggttatt tcgccaaaca
    18601 ggcggggttt accgttaaaa cgtccgccag ccttgcggct ttatggctgg tggtgaacca
    18661 aatcggcggt aaagagcgtt atttctttgg caatattttg tggcagacac gggcgttgat
    18721 ggaccgcgcg atcggtcata atctggcgaa aggccgcccg gagcgcgaat atttacagac
    18781 cggcgatgcg gtggatagct ggaaagtgat tgtcgttgaa ccgaaaaagc aacttacgtt
    18841 gttatttggc atgaaagcgc cggggctggg acgactgtgt tttaccctgg aagataaagg
    18901 tgactatcgt actatcgatg tccgcgcttt ctggcatccg cacggtatgc cggggctgtt
    18961 ttactggtta ttgatgatcc ccgcgcatct gtttattttt cgcggaatgg caaaacaaat
    19021 cgccagactg gcagaacaaa gcacagatta atcgacaaaa cctgcgttat tctttcatgt
    19081 ttcccattgc atcatggggg aaatcacgga agaatgcgca cgtcattttc tcaacaacag
    19141 tggattgata tgaaggtact ggttaccggc gccaccagcg gcttaggtcg aaacgcggta
    19201 gagtttttat gccagaaagg catcagcgtg cgtgcgagcg gtcgcaacga agcaatgggt
    19261 aaattgctgg aaaaaatggg cgcagagttt gttctgacgg atctgaccga gctggtttca
    19321 tcacaagcta aagtgatgct cgcgggcatt gatacgctgt ggcactgctc cagctttacc
    19381 tcaccctggg ggacacaaca ggctttcgat ctggctaacg ttcgcgccac tcgccgcctg
    19441 ggtgaatggg ctgtcgcctg gggtgtgcgt aactttattc atatctcttc cccctccctg
    19501 tacttcgatt atcaccacca tcgcgatatt aaagaagatt ttcgccctca ccgcttcgcc
    19561 aacgagtttg cccgcagcaa agcagccagc gaagaagtga tcaatatgct ttcgcaggcg
    19621 aatccacaaa cgcgctttac tattctgcgc ccacaaagtc tgttcggacc gcacgataaa
    19681 gtctttattc cccgtctggc gcatatgatg caccactacg gcagtattct gttaccgcat
    19741 ggcggcagtg cgctggtcga tatgacctac tatgaaaatg ccgtgcacgc aatgtggctg
    19801 gcaagccagg aagcctgcga taagctacct tccgggcgtg tgtacaacat caccaacggc
    19861 gagcatcgca cactgcgcag catcgtgcag aagctgatcg acgagttgaa tattgactgt
    19921 cgtattcgtt ccgtccctta cccgatgctg gatatgatcg cccacagcat ggagcgttta
    19981 ggccgcaagt cagcaaaaga gccgccgctg acccactacg gcgtctccaa gttgaatttt
    20041 gactttacgc tggatattac gcgtgcgcag gaagagttag gttatcagcc ggtcatcacc
    20101 ctggatgaag gcatcgagaa aactgccgcc tggctacgcg accacggaaa actgccgcgc
    20161 taatcctgcc cgtatttctc cagcaatgct tcggcaatcg cctgagtttc tgcatccgct
    20221 tcgccgttat ataacgtcgg gcggaaatgc atctggaatg ccataatcac gcgccgctgc
    20281 tcgcgcggtg tcatatcagg ttttacgtca taaccgtagc gcgccaaaag ctccagcaat
    20341 gacgcggtat ctaccggtgt gtgcggcgcg cgcccggcaa ggtaaaagtt cacccgctgc
    20401 gcatccggcc aggcaccaat cccctgctgc gccagttgct gccagggaaa taatggcccc
    20461 ggatcgtctt tgcgctgcgg tgcgatatcc gcatgtgcca ctacgttttc cggcttgatg
    20521 tgataacggg caataatatc tttcgccagc ggaataagcg cctgaatctg tgcctgttca
    20581 aacggggcaa aatatttcac tccggatgat ttttgccagc cacggttttc cagctcaatg
    20641 ccaatagagg tgtcgttaag gcgcgttgcc ccgcgccagg cgctaatccc cgcatgccag
    20701 gccagttctt gttccggcac cagttgccag atgcgcggtt taccgttgta tcgcggtggc
    20761 accgcaggga tcagataatg cgagctgacc tgcttatcag tcagtgtcgc cagcgagcta
    20821 tcaaaatcat ctgcggtgta gtggatcacc agcactttaa tgcgcggata cgccgcctgc
    20881 gcctggtgtc gggtatcaag ctgatatccc tctttctcga caatgccttt ttcgcctgca
    20941 caccctgcca gtaacagagc ggcagcgacc agccagaaga ctcttctcat cgacgcgttt
    21001 tcaccgccgt accgctgacg ctaaccatca gcatactgcc gttttgcccg accgtttcgt
    21061 agtcgatatc aataccaact acggcatcgg cccccagcgc ccgcgcctgg gagcctaatt
    21121 cctcaaaggc gatctcccgt gctttacgca gttctttttc atacgcaccg gaacgtccgc
    21181 caacgatatc gcggataccg gcaaagaaat cacggaaaat attggcacct aaaatcgctt
    21241 cgccggtcac cacaccgcaa tattcaacga tggtctggcc ttccagagtt ggggttgtag
    21301 aaaattgcat gatcgatctc cttgtttgtt aagccttcga tctctaaagc attatcggac
    21361 tgatatgcca tgttgggaag aaggttttga tcagtttagg aaatcatctc attgtgtcaa
    21421 aaacacccac ttaagtcttt acgattatcc gttgcataac ccgatattgg tttcattata
    21481 gggtaccgct tacctgtctt attatgttgc accaacaaca ttaatgagcg gaaagaggca
    21541 acttatattg taaatgactc ctaaagtctt ccccatcttg tgactgaggt ctataccgct
    21601 ggattgcttt ttggtcagcc gccgcaaaac aagaaagtaa taagatctca atgcttttgt
    21661 cattaatata agcgaagatc attcttgcaa actcgtatat aaacgcccga tcctgagcgc
    21721 ttaatagttt aatacaatat ttatttttaa gagagaaaaa taatttcaag gaaagcatcc
    21781 gcagcaacct tcacgttatt cccggcgttc atattatata tatcccttgc acagatagag
    21841 tacagagata gtaaacagcc atacaaataa tcacttatta attcttttgt ccattttaca
    21901 ggcaaccagg gttttatctt gacaccatgc atactacatt catgaaaaag acatttgctg
    21961 agattaactc ttatcatatc aacattgaaa aaattgcagt taataaattt taaagaatga
    22021 gtatttacgt tattaatttt aacagatcta aacgaaatat ttttgaatgc actgtttatt
    22081 acattgctat ttgttatttt aacatcacct aaactgccgt tttcaaaaca acattttttc
    22141 aatatgcttt cattcacaac agtgtgtgat aaatagagat tacttatgtt ttgctttatt
    22201 aatgccacgt cattaaaatg actatattta atgaaacaac catcattgag tagtgtttta
    22261 agattgacta aattacttgc atcttcacgt accatcgtgg caactggaac aggcagagac
    22321 atgaaaactt cttcattcac catcaagtga atcatgcatt gcgttccctt tgtcatatcc
    22381 acacttacgc tacaaatatc gtggtaatgc gaagagtgca tttcaggaaa cagattaaac
    22441 acggtatctt gaaaggcact aaatcgtgtt aataaatcct gataatgaac aggttttgtt
    22501 tcaaaattat catcagcaaa tccaagcact attgcatgct ctacatctaa cgcagtttca
    22561 aatgactctt cttctgtaaa atcattattt aaaacctcac ctgacgtatt atcactgtca
    22621 taaaaagtgc tcatcaatgc gatattgttt gttacagcgt ctttatcgat attctcacta
    22681 atagcatatc gcctgctata atcataaaaa tgaataaatg cgtcctttat aaaggatata
    22741 tattgttctt tgttaaagtc aatacgactg tataactttt caaaaatcac attcgcaatc
    22801 ttactaccaa tggtatacct gtataataat gggtgcgatt caatcgtaaa atacttatct
    22861 aattgttctt gtaatttatt ctcatagtcc agtgggaaaa gaaactgttg tctttcaagt
    22921 tttccattcg atacattacg tattccaata gagattcttt cattacccgc ttttatttca
    22981 acttcgttat cttctgattt acccagatat tttttaacat caatctcgaa agcattgcta
    23041 aaaaatgtat taaattggta attcgctgat ggaatatttt ccgcgagcaa cacatcattt
    23101 tttatgagat cactgtcagc atttattgct aatatcagat gcaagtagat ggtatcccat
    23161 gtagacaact cacccttacg actggaattt aatatcgcga aaaattccct cagtgaatcc
    23221 aggaaattat cactttgatt tagttgtgtg cctggaaatg atgatgcttg atcagatata
    23281 ttgtttatca tattaaaaat catccaagaa aaacgataat ccaccgaact cctcgcgctt
    23341 tataattata aaagcacaac accttttgta ttcatattaa ttaacgaaat actgaagcaa
    23401 tttaatttat tcttcactca aattcattag cttaaattct ttacccacaa ttgtagcata
    23461 aataatatta tcgagagtca aagctgggac tatcaaattt cgctcatttt caaaaataca
    23521 acgtaatttt ggcaatcatc ttaatcgcag gatctcttat cgcatgtgaa aaattttgta
    23581 atgattagac aataaccgca ttcatttatc acgcgttata aatgtgaaac tggcttcttg
    23641 cagatgctct taacctctac aggaggattt ataccaccat ttcagaacta tcatatttac
    23701 cttaaatgat catttcagcc atataacacg ctcaacctca caaatggttt acactgtcta
    23761 ttgtttgggt aatttaagta agtcagctac gagcaacctt gagtgggtag ccagcaatgg
    23821 actcaaaccc aatcaggtgt tcagattgtt tcagaattgt attaaaaaca atgtattaaa
    23881 taatctgata gcgtttggtc gtagtgtgac gcagacagca agctaataag gaaatgatta
    23941 tgcgctaccg ttcactatct ctacttattc catgtgcact gctgctgagt gcctgtacca
    24001 ccgtcacgcc agcttataaa gataacggcc cccgcaccgg ttcctgtgtc cagggagggc
    24061 ctgacagtgt ggctcaacaa ttctacgact accgcattca acatcgcagc aatgacatta
    24121 ccgccctgcg cccctatttg agtgacaaac tggcaaccca acttagcgat gccagccgcg
    24181 ataatagtca tcgtgaattg ttaagcagcg atccgttctc cagtcgaacc accttgccag
    24241 acagcgccca cgttgccagt gcatcgacta tccctaatcg tgatgcccgt aacattccat
    24301 tgcgggtgga tctcaaacag ggtgaccagg gctggcagga tgaagtgtta atgattcagg
    24361 aaggccagtg ttgggtcatc gacgatgtgc gctacttagg cggtagcgtt catgccacgg
    24421 cgggtacgct ccgtcagtcc atcgagaatc gctaacgcct tgatcaacca ataaaccacg
    24481 taaattatcc ggcatttcgt gaggaatgcc gacatttatg ctcaccgacc accgcccccg
    24541 ttattttgtg ctatgtttat tgaataatgc gctttgcttt taacttttaa agcataaata
    24601 ttgcataatt attctgtcaa aggtactatc ttcgacctca atagctatca gactgccagt
    24661 atacgagtgt caatgagtat tcaattaaac ggcattaatt gcttctacgg cgcgcatcag
    24721 gcgctgttcg atatcacgct ggattgccca cagggcgaaa cgctggtgtt acttggcccc
    24781 agcggcgcgg gtaaaagctc gctgctgcgt gtactcaatc tgcttgagat gccgcgctcc
    24841 ggtacgctca acattgcagg caaccatttc gatttcacca aaacaccctc tgacaaagcg
    24901 attcgcgatt tgcgtcgtaa cgttggcatg gtgtttcagc aatacaacct gtggccgcat
    24961 ctgaccgtgc agcaaaacct gattgaagcg ccctgccgtg tactggggtt gagtaaagat
    25021 caggcgctgg cccgtgcaga aaaactgctg gaacgtctgc gtctcaaacc ttatagcgat
    25081 cgttacccgc tgcatctttc tggtggtcag cagcagcgtg ttgctattgc ccgtgcgttg
    25141 atgatggaac cgcaggtact gctgttcgat gaaccgaccg ccgcactgga cccggaaatt
    25201 acggcacaaa tcgtcagcat cattcgtgag ctggcagaaa cgaatattac ccaggtgatc
    25261 gtcacccacg aagttgaagt ggcgcgtaaa accgccagcc gagtggtgta tatggaaaat
    25321 ggtcatatcg tagaacaagg cgacgcgagc tgctttaccg agccgcaaac cgaagcattt
    25381 aaaaactatc tctctcacta agattcggga taacaacaat gaaaaaagtt ctgattgccg
    25441 cgttaattgc aggttttagt ctttccgcca cagctgccga aaccattcgt tttgctaccg
    25501 aagcctccta tcctccgttt gaatcgattg atgcaaacaa ccagatcgtt ggttttgacg
    25561 tcgacctggc acaggcactg tgtaaagaga ttgatgcaac ctgtactttc tctaaccagg
    25621 cgtttgacag cctgatccca agcctgaaat tccgtcgcgt agaagcagtg atggcgggca
    25681 tggatatcac tccggagcgt gaaaagcagg tactgtttac taccccgtac tatgacaact
    25741 ctgccctgtt tgtgggtcag caaggcaaat acaccagtgt tgatcagctg aaaggcaaaa
    25801 aagtcggcgt tcagaacggg acgacacacc agaagttcat tatggataag cacccggaaa
    25861 tcaccaccgt tccgtatgac agctaccaga acgcaaaact ggatctgcaa aacgggcgta
    25921 tcgacagcgt atttggtgac accgcagtgg tcactgagtg gctgaaagat aacccaaaac
    25981 tggcggcagt gggcgataaa gtgaccgata aagattattt cggtactggc ctcggcatcg
    26041 cggtacgtca gggcaacact gaactgcagc agaaactcaa cactgcgctg gaaaaagtga
    26101 agaaagatgg cacttacgaa accatctaca acaaatggtt ccagaagtaa ttcctgatga
    26161 atgaattttt tcctttagca agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat
    26221 tgattgtcgg gctggcgctg gcgatgttct ttgcggtatg ggagtcagcg aaatggcgtc
    26281 ctgtcgcgtg ggcaggttca gcgctggtaa ccattctgcg tggcctgcca gaaattctgg
    26341 tagtgctgtt tatctatttt ggctcctcgc agttactgct gacgctttcg gatggcttca
    26401 ctatcaatct tgggttcgtg cagatcccgg tgcagatgga cattgagaac ttcgacgtga
    26461 gtccgttcct ttgtggtgtc atcgctctgt cactgctgta tgccgcctat gcctcgcaaa
    26521 cgctgcgcgg cgcgttgaaa gctgtaccgg tgggccagtg ggaatctggt caggcgctgg
    26581 ggctgtcgaa atcggctatc tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc
    26641 tgcctggcct cggtaaccag tggctggtgc tgctgaaaga taccgcgctg gtcagtttga
    26701 ttagtgtgaa tgatttaatg ttacaaacca aaagcattgc tactcgtacc caggaaccat
    26761 ttacctggta cattgtggcg gcggcgattt acctggtgat caccctgctc agtcagtaca
    26821 ttctcaaacg cattgacctg cgcgcgacac gttttgagcg gaggcccagc taatgtttga
    26881 gtatttaccc gagctgatga aagggctgca caccagcctg acgctaaccg ttgcctcgct
    26941 gattgtggca ctgattctgg cattgatttt taccatcatc ctgacgctga aaacgccggt
    27001 gctggtgtgg ctggtgcggg gttatatcac gctgtttacc ggtacgccgc tgctggtgca
    27061 gatcttcctg atttattacg ggccgggcca gtttccgacg ttgcaggagt atccggcact
    27121 gtggcatttg ttgtcagaac cgtggttatg tgcgctgatt gcgttatcgc tgaacagtgc
    27181 ggcgtatacc acgcagctgt tttacggtgc aattcgtgcg atcccagaag gtcagtggca
    27241 gtcctgtagc gccctgggaa tgagcaaaaa agatacgctg gcgatcctgc tgccgtatgc
    27301 ctttaaacgc tcgctctctt cttattccaa cgaagtggtg ctggtgttca aaagtacctc
    27361 tctggcttac accattacgc tgatggaagt gatgggatac agccagttgt tgtacggacg
    27421 cacctacgat gtaatggtgt tcggtgcggc agggattatt tacctggtcg tcaacggcct
    27481 gctgacgctg atgatgcgtc tgatcgagcg caaagcactg gcattcgaac ggcggaatta
    27541 atgtagctgc gtacattcgc cggggggatt tgtaggtctg ataagacgcg ccagcgtcgc
    27601 atcagacatc agcacggtgc ctgtgccgga tgcggcgtgg acgccttatc cggcctacat
    27661 agtgcataaa ttcttataat gaagacggac aactaactaa gttatccgtc tttttttatt
    27721 tcatttaaat tatttaatca tgtttattgc atataaattc acttggtggc attgttatcc
    27781 catgccgcag acacggccaa aaatcataag attgacagac gggagttcca tcatgaaaaa
    27841 gttagttctt gccgctttac ttgcttcctt tactttcggt gcttctgccg cagagaaaat
    27901 caattttggc gtttcagcca cctatccacc ctttgaatct ataggtgcta ataatgagat
    27961 tgtcggcttt gatatcgatc tggcaaaagc cttgtgcaaa caaatgcagg cagaatgtac
    28021 ttttactaat cacgcgttcg acagcctgat cccgtcactg aaattcagaa aatatgacgc
    28081 cgtaatctcc ggtatggata tcaccccgga gcgtagcaag caggtttcgt ttaccacacc
    28141 ctactatgaa aactcagccg tcgtgattgc caaaaaagat acctacaaaa cgtttgccga
    28201 cctgaaaggc aaacgcattg ggatggaaaa cggtacgacg caccagaaat atattcagga
    28261 tcagcacccg gaagtgaaaa ctgtctccta tgacagttat cagaatgcct ttatcgatct
    28321 gaaaaatggt cgtattgatg gggtatttgg tgacaccgcg gtggtaaacg aatggctgaa
    28381 aaccaatccg caactgggcg ttgcgactga aaaagtgacc gatccgcaat acttcggcac
    28441 cggcctgggt atcgctgtac gtccggataa caaagctctg ctggaaaaac tgaataacgc
    28501 gctggcagca attaaagctg acggtacata tcagaaaatc aatgaccagt ggtttccgca
    28561 gtaattgaga ctgatgacaa aagtaagatt gcctgatgcg ctgcgcttat ccggcccaca
    28621 tcaactctgc aatgtattga tttagcatga ttttgtaggc cggataaggc gttcacgccg
    28681 catccggcat gaacaaagcg cacctgatca gcaatctgaa gccgcacctg tggctttttt
    28741 attgcttcac cagcagcgtc aacacttcat agtgcgcggt gtgcgggaac atatcaaaaa
    28801 gctgtacccg ttcgatgcgg taacctggca gttcgcggat atctttcgcc atggtttggg
    28861 cgttacagct ggagtagata ataaaacgcg gtgccatcgt tgaaagataa tcacacagcg
    28921 gtttaccaat gccgcggcgc ggcgggttaa ccagcaccag ctccggcacc tccccctgag
    28981 cagtggcaaa ctgagtggag tccagcgcct gaaattgtaa acgagttaag cccagttcag
    29041 cggctgactg ctttgcacag gcaatggcct ctggcgcaat ttcgatcccg gttaactgca
    29101 tgtcaggcgt cgcgcagtgt aaaccaaagc cccccacacc gcagaacaga tcccacatat
    29161 gtttaaccgg cagctgtcgt acccagtcgc gcgcggtggc gtacaactgg ctggcgaccg
    29221 ccggattagt ctggaagaaa ctttgcggac ggatccacag cggtacgtca ttaaaacgct
    29281 ccgccagtgc ctgttgttcg gtcaggtaga tctccgtctc cccttccata atcgccatat
    29341 gtaccggctg aatattgacg gtaataactt tcagctgcgg tagttgttcg tgtaaccacg
    29401 gcagcgcctt acgcagttgc gccagcttgg tatcagaacg cagtacaaag cgcagcatca
    29461 tgccgccatc actctggctt tcagtcagca gaatgtattt cagttcgcca cgcttacgcg
    29521 ccacgttgta gggcgttaac cccgctcggg cgataaacgg ttttagcgcc gcaaaaacgg
    29581 gcgcaaatga ggctggataa agcgggcagt cacaaaggtc ttccggcgtg ccatcgcgat
    29641 gcagcatacc gagcagtgtt ttttcaacgc taccactcac caccattttg gctttattac
    29701 gaaacccttg ttccgggcct gacaccggcg cgcaccattc ctcaaccgga aaatcggcga
    29761 gcagattttt aagatcggcg gttttagcgg agagttgctc tggaatcggc tgcgttatcc
    29821 actgacagga acgacagcga cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac
    29881 atcatcaggg gcgacgatta tacacattat tgcaactgga agaaccgccg actggtcgag
    29941 ggaacgaaca gcagcatcag gatgagcata tccggcagct tctgcagcat caggctatgg
    30001 aagatttcac gttttgattc accgggaatg ctgaacagct ccggataacc gtaccccagc
    30061 gaagccgccc acaagtaact tgcggcggtg atttgtgtca gcagatatag ccagcgcgcc
    30121 cagcgacgcc ctttcaccag tgaaaacgca caccagatct caatgaaaac cagcaccaga
    30181 ctgcttaaaa agaccagcgt taaactccag gtttgtacgc tgcgatgaat gaactcgcca
    30241 ataccgcgca cgcccagcgt attgaaaatc atcagcacgt cgaggccacg gatcataata
    30301 atggcgagcg ccgccacctg caccagcgca ggcacattca ggcgagcatg agatgaagat
    30361 gttttcttaa aaaatcccaa cgtttcgtct tccatgaaaa caatgccgcg acatgcgcag
    30421 cattatgtag ccaggttggc aaattttagt gtcttcagcc acgtcttgca cgttggatgt
    30481 cgcgtatccg ctgtttttct gcgcgcgcca tcagatacca ggcgataaat ccgacaattc
    30541 cgaccgcgcc gaggatcaat gttgccaggg cgttgatttc cggattcacc cccatccgca
    30601 cgctggaaaa gaccagcatc ggtaacgtgg tggctcccgg cccggaaaca aagctggcga
    30661 tcaccagatc atcaagcgac aaagtaaaag ccagtaacca gccagaaatg atcgcgggca
    30721 tgatcatcgg tagcgtaatg acgaaaaaca ctttcagcgg cgtcgcaccg agatccatcg
    30781 cggcttcttc tatcgagcga tccagttccc gcagacgcga cgaaataacg accgccacat
    30841 aagccgtaca gaaagtgaca tgcgccagcc agatggtaag cataccgcgg tccgcaggcc
    30901 agccaatggc atgagcaagc gcgacgaata ataacaacag cgacaagccc gtgatgacat
    30961 ctggcattac cagcggcgcg gtgatcataa aggcaaagcc atttgatccg cgaaacctgc
    31021 caaagcgcac cagcaccacc gccgcaatag tcccgaggat cgccgccgcc gttgccgcac
    31081 aggcagcaat tgtcaggctt gaaccaaccg cactcatcat cgcgtcatcg cgcaataact
    31141 caccatacca gcgcgttgac cagccggccc acaccgtcac cagcttcgag ctgttaaacg
    31201 aatagataac cagcatcagc attggcgcgt agagaaaggt aaagcccagc agcaaaatca
    31261 caatccgcca gggcgaacga actaccggta aattattcat ccgtgttctc ccacgctttt
    31321 ttgctggtgt ttgtggaacc acattatcgg cacaattagc agcagcaaca tgatgatcgc
    31381 taccgccgag gccaccggcc agtcgcggtt attgaaaaac tcttgccata gcacgcgccc
    31441 gatcatgatg ctgtccgggc caccgagcag ttccgggatc acaaactcgc ccacagccgg
    31501 gataaacacc agcatcgatc cggcaataat cccaccttta gtcagcggca cgatcacagt
    31561 aaagaacgtt ttcagcggtc gtgcaccgag atccagcgct gcttccacca gcgaataatc
    31621 aatacgaatc aacgcggtat aaatcggcag caccataaac ggcacgtagg cgtaaacaat
    31681 gccaatataa actgccagat tggtatgaag aatggtcagc ggttgatcga taacccccag
    31741 ccacagcaga aaattattca gcacaccgtt gttttttaat attcccatcc aggcataaac
    31801 gcggatcaga aacgaggtcc acgacggcag gatcaccagt agtaataaaa tattacgggt
    31861 cgaaggctta ctgtgcgcca ccgcccacgc cagcggatag ccgatcagta aacagcaaaa
    31921 tgtcgaaatc gcagccacct gtagcgactg gagataagca tcgaaataga gcggatcgtc
    31981 ggtcagttgc agaaaattac cgagattaag agtgatggac agttgcccgt cagcccactc
    32041 catcagttcg gtataaggtg gaatagcgcg cgccatctcc gccaggctta ttttaaagac
    32101 aatcagaaat ggcagcagaa acagcaagat caaccagata tatggcaacg caatgaccag
    32161 tttgcgccca tgcttcattt gcagctgtga caaccacagc ttaaatccgc ccggcggttt
    32221 cgactgggca gcaggttcaa gtgtattcat ctttgctcct taaaccgtca gcaccacact
    32281 gctgtccact tcccagcaca aacgcacttc gtcgccctag gtcggtaacc ctttacgatg
    32341 acgatgggcg ttttgtagct gggcgctaat catctgccca cttttcagac gaacgtgata
    32401 caccgaaaga tcgccgagat aggcaatgtg tatcacctcc cccaccgcga agttacaacc
    32461 attggcgggc ggctcttcgc aaagcatgat tttttccggg cgcagcgcca catgtaccgg
    32521 cacgttatcg accaccgagg catccgcgtc gactttcagt ggatgcacca gccccggcga
    32581 atcaagcacc aggccatctt cctgacgctc tttgagtacg ccttcaaaga cgtttaccga
    32641 gccaataaat tcggcgctat agcgggtagt cggatgctcg tagatctctt ccggttcgcc
    32701 aatctggaca aatttcccac gattcataat ggcgatgcgc cccgccatgg tcatcgcctc
    32761 ttcctgatcg tgggtgacca tcacacaagt cacaccgacg cgctccagaa tatccaccac
    32821 ttcaagctgc atccggtcac gcagcttttt atccagcgcg cccatcggct cgtcgagcag
    32881 taatagtttc gggcgcttgg caaggcttcg ggccagggcc acacgctgtc gctgaccacc
    32941 agaaagctga tgcggtttac gtttggcgaa ctcctgcatg tgcaccaggc cgagcatctc
    33001 attgacccga ctggaaattt ccgctttcgg tagtttgtcc tgtttcaggc caaaagcgat
    33061 gttctgttcc acggtcatat gcggaaacag cgcgtaagac tgaaacatca tattgatggg
    33121 gcgcaggtaa ggcggaacct gtgacaaatc gacgccatca agcattatct gtccggcaga
    33181 aggttgttcg aaacctgcca gcatacgcag cagcgtggac ttgccacagc cggatgcgcc
    33241 cagcagcgcg aagatttcac ctttgtagat ggtcaggctg acatcatcca ccgcatgttg
    33301 accatcgtag gatttggtga ggttgcggat ttctaatagc ggcgtcagca ccttacgggt
    33361 tttcgcctgc gggcgaggga tagcgtcatt cacggctgct ctccggcatt gaatgtgtgc
    33421 aagcgcacca tgatggtgcg aattgccggc gggtgtggtg cgcccgcccg gcatctaagg
    33481 ctgcggatta ttttccgctc ttcactttgg tccacgcgcg ggtgcgcaca cggtcgattt
    33541 tcgggtcctg cactttcaga gtgaacagct tcgcacgaac atccgcaggc ggataaatac
    33601 ccgggttctc acggacttcc gcactcacca gcggcgtggc tgctttattg gcgttgacat
    33661 agaacacatg gtcggaaata tgcgctacta catccgggcg cagcaggtaa ttcaggaact
    33721 gataggcttc gtctttgttt ttggcatccg caggcatggc gaatacatca aagaacgcca
    33781 tcgccccttc ttttggaatc gagaacgaga cattcactcc attcttcgct tccttcgcgc
    33841 ggtttgacgc ctgccagaca tcacctgccc agccgatagc gacgcaaata tcgccgtttg
    33901 ccaggtcgtt aatgtattga gatgaatgga aataacgaat gttcgggcgc agctttaaca
    33961 gcagatcggt tgccggtccg gtgtaatcat ccgctttagt gctgttggga tctttgccga
    34021 gataattcaa cacggtagca aaaacttctt ctggcgcatc caggaaagag acaccgcagc
    34081 ttttcagttt ttccagattt tcaggtttga ggatcaagtc ccagctatcg acgggcgcgt
    34141 tttcgcccag cacagcttta actttatcaa cgttatagcc aatcccggtc gtcgcccaca
    34201 tatagggcat agcaaattta ttgtcgggat cgtgtttggc gaccagcttc agcagctccg
    34261 ggtcgagatt cttccactcc ggcaatttgc ttttgtccag cggctggaaa actcccgcag
    34321 tcaactggcg ttcaagaaag ctggcagatg gaaccaccaa gtcaaagccg gtgctacccg
    34381 ccattaattt gccttccagt acttcgttag agtcgaaaac atcgtagacg actttaatac
    34441 cggtttcttt ttcaaaattg gccaccgtgt caggggcgat ataatcagac cagttataaa
    34501 tgtggagtgt tttttgttca gccgcgagcg tgccgacaga gacggccatc agagcacccg
    34561 caaccagacc cgatagccat tttttattta aggcggtcac atctctttcc ttctgaaagt
    34621 tcgttaacaa acgaacaaaa gttgtcacac tgaaagatat gcaataaacg tgcatatttt
    34681 tttatcggcg cggtgtaaga aaagagaact ctctcccggc cagcactgct cgctatatag
    34741 aagcatcgca agaataactt tagccagggt ttgagactat agctcggatt aaaaaacggt
    34801 atttaacaaa tttttatgcg tatttgctct atgtgataaa gccaaatcga cacaagagaa
    34861 gtgaaataat ctttaagaaa aggttaaaaa cagcagataa ttttgcagcg cgcagtcact
    34921 gcggcagcga ctgcgtgctc gaaaggaatt atcagtggag aaaatagttt tcggtaacgt
    34981 tgttttgccc ttcatcgtca gtcggtaaca gcagttgatg ggcattagct tcaagaatga
    35041 ccatcgaaat ctgctcttcg ctctgacgca cgaaccatgc aaactgctca taggttacgc
    35101 cctgcataac ggataaagac tggcaaacca ccagctttgg cagattatca tcctgcatat
    35161 caagaaatgc tttcacggtc agcgaactgg cattgatggc agataaatcc gccgccagcg
    35221 gcaatacggc tgaaggtcgg acttccgcca tggcagaaaa caggatcgtg ttatcgatca
    35281 gatcgatttt ggcatcaaag acaccgtcga aattctgcat atggggcaga tgcagagcct
    35341 gacagttatt acattcaaaa aaactcatcc ccaggccatc gagccattga cgcagcgtat
    35401 ccagaccagg aacgaccagc gatgtcataa ttttgtacaa cctctttcga taaaaagacc
    35461 ggcacagctt acgcaaaaag cgcaggcaaa accatgatca gtaatgtgat tgcgactaac
    35521 cacccgtttt caggcaatat tctgtcgtag cgtggcgttc gatccagcgg atcattttac
    35581 ccgcgatatc gataccggtg gttttttcta ttccttccag ccccggcgac gcattcactt
    35641 ccatcaccaa cggcccgcga ttagcacgca gaatatccac accagcaacg tccagcgcca
    35701 tcgttcgcgc ggcttttatc gcgatttcac gctcctgtgg tgtgatactg gcgacgcttg
    35761 ccgcgccgcc acgatgcaaa ttggaacgaa aatagccctc tttcgcccgc cgttcaatcg
    35821 cagcgaccac ttcatcacca acaaccagac agcggatatc gcacccttgc gcctctttga
    35881 tatattcctg caccagaata tgcgcgttca gaccgcggaa agcgtcaatc acgctttccg
    35941 ccgcctgacg agtctccgcc agcacgacgc caattccctg cgtgccttca accaacttga
    36001 ccaccagcgg cgcaccaccg accatgtcga ttaaatcgct ggtatcatcc ggcgaatgcg
    36061 caatgcccgt gacaggcagg tcgatgccct gacgcgccag cagttgcatg gaacgcaatt
    36121 tgtcacgcgc ccgggcaatg gcgaccgact cattgagcgg atagctcccc agcatctcga
    36181 actggcgcag tgccgccgtc ccataaaagg taatggcggt gccaatacgc gggatcactg
    36241 cgtcaaaatg gggtaacttg cggcctttgt agtgaataga agacgccgca ggatttatgt
    36301 tcatgtagca agaaagcgga tcaagaattt caaccaggtg accgcgctgt atagcggctt
    36361 cacgcagccg cttacacgaa tagagcgttc catcccggga caatatggca attttcaccc
    36421 tgcacctctc tgtcagacct ggtcaaaagc ccgcgtatca tacatcgacg tggcggtttt
    36481 agcgcgtcgc ccaaccctgt ttgtgcaaat aatccagaat aaatgggcgg ctttctttaa
    36541 tgattgttcg gcggatatga tcgctccagg tatcccagcg attattgctg ccacgggtga
    36601 ggtaatattc cgccagttgc tcgtcatact gcgccagtgc gtctttatcc agcggttgat
    36661 agctgttttc atgcaccaaa atggaggccg gtaaacgcgg cttgagatcc ggattatccg
    36721 caggccagcc aaggcacagc ccaaacagcg gcagaacatg ctgcggtaat ttcagcagtt
    36781 tcgtcaccgc ttcaatatta ttgcgcaggc cgccgatata taccccgccc aatcccagcc
    36841 gattccgctg cggttaatgc attctgcgcc atcattgccg tatcaacgac accgagcaac
    36901 agttgttccg ccaggccgag ctgagcatcc ggacagatct gtaaatggcg gttaaagtcg
    36961 gcacagaaca cccagaactc cgccgcttgc gctacgtgtt tttgcccgcc ggtcagcgtc
    37021 accagttctt cacgtaatgc tttgtcggta atgcgaataa tgctactaca ctgcaaaaaa
    37081 ctggaactgg acgtcgcacg ggcgctgtta ataatcgcct cacactgcgc ttcggaaatg
    37141 ggttcatcag tgaaatggcg aatggagcga tggccacaaa taagttcaat ggttggcgtc
    37201 attatctttt tctctttctg aacgtgaata ttgcggtgga cggttcatca gctgtggggc
    37261 aagacgtttt accacctgaa gaataaccac caccgcagcg ggaagcatga gcaaaacacc
    37321 gagaaaaatc atcagaatct gcacttctgg ccgagaaaat ggctcaggca gcgacaggga
    37381 gtcgcttacc gacagcagcg ccaccgccag tagcatcatt ccgataaatt ccagtatcaa
    37441 cacgccttta ggcaatttac cgatcgcgcg catacgcttc cctctgcaaa gtgagccttc
    37501 agtctaaaac ttttcactgt attgtgttta acagttatag cttttagcaa ttaatgcaac
    37561 aggttaaacc tactttcagc gaatacattt tagcgtgatc attacaggca taaatctatg
    37621 aggagagaaa taatgcaaac cgttattttt ggtcgttcgg gttgccctta ctgtgtgcgt
    37681 gcaaaagatc tggctgagaa attgagcaat gaacgcgatg attttcagta tcagtatgta
    37741 gatattcgtg cggaagggat cactaaagaa gatctacaac aaaaggcagg taaacccgta
    37801 gaaaccgtgc cgcagatttt tgtcgatcag caacatatcg gcggctatac cgattttgct
    37861 gcatgggtga aagaaaatct ggacgcctga tcgtctgaca agccctcgcg ttgagggctt
    37921 tactgatttt ttctgtgctg tggtttaaac aaactactga taaataagaa acacagtgcc
    37981 cccagcgcac accagaacac cgcgcttagt aaccatgcca gctcttgcca gaatgagcgc
    38041 gtcggtgaaa aaaacagccg cataatgagc attgaacagg gtgccgccag cattgcgcca
    38101 aatagaggtt tcaggacttc tctacgctgt gaaaaaaaac tggcaactgc tccaggaaga
    38161 atgaaaaaca gcaagccgat ttcaggatgc ccggcagccc gaaaagcgcc tttcatgtgc
    38221 gtcgccagaa aaaggcacac cacaatgaag aggacaaaac agcagattgc ccccgcccaa
    38281 cgttgtttat gtttcactcg ttcctcctga cactgcgtct atcgaacaca tttttcgcca
    38341 gtgtggcgtt cagtaagata aagccgcttc gcattccatg ctaatatagg ccaacgcaat
    38401 tcatatagcc gttgatacct aatgtgatta cactagtaaa atatattgtt actttactat
    38461 cgtttaggtg cgctgaatga atctgcaccc tgaattctgg taaaacacat tatcgtaaat
    38521 taccatttct ttcaacagct tactagtaaa caagaagtta gcctccgtga atataaacgt
    38581 cgccgaattg ttaaatggga attacattct gttattattt gtggtcctcg cgcttgggct
    38641 atgtctcgga aagttacgac ttggttcgat ccaactgggt aattccattg gcgttttagt
    38701 cgtatcgctg ttattaggcc aacaacattt cagcattaac accgatgcgc ttaatcttgg
    38761 ctttatgctg tttattttct gcgtcggggt cgaagccgga ccgaactttt tttccatttt
    38821 ttttcgcgat gggaaaaatt acctaatgtt agcactggtg atggttggca gtgcgctggt
    38881 gatcgcctta gggttaggta agctgtttgg ctgggatatt ggcctgacgg ccggtatgtt
    38941 agcaggctct atgacgtcga caccggttct ggtcggtgct ggcgatacac tgcatcattc
    39001 cggcatggaa agcaggcagc tctcactggc actggataat ctgagcctcg ggtatgcctt
    39061 aacctattta atcggtctgg tgagtttgat tgttggtgcg cgttacttgc cgaaattgca
    39121 gcatcaggac ttacagacca gcgcccagca aatcgcccgc gaacgtggcc tggacactga
    39181 tgccaaccgt aaggtttatt taccggtgat ccgcgcctat cgcgtcggcc cggagctggt
    39241 ggcctggacc gacggcaaaa atctgcgtga actgggtatt tatcgacaaa ccggctgcta
    39301 cattgaacgt attcgacgta acgggattct ggcaaatcca gacggtgatg ccgtgctaca
    39361 aatgggcgat gaaatagcgt tggtaggcta tcccgacgcc catgcccgac tcgatcccag
    39421 cttccgtaac ggtaaagaag ttttcgatcg tgaccttctc gacatgcgta tcgtcactga
    39481 agaagtggtc gttaaaaacc ataacgctgt aggtaaacgt ctcgcacaac tgaagttgac
    39541 cgatcacggt tgcttcctta accgcgtcat tcgtagccag attgagatgc cgatagatga
    39601 caacgtcgtg cttaacaaag gtgacgtttt acaagtcagc ggtgatgccc gtcgcgtaaa
    39661 aaccatcgcc gatcgcatcg gctttatctc gattcacagc caggtcactg acctgctggc
    39721 attttgcgcc ttctttgtta ttgggctgat gatcgggatg atcaccttcc agttcagcac
    39781 attcagtttc ggcatgggga acgctgccgg gttgttattc gccggaatta tgctgggctt
    39841 tatgcgtgct aaccacccga ccttcggtta cattccgcaa ggtgcattaa gcatggtgaa
    39901 agagttcggc ttgatggtgt ttatggcagg cgttggtctg agcgccggta gcggtattaa
    39961 taacggcctg ggcgcgattg gcggtcagat gttgattgcc ggattaattg tcagtcttgt
    40021 gcccgtggtt atctgtttct tgttcggtgc ttatgtattg cgaatgaacc gcgcactgtt
    40081 gttcggcgca atgatgggcg cacgcacctg cgcgccggca atggagatca tcagtgatac
    40141 agctcgcagt aacatccctg cgctgggcta tgcgggcacc tacgcaatca ccaacgtcct
    40201 gctgacgctg gcagggacaa tcatcgtcat ggtatggcca ggattaggat aaaactgaag
    40261 ttgccctgaa aatgaaattt ttttgcacaa ccgcagaact tttccgcagg gcatcagtct
    40321 taattagtgc cactgctttt ctttgatgtc cccattttgt ggagcccatc aaccccgcca
    40381 tttcggttca aggttgatgg gttttttgtt atctaaaatt tatctactac cctgcaaccc
    40441 tctcaaccat cctcaaaatc tcctcgcgcg atagcggctt acggtcggtg acaaaatgca
    40501 gcgtcatccc ctcaataaac gcatcaagcg cgcgggcggt tccgggttca aaccattgtt
    40561 cgagcgtttg ctgactgcgc tgcatccagt tttgcattac cgtttttaat agcggttttc
    40621 ggctagccag cgcgtagagc tggtacatca gctccatatt atccggcgtt gcaacctgtg
    40681 agctgtagat catatcggtg atagcctggc atgcgccttg agcatcacta acatcgctaa
    40741 aaaatgcctg atattgccgg gacataatct cagtaaaact gctgaacgct tccagcaaca
    40801 actcatcaat tcctgaaaaa tagtaggtca tcgatcccaa cggtaccccg gcaagggtag
    40861 caattttgcg gtgcgtaaca gcatgtattc cgtaaagttt caccgcctcc agcgtggcct
    40921 ggataatttt ttctcgccgt tgcggatcgt tagcgcgacg catgtcattt cctctgtctg
    40981 caattgtgta caaatgtaca caaccttgct aatgttgtct tccctcttct tattttgacg
    41041 tgatctatga ccgtaaattc ttcacgtaat gcattgaaac gccgaaccag ggcgctgttt
    41101 atgttcttct ttttgccagg cctgttaatg gcgtcctggg caacccgtac gcctgctatc
    41161 cgcgatattc tctctgtctc gatcgctgaa atgggcggtg ttctctttgg tctgtcgatc
    41221 ggttcgatga gcggtattct ctgctcggcg tggttagtga aacgctttgg gacacgtaat
    41281 gtcatcctgg tcacgatgtc ctgcgcattg atcgggatga tgatattaag tctggcactc
    41341 tggctgacat cgcccctgct ctttgccgtt ggtctcggcg tctttggggc aagttttggt
    41401 tctgcggaag tggcgataaa cgttgaaggt gccgccgttg agcgagaaat gaataaaacg
    41461 gttttgccga tgatgcacgg tttttatagc ctgggcacgc tggcaggcgc tggtgtcggg
    41521 atggcactga cggcctttgg cgttccggca acggtgcaca ttttattggc ggcgctggta
    41581 ggtatcgcgc ctatttatat cgccattcag gcaatccctg acggtacggg caaaaatgct
    41641 gccgatggca cccagcatgg cgaaaaaggc attccttttt atcgcgatat ccagttgctg
    41701 ttgattggtg ttgtagtgct ggcgatggcc tttgccgaag gttctgccaa cgactggtta
    41761 cccttattaa tggttgatgg tcacggtttt agccccactt ccggctcact gatttatgcc
    41821 ggttttaccc tggggatgac cgttggacgc ttcaccggcg gttggttcat cgaccgttac
    41881 agtcgcgttg ccgtggttcg ggccagtgca ctaatggggg cgttgggtat tgggctgatt
    41941 atttttgtcg atagcgcctg ggtcgcagga gtgtctgttg tactttgggg actgggtgcc
    42001 tcgctgggct tcccgctgac catttctgcc gccagcgata ccggccccga tgcaccaacc
    42061 cgcgtcagtg tggtagcaac gaccggttat ctggctttcc tcgtcgggcc gccgctgctg
    42121 ggctatctcg gcgaacatta tggattacgt agtgcaatgc tggttgtact ggcgctggtt
    42181 attctcgcgg ctattgtcgc gaaagccgtc gccaaacccg ataccaaaac gcagacggcg
    42241 atggagaata gttgatgagt attaaattaa ttgcggtaga catggatggt actttcttaa
    42301 gcgatcaaaa aacctataac cgtgagcggt ttatggctca gtatcagcaa atgaaagcac
    42361 aaggcattcg ctttgtggtc gccagcggga atcaatatta tcagttgatc tctttcttcc
    42421 ctgaaattgc gaatgaaatt gcctttgtgg ctgaaaacgg cggctgggta gtgagtgaag
    42481 gcaaagatgt ttttaatggc gagctgtcga aggatgcgtt tgctactgtc gtggaacatt
    42541 tgctgacgcg cccggaagtg gaaattattg cctgcggaaa aaatagcgcc tatacactca
    42601 aaaaatatga cgatgccatg aaaacggtgg cggaaatgta ttatcaccgt ctggaatacg
    42661 tcgataactt tgacaactta gaagatattt tctttaagtt tggcctgaat ctttccgatg
    42721 aactgattcc acaagtacaa aaagcattac atgaggccat cggcgatatt atggtgccag
    42781 tccacaccgg caacggcagc atcgatctga ttatccccgg cgtacataaa gccaatggcc
    42841 ttcgccaact gcagaaatta tggggaatag acgacagcga agtggtggtc tttggcgatg
    42901 gcggtaacga tattgagatg ctgcgtcagg caggctttag ttttgcaatg aaaaatgccg
    42961 gcagcgcggt cgtcgcagcg gcaaaatacc gggcaggctc caataaccgt gaaggcgtac
    43021 tggatgtgat cgataaagtt cttaaacacg aagcgccatt tgaccaataa aaacggcagc
    43081 acgcccggca acacaatgcc tgaatgaagg ctgcataatt cgcggaaatt aacccgctgc
    43141 attatttcag gcgaaatgat tatgaaaaat tgtctactgt tgggcgcgct tttaatgggc
    43201 tttactggtg tggcgatggc gcaaagtgtc accgtcgatg tgccgagtgg atacaaagtg
    43261 gttgtggttc ctgattcagt cagcgttcca caagcgtgtc agcgtcgcga ctgtgccgca
    43321 aaccgtctat gtggctcctg tgccagctcc ggcttaccgc ccgcatcctt atgttcgcca
    43381 tcttgccagc gtaggtgaag ggatggttat cgaacaccag attgacgacc atcaccatta
    43441 attcgagaat gcctgatcgc acaaatcaat caggcatttt tttacccttc gtgagaattt
    43501 cccatctgtt tatcttttaa aaagataacc atcagcgaca gccacaaaat tccgttgaca
    43561 aggttgaaga gattaaacag tccgttgccc ccgttcagcc aggcatgttt gctgatttca
    43621 ataccgacgg taaagatcag catttgcagc attcccatcg ccgcagaaac cgtaccttta
    43681 ctcatatcgc tggcaaacag ggttaatcgc accagtcccg cattcgccag accaataccg
    43741 aaagcataaa tacttaaccc ggcggtcatc cataaatacg cgtgcgatga gataaccgtt
    43801 gccgcagcag cgaccaacag accaatcata atcggccagc cgcccataat aatcagcgaa
    43861 cgtacggtgc ggcgcgaggt cagacgcgct aacagcaagt tacccgcaat taacgccccg
    43921 aaaataggca cttgcagcaa gccatattca tagctgctca actgctcccc ggtaatgatg
    43981 ataatcggcg actgggcgat ccacgccagc aatggcaggc taacgaatcc cagc
//
LOCUS       DADLTF010000014        43444 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.014, whole genome shotgun sequence.
ACCESSION   DADLTF010000014 DADLTF010000000
VERSION     DADLTF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.014"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..413
                     /locus_tag="JN749_000770"
     CDS             <1..413
                     /locus_tag="JN749_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7367277.1"
                     /translation="MYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKL
                     FWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFD
                     KYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            438..3037
                     /locus_tag="JN749_000771"
                     /pseudo
     CDS             438..3037
                     /locus_tag="JN749_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3028..4098
                     /locus_tag="JN749_000772"
     CDS             3028..4098
                     /locus_tag="JN749_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7367278.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESSDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQVIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            4110..4652
                     /locus_tag="JN749_000773"
     CDS             4110..4652
                     /locus_tag="JN749_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7367279.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGAGVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIQLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4660..5175
                     /locus_tag="JN749_000774"
     CDS             4660..5175
                     /locus_tag="JN749_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7367280.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTLDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5141..5878
                     /locus_tag="JN749_000775"
     CDS             5141..5878
                     /locus_tag="JN749_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HAY7367281.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5989..6999
                     /gene="pyrD"
                     /locus_tag="JN749_000776"
     CDS             5989..6999
                     /gene="pyrD"
                     /locus_tag="JN749_000776"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HAY7367282.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7173..7715
                     /gene="zapC"
                     /locus_tag="JN749_000777"
     CDS             7173..7715
                     /gene="zapC"
                     /locus_tag="JN749_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HAY7367283.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JN749_000778"
     CDS             complement(7712..8821)
                     /gene="ycbX"
                     /locus_tag="JN749_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HAY7367284.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9065..11173
                     /gene="rlmKL"
                     /locus_tag="JN749_000779"
     CDS             9065..11173
                     /gene="rlmKL"
                     /locus_tag="JN749_000779"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HAY7367285.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11185..13092
                     /locus_tag="JN749_000780"
                     /pseudo
     CDS             11185..13092
                     /locus_tag="JN749_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13222..14475
                     /gene="pqiA"
                     /locus_tag="JN749_000781"
     CDS             13222..14475
                     /gene="pqiA"
                     /locus_tag="JN749_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HAY7367286.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAVVIFLASIMVPTLKMSAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14480..16120
                     /gene="pqiB"
                     /locus_tag="JN749_000782"
     CDS             14480..16120
                     /gene="pqiB"
                     /locus_tag="JN749_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HAY7367287.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16117..16680
                     /gene="pqiC"
                     /locus_tag="JN749_000783"
     CDS             16117..16680
                     /gene="pqiC"
                     /locus_tag="JN749_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HAY7367288.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16936..17103
                     /gene="rmf"
                     /locus_tag="JN749_000784"
     CDS             16936..17103
                     /gene="rmf"
                     /locus_tag="JN749_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HAY7367289.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JN749_000785"
     CDS             complement(17173..17691)
                     /gene="fabA"
                     /locus_tag="JN749_000785"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HAY7367290.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKIT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17760..19520)
                     /locus_tag="JN749_000786"
     CDS             complement(17760..19520)
                     /locus_tag="JN749_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HAY7367291.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19705..20157
                     /gene="matP"
                     /locus_tag="JN749_000787"
     CDS             19705..20157
                     /gene="matP"
                     /locus_tag="JN749_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HAY7367292.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JN749_000788"
     CDS             complement(20233..21279)
                     /gene="ompA"
                     /locus_tag="JN749_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HAY7367293.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JN749_000789"
     CDS             complement(21635..22144)
                     /gene="sulA"
                     /locus_tag="JN749_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HAY7367294.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22363..22992
                     /gene="sxy"
                     /locus_tag="JN749_000790"
     CDS             22363..22992
                     /gene="sxy"
                     /locus_tag="JN749_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HAY7367295.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JN749_000791"
     CDS             complement(22955..25117)
                     /gene="yccS"
                     /locus_tag="JN749_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HAY7367296.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25127..25573)
                     /locus_tag="JN749_000792"
     CDS             complement(25127..25573)
                     /locus_tag="JN749_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAY7367297.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25696..27750
                     /gene="helD"
                     /locus_tag="JN749_000793"
     CDS             25696..27750
                     /gene="helD"
                     /locus_tag="JN749_000793"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HAY7367298.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSDVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JN749_000794"
     CDS             complement(27782..28240)
                     /gene="mgsA"
                     /locus_tag="JN749_000794"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAY7367299.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28336..28998)
                     /locus_tag="JN749_000795"
     CDS             complement(28336..28998)
                     /locus_tag="JN749_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HAY7367300.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29171..29584
                     /locus_tag="JN749_000796"
     CDS             29171..29584
                     /locus_tag="JN749_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAY7367301.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JN749_000797"
     CDS             complement(29629..29946)
                     /gene="hspQ"
                     /locus_tag="JN749_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HAY7367302.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JN749_000798"
     CDS             complement(30004..31194)
                     /gene="rlmI"
                     /locus_tag="JN749_000798"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HAY7367303.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31289..31567
                     /gene="yccX"
                     /locus_tag="JN749_000799"
     CDS             31289..31567
                     /gene="yccX"
                     /locus_tag="JN749_000799"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAY7367304.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JN749_000800"
     CDS             complement(31564..31893)
                     /gene="tusE"
                     /locus_tag="JN749_000800"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HAY7367305.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JN749_000801"
     CDS             complement(31984..32643)
                     /gene="yccA"
                     /locus_tag="JN749_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HAY7367306.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32850..32937)
                     /locus_tag="JN749_000802"
     tRNA            complement(32850..32937)
                     /locus_tag="JN749_000802"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32901..32903),aa:Ser,seq:tga)
     gene            33363..34481
                     /gene="hyaA"
                     /locus_tag="JN749_000803"
     CDS             33363..34481
                     /gene="hyaA"
                     /locus_tag="JN749_000803"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HAY7367307.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLTKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34478..36236
                     /gene="hyaB"
                     /locus_tag="JN749_000804"
                     /pseudo
     CDS             34478..36236
                     /gene="hyaB"
                     /locus_tag="JN749_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36255..36962
                     /gene="hyaC"
                     /locus_tag="JN749_000805"
     CDS             36255..36962
                     /gene="hyaC"
                     /locus_tag="JN749_000805"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HAY7367308.1"
                     /translation="MQQKSDNIVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36959..37546
                     /locus_tag="JN749_000806"
     CDS             36959..37546
                     /locus_tag="JN749_000806"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HAY7367309.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37543..37941
                     /gene="hyaE"
                     /locus_tag="JN749_000807"
     CDS             37543..37941
                     /gene="hyaE"
                     /locus_tag="JN749_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HAY7367310.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37938..38795
                     /locus_tag="JN749_000808"
     CDS             37938..38795
                     /locus_tag="JN749_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HAY7367311.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38929..40473
                     /gene="appC"
                     /locus_tag="JN749_000809"
     CDS             38929..40473
                     /gene="appC"
                     /locus_tag="JN749_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HAY7367312.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40485..41621
                     /gene="appB"
                     /locus_tag="JN749_000810"
     CDS             40485..41621
                     /gene="appB"
                     /locus_tag="JN749_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7367313.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41633..41725
                     /gene="cbdX"
                     /locus_tag="JN749_000811"
     CDS             41633..41725
                     /gene="cbdX"
                     /locus_tag="JN749_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HAY7367314.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41805..43103
                     /locus_tag="JN749_000812"
     CDS             41805..43103
                     /locus_tag="JN749_000812"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HAY7367315.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43218..>43444)
                     /locus_tag="JN749_000813"
     CDS             complement(43218..>43444)
                     /locus_tag="JN749_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367316.1"
                     /translation="AVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNIK
                     GAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10808 a        10584 c        11562 g        10490 t
ORIGIN      
        1 ttatgtacat tggtgcgccg ttggcgaaag acagagaaac ccttttcttc actagcgtac
       61 gggcagtccc ttcaacaacg aagcgggaag agggaaatac cctgaagatt gccacacaaa
      121 gcgtcatcaa acttttctgg cgaccaaaag gtttagcgta tcccttaggc gaggctccgg
      181 cgaaactgcg ttgcacttcg tcagctgaca tggttacggt cagtaaccca acaccttatt
      241 tcattaccct gacagacctg aaaataggtg gaaaagtagt taaaaatcaa atgatttccc
      301 cctttgataa ataccaattc tctctgccaa agggggccaa aaatagcagc gtaacgtatc
      361 gaaccatcaa tgactacggg gcggaaacgc cgcaactcaa ctgtaaatcg taagccgtct
      421 tcagttaaga gagcgagatg tatagaactc atcgacaaca cagcctgtta agctctggtg
      481 gagtgccatc gtttattggt gggctggtgg tgtttgtgtc ggcagcgtcc aatgcacaag
      541 ctgaaacctg gttcgatcct gcttttttca aagatgatcc ctcaatggtg gccgatttgt
      601 ctcgtttcga aaaaggacaa aaaataacgc caggggttta tcgtgtcgat attgttctga
      661 atcagacaat tgtagatacg cgcaacgtca attttgttga gataacgcca gagaagggga
      721 ttgccgcctg tttgacgact gaaagcctgg atgcaatggg tgttaatact gatgcgtttc
      781 cggcttttaa acaactggac aaacaagcgt gtgtgccatt ggcggagatt attccggatg
      841 ccagcgtaac gtttaatgtg aataaactcc gtctggaaat ttcagtaccg caaatcgcca
      901 tcaaaagtaa tgcccgtggt tatgtccccc ctgaacgttg ggatgaaggg atcaacgcgc
      961 tattactggg atattcgttt agcggggcta acagtattca tagcagcgca gacagtgatt
     1021 ctggcgacag ctattttctg aatttaaaca gtggcgttaa tttaggccca tggagattgc
     1081 gcaacaattc aacatggagt cgcagtagtg gccaaaccgc agaatggaag aatctcagca
     1141 gctatttgca gcgggcggtt attccactaa aaggcgaact gaccgtaggt gatgattata
     1201 ctgcaggcga ttttttcgat agtgtcagct ttcgtggtgt gcagctggcg tcagatgaca
     1261 acatgctgcc agacagcctg aaagggtttg cgcctgtggt gcgtggtatc gccaaaagca
     1321 atgcccagat aacgattaag caaaatggtt acaccattta ccaaacttat gtatcgcctg
     1381 gtgcttttga aattagtgat ctctattcca cgtcgtcgag cggtgatttg ttagttgaaa
     1441 tcaaagaagc ggacggcagc gtcaatagct acagcgtacc gttttccagc gtgccattac
     1501 tccagcgtca ggggcgaatc aaatacgcgg tgacactggc gaaatacaga accaatagta
     1561 atgaacagca ggagagcaaa tttgcccagg ccacgttgca gtggggcgga ccgtggggaa
     1621 cgacatggta tggtggtgga caatatgctg aatattaccg tgccgccatg tttggtctgg
     1681 gatttaacct tggtgatttc ggagcaattt cgttcgatgc gacccaggcg aagagtacgc
     1741 tggcagagca aagcgaacat aaaggtcagt catatcgttt tctgtatgcc aaaacgctca
     1801 accaattggg cactaatttt caattgatgg gctatcgcta ttcgacgtcg ggtttctaca
     1861 ccctttccga caccatgtat aaacatatgg atggctacga atttaatgac ggtgatgatg
     1921 aagatacgcc gatgtggtcg cgttattaca atttgtttta caccaaacgt ggcaaactgc
     1981 aggtcaatat ctcccagcaa ttaggcgagt acggttcgtt ttatttaagt ggtagccagc
     2041 aaacttactg gcataccgat caacaggatc ggctattaca gtttggctac aacacgcaaa
     2101 ttaaagatct ctcgctgggg gtttcctgga actacagtaa gtcccgtggt caacctgatg
     2161 ctgatcaggt gtttgcacta aatttttccc tgccgctcaa tctgttgctc cccagaagta
     2221 atgatagcta taccaggaaa aaaattacgc ctggatgacc tctaacacca gtatcgataa
     2281 cgaagggcac attacacaaa acctgggttt aacggagaca ctactcgatg acggtaacct
     2341 gagctacagc gtgcaacagg gatataacag cgaggggaaa acggctaatg gtagcgccag
     2401 catggactac aaaggggcgt ttgcagatgc ccgagtgggc tacaactaca gcgataacgg
     2461 cagtcaacaa caactgaact acgctctttc aggcagttta gttgcccatt cacagggcat
     2521 taccctgggg caatcgctgg gggaaactaa cgtgctgatt gtagcaccag gcgcagagaa
     2581 tactcgtgtg gcgaacagca ccgggctgaa aactgactgg cgcggatata ccgttgtccc
     2641 ctatgccact tcttatcggg aaaatcgaat cgcacttgat gcggcatcgt taaaacgtaa
     2701 cgtggatctt gaaaatgcag tagtcaacgt ggttcccacc aaaggggcgt tggttctggc
     2761 ggagttcaat gcccatgcgg gagcaagggt attaatgaaa acatcaaagc agggtatacc
     2821 gctgcgtttt ggcgcgatag cgacgctgga cggcatacag actaatagcg gcattattga
     2881 tgatgatggc tcgctctata tgtccggttt gcccgctcag ggagcgataa ctgtacgctg
     2941 gggcgaagct cccgatcaaa tttgtcatat cagttaccag cttaccgaac aacaaattaa
     3001 ctctgcgatt acgcgaatgg atgccatatg cagataatct ttggagaaaa atgcgtgtta
     3061 ttactacgac tattttttgc cgccgtctta atgctatggt gcgctcaaac tgctgcttat
     3121 atcgggcagt gtcataccac tcaggggaat ccgtatattg gcgtcaattt tggcgttaaa
     3181 actctggatg aagaagaaaa tacggctgga gtagttaaag acaaatttta tcagtggaac
     3241 gaatcgagtg attattatgt ttcctgtgat tgcgataaag acaatgtcag aagtggccga
     3301 tgggcattcg ccgcggattc accgttagtc tatttaggcg acaactggta caaaattaat
     3361 gactatcttg ccgccaaagt tttattgcag gttaaaggca gttctcctac tgcggttcct
     3421 ttcgaaaacg tgggcacagg ggcagataca cgatggcata tttgcgatcc cggcggtcaa
     3481 cgtttaggtg gccagggggc tagcggtaat agcggtagct tttccctgaa aatattgcag
     3541 ccgttcgttg gttcggtcgt cattcctcct atggcgctgg cgcgattatt tgaatgctac
     3601 aacatacccg caggtgattc ctgcacgact actggtacac cggttttagt gtattacctg
     3661 tctggtacga tcaattcact tggctcatgt tccgtcaatg ccggagaaac aatcgaggtt
     3721 gatctgggcg acgtatttgc ggctaacttt cgtgttgtag ggcataagcc tcttggggcc
     3781 agaacggcag aacttgcaat tccggtcagg tgtaacacgg gaaacgcagg gttagttaac
     3841 gtcaatctga gtctgacggc aacctcagac cccagctatc cccaggtgat taagacgtca
     3901 cgtcctggcg tgggcgtggt ggtgaccgat agccagaaca acattatttc ccctgctggt
     3961 gggacactac cgctctctat tcctgatgat gcagacagta tcgcgcgaat gaatgtctat
     4021 ccagtcagca cgacaggcgt accaccagaa accgggcgat ttgaagccac ggcaacggtg
     4081 agaataaatt ttgattaacg cgtgaacgta tgaagaaaaa aacgatatat cagtgcgtta
     4141 ttttgttctt tagccttctt aacatccatg tcgggatggc tgggcctgaa caagtcagta
     4201 tgcatattta tgggaatgtg gtcgatcagg gctgtgatgt cgccaccaaa agtgcattac
     4261 aaaatattca tattggtgat tttaatatca gtgattttca ggccgcgaat accgtaagca
     4321 ctgctgctga tttgaatatt gatatcaccg gttgtgccgc tggtattact ggcgcgggcg
     4381 tcctttttag cggcgaggct gacacccttg cgccgacact gctcaaacta actgacacag
     4441 gcggaagcgg tggtatggca acggggattg ccgtgcaaat tcttgatgcg caaagtcagc
     4501 aagaaatcca gctcaatcag gtccagcctc ttacgccctt aaaagccggg gataacacac
     4561 tcaaatatca acttcgttat aagtccacaa aggcgggagc aacgggcggt aatgcgacgg
     4621 cggttctcta ttttgatctg gtttaccagt gaaggagtga tgttgaaacg gataatctgg
     4681 attctgttct tactgggatt aacggggggc tgtgagctat ttgcccatga tggcacggtc
     4741 aacattagcg gatcgtttcg ccgtaataca tgcgtgctgg cacaggatag caagcaaatc
     4801 aatgtgcagt taggcgatgt ctcgctgaca cgtttttttc atgggaatta tggcccggag
     4861 aaatctttca tcattaattt gcaggattgc ggaacagatg tcagcacggt tgatgtcacc
     4921 ttttcaggaa ccctcgacgg agtgcagagc gaaatgctgt ccattgaaag tggtacggat
     4981 gcagccagtg ggctggcaat tgcgattctg gatgatgcga agatattgat tccgcttaac
     5041 caggcgagta aagactacag cctacatagc ggtaaggtgc cattaacttt ttatgcgcaa
     5101 ttgcgacctg tcaatagcga tgtgcagtcg ggtaaagtga atgccagcgc gacatttgta
     5161 cttcattatg actaatacgt ggaacagatt agcgctcttg attttcgccg ttttatcgct
     5221 gctggtggcg ggtgaattgc aggcaggagt ggtggtcggc ggaacgcgat ttatctttcc
     5281 ggcagacaga gaatcgatat ctattttact gactaatacc agtcaggaat cttggcttat
     5341 taacagcaaa atcaatcgcc caacgcgttg ggccgggggg gaagcgtcga cagtgccagc
     5401 actattactg gccgctccgc cacttattct cctgaagccc ggtactacag gcacgttgcg
     5461 cttgctgaga acggaaagcg acatcttgcc tgtggatcgc gaaacgctat ttgagttaag
     5521 cattgccagc gtgccatccg gcaaagttga aaatcagagc gtaaaagtgg cgatgcgctc
     5581 ggtatttaaa ctgttctggc gacacgaagg tttgccgggc gacccgctgg aagcttatca
     5641 acaattacgc tggacacgga attcgcaggg tgtacaactc actaacccaa cgccttacta
     5701 cattaacctg attcaggtga gcgtaaatgg taaagcgtta agtaatgcgg gagtcgtgcc
     5761 gcctaaaagc cagcgtcaga caaactggtg tcaggtaata gcaccgtgtc atgtcgcctg
     5821 gcggacgata aatgattacg gcgggttgag tgcaaagaag gagcaaaatc tgccctgaaa
     5881 caggttctga aaacgtttgc gttttttttg ccgcaggtca attccctttt ggtccgaact
     5941 cgcacataat acgcccccgg tttgcacacc gggaatccag gagagttcat gtactacccc
     6001 ttcgttcgta aagccctttt ccagctcgat ccagagcgcg ctcatgagtt tacttttcag
     6061 caattacgcc gcattaccgg aacaccgttt gaagcactgg tgcggcagaa agtgcctgcg
     6121 aaacctgtta actgcatggg cctgacgttt aaaaatccgc ttggtctggc agccggtctt
     6181 gataaagacg gggagtgcat tgatgcgtta ggcgcgatgg gatttggatc gatcgagatc
     6241 ggtaccgtca cgccacgtcc acagcctggt aatgacaagc cgcgtctctt tcgtctggta
     6301 gatgccgaag gtttgatcaa ccgtatgggc tttaataatc ttggcgttga taacctcgta
     6361 gagaacgtaa aaaaggccca ttatgacggc gtcttgggta ttaatatcgg caaaaataaa
     6421 gatacgccag tggagcaggg caaagatgac tatctgattt gtatggaaaa aatctatgcc
     6481 tatgcgggat atatcgccat caatatttca tcgccgaata ccccaggatt acgcacgctg
     6541 caatatggtg aagcgctgga tgatctctta accgcgatta aaaataagca aaatgatttg
     6601 caagcgatgc accataaata tgtgccgatc gcagtgaaga tcgcgccgga tctttctgaa
     6661 gacgaattga tccaggttgc cgatagttta gttcgccata atattgatgg cgttattgca
     6721 accaatacca ctctcgatcg ttctctggtt cagggaatga aaaattgcga tcaaaccggt
     6781 ggcttaagtg gtcgtccgct tcagttaaaa agcacagaaa ttattcgccg cttgtcacta
     6841 gaattaaacg gtcgcttacc gatcatcggt gttggcggca tcgactcggt tatcgctgcg
     6901 cgtgaaaaga ttgctgcggg tgcctcactg gtgcaaattt attctggttt tatttttaaa
     6961 ggtccgccgc tgattaaaga aatcgttacc catatctaat tatttcttcg aattcctctt
     7021 tataagataa tcagggcttt attttcagcc ctggttgttt tatattcatc gctgttgctt
     7081 atttagacat tttgtacttt tattgttgag gttattaagc gaagcaacaa tggattgtgt
     7141 tgctgcggtt ttatagggta ggggagaggc agatgcgaat taaaccagac gataactggc
     7201 gttggtatta cgatgaagag cacgatcgaa tgatgctcga tttagccaat ggtatgctat
     7261 ttcgctcacg ttttgcgcgc aaaatgttga ctccagatgc cttttctccc gcaggctttt
     7321 gcgttgatga tgccgcgctc tatttctctt ttgaagaaaa gtgccgcgat tttaatttat
     7381 ctaaagacca aaaagccgaa ctggtgctga atgcactggt agcaattcgt tatctcaaac
     7441 cgcaaatgcc gaaaagctgg cattttgtct cccatggtga aatgtgggtg cccatgccgg
     7501 gcgatgcggc ctgcgtttgg ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg
     7561 aatctggcga aaatgccgcg ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc
     7621 gcgcgatgca gttgggcgac gccattaaaa tcatgaacga caggctgaaa ccgcaggtga
     7681 atgttgacag cttcagcctc gaacaggcag tctaacgcgt cagcttaagg gcagtcttcg
     7741 gtacacagct acagcaaaga atggtgccat catcgcccat tgctgatttt ttcagcggcg
     7801 tgacttcgcc ttctaaaagc tgaacacggc aacttccaca aatgcccgcg cggcaagaat
     7861 aagggatacg aattccctga ttttctaatt gctccagcaa cacttgttgg ttatttccac
     7921 gaaatgcctg tccctgccag tcaatatcta catttgcgtc cggttgttgc gtgatgttgg
     7981 cggtatcatc agcggcagct gcgccgtaaa tttttgccgg agccgttgcc agaatttcca
     8041 cctcatcgcc aacgcgaatc acgccgctat tacgggcaat taaattctga ccaaaatcga
     8101 catcgccgtt atcctgggca gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg
     8161 gatgtttttg ccctttttct gggctgacgg tggtgaaaat acagcggcta caaggtttaa
     8221 ccacatcaaa caccacatca ccaatgcgaa tcactttcca gctatcttct tcccaggctg
     8281 acgcgccgga aaccaccaga ttggggcgga attgctccat ttttacactg gccggacaac
     8341 gttgttggag atcacgtaac gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa
     8401 atgacagagg tacagtgttg tggcgtttca cgcgccgggt catttgtggc cccacccagc
     8461 gtaattgcac ttcgcgggag aaaaatccac ttagccattt gttgatcgcg tctggcgcaa
     8521 ttcgcgcggt aaaatgtgtg ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag
     8581 caaaacgaac atatgcgcta ctgccatctg gtgcggtgag atgcaagcca tcatgcacag
     8641 gtgaaggagt aaaccgtacc atctggggaa actggcgagc ggtaataaac gtaccgtcag
     8701 gttccgtgat cataaagatg cgatcgaagg ccagaccact gacatcagcc agagcatgtg
     8761 taagaccaat gccgcgcatc gatttaacag gatgaataaa aagccgggtt aatgtcgcca
     8821 cggtgcggtc ctcaaatgaa aataagccct caactttatg acatgacgcg cttattagct
     8881 ataatgcgca acaattttct tagcgcctgt cccgttaggc tattttattt gtcagtttgg
     8941 ccctgggttg tgctcgaaat cctcacgtac ttgtgtacgc tccggtttct ccgcgctctc
     9001 catgtccaga ctgactgcaa caattacgcc tactgcggta ggctcttaga gtaaaagtga
     9061 cgatatgaat tctctgtttg ccagtacggc ccgtgggctg gaagagctgt taaaaactga
     9121 actggaaaat ctgggggccg ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg
     9181 cgacacacgg cttgtttacc agagcctgat gtggagccgc ctggcctcgc gtattatgtt
     9241 gccgctgggc gagtgtaagg tttacagcga tttagacctc tatctcggtg ttcaggcgat
     9301 caactggaca gagatgttta atcctggcgc gaccttcgct gtccacttca gtggtttgaa
     9361 tgacaccatc cgcaacagtc agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc
     9421 tttcacgcgg aaaaatctgc cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt
     9481 taacgtctgg ctgcataaag aaaccgccag tatcgctctt gatctgagtg gtgatggttt
     9541 acatctgcgt ggctatcgcg atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc
     9601 cgctattgtg atgcgatccg gctggcagtc aggaacaccg ctgctcgatc cgatgtgtgg
     9661 ttccggtacg ttgctgattg aagcggcgat gctggcgacc gatcgcgcac caggcttgca
     9721 ccgtgggcgt tggggcttta gcggctgggc gcagcatgat gaagctatct ggcaggaagt
     9781 gaaagcggaa gcgcaaactc gcgcccgtaa aggcctggct gagtatagct ctcattttta
     9841 cggttcggac agcgacgcac gggtgattca acgtgcgcgc actaacgccc gtcttgcggg
     9901 gattggtgaa ctgatcacct ttgaggtgaa agatgtcgcg caactgacca atccgctgcc
     9961 gaaagggccg tacggtacag tgttgagcaa cccgccatac ggtgaacgtc tggacagcga
    10021 accggcgctg attgcgctgc atagtctgct gggccgaatc atgaaaaacc agttcggtgg
    10081 ctggaatctc tctttgttta gtgcctcgcc ggatctgcta agctgcctgc agctgcgtgc
    10141 agacaaacag tacaaggcga aaaacggccc gctggactgc gtacagaaaa attaccacgt
    10201 tgccgaaagc acaccagaca gtaaaccggc aatggtagcg gaagattacg ccaaccgcct
    10261 gcgtaagaac ctcaaaaaat tcgagaagtg ggcacgccag gaagggattg aatgttaccg
    10321 cctgtatgac gccgatctgc cggaatataa cgttgccgtt gaccgttatg ccgactgggt
    10381 ggtggtgcag gagtatgcgc cgccaaaaac tattgatgcg cacaaagcgc gtcagcgtct
    10441 gttcgatatt atcgctgcaa ccatttcggt actggggatt gcgccaaaca aactggtgct
    10501 gaaaacccgt gaacgccaga agggcaaaaa ccagtaccag aaactgggcg agaagggcga
    10561 gtttcttgaa gttaccgaat ataacgctca cttgtgggtg aacctgacgg attatctcga
    10621 tactggtctg ttcctcgatc accgtatcgc ccgccgtatg ctcggtcaga tgagcaaagg
    10681 caaagatttc ctcaacctgt tttcttatac cggcagcgcc accgtgcatg cgggattagg
    10741 cggtgcacgc agcaccacca ccgtggatat gtcgcgtact tatctggaat gggcagaacg
    10801 caacctgcgt ctgaatggcc tgactaggcg tgcgcatcgc ctgattcagg ccgattgcct
    10861 ggcgtggctg cgtgaggcaa atgaacagtt cgatctgatc tttatcgatc cgccaacctt
    10921 ctctaactca aaacgaatgg aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat
    10981 gaaagatttg aaacgtctgc tgcgtgcagg tgggacgatc atgttctcga acaacaaacg
    11041 tggcttccgt atggatctcg acggcctggc aaaactggga ctgaaagcac aagaaattac
    11101 gcaaaaaacg ctctcccagg atttcgcccg taaccgccag atccacaact gctggctgat
    11161 taccgcagcc tgaaaggaat agtaatgtca ttaatcagta tgcatggcgc atggctgtcg
    11221 ttcagcgacg cgccgcttct cgataacgca gaactgcata tcgaagataa cgaacgtgtt
    11281 tgtctggtgg gccgcaacgg cgcaggaaaa tcgacgttaa tgaaaatcct caaccgtgaa
    11341 caagggctgg atgacggtcg cattatttat gagcaagatt tgattgtagc tcgtctgcaa
    11401 caggatccgc cgcgtaacgt tgagggtagc gtttatgatt tcgttgccga aggcattgaa
    11461 gaacaagcgg aatatctgaa acgctatcac gatatttcgt gcctggtgat gaacgacccg
    11521 agcgagaaaa atctcaacga actggcgaag gttcaggaac agctggatca ccacaacctg
    11581 tggcagctgg aaaaccgcat caacgaagtg ctggcgcaac tggggttaga tcctaacgtt
    11641 gcgctgtcgt cgctttccgg cggctggttg cgtaaagcgg cattaggacg cgcgctggtg
    11701 agcaatccgc gcgtattgct gctggacgaa ccgactaacc atctggatat cgaaaccatc
    11761 gactggctgg aggggttcct taaaaccttc aatgggacga ttattttcat ctcccacgac
    11821 cgttcgttta tccgcaatat ggcgacgcgc attgttgatc tcgatcgcgg caagctggtg
    11881 acctatccag ggaattacga ccagtacctg caggaaaaag aagaagccct gcgcgtggaa
    11941 gaactacaaa atgccgagtt cgatctcaaa ctggcgcagg aagaagtgtg gattcgctag
    12001 gggatcaaag cacgccgtac ccgtaatgaa ggccgcgtac gcgccctgaa agcgatgcgt
    12061 cgcgaacgtg gtgaacgtcg cgaagtgatg ggtaccgcaa agatgcaggt ggaagaggcc
    12121 agccgctccg gtaagatcgt tttcgaaatg gaagacgttt gctaccaggt taacggtaag
    12181 caactggtga aagatttttc tgcccaggtt ctacgtggcg acaaaattgc cctgattggt
    12241 ccgaatgggt gcggcaaaac cacgctgctg aaactgatgc tcggtcagct tcaagcgggc
    12301 agcgggcgta ttcacgttgg caccaaactg gaagtggctt atttcgatca gcaccgcgcg
    12361 gaactggatc ccgataaaac ggtgatggat aaccttgccg aaggtaagca agaggtgatg
    12421 gttaatggca agccacgcca cgtattgggc tatttgcagg actttctgtt ccatccgaaa
    12481 cgggcgatga cgccagtacg tgcgctttct ggcggtgagc ggaaccgctt gctgctggcg
    12541 cgtttgttcc tcaaaccaag caacttattg attcttgacg aaccgaccaa cgatcttgat
    12601 gtcgaaacgc tggaactgct ggaagaactg atcgacagct atcagggcac ggtattgctg
    12661 gttagccacg atcgtcagtt tgtcgataac accgttacag aatgctggat cttcgaaggc
    12721 ggcggtaaaa ttggtcgtta tgttggcggt tatcatgatg cccgtggtca gcaagaacaa
    12781 tatgtggcac tcaaacagcc tgcggtgaaa aaaagcgaag aagccgccgc gccaaaagca
    12841 gaaactgtaa aacgcagcag tagcaaacta agctataaat tgcagcgcga actggagcag
    12901 ctaccgcaat tgctcgaaga tctggaggcg aagctggaag ccctacagac gcaagtggcg
    12961 gatgcttcct tcttcagtca gccgcatgag cagacgcaaa aagtgcttgc tgatatggct
    13021 gctgcagagc aggagctgga gcaagccttt gaacgctggg agtatcttga agcgttaaaa
    13081 aatggtggct gatcgcaaaa tagtcgattt aaacagagac agctgacaac ggtaaaattg
    13141 cctgatgaac tgggtttatc aggcatgaaa accaacactt tgttagtcat ctgtaaaata
    13201 gcgcatcatt aaggagtacc aatgtgcgaa catcatcatg ccgcgaagca catcctgtgc
    13261 tcgcagtgtg acatgctggt ggcgttaccg cgccttgagc atggtcagaa agcggcatgt
    13321 ccccggtgtg gcacaacgtt aaccgtggcg tgggatgccc ctcggcagcg tcccaccgcc
    13381 tatgcgttgg ctgcactgtt catgctgttg ctgtccaact tgtttccttt tgtgaatatg
    13441 aacgttgcag gagttaccag tgaaattaca ttactggaaa ttcccggcgt gcttttttct
    13501 gaggactacg ccagcctcgg cacctttttc ctgttgtttg tgcaactggt tcccgcgttt
    13561 tgtctgataa ccattctgtt actggtgaat cgcgcggaat taccggtccg tttaaaagag
    13621 caactggcac gggtgctttt tcaactcaaa acctggggaa tggcggagat tttcctcgcg
    13681 ggtgtgctgg tcagtttcgt taaactgatg gcttacggca gcattggggt aggcagcagc
    13741 tttctcccct ggtgtttatt ttgtgtcctg caactgcgcg cttttcagtg cgttgatcgt
    13801 cgctggttat gggacgacat cgccccgatg ccagaactgc gccagccgct aaaaccaggc
    13861 gtcacgggga tacgtcaggg gctgcgttcg tgctcctgtt gtacggcaat ccttcctgct
    13921 gatgaacccg tgtgcccgcg ttgtagtacc aaagggtacg ttcggcgtag aaacagcctg
    13981 cagtggacac tcgcgctgct tgtaacgtcc atcatgctgt atcttccggc taatattttg
    14041 cccatcatgg tgacggattt attaggctcg aagatgccgt cgacgattct cgctggggtc
    14101 attctgttat ggagcgaagg ctcttatccc gtcgctgtgg tgatctttct ggccagtatt
    14161 atggtgccaa cgttaaagat gagcgccatc gcgtggctgt gttgggatgc caaagggcat
    14221 ggcaagcgcg acagtgaaag aatgcatttg atttatgaag ttgttgagtt tgtaggccgc
    14281 tggtcgatga ttgacgtttt cgttatcgcg gtgctctcgg cgctggtgcg tatgggaggt
    14341 ttaatgagta tttatccggc aatgggtgca ttaatgtttg ctttagtcgt cataatgaca
    14401 atgttttctg ctatgacgtt tgacccgcgt ttgtcgtggg atcgtcaacc tgaatcagag
    14461 catgaggagt cctgacagta tggaatctaa taatggggaa gccaaaatcc agaaagtgaa
    14521 gaactggtct cccgtgtgga tatttcctat cgtcacggcg ctcattgggg cctgggttct
    14581 tttttatcat tacagccatc agggaccgga agtgaccctg atcaccgcga atgcggaagg
    14641 aattgaaggt ggcaaaacca ccattaaaag ccgtagcgtt gacgtcggcg tggttgaaag
    14701 cgccacactg gctgatgatt tgacgcacgt tgaaatcaaa gcgcggctga attccggtat
    14761 ggaaaaattg ctgcataaag acaccgtctt ttgggtggtg aaaccgcaga ttggtcgcga
    14821 agggattagc ggcctgggaa cgctgctgtc tggagtttat atcgaactgc agccaggcgc
    14881 gaaaggcagc aaaatggata aatacgattt gctggactca ccaccgttgg ccccgcctga
    14941 tgcgaaaggt atccgtgtga ttctcgatag caaaaaagcc gggcagctct cgccaggaga
    15001 tccggtgctg ttccgtggct atcgggtagg ttcggttgaa accagcacct tcgatacgca
    15061 aaaacgcaat atcagttatc aactgttcat caatgcacct tatgaccgac tggtgaccag
    15121 caatgttcgc ttctggaaag atagtggcat tgcggttgat ctgacgtcag cggggatgcg
    15181 tgtggagatg ggctcattga caacgctgct tagtggcggt gttagctttg atgtgccgga
    15241 aggtctggat ttagggcagc cagtggcacc gaaaacagct ttcgttttgt atgatgatca
    15301 gaagagcatt caggattcgt tgtacaccga tcacattgat tatctgatgt tctttaaaga
    15361 ttcggtacgc ggtctgcaac cgggagctcc ggtagaattc cggggtattc gcctgggtac
    15421 cgtaagcaaa gtgccattct ttgcgccgaa tatgcgtcag acatttaacg atgattaccg
    15481 tattccggta ctgattcgta tcgagccaga gcggctgaaa atgcagcttg gcgaaaatgc
    15541 ggatgttgtt gagcaccttg gcgaattgtt gaaacgtggt ttacgcggat cgctgaaaac
    15601 cggaaacctg gtcactggtg cactgtatgt tgatctcgat ttctatccaa atacgcctgc
    15661 aataaccggt attcgtgaat ttaatggtta tcagattatc ccgactgtta gcggcggcct
    15721 ggcgcaaatc cagcaacgac tgatggaagc gttggataag atcaacaaac tgccattgaa
    15781 tccgatgatt gaacaggcaa ccagtacgct ttctgaaagt cagcgcacaa tgaaaaacct
    15841 gcaaacgacg ctggatagca tgaacaagat cctcgccagc cagtcgatgc agcagttgcc
    15901 gacggatatg cagtcaacgt tgcgtgaatt gaatcgcagc atgcagggct tccagcctgg
    15961 ctccgcagcc tacaacaaga tggtggcgga tatgcagcgc cttgatcagg tgttgcgaga
    16021 actgcaaccg gtgctgaaaa cgctcaatga gaagagtaac gcgctggtat ttgaagcgaa
    16081 ggacaaaaaa gatccagagc cgaagagggc gaaacaatga aaaagtggct agtgacgatt
    16141 gcagcactgt ggctggccgg atgcagctcc ggcgaaatta ataaaaacta ttaccagtta
    16201 cctgtggtgc agagcggtac acaaagtacc gccagccagg gcaatcgtct gttatgggta
    16261 gagcaggtca ctgttcctga ctatctggcg gggaatggtg tggtttatca aaccagtgat
    16321 gtgaagtatg tgattgccaa caacaacttg tgggccagcc cgttggatca acagttgcgc
    16381 aacaccctgg ttgccaacct gagtacgcaa ctgcccggct gggtggttgc ctcccagcct
    16441 ctgggaagcg cccaggacac gctcaatgtt accgtaacgg agtttaacgg tcgctatgat
    16501 ggcaaggtca ttgtcagtgg tgagtggctg ttgaaccacc agggacaact gatcaaacgt
    16561 ccgttccgtc tggaaggagt gcaaactcag gatggttacg atgagatggt taaagtgctg
    16621 gccggtgtct ggagtcagga agccgcttct attgcacaag agataaagcg tctaccttaa
    16681 ttataaagat ttgtaaatat aaccgtctcc ggtatgccgc ctgaggcggt ttttttgttt
    16741 ctaacgtgcg gaaaaatttg ttcctcttca cattttttgt acaaccgaca tgcccgtgta
    16801 gctcacaaat atgacagtgg cgtgaatttt gcgcattgac ggcagttatg attcgcggta
    16861 ttgcttaact gtgattgcac atttagtaat cactgttttc ttttccacca gaaaccagta
    16921 tgagggaaac gaggcatgaa gagacaaaaa cgagatcgcc tggaacgggc acatcaacgt
    16981 ggttatcagg ccggcatcgc cggacgctca aaagaaatgt gtccctatca gacgctgaat
    17041 caaaggtcac aatggctggg aggctggcga gaagccatgg cggacagggt agtaatggcc
    17101 tgattctgtc tctttaaaaa gaaacctccg cattgcggag gtttcgcctt ttgatactct
    17161 gtctgatgat aatcagaagg cagacgtatc ctggaacaga ccgactttca ggtcgctggc
    17221 ggtatagatc agacgacaat caaccagcac ttcgccatct gccagaccca taatcagacg
    17281 acgattaaca atgcgtttaa agtgaatacg gtaggtcact tttttcgctg tcggcagtac
    17341 ctgaccagtg aatttcactt cgccaacgcc cagcgcgcgg cctttacctt cgccgcccag
    17401 ccagccgagg tagaacccta ccagctgcca cattgcgtcc aggcccaggc atcccggcat
    17461 aaccggatcg ccaatgaagt ggcatccgaa gaaccacaga tccggattga tatccagttc
    17521 tgcttcaaca taccctttgt cgaagttacc acccgtttcg gttattttga ccacacggtc
    17581 catcatcagc atgttcggtg ctggcaattg cgggccttta gcgccaaaca gttcaccgtg
    17641 accagaggca agaaggtctt cttttgtata ggattcgcgt ttatctacca tgttctctgt
    17701 aagccttatt ttattgaagc acgcaggata gctaacacgt gtacgctgaa caagtccgat
    17761 cagttcggaa taaaccagtt cagccaacgt aatggccatg gaaaacggtg acgtccttcc
    17821 tgttgcgatg cttgcgcgat acgttcctgg atggtttgca tcagcgtcgt ttggccttcg
    17881 ccatcccaca ccagatttaa taataacggc agtgcgtcag tcacatcgtc tactgcccag
    17941 atggtgaatt tgccttcttc taccgctttc accagttcac tgtgaagact taaatggcga
    18001 acgttagcag tggggataat gacaccttgt ttccccgtta actcacgttg ctggcaaata
    18061 gcaaagaagc cttcgatttt ctcatttaaa ccaccgaccg gctggacgcg gccgaactga
    18121 tcgactgaac ctgtgatagc gatactctga ttcactggca catcagcgag ggcgcttatc
    18181 agggcgcaga gttcagccat cgaggcacta tcgccatcca cttcactgta tgactgctca
    18241 aatgtcagcg atgctgagaa ggggatctgt tgctcaagct gtagttccga catcaggaac
    18301 gcctgcatga tcatcatccc tttcgcatgg atattgccgc caagctccgc tttgcgttcg
    18361 atgtcggtga attcaccatc gccaatatgc acaacgcagc taatgcgaga aggttcgcca
    18421 aaagcgcgtg gatgacccgg aaattcaatg accgaaaggg cgttaatttg cccgatgcgt
    18481 tcgccttcgg tttcaatcag gatttgctcc tgaaggatct catcctgcat acgttcagcg
    18541 aggaaacctt cgcgccattc acgctgctgc agcattaagt ttagctgctc gccggagaag
    18601 gtgtcgccat cacacaggga ggcgacctct ttacactggc ggaggatcca atgcgggcta
    18661 agcggaagtg tttcttgttc accggtgtag cgtgctgctt cgcggataag taccggccaa
    18721 gcatccgctc ccggggcagg taagtgatta tgtctggcgg taaatgtcac ccagcgacac
    18781 cactggctta ctgactccgc atcgacaatc tgcagagtat cttcaaattc gctataaata
    18841 gcctgctctg aaagctctgg ctccatctcc tggaaatcag ccaatgattc gcgttcgcct
    18901 accagaatga ctttcagctt caatggcatc gaaggcacag agacggggag agggcgcgac
    18961 tcatcaaacg caacccagtc aaaacgctcg cggttaacga tatttttcag ccgcatccac
    19021 agcagaggtt gcgccagcag tgtacgcaaa gagatgatga gaataccgcc atttgcctga
    19081 tgcaccagac caggctgcag ggtaatgtcg ccattaaact ggcgcaggca gccaaagagt
    19141 tgctccgctt ctacccagtc ggcagcgaca acttgcgtta aagtcgcaaa attatcatct
    19201 gcactcactg cgtgacgtaa gcggatggag tggccggaaa cctcatagtg atcgcccacc
    19261 agttggcctg catcgctttg tagcgtacgc gcggcatcgg caataagatt cagatactca
    19321 gactcttccg gggccttagc cagcataaaa gaggaggatg ctcgcgtatg cagcaactgc
    19381 tccagcgcaa attgcagtcg cggttgagta tcactgaata aaggatcgtt ttcgtcaatc
    19441 aaatgtggct gagcaaatat ttcctgatag ctatcggtat caggaaccag gtcacgccat
    19501 gcaagtttcg taatggtcaa agttgatgtt ttttagtctg ttgtcaaagc cgcgattata
    19561 ccgtaaccgg cactacagca cacgtagaaa gcaccgacaa tactcctggc tgggcgttaa
    19621 agctcacagg atggagattc ttttcttcac tggcctaaaa agctgatatt ctgtaaagag
    19681 ttacacggta acattgagat cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt
    19741 tggaaatgga agtatctggt aaagaagcat cgcgaagggg agttaattac ccgttacata
    19801 gaagccagtg ccgcccagga agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg
    19861 ctggtaaatg gctggattga caagcatatg aatccggaac tggtcaatcg catgaagcag
    19921 accattcggg ccagacggaa gcgccatttt aatgcggaac accagcatac gcgcaaaaaa
    19981 tcgatcgatc tggaatttat tgtctggcaa cgtctggctg gtcttgcaca gcgtcgcggt
    20041 aaaacgcttt ctgaaacgat tgttcagctg attgaagatg cggaaaacaa agagaaatac
    20101 gcgaataaaa tgtcttctct gaagcaggat ctgcaggcat tgctgggtaa ggaataactg
    20161 acgaaagtca gttcaattta ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta
    20221 ccagacgata acttaagcct gcggctgagt tacaacgtct ttgatacctt taacttcgat
    20281 ctctacgcga cgatccggag ccaagcagtc gatcagtgca gcacgctgtt tcacgttgtc
    20341 acaggtgttg ccagtaaccg ggttggattc gcccatacca cgtgcggaga tcttgtctgc
    20401 cgggatacct ttggagatca ggtaatcaac aacagactga gcacggcgct cggacagacc
    20461 ctggttgtaa gcgtcagaac cgatgcggtc agtgtaaccc agaacaacta cggaaccgtc
    20521 tttcggatcc aggttgctca gctggctgta cagctgatcc agagcagcct gaccttccgg
    20581 tttcagggtt gctttgttga agttgaacag aacgtcagac ttcagagtga agtgcttggt
    20641 ctgtacttcc ggtgccggag ctggagcaac taccggagct gcttcgccct gaccgaaacg
    20701 gtaggaaaca cccaagctca gcagaccgtt gtccggacga gtaccgatgg tgtttgcgtc
    20761 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    20821 ctcaacacca cctgcgaaaa ccggagaaac gccagtgtcg tggtctttaa aggatgcgcc
    20881 accaggtacg ttagccttgg tgtctgcacg ccataccata ccacccagac gagtgtagat
    20941 gtccagatcg tcagtgattg ggtaacccag tttagcggtc agctgaacgc cctgagcttt
    21001 gtatgcgccg ttgatgttgt cgcctttgta cggcatacga cctaaccagt cgtaacccat
    21061 ttcaaaacca acatacgggt taacctggta accaccaaaa gcacctgcac ccagttggtt
    21121 ttcgtgggtc ggaccattgt taggaataaa accagtgtca tggtactggg accagcccag
    21181 tttagcacca gtgtaccagg tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc
    21241 agccagtgcc actgcaatcg cgatagctgt ctttttcatt ttttgcgcct cgttatcatc
    21301 caaaatacgc catgaatatc tccaacgaga taacacggtt aaatccttca ccgggggatc
    21361 tgctcaatat taactctacc gatatcttcg gcttatgccg agcacccctg gcgatgtaaa
    21421 gtctacaacg tagttgaaaa cttacaagtg tgaactccgt caggcatatg aaaaaaaagt
    21481 cttgtataag gtatgtttaa tctttttgtc agcgacaatt tacagaaaaa aaacgcggaa
    21541 accgcttcag acaagccttc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
    21601 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
    21661 ttagcccgga aagttgtctc gttgtgtgag aggatgcgct taccggacgc ataataaacc
    21721 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
    21781 catctgtcaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
    21841 actccactgt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
    21901 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
    21961 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
    22021 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
    22081 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
    22141 acataatcaa tccagcccct gtgagttact gtatggatgt gcagtacatc cagtgacaac
    22201 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
    22261 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
    22321 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
    22381 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
    22441 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
    22501 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
    22561 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
    22621 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
    22681 ctgaaagaga aaagcacgcg caatacccgg gaaagactga aagatttgcc caatatgtct
    22741 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
    22801 cttggggcaa aaatgtgttg gttgcgactg cggcagcaaa acagtctggt gacagaaaag
    22861 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
    22921 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
    22981 gagcttgagt aatctggcgt tgcagacgac caatctctgg cagtaatgca atcaataacc
    23041 caacttgttg tacgaccaga ggttctttgc tatctgcccg tggttcaagt tgctgcatcc
    23101 gctgtttcag gccagctaat gcctgattga cgcgttcttc atcagcaggt tgatgatgta
    23161 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
    23221 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
    23281 gcaagcgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggttcgctg gacatatttg
    23341 ataccacaga cgccagttca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
    23401 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga tagcgacagt
    23461 tggcctctgt ggcgcgttcg agcattcgcg gcagattgcg aaacttccag tcgggccaga
    23521 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
    23581 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
    23641 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
    23701 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatcg
    23761 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
    23821 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
    23881 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttaccgca tgacggaaga
    23941 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc aatccgtgcg
    24001 ggctgtcatc agcgagctca ttttcgtcat tattacgggg tagtgcctgg gcctgttctg
    24061 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
    24121 ttttgagtaa atcggcgggt gcgccgttat cgcgtatccg ctccagcgca gcatcaatat
    24181 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataaga ctgacgcaac aaaatacagc
    24241 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
    24301 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
    24361 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
    24421 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
    24481 gctgaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
    24541 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
    24601 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
    24661 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
    24721 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
    24781 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
    24841 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
    24901 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
    24961 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
    25021 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccaag
    25081 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
    25141 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
    25201 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
    25261 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
    25321 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcaacaca tttttgccag
    25381 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
    25441 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
    25501 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
    25561 aaacggtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
    25621 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
    25681 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
    25741 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
    25801 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
    25861 gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac agaatggggc
    25921 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
    25981 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
    26041 gaaaataaat ggctgacgcg tgagcaaacc tctgacgttc agcaacaaat ccgccaggct
    26101 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
    26161 tggcgtaaat gtcaggcttg gttgaaagat attgaaagcg ctcggttgca gcataaccag
    26221 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
    26281 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
    26341 gccggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
    26401 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
    26461 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
    26521 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
    26581 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
    26641 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
    26701 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
    26761 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
    26821 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
    26881 ttaaaagcct ggaaaggtgc gctgaaggca gaaaacgctg tcgatttttc gggccttatt
    26941 catcaggcga ttgtgattct ggagaaaggc cgctttatca gcccgtggaa gcatattctg
    27001 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
    27061 caaaacagtc agacgacgtt gttcgccgtt ggtgatgact ggcaggcgat ttaccgattc
    27121 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgatcgc
    27181 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
    27241 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
    27301 aaagctgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
    27361 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
    27421 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
    27481 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga ggggagtgat
    27541 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
    27601 gatttcccgg acgctgaaga acggcgttta atgtacgtgg cgctgacccg ggcacgccat
    27661 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
    27721 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
    27781 attacttcag acggtcagcg agataacgct gataatcggg gataagaata tcgaccgcgt
    27841 cgttgaaatg cggcgactgg ataatgaagt ctgccgttgc cacgttggtg gcgaccggaa
    27901 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
    27961 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
    28021 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
    28081 gggaaatcaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
    28141 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
    28201 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
    28261 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
    28321 acaggcacaa aaatctcagg aagatggttg tttttccgcc cattgcagga aagtatttcg
    28381 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
    28441 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
    28501 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
    28561 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
    28621 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
    28681 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
    28741 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
    28801 cgtcttctca acgcgaaaca ctaactgatg cggtccattg tccagttcaa tgctgtccgc
    28861 accgcgcagc agagagctgg aaaccttttt gccgtcgagt accagaagat cgacatcggt
    28921 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
    28981 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
    29041 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
    29101 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
    29161 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
    29221 gctggtgggg gcaagcgaca aacccgatcg ccccagctat cgcgtgatga aatatctact
    29281 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
    29341 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
    29401 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct attgccattg gcgcgaaaac
    29461 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
    29521 gttaaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gtctggccaa
    29581 ataaaaaatc cccggaaggc aaaaaccttc cggggatttg ttcagggatt agttacgcag
    29641 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
    29701 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
    29761 taggccgtta tcgtcctcca tcaccacgtg ataccacgga gcggcgcgaa gctcgtcatt
    29821 caccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
    29881 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
    29941 aatcatagtc acctcccggg aaatctttac ctttgatata agggtaaaat tccacatttc
    30001 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccgggt aggtagcgat
    30061 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac gaccggcatc
    30121 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
    30181 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatgtcttt
    30241 ataaccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
    30301 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
    30361 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
    30421 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
    30481 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
    30541 gtagcggcgg gtagccaggc ggctgtctcg ctggtccaga tagtagcccg ttttgtgtcc
    30601 gtgctgaata tccaccagca gcttcatgcc gtgttcttca atcggcagca gggcaggcgg
    30661 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
    30721 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
    30781 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
    30841 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
    30901 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
    30961 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
    31021 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
    31081 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
    31141 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
    31201 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
    31261 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctggattta
    31321 cgggcgagtt cagggcgtag gatttcgcta caccacacag tacgaagcga aaagactggg
    31381 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
    31441 agaagggcag gtagaaaaat taatgcagtg gttaaagagt ggcggcccgc gttctgcgcg
    31501 tgtagaacgg gtgctcagcg agccgcatca tccctcgggg gaattaaccg attttcgtat
    31561 ccgttaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    31621 gcctttcggg aacagtcggt ataaatagcg gctgttacct ttttcttcac caaatttatt
    31681 cgccatcgct tttaccagca tacgaatcgc cggagacgta ttaaattcca ggtagaagtc
    31741 acgtacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    31801 aatcaccact gccagcggtt cactccactg gctgctttct ttgagatagc cttcggtatc
    31861 cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    31921 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    31981 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    32041 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    32101 gctggtttca aacaagatag cgccagagga gatcagaatg aagaccgcgc tgatcgccag
    32161 atgcagagca ggcagttgca ggaagatatt agcaaccata ccaatcagta ccaccacaat
    32221 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    32281 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    32341 tccggcagac agataggtgt tcagaattgg tccgaggata taacccagaa aaccggtaaa
    32401 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    32461 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    32521 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    32581 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    32641 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    32701 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    32761 tatcaggtca ttaagcaaat ataacgccct gagaattccg acaggcaaaa gaaaaagggg
    32821 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    32881 ccctttcggg tcgccggttt tcaagaccgg tgccttcaac cgctcggcca cacttccgga
    32941 atgacgcgca ctataaacat cccgatgcgg cgtgtaaacc cctaatttgt ttgtttgcct
    33001 gaaaaacagc caaaagtgca ttgatagcgt gaaataacag cagattgatc atttcatcac
    33061 catgaattcc ttctctttta ctcgtttagc aaccggctaa acatccccat cgcccggcta
    33121 aagaaaaata ggtccatttt tatcgctaaa agataaatcc acacagtttg tgttgttttg
    33181 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgtgc cagtgcagaa
    33241 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    33301 tcgcttgccg accataagcg cccggtgtcc tgccggtgtt gctaggagaa gagacgtgcg
    33361 atatgaataa cgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    33421 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    33481 caaagattgc ctgggcgctg gagaacaaac cgcgcattcc ggtggtatgg atccacggtc
    33541 tggaatgtac ctgctgtacc gaatctttta tccgctccgc tcacccactg acgaaggacg
    33601 tcatcctttc cctgatttcc ctctattacg acgataccct gatggctgcc gccggaaccc
    33661 aggcggaaga agtctttgaa gacatcatca cgcaatataa tggcaaatat atcctcgcag
    33721 tagaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    33781 ttattgagaa actcaaacgt gccgctgccg gagccagcgc gattatcgcc tggggaacct
    33841 gcgcgtcctg gggctgcgtg caggccgcgc gacctaatcc gacacaggca acgcctatcg
    33901 acaaagtcat caccgacaaa cccattatca aagtacctag ctgcccgccg atcccggatg
    33961 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    34021 tgggtcgtcc actgatgttt tatggtcagc gtatccacga taaatgctat cgccgcgctc
    34081 acttcgacgc cggagagttc gtccagagtt gggatgatga cgctgcccgc aaaggttatt
    34141 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    34201 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggc tgtgcggaaa
    34261 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattccg caaatgggta
    34321 ctcattccac cgccgatacc gtcggtttaa ccgcgcttgg cgtggtggca gcagctgttg
    34381 gtgtgcacgc agttgccagc gccgttgacc aacgcagacg tcataaccag caacctacag
    34441 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    34501 gggatacacc atcaataatg ccggacgccg cctggtggtc gacccgatta cgcgcatcga
    34561 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    34621 ctgcggcacc atgtttcgcg ggctggagat catcctgcaa gggcgcgacc cgcgcgatgc
    34681 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    34741 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    34801 cattatgctg acaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    34861 gatggactgg atcgacgtgt tagatgcgct gaaagccgac ccgcgaaaaa cctccgaact
    34921 ggcgcaaagt ctctcctctt ggccgaaatc atcccctagc tatttcttcg acgtacaaaa
    34981 tcgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    35041 gcacccgcag tacaaattgc cgctagaagc caacctgatg ggctttgccc actatctcga
    35101 agctctcgat ttccaacgtg aaattgtcaa aattcacgcg gtctttggcg gtaaaaaccc
    35161 gcatccaaac tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    35221 ggtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgattatca cccgcacggc
    35281 agacttcatt aacaacgtga tgatccccga cgccttagcc atcggtcagt tcaacaaacc
    35341 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc
    35401 ggatattgcc aacgactttg gtgagaaaag tctgctgatg cctggcggcg cggtgattaa
    35461 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    35521 tgtcgatcac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cattcgatgg
    35581 cattaccgac ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    35641 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    35701 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    35761 tgagtcggtc gatcgcatga tgtcggcgtt gaacctgccg ctttccggta tccagtcaac
    35821 gttgggccgc aggtaagttg cagtatttct tcgacaagct gatgaccaac ctgaaaaatg
    35881 gcaatctcgc cactgcttcc actgaaaaat gggaacctgc aacctggccg acagagtgcc
    35941 gtggcgtcgg ctttaccgaa gcgccgcgcg gagcgttagg ccactgggcc gccattcgcg
    36001 atggcaagat tgatctctac cagtgcgtgg tgccgaccac ctggaacgcc agcccgcgcg
    36061 atcccaaagg gcagattggc gcttatgaag cggcgctgat gaacaccaaa atggcgatcc
    36121 ccgagcaacc gctggagatc ctgcgtactc tgcacagctt tgatccgtgc ctcgcctgtt
    36181 caactcatgt gctgggcgac gacggtagcg agctgatctc cgtgcaggtg cgttaacagc
    36241 gaaggagaat catcatgcaa cagaaaagcg acaacattgt cagccactat gtctttgaag
    36301 caccagttcg tatctggcac tggctgacgg tgttatgcat ggcggtgttg atggtcaccg
    36361 gttactttat cggcaagccg ctaccttccg tcagtggcga ggcgacgtat ctgttctata
    36421 tgggttacat caggttaatt cacttcagcg ccgggatgat ttttaccgtg gtcttgctga
    36481 tgcggatcta ctgggctttt gttggcaatc gttactcccg cgagctgttt atcgtgccgg
    36541 tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat ccgctggtat ctgtttctgg
    36601 caaaacgtcc gagtgccgat ataggccata accccatcgc ccaggcggcg atgttcggct
    36661 atttcctgat gtcggtcttt atgatcatca ccggttttgc gctgtacagc gaacacagcc
    36721 agtacgctat ttttgcgccg ttccgttatg tggtggaatt tttctactgg acgggtggca
    36781 actcaatgga cattcacagc tggcatcggc tggggatgtg gctgattggg gcgtttgtga
    36841 tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc cgacgacacg gtgatctcca
    36901 ccatggtcaa cggctaccgt agccacaaat ttggcaaaat aagtaacaag gagcgttcat
    36961 gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg ctgtgggccg atgaaggctt
    37021 cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac tggcccgagt atgtggagat
    37081 tgtcgatggc ggtactcagg gactgaactt gctggggtat gtcgaaagcg ccagccatct
    37141 gttgattctc gatgccattg actacgggct ggaacctgga aggctgcgaa cctatgccgg
    37201 agaacgcatt ccggcctatc tcagcgcgaa gaaaatgagc ctgcatcaga acagtttctc
    37261 cgaagtgctg gcgctggcag atatccgcgg acatctgcca gcacatattg ccctcgtcgg
    37321 gctgcaaccc gcgatgctcg acgactacgg cggcagcctg agcgaactgg cacgggagca
    37381 actgcccgct gcggaacaat cggcgctggc gcagttggct gcgtggggaa gtgtgccgca
    37441 accggctaat gaatcgcgtt gtctcaatta tgactgtctg tcgatggaaa attacgaagg
    37501 cgttcgcttg cgccagtacc ggatgacaca ggaggagcag ggatgagcaa cgacacgcca
    37561 tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga cgccggtcag tgaatcccgt
    37621 cttgacgact ggcttacgca agcgccagac ggcgtggtgt tattaagcag tgacccgaaa
    37681 cgcacgccag aggtcagcga taatccggta atgattggcg aattactgca cgagtttccc
    37741 gactatacat ggcaggtggc gattgctgac cttgagcaga gcgaagccat cggcgatcgc
    37801 tttggcgcct ttcgctttcc tgccacttta gtgtttaccg gcggaaacta tcgcggcgtg
    37861 ctgaatggta ttcacccgtg ggcggaactg ataaacctga tgcgcgggct tgtcgaaccg
    37921 cagcaggagc gtgcctcatg agcgaaactt ttttccatct gctggggcca ggaacgcaac
    37981 cgaacgatga cagtttcagc atgaatccac tgccgatcac ctgtcaggtg aatgatgaac
    38041 cgagtatggc ggccttggag caatgtgctc acagcccgca ggtgattgcg ctgttaaacg
    38101 agttacaaca tcaactaagc gaacgccagc cgccgttggg cgaggtgctg gcagtcgatc
    38161 tgttaaatct caacgccgac gatcgtcact ttatcaatac gcttctcggg gaaggggaag
    38221 tgtcagtgcg cattcagcag gctgacgaca gtaaaagtga aatacaggag gcgatcttct
    38281 gcggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt gctggaggac aaactggagg
    38341 ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca aaatctcttg ccgacagatt
    38401 cgctgttacc gccgcccatt gatggcctga tgaatggcct accgttggcg catgagttac
    38461 tggcacatgt acgtaacccc gacgcgcagc cgcacagcat taatctgacg caattaccca
    38521 tcagcgaggc tgatcggctt tttctctcac gtctctgtgg gccgggaaat attcagattc
    38581 gtaccattgg ctatggcgag agctatatca actccacggg gttacgccat gtctggcatt
    38641 tacgctgtac ggacacctta aaaggcccgt tactggaaag ttatgaaatc tgcccaatac
    38701 cggaagtggt gctggcagcg ccagaagatt tggtcgactc tgcgcagcgg cttagcgagg
    38761 tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga cggtaagtat cgctttcagt
    38821 cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga gtttcaggat aaagagggag
    38881 atctaacatt atcgggttat ttttctctct tcgcctacag gagtgcgcat gtgggatgtc
    38941 attgatttat cgcgctggca gtttgctctg accgcgctgt atcacttttt atttgtaccc
    39001 cttaccctgg ggctgatttt tttgctggct atcatggaaa ccatttacgt ggtcaccggc
    39061 aaaacaatct accgcgatat gacgcgcttc tggggtaagc tcttcggtat caattttgct
    39121 cttggcgtgg ctaccggcct gaccatggag tttcagtttg gtactaactg gtcattctat
    39181 tccaactatg tgggcgatat ttttggcgca ccactggcga tggaagcatt aatggccttc
    39241 ttcctcgaat ccacctttgt cgggctgttc ttcttcggct ggcaacggct gaataaatac
    39301 cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa atctctctgc attgtggata
    39361 ttgaatgcca acggttggat gcaatacccg accggtgcgc attttgatat cgacaccctg
    39421 cgtatggaga tgaccagctt cagcgagctg gtctttaacc cggtcagcca ggtgaaattt
    39481 gtgcacaccg tcatggcggg ctacgtgacc ggggccatgt ttattatggc gatcagcgcc
    39541 tggtatttac tgcgcggacg ggagcgcgat gtcgcattac gctcgtttgc catcggttcc
    39601 gtcttcggta ctctggcgat tatcggtacc ctgcaactcg gagacagttc tgcgtatgaa
    39661 gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag gggagtggca aacggaacct
    39721 gctcctgcac cgttccatgt ggttgcctgg ccggaacagg atcaagagcg taacgccttt
    39781 gccatcaaaa ttcccgcgct gctagggatc ctcgccactc actcattaga taaacccgtg
    39841 ccgggtctga agaatttgat ggctgaaacc tacccacgct tgcaacgcgg acgtatggcc
    39901 tggctgttaa tgcaggaaat atctcaaggc aatcgtgagc cgcatgtgtt gcaggcattc
    39961 cgggagctgg aaggcgacct tggttacggc atgttgctct cccgctatgc gccggatatg
    40021 aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg gcgcgatacc tcaggttgcg
    40081 ccggtattct ggagtttccg catcatggtg ggctgtggtt ccctgctgct actggtgatg
    40141 ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc agcatcgctg ggtgctgaaa
    40201 atggcgctct ggagtttgcc gttgccgtgg attgcgattg aagccgggtg gtttatgacc
    40261 gagtttggtc gtcagccgtg ggcgatacag gacatcttac cgacatactc cgcgcactcc
    40321 gctttaacca caggacaact ggctttctca ctgatcatga tcgtaggtct ttacaccctg
    40381 ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc gtctggggcc gagcgcgatg
    40441 cagagtgaac aaccgacgca gcaacagggg taaaggagaa aatcatgttt gattatgaaa
    40501 cattgcgctt catctggtgg ctgctgattg gcgtgatcct ggtggtcttt atgatctccg
    40561 acggatttga catggggatc ggctgtttgc tgccgctggt ggcgcgtaat gatgatgaac
    40621 gccggatagt gattaatagc gttggtgcgc actgggaagg caatcaggtc tggttgatcc
    40681 tcgcaggtgg ggcattattt gccgcctggc ccagagtgta tgcagcggcg ttttccggct
    40741 tttatgtggc gatgatcctg gtgctgtgct cgctgttctt ccgtccgctg gcctttgatt
    40801 atcgcggaaa aattgccgat gcacgctggc gtaaaatgtg ggatgccggt ctggtcatcg
    40861 gcagtctggt gccgccggta gtcttcggta tcgccttcgg caacttgttg ctcggcgtgc
    40921 cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg cagcttctgg caactgctga
    40981 cgccattccc tttattgtgt ggtttgctca gccttgggat ggtgattttg caaggtggcg
    41041 tctggttaca actgaaaact gttggtgtga ttcatctgcg ttcacagctg gcgactaaac
    41101 gcgctgcact gttggtgatg ctgtgctttt tgctggcagg ttactggctg tgggtcggta
    41161 ttgatggctt tgtactgctc gcccaggatg ctaacggtcc ttccaatccg ttaatgaaac
    41221 tggtggcagt gctacctggt gcctggatga ataatttcgt cgaatcgccc gttttgtgga
    41281 tcttcccgct gctggggttc ttctgcccat tgctgacggt gatggcgatt tatcgtggtc
    41341 gcccgggttg gggatttttg atggcatcat tgatgcaatt tggcgtgatt ttcacggcag
    41401 gcatcacgct gttccccttt gtcatgccgt caagtgtgag tccaatctcc agcctgacgt
    41461 tgtgggacag cacttccagt cagctgacgc tgagcattat gttggtaatc gtgctgatat
    41521 ttttgcccat tgtgttgctc tacactctct ggagctacta caaaatgtgg ggacgcatga
    41581 caacagaaac tctccgccgt aacgaaaacg agttgtacta aggagcagga acatgtggta
    41641 tttactttgg ttcgtcggca ttttgttgat gtgttcgctc tccacccttg tgttggtatg
    41701 gctggacctg cgtctgaaaa gttaacgaac gtaggcctga tgcggcgcat tagcatcgca
    41761 tcaggcaatc aataatgtta gatatgaaaa gcggaaacat atcgatgaaa gcgatcttaa
    41821 tcccattttt atctcttctg attccgttaa ccccgcaatc tgcattcgct cagagtgagc
    41881 cggagctgaa gctggaaagt atggtgattg tcagtcgtca tggtgtgcgt gctccaacca
    41941 aggccacgca actgatgcag gatgtcaccc cagacgcatg gccaacttgg ccggtaaaac
    42001 tgggttggct gacaccgcgc ggtggtgagc taatcgccta tctcggacat taccaacgcc
    42061 agcgtctggt agccgacgga ttgctggcga aaaagggctg cccgcagtct gctcaggtcg
    42121 cgattattgc tgatgtcgac gagcgtaccc gtaaaacagg cgaagccttc gccgccgggc
    42181 tggcacctga ctgtgcaata accgtacata cccaggcaga tacgtccagt cccgatccgt
    42241 tatttaatcc tctaaaaact ggcgtttgcc aactggataa cgcgaacgtg actgacgcga
    42301 tcctttgcag ggcaggaggg tcaattgctg actttaccgg gcatcggcaa acggcgtttc
    42361 gcgaactgga acgggtgctt aatttcccgc aatcaaactt gtgccttaac cgtgagaaac
    42421 aggacgaaag ctgttcatta acgcaggcat taccatcgga actcaaggtg agcgccgaca
    42481 atgtctcatt aaccggtgcg gtaagcctcg catcaatgct gacggagata tttctcctgc
    42541 aacaggcaca aggaatgccg gagccggggt ggggaaggat caccgattca caccagtgga
    42601 acaccttgct aagtttgcat aacgcgcaat tttatttact acaacgcacg ccggaggttg
    42661 cccgcagtcg cgccaccccg ttattggatt tgatcatggc agcgttgacg ccccatccac
    42721 cgcaaaaaca ggcgtatggt gtgacattac ccacttcagt gctgtttatt gccggacacg
    42781 atactaatct ggcaaatctc ggcggcgcac tggagctcaa ctggacgctt ccaggtcagc
    42841 cggataacac gccgccaggt ggtgaactgg tgtttgaacg ctggcgtcgg ctaagcgata
    42901 acagccagtg gattcaggtt tcgctggtct tccagacttt acagcagatg cgtgataaaa
    42961 cgccgctgtc attaaatacg ccgcccggag aggtgaaact gaccctggca ggatgtgaag
    43021 agcgaaatgc gcagggcatg tgttcgttgg caggttttac gcaaatcgtg aatgaagcac
    43081 gcataccggc gtgcagtttg taatgcataa aaaagagcat tcagttacct gaatgctctg
    43141 aggctgatga caaacgaaga actgtctaat gcgtagaccg gaaaaggcgt tcacgccgca
    43201 tccggtcact ttcagtttta ctctttctcg gagtaactat aaccgtaata gttatagccg
    43261 taactgtaag cggtgctggc gcgtttaatc acaccattga ggattgcgcc tttaatatta
    43321 acgccagcct gttccagacg ctgcattgac aagctcacct ctttggcggt gttcaagcca
    43381 aaacgcgcaa ccagcaggct ggtgccgaca gagcgtccca cgaccgcagc atcgctcacc
    43441 gcca
//
LOCUS       DADLTF010000015        41045 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.015, whole genome shotgun sequence.
ACCESSION   DADLTF010000015 DADLTF010000000
VERSION     DADLTF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.015"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..570
                     /locus_tag="JN749_000814"
                     /pseudo
     CDS             1..570
                     /locus_tag="JN749_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
     gene            640..1416
                     /gene="yaaA"
                     /locus_tag="JN749_000815"
     CDS             640..1416
                     /gene="yaaA"
                     /locus_tag="JN749_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HAY7367317.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(1569..1865)
                     /locus_tag="JN749_000816"
     CDS             complement(1569..1865)
                     /locus_tag="JN749_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7367318.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2079..3365)
                     /gene="thrC"
                     /locus_tag="JN749_000817"
     CDS             complement(2079..3365)
                     /gene="thrC"
                     /locus_tag="JN749_000817"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HAY7367319.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(3366..4298)
                     /gene="thrB"
                     /locus_tag="JN749_000818"
     CDS             complement(3366..4298)
                     /gene="thrB"
                     /locus_tag="JN749_000818"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HAY7367320.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4300..6762)
                     /gene="thrA"
                     /locus_tag="JN749_000819"
     CDS             complement(4300..6762)
                     /gene="thrA"
                     /locus_tag="JN749_000819"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HAY7367321.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(6843..6908)
                     /gene="thrL"
                     /locus_tag="JN749_000820"
     CDS             complement(6843..6908)
                     /gene="thrL"
                     /locus_tag="JN749_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HAY7367322.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7122..7808)
                     /locus_tag="JN749_000821"
     CDS             complement(7122..7808)
                     /locus_tag="JN749_000821"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7367323.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8208..8348)
                     /locus_tag="JN749_000822"
     CDS             complement(8208..8348)
                     /locus_tag="JN749_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367324.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8444..9160
                     /gene="arcA"
                     /locus_tag="JN749_000823"
     CDS             8444..9160
                     /gene="arcA"
                     /locus_tag="JN749_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HAY7367325.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9219..10571)
                     /gene="creD"
                     /locus_tag="JN749_000824"
     CDS             complement(9219..10571)
                     /gene="creD"
                     /locus_tag="JN749_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HAY7367326.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10629..12053)
                     /gene="creC"
                     /locus_tag="JN749_000825"
     CDS             complement(10629..12053)
                     /gene="creC"
                     /locus_tag="JN749_000825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HAY7367327.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     VAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(12053..12742)
                     /gene="creB"
                     /locus_tag="JN749_000826"
     CDS             complement(12053..12742)
                     /gene="creB"
                     /locus_tag="JN749_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HAY7367328.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSLGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12755..13228)
                     /gene="creA"
                     /locus_tag="JN749_000827"
     CDS             complement(12755..13228)
                     /gene="creA"
                     /locus_tag="JN749_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HAY7367329.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13439..14308
                     /gene="robA"
                     /locus_tag="JN749_000828"
     CDS             13439..14308
                     /gene="robA"
                     /locus_tag="JN749_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HAY7367330.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14305..14952)
                     /gene="gpmB"
                     /locus_tag="JN749_000829"
     CDS             complement(14305..14952)
                     /gene="gpmB"
                     /locus_tag="JN749_000829"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HAY7367331.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15004..15525
                     /gene="yjjX"
                     /locus_tag="JN749_000830"
     CDS             15004..15525
                     /gene="yjjX"
                     /locus_tag="JN749_000830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HAY7367332.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15610..15936)
                     /gene="trpR"
                     /locus_tag="JN749_000831"
     CDS             complement(15610..15936)
                     /gene="trpR"
                     /locus_tag="JN749_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HAY7367333.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16026..17963)
                     /gene="sltY"
                     /locus_tag="JN749_000832"
     CDS             complement(16026..17963)
                     /gene="sltY"
                     /locus_tag="JN749_000832"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HAY7367334.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     EIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18174..19841
                     /gene="ettA"
                     /locus_tag="JN749_000833"
     CDS             18174..19841
                     /gene="ettA"
                     /locus_tag="JN749_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HAY7367335.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20149..21381)
                     /gene="nadR"
                     /locus_tag="JN749_000834"
     CDS             complement(20149..21381)
                     /gene="nadR"
                     /locus_tag="JN749_000834"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HAY7367336.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(21402..22784)
                     /gene="radA"
                     /locus_tag="JN749_000835"
     CDS             complement(21402..22784)
                     /gene="radA"
                     /locus_tag="JN749_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAY7367337.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22833..23801)
                     /gene="serB"
                     /locus_tag="JN749_000836"
     CDS             complement(22833..23801)
                     /gene="serB"
                     /locus_tag="JN749_000836"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HAY7367338.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23907..24551
                     /locus_tag="JN749_000837"
     CDS             23907..24551
                     /locus_tag="JN749_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HAY7367339.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24579..25595
                     /gene="lplA"
                     /locus_tag="JN749_000838"
                     /pseudo
     CDS             24579..25595
                     /gene="lplA"
                     /locus_tag="JN749_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            25627..25890
                     /locus_tag="JN749_000839"
     CDS             25627..25890
                     /locus_tag="JN749_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7367340.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(26051..26770)
                     /gene="deoD"
                     /locus_tag="JN749_000840"
     CDS             complement(26051..26770)
                     /gene="deoD"
                     /locus_tag="JN749_000840"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAY7367341.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(26827..28050)
                     /gene="deoB"
                     /locus_tag="JN749_000841"
     CDS             complement(26827..28050)
                     /gene="deoB"
                     /locus_tag="JN749_000841"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAY7367342.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(28102..29424)
                     /gene="deoA"
                     /locus_tag="JN749_000842"
     CDS             complement(28102..29424)
                     /gene="deoA"
                     /locus_tag="JN749_000842"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HAY7367343.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLDGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(29551..30330)
                     /gene="deoC"
                     /locus_tag="JN749_000843"
     CDS             complement(29551..30330)
                     /gene="deoC"
                     /locus_tag="JN749_000843"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAY7367344.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            30588..32129
                     /locus_tag="JN749_000844"
     CDS             30588..32129
                     /locus_tag="JN749_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HAY7367345.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDTRCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            32101..32964
                     /gene="yjjW"
                     /locus_tag="JN749_000845"
     CDS             32101..32964
                     /gene="yjjW"
                     /locus_tag="JN749_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HAY7367346.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(33178..33960)
                     /locus_tag="JN749_000846"
     CDS             complement(33178..33960)
                     /locus_tag="JN749_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7367347.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(33957..35030)
                     /locus_tag="JN749_000847"
     CDS             complement(33957..35030)
                     /locus_tag="JN749_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HAY7367348.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(35152..35313)
                     /locus_tag="JN749_000848"
     CDS             complement(35152..35313)
                     /locus_tag="JN749_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HAY7367349.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(35440..36045)
                     /gene="osmY"
                     /locus_tag="JN749_000849"
     CDS             complement(35440..36045)
                     /gene="osmY"
                     /locus_tag="JN749_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HAY7367350.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(36438..38027)
                     /gene="prfC"
                     /locus_tag="JN749_000850"
     CDS             complement(36438..38027)
                     /gene="prfC"
                     /locus_tag="JN749_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAY7367351.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(38118..38795)
                     /gene="yjjG"
                     /locus_tag="JN749_000851"
     CDS             complement(38118..38795)
                     /gene="yjjG"
                     /locus_tag="JN749_000851"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HAY7367352.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(38810..39256)
                     /gene="rimI"
                     /locus_tag="JN749_000852"
     CDS             complement(38810..39256)
                     /gene="rimI"
                     /locus_tag="JN749_000852"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAY7367353.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(39225..39638)
                     /gene="holD"
                     /locus_tag="JN749_000853"
     CDS             complement(39225..39638)
                     /gene="holD"
                     /locus_tag="JN749_000853"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HAY7367354.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            39741..40772
                     /gene="rsmC"
                     /locus_tag="JN749_000854"
     CDS             39741..40772
                     /gene="rsmC"
                     /locus_tag="JN749_000854"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HAY7367355.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVITQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT         9723 a        11257 c        10367 g         9698 t
ORIGIN      
        1 tggcctccgc atccggcagc acaagggatt gtccagatga ttggcatttt tatcgacacc
       61 ctggtcatct gtacggcaag cgccatgctg atattactgg cgggtaacgg cacaacctac
      121 atgccgctgg aaggtattca gcttatccag aaggcgatgc gggtgctaat gggttcctag
      181 ggtgctgaat ttgttaccct cgtggttatt ctgtttgcct tcagctccat cgttgccaac
      241 tacatttatg ccgaaaacaa tctcttcttt ttacgcctga acaaccctaa agcgatctgg
      301 tgtttgcgga tctgcacctt cgcaacggtc atcggcggca ccttgctaag ccttccgctg
      361 atgtggcaac tggcagatat cataatggcc tgcatggcta ttaccaattt gaccgccatt
      421 ttactgctct cgcctgtggt tcataccatt gccagtgatt atctacgcca gcgtaaactc
      481 ggcgtgcgcc cggtgtttga tccgttgcgt tatccggata tcggtcgcca gctttctccg
      541 gacgcgtggg atgatgtttc gcaggagtaa tcacaactat cgatcaactc attctctttt
      601 tttgctaaag tcggcataaa tttcctgcaa ggactggata tgctgattct tatttcacct
      661 gcgaaaacgc ttgattacca aagcccgttg accaccacgc gctatacgct gccggagctg
      721 ttagacaatt cccagcagtt gatccatgaa gcgcggaaac tgacgcctcc gcagattagc
      781 acgctgatgc gcatcagcga caaactggcg ggtatcaacg ccgctcgctt tcatgactgg
      841 cagccagatt tcacgccggc gaatgcccgc caggcgattc tggcgtttaa aggtgatgtc
      901 tacaccggct tgcaggccga aaccttcagc gaagacgatt tcgattttgc ccaacagcat
      961 ttgcgaatgc tttccggctt gtatggcgta ctgcgcccgc tcgatttaat gcagccttat
     1021 cgtctggaaa tggggatccg tcttgagaat gcccgaggga aagatctgta tcaattctgg
     1081 ggagatatca tcaccaacaa gctgaacgag gcgctcgcag cacaaggcga taatgtggtg
     1141 attaatctgg cgtcagatga gtattttaaa tcggtgaagc cgaagaaatt gaatgccgag
     1201 attatcaagc cggtgttcct cgatgagaag aacggcaaat ttaagatcat cagcttctac
     1261 gctaagaaag cacgcggtct gatgagtcgt ttcattattg aaaatcggct gaccaaacca
     1321 gagcaactga ctggttttaa tagcgaaggt tacttctttg atgaagattc ctccagcaat
     1381 ggcgaactgg tgtttaaacg ctacgagcag cgttaagttt gtagtcaatg ccggatgcgg
     1441 cgtgaacgcc ttatccggcc tacaaaagca tgcagattca atatattgca gaattaacgt
     1501 aggcctgata agacgcgcca gcgtcgcatc aggcgctgaa tgccggattg ttacccggca
     1561 tttgtcattt agcggtgatg tttgcctgga ccatgaccgc cgtgatgatc atgaggagct
     1621 ttcttatggt ggcgcggcgg tggcggcggt ccgtgtgggt gccagcgatt gcctcgccat
     1681 tcataatgtt gtttccacca gccgtggtcg cgccagtgac ctccatccca gtaatagcca
     1741 cgattatcac gatcgcctat ctgtaatttt actgacggga ctaacgtaat ttccgcagcc
     1801 tgtgctgcca tgggagcgac cagaaccagg gaaagtgcga gtacgataga ttgcatcttt
     1861 ttcacaggtt actcctttta tcattgcccg ttatgggtcg atatccggag attacgcgat
     1921 gagaacgacg gttgttattc tccgcaatcc taatctctaa gttaccgcat ttattgataa
     1981 tttctccttt ttccctgtcg tttttctcca taatttcttc ataaaaaagc cgggctgcat
     2041 aaaagcaaac ccggcctgat tgagataatg aatagatttt actgatgatt catcatcaat
     2101 ttacgcaacg cagaaaaatc ggcgggcaga ttatgtgaaa gcaagggtaa atcagcacgt
     2161 tctgccagct cttttggcag atccaacgtt tcaccgagaa tcgcttccac gctctcttta
     2221 aatttcgccg gatgcgcggt gccgaggaac aagccatatt cgcctggatt caactggtca
     2281 cgcagcgcac gatacgctac ggcagcgtgc ggctccgagg tgtagcccag ttcttttaac
     2341 tcacgcattg tctgttgcgt ggtttcatca tccacggctg cataacccag ctctttcagt
     2401 tgccagattt tgcggcggaa caactcttcc acacgcggcc agttgttcgg ctgactcacg
     2461 tccatcgcgt tggataacgt cgcctgagtc gctttgggtg accactgacc gtcgtgcagg
     2521 aaacgtggca cggtatcgtt cacgttggtc gcagcaataa aacgtttcac cggcagaccg
     2581 aatgacttcg ccagcagacc cgccgtcaaa tcgccgaagt ttccgcttgg caccgagaca
     2641 accagctggt tgcgcgcctc ctgcggcagc tgcgcaacag cttcaaagta gtagcaaatc
     2701 tgcgccagca aacggctgat gttaatcgag ttagccgagt ttaaccccag cgccactttc
     2761 agttcttcat catcaaacgc ctgcttcacc agcgcctgac aggcatcgaa atcgccgtcg
     2821 atggcaaccg tttcgatatt gccgcccaac gtacagaaca gtttttcttg cagtggactg
     2881 attttgcctc gtggatagag gataaccact ttcacattcg gcaaaccgta gaaagcatga
     2941 gccactgcag ctccggtatc accggaggtc gcggtcagaa tggtcactgg cttatcatcc
     3001 gcaatatggg tcagcatttg tgccataaag cgaccgccga aatctttaaa tgccagcgtt
     3061 ggcccgtgga acaattccag acaaccgaca tcgctttcaa cattggcgac cggagccggg
     3121 aaggcaaacg ccgcgcgcac gcgctcttcc aggatttcct gtgggatttc atcaccaata
     3181 aacgccgaga ggatcttcgc actgcgggtg acaaaatcca gcttcagcat ctcatcaatt
     3241 tcagtcaggc tgaattccgg caggtcgtgc ggaaaaaaca gcccctgatt tttgcccaac
     3301 ccctgggtta cggcttgcgc aaagctgacc tgctcgttgt gatctttcag attgtagagt
     3361 ttcatttagt tttccagtac tcgtgcgccc gccgtatcca gccggcaaat atgaacaaaa
     3421 ccttcctgat tttgcaggta gttcttaccc aaccagtcgg caacgcgctg ggcggtatcc
     3481 ggcttgtcac atagagcgaa caaggtcggg ccggagccgg agataccgct cgctaccgcg
     3541 ccgatttccg cgaccgcctg ccgcgcctgc cggaagccag gcagtaaccg ttcacggtag
     3601 ggttcagcga taacatcttt catcagcttc gcggcaagct caggctgacg ggaatagcag
     3661 gcgtgaatga agccagccag atgtcgcccg tgcgcaatgc aatcctggcg gcgatactgc
     3721 gccggtaaaa tagcccgggc ttctgccgtc gagactttaa ttcccggata cgccagcacc
     3781 cacagccact catcaaaccc tggcacttgc tggctgatga tgtcgttttc ttcgatcatc
     3841 aactgcatac caccaagaaa acacggtgcc acgttgtcgt aatgaatgct gccggagata
     3901 cgtccttcca gctcgcccat caaagccagc aaacgagtgt cattaagcgg cttgccgcag
     3961 tgttcattca tcgccatcag cgccgcgacc accgaacagg cgctggagcc taagcccgaa
     4021 ccgatcggca tattcttttc cagagtcatc gccactggaa tttgcttgcc aagctcctgg
     4081 caaaaacgct cccagcactg ataaacgata ttttcccgtg gttctgacgg cagcttatcg
     4141 gcaaagcgtc cgaggttgtt gagactgaat gtctctgccg cctcaaccgt gactacatct
     4201 ccgagcaatg caccatcaac gggtgccacc gccgccccga gcacatcaaa cccgacgctc
     4261 atattggcac tggaagccgg ggcataaact ttaaccatgt cagactccta acttccatga
     4321 gagggtacgt agcagatcgg caaagacacc ggcagcggta acgtcattgc ccgcaccata
     4381 tccgcgcagc accaacggca gcggctgata atagtggcta taaaaggcca gggcgttttc
     4441 gccatttttc actttgaaca gcggatcatt cccatccact tcggcaatct tcacgcggca
     4501 gacaccatct tcatcaatat tgccaacata gcgcaaaact tttccttcat cacgggcctt
     4561 cgccacgcgc gcggcaaaga gatcgtcgag ctgtgacaga ttcgccataa aagcggcaac
     4621 atcaccctcg gcgttaaact ctgcgggcag cacaggttca atttcaatat ccgccagctc
     4681 cagttcacgt cccgtttcac gagcgagaat caatagttta cgcgccacat ccataccaga
     4741 aagatcatct cgcgggtccg gttcggtata acccatttcc cgcgccagcg tggtcgcctc
     4801 ggagaaactc atgccttcgt ctaacttgcc gaagatataa gaaagcgaac ctgaaagaat
     4861 gccggagaac ttcaccaatt catcacctgc attgagcaga ttttgcaggt tctcaataac
     4921 cggtaatcca gccccaacgt tggtgtcata gaggaattta cgccgcgatt tttccgccgc
     4981 ataacgcaac tgatgatagt aatccatcga cgaggtgttg gcctttttgt tcggcgtgac
     5041 aacgtggaaa ccttcgcgca ggaagtcggc atattgatcc gccactgcct ggctggaggt
     5101 gcagtcaaca atgaccgggt tcagcagatg atattctttc acgaggcgaa ttaagcgccc
     5161 gagattaaac ggctctttgg cttgtgcaag ttcttcctgc cagttttcca gattaaggcc
     5221 atgcacacta gtgagcagag ccttcgagtt ggcaacgccg cagacacgta agtcgatatg
     5281 tttattcttc agccagcttt gctgacgctt cagttgctcc agcagcgcac cgccaacgcc
     5341 accgacgcca atcacaaaca cttcgataac ctgatcggta ttgaacagca tctgatgagt
     5401 aacgcgcacg ccagtggtcg catcatcgtt atttaccacg acagagattg agcgttcaga
     5461 agatccctga gcaatggcga caatgttgat attggcgcgg gccagcgcgg caaagaattt
     5521 cgccgagatc ccacgcaagg tgcgcatacc atcacctacc accgagataa tggccagccg
     5581 ttccgtcacc gccagcggct ccagtaagcc ttctttcagt tccaggtaga actcttcctg
     5641 cattgcccgt tcagctcgca cacagtcgct ttgtggaacg cagaaactga tactgtattc
     5701 ggaagatgat tgcgtaatca gcaccacgga aatacgggcg cgtgacattg cagcaaagac
     5761 gcgcgccgcc atgccaacca tccctttcat ccccgggccg gaaacgctga acattgccat
     5821 gttattcaga ttggaaatgc ccttgaccgg taattcgtct tcatcacggc tggcaccaat
     5881 gagcgtacct ggtgcttgag gatttccggt atttttaatc aggcaaggga tctggaactg
     5941 ggcgatgggg gtaatggtgc gggggtgaag aactttagcg ccgaagtagg aaagctccat
     6001 cgcttcctgg taggacatcg acttcaacaa cctcgcatcg ggcacctgac gcgggtcgca
     6061 ggtatagacc ccgtcaacgt ccgtccaaat ctcgcaacaa tcggcgcgta aacaggcagc
     6121 cagcaccgcc gcggagtagt cggaaccgtt acgtccaagc accaccagtt cgcctttttc
     6181 attaccggcg gtgaaacctg ccatcaacac catgtgatca gccggaatgc ggcttgccgc
     6241 aatacggcgg gtggactcag caatatcgac ggtagattcg aggtaatgcc ccactgccag
     6301 cagtttttcg accggatcga taacggtaac gttgtgacca cgcgcttcta acacgccggc
     6361 cataatggcg atcgacattt tctcgccacg gcaaatcagc gcagcgttga tgctatccgg
     6421 gcactgcccc aacaaactaa tgccatgcag gacatgtttt atttgggcaa attcctgatc
     6481 gacgaaagtt ttcaattgcg ccagcgggaa ccccggctgg gcggcggcga gtcccgtcaa
     6541 aagttcggca aaaatacgtt cggcatcgct gatattgggt aaagcatcct ggccgctaat
     6601 ggttttttca atcatcgcca ccaggtggtt ggtgattttg gcgggggcag agaggacggt
     6661 ggccacctgc ccctgcctgg cattgctttc cagaatatcg gcaacacgca gaaaacgttc
     6721 tgcatttgcc actgatgtac cgccgaactt caacactcgc atggttgtta cctcgttacc
     6781 tttggtcgaa aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt ttcctgtacg
     6841 cgtcagcccg caccgttacc tgtggtaatg gtgatggtgg tggtaatggt ggtgctaatg
     6901 cgtttcatgg atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat gcctatattg
     6961 gttaaagtat ttagtgacct aagtcaataa aattttaatt tactcacggc aggtaaccag
     7021 ttcagaagct gctatcagac actctttttt taatccacac agagacatat tgcccgttgc
     7081 agtcagaatg aaaagctgaa aaatacttac taaggcgttt tttatttggt gatatttttt
     7141 tcaatatcat gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa aagccccagg
     7201 cgttgctgta accagtcgac cagttttatg tcatctgcca ctgccagagt cgtcagcaat
     7261 gccatgactc gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt gtcacttttc
     7321 gccggttgtt gtattaatgt tgctaattga tagcaataga ccatcaccgc ctgccccaga
     7381 ttgagcgaag gataatccgc caccatcggc acaccagtaa gcacgtcagc caacgctaac
     7441 tcttcgttag tcagcccgga atcttcgcga ccaaacacca gcgcggcatg gctcatccat
     7501 gaagattttt cctctaacag cggcaccagt tctactggcg tggcgtagta atgatatttc
     7561 gcccgactac gcgcagtggt ggcgacagtg aaatcgacat cgtgtaacga ttcagccagt
     7621 gtcgggaaaa ctttaatatt atcaataata tcactagatc catgtgcgac ccagcgggtg
     7681 gctggctcca ggtgtgcctg actatcgaca atccgcagtt cgctaaaacc catcgttttc
     7741 attgcccgcg ccgctgcccc aatattttct gctctggcgg gtgcgaccag gataatcgtt
     7801 atacgcatat tgccactctt cttgaccaaa taaccgcgaa cagggtgatc actgtcaact
     7861 tattacgcgg tgcgaattta caaattctta acgtaagccg cagaaaaagc cctttactta
     7921 gcttaaaaaa ggctaaacta ttttctgact gcactaacgg tttaattgtt aaaaaatgct
     7981 acatatcctt ctgtttactt aggataattt tataaaaaat aaatctcgac aattggattc
     8041 accacgttta ttagttgtat gatgcaacta gttggattat taaaataatg tgacgaaagc
     8101 tagcatttag atacgatgat ttcatcaaac tgttaacgtg ctacaattga acttgatata
     8161 tgtcaacgaa gcgtagtttt attgggtgtc cggcccctct tagcctgtta tgttgctgtt
     8221 aaaatggtta ggatgacagc cgtttttgac actgtcgggt cctgagggaa agtacccacg
     8281 accaagctaa tgatgttgtt gacgttgatg gaaagtgcat caagaacgca attacgtact
     8341 ttagtcatgt tacgccgatc atgttaattt gcagcatgca tcaggcaggt cagggacttt
     8401 tgtacttcct gtttcgattt agttggcaat ttaggtagca aacatgcaga ccccgcacat
     8461 tcttatcgtt gaagacgagt tggtaacacg caacacgttg aaaagtattt tcgaagcgga
     8521 aggctatgat gttttcgaag cgacagatgg cgcggaaatg catcagatcc tctctgaata
     8581 tgacatcaac ctggtgatca tggatatcaa tctgccgggt aagaacggtc ttctgttagc
     8641 gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc gtgacaacga
     8701 agtcgataaa attctcggcc tcgaaatcgg tgcagatgac tacatcacca aaccgttcaa
     8761 cccgcgtgaa ctgacgattc gtgcacgcaa cctgctgtcc cgtaccatga atctgggtac
     8821 tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc aatggttggg aactggacat
     8881 caacagccgt tcgttgatcg gccctgatgg cgagcagtac aagctgccgc gcagcgagtt
     8941 ccgcgccatg cttcacttct gtgaaaaccc aggcaaaatt cagtctcgtg ctgaactgct
     9001 gaagaaaatg accggccgtg agctgaaacc gcacgaccgt actgtagacg tgacgatccg
     9061 ccgtattcgt aaacatttcg aatctacgcc ggatacgccg gaaatcatcg ccaccattca
     9121 cggtgaaggt tatcgcttct gcggtgatct ggaagattaa tcggctttac caccgtcaaa
     9181 aaaacggcgc tttttagcgc cgtttttatt tttcaacctt atttccagat acgtaattca
     9241 tcgtccgttg taacttcttt actggctttc attttcggca gtgaaaacgc ataccagtcg
     9301 atattacgag tcacaaacat catgccggcc agcgccacca ccagcacact ggttcccaac
     9361 aacagcgcgc tatcggcaga gttgagcagt ccccacatca ctccatccag caacaacagc
     9421 gcgagggtaa acaacatgct attgcgccag cctttcaata ccgcctgcaa ataaataccg
     9481 ttcattaacg ccccaatcag actggcgatt atccatgcca cggtaaaacc gatatgttca
     9541 gaaagcgcca gcaagagcaa ataaaacatc accaatgaaa gccccaccag caaatattgc
     9601 attgggtgta aacgttgcgc ggtgagcgtt tcaaaaacaa agaacgccat aaaagtcagt
     9661 gcaatcagca gaatggcgta cttagtcgcc cggtccgtta attggtattg atcggctggc
     9721 gtcgtcactg cgacgctaaa cgcggggaag ttttcccagc cggtatcatt gcctgaagca
     9781 aaacgctcac cgagattatt agcaaaccag ctgctttgcc attgcgcctg aaaacctgac
     9841 tcgctagctt cccgtttggc tggtagaaaa tcacctaaaa aactgggatg cggccagttg
     9901 ctggttaagg tcatttcgct attacgcccg gcaggcacca cagaaagatc gccggtaccg
     9961 cttaaattca gtgccatatt cagctgcagg ttctgcttcc gccagtcccc ttcgggtaaa
    10021 gggatatgca cgccctgccc gccttgctct aacccggtac cgggttcaat ggtcagtgcc
    10081 gttccgttaa cttcaggcgc tttcaccaca ccaataccac gcgcatcccc gacgctaatc
    10141 acaataaagg gcttgcctaa gatgatattt ggcgcgttga gctcgctaag acgcgaaaca
    10201 tcgaaatcgg cttttagcgt taaatcactg tgccagacct gaccggtata aatccctatc
    10261 ttgcgttctt ccacgttctg attgccatca accatcaatg actcaggtaa ccaaaaatgg
    10321 ataaaacttc gtttccgctc cacggtttta tcctcttcct gcaccgtata aagctcggtc
    10381 acaggaatag cgataagcgg cccaacgagt ttttgcggcc cgctggtact ttggcgaatc
    10441 gcatcttcca catcgctacg gtaatcggca cgttcgacaa tcacttgccg aatcagcatt
    10501 atcggaatca acaacagcaa tactgcacca aacaaggtgg tcattttcca gaacaggggg
    10561 gatttcaaca tagtcttctc ctttgcaatg gcagcaggat acgaaggcta tgtgggaaga
    10621 atttgaagct atgtgaagtt acggtgaagt cgtagcgagg ccagcacgcc accttcctgc
    10681 acgttgcgca gcgtgacttc gccgttaaac aaacgggcga cctcactgac gaacgccaac
    10741 cccagaccgc tgcttttttg cccatttgca cgcggcaaag agtaaaagcg ttcaaaaata
    10801 cgtgacagcg cgtagtcagg aataccacta ccggtatcca gcaccttaag cgtgacgtgt
    10861 tcctgatcca cttcggcgct tagcgttatg caaccgctct cgggagtaaa atcgatggtg
    10921 ttatccagta aattccccag cgcctgatcc agtaacgccg gttcagcagc aacattaacc
    10981 tcggtgggca taacatgcaa agtgatgttt ttttctgcca actgcacggt gcgcgcttcg
    11041 ctgacgcggc gaaataatgc cgccacatca acagcagtca gaacgacttc ctgacgattc
    11101 tccagtcttg cctggcgtag taacgtttcc accagcgcct gcattcgcgc attttgtgtc
    11161 agaatgttgt cagtaaaacg agccactact tcaggcggcg gaccttcgcg taaaatttcc
    11221 gccacgccac gaatcgccgc cagtgggctt tttagctcat gggttaacgc ataaacatac
    11281 tgctcaatat agtttttccc ttccagcttc acgcgcatac tttccagcgc ctgcgcgagt
    11341 ttacgcagct cgctactacc gagatcggga agaggaacgg gcttattgtc agtgacggaa
    11401 tcagcatagc gagtgagccg ggcaatggag cggttgatcc accaaaccat gcctgcgcca
    11461 atcaccagtg caatccccaa caaaatggcg ctggcccata aaattcgccg ctcgctgcgt
    11521 ttaatgaccg gagccatcgc cgcgttcggt ttgcctacgc tcaaaacgcc aataagccgc
    11581 gagccgccca taatcggcgc ggcaacatac atcaccgaac tttcgggatc ggcaggattt
    11641 tgcaacgtgc tgcgcgcacc atactgacca cgcaacgtta gccagacgtc attccagcgc
    11701 gaataatcct gtccaacggc tttatttgcc gaatcgaaca atactttgcc atgcgcatcg
    11761 gtcatataga catgatattc attgcgcact ttgttaatgc caccaatatt ggcgcgaaac
    11821 gggcgatgtt gtagctgatt aaacgcctgc gccagttgcc catgcgttgg gtccccagag
    11881 agcaaatcgg gacgcgccag ctccgcaagt aacgttgccg tgtcgatcaa cgtcccctcc
    11941 gttgctcttc gcacgcccgg tttaacttct ttgacaaaaa tagccagtac gaaccaggcc
    12001 gccaccgcca ccagtaaaaa atagcccagc agcaaccgca tgccgatacg cattacaagc
    12061 ccctcaggct atatcccatg ccgcgatgag tattaatcgg tgaaagatcg gggttgatgg
    12121 cgcgcagctt ggcacgcagc gttttaatgt ggatatcgac ggtgcgatcg taggtgtcct
    12181 gcgcatcttc ccacacgcta tccattagtt gctggcggga ccagacgcgg cctagcgact
    12241 tgagtaacgt cttcagcaat aaaaactcat accgcgtcag cgttaatggc gtgtcaaacc
    12301 agctgatctg cgccgcgggt tcattcaatt caaaatgacc aatacggatg acgggagacg
    12361 gcgtcgagaa cttcttcacc cgacgcagta aggtgcgcac cctggcgcac acttcgcggg
    12421 gtgaaaacgg tttagccaca tagtcgtcag cgccaatttc cagcccaagc aggcgatcga
    12481 cctcttcact tcgggccgtc aggaacagta caggtaacgc cggatggagc gccagtaact
    12541 ggcggcacaa ttcaaagcca ctgatatcag gcaaaccaac gtcgagaatc atgacgtcgg
    12601 gaacctgctg gcgagcttta tccagcaccg gcaaacctcg ctcaaagacc tcgacggcaa
    12661 aaccttcctg ctgcaacatg tagaccagcg tgtcggctat cccttgctca tcttccacta
    12721 accagaccgt ttcccgttgc ataaatcgcc tctgttattg ccgccacggc atgacaggaa
    12781 ccgcgctaat cgcgtttttc ggcgaacctt ctacaacttt gtcggagtaa gccagataag
    12841 cgagcgcgtt gcgtttggcg tcataaaagc gcacgacctg taacgactta aagaccaggg
    12901 acgtgcgttt tttgaatact acctcgccct gagctttgcc gtttttaata cgatccgaca
    12961 gttcaatcgg cccgacttgc tgacaagaaa tggccgcatc ggaggtatct tctgccaggc
    13021 ccaatccccc tttaatacca ccggttttcg cccggctcac ataacaggtg acatttttca
    13081 catcgggatc gtcaaaggct tccacaacaa ttttgtgatc cgggccgatc attttaaata
    13141 cggtgtcgac cgaaccaatc tcttctgcat gagccaatgg ccccagcatt atcaggctta
    13201 aagacaggat caaatgcttg tatttcatat tgttaccatt tttatctatt acacgagcaa
    13261 ttagttcgtc acggtaaata acatattgat taagaacaaa atctcaattc ttttatttcc
    13321 gtcaggccct aaaacatact ctactaagaa aaaaacactg aatgctaaaa cagcaaaaaa
    13381 tgctattatc caattacctg atgtcaggtg ctcgttgttg aaaggatgag gatattttat
    13441 ggatcaggcc ggcattattc gcgacctttt aatctggctg gaaggtcatc tggatcagcc
    13501 cctgtcgctc gacaatgtag cggcgaaagc aggttattcc aagtggcact tacagagaat
    13561 gtttaaagat gtcactggcc atgctattgg cgcgtatatt cgtgctcgtc gtttgtcgaa
    13621 atcggcggtc gcactacgcc tgactgcgcg tccgattctg gacatcgcgc tgcaataccg
    13681 cttcgactct caacagacat ttacccgcgc attcaagaag cagtttgccc agactcctgc
    13741 actttaccgc cgttctcctg aatggagcgc ctttggtatt cgcccgccgc tacgtctggg
    13801 taaattcaca atgccagagc acaaatttgt caccctggaa gatacgccgc tgattggtgt
    13861 tacccagagc tactcctgtt cgctggagca aatctctgat ttccgccatg aaatgcgtta
    13921 tcagttctgg cacgattttc tcggcaacgc gccgaccatt ccgccggtgc tctacggcct
    13981 gaatgaaacg cgtccgagtc aggataaaga cgacgaacaa gaggtattct ataccaccgc
    14041 gttagcccag gatcaggcag atggctatgt actgacgggg catccggtga tgctgcaggg
    14101 cggcgaatat gtgatgttta cctatgaagg tctgggaacc ggcgtgcagg agtttatcct
    14161 gacggtatac ggaacgtgca tgccaatgct caatctgacg cgccgtaaag gtcaggatat
    14221 tgagcgatac tacccggcag aagacgccaa agcgggagat cgcccaatta atctgcgctg
    14281 tgaactgctg attcctatcc gccgttaacg ctgcagctca tctaatgcag gggcgtccag
    14341 atgcgagatg tcccctgcgg tttcaacgac ccagccagac gccagccaca ggctttcctg
    14401 ataatccacg cgcgaaatag aacagttacg cagacgtaag cggcgctctg cccatgctgg
    14461 taatccgaga atcgtactca ccagacatcc cagtgcaata ccatggctca ccagcagcgg
    14521 tcggcttccc tgcggtaagt cccggcaaga ttccagtgcc gcattgacgc gatcgctgag
    14581 ctcctgcatt gactcgcctt caggaatacg cccgtcaacg gtgccattga ccagctgccg
    14641 acgccagttc tcttcttctt cggtcagaga atcgatatgt cttttttcca gcacacccat
    14701 gtttaattca cgcaggcgag aatcaaagat gatgtcacag ccgcaggcct gggcgatgat
    14761 ttccgccgta cgccgggtgc gtcctaaatc gctactgata atatgcgtaa tgccaagctc
    14821 tttggcacgg gttgccacct gcatcgcctg ttgctcacct ttggcggtca gcgggctgtc
    14881 agactggccc tgaatacgtc gctcggcgtt ccactgcgtt tcaccgtggc ggactaggta
    14941 tacctgtaac atgctttttt tccgtatact gctgtaacgt taattttctg agtaatgctg
    15001 attatgcacc aagttgtctg tgcgaccacc aatcccgcta aaattcaggc cattctgcag
    15061 gcatttcacg agatcttcgg cgaaggatcc tgccatattg catccgttgc cgtcgagagc
    15121 ggtgtaccgg aacaaccctt tggcagtgag gaaacgcgcg ctggcgcacg aaatcgggta
    15181 gcaaatgccc gccgtttact tccagaggct gatttttggg tggcgattga agctggcatt
    15241 gatggcgaca gcactttcag ttgggtggtg attgaaaacg ccagccagcg cggcgaggcg
    15301 cgttcggcaa ctttgccgtt acctgcggtg attctggaaa aagttcgtga aggcgaggcg
    15361 cttggtcccg tgatgtcgcg ttataccggt attgatgaga ttggccgtaa ggaaggggcg
    15421 atcggcgtgt ttaccgccgg gaaactcact cgcgccagcg tctatcacca ggcggtgatc
    15481 ctggcgctga gtccgtttca caatgccgtg tatcaggcat tgtaggacgg ataaggcgtt
    15541 cacgccgcat ccgacattcg gtgcacgatg cctgatgcga cgctgccgcg tcttatcagg
    15601 cctacaaaat caatcgcttt tcagcaacac ctcttccagc cactgacgca gctccacggg
    15661 cgcggctttc aggctgttag atccacgcgt aatcgtcgcg atgcccgcgc cgagttcatt
    15721 ttttaactca cgctgactca tttcgccacg caacagctct tcgacaatac gcacgcgagt
    15781 ccccaacgct tcgcgctcat ctggcgtcag catcaggttt aacaacggta aatggagatc
    15841 gttttggtag gcattcttaa gcaggtcgac aaaacgtaac cactcctggt gacgctgttc
    15901 tgccatcgct gctgaatagg gtgattgttg ggccataata tgtcgccatt gttagcgggg
    15961 gaagcaaaat gactcccccg gttgtactcg ctaaagagta cgatagcata tcataaacgt
    16021 gcggatcagt aacgacgtcc ccattccgtg gcgctcatca acgtcggttt atcccccatg
    16081 aaatagcggt agtaagcatc ataagccagc acgttcttca cataaccgcg cgtctcggag
    16141 aacggaatac tctcgacaaa tgccacagcg tcgatacgcc cggcgctgtt gccaagccag
    16201 gttcgcaccc gtccaggtcc ggcgttataa gctgctgagg agaaaatacg gttattgcca
    16261 aactgctgat aaacatattg caggtaactg gtgccaatgt tgatgttcgt ttccggatcc
    16321 agcaattgcc cagggctgct ataaccagga atagagaaca tcttcaccgt atgggtcgct
    16381 gtaccaggca taatctgcat caggccgctg gcccctaccg gtgatttcac tttcggattc
    16441 caggcgctct cctgacgggc aatcgccatc gcatagcttt gcgggatctc tttcccactg
    16501 gtgtaacgtt tgaaaagatc gttgtaagcc agcgggaatc gctcttccag atgatcccaa
    16561 agcttcccgg cgatcgttgc ctgaacgcta agatcccacc attggttatt gaaagcatac
    16621 cgagccagtt gagcctgctc tgtttttgac ttgctcttca ccagattggc ccactcgcta
    16681 cgcgcggtat tatcgagatt ccagtacatc aactcgcgca cgcgcgccat ctccggcccc
    16741 tgagtcaggg tgctgtcaac attctgcggc gctttatcaa tcttcagctc atactcttca
    16801 ccgatgcgtt gtgctgcaac catcgggtag aaaccacgct gttgcatgag ttgatgcaaa
    16861 atctctttcg cttcagcttc acgtccgcgt tccagcaata aatccgcctg ccagtaacgc
    16921 cattcatctt tctcttttgc ttccatcggc agacgcgcca gccaggtatt caggccgcga
    16981 cgatcgccgg tgccaagcgc cattcgcaca cggcgttcaa tcaacgacgt ggattgcgag
    17041 cgcataatgg cgtcatcgcg ccatttcgcc tgctcatcgg tgacatcatt gcccatcaaa
    17101 cgccaggcga cgatatcgcg cagctcctga atctcatctt cattaagctg ctgcgcctgg
    17161 gcaagcgacg ggatcatcag ccgtgcattt tcggcatcct gccgcgccac actggcaaac
    17221 gccaccgccg ccatttgacg ggtaaaatcg gtcgcgccgg ttgtacgcgc gaaggtcagt
    17281 accgtattag ggttgttcgc cagtgaaatg attgccgagg cgatagtctg gtaatcggca
    17341 ggcatctgcc ctgccagcac tgttaccagg cctgtgttac ccgctttcat cgccagacgg
    17401 atacgctcta aatacgccag cggatcttgt ttacctgacg cacgccagac gctaaataac
    17461 ttgtcacagg cgttaggctg gctcttgccg gttagccaca gctctttcgc cccttgccag
    17521 gcttcttcac tctgcccggt gttccatttc gcatagtagt aattacattg cgcttcggta
    17581 gttccgggct tttccgggct aaaggctaac aagccacgcc agtcttcacg ccgcgccagt
    17641 tcattgacga aacgagattg cagcgtgcga gcgggaggaa gcgtggggtt agcgcgaaca
    17701 aagttagtga ccgtcaccgc cggttgattc atcaggtcat cggtgatctg gcggtattcc
    17761 aggtagggat aaagcggata atccttcagt ccaggcatca tttgttcgac cacatccatc
    17821 tgtcgattat cccaggcctg cttgatttgc gcgtaacggc tacgctgctc atccagtgag
    17881 tcggctcgcg ccacgctgct gaccgtcagc agacagacac cggcagccaa cagccgccag
    17941 gtaacttgtt tggctttttc cacaagcgca tcctctaagt gtaaatacat caatgcagcg
    18001 tcataccgcg taatagattt atgctaacca gtcattgccg tttacgccac gttacggaca
    18061 cttttttact tttactgcga aggtgatcga ccaactggga ttaggcgggg aaacaggcta
    18121 cacttcgcct ttgaaaactc atcatcacga taaacataga ggcgaagtcc aacgtggctc
    18181 aattcgttta taccatgcat cgtgtcggca aagttgttcc gccgaaacgt catattttga
    18241 aaaacatctc tctgagtttc ttccctgggg caaaaattgg tgtcctgggt ctgaacggcg
    18301 cgggtaagtc caccctgctg cgcattatgg cgggcattga taaagacatc gaaggtgaag
    18361 cgcgtccgca gccagacatc aagattggtt atctgccgca ggaaccgcag ctgaacccgg
    18421 aacacaccgt gcgtgagtcc attgaagaag cggtttcaga agtggttaac gccctgaaac
    18481 gcctggatga agtgtatgcg ctgtacgccg atccggatgc cgattttgac aagctggccg
    18541 ctgaacaagg ccgtctggaa gagatcattc aggctcacga cggtcataac ctgaacgtac
    18601 agctggagcg tgcggcggat gcgctgcgtc tgccagattg ggacgcgaaa atcgctaacc
    18661 tctccggtgg tgagcgtcgt cgcgtggcgt tgtgccgcct gctgctggaa aaaccagaca
    18721 tgctgctgct cgacgaaccg accaaccacc tggatgccga atccgtggcg tggctggaac
    18781 gcttcctgca cgacttcgaa ggcaccgtgg tggcgattac tcacgaccgt tacttcctcg
    18841 ataacgttgc aggctggatc ctcgaactgg accgcggtga aggtattccg tgggaaggta
    18901 actactcctc ctggctggag cagaaagatc agcgtctggc gcaggaagct tcacaagaag
    18961 cggcgcgtcg taagtcgatc gagaaagagc tggagtgggt acgtcaggga actaaaggcc
    19021 gccagtcgaa aggtaaagca cgtctggcac gctttgaaga gctgaacagc accgaatacc
    19081 agaaacgtaa cgaaaccaac gaactgttta ttccacctgg accgcgtctg ggcgataaag
    19141 tgctggaagt cagcaacctg cgtaaatcct acggcgatcg cctgctgatt gatgacctga
    19201 gcttctcgat cccgaaaggg gcgatcgtcg ggatcatcgg tccgaacggc gcgggtaaat
    19261 cgaccctgtt ccgtatgatc tccggtcagg aacagccgga cagcggcacc atcactttgg
    19321 gtgaaacggt gaaactggca tcggttgatc agttccgtga ctcaatggat aacagcaaaa
    19381 ccgtttggga agaagtttcc ggcgggctgg atattatgaa gatcggcaac accgagatgc
    19441 caagccgcgc ctacgttggc cgctttaact ttaaaggggt tgatcagggt aaacgtgttg
    19501 gcgaactttc cggtggtgag cgcggtcgtc tgcatctggc gaagctgctg caggttggcg
    19561 gcaacatgct gctgctcgac gaaccaacca acgacctgga tatcgaaacc ctgcgcgcgc
    19621 tggaaaacgc cctgctggag ttcccgggct gtgcgatggt tatctcgcac gaccgttggt
    19681 tcctcgaccg tatcgccacg cacatcctgg actaccagga tgaaggtaaa gttgagttct
    19741 tcgaaggtaa ctttactgag tacgaagagt acaaaaaacg cacgctgggc gcagacgcgc
    19801 tggagccgaa gcgtatcaag tacaagcgta ttgcgaagta atgcgtaaaa tgccggatgc
    19861 ggcgcgaacg ccttatccgg cctacaaaac cagcaatttc aatacgttgt aaaactgtag
    19921 gcctgataag acgcggtaag cgtcgcatca ggcattgagc accgaatgcc ggatgcggcg
    19981 tgaacacctt ttccggtcta caacagcctt agcatcctca atttcgtggc gggtcacccg
    20041 tgcaggtttt ttcaaacctt gtttaatctc gacagcctct tccagagagg ttttcaactc
    20101 atcaaaaaaa ctcattttat acctcgcctt tgagcagttt cgtcacggtt atccttgctc
    20161 ccccatcatc tcccgcacca gctccacgca gcgcaggaaa cggctgtcgt aatcgtcctc
    20221 ttcaacccgc acgaattcga tattgttctc ttccagcatc tccaccagca agttctggaa
    20281 ctctttgcga tccaccgaac tgccgaggct gcgtaaacca tccgccaccc acggcgtgtt
    20341 gttctccagc aggatcacca gatcgaaacg gtattcatca atcagcgcct gcacgaacgg
    20401 atgctcacgc ccttcgtact ttttgcagaa cgcctgggta gtgacaaaat cggtgtcaat
    20461 aaacgccact ttattggcgt atttcactgc aaaatcaatg tattgtgcgt ggcccaacgc
    20521 gattttatcg tagtcggaat actgcaatgc gatctcatca ccgccgaggt gtgaaaagac
    20581 ataatcgcgg ccatattccc acgcactggt ggtgttgaag atattggcaa gtttgtttac
    20641 cagggtggat ttaccgctcg actcgccgcc aaggatcgcc acggtacgca caaaaaacgg
    20701 cttcacttcg gtaggaatat attcccagta gcggaacggg ttttcgcgga tctgcgcacc
    20761 gctgatactc ataaaggtac gtttcggatc gaccagcacc gtctcgatcc ccagatgttc
    20821 catatactgt ggcgcatcgg cttcttccga ggtgtagatc aggtccggct ggatcccctt
    20881 ttcagccata aactttttga tgccgttgct ccacacatcc cagccatgcg gatacggctc
    20941 catgccctct tcgttgaaag catgaatacg gatatttttc tgatatttaa aggtttgcaa
    21001 tagccagcgc agacgatccg gcaccgtggg ctgctgcgac atggcgctgt cttcaaacaa
    21061 cgcgcggtcg cgggtatcgt caaaacccat aatgatatgc agctcgtcaa cctggctaca
    21121 ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg tagaacttac cgaatacgac
    21181 accaatggtt ttcttctgcc ggggaaattc aagccccaaa aaacggtgca atgcctccag
    21241 cttttgcgcg ctggggcttt taattttggc attcagtaac tggcttaaat accctttggt
    21301 cataccgctg gcatcagcta cctgctgtag cgtgcagccc tgttgcttga tggcagtttt
    21361 caggtaatca aatgacgaca tatctccctc cgtatctctc attataagtc gtcgaacacg
    21421 ctaagcgcgt cggagagttt tttaacgcca aaaatctgca tcccttccgg cgcttttttc
    21481 ggtacgttag ccgccggaac aatcgcccgg cgaaaaccgt gtttcgccgc ttcagaaatc
    21541 cgctcctggc cactgggcac cgggcggatc tcccctgcca gcccgacttc accaaacacc
    21601 accagatcct gtggcagcgg tctgtcacgc aggctggaaa ccatcgccag cagtaacgct
    21661 aagtcggcgc tggtttcggt taccttcacg ccgccgacca cgttcacaaa cacatcctga
    21721 tcggccattt gcagaccacc gtgacggtgc aagacagcca gcaggattgc cagacggttt
    21781 tgttccagcc ccactgccac gcggcgcgga ttcgccatta tcgagtgatc gaccagcgcc
    21841 tgaatctcca ccagcaacgg acgcgttcct tcccacacca ccatcacgga gctgccggag
    21901 gtcacttcat ctccgcgact taagaaaatt gccgaagggt tgctgacttc acgcagcccc
    21961 tgttcggtca tcgcgaagac gcccagctca ttcaccgcgc cgaagcggtt tttatggctg
    22021 cgtaaggtgc gaaaacgaga gtcggcatcg ccatccagaa gcaccgaaca gtcgatacag
    22081 tgttccagca ctttcgggcc agccagcgaa ccatctttgg ttacgtgtcc aaccatgaca
    22141 atcgccacac cgcgcgtttt ggcgaaacgc gtcagataag ccgccgtttc acgcacctgc
    22201 gccacgctac caggcgatga ttgtacatcc gccatatgca tcacctggat cgagtcaatt
    22261 accatcagct tcggttgctc ttcttcggca atcaggcaga tctgctcgat gctggtttcc
    22321 gacaacatat tgagattgtc agtcggcagg ccaaggcgat gagcgcgcat tgccacctgt
    22381 tgcagcgact cttcgccggt gacatacagc gttttcatct gctgggccag tttgcacagc
    22441 gtttgcagca acagcgtgga tttccccgca ccagggttac cgccaatcag aatggcactt
    22501 cctggcacca cgccgccgcc tagtacgcgg tcgaactctt taaatccggt ggaaaaacgc
    22561 ggcagctctt caaggctgat atcggagagt ttctggactt ttgccacccc ggcgctaccg
    22621 gcatagccgc tgagacgctc gttacgcgcc accgttggcg acgcagcaag acgcacctcg
    22681 gtgatggtgt tccaggcatg acaggcactg cactgcccct gccagcgcgg ataatcggcc
    22741 ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg ccacggtgta cctcgttaat
    22801 gctgtgccgc ccgcaggatg acgggcaggc aattacttct gattcaggct gccagagagg
    22861 atgcagaata cccccatcag gtcagcgtga cggatggtga cttccgcctt ttcattcact
    22921 tttggcttgg catggtaggc aatccccagc cctgctgctt tgatcatcgg caggtcatta
    22981 gctccatcgc caatcgccac ggtctgcgcc agcgggattt catactcctg cgcgaggcga
    23041 gtcagagttt tcgctttgta ctgtgcgtct acgatgtcgc cgatcacatt gccggtaaat
    23101 ttaccgtcca tgatctccag ttcattggct accacggctg tcaggcgcag cttgtcgcgc
    23161 aggtattcag caaagaaagt aaagccgccg gaggcaatcg ccactttcca gcccagcgtt
    23221 tccagcttga gcaccagctg cgttaagcct ggcatcagcg gcagattttc acgcacctgt
    23281 tgcagaatat tggcgtcagc gcctttcagc gtcgccacgc ggctgcgcag gctggcggta
    23341 aaatcgagtt cgccgcgcat cgcccgttcg gttacctccg ctaccatctc gcccgttccg
    23401 gccagcttgg caatttcatc aatacattca atctggatgg cggtggaatc catgtccatc
    23461 accagcaaac ccggcgtgcg caggtgcggg attttcccca gcggggcgac atccagctgc
    23521 gcttcgtggg ccaggcgtgt agcccgtgcg gtgagtgaac ctgccagacg aatcacctga
    23581 taatcttcca cgcaccaggc ggcaacaatc accatcgccg cacccagttt gctctggtat
    23641 tgggtcagac gctgtttatc cagcccacga ccatacagca gccagccgct acgagctgcg
    23701 tggtaatcca gtggcatcac ttcatcacca cttaatgaaa gaggcagacc cggccataaa
    23761 gagacatctt caggcaggtc gcaccaggta atgttaggca ttaaggctcc tgtaaaatcg
    23821 ttcgaagcag ggaaaataac gcatgaggct accttgtatc cattgcttct ggcaacatta
    23881 agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa actgaaattc cggctacatc
    23941 gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc actgatgcag ggagcgtcat
    24001 ggtttagtca aaaccaccag cgacagcgta atccacagct ggaagaactg gcccgcaccc
    24061 tggcgcgtca ggtgacgctg aacgttgcac cgctgatgcg taccgactca ccggatgaaa
    24121 aacgcattca ggcgatcctc gatcagttaa cggatgaaag ccgtatcctc gacgcgggtg
    24181 tgtatgacga acaaggcgat cttatcgcac gttctggcga aagcgtcgaa gtgcgcgatc
    24241 ggctggcgct cgacggtaaa aaagcaggcg gctattttaa ccagcagatt gtcgagccaa
    24301 ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac actcgacacc catacgctcg
    24361 ccaccgaagc ccaacaggtg gataacacca ctaacatttt acgcctgatg ttgctgctct
    24421 cactggcaat cggcgtagtg ctgacccgca cgctgctaca gggtaaacgc acccgctggc
    24481 agcaatcgcc cttcctgtta accgccagca aaccggtgcc ggaagaggaa gaaagcgaga
    24541 aaaaagagtg acccattact acaagaaagg aaatcgttat gtccacatta cgcctgctca
    24601 tctctgactc ttacgacccg tggtttaacc tggcggtgga agagtgtatt tttcgccaaa
    24661 tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc cgacacggta gtaattggtc
    24721 gcgcgcagaa cccgtggaaa gagtgtaata cccggcggat ggaagaagat aacgtccgcc
    24781 tggcgcggcg cagtagcggt ggcggcgcgg tgttccacga tctcggcaat acctgcttta
    24841 cctttatggc tggcaagccg gagtacgata aaactatctc cacgtcgatt gtgctcaatg
    24901 cgctgaacgc gctcggcgtc agcgccgaag cgtccggacg taacgatctg gtggtgaaaa
    24961 ccgtcgaagg cgaccgcaaa gtctcaggct cggcctatcg cgaaaccaaa gatcgcggct
    25021 tccaccacgg caccttgcta ctcaatgccg acctcagccg cctggcaaac tatctcaatc
    25081 cggataaaaa gaaactggcg gcgaaaggca ttacttcagt gcgttcccgc gtgaccaacc
    25141 tcaccgagct gctgccgggg ctcacccatg agcaggtttg cgaggccata accgaggcct
    25201 ttttcgccca ttatggcgag cacgtggaag cggaaatcat ctccccggac aaaacgccag
    25261 acttgccaaa cttcgccgaa acctttgccc gtcagagtag ctgagagtgg aacttcggtc
    25321 aggctccggc attctcgcat ctgctggatg aacgctttag ctggggcggc gtggaactgc
    25381 atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt atttaccgac agcctcaacc
    25441 ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg cctgtaccgc gcggatatgc
    25501 tgcaacaaga gtgcgaagcg ctgttggttg acttcccgga ccaggaaaaa gagctacggg
    25561 agttgtcgac gtggatagcg ggggcggtaa ggtaactcgc tgattaaaat atccaaggaa
    25621 ggaaatatga tcgatctgga aaaccaggaa cgggagatta tcaatttaat gttcagccag
    25681 cgtatttcct ggctagccgc cgtgcgtatc aggcataaat tatcgcttgc tgaagtaagt
    25741 aaaatgttag gtatctcgat taattctcta aaacagatcg aaaagacgga acggcttagc
    25801 tcaaacatca aaagcaaaat ggcagaaatt tatggttgtc caccagagtt gctgatatgc
    25861 ccttcctgga tgacggctga acataaatga atagattcgt gaatagatac ttgaatgtat
    25921 tttattttct atccatatga aaaataatag taaatctata aatcagttgc tattgatgaa
    25981 tagatactaa aaagccggag cagtccccca ctccggctta acacgacaat cgcctttcag
    26041 cgaaacacaa ttactcttta tcgcccagca gaacggattc cagtgcgatt ttgatcatgt
    26101 cgttgaaggt ggtctgacgc tcagcggcag tggtctgctc gtgagtgcgg atgtggtcag
    26161 acacggtgca gatggtcagg gctttcgcgc caaactctgc agccacgccg tagataccag
    26221 ccgcttccat ttccacgccg aggatgccgt atttttccat cacgtcgaac atttcgccgt
    26281 ccggagagta gaacaggtca gcggagaaca ggttacccac gcgagcatca acgcccagtg
    26341 ctttagctgc atctactgcg ttacgcacca tgtcaaagtc agcgatagcg gcaaagtcat
    26401 ggtctttaaa acggatgcgg ttaactttgg aatcggtgca ggcccccata ccgataacga
    26461 cgtcgcgcag ttttacgtgc ggcagaactg cgccacagga accaacgcgg ataattttct
    26521 tcacgccgaa atcggtgatc agttctttgg tgtagatgga gcaggacggg atacccatac
    26581 cgtgacccat tacggaaatt ttgcggcctt tgtaagtacc agtgaagccc agcataccgc
    26641 gaacgttgtt cacttcacgg gcatcttcaa ggaaagtttc agcaatatac ttcgcacgca
    26701 gcgggtcgcc tggcatcaaa actacgtcag cgaaatcgcc catttctgca ttaatgtgtg
    26761 gggtagccat tgttttatcc tttttgtgac ataacaaagg cggagtcaac gctccgccca
    26821 aatccatcag aacatggctt tgccatattc catatcagaa gtaccaaaat attttgccag
    26881 agtctggccg atatccgcga aggtttcacg gtgacccagt gagcccggtt ttactttcgg
    26941 gccatatacc agtaccggaa tgtgttcacg cgtgtggtca gtaccggtcc aggtcggatc
    27001 gcaaccgtgg tcagcggtga ggatcaggat gtcgtcatcg cgcagcagag acatcagctc
    27061 cggcagacgg cggtcgaaca gttccagacc cgcagcataa ccggcgacgt cgcgacggtg
    27121 gccccaggaa gagtcgaagt caacgaagtt ggtgaagacg atggtgttat cacccgcttc
    27181 tttcatctct ttgatggtgg cgtcaaacag cgcgtccaag ccagtcgctt tcactttttt
    27241 ggtgataccg cagttggcgt agatgtctgc aattttaccg acagaaacca cctggccgtg
    27301 tttttcatca accagtttct gcagcacggt cggtgctggc ggctcaacag ccaggtcgtg
    27361 acggttaccg gtacgctgga agttaccagc tttgtcaccg ataaacggac gagcgataac
    27421 acgaccgata ttgtagccgc cgttggtcag ctcttcacgg gcgatttcgc acagttcgta
    27481 gagtttatcc agaccgaaag tttcttcatg gcaggcaatc tggaacacgg agtcagcgga
    27541 ggtatagaaa atcggcttgc cggttttcat gtgctcttcg cccagttgat ccagaatgac
    27601 cgtaccggaa gagtggcagt taccgaggta acccggcaga ttagcgcgtt cgaccagttt
    27661 atccagcagc tcttgcggga agctgttttc gtgatcggag aaatatcccc actcaaacag
    27721 aaccgggaca ccggcgattt cccagtgacc agacggggta tctttaccgg atgacatttc
    27781 gtgcgcccat gcgtacgcgc cgataacttc agcgttgccg tccattcccg ccggaatgaa
    27841 accggtagaa ccttcgtgtg ctttcgccag ccccagacgg atcagatttg gcagattgag
    27901 cggcccttta cgaccattat cagcttcgcc tttggcacaa gcttctgcga tatgacccag
    27961 ggtgtcagcc ccgacgtcac caaagcgttc tgcatcttct gtagcgccga tgccgaatga
    28021 gtccagcacc ataataaatg cacgtttcat atgttctccg tactcagtgc ttcaaattta
    28081 aatgatcaga tcagtatacc gttattcgct gatacggcga tagacagttg gtgtgctttc
    28141 cggtgcgtta tcggcaagtt taattgccgc tttcaccgct ttcgccgctt cctgccagct
    28201 gttttcgtct ttcgcgagga taaccgccag cggacgctga ccgtctacct ggtcgcccag
    28261 acgcgccata tcagtaaagc cgacgctgta atcgatggtg tcagatgcct gacgacgacc
    28321 gccgcccatt gcaaccactg ccatccccag cgcgcgggta tccatttcac tgacaaaacc
    28381 ttcggtatca gcatagactg ctttcgtcag catcgctgtc ggcagatact tcgcgtagtt
    28441 ctcaacgaag tcggtcgggc ctttttgtgc cgctaccata cgaccaaaga cttctgccgc
    28501 tttaccgttg tccagcaccg cctgcaattt cgcgcgcgct tcggcgtcat ctttcgccag
    28561 tttgccggag atcagcatct ccacgcacag caccatcgtg acatcaaaca gacgcgggtt
    28621 acgatactca cccgtcagga actgcaccgc ttcacgaact tcaaccgcgt tacctgcact
    28681 ggaggccagt acctgattca tgtcggtgag cagcgcggtg gtgcgcacgc cagcgccgtt
    28741 agccacgcca acaatcgctt cggcaagggc ttcagagagt tcgtaggtcg gcataaacgc
    28801 gccgctaccc actttcacgt ccatcaccag cgcgtccaga ccttccgcca gtttcttcgc
    28861 caggatcgag gcagtgatca gcgggatgga gtccacggtt gcggtaatat cacgggtcgc
    28921 gtagaaacgt ttatcagccg gagccagtga actggtctga ccgataatcg ccacgccgac
    28981 gtctttaata atttcgcgga aacggttgtc atccgggaaa atgtcgaagc cagggatgga
    29041 ttccagtttg tcgagcgtac cgccagtatg accgaggccg cgaccggaga tcatcggaat
    29101 atagccgccg caggctgcga ccatcggccc caacatcagc gaagtgacat cgccgacgcc
    29161 gccggtcgag tgtttatcaa caatcgggcc atccagatgc aggcttttcc agtcgagaac
    29221 ggttcctgaa tctcgcatcg ccatggtcag cgagacacgc tcaggcattg tcatatcgtg
    29281 gaagaaaatg gtcatcgcga gggcggcaat ctgcccttcg gaaatagtgt tgtcgcgaat
    29341 accgttgata aagaaacgaa tttcttcatc gctcagcgca tgaccatcac gttttttacg
    29401 aataatttct tgtgcgagaa acaaggtaac ctcccgagga aaagagtatg aggcggcttt
    29461 tgccggatgc gtctacgcct tatccggcct acgggtagat acgtctcgta ggcctgataa
    29521 gcgcagcgca tcaggcgtaa agcatcttac ttagtagctg ctggcgctct taccgtcgcc
    29581 gtggcccagc gctttcagca ggcttgccag caggctggaa gcaccaaagc ggtagtgacg
    29641 cgcatcagcc cagtcagcac cgaacagttc atctgcgatg gcgagatatt tctgcgcatc
    29701 ttccgcagta cgcacgccgc ccgccggttt gaaaccaacg gttttttcta cgcccatatc
    29761 acggatcact tccatcatga tgcgagcgct ttccggcgtc gcgttcacag ccactttacc
    29821 ggtagaggtt ttgatgaagt ccgcacccgc tttgatggag atttcagacg ctttacggat
    29881 cagcgcttcg tctttcagtt cgccggtttc gatgatcact ttcagcagta cattcgctgc
    29941 cgcgcaagcc tctttacagg ctttcaccag gtcaaaacca acctgctcgt cacccgccat
    30001 cagcgcgcgg tacgggaata ccacgtcaac ttcatcagca ccgtaggcga ttgccgcacg
    30061 ggtttctgcc agcgcgattt cgatgtcgtc gttaccgtgt gggaagttgg ttaccgtagc
    30121 gatacggatt tccggggtgc cctgctcttt cagagttttg cgagcaatcg ggataaaacg
    30181 aggatagata cagatagcgg cggtattgcc gaccggagtt ttggcctgat gacacagagc
    30241 aattactttc tcgtcggtgt cgtcgtcatt cagggtggtc aggtccatca atttcagtgc
    30301 acgcaggctg cttgctttca gatcagtcat ttcattctcc tggcttgtcg ccaataaaat
    30361 tcaccttgcg agtttgttag tattctaaca tctactccgc aacacacttc gatacacatc
    30421 acaattaagg aaatctactt acaagtttgc atcactgtaa tgcgatctgg ttcaaataat
    30481 tcactttcaa atgaatgcgt cagtggtggc aaacgcatca ggatcaaagt gaacatcacg
    30541 aaacttctta caatggcgca tcaccttcgg cataaaagga aaagaagatg cccacttctc
    30601 atgaaaatgc actgcaacaa cgttgccagc aaattgtcac cagcccagtg cttagcccgg
    30661 agcagaagcg ccattttctg gcactggaag cagaaaacaa tctgccttac ccacagcttc
    30721 ctgccgaagc ccgccgcgcg ctggatgaag gtgtaatctg cgatatgttt gaaggtcatg
    30781 cgccgtacaa accgcgctat gtcttacccg attacgctcg ttttctggcg aacggttccg
    30841 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
    30901 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt
    30961 tgttgcaacc gtatgttaga attctaacac aaaacgagat cgatgttcga ataaaacgtt
    31021 tctggcgtta cctcgacaga accctgccag atgcctttat gcacgccaat atcggcccgt
    31081 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc gcaggtttca ccgaacctga
    31141 cctttatcta cgatcctgaa atcacccctg atgacctgct gctggaagtg gcgaagaaca
    31201 tctgtgaatg tagcaaaccg cacatcgcca acggtccggt gcatgataaa attttcacaa
    31261 aagggggcta cgggattgtg agctgttaca actcactgcc gctggcgggt ggtggcagca
    31321 cgctggtacg ccttaacctg aaagccattg ccgagcgcag cgaatcgctg gatgacttct
    31381 ttacgcgcac tctaccgcac tactgccagc agcagatcgc catcatcgat acgcggtgtg
    31441 aattcctcta tcaacaatca cacttctttg agaatagctt cctggtgaaa gaagggctga
    31501 ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg gctggcggaa gcggttaact
    31561 tgctgtgtga gaaagaaggg attgccgcgc gctacggtaa agaagccgcc gcaaatgaag
    31621 tgggttatcg catcagcgcg caactggcgg agtttgtcgc caataccccc gtgaaatatg
    31681 gctggcaaaa acgcgccatg ttacacgcac agtcggggat cagttccgat atcggcacca
    31741 cgccgggcgc gcgtttgccg tatggcgatg agccagatcc gatcacccat ctgcaaactg
    31801 tcgccccgca tcatgcttat tattattccg gcatcagcga cattctgacg ctcgacgaaa
    31861 ccatcaaacg taacccgcag gcactggtac agctttgcct cggtgccttt aaagccggaa
    31921 tgcgtgaatt taccgccaat gtcagcggta acgatctggt tcgcgttacc ggttatatgg
    31981 tgcgtttgtc ggatttagaa aaatatcgcg ccgaaggttc acgcaccaac accacctggc
    32041 tgggcgaaga agccgcacgc aacactcgta ttctggaacg ccagccgcgc gtgataagcc
    32101 atgaacagca gatgcgcttt agtcagtaag attatcccct tctcctgcgt tgacgggcca
    32161 ggcagtcgtc tggctctgtt tttgcagggc tgcaatctgc gctgcaaaaa ctgtcacaat
    32221 ccgtggacga tgggacgttg caatgactgt ggggagtgcg tgccacagtg tccgcatcag
    32281 gcgttgcaga ttgttgacgg caaagtggtg tggaacgctg tggtctgcga gcaatgtgat
    32341 acctgcctga agatgtgtcc gcaacatgcc acaccaatgg cgcaatccat gagcgtggac
    32401 gaagtgctta gccatgtgcg caaagcggtg ctgtttattg aagggataac ggtaagcggc
    32461 ggcgaagtca cgacccaact gccgtttgta gtggcgctgt ttactgctat caaaaacgat
    32521 ccgcaactgc gccatctcac ctgtctggtg gacagtaacg gtatgttgag cgaaaccggc
    32581 tgggaaaaat tactcccggt gtgtgacggc gcaatgctcg atctcaaagc atggggtagc
    32641 gaatgtcatc aacaattaac cggacgcgat aatcaacaga ttaagcgcag catctatttg
    32701 ctggcagagc gcggcaagct ggcggaactg cgtttgctga tgatcccagg ccaggtggat
    32761 tatttgcaac acatcgaaga actggcggcg tttatcaagg gacttggaga tgttccggtt
    32821 cgcctgaacg cgtttcatgc ccacggcgtg tatggcgagg cgcaaagctg ggcgagcgcc
    32881 acgccggaag acgttgagcc gttggctgat gcattaaagg tgcgcggggt gagccggttg
    32941 atatttccgg cgctctattt gtgacatgcc ggatacggcc tacggttcgg cacagacttg
    33001 taggcctgat aagacgcgtc aagcgtcgca tcaggcattg tgcgccaact gccggatgcg
    33061 gcgtgaacgc cttatccggc ctacggttcg gcacaaacct gtaggcctga taagacgcgt
    33121 taagcgtcgc atcaggcatt atgcgccgac tgccggatgc tgcctacgaa gggcaaacta
    33181 cccacgaacg ttaaacaccg catacgtgtt attaagcaac acttcggcaa tctcatccgc
    33241 gggttccggg cgtaactcgc aaagcacggc aaacacacgg gcagcctgtt ccgggcggtt
    33301 aggctgcccc tgaaaaccgt tgagcggcat atccggcgca tcggtttcca gcaacaacga
    33361 tgccagcggt aattttgcga tgacatcgcg ggttttactg gcgcgtggat aggtgatagt
    33421 accgcctacg ccgattttgt agcccagctg tacaaaccgt tcagcctgtt gctggctgcc
    33481 agaaaatccg tggacaacac cagcacgcgg caaatcgtgg cgtttaagat gcatcgccag
    33541 tttgtcgtgc gtgcgccgtg aatgcaggat caccggcaga tcgtagcgtt tcgccagttt
    33601 cagttgttcg tcgagtaacc actgctgcct ctcaaattgc ggatcgtcgc cgaagagatc
    33661 cagaccgatc tcccccaccg ccaccacctt cgccggacgc ctttccagcg cctgctgtag
    33721 ctgctcaaga gacacatcgc tatgtttttc caacatacca ggatgcaagc ccaatgcggc
    33781 atacagcggt tgataatttt ccgctaacgc ctgcacgcgg gcaaaatttt ccgcctctgt
    33841 cgccggaaca atgatcttgc ctacgcccgc ttgtgccgcg cgttgcaggc tggcctcttc
    33901 atcgccaata aaaggcggga aatcaaaatg gcagtgagtg tcgataaaac ggcaaatcaa
    33961 gccagatcct cattgttaaa tgtcgggtcg ttcgcctgcg gagcgtcgct cacttcggca
    34021 accagcgtat cgttggcgac tggcgcaggc ggaatgacaa tcgattccgg cgtcaccact
    34081 ggcaccagat ggcgggtaag cggcgctttt tcagttaata gcttgccaac cgtggcgagg
    34141 aaataacgac cgcataaacg cccaagttta tagtcttcac gcagcgccgg aatccgactg
    34201 ccaagcgcga tactatgtaa tggcttcggc ggataaattt cggatatccg cagcttgccc
    34261 ggtggtttct caataaattg ctgaatgtca cgatagctgg tttcatgatg ctgcaccaga
    34321 ttgaccagcg gctgcaggct actgtcaccc agccagcgtt ccatgcgttt gaaccactgc
    34381 ggcgtgtagt acatttgtga cggcacagtg cggatgacga ccaacgtttt agcgccctgc
    34441 cttgccgcct ctttaaccgg aatcgcatca ctgatcccgc catccaggta gttaatgcct
    34501 tccagcgaca ctccgctacg ataaaagcca ggtatcgcac tggaggcgcg aatcacatcc
    34561 agccagtttt gtttggttgg taaaaagtaa ttcggcgcgt agtcatcctg acgacaggcg
    34621 cacatataaa acgatttgcc gctgtcaaac aaccgcgcgg cggtatccat ttgcaacggc
    34681 atctggcttg cggtggcctc caccagccag tcgagatcga taagatttcc tccacggaca
    34741 aagcgcaatg gatcaaaaaa ttcgcgtttt gtggtatagc gcatgatgac tttgcgcgcg
    34801 taaccgggct gattgcagat aaacgccgag aggttctgcg ccccggcaga tgtgccgaga
    34861 taaagatcga aaggattaaa ctgcgcgcgc ataaactcat ccagcacgcc agccgtgaaa
    34921 attccacgct gtccgccgcc ttcgcacacc agagctattc gtccaggctg gaacggcctt
    34981 agcgacaacg gcgcaatatt accaagcgtt acaggtattc tctgccccac ttctgccttc
    35041 ctgtttttgt tgtgaaagaa agacaaagta acgcaattta ttattcgcta aaaccgcaaa
    35101 agccagtccg ctggactggc tttggataaa attttcttaa tggtctgaaa tctagggtcg
    35161 ttttcggccc atgaacaaac tcactaggaa cagaataatc ccgacgacaa agacaatttt
    35221 agctgcgcct gcagcggtac cggccagacc accaaaccca agtgcggcgg cgattaacgc
    35281 gataaccaga aatatgatgc cccaacgaaa cataagcgtc tcctttacca tagttaatgt
    35341 caccgcttga tatgagcgtg aaaatcactc aatgcgatat ttttagtgtg gtgcacatcg
    35401 cgcctcccga caaagttcgg gaggacgaat tacgacgaat tacttagttt tcagatcatt
    35461 tttaacgctt ttcacaccat ctaccgcttt ggcgatactt tcagcacggt cactttgtgc
    35521 ctgagaatct acggtaccgg agagctgaac cacgccatcg gtggtttcaa ctttcacttt
    35581 acgtgaaggg acgatatcgt ccgccagcag tttggctttg atttcactgg tggtggcggt
    35641 gtcacccgcg tagtccttca ccgagccttc tttagcgtcg cgaacgtgca gtttgtcgct
    35701 gacagaggtc acgccttcaa cgcctttcgc cactttcact gcctcttcgg cctgggcctg
    35761 actttcaacg aaaccgctca gggtcacgac tttttgatcg gttttgacag agatatcggt
    35821 gctcttgatg ttgtcatgat ccaccagcgc cgccttcact ttcgcggtga tggcgctgtc
    35881 atccatgaaa ttaccgactt tattcataga gctatcgact ttttgccctg cgctttcatt
    35941 ggtagtctgc gcgttgtttt ccgcgtaggc agagccggtc gcgacggcag aggtcaacat
    36001 tacagccagc agagttttcg aaatcttcag tcttgtcata gtcatcgatt tattcctgta
    36061 tgtttgctcg taatttgagc ctagcaacac gaggttgcat tgctgaatag ggagagactt
    36121 caccctctac agaagtcaat ggtcgccatc acaaaagcga tgggtgatga ataacgacca
    36181 ttacagtctc tgaatcagtt attaatatcg gtagaatgac aatcgacggc tttagatact
    36241 gatatctacg cattgaacgg tatttaacgc catcagaaat gtcatcactt tgttaaatat
    36301 agatcacaat tttgaaaccg ctcgggatat cagcgaaaac ataagcaaaa gtgaatgttt
    36361 taagaacatt ccgtaagcgg ctaataagga agggaaattg acagggcgca gcggctaccg
    36421 cgccctggag gcaagaatta atgctcgcgg gtctggtgga actgaacgtc cggataacgt
    36481 tcctgtgcca ggcgcaggtt gaccatgctg gtagcgatgt aagcgaggtt atcgccgcca
    36541 tcaagcgcca gttggctttc gttcttacgc ttgaactctt cgaatttctt cgcgtctgca
    36601 cattctaccc agcgggcagt ggcaacgttg actgactcat acactgcttc aacgttgtat
    36661 tcgctcttca ggcgcgctac caccacatca aactgcagca caccaaccgc accaacgatc
    36721 agatcgttgt tggagattgg acggaacacc tgcaccgcgc cctcttcgga aagctgtacc
    36781 agccctttga gcagctgttt ttgcttcagc ggatctttca ggcggatacg acggaacagt
    36841 tctggtgcga agttcggaat accggtgaac ttcatcatct caccctgggt aaaggtgtcg
    36901 ccgatctgaa tggtgccgtg gttgtgcagg ccgaggatat cgcccggata cgcttcttca
    36961 acgtgcgaac ggtcacccgc cataaaggtc agcgcgtcgg agatcaccac atctttcgca
    37021 gtgcgcacct ggcgcagttt catgcctttt tcatatttac cggacaccac acgcataaac
    37081 gccacgcggt cgcggtgttt cgggtccatg ttggcctgaa ttttaaatac gaagccggta
    37141 aatttatctt cgctcgcttc tacggtacgg gtatcggtct gacgcggcat cggcgcaggc
    37201 gcccactcca ccaggccatt cagcatatga tcaacgccga agttacccag cgcagtaccg
    37261 aagaataccg gagtgatttc gcccgcaagg aacagctctt tgtcgaactc gttagacgcg
    37321 cctttaacca gttccagttc gtcacgcagc tgctgtgcca gatcttcacc aaccgcagca
    37381 tcgagatccg ggttattcag ccctttaaca atgcggactt cctgaatggt gtgcccttta
    37441 ccgctctgat agagataggt ttcatcttta taaaggtggt aaacgccttt aaacagcttg
    37501 ccgcagccaa ttggccaggt gatcggtgcg cagccaattt tcagctcgtt ctcaacttca
    37561 tcgagcagtt ccatcgggtc gcggatatca cggtcaagtt tgttcataaa ggtgaggatc
    37621 ggcgtgtcgc gcagacgggt aacttccatc agcttacggg tacgatcttc aacacctttt
    37681 gcggcgtcga taaccatcag gcagcagtcc accgccgtca gggtacgata ggtatcttcc
    37741 gagaagtctt cgtgccccgg ggtgtcgagc aggttaacca ggcaatcgtg atacggaaac
    37801 tgcatcacag acgtagtaat ggagatccca cgctgctttt ccatctccat ccagtccgac
    37861 ttagcgtgct ggttggaacc acggcctttt actgtaccgg cggtctgaat ggcctgtccg
    37921 aacagcagca ccttctcggt gatggtagtt ttaccggcgt ccgggtgaga aataatggca
    37981 aaagtgcggc gcttcgccac ctcttgcaaa taaggagaca acgtcataat tctttcttct
    38041 tgagtaagcg gcatcgcgcc gcgcttgttg aaaacgaaaa attgcggcta ttttacccat
    38101 caacgggggg gaggcaatca gtgtttacac aggagctgct ccagttcgtg caacgaagaa
    38161 acggtccagg tgggcgcgat gccttctggt tgctcgcgat tgtgcgcatt cagccagcag
    38221 gtcgcaagcc cggcgttgat gccaccgaga atatcggact cggcagtgtc gccaaccatc
    38281 agcacgcgtg aacggtcagg attgcccgcc tgttccagcg cataatcgaa aattttctta
    38341 ttcggtttgg caacgccaac ttcttcggaa atcaccagca aatcgaagta atcacgcagg
    38401 cccgtccgtt ccagacgcac ttgctgcaag gcgctaaaac cgttggtgat gatgccgatt
    38461 ttagcgttgc cacgaatggc gttaagcaga gaaaccgcgc ccggcaacgg cgtacagatt
    38521 tccgccatcg cattaataaa ggcatcgtta agcaagcctg cttcaacttt cagccgttcg
    38581 gcccagctct cgaaacgccc gtgctgaagc tgtaatgaag tgatcgcgcc gttttgataa
    38641 tccacccaca gtggcttgtt aacggcctga tagtcctgaa aatcttcagc ggtaaaggtg
    38701 acgctgtaat caagaaacat ccgctgcagg ccggtgaatg agtcaaaggt aaacagcgtt
    38761 tcatcggcat caaagaaaat ccagtcccac ttcattattc caccttgtct tacatactga
    38821 ttggcaacgc catgatgatg gcgtcttcgc gaccgtccgt ggtggggtag taattgcggc
    38881 gaatcgtcgc ctcgttaaag cctaaacttt cgtacagggc aatggcggca gcatttgagg
    38941 cacggacttc cagccatagt gtcgccacgc cgcgtttttc cagttcgtcg atcagatgtt
    39001 ccagcagcgc cctacccagc ccctgacgct gatagtcggg atcgaccgca atattgaaca
    39061 atgtagcttc atccagcacc acttgcgtaa tcgcaaacgc cgccattttg ccgttttgcg
    39121 ttaactgaaa gttgagataa cgctcgccct ggttgctggc aaaggttttt tcactccacg
    39181 gaaaggcgtg ggcgcgttgt tcaatgtggt aagccgctgg taaatcagtc gtttcgaggg
    39241 aagaaatcgt gttcatatgt gcaaatttgt tgccataacg cggcgcgtgc cgttgggttt
    39301 gcccgtaatt cggtgagcgc cggtgatgcc acctgagcgc cttccaatga tagcggttcg
    39361 tcagtaccca accgccagct gttgcagcga ctgccttgcg gcagcatcgc gattttttct
    39421 ggcgtcagtt gcagcacttg atcggggctg acggttaatg cgcgcagaac atcgctcact
    39481 aaaggatcag tcagggcggg aagatcgttt gccaccatca ccagacggac gtgtgccggg
    39541 ctcgcaatgg caatctcccc ctgcaacgcg ccagggcgac gcagcgacca ctgggtaatg
    39601 cccagttgct gtaactgcca gtctcgtcgg gatgtcatag cgaatcgctc ctgttgtcag
    39661 gggcgcaaat atagcaaatt cgtcgatacc gcgccaacat atggctataa tcgccgccag
    39721 tatcaattga ggagcattcc atgtctgcat ttaccccggc aagtgaagtc ttgctgcgtc
    39781 acagtgatga tttcgaacaa agccgtattc tgtttgccgg agacttacag gatgacctgc
    39841 ccgcgcgttt agataccgcg gccagccgtg ctcataccca gcaattccac cattggcagg
    39901 tgttaagctg ccagatgggg gataacgccc gttttagtct ggtcgccacg gcgaatgatg
    39961 tcgcagattg cgatacgctg atttactact ggccgaagaa caaaccggaa gcccagttcc
    40021 agttgatgaa tttactttct ctgctgccag tggggacaga tatttttgtc gttggcgaga
    40081 accgcagcgg cgtgcgcagc gctgagcaga tgctggcaga ttatgcgccg ttgaataaag
    40141 tcgacagcgc tcgtcgctgt ggcctctatt ttggtcgtct ggaaaaacag ccagtatttg
    40201 atgcggataa attctggggc gaatacagcg tcgatggcct gacggtcaaa acgctgcctg
    40261 gcgtgtttag ccgcgacggt ctggatgtcg gtagccagtt gctgctctcg acgttaactc
    40321 cgcacacgaa aggtaaagtg ctggatgtcg gctgtggcgc gggtgtactt tcagttgcct
    40381 ttgcgcgcca ttcgccgaaa attcgtctca ccttgtgcga tgtctctgcc ccggcggtag
    40441 aagccagccg cgcaacactt gcagccaacg gtgttgaagg tgaagtcttt gccagcaacg
    40501 tcttttccga ggtgaaaggt tgttttgata tgatcatctc caacccgccg ttccacgatg
    40561 ggatgcaaac cagcctggat gcggcgcaaa cgctgattcg tggcgcggtg cgtcatctta
    40621 atagcggcgg cgagctgcga attgtagcga acgccttcct gccttacccg gacgtgctgg
    40681 atgagacatt tggcttccat gaagttatca cgcaaaccgg gcgcttcaag gtatatcgcg
    40741 ccattatgac ccgccaggcg aagaaaggtt gagtatctga tatctgtcgc cgcataccgt
    40801 gtcggatgcg gcgtgaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
    40861 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    40921 cacgctagtt tcctgatgga catttttcca gcaattacac atcaggcgat aattaactat
    40981 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    41041 gatgg
//
LOCUS       DADLTF010000016        41033 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.016, whole genome shotgun sequence.
ACCESSION   DADLTF010000016 DADLTF010000000
VERSION     DADLTF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.016"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..221)
                     /locus_tag="JN749_000855"
     CDS             complement(<1..221)
                     /locus_tag="JN749_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HAY7367356.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIG"
     gene            complement(261..1106)
                     /gene="glpF"
                     /locus_tag="JN749_000856"
                     /pseudo
     CDS             complement(261..1106)
                     /gene="glpF"
                     /locus_tag="JN749_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            1532..1777
                     /gene="zapB"
                     /locus_tag="JN749_000857"
     CDS             1532..1777
                     /gene="zapB"
                     /locus_tag="JN749_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HAY7367357.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="JN749_000858"
     CDS             complement(1862..2347)
                     /gene="rraA"
                     /locus_tag="JN749_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HAY7367358.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(2440..3366)
                     /gene="menA"
                     /locus_tag="JN749_000859"
     CDS             complement(2440..3366)
                     /gene="menA"
                     /locus_tag="JN749_000859"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAY7367359.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="JN749_000860"
     CDS             complement(3433..4764)
                     /gene="hslU"
                     /locus_tag="JN749_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HAY7367360.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="JN749_000861"
     CDS             complement(4774..5304)
                     /gene="hslV"
                     /locus_tag="JN749_000861"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HAY7367361.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="JN749_000862"
     CDS             complement(5397..6356)
                     /gene="ftsN"
                     /locus_tag="JN749_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HAY7367362.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="JN749_000863"
     CDS             complement(6448..7473)
                     /gene="cytR"
                     /locus_tag="JN749_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HAY7367363.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            complement(7629..9827)
                     /gene="priA"
                     /locus_tag="JN749_000864"
     CDS             complement(7629..9827)
                     /gene="priA"
                     /locus_tag="JN749_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAY7367364.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            10030..10242
                     /gene="rpmE"
                     /locus_tag="JN749_000865"
     CDS             10030..10242
                     /gene="rpmE"
                     /locus_tag="JN749_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HAY7367365.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            complement(10303..10911)
                     /locus_tag="JN749_000866"
     CDS             complement(10303..10911)
                     /locus_tag="JN749_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HAY7367366.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="JN749_000867"
     CDS             complement(11095..11412)
                     /gene="metJ"
                     /locus_tag="JN749_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HAY7367367.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            11689..12849
                     /gene="metB"
                     /locus_tag="JN749_000868"
     CDS             11689..12849
                     /gene="metB"
                     /locus_tag="JN749_000868"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAY7367368.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            12852..15284
                     /locus_tag="JN749_000869"
     CDS             12852..15284
                     /locus_tag="JN749_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HAY7367369.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            15633..16523
                     /gene="metF"
                     /locus_tag="JN749_000870"
     CDS             15633..16523
                     /gene="metF"
                     /locus_tag="JN749_000870"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HAY7367370.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            16852..19032
                     /gene="katG"
                     /locus_tag="JN749_000871"
     CDS             16852..19032
                     /gene="katG"
                     /locus_tag="JN749_000871"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HAY7367371.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALLVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            19093..20031
                     /gene="yijE"
                     /locus_tag="JN749_000872"
     CDS             19093..20031
                     /gene="yijE"
                     /locus_tag="JN749_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HAY7367372.1"
                     /translation="MSTGKLHTGSLMSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVT
                     SYIGAFDFTALRCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWAL
                     VSGGAGKVAILSYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFS
                     SMKSAMLAILSGVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQ
                     REIDWQPTVFWALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWL
                     LGENPGAVEGSGIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(20058..20675)
                     /locus_tag="JN749_000873"
     CDS             complement(20058..20675)
                     /locus_tag="JN749_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HAY7367373.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="JN749_000874"
                     /pseudo
     CDS             complement(20950..22042)
                     /gene="gldA"
                     /locus_tag="JN749_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="JN749_000875"
     CDS             complement(22053..22715)
                     /gene="fsa"
                     /locus_tag="JN749_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7367374.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAEGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQTLLEMHAPES
                     MVLAASFKTPCQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="JN749_000876"
     CDS             complement(22727..25228)
                     /gene="ptsP"
                     /locus_tag="JN749_000876"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7367375.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRVRTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPREAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            25537..26616
                     /locus_tag="JN749_000877"
     CDS             25537..26616
                     /locus_tag="JN749_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HAY7367376.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            26631..26951
                     /locus_tag="JN749_000878"
     CDS             26631..26951
                     /locus_tag="JN749_000878"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7367377.1"
                     /translation="MTKIIAVTACPSGVAHTYMVAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            27002..29299
                     /locus_tag="JN749_000879"
     CDS             27002..29299
                     /locus_tag="JN749_000879"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7367378.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            29265..30143
                     /locus_tag="JN749_000880"
     CDS             29265..30143
                     /locus_tag="JN749_000880"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HAY7367379.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            30145..30486
                     /locus_tag="JN749_000881"
     CDS             30145..30486
                     /locus_tag="JN749_000881"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HAY7367380.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(30473..31324)
                     /locus_tag="JN749_000882"
     CDS             complement(30473..31324)
                     /locus_tag="JN749_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7367381.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(31473..33206)
                     /gene="cptA"
                     /locus_tag="JN749_000883"
     CDS             complement(31473..33206)
                     /gene="cptA"
                     /locus_tag="JN749_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HAY7367382.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="JN749_000884"
     CDS             complement(33389..36040)
                     /gene="ppc"
                     /locus_tag="JN749_000884"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HAY7367383.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLDYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(36392..37543)
                     /gene="argE"
                     /locus_tag="JN749_000885"
     CDS             complement(36392..37543)
                     /gene="argE"
                     /locus_tag="JN749_000885"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HAY7367384.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            37697..38701
                     /gene="argC"
                     /locus_tag="JN749_000886"
     CDS             37697..38701
                     /gene="argC"
                     /locus_tag="JN749_000886"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HAY7367385.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            38709..39485
                     /gene="argB"
                     /locus_tag="JN749_000887"
     CDS             38709..39485
                     /gene="argB"
                     /locus_tag="JN749_000887"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HAY7367386.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            39546..40919
                     /gene="argH"
                     /locus_tag="JN749_000888"
     CDS             39546..40919
                     /gene="argH"
                     /locus_tag="JN749_000888"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HAY7367387.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         9342 a        10530 c        11259 g         9902 t
ORIGIN      
        1 ataccgatag ctgcaatttg atcggaactg atatcggctt tcgccagcac ttctaccagc
       61 gtggagcttt gggtggccca gatttccatt gggtcgtgtt ctacccaacc tggttttggg
      121 tagatttgct caaattcgcg ctgcgacacg ctaatgatat tggcatcgtg atccattacg
      181 accgcgcggg agctggtggt gccctggtcg agcgcaacga tatatttttt tcagtcatgt
      241 ttaattgtcc cgtagtcata ttacagcgaa gctttttgtt ctgaaggagt tgtggtttcc
      301 ttttcttcca caacacagat atcgcaaggc aaatggcgac caatcagttt gcggtaggca
      361 aatgcaccta caatcgcgcc aacgataggg ccgaaaagcg gcaccaggaa gtaaggaatg
      421 tctctgccgc cggtaaaggc gacattgccc cagcccgcca gctaggcaaa gactttcgga
      481 ccgaagtcac gcgctgggtt catggcaaaa cctgtcaatg ggcccataga tgcgccaatg
      541 accgcaatca gtagaccaat cagcaaggga gccaaagggc cgcgtggtac accgttgcca
      601 tcgtccgtta acgccaggat aagccccatc agaatagcgg taatcaccat ctcaactgcg
      661 aaagcctgca caaaattgat atgaggatta gggtaagtag agaaagtgcc agccagatca
      721 acactttcaa cgctgccgcg aacaatgtga tgagtctgct cgaagtcgaa aaataaattg
      781 tagtaaagcc cgtaaactaa agccgcagcg cagaaagcgc cggcaacttg tgaaacgata
      841 aaaggaataa ctttgcgctt gtcgaaacag gcaaacagcc acaatgcaat ggtaacagcg
      901 ggattaagat gcgcgccgga aacccctgcg gtcaggtaga tggccattgc cacccccagt
      961 ccccaaatga cactgatttc ccactgacca aaagacgcac cagcgacttt tagtgctgca
     1021 acgcaaccca caccgaaaaa aatcaacaac ccggtaccga ggaattcagc aatgcactgg
     1081 cctttcaagg ttgatgtttg actcataatc ggatcctgaa gagttaatgt ttgttgtatg
     1141 tgtgaaagtc acggacctcc acgatgcttg taggcatgct gttaacttat cgttaacgag
     1201 caaaaacgag aaatatcgaa cttaaaatgt gtgtgcttcg tcataaaatg agcgttatcg
     1261 cgccatttat tccatctgat tgtacatgat gcatgtttgt tagatcattc gcgcaacaaa
     1321 tttattaaca ttttcgattg atgtggatta tgtcactagg agaaggatga aaagtgtggc
     1381 aaaccgtaat ctgcaaggcg tgccgctgga cagcgatggc gcggcttcat acaatcggag
     1441 ctaactaaag tgcgctcgta tttattaagg cgtcaccggt aatcgggacg aggatttttt
     1501 atccatcaac gccttgcaat tcaggagagg tatgacaatg tcattagaag tgtttgagaa
     1561 actggaagca aaagtacagc aggcgattga taccatcact ctgttgcaga tggaaatcga
     1621 agagctgaaa gaaaaaaaca actcactgtc gcaggaagtt caaaatgccc agcatcagcg
     1681 cgaagagctg gagcgtgaga acaaccatct gaaagaacag cagaacggct ggcaggaacg
     1741 tctgcaggcc ctgctgggtc gcatggaaga ggtctgatta cgcttcatct ttcgcgtaat
     1801 ttctttactg tgtaggccaa caggtaacgc agaaaaaagg caccttgcgg tgcctttctt
     1861 atcattcaat atccagcgga tcttctgaaa gaataatccc ggtattgtcg gcataaagat
     1921 ggtcgccgga gaagaaggtg acaccgccaa aattgacgcg gacatcgctt tcgccaatgc
     1981 cttcgccagc ggcaccaacc ggaattgccg ccatcgcctg gatgccgata tccaactctt
     2041 ccaggtcatc tacctgacgc accgcgccgt aaatgaccag accttcccat tcattttgta
     2101 ctgccagacg cgccagttca gcatcgacca gtgcgcgacg aacagaacca ccgccatcga
     2161 cgacaagaac acgaccacgg ccattctgtt cgagcagatc gtacagcaac ccgttgtcct
     2221 cgaaacattt taccgtgatt atttgtccgc caaacgacgc ccgtccgcca aagttggaga
     2281 acagcggttc cacgacgtta acatcttctt gatagatgtc acaaagctcg gaagtatcgt
     2341 atttcatagg cttaacattc agttgctgcg agaattttca gtatatcgcg ctatgtgggc
     2401 tgttggcaaa gtcatcaatt gttaattgat atttgtcagt tatgctgccc actggcttag
     2461 gaatatccct aaaacaaaca gcaggttagt cagtaacgct cccttgacag tacgttccag
     2521 cattggtcgc atcgccaccg ggtccatttc ccgcatcaca taacgggctt gcttcaccag
     2581 taatggtgcc gccagcagga acagccagcc ccacaggcta tgcagcgaaa agagattaaa
     2641 cagcgccaga cacaccagcg agcccatcag caggcaggca tgataacgac gcgcgttcac
     2701 ttcacctaag cgcaccacca gcgtgttttt gccattttcg cggtcgctat tgatatcacg
     2761 caggttatta atattcagta ctgccgttgc cagcaggccg catgcggtcg ccggaaggat
     2821 cagtgccgga atcaatgtat gagcctgtaa ataccagctc cccatgacac tcaaccagcc
     2881 aaagaaaacc agtacggaaa tatcacccag accgatataa ccataaggac gattgcccac
     2941 ggtgtaggtg atagcggcaa tgatcgacaa cccgccaaga atcaggaaac cgacaaaatc
     3001 ggccagcgta tggcatgcca ctgcaaccag tgccagcccg gagagacaga tgagcacgac
     3061 ggtaataatg agcgcccgtt tcatcttttg ctgggtaatg accccttttt gcatgccgcg
     3121 tagcggccca atgcggtcag gtttatcgct gccttttacc gcatcgccgt aatcattggc
     3181 gaggttagaa aggatctgta atagcccggc ggtaattagt gccagcaggg cgaccagcgg
     3241 atcgaagtga ccttgccacc atgccagcgc cgtcccgacg ataattgcag caaaggcgag
     3301 ggggagggtt ttaggtcgta aactttccag ccacgcctga gttcggctaa tttgttgttc
     3361 agtcataata cgcgccaata aaaatggggc ctttcagccc catcaaacaa tgatgaaaat
     3421 gattgaacgc gattatagga taaaacggct cagatcttca tctgccacca acgcatccag
     3481 atgtttgctc acataatctg cgtcaatagt gatattttga ccgcttaaat cgctggcgtc
     3541 gtaggaaatc tcttccatta aacgctccag aacagtgtgt aaacgacgag caccgatgtt
     3601 ttcggtagat tcgttcacct gccatgccgc ttccgcgatg cgtttaatac cggagtcggt
     3661 aaactcgata tttacgcctt cagtcgccat cagtgctttg tactgcacgg tgatagaggc
     3721 attcggctcg gtcagaatac gctcgaagtc gctggtggtc agtgcctgca gttcaacgcg
     3781 gattggcagc cgaccttgca gttccgggat caggtcagac ggtttcgcaa tctggaacgc
     3841 gccagaagcg ataaacagaa tgtggtcagt tttgaccatc ccgtgtttgg tggaaacggt
     3901 gcaaccttct accagcggca gcaggtcacg ctgaacgcct tcacgagaaa catccggacc
     3961 ggaagactcg ccgcgcttac agattttgtc gatttcgtcg ataaacacga tcccgtgctg
     4021 ctcaacagcg tcgatagcgt cttgcttcag ctcttccggg ttcaccagtt tcgccgcttc
     4081 ttcttcaatc agcagcttca tggcgtcttt gattttcagc ttacgcgctt tttgcttctg
     4141 gccgcccagg ttctggaaca tggactgcag ctggctggtc atctcttcca tgcccggagg
     4201 agccataatt tcaacgccca tcggtgctgc ggcaagatcg atctcgattt ctttgtcatc
     4261 aagctggcct tcacgcagtt ttttgcggaa tgcctgacga gcagcggaag gttcctgctg
     4321 ctgttcagtc tgtccccagt tgtttttagc aggtgggatc agcacgtcga gaatacgttc
     4381 ttctgccagt tcttcagcgc gataacggtt tttctcgata gcctggacgc gtaccatttt
     4441 cacggcggca tcggtcagat cgcgaataat agaatcaact tccttaccga cgtagcccac
     4501 ttcggtaaat ttggtcgctt caactttgat gaacggcgca ttcgccagct tagccagacg
     4561 acgggcgatt tcagttttac cgacaccggt cgggccgatc atcaggatat ttttcggggt
     4621 cacttcatgg cgcagctctt cgttgagctg catgcgacgc cagcggttac gcagagcaat
     4681 cgccacagaa cgcttggcgt tgtcctggcc gatgatgtgc ttatccagtt cgctgacgat
     4741 ttcgcgtggg gtcatttcag acatgggaga tccttacgct ttgtagctta attcttcgat
     4801 ggtgtggaaa tggttggtat agatgcaaat gtcgcctgca atatccaacg ccttttcagc
     4861 aatttcacgg gcgctaagtt cagtgttttc taacagcgcg cgcgccgcag cctgggcgta
     4921 agggccgccg gagccgatag caataagatc gttttctggc tgcaccacgt caccgttacc
     4981 ggtgatgata agcgatgcag tttcatccgc gactgccagc agtgcttcaa gtttgcgcag
     5041 catgcgatcg gtacgccagt cttttgccag ctcaacggcg gctttaacca gatggccctg
     5101 atgcatttcc agtttacgtt caaacagttc gaacagcgta aaagcatccg cagtaccgcc
     5161 cgcaaagccc gcgatgactt tgtcgttgta cagacggcgg acctttttca cgttgccttt
     5221 cattacggta ttgcccaacg tggcctgacc atcaccagcg atgaccacat ggccgttacg
     5281 gcgtacgctt actatagttg tcacgagctg accccttggt tacgaataca gagtacaaac
     5341 cccgtacaaa agtacggggc ataatgcaat tatagatggg ggggattttg agggtttcaa
     5401 cccccggcgg cgagccgaat gcagtttgta tgacccgcca tcttcaaccg attgagggtg
     5461 ctgtctgcgt tctctttgcc tttcaccggg ccaatgacca cacgattcca gccattgttg
     5521 gtggtgattt tcgagtcaaa tccttcgaac gccagctgag cacgtaccgt ctctgcctgt
     5581 tccgcgcctc tgaacgaacc gcactgcacc atccagcggc gttcgtcttt tttctccgcc
     5641 gtcggttttg gcgcgtcagc ggcacgagca accggcgcag cttgctgcgg cttcgactgc
     5701 gcggtcgtgt gcgcaggggt ttgcagcaga tcctggtacg gctgctgagt agaagctggt
     5761 ttagactggc gcggttgcgc ctgtaccggc gcggcttgcg acgtacgcgt ctgctgctgc
     5821 cagctttgtt cagtcgtacg cgactgctga gccaggcgtt gctgctccgc taattgctgc
     5881 gcctgacgct ggcgttgtag cgtttgctga cgctgttccg gcgtctgttc attccacgga
     5941 acctcaacca gctgcgttgg ctgctggcgc atatcagcct gcatttgttc aagaagctga
     6001 cgttgttctg gtgtcagttg ctccggcgtt ttcacttcac caccggcaga aggttctgtg
     6061 ggcgcacgca cacccggctg gcgactttcc agctctttaa tgtagcgcca gcgttcttct
     6121 ggttttggtg gtagtccgtt tccggtcact ttctggcttt gcagcgtctc ggactcttct
     6181 ttcttgtgat gcgtaatgaa gtacagacca ccgataaagg tcacaagaac ggcggcagca
     6241 atagcgacca tagcgggaga aaccgcaggc agatttcgtt gctttttccg tgaggtgctc
     6301 ttttttcgcc gcgaaggtgc cggttggctg cggcgtacat aatctcgttg tgccactatc
     6361 gtttcgctgt atttattcgt tcgtcagccc gccatgttac ttaagcggcg ggcctttgac
     6421 cagtcacggc agtcttaaag gtttacttta aggtaacgcg cgtgttgatc cccggatgat
     6481 aagttcgcag tccattaaac gagagccact gccaacgtgt tgcccctgca tttgatcaag
     6541 caataacagc atagcttccc gaccgatttc gtaacgcggc tgcgcgatgg ttgtcagcgg
     6601 cggatcacaa aattgcgtca ggtcgatgtt atcaaaaccg attatggaaa ggtcttccgg
     6661 gactttcagc ccctggcgtt ttgcctgaga aagtgcgccg agcgccatca catcgctatg
     6721 gcagaagact gcagtaggcg gttgtggaag atcaagcagc tgttgcattg ctttgcttcc
     6781 ggcttcgaag gtgaagtcgc cacgggcgat gtattgtgga tcaaccataa ttccgcagcg
     6841 acgcagcgcc tgaacatagc cttgcaggcg gtagtgacac agtggcatct cttcgggacc
     6901 ggctatacag ccaatccgtt tatgcccttg ctcatataaa taatttactg catcaaatgc
     6961 ggcggtcaga ttgtcgatat gaacggtagg cagctccagt tccggtgcaa attcgttcgc
     7021 catcaccatc ggcggcagat tacgctgttc ctcaatgctg gcatcaaacg gcagccttga
     7081 acccagcagc aacatgccat caatttgctt ggtgatgatc aaatcgataa aggttttttc
     7141 ctgctgattt tgatgcgcac agtcgccaat cagcaccaga tatccgtgat ttgccgccgt
     7201 aacttcgata ccgcgaataa tttcgctaaa gaagggatcg cagatatccg ggacaatcac
     7261 cagaatggtg cgggattcat tacgcttgac gttgcgcccc ataggctgcg gtaaataacc
     7321 cacttcccgg gccgcttttt caacccgatt acgggtggcc tgggagactt tatcgggatt
     7381 cattaatgct cgggagacag tcgctgtaga gacttttgcc ttgagggcaa cgtctttcat
     7441 ggtcgcggca gtttcctgct tcttcgcttt cacactcact cctcgcctgg cacgtcaggc
     7501 gtactacatc catgtttact tcacatcggc aacattttta gcagatagcg cgtgaaaacg
     7561 gttacagaat tttcatgaaa agtgtgatga atattgaatt tttcgatccg cctcgcatcg
     7621 taagcggttt aaccctcaat cggatcaaca tccagtaccc atttcacctt gcgggaatcc
     7681 ggtattgtat tgatgagcgc cagcgtacca ctaatgatgt gttgcaagcg cacgcgggaa
     7741 gggtgctgca acaatatctg ccagcgccag cggccgccac gtttaggtgc cagtgccgga
     7801 accggaccga gaacccacag tttgtcgtct gccagcggac tggagaggat cagattacgc
     7861 agttgttgca ggaacagcgg cgcatgctga ttgttatgat cttccgcacg cacaatcaca
     7921 tggctggtcc atggcggcag ttgcatcatc cgtcgctcag ccagcgcctg ttcggcaaag
     7981 gcgtcgtagc ctttatagag caacgtttgc aacagaggat gttccggatg gtgcgtttgc
     8041 agcaccactt cgccttgttt acccgcacgc ccggcacgac cagcgacctg ggtgtaaagc
     8101 tgagcgaaac gctctgccga gcgaaaatcg gcagaaaaca gcgcgccgtc cacatccagt
     8161 aacgccacca gcgtcacatc cggaaagtgg tgacctttcg ccagcatttg tgtgccgatc
     8221 aaaatcctcg caccgccgcg atgtacttcc gccagttgct gttccagcgc ccctttgcgg
     8281 ctggtggtat cgcggtcgat acgagaaatg ggcacatcag ggaacagcgg cgcaagcgtc
     8341 tgttcaagct gttcggtgcc cagccccacg ggtaccaggt gcgtggaacc gcaggaaggg
     8401 cactggcgcg gcaccggacg ctgactgtca cagtggtgac agcgcaaatg ctgttgcgcc
     8461 tgatgcagcg tgtagtagtg atcgcaacgt gggcattcgg caatccagcc gcagtcgtgg
     8521 cacagtagcg caggcgcaaa gccacggcgg ttaagaaaga ggataacctg attgttggcc
     8581 tgtaaatgct ggcgcatacg ggtgattaac gccggagcca gacctgcctg caccttctga
     8641 ccttttaaat ccagcacgtg ttgaattgcc ggacgcgcat tccccgcccg gcgcgtcagg
     8701 cgcagcaggc ggtatttttt ctgctggacg ttgcacaacg tttccagcgc gggcgttgcg
     8761 gagccaagaa taatcgggat ttgctcgcta tgcgcacgat acaccgccag gtcgcgggca
     8821 tgatagcgcc agccttcctg ctgcttgtag gaactgtcgt gctcttcgtc aatgacaatc
     8881 acgccgagat ttttaaacgg cgtaaacagc gcggagcggg tgccgatcac aatcgccgcc
     8941 tcgccatttt tcgctttcag ccacgccgaa agacgctcgc tgtcgttcag accggaatgc
     9001 agaacttcca cgggcgcatt aaaccgttca cgaaaacggg cgatagtttg cggtgtcagg
     9061 ccgatttccg gcaccatcac cagcgcctgt ttgccctgag cgagcacgtt ttccagtaca
     9121 ctgagataaa cctccgtttt accggaaccg gtaacgcccg ccagcagcca ggcagaaaaa
     9181 gtatctgccg cgctatgaat tgcgccaacg gcggtggcct gttcggtatt caatcgcaac
     9241 cgctcaccag aaacggcata gttcgttcgc cagtcgctaa actctggtgt ttcacttgct
     9301 aaatcacaca gacctttttt gcgcagcgcc tgcaacgcgg catcattaaa ttcgagcgtg
     9361 gcaacctggt cgcgccagat tttgccttgc cgtaacgctg ccagcgcctg ttgttgcttg
     9421 ggtgagcgtt tcaggctgtt cagatccacc gcctgacctt gttcagtggc aaaccagtac
     9481 cacatcggcg cgttcgccgc aggccgcccc tggcgtagta aaatcggcaa ggcatgaaac
     9541 agcacatcgc caatcggatg atgatagtaa tctgccgccc atagcagcaa tcgccagacg
     9601 gagtgagtaa acaccggctc accatccagc acttcgacta ccgcttttag ctcagtgagc
     9661 ggcagttcgc tggcatcgct aactgatacc acaatcccga tgcgctcctg ctgtttgcca
     9721 aacggcacgc gcacgcgaca cccagcttta accgtcatgc cttctggcag cagatagtca
     9781 aaggtacgag gaagcggaac gggcaaggca acgtgggcaa cgggcatagc atcatcctga
     9841 cttgaaattc ggtgggttag tatacacatt gccgtagaag agtgcggatc agtttgcata
     9901 cgctggttaa tttctgtatg atttcgcgcc ttcgtacgaa atgatcgtat tgaagctata
     9961 cttttaacat cgcgtggtgt ctggcgttag ggctggaaga gcgacgcggc cttaaaccga
    10021 ggttttccca tgaaaaaaga tattcacccg aaatacgaag aaattactgc tagctgctct
    10081 tgcggtaacg taatgaaaat ccgctccacc gttggtcatg acctgaacct cgacgtgtgc
    10141 agcaagtgcc acccgttctt cactggcaaa cagcgtgatg ttgctaccgg tggccgtgtt
    10201 gaccgcttca acaagcgttt caacatcccg ggcagcaaat aagaccggat ttccgaaaaa
    10261 agcgccgcac ggcgcttttt ttgtgcctga attttggtcg tatcaccacg aaaacagcgg
    10321 ccactctttg gcaaccgtgg tgagttgtgg cgcgtcgaaa acggcttgtg gtgaaactac
    10381 cgtttcctca agcggaatat ttttaccgta gcgttctttg agcttcgcct ggaccagcgg
    10441 gttgctgaga tcaaactctt tcaacttctg ctgttcgccc acgcgcattc ccagcgcgtt
    10501 ctggtaaacc ttccacacca cttccgaaca gtactggcga tcgtcgctcc atgagaagct
    10561 gaaatcatac ggtttaccga gataacgttt tgccgtttgc gtcagttttt gctgttgttc
    10621 aacactcagt ccgccttcaa cgcggcgaac aacgtagttg cccttttcac catgggcaat
    10681 ccactgcttg agcggggtgt atttcaccgg gccgaccgct tcaaaaacgt agggcttttt
    10741 gttacgcatc accagcatac cggtgtggct gtaatcggaa tgggttgcca gttggatcgc
    10801 tttactttgc gatgagcgag agatttgaaa gatgatgtcg ccagtttgcg gctgccaggc
    10861 aaaggcaggt acagaaacca gcaggctgag gatcagcagc ctgtttttca tagttaaacg
    10921 tccatgtata aaaagcggtg ggtcgcagac aacgtgctcg ttgtttatgc cggatgcggc
    10981 gtgaacgcct tatccggcct acaagttcgt gcaaattcaa taaattgcaa tatgacgtag
    11041 gcctgataag cgtagtgcat caggcgattc cactccgcgc cgctcttttc cgctttagta
    11101 ttcccacgtc tccgggttaa tccccatctc acgcatgatc tcttttgccg cttccgggat
    11161 ttcgtcgctg cgctctttac gcagatcggc atcatccggc aaaggttgcc cggtaaaggc
    11221 atgcagaaac gcttcgcaca gcagctcgct gttggtagcg tgacgcaggt tgttcacctg
    11281 acgacgcgtg cgttcatcgg tgaggatttt taacacctta agaggaatgg aaaccgtaat
    11341 ctttttgact tgttcactct tcttgccgtg ttcagcgtat gggctgatat attcgccgct
    11401 ccattcagcc atgagatact taatcctctt cgtcaataaa ttgagaccag accccacaat
    11461 ttatgtgggt actgaccgta aacccgcata gtttaccgta caggcgttac cgtgacatcg
    11521 tgtaatgcac ctgtcggcgt gataatgcat ataattttaa cggctatttg ggatttgctc
    11581 aatctatacg caaagaagtt tagatgtcca gatgtattga cgtccattaa cacaatgttt
    11641 actctggtgc ctgacatttc accgacaaag cccaggaaac ttcatcacat gacgcgtaaa
    11701 caggccacca tcgcagtgcg tagcgggtta aatgacgacg aacagtatgg ttgcgttgtc
    11761 cctcccatcc atctttccag cacctataac tttaccggat ttaatgaacc gcgcgcgcat
    11821 gattactcgc gtcgcggcaa cccaacgcgc gatgtggttc agcgtgcgct ggcagaactg
    11881 gaaggcggtg ctggcgcagt acttactaat accggcatgt ccgcgattca cctggtaacg
    11941 accgtctttt tgaaacctgg cgatctgctg gttgcgccgc acgactgcta cggcggtagc
    12001 tatcgcctgt tcgacagtct ggcgaaacgc ggttgctatc gcgtgttgtt tgttgatcaa
    12061 ggcgatgaac aggcattacg ggcagcgctg gcagaaaaac ccaaactggt actggtagaa
    12121 agcccaagta atccattgtt acgcgtcgtg gatattgcga aaatctgcca tctggcaagg
    12181 gaagtcgggg cggtgagcgt ggtggataac accttcttaa gcccggcatt acaaaatccg
    12241 ctggcattgg gtgccgatct ggtgttgcat tcatgtacga aatatctgaa cggtcactct
    12301 gacgtggtgg ctggcgtggt gattgctaaa gacccggacg ttgtcactga acttgcctgg
    12361 tgggcaaaca atattggcgt gacgggcggc gcgtttgaca gctatctgct gctacgcggg
    12421 ttgcgaacgc tggtgccgcg tatggagctg gcgcagcgca acgcgcaggc gattgtgaaa
    12481 tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc cgtcgctgcc ggaaaatcag
    12541 gggcatgaaa ttgccgcgcg tcagcaaaaa ggctttggcg caatgttgag ttttgaactg
    12601 gatggcgatg agcagacgct gtgtcgtttc ctgggcgggc tgtcgttgtt tacgctggcg
    12661 gaatcattag ggggagtgga aagtttaatc tctcacgccg caaccatgac acatgcaggc
    12721 atggcaccag aagcgcgtgc tgccgccggg atctccgaga cgctgctgcg tatctccacc
    12781 ggtattgaag atggcgaaga tttaattgcc gacctggaaa atggcttccg ggctgcaaac
    12841 aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa aggtcgtcag ctgcataaat
    12901 ttggtggcag tagtctggct gatgtgaagt gttatttgcg tgtcgcgggc attatggcgg
    12961 agttctctca gcctgacgat atgatggtgg tttccgccgc cggtagcacc actaaccagt
    13021 tgattaactg gttgaaacta agccagaccg atcgtctctc tgcgcatcag gttcaacaaa
    13081 cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct acccgctgaa gaagccgata
    13141 gcctcattag cgcttttgtc agcgatcttg agcgcctggc ggcgttgctc gacagtggta
    13201 ttaacgatgc ggtgtatgcg gaagtggtgg gccacggtga agtgtggtcg gcgcgcctga
    13261 tgtctgcggt acttaatcaa caagggctgc cagcggcctg gcttgatgcc cgtgagtttt
    13321 tacgcgctga acgcgctgcg caaccgcagg ttgatgaagg gctttcttac ccgttgctgc
    13381 aacagctact ggtacaacat ccgagcaaac gtctggtggt gaccgggttt atcagccgta
    13441 acaacgccgg tgaaacggtg ttgctggggc gtaacggttc cgactattcc gcgacacaaa
    13501 tcggtgcgct ggcgggtgtt tctcgtgtaa ccatctggag cgacgtcgcc ggggtataca
    13561 gtgccgaccc gcgtaaagtg aaagatgcct gcctgctgcc gttgctgcgt ctggatgagg
    13621 ccagcgaact ggcgcgtctg gcggctcccg ttcttcacgc ccgtacttta cagccagttt
    13681 ctggcagcga aatcgacctg cagctgcgct gtagctacac gccggatcaa ggttccacgc
    13741 gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat tgtcaccagc cacgatgatg
    13801 tctgtttgat tgagtttcag gtgcccgcca gtcaggattt caaactggcg cataaagaga
    13861 tcgaccagat cctgaaacgt gcccaggtgc gtccactggc ggttggcgtg cataacgatc
    13921 gccagttgct gcaattttgc tacacctcag aagtggccga cagtgcgctg aaaatcctcg
    13981 acgaagcggg attacctgga gaactgcgcc tgcgtcaggg gctggcgctg gtggcgatgg
    14041 tcggggcggg tgtcacccgt aacccgctgc attgccaccg cttctggcag caactgaaag
    14101 gccagccggt cgagtttacg tggcagtccg atgacggcat cagtctggtg gcagtactgc
    14161 gcaccggccc gaccgaaagc ctgattcagg ggctgcatca gtccgtcttc cgcgcagaaa
    14221 aacgcatcgg cctggtattg ttcggcaaag gcaatatcgg ctctcgctgg ctggaactgt
    14281 tcgcccgcga gcagagcacg ctttcggcgc gtactggctt tgaatttgtg ctggcaggcg
    14341 tggtggacag ccgccgcagt ctgttgagtt atgacgggct ggacgccagc cgcgcgttag
    14401 ccttcttcaa cgatgaagcg gtcgagcagg atgaagagtc gcttttcctg tggatgcgcg
    14461 cccatccgta tgatgattta gtggtgttgg acgttaccgc cagccagcag cttgctgatc
    14521 agtatctcga tttcgccagc cacggtttcc acgtcatcag tgccaacaaa ctggcgggag
    14581 caagcgacag caataaatat cgccagatcc acgacgcctt cgaaaaaacc gggcgtcact
    14641 ggctatacaa cgccaccgtt ggtgcgggct tgccgatcaa ccataccgtg cgcgatctga
    14701 tcgacagcgg cgataccatt ttgtcgatca gtgggatctt ctccggcacg ctctcctggc
    14761 tgttcctgca attcgacggt agcgtgccgt ttaccgagct ggtggatcag gcgtggcagc
    14821 agggcttaac cgagcctgat ccgcgtgacg acctctccgg caaagacgta atgcgcaagc
    14881 tggtgattct ggcgcgtgaa gcgggttaca acattgaacc ggaccaggta cgtgtggaat
    14941 cgctggtgcc tgctcattgc gaaggtggca gcatcgacca tttctttgaa aatggcgatg
    15001 aacttaacga gcagatggtg caacggctgg aagcggcccg cgaaatgggg ctggtgctgc
    15061 gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt gggcgtggag gcggtgcgtg
    15121 aagaccatcc gctggcatca ctgctgccat gcgataacgt ctttgccatc gaaagccgct
    15181 ggtaccgcga taatccgctg gtgatccgtg ggcctggcgc tgggcgcgac gtcaccgccg
    15241 gggcgattca gtcggatatc aaccggctgg cacagttgtt gtaatttcag aaatttaata
    15301 atgcccggta ctcatgtttt cgggtctatg gtttctaatg aaatatattg aattatcata
    15361 gaattaggcc ggatgaagcg tttacaacga atccggcaag aagcaataag tacatggtta
    15421 gtttatattt gcagtccggt ttgctttgca tatcggattt tctttttctt accatcctga
    15481 agttttttca tcttccctga tttttcctca ccatcattgg tcatttttcg gttgacgccc
    15541 ttcggctttt ccttcatctt tacatctgga cgtctaaatg gatagatgtg cacaacacaa
    15601 catataacta caagcgattg atgaggtaag gtatgagctt ttttcacgcc agccagcggg
    15661 atgccctgaa tcagagcctg gcagaagtcc aggggcagat taacgtttcg ttcgagtttt
    15721 tcccgccgcg taccagtgaa atggagcaga ccctgtggaa ctccatcgat cgccttagca
    15781 gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa ctccggcgag cgcgaccgta
    15841 cgcacagcat tattaaaggc attaaagatc gcactggtct ggaagcggca ccgcatctta
    15901 cttgcattga tgcgacgccc gacgagctgc gcaccattgc acgcgactac tggaataacg
    15961 gtatttgtca tatcgtggca ctgcgtggcg atctgccgcc gggaagtggt aagccagaaa
    16021 tgtatgcttc tgacctggtg acgctgttaa aagaagtggc agatttcgat atatccgtgg
    16081 cggcgtatcc ggaagttcac ccggaagcaa aaagcgctca ggcggatttg cttaatctga
    16141 aacgcaaagt ggatgccgga gccaaccgcg cgattactca gttcttcttc gatgtcgaaa
    16201 gctacctgcg ttttcgtgac cgctgtgtat cggcgggcat tgatgtggaa atcatcccgg
    16261 gcattttgcc ggtatctaac tttaaacagg cgaagaaatt tgccgacatg accaacgtgc
    16321 gtattccggc gtggatggcg caaatgtttg acgggctgga tgacgatgcc gaaacccgca
    16381 aactggtggg cgcgaatatt gccatggata tggtgaagat tttaagccgt gaaggagtga
    16441 aagatttcca cttctatacg cttaaccgcg ctgaaatgag ttacgcgatt tgccatacgc
    16501 tgggtgttcg acctggttta taaatagtgt ggcttttgtg aaaatcacac agtgatcaca
    16561 aattttaaac agagcacaaa atgctgcctc gaaatgaggg cgggaaaata aggttgtcag
    16621 ccttgttttc tccctcatta cttgaaggat atgaagctaa aacccttttt tataaagcat
    16681 ttgtccgaat tcggacataa tcaaaaaagc ttaattacga tcaatttgat ctacatctct
    16741 ttaaccaaca atatgtaaga tctcaactat cgcatccgtg gattaattca attataactt
    16801 ctctctaacg ctgtgtatcg taacggtaac actgtagagg ggagcacatt gatgagcacg
    16861 tcagacgata tccataacac cacagccact ggcaaatgcc cgttccatca gggcggtcac
    16921 gaccagagcg cgggggcggg cacaaccact cgcgactggt ggccaaatca actccgtgtt
    16981 gacctgttaa accaacattc taatcgttct aacccgctgg gtgaggactt tgactaccgc
    17041 aaagaattca gcaaattaga ttactacggc ctgaaaaaag atctgaaagc cctgctgaca
    17101 gaatctcagc cgtggtggcc agccgactgg ggcagctacg ccggtctgtt tattcgtatg
    17161 gcttggcacg gcgcaggtac ttaccgttca atcgatggac gcggcggcgc gggtcgtggt
    17221 cagcaacgtt ttgcaccgct gaactcctgg ccggataacg taagcctcga taaagcgcgt
    17281 cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa tctcctgggc cgacctgttt
    17341 atcctcgcag gtaacgtggc gctggaaaac tccggcttcc gtaccttcgg ttttggtgcc
    17401 ggtcgtgaag acgtctggga accggatctg gatgttaact ggggtgatga aaaagcctgg
    17461 ctgactcacc gtcatccgga agcgctggcg aaagcaccgc tgggtgcaac cgagatgggc
    17521 ctgatttacg ttaacccgga aggcccggat cacagcggcg aaccgctttc tgcggcagca
    17581 gctatccgcg cgaccttcgg caacatgggc atgaacgacg aagaaaccgt ggcgctgatt
    17641 gcgggtggtc atacgctggg taaaacccac ggtgccggtc cgacatcaaa cgtaggtcct
    17701 gatccagaag ctgcaccgat tgaagaacaa ggtttaggct gggcgagcac ttacggcagc
    17761 ggcgttggcg cagatgccat tacctctggt ctggaagtag tctggaccca gacgccgacc
    17821 cagtggagca actatttctt cgagaatctg ttcaagtatg agtgggtaca gacccgcagt
    17881 ccggctggag caatccagtt cgaagcggta gacgcaccgg aaattatccc ggatccgttc
    17941 gatccgtcga agaaacgtaa accgacaatg ctggtgaccg acctgacgct gcgttttgat
    18001 cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc cgcaggcgtt caacgaagcc
    18061 tttgcccgtg cctggttcaa actgacgcac agggatatgg ggccgaaatc tcgctacatc
    18121 gggccggaag tgccgaaaga agatctgatc tggcaagatc cgctgccgca gccgatctac
    18181 aacccgaccg agcaggacat tatcgatctg aaattcgcga ttgcggattc tggtctgtct
    18241 gttagtgagc tggtatcggt ggcctgggca tctgcttcta ccttccgtgg tggcgacaaa
    18301 cgcggtggtg tcaacggtgc gcgtctggca ttaatgccgc agcgcgactg ggatgtgaac
    18361 gccgcagccg ttcgtgctct gcttgttctg gagaaaatcc agaaagagtc tggtaaagcc
    18421 tcgctggcgg atatcatcgt gctggctggt gtggttggtg ttgagaaagc cgcaagcgcc
    18481 gcaggtttga gcattcatgt accgtttgcg ccgggtcgcg ttgatgcgcg tcaggatcag
    18541 actgacattg agatgtttga gctgctggag ccaattgctg acggtttccg taactatcgc
    18601 gctcgtctgg acgtttccac caccgagtca ctgctgatcg acaaagcaca gcaactgacg
    18661 ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc gtgtactggg tgccaacttc
    18721 gatggcagca aaaacggcgt cttcactgac cgcgttggcg tattgagcaa tgacttcttc
    18781 gtgaacttgc tggatatgcg ttacgagtgg aaagcgactg acgaatcgaa agagctgttc
    18841 gaaggacgtg accgcgaaac cggcgaagtg aaatacacag ccagccgtgc ggatctggta
    18901 tttggttcta actctgtcct gcgtgcagta gcggaagttt acgccagcag cgatgcccac
    18961 gagaagtttg ttaaagactt cgtagcggca tgggtgaaag tgatgaacct cgaccgtttc
    19021 gacctgctgt aatctgacct tcttcagcga ctgtctttca ggcagtcgct gaagtttctt
    19081 taccggcgta tagtgtccac aggaaaacta cacactggat ctctcatgtc tgccgcagga
    19141 aagagcaacc cactggcaat cagtggcctg gttgtgctca cacttatctg gagttatagc
    19201 tggattttca tgaagcaagt caccagttac atcggtgcct tcgactttac cgccttacgc
    19261 tgcattttcg gcgctctcgt tttattcatc gtccttttat tacgtggtcg cggaatgcgc
    19321 ccgacgccgt ttaaatacac cttagccatt gccctgttac aaacctgcgg gatggttggt
    19381 ctggcgcagt gggcgttggt cagcggaggt gcggggaagg tggcgatcct gagctatacc
    19441 atgccgttct gggtggtgat tttcgccgcg ttgtttctcg gtgaacgcct gcgacgtggg
    19501 caatatttcg cgattctgat tgccgctttc ggcttatttt tggtgttgca gccgtggcaa
    19561 ctcgatttct cttcgatgaa aagtgccatg ctggcaatcc tctccggtgt tagttggggg
    19621 gcgagcgcga ttgttgctaa acgcctatac gcccgtcatc cgcgcgtgga tttattatcg
    19681 ttaacatcct ggcagatgct gtatgcggcg ctggtgatga gtgtggtcgc tttactggtg
    19741 ccgcaacgtg aaattgactg gcagcccacc gtgttctggg cgctggccta cagtgcgatt
    19801 ctggcgacgg cgctagcgtg gagcttatgg ctgtttgtgc tgaaaaactt gcctgccagt
    19861 attgccagct taagcacact ggccgttccc gtttgtggcg tactcttttc ctggtggctg
    19921 ctcggtgaga atccgggggc cgttgaaggt agcggtattg tgctgattgt gctggcactg
    19981 gcgctggtga gccgtaagaa aaaagaagct gtcagtgtaa aaagcatctg aattttttct
    20041 tcatgtgggg cgatctctta tttaacaaaa taacgataat gccccaccat ccgccagcta
    20101 aacagcacat cttcttcctg cgcgcctgcg ccaatgttat gtatcaccag cggcgtaccg
    20161 tcgcgggcga agccatctga cgccacccca atatgcgcca gcccgttatc taatcgccag
    20221 gagacaatat cgcccgcttg atagtcaccg gcatttttac tgatggggcg tgttttgttg
    20281 tggcggctaa accaggtttc cagattaggc acccgacggt gatcgatgtt gctgtccggg
    20341 cgctttaact tccatttttg cgggtactca gcaaaattct tcgccatatc ttcgtgaacc
    20401 agtttctgca aatcgacctt ctggctgcgc aatgcgcgga tcaccacatc ggaacataca
    20461 ccgcgttctt gcggaacatc accgccagga taggtaagct gcatatacgc cggatcgtaa
    20521 aatagcgtgc tgccaatttg ctgtctggca ccgtctgcga tggcaaggtt ggtattggcc
    20581 tggatttgta ccacggttgg tggaacggcg ggagatttta aggagtggct ggtaaatgcc
    20641 gttagcaggc tgagcagcgc cagtgaagct ttcatctggt tcgtccgtga aaataagagt
    20701 cacagattcg accttccggg ctaacgattg ttctgtggag aaaatgtcca gaagcggata
    20761 aaaaactcct gaacaacgtg ggagtttcag attactggca cagagtgccg gatgcggagt
    20821 gaacgcctta tccggcctac aaaagcacgc aaattcaata tattgcagag attgcgtagg
    20881 cctgataagc gtagcgcatc aggcaatttt gcgttcaaac tcccggacaa gccgggagtt
    20941 tggagtaggt tattcccact cttgcaggaa acgctgaccg tactggtcag ctaccagcag
    21001 tgcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg ttgtggatgg tttcaccttc
    21061 tgcacaagat gcttttgcaa caatgcgcat tttcgccggg acatcttctt taatatccag
    21121 ttgagcgaga gttattggca aacctaccgc atggctaagt gcagctacgg tttcgatttc
    21181 ctccaccggc gcgttttcca gtaccagctg cgtcagcgta ccaaatgcca ctttttcacc
    21241 gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg ttatgcaccg cgtgagccgc
    21301 cgctttcaaa accaacgccg ctcaaatagg tgttcgcttc aatcacgcgc tccagcgccg
    21361 gggttactac atgctgttcg gcggcaagca tcgctttttc gccttcttcc agcagggtgt
    21421 tgtagcacag ttcagccagt gccagcgccg cctgggtgca cttgccgccc gccatggtgg
    21481 tcgcgccgct acgagagcaa gcacgcgctt caaaccaggt tgccagcgca tcgccgatac
    21541 ccgctgctaa cagacgtgca ggtgcgcccg cgacgatttt ggtgtcgaca atgaccatat
    21601 tcgggttatt tggcaacagc agatagcggt caaactcacc ctcatcggtg tagataacag
    21661 acaatgcgct gcacggtgca tcggtagagg cgatagtcgg tgcgatcgct accggaacac
    21721 ccatgaaatg tgccagtgct ttggcagtat cgagggtttt tccgccaccg ataccgagaa
    21781 ttgcgccaca ctgcgcagtc tccgcgatgc cacgcagacg gtcgatctca ttttgcgaac
    21841 attcaccgcc aaacggcgta atttctacta ccagtccagc atctttaaag cttttctcga
    21901 cagtggattg agcaaaacct aaaacaaatt tgtcacccac cactaaccag cgttctgcca
    21961 gcggcttcag gtattcgccc agacgattaa tcacatcagc gccctggatg tatttacccg
    22021 gtgattgaat aatgcggtcc ataattgctc ctttagagat gagtagtgcc aaatgcggca
    22081 ttccagtcgt gttcgaactt ctctatagct gactctactg caggggtgtt gagcatttgt
    22141 tgcgctacat ctaagggcag ggtgatggat tcacatcctg ccagtaaaca gtccagcgcc
    22201 tgacacggcg ttttaaagct ggctgccagc accatgcttt ctggcgcgtg catttctaac
    22261 agcgtttgca gctcctgaac cgtacgaatg ccgtccccgc cctgggcatc tacgcggtta
    22321 acatacggag caacgtattt tgcccctgcc agtgcggcta ataacccttc tgcggcgcta
    22381 tatacagcgg tgccaagtgt agtaataccc tcttttttca gtattttaat tgctgccaga
    22441 ccttcggaag tcaccgggat tttcaccaca atacccggaa tagcgtcgcg caggcgcttc
    22501 gcttcttcca ccatcccctg cgcgtcgcgg ctcatggtct gagcaaacag aatgccctca
    22561 tcaccaatcg ctttttgcag acgcggcagc acttcccaga tggactcctt gctggcagcg
    22621 ataatgctcg ggttagttgt caccccggca atcgggaata tgcgtgccag acgttcgact
    22681 tctgcgacgt tagcggtgtc cagatacagt tccatgatgt ttccttttac agttccagtt
    22741 catgttgcag caggctggcg atagcgtcgg cagaggcggc gttaaccagc gcgttacgga
    22801 attcttcgtg catgatgcgg cgagcgaggc gcgagaaaat gcgcatgtgc tgatcgcccg
    22861 cagcgtgttt gttcagggtt aacataatga tgaattgcgc ttcatcatcg ccccagcgca
    22921 ccggcgcttg cagacgcgcc acgctgatgg tggattgctc aatgtgttct gatttgctgt
    22981 gtggaatggc aaaactaaag cccagaccgg tagagaaaac ggcctcgcgc gcccataagt
    23041 cggcttccag tttacgcgga tagcggcagc ggcccgccag cagcaggtta tcggtcatgc
    23101 ctttgagcac ttcttctttg ctgcgccagt cactttccag cgtgatgcac tcggcggtga
    23161 ccagcggtgc gtcctgttgg gtcatgcgga attgcgccag caggtgttcc acttccagcg
    23221 aagtacggca ggccattgcc tggttgagca acttgcggca ctcacggcta tcaagttgcg
    23281 ccatccgcgc tttcgccgcc ggaattgatg gtgcgctcat gctgagttca tccagcccta
    23341 agccgaccag caacggcagc acggaacctt tcgctcccag ctcaccgcac agaccaatcc
    23401 atttaccctg gcgatgcacc gcctgcacgg cgtaatcgag cgcccgcaag aatgccggat
    23461 tcaggctgtt gtagtgacga gtaaccttag cgttatcgcg atccactgcc agcagatatt
    23521 gcgtcaggtc attactacca atactaaaga aatcaatctc ttcgcagcat tgatcgatga
    23581 tgaacatcac cgacggcact tccagcataa tgccgagctg gatcttctca tcaaacggaa
    23641 tgtgttcgtt acgtagttgc tgtttggctt ctgccagttt ttctttcacc cataagatct
    23701 cttccattga ggagatcatc gggatcatga ttttcaggct gccgtgagcg gaggcgcgca
    23761 ggatcgagcg tagttgtgtg gtaaataacg acgcgtattc ttcataaata cgcacggcgc
    23821 gataaccgag gaacgggttt gcctctgcgg gaatgtttag ataatcaacg ggtttgtcgc
    23881 cgccaatgtc catagtgcgc acaataatgc tgcgtccgtt ggcagactcc agcgcctgac
    23941 aaaaaatgtt gtacagctcg ctttcgcccg gtgcactggt gcgatccata tagagcattt
    24001 cagtgcggaa caaaccaacg ccttccgcac cattgccgaa tgcggcctgc gcttccacgg
    24061 agtgagcgat gttagcggca atttcaatgc ggataccgtc agcggtacgg gcttgttggg
    24121 tcagccagac acgctgttgc tcacgcaggg cgtcctgtac gcgggcttct tgctgataat
    24181 aacgagctac ggcttctctt ggctcaacca caatcgcccc ggcgttgccg tcgatataaa
    24241 tcgtttgatg ctgccacgga gtgagggcat caatatccac accaaccagc gtcggaatgt
    24301 tgaacgaacg ggcaaggatc accgtatgtg aggtggtgcc gccgcttttc agtaacaatc
    24361 ctttgaggtg atttttatcc agttcgagga actggctggg ggtcagttca tcagccatac
    24421 aaatggcgga ctgcgtcagt ttgcccggtg ccgggaagcg ttgctcaccg tagatttgct
    24481 ggagtaactg gaagcagacg tcgcgtacgt ccagggcacg ttcttgcagg tatctgctgc
    24541 tggaacgggc gaactcttcg caaaagtgat tcgcactggc gacaatcgct tcggcgcagc
    24601 ttaatccggc gctgactcct gccagtaaat gttcgtgcag ggaagtatcg ccagccaggg
    24661 atcggtgcgc ttccagaatc gcgctggtag caccgtcgct atccagcaga cgaaactcaa
    24721 tgtttttcag taccagcgtc aggccgtttt ccagtgcgga ttgctcggcg tcaacgtctt
    24781 tggctgcggg aagattactc agcgcattga gatctaaaga agagatcggc gtcagaatgc
    24841 cgcctgcact accgctgcac acggtgcgga cgcggataat ttgcggattc agattggtca
    24901 gtgaaaccgg cagtggttcc agttcgtcag atttaacttc cgccagcggc gcatcgcagt
    24961 gggggaattc atcgcgcagc cattggctta aacgctggtg cccttcctgt tcgtcggccc
    25021 cggaaatcag tagctggcag ttatcaccca ccagcgtatc ggtgccaatc agcgccagag
    25081 cgcttttggc gttgccttta cggtcagtac gcaggttatg ccactcaatt tgagatgaaa
    25141 aagtattaca cagcgtttca acgtggcttg ccggacgcgc atgtacgccg ttaggtagct
    25201 cacaaataaa ttccacaatc agggccattg cctttctccc ggtacggttt tctgtttaca
    25261 ggataagagc gcacggtaac ggcctgccat gtgacaaatc tgccaaaagc tggacaaatg
    25321 taatgtaacc gtcaatttgc gacgcgtctc acaagacgcc gttttgcggc atgcttccgg
    25381 attatcgcaa gttatgaggc ggatcgcatt tttgtactga tattacaaaa gtccagtaaa
    25441 tggccttttt atccactgtt tgctccgcct gcgattgcct attgtttcct ccatcaaaaa
    25501 tatggacatg ggcttttgca agcccgggag cagcttatga atgagttggt gcagatcctg
    25561 aaaaataccc gtcagcattt aatgacgggc gtttcacaca tgattccctt cgtggtatcg
    25621 ggcggtattt tgctggcggt ttccgtcatg ttgtatggca aaggcgcagt gccggatgcc
    25681 gtagccgatc cgaatttgaa aaaactgttt gatattggcg ttgcaggttt gacgctgatg
    25741 gtgcctttcc tcgccgctta cattggttac tccattgcag agcgttctgc gctggctccg
    25801 tgcgctatcg gtgcctgggt tggtaacagc tttggtgcgg gcttctttgg tgcgctgatc
    25861 gccgggatta tcggcggcat cgtggtgcat tacctgaaga aaattccggt gcataaagtt
    25921 ctgcgctcgg tgatgcccat cttcattatt cctatcatcg gcacactgat taccgcaggc
    25981 atcatgatgt ggggcttggg cgagcctgta ggggcgttga ccaacagcct gactcagtgg
    26041 cttcagggga tgcagcaggg cagcattgtt atgctggcgg tgatcatggg gctgatgctg
    26101 gcgttcgata tgggcggtcc ggttaacaaa gtggcctatg ccttcatgct gatttgcgtt
    26161 gctcagggtg tttataccgt ggtggccatt gctgccgtgg gtatttgtat cccgccgctg
    26221 gggatggggc tggcgacgct gattggtcgt aaaaatttct ccgcagaaga gcgcgaaacc
    26281 ggcaaagcgg cactggtgat gggctgtgtt ggcgttactg aaggggcgat tcctttcgcc
    26341 gctgccgatc cgctgcgtgt cattccttcc attatggtcg gttcagtttg tggtgcagta
    26401 actgcggcgc tggtcggtgc gcagtgctat gcaggctggg gtggtctgat tgtgctgccg
    26461 gtggttgaag gtaagttggg ttatatcgca gcagtggctg tcggagcagt ggtgacggct
    26521 gtttgtgtga acgtgctgaa aagtctggcg cgtaaaaatg ggtcttcgac tgatgaaaaa
    26581 gaagacgacc tggatttgga ttttgaaatt aattaattga ggaacgagcc atgacgaaaa
    26641 ttattgcagt aaccgcatgt ccttcaggtg ttgcccatac ctatatggta gcagaggcgc
    26701 tggaaagtgc cgcgaaagcg aaaggctggg aagtgaaagt agaaacccag gggtcaattg
    26761 gtctggaaaa tgaactgact gcggaagacg tggcgagcgc tgatatggtg attctgacca
    26821 aagatatcgg catcaagttt gaagagcgtt ttgctggcaa gaccatcgtg cgcgtcaaca
    26881 tcagcgatgc agtaaaacgt gctgacgcca ttatgagcaa aattgaagct cacctggcgc
    26941 aaaccgctta agttcttctc ccgctcgcaa gggcgggttc gctttctcac aggagttcct
    27001 catgacgaat cgtatctctc gcctcaaaac tgcactgttt gccaataccc gtgaaatctc
    27061 gctggagcgg gcgctgcttt ataccgccag ccatcggcaa accgaaggcg aaccggtgat
    27121 attgcgccgg gcgaaagcaa cagcgtatat ccttgaacat gttgaaattt cgattcgtga
    27181 tgaagaactg attgccggta accgcaccgt aaaaccgcgc gccgggatta tgtcgccgga
    27241 aatggaccct tactggctgc tgaaagagct ggatcaattc ccgacgcgtc cgcaggaccg
    27301 ctttgctatc agcgaagaag ataaacgtat ctaccgtgaa gagttgttcc cgtactggga
    27361 aaaacgttcg atgaaagatt tcatcaacgg gcagatgaca gatgaagtaa aagccgcgac
    27421 caacacgcag attttcagca tcaaccagac ggataaaggc caggggcaca ttattattga
    27481 ttacccacgc ctgttgaatc acggtctggg tgagctggtg gcacagatgc agcaacattg
    27541 tcagcaacag ccggagaatc acttttatca ggccgcgttg ttactgctgg aagcctcgca
    27601 gaaacacatt ttgcgttacg ccgaactggc ggaaacgatg gcggcaaact gcacagatgc
    27661 ccagcgtcgc gaagagctgc tgactattgc ggagatctcc cgccataacg cgcaacataa
    27721 gccgcagacg ttctggcagg cgtgccagtt attctggtac atgaacatca ttctgcaata
    27781 cgaatccaac gccagttcgc tgtctttagg gcgcttcgac cagtatatgt tgccgttcta
    27841 tcaggcatct ttaacccagg gcgaagatcc ggcgttcctg aaagaactgc tcgaatcttt
    27901 atgggtgaaa tgcaacgaca tcgtgctgtt gcgctccacc agtagcgcgc gttatttcgc
    27961 cggtttcccg accggctata ccgcactgct cggcgggtta accgagaacg gacgtagcgc
    28021 ggtgaacgtg ctttcgttcc tttgccttga cgcctatcaa agcgtgcaat taccgcaacc
    28081 gaacctcggc gtgcgcacta acgccttgat cgacacgccg ttcctgatga aaaccgccga
    28141 aaccattcgc ctcggcaccg gtattccgca aatctttaac gatgaagtgg tggtgccagc
    28201 gttcctcaac cgtggcgttt cgctggaaga tgcgcgcgac tattccgtag tgggctgtgt
    28261 ggaattatct attcccggca gaacctacgg cttgcatgac atcgcgatgt ttaatctgct
    28321 gaaagtgatg gaaatctgcc tgcatgaaaa tgaaggcaat gctgcgctga cttatgaagg
    28381 tttactggag cagatccgcg ccaagatcag ccactacatc accctgatgg ttgagggcag
    28441 caatatttgt gatatcggcc atcgcgactg ggcacctgta ccgctgctct cgtcttttat
    28501 cagcgattgt ctggaaaaag gtcgcgatat taccgatggc ggcgcgcgtt ataacttctc
    28561 cggcgtacag ggaattggta tcgccaacct gagcgattct ctccatgcgt tgaaagggat
    28621 ggtttttgag caacagcgtt taagttttga cgaattgctg tcggtattaa aagccaactt
    28681 tgcaacgcca gaaggcgaaa aagtccgcgc tcgcttaatt aaccgctttg agaaatacgg
    28741 taacgatatc gacgaggtgg ataacattag cgccgaactg ttgcgccact actgcaaaga
    28801 agtggaaaaa taccagaacc cgcgcggcgg ttacttcacg ccgggatcgt ataccgtttc
    28861 tgctcacgtt ccgttgggat cggtggttgg cgcgacgcca gacggtcgtt ttgccgggga
    28921 acaactggca gacggcggct tgtcacctat gctgggtcag gacgcacaag ggccaacggc
    28981 ggtactgaag tcagtcagta agctcgataa tacgctgctg tctaacggta cgttgctgaa
    29041 cgtgaaattc actccggcga ccctggaagg tgaagcggga ttacgcaaac tggccgactt
    29101 cttacgggtg tttacccagc ttaagttgca gcatattcag tttaacgtgg tgaacgccga
    29161 cacgttgcgg gaagcgcaac agcgcccaca agattatgcc gggctggtgg tgcgcgttgc
    29221 tggatacagc gccttctttg tcgaactgtc gaaggagatc caggatgaca tcatccgccg
    29281 gacagcgcat cagctgtaac gttgtggaaa cgcgccgcga tgatgtggcg cgcattttca
    29341 acattcagcg ttattcactg aatgacggcg agggcattcg tacggtggtc ttttttaaag
    29401 gctgtccgca tctttgcccg tggtgtgcta atccggagtc gatctccggc aaaatccaga
    29461 cggtacgcag agaggcgaaa tgtctgcact gtgcgaaatg tttgcgtgat gcggatgaat
    29521 gcccctccgg ggcgtttgaa cggattggtc gcgatatcag ccttgacgct ctggaacggg
    29581 aagtgatgaa agatgacatt ttctttcgca cgtccggcgg cggcgtcacg ctttctggcg
    29641 gcgaagtgtt aatgcaggcg gagtttgcta cccgtttttt acagcgactg cggctgtggg
    29701 gtgtctcatg cgccattgaa actgccggag acgcgccagc aagcaagctg ttaccgctgg
    29761 cgaaattgtg cgatgaagtg ctgttcgatt taaaaattat ggacgcgact caggcgcggg
    29821 atgtggtgaa gatgaacctg ccacgcgtgc tggagaatct gcgtttgctg gtgagtgagg
    29881 gtgtcaacgt gatcccgcgt ttaccgttga tccctggttt cacgctcagc cgggagaata
    29941 tgcagcaggc gctggatgta ctgatcccgc tgaaaatcag gcagatccat ctgttaccgt
    30001 ttcatcagta cggcgaaccg aaataccgcc tgctggggaa aacatggtcg atgaaagagg
    30061 tgcctgcgcc gtcgtcagcc gatgtgacaa cgatgcgcga aatggcagaa cgggccggat
    30121 ttcaggttac cgtgggaggt taaaatggca tacctggtgg cagtaaccgc ctgcgtaagc
    30181 ggcgtggcgc atacttatat ggcggcggaa cggctggaaa agttgtgcca gttagagaag
    30241 tggggcgtca gcattgaaac tcagggcgcg ctgggaacgg agaatcgttt agcggacgag
    30301 gatatccgtc gggcggatgt tgctctgttg attacggata tcgagcttgc cggtgccgag
    30361 cgatttgaac attgccgcta tgtgcaatgc agcatctacg cattcctgcg tgagccgcag
    30421 cgggtaatga gcgcggtgcg caaagtgctt tctgcgccgc agcaaaccca tcttattctg
    30481 gagtagtcgg tttttctgtc agttggctgt ggtactgccg gcgatattcc gacggcgagt
    30541 gttcggtgtt tttacgaaac agacggcaga agtagttgct gtcgacaaag ccgcaggcgt
    30601 gcgccacttc ttttaccttc aggtcatagc ctttcaataa cgtcttagcg tgctccagtc
    30661 gcgtgtgatt caggtattcg ttaaaaccaa tggcccccgt tttttgaaac aggtgcgaga
    30721 gatagtttgg tgaaatatag aatgcctgtg cgacagattc gcgggtaagc gcggaggcat
    30781 agcgttcgtc gatataatcg cgaatagctt caaatagtgc ctggctgcgt gaggcggtct
    30841 ggatttggct gccgcgcaaa tcgcggcagt ggctaagcag gctggctacg ataagccttg
    30901 ccgtttgctg ctcctgcggc tgcatttgca tttcattgag cgtttgtagc agaaaagaac
    30961 ctatgcgtgg gccgcgtcgg gcgacgtgtt gcttcgccag attttgatat tgtttgccat
    31021 cccattgcac gacgctgaag ccgagttgct gtttgccaaa cagcacgcta aaggtggtag
    31081 cgggtgtttt ccattgtggg aaattccagc caccagcggc aacgtacagc acatcgccgg
    31141 gaactaacgt tgctccagcg ccggtatcaa caaactcacc ttccagcaca atttccagcc
    31201 gtggaaaatc gacctgatac gcaagatcgg gcaccgggcc gttactgctg gcaaagtaaa
    31261 tctggcgcag ggacagcggg ccgttgatca ggcgggagag caaatagctg acgtcgtgat
    31321 acatgttatt cccttaggta tgtagacggg aaaacagtaa ctggcattat taaaaaaggc
    31381 cacctgaaaa gtgtggcctg aggggagttc gatgtaaaca ttgcggcagt atcgcatccg
    31441 gcaaatagcg ccagacgcta tacaaaggtc atctactgat tacccacctg atcgccatac
    31501 ggcagagtgt cgtaatcgat cagcgcgttt ttcttgtacg ggttaccaat ccagcgggta
    31561 gtttctttga actgcggatt caccactgaa cgggttggat cgtaaccgtc gtaagataag
    31621 cccgccaaat ctgaccaggt gtggatcagt tccgccaggc tgtatttacg atcgacatcc
    31681 tgtgagaaat cacggggatg agtcgcttgc catttttccg acgtccacag caggaacgga
    31741 atggtgtaca tgtggcgcgt cgggttgtct tcgttacgcc cctgagtttt gtgcggcggc
    31801 gtgtcgtaaa cctcttcacc gtggtcagag aagtaaacca ggaatccgtt cgggtctgcc
    31861 gctttaaagt ctttaatcag gctggcaacc acatgatcgt tatacaggtt agcgttgtca
    31921 taatcgttat atgattccag ctcttctgcg cttaatcctg gaggaacatg atcggtattg
    31981 ccatcaaact tgccctgatt ttccgggtag cggtatttgt atttgatatg cgtacccagc
    32041 agatgaacga tgatcagttt cttcggcgca gggtcattca gcacatcctg gaacggcttc
    32101 agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt gctggttcat gtagtactgc
    32161 ttgtcggtct ggcgcgaaaa taccgtcagc atggtattgc gggcggtcat cgtctgctgg
    32221 ttggtgatcc agaaggtttt ataacccgcc tgtttcatca tgttcatcag cgacggctgc
    32281 gtcagataca gatccgggtt cttttcattg gcaaaggtca gcgcctgttg caggatttca
    32341 atggtgtacg gacgagacgt aactacgtta ttaaacacgg tcagattcgg atcggtttta
    32401 tgcagcgcat ccagctccgg cgtggtttca cgcggataac cgtacagact catgcgtccg
    32461 cgctgggtcg actcgccaat caccagcact aaagtgcgcg gttcgttacc cgattcatct
    32521 ttgaaattag ccagtggcgg caaggcatta ttttcattca gtaactttgt tagcgagttt
    32581 agttgctgac gatactgata atagccggtc aggaattgcc acggtgcggc aggctccata
    32641 cgcgaggcta ggttatccaa cgttttctca aacggcttgt ttttgataaa cgtattcatg
    32701 gcgatcggat gcagaatcaa gccataaagc agggcaaaag agacaacata acgccacggc
    32761 tttggaatat agaccgggcg caggcgtgtc cacagcagaa ctgccaccgc cgtataggcc
    32821 agcgcgataa gcacaatttt caggctgaaa tactggctta aatactcgct ggcttcgttg
    32881 gtgttggttt cgaacatcac aaacagaacg ctctgcgaga actcctgccc gtagatgacg
    32941 tagtagcaca gcgccgccag agaggccgtc catagcacca cgccgatcac tgcggcaata
    33001 attttaatcc gcttcggaaa gaggaatacc gggatcaacc acagcgagct gaataacagc
    33061 gagtcgcgaa tgccgttggt gccactataa ccactgatgt aaataatggc ctgtagcaga
    33121 gtagagaaaa accaaaagta gagcagtgcc caacccaggg ctttccagct agaaaggggt
    33181 ttagcctgga cttctgtgga atgcatagta agaacctgtc ttgaaaaaaa tatcgccgaa
    33241 tgtaacgaca attccttaag gatatctgaa ggtatattca gaatttgaat aaaatgcaga
    33301 cagaaatata ttgaaaacga gggtgttaga acagaagtat ttcagaaaac cctcgcgcaa
    33361 aagcacgagg gtttgcagaa gaggaagatt agccggtatt acgcatacct gccgcaatcc
    33421 cggcaatagt gaccattaac gcttgttcga cgcgaggatc cggttcctgg ccttcttttt
    33481 ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac gttcagcggg tcggtgtaaa
    33541 tattccgtag ctgaatagac tctgcaatcc acggcagatc ggccatcaga tgggaatcgt
    33601 tggcaatcgc cagcaccact ttgatgtctt cttcttgcag gttgcgtaac tctttaccta
    33661 acggccacag tgctttgtct accagacgtt gatcatagta ttccgccaac cacaggtctg
    33721 ctttggcgaa gaccatctcc agcatgccga gacgcgtcga gaagaatggc caatcgcggc
    33781 acatggcttc cagctcgctc tgtttgccat cttcgaccac tttttgcagc gccgtacctg
    33841 cacccagcca ggcggggagc atcaggcggt tttgcgtcca ggcaaaaatc cacggtatgg
    33901 cgcgcagcga ctcgacgccg ccggttgggc gacgtttcgc cggacgtgaa cccaacggca
    33961 gtttgcccaa ttcttgttcc ggcgtagcgg agcggaagta aggcacaaaa tctttgtttt
    34021 cacgtacgta gccacggtag agatcgcagg agatgactga cagttcatcc ataatgcgac
    34081 gccagctctc tttcggctca ggtggtggca gcaggttggc ttccagaatc gccccggtgt
    34141 aaagcgacag gctgctgacg gtgatttctg gcagaccata tttaaagcgg atcatctcgc
    34201 cctgttcagt cacgcgcagg ccacctttca ggcttcctgg cggttgtgac agcagcgccg
    34261 catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg accgtggaac aacgtcagct
    34321 caatacccgc tttttcgcag gttttgatta atgcatcctg tgcctgatat tgcgcccagg
    34381 aagctgccat cacgcccgca tcttttgctg agtcggaata gccaatcatc accatctgtt
    34441 tgccctgaat caggccgcga taccagtcga tattgagcag ctgggtcatg acatcgttgg
    34501 cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac cggcatcgca aacccgatac
    34561 ccgcttcttt cagcagcagg tggacagcca gtacgtcgga cggcgttttc gccatcgaga
    34621 tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat cacctggcag gtatcgagca
    34681 cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggcag aagcggacgt ttggagttca
    34741 gttcgcggat caggaacgcc tgtttgtcgg cctctgacca gctttcgtag tcgccgatac
    34801 cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg ggtgctctcc tgacggatat
    34861 caatacggac cagcggtacg ccgaaacatt tcacgcggcg cagggtgtcg agcagatcgc
    34921 cgttggcgat aatacccatg ccacacgcct gaagtgactg gtagcaagcg tagagcggtt
    34981 cccacagttc ttcgttttgt gtcagcagcc cttctggttt tggcagttct tcgcctttca
    35041 ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga acgcaggttt ttcatcagat
    35101 agcgatacgg ttctgcggca ccttcttcgc caaccagcgc cagcagttca ggggtcgctt
    35161 caaccatcga cagttcagaa accagaacct gaatatcttt caggaacaga tcggtggctt
    35221 tccagcggct gagcagcagg acgtggcggg tgatatcggc agtgacgttc gggttgccgt
    35281 cgcggtcacc gcccatccac gaggtaaaac gtaccggaac aaattcgacg ggcagtttgt
    35341 agtcgagatt ctcttccagt tgttcgttca gttcgcgcag gtaattcggt acgccttgcc
    35401 acaggctgtt ttctactacg gcaaagcccc atttggcttc atctaccggg cttggacgca
    35461 gcttacggat ttcatcggta tgccatgact gggcgatcaa ctggtgcagg cgacgcatca
    35521 gctggttgtg ttcgtagtca gcgatatctt tgttatcgag ctgttttaaa caggcgttca
    35581 cttccaccat tttgtggatc agtgtacgac gggtaatttc ggttgggtga gccgtgagga
    35641 ccagttccag cgacagcgat tccactgctt ttttgatggt gtcttcgctc agttccggct
    35701 ggtttttcag tttacgcagg gtgcgggcga tcacttccgg gttgctggca gcttcgcctt
    35761 tcggcgaaat gctgtggtat tgctcggcgg tgttggccag gttcaggaac tgactaaacg
    35821 cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg taaggtggtg agcaactcct
    35881 ggcggttagc atcattgcca gcgcgtgaag atttcgacaa cttacggata gtttctacgc
    35941 gttcaagaat gtgttctccc aacgcatcct tgatggtttc tcccagcact ttgccgagca
    36001 tactgacatt actacgcaat gcggaatatt gttcgttcat attaccccag acaccccatc
    36061 ttatcgtttg atagccctgt atccttcacg tcgcattggc gcgaatatgc tcgggctttg
    36121 cttttcgtcg tcttttataa agccacgtaa aagcggtgac gtcaaatgct gcgaaatcgc
    36181 ttcagcaaac gaataaatag caggaattta cgccattaaa ttcacgacgc tttaaataag
    36241 cgtaacttat ggaaatgtta aaaaatcgcc atacataaca ccaaaggtgt aggtcggata
    36301 agatgcgcaa gcatcgcatc cgacattatt gcggcaccgg agtttggcaa cggtgccgga
    36361 tgcggcgcga gcgccttatc cggcctacgt tttaatgcca gcaaaaatgg tgaattacct
    36421 ggataatcaa ctcacgggtg ggtttaataa accgcgtctc cagatattca tcaggctgat
    36481 gagcttgatt gatggaaccc ggaccgagca ccagcgtcgg gcataacgtt tgaataaacg
    36541 gcgcttcggt acagtaattc accacttcgg tttttgctcc gagcaatttc tcaaccactt
    36601 caaccagttg atgattcggt gggcattcat agccagggat cggcggatgc agctcgtcga
    36661 ccgtcagacg acccggccag cgttcgctca ccggagccaa tgcatcgttg agcaaaccat
    36721 taagttcatt gagtgtcatg ccaggctgcg gacgaatatc catatgcaac tcacagcaag
    36781 cgcaaatacg gttagaagcg tcgccaccgt gaatatgccc gaggttgagc gtagggtatg
    36841 gcacggtaaa tgcttcgtag tgataacgtt ctttcaggtt atcgcgcaat tgcaaaatat
    36901 gcccgatggc gtcgtgcatt agttcgatag cgttaactcc gcgtgctgga tcgctggagt
    36961 gccccgactg gccctgaata cggatggcgt tagagatatg acctttatgt gcgcgtaccg
    37021 gttgtagtga cgtcggttcg ccaatgatgg cgcaatccgg gcgcagggcg gtagtttcgg
    37081 caaaataacg cgctccggcc atactggttt cttcatcagc agtcgccaga atgtagagcg
    37141 gttttttcag tttcgtgacg tcgacatcgc gtagcgcatc aaggataaac gcaaaaaagc
    37201 ctttcatgtc ggcggtgcct aagccgtaaa gcttgccgtc atgctccgtc agtgtaaacg
    37261 gatcgcgcgt ccagcgaccg tcatcaaatg gcaccgtatc ggtatgcccc gccagcaaca
    37321 agccgccagc cccctgtccg taactggcca gcatattaaa tttgttgcga gttcctggaa
    37381 caggctgcac ttccacattg aagcccaaat ctttaaacca gtccgccagc agagtgatta
    37441 aatctgcatt gctttgatcg agtgcctctt ccgtagcgct tattgaaggt gtggcaatca
    37501 gagcgcggta aatctcgata aatggcggta atttgttttt cattgttgac acacctctgg
    37561 tcatgatagt atcaatattc atgcagtatt tatgaataaa aatacactaa cgttgagcgt
    37621 aataaaaccc accagccgta aggtgaatgt tttacgttta acctggcaac cagacataag
    37681 aaggtgaata gccccgatgt tgaatacgct gattgtgggt gccagcggct acgctggcgc
    37741 agagctagtg acctatgtaa atcgccatcc gcatatgaac ataaccgctt tgactgtttc
    37801 agcgcaaagc aatgatgcgg gaaagttaat ctccgatttg catccgcagc taaaaggcat
    37861 cgttgagctg ccgttgcagc cgatgtcgga tatcagcgag tttagcccag gggtggacgt
    37921 agtgtttctc gccaccgccc atgaagttag ccacgattta gcgccgcagt ttcttgaagc
    37981 gggctgcgtg gtgttcgacc tttccggcgc gtttcgtgtt aacgacgcca ccttctatga
    38041 aaaatattac ggctttaccc atcaataccc ggaactgctg gaacaggcag cctacggtct
    38101 ggcggagtgg tgcggtaata aattaaaaga agcgaatttg attgcggtgc caggctgtta
    38161 tccgacggcg gcacagctgg cgctgaaacc gttgattgat gccgatcttc ttgacctcaa
    38221 tcagtggccg gtgatcaacg ccaccagcgg cgtgagcggt gcaggacgta aggcagcgat
    38281 ttcaaatagt ttttgtgaag ttagcctgca accgtatggc gtctttactc atcgccatca
    38341 accagagatc gccacacacc tcggtgctga cgttatcttc accccacatc tgggcaattt
    38401 cccgcgcggc attctcgaaa ccattacctg ccgcctgaaa tcgggtgtga cccaggcgca
    38461 agtcgcgcaa gcgttacagc aggcgtatgc ccataaaccg ctggtgcggc tgtatgacaa
    38521 aggcgttccg gcgctgaaaa atgtcgttgg gctgccattt tgcgatatcg ggtttgccgt
    38581 tcagggcgag catctgatta ttgtggcgac cgaagacaac ttactgaaag gcgcggcggc
    38641 acaagcggta cagtgcgcca atattcgttt cggctatgcg gaaacgcagt ctcttattta
    38701 agggtgcaat gatgaatcca ttaattatca aactgggcgg cgtactgctg gatagtgaag
    38761 aggcgctgga acgtctgttt agcgcattgg tgaattatcg tgagtcacat cagcgtccgc
    38821 tggtgattgt gcacggcggc ggttgcgtgg tggatgagct gatgaaaggg ctgaatctgc
    38881 cggtgaaaaa gaaaaacggc ctgcgggtga cgcctgctga tcagatagac attatcaccg
    38941 gagcactggc gggaacggca aataaaaccc tgttggcatg ggcgaagaaa catcagattg
    39001 cggccgtagg tttgtttctc ggtgacggcg acagcgtcaa agtgacccag cttgatgaag
    39061 agttaggtca tgttggactg gcgcagccag gttcgcctaa gcttatcaac tccttgctgg
    39121 agaacggtta tctgccggtg gtcagctcca ttggcgtaac agacgaaggg caactgatga
    39181 acgtcaatgc cgaccaggcg gcaacggcgc tggcggcaac gctgggcgcg gatctgattt
    39241 tgctctccga cgtcagcggc attctcgacg gcaaagggca acgcattgcc gaaatgaccg
    39301 ccgcgaaagc agaacaactg attgagcagg gcattattac tgacggcatg atagtgaaag
    39361 tgaacgcggc gctggatgcg gcccgcacgc tgggccgtcc ggtagatatc gcttcttggc
    39421 gttatgcgga gcagcttccg gcactgttta acggtatgcc gatgggtacg cggattttag
    39481 cttaagtttt gttagccgga ggagcagctt tccggcattg aatttcaaaa taaggaaaca
    39541 gagttatggc actttggggc gggcgtttta cccaggcagc agatcaacgg ttcaaacaat
    39601 tcaacgactc actgcgcttt gattaccgtc tggcggagca ggatattgtt ggctctgtgg
    39661 cctggtccaa agccttggta acggtcggcg tgttaaccgc agaagagcag gcgcaactgg
    39721 aagaggcgct gaacgtgttg ctggaagatg ttcgcgccag gccacaacaa atccttgaaa
    39781 gcgacgccga agatatccat agctgggtgg aaggcaaact gatcgacaaa gtgggccagt
    39841 taggcaaaaa gctgcatacc gggcgtagcc gtaatgatca ggtagcgact gacctgaaac
    39901 tgtggtgcaa agataccgtt agcgagttac tgacggctaa ccggcagctg caatcggcgc
    39961 tggtggaaac cgcacaaaac aatcaggacg cggtaatgcc aggttacact cacctgcaac
    40021 gcgcccagcc ggtgacgttc gcgcactggt gcctggccta tgttgagatg ctggcgcgtg
    40081 atgaaagccg tttgcaggat gcgcttaagc gtctggatgt cagcccatta ggctgtggcg
    40141 cgctggcggg aacggcctat gaaatcgacc gtgaacagtt agcaggctgg ctgggctttg
    40201 cttcggcgac ccgtaacagt ctggacagtg tttctgaccg tgaccatgtg ttggaactgc
    40261 tttctgctgc cgctatcggc atggtgcatc tgtcgcgttt tgctgaagat ctgattttct
    40321 ttaacaccgg cgaagcgggg tttgtggagc tttcggaccg cgtgacttcc ggttcatcat
    40381 taatgccgca gaagaaaaac ccggatgcgc tggagctgat tcgcggtaaa tgcggccggg
    40441 tgcagggtgc gttaaccggc atgatgatga cgctgaaagg tttgccgctg gcttacaaca
    40501 aagatatgca ggaagacaaa gaaggtctgt tcgacgcgct cgatacctgg ctggactgcc
    40561 tgcatatggc ggcgctggta ctggacggca ttcaggtgaa acgtccgcgt tgtcaggaag
    40621 cggcgcagca ggggtatgcg aacgccactg aactggcgga ttacctggtg gcgaaaggcg
    40681 tacctttccg cgaggcgcac catattgtgg gtgaagcggt ggtggaagcc attcgtcagg
    40741 gcaaaccgct ggaagatctg ccactcagtg agttgcagaa attcagtcag gtgattggcg
    40801 aagatgtcta tccgattctg tcgctacaat cgtgcctcga aaagcgtgcg gcaaaaggcg
    40861 gcgtctcacc gcagcaggtg gcgcaggcga ttgcttttgc gcaggctcgg ttagagtaat
    40921 ttgtttatat tgctcccctg aaaatgggga gtttaccacg cctacaaagt gcgacacttc
    40981 gtaggcggat aaggcgttta cgccgcatca ggcagaaatt tgtcagcatt ctg
//
LOCUS       DADLTF010000017        40847 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.017, whole genome shotgun sequence.
ACCESSION   DADLTF010000017 DADLTF010000000
VERSION     DADLTF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.017"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(232..1023)
                     /gene="cpoB"
                     /locus_tag="JN749_000889"
     CDS             complement(232..1023)
                     /gene="cpoB"
                     /locus_tag="JN749_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HAY7367388.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1033..1554)
                     /gene="pal"
                     /locus_tag="JN749_000890"
     CDS             complement(1033..1554)
                     /gene="pal"
                     /locus_tag="JN749_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HAY7367389.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(1589..2881)
                     /gene="tolB"
                     /locus_tag="JN749_000891"
     CDS             complement(1589..2881)
                     /gene="tolB"
                     /locus_tag="JN749_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HAY7367390.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3014..4270)
                     /gene="tolA"
                     /locus_tag="JN749_000892"
     CDS             complement(3014..4270)
                     /gene="tolA"
                     /locus_tag="JN749_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HAY7367391.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAK
                     AAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADI
                     NNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAA
                     KLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4335..4763)
                     /gene="tolR"
                     /locus_tag="JN749_000893"
     CDS             complement(4335..4763)
                     /gene="tolR"
                     /locus_tag="JN749_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HAY7367392.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4767..5459)
                     /gene="tolQ"
                     /locus_tag="JN749_000894"
     CDS             complement(4767..5459)
                     /gene="tolQ"
                     /locus_tag="JN749_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HAY7367393.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5456..5860)
                     /gene="ybgC"
                     /locus_tag="JN749_000895"
     CDS             complement(5456..5860)
                     /gene="ybgC"
                     /locus_tag="JN749_000895"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HAY7367394.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6010..6303)
                     /gene="ybgE"
                     /locus_tag="JN749_000896"
     CDS             complement(6010..6303)
                     /gene="ybgE"
                     /locus_tag="JN749_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HAY7367395.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6303..6416)
                     /gene="cydX"
                     /locus_tag="JN749_000897"
     CDS             complement(6303..6416)
                     /gene="cydX"
                     /locus_tag="JN749_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HAY7367396.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6431..7570)
                     /gene="cydB"
                     /locus_tag="JN749_000898"
     CDS             complement(6431..7570)
                     /gene="cydB"
                     /locus_tag="JN749_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HAY7367397.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7586..9154)
                     /gene="cydA"
                     /locus_tag="JN749_000899"
     CDS             complement(7586..9154)
                     /gene="cydA"
                     /locus_tag="JN749_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HAY7367398.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(10003..12636)
                     /gene="mngB"
                     /locus_tag="JN749_000900"
     CDS             complement(10003..12636)
                     /gene="mngB"
                     /locus_tag="JN749_000900"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HAY7367399.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKIWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(12654..14630)
                     /gene="mngA"
                     /locus_tag="JN749_000901"
     CDS             complement(12654..14630)
                     /gene="mngA"
                     /locus_tag="JN749_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HAY7367400.1"
                     /translation="MVLFYRAHWRDYKNDQVRIMMNLTTLTHRDALCLNARFTSREEA
                     IHALTQRLAALGKISSTEQFLKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVAT
                     LSEPLQWESVDGPEAIDLVVLLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATT
                     PDELLSALDDKGGTQPSASFSNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVY
                     VEKQGANGIEGRLTADQLNSATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEAL
                     IQQSLTLERGDETRTVQQDTQPVKSVKTELKQALLSGISFSVPLIVAGGTVLAVAVLL
                     SQIFGLQDLFNEENSWLWMYRKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGL
                     AANMIGSGFLGAVVGGLIAGYLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLML
                     FVVGEPVAWINNSLTAWLNGLSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMA
                     NGVYGPYAIFASVKMVSAFTVTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPM
                     AIEDPLRVIGSFVLGSMVTGAIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWF
                     GAALVGAAISTAILLIWRRHAVKHGNYLTDGVMQ"
     gene            14740..15462
                     /gene="mngR"
                     /locus_tag="JN749_000902"
     CDS             14740..15462
                     /gene="mngR"
                     /locus_tag="JN749_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HAY7367401.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(15566..16435)
                     /gene="sucD"
                     /locus_tag="JN749_000903"
     CDS             complement(15566..16435)
                     /gene="sucD"
                     /locus_tag="JN749_000903"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HAY7367402.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(16435..17601)
                     /gene="sucC"
                     /locus_tag="JN749_000904"
     CDS             complement(16435..17601)
                     /gene="sucC"
                     /locus_tag="JN749_000904"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HAY7367403.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(17695..18912)
                     /gene="odhB"
                     /locus_tag="JN749_000905"
     CDS             complement(17695..18912)
                     /gene="odhB"
                     /locus_tag="JN749_000905"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HAY7367404.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(18927..21728)
                     /gene="sucA"
                     /locus_tag="JN749_000906"
     CDS             complement(18927..21728)
                     /gene="sucA"
                     /locus_tag="JN749_000906"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HAY7367405.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(22029..22745)
                     /locus_tag="JN749_000907"
     CDS             complement(22029..22745)
                     /locus_tag="JN749_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HAY7367406.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(22761..24527)
                     /gene="sdhA"
                     /locus_tag="JN749_000908"
     CDS             complement(22761..24527)
                     /gene="sdhA"
                     /locus_tag="JN749_000908"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HAY7367407.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(24527..24874)
                     /gene="sdhD"
                     /locus_tag="JN749_000909"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(24527..24874)
                     /gene="sdhD"
                     /locus_tag="JN749_000909"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HAY7367408.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(24868..25272)
                     /gene="sdhC"
                     /locus_tag="JN749_000910"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(24868..25272)
                     /gene="sdhC"
                     /locus_tag="JN749_000910"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7367409.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            25653..25760
                     /locus_tag="JN749_000911"
     CDS             25653..25760
                     /locus_tag="JN749_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367410.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            25966..27249
                     /gene="gltA"
                     /locus_tag="JN749_000912"
     CDS             25966..27249
                     /gene="gltA"
                     /locus_tag="JN749_000912"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HAY7367411.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            27640..28206
                     /locus_tag="JN749_000913"
     CDS             27640..28206
                     /locus_tag="JN749_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7367412.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATCVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            28257..30716
                     /locus_tag="JN749_000914"
     CDS             28257..30716
                     /locus_tag="JN749_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7367413.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALALEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGQVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            30731..31462
                     /locus_tag="JN749_000915"
     CDS             30731..31462
                     /locus_tag="JN749_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7367414.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            31459..32521
                     /locus_tag="JN749_000916"
                     /pseudo
     CDS             31459..32521
                     /locus_tag="JN749_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            32628..33719
                     /locus_tag="JN749_000917"
     CDS             32628..33719
                     /locus_tag="JN749_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAY7367415.1"
                     /translation="MLYRYTKEKIMRERRMPVLQWGMLCVLSLLLSIGFLAVHLPAAL
                     LLGPMIAGIIFSMRGITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAI
                     LLVTLLSSAIVGWLLVRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYL
                     RVLFVAGAAVLVTRMMLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLP
                     SGTMLIPMLAGAVLQSGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQ
                     ILLSIFALLAICAGMAWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMA
                     MQTLRLFSILLTGPAIARFISTYAPKRSA"
     gene            complement(33716..34507)
                     /gene="nei"
                     /locus_tag="JN749_000918"
     CDS             complement(33716..34507)
                     /gene="nei"
                     /locus_tag="JN749_000918"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HAY7367416.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(34543..35277)
                     /gene="pxpA"
                     /locus_tag="JN749_000919"
     CDS             complement(34543..35277)
                     /gene="pxpA"
                     /locus_tag="JN749_000919"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HAY7367417.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(35267..36199)
                     /gene="pxpC"
                     /locus_tag="JN749_000920"
     CDS             complement(35267..36199)
                     /gene="pxpC"
                     /locus_tag="JN749_000920"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HAY7367418.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(36193..36849)
                     /gene="pxpB"
                     /locus_tag="JN749_000921"
     CDS             complement(36193..36849)
                     /gene="pxpB"
                     /locus_tag="JN749_000921"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HAY7367419.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQQHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(36872..37615)
                     /gene="ybgI"
                     /locus_tag="JN749_000922"
     CDS             complement(36872..37615)
                     /gene="ybgI"
                     /locus_tag="JN749_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HAY7367420.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            37886..39367
                     /gene="dtpD"
                     /locus_tag="JN749_000923"
     CDS             37886..39367
                     /gene="dtpD"
                     /locus_tag="JN749_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HAY7367421.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(39517..>40847)
                     /gene="phrB"
                     /locus_tag="JN749_000924"
     CDS             complement(39517..>40847)
                     /gene="phrB"
                     /locus_tag="JN749_000924"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HAY7367422.1"
                     /translation="VLALYIATPRQWETHNMSPRQAELINAQLNGLQIALAEKGIPLL
                     FREVDDFVASVEIVKQVCAENSVTHLFYNYQYEVNERARDVDVERALRNVVCEGFDDS
                     VILPPGAVMTGNHEMYKVFTPFKNAWLKRLREGMPECVAAPKVRSSGSIKPAPSITLN
                     YPRQSFDTAHFPVEEKAAIAQLRQFCENGAGEYEQQRDFPAVEGTSRLSASLATGGLS
                     PRQCLHRLLAEQPQALDGGAGSVWLSELIWREFYRHLMTYYPSLCKHRPFIAWTDRVQ
                     WQSNPAHLQAWQKGKTGYPIIDAAMRQLNSTGWMHNRLRMITASFLVKDLLIDWREGE
                     RYFMSQLIDGDLAANNGGWQWAASTGTDAAPYFRIFNPITQGEKFDREGEFIRRWLPE
                     LRDVPGKAVHEPWKWAQKAGVKLDYPQPIVEHKEARVQTLAAYETARKGK"
BASE COUNT         9873 a        11052 c         9895 g        10027 t
ORIGIN      
        1 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
       61 acgacccact ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata
      121 ttactgattt cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg
      181 attcgcacga cacgaccaga aataatgcga cttctggtcg tgttttatgc attacatcgc
      241 gttcagacgt ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg
      301 ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt
      361 aaacatcgca tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata
      421 gtacgccgca tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc
      481 gtttggcagg taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc
      541 gtcatcctgg cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc
      601 atcgccgctt ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt
      661 tgatgccgcc gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct
      721 gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata
      781 ctgattttcc tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg
      841 ttgctggagt tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac
      901 gcggtcttcg accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca
      961 gggggccgct ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct
     1021 catgcaattc tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ctgcttcgtc
     1081 atgacccagt actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga
     1141 aacgccttta ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat
     1201 gttgtattcc ggagtaccac gttcgtccgc gtgaccttct acggtgactt tgtaagacgg
     1261 gttgctacgc aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc
     1321 gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg
     1381 agcctgctct tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccagc
     1441 acccagcatg ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat
     1501 tgccataaca ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat
     1561 gattccttta ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga
     1621 cctgtccatc agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca
     1681 gcacggatcc catcccctga gaagagctgt agattaccat agtgccgtta ggtgccagac
     1741 ttggcgtttc atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt
     1801 gtttggcaat gtgctgctgc ccaccattgg agctgaccat taccataaat ttaccgtcgc
     1861 tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc
     1921 cgttgatatt cactttataa acctgcggac gaccggcctg gtcagaagta aatgccaggt
     1981 tctggctatc cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc
     2041 ggatctgacc agaagccaaa tccattacgt acaggttcag actaccggtt ttcgacaagg
     2101 cgaatgccag tttgctgccg tctggcgaga atgcaggtgc accgttgtga cgcgggaatg
     2161 aagccacctg acgtacagcg ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc
     2221 tttcgaaggt cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg
     2281 gctgcggtga acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt
     2341 catacgggaa ctgaccgccg ttggtctgaa caacgtaggc aatacgggta cggaacgcac
     2401 ctttaatgcc ggtcagtttt tcaaacactt catcactggc ggtatgacca gcataacgca
     2461 gccactgctt gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag
     2521 tgtcaacaag ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga
     2581 caactacagc gtcaatgccc agtgcggacc atgcagctgg ttgtacttcc tgcgcactac
     2641 ccggctgctg tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt
     2701 cagcagcaac gatgccgcca atatcttcag gtgccgcacc aggccccgcc cactggaaag
     2761 gaacaacacc aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag
     2821 catgcagaac tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca
     2881 tcatatctcc cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta
     2941 aaatacacaa aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtccgaaca
     3001 gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg
     3061 cctggcttgg tggtttcggg atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa
     3121 gtgcgggatc gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta
     3181 tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag
     3241 cagatttaat ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag
     3301 tattaccact cccggcaggc gaagcattgt tccctttcgc ccctcccccc gttttcggtg
     3361 cattcttacc agagcttagc tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg
     3421 cagcggcttt ttcagctgct gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt
     3481 ctgccgctgc tttcttatca gctgctgcct tatcagcagc cgctttttct gctgcttttt
     3541 tgtcggctgc agctttctct gctgccgctt tcttatcagc tgcagcctta tcagctgcag
     3601 ccttttcagc agccgctttc ttctcagctt ctgccttggc tttttcagca gcttcagttg
     3661 ccgctttctt tcttgcttca gctgcagctg cttctgccgc ttccgctttc ttcttcagtg
     3721 ccgcagcggc tacctcggct tttttctgcg cttcggctgc ggctttggcg gcttctgctt
     3781 ctgctttttt cttcgcgtct gcagccgctt tcttcgcagc ttcttccgca gctttagcat
     3841 ctgccgccgc tttcgctgcc gccacttcag cttgcttctg ctttaactcg gcctgttttg
     3901 cggcttcttc agcctgcttt ttctgctcct gagccgctaa ccgctctttc tcaagttgct
     3961 tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc tgctgttcct
     4021 tcatcttgcg ctgttcatca gaacgcttcg cgcttgattc ctggctttgc atgcgtttgt
     4081 actgctcaac taccgcacct gaatcaacca tgacagcgtc gatggacgaa ccaccgccgc
     4141 ctccggctga agcttctata ttctcatcga acgaactcca gatcagcgcc gcaaataaga
     4201 tgacatgcag cactgctgaa ataattatcg cccgcttgag cttgtcgttt tgttcggttg
     4261 cctttgacac tctcggtttc caaaaactgt tcgcctgtta cccgctctct ttcaagcaag
     4321 ggaaacgcag atgtttagat aggctgcgtc attaaaccaa ccgatttcac acccgcacta
     4381 tgtaacaagt tcagtgcttt aattatttca tcgtaaggca catcttttgc gccaccgatc
     4441 agaaagaccg ttttcgggtt ggccttgaaa cggctggaca cttccgccac cacctgctct
     4501 ggtggtaaac gctccaggcg atctttctca accaccacgg tgtactgacc aataccagac
     4561 acttcaacaa tcactggcgg attatcgtta ctgctcaccg cctgtgattc agtagcgtct
     4621 ggcagatcga cctccacgct ctgggtgatg atgggcgctg tcgccataaa gatcagcaac
     4681 agcaccagca gtacgtccag caacggtaca atgttgattt cggacttgag atcgcgacga
     4741 cctcgtccac gcgctctggc catggcttac cccttgttgc tctcgctaac ggtaaacgcc
     4801 tggcggtgca gaatcgcggt aaactcttcc ataaagttgt cgtaattcag ttccagtttg
     4861 tttacgcgct ggttgagacg gttataagcc attaccgccg gaattgcggc gaacagacca
     4921 attgccgtcg caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag tgttgcttgt
     4981 tttaccgccc cgagggcgat aaaggcgtgc atgatccccc agaccgtacc aaacagacca
     5041 atatacgggc tgatggagcc aaccgtaccg aggaacggaa tgtgcgtttc cagattttca
     5101 agttcacggt tcatggagat acgcatagca cgcgacgccc cttccactac ggcttccggc
     5161 gcatggctat tggcacgatg caggcgcaca aactctttga acccgctgta aaagatttgt
     5221 tccgaaccag tcagattatc ccgtttcccc tgactctctt gatagaggcg agagagttcg
     5281 attccagacc agaatttatc ttcaaacgct tcggcttcac gcgccgctgc gttaagaata
     5341 cgggttcgct ggataataat ggcccaggat gcgattgaaa aaccaatcaa aatcaacatg
     5401 ataagtttaa ccagaaggct agccttcagg aacaaatcaa ggatattcat gtcagtcact
     5461 gcttaaactc cgcgacaata gacttgggaa gcgcacgagg cttcattttg agtgggtcaa
     5521 cgcaaacaac cagaacctct gcttcattca gcaaagtatt ctcggcgttg acaatatgtt
     5581 gcgtgaaaac caaagaggtg ccacgcattg atgttatttc agtctgtatt tcgagcatat
     5641 cgtcgagccg cgcaggtgca taatattcca ccgtcatttt acgtaccaca aaggcaacgc
     5701 gttcagccat cagcgcctgc tgactgaagt gatgatgacg cagcatctct gtgcgtgctc
     5761 tttcataaaa agcgacgtaa ctggcgtggt acaccacacc accggcatcg gtatcttcat
     5821 agtagacgcg aaccggccat cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa
     5881 caaaagttag agcttttaaa cgctgctact atacgcgcgc gattgaggtt tgggaatgag
     5941 ggcaagttaa gggagcgtaa aaatttatgg gcctagagcg gcccataatt ttttaaagag
     6001 aattctgatt taaaagaaga agaaaatcag cccgacaatg agaacaatat cggcaagcaa
     6061 tgggcaaaaa atcccttgcc aaagaacctt ctgcggacga aagcccacgc cgtgaatcac
     6121 accagcacag acggcccaca tcagcaataa accatgccag atttccagtt cactggtctt
     6181 cgcggcaaaa cgagatgggt cccaaaacat acatcctgct aacagaagcg ccatcacgaa
     6241 ggaaagcgcc cgcagggggc gcttgtccat taccgcatac aaggtcgcga taatcttact
     6301 catcagatgt cttcttgacc ggctttgcct gattcgacgt gttcaagcgc cagcgcggtg
     6361 attaccccaa acgaacaggc aagaagcgtt cccagaatcc atgcgaaata ccacattttt
     6421 agctccttac ttagtacaga gagtgggtgt tacgttcaat atcttctttg gtgatacgac
     6481 cgaacatttt ccagtaacac caggcggtgt agagcagaat gatcggtacc agaaccaccg
     6541 caacccaggt catgacgtta agcgtcagct ggctggaagt tgcatcccac attgtcagac
     6601 ttgcgttcat catggtgctg gacggcatca caaacgggaa cattgcgata ccggctgtca
     6661 ggatgatgca ggccagcgtc agggaggaga acacaaacgc ccacgcggct ttatccatac
     6721 gtgcagtcag gatggtcagc agcggcagaa ccacacccag tgccggaata gcccacagaa
     6781 ttggcgtgtt gttgaagtta accagccatg cgccagcttc acgaaccact tctttattca
     6841 gtgggttaga ggctgcgtaa tggtccattg tcgatttcac gacataacca tcgataccgt
     6901 acatcaccca tacgccagcc agtgcgaaac agaccagtgt caccagcgca gccacctgag
     6961 ccgttgcacg ggtacgcagg tgcagttcgc ccccggtacg catttgcaga taggttgcgc
     7021 cctgagtaat gatcatccct acgctcacca cgcctgccag caggccgaac gggttaagca
     7081 actggaagaa gttaccggtg tagtacagac gcagatattc atcaacgttg aacggtacgc
     7141 cctgcaacag gttaccgaac gctacaccaa ttaccagcgg cggaacgaag ctaccaatga
     7201 agatgcccca gtcccacatg ttacgccagc gggtttcttc aatcttggag cggtagtcaa
     7261 aaccgaccgg acggaagaac aaagacgcca gcacgaggat catcgccaca tagaagccgg
     7321 agaacgcagc ggcatagacc atcggccagg cagcaaagag tgcgccgccc gcggtgatca
     7381 gccaaacctg gttaccgtcc cagtgtggtg caatggagtt aatcataatt cgacgctcgg
     7441 tgtcgttacg accgaggaaa cgggtgagca tgcccacccc catgtcgaaa ccgtcagtga
     7501 ctgcaaaacc aatcagcaga acgccaacca gcagccacca gataaaacgc aatacttcat
     7561 aatcgatcat ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg gaagactgct
     7621 caaagtgata gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaac ttgaacatta
     7681 agaacaattc tgccaccagg aacagggtat acaggccgca aatcagcacc attgagaaga
     7741 tgagatcgcc tgcggtcagt gacgaattcg ccacagctgt cggcagcact tcaccgatag
     7801 cccacggttg gcggccatat tcagccacga accagcccgc ttctacagca atccacggca
     7861 gcggaatacc gtacagcgcg gcgcgcagaa gccatttttt ctcgccaatg cggttgcgga
     7921 tgacactcca gaaagagagc gcgatgattg ccagcagcag gaagccacac gccaccatga
     7981 tacggaacgc aaagtacagc ggcgctacac gcgggatgga gtctttggtt gcctgttgaa
     8041 tctgcgcttc agtcgcatca gccacgtttg gcgtatagcg tttcagcagc agaccgtaac
     8101 cgaggtcttt cttcatgcta ttgaactggt cacgaaccgc ctggtcggta gaaccagaac
     8161 gcagttgttc gagcagagag tacgccttca tcccgttacg aatgcgttct tcatgctgca
     8221 ccatcagctc tttcaggccg ataaccgggg tatccacgga acgcgttgca atgatgccca
     8281 gtgcgtaagg gatctgaatc gcaaatttgt tcgtctcctc ttcctgatca ggaatgccga
     8341 acaaagtaaa ggcagcaggc gcaggttgcg tttcccactc ggcttcaata gcagccagtt
     8401 tggttttctg cacgtcgccc atttcgtagc cggattcatc acccagaaca ataacagaca
     8461 gaacagcagc cataccgaag ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt
     8521 cacgaccttt cagcatatac catgcgctga taccgaggat gaacatcgcg ccagtcacat
     8581 aaccagacgc tacagtgtga acgaatttca cctgagcaac cgggttaagc accagctcgg
     8641 agaagctcac catctccata cgcatagttt caaagttgaa atcggacgcg attgggtttt
     8701 gcatccagcc gttcgcaatc agaatccaca gtgcggacag gtttgaaccg agcgccacca
     8761 gccaggtgac acacatatgc tgaactttac ccagacgatc ccaaccgaag aagaacagac
     8821 ctacaaaggt ggattcgagg aagaaggcca tcagaccttc gattgccagc ggcgcaccga
     8881 agatatcccc tacatagtgg gaatagtaag accagttagt cccgaactgg aactccatgg
     8941 tcagaccggt agccacaccc agagcgaagt tgataccaaa caacttgccc cagaacttgg
     9001 tcatatcttt ataaatctgt ttgccggaga ggacgtagac cgtttccata atggccagga
     9061 ggaacgccat accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa
     9121 actgtaagcg cgacagttcg actatatcta acatcatgac tccttgctca tcgcatgaag
     9181 actccgagag tgaaccccgt tagaaagggt cacacgcatg ccccaataga aatttgtttg
     9241 aacactttca tcataccatg tgaatgcaca caaaaacggc gtaaagaaag gttgcaaaca
     9301 tgttaataaa aactcaaatt gatcccacgt atatattacg ccgcaaaatc cttacaataa
     9361 acaggttttt gttgaagcaa atttgcattt ttcgacggtg atcaatttat agctaaattg
     9421 ccgcctttca gccaatttga tcgagaacaa tttatctctt tttgatgccc atttccaaga
     9481 cttatacatt gataaatatc aatttctaca aattatgtta atcatgtttc aacaaggtaa
     9541 ctttagtgac atttatgttt aaaatgtgtg aattataaga ctgaaattag tttcggttgg
     9601 caattaaaga taaaaagaga cgaagcaaaa acagcacggt tgaccttaat tctttttttg
     9661 gtcactggct aatacgacgc agagtttcag gattattaat aaaggcataa atctcctccc
     9721 cgccgatatt aacatttaat taacttataa ttatattata attaactcac ccggggttat
     9781 tattttgtta aaaataaagc cacatatacg tagcttgaga gtgaagataa acgcaaaatt
     9841 acctgatgcg ctacgcttgc cagtgtctac atcatctctg caatatattg aatttgcctg
     9901 cttttgtaga ccgaataaag cgttcatgcc gtatccggca tgaacaaagc gccctttgtc
     9961 aacaatctgg ccgcgcatat gtggcttcgg tttgattgct attcaggcaa gccggtaact
    10021 gaacgtccgt gactggccgg gtaaaaaagg ccccgataga tttgaacctt gattttcctc
    10081 ggtggtaatg tgttcatcca tcatcgtttc tgagcaagaa atcacctcgc gactgaaagc
    10141 aacagtcgca tcacaggttg ctgattcagc cggattaaac agccgtaaaa tcacttcttg
    10201 tcggtcttca gctttcttaa gtgcgcttat caggcatccc actgggggca ttttcaacaa
    10261 actataactt tccggcacgt tgaatccggc tttgttgagc ttcatcgcat cccatgggat
    10321 tttgttgtaa cactgtactg gagtcagcca tgctcgcgcc tgctgagcta caccagcggc
    10381 ggttggcgta ccggtataac tcaataaact taggcgacaa gaaagcagac cacgtagttg
    10441 tgagtccggg actggcattt taattcccga aggccgccta ggccttaaaa gcagatcttc
    10501 tttccccagt aagcccacgc cgcgcagcaa cgtaatggca aaggttttct tctcttcacc
    10561 gatgacttca aattcacgta gcccttcgct aaagacagcc atgccgttac gcccttcctg
    10621 taaggctgca tagttgagca tattccatac cggaaccggc gcttctttcc agccttcttg
    10681 ctgccagttg ttcattgcac tgtcgttcac ggggcgcgtt agcgaaccaa actgggtatc
    10741 tgccagaaca ctatcggtgt taaaagatgt agggatcagg acgcgaaggc gatgatcgtc
    10801 agcctggtta tcaaggttga tatccacatc aatgcgcctg ttgttatgac taagagtgac
    10861 aaccaactcg acacctactc tgccagtgga ttgtcttgcg ctgcgttgtt gcagattacg
    10921 cggcaccgcc atttcatagc ggataacagc cctgctctgc caggcctcat gaataatctc
    10981 gcattgcggt ttcgcattcg ccgaagtaat tacccactct tcttttgcgg gtgaatagtc
    11041 atattcatca ccatcatcag agctttcttc aatttgcaac acccggtcat agcgcacacc
    11101 gctatcttta tctaccagtt gtagcgttcc atcctcattg agcgcaattt gccagaaagc
    11161 attttccagt atcccttcag cgtcactttt tgccgcaatc acgttgccag gctgattcgc
    11221 ttcgatataa agcgtgcgat agcccataga agggacaatc tggttgatct gtatatcaaa
    11281 ctccataaag ggatcgtaat taccgtaatg aactatttgc cgatcgatta ggcctggatc
    11341 gatctcacgg gcatggcgaa taaaatacgg tacaggctga ccgcgatcgt ctcgtagatt
    11401 aaactggctg gcgcgcagcc gcacagtggt gttgatcact tcttcccgcg gccagggcat
    11461 caggttaaac aggacgagtt tgtcggcgtc gctctgcggc atgttgtcgg cgattttacg
    11521 catatagaaa cgcagcagat tatccgccat gtcttcagcc agttcgaagc gggcgacgat
    11581 ttcgcgatga actttgtcac tacagcagca gccgatgctg tcgtgggcat gattttttaa
    11641 gatctctttc catattttct ccagcaagcc gtggtggtat tcaaaaccca acgtccaggc
    11701 cagcgttgcc agcggttcca gcagattaac aatcttattt tcaatacgtg cgtgggcaac
    11761 tttgatatcc atgcgcgtgg aaccgatggt gcgatgcacg cgcatatatt tgccatcaat
    11821 aaattcccct ttcagggttg ccagattatc tcgctgcgct tcgatcttct caaatacctc
    11881 ttcaaagcgg ctcatcacaa actttcgttg aggataaatt tcgcgcagtt tctccatcac
    11941 ttcgaagata ttttgctgca atggcatctg atcatgccca ttcggcagca aaatctcttt
    12001 ggttacagac gctttttcca gcacgtcaaa ataactgtcg aggcgtttgc ggaatccgtt
    12061 ttcgtccgca ggtaagtact taccgatggc ataacccaac ggcatcacct gcgccgtcac
    12121 ttcgctaccg tcactgcttt gccacaaaaa ctcggtttta tccgtaccgt ggcgctcaga
    12181 acatccacgc cagaacatgg tgcaggtaat gccaaatcca ttgtagatat gcggcagttg
    12241 cccggacatg ccaaaggaat cgggtaaata acctattttc atcggctcgc caaacgcgag
    12301 gcagtcacgc attccgtaca tcagattacg gacgatggat tccgcagaaa caatcgtggt
    12361 atcggtctgg gtataccagg ggccgataat caacttgccg gcttctacca gtttcttcac
    12421 gcggtctttg ttttccggtt tcaccgcgaa ataatcttcg aggatcgccg tttgcccgtc
    12481 gagtacgtaa tatttgtatt cgttgtcctg ttccagtcgg cacaggatct cttccatatt
    12541 attgaccagc agaatgcgtg actcttcggt ggtgaaatac cactcccgat cccagtgcat
    12601 atgcggggtg atgtgaacgc gagataccgc tttcatcgtc gtttcctttt tgtttattgc
    12661 attacgccat cagtcagata gttgccatgc ttaaccgcgt gacgccgcca gatcaggaga
    12721 attgcagtcg agattgcagc ccccaccaat gccgcaccaa accagccaat tgccgccata
    12781 acaccgcccg cgccattatc atgaagtaaa aagagcgaga aaatgccagc accgggtgtc
    12841 gaaaggccga tattcatcgc accgacaata gcgcccgtta ccatagagcc cagcacaaac
    12901 gaaccaataa cccgcagcgg atcttcaatc gccatcggga tcgccccttc ggtaatacct
    12961 gccagcccta acagccaggt ggatttcccg gtctcaattt caaactcttt aaacaggcgc
    13021 ggtgcgagca tcgtggaagc ggttacggta aatgccgaaa ccattttgac ggaggcgaaa
    13081 atggcatacg ggccgtaaac gccgttcgcc attgcgccca ggcagaatgc ataagcggct
    13141 ttattcactg gccctccaag gtcaaaggaa cacataaaac cgagaatggc acccagcaac
    13201 agcgcgttac ttcctgacag accgttcagc caggcggtaa gcgagttatt gatccaggcg
    13261 acaggttccc ccaccacaaa cagcatcaga ctgcccgctc ccaatgtacc gagcaccagg
    13321 tagagataaa aagtcaggaa gccattgaat ttactgctaa gacgcaagtg atttttcatc
    13381 cagcgtatca agtaaccggc tatcaatccg ccaacgaccg cgccgagaaa cccggagccg
    13441 atcatgttgg cggcaagtcc agccgcaaag cctggcgcta acgccggttt atctgccagc
    13501 gaataggcgg tataggctgc cagcaccggt accatcaaaa ttccgagcag cccgccgccc
    13561 agtttgcggt acatccacag ccaggagttt tcttcattaa acagatcttg tagcccgaag
    13621 atttgcgaca gtaataccgc gaccgccagc accgtccccc ccgcgacaat caacggtacg
    13681 gaaaaagaga tcccgctcaa cagtgcctgt ttcagctccg ttttgacgct tttcaccggt
    13741 tgcgtatctt gctgtacggt acgcgtctca tcgccacgct cgagggtaag cgattgttgg
    13801 atcaacgctt ctgcatggcg aatcggctcg gcaacaggca cagaaagcgc aggaatgccg
    13861 ttaaaacgct cactctcctt gatggcgact tcagccgcaa aaatacaggc agttgcacta
    13921 ttgagttgat ccgccgttaa acgcccttca ataccgttag caccttgctt ttcaacgtag
    13981 acattcacgc cgagtttgcg tccggctttt tccagatact ccgcagccat gtaggtgtga
    14041 gcaatacccg ccgggcaggc cgttacgcaa acgatagttg gcgcgttgga aaaagaggca
    14101 gaaggttgcg tgcctccctt gtcatccagc gccgagagca attcatcagg cgtcgtcgcc
    14161 gactgtatac gcgcccgaat ctcatcatcc gccaggcgcg tggtcagcgc tgtcagcagt
    14221 tgcatgtgcg tagtacccgc ttcattgggg ggaatcgcca gcaacaccac taaatcaatc
    14281 gcttccgggc catccacgct ttcccactga agcggctcgc tgagtgtggc gaccgcaaac
    14341 gccgcttctt ttaccgcagc agttttgcca tgcggcacag ccaacccttc acctaaggcg
    14401 gtcgggccaa ggctttcacg gcgatacact tctttcagaa attgctcagt actggaaatt
    14461 ttccccagag cagcaagacg ttgagtcaac gcgtgaatgg cctcttcacg gctggtaaag
    14521 cgcgcattca gacacaacgc atcgcggtgg gtcagagtcg tcaggttcat catgatcctc
    14581 acttgatcgt ttttatagtc gcgccagtgt gcccgataaa acaataccat tgcgtaatca
    14641 atttcacttg attgaaatta tttgcattgt atttgtatta tttacaggat gaatttatcg
    14701 ggcataatgg ctaaattcgt attaatgaga tgctaaataa tgggacacaa acccttatac
    14761 cgacagattg ctgatcgcat tcgtgagcaa attgcgcgtg gcgagcttaa acctggcgat
    14821 gcgttaccta ccgaatcggc attacaaaca gagtttggcg tcagccgggt tacggtgcgt
    14881 caggctttgc gccagttagt cgagcaacag atcctcgaaa gcattcaggg cagcgggact
    14941 tacgtcaaag aagagcgggt caattacgat atttttcagt taaccagttt tgatgaaaaa
    15001 ctgtcggacc gtcacgtcga tacacacagt gaagttctga tatttgaagt gattccggct
    15061 gacgattttc ttcagcaaca gctacaaatc accgtgcagg atcgtgtgtg gcatgtgaag
    15121 cgcgtgcgct atcgcaagca aaagccaatg gcgctggaag aaacctggat gccgcttgcg
    15181 ttgttcccgg atctcacctg gcaggtcatg gaaaattcga aatatcactt tattgaagaa
    15241 gtgaagaaga tggttatcga tcgtagcgaa caggaaatca ttcctctgat gccaaccgaa
    15301 gagatgagcc gtctgcttaa tatcagccag acaaagccga ttcttgaaaa agtatcgcgt
    15361 ggatatttag ttgatggtcg ggtgtttgaa tatagccgca acgcttttaa taccgatgac
    15421 tataaattta ccctgatagc gcagcgaaaa tcatcgcgat aagcacaaaa aaggccatca
    15481 tacgatggcc aaccatgtcg aaaccgacag cccttcgcca ttcctgtaga gggaagcggc
    15541 gagggctatt tcttattaca gatatttatt tcagaacagt tttcagtgct tcaccgatat
    15601 ccgccaggct gcgaacggtt ttcacgcctg cggcttccag agcagcgaat ttctcatccg
    15661 cagtcccttt cccaccggca atgatcgcac ccgcgtggcc catacgtttg cctttcggcg
    15721 cagtcacacc agcgatgtaa cccacaactg gcttggtaac atgctctttg atgtacgctg
    15781 ccgcttcttc ttcagcgcta ccgccgatct caccgatcat cacgatcgct tcggtctgcg
    15841 gatctttttc gaacatttcg agaatgtcga taaagttaga gccagggatc gggtcaccgc
    15901 cgataccgac acaggtcgac tgaccgaaac cgtaatccgt ggtctgttta accgcttcat
    15961 aggtcagtgt accggaacgg gaaacgatac ccactttacc cggtttgtga atgtgacccg
    16021 gctggatacc gattttgcat tcacccgggg tgataacgcc tgggcagttc gggccgatca
    16081 tacgaacgcc tgcttcgtcc agtttcactt tcacggtcag catatccagc gtcgggatgc
    16141 cttcagtgat ggtgataatc agtttgatgc ctgcatcgat agcttccaga atggagtctt
    16201 tgcagaacgg tgctggtacg taaataacag acgcggaagc gccagtagca gcaacagctt
    16261 cacgcacggt gttgaacacc ggcaggccga ggtgggtagt gccgccttta cctggagtta
    16321 cgccaccaac cattttagtg ccgtatgcaa tggcctgttc tgagtggaaa gtcccctggc
    16381 taccggtaaa gccctggcag ataaccttgg tgtttttatc aattaaaatg gacattattt
    16441 cccctccact gcggcaacaa cctgctgagc tgcatccgtc agaccttttg ctgcaataat
    16501 attcaggccg ctgtcagcca gtttcttcgc gccgagttcg gcgttgttac cttccagacg
    16561 taccacgacc ggtacgttaa cacccacttc cgctaccgcg ccgatgatac cgtcagcgat
    16621 caggtcgcaa cgaacgatac cgccgaagat gttaaccaga acggctttca ctttgtcgtc
    16681 agagaggatg attttgaacg cttcggttac acgttctttg gttgcgccgc cgccaacgtc
    16741 caggaagtta gccggttcgc cgccgtgcag tttaactatg tccatcgtac ccatcgccag
    16801 acctgcgccg ttaaccatac aaccgatgtt accgtccagc gcaacgtagt tcagttccca
    16861 ctgtgcagcc tgtgcttcac gcggatcttc ctgcgactgg tcacgcattt cgcgcagatc
    16921 aggctggcgg aacagggcgt tgccatcagc gcccagtttg ccgtcgaggc aaatcagatc
    16981 gccctgtttg gtgatgacca gcgggttgat ttcgatcagc gccaggtcgc gctccaggaa
    17041 aatggtcgcc aggcccatga agattttggt gaactgctga accagtttac cttccagacc
    17101 cagtttgaac gccagctcgc gtccctgata cggcatcggg ccagtcagcg gatcaagcgc
    17161 aactttatgg atcaggtgcg gagtttcttc cgccactttt tcgatttcca cgccgccttc
    17221 ggtggaggcc ataaagacca cgcgacggga actacggtct acaacggcac cgagatacag
    17281 ctctttagcg atatcggtcg ccgcttcaac cagaatctgg ttaaccggtt ggccattggc
    17341 atctgtttga tacgttacca gacgcttgcc cagccagttt tctgcaaaag cacggatgtc
    17401 ttctttgctg tttacaactt tcacaccgcc cgctttaccg cggccaccag cgtgaacctg
    17461 acatttcact acccacggac cggcaccgat ttttgaagcg gcttcttctg cttcgcgcgg
    17521 agtagtacag gcataaccca ccggtgctgg taagccatag cgggcaaaca gttgttttgc
    17581 ctgatattca tgtaagttca tgtgttctgt ccatccttca gtaatcgtta tcttttaaac
    17641 cgtagaccga caccctatcc acgtcggtct acagtgcagg tgaagcttaa actactacac
    17701 gtccagcagc agacgcgtcg gctcttccag caactctttg atcgttacca ggaagcccac
    17761 ggattcgcga ccatcgatca gacggtgatc gtaggacagc gccaggtaca tcatcggcag
    17821 gatctcaacc tgaccattca ccgccatcgg acgatctttg atagcgtgca tacccagaat
    17881 tgcgctctgc ggcgggttga tgatcggcgt agacatcagg gaaccgaaca caccaccgtt
    17941 ggtgatggtg aagttaccac cggtcagatc ttcaaccgtc agcttgccat cacggccttt
    18001 gactgccagc tctttgattt tcttctcgat gtctgccatg ccgagggtat cgacatcacg
    18061 cagaaccggc gtcaccaggc cgcgcggcgt agaaaccgcc atgctgacgt cgaaatagtt
    18121 gtggtaaacc acgtcatcgc cgtcgataga cgcgttcact tccgggtaac gtttcagggc
    18181 ttcaaccacc gctttcacgt agaaggacat aaagcccaga cggatgccgt ggcgtttttc
    18241 aaacgcttca ccgtactgct tacgcagatc cataatcggc ttcatgttga cttcgttgaa
    18301 cgtggtcagc atggcggtgg agtttttcgc ttccagcaga cgctctgcca cacgcttacg
    18361 caggcgagtc atcgggacac gtttttcact acgtgcagcc agagccggtt gcgccgccgg
    18421 agcagccgct gccggagcag actctttcgc cggggctttc gccagatgtt tttccacatc
    18481 ttcacgagtc agacgaccac ccacaccggt gcctttaatg gcgctggcgt cgagattgtg
    18541 ttcagccagc aggcgacgga tcgccgggct taacgcatcg ttgttttgct cttccagaga
    18601 cgcctgctgg cgttgcgccg gagtggacgc tttctcttca gatttggcgc tggtttcttt
    18661 accggcgctg ttgccttcac gcaggcgacc aaggatctga cgagacgtta ccgttgtacc
    18721 ttcatcttcc agaaccgcat ccagaatgcc gtctgctgat gccggtactt ccagtaccac
    18781 tttgtcagtt tcgatttcta ccagcacttc atcacgtacg actgcgtcgc cgggtttttt
    18841 atgccaggtt gcgacggtgg catcggctac ggattcaggc aggtcaggga ccagaatatc
    18901 tacgctactc attgtgtatc ctttatttat tcgacgttca gcgcgtcatt aaccagatct
    18961 tgttgctgtt tctggtgaac ggacatatac cctaccgccg gagaggcgga ggccgggcgg
    19021 cctgcataac gcagagaagc cccaaacgga atcacttcac ggaaatgatg ctggctgcag
    19081 taccatgcgc cctggttgag cggctcttcc tggcaccaga caaaatcctt gacgtgagca
    19141 aactgctgca acacttcctg catcgcttta tgcgggaacg ggtagagttg ctcgatacgc
    19201 acaatggcga catcgtgttg attgttctta cgacgctgtt ccagcaggtc gtaataaacc
    19261 ttaccagaac acatcactac gcgcttcacg cccttcggat caagttcgtc gatttcaccg
    19321 atggctggca ggaaggtgcc gttcgccagt tcttcgaggc tggaaaccgc cagcggatga
    19381 cgcagcaggg atttcggcga catcacgacc agcggacgac gcatcccgcg cagcgcctga
    19441 cgacgcagca tgtggtaaac ctgtgccggg gtagacggta cgcaaacctg catgttttgc
    19501 tcagcacaaa gttgcagata acgttccaga cgcgcggagg agtgctccgg cccctgccct
    19561 tcgtaaccgt gcggcagcaa catcaccaga ccacacatcc ggccccattt ctgctcgcca
    19621 gaggagatga actggtcgat aaccacctgc gcaccgttgg cgaagtcacc gaactgcgct
    19681 tcccagatgg tcagggtgcg tggttctgcg gtggcataac catattcaaa cgccagcacg
    19741 gcttcttctg acagtacgga gtcccagaca cggaacgcgc cctgaccgtt atggatgtgt
    19801 tgcagcggcg tgtaagtgga accgttagac tggttgtgga tcaccgcgtg gcggtggaag
    19861 aaggtaccgc gaccggagtc ttcacccgac aggcgaaccg gaatgccttc atcaaccagc
    19921 gtggcgtaag cgaggttttc cgcaccgccc cagtcgaaca gtttctcacc ggctgccatc
    19981 gcctggcgat cgccataaat cttggcaacg cgagactgca tttcaactgc ttctggtacc
    20041 gtactgatac gtttagccag ttcctgcagg cgcttcatct caactttgtt cgggtactct
    20101 tcgtcccatt cgtggttgag gtacggcgac caggtgaaag agtgcatgtt catcggacgc
    20161 cactctgcca ctacgcaatc gccagcatcc agcgcatcgc ggtacaggtt aaccatctcg
    20221 gtggcatctt ccagcgtcgc cactttttcc tgctccagct tgtcagcgta gattttgcgc
    20281 ggtgtcggat gttttttgat tttctgatac atcagtggct gggttgcgct cggctcgtcg
    20341 gcttcgttgt ggccgtgacg gcggtagcat accaggtcga tgaagacatc acgtttaaag
    20401 gtgttacgga aatcgagcgc cagacgggtc acaaaggcaa cggcttccgg atcatccgcg
    20461 ttaacgtgga aaatcggtgc ctgaaccatc ttaccgatat cagtacagta cggtgtagaa
    20521 cgggcatcca gcgggttaga ggtggtgaaa ccaacctggt tgttgataac gatacgtacc
    20581 gtaccgccaa cttcataacc acgcgctttc gacatgttca gagtttcctg aaccacgccc
    20641 tgcccggtca ctgcggcgtc accgtggatg gtgattggca gcactttgtt gctgctcggc
    20701 tcatcaagtc tgtccagacg ggcacgaaca gagccgataa cgaccgggct tacaatctca
    20761 aggtgagacg ggttaaacgc cagcgccagg tgcaccaggc cgccatcggt ctggaagtca
    20821 gacgagaagc ccatgtggta tttcacgtcg cctgtgccga ggtgttcttt atgtttaccg
    20881 gcgaactcgt cgaacaagtc ttgcggtttt ttacccagca cgttcaccag cacgttcaga
    20941 cgaccacggt gcgccatccc gagaaccact tcgcgggtgc cgctgttgcc agcgtggcgg
    21001 atcatctctt taagcatcgg gattaacgcg tcaccgcctt ccagcgagaa gcgttttgcg
    21061 ccagggaatt ttgcgccgag gtaacgctca aggccttcag cggcggtcag ttcgcttaag
    21121 aagcgttttt tctcttcgct attgaaagtc gcgcgaccag actcgatacg ctgttggatc
    21181 cagcgttttt cttcggtgct ggtgatgtgc atatactcgg caccaatcgg gccgcagtag
    21241 gtttgcttga gggcggaaat aagctcgccg agtttcatgg tttctttgcc gctggcaaat
    21301 gaaccgacgt tgaaggtttc ctggaagtct gcttcggtca gatcgtggaa agacggatcc
    21361 agatcggcca ctttatcttg ctgccacagt cccagcggat ctagattcgc atgctggtga
    21421 ccacggaagc ggtatgcgtt aatgagctgc aggactttaa cctgcttcac attggtgtca
    21481 gggtcggaga tcgttgaaga gtaacgtgaa gcgtctttcg ccaggcggcg gaaatattca
    21541 cgcgtttgag agtggaattg atccggtttg actcccgtac caggtaactg ctggaacgtc
    21601 gaacgccagt tagcgtcaac cgagtcagga tcggttaaga agtcttcata gagctgttct
    21661 atccagctct ggtttgcgcc agagaggtaa gaagagtcca accaggcttt caaagcgctg
    21721 ttctgcatcg tgatccctta agcatctttt ttatgcttac ttcgccgtgg atactaccac
    21781 gcacagtgat gtgcgtgccg gggttcactt gcgagctctt gtctacgtag tggctcgcga
    21841 aggaaccttt aaaaactgtc ttagtgtcag gggcagtttt taaagatttc ctgcatttct
    21901 ttgttacgcc tgatgcgctg cgcttatcag gcctgcgggt aatgacttgt aggccggata
    21961 aggcgttcac gccgcatccg gcactggttg cctgatgcaa cgcttgcgcg tcttatcagg
    22021 cctacagttt acgcattacg ttgcaacaac atcgacttga tatggccgat ggcgcgcgtc
    22081 gggttcagcc ccttcggaca tacactgacg cagttcatga tgctgtgaca gcggaatacg
    22141 ctgaatgcat cgctcaaacc gtcgaggcgg ctgtcagtct cggtatcacg gctatcgatc
    22201 aggaaacgat atgccgctaa caagcctgcc gggccgataa acttatcggg attccaccag
    22261 aaagacggac aagaggttga acaacatgcg cagagaatac attcatacag cccgtcgagt
    22321 ttttcgcgct gctctggcat ctgtaaatgc tcgcgagctg gcggattttg tccattattc
    22381 aacaggtaag gcttaatttt ctcatattgc gcatagaatt gtcccatgtc taccaccaaa
    22441 tcgcggatca ccggtaaacc tggcagcggg cgaatcacaa tcttcttgcc cggctggttg
    22501 agtgccgaaa tcggggtaat acaggccaga ccattcttgc cgttcatgtt cagaccgtcg
    22561 gaaccgcaca caccttcacg gcaggagcgg cggaacgaca ggctgggatc tttctctttc
    22621 agctggataa gcgcatccag cagcatcatg tcgcgacctt cttccgcttc cagggtgtaa
    22681 tcctgcatac gcggagcatc atcaacatcc gggttatagc gataaattga aaactcgagt
    22741 ctcattttcc tgtctccgca ttagtaagta cgaatcttcg gcgggaatgc cgggcgcagt
    22801 ttcggttcca tgttgacgct tcggcgcgtc atggattccg actcaggcag atacagggag
    22861 tggcacagcc agttttcatc atcacgatcc gggaagtcga agcggctatg tgcgccacgg
    22921 ctttcggtac ggaagttggc agaaacagcc gttgcatacg ccgtttccat caggttatcc
    22981 agttccaggc actcaacgcg ctgggtgttg aactcgctgg aagtgtcatc cagacgggca
    23041 tttttcagac gctcgcggat cactttcaac tgctcaagcc ctttcgccat cgcatcacct
    23101 tcacggaaga ccgagaagtt atgctgcata cattcttgca gcgctttacg gatcgccacc
    23161 ggatcttcac cgttacgatt attgttccac cggttcaggc gatccagaga cgcttcaaca
    23221 tcagactcgc tggcatcgcg cagtgcgccc tgctcggcga tagactcttg cagatgcaga
    23281 cctgccgcgc gaccaaagac caccaggtcc agcagcgagt tgccgcccag acggttagcg
    23341 ccgtgtaccg atacacaagc gatttcacca acggcaaaca gtcccggaac aaccacatct
    23401 tcgcctttct cattcacagt cagcgcctga ccggtaactt tggtcggaat accgcccatc
    23461 atgtagtgac aggttgggat aaccggaatc ggctctttca ccggatcgac gtgagcgaag
    23521 gtacgggaaa gctccaggat acccggcaga cgggattcga gaacttcttt acccaggtga
    23581 tcgagtttca gtttcgcgtg tggcccccac ggaccatcac agccgcgacc ttcacggatt
    23641 tcaatcatga tggaacgcgc caccacatca cggcccgcca ggtctttggc gttcggcgca
    23701 taacgctcca taaagcgttc gccatgtttg ttcagcagat aaccgccttc accacggcaa
    23761 ccttcggtga ccagtacgcc cgcaccggca atgccggtcg ggtggaattg ccacatttcc
    23821 atatcctgca ccggtacgcc tgcgcggata gccatgccga caccgtcgcc agtgttaatg
    23881 tgggcgttag tggtggactg ataaatacgc cctgccccgc cagtcgccag cacggtagcg
    23941 cgggctttga aataaaccac ttcaccggtt tcgatgcaca gtgcggtaca acccaccacc
    24001 gcgccatcct ggtttttcac cagatccagc gcataccact cggagaaaat ggtggtgtgg
    24061 tttttcaggt tctgctgata aagcgtgtgc aacagtgcgt gaccggtacg gtcagcagcc
    24121 gccgcagtgc gtgccgcctg ctcgccgccg aagtttttcg actgaccgcc aaacggacgt
    24181 tgatagatac gaccatcatc gagacgcgag aacggcaggc ccatgtgttc gagttccaga
    24241 atcgcttccg gcccggtttt acacatatat tcaatcgcgt cctggtcacc gatatagtcc
    24301 gaccctttta cggtgtcgta catatgccat tcccagttat cttcatgggt attacccagc
    24361 gcaacggtaa tgccgccttg cgcagaaacg gtatgggaac gggtcgggaa gactttagag
    24421 agcagcgcac aggtctggcc gctctgggaa atttgcagcg ccgcgcgcat acctgcgcca
    24481 ccggcaccaa tcacaactgc atcaaattct ctgactggca atttcatcac accccccaca
    24541 ccacaacgaa tccataaatc acgtaaacca ccagtgcaac gacaatcacc agttgcagca
    24601 tcaggcgcaa ggccagcggt ttaacgtagt cggtcaacac ctgccacatg ccgatccagg
    24661 catgaatcaa gatagaaaac agcgccagca gggtgaacac tttggtgaac gcagaggcga
    24721 agaaaccgat ccagacttca tatgtcagtt cgccactggt ggcgaaaaaa ccgaccatat
    24781 aaatgatgta gagcgtcagg acgatagcgg tagcgcgaac gaggatgaaa tcatgtacgc
    24841 cattgcgtcc taatgcggag gcgttgctta ccatacgagg actcctgcga gaagtgaaag
    24901 cacgacagta ataacaaagg agattttggc ggagcgttta cccgcttcga atgtttcttc
    24961 cagatagcca aaatccatca tcatgtggcg aatacctacg acgacgtgat acgccagagc
    25021 ggtaaggatg ccccacatga taaatttgac gaagaagctg cccataatcg cggaagcttg
    25081 ctcgaaacct tcaggggaag agaggctggt acccagaagc cacagcagga tgcccactgc
    25141 aacaaaggtg atcacaccgg aaacgcgatg gagaatggac gctatcgccg tgatggggaa
    25201 ccggatggtc tgtaggtcca gattaacagg tctttgtttt ttcacatttc ttatcatgaa
    25261 taacgcccac atgctgttct tattattccc tggggactac gggcacagag gttaactttc
    25321 tgttacctgg agacgtcggg atttccttcc tccggtctgc ttgcgggtca gacagcgtcc
    25381 tttccataac tgcgcgtcat gcaaaacact gcttccagat gcgaaaacga cacgttacaa
    25441 cgctgggtgg ctcgggattg cagggtgttc cggagacctg gcggcagtat aggctgttca
    25501 caaaatcatt acaattaacc tacatatagt ttgtcgggtt ttatcctgaa cagtgatcca
    25561 ggtcacgata acaacattta tttaattttt aatcatctaa tttgacaatc attcaacaaa
    25621 gttgttacaa acattaccag gaaaagcata taatgcgtaa aagttatgaa gtcggtattt
    25681 cacctaagat taacttatgt aacagtgtgg aagtattgac caattcattc gggacagtta
    25741 ttagtggtag acaagtttaa taattcggat tgctaagtac ttgattcgcc atttattcgt
    25801 catcaatgga ttctttacct gcaagcgccc agagctctgt acccaggttt tcccctcttt
    25861 cacagagcgg cgagccaaat aacaaacggg taaagccagg ttgatgtgcg aaggcaaatt
    25921 taagttccgg cagtcttacg caataaggcg ctaaggagac cttaaatggc tgatacaaaa
    25981 gcaaaactca ccctcaacgg ggacacagct gttgaactgg atgtgctgaa aggcacgctg
    26041 ggtcaagatg ttattgatat ccgtactctc ggttcaaaag gtgtgttcac ctttgaccca
    26101 ggcttcactt caaccgcatc ctgcgaatct aaaattactt ttattgatgg tgatgaaggt
    26161 attttgctgc accgcggttt cccgatcgat cagctggcga ccgattctaa ctacctagaa
    26221 gtttgttaca tcctgctgaa tggtgaaaaa ccgactcagg aacagtatga cgaatttaaa
    26281 actacggtga cccgtcatac catgatccac gagcagatta cccgtctgtt ccacgctttc
    26341 cgtcgcgact cacatccaat ggcagtcatg tgtggtatta ccggcgcgct ggcggcgttc
    26401 tatcacgact cgctggatgt taacaatcct cgtcatcgtg aaattgccgc gttccgcctg
    26461 ctgtcgaaaa tgccgaccat ggccgcgatg tgttacaagt attccattgg tcagccattt
    26521 gtttatccgc gcaacgatct ctcctatgcc ggtaacttcc tgaatatgat gttctccacg
    26581 ccgtgcgaac cgtatgaagt taatccgatt ctggaacgtg ctatggaccg tattctgatc
    26641 ctgcacgctg accatgaaca gaacgcctct acctccaccg tgcgtaccgc tggctcttcg
    26701 ggtgcgaacc cgtttgcctg tatcgcagca ggtattgctt cactgtgggg acctgcgcac
    26761 ggtggtgcta acgaagcggc gctgaaaatg ctggaagaaa ttagctccgt taaacacatt
    26821 ccggaatttg ttcgtcgtgc gaaagataaa aatgattctt tccgcctgat gggcttcggt
    26881 caccgcgtgt acaaaaatta cgacccgcgc gccaccgtaa tgcgtgaaac ctgccatgaa
    26941 gttctgaaag agctgggcac caaagatgac ctgctggaag tggctatgga gctggaaaac
    27001 atcgcgctga acgacccgta ctttatcgag aagaaactgt acccgaacgt cgatttctac
    27061 tctggtatca tcctgaaagc gatgggtatt ccgtcttcca tgttcaccgt cattttcgca
    27121 atggcacgta ccgttggctg gatcgcccac tggagcgaaa tgcacagtta cggtatgaag
    27181 attgcccgtc cgcgtcagct gtatacagga tatgaaaaac gcgactttaa aagcgatatc
    27241 aagcgttaat ggttgattgc taagttgtaa atattttaac ccgccgttca aatggcgggt
    27301 taatttttat atgcctaaac acaaaaaatt gtaaaaataa aatccactat cggacattca
    27361 tatattctaa aaaaaaatat atcggtataa attataaaca aatcgatgat ttttaatcat
    27421 ctatcttcct ctcagttaca tgaataaacc accaaatata gcgcacccct ataatttcca
    27481 atactttatt catcaaacat ttaattcata ccatcaatta attactttta catttaataa
    27541 aattaataat atttttaaga aaaactaaaa acattcgaca atcaaataag atattcttgc
    27601 accgatatac ttttttcttt tctgttaaag gactcagtta tgtttaaagg acaaaaaaca
    27661 ttggccgcac tggccgtatc tctgctgttc acagcacctg tttatgcggc tgatgaagga
    27721 tccggtgaaa ttcactttaa aggtgaagtt attgaagcac cgtgtgaaat acatcaggat
    27781 gatattgata aagaggttga actcggtcag gtgaccacca gccacattaa tcagtcacat
    27841 cacagcgatg ccgttgctgt cgacctgctc ttagtcaact gtgatctgga aaactccagc
    27901 aacggttccg gtggcaagat ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc
    27961 accggcgcag atccgattct caacaacacc agcacgggtg aagccacctg cgtcggcgta
    28021 cgtttaatga ataaagatca aagcaatatc gttttaggca ccgctactcc agatatcgac
    28081 ctggctccga cctccagcga acaaacgctg aatttctttg cctggatgga acaaattgat
    28141 caggctacac ctgtaacgcc aggcgcagtt acggcgaatg caacctacgt gctggattat
    28201 aaataagtat tactttggcg gcgctatcgc cgcctttcat aaaatattta tcaggtatgg
    28261 acaccgtgaa tatttatcga ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg
    28321 cattggcgct cgaattcaat ctgaatgttc tcgataaatc aatgcgcgac cgcattgata
    28381 tttcattatt aaaggaaaaa ggagtcattg ctcccggtga gtattttgtt agcgttgcgg
    28441 taaataataa ccaaatcagt aacgagcaaa agattaactg gcacaaaaat gacgataaaa
    28501 ccattccgtg catcaatgat ttactggtcg ataaatttgg cttaaaacct gaagtccgtc
    28561 agtcattacc attgataaat cagtgcgtcg attttagctc ccgacctgaa atgctcttca
    28621 atttcgatca agccaatcag caactgaata ttagtattcc gcaagcctgg ctggcgtggt
    28681 actcagaaaa ctggacccca ccctccacat ggaaagaagg tgtcgccggt gtcctgatgg
    28741 attacaactt gtttgccagc agctaccgcc cacaggatgg cagcagcagc actaatctta
    28801 acgcctatgg aacagccgga atcaacacgg gggcatggcg cttacgcagt gattatcagt
    28861 tgaatcatac tgatagcgat gataaccatg aacagtcagg cgaaatatca cgtacctatc
    28921 tttttcgccc gttaccgcaa ttaggttcaa aattaaccct cggcgaaacg gattttagtt
    28981 ccaatatttt cgacggtttt tcttataccg gcgcggcact ggcaagtgac gatcgaatgt
    29041 tgccatggga actacgcggc tacgccccac aaattagcgg tattgcacag accaatgcca
    29101 cggtgacgat cagtcaatca ggccgcgtca tttaccagaa aaaagtccca ccaggcccat
    29161 ttatcattga cgaccttaat cagtctgttc agggcacact ggatgtcaaa gtgacggaag
    29221 aagatggtcg ggtgaacaat ttccaggttt cagcagcatc gacgcccttc ctgactcgtc
    29281 agggacaggt tcgctataaa ctggccgcgg gtcagccacg gccctccatg tcacatcaaa
    29341 ctgaaaatga aacctttttt agcaatgaag tttcctgggg gatgctgtca aacacctcgc
    29401 tgtacagcgg cctgctgctt tctggtgatg actaccattc tgccgcaatg ggtattgggc
    29461 aaaatatgct gtggcttggt gcgctgtcgt ttgatgtcac gtgggccagt agccattttg
    29521 atactcagca ggacgagcgg ggcttaagct accgttttaa ttacagcaaa caagtggatg
    29581 ccactaacag cacgatttcc ctcgccgctt atcgtttctc cgatcgtcat tttcacagct
    29641 acgccaacta tctggatcac aaatacaacg acagcgatgc gcaggacgaa aaacagacga
    29701 tcagcttatc tgtgggccaa ccgattaccc cactaaacct caatctttac gccaacctgc
    29761 tacatcaaac ctggtggaat gcagacgcct ccacgaccgc caacatcacg gcaggtttta
    29821 atgttgatat tggtgactgg agagatatct ctatttctac gtcattcaat acaacccatt
    29881 acgaagataa agatcgcgac aaccagattt acctgtcgat ttcgctcccc ttcggtaacg
    29941 gtggtcaggt tggctatgac atgcaaaaca gtagccacag caccacacac cgcatgtcgt
    30001 ggaacgatac gctggatgaa cgtaatagct ggggcatgtc tgccggactg caatccgacc
    30061 gtcctgacaa tggagcccag gtgagcggta actatcagca cctgagttca gcgggtgagt
    30121 gggatatttc tggtacctat gccgccaatg attacagttc cgtcagcagc agctggagcg
    30181 gttctttcac cgcaacccaa tatggtgcag cgtttcatcg ccgcagctcc accaatgaac
    30241 cacgcctgat ggtcagcacc gatggcgtgg cagatattcc ggttcagggc aatctcgact
    30301 acaccaacca ttttggcatt gcggtggtgc cgttgatttc cagttaccag ccttccaccg
    30361 tggcggtgaa catgaatgac ttacccgacg gcgtaacagt tgcagaaaac gttatcaaag
    30421 aaacgtggat tgaaggcgcg ataggttaca aatcactggc ttcccgttcc ggtaaagacg
    30481 ttaacgtcat cattcgcaac gccagcggtc agtttcctcc cctcggagcg gatatccgcc
    30541 aggatgacag cggcattagc gtggggatgg ttggcgagga aggacatgcc tggttaagcg
    30601 gagtcgctga aaatcaaaag tttaccgtgg tctggggtga tagccagcat tgctcgctcc
    30661 atcttcctga acatatggaa gacaccgcaa atcgcctgat tttaccttgt cattaattaa
    30721 ggaaatgaaa atgacattta tgaaaggact accccttttg ttgttggttg ccagtctgtg
    30781 cagccacgct gcactacaac ccgatcgcac tcgtattgtg tttaacgcta atgataaagc
    30841 taccagtctg cgggtggata atcgtagcga taaactcccc tacctcgctt attcctggct
    30901 cgaaaatgaa aaaggggaga aaagcgatga tcttctggtc gccctgccgc cgatacaacg
    30961 tctggaaccg aaagcaacaa cgcaggtacg gatagtaaag caagcctcga ccacaaaatt
    31021 accgggcgat cgcgaaacgc tatttttcta caatatgcgt gaaattcctc ccgccccgga
    31081 aaaaaacagc gaccatgccg ttctgcaagt cgccattcag agtcgtatca aggtgttctg
    31141 gcgaccggct gcattacgca agaaagcagg agaaaaggtc gaactgcagt tacaggtcag
    31201 ccagcagggc aaccaactga cactgaaaaa tcctaccgcg tattatctga ccattgccta
    31261 cctgggacga aatgaaaaag gcgttctccc cggcttcaaa accgtgatgg ttgcgccttt
    31321 tagtacggtc aacacaaaca ccggaaatta tagcggcagc cagttttatc tcggttatat
    31381 ggatgattac ggcgcgttac gcatgacgac gctcaactgc agcggacaat gccatttaca
    31441 agccgtggag gcgaagaaat gagtgctggc aagggagtgt tgctcgtcat ctgtctgtta
    31501 tttctgccgt tgaagtctgc gcttgccctg aactgctact ttggttcatc cggtggctcg
    31561 gtagaaaaat cagaagcgat tcaacctttt gctgtaccag gcaatgccaa actcggcgat
    31621 aagatctggg aatctgacga tattaaaatt cccgtctatt gtgacaacaa taccaacggc
    31681 aactttgaaa gcgaacatgt ctacgcatgg gtaaacccct acccgggggt gcaggatcgt
    31741 tattatcaac tcggagtaac ttacaatggc gttgattatg acgccaacca gggaaaaagc
    31801 cgcatcgaca ctaaccagtg tatcgacagt aagaacattg acatttatac ccttgagcag
    31861 atcatcgcga tgggatggca gaacaaaatt tgctcgggtg atcccgctaa tattcacatg
    31921 tcgcgtacct ttctcgctcg catgcggtta tacgtcaaaa tacgagaaat gccgccgcat
    31981 gattatcaaa gtacgcttag cgactatatc gtcgtgcaat ttgacggtgc cggtagcgtt
    32041 aacgaagacc ctactgccca aaacctgaaa tatcatatta ctggtctgga aaacattcgc
    32101 gtgctggatt gcagcgtcaa tttttccatt tcaccggaaa cacaggtgat tgattttggt
    32161 aaatttaatt tgctggatat tcgtcgccac acaatgtcga aaacattcag tattaagacg
    32221 acaaaatcac aaaatgacca atgcaccgac ggatttaaag tcagttcctc cttttatacc
    32281 gaagaaacgt tggttgaaga agataaagca ttgttgattg gtaatggtct gaagctacgt
    32341 ttgctggatg aaaacgcctc gccttatacc ttcaataaat atgctgaata cgccgatttc
    32401 accagcgaca tgttggtcta cgaaaaaacc tatacagctg aactttcgtc cattgcaggt
    32461 acccccatcg aggctggggc ccttcgatac agtggtgctt tttaaaatta actataactg
    32521 atttgccatg atgccctttg cagccggaaa gggcatctct ttcagaaaat tgctgatttt
    32581 ccccttttcc ttgattgctg ttattttttc ctagcccggc tttctttatg ctgtatcgat
    32641 acaccaaaga gaaaataatg agggagcgaa ggatgccagt tttgcagtgg ggaatgttat
    32701 gtgtgctatc acttctcctt tctattggtt tcctcgcggt acatctcccg gcggcgctat
    32761 tacttgggcc gatgatcgcc gggatcatct tcagtatgcg cggaattact ctgcaacttc
    32821 cccgctccgc ttttctcgcc gcgcaggcca ttcttggctg catgattgcg caaaacctca
    32881 ctggttcgat tctcaccacg ctggccgcca actggccgat cgtgcttgct attttactgg
    32941 tgacgctgct ttccagcgcc atcgtgggtt ggttattggt gcgctatagc tcactgcctg
    33001 gaaataccgg tgcctggggt tcttctccag gcggcgcagc ggcaatggtc gctatggcgc
    33061 aagattacgg cgcagatatt cgcctggtgg cgtttatgca atatctgcga gtgctgttcg
    33121 tcgcgggtgc cgctgttctg gtcacccgca tgatgctggg tgataacgct gaagcggtta
    33181 accagcaaat cgtctggttc ccgccagtga gcattaatct cctgcttacg attttgctcg
    33241 cagtcgttgc cggtacggcg ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc
    33301 cgatgctggc gggcgcagtg ctccagtctg gtcagctcat caccatcgaa ctaccggaat
    33361 ggctgctggc gatggcgtat atggcaattg gctggcggat tggtcttggt ttcgataaac
    33421 aaatcttact gcgggcacta cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc
    33481 tggctatttg tgcaggtatg gcgtggggac tgacccggtt tatgcatatt gattttatga
    33541 ctgcctacct cgccaccagt cccggcgggc ttgataccgt ggcggtcatc gccgcaggga
    33601 gcaatgccga tatggcgctt attatggcga tgcaaaccct gcgcctgttc agtattttgc
    33661 tgacagggcc agccattgcg cggtttattt caacctatgc gccgaagcgg tcggcctagt
    33721 gctggcagcc tggacaccag taaaacgggc gcgaggacaa cgtggttttc tcaatgatgc
    33781 cgccacagcg ttcgcacagt tcgccatcac gatgaaaaac cttaaagcga aacagcgccc
    33841 catgatgctt attctcatcc acctgccccc gcgtagcgta agaaaaccga ggaatctcca
    33901 gtaacgcgtg tgcgagtgca tccagttgcg ccgcattgag atctttcgct ttatgatttc
    33961 cagtcaaccc aacctgccag aggatctcca cccgcaaata attgccaagc ccagccagaa
    34021 acgcctgatc gagcagtaat cctgcaaact gacggttacg aaagcgcggc gacaataacc
    34081 gttcctttac cacctccggc gtcagattcg gatccagcac atcgggacca acgcgttgta
    34141 aaaacggatg cgtggtcagt tgttccggcc tcaacatctc aatatccgag gcgctataaa
    34201 gcagaatggt tttgtcagcc gtttgcagtt ttacccgcaa tactcgcgtg gtctgcggct
    34261 cttcgccggt atcaaccacg cgccagacgc cgtaaagctg attatggctg tagagcgtta
    34321 aaccgttggg aaaatgagtt aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt
    34381 gaccgataag ttgtgattga taagttttta actgcgggaa ggcaaaccag acatcagtta
    34441 gtggtttgcc tttgatcgcc gcctccaggt tatccgctgc acggcggatc tccgggcctt
    34501 caggcatggt gatatccttt tttcattctt tattaagcgg ggttatgctg cgacaacaat
    34561 cccctttttg gcaaatgtag ctcgtaatcg gcgggcgaac gccagagcgt gctcgccgtc
    34621 gccatgcagg cagaccgttt gcgccgcgac cgttgcccat tcgccggtga tacttttgac
    34681 tctgccgtgt tgcaccatct ccagcgtttg cgccaacgcc tgttcttcgt tttcaatcaa
    34741 cgcgcctggc tgacttcgcg gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa
    34801 cacttcctcg cgcgttgtca ggccatattg cttgcctgca cgaatcagct cgcttcctgc
    34861 cagcccaacg agaaccaatg ccggatcgca agcgtatacc gctctggcga tggcgtctgc
    34921 cagttgtgct tctttcgccg cctggttgta caacatgccg tgcggtttga catgacgcat
    34981 tacgccgcct tgcgcacggg caatcgttgc cagcgcgcca atttgataca gcgtctgggc
    35041 gtaaacggtt tccggcggca gctgcatggc gctgcgacca aaattttccc tgtcgggaaa
    35101 actcgggtga gcgccaatcg cgacaccatt ttttattgct tcacgcacgc aagcctgcat
    35161 ggtttgcgca tcgcctgcat gaaatccaca agcaatattg gcagaggaaa ccagcgttaa
    35221 tagctctgcg tcgctggcgc agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt
    35281 gcagccgcca cgctaattgt tcaaaataac gttgctgatc ttgccgcgct ttcagtgcct
    35341 cttccagtga acactggaca aaatgaatcg gctgaccgag cggaatttgc gccagatggt
    35401 acatatcagc ctcaatgata caggcaatac gtggataacc accggtggtc tgtgcgtcgt
    35461 tcatcaacac aatcggctgc ccgttatgcg gcacctgcac cacgcccggt aacaaaccgt
    35521 gagataacag ttcgcgatcg gtgatgcgtt ttaaaatttg cccctgtaaa cgatagccca
    35581 ttcggttact ttgcgagcta agctgccagg gcgaacgcca gaatgcatcc tgcgaggcgc
    35641 gatcgaactc atgatattcc ggccccggca aggcgcgaat gcggttgccc cacagcagct
    35701 gtttaacgcc ctgcgcttcc ataaaatcac gcttcgattt gccaatcggg agtcggtcac
    35761 catccttcag taaacggcct tccagcccgc caatccccac tttgagatcg gtgctgcatg
    35821 accccattac cggcggaaca tcaataccac ccgcgaccgc cagataactg cgcatcccgt
    35881 gctgcgggcg tttaagcgtt aaacgctggc ctgctttcat cggcaatcgc cagccggtcc
    35941 agacggcgtt atcatccaac cgcgcttcgc aacctgcacc cgtcagggca aaccacccat
    36001 cagtttcgaa ctcaactgtt aactgaccga gcgtgatctc cagcgcgggg gcattggcgt
    36061 cattacccac cagcaggtta gcaatgcgca gcgcaggcat atccagtgcg ccacagtggc
    36121 tgatacccga ctggcgaaaa ccgtgacgac cgccatcctg cacagtggtg tacatgcccg
    36181 cacgaataat cttcagcata ttccctcctt ctgcggtaca aatcgcacgc tgtctcccgg
    36241 acgtaataag atgggttcgt cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat
    36301 caactgccag ccacccggcg ttgccagcgg ataaacacca gtctgcggcc cgccgatccc
    36361 gacagaacct gccggaacga gcaagcgcgg ttcagcgcgc cgtggcgtgt gttgttgttc
    36421 cggcaaactc ccgagatacg ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc
    36481 cacggaggag tgcaattcaa caacctgttt ttcgctcaac ccgcaatacg ccgcgaccac
    36541 cgccaaatcc ggtccgcctg caccaccgta aaccaccgga atttcaataa agcgagactc
    36601 aggttccagg gcctcgctct cctcccacca gcgttgcaaa cgctctatgg catccagcgc
    36661 cagcgactca ggattacgca aaatcaccgt gatattgttc atgccgggaa tggcttcaac
    36721 cacattcggc atatccacca gacgctgcgc cagtcgccag atccgcttct ggctagccag
    36781 cgtcaccggc ggttccagtt ccagcactac cgccgtttca cctatcagat aacaacgcgc
    36841 tcgttgcact ttcgatccct ctgattattc gttatgcagg attaggaata tcaataaagg
    36901 tcacatcaag atcggtattt tcattcagcc actcgctcaa tgcgcgaata ccaccgcgtt
    36961 cagtggcatg gtgacctgca gcataaaaat gcaatccctg ctcgcgggct gaatgaatgg
    37021 tctgttcaga aacttcgcca gtaataaaag catccacgcc aaaacgcgcg gcgctatcga
    37081 taaaactttg cccgccgccc gtgcaccagg cgacgcgctg gaccacctca ggtccggtat
    37141 cgccacacca taacggctta cgtcccagac gcgcttcaat ccaggaggct agttccattc
    37201 ccggcacagg catggtcagc tctccccacg gcaccaacgg ctcaatttcg cccatgaccg
    37261 tgatccccag taacgccgcc agctgtgcgt tattgcccag ctcaggatgt gcgtcaagcg
    37321 gcaaatgcca gccatacagg ttgatatcat tcgccagcaa cgtttttaaa cggttacgct
    37381 tcattccgcg aatgaccgga gactctcctt tccagaagta gccatgatgc acaatgaccg
    37441 catccgcgcc cagacgcact gcctcatcga gcaaagcctg gctggcggtg acaccggtaa
    37501 caattttttg caccgtctct ttgccttcca cctgcaaacc attcggcgcg taatcactaa
    37561 tcgccgcgct gttcagtttt tcgttaatca gttgttccag ttcggtgttt ttcatcttcg
    37621 cgccttccgt attctgatag ctacaccgtt aacatagcgt tacctcgcct gttcgtcgat
    37681 agccaatctt ttgacatatt aagaaacaaa cccgacgcca aaagacgcaa attgtttata
    37741 tcatcacgct tcagctaatg tatatttatg caatcagaca gcatattgca taaataatca
    37801 gttctgtatc aaagttttaa ctttaaggaa acacaagcac gccgcgcaac tattacgtga
    37861 cgttgccgtc aattgtggaa ttattatgaa taaacacgca tcacaaccgc gcgctattta
    37921 ctatgtcgtt gcgctgcaaa tctgggaata ttttagcttt tacggcatgc gtgccctgct
    37981 gattctctat ctcaccaatc aactaaaata caacgatact cacgcctacg agttatttag
    38041 cgcctactgt tcgctggtgt atgtcacgcc aatcctcggt ggctttttgg cggataaagt
    38101 tctcggcaat cgcatggcgg tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt
    38161 gctgggtgcc agtgagatcc atccgtcatt cctctatctg tccctggcga ttatcgtctg
    38221 cggctatggc ctgtttaaat ctaacgtaag ctgtctgctc ggcgagctgt atgagccaac
    38281 cgatccgcgt cgtgatggcg gtttctcgct gatgtatgcg gcgggtaacg tggggtctat
    38341 tatcgcacct atcgcctgtg gttacgccca ggaagagtac agctgggcga tgggctttgg
    38401 cctggcggcg gttggcatga tcgcgggtct ggtcattttc ttatgtggca atcgtcattt
    38461 cactcatacc cgcggcgtta acaaaaaagt actgcgtgcg acaaactttc tcctgccgaa
    38521 ctggggatgg ctgctggttc tgctggtggc aacgcctgca ctgattaccg tgctgttctg
    38581 gaaagagtgg tcggtatacg ccttaattgt cgcgactatt attggcctgg gtgtactggc
    38641 aaaaatttat cgcaaagcag aaaaccagaa acagcggaag gagctggggc tgattgtgac
    38701 gctcacctta ttcagtatgt tgttctgggc cttcgcacaa cagggcggta gctcgattag
    38761 cctttatatc gaccgcttcg ttaaccgcga tatgtttggt tataccgttc cgaccgcgat
    38821 gttccagtcg attaatgcct tcgcagttat gctgtgcggt gtgttcctgg cgtgggtggt
    38881 taaagaaagc gtcgcaggta atcgtaccgt gcgcatctgg ggaaaatttg ctcttggcct
    38941 tggcctgatg agcgccggat tctgcattct gaccttaagc gcccgctggt ccgcaatgta
    39001 tggtcactct tctctgccac tgatggtgtt aggcctcgcg gtgatgggct ttgcggaact
    39061 gtttatcgac ccggttgcca tgtcgcaaat tacgcgcatt gaaattccgg gcgtgaccgg
    39121 cgtattaacc ggcatctaca tgctgctttc tggcgcgatc gcgaactatc tggcgggcgt
    39181 gattgccgat cagacatcgc aggcttcgtt tgatgcttcc ggggcgatca actactccat
    39241 caatgcatat attgaagtat ttgatcaaat tacctggggc gcactggcgt gtgtaggact
    39301 ggtactgatg atttggctgt atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga
    39361 gtcataatag gccagcaccg atgcctgatg cgacgcttga cgcgtcttat caggcctacg
    39421 tatttcctgc aatttattga atttgcacaa atttgtaggc tggattaggc gttcacgccg
    39481 catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc gcgccgtctc
    39541 atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt gcggataatc
    39601 cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgctt tccctggtac
    39661 atcgcgcagc tccggtaacc atcgacgaat aaactcgccc tcacggtcaa atttctcgcc
    39721 ctgagttata ggattgaaaa tacgaaaata cggcgcagca tcagtacccg tagaagccgc
    39781 ccactgccag ccaccgttat tagctgccaa atcaccatca atcagctgcg acataaaata
    39841 tcgctcgcct tcgcgccagt cgatcaacaa atctttcacc agaaaactgg ctgtaatcat
    39901 ccgtagccgg ttatgcatcc agccagtgct gttaagctga cgcatagcgg catcaataat
    39961 cgggtatccc gttttgcctt tctgccaggc ctgtaaatgt gcgggattgc tttgccactg
    40021 cacacgatcc gtccaggcaa taaatggacg atgtttacac aacgaggggt aatacgtcat
    40081 cagatgacgg tagaactcgc gccagatcag ctcactaagc cagacactac cggccccacc
    40141 gtccagcgcc tgcggctgtt ccgccaacaa gcgatgcaaa cactggcgag gcgataaccc
    40201 gcccgttgcc aagctggcgg acaaacggct ggtgccttcc actgccggaa aatctcgttg
    40261 ttgctcatac tctccagcac cgttctcgca aaactggcgt aattgcgcaa tcgccgcttt
    40321 ttcttccacc gggaaatgcg cagtatcgaa agactgacga ggataattca gcgtaatgga
    40381 tggcgcgggc tttatcgatc cgctactacg aacttttggt gcagcgacgc actccggcat
    40441 cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt tgtacatctc
    40501 gtgattgccg gtcatcaccg cccccggcgg caggatcacg ctgtcatcaa atccttcaca
    40561 caccacgtta cgcagcgctc tttcaacgtc cacatcccgc gctcgctcat tcacctcata
    40621 ctgatagtta taaaaaagat gagtaacgct gttttccgcg cacacctgtt taactatttc
    40681 gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt tttccgccag
    40741 cgcgatttgt agcccattca gttgagcatt gataagttca gcctgacgcg gcgacatgtt
    40801 atgcgtctcc cactggcgtg gtgtagcgat atacagcgcc agcacgc
//
LOCUS       DADLTF010000018        38753 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.018, whole genome shotgun sequence.
ACCESSION   DADLTF010000018 DADLTF010000000
VERSION     DADLTF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.018"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JN749_000925"
     CDS             complement(<1..246)
                     /gene="yefM"
                     /locus_tag="JN749_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HAY7367423.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            529..579
                     /gene="hisL"
                     /locus_tag="JN749_000926"
     CDS             529..579
                     /gene="hisL"
                     /locus_tag="JN749_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HAY7367424.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            725..1624
                     /gene="hisG"
                     /locus_tag="JN749_000927"
     CDS             725..1624
                     /gene="hisG"
                     /locus_tag="JN749_000927"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HAY7367425.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1630..2934
                     /gene="hisD"
                     /locus_tag="JN749_000928"
     CDS             1630..2934
                     /gene="hisD"
                     /locus_tag="JN749_000928"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HAY7367426.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2931..4001
                     /gene="hisC"
                     /locus_tag="JN749_000929"
     CDS             2931..4001
                     /gene="hisC"
                     /locus_tag="JN749_000929"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HAY7367427.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4001..5068
                     /gene="hisB"
                     /locus_tag="JN749_000930"
     CDS             4001..5068
                     /gene="hisB"
                     /locus_tag="JN749_000930"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HAY7367428.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5068..5658
                     /gene="hisH"
                     /locus_tag="JN749_000931"
     CDS             5068..5658
                     /gene="hisH"
                     /locus_tag="JN749_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HAY7367429.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5658..6395
                     /gene="hisA"
                     /locus_tag="JN749_000932"
     CDS             5658..6395
                     /gene="hisA"
                     /locus_tag="JN749_000932"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HAY7367430.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6377..7153
                     /gene="hisF"
                     /locus_tag="JN749_000933"
     CDS             6377..7153
                     /gene="hisF"
                     /locus_tag="JN749_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HAY7367431.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7147..7758
                     /locus_tag="JN749_000934"
     CDS             7147..7758
                     /locus_tag="JN749_000934"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HAY7367432.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="JN749_000935"
     CDS             complement(7944..8921)
                     /gene="wzzB"
                     /locus_tag="JN749_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HAY7367433.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="JN749_000936"
     CDS             complement(9067..10233)
                     /gene="ugd"
                     /locus_tag="JN749_000936"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HAY7367434.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEDYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="JN749_000937"
     CDS             complement(10483..11889)
                     /gene="gndA"
                     /locus_tag="JN749_000937"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAY7367435.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11998..12357)
                     /locus_tag="JN749_000938"
     CDS             complement(11998..12357)
                     /locus_tag="JN749_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367436.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12354..12906)
                     /locus_tag="JN749_000939"
                     /pseudo
     CDS             complement(12354..12906)
                     /locus_tag="JN749_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13007..13606)
                     /locus_tag="JN749_000940"
     CDS             complement(13007..13606)
                     /locus_tag="JN749_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367437.1"
                     /translation="MSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLA
                     INNIEFSTLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYL
                     RFPLFPQNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIAT
                     ILLFTINPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13778..14716)
                     /locus_tag="JN749_000941"
                     /pseudo
     CDS             complement(13778..14716)
                     /locus_tag="JN749_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14779..15153)
                     /locus_tag="JN749_000942"
     CDS             complement(14779..15153)
                     /locus_tag="JN749_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7367438.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFELLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JN749_000943"
     CDS             complement(15157..16305)
                     /gene="wzy"
                     /locus_tag="JN749_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HAY7367439.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFLCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16325..17194)
                     /locus_tag="JN749_000944"
     CDS             complement(16325..17194)
                     /locus_tag="JN749_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7367440.1"
                     /translation="MATYNGECWIEEQLKSIIEQKDVDISIFISDDLSTDNTLNICEE
                     FQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKNAI
                     DCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETLIE
                     FKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANISFK
                     AKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKKAD
                     KIYILLMLILNIF"
     gene            complement(17223..18113)
                     /locus_tag="JN749_000945"
     CDS             complement(17223..18113)
                     /locus_tag="JN749_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HAY7367441.1"
                     /translation="MNSNIYAVIVTYNPELKNLNELITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JN749_000946"
     CDS             complement(18143..19399)
                     /gene="wzx"
                     /locus_tag="JN749_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HAY7367442.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JN749_000947"
     CDS             complement(19415..19960)
                     /gene="rfbC"
                     /locus_tag="JN749_000947"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAY7367443.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JN749_000948"
     CDS             complement(19965..20843)
                     /gene="rfbA"
                     /locus_tag="JN749_000948"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7367444.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JN749_000949"
     CDS             complement(20902..21801)
                     /gene="rfbD"
                     /locus_tag="JN749_000949"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAY7367445.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JN749_000950"
     CDS             complement(21801..22886)
                     /gene="rfbB"
                     /locus_tag="JN749_000950"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7367446.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JN749_000951"
     CDS             complement(23259..24152)
                     /gene="galF"
                     /locus_tag="JN749_000951"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HAY7367447.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JN749_000952"
     CDS             complement(24327..25721)
                     /gene="wcaM"
                     /locus_tag="JN749_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HAY7367448.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JN749_000953"
     CDS             complement(25732..26952)
                     /gene="wcaL"
                     /locus_tag="JN749_000953"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HAY7367449.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSYEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JN749_000954"
     CDS             complement(26949..28229)
                     /gene="wcaK"
                     /locus_tag="JN749_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HAY7367450.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRSYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMIADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JN749_000955"
     CDS             complement(28409..29887)
                     /gene="wzxC"
                     /locus_tag="JN749_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HAY7367451.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVSVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JN749_000956"
     CDS             complement(29889..31283)
                     /gene="wcaJ"
                     /locus_tag="JN749_000956"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HAY7367452.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDTKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JN749_000957"
     CDS             complement(31338..32708)
                     /gene="cpsG"
                     /locus_tag="JN749_000957"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HAY7367453.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JN749_000958"
     CDS             complement(32813..34249)
                     /gene="cpsB"
                     /locus_tag="JN749_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HAY7367454.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASSEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGHHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="JN749_000959"
     CDS             complement(34252..35475)
                     /gene="wcaI"
                     /locus_tag="JN749_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HAY7367455.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLLFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35472..35951)
                     /locus_tag="JN749_000960"
     CDS             complement(35472..35951)
                     /locus_tag="JN749_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HAY7367456.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="JN749_000961"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35951..36919)
                     /gene="fcl"
                     /locus_tag="JN749_000961"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAY7367457.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="JN749_000962"
     CDS             complement(36922..38043)
                     /gene="gmd"
                     /locus_tag="JN749_000962"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAY7367458.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="JN749_000963"
     CDS             complement(38069..38617)
                     /gene="wcaF"
                     /locus_tag="JN749_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HAY7367459.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38633..>38753)
                     /locus_tag="JN749_000964"
     CDS             complement(38633..>38753)
                     /locus_tag="JN749_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyl transferase"
                     /protein_id="HAY7367460.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10349 a         9687 c         8721 g         9996 t
ORIGIN      
        1 aataatgtcc ttttccgttc ctttgcctga tttcaggcta tcgattgagt ccatcaatct
       61 ccgggcgtta gcgggggaac gcagtagata agccgtctct tccagcgagt tgtattcttc
      121 gagtgacatc agaacacaag cctctccatt ctgacgagta atgaggatcg gggcatgatc
      181 ttcaacggct ttcatcattg ttgccgacaa attctgacgc gcttcgctgt agctaattgt
      241 acgcatgtaa atctcctatt ttgtacagtt cattgtacaa tgataggtgt taattaacta
      301 tttattaatt agtttgtaga tcaagacatt gccagcgaga cgaaaaatca gactttgctc
      361 tttttggtgc tgtcaagtta gaggacagtc ctcttagccc cctcctttcc ccgctcattc
      421 attaaacaaa tccatcgcca taaaatatat aaaaaagccc ttgctttcta acgtgaaagt
      481 ggtttaggtt aaaagacatc agttgaataa acattcacag agatttttat gacacgcgtt
      541 caatttaaac accaccatca tcaccatcat cctgactagt ctttcaggcg atgtgtgctg
      601 gaagacattc agatcttcca gtggtgcatg aacgcatgag aaagcccccg gaagatcacc
      661 ttccgggggc ttttttattg cgcggttgtt aacggttcag acaggtttaa agaggaataa
      721 gaaaatgaca gacaacactc gtttacgcat agctatgcag aaatccggcc gtttaagtga
      781 tgactcacgc gaattactgg cgcgctgtgg cattaaaatc aatcttcaca cccagcgcct
      841 gatcgcgatg gcagaaaaca tgccgattga tattctgcgc gtgcgtgacg atgacattcc
      901 cggtctggta atggacggcg tggtagacct tgggattatc ggcgaaaacg tgctggaaga
      961 agagctgctt aaccgccgcg cccagggtga agatccgcgc tactttaccc tgcgtcgtct
     1021 ggatttcggc ggctgccgcc tttcgctggc aacgccggtt gatgaagcct gggacggtcc
     1081 gctctcctta aacggtaaac gtatcgccac ctcttatcct cacctgctca agcgttatct
     1141 cgaccagaaa ggtatctctt ttaaatcctg cttactgaac ggttctgtcg aagttgcgcc
     1201 gcgtgccggg ctggcggatg cgatttgcga tctggtttcc accggtgcca cgctggaagc
     1261 taacggcctg cgcgaagtcg aagttatcta ccgctcgaaa gcctgcctga tccagcgcga
     1321 tggcgaaatg gaagaatcta aacagcaact gatcgacaag ctactgaccc gtattcaggg
     1381 tgtgatccag gcgcgcgaat caaaatacat catgatgcac gcaccgaccg aacgcctaga
     1441 tgaagtcatc gccctgctgc caggtgccga acgcccaacc attctgccgc tggcgggtga
     1501 tcaacagcgc gtagcgatgc acatggtgag tagcgaaacc ctgttctggg aaacgatgga
     1561 aaaactgaaa gcactgggtg ccagttcaat cctggtcctg ccgattgaga agatgatgga
     1621 gtgatcgcca tgagctttaa cacaatcatt gactggaata gctgtactgc ggagcaacaa
     1681 cgccagctgt taatgcgccc ggcgatctcc gcttctgaaa gcattacccg caccgttaac
     1741 gatattctcg ataacgtgaa aacgcgtggc gatgaggccc tgcgtgaata cagcgcgaag
     1801 tttgataaaa ccacggttac cgcgctgaag gtgtctgctg atgagatcgc cgccgccagc
     1861 gaacgcctga gcgacgagct aaaacaggcg atggcggtgg cagtaaagag tattgaaacc
     1921 ttccacactg cgcaaaaact gccgccggta gatgtagaaa cgcagccagg cgtgcgttgc
     1981 cagcaggtca cgcgcccggt ggcttcagtg ggtttgtata ttccgggcgg ctccgccccg
     2041 ctcttctcaa cggtattaat gctggcaact ccggcgcgta ttgcgggctg taaaaaagtg
     2101 gtgctgtgct caccgccgcc gattgccgat gaaattcttt atgcggcgca gctgtgcgat
     2161 gtgcaggacg tgtttaacgt cggcggcgca caggccattg ccgcgctggc gtttggtacg
     2221 gaatctgtgc cgaaagtgga caaaatcttc gggccgggta acgcctttgt caccgaagca
     2281 aaacgccagg taagccagcg tctggacggt gcggcgatcg atatgcccgc aggcccgtcg
     2341 gaagtgctgg tgattgctga cagcggcgct acgccggatt ttgtggcttc tgatttgctt
     2401 tctcaggctg aacacggccc tgactcacag gtaattttac tgacccccga tgctgatatg
     2461 gcgcgtcgcg ttgccgaggc cgtcgaacgc caactggcag aactgccgcg tgccgaaacc
     2521 acccgccagg cactgaacgc cagccgcctg atcgtgacta aagatttagc gcagtgcgtg
     2581 gagatctcca accagtacgg cccggagcac ctgatcattc agacccgcaa cgcccgcgaa
     2641 ctggtcgatg gcatcaccag cgccggttcg gtatttcttg gtgactggtc accggaatcg
     2701 gcaggcgact acgcctccgg caccaaccac gtgctgccga cttacggtta caccgccacc
     2761 tgttccagcc tcgggctggc agatttccag aaacgcatga ccgtacagga actgtcgaaa
     2821 gaaggtttct ccgcgctggc ttcgaccatt gaaacactgg ccgccgccga gcgcctgacc
     2881 gcccacaaaa atgccgttac tttgcgtgtt aacgtcctta aggagcaagc atgagcaccg
     2941 tgactattac tgatttagcg cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc
     3001 gtcgtctggg cggtaacggc gacgtctggc tgaacgccaa cgaatatccc acagccgtgg
     3061 agtttaagct tactcagcaa acgctcaacc gctacccgga atgccagcca aaagcggtga
     3121 ttgaaaatta cgcgcaatat gcaggcgtaa aaccggaaca ggtgctggtc agccgtggtg
     3181 cggacgaagg tattgaactg ctgatccgcg ctttttgcga accgggtaaa gacgccatcc
     3241 tctactgccc gccaacgtac ggcatgtaca gcgtcagcgc tgaaaccatt ggtgtcgaat
     3301 gccgcacagt gccgacgctg gaaaactggc aactggactt acagggcatt tccgacaagc
     3361 tggacggcgt aaaagtggtc tatgtttgca gccccaacaa ccccaccggg caactgatca
     3421 atccgcagga ttttcgcacc ctgctggagc taacccgcgg taaggcgatt gtggttgccg
     3481 atgaagctta tatcgagttt tgcccgcagg catcgttagc tggctggctg gcggaatatc
     3541 cgcacctggc gattttgcgt accctttcga aagccttcgc gttggcgggg cttcgttgcg
     3601 gatttacgct ggcaaacgaa gaagtcatca acctgctgat gaaagtgatc cccccctacc
     3661 cgctctcgac gccggttgcc gacattgccg cacaggcgtt aagcccgcag ggaatcgtcg
     3721 ccatgcgcga acgggtggcg caaattatta cagaacgcga atacctgatt gccgcactga
     3781 aagagatccc ctgcgtggag caggttttcg actctgaaac caactacatt ctggcgcgct
     3841 ttaaagcctc cagtgcagtg tttaaatctt tgtgggatca gggcattatc ttacgtgatc
     3901 agaataaaca accctcttta agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa
     3961 gccagcgcgt cattgacgcc ttacgtgcgg agcaagtttg atgagtcaga agtatctttt
     4021 tatcgatcgc gatggaaccc tgattagcga accgccgagt gattttcagg tggaccgttt
     4081 tgacaaactc gcctttgaac cgggcgtgat cccggaattg ctgaagctgc aaaaagaggg
     4141 ctacaagctg gtgatgatca ctaatcagga tggtctggga acacaaagtt tcccgcaagc
     4201 ggattttgat ggcccgcaca acctgatgat gcagatcttc acctcgcaag gcgtgcagtt
     4261 tgatgaagtg ctgatttgtc cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa
     4321 agtaaaactg gtggagggtt atctggctga gcaagcgatg gatcgtgcca acagttatgt
     4381 aattggcgat cgcgcgaccg acattcaact ggcggaaaac atgggcatta atggtttacg
     4441 ctacgaccgc gaaaccctga actggccaat gattggcgag caactcacta aacgagaccg
     4501 ttacgcccat gtagtgcgca acaccaaaga gacgcagatt gacgtccagg tgtggctgga
     4561 tcgcgaaggt ggcagcaaga ttaacaccgg cgttggcttc tttgatcaca tgctggatca
     4621 gatcgccacc cacggcggtt tccgcatgga aatcaatgtc aaaggcgacc tctatatcga
     4681 cgatcaccac accgtcgaag ataccggcct ggcgctgggc gaagcgttaa aaattgccct
     4741 cggcgacaaa cgcggtattt gccgctttgg ttttgtgctg ccgatggacg aatgccttgc
     4801 ccgctgcgca ctggatatct ctggtcgccc gcacctggaa tataaagccg agtttaccta
     4861 ccagcgtgtg ggcgatctca gcactgaaat gatcgaacac ttcttccgtt cgctctctta
     4921 caccatgggc gtgacactac acctgaaaac caaaggtaaa aacgatcatc accgtgtaga
     4981 aagcctgttc aaagcctttg gtcgtaccct gcgccaggcc atccgcgtcg aaggcgacac
     5041 cctgccctcg tcgaaaggag tgctgtaatg aacgtggtga tccttgatac tggctgcgcc
     5101 aacctgaact ctgtgaagtc tgccattgcg cgccacggtt atgaacccaa agtcagccgt
     5161 gacccggaca ttgtgttgct ggccgataaa ctgtttttac ccggcgtcgg caccgcacaa
     5221 gcagcgatgg atcaggtgcg tgagcgcgag ctgtttgatc tcatcaaagc ctgtacccaa
     5281 ccggtgctgg gtatctgctt agggatgcaa ctcctgggac gacgcagcga agagagcaat
     5341 ggcgtcgact tgctgggcat catcgacgaa gacgtgccga aaatgaccga ctttggtctg
     5401 ccactgccac atatgggctg gaaccgcgtt tatccgcagg caggcaaccg cctgtttcag
     5461 gggattgaag acggcgcgta cttttacttt gttcacagct acgcaatgcc agtcaatccg
     5521 tggaccatcg cccagtgtaa ttacggcgaa ccgttcaccg cggcggtaca aaaagataac
     5581 ttctacggcg tgcagttcca cccggagcgt tctggtgccg ctggcgctaa gttgctgaaa
     5641 aacttcctgg agatgtgatg attattccgg cattagattt aatcgacggc accgtggtgc
     5701 gtctccatca gggcgattac ggcaaacaac gcgattacgg taacgacccg ctgccgcgct
     5761 tacaggatta cgctgcgcaa ggtgccgaag tgttgcacct ggtggatctg accggggcaa
     5821 aagatccggc taagcgtcaa atcccgctga ttaaaaccct ggtcgcgggc gttaacgtcc
     5881 cggtgcaggt tggcggcggt gttcgtagtg aagaagacgt ggcggcgtta ctggaagctg
     5941 gcgttgcgcg cgtggtggtc ggttccaccg cagtgaaatc accggacgta gtgaaaggct
     6001 ggtttgaacg tttcggtgcc gatgcgctgg tgctggcact ggatgtccgt attgacgagc
     6061 aaggcaacaa gcaagtggca gtcagcggct ggcaagagaa ctcgggcatt tcactggaac
     6121 aactggtgga aacctatctg cccgtcggcc tgaaacatgt gttgtgtacc gatatctcgc
     6181 gcgacggcac gctggcggga tctaacgtct ctttatatga agaagtgtgt gccagatatc
     6241 cgcaagtggc gtttcagtcc tccggcggca ttggcgacat taacgatgtt gctgccctac
     6301 gtggcactgg cgtacgcggc gtaatagttg gtcgtgcatt actggaaggt aaattcaccg
     6361 tgaaggaggc catcgcatgc tggcaaaacg cataatcccg tgtctcgacg ttcgtgatgg
     6421 tcaggtggtg aaaggcgtac agtttcgcaa ccatgaaatc attggcgata tcgtgccact
     6481 ggcaaaacgc tacgccgaag aaggtgcaga tgaactggtg ttctacgata tcaccgcttc
     6541 cagcgatggc cgtgtggtag ataaaagctg ggtatctcgc gtggcggaag tgatcgacat
     6601 tccgttttgt gtggcgggtg ggattaagtc tctggaagat gccgcgaaaa ttctttcctt
     6661 tggcgcggat aaaatttcca tcaactcccc tgcgctggca gacccaacat taattactcg
     6721 cctggccgat cgctttggcg tgcagtgtat tgtggttggt attgatacct ggtacgacgc
     6781 cgaaaccggt aaatatcatg taaatcaata taccggcgat gaaagccgca cccgcgtcac
     6841 tcaatgggaa acgctcgact gggtagagga ggtgcaaaaa cgcggcgccg gagaaatcgt
     6901 cctcaacatg atgaatcagg acggcgtgtg taacggttac gacctcaagc agctgaaaaa
     6961 agtgcgtgaa gtttgccacg tcccgctgat tgcctccggt ggcgcgggca ccatggaaca
     7021 cttcctggaa gccttccgcg atgccgacgt tgacggtgcg ctggcagctt ccgtattcca
     7081 caaacaaata atcaatattg gcgaattaaa agcgtacctg gcaacacagg gcgtggagat
     7141 caggatatgt taacagaaca acaacgtcgc gaactggact gggaaaaaac cgacggtctg
     7201 atgccggtga ttgtgcaaca cgcggtatct ggcgaagtgt taatgctggg ctacatgaac
     7261 ccggaagcct tagacaaaac catcgaaagc ggtaaagtca ccttcttctc gcgcactaaa
     7321 cagcgactgt ggaccaaagg cgaaacctcg ggcaattttc tcaatgtggt aagtattgcc
     7381 ccggactgcg acaacgacac attactggtg ctggcgaatc ccatcgggcc aacctgccac
     7441 aaaggcacca gtagctgctt cggcaacacc gctcaccagt ggctgttcct gtatcaactg
     7501 gaacaactgc tcgccgaacg taaatatgcc gacccggaaa cctcctacac cgccaaactg
     7561 tatgccagcg gcactaaacg cattgcgcag aaagtgggcg aagaaggcgt ggaaaccgca
     7621 ctggcagcaa cggtacatga tcgctttgag ctgaccaacg aggcatctga cttgatgtat
     7681 cacctgctgg tgctgttgca ggatcaggat ctggatttaa cgacagtaat tgagaacctg
     7741 cataaacggc atcagtgagt tacgggctga gcaggtgtga tgttgttgtc acacccggag
     7801 ccattgcccg atgcgcaaac ttatcgtgcc tacacattat ctcaacacac gtaggccgga
     7861 taagatgcgc cagcatcgca tccggcaaaa aaaacgggca tggtgtcacc accctgcccg
     7921 tttctcttaa atgcacaata atattacttc gcgttgtaat tacgcagagc gttacgcccc
     7981 aacacaatcc ccgcgccaac catgccgccc agaagcactg ccagaatcaa ggtgattgcc
     8041 tttttcggac tatcgcgacg aataggtaac gtcggtttca tcacatagcg gtaagcatga
     8101 atatcaagat catcaaactt taatttttca atatccaaca ggttttgacg tgtctgatag
     8161 tagtttggtg agaacaccaa cggacgagtc gcttcatgct taatcatcga ctccagcgct
     8221 tcgctcccta gaaggaacaa cgtatcttgc gtcacatctt cagtctgctg aacctgtggc
     8281 tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
     8341 tctttctgct cctgcgcgac cacttcctgg gttcttaaag agtcctgcaa gtttttccgt
     8401 cccagagcaa tgttgtcctt gagatccttt tctagctctt gattcacttt atcatcaact
     8461 tgctgaatgt attgggccaa cttcatttgt gcgccctctg cagtttgccc aacataagaa
     8521 acagtcaatg gtaattgttg gttcttaaca gaaggttcga tggtaagttt ttctggctct
     8581 tcctgattat ccagcgtttc tgctaatgca gagaaggcag aactgaagcg accaattaac
     8641 gtctcctgca aatccgatac tttcggtgca gcctgaccat agataacatt catggcattg
     8701 ttatagccag caatttgccc cacgtcgggc tgagtgataa ttgctgttga cgtccatttc
     8761 tccttcgcta ctgccaaata accaatagcc agggcaatag ccacaatgac ggaaatgata
     8821 attgtcatct tgccacgcca caactgcact agtaaatcaa tcaaatcaat ctgttccggg
     8881 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aactgacgaa
     8941 aatttccaag tccgcatagt ttagcgataa tccccgtgaa tgctatagga taagtgataa
     9001 aaataatgaa ttattgcgga ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac
     9061 aagatgttag tcgctgccaa agagatcgcg ggtgtagacc ttatctgcca catccttaag
     9121 ctcttctgcc atacggttgg agataatgac gtcggcttgt tgtttgaagg tggcgagatc
     9181 acgttccagg cgagagttga agaatgagtc ttctttcatc accggctcgt agatgatcac
     9241 ttcaacgcct ttcgccttga tacgcttcat aatcccctga atagaagacg tacggaagtt
     9301 atctgacccg ctcttcataa tcagacgata aatacccacc acttgcggtt tgcgtgacaa
     9361 aatggcatcg gcaataaaat ctttacgcgt gcggtttgca tcgacaattg ccgagatcag
     9421 gttattcggc acagactggt agttcgccag taactgcttg gtatctttcg gcagacaata
     9481 accaccataa ccaaacgacg gattgttgta atggttgcca atacgcggat cgagacaaac
     9541 gccttcgata atctggcgag tattcagacc taaactttct gcataactat ccagttcatt
     9601 aaagtatgct acgcgcatcg ccagataggt attagcgaaa agtttaatcg cttctgcttc
     9661 agtggagtcg gtaaacaggg tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc
     9721 agcaaaacgt tctgcgcgtt ctgaacgctc accgatgaca atacgtgacg gatggagatt
     9781 atcgtaaagg gctttacctt cgcggaggaa ttccggagag aaaataatat tttcagtacg
     9841 atatttctta tgcatcgctg cggtaaaacc aacgggaacc gttgatttga tgaccataac
     9901 cgcataagga tttatctcaa ctacgtcttt aattactgat tctacactgg atgtattgaa
     9961 ataattagtt ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg
    10021 gtagtcttca tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg
    10081 ctgaatttcc ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg
    10141 taaaatatct aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc
    10201 tacatagcca gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg
    10261 tgaatcatga tgtttttagc atcgtgataa gatgctaaaa gttttaacgc tacgttgtca
    10321 gatggtcaac gcaacgaaaa acaattcaga ttttctttat aagaatagct gatgtttatt
    10381 aagttaatat taagcagtga aatttagtct ataagatatt tggcaaaaaa aagcccggtg
    10441 caatatacgc cgggcctcaa ttttattgtt ggttaaatca gattaatcca gccattcagt
    10501 atggaacaca ccttctttat caatgcgctt ataagtatgc gccccgaaat agtcacgctg
    10561 tgcctggatc aggttcgcag gcagaaccgc ggcacggtag ctatcgtaat aggcaaccgc
    10621 agcggcgaag gtcggaaccg ggataccgtt ctgtactgca taagcgacga catcgcgcag
    10681 cgcctgctgg tagtcatcgg caatttgctt gaagtacggt gccagcagca ggttagcgat
    10741 ctgcggattt tcggcataag catcggtgat tttctgcagg aactgcgcac ggatgatgca
    10801 gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccagttgt attcttcaga
    10861 cgcagcacgc agctgagaga agccctgagc gtaagaaacg attttgccca gatacagcgc
    10921 actgcgaact ttttcgatga actcagcctt gtcgcctgct gactgagctt gcggaccaga
    10981 gagaacttta gatgcggcaa cgcgctgatc tttcagagaa gagatataac gtgcaaacac
    11041 agactcggta atcagcgaca gcggttcgcc gagatccagc gcgctctggc tggtccattt
    11101 accggtgcct ttgtttgctg cttcatccag aatcacatca accaggtagt taccgtcttc
    11161 atcttttttg gtgaagatat ctttagtaat gtcgatcagg tagctgctaa gttcaccgtt
    11221 attccactcg gtgaaagtct gggccagttc ttcattagat agattcaggc cacctttgag
    11281 cagagaatac gcttcagcaa tgagctgcat atctccgtat tcaataccat tgtgaaccat
    11341 cttcacataa tggcctgcgc cgtcggcacc aatataagta acgcatggct cgccgtcttc
    11401 tgccacggca gcgattttag tcaggatagg cgcaaccagc tcataagcct ctttctgccc
    11461 gccaggcata atagaaggac ctttcagcgc gccctcttca ccgccggaaa caccggtacc
    11521 gatgaagtta aagccctctg ctgaaagctc acgattacga cgaatggtgt cctggaagaa
    11581 ggtattacca ccatcaatga tgatgtcacc tttatcgagg tatggtttca gggaatcaat
    11641 cgcagcatcc gtgcctgcac ctgctttcac cattaacagg atgcgacgag gcgtttccag
    11701 agattcaaca aactctttca ccgtatagta aggagccagt ttcttgcctg gattttcagc
    11761 aatcacttct tccgtctttt cgcgggagcg gttgaaaata gagacggtat aaccacgact
    11821 ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc cgatctgttg
    11881 ctttgacatt gtttactcct gtcttctata ttattaaccg tctattaacc gttatattat
    11941 taaccgtcgt gattttcatc ttctaatcaa ggaatacata tggttacagc atgcttctta
    12001 ctttatagcg ataagtatgc cattgggtgt atccataaga actttgaatt ttggatactt
    12061 ttctggatta aaattagcat agcttcgttt agatataaca ataaacgaac tattaaaaga
    12121 tttcatcttc tctattaaat catcctgtgg gattacctca cactcgggat tactaatagt
    12181 ccaatcacta tatcttgcta atccaaagac gtcaccaatt gccatccctt tatagaagta
    12241 aataccatcc tccaagccta attggataag attatctcca tataactcac tatattcact
    12301 tgctttcttc atcagttcat agctatagcg actttctaag cgtgaattaa aattcaagta
    12361 ttcagaataa aaatatttga attcaaaatg caacgatcgc cccgaaaaaa aaataataca
    12421 cacaaggata aaatatcgtc ccaacttgta atttgtaatg ttgatatctt ttattaaact
    12481 tgcaattgag atcgcaacca gcaatactga caatgggtaa atgggtgata aatacctgtc
    12541 accttgcgta acagaaaacc aaaataagac atatgttaaa acaatcgaga aaaataccca
    12601 aagtatttta ttattcctaa gtgtaaagac agacagtata gcataaaaaa caacatcact
    12661 gccaacttta ttaattgctt gttgcaaatc taatgtggca ggacttatac catgtcgagc
    12721 ttgttctgct gtttgaactt gtagatcaat ttcattccat aggtagtgac caaatatatt
    12781 tcctccagca ggactaaaag gatcaccaga gatccaaaaa ctacggacat accaataaat
    12841 gccaccaaga attgttgcca agctaaaaat aagaaatgtc tgtatgtttc tctttttcgc
    12901 tatcaatata acaaatatga tggctccata tatggcacca aatagtttta cgccacaagc
    12961 aattccaata aaaaaaccac ataaaaaaat taattcattt tgtgttttat tgttttctga
    13021 gcgaatatat ataagaatac tggctgcagc aaaacaaaga tagcgttaaa tcaatatatg
    13081 cataaccaag atgatcttta agtggattga tagtaaatag taggattgta gcaataatgc
    13141 taggataata agttttcgta atttcccatg aacagccaac aagtccacag gctattacaa
    13201 aaaaacagat atttgctaaa aactgagcta accttacatc tcctaactgt aaacctaaag
    13261 ccattaaaag atctgcattt tgcgggaaca gaggaaaacg caaatattgt tcaacaacaa
    13321 ttgcatggtg ctcaactaat gaacgagcca aaggtaagtg atacatagta tcatcccaat
    13381 caccgggagc tcgcagggac gataacaatg taaccacaaa aatcaaaaca agaaataaaa
    13441 taacaaacag cgtactgaac tcaatattat ttattgccag ccatctaaat tgctgacgtt
    13501 taataatgct taaaacccaa cacaccgtaa aaattaaaaa gatagcaatc ttattaaatt
    13561 gcccgaccat gcccagaaag taaattatca aaatatttaa agacatccct atgcataggc
    13621 aataaaaaaa taaagaaaat ttgttaagtc ctgccacccc ctccttaaaa aaagatgaca
    13681 atacaatatt accaatgaca aaaaatgtca ttccaaaagc aaaacaatac acaacataca
    13741 aatacaacgg caattcaatc atagtaaaat actctattta cattatcagt ttatagtttt
    13801 tttcagaaag ataaaataat gccttcattt cacgtctagt tgttgtattt gaatgcaaaa
    13861 taatgccaca tacaaatgcc attattgacg ataacatgat ggaggcagtt aaaaaggcag
    13921 tcggaaatct tctcaccaaa cctgtatcaa tataatcaac aatgatgggc aatcctaaaa
    13981 ttatagacaa agcagcaagt aaaacgccga taatactaaa aaagtatagt ggccgttcaa
    14041 gcgagtaaag tttaattatt gttttcagaa tcttgaaccc atcactccaa gttgatagtt
    14101 tgctcacact cccttcagat cgctcgccat attttgtatt aacctcacca tacttcatac
    14161 gtaattctaa agcatgaatt gtaagctcag tttcgatttc aaatcccctt gatagacatg
    14221 gaaaagattt aacatagcgt ctggaaaata cacgataccc cgacaacata tcagtaaaat
    14281 tccctttaaa gatagtctga acagtaccag tcaacagttt atttcctcag cgatgccctt
    14341 ttctgtatgt attttgatct ccattctctt gacgacatcc tacaaccata tccaattgat
    14401 tttttatgag atggttaatc atttctggag cagaacttgc atcataagtg ccatctccat
    14461 caaccattag ataaatatcc gcatctacat ctgagaacat acgtcttacg acattcccct
    14521 tgcctttcag aggaacagag tgaactgttg ctccagcatc ctctgccact tgtgctgtac
    14581 tgtcagtcga attattatca aatacatata tagaagcagt tggaatattc gttttgaaat
    14641 catttatgac ttttgctatt gccttctgct cattataaca aggaataatt acagcgatat
    14701 taaaagagtg atacataagc ccctcatata aactatttag ctaatgtgca agtaattgtg
    14761 tttttaacaa atcgtttact aacgaaaaat gaaatatttt gatgcgcaat atcctaaaac
    14821 caaactaaaa agagaaaagg tgaacaatgt aaatattgga gataaagcta agagttcaaa
    14881 taaaacgccg aataaataac tcatcgcccc cataaaaaat ataaatataa aatatctagc
    14941 cttggttgca gatactttaa atgagcatct tgcatttgca aaaaaagaaa aggttgctgc
    15001 acatataaaa ccaacaatgt tacttcggcc ttgaccaaaa gctaaattat tataacacgc
    15061 ataaaatacc ccccaatgta aagcagtatt gacaattcca attaaaaaat aactaaaaaa
    15121 agaactggtc aataacttcc ctatttttaa catcctttat tttgctccag aagtgaggtt
    15181 attactaatt tggatatttt ctatagaaaa tacccataaa actacaacgc ctataataaa
    15241 aattcccatg ggtgtaaaaa gaaatggatt agttatagaa acggtaatac tgaaaaaaag
    15301 tattaacgac atcattaatg atgtttttgt actatttaaa aaatagcctt tcaaaaagat
    15361 aagcaacata ggaactaatg atataaatac accaataacg cctgttttct gaagtatctc
    15421 aagaggtact atttcaatcc ttaatcgatc taatataaat gaaccaaatc ctcttccaaa
    15481 caagaacgtc gctaaatcaa catttttata ataaaaatat aaatcattaa atctaacaga
    15541 gtcagaatct tcactgcgtg atccgaccat gtaaagcata attataaata gaacgctgcc
    15601 caatattatt ataatagtat ttaaatatag cttatgtaat ttaaaactgg ctaataacag
    15661 tgataatgtt gtaaaaagta atacacctct tgattctgtc aataatatcg caagcactat
    15721 tatgagacat tttaaatatt ttttgtcgac aaaactgata atcgcaccga caccaaaaaa
    15781 gtagaaggat ttggaaaaaa aagccccatc gggacgaaaa aagaactcgc tagtcaatga
    15841 taagtgttca taaattaaat taaaatttaa caaaccgaaa ttcaacaaaa ttatgtatga
    15901 aatatataac agtgatccat ataatgcaaa tgcattaact attttaataa aaatctcaca
    15961 taaaaatact tttaaagtat atcttagtgt aaatagatat ataagtgaca gcataaaaag
    16021 gtatggttgc acatcaaaca gtatatcatt tattttatta ccagctaata agccgacaaa
    16081 gaagccataa aaaggaaata aaagcaacca tataaaaaga ctgacacatt tttgggtgat
    16141 tgaaaaattg aatgtaacta aattaaaaat taaaagtata aaactaaata caaataataa
    16201 atatcgcaaa gggaatattc ccaatggctc cagtaatctt ccaccaccac caataatcag
    16261 ttcaatacat aaaaatgtta taaaaatttt atttatatta ttcatattcg taaggtgatg
    16321 ttttttaaaa tatgtttaaa atcaacatta ataaaatata aattttatca gcctttttcc
    16381 gccttaactt ccaaaaactt aaagccaaga tcaaattgcc tatataacct ttacccaatt
    16441 gattattcac aaagctatca tcagctaagg cattagctat tttagttact tcttttcgat
    16501 accacttgtt tctcaccaaa ccaagccgtt tgtattttgc tttaaaacta atgtttgctc
    16561 ccacttggtt gttttcatgc tgtctgtaca gcattgttgg ttttctatca atataccacg
    16621 aataatttct cgtccgcgca aatgaatata aaaaccaatc atgcagacag atatcttgtg
    16681 cagcattttt attatttatg atgaacttct taaattcaat aagcgtttct ttttttataa
    16741 catatgtaca ccccggacct gctgcttcaa aaaaataatc gaattgagtt tgcgactgag
    16801 ccttatcaac taaatttttt tgccctgatg gataatatgc aattacattg ctggaataac
    16861 aattcgcatt attaaatacc aagcagtcaa ttgcattttt tattttatct ttataccaaa
    16921 tatcgtcctg atcactaaaa cagatataat catagttttc aagatcgaca tctttaatta
    16981 atctataaaa atttttacct gcccccccaa acttattgac gcttggaagt atatttataa
    17041 ttgagggata tgataattga aattcttcac aaatatttaa tgtattatca gttgataaat
    17101 catctgatat aaaaattgag atgtctacat ctttttgttc aataatactt tttaattgtt
    17161 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
    17221 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
    17281 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
    17341 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
    17401 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
    17461 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
    17521 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
    17581 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
    17641 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
    17701 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
    17761 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
    17821 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
    17881 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
    17941 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
    18001 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag ttcattcaga
    18061 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
    18121 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
    18181 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
    18241 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
    18301 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
    18361 ccagcgcatt aaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
    18421 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
    18481 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
    18541 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
    18601 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
    18661 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
    18721 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
    18781 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
    18841 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
    18901 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
    18961 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
    19021 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
    19081 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
    19141 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
    19201 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
    19261 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
    19321 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
    19381 tattttttat tatgctcatt attacatttc tttatcatat aaaataatca gcatctacaa
    19441 atttttgacc attaatatct ttatcagaaa gcaatggatt ttgaaccggc cattctatat
    19501 ttatactttt gtcgctccat aaaatcccac gctcttgcat ttttgaatag aaattatttg
    19561 ttttgtagac aaattctgct tcatcactaa gaacgcaaaa cccatgagcg aatccttccg
    19621 gtatccataa ttgacgctta ttctccgccg acaaatttac accaacccac ttgccaaatg
    19681 ttggtgatga tttccgaata tcaacagcta cgtcaaaaac ttcaccgaca acacagcgta
    19741 ccagttttcc ttgagcataa ggttccagct gatagtgcag tccgcgtaac acacccttag
    19801 ttgatttaga atggttatcc tgaacaaact caacctttcg tcctacagcc tcttcgaaaa
    19861 ctttctgatt gaagctttcc ataaaaaagc cccgctcatc accaaatact tttggttcaa
    19921 aaattagcac atcaggaatt tcagttttaa ttacgttcat tttattaata acctttaatc
    19981 attttcagca gatactgacc ataagcattt ttcttcagcg gctcggctaa taccttcact
    20041 tgctcagcat caataaagcc tttacggtaa gcaatttctt ccgggcagga aactttcagc
    20101 ccctgacgct cttcaattgt tgcaatgaag ttgcttgctt caataagact ttgatgtgtc
    20161 cccgtgtcca gccatgcata accacgcccc atcatagcga cagacaaacg tccttggtcc
    20221 atataaatac ggttaatatc ggtaatttcc agttcgccac gtgcagatgg tttaaggttt
    20281 ttcgccattt ctacaacgtc attgtcatag aaataaagcc cagtaaccgc atagttactt
    20341 tttggttcca gcggtttttc ttccagacta attgccgtac cgttatcatc aaattccacc
    20401 acaccgtagc gctcaggatc atttacgtga taggcaaaaa ccgttgcgcc actctctctg
    20461 ttaacagcgg tatccattaa tttcggcagg tcgtgaccat agaagatatt atcaccaagt
    20521 accagcgcac aatcatcacc acctataaat tcttcaccaa tgataaatgc ttgcgccaga
    20581 ccatccggac tcggttgtac tttatattgc agattaagcc cccactggct tccatcacca
    20641 agcaattgtt gaaaacgtgg tgtatcctgc ggcgtactga taatcagaat atctcgaata
    20701 cccgccaaca taagcgttga aagcggataa taaatcatcg gtttatcata aatcggtagc
    20761 agttgtttgc tcacagccat tgtcacagga taaagacgag tgccggaacc accagccaga
    20821 ataatacctt tacgcgtttt catttcacta ttccttttaa ttcatctcgc tctggcatca
    20881 tgagcgagat gcaataattt gttaaattgc cgtggtcgta aataattcgt tgagcatacg
    20941 tttcacgccc acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttcggt
    21001 attgaggcga gaattatggg gacgacgtgc tggtgtggga taggccgttg tcggcacggc
    21061 attaagtttg ttaagagcaa ggttgatccc cgctctgcgc gcttcttcaa ataccagagc
    21121 ggcataatcg tgccaggttg ttgtaccacc agcaaccaga tggtacaaac cagcaacttc
    21181 tggtttattt gcggccacgc gaatggcatg agcggtgcaa tcagccagta attcagcccc
    21241 cgttggtgcg ccgaattgat cgtttattac agccaactct tcgcgttctt ttgccaggcg
    21301 caacatcgtt ttggcaaagt tatttccttt accagcgtat acccagctgg tacggaaaat
    21361 aagatgcttt gcgcaatgtt cttgtaacgc tttttcccca gctagcttgg tttcaccgta
    21421 gacatttagc ggtgcggttg catccgtttc cagccatggc gtgtcaccgt tgcctgggaa
    21481 aacataatca gtggagtagt gtataaccca ggctccgact tcattagctg cttttgcaat
    21541 cgcttcgacg catgtcgcgt taagtaattg cgcgaaattc ggttctgatt ccgctttgtc
    21601 tactgcagtg tgagcagcag cattaacaat aacatcgggg cgaatttttt tgacggtttc
    21661 agccacacct tcggggttgc tgaaatctcc acaataatca gtggagtgaa catcgagcgc
    21721 aatcaagtta cccagaggag ccagagcacg ctgcagttcc caacccacct gccctgtttt
    21781 gccgaaaagc aggatattca ttactgacgg ccctcatagt tttgttcaat ccacgactga
    21841 taagcaccgc ttttcacatt ctcaacccaa tttgtattag ccagatacca ttccaccgtt
    21901 ttacgaatcc cgctctcaaa cgtttcttgc ggtttccagc ccaattcgcg gctaatttta
    21961 tcggcatcaa tggcgtaacg gcggtcatgc cccgggcgat cggcaacata agtaatttgc
    22021 tcacgataag atttctcttt cggtacaatc tcatccaaca aatcacaaat agtgagcact
    22081 acgtcgatgt ttttcttttc gttatgtcca ccaatgttat aagtttcacc cgctttacct
    22141 tcggttacga cggtatataa cgcacgagca tgatcctcta catacaacca gtcgcgaatt
    22201 tgatcccctt taccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat
    22261 ggaataagtt tttccgggaa gtgataagga ccataattgt tcgagcaatt agtcacaatg
    22321 gttggtaaac cataagtccg cttccaggca cgtaccaaat gatcgctgga agctttagac
    22381 gcagaatacg ggctacttgg cgcgtaagct gtcgtttccg taaatagcgg taacgcttcg
    22441 ttattatttg cttcatccgg atggggtaag tcaccataaa cttcatcagt agaaatatga
    22501 tgaaagcgga aacttttttt cttttcatca ttcagagcag accagtaatt gcgcgccgct
    22561 tctaaaagaa cataagtccc cacaatattg gtttcaataa atgccgcagg gcccgttatt
    22621 gaacggtcaa cgtggctctc tgccgccagg tgcatcaccg cgtctggctg gtgctgtgcg
    22681 aaaatacgcg acatggctac agcatcgcag atatctgcat gctcaaaagc ataacgttca
    22741 gaatcagaaa catcagcgag tgattctagg ttcccggcgt atgttaattt atcgacatta
    22801 acaacactat cttgcgtatt atttattatg tgacgaacaa cagcagaacc aataaaccct
    22861 gcgccaccag taacaagtat cttcactttt ttattccata tagctataga gcatgctgtg
    22921 aaatagactg ctccagattt gattaataga ggtattaata cacgctaccg cccctggctt
    22981 aacagctacc agtgcactgc atgcatctca acgatgtggc gagcgttagc cactcgcgcc
    23041 aaatccgaaa aattcaaacg ttaattgtct taccaaacag ccgtagaaac aaggaaaatc
    23101 ctggaaaaat ttgaataata tgagctatgt aacttcttgt ttttataggc tttataaaaa
    23161 cggcaacggc aggaaaattc aatgagcaaa acttatgcca cgaatcttca ctgccgttat
    23221 aattttctta tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca
    23281 atacctttac ggaacttcgc cccttctttc aggttgcgta ggccatactt cacaaacgcc
    23341 tgcatatagc ccattttttt gccgcagtcg taactgtcgc cggtcatcag cattgcatca
    23401 acggattgtt ttttcgccag ctcggcaata gcatcagtca gctgaatacg tccccatgca
    23461 ccaggctgag tacgttccag ttccggccaa atatcggcag aaagcacata gcgacctacg
    23521 gccatgatgt ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaata
    23581 cggctgactt taccctcacg gtccagcggc tctttagtct ggatgacgga gtattcagag
    23641 aggtcacccg gcatacgttt tgccagcacc tggctgcggc ccgtttcgtt gaaacgtgca
    23701 atcatggcag caaggttgta acgtagcggg tcggcgctgg catcgtcgat cacaacgtct
    23761 ggcagtacca cgacaaatgg gttgtcacca atggcaggtc gcgcacacaa aatggagtgg
    23821 cctaaaccta aaggttcgcc ctgacgcacg ttcataatgg tcacgcccgg cggacagatg
    23881 gactgtactt ccgccagcag ttgacgcttc acgcgctgct caaggagtga ttctaactca
    23941 taagaggtgt cgaagtggtt ttcgaccgcg ttcttggacg cgtgagttac caggaggatt
    24001 tctttgatcc ctgcagccac aatctcgtca acaatgtact gaatcattgg tttgtcgacg
    24061 attggtagca tctctttggg tatcgcctta gtggcaggca acatatgcat cccgagaccc
    24121 gctacaggaa taactgcttt taaattcgtc attatttcat ccacctgtaa aatggttgct
    24181 gaattatagc ttgttcgatt tttttcgcca gcatcaatta ccctgaattg attactgaat
    24241 tacttgtgat gttacgccgc ttcgttgtgg attgcagtag cattgttcct aagtatggct
    24301 ccatttttcc aggaatggtc gcaaatctac tccctccgtt ccggcaatct aaagttaatc
    24361 ttttccacat taacaatatg gtgattaatc ctgtcgatat cgacggagct ttgccctctt
    24421 tcattcaccg catgaacatt tgcaagagac agcagtgttt cttttttcgc cataaagacg
    24481 ccgcgaacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    24541 tgcatcacat tgatatttcg tataaaaaga tgttgcggct tgttgtgtaa ctccagcgag
    24601 gcacgcttca tctcaatgtt agttagcgac acaaaggaga cggcattccc ggcggagatt
    24661 tggatgccgc gcaatttata agcaagatgg gtattatcca gttgaatatt attcactcgg
    24721 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    24781 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcgact
    24841 gtcgcgttat caatgcccgc tttcttactg aaatccggcg tgatattgcg ggcattgata
    24901 ttacgaataa caaaatgttt accattttca acatggttca actgccgaca atccgatccc
    24961 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt gttatcataa
    25021 gtgcttcccg caaggcctat gccgatgccc cagttgattt tgccgttggt acagttgata
    25081 cgctcgatga catggtcaga tatcaaaata tcactgtcgt taattgccac gttccattca
    25141 atggcgtcgc cctgtaagtc gctgaactta cagttggtaa tgttggcacc gataatctgg
    25201 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    25261 gtcaggttgc gcatgacccg tttgtttttg ccgccaatat aaatctgcat taccgggcca
    25321 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc cagggtaatg
    25381 ttatgcatac cgccgccctc tccccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    25441 cctctgccgt tgccgcgcag gcttccaagg atgtgtaacg ttttgccggg agggatgaag
    25501 atgccggtgt tgatattgtc acaaacgaat ccggcaggca cgacgaccgt ttgcccttcg
    25561 ctgaaggctt gtttaaatga ggcgatccag tcatgtgggt tgtagtcgtt aatgttaacg
    25621 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    25681 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tacgttctcc tctataaagc
    25741 ctgcagcaag ctggcgagtt ctcgattaat cacctgctgg ttaaaatcgt gttcgacttt
    25801 ttcgcgcgca cgtttgacga ccggagccag ttcgtcggta tccagttggc taaacgccgc
    25861 cagacgttgt gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttatcggc
    25921 ctccaccagt tcaggaattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    25981 cgcttccatt agcgccaccg gaatgccttc catatcgcca tccgcgcccg ttaccgatgg
    26041 caacaggaag acatccgcgt cgtcgagcat ctccttcact tcatagctcg gtttaaagcc
    26101 cggcatctct accacatctt ccagttgata ttgttcgatg agggtgcgca ggcgtcgttc
    26161 ccacaggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    26221 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cagatatgat
    26281 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    26341 catacgcgat acggcgattt tttctctggg gcagcccatt ttttgcagcc ttccggccca
    26401 cagatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    26461 gtagtggttg agcacttccc gactggagat atcaataccg tggaagatgg tggcaatttt
    26521 gccgcgaatg atacccagtt cgcgtagttt tgctgcggtt acccccgccg ggccaaaatg
    26581 agcgatgaac acatcggcgc gaaacggtgt tgcgacctga ccacaaatgg cagacaaaat
    26641 caggttccgc gactcggcac catagcgttt gaggttgagc gcatgccagg tatttttacg
    26701 atgaatgcca cgtaaggtct ggctggcgcg gtggcgcagt ttcgctactt tgcctgcagg
    26761 ttcgtcctgt aaccagcggg ttctggcgac aaggttgtat ttcgtccatg ccgcgtgggt
    26821 gttctctgta tcgccttttt gcaaagcgac aatctccact tcaaagccca tatcaataaa
    26881 cgcggtaatt tgattgagga cgaaggtttc cgacgacagc gggaatttca ataagaagaa
    26941 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattccc
    27001 gtctgacgtt cgcgactgac ggcttcgtta aatcgagcgt tcagcaccgg aagctggcct
    27061 aaggtatccg caatcatcgc ttgcaggctg ccgtccagta aatgacggat atcaatcgcc
    27121 atctctggca gtcccagctg ctgcataatc ccggcggatt tatgttcata gttgatggca
    27181 atcgccggag tagcaaagtt cattgagata atggcggagt gcaggcgcgt accgacggtg
    27241 agttcacagg ctccgagaat tttgcccatt tccagatcgt tgagttcatc catcactacg
    27301 tggtaacggg caggatcgct gatgtgctgg cgcagattga gcgccaccat gcggtcgtct
    27361 ttgttatagc tgtcgatacc cgtacaggtg gagagcgcaa tcacctgata gccttcatcg
    27421 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgaga
    27481 cgtttgtcga acggtgccag ttcgcgcagg gtaattgcca ctgttttctg ttgtgcggca
    27541 acgtccagcc agtgttgaac ggcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    27601 agccacgcgg tatcgacgcc atgttccact tttgcggtgg tgatattgct gcgtttcatc
    27661 aaatcaaggc tgaccgattc gcgcaggatc agcgcgtcgc agtgaccaaa gacatagttc
    27721 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    27781 ggctttttcg ccataaatgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    27841 acaaaaaacg atccgccgac ctgaataatg gcgtcgtaac ctgacagcag gcgcacgaag
    27901 tcggtgaatc cctgggcgat ggcgatattg cgcagcttgc cagtgtcggt gacgcgtgag
    27961 agcaatacct gatgctggta gctgcgacgg aggacttttt taacgcgccc gacaacgccc
    28021 gccgcgctat tgtgttgtgt catctgcagg aacagcggat cgcccattac cgggcggttg
    28081 agcagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggt atgtggattg
    28141 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    28201 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaggtgagg
    28261 ctgtgtttgt tgtgttcgtt gccgtttttt gcctgatgcg acgctggcgc gtcttatcag
    28321 gcctacaggt cccgagcaca gaaccgtagg acggataagg cgttttacgc cgcatccggc
    28381 aaccgttgtc ggaaccgaaa acagcaattc accccgcccg taaaagcatt ttcatttttt
    28441 cgctgcgaca aaactgacgc ttcacttcca ccaccagcgg atggcgtgac agcacaatca
    28501 tcaccacaaa cgccagcacg cccgcggcta tttgcaccgc cagcagcatc cccagcgcca
    28561 gttgcccttt cagcacaatg cccagcgcat aactgaccac cagcgtcggc aacgagagat
    28621 aaaacggcag ccacaaactc aggatgtact ggcgataact ggaaccaaga accggtttaa
    28681 tcatcacgaa gtaactcaga atggtgttga taatctgcac cagcaggaaa ccgagcgtga
    28741 cgccgattgc gcccgccatc tgcccaccaa taacaatcgc cggaataaac agaaatgttt
    28801 tgaatacatt gaatttaaag ctgatatcga cccgcgcttt cgccatcagc agcgaaccaa
    28861 tcgggttacc cacggagcgc agcagaccca caatacacag caattgcagc accggaataa
    28921 tgctgttcca cttttcgccg aataccagcg gtacgaaatt attcgacacc accatcagcc
    28981 ccagcagcgc cggaaagttg ataatcccca ctatcgacag cagcttgtag aagttaacac
    29041 gcagcttttc ggtgtcgtcc tgaattttgg caaacgccgg aaacaacacg cgggtgatga
    29101 ttgggttcag cttcatcggc ggtacgacgg ccacgttgta cgccaggttg tatccccctg
    29161 ccacgcccgc gccaagaata cgcgccagca ccagcgttga aaggttggtg ttgagatagt
    29221 tgatgatgct gtccgccgtc agccaggcac caaaacgtaa gttcggtgcc acagacgcca
    29281 gcgagaagtg cagaccgggg caataaattt tgcgtccaaa gtagccaaac agcagtgttc
    29341 tcaccgcact attgaccaga taaccgagga tcgcggtcat cgccagcggc cagaaatggg
    29401 cgctaactac cgtaaaagtg aagcccgcca gcaccgcgct ggtttcgatc atgccgattt
    29461 tgttgaactc cagctccttc tgcatcaacg cgcggaactg ttgcccgtgg gggatcacca
    29521 caaacgccag cgacaatgtt ttaatcaacg gtgccaggtc cgggttattc agcacgtcgc
    29581 cgatgacatc actcaacaaa aacaccgaca cacataccac aagccccagc ccgacgttca
    29641 gccagtacag cgtggtgagt tcaaggtgac tgatttcttt tcgctgaata atcgagttag
    29701 cgataccgaa gtcagaaagc gtatctgcca gcgcgataat caccagcgac acagtcagca
    29761 ggccgaactg gtggttgtcg ataatccgcg ccagcacggt catctgcacc agcccgaggc
    29821 cgatgatgat caccgtggca atagccgacc acttcgcgcc gctgatggtt ttttcacgta
    29881 agctcatatc aatatgccgc tttgttaacg aagcccttga ataccgtcag gaaaacgatt
    29941 ttgatatcga accagacgct ccattcgcgg atgtactcaa ggtcgaactc gacgcgtttt
    30001 tccattttct ccagcgtgtc ggtttcgccg cgccagccat taatctgcgc ccagccggta
    30061 atgcccggtt tcactttatg gcgcagcatg tagccttcaa tgagctggcg atactgttcg
    30121 ttatgcgcta ctgcgtgcgg acgtggaccg acaatcgaca tccccccggt cagcacattg
    30181 ataaactgcg gcaattcgtc cagcgaggtg cggcgcagaa agttccccac tttagtgacg
    30241 cgcggatcgt tctgcgtcgc ctgggtcacc actttgtcgt tctccatcac tttcatggaa
    30301 cggaacttcc acactttgat cggcttgcca tccataccat agcgagtctg gcggaaaata
    30361 accggccctg gtgaactgag tttcaccgcc agcgcaatac agcacagcac cggggagatc
    30421 agcagcagaa taagcgtcgc cagcacaatg tcttccgcac gttttagcag gcggttaccc
    30481 cccgaaagcg gcgtgtcata cagcggcacc accggaacgc cgttcatctc ttcgagacgt
    30541 gaatggagaa tgttgaaggt aaagacgtcg gggatcagca gcaccgaaca ggtggtatcc
    30601 gccagttgat gggccagttt tttcactcgt gcgccgtcgc acatttgcat cgcgatatag
    30661 acgttatgaa tcttgcccgc ttttgcatcc tcgaccagct gttgcaggtt acccgcccag
    30721 tcgttagaaa cgccgcccgg tttcgtgtcg tggtaaacgc ccaccacttc aaaccctaac
    30781 cacggctgat tacggaaact ctccatcagc atttgtccgg cggctaaatc ccccgctacg
    30841 gcgaccatcc gcttgttgta gccatgatta cgcagccagc ccgccccaat gcgaatacat
    30901 gaacggcaaa ccaccagtcc gatgctggtc agcccatacc acgccagcca gattttcagt
    30961 tgcgtgtcga aatcattgtt gaacgccacc agtccggcgc tgaaaatcac gcttaaggtc
    31021 cagttttgta gcaacagggc aaattctgtc gctgcccgaa caccgcgcca tgagcgataa
    31081 aaatcggtga tgccgcccag catctggaac accaccagcg taatcagcgc caccaacagg
    31141 tgcatgtaga ggaatgacag tccgctgact tcgcaaacca gccatagccc ggcaaacatg
    31201 atggtgatat ctgaaaagcg ttgcaccata gagattaacg atgcattggt tttcgctcgc
    31261 tcgcgctttt ttagatttgt catcgttgtt cctgttatta gccccttacc cgcagtgggg
    31321 taagggaaga tccgacatta ctcgttcagc aacgtcagca gagttcgcgt tcgcgcttcc
    31381 atcagcggca catcaccgcg cgattccaca ttcaggcgca ccaccggttc ggtattggag
    31441 gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcgct gcgatccacc
    31501 gccagcgcct cacggctaaa atgctgttcc acgcggttaa tcgcctcaac gggttgcgcc
    31561 agtttgctgt tgatctcacc gcttgccgga aacgccgcca tccggtcgcg caccagttcg
    31621 cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg gatcatgccg
    31681 ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcgcc accgtagatg
    31741 gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga cattaccggc
    31801 gtgccgcctg cagcagtcac cacatcaata gtgttccagg agagacgcgg atcatggatg
    31861 atcttcgcgc cgggattttt ttcgaggaac gcttctgcca gcaggccgac aatgtagtag
    31921 ccctcgataa actgcccttt ttcgtcaaac agaaaacagc ggtcaaaatc gccatcaaag
    31981 gcaatgccca tatccgcgcc gtgtttgatg accgcattac gggtgtcgtc gcgacattcc
    32041 ggcagcagcg ggttaggaat accgttgggg aaattgccgt ccggtgtgtt gtgtactttg
    32101 attaattcca cgggcgcgcc gagggcttta aagcgggctt caatggcgtc caccaccgga
    32161 cccgctgcgc cattcccgga gttgatcacc agcttgagcg gcgtgagatt tttgacattg
    32221 atataaccga acaggtgatc aacgtaagcg tcacgcaggt tgatttgctg atagcgaccg
    32281 cgtttggttt catcgacggg aggaaagtcg ttggcttcag ccagacgctg gacgtcgcgc
    32341 agtccggtat caccgctgat cgggcgagcc ccctcgcgca ccagcttcat gccgttgtaa
    32401 tccatcggat tatggctggc agtaacttca atgccgccat ccacgccgag atggaacgtg
    32461 gcgaaataga tctcttcggt gccggacata ccgatatcca gcacatcgac gcccgcatcc
    32521 tgtaaacctt ttgccagcgc cagttttaag gtttcgctgg tgagacggac atcaccgcct
    32581 aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg ccaggcgata
    32641 tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa gcaggttaat
    32701 tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac ggccaatttt
    32761 atttttgtcg ttatttacac cgcggtttcg cattcattgc ctgatgcgac gtttacaccc
    32821 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    32881 cttcaattaa atcgagcgga attttccccg ggttttccag gcagtgcgtc gcccccagcg
    32941 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    33001 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    33061 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    33121 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg tgaccatcgg
    33181 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    33241 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg actaggcctg
    33301 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    33361 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    33421 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    33481 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcgagatccg
    33541 gatcgacggc gctcatcgct ttttcgcagg catcgaggat atccgggcga tattttttca
    33601 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    33661 tgcttgctac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    33721 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    33781 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcataag
    33841 gcatggcatt acgcacggcg gcacggaacg cgtctttatc ggcaatcaca tgatccgccg
    33901 ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc agcgccgcca
    33961 gtgcaatggc aggtgcagtg ttacgccctg ccggttcgag aataatgttc tcggtgagtt
    34021 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    34081 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    34141 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataaacggc
    34201 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    34261 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    34321 acgtgccacc gtgttatgtt tgggcagcag gagcgcctga cgaatccccg ccaccagcgc
    34381 ctcgaccgat tccggttcta cgcaaaccgc aatacccgga aaggtttcgc aaagctgccc
    34441 cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca gaatattggt
    34501 cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca gatggcaatc
    34561 gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa attgcatgtt
    34621 gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc cctgcccgac
    34681 aatggcaaaa agcagcggtt catcgcgcag gagatcggca gcttcaataa cgttttccag
    34741 cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat ccggcaggcc
    34801 aagctggtta cgaagggcat caacatcggc gtctgcaaca tgctgaaaac gggcaatttc
    34861 cgaccagttg gggaagaaga tgacgttatc cgccgccacg cctttttcga tggctttatt
    34921 catcatcgaa cgcgaaatcg tggaaacgtt atcgacgtta tgcagtccgc tacgttcgaa
    34981 cgccgttgcc agctgtgcca ctttgccgcc tttgcctttt ccggcaaggc ccagccccag
    35041 catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag agagcttcgc
    35101 cagcaggcgc attcccggcg tgcaaaacag cgttggcact acgccaataa tgcgatccgg
    35161 cttccagcga cgttgcgcca tcagtggaaa gaaactgctg acggcaaaac tgccgagatg
    35221 caacaagcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc gccacaccgt
    35281 ggcggcccct tcttctcgtt tgtagcgcca ggcggaatag ttctcaccca cctgccattg
    35341 cgggtagtaa ggcggtgcgg taatgacccg cacctcatga ccttgtgccg ccaaccattc
    35401 caccatctcg ccggtgtatt tgccgatgcc ggttaactcc ggcgagtagt taatgccgta
    35461 gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa ataggcgcgg
    35521 ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca gcggtaatcg
    35581 tcatgctgct catccggcag taacagctct tcttccgcta ctctgaagcg aaaaccgagc
    35641 accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa atgctgccag
    35701 acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc catcgtcagc
    35761 cgctcaaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg cacaaaccag
    35821 taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcgccgcg actgttctcg
    35881 acaataaagt cgagagagac aagcggtgtg gagcgcacta cagtggcaaa gtcttcctga
    35941 cgtaaaaaca tcattccccc cgaaagcggt cttgattctc aaggaaccac tggtaagtgc
    36001 tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc aggcgcgtca
    36061 catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc acccgacctt
    36121 tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag tcaacgcccg
    36181 tgccgacgtt aatgtgcgac agcatcggtt gggtattctc cagccagact tcatgcgcca
    36241 gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat tcacgcatcg
    36301 gtgtaccgct gccccatacc accacgtccg gcgcattctg tgccgtcgcc tcgtggaagc
    36361 gacgcagcaa tgctggtatc acatgggagt tactcgggtg gaagttgtca tgcggcccgt
    36421 acaggttggt cggcatgaca gagcggtaat cgcgtccata ctggcggttg taagattcgc
    36481 acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc tccagcgtgc
    36541 cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag atgcaggacg
    36601 atccgagaaa catcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg atgttgctct
    36661 caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca acaatgccgc
    36721 ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag aaatcatgca
    36781 cggcgcggct gtccagcagg ttcagttcat cgcgggtacg taataccagc tccacatcac
    36841 cgcgctgttc gagctgccgc ttgatggcag aaccgaccat cccacaatgg ccagcgataa
    36901 aaatgcgttg tttacttatg cttatgactc cagcgcgatc gccacgtcgt agccgtgaga
    36961 tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt cagacaccat
    37021 ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg tcgggtcgcc
    37081 gagcagcgtt tcaacttctg ccggacggaa gtaacgcgga tcaacggcga taatcacatc
    37141 acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc ccttctcttc
    37201 aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gctgccgcca tttccacaaa
    37261 ctgacgtacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct gttcctgttg
    37321 cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac gcagggaatc
    37381 catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga ttgcgcgggt
    37441 gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga agagaattcc
    37501 gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt aggcgtacag
    37561 tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct gcggaatttc
    37621 ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt tcttttccag
    37681 accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga cgtcagcggt
    37741 atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt tgtacacttc
    37801 atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca ggtcgccata
    37861 atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat ccacgcgctc
    37921 ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt tttccagcag
    37981 aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag cgacttttga
    38041 catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg cgtatcacca
    38101 ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca ctgcttcgtg
    38161 cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca tcggttgcca
    38221 gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt tgacttgcat
    38281 ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt gcgccaatgg
    38341 ttatttcacc gagggtatat aaattgacgt catcgccaac ccacgcgtaa tcacctaagg
    38401 ttaatttcca cggataggta atttttactg acggacgaat aactacgttt tttcctattt
    38461 ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt ggcgaccagg
    38521 caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg ccgccccgga
    38581 accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat attcagactt
    38641 tgttatataa ggctttagtc ttacctgtcg tacgttggcg taaatgccag gataattctg
    38701 cccagaagcc aggtacatgt aatatctgcc gttggacttt tttggcgtca gca
//
LOCUS       DADLTF010000019        37643 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.019, whole genome shotgun sequence.
ACCESSION   DADLTF010000019 DADLTF010000000
VERSION     DADLTF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.019"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..174)
                     /locus_tag="JN749_000965"
     CDS             complement(<1..174)
                     /locus_tag="JN749_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HAY7367461.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JN749_000966"
     CDS             complement(258..1142)
                     /gene="yhdJ"
                     /locus_tag="JN749_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HAY7367462.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JN749_000967"
     CDS             complement(1228..1524)
                     /gene="fis"
                     /locus_tag="JN749_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HAY7367463.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JN749_000968"
     CDS             complement(1550..2515)
                     /gene="dusB"
                     /locus_tag="JN749_000968"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAY7367464.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JN749_000969"
     CDS             complement(2844..3725)
                     /gene="prmA"
                     /locus_tag="JN749_000969"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAY7367465.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JN749_000970"
     CDS             complement(3737..5188)
                     /gene="panF"
                     /locus_tag="JN749_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HAY7367466.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5178..5420)
                     /locus_tag="JN749_000971"
     CDS             complement(5178..5420)
                     /locus_tag="JN749_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HAY7367467.1"
                     /translation="MDTSFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JN749_000972"
     CDS             complement(5529..6878)
                     /gene="accC"
                     /locus_tag="JN749_000972"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAY7367468.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHIEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JN749_000973"
                     /gene_synonym="fabE"
     CDS             complement(6889..7359)
                     /gene="accB"
                     /locus_tag="JN749_000973"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAY7367469.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8337..9311)
                     /locus_tag="JN749_000974"
     CDS             complement(8337..9311)
                     /locus_tag="JN749_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7367470.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9463..11403
                     /gene="csrD"
                     /locus_tag="JN749_000975"
     CDS             9463..11403
                     /gene="csrD"
                     /locus_tag="JN749_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HAY7367471.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11708..12751
                     /locus_tag="JN749_000976"
     CDS             11708..12751
                     /locus_tag="JN749_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HAY7367472.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12817..13920
                     /gene="mreC"
                     /locus_tag="JN749_000977"
     CDS             12817..13920
                     /gene="mreC"
                     /locus_tag="JN749_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAY7367473.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13920..14408
                     /gene="mreD"
                     /locus_tag="JN749_000978"
     CDS             13920..14408
                     /gene="mreD"
                     /locus_tag="JN749_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAY7367474.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14417..15010
                     /gene="yhdE"
                     /locus_tag="JN749_000979"
     CDS             14417..15010
                     /gene="yhdE"
                     /locus_tag="JN749_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HAY7367475.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15000..16469
                     /gene="rng"
                     /locus_tag="JN749_000980"
     CDS             15000..16469
                     /gene="rng"
                     /locus_tag="JN749_000980"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HAY7367476.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16537..20337
                     /locus_tag="JN749_000981"
     CDS             16537..20337
                     /locus_tag="JN749_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HAY7367477.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDIRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVDGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20493..21938
                     /gene="tldD"
                     /locus_tag="JN749_000982"
     CDS             20493..21938
                     /gene="tldD"
                     /locus_tag="JN749_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HAY7367478.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFSYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JN749_000983"
     CDS             complement(22066..22995)
                     /gene="aaeR"
                     /locus_tag="JN749_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HAY7367479.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23178..23381
                     /locus_tag="JN749_000984"
     CDS             23178..23381
                     /locus_tag="JN749_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HAY7367480.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23389..24321
                     /gene="aaeA"
                     /locus_tag="JN749_000985"
     CDS             23389..24321
                     /gene="aaeA"
                     /locus_tag="JN749_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HAY7367481.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24327..26294
                     /gene="aaeB"
                     /locus_tag="JN749_000986"
     CDS             24327..26294
                     /gene="aaeB"
                     /locus_tag="JN749_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HAY7367482.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26386..26658
                     /locus_tag="JN749_000987"
     CDS             26386..26658
                     /locus_tag="JN749_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367483.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTLGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JN749_000988"
     CDS             complement(26714..26977)
                     /gene="yhcN"
                     /locus_tag="JN749_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HAY7367484.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JN749_000989"
     CDS             complement(27342..27812)
                     /gene="argR"
                     /locus_tag="JN749_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HAY7367485.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28247..29185
                     /gene="mdh"
                     /locus_tag="JN749_000990"
     CDS             28247..29185
                     /gene="mdh"
                     /locus_tag="JN749_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HAY7367486.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JN749_000991"
     CDS             complement(29247..30314)
                     /gene="degS"
                     /locus_tag="JN749_000991"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HAY7367487.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JN749_000992"
     CDS             complement(30404..31771)
                     /gene="degQ"
                     /locus_tag="JN749_000992"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HAY7367488.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDEKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTNTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31925..32323)
                     /locus_tag="JN749_000993"
     CDS             complement(31925..32323)
                     /locus_tag="JN749_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HAY7367489.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32517..33644
                     /locus_tag="JN749_000994"
     CDS             32517..33644
                     /locus_tag="JN749_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HAY7367490.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33863..34291
                     /gene="rplM"
                     /locus_tag="JN749_000995"
     CDS             33863..34291
                     /gene="rplM"
                     /locus_tag="JN749_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAY7367491.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34307..34699
                     /gene="rpsI"
                     /locus_tag="JN749_000996"
     CDS             34307..34699
                     /gene="rpsI"
                     /locus_tag="JN749_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAY7367492.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35094..35732
                     /gene="sspA"
                     /locus_tag="JN749_000997"
     CDS             35094..35732
                     /gene="sspA"
                     /locus_tag="JN749_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HAY7367493.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35738..36235
                     /gene="sspB"
                     /locus_tag="JN749_000998"
     CDS             35738..36235
                     /gene="sspB"
                     /locus_tag="JN749_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HAY7367494.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36278..37606)
                     /gene="dcuC"
                     /locus_tag="JN749_000999"
     CDS             complement(36278..37606)
                     /gene="dcuC"
                     /locus_tag="JN749_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7367495.1"
                     /translation="MGYLILKNYKPQVVLAAAGVFLMMCGVWLGFGGVLDPAKSSGYL
                     IVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSLLSRPLKLIRSPYI
                     ILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGI
                     LETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQA
                     EQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVVMLLSLTVTMFVEF
                     FRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSG
                     LGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATT
                     LARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         9115 a         9414 c         9904 g         9210 t
ORIGIN      
        1 tctgtcctga aaattcagtt cgtcacagtt aaccagtttt agtggattaa ctgagttcat
       61 attacggcat ttgtctgttt ctgttgtcag cagttcaatc cactgcatca gtaaagtatc
      121 aaacagaaaa acgaagacag cgaaaacgac gagccaccaa tacttgcgaa tcattacctg
      181 agcctgtgca tgtttaacca agtctggcag gaaatataca cgaaatgaat aatctgaaaa
      241 ccaggcttac acttccttta ctttgcaatg agatcggggt caacttcgct taaccggctt
      301 cgttttgaca ggttgccggt ctttcttttt ttcactttcg ccagttcttc cgcggagtaa
      361 tgcgacgcga catccagccg tcgaagcccc attttgatgt actcgctgtt gatctcaata
      421 ccaatgaatt ttcgtccgct ggcgatggct acggcaccgg tagtaaagct accggcaaac
      481 gggtcgagaa cgatatcgcc tgggttggaa gaggcgagaa taatgcgttt cagtaaggct
      541 ttcggttttt gcgtcgggtg gttctcatat tcatccatca aataacgcac gcgtggaaaa
      601 tcccagacgt tacccggtac tttttgatga ttgtatggct gtggaggatt tttgcgataa
      661 tagatcaacg cgcgctgcga tccggttttg gcttctacca gaatagcatc accgttgaat
      721 gtgtagttct ttgcgtcttt caccatcatc aggatgggtt cgtacatgga gccgtagtgt
      781 tttttcgcct gtactccaga actgtcatat gaccagacga tgcgactttt gatggtaaaa
      841 agcttgcggc actggagatc gataaagggc atgttttccg tactgttcat aatgtacatg
      901 ctgccctgct ttttcagaac gcggtggcac tctgcaatca cttcaaacag ccagtcgata
      961 aacagatctt ctttccaggc ttcgatcaaa ccatcaaaat ttttaccgat gttatacggt
     1021 gggtcggcaa agagcagatc gacgctttca gtaggtagct ttttaagttc ggcaagggca
     1081 tcaccgtgaa taatggtctt agcttcatta ccaaaccggg tcggttcaca tcctgttctc
     1141 atggtcactc cctttgtgac acctataaaa aaggcgcttc cctatgccga gtagcgcctt
     1201 tttaatcaag catttagcta acctgaatta gttcatgccg tattttttca attttttacg
     1261 cagcgtacca cggttgatgc ccatcatcag cgcagcacgg gtctggttac cacgggtgta
     1321 ttgcatcacc atgtccaaca ggggctgttc tacttcagcc agtaccagct catagaggtc
     1381 attcacatcc tgaccattca gttgagcaaa atagttcttc agtgcctgtt taaccgagtc
     1441 acgcaggggt ttttgggtta cctgatcctg agagttaacg gtagaaacgg tcagtacgtc
     1501 agaatttacg cgttgttcga acatagttct gtcagctctt tatttctgtt tacgcaaaat
     1561 tttcgaagta tgcctccaac gcctccagct gttcgctggc atcctcaatg gcgttgaatg
     1621 tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata ccaggaaacg tgtttacgtg
     1681 caattcggta cccttttgcc ggaccataaa agtcatgcag ttcccgaacg tgcgcgcaaa
     1741 gcaagcgctt aacctctgcc aaaggcagcg ggggcagcaa ctccccagtg tccagataat
     1801 gctggatttc ccgaaagatc cagggtcttc cctgagctgc gcggcctatc atcagggcat
     1861 ccgcccctgt atagtcgagc acagctctgg ctttaagcgg gtcagtaatg tcgccattcg
     1921 cgataaccgg aatggaaact ttctgcttaa ctgcccgaat actgtcgtac tcagcttctc
     1981 cattgaacaa acaggcgcgt gtacggccat gaatggtcag agcctgaatg ccacagtctt
     2041 cagccagttg ggcaatctct tcgcagttac ggtgttccgg tgcccagccg gtgcgaatct
     2101 tcagggtaac aggaacgtcc actgcattga cgacctcggt aaggatcgat ttaacgacat
     2161 ccgggtactg caagagggct gaacctgcga gcttgcgatt cactttttta gccgggcaac
     2221 ccatattgat atcaataatc tgggcaccgc tttccacgtt aatacgtgct gcatctgcca
     2281 tttctttcgg agcgctacca gcaatttgca cggtgcgaat accgggttca tcaatgtgca
     2341 ccatccgtaa acgagatttg tcgctttccc aaacctgtgg gttagaagac atcatctcgg
     2401 atactgtcaa tccggctccc atctcgtagc acaacgtccg aaaaggtctg tctgtaatgc
     2461 cagccatggg cgctgcgatc aggcgatttc tgagctgata ttgtccgatg cgcatgagtt
     2521 aagaaatgac catactgtga ctgcaaggcg gcgtatatta cgcatttttt gcacgagatg
     2581 aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag aattgcattt aaaatgagcg
     2641 tggtgcgata attactcata aaaatcatca tattagaaaa tagtgactaa aatttacact
     2701 caaagaaatt tgagtaagtt ctcaattttt ctttatgaat gaaaattttg gcacgcaaat
     2761 ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc ttgctcgcac ttcgccccgc
     2821 gtcaccctac ggcgatgcga aggttaattc ttacgaccgg taatacggca ccactcttct
     2881 ttttccacga ccgggtccag tgcgaagcta tcggcataag cttcacaaac gctctctgcc
     2941 tggcttgcca gaataccgga aaggcccagc aaaccgcctg aaaccggcag gacgctgatt
     3001 aacggtgcca gttcacgtaa tgggcctgca aggatgttag cgaccaccac gtcggctttc
     3061 atttcttctg gctgatcttt cggtaagtag agttccagac ggtcagaaac gccattacgt
     3121 tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga tatcaatacc aatggctttt
     3181 gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg aaccacagcc aaagtcgatg
     3241 actgttttac cggttaaatc gaggctgtcg agccattgca ggcacagaga ggtggttgga
     3301 tgggtacccg taccaaacgc cagccctgga tctaacatca cgttgacggc gttttcgtcc
     3361 ggcacatcac gccagctagg gcagatccac agtcgttcac caaagcgcat cgggtggaaa
     3421 ttatccatcc attcgcgctc ccagtcttta tcttctagtt gttcggtttt atgcgcgaag
     3481 cctgcgccga gcagcggatg gttttccaga atcgccacca cgtcgttcat atcggtttca
     3541 gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc gcgtttcgcc cggcagcggc
     3601 tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag aaacggcacc cgcttccatc
     3661 agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt tcagtttcag ttggatccaa
     3721 ggcatggcaa aactctttat ttatcagtag tcaaaacggt agcttgcggg acggatgtac
     3781 cgaaacggtt tccgaccagg aaagccagca aacttagtag taacgagggc acgatagggt
     3841 ggaagcccag gtactgaata ttcagcgtcg cgagtacggc atacagcacg ccgccaacga
     3901 tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca gtaaagaccc agcaccagcg
     3961 gccacaggaa aacggcttcc agcccaccga aggccagcaa attcagccag atgatcattt
     4021 ctggcggctt ccaggcggca agcagcagca acgcgccgag aactaacgta attaccgccg
     4081 acatccgctt cagacgcgtc tcgttttgca tttgatccgg acggatattc agatagagat
     4141 ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt aattgtcgac atgatcgcag
     4201 ccataggtgc agccagaaag atcccggcag caaaaggtgg cagcactttt accattaacg
     4261 tcgggatcac cagatccggt acggtgagat cggggatcac tgcacgacct aacgctccgg
     4321 ctaggtgcat accgaacatc aggattgcga ctacaatcgt accgatgatg atcccccggt
     4381 gtactgcttt gctgtcttta taagagatac agcgcaccgc agtatgcggc aggccaatca
     4441 cgccaaagca caccagtacc cagaacgacg tcataaaggc aggcgacaga atatcgtcag
     4501 cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa ggtctgtact gcgttgctta
     4561 agccgccagc ggcatgtacc acgccaataa gcagcacaac ggtgccaatc agcatcacaa
     4621 gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc accaaaggcg gtatataacg
     4681 caatgctgat accaaaaatc agcagcccgg tttcataagg aatacccgcc gcggtttcca
     4741 gcaggcgcgc accgccgata aactgcacgg tcatcgcacc aacgaacgca accagcaaac
     4801 tcaaactcgc cagccacacc agaagacgac tctggtagcg ggcaaacagc atatcgttca
     4861 gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt cttgccgaga ataccgagtg
     4921 aaagccagac agcaggaagc tgaatcatcg ccagcaatac ccagcccagc ccgtatttat
     4981 aagcagctcc tggcccgccg ataaacgaac tggcactgat ataggtcgcg gtgagcgtca
     5041 tcgccagcac aataccgccc atagagcggc tgccgaggaa atactcatta aggaaggtgc
     5101 cggtgctccg tttacgcatc gcataaaccg agataccgaa caccaccacc agataggcga
     5161 ccagcggtag aattacttca agctgcatcg tcatcctcca gtgggatatc gcgatagata
     5221 aatttcacca tcgcccagca cagtccaata aacagtagcg gcgtcaggat gcaggccatc
     5281 tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc caggtaagta agcatctact
     5341 aaccaaactg ccagatacaa aagggtcagc cccagcgccc agcgcgcctc tttatgggcc
     5401 tgaacaaaac tagtgtccat tttttgtccc tgatgggtga agaaagcgag gattgtacct
     5461 tatgggggtt gtcgatcccc agtaataaaa aaggccggaa aatccggcct tttgacgctt
     5521 ttgcagtctt atttttcctg aagaccgagt tttttctcca gatagtggat gttagtgcca
     5581 ccatgctgga agttctcgtc attcatgatg cggatctgca gatcaacgtt ggttttgata
     5641 ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg caatcgccac gtcacggttt
     5701 tcaccgtagc aaatcagctt accgatcatt gagtcatagt acggcggtac ggtgtagccc
     5761 gcgtagatat gagactccca acgtacgcca aaaccgccag gtgcgtggaa acgggtgatt
     5821 ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt tgatacgaca ttccaccgca
     5881 tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg gttgaccggc agcgatacgc
     5941 agctgttctt tgatcaggtc aacgccggtg atcatttctg taaccgggtg ttctacctga
     6001 atacgggtgt tcatttcgat gaaatagaac tcgccgtttt cgaacaggaa ctcgaaagta
     6061 cctgcaccgc gatagccgat atcaacacac gctttagcgc aacgttcgcc gatgtagcga
     6121 cgcagttccg gggtaatgcc cggtgctggt gcttcttcga ccactttctg gtggcggcgc
     6181 tgcatggagc agtcacgttc cgccagatag atagcgttgc cctgaccgtc agccagtacc
     6241 tgaatctcga tgtggcgagg attctccagg tatttctcca tgtaaaccat atcgttgctg
     6301 aaagcagctt tcgcttccgc acgggtcatg gagatggatt gtgccagttc agcgtcgccg
     6361 cgcactacgc gcataccgcg accgccgccg ccgccggagg ctttgataat caccggataa
     6421 ccaatgcgtt tagcaatggc gcggttttta tccatatcgt cgcccagcgg gccgtcagaa
     6481 ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga ttgcggatac tttgtcgccc
     6541 atcaggcgaa tggtttctgc tttcgggccg atgaagataa agccggagcg ttcaacctgc
     6601 tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg gatggattgc tactgcgccg
     6661 gtgatttcag cggcgctgat gattgccggg atgttcagat aactttgtac tgacggagca
     6721 gggccaatac agaccgtttc atctgccagt aatacgtgtt ttagatcgcg atccgcgctg
     6781 gagtgcacag cgacagtctt gatgcccagt tctttacagg cacgaagaat acgcaatgca
     6841 atctcgccgc ggttggcaat aacaatttta tccagcatgt tcgcctcgtt actcgatgac
     6901 gaccagcggc tcgtcaaatt ctaccggttg tccactttcg accagaattg ctttcacggt
     6961 accggatttg tccgcttcga tctggttcat cattttcatg gcttcaacga tgcacagggt
     7021 atcgcccacg ttgactttct gacccacttc gatgaacgct tttgcgtccg ggcttggggt
     7081 gcggtagaaa gtaccaacca tcggggaacg tacgatgtga ccactgattt ccgctgctgc
     7141 tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg ttagattgag ctggctgctg
     7201 catcattggt gcagcgtaag cttgttgcat cacagggaaa cttgcggcag gagctgcacg
     7261 gctaatgcgt actgactctt cgccttcaga aatttccagt tcggagatgc ctgattcttc
     7321 aaccagctcg atcagttttt taatcttacg aatatccatg agtgggttcc gtactctttg
     7381 tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa agcgtatgcc ccgacagctg
     7441 tatgcatagc gataaattcc agcaggccgg gacgccggtc tattttgccc cagagtgtca
     7501 atgttagact tgacggacat tgtgcagcga tctgctgcta tcctccggca aaaaacaaaa
     7561 tataccttcg ttgcgccaat gtcacctttt ccgcagcgca aaaactgcgt cagggaggac
     7621 gaggtcgcac attataacga tttcgtagca attggcagct aaatactggt cttatcaggg
     7681 aagataatca acagctaaca tgtaaataac cttcaacacc gtgtaatttg caacaagccg
     7741 cacaattcac gaaattagcg ccatcatcga cggaacttct tataacgtaa ggtgaaaagc
     7801 tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga taatcttcac agaccacaaa
     7861 taatgtaggg atgccaggat cgcgacaaga tagacgacat tacgcagcgg ttgctgggga
     7921 ttacccaatt ttcactgcat cgtctggata gatgtgaatg ctaaagcacg caaaatcaac
     7981 caactgacaa ttctcagcat taaataggtt cgagtggtta atttgtagtc aaataaaatc
     8041 agattattca ccccatggta ccagaaatca gtaacaggtt aagcgtagtg tcgcccaggc
     8101 aaagcaccat aaacctaaca ggcgacggag gcgtatcaat aatggctgtt tagaggagta
     8161 tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt tccagtttgg atgatgttgt
     8221 acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc taaccgcttt tcccgttaac
     8281 cgattgaata atgatgtcaa aatgagttcc ctctcttatt attccttgct aaatggttag
     8341 ttaaccttca ccagcgtgcg accttggatc tggttattaa tgatggcctc ggcaaagttc
     8401 ggtgcctctg acagagatat ctcttttgcc gcctgggtat agaatgattc cggtaaatcg
     8461 gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg gcgtcattac tgaatccacc
     8521 ccttgcaaac ggacattacg cagaataaat ggcatgaccg tgcttggcag agtaaaacca
     8581 cccgccagac cacaggccgc cacgcagccg ccgtaattca tttgcgccag cacttttgcc
     8641 aggactttgt cgccaacagt gtcaattgcc ccagcccaga cttgtttttc cagaggacgg
     8701 gattcggcaa actcatcacg agggagaata cggctggcac ctaaactttt cagatattca
     8761 tgggtacttt cgcgaccgga aacggcaacg acctgataac ccaacttatg cagcagcgcc
     8821 acggcggtac tgccgacgcc accactggca cccgtcacga caatctcccc gtcctgcggg
     8881 cgaacaccgg catcttccag cgccatcaca cacagcatgg cggtaaaacc ggcagtaccg
     8941 ataatcattg ctttacgcgc gtccagccct tgcggcatgg caaccagcca gtcacctttc
     9001 actcgcgcct gctccgccag cccaccccag tggttttcac caacgcccca gccagtgagt
     9061 aacacctcct gaccggcatg aaaacgcgga tcttcgctgg tgcgtacagt tccggcaaaa
     9121 tcgatcccag gaatcatcgg aaaattacgg atgatttttc ccttaccggt aatcgccagc
     9181 gcatctttat agttcaggct cgaccagtga acatcgaccg tgacatcgcc ctccggcagg
     9241 cgactttcgt ccagagtctg tactgatgcg agagttttgc cgtcctgctg ttctaaaagt
     9301 aacgcctgca taagtggtcc tcatgtgcat gatggattgg aaaataattt ctgaagacta
     9361 tactcgctaa tggaaaagca aagccgatga agcgcaataa attgcgagat aaatctgatt
     9421 tgctagtatg cccgcttcct cactatcgga gttaacacaa ggatgagatt aacgacgaaa
     9481 ttttcggcct ttgttacgct gctcaccggg ttaacaattt ttgtgacttt gctgggctgt
     9541 tcgctaagtt tctacaacgc cattcagtat aagtttagtc atcgcgttca ggcggtggcg
     9601 acggcgattg atacccacct tgtgtcgaat gacttcagcg cattaaggcc acaaattacc
     9661 gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc tgcatggtga taaacaggtt
     9721 tataccctgg ccagaaatgg tagttatcgt ccggttggca ccagcgatct gtttcgtgaa
     9781 ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc gtctggttta tcaggatccg
     9841 atgggcaact atttccattc gttgatgacc accgcgccgc tcacgggggc gattggcttt
     9901 atcattctta tgctctttct ggcggtacgc tggttacaac ggcaacttgc cgggcaagaa
     9961 ttgctggaaa cccgggctac tcgtatctta aacggtgagc gtggctctaa tgtgttggga
    10021 accatctatg aatggccgcc cagaaccagc agtgcgctgg atacgctgct tcgtgaaatt
    10081 cagaacgcac gcgaacaaca cagccgtctt gatacgctga tccgctctta tgccgcacag
    10141 gacgtgaaaa ccggcctcaa taaccgactc tttttcgata atcagttagc aacgttactg
    10201 gaagatcagg agaaagtagg tacccacggg atcgtgatga tgattcgtct gccggatttc
    10261 aatatgttga gcgatacctg ggggcacagc caggttgaag aacagttctt cactctgacg
    10321 aatctgctgt cgacatttat gatgcgctac cctggcgcac tgctggcgcg ttaccaccgc
    10381 agtgattttg ctgcgctgtt accgcaccgg acgttaaaag aggcagagag catagccagt
    10441 cagttaatca aagccgtcga taccttgccg aacaataaaa tgctcgatcg cgacgatatg
    10501 atccacattg gtatctgtgc ctggcgtagt ggtcaggata ccgagcaggt aatggaacat
    10561 gcagagtctg ccacgcgtaa tgcgggattg cagggtggca atagctgggc tatttatgat
    10621 gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc gtacgcttat cgagcaaatg
    10681 ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg ttactcgcga aggtcaggtt
    10741 catcatcgcg aactcatgtg ccgcatcttc gatggtaatg aagaggttag ctcggcggag
    10801 tatatgccga tggtcttgca gtttggctta tcggaagagt atgaccgtct gcaaatcagc
    10861 cgtcttattc cactattgcg ttactggcca gaggaaaatc tggcgattca ggttaccgtt
    10921 gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg atacgttaat gcaatgtgaa
    10981 aaatcacaac gaaaacgcat aattattgaa cttgcagagg ccgatgtagg tcaacatatc
    11041 agtcgtttac aacctgttat tcgtttagtg aatgctttag gggtacgggt agccgtcaac
    11101 caggctggtt tgacgctggt aagcaccagt tggatcaaag aacttaatgt tgagttactc
    11161 aagctccatc cggggctggt caggaacatt gagaagcgaa cggagaacca gctgctggtt
    11221 caaagcctgg tggaagcctg ctccgggacc agcacccacg tttacgccac cggcgtgcgt
    11281 tcgcgaagcg agtggcagac cctgattcag cgcggtgtta caggcgggca aggggatttt
    11341 ttcgcgtcct cacagccact tgatactaac gtgaaaaaat attcacaaag atactcggtt
    11401 taacctgccg tttaatccgt tttcacgtag aataacgcgc gctgcgtctc atgggagtgt
    11461 gcttgtctgc tcgccagatt gttgcagcac atatgcagat gaatgacctt acgcggttgc
    11521 aaacaggcga ggaatgctgc tgatgcatta agcctttctg gactcaggca gagatttgta
    11581 acaaaggaaa cgaactgcac taattttcac cgtagcagat gatttttgcg ccttgtcgct
    11641 gctgcgtgtg gttggtaaag taagcggatt ttcttttccg ccccagcttt caggattatc
    11701 ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat gacttgtcca ttgacctggg
    11761 tactgcgaat accctcattt atgtaaaagg acaaggcatc gtattgaatg agccttccgt
    11821 ggtggccatt cgtcaggatc gtgccggttc accgaaaagc gtagctgcag taggtcatga
    11881 cgcgaagcag atgctgggcc gtacgccggg caatattgct gccattcgcc caatgaaaga
    11941 cggcgttatc gccgacttct tcgtgactga aaaaatgctc cagcacttca tcaaacaagt
    12001 gcacagcaac agctttatgc gtccaagccc gcgcgttctg gtttgtgtac cggttggcgc
    12061 gacccaggtt gaacgccgcg caattcgtga atccgcgcag ggcgctggtg cccgtgaagt
    12121 cttcctgatt gaagaaccga tggctgccgc aattggtgct ggcctgccgg tttctgaagc
    12181 gaccggttct atggtggttg atatcggtgg tggtaccact gaagttgctg ttatctcctt
    12241 gaacggcgtg gtttactcct cttccgtgcg cattggtggt gatcgtttcg acgaagctat
    12301 catcaactat gtgcgtcgta attacggttc tctgatcggt gaagccaccg cagaacgtat
    12361 caagcacgaa atcggttcgg cttacccggg cgatgaagtc cgtgaaatcg aagttcgtgg
    12421 ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg aactccaacg aaatcctcga
    12481 agcactgcag gaaccgctga ccggtattgt gagcgcggta atggttgcac tggaacagtg
    12541 cccgccggaa ctggcttccg acatctccga gcgcggcatg gtgctcaccg gtggtggcgc
    12601 actgctgcgt aaccttgacc gtttgttaat ggaagaaacc ggcattccag tcgttgttgc
    12661 tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa gcgctggaaa tgatcgacat
    12721 gcacggcggc gacctgttca gcgaagagta atcggatgca ggcaggggaa gcgtctgttt
    12781 accctgcctg gtctgatacg agaatacgca taacttatga agccaatttt tagccgtggc
    12841 ccgtcgctac agattcgcct tattctggcg gtgctggtgg cgctcggcat tattattgcc
    12901 gacagccgcc tggggacgtt cagtcaaatc cgtacttata tggataccgc cgtcagtcct
    12961 ttctactttg tttccaatgc tcctcgtgaa ttgctggatg gcatatcgca gacgctggcc
    13021 tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc aggaactgtt gctgaaaaac
    13081 agtgaactgc tgatgcttgg acaatacaaa caggagaacg cgcgtctgcg cgagctgctg
    13141 ggttccccgc tgcgtcagga tgagcagaaa atggtgactc aggttatctc cacggttaac
    13201 gatccttata gcgatcaagt tgttatcgat aaaggtagcg ttaatggcgt ttatgaaggc
    13261 cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg tggccgtcgc taaactgacc
    13321 agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc caatccaggt gctgcgcaac
    13381 gatatccgcg taattgcagc cggtaacggt tgtacggatg atttgcagct tgagcatctg
    13441 ccagcgaata cggatattcg tgttggtgat gtgctggtga cctccggtct gggcggtcgt
    13501 ttcccggaag gctatccggt cgcggttgtc tcttccgtaa aactcgatac ccagcgcgct
    13561 tatactgtga tccaggcgcg tccgactgca ggactgcaac gtttgcgtta tctgctgctg
    13621 ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc cggaagaggt gcatcgtgtt
    13681 gctaatgaac gtctgatgca gatgatgccg caggtattgc cttcgccaga cgcgatgggg
    13741 ccaaagttac ctgaaccggc aacggggatc gctcagccga ctccgcagca accggcgaca
    13801 ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg ctgctaatcg ctctccacaa
    13861 agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc gtgcgccggg agggcaatag
    13921 tggcgagcta tcgtagccag ggacgctggg taatctggct ctctttcctc attgcgctgt
    13981 tgctgcaaat catgccctgg ccggataacc tgattgtttt ccggccaaac tgggtgttac
    14041 ttatcttgtt gtattggatc ctggccttgc cccatcgcgt aaatgtgggc acaggttttg
    14101 tgatgggtgc catactggat ctgatcagcg gctcgatgct tggcgtacgc gtattggcga
    14161 tgagcatcat tgcttacctg gtggcgctga aataccagct tttccgcaac ctcgcattat
    14221 ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt ggatattatt gttttctggg
    14281 cagagttttt agtgattaac gtctctttca gaccggaagt gttctggagt agtgtagtca
    14341 atggggtgct ctggccgtgg attttcttgc tgatgcgtaa agtccgtcag cagtttgccg
    14401 tgcaataaag gtttctatga cttctctgta tttagcttcc ggttctccgc gtcgtcagga
    14461 gttacttgcg caacttggcg tgacctttga acgtattgtt acgggcattg aggagcagcg
    14521 tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg gcacgcgaga aagcacaggc
    14581 gggtgtcgcg caaacggcac aggatctccc ggtgctgggt gcggatatta tcgttatcct
    14641 gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat gcggcgcaga tgttgcgcaa
    14701 attatcgggt cagacccatc aggtgatgac ggcagtggcg ttggccgaca gccagcatat
    14761 tctcgattgc ctggtggtca ccgatgtgac tttcagaacg ttaacagacg aagacatcgc
    14821 gggctatgtc gccagcggtg aaccgttaga taaagcaggt gcatacggta ttcaggggct
    14881 gggtggctgt tttgtcagga agataaatgg cagctatcac gccgtagtcg gcttaccgct
    14941 ggttgaaacg tatgaattat taagtaattt taacgcactg cgtgagaaaa gggataaaca
    15001 tgacggctga attgttagta aacgtaacgc cttcggaaac gcgagtggcg tatattgatg
    15061 gcggtattct gcaggaaatt catattgaac gtgaggcgcg acgcggaata gtaggcaata
    15121 tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca ggcggctttt gtagatattg
    15181 ggctggataa agccgcgttt cttcatgcat ccgacatcat gccgcatacc gaatgtgtgg
    15241 cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc ggaactggtt cgtcaggggc
    15301 aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac taaaggtgcg cgcctgacca
    15361 ccgatatcac gctgccttct cgctatctgg tatttatgcc aggggcttct cacgttgggg
    15421 tttcccaacg aattgaaagc gaatcagaac gtgaacgcct gaaaaaagtg gtcgcagagt
    15481 attgcgacga gcagggcggg tttatcatcc gcactgcagc ggaaggggtt ggcgaggctg
    15541 aactggcctc cgatgccgct tatctgaaac gcgtctggac caaagttatg gagcgtaaaa
    15601 agcgcccgca gacccgttat cagctgtacg gcgaactggc gctggcgcag cgtgttctgc
    15661 gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga ctcacgcctg acttacgaag
    15721 cattgctgga gtttacctcg gagtacattc ccgagatgac cagcaagctg gagcattaca
    15781 ccggacgcca gccgattttc gatctctttg atgtcgaaaa cgaaatccag cgagcgttgg
    15841 aacgcaaagt agaactgaaa tccggtggtt atctgattat cgaccagacc gaagcgatga
    15901 ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg caatctggac gacactattt
    15961 tcaataccaa tattgaagcg acgcaggcta tcgctcgcca gttacggttg cgtaatctgg
    16021 gcgggattat cattattgat ttcatcgata tgaataatga agatcaccgc cgccgcgtgc
    16081 tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa aaccagcgtt aatggttttt
    16141 cggcgctggg gctggtggag atgacgcgta aacgcacccg cgaaagcatt gagcacgtac
    16201 tgtgtaacga atgcccaacc tgccacggtc gcggaacggt gaaaaccgtg gaaacggtat
    16261 gctatgaaat catgcgcgag attgttcgtg tccaccatgc ttacgactcc gaccgtttcc
    16321 tggtctatgc ttctccggca gtagccgaag ccttgaaagg cgaagagtca cactcgctgg
    16381 cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca aattgaaccg ctctataacc
    16441 aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg gccgccatcc ggcaaagggt
    16501 ttttgagtca catttttagc agacaaggag tgacgggtga ggcgattgcc ggggatttta
    16561 ctgcttactg gagccgcgct cgttgtgatc gctgccctgc tggttagcgg cctgcgtatt
    16621 gctttaccgc atcttgacgc ctggcgtccg gaaatcctca acaaaataga atccgcgact
    16681 ggcatgccgg tagaagccag tcagctctca gccagctggc agaattttgg cccgacgctt
    16741 gaagcacacg atatccgtgc agaactaaaa gatggcggcg aattttcggt taaacgcgtt
    16801 actctggcgc tggatgtctg gcagagcctg ttacatatgc gctggcagtt tcgcgacctc
    16861 actttctggc agctgcgctt tcgcaccaac actcctatca ccagcggtgg tagcgatgac
    16921 agtctggaag ccagtcacat cagcgatctg tttcttcgtc aatttgacca tttcgatatc
    16981 cgcgacagtg aagtcagttt tctgacgcca tccggtcaga gcgccgagct ggcgatccca
    17041 caactcacct ggctgaacga tccacgtcga caccgtgcgg aaggcctggt aagcctctcc
    17101 agccttaccg ggcagcacgg cgtgatgcag gtgcgcatgg atttgcgcga tgatgagggg
    17161 ttgttaagca atggtcgcgt ctggctacag gcggatgaca tcgacctgaa gccgtggctc
    17221 ggtaaatgga tgcaggacaa tattgctctg gaaacggcac agttctccct tgaaggctgg
    17281 atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct ggctgaaaca gggcggtgcc
    17341 agctggttgg gcgagaagca aacgcatacg ctgtcggtgg ataatctgac cgcgcatatt
    17401 acgcgtgaaa atccgggctg gcagttctct attcccgata cacggatcac gatggacggc
    17461 aaaccctggc cgagcggagc attgacgctg gcctggatac cggaacagga cgttgacggc
    17521 aaagacaata aacgcagtga cgaactccgg attcgcgcta gtaatctgga gctggcaggc
    17581 ctggagggca tacgcccgct ggccgcgaaa ctttcacctg cactgggtga tgtttggcgc
    17641 tccacacaac cgagcggcaa gattaacact ctggcgctgg atatcccgct tcaggcggca
    17701 gacaagaccc gttttcaggc atcgtggagc gatctggcct ggaagcaatg gaaattatta
    17761 ccgggtgcgg aacacttctc cgggacgctt tccggcagcg ttgaaaatgg tttgcttacc
    17821 gcgtcgatga agcaggcaaa gatgccttac gaaacggtat tccgtgcgcc actggaaatc
    17881 gccgacggcc aggcaactat aagctggctg aacaatgaca aaggtttcca gctggatggg
    17941 cgtaatattg acgttaaagc caaagccgtc catgcgcgcg gcggttttcg ttacctgcaa
    18001 cctgctaacg atgaaccctg gctgggtatt ctggctggca tcagtaccga tgatggttca
    18061 caagcctggc gctatttccc ggaaaacttg atgggtaaag acctggttga ttatttaagt
    18121 ggtgcgattc agggcggtga agcggataac gcgacgctgg tttatggtgg caatccgcaa
    18181 ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc tggtgccgct gcgcaacgcg
    18241 aagtttgcct tccagccgga ctggcctgca ttaactaacc ttgatattga actggacttt
    18301 attaacgacg gtttatggat gaaaaccgat ggcgttaatc tgggcggcgt gcgcgcgagt
    18361 aatctcaccg cagtgatccc tgactactca aaagaaaaac tgctgattga cgctgacatt
    18421 aaaggtccgg gtaaagccgt tggcccttac tttgatgaga caccgctgaa agattctctg
    18481 ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga atgctcgctt acatcttgat
    18541 atcccgctga acggcgaact ggtaaccgcg aaaggtgaag tgacgctgcg taataacagt
    18601 ctgtttatca aaccactcga cagcatcctg aaaaatttga gcggtaaatt cagctttatc
    18661 aatggcgatc tgcaaagtga accactgaca gcaagctggt ttaatcagcc gttgaacgtg
    18721 gatttttcca ccaaagaagg ggcaaaagcc taccaggttg cggtgaatct caacggtaac
    18781 tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga acgaagcatt gagtggcagc
    18841 gtggcgtggg atggtaaagt gggcattgat ctgccttatc atgctggcgc gacctataac
    18901 gtagagctga acggcgatct gaataatgtg agtagtcact taccttcacc gttagccaaa
    18961 cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg gcaatctcaa cagctttgaa
    19021 ttaaccggac aggctggtgc ggataaccat ttcaatagcc gctggttgct tggtcaaaag
    19081 ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa cgctcccgcc gttgccggaa
    19141 caaagtggcg ttgaacttaa tatgccgccg atgaatggtg ccgagtggct ggccctgttc
    19201 cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt tcccacaaca cataacatta
    19261 cgtacgccta tgttgtcact gggaaatcag caatggaata acctgagtat tgtttcgcaa
    19321 ccgacggcaa atggcaccct ggttgaggcg caagggcgtg aaatcaacgc cacgctagcg
    19381 atgcgtaata acgcgccgtg gctggcgaat atcaaatatc tttattacaa cccgagcgtg
    19441 gcgaaagctc gtggtgattc aacgccgtca tcacctttcc cgacaacgga gcgcattaac
    19501 ttccgtggct ggccggacgc acaaatacga tgcacagagt gctggttctg ggggcaaaaa
    19561 ttcggtcgca ttgacagtga tatcaccatt tctggcgata cgttaacgct gaccaatgga
    19621 ctgattgata ctggtttctc gcggcttact gccgatggtg aatgggttaa taatccgggg
    19681 aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga aaattgatgc cgccgcagaa
    19741 ttttttggtg tcacgacgcc catacgccag tcgtcattta atgtggatta cgatttacac
    19801 tggcgcaaag caccctggca gccagatgaa gcgacgttga atggcatcat tcatactcaa
    19861 ctgggtaaag gcgaaattac cgaaatcaat accggacatg ccgggcaatt gctgcgctta
    19921 ttgagcgtag atgccctgat gcgtaagctg cgttttgatt tcagagacac ttttggcgaa
    19981 gggttctatt ttgactccat tcgcagcacc gcgtggatta aagacggcgt tatgcacacc
    20041 gacgacacgc tggtggatgg cctggaggcg gatatcgcca tgaaagggtc ggtaaatctg
    20101 gtacgtcgag acctgaatat ggaagcggtt gtcgcaccag agatttctgc gacggtgggc
    20161 gtggctgcgg cttttgcggt taaccccatt gttggcgcgg cagtgtttgc cgccagtaaa
    20221 gtgctggggc cgctgtggag caaagtctcc attttgcgct atcacatttc gggtccgctg
    20281 gatgatccgc aaatcaacga agtgttgcgc caaccgcgta aagaaaaagc gcaatgattt
    20341 gacgagggcg cgtaattgcc ccaatctcat atgataatcg ttgccaaagg ccaacgagcc
    20401 ggaacataac tgtaggccgg atagggcgtt cacgccgcat ccggcagccg taaaaaatcc
    20461 tctactgcag taactaacga gtagcaaaaa cgatgagtct gaacctggta agtgaacaat
    20521 tgctagcggc gaacggcctg aaacatcagg acttgttcgc gatcctcggt caactggccg
    20581 aacgtcgcct tgattatggc gatctctatt ttcagtcgag ctatcacgaa tcctgggttt
    20641 tagaagaccg cattattaaa gatggttctt acaacatcga tcagggcgtt ggcgtgcgtg
    20701 caatcagcgg tgaaaaaacc ggatttgctt acgctgacca aatcagcctg ctggcgctgg
    20761 aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag tggtgatggc aaagtacaga
    20821 cgctgggcgc ggtagagcat agcccgttgt atacctcggt agatccgctg caaagcatga
    20881 gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa ggttgctcgc gaagcggaca
    20941 agcgcgtaca ggaagtgact gccagcctca gtggtgtcta tgaattaatt ctggttgcgg
    21001 ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt gcgtctttcc gtgagcgttc
    21061 tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg cggcggcggt cgttttagtt
    21121 atgagttctt ccttgccgat cttgacggcg aagtccgtgc ggatgcatgg gcaaaagaag
    21181 cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc accagcgggc accatgccgg
    21241 tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga agcggtaggt cacggtctgg
    21301 aaggcgactt caaccgccgt ggcacttcag tatttagtgg acaggtcggg gagctggtgg
    21361 cttcagaact gtgtaccgtg gttgatgacg gcacgatggt cgatcgccgt ggttcggtgg
    21421 cgattgatga cgaaggtacg ccaggccagt acaacgtgct gattgagaac ggcattctga
    21481 aaggctacat gcaggataaa ctcaacgcgc gtttgatggg gatgacgccg actggcaacg
    21541 gtcgccgtga atcctacgcc catctgccca tgccgcgtat gaccaacacc tatatgctgc
    21601 cgggtaaatc gaccccgcaa gaaattattg aatccgttga gtacggtatc tatgcaccga
    21661 actttggtgg cggtcaggtg gatatcacct ccggcaaatt cgttttctcc acttcagaag
    21721 catatctgat tgaaaacggt aaagtaacga agccggtgaa aggcgcaacg ttgattggtt
    21781 ccggtatcga aaccatgcag cagatttcga tggttggcaa cgacctgaaa ctggataacg
    21841 gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt tggcgtgggc cagccaacgt
    21901 tgaaagtcga taacctgact gttggcggta ctgcgtaata atttcattat tttcagaagg
    21961 ataattaatc tttctacgtg cacgaacggt cccctcgctc ctctggggtt agggtgaggg
    22021 gaacccgttg gcacaggttt gtagaacgta acagtacaat atgaattact tctctttccc
    22081 gcgcccgtgc atctcctgaa acaatttacc gacctcaaca aaataatccg tcagcgagtt
    22141 gatcacgacc tgtaccttca gcggcagctt atctttttcg gtatataacg cataaaccgg
    22201 gcgtggatct gactgataac gcggcagcag gatctccagc tccccacgat tgatctcgtt
    22261 gatcacccac atcagcggca cgtaggcgat cccggcaccc gccgttagcc agcgcaccag
    22321 cgtcatcgga tcattagtca caaatctccc ttgcgggatc aggcgagtcg agatcccttc
    22381 cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg tattcaagcc atgaatggct
    22441 acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg agatagcttt tcgcggcgca
    22501 caccaccatt ggcatcgcgc caagacggcg ggaaaacagg ctggaatcct gcaacgcgcc
    22561 gacgcggatc accacatcca gaccgtcggc aatcaggtcg ggggctggaa ttccggtaac
    22621 cagattggcg ctcaaacctg ggtattcttt cagcattttg gctgtcagcc cggcgagaac
    22681 attttgtgcc atagttgaag aacagccaat gcgtagcgtc ccgatggggg tgttattgaa
    22741 ggcatacagt tgctcatgaa catcctgcac ttcatgaagc atacgacggc agccctggta
    22801 gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg ctacggttta acagctttac
    22861 ctgcaactca tcttccagtt ttgacaccgt ctgactgatg gacgaaacgc tcatctgtag
    22921 ctgtctggcg gcggcggtaa aagagccaaa ttcaactact ttggcaaaca ccgacatgcg
    22981 ttttagtcgt tccattattc actctgactt aaaagtgatt tagatcacat aatatagata
    23041 acagcataac agttacgcta atatattaaa tatcaatcta cagcaatgtt gctctcgccc
    23101 ggctttccat gcccttctct gcggcgacag atgctgaaaa taataacgcc tgctctctct
    23161 atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt ggtgtttggg ctgtccttcc
    23221 caccgatatt ttttgaattg cttttatcac tggcgatttt ctggctggtg cgccgggtac
    23281 ttgtgccaac aggtatctac gactttgtct ggcatccggc gttgttcaac accgcgctct
    23341 attgctgctt gttttatttg atatcgcgac tgttcgtttg aggttgaagt gaaaacacta
    23401 ataagaaaat tctcccgtac ggccatcacg gtcgtattag tcattctggc cttcatcgca
    23461 atttttaatg cctgggtcta ttacaccgaa tccccctgga cgcgtgacgc gcgctttagc
    23521 gctgacgtcg ttgcgatcgc gccggacgtt tctggactca ttacccaggt gaatgttcat
    23581 gataaccagc tggtgaaaaa aggacagata ctgttcacca tcgaccagcc gcgctatcaa
    23641 aaggcgcttg aggaagcgca agccgatgtt gcttattatc aggtactggc acaggagaaa
    23701 cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg cgatgtctcg cgaagagatc
    23761 gaccaggcta acaacgtact acaaacggtt ctgcatcagt tagcgaaagc gcaggcgacc
    23821 cgcgatctgg caaaactgga tcttgaacgc acggtgatcc gagcgccagc agatggctgg
    23881 gtgaccaacc tcaacgtcta taccggtgag tttattactc gaggatcaac ggcggttgcg
    23941 ctggtgaaac agaactcctt ctatgtactg gcctatatgg aagaaactaa gctggaaggg
    24001 gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca gtaacaaagt gctgaaaggg
    24061 actgttgata gtgttgccgc tggggtcacc aacgccagca gcacgcgtga cgacaaaggg
    24121 atggcgacca tcgactctaa ccttgaatgg gtgcgtttgg cgcaacgtgt tccggttcgt
    24181 attcgtctcg acaaccagca agagaacatc tggcctgcgg gcaccactgc tacagtggtg
    24241 gtcactggca aacaagatcg cgacgaaagc caggattcgt tcttccgtaa aatggcccat
    24301 cgcctgcgtg agtttggtta atcacgatgg gtattttctc cattgctaac caacatattc
    24361 gctttgcggt aaaactggcg accgccattg tactggcgct gtttgttggc tttcacttcc
    24421 agctggaaac gccacgctgg gcggtactga cagcggcgat tgttgccgcc ggtccggcct
    24481 ttgctgcggg aggtgaaccg tattctggcg ctattcgcta tcgtggcttt ttgcgcatca
    24541 tcggcacatt tattggctgt attgccggac tggtgatcat cattgcgatg atccgcgcac
    24601 cattattgat gattctggtg tgctgtatct gggccggttt ttgtacctgg atatcctcgc
    24661 tggtacgaat agaaaactcg tacgcgtggg ggctggccgg ttataccgcg ctgatcattg
    24721 tgatcaccat tcagccggaa ccattgctta cgccgcagtt tgccgtcgaa cgttgtagcg
    24781 agatcgttat cggtattgtg tgtgcgatta tggcggattt gctcttttct ccgcgatcga
    24841 tcaaacaaga agtagatcga gagctggaaa gtttgctggt cgcgcaatat caattaatgc
    24901 aactctgtat caagcatggc gatggtgaag ttgtcgataa agcctggggc gacctggtgc
    24961 gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa tatggaatct tcccgctggg
    25021 cgcgggccaa tcgacgttta aaagcgatca atacgctatc gctgacgctg attacccaat
    25081 cctgcgaaac ttatcttatt cagaatacgc gcccggaatt gatcactgat actttccgcg
    25141 aattttttga cacgccggta gaaaccgcgc aggacgtcca caagcagctc aaacgcctgc
    25201 ggagagttat cgcctggacc ggggaacggg aaacgcctgt caccatttat agctgggtcg
    25261 cggcggcaac gcgttatcag cttctcaagc gcggcgttat cagtaacaca aaaatcaacg
    25321 ccaccgaaga agagatcctg caaggcgaac cggaagtaaa agtagagtca gccgaacgtc
    25381 atcatgcaat ggttaacttc tggcgaacca cactttcctg cattctgggc acgcttttct
    25441 ggctgtggac gggctggact tccggcagtg gtgcaatggt gatgattgcg gtcgtgacgt
    25501 cactggcaat gcgtttgccg aatccacgca tggtggcgat cgactttatc tatgggacgc
    25561 tggccgcgct gccgttaggg ctgctctact ttttggtgat tatccctaat acccaacaga
    25621 gcatgttgct gctgtgtatt agcctggcag tgctgggatt cttcctcggt atagaagtac
    25681 agaaacggcg actgggctcg atgggggcac tggccagcac cataaatatt atcgtgctgg
    25741 ataacccgat gactttccat ttcagtcagt ttctcgacag cgcattaggg caaatcgtcg
    25801 gctgtgtgct cgcgttcacc gttattttgc tggtgcggga taaatcgcgc gacaggactg
    25861 gacgtgtact gcttaatcag tttgtttctg ccgctgtttc cgcgatgact accaatgtgg
    25921 cacgtcgtaa agagaaccac ctcccggcac tttatcagca gctgtttttg ctgatgaata
    25981 agttcccagg ggatttgccg aaatttcgcc tggcgctgac gatgattatc gcgcaccagc
    26041 gcctgcgtga tgcgccgatc ccggttaacg aggatttatc ggcgtttcac cgacaaatgc
    26101 gccgcacagc agaccatgtg atatctgccc gtagcgatga taaacgtcgt cggtactttg
    26161 gccagttgct ggaagaactg gaaatctacc aggaaaagct acgcatctgg caagcgccac
    26221 cgcaggtgac ggaaccggtt catcggctgg cggggatgct ccataagtat caacatgcgt
    26281 tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg ttttttcatg gctatactta
    26341 gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc aacagatgaa tatttatgcc
    26401 tttgatttta atgagattga gagtcaggag gatttttatc gtgactttag ccaaaccctt
    26461 ggtctggcta aagataaggt acgcgatctt gactcactat gggatgtgtt aatgaacgat
    26521 gtcctgccgc taccacttga gattgaattt gttcatctgg gagagaaaac gcgtcgccgt
    26581 tttggcgcgt taatattgct gtttgatgag gcagaggaag agctggaagg gcatttgcgt
    26641 tttaatgttc gtcattagcg caaaaaaaag cccccgaacc gggggcaata tcgtcggaca
    26701 aaacgatgag ggtttatttg tacagttcag ccgtagcgtg ccaggtgtca ccgctacgag
    26761 cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc ttttttgttc agcatttcac
    26821 gtatatccat tggcgaagac gccacaccac ttacggatac ggtcccgatt gcttcacgat
    26881 tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc accgaaagag agaacagaaa
    26941 gtacgcttaa tgcagctaca gtggttttga ttttcatgat ttttacctcg acataatctt
    27001 ttagctgggc ctttgtttcg tgacccatat cacaaaatca agcatacact aattattgaa
    27061 ctaattaata ccacgctaac tgtttcagtt gatactaagt gttaaaaatg tgaattttaa
    27121 taacaaaaaa gtataaaaaa cacggttatt tattattaat tatagtattt tcaattagat
    27181 aatgaatttt gcataatgtg ctttttttac cgtatgtatt cattttgtga atgatgtgtc
    27241 gcggtaaaac gcactattcg ttaaatgtat cgcaggagaa agaaggggaa tgagagggat
    27301 aagcaacatt ttccccgccg ccagaagcga cggggcagag attaaagctc ctggtcgaac
    27361 agctctaaaa tcgcttcgta caggtctttg acggtgaaac cgttagcagg ggtggtaaag
    27421 atggtgtcat cgccagcgat ggtgcccaga ataccttctg ctttgcccag tgagtccagc
    27481 aggcgagcaa ttaactgcgc cgcgccaggg ctggtatgaa tcacgacaac tgcatcgttg
    27541 tagtcgatat ccagcaccag attcttcaat ggactggagg tggttggtac acccagttca
    27601 gctggcaggc agtaaaccat ttccattttg gcattgcgtg tacgtacagc accaaacttg
    27661 gtcaacatcc gcgagacttt agactgatta atattgtcaa agccttgctc ctgcaacgcg
    27721 gcgacgattt cgccctggga gctaaatttc tcttctttaa gtaatgcttt aaatgcttta
    27781 actagttctt cttgcttagc cgagcttcgc ataagtcacc cgatatggtg gttgatacaa
    27841 cattattgtg catacagatg aatttttatg caaacagtca gccctgaaga aggctgaaat
    27901 aatgttatga aagagcggga ttttatcaaa tttcgttatt gagaaacatg cctgcgtcac
    27961 ggcatgcaaa ttctgtttaa aagtaaatta attgttatca aattgatgtt gttttggctg
    28021 aacggtaggg tatattgtca ccacctgttg gaatgttgcg ctaatgcata agcgactgtt
    28081 aattacgtaa gttaggttcc tgattacggc aattaaatgc ataaacgcta aacttgcgtg
    28141 actacacatt cttgagatgt ggtcattgta aacggcaatt ttgtggatta aggtcgcggc
    28201 agcggagcaa catatcttag tttatcaata taataaggag tttaggatga aagtcgcagt
    28261 cctcggcgct gctggcggta ttggccaggc gcttgcacta ctgttaaaaa cccaactgcc
    28321 ttcaggttca gaactctctc tgtatgatat cgctccagtg actcccggtg tggccgtcga
    28381 tctgagccat atccctactg ctgtgaaaat caaaggtttt tctggtgaag atgcgactcc
    28441 ggcgctggaa ggcgcagatg tcgttcttat ctctgcaggc gtagcgcgta aaccgggtat
    28501 ggatcgttcc gacctgttta acgttaacgc cggcatcgtg aaaaacctgg tacagcaagt
    28561 tgcgaaaaac tgcccgaaag cgtgcattgg tattatcact aacccggtta acaccacagt
    28621 tgcaattgct gctgaagtgc tgaaaaaagc cggtgtttat gacaaaaaca aactgttcgg
    28681 cgttaccacg ctggatatca ttcgttccaa cacctttgtt gcggaactga aaggcaaaca
    28741 gccaggcgaa gttgaagtgc cggttattgg cggtcactct ggtgttacca ttctgccgct
    28801 gctgtcacag gttcctggcg ttagttttac cgagcaggaa gtggctgatc tgaccaaacg
    28861 tatccagaac gcgggtactg aggtggttga agcgaaagcc ggtggcgggt ctgcaaccct
    28921 gtctatgggc caggcagctg cacgttttgg tctgtctctg gttcgtgcac tgcagggcga
    28981 acaaggcgtt gtcgaatgtg cctacgttga aggcgacggt cagtacgccc gtttcttctc
    29041 tcaaccgctg ctgctgggta aaaacggcgt ggaagagcgt aaatctatcg gtaccctgag
    29101 cgcatttgaa cagaacgcgc tggaaggtat gctggatacg ctcaagaaag atatcgccct
    29161 gggcgaagag ttcgttaata agtaattgat tagcgaataa taaaaaaccg gagcacagac
    29221 tccggttttt gttttgagca ctcgacttaa ttggttgccg gatattcctg aatggtgacc
    29281 tgcagcgtta actgcttatc atcacgcatc actacaaccg ggatcaccga accagggcga
    29341 atttccgcca cctgatccat cgtctccaga gcagagatgg ccggtttgtt atccaccgaa
    29401 ataatcagat cgttgacctg aatacccgca ttcgccgctg ggccgtcagg tgacacttca
    29461 ttaaccacga tcccttgcag ttgatctata ccaccgctct gcgcgtgcag tggtgcgatc
    29521 tcgcgtccgc cgataccaat gtagccgcgg atcacgcgac catcgcggat cagcttatcc
    29581 ataattttgg ttgctaactg gaaaggaatc gcaaagccga taccttccgg cgtttcgcca
    29641 tcgttactct tatcaaacga cagcgtgtta atgcccatca gttctcccag cgagttcacc
    29701 agcgcgccgc cagagttacc gtggttaatg gaagcatcgg tttgtaggaa gttttgccgc
    29761 ccggtcgggt tcagaccgat tcgacccgtg gcactaataa tcccttgggt aatggtctgc
    29821 ccgaggttgt acgggttacc gatcgccagt actacgtcgc caatgtgcgg tacgcgacgt
    29881 gcattaattg gaatggtagg taaaccgcca gtggcattaa ttttaagtac cgccagatcg
    29941 gttagagagt cagatcccac cagcaatgct tcaaatacac gtccatcctg taaggcgacg
    30001 atgatctgat cggcgtcgtt gatgacgtgt ttattggtga tgatataacc gcgttgatcc
    30061 atgattacac cggatcccag ggtgcggatc tcaagctggt tgtgagagtt ggtgttcaaa
    30121 ccacggttgt aaacgttaac caccgctggc gcggcgcggc gaaccgccag attatagctg
    30181 gcaggcgtct catcggtact gtcaaattgc ggagtggaaa gcgggttaag gctgcgcagc
    30241 gaaggcatgg caaccagcag aatagcgccg acaattaatc caatcgcaac ggaacgtaag
    30301 agcttcacaa acatgatgga ggcgtcatta aaaaagggaa cggcagcagc ataccacgag
    30361 ttaaccggac atcacacgtc agcctgatgc ccggtttacg acattaacgc atcagcagat
    30421 agatgctttc attgccgcgt acaatttgca gggcgatgat ggccggtttt gccgccagca
    30481 ctttacgcat ttcagcaatc gagttcaccc gatcgcggtt gacgccaatg atcacatcgt
    30541 ctttttgcaa gccagcctga gcagctgggc ttcctttgac aacttcatct atcttaatac
    30601 ctttgccgcc atcttttagc tgaccatcgc tcaacgttgc accttccagc gctggcgtga
    30661 tcatttcagc gctggccgac gaagaggtgt tggtatcaag cgtcacttct acttccagtg
    30721 gtttgccgtt acgcagcagg ccaagcttca ctttcgtgcc cggctctgta gtcgcgatac
    30781 gagagcgcaa ctcagcaaag ctattcagcg gtttgccgtt gaggctggta ataatatcgc
    30841 ccgctttgac gcccgctttc gctgagccag aacttggcaa cacttcgctg acaaacgcgc
    30901 cacgctgcac gtcaaggttg aaggctttgg cgatatcggc actcatctcg gtgcctttga
    30961 tgcctaacaa accgcgtttg atttcaccaa agtcgataag ctgctgcgcc agtgttcgcg
    31021 ccatattact ggggatggca aatccaatcc cgacgctccc gccgccaggc gcaaggattg
    31081 cagtgttgat gccaattaac tcaccgttaa ggtttaatag tgcaccgccg gagttaccgc
    31141 ggttaatgga agcatctgtc tggataaagt tttccagacc ttcaagattc aacccgctgc
    31201 ggcctaatgc ggaaacaatg ccagaggtgg cggtttgccc aaggccaaat gggttaccga
    31261 ccgctacggc aaaatcaccg acgcgcaatt tatcggagtc ggcaatagcg atttgcgtta
    31321 atttgctcgg gttttgaatt tgtaacaggg cgatatcgct ctggtcatcg ctaccaatca
    31381 gtttttcatc aaactcgcgc ccatcattga gctgaatact gattttctgt gcctgattaa
    31441 tcacatggtt gttggtcagc acatagcctt tactggcgtt gatgatgaca ccggaaccta
    31501 aaccttcgaa gggttgtgca ggttgatccg gtaaatcatc accaaaaaac tttttgaatt
    31561 cttccgggat tttctgtccc tgactggccg ttccttccac ccgtacgctc accactgccg
    31621 gaagcacttt ttccagcatt ggagccagac tggggagagg ggcctgatcg gcaacctggc
    31681 ctggaatcga cgcgacggcc tgaaatgacg ccgagagagt taacccggca cttaacgcta
    31741 atgcactcaa cagctgggtt tgttttttca ttattcctgc tctcgtacct gaatgataag
    31801 aaaagagatt caaaacagtt tgatgttatt gaattttcag acggctaaca atgagacacc
    31861 agattaaatg atagtcgtgg agggagagaa gaggagggcg caatggctgc gcccgaaaaa
    31921 taaattagtc gcgcttcgcg ccagtacgca gcaggccgga tgcgccttca gaatagtcgc
    31981 gaggcatctg caccggtgcc tgatcgttgc tggcttcaga ctctgccaga cgattacgga
    32041 acgggtttgc ttcagcagac agttccggca gcaggctgct ggagcttttt gccatgtgct
    32101 gatacagctg gcgatagtcg tgcgccatgg tatccagtaa ttccgcgctg cgggcaaagt
    32161 ggctaaccag ctcttcgcga tactcgtcca gttcagcttt attcttttcc agttcgtact
    32221 gcaacgcctg ttgctggcgt agtttacgat taccaaaacg catggccaca gcaccaataa
    32281 tgatgccgac gactaaccca attagcgcat attcccaggt catgaacatc tcccgttgtc
    32341 ttttgtttcc gtagggtgtt ggcttcaggc tccatgcctg cggctgatta tgccactata
    32401 accgttaatt ccacagaagt ggaatcccga ctgcatatcg cgtagtgtag aacggccttt
    32461 ttttcgtcaa ccgtgaacaa cggcgcaccg attattcaag gaataacaat aagatcatgc
    32521 aaagcgttac cccaacatcg caatacctga aggcgctcaa tgaaggcagc catcaacccg
    32581 acgacgttca aaaagaggcc gtcagccgcc tggaaattat ttatcaagaa ctcatcaata
    32641 gcaggccacc agcccccagg acgagtgggc taatggcgcg ggtcggtaag ctgtggggta
    32701 aacgcgaaga cacaaagcat acgccagtgc gtggcttata tatgtggggc ggtgtaggac
    32761 gcgggaaaac ctggctgatg gaccttttct atcaaagcct gccgggagag cggaaacagc
    32821 gcctgcactt tcaccgtttt atgctgcggg tgcacgaaga gctaactgcc ttacaggggc
    32881 agaccgatcc gctggaaatt attgccgatc gctttaaagc cgaaactgac gtgctctgtt
    32941 ttgacgaatt ttttgtttct gatattaccg acgccatgct acttggcggt ctgatgaaag
    33001 ccctgttcac ccgcggtatt accctggtag cgacgtcaaa tattccgccg gacgaacttt
    33061 atcgaaatgg cctgcaacgt gcgcgttttc tgcctgcaat cgatgccatt aaacagcatt
    33121 gtgatgtaat gaacgtggac gctggtgttg attatcgact gcgtacactc actcaggcgc
    33181 atctgtggct ttcgccactc aacgatgaaa cccgggcgca gatggataaa ctatggttgg
    33241 cgctggcggg ggtgaaacga gaaaattcac cgacgttaga aatcaaccat cggccattgg
    33301 cgacaatggg cgtcgagaac cagacgctgg cggtttcttt tactacgctg tgcgtcgacg
    33361 cccgcagtca gcatgactat attgcgctct cacgcctgtt tcacacagta atgttgtttg
    33421 atgtgccagt catgacgcgg ttgatggaga gcgaagcgcg gcgctttatt gcgctggtgg
    33481 atgagtttta cgagcgccat gtcaaattag tggtgagtgc agaagtgccg ctgtatgaaa
    33541 tttatcaggg tgagcggctg aagtttgagt tccagcgttg cctgtcacgt ctgcaagaga
    33601 tgcaaagcga agagtatctg aagcgcgagc atttggcggg ttaaaacctg ccacaaatca
    33661 caaaaagggg tcgatctttg accccgactt ctctataatc ctgcgacccc acgttacaag
    33721 aaagtttttt tcccaaaact ttttgtgtgc tggcataggc tattcgaagg ggtaggtttg
    33781 ccggactttg tcgtgtgaac ctcaacaatt gaagacgttt gggtgttcac caacgtgtaa
    33841 ctatttattg ggtaagcttt taatgaaaac ttttacagct aaaccagaaa ccgtaaaacg
    33901 cgactggtat gttgttgacg cgaccggtaa aactctgggc cgtctggcta ctgaactggc
    33961 tcgtcgcctg cgtggtaagc acaaagcgga atatactccg cacgtagata ccggtgatta
    34021 catcatcgtt ctgaacgctg acaaagttgc tgtaaccggc aacaagcgta ctgacaaagt
    34081 gtactatcac cacaccggcc acatcggtgg tatcaaacaa gcgacctttg aagagatgat
    34141 tgctcgccgt cctgagcgtg tgattgaaat cgcggttaaa ggcatgttgc caaaaggccc
    34201 gctgggtcgt gctatgttcc gtaaactgaa agtttacgcg ggtaacgagc acaaccacgc
    34261 ggcacagcaa ccgcaagttc ttgacatcta atcgggatta taggcaatgg ctgaaaatca
    34321 atactacggc actggtcgcc gcaaaagttc cgcagctcgc gttttcatca aaccgggcaa
    34381 cggtaaaatc gtaatcaacc aacgttctct ggaacagtac ttcggtcgtg aaactgcccg
    34441 catggtagtt cgtcagccgc tggaactggt cgacatggtt gagaaactgg acctgtacat
    34501 caccgttaaa ggtggtggta tctctggtca ggctggtgcg atccgtcacg gtatcacccg
    34561 cgctctgatg gaatacgacg agtccctgcg ttctgaactg cgtaaagctg gcttcgttac
    34621 tcgtgacgct cgtcaggttg aacgtaagaa agtcggtctg cgtaaagcac gtcgtcgtcc
    34681 gcagttctcc aaacgttaat tggcttctgc tccggcagaa aacaatttgc gaaaaaaccc
    34741 gcttcggcgg gtttttttat ggctaaattc tgaatcagcg taaaaaatgg gaagttgctt
    34801 tttgctgccg tctgaaagac aggtaaaaca aaaaccacat cgccaataag ggtctaagtc
    34861 aactatttca gactaaagcg catctctttt tccccatttc cggcatccac tcaccacaaa
    34921 ggtcgcaaaa tctggtaaac tatcatccaa ttttctgccc aaatgtcggg tattgctcat
    34981 tttttgtttg attttcgaac aaagagagtc gttccttatt gggtaacaca acttctgact
    35041 ggccacctgg tggctggtag cagtaaaaat tctgactata cctggaggtt ttcatggctg
    35101 tcgctgccaa caaacgttcg gtaatgacgc tgttttccgg tcctactgac atctatagcc
    35161 atcaggtccg cattgtgctg gctgagaaag gtgtaagttt cgagatcgaa cacgtggaaa
    35221 aggacaatcc gcctcaggat ctgattgacc ttaacccgaa tcagagcgtt ccgaccctgg
    35281 tggatcgtga gctgaccctg tgggaatctc gcatcattat ggaatatctg gatgagcgtt
    35341 tcccgcatcc gccactgatg cctgtttacc cggtagctcg cggtgaaagc cgtctgtaca
    35401 tgcatcgcat cgaaaaagac tggtacacgc tgataaacac tatcatcaac ggttcagctt
    35461 ctgaagcaga tgccgcacgt aagcaactgc gcgaagaact gctggcgatt gcgccggtct
    35521 tcggtcagaa gccgtacttc ctgagcgatg agttcagcct ggtcgattgc taccttgctc
    35581 cgctgctgtg gcgtctgccg caactgggca tcgagttcag cggcccgggt gcgaaagagc
    35641 tgaaaggcta tatgacccgc gtctttgagc gtgactcttt ccttgcttct ttaactgaag
    35701 cagaacgtga aatgcgtctg ggccggagtt aatctgtatg gatttgtcac agctaacacc
    35761 acgtcgtccc tatctgctgc gtgcattcta tgagtggttg ctggataacc agctcacgcc
    35821 gcacttggtg gtggatgtga cgctccctgg cgtgcaggtt cctatggaat atgcgcgtga
    35881 cgggcaaatc gtactcaaca ttgcgccgcg tgctgtcggc aatctggaac tggcgaatga
    35941 tgaggtgcgc tttaacgcgc gctttggtgg cattccgcgt caggtttctg tgccgctggc
    36001 tgccgtgctg gctatctacg cccgtgaaaa tggcgcaggc acgatgtttg agcctgaagc
    36061 tgcctacgat gaagatacca gcatcatgaa tgatgaagag gcatcggcag acaacgaaac
    36121 cgttatgtcg gttattgatg gcgacaagcc agatcacgat gatgacactc atcctgacga
    36181 tgaacctccg cagccaccac gcggtggtcg accggcatta cgcgttgtga agtaatacaa
    36241 aacaggccca ggcggcctgt tttgtctttt taatgactta ataaaatagc gtgattgtgg
    36301 caatcatatt caccacgaaa ccgactgcca tggggatcgc tgtccgcttc accaccgcaa
    36361 aaggcgaaac gcctgcaatt cctgacgtaa cgaccaccac cgcagtaatc ggcgaaaccg
    36421 cgcgcgctag cgtcgtggca aaatgcatcg gcataatcat tacaaccgct ggtacatgta
    36481 gtccggctgc gatattcgga ataagactgg caaatgacat aaacggcgca ttgccagagc
    36541 ccatcacaat ggcacaaatg gcaatgacta gcgccataat aatcatcacg ccaataccgc
    36601 ccagaccaga atgctccgca cccctgataa ccgcatcaac agtgccaatc gtcgttaagc
    36661 ctttcgcaaa tatttcaccc gcgaccacca gcgttaccac gttgacaaac tgcgtaccca
    36721 tgccgtcaaa aaacgcctgc acatcgtcca ttgtttcgcg caagttatgc ttgcggaaga
    36781 actcaacaaa catcgtcacc gtcaaactta gtaacatcac caccaccaga tgcagttctg
    36841 acttcatcag cccgacgtgg gcgaggaaca gcgagccgag catcaggatt aacggcatca
    36901 caggtaaaat ggcgtaatag agcggcggga cattatcgag agttttttgc tctgcctgtt
    36961 cgtgattgat atttttatct tttttgtcaa aagcgcgttg cacgaaaaag tgggagatcg
    37021 ccaccgagat aatgacgcaa gaggcgaccg gaagctggta gtggaagaag tatgtggcaa
    37081 ttttcattcc cgcgacctgg gctgcaaaaa tggagttcgt ttccagaatc ccccactcaa
    37141 tggacatcgt ggttgcgata accgccaccg cagagaggcg acttactccc agactcacca
    37201 gcgtcggaaa taaggtgacc atcagcaaca tacccagacc ggaggcgctg gtaataaact
    37261 gcgccatgat ttggccgatg acgtaagttg ccgacagaat aatatacggc gagcgaatga
    37321 gttttaacgg gcggcttagc aagctcacca tcgcgcgact ggctcctatg cgctccatgt
    37381 agcgggcata accgcccacc gccataatcg acagccccaa tccggcaatg cggttggaca
    37441 gcatgcgcag gatttcatta taaatgtcga cgatcaagta gccgctgctt ttggcgggat
    37501 cgagtacacc accgaaccct aaccagactc cgcacatcat caggaagaca cctgcggcag
    37561 ccagcaccac ctgaggtttg tagtttttca ggatcaaata gcccatcgta attaatacga
    37621 tgacagatat aattatgccg aac
//
LOCUS       DADLTF010000020        36495 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.020, whole genome shotgun sequence.
ACCESSION   DADLTF010000020 DADLTF010000000
VERSION     DADLTF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.020"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="JN749_001000"
     CDS             complement(<1..170)
                     /gene="ygdR"
                     /locus_tag="JN749_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HAY7367496.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQG"
     gene            complement(308..1021)
                     /locus_tag="JN749_001001"
     CDS             complement(308..1021)
                     /locus_tag="JN749_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7367497.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="JN749_001002"
     CDS             complement(1090..1779)
                     /gene="mutH"
                     /locus_tag="JN749_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HAY7367498.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            2464..2994
                     /gene="rppH"
                     /locus_tag="JN749_001003"
     CDS             2464..2994
                     /gene="rppH"
                     /locus_tag="JN749_001003"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HAY7367499.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            3007..5253
                     /gene="ptsP"
                     /locus_tag="JN749_001004"
     CDS             3007..5253
                     /gene="ptsP"
                     /locus_tag="JN749_001004"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7367500.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            5404..6279
                     /gene="lgt"
                     /locus_tag="JN749_001005"
     CDS             5404..6279
                     /gene="lgt"
                     /locus_tag="JN749_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAY7367501.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINSELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            6286..7080
                     /gene="thyA"
                     /locus_tag="JN749_001006"
     CDS             6286..7080
                     /gene="thyA"
                     /locus_tag="JN749_001006"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAY7367502.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            7264..7734
                     /locus_tag="JN749_001007"
                     /pseudo
     CDS             7264..7734
                     /locus_tag="JN749_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            7725..8288
                     /locus_tag="JN749_001008"
     CDS             7725..8288
                     /locus_tag="JN749_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HAY7367503.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            8285..8692
                     /locus_tag="JN749_001009"
     CDS             8285..8692
                     /locus_tag="JN749_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HAY7367504.1"
                     /translation="MNREKGVSSQALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            8677..9000
                     /locus_tag="JN749_001010"
     CDS             8677..9000
                     /locus_tag="JN749_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAY7367505.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            9013..12381
                     /gene="recC"
                     /locus_tag="JN749_001011"
     CDS             9013..12381
                     /gene="recC"
                     /locus_tag="JN749_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HAY7367506.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            12557..15445
                     /gene="ptrA"
                     /locus_tag="JN749_001012"
     CDS             12557..15445
                     /gene="ptrA"
                     /locus_tag="JN749_001012"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HAY7367507.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRMADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            15438..18980
                     /gene="recB"
                     /locus_tag="JN749_001013"
     CDS             15438..18980
                     /gene="recB"
                     /locus_tag="JN749_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HAY7367508.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            18980..20806
                     /gene="recD"
                     /locus_tag="JN749_001014"
     CDS             18980..20806
                     /gene="recD"
                     /locus_tag="JN749_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HAY7367509.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(20868..22199)
                     /gene="argA"
                     /locus_tag="JN749_001015"
     CDS             complement(20868..22199)
                     /gene="argA"
                     /locus_tag="JN749_001015"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HAY7367510.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            22431..23684
                     /locus_tag="JN749_001016"
     CDS             22431..23684
                     /locus_tag="JN749_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HAY7367511.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            complement(23759..23835)
                     /locus_tag="JN749_001017"
     tRNA            complement(23759..23835)
                     /locus_tag="JN749_001017"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23799..23801),aa:Met,seq:cat)
     gene            24044..25141
                     /gene="mltA"
                     /locus_tag="JN749_001018"
     CDS             24044..25141
                     /gene="mltA"
                     /locus_tag="JN749_001018"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HAY7367512.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            25380..26186
                     /gene="tcdA"
                     /locus_tag="JN749_001019"
     CDS             25380..26186
                     /gene="tcdA"
                     /locus_tag="JN749_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HAY7367513.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="JN749_001020"
     CDS             complement(26237..26680)
                     /gene="csdE"
                     /locus_tag="JN749_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HAY7367514.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="JN749_001021"
     CDS             complement(26680..27885)
                     /gene="csdA"
                     /locus_tag="JN749_001021"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HAY7367515.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            28077..28304
                     /locus_tag="JN749_001022"
     CDS             28077..28304
                     /locus_tag="JN749_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HAY7367516.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            28655..29572
                     /locus_tag="JN749_001023"
     CDS             28655..29572
                     /locus_tag="JN749_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HAY7367517.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            29591..29986
                     /locus_tag="JN749_001024"
     CDS             29591..29986
                     /locus_tag="JN749_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HAY7367518.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            29979..31079
                     /gene="rlmM"
                     /locus_tag="JN749_001025"
     CDS             29979..31079
                     /gene="rlmM"
                     /locus_tag="JN749_001025"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HAY7367519.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="JN749_001026"
     CDS             complement(31123..31854)
                     /gene="fucR"
                     /locus_tag="JN749_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HAY7367520.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSSALFHIRLKSHYAHKADIAREALAWIEERMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="JN749_001027"
     CDS             complement(31912..32334)
                     /gene="fucU"
                     /locus_tag="JN749_001027"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HAY7367521.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="JN749_001028"
                     /pseudo
     CDS             complement(32336..33755)
                     /gene="fucK"
                     /locus_tag="JN749_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="JN749_001029"
     CDS             complement(33864..35639)
                     /gene="fucI"
                     /locus_tag="JN749_001029"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HAY7367522.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(35672..>36495)
                     /gene="fucP"
                     /locus_tag="JN749_001030"
     CDS             complement(35672..>36495)
                     /gene="fucP"
                     /locus_tag="JN749_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HAY7367523.1"
                     /translation="AIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQ
                     TPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQ
                     FCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPH
                     KVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYG
                     SSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         8682 a         9104 c         9894 g         8815 t
ORIGIN      
        1 ttaccttgct gatcgtgata actcaccagc ccggtatcat catcaatttc aggttttcca
       61 tcggtcaaaa tcatacggcc atctttggtc gccatgacgt aatcactgga acacccggaa
      121 acagcaaacg ccagtccgac tgcagaaatt attactgccc attttttcat ccggttatcc
      181 tcatttggcc tacctatgat aatagtctgt ttataaatcg acgttatata tcttgaaagc
      241 aggaaaatct taaactgtga gggtaaaagg agtgagtacg ccctcccgca ggagagcgta
      301 cggagtatca gagcggattc tttttgttgc gaatcaagtt gaggctttca accgcaatag
      361 agaagaacat cgcgaagtag atgtaacctt tcggtacgtg gatgtcgaaa ctttccagaa
      421 tcagggtaaa gcccaccaga atcaggaaag agagcgccag catttttacc gaaggatggc
      481 gttcgacaaa atcaccaatc gagcgcgcgg cgaacatcat cacgcctacg gcaatcacca
      541 cggctgccat cataataaac aggtgatctg acagacccac agcggtaatc accgagtcga
      601 ggctgaagat aatatccagc agcataatct gcacgatagc gccgaggaat gatgaaacgc
      661 gtgttttcag cccttcttct tcaccttcaa tggattcgtg gatttccttg ctggctttcc
      721 agataaggaa caagccaccc agaagcagaa tcaaatcacg ggcagaaatt tcctgactga
      781 atattgtaaa aagcggattt gtcaggcgcg taacccaaga gattgatgcc agcagagcca
      841 gacgcataac catggctccc gccaacccca gacggcgcgc atgagcacgt tgtgctgtgg
      901 gaagctttgc caccaccaga gaaaggaaaa taatattgtc gatcccaaga acgatctcca
      961 gcagcgtcag cgtaccgagc gcaagccagg cgttaggatc ggttatccat gcaaataaca
     1021 tctaaaaagc cctgccaaaa aaatgaagcg gtaattatat gcccggaaag cggcaggtca
     1081 aagcgatggc tactggatca gaaaatgacg ggccagtagt gcactggtga aattcttctt
     1141 caaataaaag ccgcgtggca gcgtcagaat ccgttcgccc cgggcaccaa tggcttcggt
     1201 aagcgctttc gcattcgctg ctttcggtct tatctgtaaa tattccccgt gacgagcggt
     1261 gatccgctca acctgaccga gaacaatcat atccattaat tcttcccagt cttcgcgtag
     1321 ctgtcggtct tcctcttcat tcgggctcca cagcaacggt gatcctacgc gacgctgcgc
     1381 cagcgggatg ctgcgctcgc cttcaaccgg tatccacagt acgcgtttga gcttgtggcg
     1441 cacatggctg gtttcccagg tcaccccgct attacccgtt aacggggcaa cacaaacgaa
     1501 tgttgtttcc agcggacgac caagactatc cacagggata gttttaagtt ccacgcccag
     1561 agcagcaaaa tcttgctcag gtttactccc tgcgctggca cctagccaga tctccagtaa
     1621 cacgccaatc cagcccttat cgcgttttaa attctctggc gtaaccagcc cggcaagtgc
     1681 cgccagttct cccaatgtat aaccagaaag ttgctgtgct tgcgctaaca actgttcttc
     1741 agtttcggga ggagagagca gtgggcgagg ttgggacatg tcatgatgcc ttggttaaaa
     1801 aacaaacagc aattatgtgc cgaatattcg cagtgtaact ttttccacat gtgttgttca
     1861 attctctgat ttatattgct ttttaggctg ccggtaatcg agttgcgcaa cactgcaata
     1921 ggttttccaa atctggtcac tgaaaataaa caggatctta caccatgtta tccacagaaa
     1981 gctgggataa ctgtgaaaaa cctccactac tgttttcatt tacagccttg acgtgcgacg
     2041 aaactccagt ttttagtggt tcagtgctta acttagtggc acattctgtg gataaatacg
     2101 gcattggttg atctttcgcc aacccgtaaa tgagaggtgt gacctgggtc acgaattact
     2161 ctttttcatt actcaaatta catgtaatgc attgactaat aatgaatatt ttaaaatgtc
     2221 tatatcgggc ttattcagaa tgctccgggt tttcccgtag taattccgta gttcttcaca
     2281 actctatcca cagaaaaggt gaataaaaac ggctatccac cccttcctct gtttataact
     2341 ctgcacataa ctgtgagtta ttcaattgtt attagtgtgt aatgccctgc agagagtggt
     2401 ttaccgtctt cctggagtat gaaacaatca ttcgtatata aagctttatt ttgaggtagt
     2461 ccggtgattg atgacgatgg ctaccgccca aacgtaggta tcgtgatttg taatcgccag
     2521 gggcaggtaa tgtgggcccg gcgatttggt cagcactcct ggcaatttcc gcaaggcgga
     2581 atcaaccccg gagaatccgc agagcaggcg atgtaccgtg aattgtttga agaagtagga
     2641 ttaagccgca aagacgttcg aatccttgct tcaacgcgta actggttgcg ctacaaatta
     2701 ccgaaacgtt tggtgcgttg ggacacgaag ccggtttgta tcggccaaaa acaaaaatgg
     2761 tttctcttgc agctggtaag cggcgatgca gaaatcaata tgcaaaccag cagtacacca
     2821 gagtttgacg gctggcgatg ggtaagttac tggtatccgg tcagacaggt ggtgtcattt
     2881 aaacgtgatg tctaccgtag ggtaatgaaa gagttcgcga gtgtggtgat gtcactgcag
     2941 gaaaatacgc caaaaccaca aaacgcatct gcttatcgac gtaaaagagg ttaagtcacg
     3001 ccaattatgc tcactcgcct gcgcgaaata gtcgaaaagg tagccagcgc accacgcctg
     3061 aatgaggcgt taaatattct ggttaccgac atctgtcttg cgatggatac cgaggtctgt
     3121 tcggtctacc tggccgatca tgatcgacgt tgttactacc tgatggcgac ccgggggctg
     3181 aaaaaaccac gcggtcgcac tgtaacgctc gcgtttgatg aagggatcgt cggcctggtt
     3241 ggcaggctgg cggaaccgat aaaccttgca gatgcgcaaa agcaccccag cttcaaatac
     3301 atcccctccg taaaagaaga acgtttccgc gcgtttttag gcgtaccaat tattcaacgt
     3361 cgccagttgc ttggtgtact ggtggtacag caacgagagt tgcgccagta tgacgaaagt
     3421 gaagaatcct tcctggtgac gcttgccacc cagatggcag ctattctttc tcagtcgcag
     3481 ttgactgccc tgtttgggca atatcgccag acgcgaatcc gcgcattacc ggcagcacct
     3541 ggtgtggcga ttgccgaagg ctggcaggat gccacgttac ctttaatgga acaggtgtat
     3601 caggcatcaa cgctggatcc ggctctggaa cgcgaacgac tgaccggggc gctggaagaa
     3661 gcggcaaacg agtttcgccg ctacagcaaa cgctttgccg ccggagcaca aaaagaaacg
     3721 gcggctattt tcgatcttta ctcgcacctg ctttcggaca cccggctgcg tcgcgaattg
     3781 tttgccgagg ttgataaagg ctcggtggca gagtgggcgg taaaaacggt cattgaaaaa
     3841 tttgccgaac agtttgccgc gctaagcgat aactatctca aagagcgggc tggcgattta
     3901 cgtgcgctgg gtcagcgatt gctgtttcat cttgatgacg ctaatcaagg gccgaacgcc
     3961 tggccggaac gtttcattct ggtggcagat gaactgtcag cgacaactct tgctgagctg
     4021 ccccaggatc gcttagtcgg tgttgtcgtg cgagatggcg cagccaactc ccatgctgcg
     4081 atcatggtac gtgcgctggg gatccctacc gtgatgggcg cggatattca gccttcggtg
     4141 ctgcatcgta ggacgctgat cgttgatggc tatcgcggtg aattgctggt cgatccggag
     4201 ccggtactgc tgcaagaata tcagcggcta attagtgaag agattgagct tagccgtctg
     4261 gcggaagatg acgtcaattt acccgcgcag ttaaaaagcg gtgagcgtat aaaagtcatg
     4321 ctcaatgctg gtttaagccc ggaacatgaa gaaaaactgg gcagccgtat tgatggcatc
     4381 ggtctttatc gcactgaaat cccattcatg ctgcaaagtg gtttcccgtc ggaagaagaa
     4441 caggtggcgc agtatcaggg gatgctgcaa atgttcaatg ataaacccgt caccttgcgt
     4501 acgctggatg tcggagcaga taagcagctg ccttacatgc cgatcagcga agagaatcca
     4561 tgcctgggtt ggcgtggtat tcgcattacg ctcgatcagc cggagatctt cttgatccag
     4621 gtgcgggcga tgctgcgtgc taatgccgct acgggcaacc tgaatattct gttgccgatg
     4681 gtcaccagcc tcgatgaagt tgacgaagca cgccgcctga ttgaacgtgc cggacgtgaa
     4741 gtcgaggaga tgatcggtta cgaaattccc aaaccacgta tcggcatcat gctggaagtg
     4801 ccgtcaatgg tatttatgct gccgcatctg gcaaagcggg tcgatttcat ctctgttggc
     4861 accaacgatc tgactcaata cattctggcc gttgatcgca acaatacccg ggtggctaac
     4921 atttacgaca gtcttcatcc tgcaatgtta cgagctctgg cgatgatcgc ccgggaagcg
     4981 gaaatacatg gtatcgatct ccgtttgtgc ggtgaaatgg cgggcgatcc catgtgtgtg
     5041 gcaatcctca ttgggcttgg gtatcgccat ctgtctatga acggacgttc tgtagcgcgg
     5101 gtaaaatacc tgctgcggcg cattgatttt gccgaagcag aaaatcttgc gcagcgtagt
     5161 ctggaagcgc aactggcgac cgaagttcgt catcaggttg cagcctttat ggagcgtcgc
     5221 ggcatgggcg ggctgattcg cggagggtta tagcgcggat catatacata tcttttaacg
     5281 gtatccggca accagccagg tccccttgtg ctattattcg cacctttgga gcgcctgaaa
     5341 cctgcggcgc gcatttcaat cgctgttctc tttcagcgaa ataacaagaa cttgtggtga
     5401 cagatgacca gtagctatct gcattttccg gagtttgatc cggtcatttt ctcaatagga
     5461 cccgtggcgc ttcactggta cggcctgatg tatctggtgg gtttcatttt tgcaatgtgg
     5521 ctggcaacac gtcgggcgaa tcgtccgggc agtggctgga ccaaaaatga agttgaaaac
     5581 ttactctatg cgggcttcct cggcgtcttc ctcgggggac gtattggtta tgttctgttc
     5641 tacaatttcc cgcagtttat ggccgatccg ctgtatctgt tccgtgtctg ggacggcggc
     5701 atgtctttcc acggcggcct gattggcgtt atcgtggtga tgattatctt cgcccgccgt
     5761 actaaacgtt ccttcttcca ggtctctgat tttatcgcac cactcattcc gtttggtctt
     5821 ggtgccgggc gtctgggcaa ctttattaac agtgaattgt ggggccgcgt tgacccgaac
     5881 ttcccgtttg ccatgctgtt cccaggctcc cgtacagaag atattttgct gctgcaaacc
     5941 aacccgcagt ggcaatccat tttcgacact tacggtgtgc tgccgcgcca cccatcacag
     6001 ctttacgaac tgctgctgga aggtgtggtg ctgtttatta tcctcaacct gtatattcgt
     6061 aaaccgcgcc caatgggagc tgtctcaggc ttgttcctga ttggctacgg cgcgtttcgc
     6121 atcattgttg agtttttccg ccagcccgac gcgcagttta ccggtgcctg ggtgcagtac
     6181 atcagcatgg ggcaaattct ttccatcccg atgattgtcg cgggtgtgat catgatggtc
     6241 tgggcatatc gtcgcagccc acagcaacac gtttcctgag gaaccatgaa acagtattta
     6301 gaactgatgc aaaaagtgct cgacgaaggc acacagaaaa acgaccgtac cggaaccgga
     6361 acgctttcca tttttggtca tcagatgcgt tttaacctgc aggatggatt cccgctggtg
     6421 acaaccaaac gttgccacct gcgttccatc atccacgaac tgctgtggtt cctgcagggc
     6481 gatactaaca ttgcttatct acacgaaaac aatgtcacca tctgggacga atgggccgat
     6541 gaaaatggcg acctcgggcc agtgtatggt aaacagtggc gcgcctggcc aacgccagat
     6601 ggtcgtcata ttgaccagat tactacggta ctgaaccagc tgaaaaacga cccggattcg
     6661 cgccgcatta ttgtttcagc gtggaacgta ggcgaactgg ataaaatggc gctggcaccg
     6721 tgccatgcat tcttccagtt ctatgtggca gacggcaaac tctcttgcca gctttatcag
     6781 cgctcctgtg acgtcttcct cggcctgccg ttcaacattg ccagctacgc gttactggtg
     6841 catatgatgg cgcagcagtg cgatctggaa gtgggtgatt ttgtctggac cggtggcgat
     6901 acgcacctgt acagcaacca tatggatcaa actcatctgc aattaagccg cgaaccgcgt
     6961 ccgctgccga agttgattat caaacgtaaa cccgaatcca tcttcgacta ccgtttcgaa
     7021 gactttgaga ttgaaggcta cgatccacat ccgggcatta aagcgccggt ggctatctaa
     7081 ttacgaaaca tactgccaga gccgacgcca gtgtgcgtcg gtttttttac tctccgttaa
     7141 attcttcgag acgccttccc gaaattttgc aacgtcctgc aacggcgtaa atagtccgga
     7201 agatgcgccg aagaaataga aactggccct cgtctttttt tctcctcgct ccatactgcc
     7261 ggcatgaaaa cacaacgtgg ttatacgctg attgaaacgc tggtcgcgat gctgattttg
     7321 gtcatgctaa gcgcaagtgg actctatggc tggcaatact ggcagcagtc gcaacggctt
     7381 tggcaaaccg ccagctaggc gcgggactat ttgctctatt tacgtgaaga tgccaactgg
     7441 cataaccgcg accacagtat cagcgttatc agggagggga cgttatggtg ccttgtgagt
     7501 tccgctgctg gggccaatac ctgtcatggc agttcaccat tggtctttgt gccacgctgg
     7561 cccgaagtcg aaatgagcga cctgacacct tcgcttgctt tctttggcct gcgcaatacc
     7621 gcatgggccg ggcatattcg cttcaaaaac tcaacgggcg agtggtggct ggtggtttcg
     7681 ccgtggggaa gactccggct ttatcagcaa ggagaaacag aaggatgcct gtaaaagagc
     7741 aaggtttttc tctgctggaa gtgttgattg ctatggcgat cagtagcgta ttgttgctgg
     7801 gggctgcacg ctttctgcct gcgttacagc gtgaaagttt aacgagcact cgtaagctgg
     7861 cactggaaga tgaaatctgg ctgcgggtat ttaccgtcgc gaagcatctc cagagggcgg
     7921 gttattgtca tggcagctgt accggcgaag ggctggaaat tgtcggacag ggtgactgta
     7981 tcattgtgca gtgggatgcg aacagtaacg gtatctggga tcgcgaaccg gtaaaagagt
     8041 ccgaccagat tggatttcgt ctgaaggagc atgtgctgga aacgctacgc ggtgcgacat
     8101 cctgtgaagg taagggctgg gataaagtca ctaatccgga tgccatcatt atcgacactt
     8161 ttcaggtcgt acgtcaggat gtcagcggct tctcgccggt gttgacggtt aatatgcgtg
     8221 ctgccagtaa gtctgaaccg caaaccgtgg tggatgccag ctatagcgtg acaggattca
     8281 acctgtgaac cgcgaaaaag gtgtttcgtc acaggctctg gtcctgatgc tgctggtttt
     8341 gggtagcttg ctattgcaag gaatgagtca gcaggatcgc agttttgcct ctcgcgtgag
     8401 catggaaagt cagtcattgc gccgccaggc catcgttcag tcggcgctgg cgtggggaaa
     8461 aatgcactcc tggcagacgc agccagcagt tcagtgctcg cagtacgctg gaaccgatgc
     8521 ccaggtttgt ttgcgtttac tggcagataa tgaagcctta ttgattgccg gttatgaagg
     8581 cgtttcgttg tggcgaacag gcgaagtcat tgatggaaaa attgtttttt cgccacgcgg
     8641 ctggagcgat ttttgtccgc tgaaagaggg ggcattatgt cagcttccct gaagaatcaa
     8701 caaggcttta gcctgccgga ggtaatggtt gcgatggtgc tgatggtgat gattgtcact
     8761 gcgttatcgg gtatccagcg aacattaatg aacagtctcg ccagcagaaa ccagtaccaa
     8821 cagctctggc ggcatggttg gcagcaaacg caactgcgcg cgatttcgcc acctgccaac
     8881 tggcaggtca accgaatgca gacatcgcag gcgggatgtg tcagcatcag cgttacgcta
     8941 gtttcacccg ggggcagaga aggcgagatg acccgcctgc attgcccgaa tcgtcagtag
     9001 tcaggagccg ctatgttaag ggtctaccat tccaatcgtc tggacgtgct ggaagcgttg
     9061 atggagttta ttgtcgaacg cgaacggctg gacgatcctt tcgaaccaga gatgattctg
     9121 gtgcaaagta ccggtatggc gcagtggctg caaatgaccc tgtcgcaaaa gtttggtatt
     9181 gcggcaaaca ttgattttcc gctgccagcg agctttatct gggatatgtt cgtccgggtg
     9241 ttaccggaaa tccccaaaga gagcgccttt aacaaacaga gcatgagctg gaaactgatg
     9301 actctgctgc cgcaattgct ggagcgcgaa gactttaccc tgttgcggca ttatctgact
     9361 gacgatagcg acaagcgaaa actgttccag ctttcctcaa aagcggcgga cctgtttgac
     9421 cagtatctgg tctatcgtcc ggactggctg gcacagtggg aaacaggaca tttggttgaa
     9481 gggctgggag aagcacaggc ctggcaagcc ccgttgtgga aggcgctggt ggaatatacc
     9541 catgaactcg gacaaccgcg ctggcaccgc gcgaatctct atcagcgctt tatcgaaacg
     9601 ctggagtccg cgacgacctg cccgccgggg ttaccttcgc gcgtctttat atgcggtatt
     9661 tccgcgttac cgcctgttta tctccaggcg ctccaggcgc tgggtaaaca tattgaaatc
     9721 catctcctgt ttaccaaccc ctgccgttat tactggggcg atattaaaga tcctgcttat
     9781 ctggcgaaac tgctgactcg tcagcgtcga cacagttttg aagatcgcga attgccgcta
     9841 tttcgcgata gcgaaaatgc cggacagctc tttaacagcg atggtgaaca ggatgtcggc
     9901 aacccgctgc tggcctcatg ggggaagctt gggcgcgact acatttatct cctttctgac
     9961 ctggagagca gccaggagct ggacgctttt gtcgatgtga cgccagataa cctgctgcat
    10021 aacattcagt ctgacattct ggaactggaa aaccgcgccg ttgcgggtgt gaacatcgaa
    10081 gagttttccc gtagcgataa caaacgcccg cttgatccac tggatagcag tatcacattc
    10141 cacgtttgcc atagcccgca gcgtgaagtt gaagttttac acgatcgctt gctggcgatg
    10201 ctggaggaag ccccgacact tactccgcgc gacatcatcg tgatggtggc tgatatcgac
    10261 agctacagtc cgtttattca ggcagtgttt ggtagcgcac ctgcggatcg ttacctgcct
    10321 tacgccattt ccgaccgtcg tgcgcggcag tcacatccgg tactggaagc gtttatcagc
    10381 ctgttatcac tgcctgacag tcgctttgtg tcagaggatg tgctggcgct gctggatgtg
    10441 ccggtgctgg cggcgcggtt tgacatcacc gaagaagggc tgcgttattt acgccagtgg
    10501 gtcaacgaat ccggcattcg ttgggggata gatgacgaca acgttcgcga gctggaactc
    10561 cccgccaccg gacaacacac ctggcgattt ggcctgacgc gtatgttgct gggctacgcg
    10621 atggagagcg cgcagggcga gtggcaatcg gttctacctt atgatgaatc gagcggctta
    10681 attgcagaac tggtggggca tctggcttca ctgctaatgc agctaaacat ctggcgtcgc
    10741 gggctggcac aggagcgtcc gctagaagag tggttgccgg tttgtcgcga tatgctcaac
    10801 gccttcttcc tgccggatgc ggaaaccgaa gcggcgatga cgctgatcga acaacaatgg
    10861 caggcgatta tcgccgaagg tttaggtgcg cagtatggcg acgcggtgcc gctgtcacta
    10921 ttgcgtgatg aactggcaca gcgtctggat caagaacgta tcagccagcg ttttctcgcc
    10981 ggaccggtta atatttgtac tctgatgcca atgcgttcaa ttccgttcaa agtggtttgc
    11041 ctgctgggaa tgaacgacgg cgtttatcca cgtcagcttg cgccattggg ctttgacctg
    11101 atgagccaga aaccgaagcg tggcgaccgt agccgtcgcg atgacgaccg ctacctgttc
    11161 ctggaagcgt taatttccgc gcagcaaaaa ctctatatca gctatatcgg gcgttccatt
    11221 caggataaca gtgaacgttt cccgtcggta ctggtgcagg aactgatcga ctacatcggg
    11281 caaagtcatt atctaccggg cgatgaagcg ctcaactgtg atgaaagcga ggcaagggta
    11341 aaagcgcatc ttacttgcct ccatacccgg atgccgtttg atccgcaaaa ctaccagcca
    11401 ggcgaacgac aaagctatgc tcgtgaatgg ctacctgcgg ccagccaggc tggtaaagca
    11461 cattctgaat ttgttcagcc gctgccgttt accttaccgg aaaccgtgcc gctggaaacg
    11521 ctacaacgat tctgggcaca tccggtgcgg gcgttcttcc agatgcgttt gcaggtgaac
    11581 ttccgtactg aagacagcga aatcccagac accgagccat ttattctgga aggacttagc
    11641 cgttatcaaa tcaatcagca gttattgaat gcactggttg agcaggatga tgccgaacgc
    11701 ttgttccgcc gcttccgggc ggcaggggat ttaccgtatg gcgcttttgg tgaaattttc
    11761 tgggaaacac agtgccagga gatgcagcag cttgccgaca gagtcattgc ctgtcgccag
    11821 ccggggcaga gtatggaaat tgatctcgcc tgcaacggtg tgcagataac tggctggttg
    11881 ccgcaggtgc agccggatgg cctgttgcgc tggcgtccct ctttattaag tgtggcgcag
    11941 ggaatgcaac tttggctgga acaccttgtc tactgtgcca gcggtggtaa tggcgaaagt
    12001 cgcctttttc tacgcaaaga cggtgagtgg cgttttccgc cgcttgcagc cgaacaggct
    12061 ttgcattacc tctcacaact gattgagggg tatcgtgaag gaatgtccgc gccattgctg
    12121 gtgttacctg aaagtggcgg cgcgtggcta aaaacctgtt atgacgcgca aaacgatgcc
    12181 atgctggatg acgattccac gttgcaaaaa gcccgtacga aattccttca ggcttacgaa
    12241 ggcaacatga tggtgcgtgg cgaaggtgat gatatctggt atcaacggct ctggcggcaa
    12301 ttaacaccag agacaatgga ggccatcgtt gaacagtcgc aacgtttcct gttaccgctg
    12361 tttcgcttta atcagtcatg agtgttgtat aaaaattgcg caatctatcc gcttacttta
    12421 tgatgcgcac cagtcacgga ctgatggtta tataaacata ggctgactcg tgcagcacaa
    12481 gattaaattc tggcagatga tttgcgttaa cctgttgaat ctgtccagaa aataaagttg
    12541 attatgaggt ccgtgaatgc cccgcagcac ctggttcaaa gcattattgt tgtttgttgc
    12601 cctctgggca ccattaagtc aggcagaaac gggatggcag ccgattcagg aaaccatccg
    12661 taaaagtgat aaagataacc gccagtatca ggctatacgt ctggataacg gtatggtggt
    12721 cttgctggtt tctgatccgc aggcagttaa atcgctctcg gcgctggtag tgcccgttgg
    12781 gtcgctggaa gatcccgagg cgtaccaggg gctggcacat taccttgaac atatgagtct
    12841 gatggggtcg aaaaagtacc cgcaggctga cagtctggcc gaatatctca aaatgcacgg
    12901 cggtagtcac aatgccagca ctgcgccgta tcgcacggct ttctatctgg aagttgagaa
    12961 cgacgccttg cccggtgcgg tagaccgcat ggccgatgct attgcagaac ccttgctcga
    13021 caagaaatac gccgaacgtg agcgtaatgc agtgaacgct gaattaacca tggcgcgtac
    13081 gcgtgacggg atgcgcatgg cacaggtcag cgcagaaacc attaacccgg cacaccccgg
    13141 ttcaaagttt tctggtggta acctcgaaac tttaagcgac aaacctggta atccggtgca
    13201 gcaggcgctg aaagatttcc acgagaagta ctattccgcc aatttgatga aggcggttat
    13261 ttacagtaat aaaccgctgc cggagttggc aaaaatggcg gcggacacct ttggtcgcgt
    13321 gccgaacaaa gagagcaaaa aaacggaaat caccgtgccg gtagtcaccg acgcgcaaaa
    13381 gggcattatc attcattacg tccctgcgct gccgcgtaaa gtgttgcgcg ttgagtttcg
    13441 catcgataac aactcagcga agttccgtag taaaaccgat gaattgatta cctatctgat
    13501 tggcaatcgc agcccaggta cactttctga ctggctgcaa aagcagggat tagttgaggg
    13561 cattagcgcc aactccgatc ctatcgtcaa cggcaacagc ggcgtattag cgatctctgc
    13621 gtctttaact gataaaggcc tggctaatcg cgatcaggtt gtggcggcaa tttttagcta
    13681 tctcaatctg ttacgtgaaa aaggcattga taaacaatac ttcgatgaac tggcgaatgt
    13741 gctggatatc gacttccgtt atccgtcgat cacccgtgat atggattacg tcgaatggct
    13801 ggcagatacc atgattcgcg ttcctgttga gcatacgctg gatgcagtca atattgccga
    13861 tcggtacgat gctaaagcag taaaggaacg tctggcgatg atgacgccgc agaatgcgcg
    13921 tatctggtat atcagcccga aagagccgca caacaaaacg gcttactttg tcgatgcgcc
    13981 gtatcaggtc gataaaatca gcgcacaaac tttcgccgac tggcagaaaa aagccgccga
    14041 cattgcgctc tctttgccag agcttaaccc ttatattcct gatgatttct cgttgattaa
    14101 gtcagagaag aaatacgacc atccagagct gattgttgat gagtcgaatc tgcgcgtggt
    14161 gtatgcgcca agccgttatt ttgccagcga gcccaaagct gatgtcagcc tgattttgcg
    14221 taatccgaaa gccatggaca gcgcccgcaa tcaggtgatg tttgcgctca atgattatct
    14281 cgcagggctg gcgcttgatc agttaagcaa ccaggcgtcg gttggtggca taagtttttc
    14341 caccaacgct aacaacggcc ttatggttaa tgctaatggt tacacccagc gtctgccgca
    14401 gctgttccag gcattgctcg aggggtactt tagctatacc gctacggaag agcagcttga
    14461 gcaggcgaag tcctggtata accagatgat ggattccgca gaaaagggta aagcgtttga
    14521 gcaggcgatt atgcccgcgc agatgctctc gcaagtgccg tacttctcgc gagatgaacg
    14581 gcgtaaaatt ttgccctcca ttacgttgaa agaggtgctg gcctatcgcg acgccttaaa
    14641 atcaggggct cgaccagagt ttatggttat cggcaacatg accgaggccc aggcaacaac
    14701 gctggcacgc gatgtgcaaa aacagttggg cgctgatggt tcagagtggt gtcgaaacaa
    14761 agatgtagtg gtcgataaaa aacaatccgt catctttgaa aaagccggta acagcaccga
    14821 ctccgcactg gcagcggtat ttgtaccgac tggctacgat gaatacacca gctcagccta
    14881 tagctctctg ttggggcaga tcgtacagcc gtggttctac aatcagttgc gtaccgaaga
    14941 acaattgggc tatgccgtgt ttgcgtttcc aatgagcgtg gggcgtcagt ggggcatggg
    15001 cttccttttg caaagcaatg ataaacagcc ttcattcttg tgggagcgtt acaaggcgtt
    15061 tttcccaacc gcagaggcaa aattgcgagc gatgaagcca gatgagtttg cgcaaatcca
    15121 gcaggcggta attacccaga tgctgcaggc accgcaaacg ctcggcgaag aagcatcgaa
    15181 gttaagtaaa gatttcgatc gcggcaatat gcgcttcgat tcgcgtgata aaatcgtggc
    15241 ccagataaaa ctgctgacgc cgcaaaaact tgctgatttc ttccatcagg cggtggtcga
    15301 gccgcaaggc atggctattc tgtcgcagat ttccggcagc cagaacggga aagccgaata
    15361 tgtgcatcct gaaggctgga aagtgtggga gaacgtcagc gcgttgcagc aaacaatgcc
    15421 cctgatgagt gaaaagaatg agtgatgtcg ccgagacact agatcctttg cgcttgccct
    15481 tacagggcga gcgcctgatt gaagcctctg ccggcacagg caaaaccttt acgattgcgg
    15541 cgctctattt gcgcctgtta cttggactag gcggttccgc cgcctttccc cgcccgctga
    15601 tcgttgaaga actgctggtg gtcaccttta ccgaggctgc cacggcagaa ttgcgcggtc
    15661 gtatccgtag caatatccac gagttgcgca tcgcctgtct gcgtgaaacc accgacaatc
    15721 cactgtacga acgcctgctg gaagagatcg acgataaagc gcaagccgcg cagtggttgt
    15781 tgttagccga acggcagatg gatgaagcgg cagtctttac tattcacggc ttttgccagc
    15841 gcatgctcaa cctgaatgcc tttgaatccg gcatgctgtt tgagcagcag ctgattgaag
    15901 atgagtctct gctacgctac caggcctgcg ccgatttctg gcgtcgccac tgctacccgc
    15961 tgccgcgtga aatagcccag gtcgtctttg aaacctggaa agggccgcag gcgttgctgc
    16021 gcgatattaa tcgttatctg caaggcgaag cgccggttat caaagcaccg ccgcccgatg
    16081 atgaaacgct ggcttcccgc cacgcgcaaa ttgtggcgcg tattgatacg gtaaaacagc
    16141 agtggcgcga cgcagtgggt gaactggatg cgctgatcga atcttctggt attgatcgac
    16201 gcaagtttaa ccgtagcaat caggctaaat ggatcgaaaa gatcagcgct tgggcagaag
    16261 aagagacaaa cagttatcag ttgccggagt cgctggaaaa attctcccag cgtttcttag
    16321 aagatcgcac gaaggccggg ggggaaaccc cgcgacatcc actgtttgag gtgatcgatc
    16381 aactgcttgc agaaccattg tcgatccgcg atctggtgat cacccgagca ttggctgaga
    16441 tccgcgaaac agtagcgcgt gaaaaacgcc gccgtggcga attgggtttt gatgacatgt
    16501 taagtcggct cgattccgcg ctgcgtagcg aaagcggcga ggtgttggca gcggcgatcc
    16561 gtacgcgatt cccggtggca atgatcgatg aatttcagga taccgacccc cagcagtacc
    16621 gaatttttcg ccgtatctgg caccatcagc cggaaaccgc attgttgctg attggcgacc
    16681 cgaagcaggc catatatgca ttccggggtg cggatatctt cacttatatg aaggcgcgta
    16741 gtgaagttca cgcccactac actttagaca ccaactggcg ctccgcacct ggaatggtga
    16801 acagtgtgaa caagcttttc agccagactg atgacgcgtt catgtttcgc gaaataccgt
    16861 ttattccagt gaaatcagcc gggaaaaatc aggcgttacg ttttgtattt aaaggtgaaa
    16921 cgcagcctgc gatgaaaatg tggctgatgg aaggcgaaag ctgcggcgtt ggcgactatc
    16981 aaagtaccat ggcgcaggta tgtgctgcgc aaatccgcga ctggctacaa gccggacagc
    17041 ggggcgaagc gttgctgatg aacgacgacg acgcgcgtcc ggtgcgtgct tcggacatca
    17101 gcgtgctggt gcgcagccgc caggaggccg cccaggtgcg cgatgcctta acgttgctgg
    17161 aaatcccttc cgtttacctt tcgaaccgcg acagtgtttt tgaaactctg gaagcgcagg
    17221 aaatgctttg gttgttgcag gcggtgatga cgcccgaacg tgagaacacc ctgcgcagtg
    17281 cgctggcaac gtcaatgatg gggctgaatg cgctggatat tgaaacgctg aataatgacg
    17341 aacatgcgtg ggatgcggta gtcgaagagt tcgatggtta tcggcaaatc tggcgcaaac
    17401 gtggcgttat gccgatgctg cgggcgctga tgtcggcgcg taacattgca gaaaacttgc
    17461 tggcaacggc aggcggtgag cggcgtctta ccgatatctt gcatatcagc gaactgcttc
    17521 aagaagccgg aacgcagctg gaaagtgaac atgcgctggt acgctggtta tcgcaacata
    17581 tcctcgagcc agacagtaat gcctccagcc aacaaatgcg cctcgaaagt gataaacatc
    17641 tggtgcagat tgtcacgatc cataaatcga aagggctgga atatccgctg gtctggttgc
    17701 catttattac caatttccgc gtccaggatc aggcgtttta tcacgatcgc cactcgtttg
    17761 aggcagtttt ggatcttaat gctgcgccag aaagcgtcga cctcgcggag gtcgaacgtc
    17821 tggcggaaga tctgcgtttg ctttacgtgg cgctgacgcg ttcggtttgg cattgcagtc
    17881 tcggcgttgc accgctggtg cgccgtcgtg gcgataaaaa aggtgacacc gacgtccacc
    17941 aaagtgcgct cgggcgtttg ctgcaaaaag gggaaccgca agatgcggca gggcttcgca
    18001 cctgtattga agcgttatgc gatgatgata ttgcctggca aacggcacaa actggtgata
    18061 accagccctg gcaggttaat gatgctttaa ctgcagaact gaatgcgagg acactacaac
    18121 gattgcccgg cgataactgg cgtgtcacca gctactccgg cttgcagcag cgtggtcacg
    18181 gtatcgctca ggatctgatg ccacggctgg atgttgatgc cgcaggcgtg gtcagcgtcg
    18241 ttgaagaacc gacgttaaca ccgcatcagt tcccgcgcgg tgcgtcaccg gggacattct
    18301 tgcacagttt gtttgaagac ctcgatttta cccagccggt tgacccgaac tgggtacagg
    18361 aaaaactgga actcggcggc tttgaatcgc agtgggaacc ggtgttgacc gagtggatca
    18421 cggctgtcct ccaggcacct ctcaatgaaa cgggtgttag cctgagtcag ctttccgacc
    18481 gcgataaaca ggtggagatg gagttttacc tgccgattag cgaaccgctc atcgccagcc
    18541 agcttgatgc actaattcgc cagtttgacc cgctatccgc tggctgcccg ccgctggagt
    18601 tcatgcaggt acgcggcatg ttaaaaggct ttatcgacct ggtattccgc cacgaagggc
    18661 gttattacct gctcgactat aaatccaact ggctgggtga agacagttcg gcttacaccc
    18721 aacaggctat ggcagcagcg atgcaggcac accgctatga tctgcaatat cagctttata
    18781 ccctggcact gcatcgttat ctgcgccatc gcattgctga ttacgactat gatcttcact
    18841 ttggcggcgt tatctatctg ttcctgcgtg gcgttgataa agaacatccg caacaaggga
    18901 tttacacaac ccgacccaac gccgggttga ttgccctgat ggatgagatg tttgccggta
    18961 tgaccctgga ggaggcgtaa tgaaattgca aaagcaatta ctggaagctg tggagcacaa
    19021 acagctacgc ccgctggatg tgcaatttgc cctgaccgtg gcgggagatg aacatcctgc
    19081 tgtcaccctc gcggcggcac tgttaagtca tgatgccgga gagggacacg tctgtttgcc
    19141 gctttcacga ctggaaaata acgaggcgtc gcatccgctg ttggcgacct gtgtcagtga
    19201 aatcggtgag ctacaaaatt gggaagaatg cttgctggct tctcaagcgg tcagcagggg
    19261 agatgaaccc acgccgatga tcctctgtgg cgatcgtctt tatttgaatc gcatgtggtg
    19321 taacgagcgg acggtagcgc gcttcttcaa tgaggtgaat cataccattg aggttgatga
    19381 agctctactg gcgcaaaccc tggacaaact ttttccagta agcgatgaaa ttaactggca
    19441 aaaagttgca gcggcagtgg cgctgacgcg gcggatctcg gtgatttccg gcggcccagg
    19501 caccggtaaa acgaccaccg tggcgaagtt gctggcagcg ttaattcaaa tggccgacgg
    19561 cgaacgctgc cgtatccgtc tggctgcacc aacgggtaaa gctgccgcgc gcttaaccga
    19621 atctctcggc aaggctttgc gacagttacc gctgaccgat gaacaaaaga aacgcattcc
    19681 ggaagatgcc agcactttgc accgattgct gggcgcgcag ccgggtagcc agcgtttacg
    19741 tcatcatgcc ggtaacccgc tgcatcttga tgtgctggtg gtagatgaag cgtcaatgat
    19801 cgatctgcct atgatgtcga gactgatcga cgccttgcca gatcatgcgc gagtgatctt
    19861 tctcggcgat cgtgatcaac tggcctcggt tgaggctggg gctgtgctgg gcgatatctg
    19921 cgcttatgcc aacgcgggct ttaccgccga gcgtgccagg cagctaagcc gcctgacggg
    19981 gactcacgtt ccggcaggaa ccggcacaga agcggcatct ttgcgcgaca gtctctgcct
    20041 gctgcaaaaa agctatcgtt tcggcagcga ttctggcatt ggtcagttag ctgcggcgat
    20101 caaccgtggt gataaaacgg cagtgaaaac cgtttttcag caggatttta ctgatatcga
    20161 aaaacgactt ttacagagcg gcgaagatta tattgcgatg cttgaggaag ctcttgcggg
    20221 ttacggacgt tatctggatc tgctgcaagc gcgtgccgag ccggatttaa tcattcaggc
    20281 gttcaatgag taccagcttt tgtgcgccct gcgggaaggg ccgtttggcg tggctggact
    20341 gaatgagcga attgagcagt ttatgcaaca gaagcgcaaa attcatcgtc atccgcactc
    20401 tcgttggtac gaaggccgac cggtgatgat tgcccgtaat gacagcgcgc ttgggttgtt
    20461 taatggcgat atcggtattg cgctggatcg cgggcagggg acgcgcgtct ggtttgcgat
    20521 gccggacggc aatattaagt ctgtgcaacc gagtcgcttg ccagagcacg aaactacgtg
    20581 ggcgatgacg gtacataaat cgcagggatc ggagttcgac catgcggcgt tgattttacc
    20641 gagccaacgc acgccggtag taacgcgaga gctggtatac accgcggtga cccgcgcgcg
    20701 tcgtcgtctg tcgctgtatg ccgatgagcg tatattaagt gcggcaatcg ccactcgtac
    20761 tgagcggcgc agtggtctgg cggcgttgtt tagttcgcgg gaataaacgt aattgccgga
    20821 tgcgacgcac gagtgttacg cctgccgcat ccggtgattt tcatcgctta ccctaaatcc
    20881 gccatcaaca ctttggattt acgctggtag ttgtacaact gctttttgct ctcgggcagt
    20941 aaatcaatat ccaccggggt aaatccacgt tcctggaacc agtgaatact gcgcgtggtc
    21001 agcacaaaca atttgcttaa gccgctctgc ttcgcctgag cggcaatgcg ttccagcaga
    21061 acctcgcccc gtgatgaact gcggtaatcc gggtgaactg ccacacaggc catttcccca
    21121 atcttctctt ccgggaacgg atagagcgcg gcgcaggcaa tagtcgtgtt atcgcgctga
    21181 ataatggtga atttgtcgat ttccatctcc agctgctcgc gagaacggcg caccagaata
    21241 ccttgctgct ccagcgggcg aatcaactcc agaataccgc caatatcgtt gattgttgcg
    21301 cgacgaatct gctcggcgct ttccatcaca atctgcgtac cgataccgtc gcgtgagaac
    21361 aactcttgca acagcgcgcc atcttcctga taactgatta aatgacagcg gcgcacgccg
    21421 ctgcggcagg ctttcactgc gccacgcaaa aagcgcaccg taccggagtt gtaatcgcct
    21481 ttctcttcct gggcttctac ccgtgcttgc gcttcgttag ggaaaagttc ggagacaata
    21541 tcaccgtcgt cattagtaac gccctgggaa gagcaaaaac caatcatctt ttcagctttc
    21601 agtttgatgg ctagttgagt ggcaatctct tcagaggtca gattaaagct ctcgccagtg
    21661 actgaaacag cgaccggccc cattagcact attgcaccgc tttccagttg acgatggatc
    21721 gcgtcttcat caatccgccg gatacgcccg ctatggcagt aatccacgcc gtcatcgacg
    21781 cccagcggct gggcaataat aaaattgcca ctaacgacgt tgatatgcgc gccctgcagc
    21841 ggcgtgttat tgagactcat tgacaggcga gcagtaatat ccagttgcaa tgttcccgca
    21901 gcctgcttca ccagttccag tgttttggcg tcggtcacac gtatattctt gtgatacagc
    21961 ggttcgtggt gatgcgcagc cagatttgcg tcgatctgcg gacgtgcgcc atagaccacc
    22021 accagacgga tgccgaggct gtgcaacaac ccgatatcat taacgatact ggagaaattc
    22081 tcatgctcaa tggcttcacc gccgagcatg atgacaaacg tttttccccg gtgggtattg
    22141 atatagggaa ccgaatggcg gaatccctcg accagctcgg ttttacgttc ctttaccacg
    22201 gcacacccct ttgcatgatt atttgaaatt attgtatttt tattctgttt tttcgcaggg
    22261 tgcaagtgta aattttatgc gagagcgact tttttatcag taatgccgtg aatataaaaa
    22321 gaatgtttac cgtttattag atgacagatt atgcgtcttt cgctaaagtt tccggtcaaa
    22381 ttagtcgttt acttgttaca cagcttagat ttcttttgct cgggagaggc atgtcaggat
    22441 ccaacactgc aatcagccgt cgtcgtttac tgcaaggcgc gggcgctatg tggctattga
    22501 gcgtaagtca ggtcagcctg gctgcggtta gccaggtcgt ggcggtgcgc gtctggcctg
    22561 cgtccagcta cacccgcgtg acggtagaat caaatcgtca gctgaaatat aagcagttcg
    22621 cgttgagtaa ccctgaacgc gtggtggtgg atatcgaaga tgtaaacctg aactcggtac
    22681 tcaaggggat ggcggcacaa attcgtgcag acgacccgtt catcaagtcg gcgcgcgtcg
    22741 ggcaatttga cccgcaaacc gtacgcatgg tttttgaatt aaagcaaaac gtaaaaccgc
    22801 agctgtttgc ccttgcgccg gtcgccgggt ttaaagagcg tctggtgatg gacctctatc
    22861 cggccaatgc acaggatatg caggacccgc tgctggcgct gctggaggat tacaacaaag
    22921 gcgacctcga aaagcaggtg ccgccagcgc aaagtggtcc acaaccgggt aaagcagggc
    22981 gcgatcgtcc gattgtcatt atgcttgacc ctggccacgg tggcgaagat tccggtgcgg
    23041 tggggaaata caaaacgcgc gaaaaagatg tggtattgca aatagctcgc cgtttgcgct
    23101 ctctgatcga gaaagagggc aatatgaagg tgtacatgac gcgcaatgaa gacatcttca
    23161 ttccgttgca agtgcgcgta gcaaaagccc agaaacagcg tgctgacctg tttgtctcta
    23221 tccatgccga cgcctttacc agtcgtcagc cgagcggttc ctcggtgttt gcgctctcaa
    23281 ccaaaggcgc aaccagtact gcggcaaaat atctggcaca aacccagaac gcctcggact
    23341 tgattggtgg cgtgagcaaa agcggtgacc gctatgtcga ccacaccatg ttcgatatgg
    23401 tgcagtcgct gactattgct gacagcctta agtttggtaa agcggtactg aataagctcg
    23461 gaaaaatcaa caagctgcat aaaaatcaag ttgaacaggc cgggtttgcc gtactaaagg
    23521 caccagatat tccctccatt ctggtcgaaa cggcgtttat cagtaacgtt gaggaagagc
    23581 gtaaactgaa aacggcgact ttccagcagg aagttgcgga gtctattctt gcggggatta
    23641 aagcgtattt tgccgatggg gcaacgctgg cgagaagggg atgataaaaa ggcgctgaat
    23701 ggcgcttttt ttatgggaga tcagaaacaa aaaaacaccc gttagggtgt tcaataattg
    23761 gttgcggggg ccggatttga accgacgacc ttcgggttat gagcccgacg agctaccagg
    23821 ctgctccacc ccgcgtccgt ggatgcgcac tatactccgc tcgcgttttc atgcaacctt
    23881 ttttttaccc cgatcacgga tttagcatga ttttgaacaa aaacaaacca ctaaagctta
    23941 ttttttgtgc gaaatttgcg tcagggagag gctgctcatt tcattaaacg cggcggtttg
    24001 ttatcttcgt tgcgccttat tttttaacct gaagaagaga acaatgaaag gacgttgggt
    24061 aaagtacctt cttatgggca cggttgtggc aatgcttgcc gcctgctctt ccaaaccaac
    24121 cgatcgcgga cagcaatata aagacgggaa atttacccag cctttctctc tggtgaacca
    24181 gccagatgcc gttggcgcgc cgattaacgc cggtgatttt gccgagcaaa ttaaccatat
    24241 ccgtaattcg tcaccgcgtc tgtatggcaa ccagagtaat gtttataacg cggtgcaaga
    24301 gtggctgcgc gcaggcggtg atacccgcaa tatgcgccag ttcggcattg atgcctggca
    24361 gatggaaggt gccgacaact atggtaacgt gcagtttaca ggctattaca cgccggtaat
    24421 tcaggcgcgc catacccgcc agggcgagtt ccagtatcct atttaccgta tgccgccaaa
    24481 acgtggtcgt ctgccgtctc gtgcggagat ctacgccggg gcattgggcg ataaatacat
    24541 cctcgcttac agcaactctc tgatggataa cttcattatg gatgtgcagg gtagtgggta
    24601 tatcgacttt ggtgatggca gtccgcttaa ctttttcagc tatgcaggga aaaacggtca
    24661 tgcctatcgc agcattggta aggtgctgat cgaccgtggc gaagtgaaaa aagaagatat
    24721 gtcgatgcag gcgattcgtc attggggcaa aacacacagt gaagccgagg ttcgcgagct
    24781 gctggaacag aacccgtctt tcgtcttctt taaaccgcaa tcttttgcac cggtgaaagg
    24841 ggcaagtgcg gtgccgctgg ttggccgtgc ttctgttgcc tccgatcgtt caattattcc
    24901 gccaggtact accttgctgg cagaagtgcc gctgctggat aataacggca aatttaacgg
    24961 tcagtacgaa ttgcgtctga tggtggcgtt ggatgtcggg ggcgcaatca aaggccaaca
    25021 cttcgatatc tatcaaggga tcgggccgga agccggacac cgcgcaggtt ggtacaacca
    25081 ctatggacgg gtttgggtgc tgaaaacggc tccgggcgca ggtaacgtct ttagcggctg
    25141 atgtgatatt ctgtatgccg tttttacgga tatgacaggg tgaggggtaa cctcacatcc
    25201 tgattattga ctaagtgcgc gttgttcata ccggatgcgg cgtgaacgcc ttatccggcc
    25261 tacaaatcat gcaaatccaa aatattgcag gagttatgta ggcctgataa gcgtagcgca
    25321 tcaggcattt atgcgtttag ggtgagggat aacctcgccc tttttaattc tgaggtgcta
    25381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
    25441 gtgaaaaagc gttgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
    25501 tcggttcctg ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc acgcttatcg
    25561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
    25621 tcgggctggc aaaagcggaa gtcatggcgg agcgcattcg ccagattaac ccggagtgcc
    25681 gtgtaacggt ggtggatgat ttcgtgacgc cggataacgt agcgcaatat atgagtgccg
    25741 gttattcgta cgtgattgat gccattgata gtgtacggcc caaagcggcg ctgattgctt
    25801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc
    25861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggatccgctg gcggcgaagt
    25921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
    25981 tggattgcgt gttttctact gaagcgctgg tgtacccgca gtcagacggt acggtgtgtg
    26041 cgatgaaagc cacggcagaa gggccgaagc ggatggattg tgcatctgga tttggcgcgg
    26101 caacgatggt gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg aagaagatga
    26161 tggcgaaagc ggcgcgtcag ggttaaacaa tcaggtcgga tgagacgcga acgtcttatc
    26221 cgacccggtt ctgtgcttaa acctgcttcg tcgcagcgat aatcgcctcg cttaacgcat
    26281 ttaacccctg gctgcgtgag gcgctaagct gcgcacgtaa tcccagctca tcaaacaatg
    26341 ccagcggcga ctgtgcctgt aattcggcgg cggttttccc ctcaacggca gtcaacaaca
    26401 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaagtgc attttgccgt
    26461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
    26521 gcgcttttaa ctcgtctggc aaagccggaa gctgtttccc cagcatgatc aactggcgat
    26581 atttatcttc ccattgcgac aacggtgcga aggtattgcg taacgtttct gcggttacgg
    26641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
    26701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
    26761 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgttg
    26821 cccggcccgc aaggcaatac cgtactccgc cagtagcgtc accatatcgc tgtgatgaac
    26881 gccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
    26941 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcctg
    27001 gttgatatcg taatctgcca gccattccag cgccgcgctt aatcctatga cgccagcgac
    27061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtggtgaaac cgtcaaaact
    27121 cacttcgtga atcattttgc cgccacccag ccagggcgac atcgcttcca gcagttctga
    27181 tttgccatac agcacgccga tacccgtcgg gccatacagt ttgtgaccag agaaagcata
    27241 gaaatcgata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
    27301 aaccatcacc accatcccgg ctgaatgagc aaaggtaatc gctcgcgcca gatccgggca
    27361 accgccagtc acgttcgaca tctgacccaa cgccagaatc cgactacggg gagtaatcag
    27421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
    27481 ggctctagtt tgttgggcga ccatcagcca ggggacgagg ttagcgtggt gttctgccac
    27541 gctgacaata atctcatcgc ccggttgcag acgcggacgc gcatagcatt gtgccaccat
    27601 gttgatggat tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg gtgcattcag
    27661 taattgcgcc actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt gggcttcggc
    27721 aaactggctg cgatggacgt ttccggcgct caggctgtaa aactgtcgag tggcttcaac
    27781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
    27841 tgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
    27901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
    27961 tatctagata tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt taggcgttaa
    28021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
    28081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
    28141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
    28201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
    28261 tcaaccgtac tgacgtgaaa gagatggtcg aactggaaca gtaattcgcg atcgcaaggt
    28321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
    28381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
    28441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaatacg cattccgata
    28501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
    28561 acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt gtgatctaat
    28621 tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa
    28681 tgccttacga gtttttgatg ccgcagcacg ccatttaagt tttactcgcg cagcagaaga
    28741 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttttggg
    28801 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
    28861 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
    28921 ccgtagcgcc aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
    28981 ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc
    29041 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
    29101 gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat tgccggtgtg
    29161 ttcgccgcta ctgctgacag gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca
    29221 tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt
    29281 aaatcatatc aacgttcagc aagggccaat ttttagtcat agcgccatgg tgctgcaagc
    29341 ggctattcac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga
    29401 ggccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaatg ctttttatct
    29461 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
    29521 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
    29581 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
    29641 tgtggcactg ggcgcttttg gtgcgcatgt gttaagtaaa accatggggg ccgttgagat
    29701 gggctggatc caaaccggtc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
    29761 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
    29821 gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
    29881 gtgggcgttt gtcacaccgg ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt
    29941 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
    30001 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
    30061 aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat gaatgttatc
    30121 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
    30181 gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
    30241 ccattgtcgg catgttacag ggtgtagtag agaagggcgg tgaactgcgt gttgaagttg
    30301 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taagttcact gttccgctac
    30361 gcgctgcctt gcgcgatgct ggggtgctgg cgaactatga aacgccgaag cgtccggttg
    30421 tgcatgtatt ctttattgca ccaggatgct gctataccgg ttactcatac agcaacaata
    30481 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagaggcg ccgagtcgtt
    30541 ccacgctcaa actggaagag gcatttcatg tgtttattcc tgcggatgag tgggatgaac
    30601 gcctggcgaa cgggatgtgg gcggtggatt taggcgcttg ccctggcggc tggacctacc
    30661 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
    30721 tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc cgtccgacac
    30781 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggctaaa gttgcggcat
    30841 tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaat ctcaaactgc
    30901 cgatgaaaaa acgctacgaa gaagtatcac acaatctggc gtatattcag gcacagcttg
    30961 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
    31021 tgacggtgca cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
    31081 aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga
    31141 agttgcaacc tggcgctcat cagaaataat gtgcgttacc tcatccagat acccgatgcg
    31201 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
    31261 ttttaatagc atcgatttgt aatcagcgtt gatcgcattg gagtcccaca gtgcgccgct
    31321 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
    31381 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
    31441 cagttgaatg cgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac
    31501 ctgaatgttg atgtcaggca actggcgtgc cagataccag caagttgaac tggcgtctaa
    31561 ggctatcacc atcctttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
    31621 cgcataatgg cttttcagcc tgatgtgaaa gagagcgcta ctgtcttgat tttgacggtg
    31681 gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta attcattgag
    31741 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
    31801 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
    31861 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
    31921 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
    31981 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
    32041 cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat cgagagtgtc
    32101 accctctacc gccgccatca tcaccagcgg cggggcataa ctgtccagct caaataacgg
    32161 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
    32221 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gtcccatctc
    32281 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
    32341 cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
    32401 gtgcacgggc ttcttccggg ctgttaaatt cccctacgcc ataccaaccg aacagcgcag
    32461 ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg
    32521 ctttaatctg attccacaat gtgttgcgac ttcctccacc gactaacaat aattcatagg
    32581 ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gcagttaacc
    32641 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact ccttgccagc
    32701 cagcgttctg acacgacaat aaatcacact gcattttcac accatccgcg ccaggtgcga
    32761 tcagacgagc ttcttcaatc aacatttgcc agggtgtttc agccgtccag aacagttttc
    32821 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aaccatgcct
    32881 ggctatctag ttcgcaggtg gaaccggcgt actgacttaa cagcgaagta tcaacctagg
    32941 cgctgcgaac cattaaaatt tcccatgtac cggaggagag cacgggttca ttttgttcgg
    33001 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
    33061 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
    33121 ccaccagacg agggaagagt cggcgtggaa taccggtggc ttgtaaaatt tgcggactga
    33181 aatcgcgttg ctggatatcc agcatctggc tggttccggc tatcgtgata tcagtagtga
    33241 attcgccggt taaacggtgg ttaatcagcg acgaaatgaa gagccaggcg tgcgcgcgtt
    33301 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
    33361 aggctccgac gccagaaata gcctgcaacc gctgtgcgga gattaaccgt tcaatattgt
    33421 ccataaccgc tgctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
    33481 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg cggatgtggc
    33541 attcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcag
    33601 tctaaagacc actggtgcca ggtgttgttt tccatcgcga tatcgctggc attaggcgtt
    33661 gaggcgcggg caacaatttt tccctgccga ttaaccgcga tggccctgac attggtcgcg
    33721 ccacagtcga gtaccaggat aacttcttgt ttcataatcg ctcctgcaat atagccggat
    33781 aacattgttt atccggctaa ccactcttgc ttgttcaggc ccggtagccg gagcgaccgg
    33841 gcatcacatc agggtgtaat gtattaacgc ttgtacaacg gaccgtagtt ctggcaagcg
    33901 cggtaatcct ggccttcaat atccatgccg tgcgcagccc aggcagaagg acgatacact
    33961 ttggtctctt caacgttgtg catacatacc gggatacgca gcatggaggc aagggtgata
    34021 aagtctgcgc caacgtggcc gatggtcaga actccgtggt tggcacccca gttcgccatt
    34081 accgaataca catccgtaaa cggcccttta ccagtgaggc gcggtgcaaa ccaggtggtt
    34141 ggccaggttg agttggtgcg tttgttgagg atgtcatgca catccttcgg caattccacg
    34201 ctccagcctt ccgcgatttg cagtaccggt cccaggcctt tgatgatgtt gacacgagtc
    34261 atggtgaacg ggacgccgcc ttcggtaagg aagcgggaag agtaaccgcc gccacggaag
    34321 tattcgtgga tcgccgggca ccattcggta gcggcgaggc aagcgtcagc ctcttgctga
    34381 gagatttccc agtgcggctt catcgtcggg ttaccttcgc tgtcgcgttg tttacaggaa
    34441 ccgtccagcg cagcagaacc tgagttgatc aaatggatga tgccgtgttc tgccagtccg
    34501 tccagtttat gccccgttac acgctcaatt gcttctggtg accagtaggt acgcacatcg
    34561 gcaaatacct gagcggtgcc ggtgagctgg tgacccatta gcattgccac gccgttaaga
    34621 ctgtcgtttt cggtcgccac gacaaagggt tcgcgcacgc cattccagtc aaatgaactg
    34681 ttgaggatcg cttcggcggt gtcaccattg ggatattgat cggtccagtg acgttgcccc
    34741 tggaagcccg cagcgatggc gttgtagcca agtgattctt ccacgcgacc aatatcggcc
    34801 agtttgctgt tgccttgcat catgtcgcgg atacacatcg ccatcagtaa actttcgcgc
    34861 agaactgcgc ggctttgctc ggcattgcgt tgatactgtt tgttattttc atcttcgcca
    34921 tagcggaagt ttttatcagc ccaggccagt gccatttcca gttcggcttc gtcgtaaatc
    34981 ttctgatcga tacggcgacg cagttcggtc atatccaccg cctggacttt cattcccagc
    35041 caggattcaa agaagttgtg atcaacaata gaaccggcga tacccatcga aacgccgccc
    35101 agcgacagat agcttttacc tttcatgctg gcgacggcca aaccggcgcg ggcaaagcgc
    35161 agcagttttt cttcaacatc ggcaggaatc gatgtgtcat cggcatcctg aacgtcatga
    35221 ccgtaaatgg agaatgccgg gatgcctttc tggctgtgag ctgccagagc cgctgccagg
    35281 taaacagcgc cggggcgttc agtgccgtta aagccccaaa tggctttcgg gcgggttgga
    35341 tccatgtcga tggtttcact gccatagcac cagcaaggcg ttaccgtaat ggtgaggcct
    35401 acattctggc tgctgaattt ttcttcgcaa gcagcggctt cagccatacc cgcgatacag
    35461 gtatcggaaa tgacacactc gacggcagct ccgcaggtat ggcgcagttt ctcggtcagc
    35521 agtgcggccg tagctttcgc catattcatt gtttgttctt caagcgactc acgaacaccc
    35581 atgcgacgac cgtcaataac cgggcggata ccaattttcg gtaagctgat ttttttcatt
    35641 acagattcct cactttattc ggaaaatatg ttcagttagt tgccgtttga gaacggaaac
    35701 gggcaaagat aaagatgacc gcgaagcaga gtgcggggat cagttcagca gtggggatgt
    35761 tgcccgccgc gtcactgaca aaacccatga ccggagtgac aataccgccg ccaataatgg
    35821 tcataacgat gaaggacgaa ccgtatttgg tgtcctggcc gagattctta atgcccagcg
    35881 agaagattgt tgggtactga atcgacataa aggcgctgca taaagtcagg gctattaagc
    35941 ccacatgacc gccagcgaag gctgagatca ggcacagtgc catagcgatt aatgcgtagg
    36001 cggccaggac tttgtgtggt gcgaagcgac tgatgagcca ggtaccggtg atacgaccaa
    36061 taaagaagca caccatggtt ccggttaaat agttagcggc aaagcctgca gtcatacctg
    36121 gaatttcttc tacagcgtag cgaatcaaat agctccagca ggccgtttgt gcgccgacat
    36181 agcagaattg cgctaatacc gcccagcgcc agtgacgaat acgcgccaga cgagaaagcg
    36241 atgcggagaa cgatccttgt ttggcgtcac tgtgattatc actctgcaat gccgggaatt
    36301 tcgtcagcat gatcagcagg gcgaccagta acacgatagc cacgatgatc atataaggtg
    36361 tctgtaccga taatactagg ctgtgtttat acgcactcaa ttgctctgga gacattttat
    36421 cgagaacgtc ttgcgattga tgtggcacgt tagacaaaat aagactttgc ccaaagacaa
    36481 ccgcgataat tgcgc
//
LOCUS       DADLTF010000021        36432 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.021, whole genome shotgun sequence.
ACCESSION   DADLTF010000021 DADLTF010000000
VERSION     DADLTF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36432
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.021"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(24..278)
                     /locus_tag="JN749_001031"
     CDS             complement(24..278)
                     /locus_tag="JN749_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HAY7367524.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="JN749_001032"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="JN749_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HAY7367525.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="JN749_001033"
     CDS             1228..2694
                     /locus_tag="JN749_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAY7367526.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNPLKI"
     gene            2812..3798
                     /locus_tag="JN749_001034"
     CDS             2812..3798
                     /locus_tag="JN749_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAY7367527.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="JN749_001035"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="JN749_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HAY7367528.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="JN749_001036"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="JN749_001036"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HAY7367529.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="JN749_001037"
     CDS             5709..7265
                     /locus_tag="JN749_001037"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7367530.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="JN749_001038"
     CDS             7347..9161
                     /locus_tag="JN749_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7367531.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="JN749_001039"
     CDS             9162..10256
                     /locus_tag="JN749_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HAY7367532.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="JN749_001040"
     CDS             10256..11281
                     /locus_tag="JN749_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7367533.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="JN749_001041"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="JN749_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HAY7367534.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     LSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="JN749_001042"
     CDS             complement(12876..13220)
                     /locus_tag="JN749_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367535.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JN749_001043"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="JN749_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HAY7367536.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JN749_001044"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="JN749_001044"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HAY7367537.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="JN749_001045"
     CDS             15616..17376
                     /locus_tag="JN749_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAY7367538.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="JN749_001046"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="JN749_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HAY7367539.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JN749_001047"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="JN749_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HAY7367540.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="JN749_001048"
     CDS             19113..19340
                     /locus_tag="JN749_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HAY7367541.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="JN749_001049"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="JN749_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HAY7367542.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="JN749_001050"
     tRNA            21195..21271
                     /locus_tag="JN749_001050"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JN749_001051"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="JN749_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HAY7367543.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="JN749_001052"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="JN749_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HAY7367544.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /locus_tag="JN749_001053"
     CDS             complement(25143..26195)
                     /locus_tag="JN749_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HAY7367545.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JN749_001054"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="JN749_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HAY7367546.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /locus_tag="JN749_001055"
     CDS             complement(26746..28689)
                     /locus_tag="JN749_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7367547.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JN749_001056"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="JN749_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HAY7367548.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JN749_001057"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="JN749_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HAY7367549.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JN749_001058"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="JN749_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HAY7367550.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JN749_001059"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="JN749_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HAY7367551.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JN749_001060"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="JN749_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HAY7367552.1"
                     /translation="MLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JN749_001061"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="JN749_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HAY7367553.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JN749_001062"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="JN749_001062"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HAY7367554.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JN749_001063"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="JN749_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HAY7367555.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JN749_001064"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="JN749_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HAY7367556.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..>36432)
                     /gene="napA"
                     /locus_tag="JN749_001065"
     CDS             complement(34052..>36432)
                     /gene="napA"
                     /locus_tag="JN749_001065"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HAY7367557.1"
                     /translation="EAIKWDKAPCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLN
                     CIKGYFLPKIMYGKDRLTQPLLRMKNGKYDKEGEFTPITWDQAFDVMEEKFKTAMKEK
                     GPESIGMFGSGQWTIWEGYAASKLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEP
                     MGCYDDIEQADAFVLWGANMAEMHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELAD
                     NGIIFTPQSDLVILNYIANYIIQNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKA
                     AKNPGSDASEPMSFEDYKAFVAEYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYW
                     TMGFNQHTRGVWANNLVYNLHLLTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLP
                     ADMVVTNEKHRDICEKKWNIPSGTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQA
                     GPNINEERMPGWRDPRNFIIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQF
                     WRQQVQAPGEAKSDLWQLVQFSRRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVS
                     KFPVSELAEDQLNDESRELGFYLQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPV
                     VNGKETQWRYSEGNDPYVKAGEGYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLST
                     GRVLEHWHTGSMTRRVPELHRAFPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISI
                     VETRGRNRPPQGLVYMPFFDAAQLVNKLTLDATDPLSKETDFKKCAVKLEKV"
BASE COUNT         8870 a         9868 c         8992 g         8702 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccatc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcggccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaacc ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gctcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttgtg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcctggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcaatg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gccaggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc ggcagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact tggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcgcccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggca tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgcct atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcgc tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctag gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcg tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatgct gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcgcttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaaca
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cagcgcactg atgaccatcc
    13861 acgctgccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgaccccccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagaca ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ttggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcggt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atagcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ctttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggtc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata atgaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgacggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcaacaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gtcaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaggtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcca gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggt
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtacctctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc aaggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgttg gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac ggttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcatggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ct
//
LOCUS       DADLTF010000022        35807 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.022, whole genome shotgun sequence.
ACCESSION   DADLTF010000022 DADLTF010000000
VERSION     DADLTF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.022"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..218)
                     /locus_tag="JN749_001066"
     CDS             complement(<1..218)
                     /locus_tag="JN749_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7367558.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAEVY"
     gene            complement(219..305)
                     /locus_tag="JN749_001067"
                     /pseudo
     CDS             complement(219..305)
                     /locus_tag="JN749_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(399..1874)
                     /locus_tag="JN749_001068"
     CDS             complement(399..1874)
                     /locus_tag="JN749_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7367559.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3305)
                     /locus_tag="JN749_001069"
                     /pseudo
     CDS             complement(2021..3305)
                     /locus_tag="JN749_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="JN749_001070"
     CDS             complement(3418..5352)
                     /gene="yeaG"
                     /locus_tag="JN749_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HAY7367560.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGTLCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILPRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5788..6534
                     /locus_tag="JN749_001071"
     CDS             5788..6534
                     /locus_tag="JN749_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HAY7367561.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6624..7385
                     /locus_tag="JN749_001072"
     CDS             6624..7385
                     /locus_tag="JN749_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAY7367562.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7436..8320)
                     /locus_tag="JN749_001073"
     CDS             complement(7436..8320)
                     /locus_tag="JN749_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HAY7367563.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="JN749_001074"
     CDS             complement(8404..9399)
                     /gene="gapA"
                     /locus_tag="JN749_001074"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7367564.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9741..10154
                     /gene="msrB"
                     /locus_tag="JN749_001075"
     CDS             9741..10154
                     /gene="msrB"
                     /locus_tag="JN749_001075"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAY7367565.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10196..10468
                     /locus_tag="JN749_001076"
     CDS             10196..10468
                     /locus_tag="JN749_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HAY7367566.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10838..11914
                     /locus_tag="JN749_001077"
     CDS             10838..11914
                     /locus_tag="JN749_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7367567.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11941..13320
                     /locus_tag="JN749_001078"
     CDS             11941..13320
                     /locus_tag="JN749_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367568.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     SLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            13337..14382
                     /locus_tag="JN749_001079"
                     /pseudo
     CDS             13337..14382
                     /locus_tag="JN749_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14403..15239
                     /locus_tag="JN749_001080"
     CDS             14403..15239
                     /locus_tag="JN749_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAY7367569.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15244..16192
                     /locus_tag="JN749_001081"
                     /pseudo
     CDS             15244..16192
                     /locus_tag="JN749_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            16202..17182
                     /gene="ydjG"
                     /locus_tag="JN749_001082"
     CDS             16202..17182
                     /gene="ydjG"
                     /locus_tag="JN749_001082"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HAY7367570.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17319..18077
                     /locus_tag="JN749_001083"
     CDS             17319..18077
                     /locus_tag="JN749_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7367571.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18195..19553
                     /locus_tag="JN749_001084"
     CDS             18195..19553
                     /locus_tag="JN749_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367572.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="JN749_001085"
     CDS             complement(19646..20287)
                     /gene="pncA"
                     /locus_tag="JN749_001085"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HAY7367573.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="JN749_001086"
     CDS             complement(20298..21314)
                     /gene="ansA"
                     /locus_tag="JN749_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAY7367574.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TLDD"
     gene            complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="JN749_001087"
     CDS             complement(21481..23337)
                     /gene="sppA"
                     /locus_tag="JN749_001087"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAY7367575.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23498..24049
                     /locus_tag="JN749_001088"
     CDS             23498..24049
                     /locus_tag="JN749_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HAY7367576.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24166..25209
                     /gene="selD"
                     /locus_tag="JN749_001089"
     CDS             24166..25209
                     /gene="selD"
                     /locus_tag="JN749_001089"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HAY7367577.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25214..27175
                     /locus_tag="JN749_001090"
     CDS             25214..27175
                     /locus_tag="JN749_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAY7367578.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27302..28342
                     /locus_tag="JN749_001091"
     CDS             27302..28342
                     /locus_tag="JN749_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7367579.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQLGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="JN749_001092"
     CDS             complement(28459..29802)
                     /gene="gdhA"
                     /locus_tag="JN749_001092"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAY7367580.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30038..30310
                     /locus_tag="JN749_001093"
     CDS             30038..30310
                     /locus_tag="JN749_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HAY7367581.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="JN749_001094"
     CDS             complement(30276..30683)
                     /gene="nudG"
                     /locus_tag="JN749_001094"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HAY7367582.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30770..31417
                     /locus_tag="JN749_001095"
     CDS             30770..31417
                     /locus_tag="JN749_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAY7367583.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(31426..32733)
                     /locus_tag="JN749_001096"
     CDS             complement(31426..32733)
                     /locus_tag="JN749_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HAY7367584.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNGKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPVGGWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPGSSK"
     gene            complement(32800..33453)
                     /locus_tag="JN749_001097"
     CDS             complement(32800..33453)
                     /locus_tag="JN749_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7367585.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33453..34097)
                     /locus_tag="JN749_001098"
                     /pseudo
     CDS             complement(33453..34097)
                     /locus_tag="JN749_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(34054..34535)
                     /locus_tag="JN749_001099"
                     /pseudo
     CDS             complement(34054..34535)
                     /locus_tag="JN749_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(34565..34987)
                     /locus_tag="JN749_001100"
     CDS             complement(34565..34987)
                     /locus_tag="JN749_001100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367586.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTRWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGSVWASPLQ"
     gene            complement(34960..>35807)
                     /locus_tag="JN749_001101"
     CDS             complement(34960..>35807)
                     /locus_tag="JN749_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7367587.1"
                     /translation="KEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGA
                     QLTFIARRDVTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPA
                     ALKEAPDDATFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTF
                     NPAHAQQKIASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDA
                     QLRKADPVVWGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLR
                     RYGTH"
BASE COUNT         9081 a         8479 c         9163 g         9084 t
ORIGIN      
        1 cagtacacct cagcgagagc gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 ctcatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gcgtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaat atttcattaa catgaaaaga
     1561 aaaatgattg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgattgt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgcaca caatgtctga
     2161 tgtgcacgac gggtaatttc gatatagctg taataacgaa caacaggtaa taattttttc
     2221 gccaggattt catggcaaag cggagagtca tcggcccagt tatcgccgtc cgatgcttgt
     2281 gcagcgtaaa tattccactg tgccgggtta taacgctctt tcactacctc atccatcagt
     2341 ttcagggcgc tggaaacaat ggtgccgcct gtttcctgcg agtagaaaaa ctcatgttca
     2401 tcgacttctt tcgcctgggt atgatggcgg atgtatacga cttccacgtt cttatacgtt
     2461 ctgctgagga acagatacag cagaatataa aaacgcttag ccatatcttt agtggattga
     2521 tccattgaac cggaaacgtc catcaggcaa aacatcactg cctggctgga gggatcgggc
     2581 cgcttctcgt agttcttgta acgtaaatcg aaggtgtcaa taaaagggac gcgttcaatt
     2641 ttggcacgta attctgcaat ttctttgcgc agacgttcct cttccagcag ctgcgcaggt
     2701 tcactgttgc tgatgatggc caaattctct tccagtgcat gaagttcccg ccgcttgcct
     2761 gccgtcatgg ctgtgcgtcg cgccagtgag ttctgcaagg aacgcacaac gctgatattg
     2821 gctggaacgc cgttagcggt ataacccgcc cgatgcgttt tatattcggt cagctggcgt
     2881 tgttggtttt gtttcagatt cggtaaggcc aaatcttcaa agagcagatc aagatactca
     2941 tctttcgaaa tctgaaagac aaattcatcc tgaccttcac catcctggct ggcctggccc
     3001 tgaccactgc cggaacctcc gccgccaccc tggggacgtt caattcggtc gttctgaacg
     3061 aaatggtcat tgcccggatg cacgcggtgg cgcagaccgc cacgcccctg atgaaacatt
     3121 ggttcgctaa tatcttccgt gggaatggat acggattcgc tgctgtcgac gtcagtcacc
     3181 gaacgcttat taatggcctc ggagatcgac tgtttaattt gcgctttata acggcgtaaa
     3241 aaacgctggc gattcaccat gcttttgttt ttgccgttca gacgccggtc aataaaccag
     3301 gtcatatgcc ccccgtacta catttgccaa ctgtatgcta cgcctgtgtt gccgcttctc
     3361 aggcccacgg ttcgctatcg ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta
     3421 agacgattta cgtacgcgca aataccattc gcacagtaaa cgcacctgtt tacgggtgta
     3481 gcctttttcc atcatacggt cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt
     3541 tttggcgtta aacgagataa ccggcaacag ctcctcggta ttggagaaca ttttcttctc
     3601 gataaccgtg cgcagttttt catagctggt ccagttcgga ttgcgtccgc tgttattcgc
     3661 tctggcgcgc agtacgaagt taacaatctc gttgcggaaa tcttttggat tactgatccc
     3721 cgccggtttc tcgattttct ccagctcggc gttaagagac tcgcggtcaa acagctgccc
     3781 ggtatccgga tcgcgatact cctgatcctg aatccagaaa tccgcgtagg taacataacg
     3841 gtcgaaaatg ttttgcccat attcggaata ggattcaagg taggccgtct ggatctcttt
     3901 gccgataaac tcggcatatt tcgggatcag ataacctttc aggaactcca gatagcgttc
     3961 tgcctgctct tgcgggaact gctcgcgctc gatctgctgt tccaggacgt agaacagatg
     4021 gaccgggttt gccgccactt ctacatgatc gaagttgaac acgcggggga ggatcttaaa
     4081 cgcaaaacgc gtcgacagac cgttcatccc ttcatcgaca ccggcgtagt cacgatattc
     4141 ctgatacgac ttggctttcg gatcggtgtc tttcagactt tcgccatcat aaacccgcat
     4201 ctttgaataa atgctggagt tttctggctc tttcaggcgc gaaagaatgg aaaaacgtga
     4261 cagtgtttcg agcgtgccag gggcgcatgg ggcgtgagtc aattcactgt gattaagcaa
     4321 tttctcgtag attttgatct cttcggaaat gcgcaagcaa tacggcacct tcacgatgta
     4381 aacacggtcg aggaaggctt cgttgttttt gttattacgg aaagtgaccc attcggactc
     4441 gttcgagtgg gcgagaataa tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt
     4501 gtagttatct tcctgagtgg cggttaacaa gggatgcagc acttttatcg gtgctttaaa
     4561 catctcaacg aattccatga tcccctgatt ggcgcggcac agcgtaccgg aatagccgta
     4621 ggcgtccggg tcattctgcg cgtagtgttc gagtttacga atatcgactt tcccaaccag
     4681 cgcggagatg tcctggttgt tctcatcacc gggttccgtt ttggcgatag caatttgttg
     4741 cagaattgac ggccagacct tcactacccg gaacttagtg atatcgccac caaattcatg
     4801 caggcgtttt gccgcccatg gcgacatgat ggtgccgaga taacggcgag gaatgccata
     4861 ctctttttcc agaatctgag catcttcctg cggattgaaa agacagaacg gatgatcgtt
     4921 gaccgggcta cgctcaccgt tggcgctcaa tacataaatc ggtacgagct gcattaatga
     4981 tttcagtcgc tcagcaagcg atgatttacc cccacccaca ggccccagca gatacaggat
     5041 ttgtttcttc tcttccagcc cctgagccgc gtgtttcaga taagagacaa tctgttcaat
     5101 cgcgtcttcc atgccgtaaa actcttcaaa tgccggataa cgtgcaatga cccggttaga
     5161 aaagagtcga gaaagtctgg gttcctgggc tgtatcgacc atgacaggct caccgatagc
     5221 catcaatagc cgctcagccg cgttggcata agcactgcga tcttgccgac aagtggtaag
     5281 aaactcctgc agtgtgaact cttcgtcctt ggcagcttca tatcgctggc gatagtgatc
     5341 gaatatattc atggtatgcc gtcctttcgt tttttagcac aggttaagag ccgttcatat
     5401 gaatagtaga ggctcccgga agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa
     5461 ctctcatgcc attttatcga cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta
     5521 agcgtagatg gcaaatggaa aacttgcctg cacggctttc ttaatttcaa tggcatatca
     5581 acaactcatc cataaatttt gcataattaa tgtaaagacc aggctcgcca gtaacgctaa
     5641 atccatttgg ctgtaagcgc ggtgtcatcc gcggcaggaa aattaaacag ttactttaaa
     5701 aaatgaaaac gtaaaaaggt tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct
     5761 aaacatttgt ttaaggaatg attaattgtg accaaactca aacttctggc acttggagtg
     5821 cttatcgcaa cgtctgcagg cgtagcgcac gctgaaggta aattttccct gggcgcaggc
     5881 gtaggtgtcg ttgagcaccc atataaagat tacgataccg atgtttaccc agtaccggta
     5941 atcaactatg aaggcgataa cttctggttc cgtggcttag gtggtggtta ctacctgtgg
     6001 aatgacgcaa cggataaact ttcaattacc gcttactggt cgccgcttta cttcaaagcg
     6061 aaagacagtg gcgatcacca aatgcgtcac ctggatgacc gtaagagcac catgatggct
     6121 ggtctgtctt atgctcactt tacccagtac ggttacctgc gtaccaccct ggctggcgat
     6181 accctggata acagcaacgg cattgtctgg gatatggctt ggttgtatcg ttacaccaac
     6241 ggtggcctga ccgtgactcc gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa
     6301 tactattatg gcgtatcgcg caaagagtcc gctcgcagcg gtctgcgtgg gtataaccca
     6361 aatgacagct ggagccctta ccttgagctg agcgccagct acaacttcct cggcgactgg
     6421 agtgtttacg gtaccgcgcg ctacacccgt ctgtctgatg aagttactga cagcccgatg
     6481 gtggataaat cctggactgg cctgatttct accgggatca cctacaaatt ctgataatgc
     6541 atctttccag ggcattttgt gcataaaaat agggcgtcac gccctgtttt gcattacggt
     6601 ggtgcaatac ctgggaggaa acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct
     6661 cactgccagc catagggcag ggaacatggt atatgggcga agatgccagt cagcgcaaaa
     6721 cagaagttgc tgcactacgc gcaggcattg aactcggttt aaccctcatt gataccgccg
     6781 aaatgtatgc cgatggcggt gccgaaaagg tggttgggga agcattaacc ggtctgcgag
     6841 agaaggtctt tctcgtctct aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa
     6901 atgcatgcga agccagttta cgccgtctca atactgatta tctcgatctc tacttattac
     6961 actggtctgg cagtttcgct tttgaagaga ctgttgctgc gatggaaaaa ttgatcgccc
     7021 agggaaaaat ccgtcgctgg ggcgtttcta accttggtta tgccgatatg caggaactct
     7081 ggcagctgcc ggggggaaat cagtgtgcca ctaatcaggt gctttaccat ctcggttcac
     7141 gagggattga gtacgatcta ctcccctggt gccagcaaca gcagatgccg gtgatggctt
     7201 acagtccatt agtccaggcc gggcggttgc gcaatggact gttaaaaaac gcggtagtca
     7261 acgaaattgc acatgtccaa caaaatgcag ctgcgcttga ggtcgaactt tcgtcagcgg
     7321 aattagctat gctggataag gcatatccgg caccaaaagg aaaaactgcg ctggatatgg
     7381 tgtgatgttg atacgtgccg gatgcatcgc acccggcaca caacattaac gcaaattaac
     7441 gtttggcaac gcgaatggat tgcgccagat gtgcaggttt ctctttggtg actttttgcg
     7501 tttctgaagc gtaagccgtt tctacacaaa caaaggtctt gtagccatcg tccggcatgt
     7561 cgcccatgct aactgaaagc gccggtcccg ggttccagcc gacaacgttc agatgatgct
     7621 ggtggcctac ggcgataata cgattcagcg cttcatcatt aatcacgctg caatcttgcg
     7681 gattcagata cacgcgatcg gtacggtcag ggaaggtctg aataccgtcg gtcagtacat
     7741 cttctttcgc gtcattcact ttatcaatga agcgatcgcc cagcccactg acgcttacct
     7801 tagcgatatc acccacgtta aagtaggtat gcagggcaga ggtggtttca aattcgccat
     7861 gtgattcaag atcgatttca caggttttac ccacgcggaa atgcgctaac agcgtaaagt
     7921 cgtgcggcca gaattttttc gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc
     7981 catcagcatc ttcatgatgt gatttcagcg tccacggcag gttgcgggca aaaccgtgcg
     8041 caggcagacc ttgttgtgcc gccggaccaa accacggcca gcaaaccggt acgctaccgc
     8101 gaatagcgac gccatttttg aacggtgtgt tgttgctcaa ccacagaact tcttcttcac
     8161 ccgcaggttt ccacgagaga aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg
     8221 gatgatcgac cacaatgagg tccagttcat ccagtttacg acgggagagg acaggggaga
     8281 tttgttcgat gaccggaagg gcaaaaattt tcttaatcat gacgcagtcc tttaacttca
     8341 ttttatcagg taaaaaaaag agcgaccgaa gtcgctcttt ttagatcaca gtgtcatctc
     8401 aacttatttg gagatgtgag cgatcaggtc cagaactttg ttggagtaac cggtttcgtt
     8461 gtcgtaccaa gataccagtt tcacgaagtt gtcgttcaga gcgataccag ctttagcatc
     8521 gaacacggaa gtgcaaactt cgccgttgaa atcggtagat actacgtcat cttcggtgta
     8581 gcccagaacg cctttcattt cgccttcagc agcagcttta acggcagctt tgatctgctc
     8641 gtaagttgca gctttttcca gacgaacggt caggtcaact acggatacgt tcggggtcgg
     8701 aacgcggaac gccataccag tcagtttgcc attcagttct ggcagtactt tacctacagc
     8761 tttagcagca ccggtagagg acgggatgat gttctgggaa gcgccgcggc cgccgcgcca
     8821 gtctttgtga gacgggccat caacggtttt ctgagtagcg gtagtagcgt gaacggtggt
     8881 catcagacct tcgatgatgc cgaagttatc gttgataact ttagccagcg gagccaggca
     8941 gttggtggtg caggaagcgt tggaaacgat gtcctggcca gcatatttgt cgaagttagc
     9001 gcctttaacg aacatcggag tgttgtcttt agacggacca gtcataacca ctttcttcgc
     9061 accagcggtg atgtgtttac gagcagtttc gtcagtcagg aacagaccag ttgcttcagc
     9121 gacaacgtca acacccactt cgtcccattt caggttagcc ggatcacgtt cagcggtaac
     9181 acggattttt ttaccgttaa cgatcagatg accgtctttc acttcaacgg taccgtcgaa
     9241 acggccgtga gtggagtcat atttcagcat gtatgccatg taatcagcgt ctaacaggtc
     9301 gttgattgca acgatctcga tgtcagaacg tttctgagca gcacggaaaa caatgcgacc
     9361 gatacggcca aaaccgttga tacctacttt gatagtcata tattccacca gctatttgtt
     9421 agtgaataaa aggttgcctg taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt
     9481 gtcaatcatt gcgacagatc aatcctgtgc ctaagcatta cgcgactgac tcgcctcact
     9541 cttcctttgg gcttgagacc acatggggtc ggcgccccga attttaaagg gcaattacga
     9601 taaaaatgtg attttcatca cgatttaggt gcagccattt cgtcgagatc aagtttagga
     9661 caaaattttg ccagttggtg ttaatgtttt gttagaatca gtcacgtaat gtgagcacga
     9721 ttaaagtgag atgtgagtaa atggcgaata aaccttcggc agaagaactg aaaaaaaatt
     9781 tgtccgagat gcagttttac gtgacgcaga atcatgggac agaaccgcca tttacgggtc
     9841 gtttactgca taacaagcgt gacggcgtat atcactgttt gatctgcgat gccccgctgt
     9901 ttcattccga aaccaagtat gattccggct gtggctggcc cagtttctac gaaccggtga
     9961 gtgaagaatc cattcgttat atcaaagact tgtcacatgg aatgcagcgc atagaaattc
    10021 gttgcggtaa ctgtgatgcc catctggggc atgtctttcc cgacgggccg caaccaacag
    10081 gcgaacgtta ttgtgttaac tctgcttctt tacgcttcac tgatggcgaa aacggcgaag
    10141 aaatcaacgg ttgaaaaaac gattcagcaa cttattccac aggagcggaa aaattatgaa
    10201 tcttgatgac attatcaaca gcatgacgcc tgaggtatac cagcgtttgt cgaccgctgt
    10261 tgaactgggg aaatggcctg atggcgttgc gttgaccgag gaacaaaaag agaattgcct
    10321 gcaactggtg atgctgtggc aagcccgcca taataccgaa gcacagcata tgacaattga
    10381 cacaaatggt cagatggtca tgaagagcaa acagcagtta aaagaagatt ttggtatctc
    10441 agcaaagcct attgccatgt ttaagtaagt ggttcatccc ctttttgccg cggctatgaa
    10501 ggggatagcc aacaatgacc ccacgactcg tctcaattat ctcgacccga cctggctaag
    10561 tcccagtccg atctcttgca gtcgcgttca cgcctgaatg acttctcctg aacttaagtc
    10621 tcagacctat ttggccggta atccctctcg aatttgctct ttttgcgtcc cgccttccac
    10681 caacctgtaa cagacccagc cattttctgc gtaattgtga tctaagttgt aaaatgcagg
    10741 tcattatctg tgttgtgcag atttaatgtt gacctcaaaa atcatgtggg taagtatgat
    10801 ggccatctaa gcaaaacaaa acacagagga tgacgcaatg aaagcactgg ctcggtttgg
    10861 caaggccttt ggcggctaca agatgattca tgtcccacaa cccatttgtg gcccggaaga
    10921 cgtagtgatt gaaattaaag ccgcggcaat ctgcggcgca gacatgaagc actacaatgt
    10981 cgatagcggt tctgatgagt ttaactctat ccgcggccat gagttcgcag gttgtattgc
    11041 gcaggttggt gaaaaagtca aagactggaa agtggggcaa cgcgtcgtgt cggataacag
    11101 cggtcacgtt tgcggcgttt gtccggcctg tgaacagggt gattttctgt gttgtacaga
    11161 aaaagtaaac cttggtctgg ataacaatac ctggggcggt ggtttttcca aatattgtct
    11221 ggttcctggt gaaattctca aaattcatcg tcatgcgttg tgggaaatcc ctgatggtgt
    11281 tgattatgag gacgcagccg tacttgaccc tatctgtaat gcctataaat ccatcgcgca
    11341 gcaatcgaaa ttccttcctg gtcaggatgt tgtcgtcatt ggcactggcc cactcgggct
    11401 gttttccgta caaatggcgc ggattatggg ggcggtaaat atcgtcgtcg ttggtctgca
    11461 agaagatgtg gcggtccgct tcccggttgc aaaagaactg ggggcgacgg cagtagtaaa
    11521 tggttctacc gaagatgtgg tggcgcgctg ccagcaaatt tgtggcaaag ataatctggg
    11581 actggtgatt gaatgctccg gtgccaatat cgcactgaaa caagccatcg aaatgctccg
    11641 cccgaatggg gaagtggtac gcgttggaat gggcttcaaa cctcttgatt tctcgattaa
    11701 tgacattacc gcctggaata aaagcatcat tgggcatatg gcctatgact ccacctcatg
    11761 gcgtaacgct atccggctat tagccagcgg cgctatcaaa gtcaaaccga tgatcacgca
    11821 tcgtatcggc ctgtcgcaat ggcgcgaagg gtttgatgcg atggtcgata aaaccgcaat
    11881 caaagtgatc atgacttacg actttgatga ataactactc tcctgaagga acgttatcgc
    11941 atggaacaga taacaaaacc gcattgtggt gcacggctgg atcgcttacc ggattgccgc
    12001 tggcattcgt caatgtttgc catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc
    12061 gttggtggct tgatcctcgc gcagctgaaa gcgttgggct ggacagataa ttccaccaca
    12121 gccacattct cagcaatcac gaccgccgga atgtttctcg gtgcgctggt gggcggcatc
    12181 attggcgaca aaaccggtcg cagaaatgcg ttcatcctct atgaggccat tcatattgcc
    12241 tcgatggttg tcggtgcttt ctcaccgaat atggattttc ttattgcctg ccgttttgtg
    12301 atggacgttg ggctgggagc tttactggtt acactgtttg ctggtttcac cgaatatatg
    12361 cccggtagaa atcgcggaac gtggtccagt cgggtttcct ttattggcaa ctggtcatat
    12421 tcgctctgct cactgattgc gatgggactc acgccgctga ttagtgcaga gtggaactgg
    12481 cgggtacaac tgcttattcc tgcaatattg tcgcttatcg ctacggcgct ggcctggcgc
    12541 tactttcctg aatccccgcg ctggctggaa tcgcgcggac ggtatcagga agccgagaaa
    12601 gtcatgcgga gtatagaaga aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg
    12661 gttattgctg atgacggtaa agcgccacaa gcggtgccgt attcagcctt actgacagga
    12721 gtattactga aacgcgtgat attaggctct tgtgtgctga ttaccatgaa cgttgtgcag
    12781 tacacactaa ttaactggtt gccaacaata ttcatgaccc aggggattaa tttaaaagac
    12841 tcgattgttt taaataccat gagtatgttt ggcgcgccat ttggtatttt tattgccatg
    12901 ctggtgatgg ataaaattcc gcgtaaaaca atgggtgtgg ggctattaat cctgattgcg
    12961 gtgctcggat atatctattc actgcaaacc agtatgttgc tcataacgct gattggtttc
    13021 ttcctgatta ctttcgtcta tatgtacgtt tgctatgcct cggcagtgta tgtccctgaa
    13081 atctggccga cagaggccaa actccgtggc tccggtctgg cgaatgcggt agggcgaatc
    13141 agtggtattg ccgcacctta tgccgttgca gtgctgctca gtagttatgg tgtaacggga
    13201 gtctttattc ttctgggggc tgtttcaatt attgtcgcaa ttgctatcgc caccattgga
    13261 attgaaacca aaggtgtctc cgttgaaagt ttaagtattg atgcagtagc caataaataa
    13321 tacgggtgga ttcaaaatga aaaattcaaa agcaatattg caggtgccgg gcacaatgaa
    13381 aattatttca gcagaaatac cagtgcctaa agaagatgaa gttttgatta aagtagaata
    13441 tgtcggtatt tgtggttcag atgtacatgg ttttgaatca ggcccgttta ttccgcctaa
    13501 agacccaaat caagaaattg gcctgggtca tgaatgcgcc gggacggttg tggctgtggg
    13561 aagccgcgtg cgcaaattta aaccggggga tcgggtaaat atcgaacctg gcgttccttg
    13621 cggtcactgt cgttactgtc tggaaggcaa atataacatc tgcccggacg ttgattttat
    13681 ggcgacacaa cccaactacc gcggcgcatt aacgcactat ctgtgtcatc cggagagctt
    13741 tacttacaaa ctgcccgaca atatggacac gatggaaggg gcgctggtgg agcctgccgc
    13801 agtcgggatg catgccgcga tgctggcaga tgttaaaccg ggtaagaaga taattattct
    13861 gggagcaggt tgtattggtt tgatgacgtt gcaagcgtgc aaatgcctgg gcgcaacgga
    13921 aattgccgtc gttgatgtac tggaaaaacg tctggcaatg ggggaacagc ttggtgcgac
    13981 agtggttatt aatggcgcaa aagaggacac tattgcacgc tgtcagcaat ttaccgaaga
    14041 catgggcgca gatattgttt tcgaaacagc gggttctgcg gtcaccgtta aacaggcacc
    14101 tttaatctgg taatgcgcgg cggtaaaatt atgattgttg gtactgtacc cggcgattcg
    14161 gcaatcaatt tcctcaaaat caatcgcgaa gtcactatcc agacggtatt ccgctatgcc
    14221 aatcgttatc cggtcacgat tgaagctatt tcttcagggc gattcgatgt gaaatcgatg
    14281 gtgacgcata tttacgatta tcgggatgta caacaggcat ttgaagagtc agttaacaac
    14341 aagcgcgaca ttattaaagg cgttattaaa attagcgatt aattaatacg ggagaacaga
    14401 acatgctcgc agatatcaga tattgggaaa acgatgccac aaataaacat tatgcaattg
    14461 cccattttaa cgtatggaat gcagaaatgt tgatgggcgt taaagacgca gccgaagaag
    14521 cgaaatcccc ggttattatt tcttttggta caggttttgt cggtaacacc tcatttgaag
    14581 atttctctca catgatggta tcaatggcac aaaaagcaac ggtgccggta ataactcatt
    14641 gggatcatgg tcggagtatg gagattattc ataacgcctg gactcatggt atgaattcat
    14701 taatgcgtga tgcttctgca tttgatttcg aagaaaatat tcgtttaacc aaagaggctg
    14761 ttgatttctt tcatccgctg ggtattccgg tagaggcgga attagggcat gtcggtaatg
    14821 aaaccgttta tgaggaagcg ctggcgggct atcactatac cgatcctgac caggcggctg
    14881 aatttgttga acgtacgggc tgtgactcat tggctgtcgc catcggcaac cagcatgggg
    14941 tttatacgtc agagccgcaa ttgaactttg aggtcgtcaa acgcgtacgc gatgctgttt
    15001 ctgttccgct ggttttgcat ggcgcatcgg ggatcagtga tgccgacatt aaaactgcaa
    15061 tttcgttggg tattgcgaaa atcaacattc atacggagct ctgtcaggcc gcaatggtcg
    15121 cagtgaaaga aaaccaggat cagcctttcc tgcatttaga acgtgaagta cgtaaagctg
    15181 taaaagaacg ggcattggaa aaaattgaac tgttcggttc tgatggcaaa gcggaataat
    15241 gatatggata atctcgacgt tatttgtata ggtgccgcta ttgttgatat tccattacaa
    15301 ccggtcagta aaaatatctt tgatgtagat tcttatcctc ttgaaagaat cgcaatgagc
    15361 accggggggc gatgcaataa atgaagcaac aattatttct cgtctggggc atcgtaccgc
    15421 attaatgagc cgcattggta aagatgcctc agggcaattt attctcggcc attgtcgcaa
    15481 agagaatatt gatattcaaa gcctgaaaca ggacgtcagc atagatacat ctattaacgt
    15541 tggactggtg acggaggatg gcgagcggac gtttgtcacg aaccgtaacg gcagtttgtg
    15601 gaaattaaat attgacgatg ttgattttgc gcggtttcct caggcgaaat tattatcact
    15661 ggccagtatt ttcaacagtc ctctattgga tggtaaagcg ctaacagaaa tttttacgca
    15721 agcgaaagcc cggcagatga tcatttgtgc cgatatgatc aaaccgcggt tgaatgaaac
    15781 gctggatgat atttgcgaag cattgagtta tgtcgattat ctgtttccaa attttgccga
    15841 ggcaaaatta ctcacaggga aagagacact ggatgaaatt gctgactgct ttcttgcgtg
    15901 cggcgtaaaa acggtggtga ttaaaacggg taaagacggc tgctttatca agcgtggtga
    15961 catgacgatg aaggtgccgg cggtcgcagg aataaccgcc atcgacacca ttggcgcggg
    16021 cgataacttt gcttcaggtt ttattgcggc actgttagaa ggcaaaaatc tgcgtgaatg
    16081 cgcacgcttt gccaatgcaa cggcggctat ctcggttcta agcgtcggtg ccaccaccgg
    16141 cgtaaaaaac agaaagctgg tcgaacaatt gctggaagaa tacgaaggat aatgaaggca
    16201 aatgaaaaag atacctttag gcacaacgga tataacgctt tcgcgaatgg ggttggggac
    16261 atgggccatt ggcggcggtc ctgcatggaa tggcgatctc gatcggcaaa tatgtattga
    16321 tacgattctt gaagcccatc gttgtggcat taatctgatt gatactgcgc taggatataa
    16381 ctttggcaat agtgaagtta tcgtcggtca ggcgttaaaa aaactgcccc gtgaacaggt
    16441 tgtagtagaa accaaatgcg gcattgtctg ggaacgaaaa ggaagtttat tcaacaaagt
    16501 tggcgatcgg cagttgtata aaaacctttc cccggaatct atccgcgaag aggtagaagc
    16561 cagcttgcaa cgtctgggta ttgattacat cgatatctat atgacgcact ggcagtcggt
    16621 gccgccattt tttacgccga tagctgaaac tgtcgcagtg cttaatgagt taaaggctga
    16681 agggaaaatt cgcgctatag gcgctgctaa cgtcgatgct gaccatatcc gcgagtatct
    16741 gcaatatggt gaactggata ttattcaggc gaaatacagt attctcgacc gggcaatgga
    16801 aaacgaactg ctgccgctat gtcgtgataa tggcattgtg gttcaggttt attccccgct
    16861 agagcaggga ttgttgaccg gcaccatcac tcgtgattac gttccgggcg gcgctcgggc
    16921 aaataaagtc tggttccagc gtgaaaacat gctgaaagtg attgatatgc ttgaacagtg
    16981 gcagccactt tgtgctcgtt atcagtgcac aattcccact ctggcactgg cgtggatatt
    17041 aaaacagagt gatttaatct ccattcttag tggggctact gcaccggaac aggtacgcga
    17101 aaatgtcgcg gcactgaata tcaacttatc ggatgcagac gcaacattga tgagggaaat
    17161 ggcagaggcc ctggagcgtt aaatactcaa cacgattcac ctctgtttct gtgaaaactg
    17221 tgatgaaaat ctgtgattca aataggttat tttgaaagta aacatcggtt atgcgataat
    17281 cgcgctaatg tgatgtaaaa agatacaggg gtgtaatcgt ggcggcaaaa gacaggattc
    17341 aggcaattaa gcaaatggtt gccaacgata aaaaggtgac agtctcaaat ttgagtggga
    17401 tttttcaggt aaccgaagaa accattcgcc gcgatcttga gaagctggaa gatgaaggct
    17461 ttttgaccag aacctatggt ggtgctgttt taaatacagc ggtgttgacg gagaatatcc
    17521 atttttataa gcgcgcttca tcgttttatg aagagaagca gcttattgca cgcaaggcac
    17581 taccctttat cgacaataaa accaccatgg cagccgattc cagtagtacc gtaatggaat
    17641 tgctcaaatt gttacaggac cgtagtgacc tgacgttgct aaccaactcg gcggaagcga
    17701 ttcatgttct ggctcagtca gaaattaaag tcgtttcaac gggtggggaa ctaaacaaaa
    17761 atacactttc cctgcaagga agaattacta aagagatcat cagccgctac catgtcgata
    17821 tcatggtaat gagttgtaaa ggtcttgata ttaacagcgg cgcgctggac tctaatgaag
    17881 cggaagctga aatcaaaaag acaatgatcc gtcaggcgac agaagttgcg ttattggttg
    17941 atcactctaa gtttgatcgt aaagcttttg tccagttagc tgactttagt catattaatt
    18001 atataataac tgataaatca ccgggtgcag agtggatagc attttgcaaa gacaataata
    18061 tccagctggt gtggtaattt aagcacacgt tgtcaatatt atttgttcct gcgcctggaa
    18121 ataaaatgat gataaagatg cggcaagtga atttgccgca attgctacct tttatagcaa
    18181 atccgaggca cccaatggaa caatatgatc aaattggcgc aagactggac cgcttgcctt
    18241 tggcccggtt tcattatcgt atatttggta taataagctt tagtctgtta ttaacggggt
    18301 ttttgagtta ctcaggtaat gtcgtcttag caaagctggt aagcaatgga tggtcaaata
    18361 atttcctcaa tgccgccttt acctcggcat taatgtttgg ttatttcatc ggctcactta
    18421 ctggtgggtt tattggtgac tactttgggc ggcgcagggc gtttcgcata aatcttctca
    18481 tcgtcggtat tgctgcaaca ggggccgctt ttgtccctga tatgtactgg ctcattttct
    18541 tgcgcttcct gatgggaaca ggaatggggg cgctgattat ggttggctat gcctcattta
    18601 cggagtttat ccccgcgacg gtgcgtggaa aatggtccgc gcggctctca tttgttggta
    18661 actggtcgcc catgctgtct gcggcgatag gcgtggtggt tatcgctttt tttagttggc
    18721 gaataatgtt tcttctgggt ggtattggca tactgttagc ctggtttctc tcaggtaaat
    18781 actttatcga gtcgccacga tggctggcag ggaaagggca aatcgccggt gcagaaagcc
    18841 aacttcgtga agtagagcag caaattgaaa gggagaagag tattcgttta ccccagctta
    18901 ctttgaacca gagcaacagc aaggttaagg taatcaaggg taccttcagg ctcctgttta
    18961 aaggggaaat gttacgacgt acattagttg cgataactgt tttaattgca atgaacattt
    19021 cgctttatac cattaccgta tggataccga ccatatttgt taactccggc attgatgtcg
    19081 ataaatcaat attaatgacc gctgttatta tgattggtgc tccggtagga atatttattg
    19141 cggcattaat tattgatcat tttcctcgtc gattatttgg ctccgcctta cttattatta
    19201 ttgccgtgtt aggctatatc tattcaagtc agactacaga gtgggcgatt ttaatctatg
    19261 gtctggtgat gatcttcttt ttatacatgt atgtttgctt cgcgtcggcg gtttatatcc
    19321 cggagctttg gccaacgcat ttacgcctgc gcggttcggg tttcgttaat gccgtcggac
    19381 ggatcgtcgc agttttcacg ccctatggcg ttgcggcatt attaacacat tatgggtcga
    19441 tcacggtgtt tatggtgctt ggtgtcatgt tagtgctctg tgcgctggtt ctctccattt
    19501 ttggcatcga aacgcggaag gtgtcgctgg aagagatttc tgaggtgaat taaatctttc
    19561 gttgatggca tcaattgcca gatgtaccac tgacgtgttt tatttggcct acaatgccag
    19621 tacagaattg taggccagcg taaaattacc cctgcgtctc ttcccagtct gccagcgtat
    19681 atagcgttgc cccagctgct gacatctcca taaacgcgtg tgcactgtcc tggggctgga
    19741 tattcacgcc acgacagcca tcggtaatca cgtttacctt ataacctaac tgtaacgcgt
    19801 ccagcacggt aaacttcacg caatagtcag tagccaggcc cataacgatc aattcatcga
    19861 tttcatgatc gcgcaaccag tcatcaagcg cggttttctg ccgacgtccg ttatcaaaaa
    19921 aggcactgta actgtcaact aaaggatttt cgcctttatg gaacaccgct gcgatctctt
    19981 tttgattcag taacggatgt aattgtgcgc cttcactgtt ctgcacacag tgttcaggcc
    20041 agaaagtttg ccgcaggccg tcgagttggc ctggcgtata aggctctacg ccgtgctggc
    20101 tggcaaagct gccgtgattc gccggatgcc agtcctggct ggcgataacc gcttctccgc
    20161 gcgactggca ccagtcaata aggcggttag caacctccac cgtactgtca ccttccggta
    20221 cggcgagcgc gccaccagca cagaaatcat tttgtaaatc gaccagtaac agggcgcgat
    20281 ggggcattgc agtctcctta atcatccagc gtcagttcgc cgcgcaggtt ttggctcatg
    20341 gccttgcgaa tggtttcagt atccagttcc tggctcagca ggtaatgcag tttggttagc
    20401 gtggcttcta cagtcatatc tgcaccgcca ataacgccgg catgggcgag gacgttaccg
    20461 gtggcataac cactcatatt cactttaccg gacatacatt gtgtcaggtt gaccaccaca
    20521 ataccgcgat cgctggcttc ttgtaattcc tgcaggaagg ctttgttttg tggcgcatta
    20581 cccacgccat aggagcgcag aatcaatgct ttcaccggtt ggcgcagaaa attgcgcacg
    20641 acgtcagcag aaatccctgg ataaatcgtc actacgccaa ttggttgtgg cgtgattgga
    20701 tgaacgatta attccccttc accgtgcggg gcgggtggcg tattcaaacg acgaatatgg
    20761 ataccagctt ccagtaacgg aggaaggttt ggagaggcaa acgcatcaaa accatcggca
    20821 tgggctttgg tagtgcggtt gccgcgatac aatcggttat tgaaaaagag cgttacttcg
    20881 ttgatcggat aattcgccgc aacgtacaac gcattcagca gattaatttg tccgtcagag
    20941 cgtaactcag ccagcgggat ttgtgaccct gtcacaatga ccggtttacc gagattctcg
    21001 agcatgaacg acagcgcaga ggcggtatac gccatcgtgt cggtaccgtg tagaatgaca
    21061 aaaccatcat agtcgtcata gtgcgcttta atatcttcag caatatgctg ccagtcttcc
    21121 ggcgtcatat cagaagaatc catcaacggc gtatattcat gaatggtgaa atctggcatc
    21181 tccaggcgat ggaattccgg catcagcgcc agttggcgtt gtagatgacc tgacaccgga
    21241 atataaccct gctcggaacg ctgcatcccg atggtcccgc ccgtgtaggc gacgtaaatt
    21301 gatttctttt gcatgatatt gatatgaatg atatcgaaag agcaaaagta tatacgtgag
    21361 gcaggaggag cgcacctgat actatgcgga taaggaaata tggggcgaga cagcgggaag
    21421 aggattcgtc gaggtaaaaa ataatgtggc cgacgatctg tcggccactc agtacaagac
    21481 ttaacgcacg ttggcgcagg tcaggcaaaa cgcataacgg ttttgtgggt cgttaaacgc
    21541 ggccagcttg tcgctttcac ttttaacggt agaggctacc gaggcaagcg gtgcaggtaa
    21601 catggcctgg aacgcatctg gcaacattgc ccggacagaa ccagacatgt tgtccatcac
    21661 tttgtcgaag aaggtcggtt catcaacgta gtattccaga tgccactgtt tcacttttgc
    21721 gagctctgct gctttggcaa ccgcatcatc gaaatccccg agactatcga ccagcccgtt
    21781 agcttttgca tcctgaccgg tccagacgtg gccctgggcg attttatcaa tttgctccgg
    21841 cgtcgaatga cgcgcatcag caaccagcgt gataaagcgt ttatagccat tctcaatgct
    21901 taattgcatc atctgctgcg cttccggcgg cagtgccctg gtgatagaaa catccgccag
    21961 cggtgaagtt gagacaccat cagtatgaac accaatcgaa tccagactat tttctacggt
    22021 ggtgatcacg ccgaagatac cgatagaacc ggtcagggtg ctggggttag ccacaatgta
    22081 attagctggc gtggaaatcc agtaaccacc agatgccgcc atgccgccca tcgatacaac
    22141 cacagactta cccgctgccc gggctgctgc cagttcagcg cgaatcactt cagacgcggt
    22201 aacgctgccg cctgggctat taacacgcag gacaatcgct ttcactttcg ggtcaaggcg
    22261 agcgtcgcgg atttgtgccg cagtggtatc accgccaaca ttcccctgag tttcctcgcc
    22321 atccataatt gcgccattag cgaagacgac accgatgctg tcaccggtat ctgccggcgt
    22381 tttcaatgcg taatcgtaat aactgatggc gcgataattt ttatcagtct tactccagcc
    22441 gaactctttg gtcagagctt tttcgatttc ggcactcgat gccagtgcat cgaccagctt
    22501 gttttccagt gcatatttcg cggtatcgcc accggttttg gttaaaccct caagcaaccc
    22561 ttgcgcgcca gggaatacct gctgagcagg gatctgccgg ttagcggcaa cagtattcag
    22621 atagttttgc cacagctcac caatccagcg gctgtcagct tcgcgggctg ccggtgacat
    22681 atcatcacga ataaacggtt caacggcaga tttatacgta cccacgcgga acacatgggt
    22741 ggaaactttc agcttatcca gcaacgattt gtagtacaga ccgttggtag caaaaccgtg
    22801 cagatcaacc acgccttgcg gtgacagcca aattttattg gcgaaactgg cgagataata
    22861 ttgcccctgg ctgtagttct cgccaacggc ataaaccggt ttcccgctgt cacgaaactc
    22921 tttcagggct ttgccgatgt actgcataga cggctggtcg ccgcctgcga agtttttcag
    22981 atccatcacg ataccggtga tattgcggtc gtccttcgcc tggcgaatgg tgttgacgat
    23041 atcaaacagt gagttttcct gcagacgatc ggaactggca ccaagcagct ggcggcttaa
    23101 tttactaaac cgctgagaac tgtctggttt atcgacgatc acaccagaaa tgtccagcag
    23161 cagtgcgcca cgactggccg tttctttcga atcaccacca ctgacctgca tccagatccc
    23221 cacaccaacc agtacgagga aaataaagaa caggttaagt accatttcac ggacgaaatt
    23281 cagcagacgc cacgtccatt taaaaaatcc ggcaataaat cgccaaaggg ttcgcatgta
    23341 ttctcccaac ttaaggtcac acctgtcaca ggcgcaatag atgtggttat cgtaatgacc
    23401 caaccggcac ttgtcagcag gaattgcgga ctgtgctgta acaaaatcca ctctcgtgtt
    23461 aattttgtga ataaatatca cgacaggagt taatcaaatg gatgcactcg aactattgat
    23521 caatcgccgt agcgcctccc gcttggctga acccgcgcca acgggtgaac aactgcaaaa
    23581 catcctgcgt gcgggtatgc gtgcgccgga ccataagtcc atgcaaccgt ggcatttttt
    23641 tgtgattgaa ggggaagggt gcgagcgttt cagcgccgta ctggaacagg gggcgattgc
    23701 tgccggtagt gatgataaag ctatcgacaa agcccgtaat gcgccgttcc gcgcaccgct
    23761 catcatcacg gtggtggcga aatgcaaaga aaatcataaa gtcccgcgat gggaacagga
    23821 aatgtctgcc ggatgcgcgg tcatggcgat gcaaatggca gccgttgccc agggatttgg
    23881 cggcatctgg cgcagtggcg cattaactga aagtccggta gtgcgtgaag cattcggttg
    23941 ccgtgagcag gataaaattg tcggttttct ctacctcggc acgccgcagt tgaaagcatc
    24001 tacgtcgatt aacgtcccgg acccgacgcc gtttgtaact tatttctgat aattgtcgcg
    24061 aaactgtctg gattgtgagc aaaggcgcag gaattcagac actctcactt atcacttcac
    24121 ggaatgaggg ttaccatacc cggattgcat tgacaggaga tgtccatgag cgagaactcg
    24181 attcgtttga cccaatacag ccacggagct ggttgcggct gtaaaatttc cccaaaagtg
    24241 ttggaaacca tcctgcacag tgagcaggcg aagtttgttg atccgaattt gcttgtgggt
    24301 aatgaaaccc gcgacgatgc ggcggtgtac gatctgggca atggcaccag cgttatcagt
    24361 accaccgact tctttatgcc gatcgttgat aatcctttcg attttggccg cattgcggcg
    24421 actaacgcca tcagcgatat cttcgcgatg gggggcaaac cgattatggc gattgcgatc
    24481 ctcggctggc cgattaacaa actttccccg gaaattgccc gcgaagtaac cgaaggtgga
    24541 cgctatgcat gccgtcaggc gggtattgcg ctggctggcg gtcactccat cgatgcgccg
    24601 gagccgattt ttggtctggc ggtaacgggg atcgtaccga ccgagcgggt gaagaaaaac
    24661 agtaccgcac aagccggatg caaactgttc ctgacgaaac cgctggggat cggcgttctt
    24721 accacggctg agaaaaaatc actgttgaaa ccagaacatc agggactggc gacggaagtg
    24781 atgtgccgga tgaacatcgc aggcgcgtcc tttgccaaca tcgaaggcgt aaaagcgatg
    24841 accgacgtta cgggctttgg tctgctgggc cacttgagcg aaatgtgtca gggggctggt
    24901 gtgcaggcac gcgtcgacta tgaagcgatc ccgaaactcc ccggtgttga agagtacatt
    24961 aagttgggcg cagtacctgg cggcactgaa cgtaactttg ccagctacgg tcatctgatg
    25021 ggtgaaatgc cgcgtgaagt gcgcgatctg ctgtgtgatc cgcaaacttc tggcggtttg
    25081 ctgctggcgg tcatgccgga agcagaaaat gaggttaaaa ctacagccgc cgagtttggc
    25141 attgaactga cggcgattgg cgaactggtg ccagcgcgcg gcggtcgtgc catggttgag
    25201 atccgttaat tcaatgcggt tgtttattgc cgaaaaaccg agtctggcgc gcgccattgc
    25261 tgatgtcctg cccaaaccgc accggaaagg cgatggcttt atcgagtgcg gtaatggtca
    25321 ggtggtgact tggtgtatcg gtcacctgct tgagcaggcg cagccagacg cctacgacag
    25381 ccgctatgcg cgctggaatc ttgcggattt gccgattgtc ccggaaaagt ggcaattaca
    25441 gccacgaccc tccgtgacca aacaacttaa cgtcatcaaa cgcttcctgc atgaagccag
    25501 cgaaatcgtt cacgccgggg acccggatcg tgaagggcaa ttgctggtgg atgaagtgct
    25561 ggactatctg caactggcac cggaaaagcg ccagcaggtg cagcgttgct tgataaacga
    25621 cctgaacccg caggcggttg agcgggcgat cgaccgtctt cgttccaaca gtgagtttgt
    25681 gccattgtgc gtttctgcgc tggcgcgagc gcgtgccgac tggctgtacg gcatcaatat
    25741 gacccgtgcg tataccattc tcggtcgcaa tgccggttat cagggcgtac tttccgtggg
    25801 acgcgtgcag acacccgtgc tcgggctggt ggtgcgccgc gatgaagaga ttgaaaactt
    25861 cgtggcgaaa gacttctttg aagtcaaagc acatatcgtg acacctgccg atgagcggtt
    25921 taccgctatc tggcaaccga gcgaagcgtg tgaaccgtac caggatgaag aagggcgctt
    25981 gttacatcgt ccactggcgg agcatgtggt taaccgcatt agtggtcaac cggctattgt
    26041 caccagctat aacgataaac gggaatcaga atccgcgccg ctgccgtttt cgctttcggc
    26101 gttgcagatt gaagcggcaa aacgcttcgg tctgagcgcg cagaacgtgc ttgatatctg
    26161 ccagaagctg tacgaaacgc acaagctaat cacttatccg cgttccgatt gtcgctattt
    26221 gccagaagaa cattttgccg gacgccacgc ggtgatgaat gccatcagcg ttcatgcacc
    26281 ggatctgttg ccgcagccag tggtagatcc agatatacgc aaccgctgtt gggatgacaa
    26341 aaaggtcgat gcgcaccacg ccatcatccc gaccgcacgg agttctgcga tcaacctgac
    26401 ggagaacgaa gcgaaggtct ataacctgat tgcccgtcaa tacctgatgc agttctgccc
    26461 ggatgcggtg ttccgcaagt gtgttatcga actggacatt gccaaaggca aatttgtcgc
    26521 taaagcccgt tttcttgctg aagcaggctg gcgcacgctg ttaggcagca aagagcgcga
    26581 tgaagaaaac gacggcacgc cattgcctgt ggtggcgaaa ggcgatgagt tgctgtgtga
    26641 aaaaggtgaa gtggtagagc ggcaaaccca gccgccgcgc cattttaccg atgcaacact
    26701 gctttcggcg atgaccggga tcgcgcgctt tgtgcaggac aaagatctga aaaaggtcct
    26761 tcgtgcgacc gatggtctgg ggacagaagc aacccgtgcc gggattattg aactgctgtt
    26821 caagcgtggt tttctgacca aaaaagggcg ctatatccac tccaccgacg ccggaaaagc
    26881 gttattccat tcgctgccag aaatggcgac gcgaccggac atgaccgcgc actgggaatc
    26941 ggtgctgacg caaatcagcg aaaagcagtg tcgctatcag gactttatgc agccgctggt
    27001 ggggacgcta tatcagctta ttgatcaagc caaacgtacg ccggtgcggc agtttcgcgg
    27061 cattgtggct ccgggcagtg gtggcagtgc tgataagaaa aaggctgcac cgcgtaaacg
    27121 tagtgcgaaa aaaagtccgc cagcagatga agccggaagc ggggcgatag cgtaagcgag
    27181 cgaatctttc gtgctattcg agtcatattc tgaaatatcc agcggatcaa gaaaattcgt
    27241 tggatatttt tttgcatgga taaaattatc gcctctaaag tatgtaataa cagggaatgt
    27301 gatgaaaaaa gttttattac aaaaccatcc tgggagcgag aagtattctt ttaatggctg
    27361 gggaatattt aatagtaatt ttgaacgggt gattaaagaa aataaggcca tgctgctttg
    27421 taagtggggg ttttatttaa catgtgttgt cgctgtaatg tttgtattcg cagcgataac
    27481 atccaacggt ttgaatgaaa gaggcctgat taccgcggga tgctcttttc tttatctatt
    27541 aattatgatg gggcttattg ttcgggccgg ttttaaagca aaaaaagaac aactacatta
    27601 ttatcaggct aaaggtattg agccgctcag tatcgagaag ttacaggcgc tacaattgat
    27661 cgcaccttat cgattctatc ataagcaatg gtctgaaacg ctagagttct ggccgcgaaa
    27721 gcctgaacct ggcaaagata ccttccaata tcatgtgctt ccttttgact cgatcgatat
    27781 catcagtaaa agacgcgagt ctttagagaa tcaattgggt atcgaagata gcgaaagtta
    27841 ttgtgcctta atggagcatt ttctttctgg cgaccatgga gccaatacct ttaaagcaaa
    27901 catggaggaa gccccagagc aggttatcgc cgtgttgaat aaatttgctg tttttccctc
    27961 agactatatc tctgattgcg ctaataatcg ctccggtaaa tcctcggcga agctaatatg
    28021 ggcggcggaa ttatcatgga tgatctcgat atcaagcaca gcttttcaaa acgggacaat
    28081 tgaagaagaa ctggcctggc attatataat gcttgcttct cgaaaggcgc acgagttgtt
    28141 cgaaagcgaa gaagattatc aaaaaaatag tctaatggga tttctttact ggcatatctg
    28201 ctgctatcgc agaaagttaa cggatgctga acttgaagcg tgttatcgtt acgacaagca
    28261 gttttgggag cactacagta agaaatgccg ttggcccata agaaatgttc cctggggagc
    28321 atcatccgtt aaatactcat aaacgcctga aattttgccg ggggcgctgc gcttgcccgg
    28381 cctacattag agcgtaactg caatgaattg tgcccatctg taggcctgat aagcgtagcg
    28441 ccatcaggca tttacaactt aaatcacacc ctgcgccagc atcgcatcgg caaccttcac
    28501 aaaaccggca atgttcgcgc cctgcacgta gttggtttgc tcaccttcac caccatgctc
    28561 aacacaggca tggtggatat ccagcatgat gtgatgcaaa cgtgcgtcaa ctttctcggc
    28621 tttccagccc aggcgcgcag cgttttgtgc catttccagg cccgacgttg ctacaccgcc
    28681 agcattagcc gctttacccg gtgcaaacag tacgcctgcc tgctggaaca gttcagtcgc
    28741 ttcgatggtg gtcggcatat ttgccccttc agcgacggct ttaacgccat tggcgataag
    28801 ctgatgcgcg gtgtcaacat ccagttcgtt ctgggtggcg caaggcaggg cgatatcaac
    28861 cggtacagac cacggctgtt ggccttcgag atagaccaga ccaaattctt tggcgtaatc
    28921 tgccactcga ccatcgcggc tggctttgat ttcgataaga cgtgccagtt tctctttcgt
    28981 gaatccgctt tcatcaacta cagtgccgct ggagtctgac gcagtgatca cacgagcacc
    29041 aaattccatc gctttttcga tagcgtactg ggcgacgttg ccggagccag aaacggaaac
    29101 gcgcatccct tcaaaaccca taccgtggcg ttttaacatt gcttctgtga aataaaccag
    29161 accgtagccg gtagcttccg ggcgaataag actaccgcca aatgaaaggc ccttaccggt
    29221 gaagacgcag gcggtattgt tggagagctt tttcatcatc cccgccataa agccgacttc
    29281 acgaccacca acgccgatat cacctgccgg aacgtcggta tccgcgccca ggtggcgata
    29341 cagttcagtc atcagcgcct ggcaaaaacg catcacttta ccttcgcttt ttcctttcgg
    29401 atcgaaatcg ctgccgcctt taccaccgcc catcggcagg gtagtcaggg catttttgaa
    29461 ggtttgttca aagccgagga atttgagaat ggaaaggtta actgacggat ggaagcgcat
    29521 accgcctttg tacgggccga tggcagagct gaactgcaca cgccatgcac ggttgacctg
    29581 tatctggttg cgatcatcaa cccataccac gcgaaactgg atcacgcgct ccggttcaac
    29641 cagacgctcc agtaatgaca tctggcgata ttttggattt tgttcaagaa aaggccagag
    29701 tgtggtcatt acttcacgaa cggcttgcgc gaactcggtt tgattcgggt cgcgcttttg
    29761 gacatggttg aggaatgact ccagagaata tgtctgatcc atagatataa aaaccttata
    29821 tattaatacg attgtgctta tgttgtttat gtcatgtgct tttgcagttt tcgactatac
    29881 catcaagaat gtgacggtaa gcaagaaaaa aatgacccag gaaagcaaat taataacgag
    29941 agtaatctca taaccaaatg cgataactca gacgctaaag ttttgccacc cggctaccgt
    30001 tatcagtagc agagacaata acgttaagga gttccctgtg agtcgagcat tgttcgccgt
    30061 agtattagca tttccattaa ttgcactggc taacccgcat tatcgtccgg acgtggaggt
    30121 aaacgtaccg ccagaagtgt tcagttcagg cgggcagtca gcgcagccct gtactcagtg
    30181 ctgcgtttat caggatcaaa attattctga aggggcggta attaaagccg aaggaatact
    30241 gctgcaatgc cagcgtgatg acaaaaccct tagcactaat ccgctgctct ggcggcgcgt
    30301 aaagccataa acgcctctaa taatggaatg tcagcagggg ccagcggata ttgcagcgcc
    30361 tcttcaggtg agcaccagac cagcgcctga tgttcatgtg cctgtaacgt cccgtggaag
    30421 tcgggtacgt gccaggcatg aagatggata atccggcccg aaacttctcg ctgatggctg
    30481 gcaacatact cacccacagt tgcttcgatg tccaattctt cgtttaactc acgcaccagc
    30541 gcctgctgct ggctttcatc cagctcgact ttacctccgg caaactccca taatcccgcc
    30601 tgatcgctct gggcggggcg ttgcgcgagt aaaattttgc catcacgttc aatgatggcg
    30661 gcaacaactt caatcatttt cataaattac acacgggcag ataagcgaaa aatgccataa
    30721 tatccgcact gttgctcaat cagaaaggct ttatatttaa ggagtgcctg tgctagaccg
    30781 ccatcttcat ccccggatta aaccgttgtt gcatcagtgc gtgcgggttc ttgataaatc
    30841 gggcattacg ccagatggtt taacattagt gggatttgcc atcggcgtgc tggctctgcc
    30901 gtttctggcg ctgggctggt atctggcggc gctagtcgtc attttgttga acaggctgct
    30961 tgatggtctg gacggtgcgc tggcgcggcg cagagggctt accgacgcgg gcggctttct
    31021 tgatatttct ctcgattttc tcttttacgc gctggtgccg tttggcttta ttcttgctgc
    31081 accggagcaa aatgcgctgg caggcggctg gttgttgttt gcgtttatcg gcacgggcag
    31141 cagttttctc gcctttgctg cactggcagc aaaacatcag attgataacc ccggttatgc
    31201 acataagtcg ttttactatc tggggggatt aaccgaaggc acagaaacga tcttactgtt
    31261 tgtgctggga tgtttattcc ctgcatggtt tgcgtggttt gcgtggtttg cgtggtttgc
    31321 gtggtttgcg tggatcttcg gtgcgttgtg ctggatgacg acctttacgc gggtgtggag
    31381 tgggtatctg acgctgaagt cgctccagcg tcagtaattg caatgttatt tgctactgcc
    31441 cgggccgcgt tcaccggttg caaccggatt ttttggatcg ctgctccatt cgtaccagcc
    31501 gccgtcatac acggaaacat tcttccaacc catggcgcgt gcgtacataa aggtttcgga
    31561 cgcgcgccag ccggtgccgc agtagaatga aacttgctga tctggtttga tattccatgc
    31621 tttccacata gcggtaatat catcggcgct acgcatggtg ccatccgggt tatggaaatc
    31681 ttccatatgc gtcgagtcgc taccagcgtg tccccaacgt gctccggcta tttcaccttt
    31741 tggtttaata tagctgtaac cgctggtcgt accgataaat tctggccacg aacgaatgct
    31801 caccagcgat gcatcctggc gatgcagcag tccacgggcc tgttccatat cgagcatcaa
    31861 ctgcggttgt gccgggatct tcacgccgaa atctggttcc gctttcactt tcggtggcgt
    31921 tcctcgctca acaggcagtc ccgcgtcgga ccaggtttgc cagccgccat ccagcagacg
    31981 cacatctttc acgcctgcgt agagcataat ttgcgccaca cgcgctccag cgtatacgtc
    32041 acgcccgtac agaatgaccg tggtgtcatg gcgaatgccg tgttttgcca gcatcgcttt
    32101 tagttgttca tcagaaactt tgttccacag cggttcgctt tccacttcgt tggtatcgat
    32161 gtagtcagcg ccaggaatat ggctgataag gtaaagctta ggagcgcccc aggccgcttc
    32221 aatgactttc cagccaccga caggtttcgc cgtaacctct ttaccttgtt gcaggtcatg
    32281 cagccattgc ggataaacca gctgctcaaa gtgcggcagt ttttgtagac gggaaggttc
    32341 gcttagcgcg tcactcagaa tggagatatg cgtaaaacct gctttttgca gtcgcgtttt
    32401 gacggcgtcg acatctttgc cattaccgta cagcgccacc ggggcatcgg ctttcaggtt
    32461 atgttgcttg atccacgcgt tgagctgttc ggtgctcatt ttgtcgagcc agctagcaga
    32521 gaggtttaag gcggcaggtt catgaccaga agggccattt aaggtttgtg gccagccgtt
    32581 ataaaacgcg ctggggcgag tatctatcgc tttgccattt tgttgctgaa gttggtcaag
    32641 tgtaagaggc ttcgccagtt cagcggccca cgaagaagcg caagccagcc ctaaagccat
    32701 tgccagcgcg gtcatttgag aaacacgttt catcgaaaat cccgacatta tagttttgag
    32761 tgcgccattc tgaactgacg cattgagaaa aactttgcgt tatcgcagtt tgttgtaatt
    32821 ttctgaccac tgcgccatat ccagaacaga actatcagca ggaacatcct ggagatcgtg
    32881 cgttacctga acgacgggga tcgccagggc gcgaacttcg ctgaacaccc actggcgaaa
    32941 attatcgcgc agagccacat caagacggct gaatggctca tccaggagta acgcttttgg
    33001 ctgggcgaga agggcgcgta gtagagcaac gcgcgctcgc tgaccgccag acaaagtggc
    33061 aggatcctga tggaaagctc cgtcaaggcc tgaacgctca agtgcatcat tcacggcatt
    33121 acgtcgggca ttccccttaa gtgtcgccgg tagcgccagc agtaaatttt gcccgacgct
    33181 gaactggtca aataacagtg catcctgaaa aagaatgcca atctgacgct gtgcggtggg
    33241 tagcatgtca atccgttgct cattgagcca tagctcacct gtacaagaaa actgtccggc
    33301 cagtgcacca atcatccatg aaaacagagt ggattttcca cagccagatg gccccattaa
    33361 cgtgacgatg tcacctttat caaccgtaaa gttaacgttt gtcagcaagc ggctttctgg
    33421 taaacgtagc gaaacatttt tcacgcagag cattagcgga ttccttgtct gacataacct
    33481 acccattttg cgactaacgc ggtcagggca aaaataataa gcggtaacaa cagttgccat
    33541 aaagcctggg cggcgagaat accgttgctg ccgccgctgc ttaatgccac cgcctcagtg
    33601 gtgagcgtcg gaaaacgccc cgcgcccagc cacagcgtcg gcatatactg cgcaatactg
    33661 actgaaaatc ccaccgcgaa ggcaatcagc acagggcgca acatgagtgg gcatttcacg
    33721 taaaagaaga ttttggcccg cgaccagccc agtgtttgcg caatcaaaat taaccgtgaa
    33781 tcaatgcgct gccaggcggg ttgcaggata aacagcatcc acggcattac ccacagcaga
    33841 tgcccccaga ccaccgccgt ccagcttcca tccaggttca gccatagcgc cagcgtgtac
    33901 tagcccgcca ccagtggcag agcgggcaat agaatgggca accatagcca caactggcga
    33961 cgctgtgccc cccattccag ccacagcagt aacaggagca aagcgatgaa tgttgctacc
    34021 agccccattg tcagactgtt gatgagtgct tcactattga tcgtcgactg atccgcgagg
    34081 atcgccagca gaaccacaca cagcacaccg gttaagggta aaaaactcgc cagcgtagtg
    34141 cctggtaata aaggtgtggc aggcttgcga atgccatcta cgcggggaat agtgcgccgc
    34201 cagctgcgcc atagcagata gctcagcaaa acgtaggcgg cgagtaacag catcagcagc
    34261 agactggcga gtgcgccttt cgtttgttga tccgcgtcgc cctgagttaa ccactgccag
    34321 ctaattactg ccagcgtcgg tggattacct ggcccaagaa taatcgccac atccacgacc
    34381 gaaagcgacc aggcaacaat cgccagcatc gccattgcca gcgaaggcgc gacggagggc
    34441 aacagcagcc agttcaggca ttgccagcgg ctgtagccca gcgaatccag cacaatgacc
    34501 tgctgcaaca gccgtttttc gctcaatact gccactaaga tccacagcag aaatgcgctt
    34561 tctttcactg caagggtgag gcccagaccg atccaaatcg gtccattggc ggagtgaaat
    34621 acgggaaata gtcatatagc agccctccgt cagcaaagag cagcagggcg ctggtggcaa
    34681 aagccacatg gggaatggcg agcagccacg gcagacgggc gcacatacgc tgccatttcg
    34741 gcccaggcca tagcgccaca atcaccagca gggcaatcaa caatgccccg accgccgcga
    34801 tggttgtcga caccagcgtt gccagtaatg cctgcggtaa ctgtggatcg gcaaataacg
    34861 cctgccagcg tgtcaacgac aacgcaggcg atatcagcgt gagagctgcc gggatcagcg
    34921 gtgcataaat caccgccacc atcgcccaca gcaaaaagat taatgcgtac cgtaacggcg
    34981 taaccattct tgttccagtg cattcaccca tcctgcgtgc ggttcagcca gtaccggcgg
    35041 cagatcctgc ggcattcttg attgcaatat ttcgcgctgc ccgtcaggca gtttttgcgg
    35101 atcgagaaca gaaggatcgc cccagacaac gggatctgct ttacgcagtt gcgcatcggg
    35161 tgagagcagg aaattggcaa ctaccttcgc cgcagcactg gcattcgcgt tggctggaat
    35221 ggtgacgaaa tgcacgttgc caatcatccc ctcgcgaaag ccaaaactgt aactgcttgc
    35281 cggcaaatcg ccgctggcga ttttttgctg cgcatgcgcg gggttaaagg tcagcgacag
    35341 gcgcaatgtg ccggctttca gcagagcatc catccgcgcg ggcgaaggcg ggaaatcctt
    35401 tccttcgcgc cacaaatacg gatgcagtgc atcaagatat tgccacaagg gcgcagtgac
    35461 acgggcgaaa gtcgcatcgt ccggcgcttc ttttaatgcg gcgggatctg gcgtcagcat
    35521 aatcagcaac tgttcaagaa acgccgtgcc ggtaaagtcc ggtgggcgcg gataagtaac
    35581 cgtgccggga ttggctttag caaactccag taaggcttgc ggcgtttgtg gtggctgtgg
    35641 cgtaacatcg cggcgggcga taaacgtcag ttgtgcgccg ccccagggcg attccgcacc
    35701 ttgtgtcggc actgaaaaat cttcccgcac cggcagctgt gtgtcgacat agcgccagtt
    35761 gggcagagtc tccgcccagc ccgtttgtaa taaattggcc tctttta
//
LOCUS       DADLTF010000023        35305 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.023, whole genome shotgun sequence.
ACCESSION   DADLTF010000023 DADLTF010000000
VERSION     DADLTF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.023"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1165)
                     /gene="setB"
                     /locus_tag="JN749_001102"
     CDS             complement(<1..1165)
                     /gene="setB"
                     /locus_tag="JN749_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HAY7367588.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EIHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVM"
     gene            1533..2663
                     /locus_tag="JN749_001103"
     CDS             1533..2663
                     /locus_tag="JN749_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HAY7367589.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNCIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2663..3601
                     /gene="fruK"
                     /locus_tag="JN749_001104"
     CDS             2663..3601
                     /gene="fruK"
                     /locus_tag="JN749_001104"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAY7367590.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3618..5309
                     /gene="fruA"
                     /locus_tag="JN749_001105"
     CDS             3618..5309
                     /gene="fruA"
                     /locus_tag="JN749_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HAY7367591.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5733..6674
                     /locus_tag="JN749_001106"
     CDS             5733..6674
                     /locus_tag="JN749_001106"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HAY7367592.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYVDSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSASKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6662..7600
                     /locus_tag="JN749_001107"
     CDS             6662..7600
                     /locus_tag="JN749_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HAY7367593.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQIAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7694..8944
                     /locus_tag="JN749_001108"
     CDS             7694..8944
                     /locus_tag="JN749_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7367594.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9015..9713)
                     /gene="yeiL"
                     /locus_tag="JN749_001109"
     CDS             complement(9015..9713)
                     /gene="yeiL"
                     /locus_tag="JN749_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HAY7367595.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            9844..10785
                     /gene="rihB"
                     /locus_tag="JN749_001110"
     CDS             9844..10785
                     /gene="rihB"
                     /locus_tag="JN749_001110"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HAY7367596.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10885..12135
                     /locus_tag="JN749_001111"
     CDS             10885..12135
                     /locus_tag="JN749_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAY7367597.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLVLNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12191..13279)
                     /locus_tag="JN749_001112"
     CDS             complement(12191..13279)
                     /locus_tag="JN749_001112"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7367598.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLVDCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13282..14139)
                     /gene="nfo"
                     /locus_tag="JN749_001113"
     CDS             complement(13282..14139)
                     /gene="nfo"
                     /locus_tag="JN749_001113"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAY7367599.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14213..15262)
                     /locus_tag="JN749_001114"
     CDS             complement(14213..15262)
                     /locus_tag="JN749_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HAY7367600.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15361..16242
                     /locus_tag="JN749_001115"
     CDS             15361..16242
                     /locus_tag="JN749_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7367601.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16448..17917
                     /gene="lysP"
                     /locus_tag="JN749_001116"
     CDS             16448..17917
                     /gene="lysP"
                     /locus_tag="JN749_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HAY7367602.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18209..20200
                     /gene="cirA"
                     /locus_tag="JN749_001117"
                     /pseudo
     CDS             18209..20200
                     /gene="cirA"
                     /locus_tag="JN749_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20232..21068)
                     /locus_tag="JN749_001118"
     CDS             complement(20232..21068)
                     /locus_tag="JN749_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HAY7367603.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21326..21994
                     /gene="folE"
                     /locus_tag="JN749_001119"
     CDS             21326..21994
                     /gene="folE"
                     /locus_tag="JN749_001119"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HAY7367604.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22011..23168
                     /locus_tag="JN749_001120"
     CDS             22011..23168
                     /locus_tag="JN749_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HAY7367605.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23310..24278
                     /gene="galS"
                     /locus_tag="JN749_001121"
     CDS             23310..24278
                     /gene="galS"
                     /locus_tag="JN749_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HAY7367606.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRAATVLCRR"
     gene            24629..25627
                     /gene="mglB"
                     /locus_tag="JN749_001122"
     CDS             24629..25627
                     /gene="mglB"
                     /locus_tag="JN749_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HAY7367607.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25688..27208
                     /gene="mglA"
                     /locus_tag="JN749_001123"
     CDS             25688..27208
                     /gene="mglA"
                     /locus_tag="JN749_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HAY7367608.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27224..28234
                     /gene="mglC"
                     /locus_tag="JN749_001124"
     CDS             27224..28234
                     /gene="mglC"
                     /locus_tag="JN749_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HAY7367609.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28305..29540)
                     /gene="preA"
                     /locus_tag="JN749_001125"
     CDS             complement(28305..29540)
                     /gene="preA"
                     /locus_tag="JN749_001125"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HAY7367610.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29534..30774)
                     /locus_tag="JN749_001126"
                     /pseudo
     CDS             complement(29534..30774)
                     /locus_tag="JN749_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(30968..31207)
                     /locus_tag="JN749_001127"
     CDS             complement(30968..31207)
                     /locus_tag="JN749_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HAY7367611.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31210..31929)
                     /gene="sanA"
                     /locus_tag="JN749_001128"
     CDS             complement(31210..31929)
                     /gene="sanA"
                     /locus_tag="JN749_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HAY7367612.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32079..32963)
                     /gene="cdd"
                     /locus_tag="JN749_001129"
     CDS             complement(32079..32963)
                     /gene="cdd"
                     /locus_tag="JN749_001129"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAY7367613.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33093..33788)
                     /locus_tag="JN749_001130"
     CDS             complement(33093..33788)
                     /locus_tag="JN749_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HAY7367614.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33785..34183)
                     /locus_tag="JN749_001131"
     CDS             complement(33785..34183)
                     /locus_tag="JN749_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAY7367615.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34422..>35305
                     /gene="dusC"
                     /locus_tag="JN749_001132"
     CDS             34422..>35305
                     /gene="dusC"
                     /locus_tag="JN749_001132"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HAY7367616.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8670 a         8629 c         9322 g         8684 t
ORIGIN      
        1 tcattaccca aaacagagtg gcgataatca tcaccatcgc aaaccagaac acagcgtgat
       61 aattccagat ctcggcgacg atgcccgcca ctgatcctgc gatgatccag cccacgcgcg
      121 acgtgttggt atagagcgtg gtggctgaac ctgcctgacc gggcatcaaa tcctgaaaat
      181 agagcatccc gatgccgccc agaatgccaa taaaaatagc atttagcagc tgcaagccca
      241 acagaatggc aggtgaatgc gccatcagca ttcctgcgta aaaacagacg ccacccacgg
      301 cagcaacgcg cattaagaaa cgcttaccca gacgtttggc gaaatatccg gcaatcaaca
      361 tcgtcgggat ttccagcccg gcggcggtcc ccatcatcac accggccagt ttctcgggaa
      421 gatgcagttc gttgataata aatagcggca tgttgatgat gtacaggctg ttcgagcccc
      481 acatcaatgt acaaatgaca aacagcagta gcgtatcgcg acggttacgg cgcggcgctt
      541 caaccgtgcc ggtcgccagc ggaagctctt tttgcatcga cggtaaaaac agccacacca
      601 tcacgccgca aacaataaac gctaccgctg cgctcagata cattaccgta aagctgaaac
      661 ccatcgctaa cgcataagcc agcggtgggc caatgaccca tgccagtgaa acctgagcgc
      721 gtaaaaaaga gctgaacatc accgcctcac gtccggtttt gtcggcatgt tcacgggcaa
      781 gggcaaacat ttgcgggtta gcggtcgagc caaagctgct aagaaagacg ccaacgaata
      841 gcaaaacaaa gtagttgcga ttccaggcaa aaagggtgca ggccagcacg cctaacaggc
      901 agcaaaagac aatcagcgat ttgcgatcgc cgcgcttatc agagcgccca gcgagaaact
      961 gacttaccag aatcccaatg acagcgctgc cggtaaagaa gaatcccacc atcgccggac
     1021 gggcatgtat ttcatcggta aggaaaatac tgagtgtcgg ggtttgcaga gcacccgcaa
     1081 taccggtgag aaaggcaacg attaaaaacg ccgtcgaggt caggtcaaac gatttcgcgc
     1141 tggagactgc gggggagtta tgcatgctgg tcgatcactg gaagaggaag acgcggagtt
     1201 tacgctgctt gtcagaaaat aaacagacag cgaaatcaaa agcgccacga ataatcaaaa
     1261 tgccatttca ctgttgctga ttggctgctg acgctgctga aatgaaattg ctgataacgg
     1321 attttcccat cagcaattag gaaaaatgac aaaaaattgt gcagcacatc aaacttttgc
     1381 tcataacttt acggctttcc ttgcgtgctg aaacagaagt attatgcttt cttgaaacgt
     1441 ttcagcgcga tcttgtcttt aaccctaagc caggttggcg ctttttttct catagaggct
     1501 gaatcgtttc aattcagcaa gagaggagaa ctatgttcca gttatccgta caggacatcc
     1561 atccgggcga aaaggccgga gacaaagaag aggcgattcg ccaggtcgct gcggcgctgg
     1621 tgcaggccgg taatgtagca gaaggctacg tcaatggcat gctggcgcgc gaacagcaaa
     1681 cctcaacgtt cctcggcaat tgtattgcta ttccacacgg cactaccgac acccgcgatc
     1741 aggtgctgaa aaccggcgtt caggtatttc agttcccgga aggcgtcacc tggggtgacg
     1801 gtcaggtagc gtacgtggca atcggtattg ctgccagctc ggataaacat ctgggcctgc
     1861 tacgccagct gacccacgta ctgagcgatg attccgttgc tgaacaactg aagtcagcaa
     1921 caacagcaga agaacttcgc gcattactga tgggcgaaaa gcagagtgag cagctgaagc
     1981 tcgacaacga aatgctgacg ctggatatcg tcgccagcga tctgctgact cttcaggcgc
     2041 tgaacgctgc gcgtctgaaa gaggcgaggg cagttgacgc cactttcgtc accaaagcca
     2101 tcaatgaaca accgctgaat ctcggacagg gtatctggct gagcgatagc gccgaaggca
     2161 atctgcgtag tgcgattgcg gtaagccgtg cggcaaatgc ttttgatgtg gacggcgaaa
     2221 cggcagccat gctggtgagt gtggcgatga atgacgatca gcccatcgcg gttcttaagc
     2281 gtctcgctga tttgttgctc gacaataaag ctgaccgctt gctgaaagcg gatgcggcaa
     2341 cgttgctggc gctgctgacc agcgatgatg cgccgaccga cgacgtgtta agcgcggagt
     2401 ttgtggtgcg caatgaacac ggcctgcatg ctcgtccagg taccatgctg gtcaatacca
     2461 ttaaacaatt taacagtgat attaccgtga caaaccttga tggcaccggc aaaccggcaa
     2521 acggacgtag tctgatgaaa gttgtggcac ttggcgttaa gaaaggtcat cgcctacgct
     2581 ttaccgccca gggtgcagat gctgaacagg cgctgaaagc cattggcgac gctatcgctg
     2641 ctggtcttgg ggagggcgca taatgagcag acgtgttgct acgatcaccc ttaatccggc
     2701 ttatgacctt gttggtttct gcccggaaat tgaacgcggc gaagtgaacc tggtgaaaac
     2761 caccggtctg catgcggcgg gtaaaggcat caacgtggcc aaagtattaa aagacctggg
     2821 aattgatgtc accgttggcg gcttcctggg taaagacaat caggatggtt ttcagcaact
     2881 gttcagcgag ctgggcattg ccaaccgttt ccaggttgta caggggcgca cccgaattaa
     2941 cgttaagctg acggaaaaag acggcgaagt gaccgacttc aacttctcgg gttttgaagt
     3001 cacccccgcc gactgggaac gctttgtgac tgattctctg agctggctcg gtcagttcga
     3061 tatggtctgt gtcagcggaa gcttaccgtc aggcgtcagc ccggaagcgt tcaccgactg
     3121 gatgactcgc ctgcgtagtc agtgtccttg cattatcttt gatagtagcc gtgaagcgtt
     3181 agtagcaggt ttgaaagcgg caccgtggct ggtgaaacct aaccgccgcg agttggaaat
     3241 ctgggcaggc cgtaaactgc ctgaaatgaa agatgtgatt gaagctgcac atgcgctacg
     3301 tgaacaaggt attgcgcatg ttgttatttc actgggtgcc gaaggcgcgc tttgggttaa
     3361 tgcctccggc gaatggatcg ctaaaccacc gtcagtcgat gtcgtaagca ccgttggcgc
     3421 aggggattct atggttggtg gcctgattta tggcttgctg atgcgtgaat ccagtgaaca
     3481 cacactgcgt ctggcgacag ctgttgcagc cctggcggta agtcaaagca atgtgggtat
     3541 taccgatcgt ccgcagttgg ccgcaatgat ggcgcgcgtc gacttacaac cttttaactg
     3601 acagcaggag aggcataatg aaaacgctgc tgattattga cgctaatctc ggtcaggcac
     3661 gcgcctatat ggcgaagacc ctgctgggcg cggcggcgcg aaaagcaaaa ctggaaatca
     3721 tcgacaatcc gaacgacgca gaaatggcga ttgttctcgg tgattccatc ccgaatgaca
     3781 gcgcgctgaa cggtaaaaat gtctggctgg gtgatatttc ccgggcagtt gcgcaccctg
     3841 agctgttcct gagtgaagcc aaaggccatg cgaaacctta cactgcgccg gtcactgcga
     3901 cagcaccggt tgccgccagc ggtccgaaac gcgtagttgc ggtgactgct tgcccgactg
     3961 gcgtagcaca cacctttatg gcggctgaag ccattgaaac cgaagcgaaa aaacgtggct
     4021 ggtgggtgaa agttgaaacc cgtggttctg ttggcgcggg taatgcaatc actcccgagg
     4081 aagtagccgc agcggatctg gtgattgtgg cggcagatat cgaagtggat ctggcgaaat
     4141 ttgctggtaa accgatgtat cgtacctcta ccggtctggc gctgaagaaa acctcgcagg
     4201 aactggataa agcggttgct gaagcaacgc cgtatgaacc ggcgggcaaa gctcaaacgg
     4261 cgaccactga aggtaagaaa gagagtgcag gcgcttatcg tcacttgcta acgggcgtct
     4321 cttacatgct gccgatggtc gttgctggtg gtctgtgtat cgcgctttct tttgcttttg
     4381 gtatcgaagc gtttaaagag ccgggtacgt tggcagcggc gctgatgcag attggtggtg
     4441 gttcagcctt tgcgctgatg gtgccggtac tggcaggtta tattgccttc tccattgccg
     4501 atcgtccggg cctcacgccg ggtctgattg gcggtatgct ggcggtcagc accggttctg
     4561 gcttcattgg cggtattatt gcgggcttcc tggctggtta cattgcgaag ttaatcagta
     4621 cgcaactgaa actgccgcag agtatggagg cgctgaaacc gatcctgatc attccgctaa
     4681 tttccagtct ggtggtcggt ctggcgatga tctacctgat cggtaaacca gttgctggca
     4741 ttctcgaagg gctgactcac tggctgcaga ccatggggac tgcgaatgcg gttctgctgg
     4801 gggcgatcct cggtggcatg atgtgtactg acatgggcgg tccggtaaac aaagcagcgt
     4861 acgcattcgg tgtgggtctg ctgagtactc aaacctatgg cccgatggcg gcgattatgg
     4921 cggcaggtat ggtgccaccg ctggcaatgg gcctggcaac aatggtggcg cgtcgcaaat
     4981 tcgacaaagc gcagcaggaa ggtggcaaag ccgctctggt attggggctg tgcttcattt
     5041 cggaaggtgc aattccgttt gctgctcgtg atccgatgcg tgtgctgccg tgctgtatcg
     5101 tgggtggggc gctgactggc gcaatctcaa tggcgattgg tgcgaaactg atggcaccgc
     5161 acggtggtct gtttgttctg ctgatccctg gcgctattac gccggtattg ggttacctgg
     5221 tagcaattat tgccggtacg ctggtggcgg gtttggccta tgccttcctg aaacgtccgg
     5281 aagtggacgc agtagcgaaa gcagcgtaat aaaaggtgtg ttacagggca gaaatttcct
     5341 gccctgtaac atttgatgag cgcaaagaga atgaccagct tgtgaatgta attttatgaa
     5401 tttaaatggt tttatctttc agtagaaact tcaaacgttc atcaaacact aaggttattt
     5461 cctcaagggc agattatttt ctgccctttt ttagtgttcc tgttctgtgt atcccgtcac
     5521 aaattcctcg cttttgtaat tcaggcattt aatcgcattt tgtgagcctg ctcaaatctg
     5581 cccattttgc ctctctggcg gtaatttact ttacgtaaaa catgcatact atgagcacat
     5641 gtttaaatat gaacacatgt ttaaaccgtc tttgcagtat ctatattctt gtggattgac
     5701 agggttggta attttttacc agaggacgtg ctatgcgcga aaaggattat gtcgtaatta
     5761 taggttcggc gaatattgat gtcgccggat attcacatga atcattaaat tatgtggatt
     5821 caaatccagg taaaataaaa tttacgcctg gtggagtagg gcgcaatatt gcacaaaacc
     5881 tggcgttgct gggtaacaaa gcctggctac tgagcgccgt aggcagtgat ttttatggtc
     5941 aatcgctgct aacgcaaacc aatcaatctg gtgtttatgt cgataaatgc ctgattgtgc
     6001 cgggagaaaa tacgtcgagt tatttatcat tactcgataa taccggtgaa atgctggttg
     6061 ctataaatga catgaatatt agcaacgcta ttacagctga atatctcgca cagcaccgtg
     6121 aatttattca gagggcaaag gtcattgtcg cggactgtaa tatcagtgaa gaggcactgg
     6181 catggattct ggataatgcc gccaacgtac ccgtatttgt cgatccggtt tcagcgtcga
     6241 aatgtgtcaa agtgcgcgac cgtctaaatc agatccacac tctgaagcca aaccgccttg
     6301 aagcggaaac cctgagtggg attgcgctgt cagggcgtga cgatgtggca aaagttgctg
     6361 cctggttcca tcaacatggc ctgaaccgac tggtattgag catgggcggc gacggcgttt
     6421 attacagcga tatcagcggt gaaaatggct ggtctgcgcc gatcaaaacc aatgttatta
     6481 atgttaccgg agcgggcgat gccatgatgg cgggacttgc ttcgtgttgg gtagacggaa
     6541 tgccgtttgc cgaatctgtt cgtttcgcac agggatgttc gtcaatggcg ctctcctgtg
     6601 aatacaccaa taaccccgat ttatcgattg ccaacgttat atcgttagtg gagaacgcag
     6661 aatgtctgaa ttaaaaattt cccctgaatt attacaaatt tctccggaag tgcaggacgc
     6721 tttaaaaaac aaaaaaccgg ttgtggcgct ggaatcaacc attatttctc acgggatgcc
     6781 gttcccacaa aatgcccaga tcgcaattga agtagaagaa actattcgta aacagggcgc
     6841 agtacctgcc actatcgcca ttattggcgg cgtgatgaaa gtgggtttaa gcaaagaaaa
     6901 aattgaatta ctgggtcgtg aagggcataa cgtgaccaaa gttagtcgtc gcgatttacc
     6961 ttttgttgtt gccgccggaa aaaatggcgc aaccactgtg gcttcaacga tgattattgc
     7021 ggcgcttgcc ggaattaaag tatttgccac cgggggaatt ggtggtgtgc atcgcggggc
     7081 ggaacatacc ttcgatattt ctgccgattt gcaagaactg gcaaatacta atgtcaccgt
     7141 tgtttgtgcc ggggcgaaat ctattctcga tttaggatta accactgagt atttagaaac
     7201 cttcggtgtg ccgttaattg gctatcagac taaagcgctg cctgcgtttt tctgccgtac
     7261 cagctcgttt gacgtcagca ttcgtctcga cagcgccagt gaaattgccc gtgcaatggc
     7321 ggtgaaatgg caaagcgggc tgaacggtgg cctcgtggta gcgaacccga tcccggaaca
     7381 gtttgcgatg ccggaggaat ctatcaatgc agccatagat caagccgtcg ccgaagccga
     7441 agagcagggc gttattggta aagaaagtac accgttcctg ctggcgcgcg ttgctgagct
     7501 gaccggcggt gacagcctga aatccaatat ccagctggtg ttcaacaacg ccattctggc
     7561 gagtgaaatt gccaaagaat accagcgtct cgcgggttaa tttctgacgg gcagaaagcc
     7621 gtcgcccgta ctcatcaagc ataccaaaac ctggcgctga cgccgggttt actggcatga
     7681 agggaaaatc attatggata taatgagaag tgttgtgggg atggtggtgt tactggcaat
     7741 agcatttctg ttgtcagtga ataaaaagag catcagtttg cgcacggttg gagccgcact
     7801 gctgctgcaa atcgctattg gtggcatcat gctctacttc ccaccgggaa aatgggcagt
     7861 agaacaggcg gcattaggcg ttcataaagt gatgtcttac agtgatgccg gtagcgcctt
     7921 catttttggt tcgctggttg ggccgaaaat ggatgtcctg tttgacggtg cgggttttat
     7981 cttcgccttt cgcgtacttc cggcgattat tttcgttact gcgctcatca gtctgctgta
     8041 ctacattggc gtgatggggc tgctgattcg catccttggc agcattttcc agaaagccct
     8101 caacatcagc aaaatcgaat cttttgttgc ggttaccact attttcctcg ggcaaaatga
     8161 gatcccggcg atcgttaaac cgtttatcga tcgcgtgaat cgcaacgagt tgtttaccgc
     8221 aatttgtagc gggatggcgt ccattgctgg ttcgatgatg attggttatg ccggaatggg
     8281 cgtaccaatt gactacctgt tagcggcatc gctgatggcg atccccggcg gtattttgtt
     8341 tgcacgtatt cttagcccgg caaccgagcc ttcgcaggtc acatttgaaa atctgtcgtt
     8401 cagcgaaacg ccgccaaaaa gctttatcga agcggcggcg agcggtgcga tgaccgggct
     8461 gaaaatcgcc gctggtgtag cgacggtggt aatggcgttt gtcgcaatta ttgcgctgat
     8521 caacggcatt atcggcggaa ttggcggctg gtttggtttc gccaatgcct ctctggaaag
     8581 tatttttggc tatgtgctgg caccgctggc gtggatcatg ggtgtggact ggagtgatgc
     8641 caaccttgct ggtagcctga ttgggcagaa actggcgatt aacgaattca tcgcttacct
     8701 gagtttctcc ccatacctgc aaacgggcgg cacgctggaa gtgaaaaccg tggcgattat
     8761 atcctttgcg ctttgtggtt ttgctaactt tggttctatc ggtgttgtcg ttggcgcatt
     8821 ttcggctatt tcgccaaaac gtgctccaga aatcgcccag cttggtttac gggcgctggc
     8881 agcagcaacg ctttccaacc tgatgagtgc gactattgcc gggttcttta ttggtctggc
     8941 gtaatgctcg atttgtagac cggataaaac gtattaagcg ttgcatccgg caatcgcgct
     9001 attgcaaata atttttactg catcatcccg gagaatttat tctccgggtc catctccagc
     9061 gccagtcctg acaactgctt tctgttttta attagatatc ctttcttgct tttaattaat
     9121 aaaccgtcgt gaatgaattg cgcgagaaca tataaaagat gtcgataaga aacgcctaaa
     9181 tactctgccg cttgcgtatg cttttcgtga taaagatcac cttcttgtga gagtaatata
     9241 aatgctgcca ggcgattcat taacggaaat gattgattct gagttaaaga aacaatatta
     9301 cgataatttt tatgacttaa ggtgacgcaa agttttcgta aaaatagggt gtcgtttaat
     9361 aacagcggac ggtaatgttt catagggagc gcaaggcacc aacactcttc aatagcctgc
     9421 accgcacgcg gttcatggtc tttgtcgatt aactcaatct cgccaataaa acagggagca
     9481 gcaaagaaat cgatcagcga cacgcgacca ttagcaagcg tggcgtaaag cctggcgcgg
     9541 cctcgcgtca gataaaacag ccatgacggc tgttgccctt cctgcacaat gtagtcacgc
     9601 gctaaaaaat gaaacagccg cgtatcggct gaaacatccg ttgaaaagca atccttaaac
     9661 gcagattcac tcatcaattg ctgcttaagg tcattattgt ggatttcttt cacgatgttt
     9721 atccgctatg agatttctca tatttagcat acgtacgctg ttgttaagat tcaatcatca
     9781 gcccggtcga attttctttg ctgtagaaaa aaataacgca actggaaaca gaggaaataa
     9841 acaatggaaa agagaaaaat tattctggat tgtgagccgg gtcatgatga tgctattgca
     9901 atgatgatgg cagcgaaaca tccggcaata gatttattag gcatcactat tgtagcgggt
     9961 aatcagacgc ttgataaaac attaattaat ggcctgaatg tttgccagaa actggagatt
    10021 aatgttccgg tttatgcggg gatgccgcaa cccattatgc gtaaacaaat cgttgccgat
    10081 aatattcacg gtgaaaccgg actggatggc ccggtattcg agccgctgac ccgccaggca
    10141 gaaagtaccc atgcggtgaa atatatcatc gataccctga tggcaagcga tggcgatatc
    10201 actctggtgc cggttggtcc gctttcaaat atcgcggtgg caatgcgtat gcaacccgca
    10261 atcctgccca aaatccgtga aattgtgcta atgggcggcg cttacggtac aggcaacttc
    10321 accccatctg ccgagttcaa tatctttgcc gacccggaag ccgcccgcgt ggtgttcacc
    10381 tccggcgttc cattagtgat gatgggcctc gatcttacca accagaccgt ttgcaccccg
    10441 gatgtaattg ctcggatgga aagggtaggc ggccctgccg gagagctgtt cagcgacatc
    10501 atgaacttca ctctcaaaac gcagttcgaa tactacggcc tggctggcgg cccggtgcat
    10561 gacgccacct gcattggtta tctgatcaac cctgatggca ttaaaaccca ggatatgtac
    10621 gtcgaagtgg acgtcaacag cggcccttgc tacgggcgta cggtctgcga cgaactgggc
    10681 gttcttggca agcccgctaa taccaaagtc ggcatcacta ttgataccga ctggttctgg
    10741 ggcttagtcg aagagtgcgt gcgcggctac atcaaaaccc attaagtgtt gataacaagc
    10801 cgggacagta tcgcaagatg caggcccggc tatttctgat gggcagaaag ccgtcgccca
    10861 aaatgacatg aagagataat cactatggat gtcatgagaa gtgttctggg aatggtggta
    10921 ctgctggcaa ttgcgttttt actgtcagta aacaagaaga agatcagcct gcgtaccgtt
    10981 ggcgcggcgt tagtgttaca ggtcgtgatt ggcgggatta tgctttggtt accgccaggg
    11041 cgttgggtcg cagaaaaagt cgcttttggc gtccacaaag tgatggcgta cagcgacgca
    11101 ggtagcgcat ttatcttcgg ttctctggtc ggaccgaaaa tggatacctt atttgatggc
    11161 gcaggattta tctttggttt cagggtatta ccggcaatta tcttcgtcac tgcactggtg
    11221 agtattctct actacatcgg tgtgatgggg atcttaattc gcattctcgg cggtatcttc
    11281 cagaaagctt taaatatcag caagatcgag tcattcgtcg cggtcaccac cattttcctc
    11341 gtgctaaacg aaattccggc gatcgtcaaa ccctttatcg atcgtctgaa tcgcaatgaa
    11401 ttatttacag cgatttgtag tggcatggcc tcgattgctg gttcgacaat gattggttac
    11461 gccgcactgg gcgtacctgt ggaatatttg ctggcggcat cgttaatggc gatccctggc
    11521 gggatcttgt ttgcccgcct gttaagcccg gctacggaat cttcgcaggt ttcttttaat
    11581 aacctctctt tcaccgaaac accgccaaaa agcattattg aagccgatgc gacaggggca
    11641 atgaccgggc tgaaaatcgc cgccggtgta gcgacagttg ttatggcatt tgtcgccatc
    11701 attgcgttaa ttaacggtat tatcggcggc gttggcggct ggtttggttt tgaacatgcc
    11761 tcgctggagt ccattttagg ttacctgctg gccccacttg cgtgggtaat gggtgttgac
    11821 tggagtgatg caaatcttgc cgggagtttg attggacaga aactggcaat aaatgaattt
    11881 gtcgcttatc tcaatttctc accctatctg caaacggctg gcactctgga tgctaaaacc
    11941 gtggcgatta tttccttcgc gttgtgcggt ttcgctaact ttggttctat cggggtggtg
    12001 gtgggggcgt tttctgcggt tgcgccacac cgtgcgccgg aaatcgccca gcttggttta
    12061 cgcgcgctgg cggcggcgac actttctaat ctgatgagtg ccaccattgc cgggttcttt
    12121 attggtttag cgtagtgtgc aatttatgag ccggattaga tgcggtcaag cgtcgaatcc
    12181 ggcaacattg ttacaccatt ggcactaatg acagcgcgtt atcggcagac agggtagggt
    12241 tgtttaagct gccgctggcg cgtgaaattg ccgcgcaggc catcgcaaaa cgggcgctgt
    12301 cacggaaatt gtttccctcc agaaagctat ataccagacc cgccataaaa ccatcgtcag
    12361 caccaaaact gtctaccgtc gtatgcgctg gcacagtcag caaaaattgt tcaccatcct
    12421 tttcgctgca ataaactgac tcatcgggca aataaacaaa cagttgctga acaccttgct
    12481 gatgcaatgc attcactgcg gcattacggt cagcatcgct ggtgatcgcc tgtccccata
    12541 aaatttccag ctccggtaaa gtgggtttca gggtgtgaat atgcgccagc cagtgtttga
    12601 ttttgcccgc tttgaattct gaaacggtat cgacaaacac cgggatctca tcagcgaggg
    12661 taaagaccca ttccagcgcc tcggctgtca ggttgcaatc aaccagtacc acgcccgcat
    12721 gacgaagtaa atcgcgcgac ccgttcagta gttgcggtgt caactgttcc agcagatggg
    12781 tatcgttaat cgccagcacg gtttgatcgt ctcgattggc aattgccaga tacgtcgatg
    12841 tgctttgacc atgcaaacga acgcagccgg agacattcac gccggcacgg cgcgtttctt
    12901 ccaggagcat ttcgccataa aagtcatcgc caatcactga aagcaaatgg acgtcacggc
    12961 ctaacagcgc cagattgtgg gcgatattgc gtccaacgcc gcctgccgaa caatgaattg
    13021 taccgggatg agaagccgct tgcgggtaat ggatatccgc catcccgcga atatccatat
    13081 tgattgtccc caccactacg cagtattcct gctcggtgag aatgtaacct ttgcctttaa
    13141 tccggccttt gcgcattaaa tccataatat gcgccgcaac gcgcgaacga ctgatttgca
    13201 gcatgtccgc aatttcgttc tgctgaatca gcgggttacg ccgtaaaatt gcaaggatct
    13261 ccttttcccg gttattcatc ttcaggctac cgctttttca gtttgttgcg ctttcagcca
    13321 ggcgatctct tctgcccaga tatccgggtt gatggtttca aggatcagcg gaatgccgtc
    13381 gaaacggtcg ttctgcataa tccagcggaa cgcatcatga ccgatattgc cttcaccgag
    13441 gctatgatgg cggtcaacgc ggctgccaaa ggtacttttc gcatcgttaa ggtgcatccc
    13501 gcgcagatac ttaaagccga caatacgggc aaaatcagcg aacgtttttt cgcattcggc
    13561 tggagtacgc aaatcatacc cggcagcgaa agcatggcag gtatcaatgc agacgccgac
    13621 gcgggattta tcttccacgc cgtcgataat cgccgcgaga tgttcgaatt taaaccctaa
    13681 gttactgccc tgaccggcgg tgttttcaat caccgctgtt acaccttgag ttttatccag
    13741 cgcaatgttg atggattcgg caatacgcgc aaggcaatcc tcttctgaaa tctgcatcag
    13801 atggctgcca gggtggaagt tgagcaaaga aagccccagt tgttcgcaac gctgcatttc
    13861 atctataaag gcatcgcgcg atttttccag agcttcagtg accggatgtc cgaggttgat
    13921 cagataactg tcgtggggaa gaatttgcgc cgatgtgtag tgatattttt cacaggcggc
    13981 tttgaattca tcgatggttt gcgtcgtgag cggtgcggca cgccactgac gttggttttt
    14041 ggtgaacaag gcaaacgcgg ttgcgtcgat ttcggcggcg cgaattgcgg catttgccag
    14101 accgccagca gcactaacgt gcgctccaat gtatttcatg cgaggactcc tgttaaaccc
    14161 gctggaggaa aacggtaatg atagcgggtt aacaggagca agaagtagtg gtttatgcga
    14221 tgacgctttg aatcacatag ttaatcgcac caccaccaac aatcagccag gcaaacagta
    14281 ccagtgccat cagcagaggt ttcgccccag cttttttcag cgcgctgacg tgagtggtca
    14341 gacccagcgc cgccatcgcc attgccagca ggaaggtatc cagcgttacc agcatgttca
    14401 ccacgctctg cggtaacagg tggaacgagt taaagatggc aactacgatg aacaagatgg
    14461 caaaccacgg aatagtgatt ttgcttttct cgccgctgtt cgccccagac agctgtttaa
    14521 cacgcgccgc cagcaggatg aggaacggag ccagcatcat cacgcgcagc attttggaaa
    14581 taactgctgc gttttctgca tccgggctga tggcatgacc tgccgccacc acctgcgcca
    14641 cttcgtgcac agtagaacca atgtagatac cgaaagtttc cggactaaac cattgagaca
    14701 tcagcggata tatcgccggg tagaggaaaa tcgcgacggt cccgaagata acaacggttg
    14761 caacagccac ggttacttta ctggcttccg ctttcactac cggctcagtc gccagtaccg
    14821 cggcagcacc acagatactg ctaccggcac cgatcaacca actggtgtgc ttatccagac
    14881 caaacacttt ctgccccagg aagcaagcca gcaggaaggt actggacagc gtcagcacgt
    14941 caatgatgat cccactgata ccgacatcgg caatttgcga gaacgtcaga cggaagccat
    15001 aaagaatgat acccagacgt aataaatatt gcttggcaaa cagcacacca ccgtcacagc
    15061 ttttccagat gtgcggatag atggtgttgc ctaaaaccat ccccaacaag attgcgaggg
    15121 tgagggcact aaacccggca cccgcaaccg cgggaatgga accaccccac agggcgaccc
    15181 cggtgataac tgcactcagg gctaaccccg gaataaaatg ccacagtgta cgatgttgtt
    15241 tctgtaaggt gatattcgtc ataaccctct cctttaaccg ggcataaggt tacgactaac
    15301 tggtttaaaa ataaaattga ttatatattt ataattaatc tttataagtg gtaagcgact
    15361 atgcacatca ccctccggca gttggaagtt tttgcagaag tattgaaaag tggatcaacc
    15421 acccaggcgt cggtgatgct ggcgttgtcg caatcagcag tgagcgcagc cttgactgac
    15481 ctggaagggc agcttggcgt tcaactgttt gatcgcgtgg ggaaaagact ggttgttaat
    15541 gaacacgggc ggctgctcta tccgcgagcg ttggcactgc ttgaacaggc ggttgaaatc
    15601 gaacaactgt ttcgcgaaga caacggcgcg attcgtatct atgccagtag taccatcggt
    15661 aactacattc tgcctgcagt tatcgcccgt tatcgccatg attatccgca gttgccgatt
    15721 gaacttagcg ttgggaacag ccaggacgtg atgcaagcgg tgctggattt ccgcgttgat
    15781 attggcttta ttgaaggacc gtgccacagc actgaaatca tttctgaacc gtggctggaa
    15841 gacgagctgg tggttttcgc cgcgccgact tcgccgttgg ctcgtggtcc ggtcacctta
    15901 gaacagctgg ccgctgcgcc gtggatcctg cgtgaacgcg gttccggcac gcgggagatt
    15961 gtcgattatc tgttgctgtc acatttaccg aagtttgaga tggcgatgga attaggtaac
    16021 tccgaggcaa tcaaacatgc ggtgcgtcat gggttgggaa ttagttgcct gtcgcgacgt
    16081 gtgattgaag atcaattgca ggcaggcaca ttaagtgaag ttgcggtccc tctgccgcgc
    16141 ctgatgcgta cgttgtggcg tatacatcat cggcaaaaac acctttccaa cgcgctacgg
    16201 cgctttctgg actattgcga tcccgcaaat gtgccgcgtt aagttgctgt acaagaacat
    16261 gctggtgctg tgtcgatttc gtgacgcagc gccttcagca tgcattcgcc agaaaagaga
    16321 ttggctgctt tacttataat ccctgggcga tcatgaaggt gtcttataac cgtgtatttc
    16381 tgccggaagg attgccaatc gtctgctaca atcgcgccat catttttaag atggatagca
    16441 tttttgtatg ggttccgaaa ctaaaactac agaagcgccg ggcttacgcc gtgaattaaa
    16501 ggcgcgtcac ctgacgatga ttgccattgg cggttccatc ggtacaggtc tttttgttgc
    16561 ctctggcgca acgatttctc aggcaggtcc gggcggggca ttgctctcgt atatgctgat
    16621 tggcctgatg gtttacttcc tgatgaccag tctcggtgaa ttggctgcat atatgccggt
    16681 ttccggttcg tttgccactt acggtcagaa ctatgttgaa gaaggctttg gcttcgcgct
    16741 gggctggaac tactggtaca actgggcggt gactattgcc gttgacctgg ttgcagcaca
    16801 gctggtcatg agctggtggt tcccggatac accgggctgg atctggagtg cgttgttcct
    16861 cggcgttatc ttcctgttga actacatctc tgttcgcggc tttggtgaag cggaatactg
    16921 gttctcactg atcaaagtca cgacagttat tgtctttatc atcgttggcg tgctgatgat
    16981 tatcggtatc ttcaaaggcg cgcagcctgc gggctggagc aactggacaa tcggcgaagc
    17041 gccgtttgct ggtggttttg cggcgatgat cggcgtagct atgattgtcg gcttctcttt
    17101 ccagggaacc gagctgatcg gtattgctgc aggcgagtcc gaagatccgg cgaaaaacat
    17161 tccacgcgcg gtacgtcagg tattctggcg aatcctgttg ttctatgtgt tagcgatcct
    17221 gattatcagc ctgattattc cgtataccga tccgagcctg ctgcgtaacg atgttaaaga
    17281 catcagcgtc agtccgttca ccctggtgtt ccagcacgcg ggtctgctct ctgcggcggc
    17341 ggtgatgaac gcagttattc tgacggcggt gctgtcagca ggtaactccg gtatgtatgc
    17401 gtctactcgt atgctgtaca ccctggcgtg tgacggtaaa gcgccgcgca ttttcgctaa
    17461 attgtcgcgt ggtggcgtgc cgcgtaatgc gctgtatgcg acgacggtga ttgccggttt
    17521 gtgcttcctg acctccatgt ttggcaacca gacggtatac ctgtggctgc tgaacacctc
    17581 cgggatgacg ggttttatcg cctggctggg gattgccatt agccactatc gcttccgtcg
    17641 cggttacgta ttgcagggac acgacattaa cgatctgccg taccgttcag gtttcttccc
    17701 actggggccg atcttcgcat tcattctgtg tctgattatc actttgggcc agaactacga
    17761 agcgttcctg aaagatacca ttgactgggg cggcgtagcg gcaacgtata ttggtatccc
    17821 gctgttcctg attatttggt tcggctacaa gctgattaaa ggaactcact tcgtacgcta
    17881 cagcgaaatg aagttcccgc agaacgataa gaaataagtt ttctcccttc cttgctaagc
    17941 cctctcaacc gagagggctt tttcaattcc atttccctga caaatcatgc ggatataaaa
    18001 tttaacattt ggattgataa ttgttatcgt ttgcattatc gttacgacgc aatcaaaaaa
    18061 ggctgacaaa tcagaggctg ttccggcttt ctgggatgat cacctgcata caaataagtc
    18121 caccgcgatg ctgccgtacg caaggggacg taaagaagat gtgagcgata cccattttat
    18181 tttcgtagtt acctcatgga gatatggaat gtttaggttg aaccctttcg tacgggtcgg
    18241 gctgtgtttg tccgctattt cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga
    18301 aacgatggtt gtcactgcat cttccgtgga acaaaatctt aaagatgcac ccgccagtat
    18361 cagcgtcatt acccaggaag acctgcagcg aaaaccggta cagaatctga aggatgtcct
    18421 caaagaagtg cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat
    18481 tcgtggtctg gacagcagct acaccctgat tcttgtcgac ggtaaacgcg ttaactcccg
    18541 caatgccgtc ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga
    18601 acgtattgaa gtggttcgtg gcccgatgtc gtcgctatac ggctccgatg cgctcggcgg
    18661 tgtagtgaat atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga
    18721 taccaccatt caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag
    18781 tggaccatta attgatggtg tgctgggaat gaaagcttac ggcagcctgg caaaacgtga
    18841 aaaggatgac ccgcaaaact caacgaccac cgataccgga gaaacgccgc gtattgaagg
    18901 attctccagc cgcgacggca atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt
    18961 tactgccgga tacggtttcg accgtcagga tcgtgattcc gactagctgg acaaaaaccg
    19021 cctggaacgc cagaactact gcgtcagcca taatgggcgt tgggattacg gcaccagcga
    19081 actgaaatcc tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac
    19141 ttccgaaagc aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt
    19201 tctcacggtt ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg
    19261 gggaaccagc tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg
    19321 gatcttcgag ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg
    19381 tgaacactgg agtccgcgtg cctacctggt ttataacgcc accgacaccg taacggtgaa
    19441 agggggctgg gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac
    19501 gagcaattcc tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac
    19561 cagcgaaagt tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggg
    19621 tgaatccagc gttatcgttt tccgtaacga tgtgaaagat cgtatcagca tcagccgtac
    19681 gtctgacgtc aacgctgcac cgggctacca aaactttgtt ggttttgaga cgggcgctaa
    19741 cggacggcgc ataccggtat ttagctacta cagcgttaac aaagctcgta ttcagggcgt
    19801 ggaaaccgaa ctgaaaattc cgttcaacga tgaatggaaa ctgtcgatca actacaccta
    19861 caacgatggt cgtgatgtca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca
    19921 tactgctaac ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt
    19981 ttctggtcac tataccgggc agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg
    20041 ttataccatc tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc
    20101 aggcgtgctg aaccttggcg acaaggatct cagtcgtgac gactacagct ataacgaaga
    20161 cggacgtcgt tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac
    20221 cgggcggact ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa
    20281 gaggcgatga agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac
    20341 gctttctggc gcgcggcttc tgccaatacc gcaggttgca actggtcggc aagaaactga
    20401 tcattatcgc cctgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc
    20461 agcgcgcaac tatcccattc cagccatgca tttttgtcct cacctaaata ggtgctaaac
    20521 gctttgattc cccacgggac gctgcacgga ttcacaatgg gcgcaaaggc cgaaacgctg
    20581 gtgtatttac ccggattttt cagcgccata atcagcgcac cgtgaccacc cattgagtga
    20641 ccgctaatgg cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca
    20701 tcgcgcagat aatcatacat ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga
    20761 taaaagcctg cgccctggcc taaatcgtag ccatcgtcgt tggcaacctt ttcgccgcgc
    20821 gggctggtgt ctggcatcac cagtacaatc cccagttccg ccgctacccg ctgggcaccc
    20881 gccttggtgg tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt
    20941 ggcggggtgt gatcacgagg gggtgggaga aagatactga acgtcatcgg gcagtttaag
    21001 gtactggagt cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc
    21061 atttccatgc gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca
    21121 atgtgctgtg agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat
    21181 gaaaggcagc ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc
    21241 acgcacaata atcaagctgt aaatcagctt aataactttg cccccacgca gggcggaggc
    21301 gtcacacctg caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca
    21361 tgaagcgtta gttgcgagag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga
    21421 taacgaaacg cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa
    21481 tctcgacctg gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt
    21541 cgatgaaatt ttctccggtc tggattacgc caatttcccg aaaatcaccc tcattgaaaa
    21601 caaaatgaag gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga
    21661 acaccatttt gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt
    21721 gatcggtctg tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca
    21781 ggaacgtctg acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt
    21841 ggctgtctcg atcgacgcgg tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac
    21901 cagtgccacg acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca
    21961 cgagtttctg cgcgctgtgc gccatcacaa ctgattaaaa ggcaggaacc atggagcgca
    22021 acgtcacgct cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca
    22081 gcgcctttgg gctaccaaag gcggcttatc tcaatcccgc ctggtacgat gctattacgc
    22141 cgcaggatgc atggacctgg gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc
    22201 tttttgcgct gctgtttggt gctggcctgc aaatgttgct gccccgtggc agacgctgga
    22261 tccagtcgcg gttaacgctg ttagtcttgt tgggctttat tcacggttta ttgttctggg
    22321 acggcgatat tctgctggct tacgggctgg tgggcttaat ctgctggtgg ctggtgcgcg
    22381 atgcgccatc ggtaaaaagc ctgtttaata ccggcgtcat gctttatctg gtggggcttg
    22441 gcgttttgct gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg
    22501 atgcatcggc tattttgtat gaaaaatact ggaagcttca cggcggcgtt gaagcgatca
    22561 gtaatcgtgc cgatggtgtt ggcaatagtt tactggcact gggcgcacag tatggctggc
    22621 aactggcagg gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc
    22681 agttcagctt acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca
    22741 ttaaccttcc tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct
    22801 tcttacttca aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc
    22861 tgttttatgg cttctggccg caattgagcc gctttaagct ggtgcttgcg atcgcctgcg
    22921 tcggacggat ggcgctgacc aactatctat tgcaaacgct gatttgtacc acgctttttt
    22981 accacctcgg tttgtttatg cattttgacc gcctggagct gctggcgttt gttattccgg
    23041 tatggctggc gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg
    23101 tggaatggct ctggcgtcag ttaactttgc gtgctgccgg actggcaata tctaaaacat
    23161 caagataacg atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt
    23221 gactcgattc acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat
    23281 ttactgcaat ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg
    23341 ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg
    23401 acacgcgtga agcagtaatg aaagccgtga gtgagctgga ttatcggcca aacgccaatg
    23461 cccaggcgct ggcaactcag gttagcgaca ccattggcgt ggtggtgatg gacgtttctg
    23521 atgcgttttt cggcgcgctg gtaaaagcgg tggatctggt cgcccagcag catcagaaat
    23581 acgtgctaat cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt
    23641 taattcgcca gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac
    23701 tggcacaatt tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt
    23761 acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt
    23821 tgctgaataa cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg
    23881 acgccatgcg taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg
    23941 aaagctggat tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac
    24001 tgctggggcg caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg
    24061 gcgcactgac agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg
    24121 gtttcgatga tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc
    24181 ccattgcttc aatggctaaa ttagccaccg aactggcctt gcagggggca gcaggcaata
    24241 ttgatcctcg tgccgccact gttttatgcc gacgttagtg cgtcgccatt ctgtcgcaac
    24301 gcgccagaat gcggcggcga tcactaactc aacaaatcag gcgatgtaac cgctttcaat
    24361 ctgtgagtga tttcacagta tcttaacaat gtgatagcta tgattgcacc gttttaatgt
    24421 tgtaacccgt atgtaacagt gaataatcac tttcaccgag gtaatagcgt cataacaaca
    24481 attaaagccg ttttctggag cgttaccggg catggaagaa cgaattttaa aaagtgagct
    24541 tcggcgttca gtaacacttc attaactcta ctgccccgcc gagcatttat ctcaagcact
    24601 accctgcata agaaaaaccg gagataccat gaataagaag gtgttaaccc tgtctgctgt
    24661 gatggccagc atgttattcg gtgccgctgc acacgctgct gatactcgca ttggtgtaac
    24721 aatctataag tacgacgata actttatgtc tgtagtgcgc aaggctattg agcaagatgc
    24781 gaaagccgcg ccagatgttc agctgctgat gaatgattct cagaatgacc agtccaagca
    24841 gaacgatcag atcgacgtat tgctggcgaa aggggtgaag gcactggcaa tcaacctggt
    24901 tgacccggca gctgcgggta cggtgattga gaaagcgcgt gggcaaaacg tgccggtggt
    24961 tttcttcaac aaagaaccgt ctcgtaaggc gctggatagc tacgacaaag cctactacgt
    25021 tggcactgac tccaaagagt ccggcattat tcagggcgat ttgattgcta aacactgggc
    25081 ggcgaatcag ggttgggatc tgaacaaaga cggtcagatt cagttcgtac tgctgaaagg
    25141 tgaaccgggc catccggatg cagaagcacg taccacttac gtgattaaag aattgaacga
    25201 taaaggcatc aaaactgaac agttacagtt agataccgca atgtgggaca ccgctcaggc
    25261 gaaagataag atggacgcct ggctgtctgg cccgaacgcc aacaaaatcg aagtggttat
    25321 cgccaacaac gatgcgatgg caatgggcgc ggtagaagca ctgaaagcac acaacaagtc
    25381 cagcattccg gtgtttggcg tcgatgcgct gccagaagcg ctggcgctgg tgaaatccgg
    25441 tgcactggcg ggcacagtac tgaacgatgc taacaaccag gcgaaagcga cctttgatct
    25501 ggcgaaaaac ctggccgatg gcaaaggtgc ggctgatggc accaactgga aaatcgacaa
    25561 caaagtggtc cgcgtacctt atgttggcgt agataaagac aacttggctg aattcagcaa
    25621 gaaataagac tgattgggcg cagtcgttta ctgcgccctt attgtatggc caacaaggta
    25681 aaattatatg gtcagctcaa cgactccgtc ttccggggaa tacttgttgg aaatgagcgg
    25741 tatcaacaag tcctttcctg gtgttaaggc acttgataac gttaatttaa aagtccggcc
    25801 acattctatc catgcattaa tgggggaaaa cggtgcagga aaatcgacat tattaaaatg
    25861 cctgtttggt atttatcaaa aagactccgg caccatttta ttccagggta aagagatcga
    25921 tttccattcc gccaaagagg cactggaaaa tggtatttcg atggtacacc aggagttaaa
    25981 cctggtatta caacgttcgg tgatggataa catgtggctg gggcgatatc ccaccaaagg
    26041 catgtttgtc gatcaggaca aaatgtaccg cgaaaccaaa gcgatttttg atgaactgga
    26101 tattgatatc gatccgcgtg cgcgcgtcgg cacattatcc gtttcgcaaa tgcagatgat
    26161 cgaaatcgcc aaagcgtttt cctataacgc gaaaattgtg attatggatg aaccgacttc
    26221 ttcgttaacc gaaaaagagg tcaatcatct gttcactatt attcgtaaat taaaagagcg
    26281 cggctgcggt attgtttata tctcgcataa aatggaagaa atcttccagt tatgtgatga
    26341 agttaccgta ttgcgcgacg gtcagtggat cgccaccgaa ccgctggcag gactgacgat
    26401 ggacaagatc atcggcatga tggttgggcg ttctcttaat cagcgttttc ctgacaaaga
    26461 aaacaagccg ggcgaagtca tcctcgaggt gcgtaaccta acgtcactgc gccagccgtc
    26521 gattcgcgat gtctcgtttg atctgcataa aggggagatc ctcggtattg ccgggctggt
    26581 gggggcgaaa cgtaccgata ttgttgagac gttatttggt attcgcgaga aatcggctgg
    26641 caccattacg ttgcacggca aacagatcaa taaccataat gccaacgaag ccataaacca
    26701 cggatttgca ctggtaactg aggagcgccg ctcaacggga atttatgcct atctggatat
    26761 tggttttaac tcgttaattt ccaatattcg caactacaaa aataaagttg gtttactgga
    26821 taactcgcgg atgaaaagcg atacccagtg ggtgattgat tcgatgcggg taaaaacgcc
    26881 gggtcatcgg acgcaaattg gttcgctctc cggtggtaat cagcaaaagg tgattattgg
    26941 tcgctggcta ctaacgcaac cagaaatatt aatgctcgat gaaccgacgc gcggtattga
    27001 tgtcggggcg aagtttgaaa tttatcagtt aattgccgaa ctggcgaaga aaggcaaggg
    27061 gattattatt atctcctctg aaatgcctga gttgttaggg ataacagacc gtattctggt
    27121 catgagcaat ggtctcgttt ccggaattgt cgatacaaaa acaacaacgc aaaacgaaat
    27181 tctgcgtctt gcgtctttgc acctttaaga ttaggggctt cccatgagtg cgttaaataa
    27241 gaaaagtttt cttacttacc tgaaagaggg cggtatttac gtcgttcttt tagttttgct
    27301 ggcgattatt attttccagg acccaacatt tttaagtctg ttgaacttaa gtaatattct
    27361 cacccagtca tcggtgcgta ttattatcgc gctcggtgtg gcagggttaa ttgtcaccca
    27421 ggggaccgat ctttctgctg gtcgtcaggt agggctggcg gcagtggtgg ctgcgacatt
    27481 attgcagtcc atggataacg ccaacaaagt gttcccggaa atggcgacga tgccgattgc
    27541 gctggttatt ctgattgtct gtgccattgg tgcggtgatc ggtttgatca acggtctgat
    27601 tatcgcttat ctcaacgtga cgccgttcat taccacgctc ggcacgatga tcatcgtcta
    27661 tggcatcaac tcgctctatt acgactttgt cggggcgtcg ccaatttctg gttttgacag
    27721 tggcttctct acctttgctc agggctttgt cgcgctgggg agtttccgtc tctcttacat
    27781 caccttctac gcgttgattg cggtggcgtt cgtctgggtg ttgtggaaca aaacccgctt
    27841 cggtaagaac atttttgcca ttggcggtaa cccggaagcg gcaaaagtat ctggtgtcaa
    27901 cgtcggcctg aacctgctga tgatctacgc gttgtctggc gtgttctatg cctttggcgg
    27961 gatgttagaa gccggacgta tcggctctgc caccaacaac ctcggcttta tgtatgagct
    28021 ggatgctatc gcggcgtgcg tggtaggcgg tgtatcgttc agcggcggtg tggggacggt
    28081 gattggcgtg gtgaccgggg taattatttt taccgtcatc aactatggcc tgacgtatat
    28141 cggcgtaaac ccatactggc agtacattat caaaggggcg attattatct tcgccgtagc
    28201 gctggattca ctgaaatacg cgcgtaagaa gtgatgtgat tgcggatatt gccggatgcg
    28261 ctgcatccgg caatcggtgc caaatgccgg atgcgtctct ggttttacaa cgttaccggg
    28321 tgttctttct cgcctttctt aaacttcact tccccgagct caatacaacc caccgggcag
    28381 acgtgaccac aaagcagaca acccacacat ttctcggtat tacaatgcgg tgtgcgggtt
    28441 ttctcgctcc attccatcgc ctggtgaccg ccgtcgtaac aggaaatata acagcgtcca
    28501 cagccaacac atttgtcaag attgatacgg ggatagacaa tataactgcg gtctaaatct
    28561 tccgccggga caatattgtt attcgccaga cctaccatct cctgcaacga atcaaatccc
    28621 tgatcggcga gataatgcga taacccgctc gccatatctt ctacgatgcg atagccgtac
    28681 tgcataatgc cggtggtgac ctgtaacgtt gctgcgccga gcaataaaaa ctccgccgca
    28741 tcttcccagg tttcaatgcc gccgataccg ctgattggga aatcgcgcag ttctggatgg
    28801 gtgcgcattt gctgaatgaa gcgcagggcg atcggtttta ccgctttacc ggaatatccg
    28861 gaaatactcg atttaccgtt aacgatcggc ataccgattt tctgattaag atcgatattg
    28921 gtgatggatt taacggtgtt aattgccgca atgccatctg cgccgccgcg cttcgccgcc
    28981 agcgccactt cgcacatatc accgatattc ggcgtcatct tcgccagcat tggcagcgtg
    29041 gaaccccgtt tcactgcccg acaatatttt tttaccagct ccgggctttg cccgacatcg
    29101 ctacccatcg catgagaagt catttgcgga caggagaagt tacactcgat catatccgcg
    29161 ccagcttctt gcaccaggcg cgccagctcc tcccattgct gctcattttc ccccatgatc
    29221 gaagcgatca atactttgtc cgggtaatct tccttcagcc gacgcagggc ggtcagattc
    29281 tcttccaacg gatgttcagc aatctgctcc atatttttga agccgataaa accggtatct
    29341 tctttcacca gatgatcaaa acgcggcgag acttcgttgg cgataaaaaa gccgatcgtt
    29401 ttaaacacca caccgcccca acctgtgtcg taggctttgg cacacatctc atagcagttg
    29461 cctaccggcg aagaagagag gcagaacggg ttgggaaagt tcacgccgca aaaagtaatc
    29521 gaaagatctt tcgttaacat gagcaagctc cctctaatat agtgatgaat cgcctcggcg
    29581 gcttctttcc cggttttcac ggcatagacc acggttttgt caccctcaac aatatcgcca
    29641 gcagcaaaga cttgcggatc gcgggtctga taattttgtg ttttgatggt gttacgctgc
    29701 tgctctaact ccgcaaaggc atccagtctg gcatgctgac cgacggcgag aataatttta
    29761 tctgccgcca tcgtcagttc gcctgataac cgtacatgct taaacgtgac tttattgcct
    29821 tccacggcta ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggca
    29881 aactcttttt cgcttgccgg aaactcatct aactcttcac gtgctacgca agttaccgcc
    29941 tgacagccga gaactttcag cgtgctggct acgtccatcg cgacatcacc gccgccgata
    30001 attaatgcgc tttgtggaat gctgatatcg ccttgcggct gtcgtacacg ttgcaggaag
    30061 tcgacggcaa tctcaacggt cgctatgctc aaacagcggt agcccggaac cgcttgataa
    30121 ccccacggtg accagtaccg cgcggttttc cgctttcagt tgctccaggg tgagtgtttt
    30181 accgatttcg ttattgcact taatggtcac gcccattttt tcaatacggg cgatctctgc
    30241 atccagcact gattgcggta accggaattg cggaataccg ttacgcaacc agccaccggg
    30301 gtgcgcttct ttctcataaa tcgtgacgtc ataaccctgg tttgtcagtg tcacactggc
    30361 ctgcaatccg gcaggacctg cgccaataat cgcgactttg ccgagcgttt tagtaccggg
    30421 ctgataaatt tccatcccgg tttgttgttc aaaatcagta acaaaacgct gtaagcggcc
    30481 gatatcaatg ggagcatcaa caccggcacg ggtacaaccg ctttggcata atttctccgt
    30541 cgggcacact ctggcacaaa cggcaccgag ggcattattt tcgcgaattg tctcggcagc
    30601 gcctttaaaa ttacgaaagt agattgagcg aataaatttc cccggatcgg tctgtgccgg
    30661 gcaagcctga ctacagggag cgtcgtgaca taataaacag cgagaggctt ctttaatcgc
    30721 cagtaacggc gtaaaagccg gagtgagttc atccagataa ttttgttgcg gcatgtgatc
    30781 ttccttaatg ctatgagtaa ttatggcgaa cgctaaataa atattgcgtg ctgatattaa
    30841 ggctatgcgc gtggggaaat ttaaactgtg aaaggattca caaatagctg cggttcagaa
    30901 tgttatatcc gcagcttatt gagtgaagta ataattattc tcaataatta acagaaaaga
    30961 gaaaatatta acgatagaaa ataagataaa caatcatgcc gatagataaa attccgaagc
    31021 caatatatgc caccatataa gcgaagaaga gtccgggatt ttttgctcgc cagacataca
    31081 ccggttccgg tgcattttta acccgtttat caatcgcgcc tgcgcgccag cctttccatg
    31141 cttcgcggaa acaaaatatc gggataagga aaaaaacggc aacgacaaaa aactgctgta
    31201 catccataac tactttcctt gtttcttttg taattcaagt aactgttcgg gtgtaacggc
    31261 gggatacccc tgcgcatctt ccggcacctg gtgcatagcc ggaataggga ccagcggccc
    31321 taaaaaacgc ggttcacgtt taaaaatata aaggtcagcc agcgcaccga aacgggcggc
    31381 aaattcgcga atacgtactg acagcatatc tttcggtgac ggtacggcat aacactgagc
    31441 ctgaatcccc atatgcagcg caataaataa tgctcgctca cagtggaaac gttgggtgat
    31501 aataatgaaa tcattagtat cgaaaacttt gcgtgtacgc acgatggaat ccagcgtacg
    31561 aaagcctgcg taatcgagaa caatatctga tgggtcgaca ccagcagcga ttaaatcttt
    31621 gcgcatggtc atcggctcgt tataactttg caatgcgtta tcgccgctca gtaatagata
    31681 atttacctta ccgctgttat aggcattaat cgctccttga atgcggtagc gataatactg
    31741 attaataacg ccggtacgat aatattttgc tgtgccgagc accacgccga cctggcggta
    31801 ggggagatcc tgcaattcgt cgtagatata aggcgcggtt ttccagctca tccagcgatc
    31861 gaggccgagc acagtcaaca gcagcaagcc gatcaggact aacaggctga ggaacacgcg
    31921 ctttaacatg caggagactc gagctaagga aaatttccat caggctactt tacccgctgg
    31981 ttttgcgcaa gaaacggcaa actgtttgcc gggaatttcg acaccaggag agggctggca
    32041 agccggacaa ggcgttcacg ccgcatccgg caccaggctt aagcgagaag cacgcggtcg
    32101 atactgtgac agccgagagc tttcagcgtt gcggaggtgg catcccactg aatcaacggc
    32161 gcatcggctt tttctgccag aaccgcgcgc tggatatccg ggtaatcata acccttgaga
    32221 ttcaacagaa ttaacgcccc ctgcaacggc ggcagagtcg ggttgaatgc ggcgttttca
    32281 gcgtagctgc cgctgaaaat acgaccgtct ttacattcca gcgcgacacc gcttggtgac
    32341 ttactgtaag gcatgtgcga acggtttgcc gctgcaatcg ccgcctgaga aagcgcatca
    32401 ccagtcagcg catagccgtg atcctgctcg tccatcagca gcgttttaat ctccagatct
    32461 ttcggcccaa aggcatccgg cagatagtca cgcagcgcgt gtgcctcgcg gcccggcaaa
    32521 tgaatacgca gatccagacc gctgttcagt tcattcataa actgacggca gtgaccacaa
    32581 ggcgtatagt taacggtgat ggctgcaagc gctttttcac cactcaacca ggcgtggctg
    32641 atcgcgcttt gttcggcatg aacggtttgc tgcattgttg caccaataaa ttccatattg
    32701 gcaccaaaat accaggttcc gctcacaccg cgcgcaattg cgccaacatt aaaattcgac
    32761 aatggcgtac gcgcacaggc cgccgccagc ggaagtagtg cgaatgccag cgcgtcttcg
    32821 tccagccccg ttgcgctctt cagcgatgag acttgctccc cggtcaacaa agcggggaag
    32881 tacttgtctg ccagaatagg ttccagtgca gattgcaagt tatccgcaag ttgggcaaaa
    32941 gcggtttgaa aacgtggatg catggcgttg cctcataatc atgtaatggg ataacagttt
    33001 acgaacccgt tatggcttta tatgtgatct gaatctcatt aattatgcaa cacgtgaaac
    33061 ttacatcaaa aatgcgcgac gtatcgcaaa ttttagccca ttactgccag aataatcggg
    33121 aaaaggaacg gtgcgatcag cgaagtaatt atcccgcata acaccagcgc tagcgaacta
    33181 aatgcacctt cctgataatc cagctcggcg cagcgcgccg taccgagggc gtgcgaggca
    33241 gtccccattg ccagaccgcg cgcagctttg gtacgaatac gcatcgcatt aagcaatgta
    33301 tggccaaata ccgcgccgag gatgccgacg aaaatcacgc aaactgcgct gattgccgga
    33361 ataccgccaa tactgccgcc aaccgccatt gcaataggcg tggtgactga tttcggcagg
    33421 attgacgcgg cgatttccgg tgaagcgccc atcaacaatg ccacggaagt ccccgtcacc
    33481 atcgcaacca cgctgccgat aaaacagatg gtgatgatcg atttccagcg cgcgcggatc
    33541 tggtgtagct gctcatataa aggataggcc agcgcgacga ccgccggttg cagcagatcg
    33601 tttagcactt cgctaccttt aaagtaactg tcgtaagaga tgccggtcag cattaaaaaa
    33661 ggaatgatca ccaccattgc taccagcaac gggttaagca ggggaaactt ataccgtgcc
    33721 gccagtttgc gggcggcaaa aaagacaatc aacgtcagcg gtaatgacca ccagatattc
    33781 gccatcattc ttctgatcct ttctgaccta cgactttacg ttcaccgtgt accagttgtg
    33841 aactccagct caccaccaga aaaaccacca gcgtactgac agcacaggaa accactaccg
    33901 ggccaaactg cgcgcggagc aaatcaaaat attgcatgac gcctacgcca atcggcacaa
    33961 acaatagcgc catatagcga atcagtacgt agcaccccgg attgacccat ttcgccggaa
    34021 gaatttgcaa ggccagcagg acaaacagga tcagcatccc gatgatgctg cccggaatgg
    34081 ttaccggtag cagggaagca atgaaaatgc ctgcatacag gcaggcataa atcaggacga
    34141 aagcgcgtaa atattgccag ataatgttca gtgtcttact cataatcaac accccagatg
    34201 agatggattc atcatacaat taattcgtaa aacgtgctac cgatcacatt aagaaatcag
    34261 cgcttatttt ttcgctaccc ggtattacgc aatgagcagc gaactcaggc atacatcaag
    34321 catagagagg gattgcagtt tgcagcaatc cctctttgca gacctttaat ccgctgacca
    34381 taggcgctaa catagcgcct catttttttg cgggtgatga tatacgtgtg ttactggcac
    34441 cgatggaggg agtgcttgac tctctggtgc gtgaattgct gaccgaagtt aacgactacg
    34501 atctgtgcat caccgagttt gtccgcgtgg tggatcaact gctgccggta aaagtctttc
    34561 atcgcatttg ccctgagcta caaaacgcca gccggacacc atctggtacg ctggtgcgcg
    34621 tgcagttgtt aggtcagttc ccacaatggc tggcagagaa cgccgcccgt gcggtggagt
    34681 taggttcctg gggcgtggat ctcaattgcg gctgcccgtc gaaaacggtt aacggtagcg
    34741 gcggcggggc gacgttactc aaagatcctg aactcatcta ccagggggca aaagcgatgc
    34801 gtgaagctgt accggcgcat ttgcctgtca gcgtgaaagt gcgtctgggc tgggacagcg
    34861 gtgagaagaa atttgaaatc gccgatgcgg ttcaacaggc tggcgctacg gagctggtgg
    34921 tgcatgggcg gacgaaagag cagggttacc gcgcggagca tattgactgg caggcgattg
    34981 gcgagattcg ccagcggctg aatattccgg tgattgccaa cggtgaaatc tgggactggc
    35041 agagcgcgca acaatgcatg gcgatcagcg gctgcgacgc agtgatgatt ggtcgcgggg
    35101 cgctcaatat tcccaacctg agccgggtgg taaaatataa cgaaccgcga atgccgtggc
    35161 cggaggtggt tgctttgctg caaaaatata cccgtctgga aaagcagggc gataccgggt
    35221 tatatcacgt tgcgcggatt aaacagtggt tgagttattt gcgtaaagaa tacgatgaag
    35281 caacggaatt atttcagcat gttcg
//
LOCUS       DADLTF010000024        34775 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.024, whole genome shotgun sequence.
ACCESSION   DADLTF010000024 DADLTF010000000
VERSION     DADLTF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.024"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(19..94)
                     /locus_tag="JN749_001133"
     tRNA            complement(19..94)
                     /locus_tag="JN749_001133"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(59..61),aa:His,seq:gtg)
     gene            complement(153..229)
                     /locus_tag="JN749_001134"
     tRNA            complement(153..229)
                     /locus_tag="JN749_001134"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Arg,seq:ccg)
     gene            complement(332..1717)
                     /locus_tag="JN749_001135"
     CDS             complement(332..1717)
                     /locus_tag="JN749_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7367617.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN749_001136"
     CDS             complement(1908..2648)
                     /gene="rffM"
                     /locus_tag="JN749_001136"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HAY7367618.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN749_001137"
     CDS             complement(2651..4003)
                     /gene="wzyE"
                     /locus_tag="JN749_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HAY7367619.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN749_001138"
                     /gene_synonym="wecF"
     CDS             complement(4000..5079)
                     /gene="rffT"
                     /locus_tag="JN749_001138"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HAY7367620.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN749_001139"
     CDS             complement(5076..6326)
                     /gene="wzxE"
                     /locus_tag="JN749_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HAY7367621.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRFGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(6328..7458)
                     /locus_tag="JN749_001140"
     CDS             complement(6328..7458)
                     /locus_tag="JN749_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-glucose transaminase"
                     /protein_id="HAY7367622.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JN749_001141"
                     /gene_synonym="wecD"
     CDS             complement(7463..8137)
                     /gene="rffC"
                     /locus_tag="JN749_001141"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HAY7367623.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN749_001142"
     CDS             complement(8115..8996)
                     /gene="rfbA"
                     /locus_tag="JN749_001142"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAY7367624.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN749_001143"
     CDS             complement(9015..10082)
                     /gene="rffG"
                     /locus_tag="JN749_001143"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAY7367625.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN749_001144"
     CDS             complement(10082..11344)
                     /gene="wecC"
                     /locus_tag="JN749_001144"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HAY7367626.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN749_001145"
     CDS             complement(11341..12471)
                     /gene="wecB"
                     /locus_tag="JN749_001145"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HAY7367627.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAVNYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN749_001146"
     CDS             complement(12527..13573)
                     /gene="wzzE"
                     /locus_tag="JN749_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HAY7367628.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN749_001147"
     CDS             complement(13585..14688)
                     /gene="wecA"
                     /locus_tag="JN749_001147"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HAY7367629.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN749_001148"
     CDS             complement(14928..16187)
                     /gene="rho"
                     /locus_tag="JN749_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAY7367630.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN749_001149"
     CDS             complement(16272..16373)
                     /gene="rhoL"
                     /locus_tag="JN749_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HAY7367631.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN749_001150"
     CDS             complement(16514..16843)
                     /gene="trxA"
                     /locus_tag="JN749_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HAY7367632.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            16974..18239
                     /gene="rhlB"
                     /locus_tag="JN749_001151"
     CDS             16974..18239
                     /gene="rhlB"
                     /locus_tag="JN749_001151"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HAY7367633.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            18373..19857
                     /gene="gppA"
                     /locus_tag="JN749_001152"
     CDS             18373..19857
                     /gene="gppA"
                     /locus_tag="JN749_001152"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HAY7367634.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN749_001153"
     CDS             complement(19904..21925)
                     /gene="rep"
                     /locus_tag="JN749_001153"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HAY7367635.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAVKRGK"
     gene            22012..22293
                     /gene="ppiC"
                     /locus_tag="JN749_001154"
     CDS             22012..22293
                     /gene="ppiC"
                     /locus_tag="JN749_001154"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HAY7367636.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JN749_001155"
     CDS             complement(22339..23814)
                     /gene="ilvC"
                     /locus_tag="JN749_001155"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HAY7367637.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            23964..24854
                     /gene="ilvY"
                     /locus_tag="JN749_001156"
     CDS             23964..24854
                     /gene="ilvY"
                     /locus_tag="JN749_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HAY7367638.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(24851..26395)
                     /gene="ilvA"
                     /locus_tag="JN749_001157"
     CDS             complement(24851..26395)
                     /gene="ilvA"
                     /locus_tag="JN749_001157"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HAY7367639.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(26398..28248)
                     /gene="ilvD"
                     /locus_tag="JN749_001158"
     CDS             complement(26398..28248)
                     /gene="ilvD"
                     /locus_tag="JN749_001158"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HAY7367640.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(28313..29242)
                     /gene="ilvE"
                     /locus_tag="JN749_001159"
     CDS             complement(28313..29242)
                     /gene="ilvE"
                     /locus_tag="JN749_001159"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HAY7367641.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(29262..29525)
                     /gene="ilvM"
                     /locus_tag="JN749_001160"
     CDS             complement(29262..29525)
                     /gene="ilvM"
                     /locus_tag="JN749_001160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HAY7367642.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(29522..31168)
                     /locus_tag="JN749_001161"
     CDS             complement(29522..31168)
                     /locus_tag="JN749_001161"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HAY7367643.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(31171..31221)
                     /locus_tag="JN749_001162"
     CDS             complement(31171..31221)
                     /locus_tag="JN749_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367644.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(31307..31405)
                     /gene="ilvL"
                     /locus_tag="JN749_001163"
     CDS             complement(31307..31405)
                     /gene="ilvL"
                     /locus_tag="JN749_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HAY7367645.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            31757..33277
                     /locus_tag="JN749_001164"
     CDS             31757..33277
                     /locus_tag="JN749_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HAY7367646.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(33302..33640)
                     /gene="maoP"
                     /locus_tag="JN749_001165"
     CDS             complement(33302..33640)
                     /gene="maoP"
                     /locus_tag="JN749_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HAY7367647.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            33759..34598
                     /gene="hdfR"
                     /locus_tag="JN749_001166"
     CDS             33759..34598
                     /gene="hdfR"
                     /locus_tag="JN749_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HAY7367648.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(34694..34769)
                     /locus_tag="JN749_001167"
     tRNA            complement(34694..34769)
                     /locus_tag="JN749_001167"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34734..34736),aa:Trp,seq:cca)
BASE COUNT         8480 a         9512 c         8781 g         8002 t
ORIGIN      
        1 tgtcacaact tctaataatg gggtggctaa tgggattcga acccacgaca actggaatca
       61 caatccaggg ctctaccaac tgagctatag ccaccactac aaatcttgtt acgcggtatt
      121 actaccaccg cagctcaagc gccgggacta aatggcgcgc ccgacaggat tcgaacctga
      181 gacctctgcc tccggagggc agcgctctat ccagctgagc tacgggcgct tagcgccgtt
      241 gcgggggtgg ataatacgga cttcccaccc ctctgtctag tcccttttta aataaattgc
      301 tcgtttggtt acggtttgtg cgttttgctg cttattcctc cagtttatgc gctttaccgt
      361 ggcgattaag cccaaaaact ttataaatcg ccgtcaccgc cagcataaag atgatgccga
      421 caaacagcga catacgcgta tcttcattaa agtacatgcc gatcaaaacg caaatcagga
      481 atgccattgt tatgtagttg gcccacggga acaggattga gcggaacgga tggctggcaa
      541 tcgccgcttt atgtgcacgc cgaaaacgca gctggcttat caatatcaca aaccatggca
      601 ccatccccgg aagcacgctg gcactgtaga cgtagacaaa cacacgctgc ggattgggaa
      661 tgatgtagtt caggcatgag ccaattagca gaatagcaat agatactgcc acacccgcaa
      721 ccggtacgcc gtgacgggaa acttttgcca ttgccgccgg taactgacgg tttttcgcca
      781 gtgcgtagag catacgtccg caactgtaca tgccgctgtt acagccagag agcgcagccg
      841 tcagcaccac aaagttgata atgcccgccg ctgcggtaat accgattttg gcaaaagtca
      901 gtacgaacgg gctgccgttg ctgccaattt cattccacgg gaagatggtg acgataacga
      961 aaatcgcgcc tacgtagaaa atcaggatcc gccacagcac tttgcctacg gcactgcgca
     1021 gcgtcacctg cggattcttc gcttcaccgg cagtaatgcc aatcagctcc acgccctggt
     1081 aggacgccac cacaatacac agagcggtca ggaacccttt ccagccaccc gcaaagaaac
     1141 cgccatgctc tgtgagattg ctaaaaccaa tcgactgccc gccattgcca aagccaaaga
     1201 aaatcacgcc caggccaatg acaatcatca cgataatcgt ggtgactttg atcatcgcga
     1261 accagaactc gatttcgccg tacaaccgca ccgccgccag attcgccaac gccaccagcg
     1321 ccaccgcgat caatgcgggt atccactgcg ccatctccgg ggaccaaaac tggacataaa
     1381 cgccaatggc ggtgatttct gagatcccca ccgccatcca cataaaccag taagaccagg
     1441 cggtgagata gccaaaaaac gggctcatat aacgatgcgc ataaacggcg aacgaaccgg
     1501 taaccggttc gaggaacaac atttcgccca ttgaacgcat gatgaaaaaa acgaacagcc
     1561 cggcgatgat ataggccaac aatacggatg gcccggccca tttcagggta ctggcggccc
     1621 ccataaacag gccgacgcca atggtgcccc ccagggcgat gagttcgata tgtcgagctt
     1681 ccagcccacg ctgtagctct ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat
     1741 gctttccggt gttaccggtt atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa
     1801 ttcaggagaa atggcaaata aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca
     1861 gacaggcgac gaagtgacca ctccgtcgct ttacaaagag aggaaaatca gaggttgccg
     1921 gtgtagtgcc agcgtaaata acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc
     1981 gaaagcaggc ggtagagcca ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt
     2041 acgtgaccgg tgaaaacatc gtaagtcccg ccaacgccca tatacagcgc atctggatgc
     2101 accagacggc agtcgcgcat gaagatctcc tgctttggcg atcccatcgc aacggtgacg
     2161 atttgcgctc cgctggcatg aatgcgttca aacagcgcct gacgctgctc gggtttaaaa
     2221 taaccatcct gactgccaac gatattcaca ttccattggt tgcgcagttt agcttcagtt
     2281 tgcgccagca cttcaggttt accgcccaca agaaataccg gcgtcccttc tttgcctgcg
     2341 cgcgccatca gctcttccca gagatcggca ccggcaacgc gggaaacctg cgcctgcggg
     2401 tactttttac gtactgaacg tacaacgctg atgccatccg catatttaaa ttcggcagcg
     2461 ttaattaact ccctgacctc ggcgttatct tcaatggtca gcattttttc ggcattaatg
     2521 gcaaccagcg ttccctgctt aagctgcccg tcagcaaaca gataatcgag ggcatgctgc
     2581 atatcacgcc aaccaatcaa ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg
     2641 ttattcattg ttatccttca acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga
     2701 gtccggcgct ttcaaaaagc cagtacaaca gttttgcgat catcagacat gcgccgaaga
     2761 ccacgataaa aaagactacg cgtgagacaa acgaatccag cccttcacgt gccagcacga
     2821 tcatattgaa gatcgcccca aagcagaaac tgtgcaatat cgcagcctta tagcgattag
     2881 tctcgcggtt gcccagctca tacagccagt cgaaccattt gatgatcagt ccaaccacaa
     2941 tcgccccgag tgggatgaac aacgcgccgc ccatcaccac cagtgagcct ataagcgtag
     3001 gcgagatcgc cagtccggag tggttattca gtacttccca ggtaaagtaa ttggctgagt
     3061 tcagcaccat actcgggcgc cccggccaca gccaggaagg gataaagaca tagaaatcgc
     3121 ggacaattgg agccaggccc tggaagtcga tgttgtcgta gttctgcaac agcaacgcca
     3181 gattctccca cggtgaaaag gtgtcgcgag tgagatagag aaacgtatag aacgcttcat
     3241 cgccgctcac attcattcca tagcgtttta acgccagcca gaacatgcca acaatcccca
     3301 gcacgcccgc cgccgccagc atccacaacg aaatccagcc gcgaataatg ccaataaaca
     3361 ggaagatagc gaatgcgatg atgatattgg cgcgagtgcc gccgacaatc atataagtca
     3421 gcaagccaaa ggcgaccgtg ctgacgagga aaaacagcca cgctttgctg tcctggcgca
     3481 gaaagtagac caccagcatc gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc
     3541 cggagacttc actggaaaag atctgactgt aggagttaag ccggaacagc aaaaagccgt
     3601 tgtgcatgaa gaagatgccg acgcttacca gcgcgatacc catcaggatc acccacgtaa
     3661 gattggtctc cacgcggttc atggtaaaca gcggacggcg cggtacatca gcaacgcgtt
     3721 tgcgtaggcg ggttttgtag gtgacatagt aaaccgcgta gaagcagccc gcagaaagca
     3781 acgcctgcaa caagatttct ggcggcgcga caccaacatc aaagcgaaat accagcacgc
     3841 tggtcagcgg gaagccgaag aaaaaggtga gcaaaaacag caatgaaaag aagacattga
     3901 agttaaagcg cacacggcga aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga
     3961 gcagccagac cacaaacagg ccactgaatt gcagcagact catgcgacct ccccggcggc
     4021 aatcgccaac gcccgctgcc agccttgtag atagttaggg ctaaagaagg caatggtgtt
     4081 tttatccgcc gacgccaact ggcgctgcgc ttcacgcaca atatcctcgt tgagatcgtc
     4141 ggtagtaaac agcactggca aatgctgttc cgtcatatcc tgccagaacg gattttccct
     4201 gttaagcaca caaggaatgc ccgcctgaat cagtaagcaa agcgtaccaa tgccctgctg
     4261 gcgggcaaaa ataaagtaac caagatcgca ctgacgaagt agcgccagat aggcgtcaaa
     4321 ttccagtttt tcgctcaaaa tttgcagatt ttcttcgctg aataactcca accccgcctg
     4381 acgaacttcc tcaatgtacg cttcgttatt aggcggatat cccatcggca ccaccacttt
     4441 taccgtatcg ccaaattgct gatgaacggc acgtaaagcg gcgacatgct cattgctgcg
     4501 gtcgccggag ttccccacca gaatggtcat ttttccttca cgttgccgat cgttcgccat
     4561 cgtattgaga gaagggtcca tccgcgtcgg gaagtacagc agttctcccc gcacctttgg
     4621 gtgcgttttg gcaaaaaagc tcaaatcgcc gcgggtggca aatacacagc cgactcgctt
     4681 ttgcgccagg cgacgtagtg ggtaaaaaag cttatatctc aagccactgg aaagctcata
     4741 caggtctgcc ccccagatat gccagaaaaa ctggctgggc ttaatgccac cactcagcag
     4801 agccagccac agtgtgggat tgaactgacc gtggaaaaaa aatcgctgct gacggttagc
     4861 ttttgctttc gcgatgaccg cttccgccag cgattttttc ccagggaaaa attgcacaga
     4921 aagcgccgga cagctatcac ttaagccgtc gtccttgcca acaaccataa actcgcacgc
     4981 gtgctcgctc gtcgcggcca gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg
     5041 agggatatcc gatcccagta cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa
     5101 aacacgccac aacaaagaga aaaatagacg atataagttg ccatatatgc ctgcgccgcg
     5161 cccagtgcac catgcgcagg gattagccag tgggcaaata ccatcaataa agtgaactgg
     5221 ctgacttccg ccagaatata aaaccgcagt gacgctttgg cgatcaccag ataaccaaag
     5281 acataagcgc ccacttttaa cacatcaccc actaactgcc aggcaaagag atcgcgcata
     5341 acggtaaatt tattcgacaa cagcaaccag atagcaaaat cacgcagcaa ccagacggta
     5401 aaactcgctg ccgccactgc cggtaagacg aatttcagcg atttaaccac ttcccgggtg
     5461 atatcgcgct tttccgttag ccgcgacaac gtgggcagca aatatacgct gaacgatgcc
     5521 gtaataaatt gcaggtaggc atcggaaata ctgctcaccc cttgccagat ccccacttca
     5581 tcccagctat actgcgccgc cagcagttta cgcatcatga tgtaagcaac aggcaaggtc
     5641 accgacgtaa tcaacgccat gagcgtaaat ttgctcaact gccctgccag accgttatcc
     5701 cagctgggtt tcagatagct taacgggatg acaccacgtt taatcaacat gatggcggca
     5761 ggaattacca ccagcgcggg aatcagcgcc agacccagca acgccccttc ataaccgcca
     5821 aaacggtaac tgacgtagta cgcgagaacg ccaatcaagc tgccgacaat cagcgataac
     5881 gcattacctg cggcatcgcg aaagcctttc atcagcgcca gtaacaggtt gccccaggcg
     5941 atccccattt gcaccagcgc cactaaacgc accagcccct gatagtcggt attaccaaac
     6001 aatccctggc tgattggcgc agctgccagc acaaaaacca gcgccatcag cgtagagaaa
     6061 ccaagtacca tcgctgatga agtgccgacc acgcggcgca gctgttgcgg attatcatgg
     6121 tactgggcaa cgtatttggt tacaccgtta aagatgccag ccccggcaag cacgccgagc
     6181 acggtaatca actggcggaa atttgccgcc agcccaagcc ccgccggacc aaatgacacc
     6241 gccagcaact taccgaccag taacccggca ccaatcttga ccagtgtact ggccgccgtc
     6301 cacaaggacg cttttgccaa cgacatatca ggagaagtag ttcaacaaag tcgcaattac
     6361 cgtacgctga ttgacgggcg acaggttgta gaacagcggc aggcgcagca ggcgctcgct
     6421 ctctttggtg gtgtagcgat cttcaccgtg gaactcacca aagtgttccc ccgcagggca
     6481 accgtgcagc ggaatgtaat gaaacaccgc catgatttcc gcttctttca gaaagttaat
     6541 caacgcgctc cggtcatcaa tatcccgcag tttaatgtag aacatatgcg cgttctgcac
     6601 gcagccatcg ggaatcgacg gcaactcgat acgcccggct ttcgccagag gcgctaacgt
     6661 atcgtagtag ttttgccaca gcgccagacg ttgctggttg atacgatccg ctgcttccag
     6721 ttgcgcccac aggtacgcag cttgcagatc ggacatcaaa tagctggagc caatatcgcg
     6781 ccaggtatat ttatcgacct gaccacggaa gaactggctg cggttagtgc ccttttcacg
     6841 gatgatctcg gctcgttcga ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc
     6901 acccgccgtg tagtttttgg tttcatggaa gctaaagcag ccaatatgac caatggttcc
     6961 cagtgcacgc cctttgtaag tggacatcac gccctgagcg gcatcttcca ccacaaacaa
     7021 attatgcttt ttcgccaacg ccataatggt gtccatttcg caggccacac ccgcgtaatg
     7081 gaccggcacg ataacgcgcg ttttgtcggt gatcgctgct tcaatcaacg tttcgtcgat
     7141 gttcatggtg tccgggcgaa catccacaaa gacgattttc gcgccacgca gcacaaaggc
     7201 attggcggtg gagacaaagg tgtagctcgg catgatcact tcatcgccag gctggatatc
     7261 gagcagcagc gccgccatct ccagcgaagc ggtgcaggac ggcgtcagta acactttggc
     7321 gctgccaaaa cgttgctcca gccactgctg gcagcgacgg gtaaaaccgc catcgccaca
     7381 cagtttgccg ctacccattg ccgactgcat atagtcgagt tcggttccca ccaccggcgg
     7441 tgcgttaaat ggaatcatgt gatcacctgt ataaccagta cgcggtgctt tctacattcg
     7501 caccactttg tatgtatcgt ttaagcgcgg cggtgttgcc catttgggtc gccacccgca
     7561 aagttgtttt accgcgagca tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg
     7621 caccgcgtcc agccagcagg ccaattcgcg catctgtcgc attgagttcc cgtaaagaga
     7681 catagccgcg aatatcgccg gacgccgcac gtagaatcag acattgatga tcaaaggtgc
     7741 cgcgcacggc attttcaatc cactgtgcat aaaagcgacc gctggcgtca ggcgcatacc
     7801 acggcgcacg aaaacggctt tgcgcaaatg cggcgctagc taactgacgt aatgcgggaa
     7861 tatcggtctc ttgtgccacg acagcaccgc tatcactgac attgttcacg ggtagcgcca
     7921 aatcaacttc accttctacc agggagaatc ccagctgttg cagggcatcc agttcccccg
     7981 tatttgatgc cgcaattttg gcctgcaccc gtgaccatgg cgctaaggcg tctgacgtca
     8041 ggagcggtgc ttcagacgta atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt
     8101 cccaggttag tggctcaata ctggcgcgga cgggcacgaa gtaactccag cagatattgg
     8161 ccgtagccag ttttcgctaa tgaactggca gcacgcttca caccctcgtc atcgagccag
     8221 ccgttacgcc aggcgatctc ttccaggcag gcaatcttaa agccctggcg tttttctact
     8281 gtctgcacaa aagtgctggc ctcaatcagg ctgtcgtgag tgccggtatc cagccaggca
     8341 aatccgcgcc cgagcagttc aacggtcagg ttgcccgcct cgaggtacat ctggttgatg
     8401 gaggtaatct ccagttcacc gcgctccgac ggcttcacct gctttgcgta ctccacgact
     8461 ttactgtcgt agaaataaag cccggtcacc gcccagtttg acttcggctg ttttggcttt
     8521 tcttcaagcg agatagcgcg gaaattgtcg tcaaattcca ccacgccaaa gcgttccggg
     8581 tccatcacct gatagtcaaa aaccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc
     8641 ttcggactga agccctgacc gaagaagatg ttatcgccca gcaccagaca agaaggttca
     8701 ccattaagga aggtttcacc gatgataaag gcctgcgcca gaccgtccgg gctgggctgt
     8761 tcggcatatt ccagctggat accgaactca ctaccatcgc ccagcaggcg ctggaaataa
     8821 cctttatctt ccggcgtagt gatgatgaga atttcgcgga taccggccag catcagcacc
     8881 gacagcggat agtaaatcat tggcttatcg taaatcggca acagttgctt cgatacgccg
     8941 cgcgtaatcg gatgcaatcg ggtaccggaa ccgcccgcca ggataatacc cttcatgttt
     9001 gcctccgctg aaaattagcc tttcagacct aaacgctcac cctgatagct gccgtcctgc
     9061 acctgcttcc accagctttc attagccaga taccactgaa ccgttttacg cattccactt
     9121 tcaaaggttt cctgcggcag ccagccaagt tcacgggcaa ttttcgaagc atcaatggca
     9181 taacgcagat catgccccgg acggtcagcg acaaaggtga tcaggtcacg atagtgcgcc
     9241 acgccgtgcg gcttgttcga agccagttct tccagcagct cgcaaatggt ttccacaaca
     9301 tcgagattct tacgctcgtt gtgaccacca atattatagg tttcaccgac tttcccggtg
     9361 gtcgccacgc aatacagcgc gcgggcgtga tcttccacat acagccagtc acggatttgc
     9421 tgcccgttgc catataccgg cagcggttta cccgccagcg cgttgaggat catcagcggg
     9481 atcagttttt ccggaaagtg gtaaggaccg tagttattcg agcagttggt gatcagcgtc
     9541 ggcagaccgt aggtccgcag ccaggcgcgc accagatggt cgctgctggc ttttgacgcg
     9601 gaataagggc tgctcggcgc atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc
     9661 aggtcaccat atacttcgtc ggtggagata tgatgaaaac ggaacgctga ttttttatct
     9721 tccgtcagcg tattccagta agcccgcgcc gcttcaagca atgtataagt cccgacaatg
     9781 ttggtttcaa taaacgctgc cgggccgtca atagaacggt caacatggct ttcggctgcc
     9841 agatgcatga cacagtctgg ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg
     9901 cagatatcaa ctttctcaaa ggcaaagcgc tcgctttgcg cgaccggtgc cagcgacatc
     9961 aggtttccgg cgtaggtcag cttatcgacc actaccaccg catcgctcgt ttcgttgatg
    10021 atataacgca ccagcgccga gccaataaac ccggcaccac ctgttatcag aatttttctc
    10081 atcagcgcca gactcctttg gcatcgacga catactgctg atggacattg tcgccattga
    10141 taactttgaa ctgactatga tcgaccagca tcaccagcac gtctgccgtt gccagcgcct
    10201 cgtcaagctg cgccagagta caaagcccgg tcagtttttt cggcaactgg tggatgttag
    10261 gctcaacaac cagcgtttcg ccgctgtgcc actgggcgat cagttcagcg atttccatcg
    10321 ccgggctttc gcgcaggtca tcaatattcg gtttaaacgc cagaccaaag caggcgattt
    10381 tcagttcact ggcgcgttta tcggtagcag caaggcagtc ggcaaccgcc gctttcacct
    10441 gatcgataac ccagaacggt ttgtgatcgt taacttcgcg cgcggtacgg ataagccgcg
    10501 cctgctgggg gttctgtgcc acgataaacc acggatcaac agcaatgcag tgaccgccca
    10561 cgccagggcc aggctgaaga atattaacgc gagggtgacg attcgccagg cgaatcagtt
    10621 cccagacgtt aatcccctga tcggcacaaa tcagcgacaa ttcattagca aaagcgatat
    10681 tcacatcgcg gaagctgttt tcggtgagct tacacatttc cgccgtccgc gagttagtga
    10741 cgacacactc accttcgagg aaaattttgt acaattcgct ggcgcgggcc gaacaaaccg
    10801 gcgtcatacc accaatcacg cgatcgtttt taatcagctc gaccataacc tggcccggta
    10861 atacacgttc cgggcagtaa gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga
    10921 aagtcagatc cgggcgcatc tctgctaacc attcagccat cttctcggtt gaccccaccg
    10981 gcgaggtgga ttcaaggatc accagcgcgc cttttttcag cactggcgca atggagcgag
    11041 cagccgattc aacgtaggtc atatctggct catgatcgcc tttaaacggc gtaggtacag
    11101 caatcagcca ggcatccgct tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta
    11161 cggcagtttt tactacgctc gccaaatcag gctcgacgat atggatttcg ccacgattga
    11221 tggtatcaac cgcgtgttgg ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa
    11281 acgctgctgc tgtaggcagc ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac
    11341 tcatagtgat atccgattat tttttaacgc ttccagaatg cgagagcatg cctgaccatc
    11401 accatacggg ttatgggcgc ggctcatagc ttgatattcg ttttcgtctt ttaaaagacg
    11461 cgtcacttcc tcgacaattc gctgcttatc cgtgcctacc agacgcaccg tacccgccgt
    11521 caccgcttcc ggacgctcag tggtatcgcg catcaccagc accggtttcc ccagcgaagg
    11581 cgcttcttcc tgaatgccgc ctgagtcagt caaaatcagc caggcgtggt tcatcagcca
    11641 gacaaacggt aaatactcct ggggatcgat cagaatgaca tttttcacat gccccagaat
    11701 gcgattgacc ggctctctga cgttcgggtt gagatgcacc ggatagacaa tctggatgtc
    11761 ctggtgcgtg gtggcgatgt ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa
    11821 actctcacgc ctatgaccgg tcaccagaat catcttttta tcgggggcga taaacgggta
    11881 atttaccgcc agttctgaac gcagcgtgtc gctgctcatc acctggtcac gcacccataa
    11941 cagtgcatca atgactgtat taccggtaat gaagattcgg ctatccgcaa cgttttcacg
    12001 cagcaagttt tgccgggaag tttcggttgg agagaagtga tacatcgcca gatgcccggt
    12061 caatgtacgg ttagcctctt ccggccacgg cgaatagaga tcgcccgtgc gcagaccagc
    12121 ctcaacgtga ccaacaggaa tacgctgata aaacgccgcc aggctggttg ccagcgtcgt
    12181 cgtcgtatcg ccgtgaacca gcacgacgtc tggtttgaac tcggcaagaa taggttttag
    12241 cccttccaga atccgacagg ttatctctgt caggccctgt cctggctgca ttatgttgag
    12301 atcgtagtca ggtacaatgg aaaagagttt cagtacctga tcaagcatct ctcgatgctg
    12361 cgcagtgacg caaactttag cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg
    12421 agccatcttg atggcttccg ggcgcgtacc aaatacagtc agtactttca catcgattct
    12481 cttcgaataa gcggcgagcg cctttgcgct caccgcagca gtgttgctat ttcgagcaac
    12541 ggcgggttaa tgcgacacca gccccgatca gccccccgac aatgccccac ataatcatca
    12601 ggaaggcacg acgtgggcta tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat
    12661 aggtctgaaa acgcggatcc agggttggac caacattcag ggtgtttaac atggctcgat
    12721 tctgatcata gtcgagatca aaggccggac cgacggcctg taaattttcc agtcgagcct
    12781 ggagcattgg acgcccaagc aggaacattt ctgaatcagg taattcctcg gcaggcacat
    12841 ctgtcgcact gcgcgaaata ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt
    12901 tcatccggcg gtcgtagatg gctttcgcca cctcttcctg acgcttcacc tgagctttca
    12961 tctggatggt gcgtgccgcc catgcgcctt tcagctcatc attcagatgg ctggctgcac
    13021 gctggctggc aaaagcaaca tactgacgta acaggttatt agcgtcaggc gcggtttcgg
    13081 caataagctt cacgctgtca ttgaccgcgc gagtaaagtc tccggggata aactggatgt
    13141 tgttaatcat ttcatccagc aacgccgcat cggctttgct gttgcccacc atccgctgtt
    13201 tgtaatagtc ggtttgcagc cagaactctc tgcgggtatc ccacgaggcc agttgcataa
    13261 caaactcttt ataggcttcg tccatgaccg atggttgatc ggcagaagcc atgtttgaac
    13321 ggacatccag gttacgcaaa aattgctgct gcgagtaata tccccccagc atattcaccg
    13381 ttggacgatc ggtaatcgcc gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca
    13441 gcgcgattaa cgcaaacgcc agccccatgc caataatcca tagcttccca gcccacaagg
    13501 tacgaaacaa cccacgaata tccagttcat tttcagcgtc ttcggccggt ttcccaggca
    13561 ttggttgtgt catcacatcc tcatttattt ggttaaattg gggctgccac cacgatttct
    13621 acgcagtctg cgttttacgc gcttaataaa gcgagcaact ttccaggcac gcttaatgca
    13681 atatccatag aggaagaatg ctagcaaaaa gagcaccagc atgacccact ccgggacaaa
    13741 atgagaatat tctgccagca cgccaatgga agcgagcagt gctgcggcaa gggtaatcag
    13801 cacaaacgcc tgacgggaag taaacccggc acgcatgatc aaatggtgaa tatgctgacg
    13861 gtcaggagag aatgggctca tgcctttacg caggcgacgg tacataatcg ccaccatatc
    13921 cattagcgga atggcgatta tccacaaagc ggtaaccggg ctgatgggat gggttttgcc
    13981 ctgggtcgtt tcgagcagga tccagataac ggtaaaacca atcagcgtac tgcccgcatc
    14041 acccataaag actttgtagc ggcgacccag gataccaagg ttaagcatga tgtatggcag
    14101 gatggcggcg atcatcgcaa agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa
    14161 aatcataccg attgctgcaa acgagacgca ggacaacccg cccagcaagc catcaatgcc
    14221 atcaaccatg ttgaacgcat taatggccgc ccagacggca aatagcgtca ggaagtaacc
    14281 aaacggtccg agcaccatct cccaggagcc aaagatataa cccaggctac tgagataaag
    14341 tttgccgaac accatcataa caatgccaac agcggcctgt atggtggcac ggatttttac
    14401 gctgatatca aaacgatcat ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag
    14461 atagagagat gcatgcggaa tatagtaatc gacaattccg aacgtgaagc aaatccctgc
    14521 gtaaaccgaa atccccccaa cgagaggtat caatccctgg tgacgtttgc ggaagtttgg
    14581 tttatccact aaaccgactt tttttgccac cttacgggca aaaaacagaa acagtgtcgt
    14641 gaataaaaaa atactgatga gatcagtact cactgtcagt aaattcacaa tgcatgctct
    14701 cagagaaaat tattagcaga agtataacca cgaagacctt tattcagaag ggaaagtccg
    14761 ttccgaaccc accagtcctg tgcaaaaatt aaccatttga tatgcaaatt gcataacatg
    14821 ccagcaaatt ccaggaattt tcctaacatc gccagcgcga cattaagatt acagatataa
    14881 aagcaaaacg ccacgtaaac acgtggcgtt tttggcataa gacaaattta tgagcgtttc
    14941 atcatttcga agaaatcgtc attggtcttg gtcatggcca gtttattaat gaggaattcc
    15001 attgcatcga tttcgcccat cgggtgaatg attttgcgca ggatccacat tttctgcagt
    15061 tcttcctgag tcgtgagcag ctcttcttta cgggtaccgg aacggttgta gtcgatagcc
    15121 gggaagacgc gtttttcagc gatcttacga gagaggtgca gttccatgtt gcctgtacct
    15181 ttaaactctt cgtaaataac ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg
    15241 ataatggtca ggctgccgcc ctcttccacg ttacgcgccg caccaaagaa gcgtttcgga
    15301 cgatgaaggg cattggcgtc cacaccaccg gtcaacactt tacctgacgc cggaacaaca
    15361 gtgttgtaag cgcgcgccag acgagtgatg gagtcgagca gaatgataac gtctttcttg
    15421 tgctcaacca ggcgtttggc cttctcgatc accatttccg caacctgaac gtggcgagat
    15481 gcgggttcgt caaaggtaga agcaacaact tcacctttta ccagacgctg catctcggtt
    15541 acttcttccg gacgttcgtc gatcagcaga accatcagca cacaatccgg gtggttgtaa
    15601 gcaatgctct gagcaatgtt ctgcagcagc atagttttac cggctttcgg cggtgccaca
    15661 atcagaccac gctgaccacg accgataggt gatgccagat ccagtacgcg agcagttaaa
    15721 tcttcagtag aaccgttacc acgttccata cgcagacgag agtttgcgtg cagcggggtt
    15781 aagttctcaa agaggatttt gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta
    15841 actttcagca gcgcaaaata gcgttcacct tctttcggcg ggcgaatctt accagagatg
    15901 gtatcaccag tgcggaggtt gaaacggcgg atttggctag gggaaacgta gatgtcatca
    15961 ggaccggcga ggtaggagct gtctgcggaa cggaggaaac caaatccatc ctgcaatatc
    16021 tccagtacgc catcaccaaa gatatcttcg ccactctttg cgtgctgctt caggatggca
    16081 aaaataatgt cctgcttacg catgcgagcc aggttttcca gccccatatt ttcgccgaga
    16141 gtgatcagct cagaaaccgg cgtattcttt aattcggtaa gattcatagt ggtgtgagtt
    16201 cttaaacttg gggtaaatct cgaacttaat gttgtgaatg gtatggcagg atcatccatg
    16261 cctgtttacg gtcatcgact catgtctttt cgctgcctgg tcacaggaga gtacgcagaa
    16321 ctgaaacgac aagacggatt gagtgacgag ccagaaatct gttcacttcg catttaagat
    16381 aaaacgggaa gcgttggaaa ttacaagatt caaacttaat aaggtatgtt taatacgaag
    16441 tcaacactaa gttagcatga ctcacgccgg gcgtccagtt tttagcgacg gggcacccga
    16501 acatgaaatt cccttacgcc aggttagcgt cgaggaactc tttcaactga cctttagaca
    16561 gtgcgcccac tttggttgcc gccacttcac cgtttttgaa cagcagcaga gtcgggatac
    16621 cacggatgcc atatttcggc gcagtgccag ggttttgatc gatgttcagt tttgcaacgg
    16681 tcagtttgcc ctgatattcg tcagcgattt catccagaat cggggcgatc attttgcacg
    16741 gaccgcacca ctctgcccag aaatcgacga ggatcgcccc gtccgctttg agtacatccg
    16801 tgtcaaaact gtcgtcagtc aggtgaataa ttttatcgct catatataac tccacaggaa
    16861 taagcctggc gtgttggttt cgttgttggt gtaacattaa ccaactaaag gttgacttta
    16921 tttcaccgga tacgctttcg taaagcaata gtaagctgat attctaccac actatgagca
    16981 aaacacattt aacagaacag aagttttccg acttcgccct gcatccgaag gttgtagaag
    17041 cccttgaaaa aaaagggttt cataactgta cgcccattca ggcactggcc cttccgctga
    17101 cgctggcggg tcgtgacgta gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt
    17161 ttcttacgtc aacgtttcat tatcttctct ctcatcctgc gattgccgat cgcaaggtga
    17221 atcaaccgcg tgccttaatt atggcaccga cgcgtgaact tgccgtgcag atccacgccg
    17281 acgcggaacc gctggcggaa gctaccggcc tgaagctggg cctggcttac ggtggtgatg
    17341 gctatgacaa acagctgaaa gtgctggaaa gcggcgttga cattctgatt ggcaccacgg
    17401 gtcgtttaat tgactacgcc aagcagaacc acattaacct cggtgccatt caggtagtgg
    17461 tactggacga agccgatcgc atgtacgatc taggctttat taaagatatc cgctggctgt
    17521 tccgccgtat gccgcctgca aatcagcgcc tcaacatgct gttctccgcc acgctttcgt
    17581 accgggtacg tgaactggcg ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac
    17641 cggaacagaa aacgggccac cgtattaaag aagagctttt ctacccttct aacgaagaaa
    17701 aaatgcgttt gctgcaaacg ctgatcgaag aagagtggcc agacagagcg attattttcg
    17761 ccaataccaa acaccgttgt gaagagatct ggggccacct ggcggcagat ggtcatcgtg
    17821 tcggtttgtt gacaggcgat gtcgcgcaga aaaaacgtct gcgtattctt gatgaattta
    17881 cccgtggcga tctggatatt ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc
    17941 cggcagtgac gcacgtcttt aactacgatt tacccgatga ctgtgaagat tacgttcacc
    18001 gtattggtcg tacaggtcgc gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag
    18061 agtatgcatt gaatttgcct gctattgaga cctatattgg tcactcaatt ccggtaagca
    18121 aatacaatcc ggacgcattg atgaccgatc tgccaaaacc gctgcgcctc acgcgcccgc
    18181 gcacaggcaa tggtccgcgt cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa
    18241 taaaatgtca tactcaaaat cattcaaact gtatcaagat ggcaagagaa tgaatcccga
    18301 tgagcttact gtagtaagtg attcgggtga aagaatgcag ccaacacaga gacagattga
    18361 aggatgaaga gtatgggttc cacctcgtcg ctgtatgcag ccattgatct cggttcgaat
    18421 agttttcata tgctggttgt gcgcgaggtg gctggaagca tccagacgct gacgcgaatt
    18481 aaacgcaaag tgcgtctggc tgctggcctg aatagcgaaa atgacctctc taatgaagca
    18541 atggagcgcg gttggcaatg tctgcgcctg tttgctgaac gtctgcaaga tatccctccc
    18601 tcgcaaattc gcgttgtcgc tacggcgaca ttacgacttg ccgtcaatgc gggtgatttt
    18661 attgccaaag cgcaggaaat cctcggttgt ccggtacagg tgatcagcgg tgaagaggaa
    18721 gcgcgtctga tttatcaggg cgttgctcac accactggtg gtgccgatca gcgcctggtg
    18781 gtggatatag gcggtgccag tactgaactg gtaaccggca cgggtgcaca aaccacctcg
    18841 ttgttcagcc tgtcgatggg ctgcgtcacc tggctggaac gctattttgc cgatcgtaat
    18901 ctggggcaag aaaattttga tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt
    18961 gccgatgaat tacggtatca cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag
    19021 gcgttacagg aaatcatgat ggcacagggg atggatgaac gcattaccct ggaaaagttg
    19081 cagcaattga aacagcgagc cattcattgc ggtcggctgg aggaactgga gattgacggg
    19141 ctgacgctgg aacgtgcgtt agtgttcccg agtggtctgg cgatcctgat cgccattttt
    19201 accgaactga atattcagtg tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc
    19261 tacggcatgt tgcatctggc cgtcgagcag gatattcgca gccgtacact gcgtaatatt
    19321 caacgccgct ttatgatcga tattgatcag gcacagcgcg tagccaaagt tgcggctaac
    19381 ttcttcgatc aggtggaaaa cgaatggcat cttgaagcaa taagccgcga tttgctcatc
    19441 agcgcctgtc agcttcatga aatcggcctg agcgttgact tcaaacaagc tccgcaacac
    19501 gctgcttatc tggtgcgtaa cctggatctt cccggtttta cccccgcaca gaagaaactg
    19561 ctggcgacgc tactgctcaa ccagactaac ccggtcgatc tctcatcgct gcatcagcaa
    19621 aatgccgtac caccgcgcgt cgcagaacaa ctctgccgtt tactccgcct ggctatcatt
    19681 tttgccagcc gtcgccgtga cgatctcgtg ccagagatga cattacaggc taaccatgaa
    19741 ctgttgacct tgacgcttcc gcaaggttgg ctaacccaac atccgctggg taaagagatt
    19801 attgctcagg aaaaccagtg gcagagctat gtccactggc cgctggaagt gcattaatga
    19861 gtaagtgccg gatgcgatgc tgacgcatct tctccggcct tgattatttc cctcgtttta
    19921 ccgccatcat cgctttcaga ttcgccagat ggctttgccc tttctgcatc cgttcttcgg
    19981 cactgaccac tttgcgctcc tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa
    20041 agcggctcgg ctccgggcgc accagttcgc cgtactgacg gcgttcttta cacagcgtaa
    20101 aggtcaattc cttctgggcg cgggtaatgc cgacataggc cagccgccgc tcctcatcga
    20161 tattatcttc atcgatactg ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt
    20221 agacataagg aaactccagc ccttttgacg catggagagt catcagttgc acctgatcca
    20281 gctcttcttc actctcacca cgctccatca tgtcgcgtaa agtaaagcgc gtcaccacct
    20341 gggtgagcgt catcggctca tccagttcgc tgccttccag catctccgtc atccagctaa
    20401 acagttggtt gacgttcttc atgcgcattt cggcagcttt cgggctgggc gatgtttcgt
    20461 acagccagga ttcataatcc atgccgtgga tcagatcgcg caccgcggca atcggctccc
    20521 gctccgccag acgctggatt tctgccaacc agtgagtgaa gcgggtcaat gcttcataac
    20581 cacgtccgct aagcgtctga ctcaggccca tatcaaagct ggcggtaaac atgcttttat
    20641 tgcgcgtcat cgcccactca cccagctttt tcagcgtcgc cgggccaatc tctcgcttcg
    20701 gcgtgttaac gatacgcaga aatgcgctgt catcgtccgg gttagtcagc acgcgcagat
    20761 aagccagcaa gtccttgatt tcaggacgag agaaaaacga cgtaccacca gaaattttgt
    20821 acgggatgcg gttttgcatc aggaattttt caaacacccg cgactgatgg ttaccgcgat
    20881 aaagaatagc gtaatctttg tactgcgttt tattgacgaa gtgatgcgca atcagttcac
    20941 cagtgacgcg ctcggcttca tgttcttcgt tattcgcgct taaaactttt agctctgtgc
    21001 cgtaacccag ttcggagaac agacgctttt caaagacgtg cgggttattg gcgatcagga
    21061 tgttcgccgc tttcaaaata cgtccggaag agcggtagtt ctgctcaagc ttaatcacct
    21121 tcagcgccgg aaaatcctgg ctcagcagca ccaggttttg cggacgtgcg ccgcgccagg
    21181 agtagatcga ctggtcatcg tcacccacca cggtaaagcg cgcgcggctg cccaccagca
    21241 gtttcaccag ttcatactgg ctggtgttgg tgtcctgata ttcgtccacc agcaggtagc
    21301 gaattttgtt ctgccagcgc tcgcggactt cttcattgcg ttgcagaagt agcgtcggca
    21361 ataaaatcag atcatcgaag tcgagcacat tacaggcttt caggtgtgca tcatacagcc
    21421 cataacaatg ggcaaaaata cggtcccgct cgccaatcgc actggctgcc gcctgggacg
    21481 gtgttttgag atcgttcttc cagttagaga tggtcgaaat caactgttgc aggagaactt
    21541 tgtcatcttc aatcagccct tcggtcaact ctttgagcaa cgccagctga tcggtatcgt
    21601 caaacagcga aaagttcgcc ttcatcccca gcgccgcata ctcacgcttg atgatatcca
    21661 gccccagcgt gtggaaggtt gagatcatca gcccacgcgc ctctttgcga cccagcgtct
    21721 gtccgacacg ctctttcatc tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat
    21781 gtcgcgcctg ataaccgcac ccgcggatca gatgggcgat tttattggtg ataacacgag
    21841 ttttaccgga acccgcaccc gccagcacca ggcaggggcc ggtaacgaat tcgacagctt
    21901 gttgttggcc ggggtttaga cgcataggaa aatcgctcaa tgaaagtcgg gaggggaaaa
    21961 taacagcgtg gtagtatagc cagcctcatc cttaccactc aaggcacgat catggcaaaa
    22021 acagcagcag cactgcatat ccttgtaaaa gaagagaaac tggctctgga tcttctcgtg
    22081 cagattaaga acggtgccga tttcggcaag ctggcgaaga aacactccat ttgcccatca
    22141 ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc agatggttcc ggcgttcgat
    22201 aaagttgtgt tctcttgtcc ggtactggag ccaaccggcc cgctgcacac ccagttcggt
    22261 taccacatca ttaaggtgct gtaccgtaac taatagcaag gccttctccg ggagaaggcc
    22321 ttgaactctg aatcgctatt aacccgcaac agcaatacgt ttcatatccg tcatatagcc
    22381 gcgcagtttc ttacctactt gctcaatcgc atggctgcga atcgcttcat ttacatcacg
    22441 cagctgcgcg ttatctaccg caccttccgg aatagcttta cccaaatcgc ccggttgcag
    22501 ttctgccata aacggtttca gcagcggcac acaagcgtaa gagaacagat agttaccgta
    22561 ctcggcagta tcagagataa ccacgttcat ttcatacaga cgcttacggg cgatggtgtt
    22621 ggcaatcagc ggcagttcgt gcagtgattc atagtaagca gattcttcga tgatgccgga
    22681 atcaaccatg gtttcgaacg ccagttcaac gcccgctttc accatcgcga tcatcagtac
    22741 gcctttatcg aagtactcct gctcgccgat tttgccttca tactgcggcg cggtttcgaa
    22801 ggcggttttg ccggtctctt cacgccaggt cagcagtttc ttatcatcgt tggcccagtc
    22861 cgccatcata ccggaggaga attctccgga gatgatgtcg tccatatgtt tctggaacag
    22921 cggtgccatg atctctttca gctgttcaga caacgcgtaa gcacgcagtt tcgccgggtt
    22981 agagagacgg tccatcatca gtgtgatacc gccctgcttc agcgcttcgg tgatggtttc
    23041 ccaaccgaac tgaattagtt tttctgcgta tgccggatcg gtgccctctt ccaccagctt
    23101 gtcgaagcac agcagcgagc cagcctgcaa cataccgcac aggatggttt gctcacccat
    23161 caggtcagat ttcacttccg caacgaagga cgattccagc acgcccgcac ggtgaccacc
    23221 ggttgctgcc gcccatgctt tagcgatcgc catgccttcg cctttcggat cgttttccgg
    23281 gtgaacggca atcagcgtcg gtacgccgaa tccacgtttg tactcttcac gcacttcggt
    23341 gccagggcat ttcggcgcaa ccatgactac ggtgatgtct ttacggatct gctcgcccac
    23401 ttcgacgatg ttgaaaccgt gcgagtaacc cagcgccgcg ccgtctttca tcagtggctg
    23461 tacggtgcgc actacatcag agtgctgctt gtccggcgtc aggttaacca ccagatccgc
    23521 ctgtgggatc agttcttcgt aagtacccac tttaaaacca ttttcggtcg ctttacgcca
    23581 ggacgcgcgc ttctcggcaa tcgcttcttt acgcagagcg taggagatat cgagaccaga
    23641 atcacgcatg ttcagaccct ggttcagacc ttgtgcgcca cagccgacga tgactacttt
    23701 tttaccctga aggtagctcg cgccatcggc gaattcatcg cgccccataa agcgacattt
    23761 gcccagctgt gccagctgct gacgcagatt cagtgtattg aagtagttag ccatggtgat
    23821 tcctcgtggt gttgtgcttc ttattgttcg gttcgcgttt gcgagattga attcactata
    23881 tgacaggaaa tttattgcgg aaattgatat attcacaacg tcacattgca atttctgcaa
    23941 cgtcaacatt gagggctgcg ccattggatt tacgcgatct gaaaaccttc ctgcatctgg
    24001 cggaaagccg ccattttggc cgcagcgcgc gggcgatgca cgttagcccg tccacgctct
    24061 cacggcagat tcagcgcctg gaagaagatc tcggccagcc gctgtttgtg cgcgataacc
    24121 gcacggtgac gctgactgaa gcgggcgaag aactgcgcgt tttcgcccag caaacgctgt
    24181 tgcagtatca gcagctgcgc cacaccatcg accagcaagg gccgttgctc tatggcgaat
    24241 tacatatctt ctgctcggtg accgccgctt acagccatct gccgccgatt ctggatcgct
    24301 tccgcgcgga gcacccgtcg gtggagatca aactcactac cggtgatgcg gcagatgcaa
    24361 tggaaaaagt cgtcacaggt gaagctgatc tggctattgc cgggaagcca gagactctgc
    24421 ccggcgcggt ggcgttttcg atgctggaga atctggcagt ggtgttgatt gcacccgcat
    24481 tgccctgccc ggtgcgtaat caggtttctg tcgaaaagcc ggactggtca acggtaccgt
    24541 ttatcatggc cgatcagggg ccggtgcgcc gccgcattga gttgtggttc cgacgcaata
    24601 aaatcagtaa cccgatgatt tacgccacgg ttggcgggca tgaagcgatg gtatcgatgg
    24661 tggcgctcgg ctgtggcgtg gcattgttgc cggaagtggt gctggaaaac agccccgaac
    24721 cggtgcgtaa ccgcgtgatg attttagagc gcagcgatga gaaaacgccg tttgagctgg
    24781 gcgtgtgtgc gcagaaaaag cggctacatg agccgctaat tgaggcattc tggaagattt
    24841 tgccgaacca ttaacccgcc aaaaagaacc tgaacgccgg gttattggtt tcgtcgtggc
    24901 aatcgtagcc cagctcattc agccgggttt cgaaatccgg ttcatggtcg ccaagttcga
    24961 acgccgccag tacgcgcccg tagtcggtgc catggctgcg atagtggaac aaagaaatgt
    25021 tccagtgcgt acccagcgtg ttgaggaagc gcagcagcgc gcccggtgat tccgggaatt
    25081 cgaagctgta gaggcgttcc tgcaacggat gtgacggacg cccgccgacc atatagcgca
    25141 catgcaactt cgccatttcg tcgtcggaga gatccaccac gctgtagccg ccgtcgttga
    25201 gcatctgcaa aatttctttg cgctcttcga ggccgcggct caggcgcacg ccgacaaaga
    25261 tgcaggcgtt tttggcatcg gcaaaacggt agttgaactc ggtgaccgat cgcccgccaa
    25321 gcagttggca gaatttgagg aagctgcctt tttcttccgg aatggtcacc gccaacaacg
    25381 cttcacgctg ttcgcccagt tcgcagcgtt ccgagacgta gcgcaggccg tggaagttca
    25441 cgttggcacc ggaaagaata tgcgccagcc gttcgccgcg aatgttgtgc tgggcgatat
    25501 attttttcat tcccgccagc gccagcgcgc cagagggttc cgccaccgca cgcacatctt
    25561 cgaataaatc cttcatcgcc gcacagatcg catcgctatc gacggtgatg atgtcgtcga
    25621 gatactcctg gcataaacgg aaggtttcgt caccgatgcg ttttaccgcg acaccttcgg
    25681 cgaacagtcc cacgcgtggc agatcaaccg gatgacccgc atccagcgct gctttcaggc
    25741 aggcggagtc ttccgcttct acggcgatca ctttgatttg cggcatcagt tgtttgatca
    25801 gcaccgctac gcccgccgcc agaccgccgc cgccgactgg cacaaatacg cggtcgagat
    25861 gggagtcctg ctggagcagt tccagcgcca gcgtgccttg cccggcaatc accatcggat
    25921 ggtcgaacgg cggcacccag gtgaacccct gctgctgtga cagttcgatc gctttagctt
    25981 tcgcttcgtc aaagttcgcg ccatgaagca gcacttcgcc gccgaatcca cgtaccgcat
    26041 cgactttgat gtcggcggtg gcggttggca taacgatcag ggccttcacg cctaaccgcg
    26101 cagaagaaaa cgcgacgccc tgcgcgtggt tacccgcaga agcagtgatc acgccgtgcg
    26161 ctttttgttc ttccgtcagg cccgccatca tggcgtatgc gccgcgcagc ttaaagctgt
    26221 gcactggctg gcgatcttcg cgcttcacca gaatgacgtt atcaagacgc gacgacagtt
    26281 tttccatttt ttgtagcggc gtaacctgcg ccgcctcgta aaccggcgcg cgcagcactg
    26341 ctcttaaata ttcggcacct tccggagcac cggacagggg ttgcgagtca gccattatta
    26401 accccccagt ttcgatttat cgcgcaccgc acctttgtcg gcgctggttg ccaggctggc
    26461 gtaggcgcgc agggcaaagg aaacctgacg ttcacggttt ttcggcgtcc aggctttgtc
    26521 accccgagct tcctgcgctt cacgacgcgc cgccagttcg gcatcgctta cctgtaactg
    26581 aatgccacgg ttcgggatgt cgatagcgat caggtcacca tcttcaatca gaccaatgct
    26641 gccgccgctt gccgcttccg gtgagacgtg gccgatggaa agaccagagg tgccgccaga
    26701 gaaacgaccg tcggtgatca gcgcacaggc tttaccgaga cccattgatt tcaggaagct
    26761 ggttgggtag agcatttcct gcatgccagg gccgcctttc gggccttcat agcggatcac
    26821 caccacatcg cccgcgacaa ctttgccacc gaggatcgct tctaccgcgt cgtcctggct
    26881 ttcgtacact ttcgccgggc cggtgaattt gaggatgctg tcatcgacgc ccgcggtttt
    26941 cacgatgcag ccgttttcag caaaattacc gtagagcacc gccaggccgc cgtctttgct
    27001 gtaggcgtgt tccagcgagc ggatacagcc gtttgcgcga tcgtcatcga gagaatccca
    27061 acggcaatcc tgcgagaatg cctgtgtagt acgaatgccc gcagggcctg cgcggaacat
    27121 attttttacc gcgtcatcct gggtcagcat aacgtcgtat tgttccagag tttgcggcaa
    27181 cgtcaggcca agtacgtttt tcacatcacg gttcagtaac cccgcgcgat ccagttcacc
    27241 gagaataccg ataacaccac cagcacggtg aacatcttcc atatggtatt tttgggtgct
    27301 cggcgcaact ttgcataact gcgggacttt acgcgaaagt ttgtcgatat cactcatggt
    27361 gaagtcgatt tccgcttcct gcgccgccgc cagcaggtga agtaccgtgt tggtagagcc
    27421 gcccatcgcg atatccagcg tcatagcatt ttcaaacgcc gctttgctag caatattgcg
    27481 cggcagtgca ctttcgtcgt tttgctcgta gtaacgtttg gtcaattcaa caatgcgttt
    27541 accggcattg aggaacaact gcttacggtc cgcgtgggtt gccagcagcg agccgttgcc
    27601 cggctgcgac aggcccagcg cttcggtcag gcagttcatt gagttagcgg taaacatccc
    27661 ggagcaggag ccgcaggtcg gacacgcgga acgttcaacc tgatcgctct gggagtcaga
    27721 aactttcggg tctgcgccct ggatcatcgc atcaaccaga tcgagtttga ttatctgatc
    27781 ggacagtttg gttttcccgg cctccatcgg gccgccggaa acaaagatca ccggaatatt
    27841 caggcgcagg gaagccatca gcatccccgg ggtgattttg tcgcagttag agatgcagac
    27901 catggcgtcg gcgcagtggg cgttgaccat atactcaacg gaatcagcga tcagttcgcg
    27961 agatggaagt gaataaagca tccccccgtg gcccatggca atcccatcat ccaccgcaat
    28021 ggtgttgaac tctttggcaa cgccgccagc cgcttcaatt tgttcggcga ccagtttacc
    28081 gagatcgcgc agatggacgt gacccggtac aaattgggtg aacgagttca caaccgcgat
    28141 aatcggcttg ccaaaatcgg cgtcggtcat tccggtggcg cgccacagcg cacgagcacc
    28201 cgccatatta cgaccatgag tggtggtggc ggaacggtac ttaggcatac tttatttact
    28261 cccagtgtct gtctcgtaaa tgggacggtg cgtgccgtcc cattttttgt atttattgat
    28321 taacttgatc taaccagccc catttatctt cggtttcgcc agtgaagagg ccgaagaagg
    28381 cttgctgaat gcgtttggta accgggccac aacggccttc gccaatctga ataccgtcta
    28441 cgctgcgcac tggcgtgatt tctgccgccg taccggacat aaagacttca tccgccaagt
    28501 acagggattc gcgcgacagc acctgctcac gcacttcaat tcccagctct ttcgccagtt
    28561 tgatgatggc atcacgggta atacccggca gcgcggagga ggtgaacggt ggggtgaaca
    28621 gcacaccatc tttcacttca aacaggtttt cgcctgcgcc ttcagagata taaccgttca
    28681 catccagcgc gataccttcc tgataaccgt ggcggcgcgc ttcgctaccc accagcaggg
    28741 aagagagata gttaccaccg gcttttgccg cagttgggat ggtgtttggt gctgcgcggt
    28801 tccaggagga aaccatcgca tcgatcccct gctccagcgc ttctgcgccc agatacgctc
    28861 cccacgggaa agcggcgata atcacatcgg ttgagtatcc cgctggcggg tttactccca
    28921 tgccaacatc accgacgaag atcagcggac ggatataggc gctggtgaga ttgtttttgc
    28981 ggatcacgtc acgacaagct tccatcagct catcaatgct ctgtgaaacc gggaagcgat
    29041 agattttggc ggagtcatgc agacgctgca tatgctcacg atggcggaat acaaccggtc
    29101 ctttgtgtga gtcgtagcaa cggatgcctt caaaaaccga ggtgccatag tgcagcgcgt
    29161 gcgacatcac atgcaccttc gcgtcttccc agcgaaccat ctccccattg aaccaaatgt
    29221 aatcagcttt cttcgtggtc atttttatat tccttttgcg ttcaggcgcg gatttgttgt
    29281 gatgtggttg tgctctggca gatggcaacg tgtgcgacgt ccaccagttt atttaactga
    29341 ctaaacagta agtcgaccga ccgtgggctg gcaacggtca attcgatatt tatattttgt
    29401 gcatcgctgg cggcagccat attcattgag cagacgtgga aaccacgatg acgcaccacg
    29461 cgtaaaacac gttctaaggt ttccggattg aagcgagccg atacattgac ctgatgttgc
    29521 atcatgataa tttctccaac atttctgaat tactggcacc tggcggcacc agcggccaga
    29581 cgttctcaag ttcgtcgatt gagacatgaa gcaggtatgg cccatcactg ttcagcatgg
    29641 tgtcgagtgc cgcttcaacc tggtctttac gggtgatgtg ttggccaggg atgccgaagg
    29701 cgctggctaa catgaggaaa tcggggttat cggtaagggt ggtttcgctg tatcgttcct
    29761 gaaaaaacag ttgctgccat tgtcgaacca tccctaaccg ttggttatcg agtaagacga
    29821 ttttcaacgg taactgcttg cgttttacgg tgcccagctc ttgcacattc atcatgaaag
    29881 agccgtcacc ggagatacag acaacggtat cgttcggtcg cgcgacttgt gcgccaactg
    29941 ccgccggtaa accaaaaccc atggtaccta agccgctgga ggtgatgaaa ttttccgggc
    30001 gagtgtgggc gatgtgctgc gcagcccaca tctggtgctg ccccacatct gtggtcacga
    30061 cgcaatccgc aggtttacga tccgacagtt gttttaacaa caacggagcg tagatagcgt
    30121 caccgggatg gtcgtaacgc caggaatgtt catcacgcag ctgcgcgcag tgttgctgcc
    30181 agtcattgat atttaacggc tgctgtaatg ctggtaacag agcatttaaa tcaccttgta
    30241 atgccacatg tgcctgacgc agcttgttca tttctgccgg gtcgatatcc atatggataa
    30301 cactggcgtg tggtgcgaag gtgttcagtt tgccggtcac ccggtcatca aaacgtgcac
    30361 ccacggcgat cagcaggtca cactcctgca ccgcgaagtt tgccgctttg gttccgtgca
    30421 tccccagcat gcccagatag tacggataat ctgcttctac tgcgcccagc cctttcagcg
    30481 tacaggtggc aggcattttt gtggcagcga gaaattcacg caaagccgga actgcctgcg
    30541 ccatccccac gccaccgcca acgtacagca tcggtttttg cgcttttgcc agcatctggc
    30601 gcgcttgctc gacttcggca tgtgggaaag tcacttcgtt ttcaacggtg gtgaaccacg
    30661 gttccagatc gccgctggct aactggatat cttttgggat atcgaccaga accggaccag
    30721 gacgacctga gctggcaacg tcgaatgctt cagccatgat gcgcggcaac tcttccagag
    30781 actgcaccag aaagctgtgc ttggtacagg ctaacgacaa tcccaggaca tccacttcct
    30841 gaaatgcgtc ggtgccgata aacggtgcgg acacctgacc agtgatggca acgacaggga
    30901 tggaatctaa cagtgcgtcc gcaagcccgg ttatcaggtt ggttgcgccc ggaccagacg
    30961 tggcgataca tacgccagtt ttaccggtag cacgggcata accgatagcc gctattgccg
    31021 caccctgctc atgtcggcac agcaagtgct ccacgccgcc gtcatacaat gcatcgtaaa
    31081 ccggcataat tgcgccaccc ggataaccga aaacggtgtt cacaccctgt gcccgcaacg
    31141 catgtaccac ccactgtgcg ccattcatag ttagttcccc gtcctgaatc ttgagaaaca
    31201 gaattttgtg ctgttattca ttgtctgctc ctcggttatg tttttaaggt caaaaaaacc
    31261 cccggacctt tcggtgcggg ggtcttagtt cgttaaggct tgatctctaa gcctttcctc
    31321 gtccaagtgc agccccgcac ggtgggataa taatcaccac cacgctaatc acgaccaggc
    31381 taatcactcg tagaagggct gtcattttgt cttttcttgc atcttgttcg aaggaatgcc
    31441 taaagagtta ccatagattt tgcgaatagc acaagatatt tttgggccta tttttatgat
    31501 ggctttaata atttcaatag aaattgttgt tttttaaaaa gaaaatagtg agtgaaaaaa
    31561 ttcaatttca gtataaatgt tgttcccttc ctcgtagttc atcctttgtc cgctgcattt
    31621 ttgcgagcat cattccaccg ccagaacaac tcaacttcac ttgcacaaat aaacaggaat
    31681 cagccactaa aattcacgaa ttagcgggct acagcagcga tatcttatgc acataatggt
    31741 gactcaagga gggtttatgt cactgtcaat tgttcatacc cgcgcagccc tggaagtaaa
    31801 tgcgccccca atcactgttg aggtacatat cagtaaaggt ctacccggct taacgatggt
    31861 gggcttaaca gaaacaacgg tgaaagaagc tcgcgatcgc gtgcgcagcg ccattatcaa
    31921 tagcggatat gaatatccgg cgaaaaaaat caccatcaac cttgctccag ccgatctgcc
    31981 aaaagaaggg ggacgatatg atttacctat cgccattgcg ttgctggcgg cctcagaaca
    32041 gcttacagcc aataagttag atgaatatga attagtcgga gaactagcgc ttacaggcgc
    32101 tctgcgtggc gttcccggtg caatctccag tgcaactgaa gctattaagt cgggcagaaa
    32161 aattatcgtc gcgaaagata acgaagatga agtggggcta attaacggtg aaggatgcct
    32221 gatagccgat catctgcagg ctgtctgtgc gtttctggaa ggtaagaaca ctctcgaacg
    32281 cccgaaacca actgatgcag tatcccgggc gctacaacat gatctcagtg atgttgtcgg
    32341 tcaggagcaa ggaaaacgag gactggaaat taccgccgct ggtgggcaca accttttact
    32401 gattgggccg ccgggaacag gtaaaacaat gctcgccagc cgtattaatg gcctgttgcc
    32461 agatttaagc aatgaagagg cactggagag cgctgcgata ttaagtctgg taaatgctga
    32521 atcagtacaa aaacaatggc ggcagcgccc gttccgctca cctcatcaca gtgcgtcgtt
    32581 aactgcgatg gtgggcggtg gtgcaattcc agggccaggt gaaatttcgt tggcgcataa
    32641 cggcgtgctt tttcttgatg agctacctga atttgaacgg cgtacactgg atgccttgcg
    32701 agagccgatt gaatccgggc agatccatct ttcacgcaca cgagcaaaaa taacctatcc
    32761 agcccgtttc cagcttgttg cggcgatgaa tcccagccct accggacatt atcagggaaa
    32821 ccataaccgc tgcacgccag aacagacatt acgttatctc aaccggctct cggggccctt
    32881 tctcgaccgc ttcgatctct cactggagat cccattacca ccccccggca ttttgagtaa
    32941 aacggtagtg ccgggagaaa gcagcgccac cgttaaacaa cgcgtaatgg ccgccagaga
    33001 gcgccaattt aagcggcaga ataaactgaa tgcctggctg gatagtccgg aaatacgcca
    33061 attctgcaaa cttgagagcg aagatgcgat gtggttggaa gaaacgctga tccatctggg
    33121 gttatcgatt cgtgcctggc agcggttact gaaagttgca cgaaccattg ctgatattga
    33181 tcagtctgac attatcacac gtcagcattt gcaggaggca gttagctatc gtgcgattga
    33241 ccgtttgctc atccatctgc aaaaactact gacataaaaa aagggcattt cgcccttttt
    33301 attaatcgtc agaatcggtg tagtcttcag caccttcaac ctgcggttta ccgccggaaa
    33361 gggtgtgaaa acgttttgga cgcttaatac gcgtcatata cttggaccac acgcgttctg
    33421 cttctgtcac tggctcacgt tcgccacggc atactgctac gaagagtttc tcttcctcgg
    33481 taaccggctc gcgtttgcca agatccaact cattgaaggc ataaccatga cgctcaagca
    33541 gttgtgcctc tttgatggtg aaatcaccat gacgagagaa tccacgtgga taatgtttat
    33601 tgtcgaaata tcgattagtc gtcgtaaagc tttccgccat cctgcacgct cctaattctt
    33661 tgaccgagct agttatggcg cggagtatta gttacgcttg acagagtgta aaacaaaaca
    33721 tttaaatcat aacgacaaat aattttgcgg agagcactgt ggatacggaa ttgttaaaaa
    33781 ctttcctgga agttagccga acgcgtcact ttggtcgagc ggctgaatcg ctctatctga
    33841 cccagtcagc agtgagcttt cgaatcagac aactggaaaa tcaactgggt gtgaaccttt
    33901 tcacccgcca cagaaacaat atccgtttaa ccgctgccgg tgaaaaacta ctgccttatg
    33961 cagaaacgct catgagcacc tggcaggccg cccgcaagga ggtggcgcat acctcacgac
    34021 ataacgagtt ttctatcggt gccagcgcct cgttgtggga atgtatgctt aatcagtggt
    34081 tgggacgctt gtatcaaaat caggatgccc atacaggctt acagttcgaa gcgcgaattg
    34141 cccaacggca gtctctggta aaacagctgc atgaacgcca gcttgatctt cttattacca
    34201 ctgaagcgcc caaaatggac gaatttagta gtcagttgct ggggtatttc actttagcgc
    34261 tttataccag tgccccttct aaactaaagg gagatcttaa ctatctgcga ctggagtggg
    34321 ggccagattt tcaacagcat gaggcaggtt tgatcggtgc tgacgaagtt cccattctga
    34381 caaccagttc tgctgaactg gcacagcaac agattgcgat gcttaatggt tgcacctggc
    34441 tacccgtcag ctgggcgcgt aaaaaaggcg gcctgcatac cgttgtcgat agcacaacac
    34501 tttcacggcc gctttatgcc atatggctgc aaaatagcga taaaaatgcg ttgattcgcg
    34561 atcttttgaa aattaacgtg ctggatgaag tgtattaatc tgaatggctg gcaaggatgc
    34621 cggtagaagg atttacttcg gagaggatta tttcagataa aaaaaatcct tagctttcgc
    34681 taaggatgat ttctggcagg ggcggagaga ctcgaactcc caacacccgg ttttggagac
    34741 cggtgctcta ccaattgaac tacgccccta attag
//
LOCUS       DADLTF010000025        34275 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.025, whole genome shotgun sequence.
ACCESSION   DADLTF010000025 DADLTF010000000
VERSION     DADLTF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.025"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(94..2190)
                     /locus_tag="JN749_001168"
     CDS             complement(94..2190)
                     /locus_tag="JN749_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7367649.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            complement(2192..2443)
                     /locus_tag="JN749_001169"
     CDS             complement(2192..2443)
                     /locus_tag="JN749_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7367650.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            2596..3159
                     /locus_tag="JN749_001170"
     CDS             2596..3159
                     /locus_tag="JN749_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage CI repressor"
                     /protein_id="HAY7367651.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            complement(3435..3857)
                     /locus_tag="JN749_001171"
                     /pseudo
     CDS             complement(3435..3857)
                     /locus_tag="JN749_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(3857..4450)
                     /locus_tag="JN749_001172"
     CDS             complement(3857..4450)
                     /locus_tag="JN749_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAY7367652.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            4597..5004
                     /locus_tag="JN749_001173"
     CDS             4597..5004
                     /locus_tag="JN749_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAY7367653.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="JN749_001174"
     CDS             complement(5124..6116)
                     /gene="rpoS"
                     /locus_tag="JN749_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HAY7367654.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="JN749_001175"
     CDS             complement(6179..7318)
                     /gene="nlpD"
                     /locus_tag="JN749_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HAY7367655.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKIGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="JN749_001176"
     CDS             complement(7458..8084)
                     /gene="pcm"
                     /locus_tag="JN749_001176"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HAY7367656.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(8078..8839)
                     /gene="surE"
                     /locus_tag="JN749_001177"
     CDS             complement(8078..8839)
                     /gene="surE"
                     /locus_tag="JN749_001177"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HAY7367657.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(8820..9869)
                     /gene="truD"
                     /locus_tag="JN749_001178"
     CDS             complement(8820..9869)
                     /gene="truD"
                     /locus_tag="JN749_001178"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HAY7367658.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICAKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="JN749_001179"
     CDS             complement(9866..10345)
                     /gene="ispF"
                     /locus_tag="JN749_001179"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HAY7367659.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="JN749_001180"
     CDS             complement(10345..11055)
                     /gene="ispD"
                     /locus_tag="JN749_001180"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HAY7367660.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="JN749_001181"
     CDS             complement(11074..11385)
                     /gene="ftsB"
                     /locus_tag="JN749_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HAY7367661.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(11579..11902)
                     /locus_tag="JN749_001182"
     CDS             complement(11579..11902)
                     /locus_tag="JN749_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HAY7367662.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="JN749_001183"
     CDS             complement(11952..12557)
                     /gene="cysC"
                     /locus_tag="JN749_001183"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HAY7367663.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="JN749_001184"
     CDS             complement(12557..13984)
                     /gene="cysN"
                     /locus_tag="JN749_001184"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HAY7367664.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="JN749_001185"
     CDS             complement(13986..14894)
                     /gene="cysD"
                     /locus_tag="JN749_001185"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HAY7367665.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15146..16183
                     /gene="iap"
                     /locus_tag="JN749_001186"
     CDS             15146..16183
                     /gene="iap"
                     /locus_tag="JN749_001186"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HAY7367666.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(16269..17420)
                     /locus_tag="JN749_001187"
     CDS             complement(16269..17420)
                     /locus_tag="JN749_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HAY7367667.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKNRRGEKEGSDDYHLLCWHLLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     EFKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPRIEIPTFWDDDEGVELIKQLSW
                     YISATVVLADWTGSSTRFFPRVAQAIDIEDYWQKALVQAQNALTVFPPQAETAPFTGI
                     NTLFPFIENTTPLQQKVLDLDINQPGPQLFILEDVTGAGKTEAALILAHRLMAAGNAQ
                     GLFFGLPTMATANAMYDRLVKTWLAFYSPEVFPAPAEINRRNTVRFSRK"
     gene            complement(17614..17766)
                     /locus_tag="JN749_001188"
     CDS             complement(17614..17766)
                     /locus_tag="JN749_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HAY7367668.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="JN749_001189"
     CDS             complement(18030..18764)
                     /gene="cysH"
                     /locus_tag="JN749_001189"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7367669.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMVEEETRFFGLKRECGLHEG"
     gene            complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="JN749_001190"
     CDS             complement(18838..20550)
                     /gene="cysI"
                     /locus_tag="JN749_001190"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HAY7367670.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="JN749_001191"
     CDS             complement(20550..22349)
                     /gene="cysJ"
                     /locus_tag="JN749_001191"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HAY7367671.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            22665..23030
                     /gene="queD"
                     /locus_tag="JN749_001192"
     CDS             22665..23030
                     /gene="queD"
                     /locus_tag="JN749_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HAY7367672.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            23108..24379
                     /locus_tag="JN749_001193"
     CDS             23108..24379
                     /locus_tag="JN749_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7367673.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            24370..24630
                     /locus_tag="JN749_001194"
     CDS             24370..24630
                     /locus_tag="JN749_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7367674.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            24647..25222
                     /locus_tag="JN749_001195"
     CDS             24647..25222
                     /locus_tag="JN749_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HAY7367675.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(25370..26263)
                     /locus_tag="JN749_001196"
     CDS             complement(25370..26263)
                     /locus_tag="JN749_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HAY7367676.1"
                     /translation="MAGLFTPKDAAMNIAIVTINQENAAIASWLAAQDFSGCTLAHWQ
                     IEPQPVVAEQVLDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLD
                     IPTVSMRKSHWGNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPD
                     WLVSTEDLKNVSRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGG
                     VDAEKVIGISGHLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADID
                     VVDDWKVVLEALVTNIHAEYQ"
     gene            complement(26227..27006)
                     /locus_tag="JN749_001197"
     CDS             complement(26227..27006)
                     /locus_tag="JN749_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HAY7367677.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTLVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(26984..28393)
                     /locus_tag="JN749_001198"
     CDS             complement(26984..28393)
                     /locus_tag="JN749_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367678.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMSGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SIFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(28415..29869)
                     /locus_tag="JN749_001199"
                     /pseudo
     CDS             complement(28415..29869)
                     /locus_tag="JN749_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(29939..30725)
                     /locus_tag="JN749_001200"
                     /pseudo
     CDS             complement(29939..30725)
                     /locus_tag="JN749_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            31044..32321
                     /locus_tag="JN749_001201"
     CDS             31044..32321
                     /locus_tag="JN749_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367679.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            32348..33818
                     /locus_tag="JN749_001202"
                     /pseudo
     CDS             32348..33818
                     /locus_tag="JN749_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         8277 a         9068 c         8427 g         8503 t
ORIGIN      
        1 atcatcaata tcagtcatag tatttcctcg gttatttcaa atcagctttg tttcctttat
       61 ttaacagtcc gttcccgcgg actgtttttt tattcaggcc agcggatcat cttcttcctg
      121 catggcaaaa agtttttgca tccccagccg gaaatcgcgt tcaaattctt cgtcatgctc
      181 ttcttcatat tccggttgct gaactgccac cacattacca accgggcgat aaacattact
      241 cagccagggt tcagcctgtt tgtgttccag aatctgaata ccttcatctt cagcatcacg
      301 aattttttcc tcagcgcgtt tgcgcattcc tttaatacgc tgctgttgtt tgtgatattc
      361 agcactgaca gcaaaggctg cgcgtttatt gccgttccat atcgcctcac agataaagct
      421 gccgtccttg cgacgtaccg taattcgctc ggcgtcatga atgtcataac tgataagcac
      481 tttgcgaccg tgttcgtcac gcaactccgg cgcgtagtaa atattgttca gccactgaat
      541 ttcgcagcgt cttaccggtc gttccaccat tgggcggaac atgtcgcgca gctcaagatc
      601 actcagccac tcaatttcgg tgtcttcttc tgccaggcgt ttttttctga actctgtcgg
      661 gctgtaatgc cgtccgttcg gtttcatcgg cagctcagaa tgcgggcggt tgttgtacca
      721 ttccacgcct tcacgaatag cctcaataag ctccgcccac gatggcagct cgcgcattgc
      781 cgctttctgg cggtcgttca gaggcttgtc ctgttgcagg gcgttaaacg ctgaacgcag
      841 atcacggtta agtacacgca acgattcccg gtcagcaccc ttgccaaaat aggtgcgata
      901 cctcctggct atacgcatcg gcagtgtccg gttcaggcgt tcaataatgc cgcgtccctg
      961 tggattaccg gcaataccgg tagggtgttt aatccccagg cgtggcagaa tacccacgac
     1021 ctctttgtcc agcatgtcag ccgtttcacc ggaaccgtta tccgagtaat acaggaacgg
     1081 ttttccgtgg ttgcgaatgc cgtgctgtat cgcaccggca acggcgaaca cgttttcggc
     1141 aaggtcaaga ctccagccaa caacaaaccg tgttccacca tcgatcacaa acgtgacttc
     1201 cggtgagaat ggacgcccgt ggatcggatg cgcacatttc agcttcatac cgtgaccgtc
     1261 accaatccag acataattca ccggcatagc tgtccagtcg cggcgggtga atccttcaag
     1321 ctgacggtat tcactgccgg taacgcgccc tttttgtttc accacttccg gcagtttttt
     1381 catggcatac cggacaacat cataggaagg catcacctcc agcatgtacg gttcgtccgc
     1441 atggcgacgc tgccattctg cgacaaaatc ctcgtaggct tctgacatgg gtctgccgtt
     1501 cacctggcga tactgcgcca gaaattcagg cagccagctg atttcttccg gttttatttc
     1561 gtcgcgtttt cccggtgcca gtaacagcag ccgttcggcg gcgttctgcg actggttgta
     1621 tttcagaacc cactggtaaa gtggatcgcg gctgatagta cgattagcgc cacgttttgc
     1681 attggcgtta actgcggcct gttgcaggcg ctccggcagc tcacctttac gggctttttc
     1741 aacaagaaag ttaatggctt tcagtcgccc gaggttaccg gcgctttcca gcttcagcac
     1801 ttccaccacc agcgcagctc ttgcttcaga tacccagcgc tggttttcgg tcagcgcatt
     1861 caggcgttct tccatcagtt gtggagaacc gcgatatgct tcgattgcct ggacagcgtc
     1921 aggattgcgt gtttttctgg taacagcttt cacttcagaa acattgttct ctgtagccac
     1981 cagctgttcc acatagcgtt cacgcagtgc ctttcgtgtt atttcaggca gacaatcaat
     2041 gtggtactca atggcttttg tgccttcccg acggcgacta acgtcaccaa gatcacgaga
     2101 aaaacgctgt aacgcctgtc ggatgccttt tgtggtggct ggcattcctg gtacgccgag
     2161 caggtcattt acggtaacgt acgactgcat atcacgacgc cttcctgata aatccatcgt
     2221 tacgataccg gctgggccag atgatttctg gcggtacacc tatagcgtca gaaattacgc
     2281 gttcgtattt tttgtttggt gtgcgaacta ccgctttcag tgaatcctga gcataaccag
     2341 catggagtga aagctcccgg aaggaagacc acccgttatc tttgagcgcc agctgaatct
     2401 tcagcgaagg ccaatcgcgg tcactatctt ctctttgtct catctttcct ttaacctaca
     2461 aaataacccg cgcggatatc cgtatggata aagattaaga taccaaagat tccctataaa
     2521 gggttctttg gtgcttttat ggtttatttt ttaatttcat tatttatcaa tgaattagat
     2581 agaaaggaac caagaatgag aaaagcaccc caaggttctt ttcctgccga tggaaaagaa
     2641 ccgattatcg aaagaatctt taggttagta gaacgatacc cttcaagaaa tgcggctgct
     2701 cgggcatggg ggatcaatga aggcacttta aaaaattatt acaacaggcg ggattacgca
     2761 ccgataccac gcccgcatca gttaaaaaag atagcagaaa gcgaaaatgt taccttggaa
     2821 tggctcatga cgggagaggg tgatgttgaa ctaaaagatt ccaaagaacc caaaaaagag
     2881 aatgcggata atgttgatgc tcaaattcta atgttcttgt catttcttaa accacaagaa
     2941 aaacaacaag tagctgatgt tcttgggaga aaaggagcgg agcaattact cattcttctg
     3001 gatgagcaca tccaagagtt gcacgctcta gttggagttc gtagagccat agcgttaagc
     3061 cttggtaatt tgccagacga aaaggttaga gagatttacg cgttgtccga agctaagggc
     3121 gatcacttaa atctacctca gaaagcagcc agcgcataac ccagtgcaag tgcctagctg
     3181 tgctaaaatt acattggttt tggcgcttgc acccatcagc gctataataa acacctgaat
     3241 cgtttttgaa cggtctttaa aggctattgt aagaacctgt gagttatctt aaatgtttta
     3301 ttgatatcga accgtttaac atttttggac tcgctttatc ttgaaaacct tgagttccta
     3361 acttgttgat tccaatctat tcccgtcttt tttaatctct tcccggttat cttgaataaa
     3421 ttacctagtc atacgagata gaaaccgcca tcgccatcat tcaggcgaaa cagcggcaga
     3481 atatcgaaca ggttgatctc ttcgccatcg acggtgttct gcgcccaggc tggattggcg
     3541 cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc aatctgcttt
     3601 ttgaccgggg tgtttggtgg caggccaagg gaaatcgcgt ggttctgcca ggaaccgatg
     3661 gtattcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca cagcgcgggc
     3721 gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg ctcagcgttc
     3781 acttcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa aaagctgcgt
     3841 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
     3901 gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg gctaccacat
     3961 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
     4021 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
     4081 tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc
     4141 ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
     4201 taacgatcat gccgtcagta cgaaaagaac cggatgagat gatcgccgcc tgatccgccg
     4261 ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc gtttctagtt
     4321 caatgatggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatctcac
     4381 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
     4441 tcagtttcat tgcttattct cttaattgtt cgtatacgaa cattattatt aacatactcc
     4501 ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctaaaaaacc
     4561 gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttt
     4621 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
     4681 ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg tattgaacag
     4741 gtggcactga tagaagcagc agtcagcacc aaagcaacgc tagcagaaat gttggcaaga
     4801 atggaaaatc gtggcttagt cagacgtgaa catgacgtcg ctgacaagcg gcgacgcttt
     4861 gtctggctga ctgccgaagg agaaaagcta cttgctgcgg cgatacctat tggtgacagc
     4921 gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt tatgcagttg
     4981 gtgcgcaaga tgatgagcac atagaatgct gattgccggg taggacgctg acgtgtctta
     5041 tccaggcgac aaaaacacaa gcttcccaaa caaaagaaaa aggccagcct cgcttgagac
     5101 tggcctttct gacagatgct tacttactcg cggaacagcg cttcgatatt cagcccctgc
     5161 gtttgcagga tttcgcgcaa acggcgcagg ccttcaacct gaatctggcg aacacgttca
     5221 cgggtgaggc caatttcacg acctacatct tccagtgttg ccgcttcgta ccccagcaaa
     5281 ccgaatcgac gtgccagtac ttcacgctgt ttggcgttca gctcgaacag ccatttgacg
     5341 atgctctgct tcatatcgtc atcttgcgtg gtatcttccg gaccgttctc tttttcatcg
     5401 gccaggatgt ccagcaacgc tttttcggaa tcaccaccca gcggggtgtc taccgaggta
     5461 atgcgctcgt taagacgaag catacggctg acgtcatcaa ctggcttatc cagttgctct
     5521 gcgatctctt ccgcacttgg ttcatggtcc agcttatggg acaactcacg tgcggttcgc
     5581 aggtaaacgt tcagctcctt tacgatgtga atcggcaaac gaatagtacg ggtttggttc
     5641 ataatcgccc gttcaatcgt ctggcgaatc caccaggttg cgtatgttga gaagcggaaa
     5701 ccacgttccg ggtcaaactt ctctaccgcg cggatcagcc ccaagttgcc ctcttcgata
     5761 aggtccagca acgccagacc acgattgcca taacggcggg caatttttac caccagacgc
     5821 aagttactct cgatcatccg gcggcgagag gcgacatctc cacgcagtgc gcgacgcgca
     5881 aaataaactt cttcttcggc cgttaacagt ggtgaataac caatctcacc aaggtaaagc
     5941 tgagtcgcgt ccaacacacg ctgtgtggct ccctgcgata acagttcctc ttcggccaaa
     6001 tcgttatcac tgggttcctc ttctactaag gccttttcgt caaaaacctc aactccgttc
     6061 tcatcaaatt ccgcatcttc atttaaatca tgaactttca gcgtattctg actcataagg
     6121 tggctcctac ccgtgatccc ttgacggaac attcaagcaa aagcctggtt ccgccgattt
     6181 atcgctgcgg caaataacgc agcgggttta cggatttccc cttgtaacga atttcaaaat
     6241 gcaagcgtgt tgaactggtt ccggtgctac ccatggttgc tattttttgc cccgccttca
     6301 cttcttgttg ttcccggacc agcattgtgt cgttatgggc gtaggcactc aggtaatcat
     6361 cattatgttt gatgataatc agattaccgt agccgcgcag cgcgttacca gcataaacaa
     6421 cgcggccatc tgcggtcgcg ataattgcct gtcctttgct gcctgcgata tcaatcccct
     6481 tgttgccccc ctcagaagcg ccaaaggttt cgatcacttt gccctcagtc ggccagcgcc
     6541 aggtggagat aggcgtactg gttgatgtac tgctgacagt cggctcggtt gtgcttgctg
     6601 ttggtaccgt tacaggcgct gtgaccgtgg tcgcagttgg cttgttgttc ggcaacattt
     6661 tgttagcact ctgttcaccc gaagactcag aatacgtaat tgtcggttgc gacgcaacag
     6721 caacggtgga attttgtgca ggcttgatca caactccttg ctctgctgcg tcggcctggg
     6781 taatagcatt tccgccagtg attggcgtac cggaagcatt acccacctgc aaggtctgac
     6841 caacgttcag cgcgtatggt gcctgaatat tgttgcgctg agcaaggtca cggaaatcgt
     6901 tgccagtaat ccaggcgata tagaaaagtg tgtcgccttt tttcacggta taggtactgc
     6961 cgctataact gcctttcgga atgttcccat actgacggtt atagacgatg cgtccgtttt
     7021 ccatctgtac cggctgctga gctactggct gcactggctg gatttgcggt tgttgagtag
     7081 cctgaatttg tggctgctgt accggctgaa tttgcggttg ctgcgctgta gacgtcgtcc
     7141 ctattttcgg cggcggcgta atcaacatac cagaattagt atttgcaggc gcattgccat
     7201 taacggagct gaccggtgcc ggtggatttg aagtgtcaga acagcctgcc agccatagcg
     7261 aaaccagtga caaagccgca atgcggcgaa cggtgaattt tgggcttccc gcgctcattt
     7321 atcccccagg aaaaattggt taataaccag tgacataatt accgtgcaag gcaccctact
     7381 gaacactgga aaagatgttc acgatacgct gacctacggc aaaataacca ggaaaaatcc
     7441 aggtatttcc tcacgtttta agccagctca cccttcacta aagggacaaa gcgcacggcc
     7501 tccacggtat cgataataaa ttcgcctccc cgacgacgca cccgtttcaa atactggtgc
     7561 tcctccccta cgggtaagac gagaatcccg ccttcgtcca gctgcgtcat tagcgcagtt
     7621 ggaatttccg gcggtgccgc cgtaacaatg atagcgtcaa acggcgcacg tgcctgccaa
     7681 ccttgccatc catcaccatg acgggttgaa acattatgta aatcaagatt tttcaggcgg
     7741 cgacgtgcct gccactgcaa gcctttaatc cgttcaaccg agcaaacatg ctggacaaga
     7801 tgcgccagga ttgccgtttg atatcccgaa ccagtgccaa tttccagcac ccgcgactgc
     7861 ggcgtcagct cgagtaattc ggtcattcgc gccaccatat acggctgcga aattgtctgc
     7921 ccctgaccta tcggcaaggc gatattgtcc caggcttttt gttcaaacgc ttcatcaacg
     7981 aatttttcac gcggcacggc ggcaagtgca ttcagcacct gctcatcctg aataccttgc
     8041 gcacgtaatt gatccagaag tgcttgtacg cgtctgctta ccattgcgtg ccaactccca
     8101 cgctgtttaa ccagtctgaa accacatctt gcgcgctatg cgcagttaaa tccacatgca
     8161 gcggcgtgat ggagacatag ccctcatcta ccgcagcaaa atcggtcccc ggaccggcat
     8221 cacatttacc gcccggcggg ccaatccagt acagcgtatt gccgcgcgga tcttgctgcg
     8281 ggatcacctg atctgccgga tgtcgtgtac cgcagcgcgt cacgcgaata cctttgattt
     8341 gatccaaggg taaatccgga acgttaatat taagaatacg tccggtgcgc agcggctctt
     8401 tacacagtgc gcgcaaaatt gaacaggtta ccgccgcggc agtgtcgtaa tgtttatgcc
     8461 cgtcaagcga gacggccagc gccggaaaac ctaaatgacg gccttccatc gcggcggcta
     8521 ccgtaccgga ataaataaca tcatccccca gattcggccc ggcgttaatt ccggacacaa
     8581 caatgtccgg gcgcggacgc atcagagcat tcacgccaag atagacacaa tcggtcgggg
     8641 ttcccatttg cacagcaata tcaccatttt caaaggtaaa cgtgcgcagg gaggattcca
     8701 gtgtcagaga atttgaagcg ctgctgcggt tacgatcggg ggcgaccacc tgaacgtcag
     8761 caaactcacg caaggctttc gccagcgttt gtataccggg tgcatgtacc ccgtcatcat
     8821 tactcagcaa tatgcgcata atcacctgtt gtgttgataa gttccctgac aacgctggtt
     8881 gcaaaactac ccgccggaag ccagaaacgg atctctacgg tgacgtcatc ccaccagttc
     8941 cagcttaatt gttgcggata cagcagcatc gctctgcgcg cggcttcaac tttttcgcgc
     9001 accagtaaag cttgtaattc agtttctgcg gcgacagctg cttgttcgaa tgccagcgct
     9061 tcacgctgag ttccccattc gccactgccc ggcaatgcgg cggttatcat cagctcttta
     9121 tcgttgacgc gacgctgtaa ttccgccagt tcttcggtgg ttgctacaaa ccagctacca
     9181 cgtccggcta attgtagcgc atcgccgtca acaacttgat taacgtctgc ttttttgagg
     9241 cgctcagcaa caatctgatt aaacaacgca ctgcgggctg ccgacaacca aaaactccgt
     9301 ttattgcgat cgcgcaccgg agtattggtt tgcgcccagc gcagcgcgcc ctgcaagttg
     9361 ctaccgccaa tcccaaaacg ttgggcaccg aagtagttcg gtacaccttt tgcgcaaata
     9421 tcgatcagac gttgttcaac gtcatcgcga ttgctcactt cgcgcagaac cagggtaaag
     9481 gcgttacctt tcagcgcccc taaacgcagc ttgcgcttgt gccgcgcata ctccagcacc
     9541 tggcagcctt ccagttgaaa ggcgctcaga tcgggcattt ccttgcccgg cacgcgagca
     9601 cataaccact gttccgttac agcatgtttg tctttttgcc cagcgaagct gacttcacgg
     9661 gaatgaattt tcaggaattt cgccagtgca tccgccacaa aacgggtatt gcagccgttt
     9721 ttgaggattc taaccagaat atgctcacct tcaccatcag gctcaaagcc caaatcttcc
     9781 accaccacaa agtcttccgg attggctttc agcagcccgg tgccttgcgg tttaccgtgg
     9841 aggtaagtga gattatcaaa ctcaatcatt ttgttgcctt aatgagtagc gccaccgctt
     9901 cacaggcaat cccttcccca cgtccggtaa atccaagttt ttccgtagta gtggctttca
     9961 cgttaacatc atccatatgg cagccgagat cttcggcaat aaacacgcgc atttgtggaa
    10021 tgtgcggcaa catcttcggt gcctgagcga tgatagtgac atcgacgttg ccaagggtat
    10081 aacccttcgc ctgaatacga cgccaggctt cgcgtagcag ctcgcggctg tcggcacctt
    10141 taaatgccgg atcggtatcc gggaacagtt tgccgatatc ccccagcgcc gccgcgccaa
    10201 gcaatgcatc ggtcaacgca tgaagcgcca cgtcgccatc agaatgcgcc agcaatcctt
    10261 tttcgtaagg aatgcgtacg ccaccaatga taattgggcc ttcaccgcca aaggcgtgta
    10321 cgtcaaaacc gtgtccaatt cgcattatgt attctcctga tggatggttc gggtgaggta
    10381 aaactcggcc agtgccaaat cttccgggcg cgtgacttta atgttatccg cgcggccttc
    10441 gaccaactgc ggatgaaatc cgcaatattc cagcgccgag gcttcgtcgg taatggtagc
    10501 accttcattt agagcgcgcg tcagacagtc atgtaacagc tcacgaggga aaaattgcgg
    10561 cgtcagcgcg tgccataagc cgttgcgatc aacggtatga gcaatggcat ttttgcccgg
    10621 ttcggcacgt ttcatagtat cgcgcactgg tgcggcgagg atccctcccg tgcggctggt
    10681 ttcgctcaac gccaacaatc gcgcgaggtc atcctgatgc aaacaaggac gagcggcgtc
    10741 atgcaccaat acccactgca catcgccagc ggctttcaga cctgccagca cggaatcggc
    10801 acgctcatca ccgccatcta caacggtgat ttgcggatga ttcgccagag gaagttgtgc
    10861 aaaacggcta tcgccaggac ttatggcaat gacgacacgt gtcacccggg gatgcgccag
    10921 cagcgcatgc accgagtgtt caagaatggt ttgattaccg attgagagat attgcttagg
    10981 acattccgtt tgcattcgac ggccaaatcc ggccgccgga accacggcgc aaacatccaa
    11041 atgagtggtt gccatgttaa ttcccgggct gatttatcga ttgttttgcc ccgcagactg
    11101 tgcgcgcttc gacgcgtcag gcaccagacg ataaaaagtt tcgcccggcc tggtcatgct
    11161 gagttcatta cgcgcacgct cttcgagcgc ctcctggccg ccattgagat cgtcaatttc
    11221 ggcaaaaagt tgatcgtttc gcgctttaag tttcgcgttt gtagcttgca gtgccgccac
    11281 atcatcattg acgcgggtat agtcatgtat accgttctta ccgaaccaca gcgaatactg
    11341 tagccagacc agaatagcca gcaacagcag cgttagttta cccatcctgc cccctgaaaa
    11401 actgcatcat catcccatgc atccgaagac gactctacat cctctgttgg ggataccgcg
    11461 acaacgcggg caaatgtacc acatttgtcc attgttacgt atacccaggg cgtgcagaac
    11521 ataatctcat tattagttac ggtttgaatt atgaacagag gagacaagaa agtacaaatt
    11581 agcccagtag ccacataaac agtgcgccaa acataatgcc tactgtcacc agggtgaaaa
    11641 caatactgta gcgtagcttt ccgtccatca atgaatgcag cgcaatcccc accactaccg
    11701 cgacaggcat cagtgccaga aagaaaggcc aggtgtagat aaagaagaac agcgtgttag
    11761 agccataaat caacatcggc atcgccagcg caaataacca ggagataaaa ccgaccacgg
    11821 caccaggcag tgaccatgta gtttcttcat cctcagtaag gctgtcgtta tttgttagtg
    11881 taatgttatg gctattacgc atatttgatc ctgttacttt gacgaaccgg gcattgaaac
    11941 ccggtggtgt ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt
    12001 accaaatttg ttactaattg ttcaccattg agatgaattt ctgccgattc aggctcttcg
    12061 taaacggaat ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataag
    12121 ccttttggat cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcaataaag
    12181 cgcccttctc ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat
    12241 gcggtcagca ccaccagtcc ggctgcaacc atcaaattcg ccacttcacc gacgcgacgg
    12301 atattctctt tacgatcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaaca
    12361 ttgtcgccat ccagcagata cgtactgacg ccgagtttat gtaacgcctc ctccagcgcc
    12421 ccggcgaccg ttgatttacc ggacccggag aggccggtaa accacagcac tacaccacga
    12481 tgaccgtggt gtagttcgcg ttgttgcaca gtgaccggat gactatgcca gacgacgttt
    12541 tcgtcatgca gcgccattat ttatccccca gcaaatcgcg cgcgccccag tgcggaaagt
    12601 ggcgacgaac cagggcattc aattccagtt cgaatgcact gaattcagat gacgcagctg
    12661 ttgcctggct aactggctcg tgcaccatac cggcacccac ggtcacattg ctcaggcgat
    12721 cgataaaaat cagcccaccg gtaaccggat tttgctgata gcgatctaac accagtggct
    12781 cgtcaaaagt gagatccacc aggccgatgc cgttcagcgg caggttttca acttcgcgtt
    12841 gggtaaggtt attaatatca acctgataac gaatgccatc aacacgagca cgcgtcttct
    12901 taccggcaat tttgatgtca taactctggc ccggggaaag cggctgttcc gccatccata
    12961 ccacatccac cgacgcactc tgcaccgccg gtaacgcttc gtctgccgcc agcagcagat
    13021 caccacggct gatatcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaaga
    13081 cttcttcgcg atcaccatca aaagtcacga tccgcgcgac gtttgattcc acaccagagg
    13141 gcagcacttt tacacgttgc ccgacttcca cgcgaccgga tgccagcatt ccggcgtagc
    13201 cacgaaaatc gagatttggg cggttaacgt actgcaccgg gaagcgcatt ggctgggcat
    13261 ccaccactcg ctggatctcc acggtttcca gcacttcgag cagtgtcaga ccgctgtacc
    13321 acggcatact ttcactttgc gaagccacgt tgtcaccttc cagtgcggag agcggcacaa
    13381 agcggatatc cagattaccc ggcagctgcc cggcaaaggt cagataatct tcacgaatac
    13441 gggtgaacgt ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat
    13501 gtttgatccc caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt
    13561 tacgggcatc aatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc
    13621 gagtgtactg ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga
    13681 aatagcggta ggccacatca atagtgatgc cctgttcgcg ctcagcttgc aggccgtcca
    13741 ccagcagagc cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca
    13801 gcgatgagag ctgatcttcg tagatttggc gggtatcgtg cagcagacga ccaatcaggg
    13861 tacttttgcc gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt
    13921 gcgcaatcat ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtacggtgt
    13981 tcatcttaaa aatacccctg acgttttttc agctccatgg atcccgcctg gtcgcggtca
    14041 atcacgcgac cctgacgttc actggtggtg gaaaccagca tctcttcgat gatttccggc
    14101 agtgtttgtg catttgactc caccgcaccg gtcagcggcc agcagcccag cgtacggaaa
    14161 cgcaccatcc gttttttgat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc
    14221 atcatcaaca taccgtcgcg ttccagaacc ggacgttccg cagcgagata cagcggcaca
    14281 atgtcgatat tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc
    14341 gggaagacgc ggatgctttc gcctttgtta atttgcccgt tgtagttgtg ccacagctcc
    14401 gggcgctgat ttttcggatc ccagcgatgg aagcggtcac ggaaagagta gattcgctct
    14461 ttagcgcggg atttctcttc gtcacggcgc gcaccaccga aggcggcatc aaaaccgtat
    14521 ttgttcagcg cctgtttcag gccttcagtt ttcataatat cggtatgttt cgcgctgccg
    14581 tgcacgaatg gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagctcg
    14641 cagccgtagg ctttcgccgt acgatcgcgg aactcataca tctcgcggaa tttccagccg
    14701 gtatcgacat gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga
    14761 tgcagcatga cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct
    14821 gccgccacct cgcgaataat gtggatgctt tccgcctcca gttgccgcag gtgagtaagt
    14881 cgtttttgat ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc
    14941 gactataggg agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac
    15001 ggaataacga tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt
    15061 ttaggaagat ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa
    15121 tttgcatggt atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct
    15181 ggcgctcggc gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt
    15241 tgctaatacc caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc
    15301 agcagaaatg ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag
    15361 tgatattcgg acatttaata gtcggtatat ttataccgcc cgcgataatc gcaagaactg
    15421 gcataacgtg acaggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat
    15481 catcattatg gcgcatctgg atacttacgc cccgctgagc gatgctgacg cagatgccaa
    15541 tctcggcgga ctgacgttac aaggaataga tgataacgcc gcaggtttag gtgtcatgct
    15601 ggaactggca gaacgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac
    15661 cagcggcgaa gaggaaggaa aattaggcgc tgagaattta ctcaagcgga tgagtgacac
    15721 cgaaaagaaa aatacgctgc tggtgattaa tctcgataac ttaattgttg gcgataaatt
    15781 gtatttcaac agcggtgtaa aaacccctga agcagtaagg aaattaacgc gcgacagggc
    15841 gctggcaatt gcacgcagtc acggaatagc cgcaacgacc aatccgggtt tgaataaaaa
    15901 ttatccgaaa ggcactgggt gttgtaatga cgcagaaata ttcgacaaag cgggcattgc
    15961 tgtactttcg gtggaagcga ctaactggaa tcttggaaat aaggatggtt atcagcaacg
    16021 cgcaaaaaca cctgccttcc cggcgggaaa tagctggcat gacgtaagac tggataatca
    16081 acaacatatt gataaggcac ttccaggaag aatagaacgt cgctgccgtg acgttatgcg
    16141 gataatgcta ccgttggtga aggagctggc gaaggcgtct tgatgggttt gaaaatggga
    16201 gctgggagtt ctaccgcagg ggcggggaat tctaagtgat acccatcatc gcatccagtg
    16261 cgtccggttt acttccggga aaaccgcacc gtattgcgcc ggtttatctc cgctggagcg
    16321 gggaacacct ctggcgaata gaaagcaagc caggttttga ccagtcgatc gtacatggca
    16381 ttggccgttg ccattgttgg caggccaaaa aacagcccct gagcgttacc cgcagccatc
    16441 aacctgtgcg ccaggataag cgccgcttct gttttacctg cgccggtcac atcttccaga
    16501 ataaacaact gtggtcctgg ctgattgata tccagatcca gcaccttttg ctgtaacggt
    16561 gtcgtattct caataaaagg aaacagcgta ttaattccgg tgaaaggcgc ggtttctgct
    16621 tgtggcggaa agacggttaa tgcgttttga gcctgaacta aagccttttg ccagtaatct
    16681 tcaatatcta ttgcctgtgc gacgcgtgga aaaaatcgcg ttgacgaccc tgtccagtct
    16741 gcaagtacga cggttgcaga gatataccag gaaagttgtt ttataagttc aacgccctcg
    16801 tcatcatccc agaatgtggg gatctctatt cgcggaaaca gtggcttgat ttcaaggaga
    16861 aaatctcgtg ctgcagcttt gtcttcaggc agaaaattat ccaattcatc catacgatca
    16921 gggggccgac catgatgccc tgtcgtcacg ggcatccaca tttctatcac tcgcctaagt
    16981 ttgcgcggag agagcgaaga tgaaggaagt aactcctgac attcacttaa ataatgattc
    17041 cacaaccagt agcccagcgt tgaatgagag attttttcgt aattttttct ggcaccttcc
    17101 tgaatcttga attcaggggg caggtaaagt tgttgaaaag agcgggcaaa ttttccaata
    17161 tcgtgccagc acagcaacca ggcgaaaaat tgagctgcct gttccttgtc agaaatccct
    17221 aattgacgaa aataatcagc cagcccgaag caatttcttt taaccattaa atagcccatt
    17281 gcggccacat ccagcaaatg ccagcaaaga aggtgatagt cgtcgctacc ctctttctcg
    17341 ccacgtcggt tttttcccca gaaatcaaag aaagtcacaa tatttttatc cttcagtaaa
    17401 cttaaaggat atttacgcat attttaaaaa ctatctgtga tatatatcag ccaataaaac
    17461 atttacatta tgaataaatt aatgattttc atttgaaatt cataataata aatagagact
    17521 atatattcac ataaaaaata taaattttaa aaaagggtgc aggagaccaa accatcacaa
    17581 ttcagtacgc ggggttttac ccccgcgtgg tggctacttc ttcgattcgt aagcgagaac
    17641 tgccttaaac tccataccac gttctctgat actcaactca cataacgagt cacctaccat
    17701 caacgtgaat cccagtactg tacaacacag tacgatgatt gccacaaggg catattttgt
    17761 cagcatgtct tgcctccctg caaagaagag actaacatcc gaattgctac gttcgaacgc
    17821 gagcctcagg ttgatagaaa tatcgcctgg ggcttttgtc catctggaac ctcgcgaatg
    17881 cttaacgcca gacagcctca agcacccaac gccattctat acctgttaat tttccctgcg
    17941 atgttcacct ttaaatgccg gaatgctgct cgcaaaataa aaacgccccc atcatttctg
    18001 acagaggcgt ttaatttatc cggcaatatt tacccttcgt gtaatccaca ttcccttttt
    18061 aagccaaaga aacgcgtttc ttcttccacc atgccgggtt cccatttacg ggttgtatgg
    18121 gtatcgccca ccgataaata tccttcgtcc cataatgggt gatatttcag gccatgtttt
    18181 tgcaggtact gataaatagt tcggttatcc cagtcgataa tcggcagcac tttaaatacg
    18241 ccacgctgaa ttgccagcac cggtaaattg gcacggctgc cggattgttc acgccgcagg
    18301 ccagcaaacc acgtttgcgc attcagttct ttcagcgccc ggttcatcgg ttcgactttg
    18361 ttgatgtcat tgtacttttc aatgccttca acgccctgtt cccacagttt gccgtagcgt
    18421 gcttcctgcc aggcggcgct ttcggtagcg cggtacactt tcaggttgag cttgagtttg
    18481 tccgttaact cgtcaataaa gcggtaggtt tccgggaaca gatagccggt gtcggtgagg
    18541 atcaccggaa tatccgggtg aatctgattc accagatgca ggctcaccgc cgcctgaatg
    18601 ccaaagctgg aagaaagtac atattcaccg ggcagattat ccagcgccca ggcaacgcgg
    18661 ccttcagcgt ccagtttttc cagttcggcg ttagtttctg ccagcgccag aatgcgatct
    18721 accttcggca gttcgttcag ggcgtttaga tcgagtttgg acatagattc ctcactgttt
    18781 gccttgcctg atgcgacgct cacgcgtctt atcaggccta caagaccggg ctgatggtta
    18841 atcccacaaa tcgcgcgccg gatcgagcac cgggcgaatg atgcccgcac gcaccgtaaa
    18901 gtcgccgaag ccttcacccg cttcgcgctc tttcgcccag cgccctatca gttcatcaag
    18961 cgacgccagg atttccggct cggtgatgtt ttctttatac atccgtggga tacgtgtccc
    19021 aatgcggttg ccgccaagat gcaggttgta gcgacccggt gctttaccca ccaggcccac
    19081 ttccgccagc atcgcgcgac cacaaccgtt cgggcagcct gttacacgca tcacgatatg
    19141 ctcatcgctg acaccatgtt tcgccattaa attatcgatg ttgtcgataa aagacggcag
    19201 gaaacgctcc gcttccgcca tcgccagcgg gcaagtcggg aatgacacgc aggccatcga
    19261 gttttcacgc tgcggcgtga cggcattcat taacccgctc tctttggcaa ttttctcgat
    19321 cttcgctttc tcgctttccg gcacaccggc gatgatcaga ttctggttcg ccgtaatgcg
    19381 gaaatcacct ttgtggatct tcgcgatctc cagcaggcca gttttcagcg gacgccccgg
    19441 ataatcaagg atgcgaccat tttcgataaa cagcgtcagg tgccagttat catcaatgcc
    19501 cttaacccag ccaatacgat cgccgcgtcc tgtaaattca tacgggcgga tcggttcaaa
    19561 tttgatcccc gcgcgacgct ccacttccgc tttaaacgtc tcaaccccca cgcgctccag
    19621 tgtgtatttt gttttggcat ttttacgatc ggttcggtta ccccagtcac gctgagttgt
    19681 cacgacggct tccgccaccg ctagcgtatg ctctagcggc agatagccaa actcactcgc
    19741 cgtgcgggcg taggttttct tgttaccgtg ttcgatagaa agcccaccgc ccaccagcag
    19801 gttgaagccc accagcttgc cgttttccgc gatcgccacg aagttcatgt cgttggcgtg
    19861 cagatcaata tcgttctgcg gcgggatcac taccgtggtt ttgaacttac gcggcaggta
    19921 ggtctgacca aggatcggtt cttcatcggt ggtggcgact ttttcctgat cgagccagat
    19981 ctccgcatac gcgcgggtac gcggcagcag atgttcagaa atcttcttcg cccactcgta
    20041 cgcttctgcg tgcagctgcg actcgtaagg gttcgaggtg cagagtacgt tacggttcat
    20101 gtcgttagca gtcgccagcg catcaagacc gaccgagtgc agcatctggt gcaccggttt
    20161 gacgttcttt ttcagaatgc cgtggaactg aaacgtctgg cggttggtca ggcgaatgct
    20221 gccatagatg gtgttttcac cggcaaattt atcgatcgcc tgccactgtt tggtggtgat
    20281 aactccaccc ggcagacggc agcgcaacag catcgcgtgg cgcggctcca gcttctgttc
    20341 agcacgttcg gcgcggatgt cgcggtcatc ctgctgatac atgccgtgga agcggatcag
    20401 caggaagtta tcgcccttaa agccgccggt cagaccgtcg tttaaatctt ccgcaatggt
    20461 gccgcgcagg tagttgcttt caagcttcat gcgctcggcg tctgtcagtt ttccttcgac
    20521 cactaaaggc cctggatgtt tttcgctcat tagtagacat ctcgctgata acggcgctct
    20581 acgcgcagct cacttaaaaa ttcatccgcc gcttcggtgt ccatgccacc aaattcggca
    20641 atcacttcca gaagtgcctg ctcaacgtct ttcgccatgc gattagcgtc gccgcagaca
    20701 taaatgtggg caccgtcatt gatccagcgc cacagctccg cgccctgttc gcgcagttta
    20761 tcttgtacgt aaactttttc tttttggtcg cgcgaccagg cgagatcgat ccgcgtcagc
    20821 acaccctctt tgacgtagcg ctgccactcc acctgataca ggaagtcttc cgtaaaatgc
    20881 ggattgccaa agaacagcca gtttttacct ggcgcttcgt cagcggcgcg ttgctgcata
    20941 aaggcgcgga acggcgcaat accggtgcct gggccaatca taatcaccgg ggtttccagg
    21001 ttagcgggca ggcggaagtt atcgttatgt tcgataaata cgcggacttc gccctcttct
    21061 tccacgcggt cagcgaggaa gctggaggca ccaccggcac gggcgcggcc ttccacgtcg
    21121 taacgcacca caccaacggt gacgtgtact tcgttctcga cttccgcctg cgaggaggca
    21181 atggaataga gacgcggggt cagcgggcgc agcagattaa ttagcgcttc ggcatcaagc
    21241 tgtgccgggg agaaacgcac catgtcaaca atcagcgtcg tcgcggcgta atgctgtaac
    21301 ttcgctttat cgcccaccag cggcagcagt gtttcactgc gggtaagcgt ggcgtaattc
    21361 tcaacaatgt tggcggtgtt gacggtcagt tcgaagtgcc actgtagcgc ttcgttcaga
    21421 ggcaacgttt tgccctcgac agtgacaggt tcatcgcctg tcagccacag cagttcgaca
    21481 agttctttca ccagtgccgg atcgttctga taccagacgc ccagcgcgtc acccggctgg
    21541 taacgcaggc ccgagtcgcc taagtcaatt tcgatatggc gaacgtcttt ttcagagtta
    21601 cgcccagtaa ttttctggtt aacagagagg ctagccacca gcggcgcgtc tttgctgtac
    21661 gggctggtgt ggatttcatt taccgcgcca gtagcgacgg attgcgaagg tgccgcgaca
    21721 ggcgcacgcg atttaagcgc atcaaccacg cgggcgcgcc actcgctggc agcagcctgg
    21781 tattcaacat cagaatcgac acggtcgagc aggcgttcac cacccagttc cgccagcttg
    21841 ctgtcaaaat ctttcccgga ctggcagaaa aattcataag agctatcgcc gaggctaaac
    21901 acggcaaacg cggtgttttc cagctttggc gcttttttgg agaacaggaa cttatgcagc
    21961 gcgacggctt cttccggcgg ttccccttcc ccttgcgttg acgtcactac gatgagcagt
    22021 ttttcgctgg cgatttgttt gaatttatag tcgcccgcgt tcaccagctt aacgttcagt
    22081 tttgctgcta ataaatcatc gcgtaatgct tcagcaaccc ggcgcgcatt gccggtttgc
    22141 gaagcagaga taatcgttat acccggcatt tctgcggcgg gcgctggcgt cactgcaagc
    22201 gcaacaggct gctggttgag cacgccccag aaatagccag aaacccaggc aagctgggta
    22261 ggcgttaaat cggtcgtggc cgcctgaagg cgtgccagtt gctccgggtt caacggaagc
    22321 aacgcggaag gtgggacctg tgtcgtcatg cgtcgttatg ttccagtaag caaagctgtt
    22381 tctgcgccct gtcagcgccc ataaaacaga agagaaggta aggttaacgg ggtgaacggt
    22441 gtggattaaa gacgggatag cgataactaa taaccaaatc gactaacctg ttttagcaat
    22501 agtctttaac aacaaaatag attaaccaac ctaatgaaaa acaaatgaat ttagccaatc
    22561 attaagataa atcagcgatt ttgcgcaaca aagtcgtttt agataatgcg aaaaaacagc
    22621 ctttccggta ctctacggcg gttttatcgt cctgtagaga aattatgatg tccaccacgt
    22681 tatttaaaga tttcaccttc gaagccgctc accgcttacc acatgtcccg gaagggcata
    22741 aatgtggtcg cctgcacggg cattccttta tggtgcgact ggaaattacc ggggaagtcg
    22801 atccgcatac gggctggatt atcgatttcg ctgaactgaa agcggcgttt aagccaactt
    22861 atgagcgcct agatcaccat tatctcaatg atattccagg tctggaaaac ccaaccagcg
    22921 aagttttagc aaaatggatt tgggatcagg ttaaacccgt tgtgccgctg ttaagtgcgg
    22981 tgatggtaaa agaaacctgc accgcagggt gtatctatcg cggcgaatga taagagtgtg
    23041 tcggcggtca atttcccttt agtaacgcta tgttagggtg ttgtgttctg gatatctggg
    23101 gcatgacatg gaagacgact gcgacattat tattattggt gccggtattg caggcaccgc
    23161 ttgcgcgtta cgctgcgcgc gagcggattt atccgttttg ttactcgaac gcgctgaaat
    23221 ccccggcagc aaaaatcttt ccggcaggcg gttatatacc catgcactcg cggaactcct
    23281 cccgcaattt catctgaccg cgcctcttga acgacgcatc actcacgaaa gcctttccct
    23341 gttaacgccg gatggcgcaa cgacgttttc cagcttacag cccggcggtg aatcctggag
    23401 tgtattacgt gcacggttcg atccgtggct ggttgccgaa gccgaaaaag aaggtgtcga
    23461 atgcatcccc ggagcgacgg tggatgcact gtatgaagaa aacggcagag tctgtggcgt
    23521 tatttgtggt gacgatattc tccgcgcccg ttatgtggtg ctggcagaag gtgccaacag
    23581 cgtcctggct gaacgtcacg ggttagtgac tcgtcctgct ggcgaagcga tggcgttggg
    23641 gatcaaagaa gtgctgtcgc tggaaacatc cgctattgaa gaacgttttc atctggagaa
    23701 taacgaaggc gcagcgttgc tgttcagcgg cggaatctgt gatgacttac ccggcggcgc
    23761 atttctttat actaatcaac aaacgctctc gttagggatt gtttgcccgc tctcttccct
    23821 tacgcaaagt cgtgttccgg caagcgagct gctgactcgc tttaaagcgc atccggcagt
    23881 gcgcacgctt atcaaaaaca cggaatcact ggagtatggt gcgcatctgg tgccagaagg
    23941 tggcttgcac agtatgccgg tgcaatacgc cggtaacggc tggctgctgg tgggcgatgc
    24001 gttgcgcagt tgcgtcaata ccggaatttc cgtgcgcggc atggatatgg cgctgactgg
    24061 cgcacaggcg gcggcacaaa cgctaataag cgcctgccag caccgcgagc cgcaaaatct
    24121 gtttgcgctt tatcatcaca acgtagagcg cagcctgcta tgggatgttc tacaacgtta
    24181 tcagcatgtt ccggcgcttt tgcaacgccc tggctggtat cgggcgtggc ctgcgttaat
    24241 gcaggatatt tcccgcgatt tatgggatca gggtgataaa cctgttccac cgctgcgcca
    24301 gttattctgg cgtcatttac gtcgtcatgg cctgtggcat ctggcgggcg atgttatcag
    24361 gagtctgcga tgtctgtagc ccgtaatctc tggcgcgttg ctgatgcgcc gcacattgtt
    24421 ccggctgact ccgttgagcg tcagatggta caacggttga ttaacgcctg tccggtaggt
    24481 cttttttctc tcacacagga aggtggctta cgtattgact atcgcggctg cctggagtgt
    24541 ggcacctgcc gtttgctgtg cgacgaatcc acactacaac agtggcgcta tccgccttcc
    24601 ggattcggca tcacctaccg ctttggataa ataataagga taatttatgc ccctcttaca
    24661 cctgctccgc cagaatccgg tgattgctgc cgttaaagat aatgccagcc tgcaactggc
    24721 catcgattct gaatgccagt ttatttccgt gttgtacggc aatatctgca ccatcagtaa
    24781 tatcgttaaa aagattaaga acgccgggaa atatgctttt attcatgttg atttgctgga
    24841 aggcgcgtca aataaagaag tggtgattca gtttttgaaa ctggtgaccg aggcggatgg
    24901 aattatcagc accaaagcat cgatgctaaa agcggcaaga gcggaagttt ttttctgtat
    24961 tcatcgcctg tttattgttg attcgatttc gtttcacaac attgataagc aagttgcgca
    25021 atcgaatccg gattgtattg agatcctgcc aggctgcatg cccaaagtgc tgggctgggt
    25081 gacggaaaaa atccgccaac cgctgattgc tggcgggctg gtatgcgatg aagaagatgc
    25141 gcgtaatgcg attaacgcgg gtgtcgtggc gctttccacc acgaataccg gagtctggac
    25201 gttagcgaaa aaattgcttt gacgggataa tcgtaaccaa ttgaatttgt tttgatttgt
    25261 tggccgcacg ccacatccga cattcagcgc ctgatgcgac gcttaacgcg tcttatcagg
    25321 cctacaggtc cccagtccag aaccgtaggc aggatcatcc ggcagcacgt tattggtatt
    25381 ctgcgtgaat gtttgtcacc agtgcctcca gcaccacttt ccagtcatca accacgtcaa
    25441 tatctgcctg tgaaaatacc gctgcgctgg cgtcatgatt aatcgccacc ataaacttgc
    25501 tattgcgtac gcccgccatt aacgctgctg cgccggaagc gccaacaaca atgcacacct
    25561 caggtgccag cagatgtccg gaaataccaa tcactttttc agcatcaacg ccaccgttca
    25621 tcactcgcgc ccgactgtag cccacttcgg ttcccagctt ttctgccagc ataacgattt
    25681 cctggttatc tgcctctccg ccctgcccca ccaccagcac ccgtctggcc tcagctaatg
    25741 ggtcgcggct aacatttttc agatcctctg tgctaaccag ccagtcggga agcgcacctg
    25801 ggacaatgtt cagttgctgc attccagagg gcaacgtggc gtttttcgct gctcccgcct
    25861 gacgcgccag ggaaaggcat aacgggcgtt tttctgtttg cagcgtcgcc gttaacgcat
    25921 ttccccagtg ggatttgcgc atgcttacgg ttggaatatc taacgaagtc acctgacaga
    25981 tgcttgcacc atgtaaacgc caggccagcc gggtcgacag ttcatcgcca aacgttcccg
    26041 gcggaaagag aacaacgtcc gcaggcgttc gctgccactg ttccaccatc gcatccagca
    26101 cctgttccgc cactacgggc tgaggttcta tttgccagtg cgccagcgta caaccgctga
    26161 aatcctgcgc cgccagccag ctcgctattg cggcattctc ctgatttatt gtcacaattg
    26221 cgatattcat ggctgcatcc tttggcgtaa atagtcctgc catagctttt gagctttttc
    26281 tgctaccgtt tgcccatcaa tcagcgtcgc tccgcatcgt tgctctgctc ttgccagttg
    26341 cagacactgc atcgctggca tttcggcagc aacagtttta cggataattt ctgctttccc
    26401 cgcagccatt cgctggcgca ttcccggtac tggcaaggcc acttcaccgc actgacgcac
    26461 ggcaatcacc gcaggcaagc gaacccggca acagcgcaac ccatgttcag tacgctgttc
    26521 gagggtgata aacagcgcgt cgagagtgaa acgctccacc agggtgaagc agggccagcc
    26581 cagcatttcc gccagcaaaa atggcgtttg cccattctgc ccttcgctgc tttggcagcc
    26641 ggtaattatc agatccagcg gattctgacg ctgccattcg gcaatatggc gagcgacaaa
    26701 ttccggcgca aagcgcagat ctgccgccgt ctccagcaat acagcttcct caaacccaag
    26761 agccatgaga tagcgcagcc agtgcaacgc ccgttcatcc cccatgctca acgcggttaa
    26821 agacatcggc gtaccgtttt ttctctgcgc taacagcagc gcggcagcgg cctgttcatc
    26881 agcaccgagt aaacttcgca gtagcgaaac atccggtccg ctattaccct gagccgccgc
    26941 ctgccactct ttttccgcca gcattccggc atccggttcg gctttaaacg ctaacagaat
    27001 gttcattcgc acctcctaca tttcccgccg ccaccagcgg gagggcttta gtttctggtg
    27061 cccatagcca ggtcacaaca aaaccaacca acaacactgt cgccaggagc aataaggtga
    27121 cttgcattcc ccactgcgcc agcacccacg gcagcaggcc agtacttacc gccgcgccaa
    27181 gtcgactcat ggcggtggca aaaccgacgc ccagcgagcg aatgtcagta ggaaaacttt
    27241 ccgcaggcaa aatgcccacc agattactga ctgccgaaat ggtggtgctg aagagaacaa
    27301 aaagcagcag cgttaatgaa ctgccggaag gcaaacaggc cattactacc agcgttgccg
    27361 ccagcagcaa aaaacttccc agcaaaaatt tgcgatgtgc cagcaggtgc gtcagaacta
    27421 atcccagcag cgcgcccaca attaacaacg cattaagcat caggctggca gtcagcgcat
    27481 cttccagacc aatagtctgg gcgatagttg gcagccaggt ataaatcaca aaccatggga
    27541 ttacgaggca gacaaagaag atgctgttaa acgccgtgcg ccgccagtaa cgcgaagaga
    27601 acaaggtttt gatgtgttta tgggtcgccg ttaccacttc atcgcccagt aaaacatggg
    27661 gaccaaaata gcgatgcacg atagcgtgag cttctgcaaa acgcccctgg cacagtagcc
    27721 aacgtggcga ttctggcgtt ccccagcgta ataacgtaat caacaacgcg ggcagagctg
    27781 ccgatgccag caaccagcgc caggcctccg ggttttcgga aataaagtga tgtccggcaa
    27841 tacttgccaa cacatagcct acggtccaca ccacgctgaa tgcgcccagc aaaataccgc
    27901 gatggcggcg cggggaaaat tcagccagca aggtgtgacc tactgagtaa tcgcctccca
    27961 gaccaatgcc gatcaaaatg cgcagcccaa taagatgctc tggcgtggtg gcaaaaaatt
    28021 gcaagaacga agcgagcgta atcagcaaaa agctgaaggt gaagattttt tgccgaccaa
    28081 tatggtcgga gatccaccca agaaccaggc tgccaagaaa caaaccaagg agcgccgagc
    28141 cgccgctcat tcccgccata aacggcgtca gttgcatggc gggcgtaagt tgaataatgg
    28201 cgtaaccaat gacgccgaga acataaccgt cggtcaggtg tgcgccgaaa gtgagcgcag
    28261 caattcgaca gtgaaaacgg ttaagcggta aatcatccat tcgcaccggt gaagtgttca
    28321 tctttacccc ttcattgtcc ggtaataaga acggcttctc gcctgagaag ccgaaaaggc
    28381 aacgccccgt catcgcggaa cgctgcctga tacattattt ttcaatcgga tagatagtac
    28441 ccgtattcat aatgccgtta ggatcgaact gctttttcag cccttccagc aacgcccacg
    28501 cgctgccgtg ttccagcttg ctccagtgaa cgcgatgttt accaatgcca tggtggtgca
    28561 ccatcgaacc gccgaggcga atggtttctt cgcagatgat tttgttgagc ggattgtggt
    28621 acttgtcgat ttcctcttcc ggcttacagt caacaacgtt gtagtcatag acgaagtaca
    28681 tgttggtgcc gttctgatag ctatgagagg agtgaccgcc cagcatggtg atgtcgtcgg
    28741 cgtgcgggaa ctcagtacga atacggttaa taacgctttc gtagatttcg tggatgcagc
    28801 tccagcagcc ggacacttcg gtggtaaagc ccatgttgcc ggttttgagg atctgcacac
    28861 gttcggcagc cactttatcc ggtccccagt tcaggttgtt aaaccaggtt tcaatcagtt
    28921 tgctgtccac gcgctggcac tgcgggtagc gggcaacgat ttccgcaatc ccttcgcccg
    28981 tcgccttcgc aatgcgaggg ttaccttcag ccataaagat cagcacccat tttccgtcgg
    29041 caaaatgggt gaagtgttgg gtgccatctt cagcgtcata caaacgagcg atcgacggac
    29101 gatacccttc caccatgatt tcacgcagga tgttgaagcc ggttttcatg tcttccagga
    29161 tatagccgta gaagaggttg ttttccgggg tgtatttaaa gattttcact gttacttcag
    29221 tgatatagca caatgcacct tcgttgccga tgatgatgtg acgaatgtcc gggccagccg
    29281 cgcggcgtgg cacgttttta atgcgtgtga cggtgccatc tgccaacact gcctccagac
    29341 caacgaccat atcttcgatt gcgccgtaga gtgtggagaa ctgcccgata ctgcgggttg
    29401 ctaccaggcc gcccatctgc gccagcggct ttgactgcgg agaatgcccc gtggtgtaac
    29461 ctttttcacg caacgcgttt tccagcactt ccagcggaac accacattgc gccgtcgcct
    29521 gcatattctc aatatcaata ttaatgattt gattcatggc ggagccgtcg agcaccaccg
    29581 agttttctac aacagtttcc agcccacctt cggtggcgga agcaccggta cgcggcacac
    29641 cgttaatttt gtgcgcattc ataaaattca gcacacggga tacttactct gtggaaccga
    29701 gttttacgac cgctgccgga atcggcaaag tataaatgcc atgaatatcc ggaaatttac
    29761 gaaaacggtc aatactgttt ttctttaata ctgtttcatc ggtaattacg cgatctgcac
    29821 caacaatttc ctttagctgg tcgacaatcg ctgcgcgaga taaagacata gtaattcctt
    29881 cctgataata aaaatggaat aatcattaat aagagaaaac agaaaccacc gttatggatt
    29941 agcgcactaa ataaccgcca tcgaccacta ataaatgccc gttgacataa ttcgatgcct
    30001 gacttgcgag aaacacggct gcgcccatta aatcctgagt atcgccccaa cggtttgccg
    30061 gaatatgatc aagaacgcgc tgattggttt ctggattact gcgtgtcgcc agcataatat
    30121 gctgttgcat aatagccagg ggcgatacca tttacctgaa tattatattg acctagttca
    30181 tcacaataag ctttggtgaa cccggcaaga gcatgtttag tggcagaata tgcaggtgac
    30241 cattgtccac ctaagtaaga gaacaatgaa cagatattaa taattttgcc gcttttctgc
    30301 gggatcataa tttttgccgc ttcatagctt aactcgaatg cggcggtcag gttcacatca
    30361 atcatcggat cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg
    30421 gcattgttaa ccagaatatc gacagtaccg aagcgctcac agcaggcagc gataatcttc
    30481 tgcggtgcgc cttctgcggt aatatccacc tgcatgaagt ccacttcaac accctgtttt
    30541 tcaatcattt cctttgtttc gccgttatct ttgacgaagc taggaataaa aatatttgcg
    30601 ccagctttgg ccaacgccat tgcaaatgcc tgacctaaac cgctattccc gccggtaaca
    30661 attgctgttt tacctttcag ggagaaaaaa tccattgaga acgcattgag agattcgatt
    30721 gacatagtta gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag
    30781 agggaagtta cctttctcat tatctctggt aactggagat aatttttagc ataacggcat
    30841 aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag ttttaaaaag
    30901 cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct tattgcaatt
    30961 actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct cttttttttg
    31021 tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg ataaccctcg
    31081 cgataattag ttttagcggc ggcgttagtt ttgacctggc ttatttacgt tatatttatc
    31141 aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta ataatgagta
    31201 cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc gataaatttt
    31261 cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt ctgttaatgg
    31321 caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg ataacgacga
    31381 ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt gcttggcgat catagcgagc
    31441 aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg
    31501 tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc ctgaaaacag
    31561 tcattatcat ctacagtgtg gtttacatct tgttggggat tctgtgctgg ttttttgtta
    31621 gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc cagcttagcg
    31681 acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg atttttggtg
    31741 tcttcacaat ctacgccatt ctgagttact ccaccaacta tctgaccgaa atgtacggca
    31801 tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt
    31861 gcggcccact tggaggcatt atcaccacct acagtaaagt gaaatcccct acccgcgtga
    31921 ttcaaatcct ttccatcatc ggtttgctgg cgttgacagc cctgctcgtc acgaactcta
    31981 acccgcaatc ggtcgcgatg gggattggcc tgattttact gctgggattc acctgttacg
    32041 cctcacgcgg gctgtactgg gcctgccccg gcgaagcgag aacaccgtct tacattatgg
    32101 gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc gtttacccga
    32161 ttatcggcca ctggcaagac accctgcccg ctgcagaagc ctaccgcaat atgtggctga
    32221 tgggcatggc ggcgcttgcc atggtgatag tctttacctt tttgctgttc caaaaaattc
    32281 gtactgctga tagcgccccc gcaatggcta gcagcaagta agccaggaaa attacaggag
    32341 aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga gcacaaaagt
    32401 ggtgatatac gatctggaag gtaatgtggt ttgcgaaggg aaaggcttat tgcagccgat
    32461 gcacacgcca gatgccgata ctgcagaaca tcctgacgac gatttatggg catcattatg
    32521 ttttgccggt cacgatttga tgagtcagtt tgccgggaat aaagaagata ttgtcggtat
    32581 tggtctggga tccatccgtt gctgccgtgc gttattgaaa gccgatggca cggccgttga
    32641 ttagctggca ggatgcacgc gtaacgcgtc cctacgaaca cactaatcct gacgtggcat
    32701 atgtcacctc tttttcgggt tatctgacgc atcgcttaac cggcgagttt aaagacaata
    32761 tcgccaacta ttttggtcag tggccggtgg attataagag ctgggcatgg agcgaagatg
    32821 ctgcggtaat ggagaagttt aatatccccc gtcatatact gtttgatgtg caaatgccag
    32881 gcactgttct tgggcatatc acaccacaag ccgcgctggc gacacatttc ccagctgggc
    32941 tgccggttgt ttgtaccacc agtgataaac cggtagaagc tctgggggcc ggattactgg
    33001 atgatgaaac ggcggcgatt tcactcggta cttatatcgc gctgatgatg aacggcaaag
    33061 ccttgccgaa agatccggtg gcgtactggc cgattatgtc ttctattccg caaacattgc
    33121 tgtatgaagg ttacggtatt cgcaaaggta tgtggacggt gagctggctg cgcgacatgt
    33181 taggcgagtc gttaattcag gatgccaggg cgcaggatct ttcaccggaa gatttactca
    33241 acaagaaagc ctctagtgtg ccacctggct gtaatgggct gatgacggtg ctggattggc
    33301 tgaccaatcc gtgggaaccg tacaaacgcg ggattatgat cggctttgat tccagcatgg
    33361 attacgcatg gatatatcgt tcgatactgg aaagcgtggc gctgacgctg aagaacaatt
    33421 acgacaatat gtgtaatgaa atgaatcact ttgcgaagca tgtgatcatt actggcggcg
    33481 gttcgaacag cgatctgttt atgcagattt ttgccgacgt gttcaacctt ccggcacgac
    33541 gtaacgccat taacggttgt gcaagcctgg gggcagcgat taatacagcg gtaggtctgg
    33601 ggctataccc ggattacgca acggctgtcg acaaaatggt tcgcgtgaaa gatatcttta
    33661 tgcctgttga gagcaatgcc aaacgctacg acgcgatgaa taaaggtatt ttcaaagacc
    33721 taaccaaaca tactgatgtg atcctgaaaa aatcgtatga agtgatgcat ggggaattgg
    33781 ggaatgcgga ttcgatccag agctggtcga atgcgtaagt gaggaagacc gggcgggaaa
    33841 ctgcccggcc tgaatatacc tgaatggtta tccccgctga cgcggggaac tcgaataact
    33901 gaccaacgtc tggctctcta aatccggttt atccccgctg gcgcggggaa cactctaaac
    33961 ataacctatt attaattaat gattttttaa gccagtcaca atctaccaac tttacaggat
    34021 cacacaaaca acacatccat tatgttaaag agcacttaat ccattgatta aaaaggtaaa
    34081 tatttaaaat aactctatac aaactaaaat ctaccaaact tcgccgcaat aattttcact
    34141 ccagcgaaaa attaacgccg cagaatttgt agagaatgat aatgattagt ccgggatggg
    34201 caagatggat gagttttcag gggcgacaaa taatacaaat gagtggaatt ttttacaagt
    34261 tccacccatt tataa
//
LOCUS       DADLTF010000026        33393 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.026, whole genome shotgun sequence.
ACCESSION   DADLTF010000026 DADLTF010000000
VERSION     DADLTF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.026"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1272..2051
                     /gene="ipaJ"
                     /locus_tag="JN749_001203"
     CDS             1272..2051
                     /gene="ipaJ"
                     /locus_tag="JN749_001203"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HAY7367680.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2998..3927)
                     /locus_tag="JN749_001204"
     CDS             complement(2998..3927)
                     /locus_tag="JN749_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HAY7367681.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4144..4380)
                     /locus_tag="JN749_001205"
     CDS             complement(4144..4380)
                     /locus_tag="JN749_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAY7367682.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4384..6285)
                     /gene="ipaA"
                     /locus_tag="JN749_001206"
     CDS             complement(4384..6285)
                     /gene="ipaA"
                     /locus_tag="JN749_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HAY7367683.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTSPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6294..7292)
                     /gene="ipaD"
                     /locus_tag="JN749_001207"
     CDS             complement(6294..7292)
                     /gene="ipaD"
                     /locus_tag="JN749_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HAY7367684.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRN
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7343..8434)
                     /gene="ipaC"
                     /locus_tag="JN749_001208"
     CDS             complement(7343..8434)
                     /gene="ipaC"
                     /locus_tag="JN749_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HAY7367685.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(8454..10196)
                     /gene="ipaB"
                     /locus_tag="JN749_001209"
     CDS             complement(8454..10196)
                     /gene="ipaB"
                     /locus_tag="JN749_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HAY7367686.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINKTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10202..10669)
                     /gene="ipgC"
                     /locus_tag="JN749_001210"
     CDS             complement(10202..10669)
                     /gene="ipgC"
                     /locus_tag="JN749_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocator chaperone
                     IpgC"
                     /protein_id="HAY7367687.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10726..11352)
                     /gene="ipgB"
                     /locus_tag="JN749_001211"
     CDS             complement(10726..11352)
                     /gene="ipgB"
                     /locus_tag="JN749_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgB"
                     /protein_id="HAY7367688.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11368..11757)
                     /gene="ipgA"
                     /locus_tag="JN749_001212"
     CDS             complement(11368..11757)
                     /gene="ipgA"
                     /locus_tag="JN749_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HAY7367689.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11770..13254)
                     /gene="icsB"
                     /locus_tag="JN749_001213"
     CDS             complement(11770..13254)
                     /gene="icsB"
                     /locus_tag="JN749_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HAY7367690.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13568..15184
                     /locus_tag="JN749_001214"
     CDS             13568..15184
                     /locus_tag="JN749_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HAY7367691.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLPQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15194..15556
                     /gene="ipgE"
                     /locus_tag="JN749_001215"
     CDS             15194..15556
                     /gene="ipgE"
                     /locus_tag="JN749_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HAY7367692.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15556..16014
                     /gene="ipgF"
                     /locus_tag="JN749_001216"
     CDS             15556..16014
                     /gene="ipgF"
                     /locus_tag="JN749_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein IpgF"
                     /protein_id="HAY7367693.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16023..17138
                     /gene="mxiG"
                     /locus_tag="JN749_001217"
     CDS             16023..17138
                     /gene="mxiG"
                     /locus_tag="JN749_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HAY7367694.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKKIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17146..17397
                     /gene="mxiH"
                     /locus_tag="JN749_001218"
     CDS             17146..17397
                     /gene="mxiH"
                     /locus_tag="JN749_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex protein
                     MxiH"
                     /protein_id="HAY7367695.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17410..17703
                     /gene="mxiI"
                     /locus_tag="JN749_001219"
     CDS             17410..17703
                     /gene="mxiI"
                     /locus_tag="JN749_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HAY7367696.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17709..18434
                     /locus_tag="JN749_001220"
     CDS             17709..18434
                     /locus_tag="JN749_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HAY7367697.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18431..18958
                     /locus_tag="JN749_001221"
     CDS             18431..18958
                     /locus_tag="JN749_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HAY7367698.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18900..19595
                     /locus_tag="JN749_001222"
     CDS             18900..19595
                     /locus_tag="JN749_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MxiN"
                     /protein_id="HAY7367699.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKR
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19588..19995
                     /locus_tag="JN749_001223"
     CDS             19588..19995
                     /locus_tag="JN749_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa secretion machinery protein MxiL"
                     /protein_id="HAY7367700.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19979..20407
                     /gene="mxiM"
                     /locus_tag="JN749_001224"
     CDS             19979..20407
                     /gene="mxiM"
                     /locus_tag="JN749_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HAY7367701.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20537..21169
                     /gene="mxiE"
                     /locus_tag="JN749_001225"
     CDS             20537..21169
                     /gene="mxiE"
                     /locus_tag="JN749_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional activator MxiE"
                     /protein_id="HAY7367702.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21156..22856
                     /gene="mxiD"
                     /locus_tag="JN749_001226"
     CDS             21156..22856
                     /gene="mxiD"
                     /locus_tag="JN749_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HAY7367703.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSRLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22875..23942
                     /gene="mxiC"
                     /locus_tag="JN749_001227"
     CDS             22875..23942
                     /gene="mxiC"
                     /locus_tag="JN749_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HAY7367704.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23955..26015
                     /gene="mxiA"
                     /locus_tag="JN749_001228"
                     /gene_synonym="virH"
     CDS             23955..26015
                     /gene="mxiA"
                     /locus_tag="JN749_001228"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiA"
                     /protein_id="HAY7367705.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26028..26429
                     /gene="spaK"
                     /locus_tag="JN749_001229"
     CDS             26028..26429
                     /gene="spaK"
                     /locus_tag="JN749_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone SpaK"
                     /protein_id="HAY7367706.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26433..27725
                     /locus_tag="JN749_001230"
     CDS             26433..27725
                     /locus_tag="JN749_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HAY7367707.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27818..28174
                     /gene="spaM"
                     /locus_tag="JN749_001231"
     CDS             27818..28174
                     /gene="spaM"
                     /locus_tag="JN749_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface presentation of antigens protein SpaM"
                     /protein_id="HAY7367708.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIRQYKPKF"
     gene            28143..29021
                     /gene="spa32"
                     /locus_tag="JN749_001232"
     CDS             28143..29021
                     /gene="spa32"
                     /locus_tag="JN749_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HAY7367709.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIAANYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29015..29896
                     /gene="spaO"
                     /locus_tag="JN749_001233"
     CDS             29015..29896
                     /gene="spaO"
                     /locus_tag="JN749_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein SpaO"
                     /protein_id="HAY7367710.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29901..30551
                     /gene="spaP"
                     /locus_tag="JN749_001234"
     CDS             29901..30551
                     /gene="spaP"
                     /locus_tag="JN749_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaP"
                     /protein_id="HAY7367711.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30566..30826
                     /gene="spaQ"
                     /locus_tag="JN749_001235"
     CDS             30566..30826
                     /gene="spaQ"
                     /locus_tag="JN749_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HAY7367712.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30826..31596
                     /gene="spaR"
                     /locus_tag="JN749_001236"
     CDS             30826..31596
                     /gene="spaR"
                     /locus_tag="JN749_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaR"
                     /protein_id="HAY7367713.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31603..32631
                     /gene="spaS"
                     /locus_tag="JN749_001237"
     CDS             31603..32631
                     /gene="spaS"
                     /locus_tag="JN749_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein SpaS"
                     /protein_id="HAY7367714.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSVFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32644..32886
                     /locus_tag="JN749_001238"
     CDS             32644..32886
                     /locus_tag="JN749_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367715.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10432 a         4844 c         6676 g        11441 t
ORIGIN      
        1 tgacacttgt tgactacctg tgcggcatct ggtatcaagg tggcgtgaag ccggttcagg
       61 ttctcatgcg gaacgttgga acctatatcc ccccgatacc aaaggcgaaa tgtaagtgac
      121 aaaatgcaat gaacacgttc tccgttaact gaggagcatg gtgataaata aaggtaaatg
      181 cagaaggtgt agagtctcgc agatttctga catcatagag cctaacatta gatgcataac
      241 gctacagatt gtagaatctc tacaggtgaa atatacactg gttggcgttt atgtcgcaaa
      301 acctgtcatt caggttcatc tcaccaataa ggatatgagt gaagtggagg ataagtgagt
      361 ctgctgtcag agttttctgg tgtatgtcag taactgcgaa ccatgtctgc ttgatatggg
      421 agcttatgga ggctctctgt acaaggttag ggagctaaca aagcagggat aaaaagtccg
      481 cctgatgcag gcacggttcg ttaaatcttt tgccattggt aacaaaatgg tgcgatgaat
      541 gttcaggcta tctttatctt gatggtggtt cagctgtttg gtaaagaaat cgctgtaaca
      601 atagaagaac tgcaggcagt actggtccca catcgtttgc tcagtcaact ggtgaagttc
      661 cgtagcgcgt aaattaatgc tttgcatgaa acgttactgg agtggatgaa agaatacctg
      721 catatatcat tactgcgttg gattaccagt acaacagatt gaatgaactg gacatgttga
      781 tagacggcat tgaaaagcag cttataagca tagtgcggca gagttaattg gagagcactt
      841 cgccttctga gtattaagta agcatggtgg tacctgcgga tactatctat tcactgtgct
      901 cggtcggcag ccttactgac caaggagttg ggggagttga taacaaagtt gaaaaaacag
      961 ctctcaacca aggtggttat tgtaacaata gcaaataagc ttgtgtgacc tgtatcacaa
     1021 taaccgatta tggacgtaaa tacgacaaaa atatgtcagt atcaggcggt attaattgct
     1081 gatgctgatg gacattaact cttaataaaa gggtggatgc tgaaaggttg ttatggtgac
     1141 ggacgtgatg acgacggaaa agagtatgag gttttggatt taaagttcgt tcggggaata
     1201 aggacccggt cagctaattc tcatagtagt tcgcattgct ggatgcaata ctgtgctgca
     1261 attactcgtc gatgtcggaa caacggaagc cttgcaaacg gggatgtatt catacaggtg
     1321 ttatgttata tggggtttta ttgcaggggg ctataccaag agaatatatg atatctcatc
     1381 agactgatgt gagggtaaat gagaatagag taaacgaaca aggttgtttt ttggcgagga
     1441 agcagatgta tgacaattct tgtggagctg ccagtttact ctgtgctgca aaggaattag
     1501 gggttgataa aataccacaa tacaaagggt ctatgtccga aatgacaagg aagagttctc
     1561 ttgatttgga taatcgttgt gagcgggatt tatacttaat aactagtggt aattataatc
     1621 cgagaataca caaagataat atcgcggatg ccggttattc tatgcctgat aagatagtca
     1681 tggctacgag gcttcttgga ttgaatgctt atgtcgttga agaaagtaac attttttcgc
     1741 aagtaattag ttttatttat cccgatgcaa gggacttatt aattggcatg ggatgtaata
     1801 tagtacatca acgagatgtg ttatcttcta accaaagagt tttggaagct gttgctgtat
     1861 cctttattgg agttcctgtc ggcctccact gggtgttgtg tagacctgat ggaagctata
     1921 tggatccagc tgtaggagaa aattattctt gtttttcaac tatggaatta ggtgcaagaa
     1981 gaagtaattc taactttata ggatatacta aaataggtat ttccatagtt ataactaatg
     2041 aggctttgta aattgtcgtt ttttatctgt gaaggagctt gaatattttg gggcgggggg
     2101 gcataacaaa tgatattact gagtacaaat ttagctatat tgggcggatt agttgtgtgg
     2161 gaagacgaaa tgtttctctt gaaataagtg aactccctca aggaatgatt gtgtgcttaa
     2221 atatttaagt gctggtaagt ttgagaaggc tgacatattt tgcagagcaa acagtgattg
     2281 acttggatga tatttttatt ttagtcactc gttgtaattg gagtatgctt tattttattt
     2341 gaaaatagat gtattttata cccgcaattt aaactgtaag ccctatgagg ggtatggata
     2401 ttataagtta ctctttgact aagcataact tttggggtta gaaaaataaa tctcttgtta
     2461 ataattaata tgattttaaa atcaaacttg tttataatta taaaataatg taagtcggtc
     2521 attttatggg aagattagga gaataaaaca gtaattatat ttgagaaatc tgctaatata
     2581 ttcttaaagc ttttttgata ctgtgaacga agtctgttaa tggtggattt tttgtttatc
     2641 accatgtaaa aaaattgagt agtaaaagcg ttgactgcta atcctcgctg tttttattga
     2701 aacgattaaa aatacgctga atattttcgg ccagtcactc atcttcacat aaaattttga
     2761 ccatcatgcc gcatccctta gttgcacaat tcattttcaa tgatatgaat attactaatt
     2821 tcaccagata attttctcgt ggtcagcagg gtatgagttt tcaccgtaat tttaaggaaa
     2881 cacagtgcga ggggattgaa taacagatag ccgacggaat taccccaccg gctgaaagtc
     2941 aagttcaacg aagattttta ttatctgaat tgggcagttt acatcagtgt tcgatgttta
     3001 tgaagacgat agatggcgag aaattatatc ccgaatagct tcatctattt ctctctgaac
     3061 tgttttgttt attttagata atgtaaatat tagagttctg tttgttttat tctcttttct
     3121 ttttatgtaa gtccgtttgt ctttagaaat aaataacgag tcaactttta gcgaagggtt
     3181 ttgctttctg tttttgcttt tctttatgat gtttaaaatt tcttttttat atatgtccgg
     3241 gggcaaattt gtatcaaggt cttttatttc ttcctttaat attggtagtg tagaactaag
     3301 agattcatta gccttttcaa ggcctttata ataattgaat aatatcttgt agtcattaaa
     3361 gttaagttct gacgcgattg gaaatagaga tattatttcc tgtggaacgc ttgctgcctg
     3421 aaaggcacga gtgactttcg cgcgagacag attctctttt ttggctatgt ctttatagga
     3481 catccctgat actttcagaa aattaagacc aataccaagt tctcggatgc tatgctcttt
     3541 tgctgtttgt atatcgtttg ctagttttct ggcatcaaga gtagatatat attcttttga
     3601 atatagaact tcaagatctg ctcctgcata tattgcagat gctcttctac gagtgccatc
     3661 cagaatttca attctaccat caatctccct tcctattaca gggaagaatt gttgtagctt
     3721 tatagttttt atgatatcgg ctaatgattc ttctgttagg gattcttgat cgcgtacatt
     3781 cgttttttgg ttgacgaagg ttaaatcttg gatcctttta tgaggaatct tggctttgat
     3841 aaaggaaact ttattaccag aatggagtgt aaactctttc ttttggcctt cctttatact
     3901 taacaagtcg ttgcacaaat ccaccatatc acaccctgtt tattcatatt gatttatatt
     3961 gacaaaagtt aaatgcagtg tgagcaagat tgtatgtaac tgttgatgat agtcaacggt
     4021 agtcttaaaa attactattt taatagctgg attgatgcaa tttatcttta ttggataaaa
     4081 gaataatatt cttgtggagc tctgatgtga gagctccata gatgaaaaaa tgcttatcaa
     4141 ttactatatt gattttgtac tatttttgac tacagaaata agatctccaa tagtaataat
     4201 gtgttctaat gcagattcat ctatacgtag attgaactca aaactaatca tatccagcat
     4261 ttcaatcaga gaaagagaat cggcagaaag atcttcaata aatttagttt cttcagatag
     4321 tttgctgtac gctataccat agcaaaaggc cactattgat aatatttttt cttttatcat
     4381 attttaatcc ttattgatat tctttaatac ttttgatagg gaagtggtta catcttttgc
     4441 tgctttatat atattattgt tttcagctgt aatatcattg ttatttttta aatcagatat
     4501 atcatctgta agtaattctg cagaggtatc gtcaatttgt gacaatacct tgctgagggc
     4561 gctagatact tcttttgcct tttcatagat cgcatgattt ttatcaattg tatctgcgtt
     4621 ggtttctgtt acgtagttta ttttatttgc atctgtaact tttaatttgt tgtttattct
     4681 gtgttctgtt gtggattttg taatgtcctt tgatttttcc tgtaactctc gttcttgtgt
     4741 tcctgattta gttccaatga gagatataag attacttagg gagtttgtta ctttttttga
     4801 agcttcaaat atcgtttccc gtgttaaata tgtgtctcca tttcttgata aaaaatcagt
     4861 ctgaagtact tttgaatcag tgtttgagtt gaatttattt atttgcgaag ctactctatg
     4921 agaaagagaa gcgctacttt tagttgtatc tgatgagtta tcattactgc ttgtcactcc
     4981 ttccgggtgg agaggggagg tggttgaaga gttctgtatg ttcagaggcc tgtgtccccg
     5041 agaaagagag ttatttgcag atctttcttg actactcgta gttaacaggt ctgaacctat
     5101 tccatccgaa ttatctggag tatggttgaa aaccttttct aaagctgaat tatttgcaaa
     5161 tgtctggaca gtatctgata tagagtgtgg tagaacggaa gttacttttt gaactagtgc
     5221 tctttgtgga tttagtaatg agtttccgtt acgggataat agatcgtttg tctgagaagt
     5281 aggagactgt gcatcatttt ctgggttctc gtgatagcta ttgtcatata ctctgttgtc
     5341 aaaaactcta ttatcgtaag ttctattatc attatttata tgatagtgaa ttacaggttg
     5401 ggatgttttc tcattggctc cgggggttgg tccgccatct ggcatatctt cgggaggtgt
     5461 agggaggcca tcttggatag gatgcgaatc ttttatatct tctaaaatat cattaaggac
     5521 ttttctcaac agctcaggaa acacggctac tgtatttaag tctcttgaca gtttacctat
     5581 accaaatcct ttactatcaa tagtattttt acgttgattg cgcaaactat caaaggcaag
     5641 ttttgcctgt gcgtcaatta aatcatctag tttgagagat gaaacagttt cattacttaa
     5701 ctttaattca gcaagaatat tattcttgat aaatgatgat acttcagtat tgaggagttt
     5761 ttgtcccaat gataaagctt caccagatgt gtgttgttgt gctaatagtg caagtctgtg
     5821 ctcaatgttt tgtcgtaatt gatggagttt cttattcttt gccgaccatg gtccaaaggc
     5881 agaatcttgg gataatttat cttttataat tagttccgaa tactgtgcga caattttttg
     5941 aagtcccttt tcgttttcaa aattaagacc attttgatgt actaaggagt acaaaacatt
     6001 tgaaaattcg agaatctcgc ttttggcata cgcattcatt gagttggaat aaatgcttct
     6061 taacacatct tctttccctg aggaagaaaa taaaaaatca agacatttct gattaaaaga
     6121 cgttgcaaag ttttcttttt cagacactga tgctggtgtt tttagagaag tttgcgaact
     6181 atggatatcg gatattttgc tttttaactc gctgtattct gttgatgatg gtgaagttgc
     6241 cttatataag aatgttggcg cttgagtatt atttacatta tgcatggcgc acctcagaaa
     6301 tggagaaaaa gtttatctgt atctgtacat gagcttattg tactactcaa aacctttact
     6361 aaattatcaa aaatactatt ggcattactg tatttttgaa ctaaagtttg aagattattt
     6421 ttcattgttt catcttcggc agagaatccg gcattccatg cctgatattt tgcattatct
     6481 agcacaacct cgccatttcc acctagatta tctaagcttt ttaacatatt gtctattggg
     6541 gtcatgttta tactgacaac atatcccccg tttttttgag ataccttgcc gattgttcca
     6601 cctaattctg taagccattt atttgcttgt tcctgactaa cagtattatt tgctggatat
     6661 agcggtttat ctttatattt ttccttgagt tcttccaatg cctttttaag cgagttgact
     6721 tgtaatttca cggagtttcc gtcgttacct ccgggagaga tccagccggc aagactggaa
     6781 agaacagcgc taaaatcttg atacatttga gtatatgaac taacggcatg ttcatatact
     6841 ttcagatact gttcattaat atcattgatg gagtttgcaa ttttagccca cagttctcta
     6901 tgagatatca tttgatctcc atcaagctcg gcttctttcg gggctgaatg taataattct
     6961 cttgcgtctt tattaattgg atattcgttc ctggaaagaa tatccaatag ttgagcggat
     7021 ttatttacat ccatgctaat ctgagatgaa tgtaatgcta tttcttctag cgaagtttta
     7081 gtcaacgttt tttgtgaaag ctctttcttt aatgccttat ttgttgttct gatattatga
     7141 agggtgtcgt taagcatagt aagggaactt acaggatgag aactggtcgt tgtttttata
     7201 tcagaattaa ctgtttcggt tgatgaaccg ttggtattgt ttggactgaa tgatgaggtg
     7261 gaaatactat tagtcagagt tgttatattc atggttattt ccttacagga agagccatat
     7321 attattatgg ctcttggata tattaagctc gaatgttacc agcaatctga ctggctgccg
     7381 aattccttga ttgggtgatg ctgtcaatta tattcaataa tttttgtatt aattgatttg
     7441 tcgcttggga tgcttcttta gatacttggg atgcttcctc tgcttgttta ctgctggcct
     7501 gactgattag ttgttcttct tcttcaagag cagatgcata acgccctcct gatgtggata
     7561 tattaccagc aacggctgat gtttccattg ttgcacggcc aatgttttgt ttttgctgcg
     7621 cagaaagggt attgagctcg taagttcttc tctgggtgtc aattttatcc tgcaaagaaa
     7681 tatcgggaga actaagagaa gttttatgtt cagttgacag ggatatatta tctggcgcca
     7741 gtttattttt acctaaaaat tttgtgctag agttagtttg tggtgaggtg atatttgtat
     7801 ctatttgttt atttaaccct aatttagaac ctgcaagctc tttttcaaga gattgagcag
     7861 tggcaaggtt ctttcttaag gctccttttt ggtcgctaat ccctgaatgc gtttttttag
     7921 caccgatacc cgttatacct acttgtgtga ctgctccagt aatgcttgat gatagggctg
     7981 ccaggccttg ccgaacaatg ttctctgcag ctgattttgt agcatcgaac gcaatcaatg
     8041 acaattgaga gcccaattta ttttctgcag tgcggagagc agaaagtaga acggctacac
     8101 taataattaa agaaacagca ttagaagaaa gactggaaat atccagagtg ttctctggag
     8161 taagggtgtg actcatctct gaaagatctt tcatcttttt gtcagtaagg cgagcaatga
     8221 tttctgaatc atgctgaacc tgctctgata actttaaaag ttgatcatca tttaatgtgg
     8281 ttgttaatac gggattttta ccgacattaa gtggaatatg cgctgcaatc tgctgataat
     8341 tttgtgattt ttgtgtttcg gaagaacttt gagtttgttt tgtggatata tctgtatata
     8401 aaatctgggt tggttttgtg ttttgaattt ccataacatt ctccttattt gtatcaagca
     8461 gtagtttgtt gcaaaattgc ttttgcaaca tcagttctat tagcctgaga gttggacatt
     8521 gaggctaata gatctgcaat tacttcctgc aattggccga atttttctat tgcttcactg
     8581 atatattttg acagttgttc aacttgatat ttcgataatg tcaggtctgc tagatttgtc
     8641 gacgcgctgt tctggaaaac agcagaagcg acacttcctc ccgcttgtgt ggcagagtta
     8701 acactttctc ctaataaaac tgcttggttt aaatgggtgg aaataatttg tttggatatt
     8761 acttcatcac ccgctgcacc aagaaattta tttaatctgg caacagcatt agtgattaaa
     8821 tcgtccagtt gagaagaaaa attcttgaga aactttggta taaggtctgt gagggtttta
     8881 cctattattt tgccaatatt ctctgcaagt tttgctgctg cctgtttacc aacagtggct
     8941 acgagaacga ctgctgcaac taggacaaga gcgcctgcga ttgcccccag aatagagcca
     9001 atcattttgg cttttttcga gtcgacgccc aagccttcga gcatttttgt aaatgcatct
     9061 gaaaggagtt tgattaaggg ttcaatgact gctttcatga tcggattcag ggcttgttcc
     9121 atgaaggaat tgccggtcgc tgcttgtact atagcatccg taaccataag agctaaacca
     9181 acagctgcca gtgctagaga ggctcctcca gaaaaagctg ctgcaacaac actaacgata
     9241 gttaaaagtg ccccaagtat tttcccaaca caacccatta ctctgttgag ttcttctgct
     9301 ttacgtactt cagcagcata ctcatcagat tttctctcca tttcagtttt tcttgattct
     9361 tggagagact ggaatagagc cagatcattt tttaaagatt cttcattatt ttttccaact
     9421 agttgaataa aggttgccat caattgagta acactggcaa gtccggttaa tgatttctgc
     9481 tgggttgata gctgttcagc agatgctgtg tttgaaaatg cagaaaaaga gtctatttct
     9541 ttttcgagtt gcattgattt atctgtaagt ttgctatgaa ttgacagggt tttctgctca
     9601 atcaatgtcc tgtctttaac tgctgcgtct tttttgatag tgagttgtat ttcttcccgg
     9661 cttaattttt tcttttctgg tgactctggg tcgagttcgg ataatcttgt ttgaatttgg
     9721 ttaattttat tttctaggtc ttttatttta gaatctgcgt tttttagttt attaatttgt
     9781 ttttcatagt ctctggttag tccttcagtt tcagatagaa gagtgttaat tttatcggag
     9841 aattctaggt ttttttgctg tcttgcctgt tgctgggact tccaagcagt aattttattt
     9901 gttaatgcag ttaaagattt ttcaccgagt atttgaataa ggtttccaat taaaagcgtt
     9961 agttgggaac ttgcatttaa tgactttggt gctttaagtt cagggattaa tatatttgaa
    10021 gttgagtgtg cattagtttt attaatattt tggttagcag taagatcagc aattgtaaga
    10081 gaaaataatt tattagctgc atcatttgca gcttggatag tattgtctcc aagctcagtg
    10141 gaagccaata ttttggcaag aggaaaacca gtggttgtgg tgcttacatt atgcataata
    10201 attactcctt gatatcctga attgcgtcca agtatgattg tgcttttatt tttaattttt
    10261 catcattgct gtgttgaatt acgagttcga agcactcttt agcttttaag ggggctttca
    10321 accgaagctg gcattgtcca gtatggaata ctggtgtata gtcatttttt cctaatgcaa
    10381 aagcgacagc ataaaggtct gctgcttgtt ggaactgttc ttttatctga taaatagctg
    10441 cgagtcccat aatgtagtct acattgtaaa agtcgtatat acataaaaat ctgaagaaaa
    10501 cttcagcttc ctctattctt cctttgttgt aaaagtcata agcatatgaa taaatgtcat
    10561 ccatcatatc atcaggaatt gcattaatat ctttcagtgt agcgccagag ttaattgcat
    10621 caattactgc agtagagatg ctttcatttt cagtgatatt taaagacata aggtctcctt
    10681 tttgtctgct ttactttaat aagtataaga tttaatataa aagatttaat ttgtattgct
    10741 ttgacggtat acagccttta gaacctctgc gcttatttct atttgtaaaa cttttgttga
    10801 ttcattaaat attttatcgt agacggaact ggatactaga tttgtaagat ccttcatttg
    10861 tgtgttaaac aaatccatgt cactgctata gcgttttaaa atctttgctc tgaggccaga
    10921 tgaggcaatc tcgtttagtg ctgtatgact gatagatgtt tgtgctgatt tttcatcaag
    10981 ttttatatcc gggaattttt tttcaatagc tgcaaatatg ttttttttgt tatctgaggt
    11041 caaaacaact ccattgtgta tggccatttc atctatactt ctgtttatca ttgaagagac
    11101 ataagaggtt cgctcttggc tcatccaaca aaatagttga ttccccgaat tactatcttt
    11161 tattttgtct atgacgctgt ccctgatttt atttatattc accgggcatc caaaattctt
    11221 ctgaggaaag cgttgtttta agttaaaaac agataatatt ttctttacaa gcttattgcg
    11281 acttaacgaa tcaaatgatg ggcgaggaat cgcgaattca acctgtggaa gtattttgtt
    11341 tagaatttgc atgatacccc ctatatgtta gttcacttct gaagtgatgt ttgcaatggt
    11401 tagtttaata caaaataaaa attcatacaa agattcaatg agttcatcag gtgaaagata
    11461 ttttttgtag tgtttcttgg tcaaaacaca ttgatgagta tcagggagaa gacagataga
    11521 agaaccataa tctctactca aagagtagtt aaggatgagt atgtgttctg gattaataat
    11581 attttctgta aaaagttcat gttcattaag aagtccggtc aaaaagatgt attcatcatt
    11641 atctgttata caaacaggta tttgttcttc gagcaatata aaggcttgat ttttatcgtt
    11701 aaaatcaagc tttgcacctg ttatttcata aagtttatca tatagtttgc gacacatttc
    11761 tatggccttc tatatattag aatgagagtt attcaataaa agcttattga tctgtaataa
    11821 gtcgtgaatg taaagtttgg ttccaagatc tggcgattta agagaattgt aataatcaat
    11881 aatttttatt gatttttcga taatttcttc ttttttatct gttgcttcgg tttttaattc
    11941 gtttaataca cgagtcatat tgctgggtgc tgattttact aagtatgagt ggcaagcgtt
    12001 gatgaccaca tcaaagtctt ctgaagatac attttcagta ggatgtttgt aaagtgaaat
    12061 tgataaaggc ttgaatgttt ttggtgtgtg gataaggtta attttattga aacttttaag
    12121 atagctgcgt cgtaattgag tcaatctgga aagatcaaaa tcagattcaa tatacttcat
    12181 tatttcgtta taagactgat tgagactttc aatgcgttgc ctaactttat ttgcatacgc
    12241 gtatacatcg ttaggtgtag caaccaattt aacgtcaggg agtggaaagt atatttcaga
    12301 atttcctgct ttaagaacat taagtgccat accagcacag ttttcagatt tactgattaa
    12361 tttataattt gccaattgtc tgaaagcatc tgctttcttt ttgcatataa attttgacat
    12421 gtttgtttca tcaaggccaa aaaggttatg gctgatttta ccatcctttg ttttattatc
    12481 acctgataat ggaatgtata ctttatcagc actgaccccc caataaccat aggggtcttt
    12541 tttttgatta gctctgggaa aaaatatttt tttgttgtta ttttgatctt gttttgaatt
    12601 taataagttt ctaacctctt ggcctgcatc aagtctttcg gctgtttggt ctgaaatcat
    12661 atttagtttg tcttgcttat aggtatcgac actgtaacct aagcgttttt caaaaaggta
    12721 gtttatttct gtatcttttg taacatcccc gccgggatac cagctgagat aggtatgagt
    12781 tggttcctgt agtaaattgt tttttatact gatactagta tgccctaaat gggttatgcc
    12841 tctatttccc cagcaacgaa aaactaaatg tctttcaaga tttttggctg taccgatgcc
    12901 atgaaaacgc tctgcggtat tcaacattaa tttgatgtct ctacctgtga aagagtgttg
    12961 aattgagttt ttagctgctt cataggcggc aaacgcactt tctgcagatt tttcgctagc
    13021 taatgtattg aggaaaagca ataaggattt ttgattctct agtcgataat tttttagttg
    13081 ttcatttaat atctgtgaat ttatctttgc attaatagtg cttaatgttc tgaaaaagag
    13141 atcctttagg ttgaatttct gtgctatcaa tataggtcca tgctctgtat cttcaacgcg
    13201 tataggccct tttgtattgc ttgcgtcaat gaaattgcta attttgaggc tcatacttta
    13261 ttaactctcc attacttggt gattgtgggg atgtaaaatg tatttggcat ataatatatt
    13321 caattaataa atttagaaac ttgagcctgt taacataatc aaattttctt ttgctgtaca
    13381 taatatgtac ctcgtgagca tatgtagtgc tcgtttcatc atgaaatccc acaagataaa
    13441 gtgcctgatg tatcaggctc ggagtgttat agaaaaagag agaaccctgt tgaataagta
    13501 tcttttttgt tgtaaaacga ggtacaaaac tgcaatcaat gccccattta tgactgagga
    13561 taattaaatg cacataacta atttgggatt gcatcaggtt tcatttcaaa gcggagattc
    13621 ctataaaggc gcagaagaaa ccggaaagca caaaggtgta agcgtgattt catatcaaag
    13681 agttaaaaat ggagaaagaa ataaggggat cgaagcgctt aatcgattat atttacagaa
    13741 ccaaacaagc ttaacaggaa aaagcctttt gtttgcgcgt gacagagctg aagtttttta
    13801 cgaggcaata aagttggcag gtggtgatac gtcaaaaatt aaagccatga tggaacgatt
    13861 agatacctat aaacttggtg aagttaataa aagacatatt aatgagctta ataaagtaat
    13921 aagtgaagag ataagagcac agctaggcat taaaaataaa aaggaattgc aaacgaaaat
    13981 aaaacaaata tttactgatt atttaaataa taagaattgg ggaccggtcg acaagaatat
    14041 tagtcatcat gggaaaaatt atggttttca attaacacct gcctctcata tgaagatagg
    14101 gaataaaaat atatttgtca aagagtataa tggaaaagga atttgttgtg cttctacgag
    14161 agagagtgag catatcgcga atatgtggct ttcaaaagtg gtggatgacg aggggaagga
    14221 aattttttcg ggtatcagac atggagttat atcagcctat ggattgaaaa aaaactcatc
    14281 tgaaagagct gttgctgctc gtaataaagc tgaggagtta gtaagcgcag cattatatag
    14341 cagaccagag ttattatcac aggctttgtc tggtaaaaca gtagatttaa agattgtttc
    14401 gacttctctt ctgacgccga ccagtttaac cgggggagag gagagtatgt taaaagatca
    14461 ggtgaacgca ttaaaagggt taaactctaa aagggggaag ccaacgaaat tattaattcg
    14521 gaatagtgat ggtcttctga aagaagtaag cgttaatcta aaagtggtga catttaactt
    14581 tggtgtaaat gaattagcgc taaaaatggg cttaggctgg aggaatgttg acaagcttaa
    14641 tgatgaatca atatgttctt tgttggggga taatttcctc aaaaatggtg tgattggagg
    14701 ttgggctgca gaagcaatag aaaaaaatcc accatgtaag aatgatgtga tatatctggc
    14761 taaccagata aaagagatta taaacaagaa gttacaaaaa aatgataatg gagagccgta
    14821 taaattgcca caaagaatga ctctattggc ttatactata ggtgctgtac cttgctggaa
    14881 ttgtaagagt gggaaggaca gaacaggtat gcaggatgct gaaattaaaa gagaaataat
    14941 aagaaaacat gaaacaggtc agttttctca attaaatagt aaattatcct cagaagagaa
    15001 aagattattt tctactattc taatgaatag tggtaatatg gaaatccaag agatgaatac
    15061 tggtgttccc ggaaataaag tcatgaaaaa attgccgcta tcttcgttag agctatctta
    15121 ttctgaaaga ataggggacc caaaaatatg gaatatggtg aaagggtatt cgtcatttgt
    15181 ataagaggaa tatatggaag atttagcaga tgttatttgc cgagccttgg gtatcccttt
    15241 aattgatatc gatgatcaag caatcatgct tgatgatgat gtgctaatat atattgaaaa
    15301 agaaggagat tcaataaatt tattatgccc tttttgtgca ttgcctgaaa atataaatga
    15361 tcttatatat gcgttgagcc taaattactc agaaaagata tgcttagcta ctgatgatga
    15421 aggcggaaat ctaattgcgc gattagatct taccgggata aatgaattcg aggatgtata
    15481 tgttaataca gaatattata tttcgcgtgt gcgttggctg aaggatgaat ttgcgcgaag
    15541 aatgaagggg tattaatgtc ccgtttcgta tttattttgc tgtgttttat cccatattta
    15601 gggagagctg attgttggga taaggctggt gaaaggtaca atattccatc aagtttgtta
    15661 aaagcgattg cggaaaaaga atccggattt aataaatctg cagtcaatgt taataacaat
    15721 ggaagtaaag attatggtat aatgcaaata aatgactttc attctaaaag acttagagaa
    15781 atgggatatt ctgaggaaat gctaattagt catccatgtc tttctgtaca ttatgcagca
    15841 aagttattaa atgagtttat gatgatgtat ggaagagggt gggaggctgt tggagcctat
    15901 aatgcaggaa cgtctccgaa aaagaaaaaa gaaagattaa agtacgcaga ggatatctat
    15961 agacgctatt tgcggatagc agcagagagc aagcagaata atcgaaggat ataagaggat
    16021 ttatgtctga ggcaaagaac tcaaatcttg caccattcag gttattagtg aaactcacaa
    16081 acggtgtcgg ggatgagttt ccgttatatt atgggaataa cttaattgtt ctcggacgga
    16141 cgatagaaac acttgagttt ggaaacgaca atttcccaga aaatattatt cctgtaacag
    16201 atagtaaatc ggatggtata atttatttga ccataagcaa agataatatc tgtcaattta
    16261 gtgatgaaaa aggtgagcaa attgatatca attcacaatt taattcattt gaatatgacg
    16321 ggatttcttt tcaccttaaa aatatgaggg aggataaatc gagaggccat atactaaacg
    16381 gaatgtataa gaaccactcg gtgttttttt tctttgctgt tattgttgtg ttaattataa
    16441 ttttttccct ctctttaaag aaagatgaag taaaagaaat tgctgaaatt atagatgata
    16501 agagatatgg aatagtcaac actggacagt gcaattatat tcttgccgaa acacaaaatg
    16561 atgcggtatg ggcgtctgta gccttaaata aaaccggatt taccaaatgc agatatattc
    16621 tggtgtcaaa caaagaaata aatagaattc aacaatatat caatcaacga ttcccattta
    16681 tcaatttgta tgtgttgaat ttagtgagtg ataaagcgga attactggtg tttttaagta
    16741 aagaaagaaa tagttcaaaa gataccgaac ttgataaatt gaaaaatgct ctgattgttg
    16801 aatttccgta tatcaaaaat attaaattca actatcttag cgatcataat gcaagggggg
    16861 atgctaaagg tattttcaca aaggtaaatg tccagtataa agagatttgt gaaaataata
    16921 aagttacata ttctgtcaga gaagaactga cagatgaaaa gcttgagctc attaatagac
    16981 tgatttctga acataaaaaa atatatggag atcagtatat tgagttttct gtacttttga
    17041 tagatgatga ttttaaaggt aaatcatatc ttaacagcaa agacagttat gtaatgttga
    17101 atgataaaca ctggtttttt ttagataaaa ataagtgagg ataaaatgag tgttacagta
    17161 ccgaatgatg attggacatt gagttcatta tctgaaactt ttgatgatgg aactcaaaca
    17221 ttacaaggtg aactaacatt ggcactagat aaattagcta gaaatccttc gaatccacag
    17281 ttgctggctg aataccaaag taaattatct gaatatacat tatataggaa cgcgcaatcc
    17341 aatacagtga aagtgattaa ggatgttgat gctgcaatta ttcaaaactt cagataatag
    17401 ggagcattca tgaattacat ttatccagtc aatcaggttg atattatcaa agccagtgat
    17461 tttcaatctc aagagatatc aagtctggaa gacgtcgtgt cggctaaata tagtgatatt
    17521 aagatggata cagatattca agtatcacaa ataatggaga tggtaagcaa tccagaatca
    17581 ttaaacccag aatctttggc caagttacag acgacgctct caaattattc aataggagta
    17641 tcattagctg gcacgttagc aagaaaaaca gtttcggctg ttgaaacttt attaaagtct
    17701 taatttatat gattaggtat aaaggtttta ttttattctt gttgctgatg ttgattggat
    17761 gtgagcaacg tgaagagtta atttctaatt tatctcaaag acaggcaaat gaaataatat
    17821 ctgtgctaga acgccataat attactgcta gaaaagttga tggaggtaaa caggggatct
    17881 cggtacaagt cgaaaagggg acatttgcat cggcagttga tttgatgcgc atgtacgatt
    17941 tgccaaatcc ggagagagtt gatatctcac aaatgtttcc tacagattca ttagtgtctt
    18001 ctccaagagc tgaaaaggcc cgtttatata gtgctattga gcaacggctg gaacagtctt
    18061 tagtttctat tggtggtgtt atttcggcaa aaatacatgt tagctatgat cttgaagaaa
    18121 aaaatatatc ttcaaaaccg atgcatatat cagtaatcgc tatatatgac tcaccgaaag
    18181 agtctgaact attagttagt aatattaagc gatttttgaa aaacaccttt tctgatgtta
    18241 agtatgaaaa tatatctgtc atattaactc cgaaagaaga atatgtttat acaaatgtac
    18301 aacctgttaa ggaagttaaa tcggaatttt taacaaatga agtaatatat ttatttctcg
    18361 ggatggctgt actagttgtc attcttttgg tatgggcatt caaaacaggg tggttcaaga
    18421 gaaacaaaat atgataagaa tggatggaat ttataaaaaa tatctttcaa taatttttga
    18481 tccagcgttc tatataaata gaaatcggtt gaatttgcct tctgaactgt tagaaaatgg
    18541 cgtaatcaga agtgagatta ataatctcat aattaataaa tatgatctaa attgcgatat
    18601 tgaaccttta agcggggtaa ccgctatgtt tgttgccaac tggaatttac ttccagctgt
    18661 tgcttatttt atcggtagtc aagagtctcg gttaattaat catagtgaga tggttattag
    18721 ttattatggg ggcaaaatca gtaagcaagg ggaggccgct attcgaagtg gtttttggca
    18781 tcttattgcg tggaaggaaa atatatctgt tggtatatat gaaagaataa acttattatt
    18841 taatccaatt gctttggagg gaaattatac gcctgtagaa agaaatttgt cccgattaaa
    18901 tgaaggtatg caatatgcaa aaaggcactt taccggtatc cagacatcat gcctatgatg
    18961 gtgttgttat aaagagaatt gaaaaggaat tatgtaaaac cataaaagat agagacactg
    19021 agagtaagag gaaagcaatt tgtgttataa aggaggctac gaaaaaagct gaatcactgc
    19081 gtatagatgc tgtatgtgat ggttatcaaa ttggtataca gactgctttt gagcatatta
    19141 ttgactatat ttgtgaatgg aagttaaagc aaaatgaaaa cagaagaaat attgaggact
    19201 atataactag tttgctttca gaaaatttac atgatgagag gatcattagc actttattgg
    19261 agcagtggct tagttcgttg cgtaatacag taacagaatt aaaagtggtt ttgcccaagt
    19321 gtaatttagc actgagtaaa aagttagaac ttgatctcca taaatatcgc tcagatgtca
    19381 agattatttt aaaatattct gaagggaata attatatatt ctgtagtggt aatcaagtag
    19441 tggagtttag tcctcaggat gtaatatcag gtgttaaaat agaacttgcg gaaaaattaa
    19501 caaaaaatga taaaaaatat ttcaaagagt tagcacataa gaaactgaga caaattgcag
    19561 aagacttgtt aaaagagaat cctgttaatg attaatcaaa taaatgcaag caatgctctg
    19621 cagcaaagat taaatagtga agaatttgtt aatttaaatg agagattatc atcttcgcaa
    19681 tcatttgatg aggatataat ctatgaaata atgcaatatt tctcacagag tgaattaaat
    19741 tccatagata atgatgaact gcacaataag attgaacaat tattcaattc acgttttcct
    19801 tatcttacag ctgcacaaaa aagttcactt cttaataaac tcatagatgc aaatcaatat
    19861 gtcgatttgc atgagggatt ttatgcatca ttgtccatat ataacaatat tgatttctat
    19921 attaagacta caaccttcga ttcattaatt agtgtctttg aagcagggag agaggcagat
    19981 gattcgacat ggtagtaata agttgaaaat atttatttta agtatattgc tattaacact
    20041 gagtgggtgt gctttaaagt catcatctaa ttctgaaaaa gaatggcata ttgttcctgt
    20101 aagtaaggat tatttttcta ttccaaatga tttattatgg tcgtttaata caaccaataa
    20161 aagtataaat gtttactcta aatgtattag tggtaaggcg gtttatagtt ttaatgcagg
    20221 taaattcatg ggcaacttta atgttaagga agtagatggg tgcttcatgg atgcacaaaa
    20281 gatagctata gataaactat tttctatgct gaaagacggg gttgttttaa aaggtaataa
    20341 gataaatgat accatcctta tagagaagga tggggaagtt aaattaaaat taattcgagg
    20401 gatataattg tattgtgagt aaatataaag gtctaaatac aagtaatatg ttttacattt
    20461 actctagtgg acatgaacca gttaacgttg agcttgtaaa agataaagaa cgtaacataa
    20521 ttgagctggc tccagcatgg aagggttttt ttttgtccga aatcaaaata taaaattcag
    20581 tgataatgtg aactatcatt atcgctttaa cattaatagt tgtgcaaaat ttcttgcttt
    20641 ttgggactat ttcagtggtg ctctagtaga gcatagccat gctgaaaaat gtattcattt
    20701 ctatcatgag aatgatcttc gggatagttg taataccgaa tcgatgcttg ataagttaat
    20761 gttaagattc atcttttcat ctgaccagaa tgtaagtaat gcactggcta tgatacgaat
    20821 gactgagtca tatcatttgg ttctttatct tctgagaact attgagaaag aaaaggaggt
    20881 aagaataaag agtctgacag agcattatgg tgtgtcggag gcttatttta gatctttatg
    20941 tagaaaagct ttaggggcaa aagtcaaaga acaactgaac acttggcggt tagtaaatgg
    21001 tttattagat gtatttttgc acaatcaaac aatcacttct gctgccatga ataatgggta
    21061 tgcatcaaca tcccattttt caaatgagat aaaaacacga ctaggctttt ctgcaagaga
    21121 attatcgaat attacttttt tagtgaaaaa aataaatgaa aaaatttaat attaaatctt
    21181 tgactctctt gattgtattg ttaccattga tagttaatgc taataatatt gactctcgtt
    21241 tattagaaca aaatgacata gcgaaatatg ttgctcaaag tgatacggta ggatcttttt
    21301 ttgaacgttt ttctgcgtta ttaaattatc ctattgttgt tagcaaacaa gctgcaaaaa
    21361 agagaatatc tggggagttt gatttatcta atccagaaga aatgcttgaa aaactgacat
    21421 tacttgtggg actcatatgg tataaagatg gaaatgcatt atacatatat gactctggag
    21481 aattaataag caaagttatt ttgctggaga atatatcact taattatctt attcagtatc
    21541 tgaaggatgc taacctttat gatcacagat atcctataag aggaaacatt agtgataaaa
    21601 cattctatat atctggtcct ccggcattgg ttgaactggt agctaataca gcaactttgc
    21661 ttgataagca ggtttcttct attgggacag ataaagttaa ttttggggtt ataaaattaa
    21721 aaaatacatt tgttagtgac agaacttata atatgagagg ggaggatatt gtcatcccag
    21781 gggtggctac tgttgtagaa cgtttattaa ataatggtaa ggctctgagt aacagacaag
    21841 cccaaaatga tccgatgcca ccctttaata ttacacagaa agtatctgag gatagtaatg
    21901 acttttcatt tagttcagtt accaatagtt ctatattaga ggatgtatca cttattgcat
    21961 atccagaaac caattctata ttagtcaaag gtaatgatca gcaaatacag attattcgcg
    22021 atattatcac tcagttagat gtagccaaac gacatataga attatcatta tggataatcg
    22081 atattgataa atctgaatta aataaccttg gtgttaattg gcaaggaacg gcttcatttg
    22141 gtgattcgtt tggtgcgtca tttaatatga gctcatcagc atcgataagc actttggacg
    22201 gcaataagtt tattgcttca gttatggcat taaatcagaa aaagaaagca aatgtagttt
    22261 caaggccggt gattttgaca caagagaata ttccggctat atttgataat aacaggactt
    22321 tttatgtgtc attagttggt gagcgcaatt cttcgttgga gcatgttacg tatggaacat
    22381 tgataaatgt tatacctcgc ttttcatctc ggggacaaat tgagatgtct ttaacgatag
    22441 aggatggaac aggaaatagt cagtcaaatt acaattataa taatgaaaat acatctgtcc
    22501 ttccagaagt tggaaggacc aaaatcagta caatagcgag agttccacaa ggcaaaagtt
    22561 tgttgatagg tggttatacg catgaaacga actctaacga aatagtatct attccttttc
    22621 tctcctctat tcctgtaatc ggtaatgtgt tcaagtataa aacaagtaat atcagtaata
    22681 ttgtaagggt ttttctgatt caaccccgtg agataaagga gtcaagttat tataatactg
    22741 cagaatataa aagcctcata tctgaaagag aaatacaaaa gactacacaa ataatcccat
    22801 ctgaaactac tttgctggaa gacgaaaaat cattggtttc atacttaaat tactaactat
    22861 aaagtaggtg atgtatgctt gatgttaaaa atacaggagt ttttagctct gcattcattg
    22921 ataagttgaa tgcaatgaca aattcagatg atggagatga gactgctgat gcagagcttg
    22981 attctggctt ggctaatagc aagtatattg actcatctga tgagatggct tccgctcttt
    23041 cgtcatttat aaacagaaga gaccttgaga aactgaaagg aacaaatagt gatagtcagg
    23101 aacgtatttt agatggggaa gaagatgaaa ttaatcacaa gatttttgat ttaaagagaa
    23161 cgttaaaaga taaccttcct ctagatcggg attttataga cagactaaag agatatttta
    23221 aagatccaag tgatcaagtc ttagcattaa gggaactttt gaatgaaaaa gatcttactg
    23281 ctgaacaagt cgaattatta actaaaatta ttaatgagat aatatcaggt agtgaaaaaa
    23341 gtgttaatgc tggaataaat tcagctatac aggctaaatt atttggcaac aaaatgaaac
    23401 ttgaaccaca gcttttgcgt gcatgttatc gtggttttat catggggaac atatcaacaa
    23461 cagatcagta tatagaatgg cttggtaatt ttggttttaa tcacagacat acaattgtga
    23521 attttgtaga gcagtcactg attgtagaca tggattctga gaaaccgagc tgtaatgctt
    23581 atgagtttgg ttttgtgtta tctaaattaa ttgcaattaa gatgattaga acttcagacg
    23641 taatttttat gaagaaactg gaatcctcaa gcttgctaaa agatggcagt ttaagtgcag
    23701 agcagctatt gctaacttta ttatatattt ttcaatatcc aagtgaaagt gagcaaattc
    23761 ttacttctgt tatagaagta tcacaagcca gtcatgagga ttctgtagtg tatcaaacat
    23821 atctatcttc tgttaatgaa agtcctcatg atatatttaa aagtgaaagt gaaagagaaa
    23881 ttgcgatcaa tattctacga gagcttgtca caagtgcata caagaaagag ctttctagat
    23941 aacaggagat aaaagtgatc cagtcttttc ttaagcaagt aagtactaag cctgaattaa
    24001 ttatattagt gcttatggtt atgatcattg ctatgttgat cataccatta cctacatacc
    24061 ttgttgattt tttaataggg cttaatattg tacttgctat tctcgttttt atgggatcat
    24121 tttatattga aagaatattg agtttttcta cattcccttc ggttttgttg ataacgacat
    24181 tatttcgtct cgctttatca ataagtacta gtcgactaat tttagttgat gcggatgcgg
    24241 gtaaaattat tactacattt ggccagtttg tcattggtga tagtctggct gtaggttttg
    24301 ttattttttc aatagtaact gttgtgcagt tcattgttat aacaaaagga tctgaaaggg
    24361 ttgcggaggt agctgctcgc ttttctcttg acggtatgcc ggggaaacaa atgagcattg
    24421 atgcggattt aaaggctgga attattgatg ccgcaggagc taaagaaaga cgcagtattc
    24481 tggaacgtga gagtcaattg tatggttcat ttgatggagc aatgaagttc attaaaggtg
    24541 atgcaattgc tggcatcatt attatttttg tcaatttaat aggaggtatt tctgttggta
    24601 tgagtcaaca tggaatgtcc ctatctggtg cattatctac ttataccata ctaactattg
    24661 gtgacggatt ggtctctcag attcctgctc tgttaatttc tataagtgca ggatttatcg
    24721 ttacaagagt taatggtgat agcgataata tgggacgtaa cataatgtcc cagatatttg
    24781 ggaatccttt tgttctcatt gttacatcag ctcttgcctt ggcgataggg atgttgccag
    24841 gttttccgtt ttttgttttt ttcctgatag cagttacttt gacggcttta ttctattata
    24901 aaaaggtcgt agaaaaagaa aaaagtctgt ctgagtctga ttctagtggc tatactggta
    24961 catttgatat tgataatagc catgactcat ctttggcaat gatagaaaat ctggatgcta
    25021 ttagttcaga aaccgttcct ttaattttat tatttgccga aaataagata aatgcaaatg
    25081 atatggaagg tcttattgaa aggataagaa gtcagttctt tattgattat ggtgtcaggc
    25141 ttccaactat tttatataga acaagcaatg agcttaaggt cgatgatatt gttttgttaa
    25201 taaatgaggt gcgtgccgat agttttaata tatattttga caaagtgtgt attacagatg
    25261 aaaatggaga tatagatgct ctaggtattc ctgttgtttc aacttcatat aatgaacgtg
    25321 ttatttcttg ggtagacgtt tcatatacag aaaatctaac taatattgat gctaaaatta
    25381 aaagtgctca agatgaattt tatcaccagt tgtcacaagc tttattaaac aacataaatg
    25441 agatttttgg tatacaagaa acaaaaaata tgttagatca gtttgaaaat cggtatcctg
    25501 atctattaaa ggaagtcttc cgacatgtga ctatacagag aatttctgag gtattacaaa
    25561 gattgcttgg agaaaatatt tctgttcgca atttaaaact tattatggag tctttggcgc
    25621 tttgggctcc cagagaaaaa gatgtcataa cattagttga acatgtccgt gcatcacttt
    25681 ctaggtatat ttgtagtaaa atagctgttt ctggtgagat taaagttgtg atgctttccg
    25741 gatatattga ggatgcaata agaaaaggga taaggcaaac ctctggtggc tctttcttga
    25801 atatggatat agaggtttcg gatgaggtaa tggaaacttt agcacatgct ttgagagaat
    25861 tgagaaatgc aaaaaaaaat ttcgttcttt tggtatcagt agatatacgt aggtttgtta
    25921 aaagacttat agataacaga tttaagagta tactcgttat atcgtatgct gagattgatg
    25981 aagcatatac cattaatgta ttaaagacta tttagtgagg tttaaatatg agtaacatta
    26041 atttagttca attagttaga gatagtcttt tcacgattgg ttgtcctcca tcaattatta
    26101 cagatttaga cagccattca gcgattacca tttctttgga tagtatgcct gctattaata
    26161 tagctcttgt caatgagcag gttatgttat gggctaattt tgatgcacca agtgatgtaa
    26221 aacttcagtc atctgcttat aacatattaa atttaatgtt aatgaacttt agttatagca
    26281 ttaatgagct ggtagagctt catcgttctg atgagtactt acagttaaga gttgttatta
    26341 aagatgacta cgttcacgat gggattgttt ttgcagagat tctgcatgag ttttatcaga
    26401 ggatggaaat cttaaatgag gtcttataat caatgagcta tacaaaattg ctcactcaat
    26461 tatcttttcc taatagaatc tcggggccaa tcttggaaac aagtcttagc gatgtttcga
    26521 ttggtgagat ttgtaacatt caggctggaa ttgaaagtaa tgaaattgtt gcaagagctc
    26581 aggttgtagg atttcatgat gaaaaaacaa tattaagctt gattggaaat tctcgtggac
    26641 tttcacggca aacgttgatt aagcccactg cccagtttct tcatacgcaa gtcggccgtg
    26701 gattattggg agcagtagtc aatcctttag gggaggttac tgataagttt gctgttacgg
    26761 ataacagtga aattctttat cgacctgtag ataatgctcc tccgctatat agtgaaaggg
    26821 ctgcaattga gaagcctttt ttaacaggta ttaaggttat tgattcttta ctcacgtgtg
    26881 gtgaaggaca gcgaatgggg atttttgcgt cagctggttg tggcaaaact tttctcatga
    26941 atatgctcat tgaacatagt ggtgctgata tatatgttat tgggttaatt ggtgagcgag
    27001 gtcgagaggt tacagaaacg gttgattatt tgaaaaactc tgagaaaaaa agcaggtgtg
    27061 ttttagtata tgcaacttcg gattactctt cggttgatcg ttgtaatgct gcatatatag
    27121 ccactgctat agccgaattt tttaggactg aaggacataa agtagcgctt tttattgatt
    27181 cattaacaag gtatgccaga gcattacgtg atgtggcctt agccgctgga gaatcacctg
    27241 ccagaagagg ctatccggtt tcggtttttg atagcttacc cagacttctt gaaaggccag
    27301 gaaagttaaa ggcaggtggc tctattactg cattttacac tgttcttttg gaggatgatg
    27361 attttgctga tccattagct gaagaggtaa gatccatttt agatggacat atatatttga
    27421 gcagaaatct agcccaaaaa ggacaatttc ctgcaattga ttccttaaaa agtataagca
    27481 gggtatttac acaggttgtt gatgaaaaac atcgtattat ggccgctgca tttcgggagc
    27541 tactttctga aatagaagag ctaaggacaa ttattgactt tggtgaatac aaaccggggg
    27601 agaatgcctc tcaggataaa atctacaaca aaatatctgt tgttgagagt tttctgaagc
    27661 aagattatcg actcggtttt acttatgagc agacaatgga gcttattggt gaaacaatta
    27721 gataaggttt taaaaatcaa agataaatat caacggtctg ttaaattaat tgaagcacac
    27781 atattaacac taaaaaaaaa actctttgta tcgagatgtg gaggcattag ataaaaggat
    27841 tatatacttc ctgcaactgg agaatgactt agaacctgta ggcgctcaga gcgttagtca
    27901 gcttttcaat acaagacgta agatcgctat agtaaaaaag catattatcc aatatcagtc
    27961 tgaacgaatt cttctaaagg gaagaattga agaaattcaa aaagatattg atgaggcaaa
    28021 tgcttctaaa agaaaactgc ttcataaaga gagtaaaata tgtaaacgga ttggcttgat
    28081 taaaagaaat aactttgcca aacagttgat acttgatgag ttatcacaag aggatatgaa
    28141 gtatggcatt agacaatata aacctaaatt ttagtagtga caaacagata gaaaaatgtg
    28201 agaaactatc ttcaatagat aatattgata gtctcgtttt gaagaagaag aggaaggttg
    28261 aaattcctga gtactcttta attgcagcta actattttac tattgataag cactttgaac
    28321 ataagcatga taaaggagaa atttatagtg gcattaaaaa tgcgttcgaa cttagaaacg
    28381 aacgagcgac atattctgat attccagaat caatggccat taaagaaaat attttgatac
    28441 cagatcaaga tatcaaagca agggaaaaaa taaatatcgg cgatatgagg gggatctttt
    28501 catataataa gagtggaaat gcagacaaga acttcgaaag aagtcatact tcttctgtaa
    28561 accctgataa tctgctagaa tctgataata gaaatggtca aattggttta aaaaatcata
    28621 gcttgtctat tgataagaat attgctgaca tcatttcttt actaaatgga agtgttgcta
    28681 aatcatttga gctccctgta atgaataaaa atactgcaga cataacccca tccatgtcat
    28741 tgcaagaaaa atcaatagtt gaaaatgata aaaatgtttt tcaaaaaaat agtgaaatga
    28801 cttaccactt taaacagtgg ggggctggac attctgttag tatttcagtg gagtctggtt
    28861 cttttgttct aaaaccgtca gatcaatttg taggaaataa acttgactta attttgaaac
    28921 aagatgctga gggtaattac agatttgata gcagtcaaca taataagggg aataaaaata
    28981 atagtacagg atataatgaa cagagtgaag aagaatgcta agaattaaac attttgacgc
    29041 taacgaaaaa ctacagattt tatatgcaaa gcaactctgc gagcgttttt cgattcagac
    29101 attcaaaaat aaatttacag gcagtgaaag tttagtcact cttacttccg tgtgtgggga
    29161 ttgggtaatt cgtattgata cattatcttt tttgaaaaaa aaatacgagg tattttcagg
    29221 attttctaca caagaatctt tactgcattt atcaaaatgt gtctttatag agtcgtcatc
    29281 tgtattttcg attccagaac tgtctgataa gattactttc cggatcacga atgaaatcca
    29341 gtatgcaact actgggagtc atttatgctg tttttcctct tctttaggta ttatttattt
    29401 tgacaagatg ccggtattac gtaatcaagt ttctcttgac ttattgcatc atcttttaga
    29461 gttttgctta ggttcatcta atgtaaggct ggctacttta aaaagaattc gcactggtga
    29521 tataatcata gttcagaaac tttataattt attattgtgt aatcaagtta ttattgggga
    29581 ttatattgtg aatgataata atgaggcaaa aattaatctg tcagaaagta atggtgagtc
    29641 agaacacaca gaagtttctt tggcattatt caattatgat gatatcaatg taaaagtgga
    29701 ctttattctt ttagaaaaaa atatgacaat caatgaacta aaaatgtatg tagaaaacga
    29761 attattcaag tttcccgatg acatagttaa acatgtaaat attaaagtaa atggttcttt
    29821 ggttgggcat ggggaacttg tttctattga ggatggttat ggtatcgaga ttagttcttg
    29881 gatggtaaag gagtaatctc atgctgagtg acatgtccct catagctaca ctttcttttt
    29941 ttacgctatt accattttta gttgctgctg gtacctgtta tataaagttt tctattgttt
    30001 ttgtaatggt acgaaatgca cttggtttgc agcaggttcc atcaaatatg actctcaatg
    30061 gtattgcatt gattatggct ttgttcgtga tgaaacccat tattgaagct ggttatgaaa
    30121 attatctaaa tggccctcaa aaatttgata caatatctga tattgtccgt ttttctgatt
    30181 ctggcttgat ggaatataag caatacttga aaaagcatac agatcttgag ttagcacgtt
    30241 ttttccaaag gtctgaagaa gaaaatgctg atttaaagag tgcagagaat aatgattatt
    30301 cattgttttc cctcttgcct gcatatgcgc taagtgaaat caaagatgcg ttcaaaatag
    30361 gtttttattt atatttgcca tttgttgttg ttgatcttgt tatttcaagc attttacttg
    30421 cacttggtat gatgatgatg agtccaatca caatatcagt accaataaaa cttgtattat
    30481 ttgttgcgct tgatgggtgg ggtatcctgt ctaaggcctt gattgaacaa tatatcaata
    30541 ttcctgctta gatgatggag gtaatgtgtc tgatatagtt tatatgggta ataaggctct
    30601 ttatttaatc cttatctttt ccttatggcc agtaggtata gctacggtta ttggattatc
    30661 tattggtttg ttacagacag tgactcaact ccaagagcag acacttcctt ttggtataaa
    30721 gcttataggt gtctcaatat ctttgctact tctttctgga tggtatggtg aggttttatt
    30781 gtctttttgt catgaaataa tgtttttaat taagagtggg gtttgatgga catttcaagc
    30841 tggttcgaaa gtattcatgt gtttttaata ctcctgaacg gcgttttttt tagattggct
    30901 ccattatttt tctttcttcc atttttaaat aacggtataa tttctccatc tattagaata
    30961 cctgtgattt ttcttgttgc atcaggatta attacttctg gtaaggtaga cataggttct
    31021 tctgtttttg aacatgttta tttccttatg ttcaaggaaa taattgttgg cctccttctc
    31081 tctttttgct tgtctcttcc cttttggata tttcatgctg ttggtagcat tattgacaac
    31141 cagcgtgggg caacgcttag tagttcaatt gatcctgcca atggtgttga tacgtctgag
    31201 ttggcaaaat ttttcaatct tttttctgca gttgtatttc tatacagtgg tggtatggtc
    31261 tttattttag aatccataca attgtcttat aatatatgcc cgttattttc tcaatgttct
    31321 ttccgtgtct caaatatctt aacatttctg actttattgg caagtcaggc tgttatttta
    31381 gccagtcctg ttatgatagt attgttacta tcagaagtat tacttggtgt attatcgaga
    31441 tttgctccgc agatgaatgc tttttccgta tcattaacta ttaaaagttt acttgcaata
    31501 tttattattt tcatctgttc ttctactatt tacttttcta aagttcaatt tttcctcggt
    31561 gaacataaat ttttcacaaa tctatttgtt agataaaata ttatggcaaa taaaacagaa
    31621 aagccgacac ctaaaaaact aaaggatgcc gcaaaaaaag gacagtcatt taaatttaag
    31681 gatttaacga ctgttgttat tattctggta gggacattta ctataatatc attcttttcc
    31741 ttaagtgatg taatgctctt atacagatat gtaataatta atgacttcga aattaatgag
    31801 ggtaaatact tttttgcagt ggttattgtc ttttttaaga taattggctt cccacttttt
    31861 ttctgtgttc tttcggctgt gttgccaaca ttggttcaaa caaagtttgt tcttgcgact
    31921 aaagctatca agattgattt ttcagtatta aaccctgtta aagggttaaa aaaaatattt
    31981 agtataaaga caataaaaga atttttcaaa agcattctgc ttcttattat tctagcatta
    32041 acaacctatt tcttttggat taatgaccga aaaataattt tttctcaggt gttttctagt
    32101 gttgatggct tatatcttat ttgggggagg ctgtttaagg atataatatt atttttcttg
    32161 gcattttctg tttttgttat tattcttgac tttgtgattg agttcatttt atacatgaaa
    32221 gatatgatga tggataaaca ggagataaaa agagaatata tagagcaaga gggacacttt
    32281 gagacaaagt cgagaaggcg tgagttgcat atcgagattc tttcagagca gactaaatct
    32341 gatatacgta attcaaaatt agtggtaatg aacccgactc atattgcaat tggtatttat
    32401 tttaatccag aaatagcgcc tgcacctttt atttctctca ttgaaactaa ccagtgtgcc
    32461 ttggctgtca gaaaatatgc aaatgaagtt ggtataccga ctgtgcgtga tgtgaaatta
    32521 gctagaaaac tatataaaac acatacaaaa tatagttttg ttgattttga acacttggat
    32581 gaagtcctac gtcttattgt ttggcttgag caggttgaaa acactcatta ataagtaaag
    32641 gagatgtgtt atatgggagt taatttctgt aataaaatag gtattgatca gtctgaattt
    32701 gaaatagaat cttccatcat taactccatt gctaatgagg tattgaaccc aatatctttc
    32761 cttagcaata aggatataat aaatgtttta ctcagaaaga tttcatctga atgtgacctg
    32821 gttagaaaag acatttatcg ttgtgctctg gagttggtcg ttgaaaaaac acctgatgat
    32881 ttataatcat tcataataac tcttttgctg atataaatat ttttgttgaa gatatttatg
    32941 tagtcacagg atcaattgta gaaaattgtt atgtttttgt taggaactgc actaaataat
    33001 atatgataag acagcaaaaa cgactgacca taatccttct tttattagga gttgataaga
    33061 gagactattc atcctgtaat gttaaaactc tgttatatag tattcgcgat tatgcaaagt
    33121 ctgtaaatga tcatgagata ctaaccgaga gtaatcgttt attatctcat tgcatttcag
    33181 atagtaacgg agctttcttt aaaagtagta aatacgttcc ccttaagtac ttacgtaaaa
    33241 ggagaatagc acgaaagata ccaaatgatt agcactttgc tagtaagctt gctggtaaac
    33301 tatattgacc gttgggtttg ttttgagact gtaatttaaa ttgtgtaatt gcctgttttt
    33361 gatatgttta ctccaacaac ggagacaggt aaa
//
LOCUS       DADLTF010000027        33271 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.027, whole genome shotgun sequence.
ACCESSION   DADLTF010000027 DADLTF010000000
VERSION     DADLTF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.027"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="JN749_001239"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="JN749_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HAY7367716.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="JN749_001240"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="JN749_001240"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAY7367717.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JN749_001241"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="JN749_001241"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HAY7367718.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JN749_001242"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="JN749_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAY7367719.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JN749_001243"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="JN749_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAY7367720.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /locus_tag="JN749_001244"
                     /pseudo
     CDS             complement(3421..4089)
                     /locus_tag="JN749_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="JN749_001245"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="JN749_001245"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HAY7367721.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="JN749_001246"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="JN749_001246"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAY7367722.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="JN749_001247"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="JN749_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HAY7367723.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JN749_001248"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="JN749_001248"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAY7367724.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JN749_001249"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="JN749_001249"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAY7367725.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="JN749_001250"
     CDS             8207..9070
                     /locus_tag="JN749_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAY7367726.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="JN749_001251"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="JN749_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HAY7367727.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="JN749_001252"
     CDS             10406..11023
                     /locus_tag="JN749_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HAY7367728.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="JN749_001253"
     CDS             complement(11020..12537)
                     /gene="ligB"
                     /locus_tag="JN749_001253"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HAY7367729.1"
                     /translation="MEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTG
                     VRKMADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQK
                     VSLISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTL
                     NSLAVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLP
                     FVTDGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVA
                     SLAPVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLGGQGIPRIDDVVWRGAERTK
                     PTPPENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEH
                     IFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASD
                     ERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(12559..12816)
                     /locus_tag="JN749_001254"
     CDS             complement(12559..12816)
                     /locus_tag="JN749_001254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367730.1"
                     /translation="MIYCNKDDSQEASSQTPFLPLGFAFTTLYICQPDYSGGEDESMD
                     GDINKYLVLAIICVGGLSGLVASQSTGRNFPPATANKTVGR"
     gene            12956..13579
                     /gene="gmk"
                     /locus_tag="JN749_001255"
     CDS             12956..13579
                     /gene="gmk"
                     /locus_tag="JN749_001255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAY7367731.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13634..13909
                     /gene="rpoZ"
                     /locus_tag="JN749_001256"
     CDS             13634..13909
                     /gene="rpoZ"
                     /locus_tag="JN749_001256"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAY7367732.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13928..16036
                     /gene="spoT"
                     /locus_tag="JN749_001257"
     CDS             13928..16036
                     /gene="spoT"
                     /locus_tag="JN749_001257"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HAY7367733.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16043..16732
                     /gene="trmH"
                     /locus_tag="JN749_001258"
     CDS             16043..16732
                     /gene="trmH"
                     /locus_tag="JN749_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HAY7367734.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16738..18819
                     /gene="recG"
                     /locus_tag="JN749_001259"
     CDS             16738..18819
                     /gene="recG"
                     /locus_tag="JN749_001259"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAY7367735.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="JN749_001260"
     CDS             complement(18853..20058)
                     /gene="gltS"
                     /locus_tag="JN749_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HAY7367736.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20338..21729
                     /gene="xanP"
                     /locus_tag="JN749_001261"
     CDS             20338..21729
                     /gene="xanP"
                     /locus_tag="JN749_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HAY7367737.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21850..23559
                     /locus_tag="JN749_001262"
     CDS             21850..23559
                     /locus_tag="JN749_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7367738.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="JN749_001263"
     CDS             complement(23612..25930)
                     /gene="yicI"
                     /locus_tag="JN749_001263"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HAY7367739.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNDQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25940..27323)
                     /locus_tag="JN749_001264"
                     /pseudo
     CDS             complement(25940..27323)
                     /locus_tag="JN749_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27616..27710
                     /locus_tag="JN749_001265"
     tRNA            27616..27710
                     /locus_tag="JN749_001265"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27650..27652,aa:Sec,seq:tca)
     gene            27982..29194
                     /locus_tag="JN749_001266"
                     /pseudo
     CDS             27982..29194
                     /locus_tag="JN749_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            29606..30649
                     /locus_tag="JN749_001267"
     CDS             29606..30649
                     /locus_tag="JN749_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAY7367740.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSRIQYQ"
     gene            31164..31625
                     /locus_tag="JN749_001268"
     CDS             31164..31625
                     /locus_tag="JN749_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367741.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTDPTHVMWTRP"
     gene            complement(31622..32477)
                     /locus_tag="JN749_001269"
                     /pseudo
     CDS             complement(31622..32477)
                     /locus_tag="JN749_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(32474..32773)
                     /locus_tag="JN749_001270"
     CDS             complement(32474..32773)
                     /locus_tag="JN749_001270"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7367742.1"
                     /translation="MTKPVSISKKPRKQHTPEFRNEALKLAERIGVAAAARELSLYES
                     QLYAWRSKQQQQMSSSERESELAAENVRLKRQLAEQAEELSILQKAATYFAKRLK"
BASE COUNT         8326 a         8751 c         8211 g         7983 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccagaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacacgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gcagctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaacct gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt ttttttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gccccagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccgccaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 attggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cactacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccagtagtc atcgtcccac tgttttattt gctgttgcag gcgggaaatt
    12601 tcttcctgtg ctctggctgg cgaccaggcc ggacagaccg cccacacaga tgattgccag
    12661 cacaagatac ttattaatat cgccatccat actttcatct tcacctccgc tgtagtcagg
    12721 ctggcagata tacaacgtgg tgaaggcaaa gccgagtggc aaaaacggag tctgcgagga
    12781 cgcttcctga gaatcgtctt tattgcagta aatcacaggc aaatgcggaa acagctacgc
    12841 aaaacgcaac aactttgcgc aaaaagtgtg agcaagggct acgtcacatg gccgcgccgt
    12901 gtataataag ctcgtatgta ggctttattt cgctaatcac atacgaaaga tactcatggc
    12961 tcaaggcacg ctttatattg tttctgcccc cagtggcgcg ggtaaatcca gcctgattca
    13021 ggctttatta aaaacccaac cgttgtatga cacccaggtt tctgtttcac acaccacacg
    13081 ccaaccgcgt cctggtgaag tccacggtga acattatttc tttgttaatc atgatgaatt
    13141 taaagaaatg attagcagag atgcgttcct cgaacacgca gaagtttttg gtaattacta
    13201 tggcacttcg cgtgaggcca ttgagcaagt actggcgacc ggtgtcgatg tttttctcga
    13261 tatcgactgg cagggcgcgc agcaaattcg ccagaagatg ccgcacgcgc ggagtatctt
    13321 tattttaccg ccgtccaaaa ttgaactgga ccgccgtcta cgcggtcgcg gtcaggacag
    13381 cgaagaggtc attgcaaagc gtatggcgca agctgttgca gaaatgagcc attacgccga
    13441 atatgattat ctgattgtga atgatgactt cgataccgcg ttgaccgatt tgaagaccat
    13501 tattcgcgcc gaacgtctgc gcatgagccg ccaaaagcag cgtcatgacg ctttaatcag
    13561 caaattgttg gcagactgaa cctgatttca gtatcatgcc cagtcatttc ttcacctgtg
    13621 gagcttttta agtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg
    13681 ttttgacctg gtactggtcg ccgcgcgtcg cgctcgtcag atgcaggtag gcggaaagga
    13741 tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga
    13801 aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga
    13861 agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat cacaaagcgg
    13921 gtcgcccttg tatctgtttg aaagcctgaa tcaactgatt caaacctacc tgccggaaga
    13981 ccaaatcaag cgtctgcggc aggcgtatct cgttgcacgt gatgctcacg aggggcaaac
    14041 acgttcaagc ggtgaaccct atatcacgca cccggtagcg gttgcctgca ttctggccga
    14101 gatgaaactc gactatgaaa cgctgatggc ggcgctgctg catgacgtga ttgaagatac
    14161 tcccgccacc taccaggata tggaacagct ttttggtaaa agcgtcgccg agctggtaga
    14221 gggggtgtcg aaacttgata aactcaagtt ccgcgataag aaagaggcgc aggccgaaaa
    14281 ctttcgcaag atgattatgg cgatggtgca ggatatccgc gtcatcctca tcaaacttgc
    14341 cgaccgtacc cacaacatgc gcacgctggg ctcacttcgc ccggacaaac gtcgccgcat
    14401 cgcccgtgaa actctcgaaa tttacagccc gctggcgcac cgtttaggta tccaccacat
    14461 taaaaccgaa ctcgaagagc tgggttttga ggcgctgtat cccaaccgtt atcgcgtaat
    14521 caaagaagtg gtgaaagccg cgcgcggcaa ccgtaaagag atgatccaaa aaatcctctc
    14581 ggaaatcgaa gggcgtttgc aggaagcggg aattccctgc cgcgtcagtg gtcgcgagaa
    14641 gcatctttat tcgatttact gcaaaatggt gctcaaagag cagcgttttc actcaatcat
    14701 ggacatctac gctttccgcg tgatcgtcaa tgattccgac acctgttatc gcgtgctcgg
    14761 gcaaatgcat agcctgtaca agccgcgtcc gggtcgcgtg aaagactaca tcgccattcc
    14821 aaaagcgaac ggctatcagt ctttgcacac ctcgatgatc ggcccgcacg gtgtgccggt
    14881 tgaggtccag atccgtaccg aagatatgga tcagatggcg gagatgggtg ttgccgcgca
    14941 ctgggcttat aaagagcacg gcgaaaccag caccaccgcg caaatccgcg cccagcgctg
    15001 gatgcaaagc ctgctggagc tgcaacagag cgccggtagt tcgtttgaat ttatcgagag
    15061 cgttaaatcc gatctcttcc cggatgagat ttacgttttc acaccggaag ggcgaattgt
    15121 cgagctgcct gccggtgcaa cgcccgtcga cttcgcttat gcagtgcata ccgatatcgg
    15181 tcatgcctgc gtgggcgcac gcgttgaccg ccagccttac ccgctgtcgc agccgcttac
    15241 cagcggtcaa accgttgaaa tcattaccgc accgggcgct cgcccgaatg ccgcgtggct
    15301 gaactttgtc gttagctcaa aagcgcgcgc caaaattcgt cagttgctga aaaacctcaa
    15361 gcgtgatgat tctgtaagcc tgggccgtcg tctgctcaac catgctttgg gtggtagccg
    15421 taagctgaat gaaatcccgc aggaaaatat tcagcgcgag ctggatcgca tgaagctggc
    15481 aacgcttgac gatctgctgg cagaaatcgg acttggtaac gcaatgagcg tggtggtcgc
    15541 gaaaaatctg caacatgggg acgcctccat tccaccggca acccaaagcc acggacatct
    15601 gcccattaaa ggtgccgatg gcgtgctgat cacctttgcg aaatgctgcc gccctattcc
    15661 tggcgacccg attatcgccc acgtcagccc cggtaaaggt ctggtgatcc accatgaatc
    15721 ctgccgtaat atccgtggct accagaaaga gccagagaag tttatggctg tggaatggga
    15781 taaagagacg gcgcaggagt tcatcaccga aatcaaggtg gagatgttca atcatcaggg
    15841 cgcgctggca aacctgacgg cggcaattaa caccacgact tcgaatattc aaagtttgaa
    15901 tacggaagag aaagatggtc gcgtctacag cgcctttatt cgtctgaccg ctcgtgaccg
    15961 tgtgcatctg gcgaatatca tgcgcaaaat ccgcgtgatg ccagacgtga ttaaagtcac
    16021 ccgaaaccga aattaatgtt ttatgaaccc aacacgttat gcacgcatct gcgaaatgct
    16081 cgccaggcgg cagcctgatc tgaccgtctg catggagcag gtccacaaac ctcataacgt
    16141 ttctgcgatt attcgtaccg cagatgccgt tggcgtacat gaagttcacg ccgtctggcc
    16201 tggtagccgc atgcgcacca tggcttcggc agcggcgggt agtaacagct gggtacaggt
    16261 gaaaacacac cgcaccattg gcgatgccgt cgctcatctc aaaggccagg gcatgcagat
    16321 tctggcaacc catctttctg ataatgctgt cgatttccgc gaaattgatt acactcgccc
    16381 gacctgcatt ttgatgggac aggagaaaac gggcatcacg caggaagcat tggccctggc
    16441 ggatcaggac atcatcattc cgatgatcgg catggtgcag tcgctgaatg tttccgttgc
    16501 ctcagccctc attctttacg aagcccagcg tcagcggcaa aatgcaggca tgtacctgcg
    16561 tgaaaacagc atgttgccgg aagcagagca acaacgcctg ttgtttgaag gcggctatcc
    16621 ggtgctggcg aaagtcgcaa aacgcaaagg cctgccttat ccccacgtca atcagcaagg
    16681 cgagatcgaa gctgatgccg actggtgggc tactatgcag gctgcagggt aagtgccatg
    16741 aaaggtcgcc tgttagatgc tgtcccactc agttccctaa cgggcgttgg cgcagcactt
    16801 agtaacaaac tggcgaaaat caacctgcat accgtgcagg atctactctt acaccttccc
    16861 ctgcgctacg aagatcgcac ccatctctac cccatcggag aactactgcc gggcgtttat
    16921 gccacggtgg aaggcgaagt gctgaactgc aatatctcct tcggcggtcg gcggatgatg
    16981 acctgccaga tcagcgacgg ttccggcatc ctcaccatgc gctttttcaa tttcagcgcg
    17041 gcaatgaaaa atagcctggc gacgggccgc cgtgtactgg cttatggcga agcaaagcgc
    17101 ggtaaatatg gtgcggagat gatccacccg gaataccgcg tgcagggcga tctcagcacg
    17161 ccagaattac aggaaacgct cacgccggtt tatccaacaa cggaaggcgt aaagcaggcc
    17221 acgctgcgta aattaaccga ccaggcgctg gatctgctcg acacctgcgc cattgaagaa
    17281 ctcctgccgc cggaactgtc acaaggaatg atgacgctac cggaagcgtt gcgcactttg
    17341 caccgcccgc caccgacgct acagcttagc gatctggaaa ccgggcagca tccggcgcaa
    17401 cgtcgtctga ttctggaaga actgctggcg cacaacctca gcatgttagc cttacgtgcc
    17461 ggagcacagc gttttcatgc ccagccgctg agcgccaatg acacgctgaa aaataaactc
    17521 ctcgccgcct taccgttcaa gccaacgggc gcacaggcac gcgtagtggc ggagatcgag
    17581 catgatatgg cgctggatgt gccgatgatg cgcctggtac agggcgatgt aggttccggt
    17641 aaaacgctgg tcgccgccct cgccgcgctg cgtgcgattg cccacggcaa acaggtggca
    17701 ctgatggcac caaccgaatt actcgccgag cagcacgcca ataacttccg caactggttt
    17761 gcaccgctcg gtatcgaagt gggctggctc gccggtaagc agaaaggcaa agcgcgactg
    17821 tctcagcagg aagccatcgc cagcggtcag gtgcagatga ttgtcggtac acacgccatc
    17881 ttccaggaac aggtgcagtt taacggcctg gcgctggtga ttatcgacga acagcatcgt
    17941 tttggcgtgc atcagcgtct ggcattgtgg gagaaaggcc agcagcaggg cttccatccg
    18001 catcagttga tcatgaccgc cacgccgatc ccccgcacgc tggcaatgac ggcgtatgcc
    18061 gatctcgata cctcggtgat agatgagctg ccgccaggcc gcacgccagt gactacggtc
    18121 gctattcctg atacccgccg taccgacatc attgaccgcg tgcgccacgc ctgcataact
    18181 gaaggtcgtc aggcgtactg ggtttgtacg ttgattgaag agtcagagtt actggaagcg
    18241 caggcggcgg aagctacctg ggaagagttg aaactggcgc taccagaatt gaatgttggc
    18301 ctggtacacg ggcggatgaa accagccgag aaacaggcgg tgatggcgtc gtttaaacaa
    18361 ggtgagctac atctgctggt tgccactacc gttattgaag tcggtgttga tgtgcctaac
    18421 gccagcctga tgattatcga aaaccctgag cgtctgggtc tggcgcagtt acaccagctg
    18481 cgtgggcgcg taggtcgtgg cgcggtggct tctcactgcg tgctgctcta caaaacgccg
    18541 ctttctaaaa cggcgcaaat tcgcctgcaa gtgctgcgcg acagtaacga cggttttgtg
    18601 attgcgcaaa aagatctgga gattcgcggc cctggcgaat tgttaggcac gcgtcagacg
    18661 ggtaatgctg aatttaaagt ggcggattta ctgcgcgatc aggcgatgat cccggaagtt
    18721 cagcgcctgg cgcgacatat tcacgaacgt tacccacaac aggcaaaagc cttgatagaa
    18781 cgctggatgc cggagacaga acgttactcg aatgcgtaaa aggcggcggt gagaagaccg
    18841 ccgcttcatc ggttaaccgg caaaaatcgg caacattaaa tacagcttaa tcaccagcgc
    18901 attgacgata tcgataaaga acgcaccgac catcggcacc accaaaaacg ccatgtgcga
    18961 cgggccaaag cgttcagtga tcgcctgcat gttggcgatt gccgttggcg ttgcaccgag
    19021 gccaaaacca cagtgacccg cagccagcac tgccgcatcg tagtttttgc ccatcatgcg
    19081 ccaggtaacg aagatggcat acaacgccat gaagatggtc tgtaccacca gaatcgccag
    19141 catcggcagt gccagcgaag ccagctccca cagtttcagc cccatcaacg ccatcgccag
    19201 gaacaacgac aagcttacgt tacccagcac ggataccgca cgctcaaaga cgcggtaaaa
    19261 gcccattatt gacagaccgt tgctcagaat cacgccaaca aacagtacac agacgaaggt
    19321 cggcagttca aaagcagtgc cagccaaaag ttgcgcaaca attttcccca ccgtcaggca
    19381 gatagcaatc agcgcgatag tttcaatcag caccaacgag gtgatcatgc gtcccacatc
    19441 cggcttttca aacgccgtcg ggacttcctg gtcatccgga ataccgttcg gcgtggtgga
    19501 gtgtttcacc agatagcgcg ccaccggacc gccaatcaag ccgcccagca ccagaccgaa
    19561 cgttgcacag gccatagcca cttccgtcgc attggtgaag ccataacgtt caatgaacaa
    19621 tttactccac gcagcgcccg taccgtgacc gccggaaagc gtaatagaac cggccaacag
    19681 ccccatcagc ggatcaagcc ctaacaggct agccatacca atgccaatgg cattttgcat
    19741 caccaacaga ccaacaacca caatcaagaa gatgccaacc acacgcccac cggcacgcaa
    19801 actggcaatg ttggcgttca ggccaatggt ggcgaagaaa gccagcatta acggatcgcg
    19861 cagggacata tcaaagttga cttcccagcc catgcttttt ttcagtacta gtagcgccag
    19921 cgccaccaac aaaccacccg caacaggttc cggtatggtg tatttcttca aaaaggagac
    19981 ggaatggacc aacttacgcc cgagcagcaa cgtcagcgtt gcggcaacaa gcgttgctaa
    20041 agtatcgaga tgaaacatag ttactccttt gtatccgcat cactctttca tactcgtcat
    20101 acttcatcgc gcagtggcaa cttcatgaac tatgacgggt atcgagttat gctttcctgg
    20161 gtcaaattct cagctgttaa tgaacaaatg gcatggattt taagcagaaa aataccaaaa
    20221 gttatataaa aaagtgcatt tatctcattt aaatatttag atgaggatgt ggcaaacgtt
    20281 tgcttttctc tttcggtcag ctaaaatgcc cgctttgcta ccacgggatt gttttcaatg
    20341 tctgtttcca ccctcgagtc agaaaatgcg caaccggttg cgcagactca aaacagcgaa
    20401 ctgatttacc gtcttgaaga tcgtccgccg cttcctcaaa ccctgtttgc cgcctgtcag
    20461 catctgctgg cgatgttcgt tgcggtgatc acgccagcgc tattaatctg ccaggcgctg
    20521 ggtttaccgg cacaagacac gcaacacatt attagtatgt cgctgtttgc ctccggtgtg
    20581 gcatcgatta ttcaaattaa ggcctggggt ccggttggct ccgggctgtt gtctattcag
    20641 ggcaccagct tcaactttgt tgccccgctg attatgggcg gtaccgcgct gaaaaccggt
    20701 ggtgctgatg ttcctaccat gatggcggct ttgttcggca cgttgatgct ggcaagttgc
    20761 accgagatgg tgatctcccg cgttctgcat ctggcgcgcc gcattattac gccgctggtt
    20821 tctggcgttg tggtgatgat tatcggcctg tcgctaattc aggtggggct gacctccatt
    20881 ggcggcggtt acgcagccat gagcgataac accttcggcg caccgaaaaa tctgctgctg
    20941 gcaggcgtgg ttttagcctt aattatcctg cttaaccgtc aacgtaaccc ttacttacgc
    21001 gtggcctcac tggtaattgc gatggcggcc ggatatgcgc tggcgtggtt tatgggcatg
    21061 ttgccagaaa gcaacgaacc gatgacgcaa gaactgatta tggtgccaac gccgctctat
    21121 tacggtcttg gcattgaatg gagtctgctg ctgccgctga tgctggtctt tatgatcact
    21181 tcgctggaaa ccattggcga tatcacggcg acctctgacg tttccgaaca gccagtgtcc
    21241 ggtccgctgt acatgaaacg cctgaaaggc ggcgtgctgg caaacggcct gaactcgttt
    21301 gtttcggcgg tgtttaacac cttcccgaac tcctgcttcg ggcaaaacaa cggagtgatc
    21361 cagttgactg gtgttgccag ccgctatgtc ggttttgtcg tcgcgctgat gttgatcgtg
    21421 ctgggtctgt tcccggcagt gagcgatttt gtacaacaca ttccagaacc ggttctgggc
    21481 ggcgcaacgc ttgtaatgtt tggcaccatc gccgcctccg gtgtgcgtat cgtttctcgt
    21541 gagccgctga accgtcgggc gattctgatt atcgcgttgt cgctggcggt tggtctgggc
    21601 gtgtctcagc agccgctgat tttgcagttt gcccctgaat ggctgaaaaa cctgctctcc
    21661 tccgggatcg ccgcgggcgg tattactgcc atcgtgctga atctgatttt cccaccagaa
    21721 aaacagtaat tcactggcgg cggtaagata attaccgccg cagtctcacc tctttcacca
    21781 ttcccgcctt gaggattgcg cgtaaatcgt gcataactcc cttatgtgca tttcacggga
    21841 tggaagacca tgaaatttat tgggaagctg cttctctaca ttctcatcgc tctgttagtg
    21901 gtgatcgctg gcctctattt tcttctgcaa acccgctggg gagcagaaca tatcagcgca
    21961 tgggtttccg agaatagcga ctatcatctg gccttcgggg cgatggatca ccgtttttcc
    22021 gcgccatctc atatcgtgct ggagaatgtc acgtttggtc gtgatggtca gcccgcgccc
    22081 ctggtggcaa aaagtgtcga cattgcgcta agcagtcgcc aactgaccga accacgccat
    22141 gtcgatacca tcctgctgga aaacgggacg ctgaatctca ccgaccagac cgcgccgcta
    22201 ccgttcaaag ccgatcgtct gcaactgcgt gatatggcgt ttaatagccc gaatagcgaa
    22261 tggaaactga gcgcgcagcg ggtaaatggc ggcgtggttc cgtggtcacc agaagccggt
    22321 aaagtgctgg gtacgaaggc gcagattcag tttagtgccg gatcgctttc gctcaatgat
    22381 gttcctgcca ccaatgtact gattgaaggc agtattgata acgatcgcgt tacgctgact
    22441 aacctgggtg ccgacatcgc ccgcgggaca ttaaccggaa acgcgcagcg taacgccgac
    22501 ggcagctggc aagtggaaaa tctgcgcatg gcggatatcc gtctacaaag cgaaaaatcg
    22561 ctaaccgact tctttgcgcc attacgctct gtcccgtcgt tgcagattgg tcgcttggaa
    22621 gtcatcgatg ctcgtctgca aggtccggac tgggcggtga ccgacctcga tctcagcttg
    22681 cgcaacatga ccttcagtaa agatgactgg cagacacagg aaggcaaact gtcgatgaac
    22741 gccagcgagt tcatttatgg ttcgctgcat ttatttgacc cgattataaa cgcggaattt
    22801 tccccgcagg gcgtagcgct gcgccagttc accagccgct gggaaggggg tatggtcaga
    22861 acgtcaggga actggctgcg tgacggtaaa acgttgatcc ttgatgatgc ggcaattgcc
    22921 gggctggaat ataccttgcc gaaaaactgg caacagttgt ggatggaaac gacacccggt
    22981 tggttaaaca gcctgcaact gaagagattt agcgccagcc gcaatctgat cattgatatc
    23041 gaccctgact tcccgtggca gctcaccgcg ctcgatggtt acggtgccaa cctgacgctg
    23101 gttaccgatc ataaatgggg cgtctggagt ggctcggcga atctgaatgc cgccgccgcg
    23161 acattcaatc gtgttgatgt tcgtcgcccg tcgctggcgc tgaccgccaa cagcagcacg
    23221 gtgaatatca gcgaactgag tgcatttact gaaaaaggca ttctggaagc caccgcaagt
    23281 gtttcacaaa cgccacaacg tcagacccat atcagcctga atggacgcgg tgtgccggtg
    23341 aatattttgc aacaatgggg atggcctgaa ttacagttga ctggcgacgg caatattcag
    23401 cttaccgcca gtggagatat tcaggccaat gccccgctga aacctacggt tagcgggcaa
    23461 ttgcatgccg tgaacgccgc aaagcagcaa gtgactcaaa ccatgaatgc gggcgtcgtt
    23521 tccagcggtg aagttacatc gacggagccg gtgcggtaaa catcaatctc gccgggtacg
    23581 actcagacgt acccggcatt ccgtcaatag attacaacgt aattgtcagc gcattccctt
    23641 gaggcgtcac caccagcccc tgctcacttt cagcctgcga accgccttgc agaccattta
    23701 ctttcacaac attgcgcaag cacagcgtcc agttcttcgc ctcgcccgca ccagtcacag
    23761 taatcgtgtt gccagtacgt gctgctttta aagtaaagat caccgatccg tcagcagcag
    23821 gcacttcaca gacggcttca tgcccgtctt gcagattaaa gaggtggaat gccgtgcctt
    23881 cgtgccacac gtaatcggga cgttgatcgt tgttgcccag cgccagtaga gtgttatcac
    23941 gcacataaac gggcagactc aggaagccgt gctgctgttt atgccagcga ctaccgtcga
    24001 gttcatcgtt gtgccacagg tgtgtccagc gaccttccgg caggtagaac tgcacatcgc
    24061 ccgcttcagt gaacaccggc gcaaccatca cgttgtcgcc taacatgtat tgacggtcaa
    24121 ggtaatcaca agccggatcg tccgggaact ccatcatcat ggcccgcatc atcggcgtac
    24181 cccgcgcgtt cgcacgcgca gcttcacgat acagatacgg catcatgcgg catttcagtt
    24241 gcgtgaagaa gcgcaccaca tcacaggact catcatcgta ggcccacggc acacgataag
    24301 atttgctacc gtgtaaacgg ctatggctgg agagcaaacc aaacgcgcac cagcgtttgt
    24361 aaacgtgcgc cggagcggta ttttcaaagc cgccgatatc gtggctccag aagccaaaac
    24421 ctgaaaggcc aatagacaaa ccaccgcgca ggctttccgc cattgattcg tagttagcgt
    24481 aacaatcgcc accccagtgt accgggaatt tctgcgcacc gacggaggcc gagcgggcaa
    24541 acaagacagc ttcttcctca ccaacggtgt ccttgagcac gttccacacc agttcgttgt
    24601 agatgtacgc ataatggtta tgcattttct gcggatcgga accgtcaaac cactgaacat
    24661 cagttgggat acgttcgcca aagtcggtct taaagcaatc aacgcccatc gcgaccagac
    24721 ctttcagttt gtcggcgtac catttgcagg catccggatt ggtaaagtca taaatcgcca
    24781 gacctggctg ccatttatcc cactgccata gcgaaccgtc cgggcgtttg agtaaatagc
    24841 ctttctcttg taactcttta aagacggggg atttttgacc gatatagggg ttaatccaga
    24901 cgcagatttt cagccctttc gctttcaggc ggcggatcat cccttccggg tcagggaaag
    24961 tcagcgggtc ccactcaaaa tcgcaccact ggaaggcttt catccagaaa cagtcaaagt
    25021 ggaaaacatg cagcggcaga ttgcgttccg ccataccatc gataaagctg tttaccgtcg
    25081 cttcgtcgta gttggtggta aatgaagtgg ttagccacag gccgaaggac cacgggggcg
    25141 gcagcgccgg acgaccagta aagcgggtat aacgatcaag taccgctttc ggcgtcgggc
    25201 cgtcgataac aaagtattcg agatattcac tctcaacgct gaactgcact ttggagactt
    25261 tctccgatcc cacttcaaaa gagacacact ggggatgatt gaccagtacc ccataaccac
    25321 ggttagtcat gtagaacggg atatttttat acgcctgttc agtacttgtg ccgccgtccc
    25381 ggttccaggt ctctaccgtc tggtcattgc gcaccagggc agtaaagcgc tctcccagac
    25441 cgtaaactgt ttcgccaacg ccaagatcaa gccgctcaaa catataattg cgttgattat
    25501 tcgtgtcctg cacgtagcca ttatttttca cctgactacc ggtaatacgt tcgccgttgc
    25561 gcagaaaatc cagtgaccag aactcacctt tgctgacacg cgcgcttaag ttgccacttt
    25621 taaactcagc ataacgttct gtgttttcga ttgtgacctt cacgtcctgc aaaatattga
    25681 gcggataatg aggaccgtta ttcagcgccc cctgaaaata ctcaatccgc acaccgacaa
    25741 taccttcctg tggggagaaa aagcgcagcg taaataaagg cgtatcaagc tgccaggtac
    25801 gttcacgcac atcacggggg gcagcataga ccaccatttc attatcctgc tgttcaacct
    25861 cgaatacctg aagcgggtga atcaaattga gcccaggttg aatcaaccag tttccatcgc
    25921 taattttcat tgccgttcct tagttctgca cttcttgaga ggtgaattgt tgctggcaac
    25981 ggcgtttacc ctgagccagc tgttccataa cggttttcag attgtgcgtc gtgagtgagt
    26041 agtagcgttt agcgataatc gcgctcagca aataacagat cgccggaaca atcgtgaata
    26101 gcgcaataat gatgctaatc gtggcgctgt tctgcgcttt ttctgccgca tcatatccgc
    26161 cataagccag catccagccg ataagagcgc cgccgaaggc caaccccagt ttgagcacaa
    26221 acagcgtgcc agcaaaactg atcccggtca ggcgtttacc attgcaccac tcgccgtagt
    26281 cgacggtatc gaacatcatt acccactgga taggtgtcac cagttgatgc aacacaccaa
    26341 tcacgaagat gaagacaaac atagtgatgc tggcctgcat gggaacaaag aacatcgcga
    26401 ggctaatcac tgccagcagg gcgttcgtcc accagaagat agtgacttta catttccagt
    26461 cggtcagagg ttttgccagt gcggaaccaa tcaggttacc cacgcaataa gtggtgagaa
    26521 aagcgacaaa cacttccggc gtgcccaaaa tccatgtgac gtaatacatc atcgccccac
    26581 cgcgtacgca caccgccagg atattgaaaa tggttagtaa accgacaatc cgccactggt
    26641 cgttttgcca gatatcacgt aaatcttccc gcatagacgt tgttgtaggt ggtgcttcaa
    26701 cgcgttcttt agtggtgaag aaacaaaatg ccagcatcat taatgccacc acggaaagga
    26761 ccacgatacc gccctggaaa ccgagtggtt tattatcacc gccaattaaa ttaaccagtg
    26821 gcatcatcag aacagtagaa agcatgcctc ccgcggtcgc cagcacaaaa cgccaggatt
    26881 gcagcgagat acgctgagtc gggtcattgg tgattacacc acccaatgcg cagtaaggga
    26941 tattgacgac ggtataaagt aaggtaagtt agggtgtaag taattgctgc atagatcatt
    27001 ttgccgttca tactgagatc tggcgtgcta taggccagta cacagacgat cccgaatggc
    27061 agtgcgccaa acagtaccca cggacgaaat ttaccccagc gagagcgcgt tcgatcggcc
    27121 aacaacccca tgcaaggatc ggaaatcgca tccagtgcac gagcgaccaa aaacatggtt
    27181 ccgacaaatc cggcaggaat gccaaaaata tcggtataaa agaacatcat atataacatt
    27241 acgttatcga aaataatgtg gctggcggcg tctcccatgc cataaccaat tttctcttta
    27301 acggacaaca cttcactctt catctgcttt tccttgatgc tataggggtg ctgagaccga
    27361 taacgcattt tgtaaaccat ccctgtgaga gcgaatattc cgatatctgg ttattaattt
    27421 atgattcttg ttttatgtga tcgtggtagc gttaattccg ctcatatatc attgtaaaat
    27481 atgggtttta tataaattat aatgctttcg tgataatacg ctgcgtttat taggcggaaa
    27541 aaactgatct gggggatgta gaaactcaag gaagtagcta taatgcgccc cgcctccatg
    27601 tagcaatcga ggcgcggaag atcgtcgtct ccggtgaggc ggctggactt caaatccagt
    27661 tggggccgcc agcggtcccg ggcaggttcg actcctgtga tcttccgcca gtttacctct
    27721 tctgaactct cccaatgtca ataaatctct tctaatccct tgtatatcag tatttatttg
    27781 tatcctgagg tcaacctact tcaccccgta tcaagtttct tttgggggta ctttaggggg
    27841 tgttatttcc tattgaaata caagcacccc cataatagga aaaatgtgat acataacaac
    27901 ggcttttaac aatatctttg ttatacaagg ggtgtattgg gggtatcagg ttagttactg
    27961 gcagaaatcc ccctcaggaa catgaattca tcaaaagccg gaggatgtca tggcattaac
    28021 cgatatcaaa gtgaaaaccg caaaacccaa agacaaaccc tataagcttg ctgatggcgg
    28081 cggtatgtat ctactgatta acactaatgg ttctaaatac tggcgcatga agtaccgctt
    28141 tgctggtaaa gaaaagatgc tttctattgg cgtatacccc gatgtaacgc tagccgatgc
    28201 tcgcgagaaa cgcagtgaag ccaggaaact cctcgctgct ggaggcgatc caggggaggc
    28261 aaaaaaggaa gaaaaaatcg cgcagcaaat gagtctgaaa aatacgttcg aggctattgc
    28321 tcgcgaatgg caccagttaa aagctgatcg gtggtcatta cgctatcgtg atgaaatcat
    28381 tgataccttt gaaaaagaca tttttcccta cattggcaag cgccctattg ccgaaatcaa
    28441 accaatggag ttactggaag cactgcgtaa aatggaaaaa cgtggagctc tggagaaaat
    28501 gcgcaaggtc cgccagcgct gtggtgaggt gttccgctat gcaattgtta ctggccgggc
    28561 agactataac ccagcccccg atcttgccag tgctttagct acaccgaaaa aagtacattt
    28621 tccctttctt actgctaatg aacttcccca cttcctcaac gatctggcgg gttataccgg
    28681 aagtataatc actaagacag ctactcagat cattatgtta accggcgtac gaacacaaga
    28741 actgcgtttt gcacgctggg aagatatcga ttttgagaca aaattgtggg agatcccggc
    28801 agaagtaatg aagatgaagc gacctcatat tgtgccgctc tctgagcagg tcattatgct
    28861 atttaagcag cttgaaccaa tctcaaaaca tcatccccta gtctttatcg gtagaaatga
    28921 tccccgcaaa ccaattagta aggaaagcat taaccaagta attgaattac ttggatataa
    28981 gggaagactc acagggcacg gtttcagaca caccatgagt acaattctgc atgaacaagg
    29041 ctttaattcc gccttgattg aaatgcagtt agctcatgtg gataaaaatt ccatcagggg
    29101 tacctataat catgccctgt atctcgatgg ccgccgtgaa atgatgcaat ggtacgctga
    29161 ttacattgat tcactctcca gccgggaaag ttaagtcaga aatacttttc cctgatgttt
    29221 tcctcaatta agtattttat cctctggcct acaaggctgg aggatttttg ttattatatt
    29281 atttttttgc gtcttcaaac ttattacaaa aacataatta aaaacaaatt gacattttta
    29341 aattggcaat tacaaaatac agccttgcag ttatttcgat acctttagca aaggaaaagt
    29401 tgattcagat ataatttagc atttcaatca tctgacatca ttataaaaaa tgagattcac
    29461 ataaagataa aattattgtt ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat
    29521 tcggtgccaa gacaggactt gatacacaga atctcaactt ttatgctctc agactatatc
    29581 accacatgtt ttacaggctc agaaaatgaa cgatagatta tgctttgaag tgcacgataa
    29641 caagggttat ttcgtatttc cggatacctg gtttggccca ttgctggggg agtttgaaga
    29701 agtgcttgat gcatacgacg ctgatgaaat cagcgagacg agttacatca acaaactccg
    29761 acgccttgct caacgggagc cggactttat tgatatacac gctcatctgg catatgcatt
    29821 tctggaacag aatgccccgc gtaaggcact gaacgccgct cttaaaggac tggcggcagg
    29881 taatcgtatc atcccggaaa gtttctgtgg tgaaataatc tggatgcatc ccgaaaaccg
    29941 gccttatctg cgcgcgcttt atgcagcaat tctcgcaaat gtgcacttac agcgccatca
    30001 ggatgcggtt atgctcactg ataaaatact ggcatacaat cctgaagata atcagggggc
    30061 tcgctggttg ttgggttcag agctgttacg caccggcgat catgagcgag ccttcagtgt
    30121 actgaaagag catgctgatg aattttcacc ttactggtat gaactcggtc tcctgcattt
    30181 tctgaacggg gaacatgtta aggccgccac agcttttcgc catggctttg ccacaaacac
    30241 ttacatcgca gaaatgctgt gcggaaatct gcatccattt ccacttgctg tgtggcatga
    30301 cttctccggc tctctggata cggcagaaga ttactatgca acatactcac cattatgggg
    30361 acaatatccg gaagcattgc tgtttgtaaa ctggctctat aaccattcct ccgtgctgca
    30421 tgaacgatca gaaatcatta aatgcgccga aatgctgatt caggaagatg atttcgaaat
    30481 atgcgaaagt attttaagac agcaggagca cctgtggaaa agaattgaca agacactttc
    30541 tgaggaaatt gtacagaaat gcagaaatat gaacggtgaa tatatctggc cctggatatt
    30601 gcccttttcc gcagcaggga tgaaacattc aagaatccag tatcagtaga tattgcatta
    30661 gtgtaacttt aaaacaagta ataatttatt atccaataat aacactgtgt tccttcccca
    30721 ggtgattacc ctggggttat tttctgaaag tttcctttgc aaaaggatag cgttgtaaat
    30781 tactttaacg aattactcca tgaactcatt aaagttcttt cgtaatactt tatcgtcaaa
    30841 cacgttatag ctggcatcaa aaaccacaga tttaccctgc cagcgggtaa tcccctcctc
    30901 tggcgttttc ggttgaactg gtatccccgt ccaacacagg cagccattat aaacagtaaa
    30961 aaattgtatt cgctgttatg aaatcaatag tcctgacgta ctgttgaatg tgcttgtgtt
    31021 ctgaacctcg tacaaccagt acatttgata tcgaattata aacaaaacat tgcatccaac
    31081 ttttttaata tggacaatag ttgtccatac ggtggtttta tattcgataa atcaatggat
    31141 ttcattgagt cacaggactg gtaatggatg aaaatgcttt aaggtttgcc tcatactggc
    31201 gcaactcgct tgcagatgct gagtcaggaa agggctgttt tgaacggaaa gacgcccaaa
    31261 atttcactca ctggcatggg atagcggcgg gacgtcttga cgaagcgatt gtcaataaat
    31321 tttttaaggg agaaaaagac gatgtcgaaa ctgtcaatgt catcttacgc ccaaaagttt
    31381 atttccggtt actgcagcat ggtaaggacc gttccgcagg cacgcctgat attgttacac
    31441 cgatagtgac gccagccttg ctaagccgtg agggtttttt atatccgacg ccagcgacct
    31501 ccattcccag agacctgctt gaacctttgc caaaaggagc attttcgatt ggtgagattg
    31561 ggcagtatga caaatacaag acgacccata ctgatcctac ccacgtaatg tggacacggc
    31621 cctaagcgag attatggttt tcaaattgtt ccgggctgag accgccacag gcactgtgac
    31681 gacgccagcg attgtaatca cactcgatat aattaaacac cgttgcccgc attatttccc
    31741 ggctgctaaa gcgttccccg tggatacatt ccaccttcag cgaatgaaag aagctttcca
    31801 cacaggcatt gtcataacag tagcctttcg cactcatact gccacgcagg ttgtgtcgct
    31861 tcagcattgc ctgataatcc cctgaacagt attgaccacc acggtccgta tgaacaatga
    31921 cgctttccgg gcgtcttctc cgccacaacg ccatttgcag tgcatcacag gccagttgtg
    31981 ctgtcattcg cggtgacatc gaccagccaa tgacggcgcg tgaccacagg tcgatgacta
    32041 ctgcgagata caaccagccc tcatcggtac gcaagtacgt gatgtcaccc gcccacttct
    32101 ggtttcgggc cactggcgct gaagtcctgc tccagcagat tctccaatac gggcaggcca
    32161 tgtgcacggt agctgaccgg gctgaacttc cggctggctt tcgcccgcag cccctgacga
    32221 cgcaggctgg cggcaatggt tttaatattg aactccggca gttcgtcagc aaggcgggga
    32281 gcaccgtatc gctgctttgc ctcaaagaat gccttatgga cagcggcatc gcaggtgagc
    32341 cgaaactgtt ggcgcaggct catctggtga cgacgcctga gccagacata ccagccgctg
    32401 cgggcaaccc gaagtacacg acacatcgct ttgatgctga actctgcccg atgattttcg
    32461 ataaagacat acttcatttc aggcgcttcg cgaagtatgt cgcggccttt tggaggatgg
    32521 acagttcctc agcctgctcc gccagttgtc gtttaaggcg gacattttca gcggccagtt
    32581 cgctttcgcg ctctgacgaa ctcatttgtt gctgctgttt actgcgccag gcataaagct
    32641 gagattcata caggctgagt tcacgggctg cggcggccac accgatgcgt tcagcgagtt
    32701 tcagggcttc gttacgaaat tcaggcgtat gttgtttacg gggcttcttg ctgattgata
    32761 ctggttttgt catgagtcac ctctggttga gagtttactc acttagtcct gtgtccacta
    32821 ttggtgggta agatcaccct ccattaaaac taaaacgggc gctttctgat atatcatctg
    32881 taagcgcagt aataccatta gtagcacctc tcggcggcgc attgattatt gaatattata
    32941 actggaatgt tatatttctt gttatgatgc tgttcagtat tataataatg ttattatcac
    33001 caaagataac tcatgcaaat gagaaagtaa tatgtcaaaa cagtgcatcc tttgtgacca
    33061 aagagtttat ctgcggcact atacttgtct cattaatact cagttctcta ttttgctaca
    33121 tatcaatatc accatcaatt ttcatgcata cttatggcct gagtactttt agttactcaa
    33181 ttattttctc cttctcagtt ttatttttca taatcggaaa tcaattatca aagattgaaa
    33241 aattatccaa cctatggttt ttattaccca a
//
LOCUS       DADLTF010000028        33128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.028, whole genome shotgun sequence.
ACCESSION   DADLTF010000028 DADLTF010000000
VERSION     DADLTF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.028"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..223
                     /locus_tag="JN749_001271"
     CDS             <1..223
                     /locus_tag="JN749_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7367743.1"
                     /translation="PQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTVL
                     DGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            397..1166
                     /locus_tag="JN749_001272"
                     /pseudo
     CDS             397..1166
                     /locus_tag="JN749_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1199..2719)
                     /locus_tag="JN749_001273"
     CDS             complement(1199..2719)
                     /locus_tag="JN749_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7367744.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNERAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            2810..3529
                     /gene="tonB"
                     /locus_tag="JN749_001274"
     CDS             2810..3529
                     /gene="tonB"
                     /locus_tag="JN749_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HAY7367745.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(3569..3967)
                     /gene="yciA"
                     /locus_tag="JN749_001275"
     CDS             complement(3569..3967)
                     /gene="yciA"
                     /locus_tag="JN749_001275"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HAY7367746.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4072..4611)
                     /locus_tag="JN749_001276"
     CDS             complement(4072..4611)
                     /locus_tag="JN749_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HAY7367747.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(4641..5384)
                     /locus_tag="JN749_001277"
     CDS             complement(4641..5384)
                     /locus_tag="JN749_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HAY7367748.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(5412..5501)
                     /locus_tag="JN749_001278"
                     /pseudo
     CDS             complement(5412..5501)
                     /locus_tag="JN749_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            5741..6379
                     /gene="ompW"
                     /locus_tag="JN749_001279"
     CDS             5741..6379
                     /gene="ompW"
                     /locus_tag="JN749_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HAY7367749.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(6439..6945)
                     /locus_tag="JN749_001280"
     CDS             complement(6439..6945)
                     /locus_tag="JN749_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7367750.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDCVEAKK"
     gene            complement(6991..7491)
                     /locus_tag="JN749_001281"
     CDS             complement(6991..7491)
                     /locus_tag="JN749_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HAY7367751.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(7577..7756)
                     /locus_tag="JN749_001282"
     CDS             complement(7577..7756)
                     /locus_tag="JN749_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HAY7367752.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(8137..8943)
                     /gene="trpA"
                     /locus_tag="JN749_001283"
     CDS             complement(8137..8943)
                     /gene="trpA"
                     /locus_tag="JN749_001283"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HAY7367753.1"
                     /translation="MERYESLFAQLKERKEGAFIPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(8943..10136)
                     /gene="trpB"
                     /locus_tag="JN749_001284"
     CDS             complement(8943..10136)
                     /gene="trpB"
                     /locus_tag="JN749_001284"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HAY7367754.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(10148..11506)
                     /gene="trpCF"
                     /locus_tag="JN749_001285"
     CDS             complement(10148..11506)
                     /gene="trpCF"
                     /locus_tag="JN749_001285"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HAY7367755.1"
                     /translation="MQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLLI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(11510..13105)
                     /gene="trpD"
                     /locus_tag="JN749_001286"
     CDS             complement(11510..13105)
                     /gene="trpD"
                     /locus_tag="JN749_001286"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HAY7367756.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(13105..14667)
                     /gene="trpE"
                     /locus_tag="JN749_001287"
     CDS             complement(13105..14667)
                     /gene="trpE"
                     /locus_tag="JN749_001287"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HAY7367757.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDNALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            14940..15821
                     /gene="yciV"
                     /locus_tag="JN749_001288"
     CDS             14940..15821
                     /gene="yciV"
                     /locus_tag="JN749_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HAY7367758.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            15818..16438
                     /locus_tag="JN749_001289"
     CDS             15818..16438
                     /locus_tag="JN749_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAY7367759.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            16539..17411
                     /gene="rluB"
                     /locus_tag="JN749_001290"
     CDS             16539..17411
                     /gene="rluB"
                     /locus_tag="JN749_001290"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HAY7367760.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(17451..18041)
                     /gene="cobO"
                     /locus_tag="JN749_001291"
     CDS             complement(17451..18041)
                     /gene="cobO"
                     /locus_tag="JN749_001291"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HAY7367761.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(18038..18796)
                     /locus_tag="JN749_001292"
     CDS             complement(18038..18796)
                     /locus_tag="JN749_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HAY7367762.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            19016..20065
                     /gene="sohB"
                     /locus_tag="JN749_001293"
     CDS             19016..20065
                     /gene="sohB"
                     /locus_tag="JN749_001293"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HAY7367763.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKETKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(20101..20352)
                     /locus_tag="JN749_001294"
     CDS             complement(20101..20352)
                     /locus_tag="JN749_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HAY7367764.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            20732..23329
                     /gene="topA"
                     /locus_tag="JN749_001295"
     CDS             20732..23329
                     /gene="topA"
                     /locus_tag="JN749_001295"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAY7367765.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPSKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            23539..24513
                     /gene="cysB"
                     /locus_tag="JN749_001296"
     CDS             23539..24513
                     /gene="cysB"
                     /locus_tag="JN749_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HAY7367766.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            24808..24972
                     /locus_tag="JN749_001297"
     CDS             24808..24972
                     /locus_tag="JN749_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HAY7367767.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLTVFLGSALFWVV
                     VALLIWKVWG"
     gene            24975..25142
                     /locus_tag="JN749_001298"
     CDS             24975..25142
                     /locus_tag="JN749_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367768.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            25256..25351
                     /locus_tag="JN749_001299"
     CDS             25256..25351
                     /locus_tag="JN749_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367769.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            25515..28190
                     /gene="acnA"
                     /locus_tag="JN749_001300"
     CDS             25515..28190
                     /gene="acnA"
                     /locus_tag="JN749_001300"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HAY7367770.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(28254..28844)
                     /gene="ribA"
                     /locus_tag="JN749_001301"
     CDS             complement(28254..28844)
                     /gene="ribA"
                     /locus_tag="JN749_001301"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HAY7367771.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYADISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            29014..29778
                     /gene="pgpB"
                     /locus_tag="JN749_001302"
     CDS             29014..29778
                     /gene="pgpB"
                     /locus_tag="JN749_001302"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HAY7367772.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWTLVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29927..30235
                     /gene="lapA"
                     /locus_tag="JN749_001303"
     CDS             29927..30235
                     /gene="lapA"
                     /locus_tag="JN749_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HAY7367773.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            30242..31411
                     /gene="lapB"
                     /locus_tag="JN749_001304"
     CDS             30242..31411
                     /gene="lapB"
                     /locus_tag="JN749_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HAY7367774.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            31604..32341
                     /gene="pyrF"
                     /locus_tag="JN749_001305"
     CDS             31604..32341
                     /gene="pyrF"
                     /locus_tag="JN749_001305"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAY7367775.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNAHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            32341..32667
                     /gene="yciH"
                     /locus_tag="JN749_001306"
     CDS             32341..32667
                     /gene="yciH"
                     /locus_tag="JN749_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HAY7367776.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(32793..33011)
                     /gene="osmB"
                     /locus_tag="JN749_001307"
     CDS             complement(32793..33011)
                     /gene="osmB"
                     /locus_tag="JN749_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HAY7367777.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         8399 a         8005 c         8421 g         8303 t
ORIGIN      
        1 tccacagttt tcacctgaag cattaaataa tctgaaaggt cgagtggaaa cattcacaca
       61 agatttaaag gttatcaaac cgtcagtaaa tattcagcct cagacaatca atatcacaac
      121 tcagacggta cttgatggaa aggttattga tgaaagaacc acatctcata ttaatcgtat
      181 gcaggaagat accttgatta gctcagctta cccagaggaa taaacaatgt tagaactact
      241 aatatttatt gccacacatg gcaatctatt aatctttatc tagtatgacg attcaactat
      301 gaatagtcga atgctacgtg atttcttctg agcagatacc aacatcaaaa attagatttc
      361 acgtagctca cgcttaacag aataggaata acaacaatgt ttgacacaaa gcaaattaga
      421 tactcaggta aagatggtat ctattttcac cttagagata atgtagaatc ttttcttgcc
      481 ttaacagcta ctgaacagat ggactatgac tcaccgatga tggtgacaac caggctatgc
      541 aatcggggca aactatcact gataatgttc agagagcacc taagaccatc tctattagcg
      601 gtgttgtagt ggttgattcg acgggttcag ttctattaac cagacagggg caacttgtag
      661 agacctttat ttctacggtt gaacaatggc gagaccagaa gcaaatcatc actattgttt
      721 gtaaagatgg aatatctatc actgatagta tcattactca gtttaaagcg agtaaagagc
      781 ataccattgc taatggtctt cgtattcaga tgaccttcca aggtgtagaa tttcgttcaa
      841 tcgtagggca aactgatatt tcagcagcta ctggcaagac cgctaccacg aatgatggtg
      901 gtgctaccag taaaaagaac actggcaaca ctacaacgag tcttggaagc cctatgctga
      961 cctgtaaaga gctttttagc tattcggcaa gtgagttatc agatgaagcg ctaaaagccc
     1021 gtgtaacgtg ctctaagagc gttagcgtta aaaatggtga gagcaccttc acagcctcag
     1081 ctaatgagcg agcttcaaaa gtgcttaagt cgggtaatgc tttacggaag tactctgtga
     1141 atccgaataa gaagggcact tattgagaca taaaaagggg cttcatagcc cctgaaaatt
     1201 acagttgata agcatcaata tccagtagct ctttatcagc aatttcaaag attggtgacc
     1261 catcatttag cggtagcggg tctgtaggta tggtgatttc agtgtcgttc tgtagcgtta
     1321 tagttactga acccttaccc aatgtaagcc cttttagatg tttggacaaa attaattgac
     1381 actcgacacg acccgcttta gttgtcaggt caatatcttt aagctggggt agttccttat
     1441 cagtgagtga agccttacgc ttcatagtat caatcgtggt ctgtagttct gtgcgcttct
     1501 ggttaagttc cttaagctga atttttagtt catcaatatc atccatctca agaagtaggg
     1561 cggtagcttt cttgattctg gctgtgacaa tttcaacctc atgctgtaac gctgcaatct
     1621 tcctctctga ttctccatca ttccccacgt caatcatccc cagaaaatta tcaatcatcc
     1681 atcggtcaaa cggtttacgc ttcaacggtg gaacatcaca gcgaccgtct aagtggttat
     1741 tgcacctgta gaccccagca tacttattct ttgttccggt aggatggaca ctagccccac
     1801 actgacacct aaacagacct ttgaagatgt taatcgttag tgggttgtca cttgcaggtg
     1861 tacggccttt acgattctta tcaagaatct cttgagcttt attgaacgtt gcagcatcta
     1921 tagcggcttc gtagtagttc ggtatagcgg ggcggtcatt gcgctgtgat gctggtagtg
     1981 tacctatcgt tgtctctgac cttaagatag tacgcacaga atgaacgcta aatccattag
     2041 catctttacg tcgtgagaat gaactaatgt tattagcctg taggtgacgt gcaatgttat
     2101 gcagactttc accatctagc agcagcttga aaatcaagcg tacagtttca gcacgttcat
     2161 tgagtttaaa agtctcaggg tcaatccatc cgggaacatt ccttgttaaa cgttgttcac
     2221 cagcaagggc aagctttgca cgttgttccc atttctgact aacacggaaa gacttctgtt
     2281 tactctcctg atgcgctatg tagcttttaa cccgctgaat gattgagatt cctaaatcat
     2341 cggatagagc atcccttgtg tagattagcc catcctgtag agtgtgaatc tcgataccat
     2401 gagcaataat gcgattcatc aaggcttctg tttcaagatg ttcaaggcgg cctatgcggt
     2461 cgatagcttc gactagtaaa attgtccctt ctggaaactc ccctgattca atgcgggaga
     2521 gcatatcccc aaattgccct gataagtgtt tacctttgta accggataga ccttcatcat
     2581 tgaaagatgc cactacctga gcatcgctat tcttccttaa ccactgtttg aatagctcat
     2641 tttgtcgtct ggttgaatca gcaccttcag caccttgtgt ggctgatgag aatctcaagt
     2701 atgttattac ggttctcacg gtgataaact cccatgcaat tgattatcat ttaaagatga
     2761 acttataaac gttatggcta atgctacagt gccgaatagc tacaatacaa tgccccttga
     2821 tttacctcgc cgcttcccct ggccgacgtt actttcggtc tgcattcatg gtgctgttgt
     2881 ggcgggtctg ctctatacct cggtacatca ggttattgaa ctacctgcgc ctgcgcaacc
     2941 aatttctgtc acgatggttg cgcctgctga tctcgaaccg ccacaagccg ttcagccgcc
     3001 accggagccg gtggtagagc cagaaccgga acctgagccg atccccgaac cgccaaaaga
     3061 agcaccggtg gtcattgaaa agccgaagcc gaaacctaag ccaaaaccga agccggtgaa
     3121 aaaggtacag gagcagccaa aacgcgatgt caaacccgta gagtcgcgtc cggcatcacc
     3181 gtttgaaaat acggcacccg cgcgccccac atcaagtacg gcaacggctg caaccagcaa
     3241 gccggttacc agtgtggctt caggaccacg cgcattaagc cgtaatcagc cgcagtatcc
     3301 ggcacgagca caggcattgc gcattgaagg gcaggttaaa gttaaatttg acgtcacgcc
     3361 ggatggtcgc gtggataacg tacaaatcct ctcagccaag cctgcgaaca tgtttgagcg
     3421 tgaggtgaaa aatgcgatgc gcagatggcg ttatgagccg ggtaagccag gcagtgggat
     3481 tgtggtgaat atcctgttta aaattaacgg caccaccgaa attcagtaag cagaaagtca
     3541 aaagcctccg accggaggct tttgactatt actcaacagg taaggcgcga ggttttcctt
     3601 caggatcaac cgcgacatac ttaaataatg cttctgtcgc tttatagcgt tgcccgattg
     3661 gttcagacgc tacttttttc acccacactt caatattaat gctgaccgat gtcgtccctt
     3721 tctggacaca gcgtgcatag cagcacacca catcgccgac cgcaaccggc cgtaagaaag
     3781 tcattccttc aacccgcaca gtcactacgc gaccgtgggc aatttctttt gccagaatag
     3841 cgccgccaat atccatttgt gacattaacc aaccaccaaa gatgtcacca ttggcattgg
     3901 tatcggcggg catggctaaa gtacgtaaaa caagatcgcc ctgagggacg ttatgtgttg
     3961 tagacatggt aaaaccgact taaaaggaaa atcacaggcg cgatgctact atgattttcc
     4021 tgaggagaac agaggagata cagcgccagc cccgaaggac tggcagtctg gttaggattt
     4081 atcttcctgc ggcatgtggc ggtagatata gataccgctt aacaatgtga agattaaggt
     4141 aagggcggtc aggccaaaga ctttaaagtt gacccaaata ttttgcggca gccagaatgc
     4201 gatgtagatg tttgccagac cgcaaaggat aaagaaaaca gcccaggcca gatttagctt
     4261 cgaccatacc ggttgcggca gcgtgagttc tttacccagc atccgctgaa ttagcggctt
     4321 tttcatcacc cattggctga ctaacagggc taccgcaaac agagcataaa tgactgtaac
     4381 cttccattta ataaactcat cattgtggaa gaacagcgtc aagccaccga agacgaccac
     4441 cagaacaaaa gtgatcaggg ccatcttctc aaccttacga aagcgaaccc agctatagat
     4501 aagcacaatc gccgtggcga cgatcagcgc tgcagtagcc gcatagatgt cataaatctt
     4561 gtaaaacgcg aaaaagacaa ccagcggtaa aaaatcaaga aactgcttca ttttacgatt
     4621 ccgtaatcaa atgcttaagg ttattggcga atcaacatat acaggcggaa cagatagatg
     4681 agcaatatgg cagaaatcag gttgctcaag gtgttcgcca gtacggcacc aatttccggg
     4741 gttaatgcgg caaaagaaga ggcaaaaagc agcaacagtg tttttgccag caaccagctc
     4801 agtactgcgg gtgccaccag acgcatattc gcccaagtca gccgcatact gctacgcatc
     4861 gaggcaaaaa tgcccatttt gtcctgtacc agcatcaccg gtgccagggc cagtaaaatg
     4921 gccatgataa ttcccggaac gaccaccagc atgatgccaa tctgtactaa aagggtagtc
     4981 agaaaaatca gaataaataa cttgggcaat atcggcgcac tggcaccaat agctcgcagc
     5041 gcacagactc tctgacccgc agacaccagc tggataatta atattacgcc tccggcgaga
     5101 atggcgttac cgattaattc tgaaaaagtg gacgccgctg aagcctgcag caaaatttgt
     5161 tgctgttccg gtgacatatt ctgaaccagg tcgaacaacc cactactgcc gctaacgggc
     5221 acgccgtcat tgagctgcgc aagctgtgca tcactgggtg agaaaacatg ccctaacacc
     5281 actgtgataa acgcacatag caacgatacc aacagaatgg tcataaattg attacggaag
     5341 aaatttccgg tgtcacggta tacggactgc gccgtgatag acatgcactc tccttgagtt
     5401 ttatgcaggt gttaattagc gggcaattgt accctggtta agcctcgggt ggcagcatca
     5461 ggtcttgtat ggaaaagcat atctttgtaa agcggaggta attgctggcc ttcaggcgtg
     5521 ggcaaaccgc ggcaagtggc gatcgagtta atatttgcgt agagaaaata tttaaaaatt
     5581 gatttaaatc acattaacca ggattctcaa tgcaacttct aaattaatcc agatcaataa
     5641 atggtgaatt atcatatgta atgtgatcta tgtaggatca tttgttactc caatgtaggt
     5701 atattcgtca cgtttttata accataacga cggagcggat atgaaaaagt taacagtggc
     5761 ggctttggca gtaacaactc ttctttctgg cagtgccttt gcgcatgaag caggcgaatt
     5821 ttttatgcgt gcaggttctg caaccgtacg tccaacagaa ggtgctggta gtacgttagg
     5881 aagtctgggt ggattcagcg tgaccaataa cactcaactg ggcttgacgt ttacttatat
     5941 ggcgaccgac aacattggtg tggaattact ggcagcgacg ccgttccgcc ataaaatcgg
     6001 cacccgggcg accggcgata ttgcaaccgt tcaccatctg ccgccaacac tgatggcgca
     6061 gtggtatttt ggtgatgcca gcagcaaatt ccgtccttac gttggggccg gtattaacta
     6121 caccaccttc tttgataatg gatttaacga tcatggcaaa gaggcggggc tttccgatct
     6181 cagtctgaaa gattcctggg gagctgccgg gcaggtgggg gttgattatc tgattaaccg
     6241 tgactggttg gttaacatgt cagtgtggta catggatatc gataccaccg ccaattataa
     6301 gctaggcggt gcacagcaac acgatagcgt acgcctcgat ccgtgggtgt ttatgttctc
     6361 agcaggatat cgtttttaat tccgcacaaa aacgaccccg taatatacgg ggtcaataag
     6421 gacatggtat aaagggtatt atttcttcgc ttctacgcaa tcagtttcag aacgaattaa
     6481 aaatttctca gttgtttgcg gaatatgctg aatcagccag tcggccattt gcttttcctc
     6541 atttaaaatc gcttcgatga tcggaattga gactgtatca ccggcatttt ttgctgctgc
     6601 taatagtgag gtgtaacagg cgatttcaaa ttgctcgaag acatatccgc taatagagcc
     6661 tttaactatt tcatcagaag ggaatatacc accgattgac tgcccaagcg cagccatttt
     6721 actcatggaa tctttaatga ctgaacgtga aatgtcatta cgatcaagaa tagtttccag
     6781 ttgaacaatc tggtttttgg tttcactaag atgttgttca atacgagcgc gtagttcagg
     6841 ataattatct atacggctgg ccatggattc aagcatagat tcggcttgct tttccattgc
     6901 gtgggcgtca cgtaaccagt catgataatg ttcaatacga ttcatgaaaa tgtcctcttt
     6961 ataaaatgac taaaagttga aattcatatt tcaggcttta ttttcggctt tcttatttac
     7021 gttattaagg gccagatcag tcagtttgat gtcggtggcc ttttcttctt ccagggtttc
     7081 tttcagaagc ttcgctgctt tacggtaacc taattgttca gccagcgtcg ctaatgtccc
     7141 gtaactggca atctcataat gctcgacttt ctgtgctgcg gcaatcagtg cggcatcacg
     7201 cacttcgttt ttctcggtac tttcgatgac ctcattagct tcttcaataa gaccttccat
     7261 tgccacacat ttcatgcgct taattttcag atttgattct gattccacaa cttgatcaat
     7321 tcgttcaatc tgtccatgag tttcctcgag gtgcgcatga aaagcctgac ttaatttttc
     7381 atttgatgtt gctcttgcga gttttgccag tgcccggggt aattgttttt ctgcgctgta
     7441 ggtatctgaa agcagttgaa taaatacatc ttcaatggtc ttcatattca tatttttctc
     7501 cagtgaaatc actgcgagcc atacggcccg cagatgcaat tatctttatc agctgatatt
     7561 aataattaac ggtgaatcag gatttgtctg atttattacc accgctttgt tgaccgcctt
     7621 ttttacccgc ttcagatgcg cgttgcggat catttttaaa attaccgccg ctatgctgac
     7681 cgcctttacg gcctgcgtcg gatgccttct cacggtcttc ggcgaaattt cctgaaccac
     7741 cacgatgttc ggccatgtta tttctcccgt tgcgttgcat tgtttcatta atatgagtgt
     7801 tgtgtgtcga cactcattaa aattagtcgc taatgagaat tagtcaaatt aagcgcaacg
     7861 agaagataga gggaaaatat attttgagga acattctgga tatattaaca attacctgag
     7921 gaataaatga cttagagaaa attattgatt ttactggtgt tatgttgcgg gattaatttg
     7981 tttgattata ttactgttgg gcggaaaaat gacgtaagtt gacgttcgac cggggtaagc
     8041 gaaacggtaa aaagataaat attaaatgaa tttaggattt ttccggcttc attaaagaaa
     8101 gttaaaatgc cgccagcgga actggcggct gtgggattaa ctgcgcgtcg ccgctttcat
     8161 cggttgtaca aaaactttca gtgccgccag cattttctct ggctcattaa tatgttgctc
     8221 gatgatttta acaatggccg aaccagaaat cgcgcccgca gctcctgcat caatcgctgc
     8281 ttttacctga tccggggcgg aaataccaaa cccctgcaat ggaggtgcag cgttgtactc
     8341 tttcagcttc gcaaccagat gattgagggg taacgcggcg cggttttctg cgccggtcac
     8401 gcccgctcgt gacagcaaat atgtgtaacc acgaccgtaa gaggctatct ggcgcagcag
     8461 gtcgtcatcg gcatttggcg ggcagatgaa gataggtgcg acattatggc gcaacgcggc
     8521 ctggcggaag ggcgcggact cttcaactgg cacatcggca accagcaccg aatcgacgcc
     8581 gactttctcg cactgggcat aaaactcatc aatgcctttg ttaaacacca gattggcata
     8641 catcaacagg ccaatgggaa tggtcgggtg tttctggcga atcagtgcca gcatttcaaa
     8701 acattgtgcc ggagtcacac ctgccgcaaa ggcgcgcaga gtggcgtttt gaatggtcgg
     8761 gccatccgcc agtgggtcgg agaaggggat acctaactcc agcgcgtcag caccggcttc
     8821 aattagcgta tcgataattt tcaatgactg ctcaatgccc ggatcaccga gcgtgacgaa
     8881 aggaatgaat gcgccttctt tgcgctcctt caactgggca aacagagatt cgtagcgttc
     8941 catcagattt cccctcgtgc tttcaaaata tcgtgaacgg tgaagatgtc tttatcgccg
     9001 cgaccggaaa ggttaaccac cagtagctgc tctttatccg ggttttcgcg catcattttc
     9061 agcgcatggg ccagggcgtg ggaggattcc agcgccggga tgatcccttc gtgcaggcac
     9121 agcgttttga aggcttcaag ggcttcatca tcggtaatag acacgtaatc agcgcgtcca
     9181 gtgctgttaa gatacgcgtg ttgtgggccg acagacggga aatccagtcc ggcggagatg
     9241 gagtaagatt cttcaatctg cccgtcttcg gtttgcatca tcggcgcttt cataccgaaa
     9301 taaatgccca cgcgaccatg ttttagcggt gcgccgtgct cgccagtttc gataccgtga
     9361 ccaccaggct ccacaccaat caggccgacg ttggtttcat tgatgaaatc agcaaacatg
     9421 ccgatggcat tcgaaccgcc gccaacacag gcgataacgg catccggcag gcgaccttct
     9481 ctttccagaa tctgcgcttt ggtttcttcg ccaatcatcc gctgaaactc acggacaatg
     9541 gtcggataag gatgcgggcc agccgcggtg cccagcatat agtgcgcggt ttcataactg
     9601 ccggaccagt cgcgtagcgc ctcattacag gcatctttca gcgtggcgga accgctatgt
     9661 accgggatca cttccgcacc cattaagcgc atacgaaaaa cgttaggcga ctggcgttca
     9721 acgtctttgg cacccatata aatacggcat ttcaggccga gcagggcgct ggcaagggcc
     9781 gacgccacgc catgctgacc ggccccggtt tcggcgatga tttcggtttt acccatccgc
     9841 ttcgccagca acgcctgccc cagcacctgg ttggttttat gtgcgccgcc gtgcagcaaa
     9901 tcttcacgct tgagatacag cgtggtgttc gtcccggctg taatgttctg gcatttggtc
     9961 agtgcggttg gacgcccggc atagtttttc agcaggtcgt tgaactgggc ttgaaattca
    10021 ggatcttttt gcgcgctgac aaaagcttct tccagctggc gcagagcagg catcaggatt
    10081 tgtggcacgt acatgccgcc aaactcacca aaataggggt taagtaatgt tgtcattgtt
    10141 cctttcctta atatgcgcgc agcgtctgga aaaccgaggc caaaagacgt gcgtctttga
    10201 tgcccggttg cgactctaca gcagaattaa aatcaagtcc ggcgcagccg gtttgtgccg
    10261 cttccacgca gttatctgcg cctaagcccc ccgccagcag aacgttgcca agcgattgac
    10321 catttaatag tgaccagtca aaacgttgcc cgcttccacc ctggccgttg tctaaaacat
    10381 atttatcaac gtgctgaaac tcgcgggcgg gcagggtttc accgacgctt aatgctttcc
    10441 agatggcaac atgtgctggc agagcttcac gcagcgtatc gatatacagc tgatcttcat
    10501 taccatgcag ttgcactgcc gccagcgata acaccttagc tttgtccacc acatcggcaa
    10561 tatcgtgatt gcggaacacg ccaacatact gcaacggtgc cgcagccatc acttcctgcg
    10621 cctgttcaac gttgacgcaa cgcggtgatg tcgcaacaaa aatcaaccca ccgtaaatcg
    10681 cgcccgcgtc ataagctgct ttagcatctt gcccacgcgt caggccacat actttattct
    10741 cacccagcaa cacccggcgc acggcggcgt gcaaatcgtc atgggccatc aacgccgaac
    10801 caatcagaaa accgttagcg aagtggctta actcgcgcac ctgagcgtaa gtattgatgc
    10861 cggattcgct gattaccgtt acgttgtgcc ccagtttcgg cgcaagctcg cgggtacggt
    10921 tgagatcaat cgacaaatca cgcagatcgc ggttgttgat gccaacgacc tttgccccta
    10981 atgcaatggc gcgctccagt tcctcttcat tactgacttc ggtcagcacc cccatctcca
    11041 gactgtgagc gacggctgca agctggcgat attggtcgtc atccagtact gaaagcatta
    11101 ataagcaggc atcggcctgg taatagcgcg ccagatagat ctggtaaggg tcgataatga
    11161 agtctttaca taaaatcggc tgcggggcga tttggctgac gatgaggagg aaatcaaagc
    11221 tcccctgaaa atatttctca tcagtcagca ccgaaattgc cgaagcgtaa tgtttataaa
    11281 tggcggcaat gcgtgccgga tcgaaatcat cacggatcac gccttttgac ggcgacgctt
    11341 ttttacactc cagaataaac gccgtgcgtg caccttgtag cgcatcataa aaatgtcgtg
    11401 tgctcggctg aacctcattc tgaaaactgg ccagcggttg ctgctgtttg cgggtttcta
    11461 cccaaatcgc cttgtctgcg acgattttcg ctaaaacggt ttgcatcatt taccctcgtg
    11521 ccgccagcgc ggtaactctg tcgtaagcgg aaccactgcg cagtacctca agaacggttt
    11581 gcgcattggc ttgcagatct tcatggccat gcaggcgcat taacatggcg acgttcgcag
    11641 cgacggctgc ttcatgggcg gcgtcgcctt taccttgtaa caagcgtgtt aaaatgtcac
    11701 ggttttcttc aggtgttccg cctgccagtt gctcctggtg gtagggagtc aggccaaaat
    11761 cttcagcggt caattgatag ctcttaattt tgccgtcatg cagctcggca acgattgtcg
    11821 gcgcgtgtaa tgaaacttca tccatcccgc cgctgtgcac caccgccgcg cgttgatacc
    11881 ccagcacgcg caaggtttcg gcaatcggca gcaccagttc cggactataa acaccaatta
    11941 acgccagcgg cggatgcgcc gggttaatca atggccccag cacattgaac agggtgcggg
    12001 ttttcagttg ctggcgaacc ggcatcgcgt ggcggaatcc ggtgtgatat ttcggcgcaa
    12061 agaggaaaca tacgcctaac tcgtccagcg cctggcgcga tttattggcg ttcatatcaa
    12121 gattaatacc gaatgccgcc agcagatccg acgagccgga tttactggag acgctgcggt
    12181 taccgtgttt cgccactttc agcccacagg ccgcggcgac aaacgcactg gcggtagaaa
    12241 tattgatact gttgctgccg tcaccgccag taccgacgat atcagcaaac agataatccg
    12301 ggcgcgggaa cggcgctgcg ttttccagta gcgcggttgc tgccccggcg atctcgttcg
    12361 ggtgctcacc acgaattttc atgctcacca gcgccgccgc cagttgttcc ggcttcagct
    12421 cgccacgcac caccgctgaa aacagctggt ggctttcttg ttggctaagc gtctgcgcct
    12481 gatacagttt ttccagaatc ggttgcagcg tgttggctgg ctctagtttc tgctgcgccc
    12541 aggccagcgt ttgttccagc aggcgagcgc cctgggtggt gagaatggat tccggatgga
    12601 actggaatcc acaaacgcga tccgcatcgt gacgtactgc catcaccatg ccattaaaat
    12661 gggcgttgat ggttaaaccg gccggaatgt tactgccaac cagcgagtga taacgcgcca
    12721 ccggcagcgg gtttgttaat ccggcaaaca tcgcctgacc gtcatgttca atgctcgacg
    12781 ctttaccgtg aagaatttcg cccgcctgac cgacatagcc cccgtaagct tcgacaattg
    12841 cctgatgtcc gaggcaaatg ccaataatgg gcagcttgcc acgcaagcgg gtgaggagtt
    12901 ccggcataca accggcttcg ctcggcacac cggggccagg agaaagcatc agtaccggat
    12961 tgctcatcgt cgccaggcgt tcaattaagg tctgcgccgg aatatggttg cggtaaatca
    13021 ccacgttatg accattgctg cgcaactgat ctgccaggtt gtacgtaaaa gagtcgatat
    13081 tatcgagcag cagaatgtca gccatcagaa agtctcctgt gcatgatgcg cggtggcaat
    13141 agcgcgcagt acagcgcggg ctttgttacg ggtttcgtcg gcttccgact gcggaacaga
    13201 atcaaggact acaccagcac ccgcttgcac ggtggcgata ccgttttcca ccagcgccga
    13261 gcggatcaca atgcaggtgt cgagatcgcc atgcgcggtg aaataaccta ccgcgccgcc
    13321 gtagctgccg cggcgacgac cttccgcctc ggcaattaac tgcatagcgc gtactttcgg
    13381 cgcaccgctt aacgtcccca tattcataca ggcgcgataa gcgtgcaggg cgtcaagatc
    13441 gtgacgcagt tcgccgacta cgcgagagac gaggtgcatc acataggaat aacggtcaac
    13501 tttggtgaga tcggcgacgt agcggctgcc aggggtgcaa atgcgtgcca gatcattacg
    13561 ggcgagatca accagcatca gatgttcaga cagctcttta tggtcagtac gcatttccag
    13621 ttcaatacgg ctgtcgagat ctctgtccag tgaaccatcg gcgcgacgac cgcgtgggcg
    13681 tgttccggca atcgggtaga tctcaatctg gcggctggtg gcatcatact tgagcgagct
    13741 ttccggcgac gcgccaaata gggtgaaatc attatcctgc ataaaaaaca tgtacgggct
    13801 gggattactc tttttcagca cgtaataggc cgccagcggt gacgggcagg gcagagagaa
    13861 acggcgagat ggcaccacct ggaaaatttc tccagcgcga atcgcttttt gcaacaaacg
    13921 cactacgcca ccgaactctt catcgctctg attacattca caacgcatat gcggcacgga
    13981 aaccactggc agcggcggcg cggcttcggt cagttgctga cgtagttcgt tcaggcgagc
    14041 agtgagacgt tgtttttctt cttcattcgg agcaaacagg ctggcctgaa tacgggtgct
    14101 ttttttctga tggtcaatca ccatcagcgt ttcagcgaga taaaaacaga aatcagggca
    14161 gttattttcc gctgacagtt gcggtaaatc ttcaaatccc gccacaaggt cataagagaa
    14221 caggccgccg aagaacattg cttctcgttc ttccttcggt acattcaaca gattctgcaa
    14281 taaacggaaa gcgtcaaaaa ccgaaaggga gcataagcgg gcgtcttcat ccagcagtgg
    14341 actgacaggg gggaagcgca gcacacggca gtttggtgat tgttcacttt ccacacccgc
    14401 aggcagggcg ttatccagta gtgccagcag ggcttcgcca ttgccggaaa gtgcctgaat
    14461 tgtgacggtg tcacctaatg ctgtaatgtg cagcgcattg tctatcagca gcaggctttt
    14521 taaatcatct ttgctgtcga tatctgcgga ttccagcagc agcgttgccg gacgattccc
    14581 acacaactgg tgaaaaagcg cagtcgggtt gtcgcgataa gcgccttcgc aggttagcag
    14641 ttcgagagtc ggtttttgtg tttgcattgt tattctctaa ttttgttcaa aaaaaagccc
    14701 gctcattagg cgggctgggt atctgattgc tttacgcatg gtgaatacac tgcccgtttc
    14761 aggaagtggc caccaacctt tcagtacgaa aattgctttc attgtcgata ccatttttac
    14821 gtgaacttgc gtactagtca actggttcga tgattaattg tcaacagctc atttcagaat
    14881 atttgccaga cccgttatga tgtcggcgca aaaaacatta tccagaacgg gagtgcgcct
    14941 tgagcgacac gaattatgca gtgatttacg acctgcacag ccataccaca gcttccgatg
    15001 gctgcctgac gccagaagca ttggtgcacc gtgcagtcga gatgcgcgtc ggcaccctgg
    15061 cgatcaccga ccatgacacc acagctgcaa tcgcgccagc aagagaggaa atttcacgtt
    15121 cgggactggc gctgaatctt attcccggcg tggaaatttc cacggtctgg gaaaatcatg
    15181 aaattcatat tgtcgggctg aatattgata ttactcatcc gctaatgtgt gagttcctcg
    15241 cgcagcagac agaacggcgc aatcagcggg cgcagcaaat tgccgaaaga cttgaaaaag
    15301 cgcaaatacc tggggcgctg gaaggcgcac aacgactggc gcagggcggc gcagtgacgc
    15361 gcggtcattt tgcgcgtttc cttgtggagt gcggcaaagc cagttcaatg gcagatgtct
    15421 ttaaaaagta tctggcgcgc gggaaaaccg gatacgttcc gccacagtgg tgtacaatag
    15481 aacaagctat tgatgtcatt catcattctg gcggtaaggc ggtgctagct catcctgggc
    15541 ggtacaatct tagtgctaaa tggctgaaaa gattggttgc gcattttgcc gaacaccacg
    15601 gcgacgcgat ggaagtcgcg cagtgccagc aatcgcccaa tgaacgcacc cagctggcca
    15661 cacttgcgcg tcagcatcat ttatgggcat cacaaggatc tgattttcat cagccatgcc
    15721 cgtggatcga actgggtcgt aaactctggc tgcccgcagg cgttgaaggc gtctggcagc
    15781 tatgggaaca gccgcagaac accacagaga gggaattatg agccagtttt tttatattca
    15841 tcctgataac ccgcagcaac gtctgatcaa ccaggcggtg gagatcgtgc gtaaaggcgg
    15901 ggtgattgtc tatccaactg attccggcta tgcgctcggc tgtaaaattg aagataaaaa
    15961 cgccatggag cgtatttgtc gtattcgcca gctgccggac ggtcacaact ttaccctgat
    16021 gtgtcgcgat ctttctgaac tctcgaccta ttcattcgtg gataacgttg cgtttcgttt
    16081 gatgaaaaac aacacgccgg gcaactatac cttcatcctg aaggggacga aggaagtgcc
    16141 acgccgtctg ttgcaggaga aacgcaaaac catcggtatg cgtgtgcctt ctaacccgat
    16201 tgcgcaggcg ttgcttgaag cactgggcga accgatgctt tccacttcgc taatgctgcc
    16261 aggcagcgaa tttaccgaat ccgatcccga agaaattaaa gaccgtctgg aaaaacaggt
    16321 agatttgatt attcacggcg gctatctcgg gcagaaacca acaacggtta ttgatcttac
    16381 cgatgatacg ccggtcgtgg tgcgtgaagg cgtaggtgat gtgaagcctt tcttataagt
    16441 gcgtaactct gtatactacg cggccttaaa atggcgctat acggcgccat tgattctcct
    16501 gttcgacgcc tgtgaaggcg acaccaaaag gaagctctat gagcgaaaaa ttacagaaag
    16561 tgctggcccg cgctggccac ggctcccgcc gtgaaatcga atctattatt gaagccggtc
    16621 gtgtgagtgt tgatggcaaa attgctaaac tcggcgatcg cgttgaagtt acccctgggc
    16681 tgaaaattcg tatcgatggt catctgattt cggtacgtga gtccgctgaa caaatttgtc
    16741 gcgttctggc ctattacaaa ccggaaggtg agttgtgtac ccgtaacgat ccggaagggc
    16801 gtccgacggt gtttgaccgt ctgccaaagc tgcgtggcgc acgctggatt gccgtaggtc
    16861 gtctggacgt taacacctgt ggtctgctgc tgttcaccac cgatggtgaa ctggcaaacc
    16921 gtttaatgca tccaagccgt gaagttgaac gtgaatacgc cgtgcgtgta tttggtcagg
    16981 ttgacgacgc gaaactgcgt gatttgagtc gtggcgtgca actggaagat ggtccggcag
    17041 cttttaaaac catcaagttc agcggcggcg aagggatcaa ccagtggtac aacgtgactc
    17101 tgaccgaagg ccgtaaccgt gaagttcgtc gtctgtggga agcggttggt gtgcaggtga
    17161 gccgcctgat ccgtgttcgt tacggtgata tcccactgcc aaaaggtctg ccacgcggtg
    17221 gctggacgga gctggacctc gcccagacta actatctgcg cgaactggtg gagcttccgc
    17281 cagaaaccag ctctaaagtc gctgtagaaa aagaccgtcg tcgcatgaag gctaatcaga
    17341 ttcgtcgtgc ggtgaaacgt cacagtcagg tgagcggcgg tcgtcgttct ggcgggcgta
    17401 ataacggtta atcaaagtat gccggacgtc atatccggca tttttacaga ttaataatca
    17461 atccctattt gcgccttaac accggcatca aacgcatgtc tgatggggcg cagctcactt
    17521 accgtatccg ccagttcaag aatatcccga tgacagcctc gacccgtgat aatcaccgtc
    17581 tgttgatgtg gacgttcatt taacgcctgc accacttctt ccagtggcaa atagtcatac
    17641 gccaccatat acgtcagttc atcaagcaaa accatatcca gtgaggaatc agcaagcatc
    17701 cgctttgcat gttgccagac ttcgcggcag gcggcggtat cagactcgcg gttttgtgta
    17761 tcccaggtaa agcccgttgc catcacctga aactcaacgc catgtggctc cagcagattg
    17821 cgttcgccat taggccaggt gcctttaata aactgcacga cgcccacttt ttttccgtga
    17881 ccaactgcgc gtgttgccgt accaaatgcc gcggtggttt tgccttttcc attgccggta
    17941 aagacgatga taataccgcg ttcatcctgg gcctgggcca cacgagcatc tactttttct
    18001 ttcactcgct gctgacgctg ttggtagcgt tcatcactca ttgggaaatt cctggtttac
    18061 ggcccggttg ggcgtcaaag gtcatgccgg ttttacggcg gctgtcatcg cccatcagcc
    18121 agaggtagag cggcatgata tcagcgggtg ttttaagttt ctgtggatct tcggtcggga
    18181 aggcgcttgc gcgcattgcg gtgcgcgtac cgcctgggtt aatgcagttg acgcgcaggc
    18241 gctgctgata ttcatcggcc agtacctgca tcatcccttc ggtggcaaat ttcgacgctg
    18301 catatgcacc ccagttggct cgtccctgac gtccaacgct tgatgaagta aagaccagtg
    18361 aaccggcgtc cgatttgagt aataaaggaa gcagtgcctg ggtgagcata aaggtggcat
    18421 taacgttgac ctgcatgacg tcctgccaga cctgcggatt ttgttcgctc attgggcaaa
    18481 catcgccgag caatccggca ttatgcaaaa caccatccag acgcggataa ttaacgacaa
    18541 tgcgctgtgc cagttgttgg caattttcgg acgtgcaggt cagcaaatcg agaataaacc
    18601 actgtggctg acgcccagtt tcttcgttta tgtggctggc tacctgacgt aatttttctt
    18661 cattacggcc caacagaatc actgtcgcac catagcgtgc atacgtcatc gcggcttcac
    18721 gaccaatccc atcgctggct cccgtcacca ggataatgcg atcattgagt aaatcttgtt
    18781 ttggctggta atgcatggct actcctcaac gacgttgtct gtcgcgctgc cagtgtactt
    18841 tatgactttg gggctttatg cctgaaacag aagggcattt caatcagcct ggggcgataa
    18901 caccgcaaaa tggatacttt gtcatacttt cgctgcaata acatctctgc gagacggctt
    18961 aacatgcctg ttgtaaactg tgagccaaag cgttgtttaa ccaaggtggg gactcgtgga
    19021 attgttgtct gaatatggtt tgtttttggc taaaatcgtt accgttgtgc tagcgattgc
    19081 ggcgattgcc gccattattg tcaatgttgc tcaacgtaat aaacgccagc gtggcgagtt
    19141 acgggtcaac aatctcagcg aacagtataa ggagacgaaa gaagaactgg ccgcggcgct
    19201 gatggactca catcagcaaa aacagtggca caaagcgcag aagaaaaagc acaagcaaga
    19261 agcgaaagca gcaaaagcga aagccaaact gggcgaggtg gcaactgaca gtaaacctcg
    19321 cgtctgggtg ctggatttta aaggcagcat ggacgcccat gaagtgaact cgctacgtga
    19381 agagataacg gcggtactcg cagcattcaa accgcaggat caggttgtgc tccgtctgga
    19441 aagccctggt ggcatggtgc atggttacgg gttggcggct tcgcagctgc agcgtctgcg
    19501 tgataaaaac attcctttaa ctgttacggt agacaaagtc gctgccagcg gcggttacat
    19561 gatggcctgt gtggcggaca aaattgtttc cgcaccgttt gctattgtgg gttccattgg
    19621 ggtggtggcg caaatgccca actttaaccg cttcctgaaa agcaaagata ttgatatcga
    19681 actgcacacc gccgggcagt ataagcgcac gctgaccttg ctgggtgaaa ataccgaaga
    19741 agggcgggag aaattccgcg aagagctgaa cgaaacgcat cagttattta aagattttgt
    19801 gaagcgtatg cgtccgtctc tggatattga acaggtggca acgggtgaac actggtacgg
    19861 acaacaggcg gtagagaaag gcctggttga tgaaatcaac accagtgatg aagttattct
    19921 tagcctgatg gaaggccgtg aagtggttaa tgtacgctat atgcagcgta aacgactcat
    19981 tgaccgattc accggcagcg cggcagagag cgccgatcga ttgttgctac gctggtggca
    20041 gcggggtcaa aagccattga tgtaaaagac aaacgcgagg ctaagacctc gcgttttgct
    20101 ttaatcaacc agatgatatt tttctgaaag cacatgggcc aggtgtttga acatattaaa
    20161 caccgcggtg cttttggctg ttggcaatcc ttgttcatct aaaaagtagt cgccggtaaa
    20221 taccagcacg ccattacgct gcgtgacgcc ggtggcttca atccctgcga gcgtatcctc
    20281 atgctcacga atgattttgt tagcctcttt caacagcgtt tcgcggtcga tgggttgtgt
    20341 ctctttgttc attatttact ccttaaacaa ggacattagt ctacgccagg catggcttgc
    20401 agacaaatat accacgctgg tggcaagagc gccttactgg caactttgga ttttgcatgc
    20461 taataaagtt gcgtatcgga ttttatcagg tacagtgtga cgctttcgtc aatctggcaa
    20521 tagatttgct tgacattcga ccaaaattcc gtcgtgctat agcgcctgta ggccaagacc
    20581 tgttaactca gtcacctgaa ttttcgtgaa cagagtcacg acaagggggt gatatccgca
    20641 gagagcgagt ccatatcggt aactcgttgc cagtggaagg tttatcaacg tgcgacgcat
    20701 tcctggaaga atcaaattag gtaaggtgaa tatgggtaaa gctcttgtca tcgttgagtc
    20761 cccggcaaaa gccaaaacga tcaacaagta tctgggtagt gactacgtgg tgaaatccag
    20821 cgtcggtcac atccgcgatt tgccgaccag tggctcagct gccaaaaaga gtgccgactc
    20881 tacctccacc aagacggcta aaaagcctaa aaaggatgaa cgtggcgctc tcgtcaaccg
    20941 tatgggggtt gacccgtggc acaattggga ggcgcactat gaagtgttgc ctagtaaaga
    21001 gaaggtcgtc tctgaactga aacaactggc tgaaaaagcc gaccacatct atctcgcaac
    21061 cgaccttgac cgcgaagggg aagccattgc atggcacctg cgggaagtga ttggtggtga
    21121 tgatgcgcgc tatagccgag tggtgtttaa cgaaattact aaaaacgcga tccgccaggc
    21181 atttaacaaa ccgggtgagc tgaatattga tcgtgttaat gcccagcagg cgcgtcgctt
    21241 tatggaccgc gtggtggggt atatggtttc gccgctgctg tggaaaaaga tcgctcgtgg
    21301 tctgtctgcc ggtcgtgtgc agtcggtggc ggtccgcctg gtggtagagc gtgagcgtga
    21361 aattaaagcg ttcgtaccgg aagagttctg ggaagtcgat gccagcacga ctacgccatc
    21421 tggtgaagcg ttggcgttgc aggtgactca tcagaatgac aaaccgttcc gtccggtcaa
    21481 caaagaacaa actcaggctg cggtaagtct gctggaaaaa gcgcgctaca gcgtgctgga
    21541 acgtgaagac aaaccgacaa ccagtaaacc tggcgctcct tttattacct ctacgctgca
    21601 acaagctgcc agcacccgtc ttggttttgg cgtgaaaaaa accatgatga tggcgcagcg
    21661 tttgtatgaa gcaggctata tcacttacat gcgtaccgac tccactaacc tgagtcagga
    21721 cgcggtaaat atggttcgcg gttatatcag cgataatttt ggtaagaaat atctgccgga
    21781 aagtccgaat cagtacgcca gcaaagaaaa ctcacaggaa gcgcacgaag cgattcgtcc
    21841 ttctgacgtc aatgtgatgg cggaatcgct gaaggatatg gaagcagatg cgcagaaact
    21901 gtaccagcta atctggcgtc agttcgttgc ctgccagatg accccagcga aatatgactc
    21961 cacgacgctg accgttggtg cgggcgattt ccgcctgaaa gcacgcggtc gtattttgcg
    22021 ctttgatggc tggacaaaag tgatgcctgc actgcgtaaa ggcgatgaag atcgtatctt
    22081 accggcagtc gataaaggcg atgctctgac gctcgttgaa ctaacaccag cccagcactt
    22141 taccaagccg ccagcccgtt tcagtgaagc atcgctggtt aaagaactgg aaaaacgtgg
    22201 tatcggtcgt ccgtctacct atgcgtcgat catttcgacc attcaggatc gtggctatgt
    22261 gcgagtagaa aatcgtcgtt tctatgcgga aaaaatgggc gaaatcgtca ccgatcgcct
    22321 tgaagaaaat ttccgcgagt taatgaacta cgactttacc gcgcagatgg aaaacagcct
    22381 cgaccaggtg gcaaatcacg aagcagagtg gaaagctgta ctggatcact tcttctcgga
    22441 tttcacccag cagttagata aagctgaaaa agatccggaa gagggtggta tgcgcccgaa
    22501 ccagatggtt ctgaccagca ttgactgccc gacttgtggt cgcaaaatgg ggattcgcac
    22561 agcgagcacc ggggtattcc ttggctgttc tggctatgcg ctgccgccga aagagcgttg
    22621 caaaaccacc attaacctgg tgccggaaaa cgaagtgctg aacgtgctgg aaggcgaaga
    22681 tgctgaaacc aacgcgctgc gcgcaaaacg tcgttgcccg aaatgcggca cggcgatgga
    22741 cagctatctc attgatccga aacgtaagtt gcatgtctgt ggtaataacc caacctgcga
    22801 cggttacgag atcgaagagg gcgaattccg cattaaaggt tatgacggcc cgatcgttga
    22861 gtgtgaaaaa tgtggctctg aaatgcacct gaaaatgggg cgtttcggta aatacatggc
    22921 ctgcaccaac gaagagtgta aaaacacacg taagatttta cgtaacggcg aagtggcacc
    22981 accgaaagaa gatccggtgc cattacctga gctgccgtgc gaaaaatcag atgcttattt
    23041 cgtgctgcgt gacggtgctg ccggtgtgtt cctggctgcc aacactttcc cgaaatcgcg
    23101 tgaaacgcgt gcgccactgg tggaagagct ttatcgcttc cgcgaccgtc tgccggaaaa
    23161 actgcgttat ctggccgatg cgccacagca ggatccggaa ggtaataaga ccatggttcg
    23221 ctttagccgt aaaaccaaac agcaatatgt ctcttcggaa aaagacggaa aggcgactgg
    23281 ctggtcagca ttttatgttg atggcaaatg ggttgaagga aaaaaataac ctttaattct
    23341 atcaggtttt tataaacaaa gggtcgcgaa agcggccctt ttttattgca tattattttt
    23401 tcttcacacc tatacactaa ggctataaat gatatagtgg ttatagttag cacctttttt
    23461 attattaaat cgtattagtc acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt
    23521 ctaagtggat ggtttaacat gaaattacaa caacttcgct atattgttga ggtggtcaat
    23581 cataacctga atgtctcatc aacagcggaa ggactttaca catcacaacc cgggatcagt
    23641 aaacaagtca gaatgctgga agacgagcta ggcattcaaa ttttttcccg aagcggcaag
    23701 cacctgacgc aggtaacgcc agcaggacaa gaaataattc gtatcgctcg cgaagtcctg
    23761 tcgaaagtcg atgccataaa atcggttgcc ggtgagcaca cctggccgga taaaggctcg
    23821 ctgtatatcg ccaccacgca tacccaggca cgctacgcgt taccaaacgt tatcaaaggc
    23881 tttattgagc gttatccccg cgtttctttg catatgcacc agggcccgcc gacacaaatt
    23941 gctgatgccg tctctaaagg caatgccgat ttcgctatcg ccacagaagc gctgcatctg
    24001 tatgaagatt tagtgatgtt accgtgctac cactggaatc gggctattgt agtcactccg
    24061 gatcacccgc tggcaggcaa aaaagccatt accattgaag aactggcgca atatccgttg
    24121 gtgacatata ccttcggctt taccggacgt tcagaactgg atactgcctt taaccgcgca
    24181 gggttaacgc cgcgtatcgt tttcacggca acggatgctg acgtcattaa aacttacgtc
    24241 cggttagggc tgggggtagg ggtcattgcc agcatggcgg tggatccggt cgccgatccc
    24301 gaccttgtgc gcgttgatgc tcacgatatc ttcagccaca gtacaaccaa aattggtttt
    24361 cgccgtagta cttttttgcg cagttatatg tatgatttca ttcagcgttt tgcaccgcat
    24421 ttaacgcgtg atgtcgttga tgcggctgtc gcattgcgct ccaatgaaga aattgaggtc
    24481 atgtttaaag atataaaact gccggaaaaa taatttcttg cgttattttc ggcacctttt
    24541 atgtagcgaa ggtgccggaa tatattctct tttgttactt atttctatac cgtgcacaat
    24601 atttttatcc ctcatattta tagggtaaat tacctgtaaa gtagtgtcta attacccgcc
    24661 tcgcaaattc cagttttcaa atatctattt caggtcaaaa gattgaatta ttcactggag
    24721 acgattcgta aattcgctgg atttttgact aaagtttctt taggatttgt ctcatcgtat
    24781 gattaattac accaatcatt caacgtcttg ctgataagtg atggcgatta tatgaggtta
    24841 gcaatgcctt ctggaaatca ggaaccccgc cgtgatcctg aattaaaacg taaagcctgg
    24901 ctgacggttt ttcttggttc tgcacttttc tgggtggttg tcgcactgct gatttggaaa
    24961 gtgtggggat aactatggtc ggtcaggagc aactggagtc gtcaccatta tgccagcata
    25021 gtgacaatga accagaaacg aaacgggaat gttccgtcgt tattccagac gactggcaac
    25081 taacatcgca gcagcaagcc tttatagaac tgtttgctga agatgatcag ccgaaacaat
    25141 aattatcatc attcttatta cccattttta atgaattaaa gggcttttaa tacaccgcag
    25201 caataacagc ttgagttatc tcaacacaaa ataataaccg ttaagggtgt agcctatgat
    25261 caacacaaat atgaaatatt ggtcctggat gggcgcgttt tctctgtcga tgctcttctg
    25321 ggccgaactc ctctggatca ttactcactg atccttgacc ccgctgcggc ggggttgtca
    25381 tttgctttgc cacaaggttt ctcctctttt atcaatttgg gttgttatca aatcgttacg
    25441 cgatgtttgt gttatcttta atatttaccc tgaagagaat cagggcttcg caaccctgtc
    25501 attaaggagg agctatgtcg tcaaccctac gagaagccag taaggacacg ttgcaggcca
    25561 aagataaaac ttaccactac tacagcctgc cgcttgctgc taaatcactg ggcgatatca
    25621 cccgtctacc caagtcactc aaagttttgc tcgaaaacct gctgcgctgg caggatggta
    25681 actcagttac cgaagaggat atccacgcac tggcgggatg gctgaaaaat gcccatgctg
    25741 accgtgaaat tgcctaccgc ccggcaaggg tgctgatgca ggactttacc ggcgtacctg
    25801 ccgttgttga tctggcggca atgcgcgaag cggttaaacg cctcggtggc gatactgcaa
    25861 aggttaaccc gctctcaccg gtcgacctgg tcattgacca ctcggtgacc gtcgatcgtt
    25921 ttggtgatga tgaggcattt gaagaaaacg tacgcctgga aatggagcgc aaccacgaac
    25981 gttatgtgtt cctgaaatgg ggaaagcaag cgttcagtcg gtttagcgtc gtgccgccag
    26041 gcacaggcat ttgccatcag gttaacctcg aatatctcgg caaagcagtg tggagcgaat
    26101 tgcaggacgg tgaatggatt gcttatccgg atacactcgt tggtactgac tcgcacacca
    26161 ctatgatcaa cggccttggc gtgctggggt ggggcgtggg tgggatcgaa gcagaagccg
    26221 ccatgttagg ccagccggtt tccatgctta tcccggacgt agtgggcttt aaacttaccg
    26281 gaaaattacg tgaaggtatt accgccacag acctggttct cactgttacc caaatgctgc
    26341 gcaaacatgg cgtggtgggg aaattcgtcg aattttatgg tgatggtctg gattcactac
    26401 cgttggcgga tcgcgccacc attgccaata tgtcgccaga atatggtgcc acctgtggct
    26461 tcttcccaat cgatgcagta accctcgatt acatgcgttt aagcgggcgc agcgaagatc
    26521 aggtcgagtt ggtcgaaaaa tatgccaaag cgcagggcat gtggcgtaac ccgggcgatg
    26581 aaccgatttt taccagtatg ttagaactgg atatgaatga cgttgaagcg agcctggcag
    26641 ggccaaaacg cccacaggat cgcgttgcac tgcccgatgt accaaaagca tttgccgcca
    26701 gtaacgaact ggaagtgaat gccacgcata aagatcgcct gccagtcgat tatgttatga
    26761 acggacatca gtatcagtta cctgatggcg ctgtggtcat tgctgcgata acctcgtgca
    26821 ccaacacctc taacccaagt gtgctgatgg ccgcaggctt gctggcgaaa aaagccgtaa
    26881 ctcttggcct caagcggcaa ccgtgggtca aagcgtcgct ggcaccgggt tcgaaagtcg
    26941 tttctgatta tctggcaaaa gcgaaactga caccgtatct cgacgaactg gggtttaacc
    27001 ttgtgggata cggttgtacc acctgtattg gtaactctgg gccgctgccc gatcctatcg
    27061 aaacggcaat caaaaaaggc gatttaaccg tcggtgcggt gctgtccggc aaccgtaact
    27121 ttgaaggccg tatccatccg ctggttaaaa ctaactggct ggcctcgccg ccgctggtgg
    27181 ttgcctatgc gctggcggga aatatgaata tcaacctggc ttctgagcct atcggccatg
    27241 atcgcaaagg cgatccggtt tatctgaaag atatctggcc atcggcacaa gaaattgccc
    27301 gtgcggtaga acaagtctcc acagaaatgt tccgcaaaga gtacgcagaa gtttttgaag
    27361 gcacagcaga gtggaaggaa attaacgtca cacgatccga tacctacggt tggcaggagg
    27421 actcaaccta tattcgctta tcgcctttct ttgatgaaat gcaggcaaca ccagcaccag
    27481 tggaagatat tcacggtgcg cggatcctcg caatgctggg ggattcagtc accactgacc
    27541 atatctctcc ggcgggcagt attaagcccg acagcccagc gggtcgatat ctacaaggtc
    27601 ggggtgttga gcgaaaagac tttaactcct acggttcgcg gcgtggtaac catgaagtga
    27661 tgatgcgcgg caccttcgcc aatattcgca tccgtaatga aatggtgcct ggcgttgaag
    27721 gggggatgac gcggcattta cctgacagcg acgtagtctc tatttatgat gctgcgatgc
    27781 gctataagca ggagcaaacg ccgctggcgg tgattgccgg gaaagagtat ggatcaggct
    27841 caagccgtga ctgggcggca aaaggacctc gtctgcttgg aattcgtgtg gtgattgccg
    27901 aatcgtttga acgaattcac cgttcgaatt taattggcat gggcatcctg ccgctggaat
    27961 ttccgcaagg cgtgacgcgt aaaacgttag ggctaaccgg ggaagagaag attgatattg
    28021 gcgatctgca aaatctacaa cctggcgcga cggttccggt gacgcttacg cgcgcggatg
    28081 gtagccagga agtcgtaccc tgccgttgtc gtatcgacac cgcgacggag ttgacctact
    28141 accagaacga cggcattttg cattatgtca tccgtaatat gttgaagtaa caagtatttg
    28201 cttgccggtt attttgcttc cggcaagcaa atgaattaca caatgcaaga gggttatttg
    28261 ttcagcaaat ggcccatttt ctcggctttg gtatcgagat aatgttcgtt attggggtta
    28321 cgacctacaa tcaatggtac gcgttcaaca atattaatcc ctgcttcggt cagaatttcg
    28381 acttttttcg ggttattggt taacaagcgg acttcattga cgccaaggag tttgaacata
    28441 tcagcgcaaa gagtgaagtc gcgctcatca gcggcgaagc ctaactggtg gttagcctct
    28501 acggtatcgt aaccttgatc ctgcagtgcg taagcgcgga ttttattcag cagaccaatg
    28561 ttacgacctt cctgacggtg atacagcaaa ataccacggc cttcctcggc aatttgcgtc
    28621 aatgccgctt cgagctggaa gccacaatcg cagcgcaagc tgaacagggc gtcaccggtc
    28681 agacattcgg aatggacgcg cgcaagtacc ggggtatgcc cggaaatatc ggcatagact
    28741 agcgcgacat gatcgtgtcc ggttgccagt tcttcaaatc ccaccatcag gaaatcgccc
    28801 catggggttg gcagtttggc ttctgccaca cgtttaagct gcatgaaatt ctccagataa
    28861 tgctggttct gtattggctt attttgccat aacgagaagg ggttcaccta atcacgacgc
    28921 gtcgatcgtt cacggaatgg cacaaatctg tcaatttttc ttggaactgg cattttcagt
    28981 tatgattgtg ggacttatca aaaaggagag gccatgcgtt cgattgcccg acgtaccgca
    29041 gtgggagctg cactattgtt tgtcatgcca gtagccgtat ggatttctgg ctggcgttgg
    29101 caacctggag aacaaagttg gcgactaaaa gcggcttttt gggttactga aactgtcacc
    29161 cagccctggg gcgtcattac acatttgatt ttgttcggct ggtttctctg gtgtctgcgt
    29221 tttcgcatta aggctgcctt tgtattattt tccattctgg cggcggcaat ccttgttgga
    29281 caaggcgtta aatcctggat caaagacaaa gtacaggaac cacgaccttt tgttatctgg
    29341 ttggaaaaaa cacatcatat tccggttgat gagttctaca ctttaaagcg agcagaacgc
    29401 ggaaatctag taaaagaaca gttggctgaa gagaaaaata ttccacaata tttgcgttca
    29461 cactggcaga aagagacggg gtttgccttt ccttccggtc acacgatgtt tgctgccagt
    29521 tgggcactgc tggccgttgg tttgctgtgg ccgcgtcggc gaacgttaac cattgctatc
    29581 ttgttggtct gggcaacggg agtcatggga agccgcctgc tactcgggat gcattggcca
    29641 cgcgatctgg tggtagctac gttgatttcg tggacgctgg tggcggtggc aacgtggctt
    29701 gcgcaacgaa tttgtgggcc attaacacca cctgcggaag aaaatcgcga aattgcacaa
    29761 cgagaacaag aaagttaacg ctggttgatt ttccgaattt aggccttaaa tcatcaacaa
    29821 tgcgcgtgga tgccatttcg cagacggcgc gaaaatggta ctttaaaggg ctattgcggt
    29881 aagttgacca taatttattc gctctaacca cataacggga agtaatgtga aatatttact
    29941 cattttctta ctggtgttag cgatcttcgt gatttcggtc acgttgggtg cgcagaacga
    30001 tcaacaggtg acgtttaatt atctgttagc gcaaggggag taccgtattt ccacattgct
    30061 ggcggtattg tttgctgcgg ggtttgctat cggttggttg atttgtggcc tgttctggct
    30121 gcgagttcgt gtttccctgg cgcgcgctga acgtaaaata aagcgactgg aaaaccagct
    30181 ttcacccgcg actgacgtgg ctgtagtgcc gcactcgtca gcggcgaagg aataactttc
    30241 tatgctggag ttgttgtttc tgcttttgcc tgtagccgct gcctatggct ggtatatggg
    30301 ccgcagaagt gcgcaacaaa acaagcaaga tgaagccaac cgcttgtcgc gtgattacgt
    30361 agcgggggtt aacttcctgc ttagtaatca acaggataaa gcggtagacc tgtttctcga
    30421 tatgcttaaa gaggatacag gcaccgttga agcccacctt acgctcggaa acctgttccg
    30481 ttcgcgtggc gaagttgatc gcgctattcg catccatcag accctaatgg aaagcgcctc
    30541 gctgacctat gaacagcgtc tgttggcgat tcaacaactg gggcgtgatt acatggccgc
    30601 cgggttatac gaccgcgcgg aagacatgtt caatcagctg accgatgaaa ctgacttccg
    30661 cattggcgcg ctgcaacagt tgctacaaat ctaccaggct accagtgagt ggcagaaagc
    30721 aattgatgtt gccgaacgcc tggtgaagct gggtaaagat aaacagcgcg tcgaaattgc
    30781 ccatttctac tgtgagttag ccctgcagca tatggccagc gacgatctcg atcgtgccat
    30841 gaccttgcta aaaaaagggg cggcggcaga taaaaacagc gcccgcgtat ccatcatgat
    30901 gggacgcgtg tttatggcga aaggagaata cgccaaagcc gtcgaaagtt tgcaacgcgt
    30961 catatcccag gacagagaac tggtcagcga aacgctggaa atgttgcaaa cctgctatca
    31021 gcagttgggt aaaactgccg aatgggcaga attcctgcaa cgcgcggtgg aagagaacac
    31081 cggtgccgat gctgaattga tgcttgctga tatcatcgaa gcgcgcgacg gtagtgaggc
    31141 tgcacaggtc tatattacac gccagcttca gcgtcatccg accatgcgtg tattccataa
    31201 gctaatggat taccacttaa atgaagcgga agaagggcgc gccaaagaga gtctgatggt
    31261 gctgcgtgac atggttggcg agaaggtgcg tagtaagcct cgttatagct gccagaaatg
    31321 tggttttacc gcatacaccc tctactggca ttgtccgtct tgtcgtgcct ggtcaactat
    31381 taaaccgatt cgcggtcttg atggcctgta atttttaaaa aaatccgact ttagttacaa
    31441 catactagtt attgatgtcc cattgtgctc cggcaacgac ggagcagaaa aagcctgcta
    31501 ggggaacaat cgcaactgtt aattttttat ttccacgggt agaatgctcg ccgtttacct
    31561 gtttcgcgcc acttccggtg cccatcatca agaaggtctg gtcatgacgt taactgcttc
    31621 atcttcttcc cgcgctgtta cgaattctcc tgtggttgtt gcccttgatt atcataattg
    31681 tgatgacgcg ctgtcctttg tcgacaagat cgacccacgc gattgtcgtc tgaaggtcgg
    31741 taaagagatg tttacattgt ttgggccaca gtttgtgcgc gaacttcaac agcgtggttt
    31801 tgatatcttt cttgacctga aattccacga tatccccaac actgcagcgc acgctgtcgc
    31861 tgctgcagct gacttaggcg tgtggatggt gaatgctcat gcctccggtg gggcgcgtat
    31921 gatgaccgca gcgcgtgagg cactggttcc gtttggcaaa gatgcaccgc ttttgattgc
    31981 tgtgacagtg ttgaccagca tggaagccag cgacctggcc gatcttggcg tgacactgtc
    32041 acctgcagat tatgcagaac gtctggcagc actgacgcaa aaatgtggcc ttgatggtgt
    32101 ggtgtgttct gctcaggaag ctgtgcgctt taaacaggta ttcggtcagg agttcaaact
    32161 ggttacaccg ggcatccgtc cgcaggggag tgaagctggt gaccagcgcc gcattatgac
    32221 gccagaacag gcgttggcgg ctggtgttga ttatatggtg attggtcgcc cggtaacgca
    32281 atcggtagat ccagcgcaga cactgaaagc gatcaacgcc tctttacagc ggagtgcatg
    32341 atgagtgatt ccaacagccg tctggtctac tcaacggaaa ccgggcgtat tgatgagccc
    32401 aaagcggccc cagtacgccc taaaggcgat ggagcggtgc gtattcagcg tcagaccagt
    32461 ggacgtaaag gtaagggcgt ttgcctgatt accggtatcg atctcgatga tgccgaactg
    32521 acaaaacttg cagcggaact gaagaaaaaa tgcggctgcg gcggagcagt taaagatgga
    32581 gttattgaaa tccagggcga taagcgtgat ttattaaaat cactacttga agcgaaaggg
    32641 atgaaggtaa aactcgcagg cggttaacat aaaaagccac ggatatatcc gtggctttcg
    32701 aatattttac tgtgtgtatt attcagttat ttcgtactga acagaatcaa gcgtattata
    32761 attgcgagtc gataatcgac atttatttac gattatttac cgacctggtg accaataaca
    32821 ccaccgacag ctgcaccacc taatgtaccc aacgtactgc catcggtcag tactgcaccg
    32881 cctaatgccc ctgcacccgc gccgattgcg gtgttgcggt cccgtttaga ccagttagaa
    32941 caggcactca gagacattgc caaagtaatt gccagaacag ccgcggtcat ttttttgctc
    33001 gttacaaaca taatactctc tcctgaattt atgattcacg gaagtaagct ctctataact
    33061 ataatagcta agaataagcc tggtgaaatt attccgtgaa atctgctcgc gcgcagggtt
    33121 atatcacg
//
LOCUS       DADLTF010000029        33000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.029, whole genome shotgun sequence.
ACCESSION   DADLTF010000029 DADLTF010000000
VERSION     DADLTF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.029"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            16..4548
                     /locus_tag="JN749_001308"
     CDS             16..4548
                     /locus_tag="JN749_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7367778.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDVWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSS
                     SDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTP
                     DDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIA
                     DALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDKTANGGAGAYNAS
                     HDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEEN
                     GAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALG
                     SSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEA
                     HDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIG
                     LNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEF
                     SVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVT
                     NIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAE
                     EENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNIT
                     LGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQRMVEMDNKLSKTESKL
                     SGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGST
                     NSQGEYSAALGAGIQW"
     gene            4916..6571
                     /gene="lldP"
                     /locus_tag="JN749_001309"
     CDS             4916..6571
                     /gene="lldP"
                     /locus_tag="JN749_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HAY7367779.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6571..7347
                     /gene="lldR"
                     /locus_tag="JN749_001310"
     CDS             6571..7347
                     /gene="lldR"
                     /locus_tag="JN749_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HAY7367780.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7344..8534
                     /gene="lldD"
                     /locus_tag="JN749_001311"
     CDS             7344..8534
                     /gene="lldD"
                     /locus_tag="JN749_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HAY7367781.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8730..9203
                     /gene="trmL"
                     /locus_tag="JN749_001312"
     CDS             8730..9203
                     /gene="trmL"
                     /locus_tag="JN749_001312"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAY7367782.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="JN749_001313"
     CDS             complement(9256..10077)
                     /gene="cysE"
                     /locus_tag="JN749_001313"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAY7367783.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="JN749_001314"
     CDS             complement(10157..11176)
                     /gene="gpsA"
                     /locus_tag="JN749_001314"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAY7367784.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11176..11643)
                     /gene="secB"
                     /locus_tag="JN749_001315"
     CDS             complement(11176..11643)
                     /gene="secB"
                     /locus_tag="JN749_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HAY7367785.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="JN749_001316"
     CDS             complement(11706..11957)
                     /gene="grxC"
                     /locus_tag="JN749_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HAY7367786.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12099..12530)
                     /locus_tag="JN749_001317"
     CDS             complement(12099..12530)
                     /locus_tag="JN749_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAY7367787.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12775..14319
                     /gene="gpmM"
                     /locus_tag="JN749_001318"
                     /gene_synonym="pgmI"
     CDS             12775..14319
                     /gene="gpmM"
                     /locus_tag="JN749_001318"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HAY7367788.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14329..15612
                     /gene="envC"
                     /locus_tag="JN749_001319"
     CDS             14329..15612
                     /gene="envC"
                     /locus_tag="JN749_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HAY7367789.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15616..16575
                     /locus_tag="JN749_001320"
     CDS             15616..16575
                     /locus_tag="JN749_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HAY7367790.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="JN749_001321"
     CDS             complement(16562..17596)
                     /gene="waaH"
                     /locus_tag="JN749_001321"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HAY7367791.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="JN749_001322"
     CDS             complement(17835..18860)
                     /gene="tdh"
                     /locus_tag="JN749_001322"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAY7367792.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="JN749_001323"
     CDS             complement(18870..20066)
                     /gene="kbl"
                     /locus_tag="JN749_001323"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAY7367793.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="JN749_001324"
     CDS             complement(20341..21198)
                     /gene="yibB"
                     /locus_tag="JN749_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HAY7367794.1"
                     /translation="MKSSTTIITAYFDIGRGDWTTNKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKSYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21502..22434
                     /gene="rfaD"
                     /locus_tag="JN749_001325"
     CDS             21502..22434
                     /gene="rfaD"
                     /locus_tag="JN749_001325"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HAY7367795.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22444..23490
                     /gene="rfaF"
                     /locus_tag="JN749_001326"
     CDS             22444..23490
                     /gene="rfaF"
                     /locus_tag="JN749_001326"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HAY7367796.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23494..24486
                     /gene="rfaC"
                     /locus_tag="JN749_001327"
     CDS             23494..24486
                     /gene="rfaC"
                     /locus_tag="JN749_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HAY7367797.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24483..25691
                     /gene="rfaL"
                     /locus_tag="JN749_001328"
     CDS             24483..25691
                     /gene="rfaL"
                     /locus_tag="JN749_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HAY7367798.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25727..26869)
                     /locus_tag="JN749_001329"
     CDS             complement(25727..26869)
                     /locus_tag="JN749_001329"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HAY7367799.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26878..27891)
                     /locus_tag="JN749_001330"
     CDS             complement(26878..27891)
                     /locus_tag="JN749_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HAY7367800.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="JN749_001331"
     CDS             complement(27916..28623)
                     /gene="rfaY"
                     /locus_tag="JN749_001331"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HAY7367801.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="JN749_001332"
                     /gene_synonym="rfaI"
     CDS             complement(28649..29656)
                     /gene="waaO"
                     /locus_tag="JN749_001332"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HAY7367802.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="JN749_001333"
     CDS             complement(29699..30496)
                     /gene="rfaP"
                     /locus_tag="JN749_001333"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HAY7367803.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(30489..31613)
                     /locus_tag="JN749_001334"
     CDS             complement(30489..31613)
                     /locus_tag="JN749_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAY7367804.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="JN749_001335"
     CDS             complement(31610..32668)
                     /gene="rfaQ"
                     /locus_tag="JN749_001335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HAY7367805.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         8729 a         8069 c         7787 g         8415 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cgtctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaatcaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     1921 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     1981 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2041 ggcggcaatg cttctgtgaa tactgacggc gtgtttaccg gtccaaccta caaaatcggt
     2101 gaaacaaatt attataacgt cggcgatgct ctgctttggg atgccaccgc aggtaaattc
     2161 agtgccaaac acggtactaa tggtgacgca agcgtgatca ctgatgtcgc agatggtgaa
     2221 atttcagact ccagttctga cgcagtaaac ggctcacaac tccacggcgt gagcagttat
     2281 gttgttgatg cgctgggggg tggtgccgaa gtcaatgcag acggcaccat cactgcgccg
     2341 acgtacacca ttgctaatgc tgattacgat aatgtcggtg atgccctgaa tgctatcgat
     2401 accactcctg acgacgctct gctctgggat gcggacgccg gtgaaaatgg tgcatttagc
     2461 gccgctcacg gaaaagataa aactgccagt gtaatcacta acgtcgctaa cggtgcaatc
     2521 tctgctgcca gcagcgacgc gattaacggc tcacaactct ataccaccaa taagtacatc
     2581 gctgatgcgc tgggtggtga cgcagaagtc aacgctgacg gcaccatcac cgcaccgact
     2641 tacaccattg cgaacgccga gtacaacaac gtcggtgacg ccctggatgc gcttgatgat
     2701 aacgccctgc tgtgggataa gactgccaat ggcggtgctg gagcctacaa tgccagccat
     2761 gacggtaaag ccagcatcat cactaatgtc gctaatggca gtattagtga ggacagtacc
     2821 gatgcagtga acggttctca gttgaatgcg acgaatatga tgattgagca gaacacccaa
     2881 attatcaatc agctcgctgg taacaccgac gcaacctata tcgaagaaaa tggcgctggt
     2941 atcaactacg ttcgtactaa cgacaacgat ttagccttta acgatgcaag cgcctctggt
     3001 gtcggcgcta cagctgtagg ttataacgct gtcgcgtctg gtgccagcag cgtagccatt
     3061 ggtcagaaca gcagcagcac cgttgatacc ggtattgcgc tgggtagcag ctccgtttcc
     3121 agccgtgtga ttgccaaagg ttctcgtgac actagcgtaa cggaaaatgg cttggtgatt
     3181 ggttatgaca ccactgacgg cgaactgcta ggtgcattgt caattggtga tgacggtaaa
     3241 taccgccaaa tcatcaacgt agctgatggt tcagaagccc atgacgccgt tacggttcgc
     3301 cagttgcaga acgctattgg agcggtcgcc actacgccaa ccaagtactt tcacgccaac
     3361 tcaacggcag aagactcact ggccgttggt gaagactcgc tggcaatggg tgcgaaaact
     3421 gtcgttaatg gtaatgcagg tattggtatc ggtttgaaca ctctggttct ggctgatgcg
     3481 atcaacggca ttgctatcgg cagtaacgca cgcgcaaacc atgcaaacag tatcgcaatg
     3541 ggtaacggtt ctcagaccac ccgtggtgca cagactggct acaccgccta caacatggac
     3601 gcaccgcaga actctgtagg tgagttttct gtcggtagtg aagacggtca acgtcagatc
     3661 acaaacgtcg cagctggttc agcggatacc gatgcggtta acgtgggtca gttgaaagtc
     3721 actgatgagc gcgtagcgca aaatacccag agcattacta acctgaacaa tcaggtcact
     3781 aatctggata ctcgcgttac taatatcgaa aacggtattg gcgacattgt caccaccggt
     3841 agcaccaagt acttcaagac caacaccgat ggcgtagatg ccaacgccca gggtaaagat
     3901 agcgttgcta ttggttctgg ttccattgct gccgctgaca acagcgtcgc actgggtacc
     3961 ggttccgttg cagaggaaga aaatacaatc tctgtaggtt cttctactaa ccaacgccgt
     4021 atcaccaacg tagctgccag tgttaatgcc accgatgcgg ttaacgtgtc gcagctgaaa
     4081 tcttctgaag caggcggagt gcgctacgac accaaagctg atggttctat cgactatagc
     4141 aatatcaccc tcggtggtgg caacggcagt acgactcgta tcagcaacgt ctccgctggc
     4201 gtcaacaaca acgacgcggt gaactacgcg cagttgaagc aaagcgcgca agaaacgaag
     4261 caatacaccg atcagcggat ggttgagatg gataacaaac tgtctaaaac tgaaagcaag
     4321 ttgagcggtg gtatcgcttc tgcaatggca atgaccggtc tgccgcaggc ttatacaccg
     4381 ggtgccagca tggcttctat tggtggcggt acttacaacg gtgaatcggc agttgcttta
     4441 ggtgtatcga tggtgagcgc caatggtcgt tgggtctaca aattacaagg tagtaccaat
     4501 agccagggtg aatactccgc cgcactcggt gccggtattc agtggtaatg atccattaag
     4561 ttagtgtgac taagggcaaa cgccaaattg cctgatgcgt tacgcgcatc aggcaagata
     4621 aggcgctccg ggtcaacaat ctaagggcaa ttctctgatg aggattgccc ttttctttac
     4681 cagacatctc cccccacaag aattggccct accaattctt cgcttatctg acctctggtt
     4741 cacaattccc caattaaaac tcacatcaat attgataata cataacattt agttaaccat
     4801 tcattgtcat tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca
     4861 aggagatgag caacagactc attacacgat gtgcgtggac tctaggagac ctgcaatgaa
     4921 tctctggcaa caaaactacg atcccgccgg gaatatctgg ctttccagtc tgatagcatc
     4981 gcttcccatc ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc
     5041 cgcctcatgg acggtagcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt
     5101 cgctaacgcg ctggcctcgg tggtttatgg cttcttctat ggtttgtggc ccatcgcgtg
     5161 gatcattatt gcagcagtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat
     5221 cattcgctcg tctattcttt cgataacccc tgaccagcgt ctgcaaatgc tgatcgtcgg
     5281 tttctgtttc ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac
     5341 cgccgcattg ctggtcggcc tgggttttaa accgctgtac gccgccggac tgtgcctgat
     5401 tgttaacacc gcgccagtgg catttggcgc gatgggcatt ccaatcctgg ttgccggaca
     5461 agtaacaggc atcgacagct ttgagattgg ccagatggtg gggcgtcagc taccgtttat
     5521 gaccattatc gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaaaga
     5581 gacgtggcct gcggtcgtgg ttgcaggcgg ctcgtttgcc atcgctcagt accttagctc
     5641 taacttcatt gggccggagc tgccggacat tatctcttcg ctggtatcac tgctctgcct
     5701 gacgctgttc ctcaaacgct ggcaaccagt tcgcgtattc cgcttcggtg acttgggggc
     5761 gtcacaggtt gatataacgc tggcccacac cggttacacc gcgggtcagg tgttacgtgc
     5821 ctggacaccg ttcctgttcc tgaccgctac cgtaacactg tggagcatcc cgccgtttaa
     5881 agccctgttc gcatcgggtg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta
     5941 cctcaataaa ctggttgccc gtatgccacc agtggtcagc gaggctacag cctatgccgc
     6001 cgtgtttaag tttgactggt tctctgccac cggcaccgcc attctgtttg ctgcactgct
     6061 ctcgattgtc tggctgaaga tgaaaccgtc tgacgctatc agcaccttcg gcagcacgct
     6121 gaaagaacta gctctaccca tctactccat cggcatggtg ctggcgttcg cctttatctc
     6181 gaactattcc ggactgtcat caacactggc actggcactg gcgcacaccg gtcatgcatt
     6241 caccttcttc tcgccgttcc tcggctggct gggggtattc ctgaccgggt cggatacctc
     6301 atctaacgcc ctgttcgccg cgctgcaagc caccgcagca caacaaattg gcgtctctga
     6361 tctgttgctg gttgccgcca ataccaccgg tggcgtcacc ggtaagatga tctccccgca
     6421 atctatcgct atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg
     6481 ctttactgtc aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca
     6541 ggcttatgtc ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac
     6601 gaggttgccg atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag
     6661 ttgcccgctg agcgccaact ggcgatgcaa ctcggcgtat cacgtaattc actacgcgag
     6721 gcgctggcaa aactggtgag cgaaggcgtg ctgctcagtc gacgcggcgg cgggacgttt
     6781 attcgctggc gtcatgacac atggtcggag caaaacatcg tccagccgct aaaaacgctg
     6841 atggccgatg atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc
     6901 agcaccgcat ggcatgcggc aatgcgcgcc acacctggcg acaaagaaaa gattcagctt
     6961 tgctttgaag caacgctaag tgaagacccg gatatcgcct cacaagcgga cgttcgtttt
     7021 catctggcga ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc
     7081 ttcgatgtcc tgcaatcctc agtgaagcat agccgtcagc ggatgtatct ggtgccaccg
     7141 gttttttcac aactgaccga acaacatcag gctgtcattg acgccatttt tgctggtgat
     7201 gccgacgggg cgcgtaaagc aatgatggcg caccttagtt ttgttcacac caccatgaaa
     7261 cgattcgatg aagatcaggc tcgccacgca cggattaccc gcctgcccgg tgagcataat
     7321 gagcattcga gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag
     7381 cgcaacgcat tctgccgccg ttcctgttcc actatatgga tggtggtgca tattctgaat
     7441 acacgctgcg ccgcaacgtg gaagatttgt cagaagtggc actgcgccag cgtattctga
     7501 aaaacatgtc cgacttaagc ctggaaacga cgctgtttaa tgagaaattg tcgatgccgg
     7561 tagcactggc accggtgggt ttgtgtggca tgtatgcgcg tcgtggcgaa gttcaggcag
     7621 ccaaagcggc ggacgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga
     7681 ttgaagaagt cgcgccagcc atcaagcgcc caatgtggtt ccagctttat gtactgcgcg
     7741 atcgcggctt tatgcgtaac gcgctggagc gagcaaaagc agcgggttgt tcgacgctgg
     7801 ttttcaccgt ggatatgccg acaccgggcg cacgctaccg tgatgcgcat tcaggtatga
     7861 gcggcccaaa tgcggcaatg cgccgctact tgcaggcggt gacgcatccg caatgggcgt
     7921 gggatgtggg cctgaacggt cgtccacatg atttaggtaa tatctcagct tatctcggca
     7981 aaccgaccgg actggaagat tacatcggct ggctggggaa taacttcgat ccgtccatct
     8041 catggaaaga ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga
     8101 tcctcgatcc ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt
     8161 ctaaccacgg tggccgccag ctggacggtg tactctcttc cgctcgtgcg ttgcccgcta
     8221 ttgcagatgc ggtgaaaggt gatatcgcca ttctggcgga tagcggaatt cgtaacgggc
     8281 ttgatgtcgt gcgtatgatt gcgctcggtg ccgacaccgt actgctgggt cgtgctttcc
     8341 tgtatgcgct ggcaacagcg ggccaggcgg gtgtagctaa cctgctaaat ctgatcgaaa
     8401 aagagatgaa agtggcgatg acgctgactg gcgcgaaatc gatcagtgaa attacgcaag
     8461 attcgctggt gcaggggctg ggtaaagagt tgcctacggc actggctccg atggcgaaag
     8521 ggaatgcggc atagtcgttt gcgccccctc accctaaccc tctccctcag ggagagggga
     8581 ccgttcggcg ctgtatgtac tccctcactc tgaaacgaca ccgcactctt tttttctccc
     8641 tcgcccctcc ggggagggcc ggggtgaggg gaaaaggccg cacggttcac caattttctg
     8701 ctatcctgcc cccgcactaa gggggcagta tgctaaacat cgtactttac gaaccagaaa
     8761 ttccgccaaa tactggcaac atcatccgtc tttgcgctaa taccggcttt cgtctacata
     8821 tcatcgaacc gatgggattt gcctgggacg acaagcgcct gcgccgcgcg gggctggact
     8881 atcacgagtt taccgccgtt acgcgtcatc atgactatcg cgcgttcctc gaagcagaaa
     8941 atccccagcg cctgtttgcc ctcaccacga aaggtacgcc tgctcacagc gccgtaagct
     9001 atcaggatgg cgattatctg atgttcggcc cggaaacacg cggcctgcca gcgagcattc
     9061 ttgatgccct gcccgctgaa caaaaaattc gcattccgat ggtgccggac agccgcagca
     9121 tgaatctgtc caatgcggtg tcggtagtgg tgtatgaagc ctggcggcag ctgggatatc
     9181 cgggagcgtt gttgagagat tagttactgt atgccggata gatgattaca tcgcatccgg
     9241 cacgatcaca ggacattaga tcccatcccc atactcaaat gtatggttaa taccgttgaa
     9301 atgctggtcc atatccattg atggcttatc gctgtctggt ttaccgacaa tacgagccgg
     9361 aacgccagcg gcggttgtat gcggcggcac cggttgcagc accacggaac ctgcgccaat
     9421 cttcgcgccg cgaccaactt caatattgcc gaggattttc gcgcccgcgc caatcatcac
     9481 accttcacga attttcgggt gacggtcacc accagattta cccgtaccgc caagcgtcac
     9541 agattgcaga atcgatacgt cgttttcaat taccgccgtt tcacccacaa cgatgcccgt
     9601 ggcgtggtcg agcataatgc cacggccaat ttttgctgcc gggtgaatat cgacctggaa
     9661 cgtcacagaa acctggtttt gcagaaagat tgccaatgcg cgacgcccct gattccacaa
     9721 ccagtgaccg atgcgatagg cctgcaaggc atgaaaaccc ttcaggtata acaacggggt
     9781 tgagtattta tcgactgccg ggtcgcgggt acgcaccgcc tgaatatcac aggccgcaga
     9841 ggcgatcatt tccgggtcag cggcgtaggc ttcttcaacc acttcacgga tagcaatagc
     9901 aggcataatt ggcgatgaca gcttgttcgc cagcatgtag ctcagtgcac tgccaaggtt
     9961 ttcgtgcttg agtagcgtcg cgtggtaaaa actggccagc attggctcac agtccgccag
    10021 cgttctggct tcggctttaa tattgttcca gacaatttcc agttcttcac acgacattgc
    10081 ttactccaca cgatgagata atgaccggcc cgttctgcgc gggccgggtc atagcggtaa
    10141 caaaggttcc ctggggttag tggctgctgc gctcgtcctt gcgtgcacga cctaataatg
    10201 tcaatgctgc ctcgcgcgcg ttttttccgc aatataatac ttgataaatt tcctcggtta
    10261 ttggcatttc aacgccgaag cgatgcgcca gttcgcggac ttctttcgta ttgcggtagc
    10321 cttccaccac ctgaccaatc ttctcctgcg cgctttgtac atccatgccc tgaccgagca
    10381 tcatgccaaa acggcggtta cgcgactggt tgtcggtaca ggtaagcacc agatcgccca
    10441 gccccgccat gcccataaag gtggcaggat cggcacccag cgccgcacca agacgcgaca
    10501 tttcagccag cccacgggtg atcagcgccg tacgcgcatt cgcaccaaaa ccgataccgt
    10561 cagacatccc cgcgccaatg gcaatgacgt ttttcaccgc gccgccaagc tgcacgccaa
    10621 tgaaatccgg gttgctgtaa acgcggaaac ttttgccgca gtgcagcagc tgctggagat
    10681 catcggcaaa ggtctgatcg gtcgaggcca gcgaaatagc tgtcggtaaa cctgccgcca
    10741 gttctttcgc aaacgttggg ccagagataa ccgccagcgg aatttgatcg cctaaggcct
    10801 cacgcgccac gtcctgtaac agacgtccgg tttccgcttc cagccctttg gtcgcccaca
    10861 ccagacgcgc atcaggacgc atcagcggtt taatctggcg cagcacttca ccaaagacat
    10921 ggctgggtac gacgacgaga atattacgac tggctgccag cgcagtggcg agatcgcttt
    10981 caagatggag cgtatcggga aaaggcacat cggggagaaa cgcggcgtta cagcggtcgc
    11041 gttcaagcgt tgcgatatgt tcagggtcat ggccccagag gacaacctcg tggccatttc
    11101 ttgccagggt aatggcaaga gcggtgccgt acgagccggc accgatcaca gtcattgaag
    11161 cattacgttg gttcatcagg catcctgatg ttcttcagta ccttcgccag cctgctgctg
    11221 caaatagttc atgaacagcg catcgaagtt aaccggcgca aggttcagtt gcgggaatgt
    11281 accgcgggat accatgctgg tgatgcactc acgagcatac gggaacagaa tgttcgggca
    11341 gtatgcgccc aggcaatgtg ccatctgggt gccttcgata cccgcgatgg agaaaatacc
    11401 gccctgctga acttcacaca ggaacgcggt ttcttcgccc aaggaggccg ttacggtaac
    11461 acgcagtacc acttcgtata cgtcatctgc cagttgggaa gatgccgtat ccagatcaag
    11521 tttaacttct ggttgccaat ctttctggaa aacgtgcggc gcgttcggcg cttcgaaaga
    11581 gatatccttg gtataaatac gttggatctg gaaagtcatt tcagtgttgt tttgttctga
    11641 catgtgtaga aaacccttaa gtgttgtcct taaatactgc gcaatgtaat gccaacgttc
    11701 acacgttatt tcagcagggg atccagtcca ccacgtgcat ccaatgcata caagtcatca
    11761 cagccgccaa tgtgctgtgc gtcaataaaa atctggggaa ccgtggtgcg accgctgcgt
    11821 ttgatcatct cttcacgctt ggcggcattg ccatcgatcg gcaactcctg gaaactcacg
    11881 cccttgctgc tcagcagtgc ttttgcacga tggcaatacg ggcaggtttc tttggtatag
    11941 atttcaacat tggccataat ttagctcctg tttttttacc ctgaaatttc atgttgcagg
    12001 gcggctgcaa aatggagtgt ccccagaagc ttataacaat aagtcgctgg gggcctaatt
    12061 gcagctaacg gcctgcatca tgaaagacga caggtaaatt atttgccgcg caccagaggc
    12121 aagttttcgc cagcccagcc agcaacgccc tctttcagca cgaatacttg cgcaaaacca
    12181 gctttcgtca gcgcgtttgc aggctcctgg cactgcatgc cagaaccgtc taccacgata
    12241 accgatttgt ctttgtgctt ctcaagctca ccaacattgt tggctttgat ttcgctcggc
    12301 aacagattga tagaacctgc gatatggcct ttacggaagt catcacgctg acgtaaatcc
    12361 acaaccacag cgtcttcttt gttgatcaga cgcgtagctt caccacgagt aatcaccttc
    12421 actttcgagg tcaggctctt aaacgtagtc acaagaaccg ccaccagtaa cgcgatccag
    12481 gcgatactca gtatgggatg acggccaaca aattgcataa tttcttgcat ggggggtaac
    12541 aactcccaac gtagtgatta aaaaaaccag gaaaggagta tacctgcgcg gtgcggcaaa
    12601 tacagccagc gcgttaactg gaatgcaatt ttgcggggca cgacgaaaaa aaagcgcaaa
    12661 tctggcatcg gcttgccgcg agcggtcgta tattttgatc ttcagaggct attttatcga
    12721 ttcagctgta gtaaaattac gcaaattttg actcttgagt atgaggttgt cgcaatgtcg
    12781 gtttctaaaa aacctatggt actggtgatt ctggatggct atggctatcg tgaagaacag
    12841 caggataacg ccatttttag tgctaaaacc ccggtaatgg atgcactgtg ggccaatcgt
    12901 ccgcataccc tgatcgacgc ttccggtctg gaagtcggtc tgcctgaccg tcagatgggt
    12961 aactccgaag taggccacgt taacctgggt gccggccgca tcgtgtatca ggacctgact
    13021 cgtctggacg ttgaaatcaa agatcgcgct ttctttgcta atccagtgct ggctggcgcg
    13081 gtacataaag cgaaaaacgc aggtaaagca gtacacatta tgggtctgct ctccgcaggc
    13141 ggcgtacaca gccacgaaga tcacatcatg gcgatggtag aactggcagc tgaacgcggc
    13201 gcagaaaaaa tctacctgca cgcattcctt gacggtcgcg acaccccgcc gcgcagtgct
    13261 gaatcctcgc tgaaaaaatt tgaagagaaa tttgctgcgc tgggtaaagg ccgcgtagcg
    13321 tccatcattg gtcgttacta cgcgatggac cgcgataacc gttgggatcg cgttgaaaaa
    13381 gcctatgacc tgctgactct ggcgcagggt gagttccagg ccgatactgc cgttgctggt
    13441 ttgcaggcag cttatgctcg cgacgaaaac gacgagttcg tgaaagcgac cgttattcgt
    13501 gcagaaggtc agccagatgc ggcaatggaa gacggcgatg cgctgatttt catgaacttc
    13561 cgtgctgacc gcgcgcgtga aatcactcgt gcattcgtga acgctgattt cgatggcttc
    13621 gcgcgtaaga aagtggttaa cgtcgatttc gtgatgctga ccgaatacgc cgctgacatc
    13681 aaaactgcgg ttgcttaccc acccgcttcc ctggttaaca ccttcggcga gtggatggcg
    13741 aaaaacgaca aaactcagtt gcgtatttcc gaaaccgaaa aatatgccca cgttactttc
    13801 ttcttcaacg gtggcgtaga agagtcgttc aaaggcgaag atcgcattct gatcaactca
    13861 ccgaaagtgg ctacctacga tctgcaaccg gaaatgagct ccgcagagct gaccgaaaaa
    13921 ctggttgcgg ccatcaagag cggcaaatac gacaccatta tctgtaacta tccgaacggc
    13981 gacatggtag gtcacaccgg ggtgatggaa gcggcggtta aagcggttga agcactggat
    14041 cactgcgtgg aagaagtcgc gaaagcggtt gagtccgtgg gtggacaact gctgatcacc
    14101 gctgaccacg gtaacgctga acagatgcgc gatccagcaa cgggtcaggc acacacggca
    14161 cacaccaacc tgccagttcc gctgatttac gttggtgata agaacgtgaa agcggttgca
    14221 ggcggcaaac tttctgacat cgcgccgacc atgttgtcgc tgatgggtat ggaaatcccg
    14281 caagagatga ctggtaagcc gctgttcatc gtggaataat ccctccccat gaggggaaag
    14341 gcgattaata ccatgacacg ggccgtgaaa ccgcgcaggt ttgcaatcag gcccatcatc
    14401 tacgccagcg ttcttagcgc tggcgtattg ttgtgcgcct tttccgccca cgcggatgag
    14461 cgtgaccaac tcaaatctat tcaggccgat atcgccgcaa aagagcacgc ggtacgccaa
    14521 aagcaacaac aacgcgcaag cctgctcgca caattgaaaa agcaggaaga agcgatctct
    14581 gaagccaccc gtaagctgcg cgaaacgcaa aacacgctca atcaactcaa taaacagatt
    14641 gatgagatga acgcgtcgat tgccaaactg gagcagcaaa aagccgccca ggagcgcagc
    14701 ctcgccgcac aactggatgc cgcattccgt cagggcgagc ataccggtat tcagctaatt
    14761 ctcagcggtg aagaaagcca gcgtggacag cgtttacagg cttatttcgg ctatctcaac
    14821 caggcgcgac aagaaaccat tgcccagttg aagcaaacgc gtgaagaagt caccatgcag
    14881 cgtgctgaac tggaagagaa acaaagcgag caacaaacgc tgttatatga acagcgcgcc
    14941 caacaggcga aactgactca ggttctgaac gagcgtaaaa agacgctggc ggggctggaa
    15001 tcttccatcc agcaaggtca gcagcagcta agtgaactac gtgccaacga atcccgtctg
    15061 cgtaacagca ttgcccgtgc ggaagccgcg gcgaaagcgc gtgctgaacg agaagcgcgt
    15121 gaggcccagg cggttcgcga gcgccagaaa gaagcgacgc gcaaaggcac cacctacaaa
    15181 ccgaccgaaa gcgaaaaatc gctgatgtcc cgaactggtg gcctgggtgc gccgcgtggt
    15241 caggcattct ggccggttcg cgggccgacg ctgcatcgct atggcgagca gctacagggt
    15301 gaattacgct ggaaagggat ggttatcggt gcctctgaag gtactgaagt taaagcaatt
    15361 gccgacggtc gagtgattct ggctgactgg ctgcaaggtt acggtctggt ggtggtggtt
    15421 gagcacggta aaggcgacat gagtctttac ggctataatc agagcgcact ggtgagcgtt
    15481 ggttcgcagg ttcgcgcggg ccagccaatt gcactggtgg gcagcagtgg cggtcagggt
    15541 cggccttcac tctatttcga aattcgccgc cagggtcagg cggtcaatcc acagccgtgg
    15601 ttgggaagat aagttttgtt tccatttcgt cgtaacgttc ttgcatttgc cgctctgttg
    15661 gcgctctcct cccccgtact tgctggcaaa cttgccatcg tcattgatga ttttgggtat
    15721 cgcccgcaca acgaaaacca ggtgctggcg atgccttccg ctatctccgt cgctgtatta
    15781 cccgattcac cgcacgccag agagatggcg accaaagcgc ataacagcgg tcacgaagtg
    15841 ttgattcatc tcccgatggc accgttgagt aaacagccgc tggagaaaaa tacgctacgc
    15901 ccggagatga gcagcgacga aattgagcgc attattcgta gtgcggtcaa taacgtgccc
    15961 tatgccgtgg ggatcaacaa ccacatgggc agcaagatga cctctaacct gtttggtatg
    16021 cagaaagtga tgcaggcgct ggagcgttac aatctttact tcctcgacag cgtaaccatc
    16081 ggtaataccc aggcgatgcg cgccgcgcaa ggtactggcg tgaaggtgat caaacggaag
    16141 gtgttcctcg acgattcgca gaacgaagcg gacatccgtg tgcaatttaa tcgcgcaatt
    16201 gatctggcgc gtcgcaacgg ttcaaccatt gccattgggc atcctcaccc ttcaacggta
    16261 cgcgtgctac aacagatggt ttataacctg ccgccagaca ttacgctggt gaaagccagc
    16321 agcttgctga atgaaccgca ggttgatact tctacaccgc cgaaaaacgc tgtgcctgat
    16381 gcaccgcgta atccgttccg tggcgtgaag ctgtgcaaac cgaagaaacc gatagaacct
    16441 gtttatgcta atcgcttctt tgaagtatta agcgaaagca tcagccagag cacgctgatc
    16501 gtttacttcc agcatcagtg gcaaggctgg ggcaaacagc acgaagcggc gaagtttaac
    16561 gctagcgcaa attaaaggcg ctacgcgtaa tgcgatggtg cgaccgcgtt ttgtcgcgcc
    16621 attgccataa gcggaaagac cacagtaacg cctggtaacc gaccttcacg ctgcgcacat
    16681 tggtaatcag gcgcttatac ataccggaag taaatatctc ggcaatcatc cgttggcggg
    16741 taagaatatc cggctctttg cgcaccgcat ggcaaacacg caatgcttcg taagttattt
    16801 gctgatgaaa ttccggataa atcgtaattt tgtcggcata atttcgattt aatttctcca
    16861 gcaggcgggt aatcttaata tagtgacgtt gataattaag gtttttattc ccttgtctat
    16921 gcaaccgact cactgacgta ttatgcagat aatatttata taatgattgc tcggtatatc
    16981 gcgcacgcag cgcgttaaac atgaattctg ttgtccagac aatatcctga tgatgtaatc
    17041 cggcaataaa tttaatgtta tttttaacaa taacatcacg gcgataaacc cccatccaga
    17101 caacgtgagt ccaacggcgc gaagaaagcc ccatccgcag ccagtccggg ccggttaata
    17161 caccggttga gcgaaggcga tcgctgggga tggattgcca ggtttctccc gtttcacgaa
    17221 aagaccagtc agcgttgcac tgcgccacgt cgaggtcgtc ctctaacgcc atggtcatca
    17281 gcgtttcgta catggtggga tagacctcat cgtcagcatc gacaaaagcg acatatttgc
    17341 ccgtcgccac ctcgatccca cgattacgcg ctaccgatgc gccagcattc gcctgatgca
    17401 acaaacgaac gtgcggatag ttttctgcgt aatgctttgc tatttcaaca gaattatccg
    17461 ttgaaccatc gttaataata atgatttcca gagcagtcca ggtttgcgta attaaagatt
    17521 ccatacaagt gcggaaatca tcgcccgcat tatataacgg aataataaca ctaagtttat
    17581 tggtgctgtt catcataaat taccgacgac ctctgaagtt atcctgacgt tttacaggct
    17641 ttgtattaag aaactattaa gcctgtgaga tattattttt acaggtgaga gagtcacttc
    17701 agggtagaga gataatgaga acagctatat aaaagatcag cgcaattacg aactctttat
    17761 tacactgaca ggattttaca taattaacag attatcctca gataaaaggg ctggaatacc
    17821 agcccttgtt cgtgttaatc ccagctcaga ataactttcc cggactggcc cgaacgcata
    17881 gcgtcaaagc ccttctggaa atcatcgata gagaaacgat gggtaatgat cggcgagaga
    17941 tcgaggccag actgaatcag cgccgccatc ttgtaccaag tttcaaacat ctcacgaccg
    18001 taaatgcctt taatgaacaa gcctttaaag atcactttgg tccagtcgat agacatatca
    18061 gacggtggaa tacccagcat cgcaatacga ccgccgtggt tcatggtatc gagcatggta
    18121 cgaaacgctg gcggcgcgcc ggacatttcc agaccgacat caaagccttc ggtcatgccc
    18181 agttcagcca tcacatcatt aaggttttct ttcgcgacgt taaccgcacg ggtgataccc
    18241 attttgcgcg ccagctcaag gcggtattcg ttaacatcgg tgatcaccac attgcgtgca
    18301 ccaacgtgtt tcgccaccgc cgcagccata atacctatcg gacctgcacc agaaaccagc
    18361 acatcttcgc ccaccagatc gaacgacagc gcggtatgca cggcgttacc gaagggatca
    18421 aaaatggaag ccaggtcgtc ggagatattg tcggggattt tgaaagcgtt gaacgccgga
    18481 atcaccagat attcggcgaa gcagcccgga cggttaacac cgacaccaat ggtgttgcgg
    18541 cacagatggg tacgaccgcc acgacagttg cgacagtgac cgcaggtgat atggccttcg
    18601 ccagaaacgc gatcgccgat tttgaagcct ttcacttcct gaccaatacc taccacttca
    18661 ccgacatatt catggcccac gaccatcggc accggaatgg ttttttgcga ccactcatcc
    18721 cagttataga tgtgaacgtc agtcccgcag atggctgttt tacggatttt aatcagcaga
    18781 tcgttatgcc cgagttccgg tacagggacg tcggtcatcc agatgccctc ttccgctttc
    18841 agtttggata acgctttcat ctcacatcct caggcgataa cgcccagttg tttaccaata
    18901 cgcgtgaatg cttctactgc acgcgtaatt tgctcagggg tatgcgccgc agacatctgg
    18961 gtacgaatac gcgcctgacc tttcggaacg accggataga agaaaccggt gacgtagatg
    19021 ccctcttttt gcagctctcg ggcaaatttc tgcgccacta ccgcatcacc aagcatgacc
    19081 ggaataatgg cgtgatcggc tcccgccagg gtaaagcccg ccgccgacat ttgctcacgg
    19141 aactgacgcg cgttcgccca cagacggtca cgcaactcgc tacccgcttc aaccatctcc
    19201 agcactttga tggaagcggc gacaatcgct ggtgccagcg agttggagaa caggtacgga
    19261 cgagaacgct ggcgcagcca ctcaaccact tctttgcgcg ccgcggtata accaccagaa
    19321 gccccgccca gcgctttacc gagcgtgccg gtgatgatgt cgacacgccc catcacatcg
    19381 cagtattcat gggaaccacg accattttca ccgacaaaac cgaccgcgtg ggagtcgtct
    19441 accatcacca gggcatcgta tttatctgcc agatcgcaaa cgcccttcag attggcaatc
    19501 acgccgtcca tcgagaacac accatcggtg gcaatcagca catgacgcgc accggcttca
    19561 cgcgcttctt tcagacgagc ttcaagctcc tgcatatcgt tgttggcata gcgatagcgt
    19621 ttagctttac acagacgcac accatcaata atagacgcgt ggttcagtgc gtcggagata
    19681 atggcgtctt ccgcgcccag cagcgtttca aacaggccac cgttagcatc aaagcaggaa
    19741 gagtagagaa tcgcatcttc catccccagg aatgccgcca gtttttgttc aagctcttta
    19801 tggctgtcct gagtgccgca aataaaacgc accgaagcca tgccgaaacc gtgagaatcc
    19861 attcccgcct ttgctgccgc aatcagatcc ggatgattcg ccagcccgag atagttgttg
    19921 gcacaaaagt taatgacgtg gcttccatca gccacggtga tatctgcttg ctgcgcagac
    19981 gtaataatgc gctcttcttt aaacaaccct tccgcccgtg cggtttccag atcgttggtt
    20041 aactgctgat aaaattctcc acgcattgcg attctccaga cagggcaaat tccagcacat
    20101 attacccaaa cttataggtc gcgacgagat gatgcgttaa cacttctgca aaattcagga
    20161 taaataacgt gtatcttgat gtctctcaac acctacagat agtagaaaaa ctgtacagtt
    20221 ttggctgagc ataatgatga gtgattttac tgcattgata taaaaagaaa aaatgatgcc
    20281 aaagcatcat ttttcctatc tcagttggca tcaaaacgct acccactaat ggagggaaac
    20341 ctattttctt gaaaggaaaa ttctcagcgc ctgcattttt gcccctaaag tgttactcct
    20401 gaagcagcga aacaaatcga accattttcc tctccccaga tagtttaggt tgaagagatc
    20461 ggaacgtttg taatagcaca tgacaaaaat gccctggtca tcgtccacaa tgttattgtt
    20521 tagtgttatt ttttgacttt ccagaactaa tttatagaat tctttccatt tatgctgtga
    20581 accaacaatt gcgcccccaa taatatatac atgattgcca atcatgaaat caaaagcctg
    20641 ttgttgagat gtaacagtaa gccctttttt tatagtgaaa agatgcatct tattttcatc
    20701 aaatgggaaa tcccaaattt tcaatcctcg agttacattt ggcttacggc aatagccaaa
    20761 atctatccac gcaacaagag gcgttttcac taaacccatg ttgatagctt tatttacaaa
    20821 ataggactta aggttacata ccaaaacata ctctggtgac cagtactctg ggttttttaa
    20881 ttgtcgaggc tcaagtctat ttgtaaacga ctcatctttt tgaatttttt cgatacgact
    20941 tctgatatat ctaaatttct tttttatatc gataacgata accgttgttg gttttccgtt
    21001 gcgaatcgct tcaatccttg gtttcaaatc gggagaagtg aaaataatca tctcattttc
    21061 aagtgcggcc agacgctcaa agtaactaaa ataaacatca actgaacgag ctagtttttc
    21121 acggaaccct ttattagtag tccaatcccc tctaccaatg tcaaaatatg cagtgataat
    21181 tgtggtggat gatttcatta ctaataagac cattaatata aaaaaagaat tatatcataa
    21241 atattaacag aatgtatact gtatattatc cattcaaatg attccgttat ttatcataca
    21301 tcggaatatt gatactaaag cactattcac atgcaaaacc aacatccgcc atgaaggact
    21361 agctaaaacc caaactagtt tgttgcaatt agcatccttg cacctctatg taaagggctg
    21421 aagggattcg gatgtgatgg tatgattaca gacattcgtg tctgagattg tctctgactc
    21481 cataattcga aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc
    21541 aacatcgtta aggccctgaa tgataaaggc atcaccgata ttctggtggt ggacaacctg
    21601 aaagacggca ccaagtttgt gaacctggtg gatctggata tcgcggacta tatggataag
    21661 gaagacttcc tgatccagat tatggctggc gaagagttcg gtgatgtcga agcgattttc
    21721 cacgaaggcg catgctcttc caccaccgag tgggatggca agtatatgat ggataacaac
    21781 tatcaatact ccaaagagct gctgcactac tgtctggagc gcgaaatccc gttcctgtat
    21841 gcctcttccg cagccaccta cggcggacgc acctccgact ttattgaatc ccgcgagtac
    21901 gaaaaaccgt tgaatgtcta cggttactca aaattcctgt ttgatgaata tgttcgtcaa
    21961 atcctgccag aagcgaactc gcagattgtt ggcttccgct atttcaacgt ttatggaccg
    22021 cgtgaaggcc ataaaggcag catggcgagc gtcgctttcc atctcaacac ccagcttaat
    22081 aacggtgaat caccgaagct gttcgaaggt agcgagaact tcaaacgtga cttcgtctat
    22141 gtgggcgacg tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggtatcttc
    22201 aacctcggca ccggtcgtgc agaatccttc caggcagtag cagatgctac gctggcttat
    22261 cacaagaaag gccagatcga atacattccg ttcccggata agctgaaagg ccgctaccag
    22321 gcgttcactc aggcagatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa
    22381 accgttgctg aaggtgtaac ggaatacatg gcctggctga atcgcgacgc ataagagctc
    22441 tgcatgaaaa tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt
    22501 ctctatcgca cgctccaggc gcgctatccc caggcgataa tcgatgtgat ggcaccggca
    22561 tggtgccgtc cattattatc gcggatgccg gaagttaacg aagctatccc tatgcctctc
    22621 ggtcacggag cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag
    22681 cgctacgacc gcgcctacgt cttaccaaac tccttcaaat ctgcattagt gcctttcttc
    22741 gcgggtattc ctcatcgcac cggctggcgc ggcgagatgc gctacggttt actcaacgat
    22801 gtacgcgtgc tcgataaaga agcctggccg ctaatggtgg aacgctatgt cgcgctggcc
    22861 tatgacaaag gcattatgcg taccgcacaa gatctgccgc agccattgtt atggccgcag
    22921 ttgcaggtga gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa
    22981 cgtccgatga ttggcttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac
    23041 taccactatg cggaactggc aaagcagctg attgatgaag gttatcaggt ggttctgttt
    23101 ggctctgcga aagatcatga agcgggcaat gagattcttg ccgctttgaa taccgagcag
    23161 caggcatggt gtcggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt
    23221 gcagcctgta aagccattgt cactaacgat tctggcctga tgcacgttgc ggcggcgctc
    23281 aatcgtccgc tggttgccct gtatggtcca agtagcccgg acttcacacc gccgctatcc
    23341 cataaagcgc gcgtgatccg tctgattacc ggctatcaca aagtgcgtaa aggtgacgct
    23401 gcggagggtt atcaccagag cttgatcgac attactcccc agcgcgtact ggaagaactc
    23461 aacgcgctat tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg
    23521 tcgatgggcg atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca
    23581 gggattaagt ttgactgggt ggtggaagaa gggttcgcac agattccttc ctggcacgct
    23641 gccgttgagc gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc
    23701 cccataaaag ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca
    23761 gttatcgacg ctcaggggct ggtaaaaagc gcggcactgg tgacacgtct ggcgcatggc
    23821 gtaaagcatg gattggactg gcaaaccgct cgcgaacctt tagccagcct gttttacaat
    23881 tgtaagcatc atattgcaaa acagcagcac gccgtagaac gcacccgcga actgtttgcc
    23941 aaaagtttgg gctatagcaa accgcaaacc cagggcgatt atgctatcgc acagcatttt
    24001 ctgacgaacc tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc gacgacccgt
    24061 gatgataaac actggccgga agaacactgg cgagaattga ttggtttact ggctgattca
    24121 ggaatacgga ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg
    24181 gcggaaggat ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc
    24241 gtgctggccg gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg
    24301 gcactggata gacccaatat cacggtttat ggaccaaccg atccgggatt aattggtggg
    24361 tatgggaaga atcaagtaga gtgtcgttct acaagtatgt ctcttgcaga tttgccagct
    24421 caaaccgttt ttcaaaattt aaatcttgaa ataataacca ataagttgac atcggagata
    24481 agatgacctc aacattattt ttctctctcg agaaaaaaaa ctggatagcg tactggaaca
    24541 gagctctcgt attcttattc attaccacct attttttggg tgggattaca aggtataaac
    24601 atcttattgt tattcttatg acaataacga caatcgtcta tctctgcaaa cggccaaaac
    24661 actatctctc actatttaaa acatttcttt ttggtagtgt tgccatatta actattgctg
    24721 cattgctgtc acttcttcaa tcccctgatg caggtgctag catgaaggaa attttcaaag
    24781 ctattattga gaatacttta ctatgcacaa tagcaatacc ggtcatattg agagacgaga
    24841 aaagagaaga tgtcgaaaaa atcgttttct tctcatttat tagtgcgttg ggcttacgct
    24901 gtttttctga attgattacc tattataagg actatcaaca agggataatg ccattcgcag
    24961 attatagaca ccgtagcatt tctgactcga tggtcttttt attccctgca ttgttaaatc
    25021 tctggcttat caaatcagca aaataccgca tttcttttgt ggttctaagc gttattttta
    25081 tttttctgat attaggaact ttatccagag gggcctggct ttccgtgtta gtcattggat
    25141 taatatggat tctgatgttt aaacaatgga agttactatt agtaggagta atggtttcca
    25201 tcattgcatt gtcggttatt ttcacacata aggagatgac cgcaaagcta acgtataaac
    25261 ttcaacaaac taatagttct tatcgctatg caaatggtac tcaaggcagc gcactcgatc
    25321 taatattaga aaatcctgtt attggttatg gttacggtaa cgttgcatat aaagatgtct
    25381 ataataaacg tgtcattgat tatccagaat ggacctttag acaatcaata gggccacata
    25441 attttgcgct attcatctgg tttggcactg gtttattagg gctggtaagt cttatgatgc
    25501 tatactgtgc aatattgaaa gagtgtataa aaaatggcgt caagaataaa tatcgctcac
    25561 catataatgc atattatata atcttactat cttttatagg ttattttgtt atccgtggaa
    25621 acgtagaaca aattgaacca aatttattag gcgtttacgc cggcttatta ttagcgatga
    25681 aaaacaagta aaaaataaaa aaggctgcat aatgcggcct ttttatttat tcaaaccaat
    25741 tatgaataac ctcttcgaat ctttgagtta cgccatccca actgtagtga tcaaacacaa
    25801 agtcctgacc ttgctttgca actgctgtta attcaggatt cgctaacgtt tttaaaatat
    25861 cgctgcttat ggagtctgca gtcataggtt cttttaaatg gaatcccgta gtattttctt
    25921 tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac aggcttacca gcacccatag
    25981 cttcaattgc caccatgcaa aatggttcct ggaactggga aggaatgacg actaaatctg
    26041 ccagtgggta ataacaatgc atcttttcag gcggtatgct tcctaacata atgcaacgat
    26101 ccttcaaacg ttttgcaatt tccctgacat tgcgttgata tgcacctttg tcacttttac
    26161 tcatttctgt ataatcacca atcacaacta attttaaatt tttgtgagca gcggccaatt
    26221 tttcgaatga ttgcatcaac agcaatatgc ctttgtcagg gacgatacgc ccagcataaa
    26281 aaataatttt ctcttcaggg gagatattaa gttcgcttct agtgatcggc tgaaaattag
    26341 attgataggt ttctaaatca attccatttg gtacaatctc aatatctgca tttgctaaat
    26401 aagattggta atactttttc aagtataaac tagggactat cattttaaca ttttgctcaa
    26461 gcaattttgg ttcaaatgca ttatgcatat gtataaccac tcttgcctga ggagctcgct
    26521 tacgaatttg agtgtatagc ttcatgctat tatgaactac aataacactt tctttcgtta
    26581 taggaaaatc atgtgcaata ttcaatattc tctgtgaata tggaagagga tcaagtcttg
    26641 tccatttttg aaataaccgc ttatatatgc gactgaatcc aaccctatga atactacaat
    26701 tatcattaac aaaggtgtaa tttgagtatc ctggattttt gatacaaaca atacgatttg
    26761 gaaaattggt acgctgagca acctgataca tccacgtttc aacagcagcc gctccgcgag
    26821 gaggaattga aaaaatagga gtaaccgtaa atattatttt atcaaccatc tcgtatatta
    26881 acctttcata acattatatt taattgctgt aaaaacacca tcgagaaatt ttttctggta
    26941 aagcaagtgt ttatattttt ctttgtgctc atgtttttta acagcttttt tataaggtat
    27001 atttctccat ggtgatatat tataaatatt acgaaaataa tctgcagagg cgtagttcgc
    27061 ccaatcatgc catggcttag ttataccagt ataatgtatg aagacagtgt catcattaat
    27121 aaaccgggtg taatattcac tatttttttc ttcaaattct gactttatag tataaataca
    27181 attatatttt ctcggtaata ttttagcatg atgtagaaac attaagttta aaatatcttg
    27241 atccgggtac tttaattttt ttataattga ctcatctgat aatagatcaa agaatttttc
    27301 cgttagtctt tgttttaacc attcccgtaa attaatatac attacaccag agttaaaata
    27361 gctaccgtta aaatcctcat tacacaaacg ctcagcgctt ttactttgca tagcatctac
    27421 atcaattacc actgccccat attcatcttt aaactctaat gctattaact cgttcaatga
    27481 acccttacag acgatatcag catcaagata taataatgaa tccaatcgcg cagaaaaata
    27541 atcaaatgaa agcaaacgat aataaataga tactggccag atatttgatt gaggtagcgc
    27601 cttcaatggc tcagaatcaa tatgatataa tttaattact gtttgatact tcgttgctaa
    27661 ttgtgataat aattcaatat actctttgtc taagtaatca gcgattatgt gaaaggtaaa
    27721 tttgttttgt ttattctttt caagaattga agcaattgaa atcgcagccc caacctggta
    27781 gttctcatca ataccccatg agacattaaa cgtttcccgt tcatcaagtt taacggggcg
    27841 cttgtccagt tcaattatat ctttaaattg agtaagatgt ttaaaatcca atttaattac
    27901 ccttcatcat aatcattatt tgctcttcaa tcctagtttg acttttacat ccctgattac
    27961 ttttttaatt ttctttttga atataagata tgtatagcca aagtctttca attcattttt
    28021 tatattatag tgccgttcaa gatcaatgcg atcttttgct tttaaaacag cggttgtttt
    28081 tttacctgaa aggtcaatta gtcttaaacc tttttcggat actataaaat ttcctttatg
    28141 aggatcgcca gacaccattc catgctgatg caactctttt atcgattctg ataattgatc
    28201 cttcagatct tcagaaattt ccaaatactc atttaacccg acaccttcaa tatactcaat
    28261 gagcataata taataatgag cgaaatttaa tgttttacgt tcagctagca aaaaatagtc
    28321 attaattgat tggatgcctt caccacgtac ccgatcggtt tgataaatta aattttcata
    28381 ataatctttt tttatgcaag attttaagaa acgttcggtc attttatgct ttggcgcata
    28441 aactttaagt acaagaggtc cacgagcagt gtcaataaga atgactttag tatcatcaat
    28501 agaacgaaaa acttttaatg ttttaaccct gcaagttaaa aagtcattta gaacttgctc
    28561 ataaaaggga tcattatctt ttatgaaaac tttcaaccca tttatcgttt tattatatat
    28621 cataatgaat tattttaacc ttaaatcttt agaagcattt ttctttataa tactttaaat
    28681 aattaaaaat gcctgccata taatgctttt gtttaaattt atgttttgca caataccgat
    28741 actgattgct atttacaggt ttaagtaaat cttctttact ccacggagaa gctgctttgg
    28801 cgatcaaaaa actacgtgaa accggataat cagcccactc atgccagggt tttgtaggtc
    28861 ccacatagtg tataaacacg gtgtcatcat tgactggatt atcaactttg tcttttaatt
    28921 catagttaat actatatcgg gtattatatt tctccgaaat aaacttcact ttaccattca
    28981 ataatacatt cagtacatct tgatcaaggt gggtgatttt acttacccaa tctgggtcac
    29041 gcaacatttc aatagctttt gacgaaatgt tatttagatt ccactcatca atatttatca
    29101 gtaaaaaacc agcattgaaa tatccagaag ctaactgtgg tgtagttaat accgaggctc
    29161 gattttgcca ccattcaata tctctttcag ctacaactgc ggcaatttca ttagtagaaa
    29221 attgataatc taagagttct ttaatactac ccttgcaagc aatatctgca tcaagatata
    29281 gtattttttc atgtttatga tagaaataat ctgcaattat aaatcgaaaa tatgttgcgt
    29341 aagtccagtt tttcgtgctt ggtaatgact tcagcttatc acaattgata acataaatat
    29401 taattcttga attatattgt tttgctaaat cagaaaaata taatttgtct ttatcactga
    29461 tataatctgt gaaaacatga aattcacaag agatttctct attgtttaat agaatagatg
    29521 cgatggctac accacatcca aaaagaaaat ttctgtcaat accaaatgct atatcaagag
    29581 tatttttaga tcttatattt tgataatgaa aattatactc aaaaaggatg atgtcactat
    29641 cattgagttg agacatattt attctgtatc caaaattttg gcaaaaataa gttacgggtt
    29701 acaacgattt tcgaatcgtt ctttccctga tttttgtggc ttttgcttct gcttgcgaca
    29761 gcagtccttg ttcctgcttg agaatgtctt taagcggggc ggcaaaatac accttcataa
    29821 agcgccagat atcccgctga gtcaggccaa tattcattga agaaaaataa agccctataa
    29881 gatctttatc gcgccaacga cctggaacgc gcgtgcgaag ctgcgcccgg tgtaggtcaa
    29941 ttaccgaaat ttttaactcc tcttccttac cggaaaaagg caagtgcagc aggaaatgac
    30001 agatataaca gtcacgatgg ttaatgcccg cagcatgcat atcgcgcacc atcgtcgcga
    30061 cacgcttaat aagcatacgc tttacgcgaa catctggtgg gttggtcgcc cagtcagcac
    30121 agtaatcttc cagactgatg gttggtgtca gatcttcggt aataataaac gaggttctgg
    30181 tcagcggatt catgcctttt tcgccgaatg ccaccccata catagtatca acgccgacat
    30241 cccgcagtcg atgaatcgca ttccattcgc ggtctgcgcc taatactggc atccgcaatg
    30301 agagtaaatt tttgattatc tctttcaggg tcgtgccgcg atgccatttg agaaaatagc
    30361 tttttcccgc catttcaaag cgcagagtac ggcgagtttc cagttcacga aatacctcac
    30421 cctgcaaggt tttaacttcc tcaaaaggat ctttgccgcg ccataacgtg gcaaacggct
    30481 ctttaagttc aaccatccag accacccgtt atgatatccg ccgctttctc tggcagactg
    30541 tataaatctt gtgtatcagc ataatgtcgc gcattttccg cccaagcctg gcgcaatgaa
    30601 gattgcgtta acgctttgcg taaaatctca ttcaatgttt cctggcggaa tggctcagca
    30661 atagcctcgc cgcaattagc gtcgacaata taatgcgcat agccacaaac ggcagttgtt
    30721 agtaccggta atcctgcagt aatcgcttcc agcagcacaa ttcccgccgc ttcctggtag
    30781 gcaggatgca gtaataaatc cgccgccgcc attaattccg agacatcgtt gcgccccgag
    30841 aagaagtgaa cattactgcg cacgccgcgt ttttctgcca gtgcctcaaa ttttcgcggt
    30901 ttatcctgcc caacaacata tagcaatgtg ttgtggcgca gcgaatccgg taacgaagca
    30961 agtgcttcaa tggaacgatc gacaccttta cgcgtgaagt ctgaaccgac ctgcaacaat
    31021 aaatattgtt gttcggttat tccattcttc ttacggaaga tttcacggct attggctggc
    31081 tgctggctat atttacgatc aggataaatc cccggtggca gaatatgaaa acgctccgct
    31141 tcagtctgat aatgtttctg gaaatcggcg atttgcttat ctgtcagcat cagcagctgt
    31201 gtcggcttgc cctgttcgaa ggttgcccgc tcaaaggcgg cataatggcg ataacgtgac
    31261 gtcaggcgat agaaaaagcc tttttcctgc gctactttct cggcataaca aacatcagcg
    31321 gcataataaa cgtccagccc cggcattttg ttgaatccaa cgactttatc gacgggatgt
    31381 tcgcgtaaat gtttttgcac ccaggcaaaa tactccgcat tgcgcccgtg attggtatgc
    31441 gatttaaccg gcactttgat cagttcaaat acatcagggc attcgccttc ccacgactgg
    31501 gtataaaccc gaacatgatg acctcgggcg gcgactgtct gagcaatacg cataaaatcg
    31561 cgctgcaaac cgccaaaggg aaaatattta tataaacaaa aagcaacgat cataacgacg
    31621 tacctgtcgt ggaggagggc agtaatttat cgacagcagc aatgacatct gccgccggaa
    31681 taaccgaaag atacatctca tttcggtcac gctgatcgcg cgttggcatt tcccggtaat
    31741 ctcccgccca gaattgaatc atgttatttg accagggacg ccagaaaata tgatctgttg
    31801 cgccaaacag cgatatcagc ggcgtattaa ctgcagcggc aatatgcgcc ggtgcggaat
    31861 caacgccaat aaacagctgc gcatgatcga ttaacgcacc aagttccggg aaggtcacct
    31921 ttccagccag cgccgttact ggtggcgtct ggcatccctg cgcaatttca ttgacgcagg
    31981 ccagatcgtc tttatctggg ccggacgtca gaacgacttc ataaccacga gcatgtaagg
    32041 catcaatcac agcggaaaac ttggcgttgt cccagcattt gaagatttgc cgcgccgtag
    32101 gttggataac cacataattt tgtccaacac cagcgtgatc aagttcgcga cgcatacgtt
    32161 tccagcttgc aggcgggtaa ctcatggttg tctgcttcac caacgaatca agtcccaatg
    32221 gtgtcagcac ggataagtta ctttccacca catttccacc ctgcaacggc accaaatggg
    32281 tgaaactttt acgccaaaaa gcagactgcc gatgatgata atcctgggaa attttcacac
    32341 gagcatttaa taagcgaacc agtatagcaa ccatccattg atcggtaaga ttgacgataa
    32401 ggtcatactt attggcacgt aataccttga tgagatgaaa aaagttggca attttttctg
    32461 aggcttttgc ttttttattt tttatgccgt agagcgcgtt aatctctgga ttttcagaca
    32521 ggatcgggat ggtgtcctga taaagcagca catcgatttt tgcgtcaggg taattttttt
    32581 tcagagaact aatgacggga gtagttaata acatatcccc atgaaaacgc atcttaatga
    32641 gcaaaattct tcgaaatggc ttatccacaa gtaactcttt ttgttaagat tatccgatta
    32701 aggtgaagca gaaaaagcac gctaccgccc caggctcaac agctacctga atactgatac
    32761 cgcactgata ttgacgcgct agtgtatcac ttctttcgcg gcaatccgac ccgaatgatt
    32821 tttaagcaca ttgttgattt tcaataaaga aatcaatcaa ataaaatcat tagcaaccat
    32881 attctttcac tgttgctctg cgattccttc ctgcacgtac agcagaaatc aatgaatcgc
    32941 ataaaaatac aaaaaaagta tagatgttca gcaagaattg gcgggtaaat tgctatttaa
//
LOCUS       DADLTF010000030        32717 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.030, whole genome shotgun sequence.
ACCESSION   DADLTF010000030 DADLTF010000000
VERSION     DADLTF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.030"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..869)
                     /locus_tag="JN749_001336"
     CDS             complement(<1..869)
                     /locus_tag="JN749_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HAY7367806.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLP"
     gene            complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="JN749_001337"
     CDS             complement(1047..1958)
                     /gene="gsiD"
                     /locus_tag="JN749_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HAY7367807.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="JN749_001338"
     CDS             complement(1961..2881)
                     /gene="gsiC"
                     /locus_tag="JN749_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HAY7367808.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="JN749_001339"
     CDS             complement(2899..4437)
                     /gene="gsiB"
                     /locus_tag="JN749_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HAY7367809.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="JN749_001340"
     CDS             complement(4457..6328)
                     /gene="gsiA"
                     /locus_tag="JN749_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HAY7367810.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="JN749_001341"
     CDS             complement(6315..7280)
                     /gene="iaaA"
                     /locus_tag="JN749_001341"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7367811.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7484..8719
                     /gene="moeA"
                     /locus_tag="JN749_001342"
     CDS             7484..8719
                     /gene="moeA"
                     /locus_tag="JN749_001342"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HAY7367812.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPISATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8719..9468
                     /gene="moeB"
                     /locus_tag="JN749_001343"
     CDS             8719..9468
                     /gene="moeB"
                     /locus_tag="JN749_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HAY7367813.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="JN749_001344"
     CDS             complement(9647..10309)
                     /gene="fsa"
                     /locus_tag="JN749_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAY7367814.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10440..11339
                     /locus_tag="JN749_001345"
     CDS             10440..11339
                     /locus_tag="JN749_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HAY7367815.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11345..13777
                     /locus_tag="JN749_001346"
     CDS             11345..13777
                     /locus_tag="JN749_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HAY7367816.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13923..14738
                     /gene="ybiV"
                     /locus_tag="JN749_001347"
     CDS             13923..14738
                     /gene="ybiV"
                     /locus_tag="JN749_001347"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HAY7367817.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14890..16155
                     /locus_tag="JN749_001348"
     CDS             14890..16155
                     /locus_tag="JN749_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HAY7367818.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16396..17988)
                     /locus_tag="JN749_001349"
     CDS             complement(16396..17988)
                     /locus_tag="JN749_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HAY7367819.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPECVIDFSGNYEDYLRSKGIE"
     gene            18207..19127
                     /gene="ldtB"
                     /locus_tag="JN749_001350"
     CDS             18207..19127
                     /gene="ldtB"
                     /locus_tag="JN749_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7367820.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(19185..20303)
                     /locus_tag="JN749_001351"
     CDS             complement(19185..20303)
                     /locus_tag="JN749_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HAY7367821.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(20300..20767)
                     /locus_tag="JN749_001352"
     CDS             complement(20300..20767)
                     /locus_tag="JN749_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HAY7367822.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20953..21081
                     /gene="mntS"
                     /locus_tag="JN749_001353"
     CDS             20953..21081
                     /gene="mntS"
                     /locus_tag="JN749_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HAY7367823.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            21353..22936
                     /locus_tag="JN749_001354"
     CDS             21353..22936
                     /locus_tag="JN749_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HAY7367824.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="JN749_001355"
     CDS             complement(22984..23499)
                     /gene="ompX"
                     /locus_tag="JN749_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HAY7367825.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23552..23617)
                     /locus_tag="JN749_001356"
     CDS             complement(23552..23617)
                     /locus_tag="JN749_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367826.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23852..24739
                     /gene="rhtA"
                     /locus_tag="JN749_001357"
     CDS             23852..24739
                     /gene="rhtA"
                     /locus_tag="JN749_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HAY7367827.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            25038..25541
                     /gene="dps"
                     /locus_tag="JN749_001358"
                     /gene_synonym="pexB"
     CDS             25038..25541
                     /gene="dps"
                     /locus_tag="JN749_001358"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HAY7367828.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25945..26691
                     /gene="glnH"
                     /locus_tag="JN749_001359"
     CDS             25945..26691
                     /gene="glnH"
                     /locus_tag="JN749_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HAY7367829.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            26830..27489
                     /gene="glnP"
                     /locus_tag="JN749_001360"
     CDS             26830..27489
                     /gene="glnP"
                     /locus_tag="JN749_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HAY7367830.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27486..28208
                     /gene="glnQ"
                     /locus_tag="JN749_001361"
     CDS             27486..28208
                     /gene="glnQ"
                     /locus_tag="JN749_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HAY7367831.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            28630..30693
                     /gene="ybiO"
                     /locus_tag="JN749_001362"
     CDS             28630..30693
                     /gene="ybiO"
                     /locus_tag="JN749_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HAY7367832.1"
                     /translation="MLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQK
                     VTEVSRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIR
                     LCALPLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNA
                     GSRTIAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLS
                     SLIGYGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAE
                     HSLAFFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAA
                     FVSGMFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAW
                     GLFDFWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSA
                     RTRTLLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDI
                     ITGVFIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANF
                     VRGIGSVVANYDVDRHEDADKANQALKDAVTELMENEEIRGLIIGEPNFAGIVGLSNT
                     AFTLRVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPP
                     GEPTL"
     gene            complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="JN749_001363"
     CDS             complement(30690..31616)
                     /gene="rlmF"
                     /locus_tag="JN749_001363"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HAY7367833.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            31892..32152
                     /locus_tag="JN749_001364"
     CDS             31892..32152
                     /locus_tag="JN749_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7367834.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            32417..>32717
                     /locus_tag="JN749_001365"
     CDS             32417..>32717
                     /locus_tag="JN749_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HAY7367835.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT         8022 a         8736 c         8097 g         7862 t
ORIGIN      
        1 ggtagataaa aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag
       61 atttcgaaca atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca
      121 gaatcaataa tattataatt accttctcta ttataaataa tcagtttttc acgagcgagt
      181 tgattcttgg caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg
      241 atttgatcaa gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc
      301 gctaacaaat cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc
      361 ggacgatcaa agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc
      421 gaccacggtc gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca
      481 agcggtccat ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa
      541 aagagataat atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt
      601 tccattttca gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag
      661 tcctttaatg tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga
      721 ataagttcaa agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta
      781 atcatgaaaa aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt
      841 atctttatct tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa
      901 cgctgtgccg ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct
      961 acaaaatcag cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta
     1021 ccctttcaag caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt
     1081 aaaccatcgc ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa
     1141 acagcgacat gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac
     1201 tctggtgtcg gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc
     1261 gaggtgccaa tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata
     1321 tgacgcaata aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt
     1381 tgctgtttca acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa
     1441 atggcgactg cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc
     1501 gccagtaaaa tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc
     1561 caccagcctt cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata
     1621 aacacggcaa acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa
     1681 atgtcgcgcc ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga
     1741 ttgtcataat caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc
     1801 accacaatca ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat
     1861 cgtcgccaga attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg
     1921 tttaacaccg cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg
     1981 ggttaatggc ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga
     2041 aaagcagaat ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta
     2101 agcgtccaag tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa
     2161 agccaaactg taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt
     2221 tgaggacaac ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc
     2281 ttaaaacgtc gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc
     2341 cgagcgtcag ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc
     2401 agcccagttc aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg
     2461 agatccccga caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg
     2521 cggcgataat tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca
     2581 gccacagcgt tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca
     2641 tcgacaggcc aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca
     2701 gcggctgatc caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg
     2761 gcccggcaat caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca
     2821 ccgagacgat aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca
     2881 ttccactccc tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg
     2941 catgatccaa aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag
     3001 cgggatccac ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt
     3061 ttccgccgga tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct
     3121 gtaaaacgcg gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag
     3181 tgcccagtcg gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct
     3241 ctctttttgc cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac
     3301 ctgggcttta atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc
     3361 ggtgctgtgg ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc
     3421 tttcagtaat tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta
     3481 ggcgatactt ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt
     3541 caccagcgcc ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa
     3601 cggcttttgc gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa
     3661 ctcgatattt ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa
     3721 ctgcgcttca ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca
     3781 ggttatgctg tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt
     3841 cacaaaatcg gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa
     3901 accaatctcc ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg
     3961 ggcaagaata ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg
     4021 atcgatcgcc tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc
     4081 cggatcgctg gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc
     4141 ctggaattta attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt
     4201 ataactctcc gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg
     4261 gtaaaacgat ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt
     4321 ggtgaaattc gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat
     4381 caacgctgtc gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt
     4441 attctccgcc tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt
     4501 aatgccccgg cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa
     4561 tattgctggg aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag
     4621 cgaccggaac tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg
     4681 cgcaccgtga accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa
     4741 tccgctctac cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct
     4801 ggagatcgag caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg
     4861 cttcgtcggc aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct
     4921 ggcgctgacc gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca
     4981 cgcgctccag caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta
     5041 cacgcagcgg ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag
     5101 ggtcctgaaa aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg
     5161 tatcgattcg ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca
     5221 acgcccgccc ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc
     5281 caggccagag atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca
     5341 acaaaccgct gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc
     5401 catccaccac cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata
     5461 tcaatgggaa acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag
     5521 ctaacagcgc acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg
     5581 tttccaccgc ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc
     5641 ccatatcgtg agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta
     5701 attgcaggat ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca
     5761 gcaccgccgg gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag
     5821 agagttgatg cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca
     5881 gcatccgctt cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg
     5941 attcggcaat ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa
     6001 atatcatcgc catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa
     6061 gttcaatcac atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc
     6121 ccgcctgttc caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt
     6181 cgcctacaat tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag
     6241 ctattttctg ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac
     6301 cggcatcaag ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg
     6361 gttggcgtat cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc
     6421 gcgacattcc cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt
     6481 ttttccatta ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac
     6541 gcggcgatgt catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa
     6601 accgccacac tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact
     6661 cgtccgggta atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct
     6721 aaatccaacg ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg
     6781 ctatggtcga ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa
     6841 ggcgtggaga aaatctccgg tgagacgcgc tccatgccat gagcaaacgc aaaattttct
     6901 gccccttcgc caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga
     6961 accggattac gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc
     7021 atcacacagg cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg
     7081 ttaaacagtg gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt
     7141 tcgccggctt ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag
     7201 cgtaattcct gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt
     7261 gcaatgactg ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata
     7321 aatatcagaa tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc
     7381 atgtaaaggc aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg
     7441 acataatagg caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg
     7501 attgatgtcg ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac
     7561 ccaggaaacg ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc
     7621 gccactggat gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc
     7681 cgatattgcc tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata
     7741 ccatggtgaa tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga
     7801 aggctgcgaa gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt
     7861 tactgctgaa gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg
     7921 tgcggttgtt ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc
     7981 actggggatt gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg
     8041 tgatgaactc cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg
     8101 tctcgccgta cacctgatgt tagaacagct gggttgcgag gtaatcaact tagggattat
     8161 ccgcgacgat ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt
     8221 agtgatcagt tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga
     8281 agagctgggg gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt
     8341 cggtaaactc agcaatagct ggttctgcgg cctgccgggc aacccgattt cagcgacgct
     8401 gaccttctat caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg
     8461 cctgcccgcg cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct
     8521 tgatttccag cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac
     8581 cggacatcag ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct
     8641 ggaacgcgat cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc
     8701 gttgttcgga ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg
     8761 caaatcattc tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg
     8821 ctggtagtcg gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt
     8881 gtcggcaacc tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag
     8941 acactgcaca gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg
     9001 acgcggatca acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa
     9061 cttgcagcat tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta
     9121 cgtaatcaac tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca
     9181 attcgtatgg aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc
     9241 tgccttagcc gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca
     9301 ccgttgatcg gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt
     9361 tatggcaaac cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc
     9421 gaaatgaaac tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca
     9481 ggcctgataa gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg
     9541 tgaacgcctt atctggccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg
     9601 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     9661 tgccaaacgc tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat
     9721 agctaatcat ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta
     9781 agcagtccag cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg
     9841 catgcatttt caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag
     9901 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc
     9961 cttgtgccgc gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct
    10021 taatcgctgc cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag
    10081 aacgcagctt aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
    10141 acagacgccc ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt
    10201 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
    10261 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
    10321 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
    10381 catcaatcta actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta
    10441 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
    10501 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta
    10561 cgcaggatct gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg
    10621 ccgcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
    10681 ccccggagca tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg
    10741 gtgaagtgaa aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg
    10801 atcgcagcgg cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg
    10861 cgacggcgct gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc
    10921 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10981 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg
    11041 tgctggataa cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc
    11101 tgattcaggg ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg
    11161 acgagctgca cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat
    11221 atcacttact taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc
    11281 tcgattttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    11341 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    11401 gctggtgcat attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat
    11461 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca
    11521 cctggcgaat cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag
    11581 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat
    11641 tgatgatctg gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt
    11701 tctgcatgaa gtgtgcccgt ggtggcgcgg tcagacggta caggatcgct gctatggcat
    11761 gtttaccgat gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat
    11821 gacctccggc gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga
    11881 tggtctgcgt gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga
    11941 tttgcacggc gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca
    12001 cattgaacgt ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg
    12061 tcgcgatgaa ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca
    12121 gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc
    12181 taacggtcac tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg
    12241 cgacgttgaa ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg
    12301 ctggttgaaa ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc
    12361 gggaagtccg ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc
    12421 aatggacgcg gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc
    12481 cactcagcct aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc
    12541 ctgcgtacag gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt
    12601 gatcccggaa tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat
    12661 tggttgtata gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    12721 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg
    12781 caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga
    12841 agtgatggcc gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga
    12901 atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt
    12961 ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt
    13021 ttctggcctg caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact
    13081 ggtgtttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    13141 cgatggcctg actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg
    13201 caacgacgat gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    13261 actgaaacag taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc
    13321 gggtacgtcg tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga
    13381 cgggcgtaaa gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca
    13441 tctcggccct actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg
    13501 cggcgtgttg ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca
    13561 gaaactgatg atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta
    13621 caacatcgtt tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga
    13681 tctggtagtg cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca
    13741 ggacgatatc atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt
    13801 cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt
    13861 gtgctctgcg tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac
    13921 ctatgagcgt aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa
    13981 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt
    14041 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    14101 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt
    14161 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    14221 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg
    14281 aagcatttgt cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc
    14341 aggagattga cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt
    14401 tagtgatcga caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt
    14461 ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    14521 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    14581 cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta
    14641 aacaaatcgc ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc
    14701 aggctgtgct ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc
    14761 tccggttttc ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa
    14821 cttatttata acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc
    14881 gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct
    14941 atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat
    15001 cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc
    15061 gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag
    15121 cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa
    15181 caagcgctag gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag
    15241 gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt
    15301 taccccatct actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat
    15361 gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac
    15421 ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc
    15481 aaaggtcttg gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat
    15541 cagcacgttt tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg
    15601 gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt
    15661 cggacattcc agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac
    15721 gtcgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg
    15781 ccggaggatg aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg
    15841 catccactgt taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc
    15901 tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc
    15961 aacgtgatgt acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag
    16021 gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa
    16081 acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg
    16141 ggcatggatg tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg
    16201 acgttactca gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt
    16261 atccggccta caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag
    16321 cgtagcgcat caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg
    16381 gtgacggata atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct
    16441 aaagtcgatc acgcattccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa
    16501 ctcacggtcg tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag
    16561 tgactcgatg gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg
    16621 cttctgcatc attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt
    16681 agctggcttt ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc
    16741 ctgctcgtca tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt
    16801 ttcaaactca tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt
    16861 tacggtgccg ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc
    16921 gacgccgttg gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt
    16981 tttaaacagt ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg
    17041 gaacagtttc ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac
    17101 ctcttccagt ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga
    17161 ggcgttggcg ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt
    17221 ggcgttatcg gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta
    17281 gttacccgga taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat
    17341 gttaaggaag tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac
    17401 ctgttccagc cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag
    17461 aatatccggc tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc
    17521 aacttcactc atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc
    17581 accggcgcga gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc
    17641 cactttatag ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg
    17701 cttcacttcc cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc
    17761 aaaggcaaac tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt
    17821 acccagcgtc ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc
    17881 gttcgcgcca atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa
    17941 caacggctta ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg
    18001 aaaagagata tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg
    18061 cttaaggtct acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac
    18121 tgtgagctgg aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc
    18181 ctgccaggca caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg
    18241 ccttcgctgt tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg
    18301 acgggagtcg cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc
    18361 cgctggagta ttttgccgcg gagtaccaga tggggctttc caatatgatg gaagcgaacc
    18421 cgggtgtgga taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga
    18481 tcctgccgga taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt
    18541 actatccgaa agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca
    18601 aagatacgcc tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga
    18661 cgccgaccgc caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg
    18721 ttccggcagg tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg
    18781 ctatccatgg caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc
    18841 gtctacgtaa cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac
    18901 agtttattga tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag
    18961 tccataaccc gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta
    19021 ccctgacgaa gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg
    19081 atgaagcgat caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta
    19141 aaacaaaggc ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc
    19201 ataactaaca aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga
    19261 tggaaacgcc accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc
    19321 gatcctggta ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga
    19381 ggtacataat tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg
    19441 actgccgtta accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc
    19501 aacgctggaa gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc
    19561 aacgtccagt ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca
    19621 atcaccagtc cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc
    19681 cccagacaat tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat
    19741 tgcagcgcct ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg
    19801 ccagccagcg gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag
    19861 ataagaatat tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc
    19921 gcctcaaaaa taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc
    19981 agcggaacaa caataaacag cgcgacatcg ttggtcagaa aggtagaaag cagcgccgcc
    20041 gccagcacca taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc
    20101 cccagcacat caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt
    20161 aaggtgatga tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg
    20221 aaaaagctta atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc
    20281 gtgcgtaaaa aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt
    20341 cgagcgtctc ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg
    20401 gactgacgcc caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct
    20461 gcgccagctt ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca
    20521 gccccatggt tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac
    20581 gagcagccat gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct
    20641 caacgtaatc atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa
    20701 cgtgctcttc cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac
    20761 gactcattgt gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca
    20821 ttgtaaacca gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat
    20881 agcctgtgct atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag
    20941 tcaggaggtc ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt
    21001 aaagtacggt taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa
    21061 gataaacctt cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca
    21121 ggcataacgc gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc
    21181 aaaagatttg ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc
    21241 tgcgtatatt gcggctcgcg gatgcggacc cctttcccct cttcacgcac tcttgcaggt
    21301 attgaccctt gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt
    21361 aaccctcaaa gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta
    21421 ctttttacag tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac
    21481 cgctgcgttt acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt
    21541 tctggtcggt gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg
    21601 cgcgcctaat ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga
    21661 aatcctgacg atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg
    21721 cgtaatagca atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag
    21781 cctgtgtctg gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg
    21841 gggtggcgta tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt
    21901 catcgtcaat aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat
    21961 gaaagatacc tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat
    22021 cggtgaaagc gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc
    22081 gtttgccagc agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac
    22141 gcagaaatcg cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga
    22201 taactttgtc accctggcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg
    22261 ccaaatcggc gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta
    22321 cttcctgaaa gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga
    22381 tatgaccgct caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct
    22441 gatgggctcg catccgcagg cctgcgacag gacacaaggc aaatacgaaa cctttgtgca
    22501 atcgaaagaa acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa
    22561 gctgtacgat cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca
    22621 cggtctggcc tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata
    22681 tcagcaaaat ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt
    22741 gattaaagcc cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat
    22801 taaagcgaag gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat
    22861 atacatcacc aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt
    22921 cgacccgaaa ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag
    22981 tgattagaag cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg
    23041 gctctgctcg taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc
    23101 accgtaggag aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg
    23161 gaatttacca taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta
    23221 agccggacca gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt
    23281 acggcttttc tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc
    23341 atagcggtat ttcaggttga aaccgcccat tttgttcatt tggccctgag cgtcgctctg
    23401 tgcgtaaccg ccagttacgg tagaagtcgc agctacggaa gtgcctgcgg tgaaagccag
    23461 aactgcggcc agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca
    23521 aataagtcct aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat
    23581 gtctaacggg ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta
    23641 aatcactttt tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga
    23701 ttattcctaa tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg
    23761 ggaaaatttt ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt
    23821 tacttaagtc tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt
    23881 ctggttacca atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt
    23941 agctaagtca ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt
    24001 aggaacgctg atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca
    24061 acggttaccg ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct
    24121 ttctattcag acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc
    24181 ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct
    24241 tggtctgtgg ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg
    24301 tgcgctggca ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc
    24361 aggagcggaa catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt
    24421 cgtgccaatt ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt
    24481 gggtctggct gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct
    24541 cacccgtttg ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc
    24601 cgtttccggg atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg
    24661 cgctatcatc gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa
    24721 agaattagac attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt
    24781 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
    24841 cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg
    24901 aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca
    24961 gaatagcgga acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
    25021 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
    25081 tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc
    25141 gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
    25201 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg
    25261 atcatctgga taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca
    25321 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
    25381 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
    25441 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
    25501 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
    25561 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca
    25621 cctcaacgca cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt
    25681 gtatccacat catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta
    25741 aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc
    25801 atcttcaggg tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
    25861 atgagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
    25921 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
    25981 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
    26041 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
    26101 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
    26161 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
    26221 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
    26281 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
    26341 gtggttgctg tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
    26401 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
    26461 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
    26521 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg
    26581 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
    26641 ggaacttaca acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac
    26701 acctgtaaag cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc
    26761 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
    26821 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
    26881 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
    26941 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
    27001 tttattgaag tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc
    27061 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
    27121 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac
    27181 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
    27241 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
    27301 agcattaaag acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg
    27361 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
    27421 tatctgatta ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa
    27481 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgctgcaca
    27541 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
    27601 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
    27661 ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag
    27721 caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca
    27781 tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg
    27841 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
    27901 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
    27961 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
    28021 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
    28081 ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca
    28141 atccgcaggt gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
    28201 tctcttaata agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc
    28261 aacttattaa aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa
    28321 aaaagcccgc acgttgtcag caacctgctt aatatccctt cctccctttc acccgaaagg
    28381 gaggcacacc agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt
    28441 tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct
    28501 gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga
    28561 ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca
    28621 ctggcggatg tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact
    28681 gtcgccgcga cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa
    28741 gagcaaaccg tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc
    28801 gcccgcttcg ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca
    28861 caaaccttca gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt
    28921 tactggctga ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg
    28981 caaaaaaatc gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt
    29041 attatcgacc tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac
    29101 ctgaatgcag gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc
    29161 ctgattgaat ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg
    29221 cgcccgttca cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta
    29281 agcagcctga ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat
    29341 gtacagatag gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg
    29401 tacctgatct tccgaaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat
    29461 tcgctggctt ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca
    29521 agcgcctatt ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa
    29581 tttatgatgg gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc
    29641 ggtatgtttt cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac
    29701 tatccggagc tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt
    29761 attctgaccg tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc
    29821 tggaactggc tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca
    29881 ctcattcttt tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg
    29941 ctggcttcgg atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg
    30001 ctgttccgta acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg
    30061 gaaatcggcg tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc
    30121 tcgtttggtt cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa
    30181 aacggcatga acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg
    30241 atgtcgattc gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg
    30301 tcttcgataa ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac
    30361 gatgttgatc gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg
    30421 gaattgatgg aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg
    30481 attgtcggct taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc
    30541 aaacagtgga cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg
    30601 ggcgttcgcg cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct
    30661 gaaccgctgc cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc
    30721 tgctcgtcgt tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc
    30781 tcttttttta ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag
    30841 ttttcaccac gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc
    30901 tcttcaatca tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc
    30961 tgaccaccaa agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca
    31021 ctacctgccc gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg
    31081 tattgctcgt ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc
    31141 agacgaatgg cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac
    31201 gcctggctgc tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc
    31261 ggataaatac agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt
    31321 ccgcttgctt cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact
    31381 ggtgggcaga gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc
    31441 aacgccttat tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct
    31501 gcgggtgtaa gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga
    31561 tcgtagcggc tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca
    31621 ttctccttta agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg
    31681 cgtttatcat tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat
    31741 cagcattatg catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa
    31801 gcatatctct gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg
    31861 cactacccgt tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc
    31921 caccgtcctg agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga
    31981 cagcggtcag ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga
    32041 tctggaggat aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa
    32101 ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca
    32161 acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa
    32221 atatgaacgc aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc
    32281 gtcaagtgat aatgcttatc aaaattatta tcacttgcac aaacactatc acgggattaa
    32341 cagtggcatc gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga
    32401 aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct
    32461 cacgttcttt gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga
    32521 agggcaagct aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc
    32581 gccacaacaa tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat
    32641 gacggtgatt tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct
    32701 caaaaacgtc cccggcg
//
LOCUS       DADLTF010000031        32328 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.031, whole genome shotgun sequence.
ACCESSION   DADLTF010000031 DADLTF010000000
VERSION     DADLTF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.031"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JN749_001366"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="JN749_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HAY7367836.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNKFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="JN749_001367"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="JN749_001367"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAY7367837.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="JN749_001368"
     CDS             complement(3736..4092)
                     /locus_tag="JN749_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7367838.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="JN749_001369"
     CDS             complement(4096..4512)
                     /locus_tag="JN749_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAY7367839.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /locus_tag="JN749_001370"
     CDS             complement(4623..5336)
                     /locus_tag="JN749_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B acid phosphatase"
                     /protein_id="HAY7367840.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5699..6241
                     /locus_tag="JN749_001371"
     CDS             5699..6241
                     /locus_tag="JN749_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005059113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367841.1"
                     /translation="MVINFITRDGDDDMNISYVNSNKTTSLPVELDALNNKDISYAKD
                     FSYAKDFFLYIETQLKIAKDFCRPGEEVSSSIASKVFHAFIDLVNKIRGKKDFMYICT
                     LCCFAEEVKGDYSHYRTFLFDIGNQYKVKLIQSGKKEFSLTLEFNDTIIESQKVTGNK
                     AKHILEDIEIFYRNKPDTYY"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JN749_001372"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="JN749_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HAY7367842.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JN749_001373"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="JN749_001373"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAY7367843.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /locus_tag="JN749_001374"
     CDS             complement(9042..10457)
                     /locus_tag="JN749_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HAY7367844.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="JN749_001375"
     CDS             10540..11523
                     /locus_tag="JN749_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HAY7367845.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQPAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JN749_001376"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="JN749_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HAY7367846.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JN749_001377"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="JN749_001377"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HAY7367847.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14172)
                     /locus_tag="JN749_001378"
     CDS             complement(13465..14172)
                     /locus_tag="JN749_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HAY7367848.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14196..14450)
                     /locus_tag="JN749_001379"
     CDS             complement(14196..14450)
                     /locus_tag="JN749_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367849.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            14768..15283
                     /gene="zur"
                     /locus_tag="JN749_001380"
     CDS             14768..15283
                     /gene="zur"
                     /locus_tag="JN749_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HAY7367850.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15325..15534)
                     /locus_tag="JN749_001381"
     CDS             complement(15325..15534)
                     /locus_tag="JN749_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAY7367851.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15650..17029)
                     /gene="dinF"
                     /locus_tag="JN749_001382"
     CDS             complement(15650..17029)
                     /gene="dinF"
                     /locus_tag="JN749_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709876.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HAY7367852.1"
                     /translation="MPPGVAVCFSSLFIRLVCMAFLTSSDKALWHLALPMIFSNITVP
                     LLGLVDTAVIGHLDSPVYLGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQA
                     LARALVQPLLLALGAGALIALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPAS
                     LANLVLLGWLLGVQYARAPVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYA
                     TLLIGLLMVRKILKLRGISGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVL
                     GARLGSDIIAVNAVLMTLLTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACR
                     QSGIVALLFSVVYLLAGEHIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMF
                     IGATRAAEMRNSMAVAAAGFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHW
                     RNGTWFAAT"
     gene            complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="JN749_001383"
     CDS             complement(17048..17656)
                     /gene="lexA"
                     /locus_tag="JN749_001383"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HAY7367853.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17766..18134)
                     /locus_tag="JN749_001384"
     CDS             complement(17766..18134)
                     /locus_tag="JN749_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAY7367854.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18305..20728
                     /gene="plsB"
                     /locus_tag="JN749_001385"
     CDS             18305..20728
                     /gene="plsB"
                     /locus_tag="JN749_001385"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HAY7367855.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="JN749_001386"
     CDS             complement(20883..21755)
                     /gene="ubiA"
                     /locus_tag="JN749_001386"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HAY7367856.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="JN749_001387"
     CDS             complement(21768..22265)
                     /gene="ubiC"
                     /locus_tag="JN749_001387"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HAY7367857.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22488..24068)
                     /locus_tag="JN749_001388"
                     /pseudo
     CDS             complement(22488..24068)
                     /locus_tag="JN749_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24297..25217)
                     /gene="malM"
                     /locus_tag="JN749_001389"
     CDS             complement(24297..25217)
                     /gene="malM"
                     /locus_tag="JN749_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HAY7367858.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLSVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="JN749_001390"
     CDS             complement(25369..26709)
                     /gene="lamB"
                     /locus_tag="JN749_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HAY7367859.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26781..27896)
                     /gene="malK"
                     /locus_tag="JN749_001391"
     CDS             complement(26781..27896)
                     /gene="malK"
                     /locus_tag="JN749_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HAY7367860.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28261..29451
                     /gene="malE"
                     /locus_tag="JN749_001392"
     CDS             28261..29451
                     /gene="malE"
                     /locus_tag="JN749_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HAY7367861.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29605..31149
                     /gene="malF"
                     /locus_tag="JN749_001393"
     CDS             29605..31149
                     /gene="malF"
                     /locus_tag="JN749_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HAY7367862.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITTILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31164..32054
                     /gene="malG"
                     /locus_tag="JN749_001394"
     CDS             31164..32054
                     /gene="malG"
                     /locus_tag="JN749_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HAY7367863.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8228 a         8293 c         7804 g         8003 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaacttatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaggttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa cctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aatagtgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt tttacgtgca
    14221 tgccataaat tatgcgtgat tcgttgaata aacaaatcta tttttgcaat aattatatcc
    14281 cgatgacgca gcgctgcata atcaatagat ttaccatata tattatttaa tttacctttg
    14341 attgaacctc tattatccaa tttatctttg gttgcatctt tattttcgaa ttgatttgaa
    14401 ttgagtgttt tattaaagta tccagttgca ccgggtatta tcttcaacat gattcatcta
    14461 ccttttttgt cgtaccgtgt cctccttata attcttttgc catctggtca ctatcaaaaa
    14521 tcgctcataa tttctgaact acaggtatgc gtttaaaccc agttaaataa atagcgagtt
    14581 aacttgaagc aatttattta aaaatgaatt attaattatt atcgtgttga taatggtacg
    14641 acaaggcaac acaacacctt acctacaaaa cagaaaatca acattacggc aacaataagg
    14701 gttctcggtg tttttcctgg aacatggtaa agtaaggaca ttcttaaccc ccactttgag
    14761 gtgcccgatg gaaaagacca caacgcagga gttattagcg caggctgaaa aaatctgcgc
    14821 gcagcgtaat gtgcgcctga ccccacagcg cctggaagtg ttgcgcctga tgagtctgca
    14881 ggatggcgct atcagcgctt atgatctgct tgatttactg cgcgaagctg aaccgcaagc
    14941 caagccgcca acggtttatc gcgcgctgga ttttctgctt gagcaaggtt ttgtgcataa
    15001 ggtggaatcc accaacagtt atgtgctctg tcatctgttc gaccagccca cgcatacgtc
    15061 agccatgttt atttgcgatc gctgcggcgc ggtgaaagaa gagtgtgcag aaggcgtgga
    15121 agacattatg catacgctgg cggcaaaaat ggggtttgcc ctgcggcata atgtgattga
    15181 agcacatggg ctctgtgcgg catgcgtgga agtggaagcg tgtcgtcatc ctgaacagtg
    15241 ccagcatgat cactctgtgc aggtgaaaaa gaaaccgcgt taagagggcg tacatccttg
    15301 tacacgtcgg gcaggaggga ttaattacca gcgatattca ttgcgggttt cccaatccac
    15361 gacctctttt tctgcctgat ctttctgata accataacgt tcctggattt taccgaccag
    15421 ttgatcacgt ttaccttcaa tgatcgtcat atcatcatcg gtcagtttgc cccattgctc
    15481 tttcacttta cctttaaact gtttccagtt accgccggct tcatctttat tcataatcaa
    15541 gacctcatcg ttaggttgtg aatgagagta cgttcacttt tcttctgaac gtgagattaa
    15601 gtgtagtcag caatttggct taggataatt tattcagaat ttttaaccgt cacgttgcgg
    15661 caaaccaggt accgttgcgc cagtgacgcc gccagatagc cgccagagaa agcccgcgca
    15721 acgccagaaa gacggttaat gccagccaca aaccatgatt acccagccac ggcagcgtaa
    15781 ggagcgtcag cgcaaaacct gcggcggcca ccgccatact gttacgcatt tcggcggcac
    15841 gcgttgcgcc tataaacatg ccgtccagca gataacacca gacgccaacc aacggcaaaa
    15901 tcacctgcca gataagatag cggtcagcca gctgctgaat ctgggttaac gacgtcagca
    15961 acgcaatgat gtgttcccct gccagcaaat aaaccaccga aaacagtaac gctacaatcc
    16021 ccgactggcg gcacgctgcc cgccatacat ccagcaactg gctaccgtcg cgcgcaccgt
    16081 atgcctgacc ggagtgcgct tcaaccgcgt aggcaaaacc atcaagcgca taggcggtaa
    16141 aggtgagtag cgtcatcaga accgcgttaa cagcgataat gtcactcccc agtcgcgcac
    16201 caagtacggt gatcgcgccg aaacagagtt gcaacaacag cgaacgcagc atgatatcgc
    16261 ggttaagcgc cagcaagcga cggaagtttc ctcgccaggc agttttcagc atttcgccgg
    16321 aaattccgcg tagtttgagg attttacgca ccattagcag accaatcagc aatgttgcat
    16381 attccgcaat aaccgtcgcc agcgccgcgc cctgcacgtt catatgcagc cccatcacca
    16441 gccagacatc cagcacaatg ttgaggatat tgccgaccac taacaaaatt actggcgcac
    16501 gggcatattg cacgccgagt aaccaaccga gtaataccag attcgccagc gacgccggag
    16561 cgcttaacca gcggatttca agaaagcgcc gcgcctgttc caggaccgct tcactaccgc
    16621 caacaatatg cagcgccaga tcgataatcg gcgtacgcaa cagcgcaatt aacgccccgg
    16681 cccccaacgc caacagcaac ggttgcacca gcgcacgggc taatgcctga ggatttttgg
    16741 caccataagc ctgcgcagtc agcccggtgg tgctcatgcg taaaaacagc aacagcataa
    16801 agagaaagct ggtcgccgtt gcaccaaccg ccacgccgcc caaataaacc ggactatcaa
    16861 gatgaccaat taccgccgta tcgaccagtc ccagcaacgg aacggtgata ttggagaaaa
    16921 tcatgggtaa ggcgagatgc cagagtgctt tatcagatga agtgaggaat gccatgcaga
    16981 caagcctgat gaagagagat gaaaaacaaa ccgcgacgcc aggcggcatc gcggtctcag
    17041 agatatgtta cagccagtcg ccgttgcgaa taaccccaac cgccagccct tcaatggtga
    17101 agctctgctg acgaaggtca acgacaattg gtttaaactc gctattttct ggcaacagtt
    17161 cgactttatt gccctgtttt ttcaggcgct taacggtaac ttcgtcatca atacgtgcga
    17221 caacgacctg accgttacgt acatcctgag ttttatgcac tgccagcaag tcgccatcca
    17281 taatgccgat atctttcatc gacatcccgc tgacgcgcag caggaaatca gcattcggct
    17341 tgaacaagga aggatcgacc tgataatgac cttcaatatg ctgttgcgcc agaagcggtt
    17401 caccggcagc cacacgacct accagcggca acccttcttc ctcttcctgc aacagacgaa
    17461 tcccgcgtga tgcgccggaa acaatttcaa taacgccttt gcgtgccagc gccttcagat
    17521 gttcttcagc cgcgtttggg gaacggaacc ccaaacgctg cgcgatttcc gcacgcgtcg
    17581 gcggcatacc tgtctggctg atgtgatcac ggatgagatc aaacacctct tgttgcctgg
    17641 ccgttaacgc tttcattccg ccccctgggt gtatatacag ttatactgtg agtatataca
    17701 gcaaaaggcg attttggaac cataaactgc acaataaacc agagatttat cgaaatctgg
    17761 aagggttatc caaaatgcga ccataacagg atgcaccagg taatcacggc gacgataatg
    17821 gcaatcagca ccgcggcgga tcccatatct tttgcgcgtc cggaaagctc atggtattca
    17881 gagccaattc ggtcaaccac tgcttcgatg gcgctattga ggatttccac aatcatcacc
    17941 agcatcacgg agctgataag cagcacgcgg gtaattgcgt ccacatccag ccagcaggcg
    18001 atgaccaccg ccaacaatac cgctacgcct tcctgacgga atgccgcttc gttgatccat
    18061 gcagcgcgta aacctttcca ggaatagcca gcagctttga taattcgggt gaatccagtg
    18121 gtattattgg ccattgaaag aaccttttta cattatgagc gtcaatatca gtgtaccgtt
    18181 aaatccgatg attcgggttt gcgtaacgcc gcgcgaaaca tgagcggata ccacagaatt
    18241 tcccatgact ttctgctatc cttgccgcgc atttgcatta ttaaccagag gctttacatc
    18301 gtttatgtcc ggctggccac gaatttacta caaattactg aatttaccat taagcatcct
    18361 ggtaaaaagc aagtctattc cggcagatcc tgccccggaa ctggggctgg atacctctcg
    18421 tccaattatg tacgttttac cgtacaactc gaaagcagat ttgctgacgt tgcgcgccca
    18481 gtgtctggca catgatttgc ctgacccgtt agagccgctg gaaatcgacg gcacgctact
    18541 gccgcgctat gtgttcattc acggcgggcc gcgtgtattc acctattaca cgccgaaaga
    18601 agagtctatt aagcttttcc acgactatct cgatttgcac cgtagcaacc caaatctgga
    18661 tgtgcagatg gtgccagtat cggtgatgtt tggtcgcgcg ccggggcgtg aaaaaggcga
    18721 agtgaacccg ccgctgcgta tgcttaacgg cgtacagaaa tttttcgctg tactgtggct
    18781 cggtcgcgac agttttgtgc gtttctcgcc gtcagtgtcg ctgcgccgta tggcggatga
    18841 acacggcacg gataaaacta tcgctcagaa actggcgcgc gtggcgcgta tgcactttgc
    18901 ccgtcaacgt ctggctgccg taggcccacg tctgcctgct cgtcaggatc tgtttaataa
    18961 gctgctcgcc tcccgcgcca ttgccaaagc ggtagaagat gaagcgcgca gcaaaaaaat
    19021 ctcccatgaa aaagcgcagc agaacgcgat tgcgctgatg gaagagattg cggcgaattt
    19081 ctcttacgag atgatccgcc tgactgaccg cattctgggc ttcacctgga accgatttta
    19141 tcagggcatc aacgtccata acgccgagcg cgttcgccag ctggcccacg acggtcatga
    19201 gctggtgtat gtgccttgcc accgcagtca catggactac ctgctacttt cttacgtgct
    19261 gtaccaccag gggctggtgc cgccgcatat cgccgccggg atcaacctga acttctggcc
    19321 ggccgggccg attttccgcc gtctgggggc gttctttatt cgccgtactt ttaaaggcaa
    19381 taaactttat tccaccgttt tccgtgagta tctcggcgaa ctgttcagcc gtggttattc
    19441 cgtcgagtac ttcgtggaag gcggtcgttc ccgcacgggg cgtttgctgg atccgaaaac
    19501 cggtacgctg tcgatgacca ttcaggcgat gctgcgtggc ggcacgcgtc cgattacgct
    19561 gattccgatc tatatcggtt atgagcacgt catggaagtg ggtacttacg ccaaagaact
    19621 gcgcggcgcg acgaaagaga aagagagcct gccgcagatg ctgcgcggtt taagcaagct
    19681 gcgtaatctc ggtcagggtt acgtcaactt cggtgaacca atgccgttga tgacctacct
    19741 taaccagcac gtaccagact ggcgtgaatc tatcgatccc atcgaagcgg tgcgtccggc
    19801 ctggttaacg ccgacggtca ataatattgc tgccgatctg atggtacgca ttaacaacgc
    19861 aggcgcggca aacgccatga acctgtgctg tactgcgcta ctggcatcac gtcagcgctc
    19921 actcacccgc gagcagttaa ccgagcaact caactgctac ctggatctga tgcgcaacgt
    19981 accttactcc acggactcta ccgttccttc agccagcgcc agcgagctta tcgatcacgc
    20041 gctgcaaatg aacaagtttg aagtcgagaa agacactatc ggcgacatca tcattctgcc
    20101 acgcgagcaa gcggtgctga tgacctacta tcgcaacaac attgcgcata tgttggtgct
    20161 gccttcgctg atggcggcaa ttgtcaccca gcatcgccac atctcccgcg acgtattgat
    20221 ggagcacgtc aatgtgcttt acccaatgct gaaagcggag ctgttcctgc gctgggatcg
    20281 cgacgagttg ccggacgtta ttgatgcgct ggcaaatgag atgcaacgtc aggggctgat
    20341 taccctgcaa gatgatgagt tgcatatcaa cccggcgcat tctcgcaccc tacagctgct
    20401 ggccgcaggc gcgcgcgaaa cgctgcaacg ttatgccatc accttctggt tgttgagtgc
    20461 caacccgtcg atcaaccgcg gtacgctgga gaaagagagc cgcaccgttg cgcaacgtct
    20521 ctccgtgctg cacggcatca acgcgccgga gttcttcgac aaggcggtgt tcagttctct
    20581 ggtgctgacg ctgcgtgatg aagggtatat cagcgatagc ggcgatgccg aaccagcaga
    20641 aacgatgaag gtttatcagt tgctggcgga gttgattaca tcagacgtgc gtttgacgat
    20701 tgagagtgcg acgcagggcg aagggtaatc agagagaatt gccggatgcg gcgaaaacgc
    20761 cttatccggc ctaccatgac ctgcaaattc aataaattgc gattcaccgg gtaggcctga
    20821 gaagcgcagc gcatcaggca acccagaaga aagccggatg atcatccggc ttttttacat
    20881 catcagaaat gccagtaact cattgccagt cctaaaaaca gtaccagacc aacatagtta
    20941 ttattcataa atgctttaaa gcaggcttca cgctcgcggt tggcaatcag tttttgttga
    21001 taaacaaaca gcgcgccagc caccacaatt gaccagtaat atccccagcc taagccattt
    21061 aactcaccga tgatcgccat cagtgccagt acgccaatct gcaaaatacc aataatcaat
    21121 ttatcgtatt ggccgaacag gattgccgtg gatttaatgc caatcttcac atcatcatcg
    21181 cggtcaacca tcgcatactg cgtgtcgtaa gccaccgccc agagaatatt ggcgaggaac
    21241 attaaccagc aactcaatgg caccgactca ctcacagcgg caaaagccat tggaatcgac
    21301 cagccaaacg ccgcgcccag caccacttgc gggagatggg tataccgctt cataaacggg
    21361 tacacccacg ccaatgccag agcggcaatc gacaacagaa tggtcatcgt attcagcgtc
    21421 agaaccagta aaaacgaaat cagtaccagc actacaaaca gcgcgcgcgc ctctttctct
    21481 gttaccgcgc cgctgggaag tggtcggttc gccgtgcgct taacatgacc atcaaacttg
    21541 cgatcagcat aatcattcac cacgcaacca gcagcgcgca tcagccagac acccgcgaca
    21601 aacaccgcca ggatccagag ctgaggaacg cccggtgtcg ccacccacaa cgcccataat
    21661 gttggccaga gcagcaataa cgcgccaatt ggcttatccg tacgcattaa gcgatgaaac
    21721 gccagcagct tattctgcgt cagactccac tccatatttt tttcctctta gtacaacggt
    21781 gacgccggta aaaacagttc tgttagcaac agcggtttac cgcttaatcg caggcgggaa
    21841 cgtcgccccc acagtccggc atcacggcct atctcgataa aatcccgggt tagtgtcgat
    21901 gatgtgaaca gatagcgtcc taacggcgtt ttacccaatt tttgtaacgc tagttccggc
    21961 ccgcttaatg ttgatacagg aacgacggta cgaccggcaa gccacggttc accatcggca
    22021 cataacaaaa tttcacgtaa ccagtaacga gactctttcg gcagtagcgg cagttcttcg
    22081 gggatttcat tctgctcgac aaacccctcg cggatcatcg tcacactcac cgtttttccc
    22141 tgctgttcaa aacgttttgt catggaatcc tccagcaaca gccagtcgaa cagttgcgga
    22201 tccagggcag ggatctcttt aaaatagcgc agcgcacgca gttgcgttaa cgcggggtgt
    22261 gacatgccga actctccgtt acataacgta aaggcattgt atcgcagaaa agtgtttacg
    22321 ggagatgtaa agcaaattaa gtgatctaat gcgcaacaaa aacgcaacaa tcgcatgaag
    22381 tggtaaaacg ctgttgaacc ttacgctttg ccgatgcccg tgctggcgag ggcaaccata
    22441 ggggcatcac cgcccatgat gctaggaacg gtgatgtttt cctttactta aataccttta
    22501 ttctttatac gagtagctcg ccaactcgca gtaaaaattg ggaaaagcgc atatttacca
    22561 tgagatttgg agctgaaaag tgtttcctga gtaagattaa taggcaacat cttatgtctg
    22621 gatttataga tacacgtgga attccaattg gtatcttttg taggattcaa catatcatgt
    22681 aagcgctggg agcttaccac cataacaggc ccatcatcga cagaggagaa caggataaat
    22741 atattatcgt ttcgtgttaa tttattagtg ttaaaggttc ttaacttttc ttcagtttct
    22801 ataaatatag gtgaaacatc atcactacga atccactctt tataaagata ccaggcattt
    22861 gctttgatca tatcatccgt tgatcgtaag gcctgatcga taagttggat aaaaaaattg
    22921 ttatttttat caaccagccc tttattcata ttaaatctgt taataaaatg gatcatctgt
    22981 tgggtatcga aattcacctt actgaatcga tgttgacgca taaaattatc gaacttgtca
    23041 tcttccccag aatgtatata actacgtata attggatctt ttatataagg acaatggcta
    23101 aatgttttct tcatattctc aataaagttt ttattaacaa tattgagaat ttttaattgc
    23161 atcaccagtt ttactgccag aacggatttc aatatcattg cattatctgg caatgtatca
    23221 atcattttta taatgttatt gatataacca tcgagatctt cattcccaaa tatatctgaa
    23281 taatcaatag ttaattccaa ttgttctgta atgcaatctt aaaaattccc tgtcgacatt
    23341 cgcacatgat caaacataac agctgtcaaa ttcgatttaa taaatgatat ttctttggca
    23401 gacagaaact ctgctctggt gaagtgcgaa gcgtaaagat aacaattatc gaagtttgaa
    23461 tttctaatct gacattcgcg aaaattacta tgaccaagtt gactctgcga gaagtcaact
    23521 gtatcaagaa tattttggct aaaatcaaca cattcgccac tcgttttgag aaaaatcaca
    23581 ttgtgtaaac gtcgacattt aaatatgaca ttatgaactg ttgccttagt aaaattgcaa
    23641 ttctcaattt ctttacattc gaaagttaca tgttgtagat ttgatgataa aaaagacacc
    23701 ttagaaataa cgtcaccttt aaaatgacaa tttgttaaat caaaatcaga caggtcgata
    23761 tacccctgcg catctttatc catcagaaaa ttacaagcgc attctatttt ttcatttctg
    23821 tagaatttta cacttatagt atcattacta attgagactt cacgtaacat atttattgca
    23881 tgattttttt cgttccgttg ttttatattt aaaaatatat cactgatata aataaagtta
    23941 tctctgttta aacctgtcac cccattgaaa ttacgtagta aatcaagatc tactatatta
    24001 tttccttgca ccacatcatg tttcatcaaa gagttattgc cgccagaatt tgtgatatga
    24061 cctatcataa aacctctgta atacagaaat aaaataacct ggaaaagtag cctgagcgtc
    24121 cagacactac cggcgaaagg aaaagaatct ctgataatgc attgaaataa tggacattct
    24181 tagtgaagaa gaagtgaagt actataaaaa atcgctcata tcccaggaag gaagcataaa
    24241 aaaaggtgcg ccaggagacg caccagttgt tgcaaaatca gcactgtggg gcgtaattac
    24301 cccttgcctt ttacactgct gataaaggtg gaacgggcag atgtcgatcc caggcgttca
    24361 gcttcatcaa gcagttttaa cgccttatca acatcacctt tcgcgacagc gtttttgatc
    24421 gcggtattaa aataactttc cgtgtcgttg agcatcggct cgcttttctt caccggcacc
    24481 ggagcaggtg cagccacagc tggtgccgcc gtgttgccta ccgtaaccgg agctggagcg
    24541 gaagaaccaa atagtggtcc taccaacacg ctggagctgg agttcgtttt gactttcagt
    24601 ttcagtaagc catcgctggt atgacgagca accggatcgg gaatatccgg gatcgagtta
    24661 ccgacgcctt tggcataggc tttagccggg tcgagcagtt gggtcgtctg ctggagatct
    24721 ttttccatgg taaagaccag aacataaagt ttttgctgcc ccaacgccgg tgtcaggcgc
    24781 ataacgcctt ccagccgatc tgcactcatc acgcctagtt cctggtaggt gaaataactg
    24841 ctggggaaga aggctgatgg ggtcatgttc tgatcaataa tcagcacgtt cggcgcaaaa
    24901 acgctggttt gtttgttcac ttcgctggtc agcgtcaggg tcagttcgcc aatgtttgcc
    24961 gggacgctgt acgcagcaac cggaccactg atgccgggaa cgctcagttg ttggccgccg
    25021 gtcgccagtt gggtggtctg ggttttagat tgatcgaccg gtgtccaggt gagttgttgc
    25081 agcgcggcag atggaatggc tggcgcgtcg ctggtgtttt gcggtacgta gttaacatcg
    25141 gcaaggctaa ttccaggcgc gcttgccagt aaccctgctg ataaacagag ggcgatgaga
    25201 cttttattca ttttcattgt tttcacctca aaatctggag ctcagcggta gccaggcaat
    25261 agcgcgctaa acccgataat cagaggggct ttcgcccctt tcagataatg acaacctgtt
    25321 tttatgccgg atgcggcgta aacgccttat ccggcccagg ttttgctatt accaccagat
    25381 ttccatctgg gcaccgaagg tccactcgtc gctgtcgcca cgaccgaagc tgccgccgtt
    25441 gaaatcagca ggaacggctt tgccgtagtt cgggttaacc ttgctatcgc cgttgtagtc
    25501 gtaaccccat ttctcatccc acttggcgta ggttgcgaag acacgaatag ccgggcgtga
    25561 ccagatgctg tcgccagcct gccattgttg tgcgagggta attttgtact gattgttctt
    25621 gtcgccggtg cgctgggatt cgacgttgtc gtagccgatt tccatcacgg tgctcatgat
    25681 tggcgtccac ttgtacatcg ggcgaatacc aacggtccac cacttggtgc cgttgtcgtt
    25741 atcccagttg atatcctggt acatacccac gtacatcatg tcccagttgt cgcccatgga
    25801 gatcgcaccg tggtcgagga tacgcagcat gtgaccgttg ttgttgatat tgtaggcaaa
    25861 tttttcgtta tcaaacgcga cgccagaacc ctgcgacaga cctttaccct gcgaggtcat
    25921 cgagtcagta gcgtactgaa caacaaactt gttaaagccc ttcaggacac tctgagtatg
    25981 ttcagcagtg aataaccagc cgtctttcga tgcgccatca accagacgat agttatcacg
    26041 caggttggca cgaccgtagt cgacacccag ttctaatgtg ccgcccgggt tgatttccat
    26101 ctgcgctaaa cgcacatcga aaacgtcgtt cgcggtttcg ttggtatagt cataaatatt
    26161 gttgctggca aaagaggaag aaccaccagc ttcagaggag cgggttgctg ccagagagag
    26221 tttaccgaag ccaacatcga tgttttccag accggcacca ggaccagaaa tatcccagta
    26281 gtagaagtcg atcatatgaa cgtcatgacg ttggtagaag cgcttacctg cccagatggt
    26341 ggaacctggc agccattcga tcaggttttt tccctgcacg tttgcttcac ggaaggccgg
    26401 atcggtagct tcccagtcat tctgttgcgc gacggaatag gccacgttag tgtcgaaata
    26461 gaagctctta tcgccctctt tccacacttc ctgacccaat tttaattcag cataagtttc
    26521 acattcgttg ccaagacggt atttactttg agcaccggta gtctggaaac actgttgttc
    26581 accgccgcta cctgtccagc caataccgga acgtgcatag ccgtggaaat caacagccat
    26641 tgcctgagca gacattacgc ccgctgcgac ggcaaccgcc agaggaagtt tgcgcagagt
    26701 aatcatcatt ctatctcctg agtcattgct tttctttttt cacatcacct gtgacaggct
    26761 ttgtgtgttt tgtggggtgc ttaaacaccc ggctccttat gcagtcgacg acatgcagtg
    26821 ccatcctcac ggaacagatg gcaacgctct ggcggcaggc cgatagcgaa tgtggcacct
    26881 tcttctacca acaccacgtc gttctggcgg tacaccaggt tttgacgaat ggaagggatc
    26941 tggatataga tttgggtttc gttgccgagt tgctcgacga cctgaacttc accctcaagg
    27001 atgacgtcag cgatatcact cggcagtaga tgttccgggc gaatacccag cgacatattg
    27061 gctccaacct ggacatcacg gctttcaaca ggcagccaga cttgttgacg atttggcatc
    27121 ggcagctcca cctgcacttg atcgattgcg gtggcggtca cttttaccgg caggaagttc
    27181 atctttggcg aaccgataaa tccggcgaca aaacggtctg ccggatagtg gtacagttcc
    27241 agcggtttcc caacctgtgc cacgcgaccg gcgtccagca ccacgatttt gtcggccagc
    27301 gtcatcgctt cgacctgatc gtgggtgacg taaatcattg tgcggcccag gcgtttatgc
    27361 agacgggaga tttcgatacg catttgcaca cgcagtgcag catcgaggtt ggagagcggt
    27421 tcatcgagca aaaatacgct tggctcggcc accagcgtac ggccaatcgc cacgcgctga
    27481 cgctgaccac cggagagcgc tttcggtttg cgatccagca aatgcgccag ttgcagcact
    27541 tccgccacct ggttgacgcg ttggttaatc acctcttttt ttgcgccagc cagtttcagg
    27601 ccaaatgaca tgttttctgc tactgacagg tggggataga gcgcgtaaga ctgaaacacc
    27661 ataccaacgc cgcgttctgc tggcggagtg tcattcatcc gtttctcacc gatgaacagg
    27721 tcgccgctgg tgatcgtctc aagcccggca atcatgcgca gtaaagtcga tttaccgcag
    27781 ccagacggtc cgacaaacac cacgaattca ccttcatgga tatcgagatt gatatctttc
    27841 gataccacga cctcgcccca ggctttcgtt acattttgca gctgtacgct cgccatgccc
    27901 ttctcccttt gtaacaacct gtcatcgaca gcaacattca tgatgggctg actatgcgtc
    27961 atcaggagat ggcttaaatc ctccaccccc tggctttttt atgggggagg aggcgggagg
    28021 atgagaacgc ggcttctgtg aactaaaccg aggtcatgta aggaatttcg tgatgttgct
    28081 tgcaaaaatc gtggcgattt tatgtgcgca tccccacatt accgccaatt ctgtaacaga
    28141 gatcacacaa agcaacggtg gggcgtaggg gcaaggagga tggaaagagg ttgccgtata
    28201 aagaaactag agtccgttta ggtgttttca cgagcacttc accaacaagg accatagatt
    28261 atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt
    28321 tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat
    28381 aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa
    28441 gtcaccgttg agcatccgga taaactggaa gagaaattcc ctcaggttgc ggcaactggc
    28501 gatggccctg acattatctt ctgggcgcac gaccgctttg gtggctacgc tcaatctggc
    28561 ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacatgg
    28621 gatgccgtac gttacaacgg caagctgatt gcttacccaa tcgctgttga agcgttatcg
    28681 ctgatttata acaaagacct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg
    28741 ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg
    28801 tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc
    28861 aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc
    28921 ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa
    28981 gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac
    29041 atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca
    29101 tctaaaccgt tcgttggcgt gctgagcacc ggtattaacg ccgccagtcc gaacaaagag
    29161 ctggcgaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat
    29221 aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat
    29281 ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg
    29341 cagatgtccg ctttctggta tgccgtacgt actgcggtga tcaacgccgc cagcggtcgt
    29401 cagactgtcg atgaagccct gaaagacgcg cagactcgta tcaccaagta atgctgtgaa
    29461 atgccggatg cggcgtgaac gccttgtccg gcctacaaaa ctgaaacgca tgtaggcctg
    29521 ataagacgca tcagtgtcgc atcaggcagt tgttgtcgga taaggcgtga aagccttatc
    29581 cgtcctggaa tgaggaagaa ccccatggat gtcattaaaa agaaacattg gtggcaaagc
    29641 gacgcgctga aatggtcagt gccaggtctg ctcggcctgc tggtgggtta ccttgttgtt
    29701 ttaatgtacg cacaagggga atacctgttc gccattacca cgctgatatt gagttcagcg
    29761 gggctgtata ttttcgccaa tcgtaaagcc tacgcctggc gctatgttta cccgggaatg
    29821 gctggaatgg gattattcgt cctcttccct ctggtctgca ccatcgccat tgccttcacc
    29881 aactacagca gcactaacca gctgactttt gaacgtgcgc aggaagtgtt gttagatcgc
    29941 tcctggcaag caggcaaaac ctataacttt ggtctttacc cggcgggcga tgagtggcaa
    30001 ctggcgctca gcgacggcga aaccggcaaa aattacctct ccgacgcgtt taaatttggc
    30061 ggcgagcaaa aactgcaact gaaagaaacg accgcccagc ccgaaggcga acgcgcgaat
    30121 ctgcgcgtga ttacccagaa tcgtcaggcg ctgagtgaca ttaccaccat tctgccggat
    30181 ggcaacaaag tgatgatgag ctccctgcgc cagttttctg gcacgcagcc gctctacaca
    30241 ctcgacggtg acggcacgtt gacgaataat cagagtggcg tgaaatatcg tccgaataac
    30301 caaattggct tttaccagtc cattaccgcc gacggcaact ggggggatga aaagctaagc
    30361 cccggttaca ccgtgaccac cggctggaaa aactttaccc gcgtctttac cgacgaaggc
    30421 attcagaaac cgttcctcgc cattttcgtc tggaccgtgg tgttctcgct gatcactgtc
    30481 tttttaacgg tggcggtcgg catggttctg gcatgtctgg tgcagtggga agcgttgcgc
    30541 ggcaaagcgg tctatcgcgt cctgctgatt ctgccctacg cggtgccatc gttcatttca
    30601 atcttgattt tcaaaggatt gtttaaccag agcttcggtg aaatcaacat gatgttgagc
    30661 gcgctgtttg gcgtgaagcc cgcctggttc agcgatccga ccaccgctcg cacgatgctg
    30721 attatcgtca atacctggct gggttatccg tacatgatga tcctctgcat gggcttgctg
    30781 aaagcgattc cggacgattt gtatgaagcc tcagcaatgg atggcgcagg tccgttccag
    30841 aacttcttta agattacgct gccgctgctg attaaaccgc tgacgccgct gatgatcgcc
    30901 agcttcgcct ttaactttaa caacttcgtg ctgattcagc tgttaaccaa cggcggcccg
    30961 gatcgtcttg gcacgaccac gccagccggt tataccgacc tgcttgttaa ctacacctac
    31021 cgtatcgctt ttgaaggcgg cgggggtcag gacttcggtc tggcggcagc aattgccacg
    31081 ctgatcttcc tgctggtggg tgcgctggcg atagtgaacc tgaaagccac gcgaatgaag
    31141 tttgattaag ggagataaca aaaatggcaa tggtccaacc gaaatcgcaa aaagcacgtt
    31201 tgtttattac tcacctgcta ctgctacttt ttatcgcggc gattatgttc ccgctgctga
    31261 tggtcgtcgc tatctcgctg cgtcagggaa acttcgcgac cggtagcctg atcccggagc
    31321 aaatctcctg ggatcactgg aaactggcgt taggttttag cgttgaacag gctgatggtc
    31381 gcattacgcc accgccattc ccggtactgc tgtggctgtg gaactcggta aaggtcgctg
    31441 ggatttccgc gattggcatt gtggcgctct ccaccacctg cgcctacgct ttcgcccgta
    31501 tgcgctttcc aggcaaagcg acgctgctga aaggaatgct gattttccag atgttcccgg
    31561 cagtactttc actggtcgcg ttgtatgcgt tgtttgatcg tctgggtgag tacattccat
    31621 tcattggcct gaatactcac ggcggcgtaa ttttcgcgta tctgggtggg attgcgctgc
    31681 atgtctggac catcaaaggc tatttcgaaa ccatcgacag ttcgctggaa gaagcggctg
    31741 cgctggatgg tgcgacaccc tggcaggcct tccgccttgt cctgttgccg ctgtcagtac
    31801 cgattctggc ggtggtattc atcctgtcgt ttatcgctgc cattactgaa gttccggtcg
    31861 cgtcgctgtt actgcgtgac gtaaacagct acaccctggc cgtggggatg cagcaatacc
    31921 tcaacccgca aaactacctg tggggtgact ttgccgccgc tgccgtgatg tctgcattac
    31981 cgatcaccat cgtcttcttg ctggctcaac gctggctggt caacggcctg acggcaggtg
    32041 gtgtgaaagg ttaaagatgt tgttctgcca atgttatgcc gctgcaccct caacttacgt
    32101 tatcccaact tgtgactatt attcggcgct ccacggagcg ccttttttct ttcgtctgca
    32161 atctgaatcg ttcgccggtt aatattccca tcatagaact tattattttt acgttatttg
    32221 ttttcccact tacgataatt ctctttcgtg ctctgagtca cggcaatagt attgttttca
    32281 tcaatttttg ataattatca caattaagat cacagaaaag acattacg
//
LOCUS       DADLTF010000032        32306 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.032, whole genome shotgun sequence.
ACCESSION   DADLTF010000032 DADLTF010000000
VERSION     DADLTF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.032"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            63..695
                     /locus_tag="JN749_001395"
     CDS             63..695
                     /locus_tag="JN749_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7367864.1"
                     /translation="MKNLPIGGVWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFP
                     EFAHATPRVQLDLHPTGSVNGSNYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAA
                     GRRDGYFSIYRQGGTTTDEGERIDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRP
                     VVLPGIRYAVMCVPTPLTLAVDKFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(783..2081)
                     /locus_tag="JN749_001396"
     CDS             complement(783..2081)
                     /locus_tag="JN749_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7367865.1"
                     /translation="MSKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            2564..3976
                     /gene="uxaC"
                     /locus_tag="JN749_001397"
     CDS             2564..3976
                     /gene="uxaC"
                     /locus_tag="JN749_001397"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAY7367866.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHFELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            3991..5478
                     /locus_tag="JN749_001398"
     CDS             3991..5478
                     /locus_tag="JN749_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAY7367867.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            5561..6112
                     /locus_tag="JN749_001399"
     CDS             5561..6112
                     /locus_tag="JN749_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HAY7367868.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="JN749_001400"
     CDS             complement(6117..7361)
                     /gene="sstT"
                     /locus_tag="JN749_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAY7367869.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(7957..8922)
                     /gene="alx"
                     /locus_tag="JN749_001401"
     CDS             complement(7957..8922)
                     /gene="alx"
                     /locus_tag="JN749_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HAY7367870.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(9193..9393)
                     /locus_tag="JN749_001402"
     CDS             complement(9193..9393)
                     /locus_tag="JN749_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7367871.1"
                     /translation="MQDLTQPQHINTMLYEAELFATLVDEHLVDHPGLAVSRITAKLL
                     TEIRRQTGVIFPADSVKLYLPK"
     gene            complement(9424..10191)
                     /locus_tag="JN749_001403"
     CDS             complement(9424..10191)
                     /locus_tag="JN749_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAY7367872.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSEC"
     gene            complement(10270..10962)
                     /locus_tag="JN749_001404"
     CDS             complement(10270..10962)
                     /locus_tag="JN749_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HAY7367873.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(11039..11542)
                     /locus_tag="JN749_001405"
     CDS             complement(11039..11542)
                     /locus_tag="JN749_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7367874.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            11627..12763
                     /gene="rlmG"
                     /locus_tag="JN749_001406"
     CDS             11627..12763
                     /gene="rlmG"
                     /locus_tag="JN749_001406"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HAY7367875.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            13048..13362
                     /locus_tag="JN749_001407"
     CDS             13048..13362
                     /locus_tag="JN749_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase HigB"
                     /protein_id="HAY7367876.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            13359..13775
                     /gene="higA"
                     /locus_tag="JN749_001408"
     CDS             13359..13775
                     /gene="higA"
                     /locus_tag="JN749_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HAY7367877.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(13820..15838)
                     /locus_tag="JN749_001409"
     CDS             complement(13820..15838)
                     /locus_tag="JN749_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HAY7367878.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="JN749_001410"
                     /pseudo
     CDS             complement(16058..18409)
                     /gene="ygjK"
                     /locus_tag="JN749_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(18426..19497)
                     /gene="ygjJ"
                     /locus_tag="JN749_001411"
                     /pseudo
     CDS             complement(18426..19497)
                     /gene="ygjJ"
                     /locus_tag="JN749_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(19631..21064)
                     /locus_tag="JN749_001412"
     CDS             complement(19631..21064)
                     /locus_tag="JN749_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7367879.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(21127..21576)
                     /locus_tag="JN749_001413"
     CDS             complement(21127..21576)
                     /locus_tag="JN749_001413"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HAY7367880.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATHALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="JN749_001414"
     CDS             complement(21573..24659)
                     /gene="ebgA"
                     /locus_tag="JN749_001414"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HAY7367881.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="JN749_001415"
     CDS             complement(24842..25825)
                     /gene="ebgR"
                     /locus_tag="JN749_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HAY7367882.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            26044..26375
                     /locus_tag="JN749_001416"
                     /pseudo
     CDS             26044..26375
                     /locus_tag="JN749_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(26417..27907)
                     /gene="ygjG"
                     /locus_tag="JN749_001417"
     CDS             complement(26417..27907)
                     /gene="ygjG"
                     /locus_tag="JN749_001417"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HAY7367883.1"
                     /translation="MITAFVFIPIFAIAAGVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            28213..29733
                     /locus_tag="JN749_001418"
     CDS             28213..29733
                     /locus_tag="JN749_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7367884.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(29887..30510)
                     /locus_tag="JN749_001419"
     CDS             complement(29887..30510)
                     /locus_tag="JN749_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAY7367885.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            30787..31533
                     /gene="nfeF"
                     /locus_tag="JN749_001420"
                     /pseudo
     CDS             30787..31533
                     /gene="nfeF"
                     /locus_tag="JN749_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(31587..31662)
                     /locus_tag="JN749_001421"
     tRNA            complement(31587..31662)
                     /locus_tag="JN749_001421"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31627..31629),aa:Ile,seq:cat)
     gene            31787..32293
                     /gene="mug"
                     /locus_tag="JN749_001422"
     CDS             31787..32293
                     /gene="mug"
                     /locus_tag="JN749_001422"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HAY7367886.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         7815 a         8429 c         8500 g         7562 t
ORIGIN      
        1 atggtcaaca ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag
       61 agatgaagaa tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca
      121 gcccggccca ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg
      181 acgtgttctt cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa
      241 caggaagcgt taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt
      301 acgatggttt taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac
      361 cagctgcagg gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg
      421 acgagggaga acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa
      481 ttgatgtgcg caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc
      541 cggtggtact gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg
      601 cagtcgataa attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag
      661 taatctttac gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt
      721 ttctatataa acgacgaagc cgcctgataa ggcggcttcg tcgttataac ttacgttaaa
      781 cgttaatgtt gcggtgcggg atcgttatgt gtttcctgtg ccacctcaat cgccggtttg
      841 ttctgcaata cggtccagat aaccagcgca cccagcaggt caaatactgc cagaactgcg
      901 aacagcgggc tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta
      961 cttgccagcc atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg
     1021 aacacgtcag aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg
     1081 atacacagca gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca
     1141 atcatcagca cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca
     1201 aaccaacgct ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac
     1261 agcatcggca tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac
     1321 atgaacagcg ggatccacgc gttaaaagta ccccaggccg gttctgctag gaaacgcggc
     1381 agtgcgatac cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc
     1441 gtgctcacct ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc
     1501 agatgcttct ggtcgcgcgg atgtttatag aaaatcagcc atgccatcgc ccagataaag
     1561 ctcaatgcac cggagatgat aaatgccatc tgccagctgt gcatcacgat tgcccatacc
     1621 accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta gcctactgcg
     1681 atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc
     1741 gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca gctacctgcc
     1801 agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata accgattttc
     1861 gtacccagca catccagtac ataacctgct accggttgca tgaccgtata agcagcagaa
     1921 taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt
     1981 ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc gagcgtcacc
     2041 agtgcgatca tataccaacg taacccttta attttactca tgtaaaacct catcgttttg
     2101 ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc
     2161 gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc
     2221 ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc
     2281 cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacctt aaaaggtgag
     2341 agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac
     2401 tggcgcggcc tgcagcgaga tttaccactt tgagagtaat ttttttaact acgtttattg
     2461 atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc
     2521 gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac
     2581 tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga
     2641 ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg
     2701 ttttaaaaac ctgtatgaca tctggttgaa gggcgatcac tacaaatggc gcgctatgcg
     2761 taccaacggt gtggctgagc gtctgtgtac tggtgatgcg tctgaccgtg aaaaatttga
     2821 cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcactt
     2881 cgaactgcgt cgtccgttcg gtatcactgg caaattgctt tctccgtcaa ctgccgatga
     2941 aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat
     3001 gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca
     3061 tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc
     3121 ggacaaagcc ttcaatatcg aacaggcgac ctttaacgac tacatggcga agctgggcga
     3181 agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct
     3241 ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt
     3301 tgctgaagcg aacgaagcgg aactcgacag cattctggcg cgccgtctgg ctggcgaaac
     3361 cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga
     3421 atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct
     3481 gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat
     3541 ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac
     3601 cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt
     3661 ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca
     3721 gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgttt
     3781 tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg
     3841 ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat
     3901 caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc
     3961 cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat
     4021 aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag
     4081 actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca
     4141 aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg
     4201 gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat
     4261 cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcctga agtgcagatc
     4321 tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct gccaaccgtg
     4381 ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac
     4441 gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc
     4501 gacgatcaca tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc
     4561 gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg
     4621 ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag
     4681 atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc
     4741 gagccaggaa aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt
     4801 tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc
     4861 ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac
     4921 cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag
     4981 tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa caaagcgggc
     5041 ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg ttccagtgtc
     5101 gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc cagggctgaa cttgttaagt
     5161 gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg
     5221 ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt gaaaatcgcc
     5281 accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg
     5341 atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgagttt
     5401 gccaacggta agcaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa
     5461 agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt
     5521 gaagcgccgt ttgcttttcc atacctgcta aggatttatc atgaccgcgt attggctggc
     5581 ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga
     5641 acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct
     5701 tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac
     5761 cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg
     5821 cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac
     5881 ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg
     5941 ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacgatga ttgagggtag
     6001 ttttctgctt atgaatggcc tgaatatcat tcgtttctgg cggatgcaga aaaggggaat
     6061 tgatccgttt aaagtagaga aaacccctcc cgccatagac gaaaggggtt aaacaattaa
     6121 ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac
     6181 agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgataata
     6241 aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc
     6301 agtgggatca gcagcaaaga accccccgcc acgccggatg cgccacaggc acacagagaa
     6361 gccactacgc tcaacagcag cgctgtgggc agatcgaccg gaatacccag cgtattaacc
     6421 gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat ggtggctccc
     6481 agcggaatag aaactgaata ggtatcgcga tccagattca gcttttcaca cagcgccata
     6541 ttcaccggaa tgttcgctgc agagctgcgg gtgaagaagg catacacacc gctttcgcgc
     6601 aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg
     6661 ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag
     6721 ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagccc aaaaataccg
     6781 atcggtgcga agcgaatgac cagtttcacc ataaaggtaa cggcattcga catatcattg
     6841 accaggtttt tggtggtctc gttaccgtga cgcagtgcga agccgaggcc gatcgcccac
     6901 accagaatcc cgatgtagtt acctttcagc agggcgtcga tggggttgga aaccatgctc
     6961 attaccagtc cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta
     7021 ctggacaagt gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagctgca
     7081 gagaaggtgc ccagcagata gaggaacaaa ataggacgga tattggtttt ctgcccgtgc
     7141 tggtggttag caatagatgc cattaccagc atcaacacca ggatgggggc aacagctttc
     7201 agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc
     7261 catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga
     7321 gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt
     7381 gttgtcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt
     7441 gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag
     7501 agaggtcatg ttttgcaact taatggttgc atttatttaa cataaacaga gggaaggact
     7561 ggctattaag gctgattaga taacatatgg gcaaaactat ttggaatatg agaacggttg
     7621 cgctccttta acgtgttatg ccatataaag tcactggcgc agggagcgcg cagggggcgg
     7681 ccaatcgccg ccgccccctg cccccccagg ctctggcgaa caaaatcgcc gctgcgcggt
     7741 tccctcagcc tatcccttgc ggctaccggg tcgggcgcaa ggtaacgtcc ctgtaagatg
     7801 cgccctcgcc ccggcctcca gggaacgctt cggcgatttt gacgccacca acacccgtgc
     7861 ggtctttttg taaagagcta taagccgatt acataattaa tagctcacct ttgtctggaa
     7921 tgagttatct ctaggaatgg cttaatatac agcgaatcat tctccccgct gcttatcatg
     7981 ccgataattc acccaggcgt tgataataaa cgtcatcacc agaatgccaa acaccacgcc
     8041 cagcgagacg gcgattggaa tatggtagaa gtcgacaatc agcatcttga taccgataaa
     8101 caccagaatc accgccaggc catatttgag catcgagaaa cgctctgcta cgcccgccag
     8161 caggaaatac atcgcacgca ggccgaggat tgcaaacagg tttgaggtca gcacaatgaa
     8221 cgggtcagtg gtcacggcga agatagccgg aatgctatcc acggcaaaaa tcacgtcgct
     8281 caattccacc agaatcagta ccagcatcag cggtgtggcg tacagcaatc cattcttacg
     8341 cacaaagaaa tgctcgttgt cgatggtgtc ggtcatgcgc aaatgaccgc gtagccagcg
     8401 caccagcggc ttgtcgccaa tgcctgattc atcttcatgg gcgagcgcca tcttcacgcc
     8461 ggtaaacagc aggaaggcac cgaagatata caggatccag tcgaactgcg aaatcagcca
     8521 gctgcctgtg aagatcatga tggtacgcag aacaatcgcc ccgagcacgc catacaccag
     8581 cacgcgacgt tgtaatgccg ccggaacgga gaaatagctg aacaacatca gccagacaaa
     8641 gacgttatcg accgccagcg atttctcaat cagataaccc gtgagaaagg ccagggcctg
     8701 tggatcggca acggcgcgac cttcggtttg caccagatac caccagaaag cggcgttaaa
     8761 cagtaacgac agcgtcaccc agaccagcga ccaggccgca gcctgtttca tggtcatggc
     8821 atgtgcccca cgacgcccct gcaacaacag gtcgatagcc agcataatgg cgacaacaac
     8881 agcaaatccg ccccatagca acggcgtgcc gacagtattc atagaagttc cttacacata
     8941 aaaaaacggc caacgtcgga agacgtcagc cgctgctttt tatgcataga cctcgccttc
     9001 cggcaaggtc tcacttacaa caaaaaagga atacgtctgc gtattccttt cgggttgccc
     9061 ggtgaccgga tgtggttttt cacacatcgt aatgacgatc gaccggcaat gaagttactc
     9121 ccctttgcag gtaacaaagt atgacagacc attcagtcag tcaatggtct gttacatacc
     9181 ttttacactg ttttactttg gcaagtatag ttttacgcta tctgccggaa aaatcacccc
     9241 agtctggcgg cggatctcgg tcagcagttt ggctgtgatg cgactgaccg ccagccctgg
     9301 atgatccacc agatgctcat ccaccagggt agcgaacagc tctgcttcgt agagcatggt
     9361 attaatatgc tgcggctggg tgagatcctg catctggctg ccacgcggca caaagcagac
     9421 tttctagcat tcagacagtt tttcaatcac cagcgatccc gcttcgccct gaatctcgct
     9481 tcccaggaca gaatcactga cctttgagtg ctgcaaggtg acgctgaaat caccgtaatc
     9541 catcaccacc acgccgtggg cgtcaacgcc gcttgccagc aaactcgccg ttgcctgcac
     9601 gcttttcggc tcaccaaata acgccaccgc cgacgccagg cagtaaaagc cgatatccat
     9661 aattgaaccg ttagagaatg ccggattaaa ggtgttggga ttctcaccgt ccaggtaacg
     9721 ttgataccgc gaggaatact ggcaatagtt gaaaaagact ttacgcagtt tgcctgcttt
     9781 cggcagcgcc tggcgcaaca aatgaaagtt cggcaggcag gcggttttaa atgcctcaaa
     9841 cagcaccacc tgattttcgc gcgcacaggc aatggcggca tccacttccg ccagattcga
     9901 cgccagcggt ttttcgcaaa tcacatgaat tttatggcta aggaaaagtt gtgtctggga
     9961 aaagtgcagg gaattcgggc tggcaatata caccgcgtca atggcatcgc tttccgccat
    10021 cgcttccagc gacgtaaaca gatgctcgac agaaaaatca ttggcgaagt gctgagcctg
    10081 ctcaaggctg cgggaatata cggcggttaa cttgtattta ccgctctcat gggcggcctc
    10141 gacgaactgg cgagtgatcc agttcgtacc aatcacagcg aaacgtatca taacgcgtgg
    10201 atactccata aagggattct gtcgtcaatg tagcacgcct ttttcatctt cgacgtgcgc
    10261 agatacgcat tattctttcg ccggacagcc atgctcacta aacggcccaa tcatgaccga
    10321 tggcccgaga tatttcggct ggcgatggag gatttttgcg tcaatcaccg cgctgacacg
    10381 agcgagtttc tcccgcagtt gagtataaaa accatgcttc atattgaggt ctggcgcgtt
    10441 aaacccgata gcgtcgatgc cgtactgttt tgccagccag atggcgcgtt ggttatgaaa
    10501 ttcttgcgag ataatggtga tatggttttc cccaaacacc tttttggcac gcactaccga
    10561 atccagcgtt gagaatccgg catagtcgca aaagatcact ttcgccggta cgcctttagc
    10621 gatcaaagcc tgctgcatac ctgatgcttc atcgtaattt ttacgtccgt tatcgccact
    10681 caccagtagc catttcactt ttccggcgtg gtacaacgct gctgcggtat caatacgtcg
    10741 ggtaaagtag cgattacccg gcctcgcccc cagcaataaa cccacgttac gcgccgggac
    10801 ggcgttgaca tcgccccagg tcagctgctt gctggcgttg accataacgc gatcggcgat
    10861 aaaaatggtc gctcctgcca caagtaacag taagcaggca atctttagag tccggcgaga
    10921 aaaacaaata cggagaagaa ggcgggcaaa tgcgcgcagc atggtcgtta tccttaacga
    10981 aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga catcattttc
    11041 agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg aactcaagct
    11101 ggtggtactg cggctccata tggcagcaca attgatagaa cgctttgtta tgctctttct
    11161 ccttcaggtg cgccagctcg tgcaccacga tcatgcgcaa aaacggttcc ggcgcgttgc
    11221 gaaacacggt agcgacgcgg atctctgctt ttgctttcag cttgccgccc tgcatacgag
    11281 aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg tcatacatca
    11341 ctttattgat cggcggggca ttacgcagaa actgattttt cagatcctga gtatattgcc
    11401 agagagcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc
    11461 ccagacgctg ttcgttaatc agtgttcgca cctgcgaaag cagctgctcg ggataaccct
    11521 gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt atactcacgc
    11581 acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga gccacttaga
    11641 caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg ttaacccgct
    11701 acaggcgtgg gaagcggcag atgaatattt gctgcaacag ttggacgaca cagaaatccc
    11761 cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca
    11821 taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg agaatttacg
    11881 cctaaacggg attgatgagt cgagcgtgaa gtttctcgac agcaccgccg actacccgca
    11941 acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg aacagcaact
    12001 gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg ccaaagcccg
    12061 tgacattcac acttccacgc tggaactgtt cgaaaaagtg ctcggcccga ccaccaccac
    12121 gctggcatgg aaaaaagcgc gcctgattaa ttgcacgttc aatgaaccgc cgctggccga
    12181 tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc ataaccatgc
    12241 gaatgtcttc tcccgcaccg ggcttgatat cggcgcgcgc ttctttatgc aacatctgcc
    12301 agagaatctc gaaggggaga ttgtcgatct cggttgcggt aatggcgtta ttggtctgac
    12361 gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt
    12421 tgcttccagc cgtttgaacg ttgaaaccaa catgccagag gcgttggatc gcagcgagtt
    12481 tatgatcaac aacgcgctct ccggcgtgga gcctttccgc tttaatgctg tgctctgcaa
    12541 ccctccgttt caccaacagc atgcgctgac cgataacgtc gcctgggaga tgttccacca
    12601 cgcccgccgc tgcctgaaaa tcaacggcga gctgtatatc gttgccaacc gccatctgga
    12661 ttacttccat aaactgaaga aaattttcgg caactgcacc actattgcga cgaataataa
    12721 atttgtggtg ctgaaagcgg tgaagctggg acgtcgtcgg taagattggg agttacctgc
    12781 cggatgcggc gtaaacgcct tatccggcct acgttcagca cgttaccccc aggccggata
    12841 agacgcggca agcgtcgcat ccggcaatgt ctgctcagtt ctggaatcgc gcttccataa
    12901 aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttttt ctacgtttat
    12961 tcttccgtca cacagataaa ttcatccgtt gccaatctgt caacggatgt tatcatattg
    13021 ccaatttgtc aacggacgtg atgaagaatg cacctgataa ctcaaaaagc attgaaagat
    13081 gctgcggaaa aatacccgca acataaaacg gagttggtgg ctctggggaa cacgattgct
    13141 aagggatatt tcaaaaaacc tgagtcatta aaagcagtat tcccatctct ggataacttc
    13201 aaatatctgg ataagcatta tgttttcaat gttgggggca atgaattacg tgttgtagca
    13261 atggtctttt ttgaatcgca aaagtgctac atacgtgaag ttatgacgca taaagaatac
    13321 gatttcttta ccgctgttca tcgtactaag gggaaaaaat gattgcgatt gccgacatct
    13381 tgcaagcagg agaaaagcta actgctgtgg caccttttct ggcgggtatt cagaacgagg
    13441 aacaatacac ccaggcgctg gaactggtag atcatctgct gctcaacgat cctgaaaacc
    13501 ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga agaatcagcg cccgaatttg
    13561 cggaatttaa tgccatggct caagccatgc ctggcggtat cgccgtgatt cgtaccctta
    13621 tggatcaata tggtttaacc ctttccgatc tgccggaaat tggcagtaaa tctatggtgt
    13681 cacgcgtttt gagcgggaag aggaaattaa cgctggaaca cgctaaaaaa ttggcaacgc
    13741 gattcggcat ttctcccgcc ttgtttattg attaacgcga cgtgccggat gcgttgcatc
    13801 cggcgcttca aataacgatt taaatctcca gcgccagccg tgttccctgg gcaattgccc
    13861 gtcgtgcgtc cagctccata gccacatcgc agccgccaat taaatgcaca gttttcccgc
    13921 tatcaatcag cggttgcgcc agcgcgcggt ttggctcttg ccctgcgcag atcaccacat
    13981 tgtccactgc taatacctgc gtttcgccgt tgatcaccac atgcagcccg tcatcgtcaa
    14041 tcttctgata acttacgcct gggatcattt tcacaccccg cgagagcagg gtggtgcgat
    14101 ggatccagcc ggtggttttg cctaacccct gtcccggttt gctggctttg cgctggagca
    14161 tcacaatttg ccgtgggcta cgcgggatct gcattccctg cgggcttaag ccaccagcct
    14221 gttgtaggct gctgtcgatc ccccactcat tacagaacct ggcgatattc tggctggtgg
    14281 attcgcccgg ctgacttaaa tacatcgccg tatcaaaacc aatcccgcca caaccgatga
    14341 tggcaacctt gttgcctacc ggcgctttgt cgcgcagtac atcgagataa ctcaatacct
    14401 tcggatgatc gatcccgtcg ataggcggaa cacgcggcac gatcccactg gcgaggatcg
    14461 tttcatcgaa cgcctgtaac tgatccgccg tcacggtgtg attgagtttc agcgtcacgc
    14521 ccgtcacttc gatcatccgg cggtagtagc gcagcgtttc gtaaaactcc tctttgccgg
    14581 ggatctgttt ggcgatatta aactgcccgc cgatctcgct gtgcgcatca aacaatgtta
    14641 cctgatgccc acgcgccgcc gcgttaatgg caaacgccag cccagcaggt ccagcgccga
    14701 ccaccgccag attttttttc tgcacggcgg gaaggattgg cattttggtt tcgtggcagg
    14761 cgcgaggatt caccaggcac gaggtgactt tgccaacgaa gatctgatcg aggcaggcct
    14821 ggttgcagcc aatacaagtg ttgatctcat cggctcgccc tgattgcgct tttgacagca
    14881 gttccgcatc cgcgagaaac ggtcgcgcca tcgatactat atcggcatcg ccgcgcgaga
    14941 gaatatcgtc ggcaacctgc ggatcgttaa tccggttggt ggttaccagc ggcagcgaga
    15001 cgtggccttt cagtttgcgc gtgacccagc taaatgcgcc gcgcggcacg ggcgtggcga
    15061 tggtcggaat ccgcgcttcg tgccagccta ttccggtgtt gataatggtc gcgcccgccg
    15121 cttcaatggc ctgcgccagt tctaccgttt cggcaaaagt gccgccgcct tcgaccaggt
    15181 cgagcatcga caaccggtaa ataataatga agtcgttgcc gacgcgttcg cgcaccgtac
    15241 gcactacttc tacggcaaat cgcatccggt tgcggtaatc gccgccccac tggtcactac
    15301 gctgattggt gcgcagcgtc agaaattcgt tgatcaaata cccttcagag cccatcactt
    15361 cgacgccatc gtatccggcc tcccgcgcca gttgcgcgca gcgggcgaaa tcgtcgatca
    15421 gttgcaggat ctcttcatgg gtgagttcgt gaggaacgaa acggttgatg ggggcctgca
    15481 atgcggacgg tgcgaccaga tgcggttggt agctgtagcg cccggtatgc aaaatttgca
    15541 gggcgatttt gccgccttcc tgatgtacgg cttcggtaat ggtgcgatgg tgtgggatct
    15601 ggctggcgtc gttgagcatt gcgccacctt ccatgccaac gcctattaaa tctggtgcaa
    15661 tgccgccgct gacaatcagc gccacgccgt gacgggcgcg ttcggcataa aacgcagcca
    15721 gccgttcagc accgtccggg tattcctcca gcccggtgtg cattgaaccc atcaacacgc
    15781 ggttttttaa cgtggtaaaa cctaaatcca gcggggcgaa cagcgacggg tagctcataa
    15841 aattgtccag tatgtaaaat aattgttacg gatgagttct aatttagccg tcgatggaga
    15901 aaagggaaaa ggggagtgcg gggtttgtga tgggattcaa aaagaatacg cccggtggta
    15961 acgccggatg cgacgcttgc cgtgttttat ccggcttggg ggattgcaca tgtagggcgg
    16021 ataaggcgtt cacgccgcat ccgccagccg ttgcaaatta ctgcttacgg aaaaaatcgt
    16081 tatacaacat atacaaatga gcggcactcc aggagaaatt tggtgcgcct tgctgcgcgc
    16141 ctgtcagcgg gttgtaattc tcctgaattg ggccatcggc ggttaatcct ttggcgtgcc
    16201 tgaagaacgt atccgccagc ttcagggcat catcacgata accgtaacgc tccatccctt
    16261 tcagaccaaa ccagaactga tccacccata cgcgcccgcg ccagtagata tcagcgccaa
    16321 aagccggatt ggttaacgcc gccgttccca gcggaacaaa ggtgttgaac tctttaggat
    16381 cgagcatcac cttcaccacc gcgtcggcat tggcctgcgt tgccgcaccg ttaaacagcg
    16441 gcgaccagcc ttccggccct ttaccgcgct caacaatcgg tttgcccgcg cagccgttcg
    16501 ccagtggttt atcttcaata cgtacgtcat agtagaactg ggtagtcggg tcgaacatac
    16561 aggtgttgat gtagtccgcg agctgctgtg ccaactggcg atagcgtttg gcctcttctg
    16621 gcttaccgag gatggttgcc atctccgcca gataatggtt atcgctgtac atatagctgg
    16681 cctgatccac cgactcctgc aatagcgagt agcccagcaa cgttccgtcc tgactgcggt
    16741 tttcggcgaa tttcaccgtc tagtcgctac gtttgccgcc attagcgaca tatttatcca
    16801 actgttcttt gtcgataaaa ccaaagacgg cggcatcatc acgccccgat tcccacgacg
    16861 cagcgacctg tgccggaatt tccagactgt cgtactggcc tttctccacc acgcgggcgt
    16921 agttgttcag cccagactgc gtctcttctt tatcgccttt tttcaccgta aacagcatct
    16981 cgccgctctc ggtgttgtgg gctttgtcgc gggtcgcgcc atattccggc accccgttgc
    17041 cgttatgatc gcggttacgt aaccaccagt cgtgataggc caccagtttc gggtacatct
    17101 ctgccaacca ggctttatcc tgggtgacgt tgtatacttc catcaccgac caggcggcaa
    17161 ggctgggttt ggtattacgt tcgttccagt tgccgccatc gccaccacgc tcggggctaa
    17221 ggttccacgc tatcaggtcg gggacaaagc ccacatcctg cggacgcacg ctgtcgccag
    17281 gctggatctg ccaggagaag accgcgcgga tattctcttt ggcgatatcc ggattgaaat
    17341 gcgccatcgc aaacgcctgc ttccaggtat cccacggcca ggtctgattg ccggagaacc
    17401 agcgtccggt caccgacggc gtgacggtgt tataattcac cgcaccgccc ggtgagcgcc
    17461 agttaccgtt gagcgtttcg atcgctttca ccgcgacgcg cgtctgttcc ggcgtcgcat
    17521 ccggattggt taaccctttc ttcaggtatt cttcccagcg ttgctgcgag gcggtgagat
    17581 aaaacgccgg acgcgccaga atatcgcgga tctgcatttg ctctttgcta acttcctgag
    17641 cggtcagcag atgggaatag gtggtgtaga gcgtggtcga accgttgata tgcgccttac
    17701 tggtaaagcg attaccgttg atttcagtct gcaccggcag ggatttatgc acctgatatt
    17761 ccgattcgcc ggaggtcagc agatcccagg tggcgcgcac tttgccaaag gtgactttca
    17821 ggccatcacg ggtggcgctg attttgcgct gatagtcagg gtattcgcca gcaatggttt
    17881 tatcggaaag cggtttccct tctttcgctt ccagtttttc cagcagttcg ccatcccaca
    17941 ccagatccag cggtttattg ctgatgattt tagtttccag tagtgacgtg cgcggcgtgg
    18001 cgaagcgcag agtcatttcg acctgcacat cttttgctgt cagtttttgc accagcgcgc
    18061 cgggaatact gtatgcctcc agcgtgaagt tgactttctt gccgtcttgc cagacagtca
    18121 ggcggtcgaa attgctggcc ataaagttga tgtattcttc cgtcagcagc gcaacgcccg
    18181 gaaagccgcc catggtgttc gggccgtctg gcaacagatg accatgccag gctccgagat
    18241 cgaaaaacgg attaaaacgc tggtgatcgt cgtaatcgta atccttcatg tactgcggcg
    18301 caccagtacg gttaatcacg tttttataat tgtcggcgtt agcggcatga gcggaaaaac
    18361 ttatcagcag agcgcaggtt actggcgtta aaatagtttt gattttcatg aaattacagt
    18421 ccttattacc agtagaaata ttgcatcagg aagatttggt cgctgggatc gttagaggca
    18481 tttttcatgt agaagcggct atcaaaagcc gtggcgaaac gtccgccgcc atattcgtac
    18541 cagataccaa actgcatgaa agaggtggtt tcatcgtcag cgtggtcagg gcgatgtttg
    18601 gcgtaggtgt aagcggtgga gagataaacg ccctttaacg cttcatacat ggcgctcacc
    18661 atcatcccgc tttcacttcc cggtacgtcc agcccgtcgg cacgtccggt atggtgccag
    18721 gcgcgagcgg caaagttagg ggtaaacagg cccgtgagat agagttgtcc tgtgccgtcg
    18781 gtaatttcca gaccattcat ccaggtgatg ccttcggcaa ggtcatactg gatgtaaccg
    18841 ttcatccatt gccggatagg tgtatttgtc gtcgtagcga tcgtacttgc caaaatgtag
    18901 ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg gtgacgctat aacgaaagtt
    18961 gccgcttaag ttctgtacct tcagcgcggt cataatgtcg cgggtattgg gaatgacgta
    19021 gcccaaatcg ggtgtgaagt cgccccacca ttgcaggtca tccagcgagg aatcctgacg
    19081 cagactgagc atgatttcgg tgccatcatt ggtacgataa ccgccataga aacggttaat
    19141 cccaccttcg aaaccacccc agctatggtc aggcacctag gcattaccgt cgtggtcggc
    19201 ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag gagccgtact tcatctccag
    19261 gccaccttca atataggtgc cgtcgctgta tttatgttta ttctcaccct ctaaatccat
    19321 atatccacca agccctaatt cgccgtaaaa acgaaatgaa ggagaagatg tcgaggtaat
    19381 tgcgggtgtt tttgtgtcgt catgttctgc cggacgtgct tcttctcctg ccgcagaaaa
    19441 ggcgtagcaa aacggcagag caagtaatgc tctgcatggt gcagtgatta atttcattgt
    19501 gttaatccat gagctaaaga aaaagtacag ccttttatcg ttgtaattat aaaaatataa
    19561 aggcagaaac tattttccct ctcctcatcc aggaagaggg aaaatgcctg ctcatttttt
    19621 attagtctgc ttaatcaaca ttgctggctg gcgtcgcttc aatatggcgc tcttcagccg
    19681 ttaatccctt tatatattta ctgtatttcc accacgcaaa gccgaggaag ataacaatac
    19741 cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc agtcgggaat gtcgaagcga
    19801 caaaccctac ggcaaatatc gcaatcagca ttgaaacaac gataataccg gtgcggcggg
    19861 agcccatacg gaaatcgcgt ggcaggtgat ctaatttggc gcgtaaattc aggtaagcca
    19921 gcatgatgaa taacggcgga agcatggacg ctgcggcggt catattaata atggtattca
    19981 tcagatcctg cacggtattg gaaccgagca tcgggataat catcagcggg atgacgatca
    20041 gaaactggat ccatgctgcg cgcgccggaa cgccgttttc gttaagttcg acggttttct
    20101 taccaaagat gccttccggg atttcggaga agaaaatttt caccggcgtt gcggtccaca
    20161 tcagcaggga accgaacatt gcggtaaagg acaccagacc gacaaagcga ttcatcaacg
    20221 cttccggcag accaaaatac gccgccatgc cgtggaatac ctgcaccgat ccgccggtaa
    20281 atttcaactc tttgctgctg acgaagacgt taatcagcac cgaggagacg gaatacagta
    20341 cgccgataaa aatcccggcg aggatgatca ctttaacgaa cgatttcgaa ccgcctttga
    20401 cgtcgttaac gtacaccgcg acggactccg cgccacctgc ggccataaag atccaggtgg
    20461 taacgccgag gaacgcccag ttgaagttcg ggatcatcgc gtcaacggtg atcggatcgg
    20521 caggctgtac gtcgccaacc agtgccgtac ctgccagtaa aatgtaggag agcgttaaca
    20581 gcagcatcag cgttgaagtg acggaggtaa ttggccccag cattttcgcc ccgttggtgg
    20641 aaacccaggt ggagaaggcg aacagcacca tactgataac ggtggtggca accggcgtca
    20701 taatatattc gtagccgagg aaggcatacg aagcataagc aataacgcgc ggcaacagcg
    20761 aggtgaaaaa gaacagattt acgaaccagt aagtgtaggc agtaataaat gcccaacgtc
    20821 cgcccagcga actttttacc cacgcgtaga caccggcttc tgagttttta tttaacgaaa
    20881 caaattctgc gatgatcaga cagaagggaa taaaataaaa aatcgtcgcg aggaaaaaca
    20941 tcggtgccga ggccagtcca agctcaatat tattattgat aacgttgtta aagctgtaaa
    21001 cggcggcaaa agtcagcgag agcaagccga atttgccgat tgtattacgt ttggtatcag
    21061 acataacaca tctccgaaaa tgtgtagggt agcggtggcg gcaaactcct tttgccgccg
    21121 tagttgttat ttgttatgga aataaccatc ttcgatggtg actttgagaa ccactttttt
    21181 gaccgcgtta ttgcagataa accgatacgc ttcatggata tcgcaaataa cgatttgccc
    21241 ttcgtggacc tctacggttt ctccgcagcc ttttaaatat tcacggtcag tttcatcacg
    21301 ataatattcc actacctgta atgtctcttt cggcgcatat tcaatttttt gctgcccttg
    21361 caggtagtaa tgcacttcaa aatagcgtcg atgcccggta aatagcgcat gggtcgcgga
    21421 gtcattttcc acacggtagg tcaatgaatc accgatggag tgggcgacgc caggctgaat
    21481 gttgtcgata ttttcaatcg cttcaacgca gcgttgccac ttcttgccag aggcgtaaat
    21541 ctggcggaac tgttctaagt tatcgatgat cctcattgct tattctcgct gtgcaaattc
    21601 gtggaaaaga accctgcgcc gaactcatac gacgccaggc tttgcgcggt agcttctccg
    21661 ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac ggaaccagac gcgccaggag
    21721 tccagcacct cgctgcccca ggagttggag ccgaggccaa gtagctggtg gtcgaggttc
    21781 agggtgatgt catcactgcg ctgcagctcg ttacagtgct gggcagcgtg gatgttttcc
    21841 tgggtatagt gccaggcgct gaagttaatt gggcgctgcg gaaccaccag cagaccgttg
    21901 tcgtggcggt tagtcagtgc cgtccagcgg acatgctgac ggttgccgtt gttctgcggg
    21961 aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc agatatcgat gatgttagcc
    22021 tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt aatacgccac ctgatcgtat
    22081 tcgccgttaa tccccatggt gaaaccgatg cacggaatga tgtgcggata gtcgccgtaa
    22141 cgctcgccgg aaagcgccac gttaacctgg ccatcggcag cgatgcgcca gatgtaggta
    22201 cagcgcatcc cgaagtcaaa caccggcggt gcaataactg tgcggctgat gatcaacact
    22261 tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt cctgcatgat ctgcaaatga
    22321 ttcggctgcc acaacccttc gtactcctgc ttgtggttgt cgatcatcgg cttgaagaag
    22381 ttgatctttg gctcgcgggt cagcagcgac tcgccattta cctgccagga tgtcggtttg
    22441 ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc gaacggtgca gctcaaacga
    22501 tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga aaggcactgg ctgcgcggtg
    22561 ttttccttca gcgggaactg ataagtggcg atagaatgcc cggcttcgct gtagcgggtg
    22621 cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc gggcgtccag ctgcggcagc
    22681 gtgatctgca agggggcttc gctgttcggc gcaacgtcgc gcagtttaat ctgctgcgtc
    22741 gcgagcgttt caccttcggc gcgcacctct gcgtgcaggg tgtagtcatc aagcgtggta
    22801 aaccacagtt tattttcgac tttcaactcg ccgcgagtca gcgcgtggat ttttaccggc
    22861 gcgataacct gtttgtactc tttcaggccc gatcccggcg tctgatcgga atagatcaaa
    22921 ccatcaagac agaagttata gttgttggga tagtcgccgt agtcgccgcc gaatttatac
    22981 cagacattgc cgtggtcgtc ctgtgcctgg atcccgtggt cgcaccactc ccagacataa
    23041 tgaccctgaa tgcaatcgtg cttatagaag acgttctggt actccgtcag cccgcccggt
    23101 ccgttcccca tcgcatgagc atattcacag atgatgcgcg gcttcggatg cgggtattca
    23161 ccaaactcat tcatcagcgg cacgcgggtg tacatggtgg aaataatatc gaccacttca
    23221 gcatcgcgat cttcttcgta atgcaccagt cgcgtgtcat ccagcgcttt cgccgcatgg
    23281 tacatcgcgc ggatgttaca gccatagccg gattcattgc ccagcgacca gatgatgatc
    23341 gacggatggt ttttctgtgc gtggatatgg cgaacaatgc gctcgacgta gactttttcc
    23401 cactgcggat cgtcggtaat acggctgata tcgccgacat tagcaaagcc gtgcgattcg
    23461 acgtcggttt ccgccatcac aaacaggccg tagatatcac acaattcgta aaaacgcgga
    23521 tcgttcgggt agtgagcggt acgcacggag ttgatattgt gctgcttcat caactggaga
    23581 tctttctcga cgcgatccat tccaacggcg cggcctttgc gatgatcgtt gtcgtgacgg
    23641 ttgacgccgt gcagcatcac ataacggtta ttgatccaga acagaccgtc gcgcactttg
    23701 atatcacgga agccaacgcg ttgtggcacc acttccagaa cgttgccgtt ggcgtctttc
    23761 agcgtcatga ccagatggta aagataaggg gattctgctg accattgctg cggctgttcg
    23821 acagtaaaag caaagctggc gctggtcagt ttttcaattg ccaaatgatc aatggcgctg
    23881 ctgtgcacca cgcgttcgcc atcaaacagg gtatattcca gcgtcgtgac gacaggggag
    23941 gcggcgagat tttccagcac cacttcgcag gaaagcgtgg catcgcaata ggcttcgtca
    24001 aagtcggtac gcacagtgaa atcgttaata tgcgttaggt gttttccgac cagataaaca
    24061 tcgcggaaga tccccgccga ccaccacata tcctggtctt ccacgtaggt agagtccgcc
    24121 cactgcatca cgcgcacaca caacaggttg tcgccggttt taaccatcgc gctgatgtca
    24181 aactctgcgg tcaggcgact gcccttgctg aaacccacat actgaccgtt aacgtagact
    24241 tcaaaatagg tttcgacgcc gtcaaattta atcagcgtct gtttaccctg ccagccgtcg
    24301 ctgagggtga aaatacgttg ataggcaccg gttgggttat cgctggggac aaacggcaca
    24361 tcgatgggga acggaaaacc ttcgtcggta tattgcagtt tgccgtgacc ttccatttgc
    24421 cacatggcgg ggacggtaat atgcccccag tcagccatta actcagaggt gaaggcttct
    24481 ggtacttgca gcggatggtc aaaaaagtgg aaattccact gaccgcttaa gggcagaaac
    24541 aggctgctgg tttcgcgggc aaaggtacgc gcttgcgcaa cagaatcata tgaaaaaaag
    24601 tacgcacgcg gcgcaagtcg gttttcgtgg gtgagctgaa tgttttccca gcgattcata
    24661 ggacctcccg gtagagagaa atcggaacca ttcatggtga tggtagtttg tgtaattgct
    24721 gggggggtta gtaaaatttt tactaaaagt agcaggtaaa tgcatttact ttaactggat
    24781 cacgaaaata aacggcagca ctgtccggca aaaatgattg ccggacagtg tgtaagggga
    24841 tttaacgggt cgtgccgcgc agttttaatt tgctgggaac gaagactagc agcggcagcg
    24901 cgcgaccatc gcgggctttt tcatacacca ggttaacgcc ctgacttccc atcatctcgg
    24961 aatggatgcg cacggtggag agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa
    25021 cgctgataag cgaaatatct tgtgggatgt tcaggccacg ttcatgaatt gcccgcagta
    25081 cgccgatagc aatggaatcg gaagcaacaa acagtgcctt cggatagtct tcccgcgcca
    25141 gcatttgttt tgccagttca taacccgacg aactggaaaa accgccgcgc cagatatcct
    25201 cttcgcgtac cacttgtttc agtcggccat attccgcaaa ggcgacctca cgaatatccg
    25261 ccttgccagg ctcatcttca ccgccaataa aaccaatacg attaacgccc tggttgatat
    25321 agaagtcgat gatttcttta ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc
    25381 tgccgggttc gtgaaagtcg ataaaacaga tattgtcggt caacgcgctg gcagcggcgc
    25441 gcagggcggg cgtgggtttg ccgacaatta aaataccggt gacgttttta atgtctggta
    25501 atccgttgtg ttcataacag ttggtcagtt cgatcgccag cttttcgcac tgagtttcaa
    25561 tgccgtggcg gatcgccaga tagtaaggat cgttgatctc cagctcctgc tggtagctgt
    25621 agatagccag aatatggtgt tggttgactg cgcctgtctg tagtttacgg gcgctactgg
    25681 tcttgtactc cagcttttcg gcgatctcga gaatgcgatg tttcgtctct tctttcacat
    25741 tcaatgtcgg atcgtcattt aagaccctgg atactgtcgc cagggatacg ccagcttcga
    25801 ttgcgatgtc ttttaatgtt gccatgttta ccttttttac tttccctgat gtgcgccccc
    25861 attgtagagg aagtgcttat cgggtcacct gttttcagta aataaagcgc gtttctttga
    25921 cgctgtaacg gtaacacagc ggcttaggaa tgtgatcacg cctgcattct ccatttttag
    25981 cctacaggat tgagaaaaac ttttgctata gtttagtaaa attttacccg aagggaggaa
    26041 gcaatggaaa ccgtggctta cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt
    26101 gtggaagtga aacgccatga aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg
    26161 aaaaaacact gcaaaccgtg accagcttgg tgccgtacta cagcgaagag gagctgatgg
    26221 ggaaaacggt ggtggtctta tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg
    26281 aatgcatgct gttgtgtgcg gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg
    26341 agcggatgat gcctgcgggc gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc
    26401 gccatccgat ttgatattac gcttcttcga cacttactcg catggccgcc agcgccttac
    26461 gcgccgcttt gatcaccagt tcacactgtt caatggtcag tgtcagtggc ggttcaatgc
    26521 ggatcgtttt ggcgttattg agcgttccgg ccaccagtac gcgctggcgg aacatctcgc
    26581 tggcaaagtt atagccgatt tcgttatcaa caaactcaat cgccatcaac atccctttac
    26641 cacgcgcttc ctgtaccaga tcgggatatt cccgcgccag ttgacggaaa ccgtccagca
    26701 acatatcgcc tttttgctca gcctgagccg gtaagttctg ctccagcaac acattgatgg
    26761 tcgccagcgc cgccgcacag gccagcgggt tgccgccaaa ggtggtggta tgcaggaatg
    26821 ggttgtcgaa cagaactgaa aacacctctt cagtggcgat ggtcgcgcca atcggcatca
    26881 cgccgccgcc gagggctttt gccagacaca gaatgtccgg ctgaacgttc tcatgttcgc
    26941 aggcaaacat cttgcccgtg cgccccatgc ccgtttgtac ttcatcgagg atcatcagtg
    27001 cgccgaactc atcgcacagc ttacgcacgg cggtgagata gcccggcggc ggcagaatta
    27061 cgccaccttc gccctgaatc ggttcgagga tcaccgcagc cacatcatca ccggtttttt
    27121 tgcactcatt aagagccgtg cgcatggctt cgatattgcc aaacggcaca tgacggaacc
    27181 ctggcagcag tggcataaac ggtttgcgga aggtcgattt cgccgtggcc gacagcgcgc
    27241 caagtgattt accgtggaac gcgccgctgg tggcaataaa agtaaatttg ccgcgcggcg
    27301 actgataagc cttcgccagc ttcagcgccg cttcaacgga ctcggtgccg ctattacaga
    27361 agaagctgta tttcagttta ccgggcgtta gcgcagcaag ggttttcgcc agcatagccc
    27421 gtagcggatc gagtagctcc tggctgtgca gtggttgttt cgcaagttga ttctgtacgg
    27481 cggaaaccac aactggatta cggtgcccca cgttgaaaat tccaaaacct cccaggcagt
    27541 cgataaactc ctgtccttgg gtgtcgacaa gcgtatttaa acctcccgct tgccactcta
    27601 cggctccgta atccccgccg gcggtaacag atttgcgata ctctaaaaac cccggattga
    27661 catgctcttt gaagtattca atcacctctc ggttaagtgc tttcatctcc tcatgatcca
    27721 gcgttcgctt ctcaatgaga ttcagggcgt gggcgctgca cgctaaagcc gatgcgctcg
    27781 aaggtaacct gttcaaaata tgctccggag gctcgcgtat cacatgatac cgatttaagt
    27841 attgcaggga ttgcgccact ccggctgcga tcgcgaaaat cgggataaac acaaatgcgg
    27901 ttatcatttg tgcaatattt aatgctatcc gttaacattt gttgttattt tgcacggttt
    27961 tgtcgggtat ggatttgatg attgctgcgc aaatgcacgt tttcagggca ataattgcct
    28021 gatttttggg caagataatt aacgttttcg tatagtaaca gagtgaaagt attttaaatc
    28081 aaagaattag atttgtgctt taatcgcaaa ttgcgatcta aatcaaatta atcggttaaa
    28141 gataaccgca gcggggccga cataaactct gacaagaagt taacaaccat ataacctgca
    28201 caggacgcga acatgtcttc tcatccgtat gtcacccagc aaaatacgcc gctggcggac
    28261 gataccactc tgatgtccac taccgatctg caaagctata tcactcatgc taatgacact
    28321 tttgtgcagg tgagcggctt taccttgcaa gagttacaag ggcagccgca caacatggtg
    28381 cgtcacccgg atatgccaaa agcggcgttt gcggatatgt ggttcaccct gaaaaaaggg
    28441 gagccctgga gcggcatcgt gaaaaatcgc cgcaaaaatg gcgaccatta ttgggtgcgg
    28501 gccaatgcgg taccgatggt gcgcgaggga aaaatcagtg gctatatgtc gattcgtacc
    28561 cgggcgacgg atgaagagat tgcggcggtg gagccgctgt acaaagcgtt gaactccgga
    28621 cgtaccagta agcgtattca taaaggcctg gtggtgcgta aaggctggct gggtaaactg
    28681 ccttcattac cgcttcgctg gcggacgcgt ggagtgatga ccctgatgtt tatcttgctg
    28741 gcggccatgc tttggtttgt tgctgccccg gtggtgacgt atttcctctg tgtgttagtg
    28801 gtattgttgg caagcgcttg ttttgaatgg cagattgtgc gcccgataga aaatgtcgcc
    28861 cgtcaggcac tgaaggtggc gaccggagag cgtaatagtg ttgagcacct gaatcgcagc
    28921 gatgagctgg ggctgacatt acgcgcggta gggcagcttg gcctgatgtg ccgttggcta
    28981 attaacgatg tctcaagcca ggtgtccagt gtcagaaatg gcagtgagac gctggcgaaa
    29041 ggcaccgatg aactgaacga acatacccag cagacagttg ataacgttca gcaaacggtg
    29101 gcgaccatga accaaatggc ggcgtcggtg aaacagaact ctgccacggc gtcggctgcc
    29161 gataaacttt ctatcaccgc cagtaatgcg gcagtgcagg gtggggaggc gatgaccacg
    29221 gtgatcaaga caatggacga tatcgccgac agtacccagc gcattggcac cattacttcg
    29281 ctgattaacg atattgcgtt tcagaccaat attctggccc tgaatgcggc ggtggaagcg
    29341 gcgcgtgccg gcgaacaggg caaaggtttt gcagtggtgg caggggaagt gcgtcattta
    29401 gccagccgca gcgctaatgc tgccaacgat attcgcaagc tgattgatgc cagtgctgat
    29461 aaggtgcaat ccggttcgca gcaggtacac gccgccggac ggacgatgga agatattgtg
    29521 gcacaggtga aaaacgtcac ccagttgatc gcccagatta gccattcaac gctggaacag
    29581 gccgatgggc tttccagcct gacccgtgca gtggatgagc ttaacctgat cacccagaaa
    29641 aatgccgagc tggtggaaga gagtgcgcag gtgtcggcga tggtgaaaca ccgcgccagc
    29701 cgactggaag acgcggtgac ggtgctgcat taatcggtat gccggatccg gcggttggag
    29761 cacaatgcct gatgcgatgc tgacgcatct tatcaggcct gcggggtgtg tagcgggtgt
    29821 aggccggata aggcgcgtca agcgtcgcat ccggcaattg caccgcgcca ctggcggatg
    29881 cggcgattaa tccagttgcg taatatcaaa agcggcgcgg tcgatcaccg caatgatctt
    29941 ttttatttgt gcatcactga tatcgctctg gttgacgcgt aaatccagca ccgctttaaa
    30001 attatccagc gcccgcttca tttgcgggtt ctggcgcagc gccgcgccaa cgcaacgcgc
    30061 tttgatgcgt tcttcaatca tctccacctg ttcgcggttt tcttccagcc actgcgcgcc
    30121 ttgttcggtc agcgcaatct gcttcttacc accttcctct tcgcggatgg taatcagcga
    30181 ctgctcctgc agaaaatcca gcgtcgggta gatgacgccc gggcttgggg tgtaattccc
    30241 ctgggttaga ttctcaatcg ctttaatcaa ttcgtaaccg tggctgtcat cgcgcgagag
    30301 aatatccaga atcaccagac gcaattcacc gtgaccaaag aaacgttgcc gacgaccgcc
    30361 gccgcgaccg tggcgaccac cgcaacattg accgtgttca tgctggtgac cgtgtccgca
    30421 gtgctcgtgt tctgacttct cacctttgca gcagccctca tggcgtggct ggccttcatg
    30481 tttacaacac ccttcgtgat gatggctcat atcagcctcc ttttgcttga ttagatatat
    30541 ctaatttata tctgatgtga gcattactgc aagtacaaaa atgattttta aacgtggaag
    30601 cacctcacat ctaggccgga taagacgttc acgccgcatc cggcagcatt gctatggcac
    30661 tgaatgcctt cacatacttc agacatatca gttctacaaa tcgcttgcat ttatcatgat
    30721 taacaatcat tatcatttgc gaattttatt tagatatatc tgattaccat cacgaaggcg
    30781 ataacaatga ataacacccc ccgctacccg cagcgcgttc gcaatgatct gcgcttccgt
    30841 gaactgactg tgctacgcgt tgagcgcatc agcgccggtt ttcagcgcat tgtccttggc
    30901 ggcgaggcgt tggacggttt tacatcgcgc ggctttgacg atcacagcaa actcttcttt
    30961 cctcaacctg acgctcactt tgtgccgcca acggtaacgg aagagggcat cgtctggccg
    31021 gaaggaccac gcccaccgtc gcgtgactat acgccactgt atgacgaact acgccatgaa
    31081 ctggcgatcg atttctttat tcacgacggt ggggtcgcca gcggctgggc gataaactta
    31141 cggtggcagg tccgcgcggt tcgctggtgg tgccggaaga ttacgcgtat cagctgtatg
    31201 tctgcgatga atccggaatg cccgcactgc gccgccgcct ggaaacgttg agcaaacttg
    31261 ccgttaaacc gcaagttagt gcgctggtta gcgtgcggga taacgcctgt caggattatc
    31321 tcgcgcacct tgatggtttt aatatcgaat ggctggcaca tgatgagcag gcggtagatg
    31381 cgcgtctggc gcagatgcaa atccctgcca gatgattact tcatctggat aaccggcgaa
    31441 ggtaaagtcg ttaagaattt aagccgccgc tttgaagcgg aacagtatga cccacagcgg
    31501 gtacgtgcag cggcttactg gcacgcaaag taactgatat ataaaaagaa aaaggctgac
    31561 gatttctcgt cagcctttgc tatatctggt ggcccctgct ggacttgaac cagcgaccaa
    31621 gcgattatga gtcgcctgct ctaaccactg agctaagggg ccatggtagc ggattataaa
    31681 gtaactccgt gtcgcaatcc agccattacc gcgcgcctgc tgtttttata aacaatgtat
    31741 tttcaatccg ttatactttt cctggtgatg ttagaaagga gtaaatatgg ttgaggatat
    31801 tttggctcca gggttacggg tcgtgttttg cggtatcaac cctgggcttt catccgccgg
    31861 gactggtttt ccctttgctc atccggcaaa tcgcttctgg aaggtgatat atcaggccgg
    31921 gtttaccgac cgtcagttga agccgcagga ggcacagcat ctgctggatt atcgttgtgg
    31981 cgtcaccaaa ctggtagacc gtccaacggt gcaagccaat gaagtttcaa agcaggagct
    32041 gcacgcaggt gggcgtaagc tgattgaaaa aattgaagat tatcagccgc aggcgttggc
    32101 gattctgggc aaacaagcat atgaacaggg attcagccag cgcggtgcac agtgggggaa
    32161 acaaacgctc accattggtt cgacgcagat ttgggtgctg ccaaatccca gcggtttaag
    32221 tcgcgtttca ctggagaaac tggttgaagc gtatcgcgag ctggaccagg cgctggtagt
    32281 gcgtgggcga taaaaaacgc caccga
//
LOCUS       DADLTF010000033        32160 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.033, whole genome shotgun sequence.
ACCESSION   DADLTF010000033 DADLTF010000000
VERSION     DADLTF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.033"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..407
                     /locus_tag="JN749_001423"
     CDS             <1..407
                     /locus_tag="JN749_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7367887.1"
                     /translation="PIFARVNKAGTPVAGLIIVGILMTIFQLSSISPNATKEFGLVSS
                     VSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTTIAFLYCIWAVVGSGAKEVMWS
                     FVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            511..2154
                     /gene="eptA"
                     /locus_tag="JN749_001424"
     CDS             511..2154
                     /gene="eptA"
                     /locus_tag="JN749_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HAY7367888.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            2151..2819
                     /gene="basR"
                     /locus_tag="JN749_001425"
     CDS             2151..2819
                     /gene="basR"
                     /locus_tag="JN749_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HAY7367889.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            2820..3920
                     /gene="pmrB"
                     /locus_tag="JN749_001426"
     CDS             2820..3920
                     /gene="pmrB"
                     /locus_tag="JN749_001426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HAY7367890.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(3896..3985)
                     /gene="pmrR"
                     /locus_tag="JN749_001427"
     CDS             complement(3896..3985)
                     /gene="pmrR"
                     /locus_tag="JN749_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HAY7367891.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(4097..5599)
                     /gene="proP"
                     /locus_tag="JN749_001428"
     CDS             complement(4097..5599)
                     /gene="proP"
                     /locus_tag="JN749_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HAY7367892.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(5863..6741)
                     /locus_tag="JN749_001429"
     CDS             complement(5863..6741)
                     /locus_tag="JN749_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367893.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNSGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(6738..8966)
                     /gene="crfC"
                     /locus_tag="JN749_001430"
     CDS             complement(6738..8966)
                     /gene="crfC"
                     /locus_tag="JN749_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HAY7367894.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            9368..9703
                     /locus_tag="JN749_001431"
     CDS             9368..9703
                     /locus_tag="JN749_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HAY7367895.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            9861..10304
                     /gene="yjdN"
                     /locus_tag="JN749_001432"
     CDS             9861..10304
                     /gene="yjdN"
                     /locus_tag="JN749_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HAY7367896.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            10437..11225
                     /gene="phnC"
                     /locus_tag="JN749_001433"
     CDS             10437..11225
                     /gene="phnC"
                     /locus_tag="JN749_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAY7367897.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            11250..12266
                     /gene="phnD"
                     /locus_tag="JN749_001434"
     CDS             11250..12266
                     /gene="phnD"
                     /locus_tag="JN749_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7367898.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSSGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            12372..13151
                     /gene="phnE"
                     /locus_tag="JN749_001435"
     CDS             12372..13151
                     /gene="phnE"
                     /locus_tag="JN749_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HAY7367899.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            13172..13897
                     /gene="phnF"
                     /locus_tag="JN749_001436"
     CDS             13172..13897
                     /gene="phnF"
                     /locus_tag="JN749_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HAY7367900.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            13898..14350
                     /gene="phnG"
                     /locus_tag="JN749_001437"
     CDS             13898..14350
                     /gene="phnG"
                     /locus_tag="JN749_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HAY7367901.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMIRGDNA"
     gene            14347..14931
                     /gene="phnH"
                     /locus_tag="JN749_001438"
     CDS             14347..14931
                     /gene="phnH"
                     /locus_tag="JN749_001438"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HAY7367902.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            14931..15995
                     /gene="phnI"
                     /locus_tag="JN749_001439"
     CDS             14931..15995
                     /gene="phnI"
                     /locus_tag="JN749_001439"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HAY7367903.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            15988..16833
                     /gene="phnJ"
                     /locus_tag="JN749_001440"
     CDS             15988..16833
                     /gene="phnJ"
                     /locus_tag="JN749_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HAY7367904.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            16830..17588
                     /gene="phnK"
                     /locus_tag="JN749_001441"
     CDS             16830..17588
                     /gene="phnK"
                     /locus_tag="JN749_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HAY7367905.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            17699..18379
                     /gene="phnL"
                     /locus_tag="JN749_001442"
     CDS             17699..18379
                     /gene="phnL"
                     /locus_tag="JN749_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HAY7367906.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            18376..19512
                     /gene="phnM"
                     /locus_tag="JN749_001443"
     CDS             18376..19512
                     /gene="phnM"
                     /locus_tag="JN749_001443"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HAY7367907.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPTSLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNHIHIDHVWRQGKRVF"
     gene            19512..20069
                     /gene="phnN"
                     /locus_tag="JN749_001444"
     CDS             19512..20069
                     /gene="phnN"
                     /locus_tag="JN749_001444"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HAY7367908.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLLVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            20056..20490
                     /gene="phnO"
                     /locus_tag="JN749_001445"
     CDS             20056..20490
                     /gene="phnO"
                     /locus_tag="JN749_001445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HAY7367909.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            20492..21250
                     /gene="phnP"
                     /locus_tag="JN749_001446"
     CDS             20492..21250
                     /gene="phnP"
                     /locus_tag="JN749_001446"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HAY7367910.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRAQRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHAQGLFPLRWGVGDPIPIYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLIENALPSGFEAGFDGMEIGVA"
     gene            complement(21397..21726)
                     /locus_tag="JN749_001447"
     CDS             complement(21397..21726)
                     /locus_tag="JN749_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367911.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(21795..22427)
                     /locus_tag="JN749_001448"
     CDS             complement(21795..22427)
                     /locus_tag="JN749_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7367912.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRAAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGCCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(22508..24723)
                     /locus_tag="JN749_001449"
                     /pseudo
     CDS             complement(22508..24723)
                     /locus_tag="JN749_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
     gene            24837..26357
                     /locus_tag="JN749_001450"
     CDS             24837..26357
                     /locus_tag="JN749_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7367913.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            26354..27328
                     /locus_tag="JN749_001451"
     CDS             26354..27328
                     /locus_tag="JN749_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7367914.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            27359..28300
                     /locus_tag="JN749_001452"
     CDS             27359..28300
                     /locus_tag="JN749_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7367915.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            28355..29038
                     /locus_tag="JN749_001453"
     CDS             28355..29038
                     /locus_tag="JN749_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HAY7367916.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQRLTLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            29053..29913
                     /locus_tag="JN749_001454"
     CDS             29053..29913
                     /locus_tag="JN749_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HAY7367917.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            29910..30911
                     /locus_tag="JN749_001455"
     CDS             29910..30911
                     /locus_tag="JN749_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HAY7367918.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(30908..31614)
                     /locus_tag="JN749_001456"
                     /pseudo
     CDS             complement(30908..31614)
                     /locus_tag="JN749_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            31905..>32160
                     /locus_tag="JN749_001457"
     CDS             31905..>32160
                     /locus_tag="JN749_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367919.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYY"
BASE COUNT         7429 a         9092 c         8647 g         6992 t
ORIGIN      
        1 caccgatttt tgcccgtgta aataaagcgg gtacgccagt ggcggggctg attatcgtcg
       61 gtattttgat gaccatcttc cagctcagca gcatttcacc aaacgcgacc aaagagttcg
      121 gtctggtttc ttccgtctcg gtcatcttta cactggtgcc atatctttac acctgtgcgg
      181 cgttactgct gctcggccac ggtcactttg gtaaagcacg cccggcatat ctggctgtta
      241 ctaccattgc cttcctctac tgcatttggg ccgtggtggg gtccggggcg aaagaggtta
      301 tgtggtcatt tgtcaccctg atggtcatca ccgccatgta tgcgctgaat tacaaccggc
      361 tacataaaaa cccgtatccc ttagatgcac caataagcaa agattaattc cccttaatcc
      421 agcaaacata aaagccaacc ttaagaactt aaggttggct taattttgct ttgcgagcat
      481 atgcgcactt tgttcgatgg aaacaccgtg atgttgaagc gcctactaaa aagaccctct
      541 ttgaatttac tcgcctggct attgttggcc gctttttata tctctatctg cctgaatatt
      601 gcctttttta aacaggtgtt gcaggcgctg ccgctggatt cgctgcataa cgtactggtt
      661 ttcttgtcga tgccggtcgt cgctttcagc gtgattaata ttgtcctgac actaagctct
      721 ttcttatggc ttaatcgaac actggcctgc ctgtttattc tggttggcgc ggctgcacaa
      781 tatttcataa tgacttacgg catcgtcatc gaccgctcga tgattgccaa tattattgat
      841 accactccag cagaaagtta tgcgctgatg acaccgcaaa tgttattaac gctgggattc
      901 agcggcgtgc ttgctgcgct gattgcctgc tggctaaaaa tcaaacctgc cacctcgcgt
      961 ctgcgcagtg ttcttttccg tggagccaat attctggttt ctgtactgct gattttgctg
     1021 gtcgccgcac tgttttataa agactacgcc tcgttgttcc gcaataacaa agagctggtg
     1081 aaatccttaa gcccctctaa cagcattgtt gccagctggt catggtactc ccatcagcga
     1141 ctggcaaatc tgccgctggt gcgaattggt gaagacgcgc accgcaaccc gttaatgcag
     1201 aacgaaaaac gtaaaaattt gaccatcctg attgtcggcg aaacctcgcg ggcggagaac
     1261 ttctccctca acggctaccc gcgtgaaact aacccgcggc tggcgaaaga taacgtggtc
     1321 tatttcccta ataccgcatc ttgcggtacg gcaacggcag tttcagtacc gtgcatgttc
     1381 tcggatatgc cgcgtgagca ctacaaagaa gagctggcac agcaccagga aggcgtgctg
     1441 gatatcattc agcgagcggg catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa
     1501 ggtgcctgcg accgcgtgcc tcaccagaac gtcaccgcgc tgaatctgcc tgatcagtgc
     1561 atcaacggcg aatgctatga cgaagtgctg ttccacgggc tggaagagta catcaataac
     1621 ctgcagggtg atggcgtgat tgtcttacac accatcggca gccacggtcc gacctattac
     1681 aaccgctatc cgccgcagtt caggaaattt accccaacct gcgacaccaa tgagatccag
     1741 acctgtacca aagagcaact ggtgaacact tacgacaaca cgctggttta cgtcgactat
     1801 attgttgata aagcgattaa tctgcttaaa gaacatcagg ataaatttac caccagcctg
     1861 gtttatcttt ctgaccacgg tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg
     1921 ccttatgcca tcgccccgga tagccaaaaa caggtgccaa tgctgctgtg gctgtcggag
     1981 gattatcaaa agcggtatca ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa
     2041 cactattcac aagacaattt attctccacg ctattgggat taactggcgt tgagacgaag
     2101 tattaccagg ccgcggatga tattctgcaa acttgcagga gagtgagtaa atgaaaattc
     2161 tgattgttga agacgatacg ctgttattgc agggactgat tctggcggcg caaaccgaag
     2221 gctacgcgtg cgatggcgtg acaaccgcgc ggatggcgga acaaagcctt gaggacggtc
     2281 attacagcct ggtggtactg gatttagggt tacccgacga agatggactg cattttctcg
     2341 cccgtatccg gcagaaaaaa tacaccctgc cggtactgat cctcaccgct cgcgatacgc
     2401 tgaccgacaa aatcgccggg ctggatgtcg gtgccgatga ctatctggtg aagccttttg
     2461 cgctggaaga gttacacgcc cgtatccgcg ccctgctacg acgccataat aatcagggcg
     2521 aaagtgagct gattgttggc aatctgacgc tgaatatggg tcgccgtcag gtatggatgg
     2581 gcggtgaaga gttgattctg acgcccaaag aatatgctct gctgtcacgg ttaatgctca
     2641 aagcaggcag tccggtacat cgggaaattc tctacaacga catctataac tgggacaatg
     2701 aaccctcgac caacaccctg gaagtgcata tccacaatct gcgcgacaaa gtgggcaaag
     2761 cccgtatccg caccgtgcgc ggctttggct atatgctggt cgcgaatgag gaaaactaat
     2821 tgaatctgat gcgttttctg cgccgaccaa tatcgctgcg ccaacggctg atattgacca
     2881 tcggggccat tttgttggtg tttgagctga tcagcgtctt ctggctatgg catgaaagta
     2941 ccgagcagat tcaactgttt gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca
     3001 tcatgcgtga gatccgcgag gcggtcgcca gcctgattgt ccccggcgtc tttatggtca
     3061 gcctgacgct atttatctgc tatcaggcgg tacgccgcat cacccgcccg ctggcggagc
     3121 tgcaaaaaga gctggaagcg cgcaccgccg acaacttaac gcccattgcc attcacagcg
     3181 ccaccctcga aatcgaagcg gtggtttcgg cgttgaacga tctggtcagt cgcctgacca
     3241 gcacgctgga taacgaaagg ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac
     3301 tggcgggggt gcgtttgcat ctggaactgc tggcgaaaac gcatcacatt gatgtagcac
     3361 cgttagtggc acggcttgat cagatgatgg agagcgtctc ccagctgctg caactggcgc
     3421 gtgccggaca gtcattttct tccggtaatt atcaacatgt aaaactgctg gaagatgtca
     3481 ttctcccctc gtatgacgaa ctcagtacca tgctcgacca gcgacagcaa accctgctat
     3541 tgccagagag cgccgccgac atcaccgttc agggtgatgc aaccctgctg cggatgttat
     3601 tgcgaaacct ggtagaaaac gcccatcgtt acagcccgca aggcagcaac attatgatta
     3661 agctgcaaga agatggcgga gcggtcatgg cagttgaaga tgaaggacca ggtattgatg
     3721 agagtaaatg cggggagttg agtaaagcgt ttgtacgtat ggacagccgt tatggcggga
     3781 ttggtctggg gttaagtatt gtcagccgca ttacacagtt gcatcacggg cagtttttcc
     3841 tgcaaaaccg gcaagagact tccggcacgc gggcctgggt acggctgaag aaagatcaga
     3901 acgtggcaaa ccagatataa agaaagctgc tgaccaccag cacgctgaac acggtagtta
     3961 aactttcata aacacggttt ttcattgcac tctcctccgt taacctggag gagagtatgc
     4021 gcgtcagaga ttaagtgaac cttaagagtt caattggcct gataagacag cgtcacatca
     4081 ggccatccgt ttcagcttat tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt
     4141 tcgcctgcaa atcggcaatc tcgtggtcaa tattatcgat tttctgctcg atattatcgt
     4201 aatgctcgac gagaatttcc ttcgcttcct gtatatctga cgccgccggt gtcgcacctt
     4261 tcaacggacg atttgccgtc tctttcatgg ttacgccggt gattaaacca atcaccgcca
     4321 ctaccatcag gtaataggca ggcatcatca gattctgcga gctttcgacc agccaggccg
     4381 ccagtgttgg cgtcagaccg gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc
     4441 tgtaacggat atgcgtcggg aacatcgctg gcaaggtaga agccataacg cccataaagc
     4501 agttaaggat caccgccagc atcagtaacc cggcaaaaat caggccgatg acgttactgt
     4561 taatcagaat aaacgccggg atcgccaaca caaacagtgc aacactacca agtagcacaa
     4621 acggacgacg gccaaaacgg tcactcagca agcccatcac cggctggaca aacagcatac
     4681 cgatcataat ggcgataata atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg
     4741 acaaataact cggcatatag gtcagcaaca tgtagtaagt cacgttggtg gcaattacca
     4801 gaccaataca tgtcaacagg ctgcgccagt atttagtggc aatctcttta aacgagactt
     4861 tcgggccatc ctgcaaacct tcgcggtcgc cctgttccag tttatcaaca tgctgctgga
     4921 acgccggagt ttcttccagc gcatggcgca ggtaaagccc gataatccct aacggcagag
     4981 caataaagaa cggaatacgc cagccccagt cgaggaagtt cgcttcgccg acaatggtcg
     5041 aaattaacac caccacgccc gcacccagca caaacccggc aatagaaccg aagtccagcc
     5101 agctgcccat aaagccacgt ttacggtcgg gggagtattc cgcaacaaat atcgacgccc
     5161 cggtatattc accaccgacc gagaaacctt gtgccatctt acagatcaac agcagaatcg
     5221 gtgcccaaat accaatcgtg tcgtaggacg gtattaagcc aatacagaac gtactgatcg
     5281 acataatcac aatagtgata gcgaggatct tctggcgacc atatttatcg cccaacatac
     5341 caaagaagag tccgccaagc ggtcgaatca gaaagggaac ggagaaagtg gcaagtgcag
     5401 caaccatctg cacgctgggg tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa
     5461 caaaaccata aacaccaaaa tcaaaccatt ccattgcatt acccagtgat gctgcggtaa
     5521 tggctttacg cagtttaccg tcatcaataa tggtgacatc acgaagggta atcggtttta
     5581 cttttttcct tttcagcata gctttcctcg cagagttgac ccatttccgc aaacgccaga
     5641 aaaatcagcg cgacggccgc aaaaggggag cgggaattgc aggatgcaat ctctgtaaca
     5701 agcgtagcgg gtttgcttac accctccggt taaggaatgt acaatcaaac cagttgacgc
     5761 ctgaattggg cagttaatga cctttttacc ctaccagtgt ttaaatttgt gatcaacttc
     5821 acacatggat ttcgtttagg actcattgat gtaactgatt atttacgcca aatttacaga
     5881 gaagatttcc tcgaccattg cccccaccaa acgctgcgct gaacagagtc gtggcatccc
     5941 cagcgcgaca gtttgccagc gttgggtcac tgaccagcaa acgggttgtt tatctgccgt
     6001 ggtccacttt cccagccaac ttaaaaaatc agcatgatcc ccgcccgtcg gaacggtcga
     6061 agaggcaaac cactgatggt aatagtgacg cgtggtcggt gccgcattga tactctctgc
     6121 cagatgttgt gtcgctttct cgcgcaaatc acccagcaac aacaccaccg cttgcgggct
     6181 tcccagacgg caggcatcgc caaatgcacc ccagcgcaac tcctccagaa cgataaaaca
     6241 gcgcccaggc aacgaccagg cgttatacgc cccagcacgc cagcgggtaa aaatttgctc
     6301 gctctgatca cctgcctgca ccagtaggcc tcgttgcgtt aacgcctttt ccttccagcg
     6361 acaacgggct gaaaaccact catacaaggc ggcaatttgc tgggggagct ccgggaaagc
     6421 agagtgctgg cggtgatcca ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag
     6481 cgccagatga ccgggatcca gcatcgacgc cagtttttcc accattgtca gtcgcagact
     6541 aagcggcgca tttaacatgc cgctgttcgt ccatgtctga agctgcacac gcgaaagaag
     6601 ttcctgcatc agtcgttcac gaaactgctg ctgatgggct tgaggatggc gggcatcatc
     6661 gccctgcgcc agatccacta aaaagcgcgg atgaaccgac tccagcacgc gaccggggcc
     6721 gtccagtaac gtcttggtca atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt
     6781 aacagctgag cgtcggtgta aatccagctg tgagtcatca cagtctgctg caattgctgc
     6841 tgcatcagcc tgaccaccga ttcattttgt tgacgcagag ccagactttc gcgtaaacgc
     6901 gtctgtaatt ctgtcaaaca tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta
     6961 acagattcat cgacctgctc tgccagaact ttagccattt gttggcaata gagatcgact
     7021 tctgcgctta atgctcgttg caacacactg taatcaaccg tttctgtcgg ggatttctca
     7081 tttccccgtc cccagtcggg ctgattcaac cagcgcgaaa aagtctcacg cacaacgcct
     7141 aaacgcgtgc tctgctcgtc cgttgcttcc tggcgcgaaa tgacttcact gaacagctgg
     7201 cgagtgttga agtggggaac tacgccgtga aaaacaggaa aatgaaaccc aggacgaaac
     7261 cctgactcgc tcaattccat tttgacttgt tgctcaatgg ggcgaataac atcggttaac
     7321 acccggcaaa gggtggattc cagctcggca aaacgcagcg taaagtcgcg actgatggtg
     7381 ttctgcgccg tctgtaacag tgtctcacag cgggtacgaa tctcgcttaa cggctccgaa
     7441 tcatcctgaa acaaggcggc taactgcgca ttcaccgcat cttgctgttg acgcagaaag
     7501 tggttggcgg aggtcagggc cagctcgatt tcatgtttaa tctcgccgct cacctgcgcc
     7561 tgattgagtt gcaatagctg caaactttct tcgacctgat ggatattttg ccgcaattgt
     7621 tcacaagcga cgtttaaccc gtgcgcacga aaatccaggt attcccgcgc ctgctgcgcg
     7681 taattcaaca gtttatgcgc agcagatcgc agagcataca atgaggcgtt agcgtaagcg
     7741 gcatgaagca acgcctgaat tggctgggcg aacagcgaat cttcccacaa ctgatcggca
     7801 gcatgacgaa tatgttcgag gtccgccaga tcggcatgac gccagcgcct gccgagcgcg
     7861 gcatgggcaa aatcttccac ccagcgttgt tgctctggcg ctggtaactt accgttgttg
     7921 gctaactcat agcgcgcccg attcgccagg tagccccaca tcgacgacac cgggaatatc
     7981 tgctgtggcg taatacagcc tttcatcagc gtcccggaaa tcagtgcccg cacctggtcg
     8041 gcgtcgtcac tgttacgatc ctgttgatcg aacttattga ccagcacata cagcggcacc
     8101 gattgcccca ccgccaaaat cgcctcacgg acctcttcat cggagatcga tttcagttgc
     8161 gtataatcca gcaccgccag taccgccgag gcgcgtgcca gctgctggtt aagcattttt
     8221 tgcagatgcg gttgcccggc ttcatttggc ccgggggtat ccagtaacgt caactgaccg
     8281 ggataacttt ccagccccgc cagatggaca aactccactt caatcacggg aatatgctca
     8341 atggcggcgt aagcagaaaa aggaaaatcg acgtccagcg ccttcgccag tcgcactaaa
     8401 tcattcaaac ttttcagaca atgaaaaata ggctgggcac ccagataata tttttcgaaa
     8461 gcgacgccat tttcgatccg ctgcataagc gcacgcatat ctttatctat ttccagcaca
     8521 tcggtcagat gcttaatatc gcaatcacgc aggcgctgtt gtaattgttg aattaaacaa
     8581 tcgattggcg cgacatgtga aaaatgcagt accggttcct tttgcccggg cgtatggcga
     8641 ataagcgtcg gcagcgcagt cattgggcga ttacgattag gcagaacctc cgtaccaaca
     8701 atggcattaa tggtggttga tttccctgct ttcatggtac cgacaattgc cagcaccatt
     8761 tccagtcggg aaattttacg caactcatta ttcagcatcg cgtgacgttc ggcgatatta
     8821 ggctgactcc agggtaaagc cagttgtggc gcgtcgtctc cgggtacaga gagaggcatt
     8881 ttttccagta actgcaactg ttggcgagaa agctgtaaca ggcgttcagc ctcctgactt
     8941 aactcataca gggtctgtgt gtacatagaa aattcttcct taaagcaaat tttgttactt
     9001 ttatttagcc agattgtttt tgagttctgt tttcggcttt tataattact gcaagaaata
     9061 attttatatt tagtgtgttg ttttttatca gaataaataa cgtcttctga tgcgtttaaa
     9121 acgtcagaaa gataaaaata tcatgtaaat aaaaaaaaga acaagtagag cattaacatt
     9181 atcttaaata ataaatagag gcaaaaagat tattttcttt ttgcgtttcc tttcaaatga
     9241 aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac tcagggattt tgtgtataat
     9301 tgcggccttt ttcggcaatc tgccgttttt tggcgctttt gccctgctga cttttgagga
     9361 aatccacatg tcattaccac actgcccaaa atgcaactcc gaatacactt acgaagataa
     9421 cggcatgtac atctgcccgg aatgtgccta cgaatggaac gacgcagaac ctgcacagga
     9481 aagcgacgag ctgatcgtta aagatgctaa cggcaatctg ctggctgacg gcgacagcgt
     9541 taccatcatt aaagatctga aggtgaaagg tagctcttcg atgctgaaaa ttggcaccaa
     9601 agtgaaaaac atccgcctgg ttgaaggcga ccataacatc gattgcaaaa tcgacggttt
     9661 tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac tgattgtatt gtgatcggta
     9721 agccgaataa ggcgctcgcg ccgcatccgg caacgatgcc gactgcctga tgcgacgctt
     9781 gcgcgtctta tcaggcagcc tctcatctgt ataaatttcg aactacactt aactggcttc
     9841 tcttaactga ggtcaccatc atgccgttaa gtccctacct ctcttttgcc ggtaactgtt
     9901 ccgacgcgat tgcctattat caacgtacgt tgggcgcgga actgctctat aaaatcagct
     9961 tcggcgaaat gccgaaatca gcgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat
    10021 ttcccgatac cgccatcgct catgccaacg tgcgcattgc cggaagcgac atcatgatga
    10081 gcgatgctat accgtcagga aaagccagct actccggctt tacgctggtg ctcgattcgc
    10141 aacaggtcga agaaggaaaa cgctggtttg ataaccttgc cgctaacgga aaaatcgaaa
    10201 tggcctggca ggaaactttc tgggcgcatg gctttggcaa agtcaccgat aaatttggcg
    10261 taccgtggat gattaatgtc gtcaaacaac aaccaacgca ataacccgcc gggaggcccg
    10321 ccctcccgca ctgtcatcga attcccgtta actcttcatc tgttagtcac ttttaattaa
    10381 ccaaatcgtc acaataatcc gccacgatgg agccactttt ttagggaggc tgcatcatgc
    10441 aaacgattat ccgtgtcgag aagctcgcca aaaccttcaa tcagcatcaa gcgctgcatg
    10501 cggttgatct gaacattcat cacagtgaaa tggtggctct gcttgggccg tcgggttccg
    10561 gcaaatccac ccttttacgt catttaagcg gtttgattac cggcgataaa tccgccggta
    10621 gccatatcga gctactgggc cgcactgtcc agcgcgaagg ccgcctggcc cgcgatatcc
    10681 gcaaaagccg cgccaacacc ggctacatct ttcaacaatt caacctggtg aaccgcctga
    10741 gcgtactgga gaacgtgctg attggcgcgc tcggcagcac gccgttctgg cgcacctgtt
    10801 ttagctggtt caccggcgag cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca
    10861 tggtgcattt tgcccatcag cgcgtttcca ccctctccgg cggccagcag caacgtgtgg
    10921 cgattgcccg tgcgctgatg cagcaggcga aagtgattct ggccgatgaa cccatcgcct
    10981 cgctggaccc ggaatcagcg cgcatcgtga tggacaccct gcgcgacatc aaccagaacg
    11041 acggcatcac cgtggtcgtc acgctgcatc aggtggatta cgccctgcgc tactgcgaac
    11101 gcatcgtcgc cctgcgtcag gggcacgtct tctacgacgg cagcagccaa ctgtttgata
    11161 acgaacgttt tgaccatctc taccgcagca ttaaccgcat cgaagagaac gcgaaagctg
    11221 cctgatatcc ccatcattga ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct
    11281 tcaccagcat gttcagcctc agcaccctgt taaccccggc acacgccgaa gagcaggaaa
    11341 aggcgctgaa tttcggcatt atttcaacgg aatcacagca aaacctgaaa ccgcaatgga
    11401 cgccgttttt acaggatatg gagaagaaac tgggcgtgaa ggtcaacgcc ttctttgccc
    11461 cggactacgc gggcattatc caggggatgc gcttcaataa agtggatatt gcctggtacg
    11521 gcaacctgtc ggcgatggaa gcggtggatc gcgccaatgg ccaggtcttc gcccagacgg
    11581 tcgcggcgga tggatcgtcg ggttactgga gcgtgttgat cgtcaacaaa gacagcccga
    11641 tcaacaacct gaacgatctg ctggcgaagc ggaaagatct caccttcggc aatggcgatc
    11701 ctaactccac ctctggcttc ctcgtccccg gttactacgt cttcgctaaa aacaatatct
    11761 ccgccagcga cttcaagcgc accgtcaacg ccgggcatga aaccaacgcg ctggccgtcg
    11821 ccaacaagca ggtggatgtt gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct
    11881 ccgcgccgga gaagcttaaa gagctgaagg tgatctggaa atcgccgctg atcctaggcg
    11941 atccgatcgt ctggcgcaag aatctgtccg aaactaccaa agacaagatc tacgacttct
    12001 ttatgaacta cggaaaaacg ccggaagaga aagcggtgct ggaacgccta ggctgggcgc
    12061 cgttccgcgc ttccagcgac ctgcaactgg tgccgattcg ccagctcgca ctgtttaaag
    12121 agatacaaag cgtgaaaggc aataaagggc tgaatgagca ggacaagctg gcgaaaacca
    12181 ccgagattca ggcgcagctg gatgacctgg accgcctgaa caacgcgtta agcgcgatga
    12241 gttcggtgag taaagcggtg cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat
    12301 ccgacaatgt gcaggcgttg atgccggatg cggtgcaagc accttatccg gcctacagac
    12361 cggagccaaa catgcaaacc atcaccatcg ccccacccaa gcgtagctgg ttctcgcttc
    12421 tgagctgggc cgttgttctc gccgtgctgg tcgtctcgtg gcagggcgcg gaaatggccc
    12481 cgcttacgct gattaaagac ggcggcaaca tggcaacctt cgctgccgac ttcttcccac
    12541 ccgatttcag ccagtggcag gattacctca ccgaaatggc cgtcacgctg caaatcgccg
    12601 tctggggcac cgcgctggca gtggttctct ccatcccctt cggtctgatg agcgccgaaa
    12661 acttggtgcc gtggtgggtt taccagcccg ttcgccgcct gatggacgcc tgccgcgcca
    12721 ttaacgaaat ggtcttcgcc atgctgttcg tggtcgccgt cggcctcggc ccgttcgctg
    12781 gcgtgctggc gctgtttatc cacaccaccg gcgtgctctc caagctgctt tccgaagcgg
    12841 aggaagcgat cgaacccggc ccggtggaag gcattcgcgc caccggcgcc aacaaactcg
    12901 aagagatcct ttacggcgtg ctgccgcagg tgatgccgct gctgatctcc tactccctct
    12961 atcgcttcga atccaacgtc cgctcggcga ccgtcgtcgg catggtcggc gcgggcggga
    13021 tcggcgtcac cctgtgggaa gcgattcgcg gtttccagtt ccagcaaacc tgcgccctga
    13081 tggtgcttat catcgtcacg gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc
    13141 actttatctg ataagcgagg cattgatatc tatgcacttg tctacacatc cgaccagcta
    13201 cccaacacgc tatcaggaga tagccgcaaa acttgagcag gagcttcgtc aacactaccg
    13261 ctgcggcgac tatcttcccg ccgagcagca actggcggcg cgctttgagg tgaaccgcca
    13321 caccctgcgc cgcgccatcg accaactggt ggaaaaaggc tgggtacagc gccgtcaggg
    13381 cgtcggcgtg ctggtgctga tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt
    13441 tagccagaac ctgctggacc agggcagcca tcccaccagc gaaaaactgc tgtcggtatt
    13501 gcgccccgcg tccggtcacg ttgctgacgc gctggggatt accgagggcg agaacgttat
    13561 ccatttgcgc accctgcgcc gggtcaacgg cgtcgcgctc tgtttaatcg accactactt
    13621 tgcggacctc accctttggc cgacgctgca acgcttcgac agcggttcgc tgcacgattt
    13681 tctgcgcgag caaaccggaa ttgcgctgcg ccgcagccag acgcggatca gcgcccgccg
    13741 cgcccaggcc aaagagtgcc agcgccttga aatcccgaat atgtcgccgc tgctgtgcgt
    13801 gcgcaccctt aaccaccgtg acggtgaaag cagcccggcg gagtactccg tcagcctgac
    13861 gcgcgccgac atgattgaat tcactatgga gcactgaatg cacgcagata ccgcgacccg
    13921 ccagcactgg atgtccgtac tggcgcacag ccaaccggct gaactggcag cccgcctgaa
    13981 cgcgctgaac atcaccgccg actatgaagt gatccgcacc gccgaaaccg gcctggtaca
    14041 gattcaggcg cgaatgggcg gcaccggcga acgctttttt gccggcgacg ccacgctgac
    14101 ccgcgccgcc gtacgcctga ctgacggcac cctcggctac agctgggtgc tggggcgtga
    14161 taaacagcac accgaacgct gcgcgctgat tgatgcgctg atgcagcaat ctcgccactt
    14221 tcaaaactta tcagaaaccc ttattgcccc gctggacgct gaccgtatgg cacgcattgc
    14281 cgcacgccag gccgaagtga acgccagccg ggtcgacttc tttacgatga ttcgcggaga
    14341 caacgcatga ccctggaaac cgcttttatg cttcccgtgc aggatgccca gcacagtttt
    14401 cgtcgcctgt taaaggccat aagcgagccg ggcgtgattg tcgccctgca tcagctcaag
    14461 cgcggctggc agccgctgaa tatcgccacc accagcgtgc tgctgacgct ggctgataac
    14521 gacacgccgg tgtggcttgc cgcgccattg agtaacgata tcgtcaatca gagcctgcgt
    14581 tttcatacca acgcgccgct ggtcagccag ccggaacagg cgaccttcgc ggtgacggat
    14641 gacggaattt ccagcaaaca gctcaacgcc ctttccaccg gcaccgccgt tgcgccggaa
    14701 gcgggcgcga cgctgatttt acaggtcgcc agcctgagcg gcgggcgcat gttgcgtctc
    14761 accggcgcgg gtattgccga agaacgaatg atcgctccgc agctgccgga gtgcattctg
    14821 cacgaactca ccgagcgccc gcacccgttc ccgctcggca tcgacctgat cctgacctgc
    14881 ggcgaacgcc tgctggctat tccgcgaacc actcatgtgg aggtgtgctg atgtacgttg
    14941 ccgtgaaagg gggcgaaaag gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc
    15001 ggggcgatac cgatttgccc gaactgagcg tcgcccaaat tgaacagcag cttaatctcg
    15061 cggtggatcg cgtgatgacc gaaggcggcg ttgccgaccg cgaactggcg gcgctggcgc
    15121 tgaaacaggc cagcggcgat aacgttgaag caattttcct gctgcgcgcc taccgcacca
    15181 cgctggcgaa actggcggta agcgagccgc tcgacaccac cgagatgcgt ctcgaacggc
    15241 gtatttcggc ggtttataaa gacattcccg gcggccagct gcttggccca acctacgact
    15301 acacccatcg cctgctcgat ttcaccctgc tggcaaacgg tgaagcgccg acgctgacca
    15361 ccgccgacag cgaacaacag ccgtcgccgc acgttttcag cctgctggcg cgtcaggggc
    15421 tggcgaagtt tgaagaagat agcggcgcac agccggatga catcacccgc acgccgccgg
    15481 tttacccctg ctcacgctcc tcccgcctgc aacaattaat gcgcggggac gaaggctatt
    15541 tgctggcgct ggcctactcc acccagcgtg gttacggacg caatcacccg ttcgcgggcg
    15601 agatccgcag tggttacatc gacgtgtcga ttgtgccgga agagctggga tttgcggtaa
    15661 acgtcggcga actgctgatg accgagtgtg aaatggtcaa cggttttatc gacccgccgg
    15721 atgagccgcc gcacttcacg cgcggctacg ggctggtatt cggcatgagc gagcgcaaag
    15781 cgatggcaat ggcgctggtt gaccgtgcgt tgcaagcccc ggaatacggc gagcacgcga
    15841 caggcccggc gcaggatgaa gagttcgtgc tggcgcatgc cgacaacgtc gaagccgcag
    15901 gctttgtctc gcacctcaaa ctcccccact acgtcgattt ccaagccgaa ctggagctac
    15961 tcaaacgtct gcaacaggag cagaaccatg gctaatctga gcggctacaa ctttgcctac
    16021 ctcgacgagc agaccaaacg catgatccgc cgcgccatct taaaagcggt ggctatcccc
    16081 ggttatcagg tgccgtttgg cgggcgcgag atgccgatgc cctacggctg gggaaccggc
    16141 ggcattcagc tcaccgccag cgtgattggc gaaagcgacg tgttgaaggt gattgaccag
    16201 ggcgcagacg acaccactaa cgccgtgtcg attcgcaact tcttcaagcg cgtgaccggg
    16261 gtgaacacca ctgaacgtac ggacgatgcg acgcttatcc agacgcgcca tcgcatcccc
    16321 gaaacgccgc tgaccgaaga tcagatagtt attttccagg tgccgatccc cgaaccgctg
    16381 cgctttatcg agccgcgcga aacggaaacc cgcactatgc acgcgctgga ggagtacggc
    16441 gtgatgcagg tgaaactgta tgaagatatc gcccgcttcg gtcatatcgc caccacctat
    16501 gcttatccgg tgaaggtgaa cgggcgctac gtgatggacc cgtcgccgat cccgaaattc
    16561 gataacccga aaatggacat gatgcccgcc ctgcagctgt tcggcgcagg gcgcgagaag
    16621 cgcatctatg cggtgccgcc gtttacccgc gtggaaagtc tcgatttcga cgatcacccg
    16681 ttcaccgttc agcagtggga tgagccatgc gccatctgcg gatcgaccca cagctatctc
    16741 gacgaagtgg tgctggatga cgccggaaac cgcatgtttg tctgctccga taccgattat
    16801 tgccgccaac agagcgaggc aaaaaaccaa tgaatcaacc gttactttcc gtcaataacc
    16861 tgacccacct ttacgcgccg ggcaaaggct ttagcgatgt ctcttttgat ttatggccgg
    16921 gggaagtgct gggcatcgtc ggggaatccg gctccgggaa gaccacgctg ctgaaatcaa
    16981 tctccgcgcg cctgacgccg cagcaggggg agattcgcta cgagaatcgt tcgttgtatg
    17041 cgatgagcga ggccgaccgc cgtcgcctgc tgcgcaccga atggggcgtg gtgcatcagc
    17101 atccactcga cggcctgcgt cgccaggtct cggcaggcgg caatatcggc gagcggctga
    17161 tggcgaccgg ggcccgtcat tacggcgaca tccgcgccac cgcgcagaag tggctggaag
    17221 aggtggagat tcccgccaac cggatcgacg acctgccgac caccttttcc ggcggcatgc
    17281 agcaacgttt gcagattgcc cgcaacctgg tgacgcatcc gaagctggtg tttatggacg
    17341 agccaaccgg tgggctggat gtgtcggtgc aggcacgcct gctcgatcta ctgcgcggcc
    17401 tggtggtgga gctgaacctg gcggtggtga ttgtcaccca tgatttaggc gtcgcccgcc
    17461 tgctagcgga ccgtttgctg gtgatgaagc aggggcaagt agtggagagt gggttaaccg
    17521 accgcgtgct cgacgacccg caccatccgt atacccagct gctggtgtca tcggttttgc
    17581 agaattgagc cggtgccgga tgcggcgtaa acgccttatc tggcctacaa atgcgctccc
    17641 cgtaggtcgg ataagacgca tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat
    17701 gattaacgta caaaacgtca gtaaaacctt catcctgcac cagcaaaacg gcgtgcgcct
    17761 gcccgtcctc aaccgcgcct cgctcaccgt caacgcgggc gaatgcgtgg tgctccacgg
    17821 ccactccggc agcggcaaat caaccctgct gcgctcgctg tacgccaact atctgcccga
    17881 tgaagggcaa atccagatca aacacggtga cgagtgggta gacctggtca ccgcgccagc
    17941 gcgcaaagtg gtggagatcc gcaaaaccac cgtcggctgg gtgagccagt ttctgcgcgt
    18001 catcccgcgt atttcagcac tggaagtggt gatgcagccg ctgctcgata ccggcgtacc
    18061 gcgtgaagcc tgcgccgcta aagccgcgcg tctgctcacc cgcctgaacg tgccggaacg
    18121 cctgtggcac ctggcgccat caaccttctc cggcggcgaa cagcagcgcg tcaacatcgc
    18181 ccgcggcttt atcgtcgact acccgattct gctgctcgac gaacctaccg cctcgctgga
    18241 cgccaaaaac agcgccgcgg tggtggaact gatccgcgaa gccaaagctc gtggcgcagc
    18301 cattgtcggc atcttccatg acgaagccgt acgtaatgac gttgccgacc gcctgcaccc
    18361 aatggaagcc tcttcatgat tatcaataac gtcaaattgg tgctggaaaa cgaagtggta
    18421 cacggttcac tggaggtgca ggacggcgaa atccgcgcct ttgccgaaag ccagagccgc
    18481 ctgccggagg cgatggacgg cgaaggcggc tggctgctgc cggggctgat tgagctgcat
    18541 accgataatc tggataaatt cttcaccccg cgcccgaaag tcgactggcc cgcccattcg
    18601 gcgatgagca gccacgacgc gctgatggtg gcaagcggca tcaccaccgt actggacgcc
    18661 gtggcgattg gtgatgtgcg cgacggcggc gatcggttgg agaatctgga gaagatgatc
    18721 aacgccatcg aagagacgca gaaacgcggc gtcaaccgcg ccgagcaccg tctgctcctg
    18781 cgctgcgaac tgccgcatca caccacgctg ccgctgtttg aaaaactggt gcagcgcgag
    18841 ccggtgacgc tggtgtcgct gatggaccac tcgccgggcc agcgccagtt cgccaaccgc
    18901 gagaagtatc gcgaatatta tcagggcaaa tactcgctca ccgatgcgca gatgcagcag
    18961 tacgaagaag agcaactggc gcttgccgca cgctggtcgc agccgaatcg cgaatccatc
    19021 gccgccctgt gccgcgcgcg acaaattgcg ctcgctagcc atgatgacgc cacccacgcc
    19081 cacgttgccg aatcccacct gcttggcagc gtgatcgccg aatttcccac cacgtttgaa
    19141 gcggcggaag cctcgcgcaa gcatggcatg aacgtactga tgggcgcgcc gaatatcgtg
    19201 cgcagcggtt cgcactccgg caacgtggcg gccagtgaac tggcgcagct tggcctgctg
    19261 gatatcctct cttccgacta ctaccccacc agcctgctgg atgcggcatt tcgcgtcgcc
    19321 gatgaccaga gcaaccgctt tacgctgccg caggcggtga agttagtgac ccaaaatccg
    19381 gcgcaggcgc ttaatcttca ggatcgcggg gtgattggcg agggcaaacg cgcggacctg
    19441 gtgctggcac atcgcaagga caatcatatt catatcgacc acgtctggcg tcagggtaaa
    19501 agggtgttct gatgatggga aaactgattt ggttaatggg gccgtccggc tccgggaaag
    19561 acagcctgct ggcggaaatc cgcctgcggg aacaaactca gttactggtg gcgcatcgct
    19621 acattacgcg cgacgccagc gccggaagtg aaaaccatat cgccctgagc gagcaggagt
    19681 tttttacccg cgcggggcaa aatctgttgg ccttaagctg gcacgctaac agtctgtatt
    19741 atggcgtcgg cgttgagatt gatctctggc tgcacgccgg attcgacgtg ctggtcaacg
    19801 gctcacgcgc ccatctgccg caggcgctgg cgcgctatca atcggcgctg ctgcccattt
    19861 gcttactggt ttcgccggag atcctgcgcc agcgcctcga aaaccgtggt cgtgaaaatg
    19921 ccagtgaaat taacgcccga ctagcacgcg ctgcccgcta tactcctcag gattgtctta
    19981 cgctcaataa tgacggcagc ctgcgccagt cggtcgacaa gctgctgacg ctgattcatc
    20041 agaaggagaa acaccatgcc tgcttgtgag cttcgcccgg ctacgcagta cgacaccgac
    20101 gcagtttacg cgctgatttg tgagctaaaa caggcggagt ttgaccacca tgcgtttcgc
    20161 gtgggcttta acgccaacct gcgcgaccca aacatgcgct accatctggc gctgctggat
    20221 ggcgaagttg tcagcatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg
    20281 atcggcgaaa ttcaggaact ggtggtgatg ccgcaggcgc gcggtctgaa cgtcggcagt
    20341 aaattactgg catgggcaga agaagaagcc cgccaggccg gggccgaaat gaccgcgctt
    20401 tcgaccaacg tgaaacgcca cgacgcgcac cgtttctatc tgcgagaagg ctacgagcag
    20461 agtcacttcc gcttcaccaa ggtgctgtaa catgagcctg accctcacgc tcaccggcac
    20521 tggcggcgca cagggcgttc cggcatgggg ctgcgagtgc gcggcctgcg accgagcgca
    20581 gcgctcaccg cagtatcgcc gccagccatg cagcggcgta gtgaagttta acgacgcaat
    20641 caccctgatc gacgccgggc tgcacgatct cgccgatcgc tggtcgcccg gatcgttcca
    20701 gcagtttttg ctgacgcatt atcatatgga tcacgcccag gggctgtttc cgctgcgctg
    20761 gggcgtgggc gatccgatcc cgatctacgg cccgccggat gaccagggtt gcgacgatct
    20821 gtttaaacat ccgggcctgc ttgatttcag ccacacagtg gaaccgtttg tggtgtttga
    20881 tttgcagggg ttgcaggtca cgccgctgcc gcttaaccac tcaaaactga ccttcggtta
    20941 tctgctggaa acggcacata gccgggtggc gtggctatct gacaccgctg gcctgccgga
    21001 aaaaacgctg aaatttttac gcaataatca gccacaggta atggtgattg attgcagcca
    21061 cccgccgcgc gcggatgcgc cgcgtaatca ctgtgattta aataccgtgc ttgcgctgaa
    21121 tcaggttatc cgctcgccac gggtgattct gacccatatc agccaccagt ttgatgcgtg
    21181 gctgatagaa aacgcactgc cgtcggggtt tgaggcgggg tttgatggga tggagattgg
    21241 ggtggcgtga tgagagggaa tgtgcgcgct ggtcccctca ccctaaccct ctccccagag
    21301 gggcgagggg agtgattgtg ctcgatattg aatattgcgc tggttttctc cctctcccca
    21361 ttggggtgag gggcgatgcc tgctccatac ccaacctcat cgcccatact catcttccat
    21421 tctccgctct tcatcttcta gctgccgacg ctcctgatca agctggcgct ggcgatcgtc
    21481 cagctgcctg cggcgatctt caaactggca gcggcggtcg tcatattgtc tgcgccgatc
    21541 gtcgctcact tcacgctgcc agccgtcgtc gcgcgaatct tcatagtctc gcccacggtc
    21601 agagttataa gcgtcattaa tcgcctgctg aatattacca atagtatcgt cgataatatc
    21661 ggcctgggcc ggaacggtgg acagcgtgag cagggtgaaa agaaaaagcg tggagtaagg
    21721 tttcataagc cagtctctgc gatggactgg cttaacgtta gttgagtgtg aatgaccgga
    21781 gtagcggagt aatctcaaat tcggtaaggc gcagactctt ccattagctg gttagctcgc
    21841 tgtttatttt ccgccaccaa tacaatgcca tgacattttt gatccggtga aatactccag
    21901 atacgctctt ccggaatgca gcagccatcg tagatcactt tcgtgccatc gtagggccaa
    21961 aatgcccctt gttcagtgtc taatacaaac acggaaatct gatcggactc gcgcgcgatc
    22021 ttctgccaga gcatcttccc gccatcatat tgatcaaaat cgctgattag cgtgataccg
    22081 cattgcagga ttagcgcttc atacagccga gttgccagac ccttatcgcg ctcacctgga
    22141 gagacacaaa taccttccac ctgtaccaga tccgagtctt cccaccagcg ttcaacggca
    22201 atgatatcgc tgccgaactc ttttttacgc tgcagatcga gagcggcaag tacccgatga
    22261 cgcggcacgc ctgcaaaaac ttcaatgtcc gcttcaatga tggccgctct tggcgtctgg
    22321 ttgtcataaa ttacctgtaa cagacgtcca gaaagctcga tctcttccac cacgcgaaaa
    22381 gaacgagcaa ccgtcatagg gctataggtg gttggcatca cgatcatatg taaatcactt
    22441 caattcagaa cagaattgct cacaatagca gcacgtttgc ttcgagcctc ttgataaatt
    22501 ctgaaaatca tccctcaatt tgccccagca cctgcgccag ctgcgcgcgg gtaaagggtt
    22561 tgcgcagcag cgccacgtcc ggcagcgcgg ggttatggct ggggcgtaaa tcctgaccgc
    22621 tgatgagtag cagcgtcagg tgcgggtaaa gtttgcgggc cgcattgacc acttctgcgc
    22681 cgctcatgcc gccagcaggt ccagcgcctg ttcgccgctg ctcgcttcca gtgtcaaata
    22741 gcccagcagg tgcaactgct cgcagatggt ctggcgcacc gcggcttcat cttccagcac
    22801 taacaccagt ttatcgccat taacagctgt ctgttctacc gccgctggcg ataggttttt
    22861 cgccgccgtc agcgcgcggg gaagctgtaa tctgaccgtt gtcccctgcc ccggcgcgct
    22921 ttcaatttcc acgcgcccgc cggactggcg cacgaaacca tagaccatcg acaagcccag
    22981 cccgctgccg ctccccgtct gtttggtggt aaagaacggc tcgaacacct ggcttttcac
    23041 cgcctgcgac ataccgctgc catgatcgat gacctccagt gccaccatgt cctgacgctg
    23101 cccactgctg cgggtaacac gctgattcca ggtacgaatt ttgataattc ccgtctgccc
    23161 ttccatcgcg tcgcgggcgt tcatcaccag gttgatgatg gcgttttcca gttggctgac
    23221 gtcaatccac gcggaccacg ccggagactg cgcttcaatc tcaatcgtca gcgtcgccgg
    23281 taaggagtgg cgcattaatt cgcttaactc ttccagcagt gtttttagtt caacggcttg
    23341 cggatgtaag gactgcttgc gggaaaatgc caacaatcgc tgggtcagta gcgcgccacg
    23401 ttcggcggct tgctgcgccc gctgtattcg cggcgcgtcg ggtgagttcg tgttcaccag
    23461 ttccaggcta ccaataatta ccgccagcag attattaaaa tcatgggcca gcccgcccgt
    23521 cagttgcctg accgctttta acttctggct gtgcaatagc gcctcttcca gcccctgccg
    23581 ctcggtttgg gcaatctcca tctgcgtggt tttctctttc aacagccgtg acgttcgttc
    23641 aagtgatgcg gtattgcggg caaaaacgtt aaaggcgcgc gccagttcac ccaactcatc
    23701 gcgccgctgt aacgccggaa aagtgacgtc cgcttcgccg tgcgccaggc ggctcatcgc
    23761 gcgggaaatc gccgtcaggt tagagccgag attgcgatag atataccaac ctgcaaacgc
    23821 ggtgatcacc accgctaaca gggcgaaaat catgatcagc ctctcgccgg agcgcaaatc
    23881 ctggtaactt tgctccaccc gctgaccaga gagcgtcgcc acctgttcgg cgtaacggtt
    23941 gatgtcgcca ttaaggatcg ccaccagcgc tttgatgtga aacatatacc agctgattgc
    24001 cagatcgctt tgctccagag ccgccgaaag tggcgcgagt ttacgcagtt cacgattaaa
    24061 atcagcgagt agcgtattaa ccaatggcgt atcgctgtga gttggcaagg cagacatcac
    24121 tccgaccagt tgatgaataa tcgcctgcgg cgcgggggtg gcaatggcgg caacaatcag
    24181 tcggttcatc tctttaagca aaatattatc ttgcgcgtga gtgacttttt gcagatgccg
    24241 cagataactt aagttttgat acagcgaact taacagcgcg ttacgctcca gatgccgcct
    24301 ttgcccgcgt tccagcattc cgccgacgct ggtttgcagt tcgttgctac gcgtgatgat
    24361 gcgcgtcacc agcccggctt cctgctgcgc taaaggcgcg ttcgccagtt gtgcaagcga
    24421 gctttttaat gcccgctgtg tctcctgcaa ccgctcggct tcgcctttat attccagtgc
    24481 tcccaccacc tgcgacaatc gcactgccgc cgttgccacg tttgccgtgt cgcgcgccag
    24541 attcaggctg ccggtcatat catctacagt ttgctgcttc accagctctt gcatctggct
    24601 ggcatgacga aagcccatta ccgccacgcc gctgaccatc aacgtcaccg ccaccaccag
    24661 caaattcaaa agcagcaatc gcccgcgcgc gctggcaaaa aagtgacgtc cggggtgcgc
    24721 catatcacgc tccgtgaaga aaaatcgcaa tatgacaaat atgaacggct tctgacattt
    24781 tgcatttact ttcctgtgat ggagtgctga tatcgacaac ctcgcaggag tccaccatga
    24841 acagcacgcc ggttcttgag atgcgcaata ttgccaaagc cttcggcaaa ttttatgcgc
    24901 tcaaaggggt ggatttgacg gtctaccctg gcgagatcca tgccctgatg ggggaaaacg
    24961 gcgcgggaaa aagcacgctg atgaaggtgc tggcgggggc gtataccgcc accagcggcg
    25021 agatcctgat agacggcaag ccctttcaca ttcgcacgcc aaaagatgcc ttaagcgccg
    25081 gtattacgct gatttatcag gagatgcagc tggcacccaa tttgacggtg gcagaaaata
    25141 ttttcctcgg cagcgagctt tcccacggcg ggctggtgca gcgaaaagag atgctggtgc
    25201 aggcgcaaag agtgatcgac cgcctcggcg cgcagtttaa cgccagcgat aaggtcatga
    25261 cgctgaccat tgccgaacaa cagcaggtcg aaatcgcccg cgcgctacat cgcaacagcc
    25321 gcattctggt gatggacgaa cccaccgctg ccctctcctc ccgtgaaact caccgtcttt
    25381 ttgaactgat tatgcggctg cgcgatgaag ggatggcgat tatctacatc agccaccgta
    25441 tggcggaagt gtatgagctt tccgatcgcg tcagcgtgct gcgcgacggg caatacgttg
    25501 gcagcctgac ccgcgataac ctcaatgccg gggagctggt gcggatgatg gtcggcaggc
    25561 cactgagcga tctgttcaat aaagagcgcg atatcccgct cggtaaagcc cgcctgaatg
    25621 ttcaccacct gaccgacggc ggcaaagtcc agccgagctg cctgcaggtg ctttccggcg
    25681 aaattgttgg cctcgccggg ctggtgggag ccggacgttc cgaactggcg cagttaattt
    25741 tcggcgtgcg gaaagcgaca ggcggaatga ttgaagtcga tggcgaaccg gtggtgatcc
    25801 actccccgcg cgaagccatc gatcttggca ttggttttct caccgaaaac cgcaaagaac
    25861 aaggcttatt ccttgaaatg gcggcagtcg aaaacatcac tatggcaacc ctggagcgcg
    25921 atgccagctg gggaatgctc aatcgcaaaa aagcgcaaac catttccgat gacgccatta
    25981 agttgctcaa cattcgcgtg cctcatgccc aggtacgcgc gggcgggctt tccggcggca
    26041 atcagcaaaa actgttgatc tcccgctggg tggcgattgg cccgcgcatt ttactgctcg
    26101 atgaacccac ccgcggcgtg gacgttggcg caaaaagtga gatctaccgg atcatgaacg
    26161 agatggcgcg caagggcgtg gcgatcctga tgatctccag cgaactgccg gagatagtgg
    26221 gaatgagcga tcgcgtccat gtgatgcgcg aaggcagcat tgccggtgag ctaaatggca
    26281 aaaacatcac ccaggaaaac attatgacct tagcgactgg cgtcaacgac gcccattccc
    26341 aggcggtgac ctcatgacga cctctgcaaa aacctctgcc aaaaaaatgc tgatgagcga
    26401 tctgatgcaa acggtcggca ttttgcccat tttgatcctg atcgtagcgg tatttggctt
    26461 tatcgcgcca aactttttta ccgaaagcaa cctgctaaat attacccgcc aggcgtcgat
    26521 caacattgtg ctggcggcgg ggatgacctt catcattttg accggtggga tcgatctttc
    26581 tgtaggttca atcctcggca ccacggcggt ggcggcaatg gttgtctccc ttagcccgga
    26641 gttcgccatg ttgtctatcc cggcggcgct gatgctcggc ctgctgctgg ggctgttcaa
    26701 cggcgcgctg gtggcctttg ccggattgcc gccgtttatc gtcacgcttg gcacctatac
    26761 ggcgctgcgc ggggcggcgt atctgctggc tgatggcacc acggttatca actccaacat
    26821 taatttcgag tggataggca ataactatct cgggccggtc ccgtggctgg tggtgattgc
    26881 gctggcggtg atcgtgctgt gctggtttat tctgcgtcgc accacgcttg gcgttcatat
    26941 ctacgccgtc ggtggcaata tgcaggcggc gcgtcttacc ggcattaagg tctggctggt
    27001 gctgctgttt gtttatggca tgagcggcct gctttcaggg cttggcggga ttatgagcac
    27061 ctcgcggctg tacagcgcca acggcaactt aggtatgggt tacgaactgg atgccattgc
    27121 ggcggtgatc ctcggcggca ccagttttgt cggcggcatc ggcaccatca ccggcacgct
    27181 ggtgggggcg ctaattatcg ccacgctcaa caatggcatg accttaatgg gcgtctctta
    27241 cttctggcag ctggtgatca aaggggcggt gatcatcatt gcggtgctta tcgacaaata
    27301 ccgtacccga catcatcaaa gtgcataaca acaataccct acacatgagg aatacagcat
    27361 gagattgaag cccttagtga ccgcgctctg tgcaggcgcg ctgctggccg cagcaccgtt
    27421 cgcccaggcg aaagatctga aatcgatagg cgtaacggta ggtgacctgg cgaacccgtt
    27481 cttcgtacag atcaccaaag gcgcggagct ggaagcacgc aaactggcgg gcgataacgt
    27541 caaagtgacg ctggtttcca gcggctacga tctgggtcag caggtctcgc agatagacaa
    27601 ctttatcgcc gctaacgtcg atatgatcat ccttaacgct gccgattcaa aagggatcgg
    27661 cccggcggtg aaacgggcaa aagacgccgg gatcgtggtg gtggcggttg acgtggcggc
    27721 agaaggtgcc gatgccacca tcacttccga taacacccaa gcaggcgaaa tggcctgtaa
    27781 atacatcacc gatcgcctga aaggcaaagg caacgtggtg atcatcaacg gaccgccggt
    27841 gtccgccgtg caaaaccgcg tcgaaggctg ccagaccgaa tttaaacgcc atcctgacat
    27901 caaggtgctc tctgacaaca agaacgccaa aggcagtcgt gagggcgggc tggaggtgat
    27961 gaccgggctg ctggcggcaa atccgaagat cgacggcgta tttgcgatta acgatccgac
    28021 agcgatcggt gccgatctgg cagcaaaaca ggcgcaacgt aacgagttct ttattgtcgg
    28081 cgtggatgga agcccggacg ccgaagaggc gctcaagcgt gagaactcgt tgtttgtcgc
    28141 tactccggcg caagatccac aggtgatggc agcgaaagcg gttgagatca gttatgacat
    28201 tttgcagggc aaacctgcgc cgaaagagcc ggtgctgatc ccggtgacga tgatcgataa
    28261 aaacaacgtc ggcaattaca aaggctggac ggtgaaataa gtccacacgc cgggcggcga
    28321 tgctgcccgg ccaccgctaa ggagaacgct ttcgatgaaa cgctccgcta taaacaacat
    28381 tctcggccat acacggcaat tcttttcgca acatgacgta catctgccgc cctttgccag
    28441 tttctctcct gctcaatggc agcagctcga caccgccgcg tgggaggtgg tttttgatct
    28501 caaactcggc tgggatgtta ccgcttttgg tggtaacaac tttgcagcac agaggctgac
    28561 gctgtttacc ctgcgtaacg gctcagcaaa aggaatgccg tatgtgaaat gttacgccga
    28621 gaagatcatg catgtgcgcg atgcgcaggt cacgccaatg cattttcact ggcgtaagcg
    28681 tgaggacatc atcaatcgcg gcggcggcaa tttgattgtt gaactgtgga atgccgatag
    28741 caacgagcaa acagttgata ccgacgttac ggtggtgatc gacggctgcc gccagactca
    28801 tgtcgcaggc agtcagttac gcctgtcccc cggcgaaagt atctgcctgc cgcccggcct
    28861 gtatcacagc ttttgggcgg aaaccggttt tggcgatgtt ctggttggcg aagtctcttc
    28921 tgtcaatgac gacgaccacg ataaccactt tttacaaccg ctggaccgct acaacctgat
    28981 tgacgaagac gaaccggccc agctggtgtt gtgtaacgaa taccgccagt tcctctgaat
    29041 aaggataacg ttatgccgtt gatttctctc gctgaagggc tggcacatgc ccgcgaacat
    29101 cactatgcgt taggtgcgtt taatgtgctg gactcacact ttttacgcgc cttgtttgct
    29161 gctgccagac aagaacgttc gccctttatt atcaacattg ccgaagtaca ttttaaatat
    29221 atctcgctgg attccctggt cgagacagtg aaatttgaag cggcgcgtca agcgattccg
    29281 gtggtattaa atctcgatca cgggctgcat tttgaatcgg tggcgcaagc gttgcggctg
    29341 ggattcagct cggtaatgtt tgatggctcc acgctggggt atgaagagaa tatccgccag
    29401 acgcgggaag tggtgaaaat gtgccatgcc gttggcgtat cggtagaagc tgaactgggc
    29461 gcggtgggtg gtgacgaagg cggtgcgctt tacggtcatg ccgatgaatc tctgtttacc
    29521 gatccgcagc tggcgcgtga ttttgtcgac cgtaccggca ttgacgcgct ggccgtggcg
    29581 attggcaatg cgcacggcaa atatcgcggc gaaccgaaac tcgatttccc gcgcctcgac
    29641 gctatccgcc agcaggcggc tatcccgctg gtattacacg gcggttcagg gattagagat
    29701 gccgatttcc gccgcgccat cgaactgggt atccacaaaa tcaatttcta caccggcatg
    29761 tcacaagcgg cgctggctgc cgttgaggcc cgaatggcgc accgtcagcc gatgtatgac
    29821 gagtttgccg agttactact agcagtggaa gccgccatca gcgatacggt tgcacaacag
    29881 atgcgtattt tcggcagtgc ggggcaggca tgatggaacg taaagggatt atcgccgcag
    29941 gcaatatgct ggttgaccac gtccaccaga ttatccagtg gccagagcgc ggctggctgg
    30001 cggagattac ccacagcgaa cgctccaccg gcggcgcgcc gcttaacgtc ctgctgacac
    30061 tggcgaaaat gcgcaccggg ttgctgttac aggcggtcgg gctaatcggt caggacagcg
    30121 acggcgatta cattatggcg atgcttgagc aatatcacgt caatcgtcag catgtgcagc
    30181 gcactacctt tgccccgacg tcaatgtcgc aggtaatgac tgatccaagc gggcagcgga
    30241 cgtttttcca ttcgccaggt gcaaacagac ttttggatct tccggcgttc gatcagctcg
    30301 ataacaccat gaagattttc catctcggct atttactgtt gctcgacagc ctggatatgc
    30361 cggacgatgt ttacggcacc cgtagcgcac gcctgctggc gcaaatgcgt gatactggct
    30421 ttgaaacctc actcgacctg gtctcccgca agggcgatcc gcgttatcag ccgctggtgt
    30481 tgcccgcgtt gcgccatctg gattacctgg taattaacga actggaggcc ggggagttta
    30541 gcgggctgga aattcggtta cccaacggcg aaccgcatat cgcgcatatt gctgccgccg
    30601 cgcgtgaact gttagatgcg ggcgtgcgcc agcgtgtggt gatccactgc ccggaagggg
    30661 cgtggggaga gaccccggaa cagtgcggcg tgtggatccc ttcgcaaaag ttagaacaac
    30721 gggaaattat cggcagcgtc ggtgcgggcg atgtattttg tgccggattt ttatatggct
    30781 gccatgaacg ctggacgctt accgagagca tcaaactggc acacgcctgc gcgcgagcaa
    30841 gcctgttatg cgccaatgcc atcgacgggg cgaaaacgct ggaagagctg caaacagaaa
    30901 tgtcggatta atgcacgtcg gcagcgaaga cataccccag accgcgcaga gttttaatca
    30961 acatcggctg atgcgggttg agttcgattt tgcgtcgtaa gcgcatgatt aatacgtcga
    31021 tggtgcgatc aaacacctcc acgctgtcgc tatgagtgaa cgccagcagc tgttcgcggc
    31081 ttaatacccg acgggcattt tgagccaggg ccagtaacaa gccatattcg ccctgagtca
    31141 gctcgacatg ttgttgtagc ggactatgca gttggcagcg aatggtatcg agataccagc
    31201 cattgaatga cagcccggcg tttttctcct cattaaccgc cagcgcgcca acccgtcgca
    31261 acacgctttc agtcgtgcga ccaccacccg tggggtgaac ggcttgcaga tgtaatcatc
    31321 cgcgcccatt tccagcccga ccaccacatc agactcactc cccattccgg tcaacatcac
    31381 cacaggcaat gccggacgca gtttctggat ttgctgcaat accctcaggc cgttggtatc
    31441 gggcagcatc atatccagca ccaccagcgc aatgtcaggc tcgccctcaa tacgcgatat
    31501 ggcctcctgt ccggtatggc tgactgaaac cgtaaagaca tgttcactta acacgtcctg
    31561 caacagtgcg cagatagccg tgtcatcatc caccaccagc acgacgggtt tcatggttac
    31621 actccgcatc attcatctaa ttaatcattg tgcgtacttc ttgtcgaagt gaaagcggaa
    31681 agtaatataa ataataaaaa tataagtcag gtcacattat tgatgatagt aataaatatg
    31741 ccgtgctttt ccctcttcag cacaaatgcc taataaaatg gcgcgttccg ctcacacttc
    31801 cacttattaa agtcgcaaat tcccaattat gataacccca gctttaaatg acgtattttg
    31861 catggattac atatttacga cacaaatgat aaatggagat ttatatgaat aactctcggt
    31921 tattccgttt gagcagaatt gttattgcgt taactgccgc cagcggcatg atggtaaata
    31981 ccgctaacgc gaaagaggaa gcgaaagccg ccactcaata tacccaacag gttaatcaga
    32041 attacgccaa atcattaccg tttagcgatc gtcaggattt tgacgacgct cagcgtggat
    32101 ttatcgctcc gctgctggat gaaggtattc tgcgcgatgc taatggcaaa ccatattatc
//
LOCUS       DADLTF010000034        30372 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.034, whole genome shotgun sequence.
ACCESSION   DADLTF010000034 DADLTF010000000
VERSION     DADLTF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.034"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..378
                     /locus_tag="JN749_001458"
     CDS             <1..378
                     /locus_tag="JN749_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7367920.1"
                     /translation="RAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILS
                     KDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDD
                     GLRFAIREGGRTVGAGVVAKVLS"
     gene            608..991
                     /gene="secE"
                     /locus_tag="JN749_001459"
     CDS             608..991
                     /gene="secE"
                     /locus_tag="JN749_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAY7367921.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            993..1538
                     /gene="nusG"
                     /locus_tag="JN749_001460"
     CDS             993..1538
                     /gene="nusG"
                     /locus_tag="JN749_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAY7367922.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1697..2125
                     /gene="rplK"
                     /locus_tag="JN749_001461"
     CDS             1697..2125
                     /gene="rplK"
                     /locus_tag="JN749_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAY7367923.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2129..2833
                     /gene="rplA"
                     /locus_tag="JN749_001462"
     CDS             2129..2833
                     /gene="rplA"
                     /locus_tag="JN749_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAY7367924.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3246..3743
                     /gene="rplJ"
                     /locus_tag="JN749_001463"
     CDS             3246..3743
                     /gene="rplJ"
                     /locus_tag="JN749_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAY7367925.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3810..4175
                     /gene="rplL"
                     /locus_tag="JN749_001464"
     CDS             3810..4175
                     /gene="rplL"
                     /locus_tag="JN749_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAY7367926.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4495..8523
                     /gene="rpoB"
                     /locus_tag="JN749_001465"
     CDS             4495..8523
                     /gene="rpoB"
                     /locus_tag="JN749_001465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAY7367927.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGELVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8600..12823
                     /gene="rpoC"
                     /locus_tag="JN749_001466"
     CDS             8600..12823
                     /gene="rpoC"
                     /locus_tag="JN749_001466"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAY7367928.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="JN749_001467"
     CDS             complement(13065..14198)
                     /gene="thiH"
                     /locus_tag="JN749_001467"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HAY7367929.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14195..15040)
                     /gene="thiG"
                     /locus_tag="JN749_001468"
     CDS             complement(14195..15040)
                     /gene="thiG"
                     /locus_tag="JN749_001468"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HAY7367930.1"
                     /translation="MSSGRNISCRMATRSCFFRLLQGVEMLRIVDKTFDSHLFTGTGK
                     FASSQLMVEAIRASGSQLVTLAMKRVDLRQHNDAILEPLIAAGVALLPNTSGAKTAEE
                     AIFAAHLAREALGTNWLKLEIHPDARWLLPDPIETLKAAETLVQQGFVVLPYCGADPV
                     LCKRLEEVGCAAVMPLGAPIGSNQGLETRAMLEIIIQQATVPVVVDAGIGVPSHAAQA
                     LEMGADAVLVNTAIAVADDPVNMAKAFRLAVEAGLLARQSGPGSRSYFAHATSPLTGF
                     LEASA"
     gene            complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="JN749_001469"
     CDS             complement(14967..15167)
                     /gene="thiS"
                     /locus_tag="JN749_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HAY7367931.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="JN749_001470"
     CDS             complement(15151..15906)
                     /gene="thiF"
                     /locus_tag="JN749_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HAY7367932.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTADINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="JN749_001471"
     CDS             complement(15899..16534)
                     /gene="thiE"
                     /locus_tag="JN749_001471"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HAY7367933.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="JN749_001472"
     CDS             complement(16534..18429)
                     /gene="thiC"
                     /locus_tag="JN749_001472"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HAY7367934.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIHRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18510..18610)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="JN749_001473"
     CDS             complement(18663..19139)
                     /gene="rsd"
                     /locus_tag="JN749_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HAY7367935.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19234..20007
                     /gene="nudC"
                     /locus_tag="JN749_001474"
     CDS             19234..20007
                     /gene="nudC"
                     /locus_tag="JN749_001474"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HAY7367936.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20047..21111
                     /gene="hemE"
                     /locus_tag="JN749_001475"
     CDS             20047..21111
                     /gene="hemE"
                     /locus_tag="JN749_001475"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HAY7367937.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21268..21939
                     /gene="nfi"
                     /locus_tag="JN749_001476"
     CDS             21268..21939
                     /gene="nfi"
                     /locus_tag="JN749_001476"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HAY7367938.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21982..22572
                     /locus_tag="JN749_001477"
     CDS             21982..22572
                     /locus_tag="JN749_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HAY7367939.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22759..23031
                     /gene="hupA"
                     /locus_tag="JN749_001478"
     CDS             22759..23031
                     /gene="hupA"
                     /locus_tag="JN749_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HAY7367940.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23044..23739
                     /locus_tag="JN749_001479"
     CDS             23044..23739
                     /locus_tag="JN749_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HAY7367941.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRASFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="JN749_001480"
     CDS             complement(23741..24166)
                     /gene="zraP"
                     /locus_tag="JN749_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HAY7367942.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24404..25780
                     /gene="zraS"
                     /locus_tag="JN749_001481"
     CDS             24404..25780
                     /gene="zraS"
                     /locus_tag="JN749_001481"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HAY7367943.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25777..27102
                     /gene="zraR"
                     /locus_tag="JN749_001482"
     CDS             25777..27102
                     /gene="zraR"
                     /locus_tag="JN749_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HAY7367944.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27099..28388)
                     /gene="purD"
                     /locus_tag="JN749_001483"
     CDS             complement(27099..28388)
                     /gene="purD"
                     /locus_tag="JN749_001483"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAY7367945.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28400..29989)
                     /gene="purH"
                     /locus_tag="JN749_001484"
     CDS             complement(28400..29989)
                     /gene="purH"
                     /locus_tag="JN749_001484"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAY7367946.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7111 a         7914 c         8360 g         6987 t
ORIGIN      
        1 cgtgctggtg agaacgtagg tgttctgctg cgtggtatca aacgtgaaga aatcgaacgt
       61 ggtcaggtac tggctaagcc gggcaccatc aagccgcaca ccaagttcga atctgaagtg
      121 tacattctgt ccaaagatga aggcggtcgt catactccgt tcttcaaagg ctaccgtccg
      181 cagttctact tccgtactac tgacgtgact ggtaccatcg aactgccgga aggcgtagag
      241 atggtaatgc cgggcgacaa catcaaaatg gttgttaccc tgatccaccc gatcgcgatg
      301 gacgacggtc tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta
      361 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
      421 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      481 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      541 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      601 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      661 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      721 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      781 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      841 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      901 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      961 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
     1021 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
     1081 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
     1141 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
     1201 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
     1261 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
     1321 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1381 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1441 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1501 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1561 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1621 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1681 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1741 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1801 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1861 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1921 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1981 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     2041 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     2101 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     2161 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     2221 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     2281 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     2341 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2401 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2461 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2521 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2581 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2641 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2701 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2761 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2821 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2881 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2941 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     3001 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     3061 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     3121 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     3181 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     3241 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     3301 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3361 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3421 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3481 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3541 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3601 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3661 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3721 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3781 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3841 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3901 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3961 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     4021 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     4081 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     4141 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     4201 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     4261 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     4321 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4381 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4441 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4501 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4561 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4621 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4681 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4741 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4801 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4861 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4921 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4981 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     5041 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     5101 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     5161 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     5221 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     5281 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     5341 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5401 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5461 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5521 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5581 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5641 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5701 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5761 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5821 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5881 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5941 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     6001 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     6061 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     6121 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     6181 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     6241 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     6301 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6361 ctggatgaag gaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6421 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6481 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6541 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6601 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6661 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6721 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6781 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac tggttgaacg tggcgacgtg
     6841 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6901 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6961 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     7021 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     7081 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     7141 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     7201 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     7261 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     7321 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7381 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7441 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7501 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7561 cacgagttcg agaagaaact cgaagcgaaa cgccgaaaaa tcacccaggg cgacgatctg
     7621 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7681 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7741 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7801 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7861 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7921 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7981 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     8041 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     8101 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     8161 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     8221 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     8281 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     8341 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8401 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8461 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8521 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8581 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8641 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8701 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8761 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8821 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8881 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8941 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     9001 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     9061 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     9121 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     9181 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaactaagcg
     9241 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     9301 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9361 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9421 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9481 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9541 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9601 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9661 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9721 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9781 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9841 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9901 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9961 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
    10021 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
    10081 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
    10141 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
    10201 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
    10261 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
    10321 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10381 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10441 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10501 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10561 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10621 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10681 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10741 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10801 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10861 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10921 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10981 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    11041 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    11101 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    11161 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    11221 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    11281 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    11341 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11401 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11461 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11521 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11581 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11641 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11701 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11761 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11821 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11881 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11941 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    12001 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagaaccggc
    12061 aatcttggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    12121 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    12181 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    12241 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    12301 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12361 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12421 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12481 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12541 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12601 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12661 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12721 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12781 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12841 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12901 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12961 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    13021 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    13081 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    13141 gcggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    13201 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    13261 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    13321 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13381 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13441 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13501 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13561 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13621 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13681 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13741 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13801 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13861 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13921 ccgcagtacg tgcagttgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13981 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    14041 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    14101 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    14161 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    14221 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    14281 gcaggcctgc ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    14341 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat
    14401 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14461 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14521 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14581 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14641 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14701 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14761 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14821 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14881 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14941 acgttttgtc cacaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    15001 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    15061 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    15121 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    15181 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    15241 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    15301 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15361 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15421 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15481 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15541 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15601 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15661 cgggctgttg atatctgcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15721 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15781 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15841 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15901 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15961 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    16021 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    16081 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    16141 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    16201 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    16261 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    16321 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16381 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16441 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16501 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16561 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16621 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16681 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16741 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16801 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16861 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16921 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16981 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    17041 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggtg
    17101 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    17161 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    17221 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    17281 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    17341 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17401 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17461 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17521 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17581 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17641 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17701 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17761 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17821 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17881 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17941 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    18001 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    18061 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    18121 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    18181 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    18241 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    18301 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18361 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18421 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18481 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18541 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18601 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18661 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18721 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18781 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18841 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18901 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18961 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    19021 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    19081 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    19141 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    19201 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    19261 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    19321 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19381 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19441 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19501 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19561 accgaatggg cgatgctgtg cagccattgc cgtgagcgtt actacccgca aatcgccccc
    19621 tgcattattg ttgccatccg tcgcgatgat tcgatcctcc tcgcccagca tacccgccat
    19681 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19741 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19801 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19861 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19921 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19981 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    20041 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    20101 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    20161 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    20221 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    20281 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    20341 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20401 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20461 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20521 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20581 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20641 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20701 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20761 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20821 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20881 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20941 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    21001 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    21061 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    21121 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    21181 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    21241 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    21301 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21361 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21421 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21481 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21541 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21601 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21661 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21721 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21781 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21841 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21901 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21961 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    22021 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    22081 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    22141 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    22201 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    22261 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    22321 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22381 caggctggtc gcgaaatgag cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22441 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22501 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22561 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22621 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22681 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22741 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22801 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22861 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22921 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22981 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    23041 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    23101 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    23161 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    23221 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    23281 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    23341 ccgccggtga acattcgcgc gagttttgac gatcgcggcg atctgagctt tatgcaacgt
    23401 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23461 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23521 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23581 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23641 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23701 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23761 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23821 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23881 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23941 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    24001 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    24061 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    24121 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    24181 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    24241 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    24301 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24361 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24421 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24481 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24541 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24601 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24661 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24721 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24781 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24841 ttatcgccag tttcaaccga tgtttgctgc tggaatgcac cggatgcgcc atatgcagca
    24901 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24961 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    25021 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    25081 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    25141 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    25201 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    25261 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    25321 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25381 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25441 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25501 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25561 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25621 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25681 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25741 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25801 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25861 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25921 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25981 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    26041 gtcgagacgg cggtagaggc actgaaaact ggggcgctgg attatctcat caagccgctg
    26101 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    26161 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    26221 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    26281 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    26341 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26401 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26461 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26521 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26581 cagactatct cggttgatgt ccggctgatt gcggcgactc atcgcgatct tgccgcagag
    26641 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26701 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26761 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26821 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26881 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26941 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    27001 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    27061 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    27121 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    27181 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    27241 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    27301 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27361 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27421 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27481 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27541 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27601 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27661 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27721 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27781 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27841 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27901 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27961 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    28021 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    28081 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    28141 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    28201 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    28261 agcgcgggtt ccagcgcagt gcctgcatta cccggagcaa caaaaacagt ctcaaccagc
    28321 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28381 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28441 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28501 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28561 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28621 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28681 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28741 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28801 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28861 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28921 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28981 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    29041 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    29101 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    29161 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    29221 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    29281 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    29341 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29401 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29461 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29521 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29581 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29641 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29701 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29761 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29821 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29881 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29941 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatccct
    30001 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    30061 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    30121 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    30181 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    30241 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    30301 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30361 ttcggttgaa ga
//
LOCUS       DADLTF010000035        29932 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.035, whole genome shotgun sequence.
ACCESSION   DADLTF010000035 DADLTF010000000
VERSION     DADLTF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.035"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            227..1675
                     /locus_tag="JN749_001485"
     CDS             227..1675
                     /locus_tag="JN749_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAY7367947.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1677..1802
                     /locus_tag="JN749_001486"
     CDS             1677..1802
                     /locus_tag="JN749_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367948.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1799..1870)
                     /locus_tag="JN749_001487"
     CDS             complement(1799..1870)
                     /locus_tag="JN749_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367949.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            1925..2473
                     /gene="idi"
                     /locus_tag="JN749_001488"
     CDS             1925..2473
                     /gene="idi"
                     /locus_tag="JN749_001488"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAY7367950.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            complement(2516..4033)
                     /gene="lysS"
                     /locus_tag="JN749_001489"
     CDS             complement(2516..4033)
                     /gene="lysS"
                     /locus_tag="JN749_001489"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAY7367951.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4043..5141)
                     /gene="prfB"
                     /locus_tag="JN749_001490"
     CDS             complement(join(4043..5065,5067..5141))
                     /gene="prfB"
                     /locus_tag="JN749_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAY7367952.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5232..6965)
                     /gene="recJ"
                     /locus_tag="JN749_001491"
     CDS             complement(5232..6965)
                     /gene="recJ"
                     /locus_tag="JN749_001491"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAY7367953.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(6971..7681)
                     /gene="dsbC"
                     /locus_tag="JN749_001492"
     CDS             complement(6971..7681)
                     /gene="dsbC"
                     /locus_tag="JN749_001492"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HAY7367954.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7706..8602)
                     /gene="xerD"
                     /locus_tag="JN749_001493"
     CDS             complement(7706..8602)
                     /gene="xerD"
                     /locus_tag="JN749_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAY7367955.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8714..9235
                     /gene="fldB"
                     /locus_tag="JN749_001494"
     CDS             8714..9235
                     /gene="fldB"
                     /locus_tag="JN749_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HAY7367956.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9275..9682)
                     /locus_tag="JN749_001495"
     CDS             complement(9275..9682)
                     /locus_tag="JN749_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367957.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9663..9929)
                     /gene="sdhE"
                     /locus_tag="JN749_001496"
     CDS             complement(9663..9929)
                     /gene="sdhE"
                     /locus_tag="JN749_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HAY7367958.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            10172..11152
                     /gene="ygfZ"
                     /locus_tag="JN749_001497"
     CDS             10172..11152
                     /gene="ygfZ"
                     /locus_tag="JN749_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HAY7367959.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11348..12007)
                     /locus_tag="JN749_001498"
     CDS             complement(11348..12007)
                     /locus_tag="JN749_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAY7367960.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12171..12482)
                     /locus_tag="JN749_001499"
     CDS             complement(12171..12482)
                     /locus_tag="JN749_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAY7367961.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            12527..13959
                     /locus_tag="JN749_001500"
                     /pseudo
     CDS             12527..13959
                     /locus_tag="JN749_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            complement(14016..14759)
                     /locus_tag="JN749_001501"
     CDS             complement(14016..14759)
                     /locus_tag="JN749_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7367962.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15026..17899)
                     /gene="gcvP"
                     /locus_tag="JN749_001502"
     CDS             complement(15026..17899)
                     /gene="gcvP"
                     /locus_tag="JN749_001502"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HAY7367963.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18018..18407)
                     /gene="gcvH"
                     /locus_tag="JN749_001503"
     CDS             complement(18018..18407)
                     /gene="gcvH"
                     /locus_tag="JN749_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HAY7367964.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18431..19525)
                     /gene="gcvT"
                     /locus_tag="JN749_001504"
     CDS             complement(18431..19525)
                     /gene="gcvT"
                     /locus_tag="JN749_001504"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HAY7367965.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLIVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            19816..19899
                     /locus_tag="JN749_001505"
     CDS             19816..19899
                     /locus_tag="JN749_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7367966.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            complement(19973..21175)
                     /gene="ubiI"
                     /locus_tag="JN749_001506"
                     /gene_synonym="visC"
     CDS             complement(19973..21175)
                     /gene="ubiI"
                     /locus_tag="JN749_001506"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HAY7367967.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(21198..22376)
                     /gene="ubiH"
                     /locus_tag="JN749_001507"
                     /gene_synonym="visB"
     CDS             complement(21198..22376)
                     /gene="ubiH"
                     /locus_tag="JN749_001507"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HAY7367968.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22373..23698)
                     /gene="pepP"
                     /locus_tag="JN749_001508"
     CDS             complement(22373..23698)
                     /gene="pepP"
                     /locus_tag="JN749_001508"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HAY7367969.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23724..24302)
                     /gene="ygfB"
                     /locus_tag="JN749_001509"
     CDS             complement(23724..24302)
                     /gene="ygfB"
                     /locus_tag="JN749_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HAY7367970.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24470..24799
                     /gene="zapA"
                     /locus_tag="JN749_001510"
     CDS             24470..24799
                     /gene="zapA"
                     /locus_tag="JN749_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAY7367971.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24841..25024
                     /gene="ssrS"
                     /locus_tag="JN749_001511"
     ncRNA           24841..25024
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="JN749_001511"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25045..25647
                     /locus_tag="JN749_001512"
     CDS             25045..25647
                     /locus_tag="JN749_001512"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAY7367972.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25744..25803)
                     /locus_tag="JN749_001513"
     CDS             complement(25744..25803)
                     /locus_tag="JN749_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7367973.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26136..26195)
                     /gene="ibsC"
                     /locus_tag="JN749_001514"
     CDS             complement(26136..26195)
                     /gene="ibsC"
                     /locus_tag="JN749_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HAY7367974.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26424..27656)
                     /locus_tag="JN749_001515"
     CDS             complement(26424..27656)
                     /locus_tag="JN749_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAY7367975.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(27912..28571)
                     /gene="rpiA"
                     /locus_tag="JN749_001516"
     CDS             complement(27912..28571)
                     /gene="rpiA"
                     /locus_tag="JN749_001516"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAY7367976.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            28954..29847
                     /locus_tag="JN749_001517"
     CDS             28954..29847
                     /locus_tag="JN749_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HAY7367977.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
BASE COUNT         7112 a         8061 c         7505 g         7254 t
ORIGIN      
        1 gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca ttgtcaggtt
       61 ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat tcctgtctct
      121 aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca gtttcattaa
      181 cgtaaacggt tgctttttat tctggcgggc gaaaggagaa acactgatga gcgccataga
      241 ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg accgcatatt
      301 atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt acgcaggtgc
      361 agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg ctattgcgat
      421 gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat gtatcggtat
      481 cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg ctgctgtaac
      541 accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat ttggtgccac
      601 tatcgccgcg ggttttatca ccacattatt agcgccactt attggtcgct tgatgccttt
      661 attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca tcattcaggt
      721 gggtattgac tgggccgctg gaggtaaagg gaatccgcaa tatggtaatc ccgtttattt
      781 aggtatctcc tttgccgtct taatttttat cttgctcatt actcgctatg cgaaaggatt
      841 tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt cgtggatgat
      901 gaatgaagtc aatttatccg ggctacatga tgcctcgtgg tttgcgattg tcaccccgat
      961 gtcgtttggt atgccgattt tcgatcccgt ttccattctg accatgactg ccgtgttaat
     1021 catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag tcggtcgtaa
     1081 actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga caatgatagg
     1141 cggcacgttt aacagcttcc cccacacgtc attttctcaa aacgttggcc tggttagcgt
     1201 gatgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa tcctgtttgg
     1261 catggtgcca aaaatggcgg tgctggtagc ctccattccg caatttgtgc tgggcggcgc
     1321 tggtctagtg atgttcggca tggtactggc gacagggatt cgaattctgt cgcgctgtaa
     1381 ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg ttggcatgac
     1441 tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac cgctgctaca
     1501 tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aacgtcttct ttaatggcta
     1561 tcagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa aagtcaggac
     1621 agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag aataatatga
     1681 attttttaat gcgcgctata ttcagtctgt tgttgctttt tactctctct attcctgtca
     1741 tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg ctactttttt
     1801 aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa acgctctctt
     1861 ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt acatgtgaga
     1921 aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca cgggtacgct
     1981 ggaaaagtat gccgcacaca cggcagacac cctcttacat ctcgcgttct ccagttggct
     2041 gtttaatgcc aaggggcaat tattagttac ccgccgcgca ctgagcaaaa aagcgtggcc
     2101 tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca gcgaagacgc
     2161 ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg aatctatcta
     2221 tcctgacttt cgctaccgcg ccaccgatcc gagtggcatt gtggaaaatg aagtgtgtcc
     2281 ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag tgatggatta
     2341 tcaatggtgt gatttagctg atgtattacg cggtattgat gccacgccgt gggcgttcag
     2401 tccgtggatg gtgatgcagg cgacaaatcg cgaagccaga atacgattat ctgcatttac
     2461 ccagcttaaa taaaaaaccc cgacatttgc cggggttgtg agcataacgt aatgcttatt
     2521 tcaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg ttggtgaaca
     2581 gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag ccatgttcca
     2641 gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg tctttcgcgg
     2701 caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg ctaaagccgt
     2761 taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc gggttaatgt
     2821 cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag gtcggctgaa
     2881 tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc agaccccagc
     2941 tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg aagttatcca
     3001 gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg gtcagttttt
     3061 cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc ttaccgagaa
     3121 tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct ttgtaatctg
     3181 cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg gaaatacctt
     3241 cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc accagacgct
     3301 tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg ttatggtggg
     3361 tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc gtttcaactt
     3421 ccataaagcc acggttcacc atgaactggc gaataccaga gaggatctgc gagcgtactt
     3481 taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga cgatagcgcg
     3541 cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct ttggtcagca
     3601 gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac agtttacctt
     3661 tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa acgccttccg
     3721 gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc agggtaacga
     3781 aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg acttcgatgt
     3841 tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat tggtcagagg
     3901 tatgatcgcg acggaaatcg ttcgggaagg caattccctg ctcacgcagg ttcgccagct
     3961 tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg ccctgtgcgt
     4021 gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg ataaattgat
     4081 ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggtg cgcagatctt
     4141 taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg atgtcagatt
     4201 tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt tcataaagct
     4261 tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg tcgttctggc
     4321 actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg gtacggttaa
     4381 cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc gccgggttga
     4441 tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg aacgacgtgt
     4501 ggcgacgacc gccggagtca aacgggcttt tacgcaccag gcggtgaacg cccgtttctg
     4561 tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat ttaatacccg
     4621 ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat tctgcccagc
     4681 gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg ccagaccccg
     4741 cctgaatatc gaggtaacag tcggcgctgt catattcgcc agagaacata cggcggaact
     4801 caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg ttaaaggttt
     4861 cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc agcccctgtt
     4921 tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta cccagcgcct
     4981 gtgcgcgttc gggttcgttc cagacatccg gctgttccag ctcggcgttt acttcttcca
     5041 gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga gcgttccgtg
     5101 aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt tcttttattg
     5161 agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc ttttttatag
     5221 agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct gcggttgccg
     5281 cgaaactcgt tgatatcgag cttataagcc agttgcactt cgcggacgcc gttatccggc
     5341 cagagagcgg tatcgacatt aaaagcaata ccatccagca gtggaccgcc gccgaccggt
     5401 tcgaccatca ccttcaaatg acgttcgccc accagccgct gttgcagcag acggaaatga
     5461 ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg cagcagctgc
     5521 gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac ttcgccttgc
     5581 aatagcgaag gggccagcca ctcagtaacc agttcgccaa accgttgttg aaagagttcg
     5641 aatttatcct cttccagcga caaacccgcc gccatcgcat gaccgccaaa cttgagcatc
     5701 atgccggggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag cccctgaatg
     5761 gagcgaccgg aacctttcag cgtaccgtca cctgctggcg caaaggcgat aaccggacgg
     5821 tgaaaacgct ctttgatacg cgaagccaga ataccgacaa cgccctgatg ccattcgggg
     5881 tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt ctcgcacagg
     5941 gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt tagcgcatcg
     6001 agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaacag cgccacacca
     6061 acggacatat catccagtcg cccggcagca ttgagacgcg gtcccagcgc aaaacctaaa
     6121 tcgctggcgg ctagtttttg tgcatcgcga tttgccactt ccagcagcgc tttaatccct
     6181 ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat gcgattatta
     6241 gcgtccagcg gcacgacgtc cgctactgtc cccagcgcta ccagatccag cagttctgcc
     6301 aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa aaaggtgcgc
     6361 agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga cgggaaatta
     6421 cagtcgcgca agttagggtt aatgatcgct tccgctgcgg gtaatgtgtc gcccggcaaa
     6481 tggtgatcgg taacaataac cgggatgccc aacgagcgag cgtgctcaac tcccgcatgg
     6541 gaggaaatac cgttatccac cgtgacaatt aactgcgcgc cacgggcatg ggcctgatcg
     6601 accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggtaccag gtagtcgata
     6661 ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt cgcgccgtcg
     6721 gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaagcgtt gtaaaggatc
     6781 tcaacggcct tttcgacacc gcttagttgc tgccagggca acataccttt aacactgcgt
     6841 tccagttctt gcgcactgcg tactccccgg ctggcgtata aacggcgcag caagggaggc
     6901 aattcagcgg gcaagtctgc cgtttcatcg acttcacggc gacgaagttg tatctgttgt
     6961 ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat tctttcatgt
     7021 ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact gccggagtac
     7081 cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg tcgcagctgg
     7141 ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta tctttcgcac
     7201 accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc gggaaagcaa
     7261 gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc agtttgtggc
     7321 agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc ggcgctttat
     7381 aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta ttggtgacat
     7441 tgaccggagc cgtgccacta acgtcataca ttggcccctg aatgatatgt ttaccatcat
     7501 cggtgatgta caacacaccg ctgttagtca gaactgtctt catgccagct acgggcgcgg
     7561 gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt gccgcgtcat
     7621 cagcctgagc aaagcctgaa aacgccgcta acaaagtaaa caacataaaa cctttcttca
     7681 taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga tgaagttgcc
     7741 gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg ctgtggccca
     7801 gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg gcaaaagcgt
     7861 gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg gcataatgtt
     7921 taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg ggaaacaaca
     7981 cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag gtttccagcc
     8041 agtaaaccgc ctcttcccct aacggcacca ggcgctcttt gttgccttta ccaatgaccc
     8101 gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt tcagagacac
     8161 gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc agtggctgat
     8221 caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct tttggcaaac
     8281 gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga aacttttcgc
     8341 gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag ctggtggctt
     8401 tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt tgcgccgtcg
     8461 ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg cgacggtaag
     8521 cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga aactgctcga
     8581 tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat ccattatgcc
     8641 ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac agcagaatcg
     8701 agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt acaccgaaat
     8761 ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac ataacctcaa
     8821 ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta tcccgacctg
     8881 ggattttggt gaaatccaag aagactggga agccgtctgg gatcagctcg acgacctgaa
     8941 ccttgaaggt aaaattattg cgctgtatgg gcttggcgat caactgggat acggcgagtg
     9001 gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg tgaagttcgt
     9061 cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga ttgctgacgg
     9121 gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta gcgacgagcg
     9181 tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg cctgatctca
     9241 ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct tgttgcaaca
     9301 aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc agccataaat
     9361 gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta atcatccacg
     9421 gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc atcaacaagc
     9481 gaatttcccc ctggcgagca ttaatacacc gctggctgcg aacgcaatca aataccacca
     9541 gcgaaagtaa caccatccat aacggggtgt aactgagcgg ccagggcatg agtaaaataa
     9601 cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag gagacgcgca
     9661 aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg gaccattatt
     9721 tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag gtccggatcg
     9781 tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct gtcgtactca
     9841 tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg gcatgcccaa
     9901 tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa tccagtaccc
     9961 ggctattgta acgtgttttt cgacttcttt tacgggaata tcagtaaaca ccatcgcgat
    10021 cgcgaaatta attcacaaca attcaagggc ttcatttttt tggcagtcgc ctcgcagaag
    10081 gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc attagtcatt
    10141 aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc ctccacgtca
    10201 gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact gggcgcttgc
    10261 caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag cagatgtcag
    10321 ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag gtaaaatgtg
    10381 gagcaattta cgtctgttcc gcgacggcga tggctttgca tggattgaac ggcgcagcgt
    10441 gcgtgaaccg cagctgactg aactgaaaaa atatgcggta ttctctaaag tgaccatcgc
    10501 gccagacgac gagcgtgtgc tgcttggtgt tgccggtttt caggcgcgcg ccgcgctggc
    10561 aaatctcttt agcgaactgc cttcgaaaga aaaacaggta gtcaaagaag gcgcgaccac
    10621 tttgctatgg tttgaacacc cggcagaacg tttcctgatc gtaaccgatg aagctactgc
    10681 taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc aacagtggct
    10741 tgcattaaac attgaagctg gtttcccggt gattgatgcc gccaacagcg ggcagtttat
    10801 cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag gctgttatac
    10861 cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aacaaacgcg cgctctggtt
    10921 gctggcaggt agtgccagcc gactgccgga agctggtgaa gacttagagc tgaaaatggg
    10981 cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag atggtcaggt
    11041 cgtggtacag gtcgtcatga ataacgatat ggaaccggac agcatcttcc gcgtacgcga
    11101 cgatgcgaat acactgcata tcgagccgct gccgtattcg ctcgaagagt aaatctctgt
    11161 atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg tctgggattt
    11221 gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc acgccgcatc
    11281 cggcggttgc gctccgatgc ctgatgcgac gctgacgcgt cttatcaggc ctacaaaggc
    11341 atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac acactaccgc
    11401 cgagcacgaa gccgtgccag atggcatggt tgtatggaat gcgtttgcag acgtagaaaa
    11461 tcaccccgag cgaataaacc acgccgccta ccgccagtaa ggtaacgctg cccgccgcga
    11521 gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc agataagtca
    11581 ccagagataa aattttgaat cggtgcgcga tggtcagttt gaacagaatc cccagcaatg
    11641 ctaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc cccaccagca
    11701 aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca aatttcttca
    11761 gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg aggaacagca
    11821 ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg gcattgagat
    11881 ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccagc ccaatgccgt
    11941 gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg aggggcttct
    12001 gaaccataac ttactccaga gaaacgtaca cgcacatgta tacctctcca gcgtaactga
    12061 gaatagttcc agtgaacacc tgttagctaa aataaatttt gatttgagac taatctccta
    12121 aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat ttaaagacat
    12181 ttaaattcaa tcatataaaa ttgtgtctga ccgggataga tgtcagcaat gacctttttc
    12241 agttcaggca gggtcatatt ttcctgctct gcatgttttt ccgtcagtgt atccagcgtt
    12301 acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc aaaacgtccg
    12361 acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat ggtgatggtt
    12421 ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc gtttggctgc
    12481 atggtactat ttcctgtaag aattgactga tctggagcct atgatagtga aaaaactcac
    12541 cttaccgaaa gatttcttat ggggtggcgc cgttgccgct catcaggtcg aaggcggctg
    12601 gaacaaaggc ggcaaaggac cgagcatttg tgacgttctg accggtggcg cacacggcgt
    12661 gccgcgcgaa atcaccaaag aagtcttgcc aggaaaatac tatccaaacc atgaagccgt
    12721 tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg gcttcaaatg
    12781 ttttcggact tccatcgcct ggacgcgtat ttttccaaaa ggtgatgaag ctcagccaaa
    12841 cgaagaaggg ctgaagtttt acgactctct gttcgatgaa ctgctgaaat acaacatcga
    12901 accggtgata accctctccc actttgaaat gccgctgcat ctggtgcagc aatacggtag
    12961 ctggaccaac cgtaaattcg ttgatttctt tgtccgtttc gcggaagtgg tattcgaacg
    13021 ctacaagcat aaagtcaaat actggatgac cttcaacgaa ataaacaacc agcgtaactg
    13081 gcgtgcgccg ctgtttggtt actgctgctc cggcgtggtg tataccgagc atgacaaccc
    13141 ggaagagacg atgtaccagg tgctgcatca ccagtttgtc gccagcgccc tggcagtgaa
    13201 agccgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga tggtgccgct
    13261 ctatccctac tcatgtaacc cggacgatgt gatgttcgct caggagtcga tgcgcgaacg
    13321 ctacgtcttt accgatgtgc agctatgcgg ctattacccg tcctatgtat tgaacgagtg
    13381 ggaacgtcgc ggatttaata tcaaaatgga agacggcgat ctcgatgtac tgcgcgaagg
    13441 cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg ccgaaggcgg
    13501 caccggcgat gcgatctctg gttttgaagg cagcgtaccg aacccgtatg ttaaagcgtc
    13561 tgactggggc tgcagattga tccagtaggt ctgcgctatg ccctttgcga actgtatgag
    13621 cgttatcaga ggccgctgtt tattgtcgaa aacggttttg gcgcttacga caaagtggaa
    13681 gaagatggca gcatcaacga cgactaccgc attgactacc tgcgcgccca tattgaagag
    13741 atgaaaaaag cagtgactta cgatggtgtg gatctgatgg gctacacacc gtggggctgc
    13801 atcgactgcg tgtcattcac taccgggcag tacagcaaac gctacggctt tatctatgtg
    13861 aataaacatg acgacggtac tggcgatatg tcgcgttcac gtaagaagag ctttaactgg
    13921 tacaaagagg tgattgccag caacggcgag aagctttaag tcgatgaagt accggatgca
    13981 atacttgttg catccggtca gacaacttcc ctgttttatt tcccgcccgc caaatcgata
    14041 aaacttcccg tgacgtaaga ggctttatca cttagtagcc agacaatggc ctgcgcgacc
    14101 tcttctgcct gtccaccacg ctgcatgggg atgttcgact taacgcgatc gacgcgtcca
    14161 ggctcgccgc cgctggcgtg catttcggta taaataaacc ctggccgcac gcagttaacg
    14221 cggatcccct gcgcggcgac ttccagcgat cgtccggtgg ttaacgtatc aatcgcccct
    14281 ttcgatgccg cgtaatcaac atattcccct ggcgaaccca accgtgaggc caccgaagag
    14341 acattgacga tagcgccgcc actgccacca tttttaagcg ccatgcgttt taccgcctcg
    14401 cggcagcaga gaaaatatcc cgtcacgttg gtggaaagta ctcggttgat tcgctctgcg
    14461 gtaaggtttt caacggtgca ctgggtaaac aagatcccgg cgttattgac cagcgctgct
    14521 agcggttcat cgtgctgatc gattgctgta aacatcgcaa cgacctggtt ttcgtcgctg
    14581 atatccgcct ggagcacgaa tgctttgcca ccggcttgca ttattaagtt catcacttcc
    14641 tgcgccgcgt ggaggttttg ctgataatta accgccaccg tatacccttc ttgcgccaac
    14701 agtaatgcag ttgcccgccc gatgccgcga ctgccaccgg tcacaagtgc tatagccatt
    14761 tgtttttccg agaaaaatta attcgttgac gtatctttga gattgctaac aaactggctc
    14821 aatcttcaat gttggcacgc ttttccccct cgccctttca gggagatggt cggggtgagg
    14881 gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc ccaaaggggc
    14941 gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg ccgaagcgcc
    15001 tttagaaaat actcgaatca gtgaattact ggtattcgct aatcggtacg caggagcaga
    15061 acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag tatttgtctg
    15121 ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac tcggcgacca
    15181 gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc cagacaccgg
    15241 ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag cgatccagtt
    15301 ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc gggaacgaca
    15361 tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc tcgctgatgc
    15421 cgatttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg cgaccgtcgc
    15481 gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag ttggcgttga
    15541 ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg atgtacatcc
    15601 agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg ccctgacggg
    15661 ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc agatgcgctt
    15721 tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag aaagttttat
    15781 gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg ccaacctggg
    15841 cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg acttcacaca
    15901 cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc atgatacagg
    15961 agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg atgttgccgt
    16021 ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa gcggggttag
    16081 ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg cggctttcat
    16141 gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag ttcggctgca
    16201 tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc gcaatcatct
    16261 gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca aattccgtcc
    16321 aagtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa cccagcggga
    16381 tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag cgcatcattt
    16441 cggtttcgct gtgatagcga ttaaacaccg gatgggtgag gatttcgtcg tcgcgcagca
    16501 tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg tcgatctcca
    16561 gcccgtggtt atcgcccagc agcacactga aaagctgcat tacgttttca cgcgtggtgg
    16621 tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg atttcagccg
    16681 cttcggcacg cgtcagtacg cccgctttgt cggctacttc cacacacaag gtgtcgaaat
    16741 agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga atatcggtca
    16801 gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa acggcataca
    16861 ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc gctttctcac
    16921 gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg ccagctgcat
    16981 ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct ttcgccgcaa
    17041 agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc gccgaaccaa
    17101 aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc ataatatcgg
    17161 cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca gtgtagtcgt
    17221 gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc tgatggtcga
    17281 gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca cgagtacgga
    17341 ccacatccag tgtttgcgga tgcacattgg aagccacgaa gaagcggttg gcatttttca
    17401 gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc tcgtccagaa
    17461 gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg aagttgagca
    17521 gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg gtataccagc
    17581 ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg gtgtaaccca
    17641 tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt tctgccagtg
    17701 cggcgtattc ggtcgccggt gcgccaacct gcggtggtgt cgcaagctga atatctttcg
    17761 gcacaatctg gccggtcagc gcgtttaacg attgtgcacc aacggcattc agcatttctt
    17821 gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg ttttcaagct
    17881 ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt gtgaacctct
    17941 ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc ctctcccgca
    18001 gaagaggaat aaagccgtta ctcatcttct aacaatgctt cgtatgcggt cgcatccagc
    18061 agtgattcca gttcgctttc atcgctagct ttgattttaa agatccagcc gcctgcatac
    18121 ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc cacgatttca
    18181 ccgcttactg gcgcataaat gtctgacgcc gcttttaccg attcggcaac cgcgcagtca
    18241 tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat atcgcctaac
    18301 agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc tttacgcagc
    18361 cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa tcaatctcca
    18421 aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt gtcactttaa
    18481 ccggcatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct tccggcacgc
    18541 gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg ataatgcctt
    18601 catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc agcacgcctt
    18661 tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc acttccaggg
    18721 cttcacgacc gataaagtca cgatctgtcg gttcccaggc gatagtccag cccatgttgg
    18781 cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg cccgcttcca
    18841 gacgcagcgt gtcacgcgcc cccagaccgc agggcttaac gcccgcttcc accagcgcac
    18901 gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct tcaccggtat
    18961 aaccagtggt ggcaataaac agatcgccca cctgcacgcc aaagaacggt ttcatccctt
    19021 ccaccgcctg acgctgagca tcattaaaca gtgtggcagc ttttgcctga gcattcggcc
    19081 cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg aaaggttcag
    19141 cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca atgaggcgga
    19201 agaaatcttc agtaaagtag tagacgatga gatcatctat cacaccgcca gaggcattca
    19261 acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg ttcgccagca
    19321 gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc atatgcgaca
    19381 catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc gaaccgtaat
    19441 gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc gtgtgttgtt
    19501 cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc tgacaacttt
    19561 ttcatggtga aattatcacc acaaaaccca gcatcggagc cactcccggt ccccaacgca
    19621 atcgttctct tttgcctgaa cttaccaccg aaacagactg ttaaccataa ggtaaaattg
    19681 atcatcacat tagcttatgg ttaaaaaatg caaaaatcgc gacagaataa aaaaccaaaa
    19741 aatacaccaa tttctataca aagatgatgt gatgagaaag tcaatttgaa taagacaata
    19801 ttaagagcta aaaaaatgtc aaaaaacact aaatcaaaaa atcatggcat tagaaaatat
    19861 aatgcgaaaa cggaggtgaa attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa
    19921 accgggagat gaaagtgtga tgggtatcaa ataaacaaca gaggagaaat ttttaacgca
    19981 gccattcaga caaatcgttt aatcccattg cctggcggat aagttgcggc ttaacgccag
    20041 gaagcgtgtc ggccagtttc aaaccaatat cacgcagcag ttttttcgcc ggattagcgc
    20101 cggaaaagag atcgcgaaaa ccctgcatac cagccagcat cagcgccgcg ctgtgcttgc
    20161 ggctacgttc atagcggcgc aggtaaatgt actgcccgat gtctttaccc tgacgatgca
    20221 accgtttcag ttcagcaatc agctctgcgg cgtccataaa tccgagatta acgccctgcc
    20281 ccgccagtgg gtgaatagta tgcgcggcgt cgcccaccag cgccagacgg tgtgcggcaa
    20341 actggcgcgc ataacgcccc gtcagcggga acacctgacg cgcgctctca accttgcata
    20401 agccaaggcg attatcaaaa gcgatattta acgcgcgatt aaattcgtct tcacttgcct
    20461 gctgcatccg ctgcgcttcc tctggcgaca gtgaccagac aatcgagcaa agatgcggat
    20521 cgctaagcgg taaaaaggcc agaatgcctt cgccatggaa aacctgccgc gccaccgcat
    20581 catgcggttc ttccgtgcga atggtcgcta ccagcgcgtg atgctgataa tcccagaaag
    20641 tcagcggaat atctgctttg ttgcgcaacc aggaattagc accgtccgcg ccaatcacca
    20701 gacgcgccgt taacatgctg ccatctttca gcgtcaggaa ggtttcattt tctccccagg
    20761 cgacctgctg taattctgtg ggggccaaca gagtaatatc tgacgactgc tgcgctttgt
    20821 tccacagcgc gtagtgaatc actgagtttt caacgatgtg cccaagatgg ctatagccca
    20881 tgctttgatc gtcaaacgag atgtgaccaa aactgtcttt gtcccacact tccatgccgt
    20941 gatagcaact ggccctacga gcgagaatgt cctgccagac gccaagacgg gtgagtaatt
    21001 tttcgctggc ggcattgata gccgaaacgc gcagttgtgg tagtgcatct accgccagag
    21061 gttccggtac gcgctgctcc agtacggcaa cgcgtaagcc gctcccctgt aagccgcagg
    21121 caaccgccag ccccaccatg ccgccgccaa caatggctac atcaacactt tgcattgttt
    21181 attccttaaa accgccttca acgcgccacc caaccgaggg tgcgctgcgc cagcacatcg
    21241 cgtgccgggg tgaataattc catcgtcatc agtccgatgt tgcgcccgac aaccagcggt
    21301 gcccaacggt tggcaaaaag atgtaccagg ctgtccgtga cgccgatggt tgcttcgcga
    21361 tcgctctgtc gacgctgctg atagcggcac aatatgccgt aatcaccgat gtcttctccg
    21421 cgctcatgcg cctgagtcag ggtttccgca aggctcatga catctcgcat accgaggtta
    21481 aacccttgcc cggcaatcgg gtgcagagtt tgcgccgcat tgcccaccag cacagtgcga
    21541 tgggtaatcg gtctggcggc gcgggttaat gccagcggat aagcactgcg tttaccggcg
    21601 tgggtaattt gccccagtcg ccagccaaag gcagactgga gttcacggca aaacttctcg
    21661 tcgctccacg acagcacctc ttcgcgccgt tccagtggat gacaccagac cagcgaacag
    21721 cgtccgtcag acatcggcaa catcgccagc gggccatgtt gcgtgaagcg ttcaaaagcg
    21781 cggccttcgt gcgcaacaga agtagcaacg ttggcaatca ctgccagttg ttcgtaaggc
    21841 tcctgctgcc aatcaacgcc gcacgcggtg gctaacgctg aatgggtgcc atcagcggct
    21901 atcagcacgc ggcccgtcag catctcgccg ctctccagcg tcacttcaac gtgactctga
    21961 gtacgggcaa cgttagctac gcgatcgggg caatgcagca ttacgccagg tgcttttcgc
    22021 agcaatgcaa acagccgttg cccaacatta tgcaattcga caacctgccc cagcgccgcc
    22081 agttggtaat cttctgcggc gagggtgacg aatccagcat gaccacgatc gctgacatgc
    22141 acggtagtga tggcggttgc gcaatcagcc agagattgcc agacgccgat gcgcgccagt
    22201 tgctgacagg tacccgccgc cagcgctatc gctcgtccat caaagcccgg atgagcatgt
    22261 gactctggcg cagtcgcttc aatcaaatgt accggcagcg ccccgtgact taaccgggaa
    22321 atagccagcg ccagcgtcgc gcccgccatg ccgccaccga cgatgattac gctcattgct
    22381 ttctcgcagc agccatcaac gcttcgattt cttccggctt tttcaccacg ctggcggtga
    22441 ggttttcgtt gccggtttcg gtaatcacaa tgtcgtcttc aatacgaatg ccgataccgc
    22501 gatattgttc tggcacatcc gcatccggcg cgatatacag accaggctct acggtcagta
    22561 ccatccccgg ttccagaatg cgcgagcgat cctgaccata aacgccaacg tcatggacat
    22621 ccagtcctaa ccagtggcta aggccatgca taaagaaagg acgatgggcg ttctgagcga
    22681 tcagttcatc aacattacct ttcaggatgc cgagttttac caggccgcta accatgatgc
    22741 gcaccacttc accagtgact tccagaatgg aagttcccgg acgatacagg cgcaggctgg
    22801 tttcgagaga ctccagcaca atgtcgtaga tttcacgctg ggcctgggtg aatttgccgt
    22861 tgaccgggaa ggtgcgggta atatcgcccg cgtaaccttt gtattcacaa cccgcgtcaa
    22921 tcaacaccag gtcaccgtcg cgcagttcac tctcgttttc ggtgtagtgc agaatgcagc
    22981 cgttttcacc gctgcctaca atggtgttat aggaaggata gcgcgcaccg tggcggttaa
    23041 attcgtggtg aatttcgcct tccagatggt actcgaacat tcccggacgg catttttcca
    23101 tcgcccgtgt atgtgccatg gcggtgattt ctcccgcgcg gcggagtacg gcaatctctt
    23161 ctggcgattt gaacaggcgc atttcatgaa caacaggacg ccagtcgatc atcgttgccg
    23221 gtgcggtgag attttgccgc gaacctttac gcagtttttc cagcgcactg ttcacgattt
    23281 catcagcata tgcatattcg ccctgggcat ggtaaaccac atccaggccg ttaagtagtt
    23341 gataaagttg ctgattgatt tcgctgaatg ccagtgcgcg gtcaacgccc agtttctctg
    23401 gcgcggcatc ctggcctaag cgacggccaa accagatctc cgccgtcagg tcgcgaacgc
    23461 ggttaaacag aacgctgtgg ttatgagtgt catcgctttt aatcagcacc agcaccgctt
    23521 ccggttcgtt aaagccggtg aagtaccaga agtcactgtt ctgacgatag gggtattcgc
    23581 tgtcggcgct acgtgttact tctggtgcag caaaaatcag cgcggcgctg ccgggttgca
    23641 tttgctccac cagggcctga cggcgacgct gaaactcttg ccgggatatc tcactcataa
    23701 cactctcctt acgttttttg tttttagtgt agagtcggtt tttgtacttc tggcgcggtc
    23761 ggttgcggat gagtaaaggt gtcgtggcat aacagcgcgg caacacgaac gtattcgatg
    23821 atctcttcaa gcgacatttc aagctcttcc tgatcttcgt cttcgtcgta gcccagctgc
    23881 gcgatgttac gcagatcgtc gatggcttca ccggtttcgc cggtcacttt gtccagcttc
    23941 ggttgcgtaa cgccaagacc aagcaggaag tgattgaccc aacctgccag tgcatcagcc
    24001 cgatcgaaaa cgctgacatc atcgccatca ggcagataaa gctgaaaaag gaagccgtca
    24061 tcctgcaggg catcgctggt ggcagagtgc attttacgca gtgcctgtgc cagctcatga
    24121 ccgaaagcca tgccttcgtt cgtcaggtcg tgaagtagcg gcagccatga gctgtcatcg
    24181 ttaccgccac atatcatccc actgattaaa ccatgcatct cggcaggggt cagacccgtc
    24241 ccttgttggt tcagatactg gttcatttcg ttgtaaccag gcatttcgtt ctgtatagac
    24301 ataagcattc gtcgtcaaag ggaggaatat tcatgatatg ctaccacttt gggccctggt
    24361 ggaccagaaa agggcttgtc tcttctcatc agggtagcta tagcgtcgcc ccttcgcaga
    24421 ccatgggtct aaagacgaag gcagcgcagt caatcagcag gaaggtggca tgtctgcaca
    24481 acccgtcgat atccaaattt ttggccgttc actgcgtgtg aactgcccgc ctgaccaaag
    24541 ggatgcgttg aatcaggcag cggacgatct gaaccaacgg ttgcaagatc tgaaagaacg
    24601 cactagagtc acaaatactg aacagttggt cttcattgcc gcattgaata tcagctatga
    24661 gttagcgcaa gaaaaagcaa agactcgtga ctacgcggca agtatggaac agcgtattcg
    24721 gatgctgcag cagaccatag aacaagcgtt acttgaacaa ggtcgcatca ccgaaaaaac
    24781 taaccaaaac tttgaatgac acttttcggt ttactgtggt agagtaaccg tgaagacaaa
    24841 atttctctga gatgttcgca agcgggccag tcccctgagc cgatatttca taccacaaga
    24901 atgtggcgct ccgcggttgg tgagcatgct cggttcgtcc gagaagcctt aaaactgcga
    24961 cgacacattc accttgaacc aagggttcaa gggttacagc ctgcggcggc atctcggaga
    25021 ttcccttctt atctggcacc agccatgacg caactaccag agctcccact gacattatcc
    25081 cgacaagaaa tccgcaaaat gattcggcaa cgtcgtcgtg cgttaacgcc ggaacaacag
    25141 ctggaaatgg gtcaacaagc cgctacccgg atgatgactt atcccccggt ggtgatggcg
    25201 catacggtcg ctgtattcct ctcttttgat ggtgaactcg acacccagcc gctaatagaa
    25261 caactctggc gcgccggtaa gcgcgtatat cttccagttt tgcatccctt tagtgccggt
    25321 aatttgctgt tcctgaatta ccatccgcaa agcgaactgg tgatgaacag gttgaagatc
    25381 catgagccaa aactggatgt gcgtgacgtg ctaccccttt cccgattaga cgtgctgatc
    25441 acaccgctgg tcgcctttga tgagtacggt cagcgcctgg gaatgggcgg tggtttttat
    25501 gatcggacct tacaaaactg gcagcactat aaaacgcaac cggtgggtta tgcgcatgat
    25561 tgtcagttgg tggaaaaact ccccgttgaa gagtgggata tccctcttcc tgcggtggtt
    25621 acaccgtcga aagtctggga gtggtaaggg cgatacacct gcatcgccct gattgacatc
    25681 gttgattctt taacctaatt tagtgagtaa gggtaaggga ggattgctcc tcccctgtga
    25741 ctgttaataa gctgggaaac ttatgagtaa cagtacaatc agtatgatgg caagtcgcat
    25801 cataaccctt ctccttcaag ccctcgcttc ggtgagggct ttaccgttac agccccatgc
    25861 tgcccagcta tcgtaagctc tctttaaata accacaacgc attgatctac ctttaaatct
    25921 tttcttcgag cgtgctcgaa aaaccccatg taaaacagag ataatttttg ggaaaaagga
    25981 tctattacgg cagtaaacgt gggcgatgaa gtggctatca aaaagttcgg ttcagggcga
    26041 tgcaccccgc atcgccctga ttgacatcgt tgattctttg acctaattta gtgagtaagg
    26101 gtaagggagg atgactcctc ccttgagact gactgttaat aagctccgaa acttatcagt
    26161 aacagtacaa tcagtatgat gacaagtcgc atcataaccc ttctccttta agccctcgct
    26221 tcggtgaggg ctttaccgtt acagccccat gctgtcccgc catcgtaaat ccccattaaa
    26281 taaacacaac gcattgatct gactttgatt tattttctgg agcagactcg caaagtagaa
    26341 tgcgcaacgc ggcaacggtg tggagaaggg ataaaaaaac gggcaagtca gtgacctgcc
    26401 cgttaatttt cagagaaggg gaattagtac agcagacggg cgcgaatggt acccggaata
    26461 gctttcattg cctgcagcgc tttttcggca acgtcttcgt cagcttcaat atcaataact
    26521 acataaccca tctgggcgga agtttgtaga tattgcgcgg cgatgttgac gccctgctcg
    26581 gcgaagattt tgttcagcgc agttagcacg cccggacggt tttcgtggat gtgcatcaga
    26641 cgacgaccac catgcagtgg cagcgagact tccgggaagt tcaccgcaga gagcgttgag
    26701 ccattgtcag aatacttgat caatttaccc gcaacttcca agccgatatt ctcctgcgct
    26761 tcctgagtcg aaccgccaat gtgtggcgtc agaaggacgt tgtcgaattc acacagtgga
    26821 gaggtaaatg gatcgctatt ggtcgccggt tccgtcggga atacgtcgat tgccgccccc
    26881 gccagatgtt tgctcgccag cgcatcacac agcgccggaa tatccaccac agtaccgcgc
    26941 gaggcattaa tcagcagcga gccgggcttc attagcgaaa tctctttcgc gcccatcata
    27001 tttttggtgg acggattctc tggtacatgc agactcacca catcgctcat attcagcagg
    27061 tcagaaagat gctgtacctg agtggcgttg cccagcggca gtttgttttc aatatcataa
    27121 aagtaaacat acattcccag cgattcagcc agaatgccca attgcgtacc aatatgaccg
    27181 tagccgatga tacccagctt tttgccgcgc gcttcaaaag aacccgccgc cagtttgttc
    27241 cacacgccac ggtgcgcttt agcattggct tccggcacgc cgcgcaatag cagcagcagt
    27301 tcgccaatca ccagctccgc aacagagcgc gtatttgaga acggtgcgtt aaataccggg
    27361 atcccgcgct ttgccgccgc atccagatca acctggtttg ttccgataca gaaacagcca
    27421 atagcgacca gtttttctgc ggcgttgatc acgtcttcag tcagatgggt acgggatcgc
    27481 aggccgatga agtgggcatc gcggatggat tcttttaatt gttcatcatc cagcgcgcct
    27541 ttgtgaaatt cgatgttggt gtaaccagct gcacgaaggc tttccagcgc cttttggtgc
    27601 acgccttcta ccagcagaaa cttaatcttg tctttctcca gcgatacctt tgccatttac
    27661 ccaatcctgt cttttgaaat gttgtgtgcg gatttgcatc cgcctttcaa catatcaaaa
    27721 aataatattg cggcaatatg aacgtttgcg tcgcgatgtt gaggaaatat cacgcaaagg
    27781 taattcttgg atgaatatgc tggtttagag gatttattta gagcaatcga caattgcctg
    27841 gtaaaagcgt gacacatgtc accaaattta atgaagagaa tttttttaac gggggaggtt
    27901 cccccgtcag atcatttcac aatggttttg acaccgtcag gtgtgccaat cagcgcaacg
    27961 tccgcgccac ggttagcaaa caagccaaca gtcaccacgc caggaatcgc atttatggcg
    28021 ttttccatcg ctatcgggtc aaggatttcc atgccgtgga cgtcgaggat cacgttgcca
    28081 ttatcggtca ccacgccctg acggtattcc ggacgaccgc ccagtttcac cagctggcgc
    28141 gccactgcac tacgtgccat cgggataact tctactggca gcgggaattt acccagaata
    28201 tcaacctgct tggaggcgtc ggcaatacag ataaattttt ccgcaaccga agcaatgatt
    28261 ttttcacggg tcagcgccgc gccgccgcct ttgatcattt gcatgtggcc gttgatttca
    28321 tctgcgccat caacgtagat gccaaggctg tcgacttcgt tgagatcaaa aacgtgaatg
    28381 ccgaggcttt tcagtttttc agtggaagca tctgaactgg aaacggcccc ttcaatctgg
    28441 cctttcatcg tgccgagcgc gtcaataaag tgtgcggcgg tggaacctgt acctacacca
    28501 acaatggtac cgggctgaac atactgaagt gccgcccatc ctactgcttt tttcaattca
    28561 tcctgcgtca tgatcgtttc gcctgtggta tgaaatttca cacgcattat atacaaaaaa
    28621 agcgattcag accccgttgg caagccgggt ggttaactca tccataaaat atcgcgcaat
    28681 ggcgggcatc ccctttcgcc ccgcaaataa agcatacaac ggtctgggta tgccgctcca
    28741 cggtgcgaac agaggcaata acgccacgcc attcaacata accagggcgc gaagtcgcta
    28801 agcgaaatgc aatagcgcaa atccctgatc ttttaagctg cgcgtttaac cattgataca
    28861 aatgttaccc gctgacggct tcggtatatg caacctgaca caaaattgtg tcatagtgca
    28921 ggaaaaagca tttaccagga gcagacaaca gcaatgaaac gcccggacta cagaacatta
    28981 caggcactgg atgcggtgat acgtgaacga ggatttgagc gcgcggcaca aaagctgtgc
    29041 attacacaat cagccgtctc acagcgcatt aagcaactgg aaaatatgtt cgggcagccg
    29101 ctgttggtgc gtaccgtacc gccgcgcccg acggaacaag ggcaaaaact gctggcactg
    29161 ctgcgccagg tggagttgct ggaagaagag tggctgggcg atgaacaaac cggttcgact
    29221 ccgctgctgc tttcactggc ggtcaacgcc gacagtctgg cgacgtggtt gcttccggca
    29281 ctggctcctg tgttggctga ttcgcctatc cgcctcaact tgcaggtaga agatgaaacc
    29341 cgtactcagg aacgtctgcg ccgcggcgaa gtggtcggcg cggtgagtat tcaacatcag
    29401 gcgctgccga gttgtcttgt cgataaactc ggtgcgctcg actatctgtt cgtcagctca
    29461 aaaccgtttg ccgaaaaata ttttcctaac ggcgtaacgc gttcggcatt attgaaagcg
    29521 ccagtggtcg cgtttgacca tcttgacgat atgcaccagg cctttttgca gcaaaacttc
    29581 gatcttcctc ctggcagcgt accctgccat atcgttaatt cttcagaagc gttcgtacaa
    29641 cttgctcgcc agggcaccac ctgctgtatg atcccgcacc tgcaaatcga gaaagagctg
    29701 gccagcggtg aactgattga cttaacgcct gggctatttc aacgacggat gctctactgg
    29761 caccgctttg ctcctgaaag ccgcatgatg cgtaaagtca ctgatgcgtt actcgattat
    29821 ggtcacaaag tccttcgtca ggattaatcc atcaaataat gcctgatagc acatatcagg
    29881 cgttgtcctc acttcttttt gtattccttg aatcacatca caaaatagac aa
//
LOCUS       DADLTF010000036        28614 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.036, whole genome shotgun sequence.
ACCESSION   DADLTF010000036 DADLTF010000000
VERSION     DADLTF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.036"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..166
                     /locus_tag="JN749_001518"
     CDS             <1..166
                     /locus_tag="JN749_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="ethanolamine utilization protein; involved in the
                     degredation of ethanolamine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine utilization protein EutK"
                     /protein_id="HAY7367978.1"
                     /translation="EPDAPVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARN
                     ALEQLFSAGT"
     gene            251..1375
                     /gene="eutR"
                     /locus_tag="JN749_001519"
     CDS             251..1375
                     /gene="eutR"
                     /locus_tag="JN749_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HAY7367979.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITCQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWGGGSYTQSLTLINSLNAASGLPSFG"
     gene            complement(1309..2208)
                     /gene="hemF"
                     /locus_tag="JN749_001520"
     CDS             complement(1309..2208)
                     /gene="hemF"
                     /locus_tag="JN749_001520"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HAY7367980.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(2212..3081)
                     /gene="amiA"
                     /locus_tag="JN749_001521"
     CDS             complement(2212..3081)
                     /gene="amiA"
                     /locus_tag="JN749_001521"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HAY7367981.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            3295..3720
                     /locus_tag="JN749_001522"
     CDS             3295..3720
                     /locus_tag="JN749_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HAY7367982.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            3707..4156
                     /locus_tag="JN749_001523"
     CDS             3707..4156
                     /locus_tag="JN749_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HAY7367983.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            4217..4792
                     /locus_tag="JN749_001524"
     CDS             4217..4792
                     /locus_tag="JN749_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HAY7367984.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            4888..5787
                     /gene="yfeX"
                     /locus_tag="JN749_001525"
     CDS             4888..5787
                     /gene="yfeX"
                     /locus_tag="JN749_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HAY7367985.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(5967..7391)
                     /gene="murP"
                     /locus_tag="JN749_001526"
     CDS             complement(5967..7391)
                     /gene="murP"
                     /locus_tag="JN749_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HAY7367986.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(7395..8291)
                     /gene="murQ"
                     /locus_tag="JN749_001527"
     CDS             complement(7395..8291)
                     /gene="murQ"
                     /locus_tag="JN749_001527"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAY7367987.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            8254..9255
                     /gene="murR"
                     /locus_tag="JN749_001528"
     CDS             8254..9255
                     /gene="murR"
                     /locus_tag="JN749_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HAY7367988.1"
                     /translation="MFEPSVIIFSSCIGGDLTIYDQIIADVWNHISRIDRLKGIIEST
                     LAECEERHKNLSGIFAASPGEWKMLYLTKIRNAESEFTGNEQKIADFLRARVSELKSV
                     SSRQMAKQLGISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSIT
                     SDDSLEVIARKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGR
                     DLSFKLMKIGYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQG
                     ATVIAITSLADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            9442..10233
                     /gene="ucpA"
                     /locus_tag="JN749_001529"
     CDS             9442..10233
                     /gene="ucpA"
                     /locus_tag="JN749_001529"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HAY7367989.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            10537..11553
                     /locus_tag="JN749_001530"
     CDS             10537..11553
                     /locus_tag="JN749_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7367990.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            11553..12386
                     /gene="cysT"
                     /locus_tag="JN749_001531"
     CDS             11553..12386
                     /gene="cysT"
                     /locus_tag="JN749_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HAY7367991.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            12386..13261
                     /gene="cysW"
                     /locus_tag="JN749_001532"
     CDS             12386..13261
                     /gene="cysW"
                     /locus_tag="JN749_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HAY7367992.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            13251..14348
                     /gene="cysA"
                     /locus_tag="JN749_001533"
     CDS             13251..14348
                     /gene="cysA"
                     /locus_tag="JN749_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HAY7367993.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            14482..15393
                     /gene="cysM"
                     /locus_tag="JN749_001534"
     CDS             14482..15393
                     /gene="cysM"
                     /locus_tag="JN749_001534"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HAY7367994.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MHAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(15597..16320)
                     /locus_tag="JN749_001535"
                     /pseudo
     CDS             complement(15597..16320)
                     /locus_tag="JN749_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(16353..16727)
                     /locus_tag="JN749_001536"
     CDS             complement(16353..16727)
                     /locus_tag="JN749_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HAY7367995.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            16832..17683
                     /gene="pdxK"
                     /locus_tag="JN749_001537"
                     /gene_synonym="thiJ"
     CDS             16832..17683
                     /gene="pdxK"
                     /locus_tag="JN749_001537"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HAY7367996.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(17725..18234)
                     /gene="crr"
                     /locus_tag="JN749_001538"
     CDS             complement(17725..18234)
                     /gene="crr"
                     /locus_tag="JN749_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAY7367997.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(18275..20002)
                     /gene="ptsI"
                     /locus_tag="JN749_001539"
     CDS             complement(18275..20002)
                     /gene="ptsI"
                     /locus_tag="JN749_001539"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HAY7367998.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(20047..20304)
                     /gene="ptsH"
                     /locus_tag="JN749_001540"
     CDS             complement(20047..20304)
                     /gene="ptsH"
                     /locus_tag="JN749_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HAY7367999.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(20688..21659)
                     /gene="cysK"
                     /locus_tag="JN749_001541"
     CDS             complement(20688..21659)
                     /gene="cysK"
                     /locus_tag="JN749_001541"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAY7368000.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(21844..22605)
                     /gene="cysZ"
                     /locus_tag="JN749_001542"
     CDS             complement(21844..22605)
                     /gene="cysZ"
                     /locus_tag="JN749_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HAY7368001.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            22835..23722
                     /gene="zipA"
                     /locus_tag="JN749_001543"
     CDS             22835..23722
                     /gene="zipA"
                     /locus_tag="JN749_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HAY7368002.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRSMMT
                     PQKLREYQDIIREVKDANA"
     gene            23793..25808
                     /gene="ligA"
                     /locus_tag="JN749_001544"
     CDS             23793..25808
                     /gene="ligA"
                     /locus_tag="JN749_001544"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAY7368003.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            25810..26028
                     /locus_tag="JN749_001545"
     CDS             25810..26028
                     /locus_tag="JN749_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368004.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(26025..27023)
                     /locus_tag="JN749_001546"
     CDS             complement(26025..27023)
                     /locus_tag="JN749_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HAY7368005.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            27113..28039
                     /locus_tag="JN749_001547"
     CDS             27113..28039
                     /locus_tag="JN749_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7368006.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(28030..28371)
                     /locus_tag="JN749_001548"
     CDS             complement(28030..28371)
                     /locus_tag="JN749_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flxA-like family protein"
                     /protein_id="HAY7368007.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(28491..28613)
                     /locus_tag="JN749_001549"
     ncRNA           complement(28491..28613)
                     /ncRNA_class="other"
                     /locus_tag="JN749_001549"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         6894 a         7096 c         7713 g         6911 t
ORIGIN      
        1 ggaacccgac gcgccagtta tcgtcgcgga atctgctgac gagttgttgg cgctgttaac
       61 atcagtatgt cagggaatga cggcaggaga agtggccgcc cactttggct ggccgctgga
      121 aaaagccaga aatgcgctcg aacagctctt ttctgccggg acgtaacgta aacgcagtag
      181 tcgttatcgt ctcaagcccc attaacctgt cggaggtgcc gggtgtccta caacacccgg
      241 cattaacatc atgaaaaaga cccgtacagc caatttgcac catctttatc atgaaccctt
      301 acccgaaaac ctgaagctca cgccgaaggt cgaagtggat aatgttcatc aacgacagac
      361 aacggatgtc tatgaacatg ctttgacgat taccgcctgg cagcagattt acgatcagct
      421 gcatccgggc aagtttcatg gtgaatttac ggaaattcta ctcgatgata ttcaggtttt
      481 tcgtgaatac accggtctgg cgctgcgtca gtcgtgcctg gtctggccga actcgttctg
      541 gtttggcatt ccggcgacgc gcggtgagca gggatttatc ggttcgcaat gtctgggaag
      601 cgcagaaata gcaacgcgcc ccggtggaac cgagtttgaa ttaagtacgc cggacgatta
      661 tacgatcctg ggcgtagtgc tgtctgaaga tgtcatcacc tgtcaggcta actttttgca
      721 caatccggat cgggtattac atatgctgcg tagccagtcg gcgctggaag tgaaagagca
      781 gcataaagcc gcgctgtggg gctttgtcca acaggcgctg gcgacgtttt gcgagaaccc
      841 ggaaaatctc catcagcctg cagtgcgaaa agtgctgggg gataatttgc taatggcgat
      901 gggggcgatg ctggaagaag cgcaaccaat ggtgacggcg gaaagcatca gtcatcagag
      961 ttaccgtcgg ttgctttccc gcgcccgtga atatgtgctg gaaaacatgt ccgaaccggt
     1021 gacggtgctg gatttgtgta atcaactgca tgtcagtcgc cgcacgctac aaaacgcgtt
     1081 tcacgctatt ttaggcattg gcccaaacgc gtggctgaaa cgcattcgcc tgaacgccgt
     1141 acgccgcgaa ctgataagcc cgtggtcgca aagcacaacg gtaaaagacg ccgccatgca
     1201 gtggggattc tggcatctgg ggcaatttgc cacggattac cagcagctgt ttgccgagaa
     1261 gccgtcgttg acgttgcatc agcggatgcg ggaatggggg ggagggagtt acacccaatc
     1321 cctgacctta ataaactcac ttaacgccgc ttctgggctg ccatcttttg gctgataatc
     1381 atactcccag cgtaccagtg gcggcattga catcaggatc gactcggtgc gcccgccagt
     1441 ttgcaggcca aacagcgtgc cgcgatccca gaccagattg aactcgacat aacgaccgcg
     1501 acggtagagc tgaaaattgc gctcgcgctc gccgtaggcc atcgctttac gtcgctctac
     1561 aattggtaaa taagcgtcgg tgtagccttt gcctaccgcc tgcataaagg caaaacagtg
     1621 gtcgaaatct ggcgtgttca gatcatcaaa gaacagcccg ccaatgccgc gctgttcgtt
     1681 gcgatgtttg aggtagaagt aatcgtcgca ccactttttg taacggggat aaacgtcttc
     1741 accaaatggc tggcacaggt cacgggcggt gcggtgccag tgaatggcgt cttcttcaaa
     1801 accatagaaa ggggttaaat cgaagccgcc gccaaaccac caaacgggct ctgcacccgg
     1861 tttttcggca ataaaaaaac gcacattcgc gtggctggtg ggaacatacg ggttatgcgg
     1921 atgcactacc agtgaaacgc ccatcgcctc gaaactgcgc ccggcaagtt ccgggcgatg
     1981 agcggtggcg gaagcaggca tcgcctcacc gtggacgtga gaaaagttga cgcccgcctg
     2041 ttcgaaaaca ccaccattac gcaacacccg actgcgcccg ccgccgccag cttcgcgctg
     2101 ccaactatct tcgacaaatt ctgcgccatc gacggcggtc agctgctgac aaatcgtatc
     2161 ctgaaggttg agcagaaact gtttaacctg gtgtgcgtcg ggtttcataa cttatcgctt
     2221 tttcgaatgt gctttctggt tgtcgaacca gtggaaataa ctgatcacgc cttcagcaat
     2281 cgctgtggcg attttctgac gaaacgccgc cgtgcctaac agccgctctt cttccgggtt
     2341 ggtgataaac gaggtttcca ccagcaccga aggaaccgac ggcgatttca acaccacaaa
     2401 cgcggcttgt tcggtgttgc ggctgtgcag tttatgcacc ggcttaatct ttttcagaat
     2461 atgcgagcca agcgtcaggc tgtttttaat agtatcggtt tgcaccagat caaacagtac
     2521 ttgctgcaat aggtgatcct tgtcagtcgc ctttttaccg gcaacttcat cggcgcggtt
     2581 ttcgcgttca gacaggtatt tcgccattgc gctacttgcc ccacggttag agagggcaaa
     2641 taccgaagca ccggcagctt tcgggttggt aaagccatcg gcatgaattg acataaacag
     2701 atctgcgcca tgtttatggg cgatttcaac gcgatcgtaa agtgggataa acgtatcgcc
     2761 agaacgcgtt aaacgcgcat caatcccatg attacgcaaa atggaacgga cgtttttagc
     2821 aatcgccagc accacatgtt tttctttcga accgttgcgt ccgatcgctc cggtatcaat
     2881 tccgccgtgg cctggatcga gaacaacgac acgtttgccg ccagattttt ttgctttcgg
     2941 cttgctgtgt ccgttgctgg tttttaaagg ttcgtctttg gcaatggctt gcgacattcc
     3001 tgacaacgtc agggcagcta aaccggcttt cagcacctgg cggcgcgaag tgagtgtttt
     3061 tagtggttta aaagtgctca tacggcctga gttgtaataa taaagttcca gatgttatat
     3121 cgtatcgcgt aatccgttac gacggtttgc tgttgagaat tgttttactt ttcatttcaa
     3181 tagttgacaa ctccccatta tgccactttt tcaaccgatt ttcgtcagat attggctaat
     3241 tcgaccgttc acgccataat cagcctttta acccgcaaaa aggtaacgaa taccatggag
     3301 atacgcgtat ttcgccagga agatttcgaa gaggtcatca ccctttggga gcgttgcgac
     3361 ttgctgcgtc cgtggaacga tccggaaatg gacatcgagc gtaagatgaa ccatgacgtc
     3421 agtttgtttt tggtcgctga ggtaaacggt gaagtggtcg gaacggtgat gggcggttat
     3481 gacgggcatc gcgggtctgc ttattatctt ggcgtgcatc cagagtttcg tgggcgtgga
     3541 attgccaatg cgttgcttaa ccggctggag aaaaagctga ttgctcgcgg ctgcccgaaa
     3601 attcagatca acgtgccgga agataacgac atggtgctcg gaatgtatga acgcctggga
     3661 tatgaacacg ccgacgtgct gagtctgggt aagcgtttga ttgaagatga agagtactga
     3721 gttccatcct gtccattatg atgcgcacgg acgcctgcgt ttacccttgc tcttctggct
     3781 tgtgctattg cttcaggcgc gaacctgggt gctgtttgtc attgccggtg cgtcgcgcga
     3841 gcagggtact gcgctgctaa atctgtttta tcccgatcac gataatttct ggctggggct
     3901 gattcccggt attccggcgg tgctggcgtt tttgttgagc ggtcggcggg cttcgtttcc
     3961 tcgcacctgg catgtgctct attttctgtt gctgttggcg caggtggttt tactttgctg
     4021 gcaaccgtgg ctgtggctga acggtgaatc cgttagcggt atcggtctgg cgctggtagt
     4081 ggcggatatt gtggcgttaa tctggctgct gaccaatcga cgtttacgcg cttgttttaa
     4141 tgaagaaaaa gaataacggc actttttggt gaatttgcac tccaagcaac gttattgaat
     4201 aaccaaagga agtgacatga aatcgctgcg tttaatgtta tgcgctatgc cgttgatgct
     4261 gaccggctgt tccacgatgt cgtcagttaa ctggtctgcc gctaacccgt ggaactggtt
     4321 tggatcgtcc accaaagtga gcgagcaggg cgtgggtgaa ttaacggcgt ccacaccact
     4381 gcaagaacaa gccattgctg atgcgcttga tggcgattat cgcctgcgca gcggaatgaa
     4441 aaccgcgaac ggcaacgtgg tgcgcttttt tgaagtgatg aaaggcgaca acgtggcgat
     4501 ggtgattaac ggcgatcaag gcacgatcag tcgcattgac gtgctggata gcgatattcc
     4561 tgctgacacc ggtgttaaaa tcggtacacc gtttagcgac ctttacagca aagcatttgg
     4621 caattgccaa aaagctgatg gtgatgataa tcgtgccgtc gaatgtaaag ccgaaggcag
     4681 tcaacatatt agctaccagt tcagcgggga atggcgtggt cctgaagggt taatgccttc
     4741 ggacgatacc ctgaaaaact ggaaagtcag taaaattatc tggcggcgtt aatttgcgtc
     4801 tgaacaaacc gccgtcgcga aaaaacacgt aaaatagcgc tcaacaatgc cacggattgc
     4861 gtggcattct tattttcagg aggaacaatg tctcaggttc agagtggtat tttgccagaa
     4921 cattgccgcg cggcgatttg gatcgaagcc aacgtgaaag gggaagttga cgccctgcgt
     4981 gcggccagta aaacatttgc cgacaaactg gcaacttttg aagcgaaatt cccggacgcg
     5041 catcttggtg cggtggttgc ctttggtaac aacacctggc gcgctctgag cggcggcgtt
     5101 ggggcagaag agctgaaaga ttttccgggc tacggtaaag gccttgcgcc gacgacccag
     5161 ttcgatgtgt tgatccacat tctttctctg cgtcacgacg taaacttctc tgtcgctcag
     5221 gcggcgatgg aagcctttgg tgactgcatt gaagtgaaag aagagatcca cggcttccgt
     5281 tgggtggaag agcgtgacct gagcggcttt gttgacggta cagaaaaccc ggcgggtgaa
     5341 gagacgcgcc gcgaagtggc ggttatcaaa gacggcgtgg atgccggcgg cagctatgtg
     5401 tttgtgcagc gttgggagca caacctgaag cagctcaacc ggatgagcgt tcacgatcag
     5461 gagatgatga tcgggcgcac caaagaggcc aacgaagaga ttgacggcga cgaacgtccg
     5521 gaaacctctc acctcacccg cgttgatctg aaagaagatg gcaaagggct gaagattgtt
     5581 cgccagagcc tgccgtacgg caccgccagt ggcactcacg gtctgtactt ctgcgcctac
     5641 tgcgcgcgtc tgcataacat tgagcagcaa ctgctgagca tgtttggcga taccgatggc
     5701 aaacgtgatg cgatgttgcg tttcaccaaa ccggtaaccg gcggctatta tttcgcaccg
     5761 tcgctggaca agttgatggt gctgtaagcg tttctgattc attgattaaa ggccaggcag
     5821 taacattact gactggcctt tttattactg ctttaacgcc gcatatactt gatcaactac
     5881 cttctgtcag aacagggtat tttgcggcgc tgacgctaca actaacagcc agcagagaaa
     5941 gatagagcat tgtccgtttc ataatatcag tccagattga cgttacggca gccaaagagc
     6001 gtggtgaaaa taaacccgca aacccatgcc accagaatcc cgccagcata aaccgccatt
     6061 gccggaagga tgccttgtgc ggaagtcatc agcggcagcg ccaccagacc agacggccca
     6121 aaggcgctgt ttaagcccat cggcagaccc caccaggcta tcaatccgat aaacaaaccg
     6181 cccgccgcgc cgcctaaaca ggcggtaaca aacggcttca tgcggggcag ggtgacaccg
     6241 taaatcagcg gttcaccaac gcccagcagg ccgggaataa tcgccccgcg tacctgactg
     6301 cgtagcgcac tgtgcggttg cgcccgccag tagagtgcca gcgccgcgcc cacctggccc
     6361 gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc cctggctgtc cattaacgcg
     6421 aggtaaacag gaataaagcc ctgatgcacg ccaaatacca cggcgatcag gaacagcccg
     6481 gctaaaaccg cacaaccgaa cggattactg ttcaggtgca taaacagcca tgacatacct
     6541 tcgaataacc agccacccag cggcataatg atcaggtacg caagcgtagc ggtgatcagc
     6601 agggtgatta acgaggtcag cagcatgtcg agatcgtccg gcataaagcg gcgcaccatg
     6661 ccttcaatgc gggcgcaggc cctcgcggca atcagcacgc cgataatatt gccgcgcgga
     6721 tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt aaccggtggt tgcagcgggg
     6781 ttataaccga gcaaaaagag tgcggcaata attgcgccat tgacgcccgt gccgccaaat
     6841 gcctgggcgg cgttatagcc caccagaatc actaggaagg tgaacaaacc tttgctgaac
     6901 accttcataa aattcagcgc gtcgggtagc gtgccctgag catctgccgg aacgtgcatc
     6961 accgtggcaa ttaacgtcgc tatccccagc agcagaccgg cggcaataaa accggggatc
     7021 agcggcgtga agatggtggc gaatttggcg agaaattgtt gcacgccgga ggtttgtttg
     7081 gcttttaact gacgtttgtt ctgggcggcg atttctgcgg catcctgtac cggtgcctcg
     7141 cccagcagct cgctcatcgc ttttgcggca cgatgcgctt taccaggccc aaaaacgacc
     7201 tgcacctggt cgctggtcaa aatgacgccc ttcacgcctt ccagcgtttt gatattggga
     7261 tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg tcatacagtt accacaactg
     7321 gcgatatttc ccggtccgcc gacacgggta agaatggtgt tcagaagttc actgctgatc
     7381 tctttggcca tgggttattc cttgtctaaa acctgacgaa taaagccgcc gtgttgatcc
     7441 aggcgttttt tagcttctgc ggcatcgaga tttttcagca ccatcacaat ggccgtttta
     7501 cagttgcgct cgcaggcaat taacgccgct tccgcttgtt cggcattaca tccggtggcg
     7561 tttttaacga tattgacctg gcgcacatgc agtttttcgt tggtggcgac cacatcgacc
     7621 atcaggttgc cgaacacttt gccggattta atcatcagcc cggtggaaag catattgagc
     7681 accagtttct gcgctgtgcc tgctttcatc cgcgaagaac cggtaacaac ttcggcacct
     7741 acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg cgctccccgg attacaggaa
     7801 attcccactg tgcggcagcc gagctggcgt gcgtattcca gtccggcaat cacatacggc
     7861 gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg ccgttaaatt aatatttttc
     7921 agatcgttaa caccaccttc ccggctatct tcagcgcctt ccaccgcgtg ctgaatggca
     7981 tattcgcctc cagcaatcaa accaaccacc agacccggtt tcacgccgta ggtgggcgga
     8041 cattcgctgg catccagaat ccccagacga ccggatgttc ccgcaccgag ataaatcaga
     8101 cgaccgccgc cgctgacctg ggcgtggata acatcgatcg ccgcggcgat atccggcagg
     8161 acgcgatcaa cggcaagcgg tacggtttta tcttcatcgt tgataatccg gcacatttcc
     8221 agcgtcgata cgcggtcaat ttcagccgag gcggtgttcg agccttcagt aatcatcttt
     8281 tctagttgca tcggtggtga ccttactatt tacgatcaaa taattgcaga tgtatggaat
     8341 catatatccc gaattgacag acttaaagga atcattgagt ctacactcgc agagtgtgaa
     8401 gagcgtcata agaacttaag cggaattttt gctgccagcc caggagagtg gaagatgttg
     8461 tacctgacga aaatacgcaa tgcggaaagt gaatttaccg ggaacgagca aaaaattgct
     8521 gactttctgc gggccagagt gagcgagctg aaatccgtct cttcacggca gatggcgaaa
     8581 cagctcggta tcagccagtc gagcattgtg aaatttgccc agaagctcgg cgcgcaagga
     8641 tttactgaat tgcgtatggc gttaattggc gaatacagtg ccagccggga gaaaacgaat
     8701 gccacggcgc tgcacctgca cagttcgatt accagtgatg attcgctgga agtgattgcc
     8761 cgcaaactga atcgcgaaaa agagctggcg ctggagcaaa cctgtgcgtt gttcgactac
     8821 gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac cgtttatcca gataacgggc
     8881 ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca aactgatgaa aattggctat
     8941 cgcgtggcct gcgaagccga cacgcacgta caagccaccg tttcccaggc gttgaaaaaa
     9001 ggcgatgtac aaatcgctat ctcttacagc ggcagtaaaa aagagattgt cttgtgcgca
     9061 gaagcggcac gaaagcaggg ggcaacggtg attgccatta cttctctggc tgactctccg
     9121 ctgcggcggc tggcgcattt cacgctcgat accgtttccg gtgaaaccga atggcgtagc
     9181 tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg atttactgtt tgttggtctt
     9241 gtgcaacatc aatgatgtgg cgtcattgaa aatgattcag cgcagcagtg agctaacaca
     9301 acgcctgaaa tgatgcataa agcagcgact ggattgagat tttcctgaat tagtgagctg
     9361 atccgcagca atattttgtt tatcctgtat tttcagaggg aatggagtgt aacgctctgt
     9421 attaacaagg agagcattaa aatgggtaaa ctcacgggca agacagcact gattacgggc
     9481 gcattgcagg gaattggcga aggaattgcc agaacttttg cacgtcatgg cgcgaaccta
     9541 atcttgctgg atatctcccc tgagatcgaa aagctagcgg acgaactgtg tggtcgtggt
     9601 catcgctgta cggcggttgt cgccgatgtg cgtgacccgg cgtcggtagc cgcagctatc
     9661 aaacgcgcga aggaaaaaga agggcgcatt gatatcctag tgaataacgc aggcgtttgt
     9721 cgtctgggca gtttcctcga tatgagcgat gaagatcgcg atttccatat tgatatcaat
     9781 attaaaggcg tatggaacgt cacgaaggcg gtgctgccgg agatgattgc gcgcaaagat
     9841 ggtcgcattg tgatgatgtc ttcagtcact ggtgatatgg tggccgatcc tggcgaaacg
     9901 gcgtatgcct taacgaaagc ggcgattgtt ggcctgacta aatcgctggc ggtggagtac
     9961 gcgcaatccg gtattcgcgt taacgccatc tgcccgggat acgtccgcac gccaatggcg
    10021 gaaagcattg cccgccagtc gaacccggaa gatccagaat cggtgctgac tgaaatggca
    10081 aaagcaatcc cgctgtgtcg cctcgccgat ccgctggaag tcggcgaact ggcggccttc
    10141 ctcgcatcgg atgaatccag ctatttaacc ggtacacaga atgtgattga tggcggcagc
    10201 acactgccgg agacggttag cgtcggtatc tgattcacct ctgtttcctc cctgcatgtg
    10261 tggggaggat ttcgtcttga actaagttca ccaggctatt tcatttgtca ttttggcccc
    10321 gggcagtgct cgaaatcctc acgtactatg tgtacgctcc ggtttctccg cgctgtccgt
    10381 gtccaaactg cctgcaacaa ttacgcctgt tgaaccaagt tcttattccc tttttaactt
    10441 ccaaatcacc aaacggtata taaaaccgtt actcctttca cgtccgttat aaatatgatg
    10501 gctattagaa agtcattaaa tttataaggg tgcgcaatgg ccgttaactt actgaaaaag
    10561 aactcactcg cgctggtcgc ttctctgctg ctggcgggcc atgtacaggc aacggaactg
    10621 ctgaacagtt cttatgacgt ctcccgcgag ctgtttgccg ccctgaatcc gccgtttgaa
    10681 cagcaatggg caaaagataa cggcggcgac aaactgacga taaaacaatc tcatgccggg
    10741 tcatcaaaac aggcgctggc gattttgcag ggcttaaaag cagacgttgt cacttataac
    10801 caggtgaccg acgtacaaat cctgcacgac aaaggcaagc tgatcccggc tgactggcag
    10861 tcgcgcctgc cgaataatag ctcgccgttc tactccacca tgggcttcct ggtgcgtaag
    10921 ggcaacccga agaatatcca cgactggaac gacctggtgc gctccgacgt gaagctgatt
    10981 ttcccgaacc cgaaaacgtc gggtaacgcg cgttatacct atctggcggc atggggcgca
    11041 gcggataaag ctgacggtgg cgatgaagcc aaaaccgaac agtttatgac ccagttcctg
    11101 aaaaacgttg aagtgttcga taccggcggt cgtggcgcaa ccaccacttt tgccgagcgt
    11161 ggcttgggcg atgtgctgat tagctttgaa tcggaagtga acaacatccg taaacagtat
    11221 gaagcacagg gctttgaagt ggtgatcccg aaaaccaata ttctggcgga attcccggtg
    11281 gcgtgggtcg ataaaaatgt gcaggccaac ggtacggaaa aagccgcaaa agcctacctg
    11341 aactggctct acagcccaca ggcgcagacc atcatcaccg actattacta ccgtgtgaat
    11401 aacccggaag tcatggacaa actgaaagat aaattcccgc agaccgaact gttccgcgtg
    11461 gaagacaaat ttggctcatg gccggaagtg atgaaaaccc acttcaccag cggcggcgag
    11521 ttagacaagc tgttagcggc ggggcgtaag taatgtttgc agtttcttca agacgcgtgc
    11581 tgccgggctt tactttaagc cttggcacca gtctgctgtt tgtgtgcctg atattgctgc
    11641 tgccgctctc tgcgctggtg atgcagcttt ctgagatgag ctgggcgcag tactgggagg
    11701 tgatcaccaa cccgcaggtg gtcgcggcct acaaagtgac gctgctgtcg gcgtttgtgg
    11761 catcgatttt taacggcgtt ttcggtctgc tgatggcgtg gattttaaca cgttatcgct
    11821 ttccgggccg cacgctgctt gatgcgctga tggatttacc ctttgcgctg ccaacggcag
    11881 tcgccggttt aacgctggcc tcgctctttt ccgtaaacgg tttttacggt gaatggctgg
    11941 cgaagtttga tatcaaagtc acctatacct ggcttggtat tgcggtggcg atggccttta
    12001 ccagcattcc gtttgtggtg cgtaccgtgc agccggtgct ggaagagtta ggcccggaat
    12061 atgaagaagc ggcggaaacg cttggtgcaa cgcgctggca gagtttctgc aaagtggtgc
    12121 tgccggagct ttctccggcg ctggtggcgg gcgtggcgct gtcgtttacc cgtagtcttg
    12181 gtgaatttgg cgcggtgatt tttatcgccg gaaatatcgc gtggaagacg gaagtgacgt
    12241 cgctgatgat ttttgtgcgc ttacaggagt ttgattaccc ggcagcgagc gcgattgctt
    12301 cggtgatcct cgcggcatct ctgctgctgc tgttctcaat taacactctg caaagtcgct
    12361 ttggtcggcg tgtggtaggt cattaatggc ggaagttacc caattgaagc gttatgacgc
    12421 gcgcccgatt aactggggca aatggtttct gattggcatc gggatgctgg tttcggcgtt
    12481 catcctgctg gtgccgatga tttacatctt cgtgcaggca ttcagcaagg ggctgatgcc
    12541 ggttttacag aatctggccg atccggacat gctgcacgcc atctggctga cggtgatgat
    12601 cgcgctgatt gccgtaccgg taaacctggt gttcggcatt ctgctggcct ggctggtgac
    12661 gcgctttaac ttcccgggac gccagttact gctgacgcta ctggacattc cgtttgccgt
    12721 atcgccggtg gttgccggtc tggtgtatct gctgttctac ggctccaacg gcccgctcgg
    12781 cggctggctg gatgaacata atctgcaaat tatgttctcc tggcccggaa tggtgctggt
    12841 caccatcttt gtgacgtgtc cgtttgtggt gcgcgagctg gtgccggtga tgttaagcca
    12901 gggcagccag gaagacgaag cggcgatttt gcttggcgcg tccggctggc agatgttccg
    12961 tcgcgtcaca ttgccgaata ttcgctgggc gctgctttac ggcgtggtgc tgaccaacgc
    13021 ccgcgcgatt ggcgagtttg gtgctgtgtc ggtggtttcc ggctcgattc gcggcgaaac
    13081 actttcgctg ccgttacaga ttgaattgtt ggagcaggac tacaacaccg tcggctcctt
    13141 taccgccgcc gcgctgttga cgctgatggc gattatcacc ctgtttttaa agagtatgtt
    13201 gcagtggcgc ctggagaatc aggaaaaacg cgcgcagcag gaggagcatc atgagcattg
    13261 agattgccaa tatcaagaag tcttttggtc gcacccaggt gctgaacgat atctcactgg
    13321 atattccttc tggtcagatg gtcgcgttgc tggggccgtc cggttccggg aaaaccacgc
    13381 tgctgcggat tatcgccggg ctggagcatc aaaccagcgg gcatattcgc ttccacggca
    13441 ccgacgtgag tcgcctgcac gcgcgcgatc gtaaagtcgg tttcgtgttc cagcattacg
    13501 cgctgttccg ccatatgacg gtattcgaca atatcacttt tggcctgacg gtgctgccgc
    13561 gtcgcgagcg cccgaatgcc gcagccatca aagcgaaagt gacaaaattg ctggaaatgg
    13621 tccagcttgc gcatctggcg gatcgttatc cggcgcagct ttccggcggc cagaaacagc
    13681 gtgtggcgct ggcgcgcgcg ctggctgtag aaccgcaaat tctgctgctt gatgaaccgt
    13741 ttggcgcgct ggatgcgcag gtgcgtaaag agctgcgtcg ctggctgcgt caactgcatg
    13801 aagagctgaa attcaccagc gtgtttgtga cccacgacca ggaagaggcg accgaagtag
    13861 ccgatcgtgt agtggtgatg agtcagggga atattgaaca ggctgatgcg ccgaatcagg
    13921 tatggcgcga accagcgacc cgttttgtac tcgaatttat gggcgaagtg aaccgcctgc
    13981 agggaaccat tcgcggcggg cagttccatg ttggcgcgca tcgctggccg ctgggctaca
    14041 cacctgcgta tcagggtccg gtggatctct tcctgcgccc gtgggaagtg gatatcagcc
    14101 gccgtaccag tctcgattcg ccgctgccgg tacaggtact ggaagccagc ccgaaaggtc
    14161 actacaccca attagtggtg cagccgctgg ggtggtacaa cgaaccgctg acggtcgtga
    14221 tgcatggcga cgatgccccg cagcgtggcg agcgtttatt cgttggtctg caacatgcgc
    14281 ggctgtataa cggcgacgag cgtattgaaa cccgcgatga ggaacttgct ctcgcacaaa
    14341 gcgcctgata ggttgagtga atgttaaacg cccggaggcg cttcccgcga tccgggcttt
    14401 ttaatggcaa ggtttgtaac ctgtaggcct gataagacgc gcaagcgtcg catcaggcaa
    14461 caccacgtat ggatagagat cgtgagtaca ttagaacaaa caataggcaa tacgcctctg
    14521 gtgaagttgc agcgaatggg gccggataac ggcagtgaag tgtggttaaa actggaaggc
    14581 aataacccgg caggttcggt gaaagatcgt gcggcacttt cgatgatcgt cgaggcggaa
    14641 aagcgcgggg aaattaaacc gggtgatgtc ttaatcgaag ccaccagtgg taacaccggc
    14701 attgcgctgg caatgattgc cgcgctgaaa ggctatcgca tgaaattgct gatgcccgac
    14761 aacatgagcc aggaacgccg tgcggcgatg catgcttatg gtgcggaact gattcttgtc
    14821 accaaagagc agggcatgga aggtgcgcgc gatctggcgc tggagatggc gaatcgtggc
    14881 gaaggaaagc tgctcgatca gttcaataat cccgataacc cttatgcgca ttacaccacc
    14941 accgggccgg aaatctggca gcaaaccggc gggcgcatca ctcattttgt ctccagcatg
    15001 gggacgaccg gcactatcac cggcgtttca cgctttatgc gcgaacaatc caaaccggtg
    15061 accattgttg gcctgcaacc ggaagaaggc agcagtattc cgggcattcg ccgctggcct
    15121 gcggaatatc tgccggggat tttcaacgct gcgctggtgg atgaggtgct ggatattcat
    15181 cagcgcgatg cggaaaacac catgcgcgaa ctggcggtgc gggaaggaat attctgtggc
    15241 gtcagctcag gcggcgcggt tgctggagca ctgcgggtgg caaaagctaa ccctggtgcg
    15301 gtggtggtag cgatcatctg cgatcgtggc gatcgctacc tctccaccgg agtgtttggg
    15361 gaagagcatt ttagtcaggg ggcagggatt taaggattaa tagcattgga gactgatgac
    15421 aaacgcaaaa ctgcctgatg cgctccgctt atcaggccta caatgtttct gcaatatatt
    15481 gaattagcaa tatttttatg ccggatcagg cgtttacgcc gcatccggca taaacaaagt
    15541 gcactttgtc aataatctga tttgtcggca gttgttgctg ccgacaaatg tagtttttaa
    15601 ttttcgtgtg gaaaagaagt aaggtcatca atcatttttt gaagtattgg tgagtcctga
    15661 ccgtcacccc attgaagaat ttttgcggta agctgatgac gcgctagtgc attgagtccc
    15721 atctttttaa gtttatggga gattttaccc atgactttga tctgaccgaa tgcagtcgcg
    15781 taggtaatca tattgctctg gatagagctc atttcatcgg ctagtaattg cgcacgcaca
    15841 tcctcttcaa gaatactgtc catagcgatg tatttcatac cccacatagt ttcaattaac
    15901 atttgaggga aatcagcaca gtccagaacg gaggctattt tcaagttcgt ttagagcatc
    15961 agcaccttca tcgctaccaa aaggagattc ttcatcccag caacacatat aaagtggatc
    16021 agtaaaatgg cactggaagt ttgggtgtga cgttttaaga tgtaacccaa tctcttcatc
    16081 atcaaaatag tagagatcca tgaagttaaa gtctggagtt tcttgatatc cttttttcat
    16141 ttttgcggca atcaatttac tggcttcttt cagacactgt tcttcattat cgaattcttt
    16201 tgtatggaat ttaccaatac tacctacctt gccatagttg acagctaaac tatccccttc
    16261 gtaatctatc caccaaaatt tattcgattt ttcatcgtga taaataaagc attttttcat
    16321 gttacctcct gtaaatgcct taccaaagcg gattaatttc cttccgcagg caccgtttta
    16381 tccgtctgcg caattaacgc atgtaaaaaa ggctgtgcgt tctcatcgct cttaccgggg
    16441 atcttcacga tatacggctt cccggtaatc gatgacgacg aagctacctt atcaataaac
    16501 tgctcggcag tgtcaatgcg gttacgggta ttgccgagct tcagacgcag gtgggaaacc
    16561 gcttcatcgc aggtatgttc atcaccgttg cgcacaaata tcaaatcctt tttctgtgct
    16621 aatccctcca gcatggcgtt gatgcgggct tcttcatgaa cagttaactt cgcgtaagcg
    16681 gggagtgtca ttagcagcgt aatggccaga caaatgattt tcttcactga attaccttat
    16741 ctttgttttg taatagccga tttgactgcc agaatcaggt aaagtttcgg ttggaatacg
    16801 atgaggatca tcggcaagga gaaaaaatta aatgagtagt ttgttgttgt ttaacgataa
    16861 gagtagggcg ctgcaggcgg atatcgtcgc cgtgcagtcg caggtagtgt acggaagcgt
    16921 aggcaacagc attgcggtgc ctgctataaa acagaacggc ttgaatgtct ttgccgtgcc
    16981 gacggtattg ctgagcaata cgccgcatta tgacactttc tacggtggtg cgattccgga
    17041 cgaatggttt agcggctatt tgcgtgcgct tcaggagcgt gatgcgctgc gccaacttcg
    17101 tgctgtaacc acgggctata tgggaacggc atcgcaaatc aaaatccttg ccgagtggct
    17161 gactgcgcta cgcaaagacc atcctgacct attgatcatg gtcgatccgg tgattggcga
    17221 tattgatagc ggaatttatg tcaaacctga ccttcccgag gcgtatcgac aatatttact
    17281 gccgctggcg cagggaatta cccccaatat ctttgagctg gaaatcctga ccggtaaaaa
    17341 ttgccgcgat ctcgacagtg ccattgctgc cgcaaaaagt ctgctttcag acacattaaa
    17401 atgggtggtg attaccagcg cctccggtaa tgaagaaaat caggagatgc aggtggtggt
    17461 ggtcagtgcc gacagcgtga atgtcatttc ccattcacgg gtaaaaaccg acctgaaagg
    17521 gactggcgac ctgttttgtg ctcagcttat cagtggtttg ctgaaaggga aggcgttaaa
    17581 cgatgcggtg caccgtgcgg ggttgcgcgt actggaagtg atgcgctaca cccagcagca
    17641 tgagagcgat gaattgattt tgccgccgct ggcggaagca taaaaaatgg cgccgatggg
    17701 cgccattttt cactgcggca agaattactt cttgatgcgg ataaccgggg tttcacccac
    17761 ggttacgcta ccggacagtt tgatcagttc tttgatttcg tccatgttgg agataacaac
    17821 cggagtcagg gtagacttgg ctttctcttc cagcagcggc agatcaaatt caatgacagt
    17881 atcgccaact ttcacgcgct gaccttcttc agcaatacgc ttgaagcctt cgcctttcag
    17941 ttcaacggtg tcgataccga agtggacgaa cagttcaacg ccgctatcag attcgataga
    18001 gaacgcgtga ttggtttcaa agattttacc aatggtaccg tctacaggcg cgaccatttt
    18061 gttacccgtt ggtttgatag caataccatc accaacgatt ttttccgcaa aaacgacatc
    18121 cggcacgtct tcgatattga cgatctcgcc agagagcgga gcaatgatct caatagttcc
    18181 ggtatccttt ttgtcgtcgg aaaccagaga tttcagttta tcgaacaaac ccatgatctt
    18241 ctcctaagca gtaaattggg ccgcatctcg tggattagca gattgttttt tcttcaatga
    18301 acttgttaac cagcgtcatt aactcgtccg ttgtcggttg agcaagagcc tgctctgcta
    18361 acaccttcgc atcttcgaag ttcgtgttac ggataatctt cttaatgcgc gggatagaaa
    18421 tggcgctcat agagaattcg tccagaccca tccccagcaa cagaagtgta gcacgttcat
    18481 cgccagcaag ctcaccacac atgccagtcc atttgccttc agcatgagaa gcatcaataa
    18541 cttgcttgat caagttcagc acggacggtg acattggctg gtaaaggtgt gaaatcatat
    18601 cattaccacg gtcaactgcc agagtgtact gcgttaaatc attggtgccg atactaaaga
    18661 aatcaacttc tttggctaaa tgacgtgcaa ttgttgcggc agccggtgtt tccaccatta
    18721 cgccgatttc aattgactcg tcaaacgctt taccttcgtc gcgcagttcc tgtttgtaga
    18781 tttcgatctc tttgcgcagt gcacgcactt cttcaacaga gatgatcatc gggaacataa
    18841 tgcgcaattt accgaaagcc gaggcacgca ggatagcgcg gagctgatcg cgcaggatct
    18901 ctttacgatc catcgcgata cggatagcgc gccagccgag gaacgggttc tcttctttcg
    18961 ggaagttcat gtatggcagc tctttgtcgc cgccgatgtc catggtacga acgataaccg
    19021 cctgcgagcc acacgcttca gccactgctt tgtaagcagc aaactgttct tcttcagtgg
    19081 gcagcgcgtc gcggtccatg aacaggaact cagtacgata cagaccaacg ccttcagcgc
    19141 cgttacgctc tgcaccttca acgtcacgaa ccgtaccaat gttagcgcat acttctacct
    19201 ggtgaccgtc cagcgtaata gctggcagat ctttcagttt agcaagctct gctttttcag
    19261 aagccacttg ctcctgaaca gcgcgcattt tatcaataac ttcgttggtt ggattgacgt
    19321 aaacctgatt atttacggca tccagaatca gatagtcgtc atttttcacc tgagaggtga
    19381 cgctaccggt acccacgata gcaggcagtt ccagagaacg cgccatgata gaggtgtggg
    19441 aagtacggcc acccgcgtcg gtgatgaaac ccagcacctt cttcaggttc agctgtgcgg
    19501 tttcggacgg cgtcaggtca gcggcaacca gaatgacttc atcctgaatg gcgctcaggt
    19561 cgataatctt caggcccagg atgttgcgca gcaggcgctt accgatatca cgtacgtcag
    19621 ccgcacgttc tttcaggtat tcatcatcca gctcttccag ggcagaagcc tgaccttcga
    19681 taacttcatg agcagctgcg tcagctgtca tgtgtttatc tttaatcagg gctatgattt
    19741 cctgctccag ctcctcatct tcgagcagca taatatgccc ttcaaagatg gcttcttttt
    19801 cttcaccgaa cgtttcacca gctttcgttt tgatcgtttc cagctgggct gatgccttgg
    19861 cacgaccgct cagaaaacgt tcaacttcct gagcaacctg gtcggcagaa atttttttcc
    19921 ggtcaatgac aatttcgtct tctttcagaa gcagagcttt accgaaagcg atacccgggg
    19981 atgctaaaat gcctgaaatc ataaccctac cttacttgtg actgattttt aaaagaaccc
    20041 gggaaattac tcgagttccg ccatcagttt aaccagatgt tcaaccgctt tctgctcgtc
    20101 ttcgccttct gcggagatag tcacaacggt accttgagtc aggcccagag tctgcagttt
    20161 aaacaggctt ttcgcgctgg cgcttttgcc gttggaagtc acagtaattt cagaagtgaa
    20221 gcccttagct tcttttacaa actgggcagc agggcgggtg tgcagaccgt tcggagcggt
    20281 aatggtaact tcttgctgga acattgtatt tccccaactt ataggtttag tgttgtggaa
    20341 ctaaagtcta gcctggcggt tcgactttag cctgtattgt tagcgccagt ttttaacaga
    20401 cgcgacgcac gaagagcgca atacagtcca ttgctggcgg gtgttgaccg ctgacgtttc
    20461 gtacgtcatt aaacattatg ccgctaaagg aagaattgaa ccaaatcata aaatcgattc
    20521 agctttggct tttcctgtaa cgattaattt cgcgcatcaa aataattagt ctggttaaat
    20581 accagttccg acaggctgaa tcaatgccag gaggggcgaa agtttgaagc aggccacaaa
    20641 aaagcaccta aaaaggtgct tttttacgca tttttaacaa gctggcatta ctgttgcaat
    20701 tctttctcag tgaagagatc ggcaaacaat gcggtgctta aataacgctc acccgatgat
    20761 ggtagaataa ccacaatatt cttgttggta aagctttcat cttcttgtag tttcaacgct
    20821 gcggcaacag ctgctccaga agagatacct gcaagaatac cttcttcttc cattagacga
    20881 cgggcggtag aaatggcttc ttcattggtg atgccaatga ctttatcgac cagcttgaga
    20941 tcgaggttag ccgggataaa accagcgcca ataccctgaa ttttatgcgg gccaggttta
    21001 atctcttcac ctgccagcac ctgggcgata actggagaat cggttggctc aacggcgaca
    21061 gagataagat cggtcttgcc tttggtgcct ttaatgtagc ggctgacgcc agtcagcgta
    21121 ccgccagtcc caacgccagc aataaataca tcaacctgac cgtcggtatc ttcccagatc
    21181 tccggaccgg tggttttttc gtgaatttca gggtttgccg gattgctgaa ttgttgcagc
    21241 agcaggtatt tctctggatt gctggcgaca atttcttctg ctttttggat tgcgcctttc
    21301 atgcctttag caccttccgt cagcaccagg tttgcaccta acgctttcag cagcttgcgg
    21361 cgttcaatac tcatggtttc tggcatggtc agggtgagtt tgtaaccgcg agcggcagct
    21421 acataggcca gtgcaatccc ggtattaccg ctggtcggtt caaccagttc aacgcctggt
    21481 ttcagcacgc cgcgcttttc ggcatcccaa atcatgttgg caccgatacg gcacttaacg
    21541 ctgaagctgg ggttacgaga ttccaccttc gccagaatgc gtccgttacc gatgcgattc
    21601 aggcgaacca gcggcgtgtg accgatagtc agcgagttat cttcaaaaat cttactcatg
    21661 gcctgtcctt aactgtatga aattgggata caacaggtag catacccgct cagagaatat
    21721 gcggaagtaa ggatttagca tatctatata cagaagggaa ataatgacat gcaagatgga
    21781 ataaggggcg gcataagcca cccctgtttc acacaaacgg tttacaaaat aaccggtaga
    21841 ttgttaccgc cacatcgcgt gtttatcgcg atagcaatcg acccacatcg ccgtcgcgcc
    21901 acaaacggca acgggcatga tgaacagatt aagcagcggg atcatcgtaa acaggctggt
    21961 taaagcacca aactgcatat tggtgatttt gcgtgtgcgc agggcggtgc gcatctcttt
    22021 aaacggcact ttgtggttat cgaaggggta atcgcaatac tggatggcta acatccaggc
    22081 gctaaacagg aaccacagta ccggcgcgac ggtttgccca ataccaggga tgaagtaaag
    22141 aattagtaat acaattgcgc gcggcagata ccagacaaat ttttgccatt cgcgtttcat
    22201 gattcgcggc acatctttca tgataccgaa aatcccggta tctggcggtg tagcgccagt
    22261 caatcgtgct tccagttgtt cagccaataa accgttaaac ggagcggcaa tccagttagc
    22321 aatcgtggag aagaaatagc caaacactaa cagcacagag atgacagcca gaggccacaa
    22381 cagataactc agccattgta gccagtccgg aacgtaactc atgaaagtcg ggatccagac
    22441 atcgagctgt gtaaagagcc accagaatgc gccccccatc aacaaaatat tgaccagcag
    22501 cggtaaaata acgaaacgcc gaatcccagg ttgcgagacg agcttccagc cttgcgcaaa
    22561 atagtaaaaa ccgctgcgtg gggcagatgt gaatgatgaa accataatca ggatgagctc
    22621 cttttgacca atcccaggaa aattctgcgt attttaccgg gtaattgcgc aatggacagt
    22681 taggatatgt tcgaaaaaac agcaaaaagc acgatttcat ctatctttgt gctgtgaaag
    22741 ttaatagtgc acttgcactt gaggtaatcg gcaaatactc ttagtgagta aatgtttgcc
    22801 gtggtggcaa ggtgttagaa caacagagaa tataatgatg caggatttgc gtctgatatt
    22861 aatcattgtt ggcgcgatcg ccataatcgc tttactggta catggtttct ggaccagccg
    22921 taaagaacga tcttctatgt tccgcgatcg gccattaaaa cgaatgaagt caaaacgtga
    22981 cgacgattct tatgacgagg atgtcgaaga tgatgagggc gttggtgagg ttcgtgttca
    23041 ccgcgtgaat catgccccgg ctaacgctca ggagcatgag gctgctcgtc cgtcgccgca
    23101 acaccagtac caaccgcctt atgcgtctgc gcagccgcgt caaccggtcc agcagccagt
    23161 ttcgccacag gtcgcgccag cgccgcagcc agtgcattca gcaccgcaac cggcacaaca
    23221 ggctttccag cctgcagaac ccgtagcggc accacagcct gagcctgtag cggaaccggc
    23281 tccagttatg gataaaccga agcgcaaaga agcggtgatt atcatgaacg tcgcggcgca
    23341 tcacggtagc gagctaaacg gtgaactgct tcttaacagc attcaacaag cgggcttcat
    23401 ttttggcgat atgaatattt accatcgtca tcttagcccg gatggcagcg gcccggcgtt
    23461 attcagcctg gcgaatatgg tgaaaccggg aacctttgat cctgaaatga aggatttcac
    23521 tactccgggt gtcactatct ttatgcaggt accgtcttac ggtgacgagc tgcagaactt
    23581 caagctgatg ctgcaatctg cgcagcatat tgccgatgaa gtgggcggtg tcgtgcttga
    23641 cgatcagcgc agtatgatga ctccgcagaa attgcgcgag taccaggaca tcatccgcga
    23701 agtcaaagac gccaacgcct gatacactta aggcaaatta actcctcttc gaacccccgc
    23761 ttgtcggggg tttttagcat tgatggtgcg atatggaatc aatcgaacaa caactgacag
    23821 aactgcgaac gacgctttgc catcatgaat atctttatca tgtgatggat gcgccggaaa
    23881 ttcccgacgc tgaatacgac aggctgatgc gcgaactgcg cgagctggaa accaaacatc
    23941 cagaactgat tacgcctgat tcgcctaccc aacgtgtagg tgctgcgccg ctggcagctt
    24001 tcagccagat acgccatgaa gtaccaatgc tgtcactgga taacgttttt gatgaagaaa
    24061 gctttcttgc tttcaacaaa cgtgtgcagg accgtctgaa aaacaacgag aaagtcacct
    24121 ggtgctgtga gctgaagctg gatggccttg ccgtcagtat tctgtatgaa aatggcgttt
    24181 tagtcagtgc cgcgacccgt ggcgatggca ccaccgggga agatatcacg tctaatgtgc
    24241 gtactattcg cgccattccg ctgaagctgc acggagagaa tatcccggcg cgtctggaag
    24301 tgcgtggtga agtgttcctg ccgcaggcgg ggttcgaaaa gattaacgaa gatgcgcgac
    24361 gcacgggcgg gaaagttttt gctaacccac gtaatgcggc agctggttca ctgcgtcagc
    24421 ttgatccgcg tattacagcg aagcgaccgc tcactttttt ctgctatggc gttggtgttc
    24481 tggaaggggg cgagctgccg gatactcatc ttggccgttt actgcaattt aaaaagtggg
    24541 ggttgccggt cagcgatcgg gtaacgcttt gtgaatcggc ggaagaagtg ctggcgttct
    24601 atcacaaagt ggaagaagac cgcccgacgc tgggctttga tatcgacggc gtggtgatta
    24661 aggtcaactc actggaacag caggagcagc ttggctttgt cgcgcgtgcc ccgcgctggg
    24721 cggtagcgtt taaattcccg gcgcaggagc agatgacctt tgtgcgtgac gtcgagtttc
    24781 aggttgggcg tactggcgcg attacgcctg ttgcgcgtct ggaacctgtc catgttgctg
    24841 gcgtgctggt gagtaacgca accttacaca atgcggatga aatcgaacgt cttggtttac
    24901 gcattggcga taaagtggtg attcgccgcg ctggcgacgt gatcccgcag gtggttaacg
    24961 tcgtgctttc tgaacgcccg gaagataccc gtgaggttgt attcccgacg cattgtccgg
    25021 tatgtggttc tgacgttgag cgtgtggaag gtgaagcggt tgcccgctgt accggtggcc
    25081 tgatttgcgg tgcgcagcgt aaagagtcgc tgaaacactt tgtttcccgc cgtgcgatgg
    25141 atgttgacgg aatgggcgac aaaatcatcg atcagctggt tgaaaaagaa tatgtccata
    25201 ctccggcaga tctgttcaaa ctcaccgcag gcaaactgac cggactggag cgtatggggc
    25261 caaaattggc acaaaacgtg gttaacgcgc tggaaaaagc gaaagaaacc acctttgctc
    25321 gcttcctcta tgcacttggc atccgtgaag tcggcgaggc caccgcagca ggtctggcgg
    25381 catatttcgg cacgctggaa gcgctggaag ccgcttcgat tgaagagctg caaaaggtgc
    25441 ctgatgttgg cattgtcgtt gcatcccacg ttcacaactt ctttgccgaa gaaagcaacc
    25501 gcaatgtcat cagcgagctg ttggcggaag gtgttcactg gcctgcgccg atcgttatca
    25561 acgcggaaga gattgacagc ccgtttgctg gtaaaaccgt ggtgcttacg ggcagcttaa
    25621 gccagatgtc gcgtgatgac gctaaagctc gactggtcga actgggcgcg aaagtcgcgg
    25681 gcagcgtgtc gaagaaaacc gatctggtga tagcgggtga agctgcagga tctaaactgg
    25741 cgaaggcgca ggaactgggc attgaagtca tcgacgaagc ggaaatgctg cgtttgctgg
    25801 gtagctgaga tggaaaaaga gcagctgatt gaaatagcca atacgataat gccgtttggc
    25861 aaatacaaag ggcgtcgctt aattgacctg ccggaggagt atctgctgtg gtttgcccgc
    25921 aaagatgagt tcccggcagg gaagctcggt gagctaatgc aaatcacgct gctgattaaa
    25981 accgaggggc tgacgcaact ggtccagccc ctgaagcgtc cgctttaagc tttatcggcg
    26041 ctgctttcct gctgcgcctg taactgttcg gtctggcgtt tgtatcgacg cgctagcacc
    26101 gcacagacca tcaattggat ctgatggaaa atcatcaggg gcagcaccat cataccgatc
    26161 accgatgtgg ggaacagaat gtttgccatc gggatgccat ttgccagact ctttttcgaa
    26221 ccacaaaaga cgatagtaat ttcatctgcc ttattgaagc ccagtcggcg tgccataaag
    26281 acgttaacta caatcacgat agccagaaga acgcagctga ccaccacgat aaacagcaat
    26341 gatccccagc caactttatg ccagatacca ttaacgacgg cttcgctgaa cgctgtataa
    26401 accaccaaca gaatggacgt ctggtcagtt ttcgcaatcc attttttatt gcgcgacacc
    26461 cagtcaccaa tccacggccg ggaaagatgc cccaacacaa acggcagcag cagttgcagc
    26521 ataattttac cgacctgctc aaggctgccc cttgcaccgt gaacgttcat caccagacca
    26581 accagcaatg gtgaaaggaa aatccccagc aggctggatg ctgacgcaga acaaaccgcc
    26641 gccgcgacgt taccgcccgc cattgacgtg aaggcgattg cagactgcac ggtagccggg
    26701 agaatgcaca agtagagaaa accggagtag agcatcgggt cgacatttac cggtttccac
    26761 caggcaaaca gtacacccag aatcggaaac agcacgaagg tgctgcacat tacccacaaa
    26821 tgcagtcgcc agtgaccacc gccagcaata atcgcctcac gcgacaactt cgcgccgtgc
    26881 ataaagaaca gcagggcaat agctgcggtg gtcagatttt caaagaaggg gacgaaatcg
    26941 cctctggccg gaaagaaaga ggccagcaac accaccgtga tcagggttaa ggtgaaagga
    27001 tcgaggatac gaaaaagttt cataaagact ccagatgatc gatggttcta ttgtgcttct
    27061 tccagtttga gaaataaaat tgatttattg catccattca tgaagaaaac agatgaatta
    27121 ttctttaaaa caattaaagg ttttcgtcac agtagcgcag gagaaaagtt ttagtcgtgc
    27181 aggagagcgt atcggcttga gccagtcggc agtaagtcac agtgtgaagg agctggaaaa
    27241 tcatactggt gttcgcctgc tggacagaac cacgcgtgaa gtggtgctta cagatgcagg
    27301 gcagcagttg gctttgcgtc ttgagcgact gctggatgaa cttaacagca cgttgcgcga
    27361 taccgggcgt atggggcaac aactgagcgg aaaagttcgg gtcgctgcca gccagaccat
    27421 ttccgcgcat cttattccgc aatgcattgc tgaaagccat cgccgctatc cagatattca
    27481 gtttgtcctg catgatcgtc cgcagcagtg ggtgatggaa agtattcgcc agggagatgt
    27541 cgactttggc atcgtcatcg atccaggccc agtgggcgat ctgcaatgtg aagcgattct
    27601 ttccgagcct ttctttctgc tttgccatcg cgatagcgct ttggccgtgg aagattatgt
    27661 accctggcag gcattacagg gagcgaaatt ggtgttgcag gattacgcgt caggcagccg
    27721 accgctgatt gacgcagcgc tggcgcgcaa tggtattcag gcgaatattg tgcaggagat
    27781 tggacatccg gcgacgctgt tcccgatggt agccgcaggc atcggcatca gtattcttcc
    27841 tgcactggcg ctaccattgc ctgaaggtag cccactggtg gttaagcgca ttacgccggt
    27901 ggttgaacgc cagttgatgc tggtgcgcag gaagaaccgt tcactttcca ccgccgctga
    27961 agcgttatgg gacgtggtac gagaccaggg caatgcgcta atggctggtc gggaagggga
    28021 tccgttgtat cagatataga tatcgatatg agtcgttttt tcaacaggat ttagaaggga
    28081 tacattcaga gacaaacgct gctctttctc ctgcgccttt tctgcctgct gtttctgcaa
    28141 ctgcgccagt tgtgtttcca gtagggtgat ttgctgttga atcaattcgg cctgtttttg
    28201 cttgtcttca gcactgccgc tagtgtctac aatctcttta atttgttgag tgagtttgat
    28261 gatttgctga cttatccgcg cgatttgtga agcaacatcg ctaccttgtg atgccctgtt
    28321 actactctgt gtttgtatgg aaggaattga agttgaagag atagtggcca ttgttcagtc
    28381 ctcgtcactg tacgaggtaa tatcggtaga agaacaagga acttgagaag aaaaatcaat
    28441 cttacaaatc gcagatagca aaaaagcgcc tttagggcgc ttttttgcat tggtgggtcg
    28501 tgcaggatga ctcgcttcgc tcgcccttcg ggccgtcgcc gcgagcggct ccgttgtttc
    28561 gctgcgctca actcaaacct gctgcaggtt cgaatctttg agtatcgcag atag
//
LOCUS       DADLTF010000037        28370 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.037, whole genome shotgun sequence.
ACCESSION   DADLTF010000037 DADLTF010000000
VERSION     DADLTF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.037"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="JN749_001550"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="JN749_001550"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HAY7368008.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JN749_001551"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="JN749_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HAY7368009.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="JN749_001552"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="JN749_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HAY7368010.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /locus_tag="JN749_001553"
     CDS             2778..4127
                     /locus_tag="JN749_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7368011.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="JN749_001554"
     CDS             4278..5927
                     /locus_tag="JN749_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HAY7368012.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="JN749_001555"
     CDS             complement(6081..7373)
                     /locus_tag="JN749_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAY7368013.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKENDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JN749_001556"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="JN749_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HAY7368014.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="JN749_001557"
     CDS             complement(9197..9511)
                     /locus_tag="JN749_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HAY7368015.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JN749_001558"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="JN749_001558"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HAY7368016.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="JN749_001559"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="JN749_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HAY7368017.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDKQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="JN749_001560"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="JN749_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HAY7368018.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="JN749_001561"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="JN749_001561"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HAY7368019.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="JN749_001562"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="JN749_001562"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HAY7368020.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="JN749_001563"
     CDS             15750..17468
                     /locus_tag="JN749_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HAY7368021.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSMLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="JN749_001564"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="JN749_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HAY7368022.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="JN749_001565"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="JN749_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HAY7368023.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="JN749_001566"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="JN749_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HAY7368024.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /locus_tag="JN749_001567"
     CDS             complement(20170..20859)
                     /locus_tag="JN749_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7368025.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JN749_001568"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="JN749_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JN749_001569"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="JN749_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HAY7368026.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JN749_001570"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="JN749_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27750)
                     /locus_tag="JN749_001571"
     CDS             complement(26812..27750)
                     /locus_tag="JN749_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HAY7368027.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADIALTKLHLEMTTVRAPFDGRIISLKTSVGQFASAMRPIFTLIDTRHWYVIA
                     NFRETDLKNIRSGTPATIRLMSDSGKTFEGKVDSIGYGVLPDDGGLVLGGLPKVSRSI
                     NWVRVAQRFPVKIMVDKPDPEMFRIGASAVANLEPQ"
     gene            complement(27769..28044)
                     /locus_tag="JN749_001572"
     CDS             complement(27769..28044)
                     /locus_tag="JN749_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368028.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28253..>28370)
                     /locus_tag="JN749_001573"
     CDS             complement(28253..>28370)
                     /locus_tag="JN749_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368029.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6472 a         7178 c         7615 g         7105 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcggtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttagtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctggagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag atcatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg ggggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcag ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tcattttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagccgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcaccaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgc gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac aagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg cgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggcgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagca tgctgtcgtg ggaactcaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctgttgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta gcagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagcagatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttctg cggcgcgctg cgccgttctt gcacggtcaa cgtcttctgc
    27301 tgagacaaaa ccctctttca gtaatggctc ggtgcggcgt aatgtatctg tggcctgttt
    27361 cgcggcggca cgggcttttt ctaccgtggc attaaccgag tcggcaccaa actgttgggc
    27421 gtcaacgcta cgctgggtga gcataatttg cttatccagc gccgcgaggg aggcttcggc
    27481 tttcgccagg ttggcttcgt acgggcgcgg gtcgatgcgg aacagcaaat cgccctgttt
    27541 caccgcctgg ttgtcggtga ccgccagttc tacaatgcga ccgctgactt ccggcaccac
    27601 atcaatggta tctgctgacg cgtaagcgtc attagttgat ggcgcactgt ctacgcgcca
    27661 gataacgaaa acaagggcaa ccagcgccaa cgcaaccact aacagagcag ggaatttact
    27721 gcgaggtgct tttttcggcg tactttccat gagcgcgtct caaatagatt agaaaaatgc
    27781 cagccagaac agcatggcgt agagagcaaa aagggcggta taaataattc cgacaaatgc
    27841 cagattgata tttgcccgct gaataactcg cctcgtgatg agcgtcaaaa taaggctggc
    27901 aatggcgcag aaaaaccagc tgggataata agcgccgatc accgggattg ccggagataa
    27961 tgagcatccc gaaaccatga agacgggaat tatccaggct gtttttttga ggtgtatcgc
    28021 catgcgagag agaactgttg gcatattaaa tacatcctgt ctgctgcgaa caatatctgt
    28081 agtgaatatc aatatccatt gaaggcgtca gtatggctat tttctcgatg aataaaaata
    28141 gctttatcca gccaccgcat tcataactta tatattttat ttattaattg gctgtatata
    28201 tttttaaaat aaatgctttt gggggcttca gcgcccccaa aagcgagtga cattaatttg
    28261 gggttacgat attcacccac aaatcgaaat tatccagaca ggcgataatt tcttccagtt
    28321 tcgcgccatt gccaataatt ttggctctct tcgctttcac cagatccgcc
//
LOCUS       DADLTF010000038        28025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.038, whole genome shotgun sequence.
ACCESSION   DADLTF010000038 DADLTF010000000
VERSION     DADLTF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.038"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(98..448)
                     /gene="ubiK"
                     /locus_tag="JN749_001574"
     CDS             complement(98..448)
                     /gene="ubiK"
                     /locus_tag="JN749_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HAY7368030.1"
                     /translation="MTYPYRTTMVLNTYQYRETTMIDPKKIEQIARQVHESMPKGIRE
                     FGEDVEKKIRQTLQAQLTRLDLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTE
                     IKKQPDPETLPPTL"
     regulatory      510..658
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            762..1415
                     /gene="ribB"
                     /locus_tag="JN749_001575"
     CDS             762..1415
                     /gene="ribB"
                     /locus_tag="JN749_001575"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HAY7368031.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     gene            1676..1846
                     /locus_tag="JN749_001576"
     CDS             1676..1846
                     /locus_tag="JN749_001576"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368032.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="JN749_001577"
     CDS             complement(1904..2677)
                     /gene="zupT"
                     /locus_tag="JN749_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HAY7368033.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            2793..3608
                     /gene="ygiD"
                     /locus_tag="JN749_001578"
     CDS             2793..3608
                     /gene="ygiD"
                     /locus_tag="JN749_001578"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HAY7368034.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLV
                     ELLAPIPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            complement(3646..4806)
                     /locus_tag="JN749_001579"
     CDS             complement(3646..4806)
                     /locus_tag="JN749_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7368035.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(4812..5483)
                     /locus_tag="JN749_001580"
     CDS             complement(4812..5483)
                     /locus_tag="JN749_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HAY7368036.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="JN749_001581"
     CDS             complement(5631..7112)
                     /gene="tolC"
                     /locus_tag="JN749_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HAY7368037.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQACLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7317..7946
                     /gene="nudF"
                     /locus_tag="JN749_001582"
     CDS             7317..7946
                     /gene="nudF"
                     /locus_tag="JN749_001582"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HAY7368038.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            7947..8369
                     /locus_tag="JN749_001583"
                     /pseudo
     CDS             7947..8369
                     /locus_tag="JN749_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
     gene            8394..9221
                     /gene="cpdA"
                     /locus_tag="JN749_001584"
     CDS             8394..9221
                     /gene="cpdA"
                     /locus_tag="JN749_001584"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HAY7368039.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            9221..9802
                     /gene="yqiA"
                     /locus_tag="JN749_001585"
     CDS             9221..9802
                     /gene="yqiA"
                     /locus_tag="JN749_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HAY7368040.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            9831..11723
                     /gene="parE"
                     /locus_tag="JN749_001586"
     CDS             9831..11723
                     /gene="parE"
                     /locus_tag="JN749_001586"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAY7368041.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(11771..12085)
                     /locus_tag="JN749_001587"
     CDS             complement(11771..12085)
                     /locus_tag="JN749_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7368042.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="JN749_001588"
     CDS             complement(12116..12697)
                     /gene="mdaB"
                     /locus_tag="JN749_001588"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HAY7368043.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            13016..13348
                     /locus_tag="JN749_001589"
     CDS             13016..13348
                     /locus_tag="JN749_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HAY7368044.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="JN749_001590"
     CDS             complement(13394..14743)
                     /gene="qseC"
                     /locus_tag="JN749_001590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HAY7368045.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="JN749_001591"
     CDS             complement(14740..15399)
                     /gene="qseB"
                     /locus_tag="JN749_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HAY7368046.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            15551..15943
                     /gene="ygiW"
                     /locus_tag="JN749_001592"
     CDS             15551..15943
                     /gene="ygiW"
                     /locus_tag="JN749_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HAY7368047.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            15996..16478
                     /locus_tag="JN749_001593"
     CDS             15996..16478
                     /locus_tag="JN749_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7368048.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            17024..19282
                     /gene="parC"
                     /locus_tag="JN749_001594"
     CDS             17024..19282
                     /gene="parC"
                     /locus_tag="JN749_001594"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAY7368049.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            19516..20253
                     /gene="plsC"
                     /locus_tag="JN749_001595"
                     /gene_synonym="parF"
     CDS             19516..20253
                     /gene="plsC"
                     /locus_tag="JN749_001595"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HAY7368050.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            20328..21740
                     /gene="ftsP"
                     /locus_tag="JN749_001596"
     CDS             20328..21740
                     /gene="ftsP"
                     /locus_tag="JN749_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HAY7368051.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            21851..23893
                     /locus_tag="JN749_001597"
     CDS             21851..23893
                     /locus_tag="JN749_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005071065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HAY7368052.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSPRLLSACANH"
     gene            23874..24068
                     /locus_tag="JN749_001598"
     CDS             23874..24068
                     /locus_tag="JN749_001598"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein"
                     /protein_id="HAY7368053.1"
                     /translation="MPAPTIEEMREARRQNRNTRPALTKHTPMATQRQTPATAKKASS
                     TQSRPVNAGAKKRPKAAVGR"
     gene            complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="JN749_001599"
     CDS             complement(24111..24368)
                     /gene="yqhH"
                     /locus_tag="JN749_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HAY7368054.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(24419..25345)
                     /locus_tag="JN749_001600"
     CDS             complement(24419..25345)
                     /locus_tag="JN749_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HAY7368055.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNNNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="JN749_001601"
     CDS             complement(25545..26372)
                     /gene="dkgA"
                     /locus_tag="JN749_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HAY7368056.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="JN749_001602"
     CDS             complement(26477..27640)
                     /gene="yqhD"
                     /locus_tag="JN749_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HAY7368057.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKAAR"
     gene            27834..>28025
                     /locus_tag="JN749_001603"
     CDS             27834..>28025
                     /locus_tag="JN749_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7368058.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
BASE COUNT         6567 a         6946 c         7443 g         7069 t
ORIGIN      
        1 gtgatgatac tctgaatggg attaagcgat aatgtaatag aaaacactgt aaattagagg
       61 attaattcat aatttacagt gcattaaaga caaatgttta cagcgttggg gggagagtct
      121 ctggatctgg ctgctttttg atctcagtac tgcgattttc cagttcgctg attctttgtt
      181 caagcagcgc cagtttctca cgagtccgta acaggacttg cgtttggacg tcgaactctt
      241 cacggcttac aaggtcgagg cgcgtcagct gagcttgtag ggtttggcgg atttttttct
      301 ccacatcttc cccgaactcc ctgattcctt taggcattga ttcgtgaacc tggcgagcga
      361 tttgctcaat ttttttcggg tcaatcattg tagtttccct gtactgatag gtgttgagta
      421 ccattgtagt gcgataaggg taagtcataa accagaatta tgtgaagcta tgcgttgctg
      481 ccgctaatca ttagagttat agtgaatccg cttattctca gggcggggcg aaattcccca
      541 ccggcggtaa atcaactcag ttgaaagccc gcgagcgctt tgggtgcgaa ctcaaaggac
      601 agcagatccg gtgtaattcc ggggccgacg gttagagtcc ggatgggaga gagtaacgat
      661 tctgtcgggc atggacccgc tcacgttatt ttggctatat gccgccactc ctaagactgc
      721 cctgattctg gtaaccataa ttttagtgag gtttttttac catgaatcag acgctacttt
      781 cctcttttgg tacgcctttc gaacgtgttg aaaatgcact ggctgcgctg cgtgaaggac
      841 gcggtgtaat ggtgcttgat gatgaagacc gtgaaaacga aggcgatatg atcttcccgg
      901 cagaaaccat gaccgttgag cagatggcgc tgaccattcg ccacggtagc ggtattgttt
      961 gcctgtgcat cactgaagat cgccgtaaac aacttgatct gccaatgatg gtagaaaata
     1021 acaccagcgc ctatggcacc ggttttaccg tgaccattga agcggctgaa ggtgtgacta
     1081 ccggtgtttc tgccgctgat cgtattacga ccgttcgcgc agcgattgcc gatggcgcaa
     1141 aaccttctga tctgaatcgc ccgggccacg ttttcccact tcgcgcgcag gcaggtggtg
     1201 tactgacgcg tggcggtcat actgaagcga ctattgattt gatgacgctg gcaggcttta
     1261 aaccggctgg tgtgctgtgt gagctgacta atgacgatgg cacgatggcg cgtgcaccag
     1321 agtgcattga gtttgccaat aaacacaata tggcgctcgt gactattgaa gacctggtgg
     1381 cataccgtca ggcacatgag cgtaaagcca gctgaaaacc gctgcttaat tactgcctta
     1441 atcaagaaac cgaagttgta gctggcttcg gtttttattt tgccctgcta tgcaagatgt
     1501 taattccgct tctctttcta tgagaaaatt tcattaatat caggcattct ttttcattat
     1561 gatcaaaatc atataattgt tccgttatta tgcgaccata tgaatatatc cgaaaaagga
     1621 tatgtgaaat ttatgcgcta aatgtttagt attgttaaat attaagggta aaaggatgtt
     1681 tatcgcctgg tactggattg tattgattgc tctggttgtg gtgggttatt tcctgcattt
     1741 gaaacgttat tgtcgggcgt ttcgccagga cagagacgca ctgcttgaag cgcggaacaa
     1801 atacttaaac agtacgagag aagagacagc cgaaaaggta gaataaggct ttcgtgaagc
     1861 tggcagaagt tgtccatgtg ctggacaact tctgatgttg tttttaacca attcccgccg
     1921 tttgtagcag cactaaactg aatcccatca ctgacattcc acatagtacg ccatagctgg
     1981 ggttattatt agggtcaatc tctttggcga gcggcattaa ttcatcaacc gagagcgcca
     2041 ccataattcc tgcaaccgcc gccatgattg ccgccatgac caccggggaa atcatgctac
     2101 cgaggattaa ccacgccagc acaccaccaa gaatttctgc tagtccagaa atccccgccc
     2161 acagaatcgc ggtgcgttta gaccccgttg ccgcataaac cgggcctgcc actgccagac
     2221 cttcagggat attgtgcaac gcgacggcca gtgcgatgcc aaatcccagc tccaggttgc
     2281 tgctcgccgt gacaaaggtg gcaatccctt ccgggaagtt atgcagactg atgccgagag
     2341 tgagcagaat ggctgtgcgc ttgatcgatt ttggcaacag ctgcaccgat ttttgcatta
     2401 aatcctgcgg atgcgcatgc ggcaacatgc ggtccaggcc aaagtagcca agcagaccga
     2461 agataaacat tccataaccc aacaccggcg acattccttc agctgctagt gcggcaggaa
     2521 gcatttccat taatgagatg agcaacatga tccccgccgc aaaacctagc gaaaacgcca
     2581 gtaagcggtt cgagggtttt tgtccgagaa cgccgagaaa cgcgccaata aacgtggctg
     2641 cccccgccag tatggtcaga atgagaggta ctgacatcga caactcctta taaatcttca
     2701 ccttcaggca gatcgtcaaa ataactgatg atcatcatca ttgttatccg ggttcttact
     2761 ccgccgaaca agcgtattgt gaggatatca aaatgacacc tttagttaag gatatcatca
     2821 tgtcttcaac acgtatgcca gcattgtttt taggtcacgg tagtccgatg aacgtgctgg
     2881 aagataattt gtatacccgc agctggcaga agttggggat gacattgcca cgcccgcaag
     2941 cgattgtggt ggtttcggct cactggttta cccgtggaac aggagtgacc gcgatggaga
     3001 cgccgcccac gattcatgac tttggtggct tcccgcaggc gctgtacgat acgcattatc
     3061 ctgctccggg ttcgcctgcg ctggcacagc gtctggttga gctgttagcg ccgatcccgg
     3121 tgacgctgga taaagaagcc tggggctttg accacggctc gtggggcgtg ctgattaaga
     3181 tgtatcctga cgccgatatc cctatggtgc agttgagtat cgacagtagc aaacctgccg
     3241 cctggcattt cgaaatgggg cgcaaactcg cggcgttgcg tgatgaagga ataatgttgg
     3301 tcgccagcgg taacgtggtg cataacctgc gcacagtgaa gtggcacggt gatagttcac
     3361 cgtatccgtg ggcgacgtcg tttaatgagt atgtgaaagc gaatctgacg tggcaagggc
     3421 cggtagagca gcatcctctg gtgaattatc tcgaccatga aggtggcgcg ttatcgaacc
     3481 caacgccaga gcactatctg ccgttgttgt atgtgttagg tgtgtgggat gggcaggagc
     3541 caattaccat tccggtcgat ggtatagaaa tgggcagttt aagtatgctg tcggtgcaga
     3601 taggctaaca attaccggat ggcgagtgcc atccggtatc gtggcttatt caacaaaaat
     3661 atgtggataa aaccgagaca tatcctgggt gatcaacgca cggtcttcac gaatgccaat
     3721 tccggcaggt tgatcgttca ccagccagct accaatcagc atatagctgt cgccgaattt
     3781 cggtaacggg tggaattgct gaacaatcat cccttcttcg ccatacggac cttccgctgc
     3841 ttcaatggtt ttgccgttct caatgatcga cacgtttgcg ccttcacggg agaagatcgg
     3901 tttaaccaca tatttttcca tttgcggatg atcatcttcc gcaaaataag cgggcagcag
     3961 gttcgggtga ttcgggaaca tctcccacag tagcggtaga agtgccttgt tggagataat
     4021 actcttccac gccggttcca gccagcgtac gccagcatct tccagcttgg tggagaacat
     4081 ttcgcgcagc ataaattccc acggataaag cttgaacagg ttagaaatca cctgatcctg
     4141 taaatccgtg aactggcctt tttcgcctaa acctatctcg tcgatgtaga ggaactcggt
     4201 ggcgatttca gcttcggttg cgcagtcctg caaatactga atggttccgc gatcttccac
     4261 cgtgtcgcga cagcaggtga gatgcagcaa ctggaagcca cactgttcac gcagctcaac
     4321 gaagcgatcg atcagttttt cttgcagact gttaaactgg tcgctgccct ccggcaagtt
     4381 accggcgtta agctgatctt ccagccagat ccactgaaag aacgccgcct cgtatagtga
     4441 cgttggcgta tcggcgttat tttccagaag tttaggttcg ccagtgccat cccacgccag
     4501 atcaagacgc gaataaagcg atggctggtg agtcagccat gattgacgca caaaactcca
     4561 ggtgtgtttt ggtatgcgga atttggtcat cagctcatcg ctggcgatca ctttttccac
     4621 cactttcagg cacatctggt gcagttcggc ggtgacttct tccagctttt caacctgggc
     4681 gagggtcaac ttgtagtaag catcttcaca ccagtacggc tcgccgtaca tggtgtgaaa
     4741 attgaaaccg tattcgtggg ctttctcacg ccagtccggg cgctcggtaa tactgactct
     4801 ttccatcggt atcagccacc cattgaacga gaagaggtgc cggttgcgct acgctgcata
     4861 gtgctttgtt tggcaacaga ttcaccaaaa ccgccacggg taacggtagt ggtggtcgcc
     4921 ggttttggtg ccattgccgt cttcggtacg gtcatggtgc ggcctggctg ggctgcgcca
     4981 tagtttttac ccgtcgcgtc ggtatattta ccgtaagccg gactggccgg gtttttcgag
     5041 gagaacagcg gctgctgtgc aaatcccgcg ccgccgccca tcagacgccc catcatgtaa
     5101 ccggccatca gcggcatcca gaaactcccg ctggattgct gagcctgcgc ctggttttct
     5161 ggtgccatgc cagcctgggc tggtgcttgc tgacactgac cttcaccaaa ttcagcaaca
     5221 cagtcttcac gggtggcgta tttcggcgca gtacgttcgg cttctttcag cgcattgttg
     5281 tacgcggtgg tacattcggc gcttttgcct gggtttgcag ctgaacagtc gtcagcattt
     5341 tgatagagag acactgtttc atcactcttt tcacagccag ccagcataaa aacagtggca
     5401 accgcgagag cgactggtgt cagatggcgt gcgctccagt ttttgcggaa cgatgcgtgg
     5461 cgtatggatt ttgtccgttt catttttgtc ttccgggacc agtggtaaat acccatcaga
     5521 atagaggatg gctggtcgaa attgaagcga gaagggggaa gaatgcggca gataacccgt
     5581 atctttacgt tgccttacgt tcagacgggg ccgaagcccc gtcgtcgtca tcagttacgg
     5641 aaagggttat gaccgttact ggtggtagtg cgtgcggatg tttgctgaac gacgggtgcc
     5701 gggctatcag gcgcataacc atcagcaata gcattctgtt ccggcgtttg cggggcaacg
     5761 ttttccggat tagtggaaac cggtttgctc agcgcattgt tcagtgccag caaatcctgc
     5821 tcgttcaacg tacccagggc tgacttaata ttcagctgat taatcaggta gttataacgc
     5881 gcattcgcca gctcttgctt ggcgttgtac agcgtggtgg tcgcatccaa cacatcaaca
     5941 atggtacgcg taccgaccga gtagcccgct tccatcgcgt ctaatgagct ttgcgcggaa
     6001 actacggctt gtttgtaggc gttaatacta ctgatagaag cattaatgtt gttgaaggag
     6061 gaacgtacgg tttgcacgac gctacgatgt gcgctttcca gttgctcact ggcaccaaca
     6121 aagttgtact gtgcctgttt cacctgcgag ttaaccattc cgccctgata aatcggcagc
     6181 gagaagctca ggccaacttt gttctggccc atattgctat cgtcatactg ggtaccagcg
     6241 gcaccacggg ttttcgaacc gctataagag gtgtcagaaa tcccggtaga agccgttaaa
     6301 tccagagtcg gtaagtgacc atcctgcgcc tggcgaattt gctcgcgcgc caggtcctgg
     6361 ctcaagcatg cctgtaacag cgacaggttg cgtttttcgg cttctttcag cagcgcgtta
     6421 accggctgtg gtttgtcggt tttaaagttt tcgacattca gcgccgccag ttccggatag
     6481 tagttaccgg tgatctggcg cagctgctct accgcgttat caaggttatt acgtgcggtc
     6541 acttcgttcg ccagcacggt atcgtactgc gcgcgggcgt tctgcacgtc ggtgatcgct
     6601 accaggccca cgttaaaacg ttgggtggtt tgatctaatt gacggtagat cgcttctttt
     6661 tgtgcctgtg tataggaaag aacgtcaata gcattcaaca cgttgaaata agcggtcgcg
     6721 gtgttgagga tcaaggtttg ctgatcggtc tgatatgtga cgtcctgaat ccctgctgct
     6781 ttttcctgca gcgttaacgc acgccatttc gacatatcaa aaatggattg agttaactgc
     6841 agggacgcac tggtcgcgtt cgagttgatg ccgttcgcgt cgcggtagcc gttgctatag
     6901 gtgtaatctg cacctaaacc tagctgtggc agtaatggac tgcgcgcttc attaattttt
     6961 tcaaaggcag catcacgatc ggcggcagac ttacgcaatt ccgggttact aaggcgtgct
     7021 tgctgataaa cttgcatcag gttctcggcc tggctcaacg aactgaaccc agaaaggctc
     7081 aggccgataa gaatggggag caatttcttc atttgcattc cttgtggtga agcagtattt
     7141 agcgcgatca aactgtaaaa ttattgcgga ttctagcaga agccgctacc gcaaaaggtt
     7201 ggcgttacgt gccatagggc gttaatgtgc ctcaatttaa catatgactg ctcaaacggc
     7261 agtcaaacgt cgctgaaatt cacatttaat tcactattag tgccaggaca ttaacaatgc
     7321 ttaagccaga caacctgccc gttacatttg gcaaaaacga tgtagaaatt attgcacgag
     7381 aaacacttta tcgcggcttt ttttcattag atctttatag atttcgtcat cgtctattca
     7441 acgggcaaat gagtcatgag gtacggcggg aaatttttga gcgcggtcac gccgcagtct
     7501 tgctaccctt tgacccagtg cgtgatgaag ttgtgctgat tgagcagatt cggattgccg
     7561 cgtacgacac cagcgaaacc ccctggctac tggagatggt tgccgggatg attgaagagg
     7621 gtgaaagtgt ggaagatgtt gcccgtcgcg aagcgattga agaggcggga ctgatagtca
     7681 aacggaccaa accggtgtta agtttcctgg caagcccggg gggcaccagt gagcgttcgt
     7741 caattatggt gggcgaagtg gacgccacga ccgcaagcgg tattcatggt ctggctgatg
     7801 aaaacgaaga tattcgcgtt catgtggtaa gccgggaaca ggcataccag tgggtagaag
     7861 aggggaaaat cgacaacgca gcgtcggtca tcgctttgca atggctgcag ctgcatcatc
     7921 aagcgttaaa aaatgagtgg gcataaatga agcgttacac acctgacttt cctgaaatga
     7981 tgcgcctgtg cgagatgaac ttttcataat tgcgccgttt gttaccgcgc aattacgcac
     8041 ccggcgaaac tgtaagctat caggtggcaa acgcacaata tcggctgacg attgtggaat
     8101 cgacccgata cactaccctg gtgacaatag aacagactgc gcccgcgatc agttactgga
     8161 gccttccgtc aatgacggtg cgtctgtatc atgacgcgat ggtggctgaa gtgtgttcaa
     8221 gccagcagat ttttcgcttc aaagcgcggt atgattatcc taataaaaag ttgcatcaac
     8281 gcgacgaaaa gcatcaaatt aaccagtttt tagcggactg gttgcgatac tgtttagcac
     8341 atggagcgat ggcgattccg gtttactagc gtcgtgaaac ctaaggacac catttggaaa
     8401 gcctgttaac ccttcctctg gctggtgagg ccagaggaag gattttacaa attaccgata
     8461 ctcacctgtt tgcacaaaag cacgaagccc tgttaggggt aaacacctgg gaaagctacc
     8521 aggcggtgct ggaggcgatt cgtccacacc agcacgaatt cgacctgatt gtcgcgacag
     8581 gtgatttagc gcaggatcaa tcctctgcgg cctatcagca tttcgctgaa ggcatcgcaa
     8641 gttttcgtgc gccctgcgtc tggctgccgg gcaaccacga tttccagccc gcgatgtaca
     8701 gcgcgttaca ggatgcgggt atctccccgg cgaagcgggt gtttattggt gagcaatggc
     8761 aaatcctgtt gctggatagc caggtgtttg gcgtgccgca cggtgagctg agcgagtttc
     8821 agcttgagtg gctggaacgt aaactggccg atgcgccaga acgccatacg ttgctgctgc
     8881 tgcatcatca tccgctacct gcgggttgta gttggctcga tcaacacagt ctgcgtaacg
     8941 cgggcgaact ggataccgtg ctggcgaagt ttccgcacgt caaatacttg ctgtgcggtc
     9001 atattcatca ggagctggat ctcgactgga acggtcgccg cctgctggca acgccgtcga
     9061 cctgtgtgca gtttaagccg cactgttcca actttacgct ggatactatc gcgcccggct
     9121 ggcgtactct cgagttacat gctgatggca cgctgaccac cgaggtgcat cgcctagcgg
     9181 acacacgttt ccaacctgat accgcttcag aaggctactg atgtctacgc ttctttattt
     9241 acacggtttc aacagctcgc cgcgctctgc aaaagcgagc ttgttaaaaa actggctggc
     9301 ggaacatcac cctgacgttg agatgatcat tccgcagttg ccgccgtatc cttccgacgc
     9361 ggcagaactg ctggaatcca ttgtcctgga acatggcggc gattcgctgg gtattgtcgg
     9421 ttcgtcactg gggggatatt acgccacctg gttgtcgcaa tgttttatgc tgcccgcagt
     9481 ggtggtaaac ccggcggtac gcccgtttga attgctgacg gactatctcg gtcagaacga
     9541 gaacccctac accgggcagc aatatgtgct agagtcacgc catatttacg atctcaaagt
     9601 catgcagatt gacccgctgg aagcgccgga tttgatctgg ctgctgcaac agacgggaga
     9661 tgaagtgctg gattaccgcc aggcggtggc gtactacgct tcctgccgcc agactgtcat
     9721 agaaggcggc aaccacgcat tcacgggctt cgaagattat ttcaacccga tcgtcgattt
     9781 tcttggtctg caccatctct gacgatcaac tcgaattgct aacttaaacc atgacgcaaa
     9841 cttataacgc tgatgccatt gaggtactca ccgggcttga gccggttcgc cgccgtccgg
     9901 ggatgtatac cgataccact cgccctaacc atttggggca agaagttatt gataacagtg
     9961 tcgatgaagc actggcgggt cacgcaaaac gcgtggatgt aatcttacat gctgaccagt
    10021 cgttagaagt gattgacgat gggcgcggga tgccggtgga tattcacccg gaagaaggag
    10081 taccggcggt tgaactgatt ctttgccgtc tgcatgcagg cggtaaattc tctaacaaaa
    10141 attaccagtt ctctggcggc ctgcatggcg tggggatttc ggtagttaac gccttgtcga
    10201 agcgcgtaga agttaacgtg cgccgcgatg gtcaggttta taacatcgcc tttgaaaatg
    10261 gcgaaaaggt gcaggattta caggttgtcg gcacttgcgg taaacgcaat accggtacca
    10321 gcgtgcactt ctggccggat gaaaccttct ttgacagccc gcgtttttct gtttcacgcc
    10381 tgacgcatgt gctgaaggcc aaagcagtac tgtgccccgg cgttgagatt acctttaaag
    10441 atgagatcaa caacaccgaa cagcgctggt gctatcagga cggtctgaat gattacctgg
    10501 cggaagcggt aaacggttta ccgacgctgc cagaaaaacc gtttatcggt aatttcgctg
    10561 gcgatactga agctgtggac tgggcgctac tgtggctgcc ggaaggcggt gaactgctga
    10621 ccgaaagcta cgtcaacctt atcccaacga tgcagggcgg tacccatgtt aatggtctgc
    10681 gtcagggcct gttggacgcg atgcgtgagt tctgtgaata ccgcaacatt ctgccgcgcg
    10741 gcgtgaagct gtcggcggaa gatatctggg atcgctgcgc ctatgttctg tcggttaaaa
    10801 tgcaggatcc gcagtttgcc gggcagacca aagagcgtct ctcttcgcgt cagtgtgcgg
    10861 cattcgtttc gggcgtggtg aaagatgcct tcatcctgtg gctgaaccag aacgttcagg
    10921 cggcggagtt gctggcagag atggcgattt ccagcgccca gcgtcgtatg cgtgcggcta
    10981 aaaaagtggt gcgtaaaaag ctgaccagcg gcccggcgct gcctggcaaa ctggctgatt
    11041 gtaccgcgca ggaccttaac cgtaccgaac tgttcctcgt ggaaggtgac tccgcaggcg
    11101 gatctgccaa gcaggcgcgc gatcgtgaat atcaggcgat catgccgctg aaaggtaaga
    11161 tccttaacac ctgggaagtt tcttccgacg aagtgctggc ctcgcaggaa gtgcatgata
    11221 tttcggtagc gatcggtatc gatcctgaca gcgacgatct gagccagctt cgttatggca
    11281 aaatctgtat cctcgcggat gcggactctg atggtctgca cattgccacg ctgctctgcg
    11341 ctttgttcgt aaaacacttc cgcgcgttgg tgaaacacgg tcacgtttac gtcgcactgc
    11401 caccgctcta ccgtattgat ctcgggaaag aggtttatta cgcgctgacg gaagaagaga
    11461 aagagggcgt acttgagcaa ttaaaacgca agaaaggcaa gccgaacgtc cagcgtttta
    11521 aaggtctggg ggaaatgaac ccgatgcaat tgcgtgaaac cacgcttgat ccgaacactc
    11581 gccgtctggt gcagttgact atcgatgatg aagacgatca gcgtactgac gcgatgatgg
    11641 atatgctgct ggcgaagaaa cgctcggaag atcgccgcaa ctggttgcaa gagaaaggcg
    11701 acatggcgga gattgaggtt taaaggaaag aacattgccc ggtcaggatg gccgggcaaa
    11761 caaggcagga ttaaatccct ggctgcagaa tacggatatt catctccagc acgtcgcctt
    11821 ttacggcttt gctgtacgcc ttcatgtgcg gggtttgcag atgcgcttca aggtgcgcga
    11881 tgctttccca ctgctcaatc atcacgatag aatccggtgc catagactgg aaactcacgc
    11941 cagcagcgca atccaccatt ggcgcatagc cgtggcaacc ttcttctttc agtacggttg
    12001 gaacgatttt agcaaactga tccaataccg cctgacggtg atgttgacca ggacgagtac
    12061 ggatttctgc gattacggta agcatggtta actccttcta aagcctgagc tctagttaac
    12121 caaaaatttc cacaagatgc ttgcgatatt cttcagtata gcggggaaca tcaggcattt
    12181 ttatcacgtc attagcgata aatgtcggca gcggttccat accgaggaat tggtttgctt
    12241 tatggaacgg cagatacaca ccgtcaacgc caacgccgtg gaagaactga tctttttcgg
    12301 tgaaggcttc cattggtgcg ttccaggtta gagaaagcat atattttttg ccctgtacca
    12361 ggccgccgga accgtatttt ttcgacggat ctttgcgggt acgaccatcg ctggcataca
    12421 gagtcccgtg accttcggtg aatacatcat caatatattt tttcactgtc cacggcgcgc
    12481 ccatccacca gcctggcatc tgccagatca ccacatcagc ccagagaaag ttttgtactt
    12541 ccgctttgac attgtagtcg ctgtcggcgc gaacgatgcg gacatcatgc ccgaggtcgc
    12601 gcagtgtgcc atccgcgact tcggtcaggg tgtcgttcag ttgaccattg gagtgggcga
    12661 attttttcgc gccgttgata atcaggatgt tgctcatttt ttaacctcaa ggtgagacgt
    12721 ttgtcgacta tagtacgtca cagagcggta cggtgaaatt agcaaaatgt gcaaagtctt
    12781 ttgcgaattt agcaaaagtc tgatctagct ctgactcacc gatgcaggat aaatattttc
    12841 tgaaaatgac aatactgata gccattcctc ataattctta ctcgtaccgg aataatttac
    12901 acgtttggtt cggttttttt ggggattgaa aatatggctt tgaataagta aaagatgaaa
    12961 atatcaacgt gtcatgttga taattataaa tggattttta tgagcgtact gatatatgtt
    13021 aaagcagaaa attaaaacaa tatttgaagc attgctatat ataatgctta cttattggtt
    13081 aatagattct ttttttgctt tcaataagta tgactggatg cttgagtcgg gaggcaatat
    13141 ttgcagtata ccttccgttt caggcgaaga tcgtatattg caagcaatga ttgcagcatt
    13201 ttttcttctt acgccactta ttattcttat cttaagaaaa ctctttatgc gggaaatgtt
    13261 cgagttttgg ctatatgtat tctcgctcgt tatttgtctg gtttgtggtt ggtggctttt
    13321 ttggggacgg tttatatttt gctattagta aaatctatga gagtcgtttt aacggctctc
    13381 atagacagag aagttaccag cttaccttcg cctcaaatcc accttgttcc gcattcccaa
    13441 actcaacatt catgtcatgc agcttggcga ttcgctggac aatcgatagc ccaagtccac
    13501 tgccggtagc ggtttgtccg ggtgggcgat agaagcgttc gccaattcgc gccagtgcct
    13561 ctggtgtcac accggggccg ttatccctca cgatgaaatt atcagcattc agcgtgacgt
    13621 ctatcacgct gccctgtgga ctgtagcgca cggcgttatc cagcaaattt cgcaccaaca
    13681 aacttagcaa tagcggttgt ccggtgcgtt tgatgccgtg ggcattgagt gtcagtcgca
    13741 cgtcaatttt cgcctgctgc gccgtgtggt aaatatccat caccgacgat tgtaggagat
    13801 cttcaagcgg gatctccgcg acgtcctgaa ggttatccag tgagtccagc cgcgatagcg
    13861 tgagcagttg atcaaccaga cgagtagcgc gatcgatccc ggaatgtaat tggagcagtg
    13921 cttttttccg cgcctgcgga tcatcgtcag agagctgcgc aacttcggtt tgcactttca
    13981 gcgctgttaa cgggctacgt agttcgtgag ctgcatcgga ggtaaagcgt cgttctcgaa
    14041 ccatcatcgc atgtgtgcgg gcgaacagtt gatttagcga ctcaaccagt ggccgcactt
    14101 cgctgggtac gccagtcgcg tttagtggtt tttccgagtc agggtcacgc atacgtagtg
    14161 ccagcgccag tttgttcagc ggcgcgagtt cacgacccag tagtaccatc atgatgatta
    14221 acataatcgg cagtgcgacc agccacggga tcaattgccc ggcaacaatc gccagcgcca
    14281 tgtcttcacg gtattcccat tcctggccaa caacgatgcg atatttgcca tcaggtgagg
    14341 tcatccagac aaaacgccaa ggatcgtctt caccgaccag ttgcccgtca gcaaaacctt
    14401 cccgttgata gctataggga atatcttctc cgttatcgcc atcattaagg accattctgc
    14461 cgtcgtgggt aaagatggca aaggtcagcg catcgtcatc aacatgaccg tgttttaatt
    14521 tatttggcgt ctgtgccatg cgatccgccg cgttgatttc gttgagatcg agcgtactta
    14581 accgtttggc aaacagcatc agttgggtgt cgaacaattc atcgacgtta tccgttgttt
    14641 gtttccaggc gacaaagctg gaaagcagcc aggtcaccga ggccagaatt aaaaagatta
    14701 gcgtcagcct gacgcgcaga ctaagacgtt gggtaaattt catttctcac ctaatgtgta
    14761 gccaatacca tgcacggtac gaataaaatc actaccgagt ttgcgtcgca gatgatgcac
    14821 atgcacttca acggcattac tggtgacctc ttcgtcccag gtatacagtt tctcttcaat
    14881 cagtttgcgc ggcagtaccc gaccagcgtt acgcatcagt aattccagca gggcaaattc
    14941 ttttggtttc agtgttaagg gttcgccagc cagcgtggcg atacgtttgc cggggtcgag
    15001 catgacgtta ccgtggcgca gctcgttgct ggcctggccg ttggttcggc gcatcagagc
    15061 ctccagcctg gcggcgactt ctatcaacgc aaaaggttta cacagataat cgtcagctcc
    15121 cagacgcagc ccttctacac gttccgctaa cgcatcacgc gcggtcaggt tcagtaccgg
    15181 ctcacgctga cctttctctc gccattcgcg caaaatatcg cgaccatcca tgcctggtaa
    15241 ggttaaatcc aggatcaccg catcataagg tgcgctataa agcgcctctt ttccctgacg
    15301 accttgtgta aaccagtcga cactaaaacc cattttacta aggccagttt tgatgccgtc
    15361 accaatcagc atgtcatctt ctatcagtaa aattcgcatt ttttcatccc tgcgataacc
    15421 gtatttgctg ccagtaaacc gtgttgtaac gcgctctgta cagcattaaa ttttattttt
    15481 ttcccttaag aagttgttaa ggactatctt gttaaatgct cggaaacaga cattaaaggg
    15541 agtaataaac atgaaaaaat tcgcagcagt aatcgcagta atggccctgt gcagtgcacc
    15601 ggtgatggca gcagagcagg gcggtttttc tggcccatcg gcaacgcaaa gtcaggccgg
    15661 aggatttcag gggccgaacg gcagcgtaac gactgtagaa agcgtaaaat ccctgcgtga
    15721 cgacacctgg gtaaccctgc gcggcaatat cgttgaacgc atctctgacg atctctacgt
    15781 gtttaaagat gccagcggta ctatcaatgt tgatatcgat cacaaacgct ggaacggtgt
    15841 gacggtgacg ccgaaagata cggttgagat tcagggtgaa gtcgataaag actggaattc
    15901 tgttgaaatt gacgtcaaac agatccgcaa agtaaatccg taattgttac cgctcccggg
    15961 acgcgttccc gggaataatt tcgcagggag gcaaaatgac aaacctgaca ctggatgtaa
    16021 acattatcga tttcccatca atccctgtgg cgatgttgcc gcaccgctgt agccctgagt
    16081 tgctcaacta cagtgtggcg aaatttatca tgtggcgtaa agagacgggg ctttctcctg
    16141 ttaaccaaag ccagactttt ggcgtcgcct gggacgaccc tgccaccacc gcaccggaag
    16201 cgtttcgctt tgatatctgc ggcagcgtta gcgaaccgat tcccgataat cgttatggtg
    16261 tgagcaatgg tgaacttacc ggtggacgtt atgccgtggc ccgccacgtt ggcgagctgg
    16321 acgatatttc acacacgata tggggcatta ttcgccactg gctgcctgca agcggcgaga
    16381 aaatgcgtaa agcaccgatt ctgtttcact acaccaatct tgccgaaggg gtgacagagc
    16441 agcgactgga aacggatgtt tatgtgccgt tggcgtgatg gggaatcttt accggagtgt
    16501 gagccaaatc actcgcgata agtttgagcg aacaatgtca caggcgtatt gttgttgggc
    16561 ttataatttc tccggtaagg aataccatgt atacgcgaaa tttattatcc ggtgcgccgt
    16621 aagccgtctc gggggtatcc atgagcaagc cgcacttatg gcaggaggcg cggggcactg
    16681 gtggagacac agctaaactg ggctgaaccg tatacttaca gtcgactact ttgatgcttt
    16741 aggcttccct ggactcgcat aacctcaact tctcgaatcg cccggagcgg acccccatgc
    16801 cgagtggtgt ggcatgggcg caaccttgtg ctgccctcta tgccgatcaa gtctacagca
    16861 aagagttgta tatcaaggca cattgatgcc tttatcgccg tcggagcacg tccggcggcg
    16921 ctattcacgc tgatctcctg tgactcgacg cggcagataa tgtagtatct ccggcaatat
    16981 tgcccctttg aaggctggcg aataagttga ggaatcagaa ttaatgagcg atatggcaga
    17041 gcgccttgcg ctacatgaat ttacggaaaa cgcctactta aactactcca tgtacgtgat
    17101 catggaccgt gcgttgccgt ttattggtga tggtctgaaa cctgttcagc gccgcattgt
    17161 gtatgcgatg tctgaactgg gcctgaatgc cagagccaaa tttaaaaaat cggcccgtac
    17221 tgtcggtgac gtactgggta aataccatcc gcacggcgat agcgcctgtt atgaagcgat
    17281 ggtcctgatg gcgcagccgt tctcttaccg ttatccgctg gttgatggtc aggggaactg
    17341 gggcgcgccg gacgatccga aatcgttcgc ggcaatgcgt tacaccgaat cccggttgtc
    17401 gaaatattcc gagctgctat tgagcgagct ggggcagggg acggctgact gggtgccaaa
    17461 cttcgacggc actttgcagg agccgaaaat gctacctgcc cgtctgccaa acattttgct
    17521 taacggcacc accggtattg ccgtcggcat ggcgaccgat attccaccgc ataacctgcg
    17581 tgaagtagct caggcggcaa tcgcattaat cgaccagccg aaaaccacgc tcgatcagct
    17641 gctggatatc gtgcaggggc cggattatcc gactgaagcg gaaattatca cttcgcgcgc
    17701 cgagattcgt aaaatctacg agaacggacg tggttcagtg cgtatgcgcg cggtgtggaa
    17761 gaaagaagat ggcgcggtgg ttatcagcgc attaccgcat caggtttcag gtgcgcgcgt
    17821 actggagcaa attgctgcgc aaatgcgcaa caaaaagctg ccgatggttg acgatctgcg
    17881 cgatgaatct gaccacgaga acccgacccg tctggtgatt gtgccgcgtt ccaaccgcgt
    17941 ggatatggat caggtgatga accacctctt cgctaccacc gatctggaaa agagctatcg
    18001 cattaacctc aatatgatcg gtctggatgg tcgtccggcg gtgaaaaacc tgctggaaat
    18061 cctctccgaa tggctggtgt tccgtcgcga taccgtgcgc cgccgactga actatcgtct
    18121 ggagaaagtc ctcaagcgcc tgcatatcct ggaaggtttg ctggtggcgt ttctcaacat
    18181 cgacgaagtg attgagatca ttcgtaacga agatgaaccg aaaccggcgc tgatgtcgcg
    18241 gtttggcctt acggaaaccc aggcggaagc gatcctcgaa ctgaaactgc gtcatcttgc
    18301 caaactggaa gagatgaaga ttcgcggtga gcagagtgag ctggaaaaag agcgcgacca
    18361 gttgcagggc attttggctt ccgagcgtaa aatgaataac ctgctgaaga aagaactgca
    18421 ggcagacgcg caagcctacg gtgacgatcg tcgttcgccg ttgcaggaac gcgaagaagc
    18481 gaaagctatg agcgagcacg acatgctgcc gtctgaacct gtcaccattg tgctgtcgca
    18541 gatgggctgg gtacgcagcg ctaaaggcca tgatatcgac gcgccgggcc tgaattataa
    18601 agcgggtgat agcttcaaag cggcggtgaa aggtaagagt aaccaaccgg tagtgtttgt
    18661 tgattccacc ggtcgtagct atgccatcga cccgattacg ctgccgtcgg cgcgtggtca
    18721 gggcgaacca ctcaccggca aattaacgtt gccgcctggg gcgaccgttg accatatgct
    18781 gatggaaagc gacgatcaga aactgctgat ggcttccgat gcgggttacg gtttcgtctg
    18841 cacctttaac gatctggtgg cgcgtaaccg tgcgggtaag gctttgatca ccttaccgga
    18901 aaatgcccat gttatgccgc cggtggtgat tgaagatgct tccgatatgc tgctggcaat
    18961 cactcaggca ggccgtatgt tgatgttccc ggtaagcgat ctgccgcagc tgtcgaaggg
    19021 caaaggcaac aagattatca acattccatc ggcagaagcc gcacgtggcg aggatggtct
    19081 ggcgcaactg tacgttctgc cgccgcaaag cacgctgacc attcatgttg ggaaacgcaa
    19141 aattaaactg cgtccggaag agctacagaa agtcactggc gaacgtggac gccgcggtac
    19201 gttgatgcgc ggtttgcagc gtatcgatcg tgttgagatc gactctcctc gccgtgccag
    19261 cagcggtgat agcgaagagt aatatgccgg ggagggcgct gtttcctccc gctagcaagg
    19321 aacgccggat gaaacgttaa gtcgtcatcc ggcagcgtat tcaacccccg gaaacgataa
    19381 aaattccctt gaaaccgttg tttattcatg cgttgcgatt aacaatacgc ttttccagag
    19441 agcggctttt aacaatgccc taacctgatt tcaggtgacg tacaatgcca gtctctcaga
    19501 ccttcagagg gtgttatgct atatatcttt cgtcttatta ttaccgtgat ttacagcatc
    19561 ttagtctgtg tattcggctc catttactgc cttttcagcc cgcgtaaccc gaaacatgtg
    19621 gccacctttg ggcatatgtt tggccgtctt gcgccactgt tcggcctgaa agttgagtgc
    19681 cgcaaacctg cagatgctga aagctacggc aatgctatct atatcgctaa ccaccagaac
    19741 aactatgaca tggtgacagc atcgaacatt gtgcaaccgc cgacggtgac ggtaggtaaa
    19801 aagagcttgc tgtggatccc cttcttcggg cagttgtact ggttaaccgg caacttattg
    19861 atcgacagaa acaatcgcac taaagctcac ggcaccattg cggaagtcgt gaatcatttc
    19921 aaaaaacgcc gtatttccat ctggatgttc ccggaaggaa cccgcagccg tggtcgcggc
    19981 ctgctaccgt tcaagactgg agcatttcac gcggcaattg cggcgggcgt cccgattatt
    20041 cccgtgtgcg tctctacaac ttcgaataag attaatctta atcgactgca caacggtctg
    20101 gtgattgtcg aaatgctgcc gccaattgac gtcagtcagt atggcaaaga tcaggttcgt
    20161 gagctggctg cccattgtcg ttcgataatg gaacaaaaaa tcgccgagct cgataaagaa
    20221 gtcgcagaac gcgaagccgc cggaaaagtt taagtcggca atctgtattt ttgcggggaa
    20281 cactttcctg cacggtatta ctttagccag ttttacatgg agcaaatatg tcactcagtc
    20341 ggcgtcagtt cattcaggca tcggggattg cactttgtgc aggcgctgtt cccctgaagg
    20401 ccagcgcagc cgggcaacag caaccgctac ccgttccgcc gctacttgaa tctcgccgtg
    20461 ggcaaccgct gtttatgact gtacaacgtg cgcactggtc atttacgcca gggacacgcg
    20521 cgtcggtctg gggaatcaat ggtcgttacc tggggccgac tatccgcgtc tggaagggcg
    20581 acgatgttaa gcttatttac agcaaccgcc tgacagaaaa tgtctcaatg acggtggccg
    20641 ggctacaggt accaggcccg ctgatgggcg gtccggcacg gatgatgtcg ccaaacgctg
    20701 actgggcacc cgtactgcct attcgccaga acgcagctac tctgtggtat cacgccaata
    20761 ctcccaaccg cacggctcag caggtctata acggccttgc cggaatgtgg ctggtggaag
    20821 atgaagtcag caagtcgctg cctatcccca accattatgg tgtggatgat tttccggtca
    20881 ttatccagga taaacggctg gataactttg gtacgccaga atacaacgaa ccgggaagcg
    20941 gcggctttgt tggtgatacg ctgctggtta acggtgtaca aagcccgtac gttgaagtct
    21001 cgcgtggctg ggtgcgcttg cgactgctga acgcgtcgaa ctctcgtcgc tatcaactac
    21061 agatgagcga tggtcgcccg ttacatgtga tttctggcga tcagggattc ctgcctgctc
    21121 ctgtatcggt gaagcaactt tcgctggcac cgggcgagcg ccgcgagatt ctggtggata
    21181 tgagcaacgg cgatgaagtg tcgatcacct gtggcgaagc ggcgagcatt gttgatcgta
    21241 ttcgcggctt ctttgagcca tccagtattc tggtttctac cttggtgcta acgctgcgcc
    21301 caaccggcct tctgccgctg gtcacagaca gtcttccgat gcgcttgctg ccaactgaaa
    21361 tcatggctgg ttcgccaatt cgcagtcgcg atatcagtct gggtgatgac ccgggtatta
    21421 atggacagct gtgggacgtc aaccgtattg atgtcaccgc gcagcaagga acgtgggaac
    21481 gctggacggt acgcgcggac gagccgcaag cgttccatat tgaaggcgta atgttccaga
    21541 tccgtaacgt gaatggcgcg atgccgttcc cggaagacag aggctggaaa gataccgttt
    21601 gggttgacgg acaagtggag ctgcttgttt atttcggtca gccttcctgg gcgcacttcc
    21661 cgttctactt caacagtcag acgctggaaa tggcggaccg tggctcgatt gggcaactgt
    21721 tggtcaatcc ggtaccgtaa tcttcatact cgcccgagga gggcgagtat ggaatacctt
    21781 tcgatttctc tttcgttccg gcttataatc cccggccttt gattattttt ttatcatttc
    21841 tggaagcaaa atgagctcta tctccctgat ccaaccggat cgcgacctgt tctcctggcc
    21901 gcagtactgg gccgcctgtt ttggaccggc accgtttttg ccgatgtctc gtgaagagat
    21961 ggatcaactt ggctgggata gctgcgacat cattttggtt actggcgacg cgtatgtcga
    22021 tcacccaagc ttcgggatgg cgatttgcgg tcgtatgctg gaagcgcagg gctttcgcgt
    22081 cgggatcatc gcccagccgg actggagcag caaagacgac tttatgcgtc tgggtaaacc
    22141 gaatctgttt tttggtgtta ctgctggcaa catggattcg atgatcaacc gctataccgc
    22201 cgatcgccgt ttacgtcatg atgatgccta cacgccagat aatgtcgcgg gtaagcgacc
    22261 ggatcgcgcc acgctggttt atacccagcg ttgtaaagag gcgtggaaag atgtgccggt
    22321 gatcctcggc ggtattgagg ctagcctgcg ccgtaccgcg cattatgatt actggtccga
    22381 taccgtgcgc cgttccgttc tggtagattc gaaagccgac atgctgatgt ttggtaacgg
    22441 tgaacgtccg ctggtggaag tagcgcatcg tctggcgatg ggcgagccga ttagtgaaat
    22501 ccgcgatgtg cgtaataccg caattatcgt aaaagaggcg ctgcctggct ggagcggtgt
    22561 ggattccacc cgtcttgata ccccagggaa aatcgaccca atcccgcatc cgtatggcga
    22621 agatttgccg tgtgcggata acaaacctgt tgcgccgaaa aagcaggaag cgaaagctgt
    22681 aatcgtgcag ccaccgcgcc agaaaccgtg ggaaaaaacc tacgtattgc tgccttcttt
    22741 cgagaaagtg aagggcgata aagtgctgta cgcccatgct tcgcgcattc tgcaccacga
    22801 aaccaaccca ggctgcgccc gcgcattgat gcaaaaacac ggtgaccgct atgtgtggat
    22861 caacccgcct gctatcccgc tttctaccga agagatggac tgcgtttttg cgctgccgta
    22921 caagcgcgtg ccacatccgg cctatggcaa tgcccgtatt ccggcttacg aaatgatccg
    22981 tttttcggtc aacattatgc gcggctgctt tggcggctgt tctttctgtt ctattaccga
    23041 gcacgaaggg cgcattattc agagccgttc ggaagattcg atcattaatg agatcgaagc
    23101 gatccgcgac accgttccag gttttacggg cgtgatttcc gatctcggtg ggccaactgc
    23161 caacatgtat atgttgcgct gcaaatcgcc acgcgctgaa caaacttgtc gccgtttgtc
    23221 gtgcgtttat ccggatattt gtccgcacat ggacactaac cacgaaccga cgatcaacct
    23281 ctatcgccgt gcgcgtgatc tgaaaggcat taaaaaaatc cttatcgcct caggcgtacg
    23341 ttatgacatc gccgtggaag atccgcgcta tatcaaagag ttggcgaccc atcacgtcgg
    23401 cggttatctg aagattgctc cggaacatac cgaagaaggg ccgttgtcga agatgatgaa
    23461 gccgggcatg ggcagctatg accgctttaa agagctgttc gatacctact cgaaacaggc
    23521 aggtaaagag cagtatctga tcccgtattt catctctgcg caccccggta cgcgtgatga
    23581 agatatggtg aatttggcgc tgtggctgaa aaagcatcgt ttccgtctcg accaggtaca
    23641 gaacttctac ccatcgccgc tggcgaactc aaccaccatg tattacaccg ggaaaaaacc
    23701 gctggcgaag attggttata agagcgaaga cgtcttcgta ccgaagggcg acaaacagcg
    23761 tcgtttgcat aaagcgttgt tgcgttacca cgatccggca aactggccgt taatccgcca
    23821 ggcgctggaa gcgatgggca aaaagcatct gattggcagc ccgcgattgc ttagtgcctg
    23881 cgccaaccat tgaagagatg cgtgaagctc gtcgccagaa ccgcaatacc cgtccggcgt
    23941 tgactaaaca tacgccgatg gcgactcagc gtcagacgcc tgctacggca aaaaaagcgt
    24001 cgtctacgca atctcgtccg gtgaatgctg gtgcgaagaa acgccctaaa gcggcggttg
    24061 gacgttaaaa aaaatccccg gcaactgaca tgctgccggg gattttttta tcattctgcg
    24121 tacatacgca agcagcgcag gcaatcaaaa tagtcctgtg catcaagacg cgtattggct
    24181 ctgttagctt cggatttggc agcatagatt tgtgggcgta gtgctttcat atcctgctca
    24241 agccgggcga ttttggcatt taatgtttgt acattgctcg ccagttgatt gaccttttgc
    24301 tcattgacgc agccactgag caagcaggtt gccagaacaa cagctcccac ggtgaaaatc
    24361 gttttcatat agtgttccct tttatttatt gttccggcta attgttattt aattcagctc
    24421 attgctttaa tctggcggcg gtttttgcct caatcagctt aagtaggctg ccgctgttcg
    24481 ggcggataaa atcagcgatt tcccacttac cttcgcgccg ctgtaaaacc agctcttgct
    24541 cttcattgtt gccattggtc agcgtaaaac gaacatgaat gcgggcggaa tcggcggttt
    24601 tctccaggac tcgaatttca tcaacgcgtg aaaatagcga ctcttcgcca aaacagatcg
    24661 gattgtcgta aataaattgc cgttctattt gcatatcttc atcgggatta ttggccgtct
    24721 tgaaggtggt tttatagaag gcattaaccg cctggcggta agagtcggaa accacccacg
    24781 tccacggcca gctataatca gcaatatgtt caaagagttc agcaacaaag gattctggct
    24841 gttctttctg caacgaagct aaagcggcca gcgttttttc attctcgcta ttaactgctt
    24901 gtaagacgct gccatgattg ctgacaatat catcaatgac ccaacgacca ttttccgcca
    24961 ccattttcag tgtctgtgtg gtcttttctt tatcatcttt aaagatacga aagcgcacaa
    25021 cggcatcggc atgatcgacg tcagtttggg ttatcgctac gctttctaac accagatcgc
    25081 caaaatcctg acaatcacaa accggatcat aatccagcca gccaatattg cccggtagtg
    25141 taagattgtt gttcagggta agcgcctgtt gaatacgcgc tgaagttatc gcccgctctc
    25201 cggtttcacc gaaataaggc gtactggcat ctgatttata gttctgataa atctgccgga
    25261 ctgtttgttc tacggtctgt gaaggaggct gcgcgtgtac ggtaaaactt gccagggctg
    25321 ctaaaaacag aagtattatt ttcatctgtt tttcacctct ccgttgcgtt tatcccggat
    25381 atagatgagt tgttctttta agagcttccg gctctgcatg atgatgtcct tatatttggc
    25441 attcctgaac aaggaagagt aacaacgggc gggacgcgag gggaataaat gatttctgaa
    25501 aagtccggta gcggaacatt accgccaccg ggagaatttg cacgttagcc gccgaactgg
    25561 tcaggatcgg gaccgagacg cttgccctga tcgagttttg caattttgcc gagttcgtct
    25621 ttgtcgagac ggaaatccca gacatcaaag ttttcggcaa tacgtgaagg tgtgaccgat
    25681 ttcgggatca ccaccaggcc gctatccaga tgccagcgga taacaatctg cgccggggtt
    25741 ttgccgtatt tatctgccag atcgcgaatg actttctgat cgaaaacgcc ttttcctcct
    25801 tgcgctaatg ggctccagga ttcggtctgg attttgtgtg tcgcgttcca ggcgtgtagc
    25861 tggcgttgtt gcatcagcgg atgaagttcg atctggttta tcacaggcgt cacgccagtt
    25921 tcatcaatca ggcgttgcag gtgatggatc tggaagttgc acacgccgat gcttttgatt
    25981 aatccctctt tttgcaattc gatcatgcct ttccatgctt cgacataatg gtcgatagcg
    26041 ggaacgggcc agtgcattaa gtagaggtcg atataatcaa gctggagttt tttcaggctg
    26101 tcgagcaggg cttcgcgggg gcacttgtgg tcgtcgttcc acagcttagt ggtgatgaac
    26161 agttcttctc tgttgactga ggcatttttc agggctttgc cgacaccttc ttcgttcttg
    26221 taggccgcgg cggtatcaat cgagcgataa cccacttcta acgctttttg aatggcggtg
    26281 attacttcct cattacttgc ttgccagacg cccagtccca gctggggcat gacattgcca
    26341 tcctgtagct taataacggt tggattagcc atacgttcct cctttatatg aactcaccgg
    26401 agcaggctcc ggtgaggtgt tgtgaactta agtctggacg aaatgcccga aaacgaaagt
    26461 ttgaggcgta aaaagcttag cgggcggctt tgtatatacg gcggctgaca tccaacgtaa
    26521 tgtcatgatt ttcgcccagt tgggtcatgc cgtgctcttc cagttttttc agcaaagccg
    26581 ggatggagct gccgtccaga ccgtagtcgg agaggtgggt cggcacgcct aattgctcaa
    26641 agaaattgcg ggttgcggca atcgcggcgt caatacgctc atcgtcggaa ccttcagtga
    26701 tgttccagac gcgttcagca tattgcagca gcttagcgcg cttggtctcg cgtttttcat
    26761 tccacagtgc aggcaggacg atagccagtg tttgcgcgtg atccagaccg tgcatcgcag
    26821 tcagttcgtg gcccagcata tgcgttgccc agtcctgcgg tacgccagcg ccaatcaaac
    26881 cgttcagcgc ctgagtcgcc gcccacatga cgttggcgcg cacatcgtag ttttctggct
    26941 ctttcagggc tttcggacca tcttcgatta gcgtcagcaa aatgccttct gcgaaacggt
    27001 cctgaatttt ggcatcaacc ggtttggtaa catactgttc cacggtgtgt acaaaggcgt
    27061 ccactacgcc gttagccacc tgacgcggcg gcagggtgta ggtataaacc ggatcgagca
    27121 cggcaaatac cggctgaaca tgtgcagaat ggaacgcctg cttgtcgcct gtggttttac
    27181 gggagatcac cgcgcctgcg ttggattctg aaccggttgc tggcagcgtc agcacacagc
    27241 ccatcgggat ggcgctttta atctctttac cgcccgtttg cagaatgtgc cacggatcga
    27301 tattttccgg atagttagcc gctgcggcga taaatttggt gccgtccagt acagaaccgc
    27361 cgccaaccgc cagcaggaaa gtcactttct gttcgcgaac cagtttcacg gcgttcatca
    27421 gcgtttcata agccgggttt ggctcaatac cgccaaattc cagcacgtcc atgcctttca
    27481 gggcatccag aacttgatcg agaacgccgg tttttttcac gctgccgcca ccgtaggtaa
    27541 tcaatacgcg agcatcgtga ggaatttgtt cgcgtaaacc agcgattgcg cctttaccaa
    27601 acagaatgcg ggttggggtg tgcagattaa agttgttcat tacttgctcc ctttgctggg
    27661 ccaatatgag ggcagagaac gatctgcctg atgtttttca ttgtgatcgc cagcgcccta
    27721 gctctcaatg ctcatttctg ccaatgtctt gcctatttct ccagagtgct ggagaaatgc
    27781 tacaaaattg cgcacaatca aattgccgca ttattcctaa gaaattacgc gatatgaaac
    27841 gtgaagagat ttgtcgcttg ctggcggata aagttaataa actgaaaaat aaagaaaata
    27901 gtttgtcaga actgttgccc gatgtgcgtt tgttgtatgg cgagacgcct ttcgcacgta
    27961 caccggtgat gtacgagcct ggcatcataa ttctcttttc cgggcataaa atcggttata
    28021 tcaat
//
LOCUS       DADLTF010000039        27738 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.039, whole genome shotgun sequence.
ACCESSION   DADLTF010000039 DADLTF010000000
VERSION     DADLTF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.039"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1100)
                     /locus_tag="JN749_001604"
     CDS             complement(<1..1100)
                     /locus_tag="JN749_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAY7368059.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JN749_001605"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="JN749_001605"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HAY7368060.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JN749_001606"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="JN749_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HAY7368061.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JN749_001607"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="JN749_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HAY7368062.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JN749_001608"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="JN749_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HAY7368063.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JN749_001609"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="JN749_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HAY7368064.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="JN749_001610"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="JN749_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HAY7368065.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCATLNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JN749_001611"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="JN749_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HAY7368066.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHSDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /locus_tag="JN749_001612"
     CDS             complement(5804..7084)
                     /locus_tag="JN749_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7368067.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="JN749_001613"
                     /pseudo
     CDS             complement(7086..8574)
                     /gene="puuC"
                     /locus_tag="JN749_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="JN749_001614"
     CDS             complement(8724..9281)
                     /gene="puuR"
                     /locus_tag="JN749_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HAY7368068.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNVN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="JN749_001615"
     CDS             complement(9308..10072)
                     /gene="puuD"
                     /locus_tag="JN749_001615"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HAY7368069.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10284..11702
                     /locus_tag="JN749_001616"
     CDS             10284..11702
                     /locus_tag="JN749_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HAY7368070.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11851..12015
                     /locus_tag="JN749_001617"
     CDS             11851..12015
                     /locus_tag="JN749_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368071.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12005..13390
                     /gene="puuP"
                     /locus_tag="JN749_001618"
     CDS             12005..13390
                     /gene="puuP"
                     /locus_tag="JN749_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HAY7368072.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13524..13769
                     /locus_tag="JN749_001619"
     CDS             13524..13769
                     /locus_tag="JN749_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HAY7368073.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14082..15725
                     /gene="sapA"
                     /locus_tag="JN749_001620"
     CDS             14082..15725
                     /gene="sapA"
                     /locus_tag="JN749_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HAY7368074.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVDVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15722..16687
                     /gene="sapB"
                     /locus_tag="JN749_001621"
     CDS             15722..16687
                     /gene="sapB"
                     /locus_tag="JN749_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HAY7368075.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16674..17564
                     /gene="sapC"
                     /locus_tag="JN749_001622"
     CDS             16674..17564
                     /gene="sapC"
                     /locus_tag="JN749_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HAY7368076.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17564..18556
                     /gene="sapD"
                     /locus_tag="JN749_001623"
     CDS             17564..18556
                     /gene="sapD"
                     /locus_tag="JN749_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HAY7368077.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18558..19364
                     /gene="sapF"
                     /locus_tag="JN749_001624"
     CDS             18558..19364
                     /gene="sapF"
                     /locus_tag="JN749_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HAY7368078.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19432..19785
                     /locus_tag="JN749_001625"
     CDS             19432..19785
                     /locus_tag="JN749_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HAY7368079.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20153..20941
                     /gene="fabI"
                     /locus_tag="JN749_001626"
     CDS             20153..20941
                     /gene="fabI"
                     /locus_tag="JN749_001626"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAY7368080.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21087..22214
                     /locus_tag="JN749_001627"
     CDS             21087..22214
                     /locus_tag="JN749_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HAY7368081.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRENNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22282..24216
                     /locus_tag="JN749_001628"
     CDS             22282..24216
                     /locus_tag="JN749_001628"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HAY7368082.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24294..24396)
                     /locus_tag="JN749_001629"
                     /pseudo
     CDS             complement(24294..24396)
                     /locus_tag="JN749_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24450..26424
                     /gene="pdeR"
                     /locus_tag="JN749_001630"
                     /pseudo
     CDS             24450..26424
                     /gene="pdeR"
                     /locus_tag="JN749_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26572..26745
                     /locus_tag="JN749_001631"
     CDS             26572..26745
                     /locus_tag="JN749_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368083.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26835..27584
                     /locus_tag="JN749_001632"
     CDS             26835..27584
                     /locus_tag="JN749_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7368084.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT         6433 a         7193 c         7124 g         6988 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaatgatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggacgggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg atctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa ctgcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 gacgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctaataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgccctcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggcgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gagtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgcgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttggcgtcc agcagctcca gctgtttatt gccgtagcgt tcccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtatcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgca cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatccg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc
     8281 ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga
     8341 agagagtgac cagtcgccgc gttcaaatac gccgcgagct gcgctcaccg cacggtcgat
     8401 atcgacgctc ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt
     8461 ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc
     8521 aatggcgagg cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac
     8581 tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg
     8641 ggctgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt
     8701 acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg
     8761 caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt
     8821 tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt
     8881 actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg
     8941 tacgtttcaa agatcatcgc cagcgtgcga ttcgggttaa cgttatgaac cagcttcatt
     9001 gacaccccct gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca
     9061 tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc
     9121 ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg
     9181 tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt
     9241 tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact
     9301 gtagtggtca gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct
     9361 cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc
     9421 ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag
     9481 cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc
     9541 aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt
     9601 catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga tgctccagca
     9661 attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt
     9721 gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg
     9781 ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata
     9841 ggtgcggctg cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa
     9901 gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg
     9961 cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc
    10021 tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc
    10081 tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat
    10141 tttttcaatc tagcagtgga ttttcatttt tgcaaactaa atttaacatt tgacaaacat
    10201 ttggtttgca tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga
    10261 aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc
    10321 agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc
    10381 gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg
    10441 gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct
    10501 cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa
    10561 tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg
    10621 atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg
    10681 acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt
    10741 tcccggttgt agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag
    10801 gggatctgca accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt
    10861 actccgttga taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc
    10921 agttgcagct gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa
    10981 tcaaccttta ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac
    11041 gcctggtgcg tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt
    11101 atgaagagca cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg
    11161 agaatgtgct ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg
    11221 ggatgattga cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc
    11281 gccgcttcca gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca
    11341 ccgtcgccct gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg
    11401 cgggtgccga tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg
    11461 gccttgataa cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag
    11521 gcttaccctt cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc
    11581 tgcgccgcta tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc
    11641 tgttgcagtt tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt
    11701 aattcccctt gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt
    11761 aatccagaca tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa
    11821 cgattaagac tgtgacaagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag
    11881 ggaggctgcg tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg
    11941 ctaaacaggt ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc
    12001 aattatggct attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg
    12061 aaaatcactg aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac
    12121 cgtatttgat acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta
    12181 tttgctggcg ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca
    12241 gtttccggag gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg
    12301 atttatggtc ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt
    12361 gttggcgaaa atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt
    12421 aaccttcgtc gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt
    12481 caataccctg tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt
    12541 tcagggactg cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag
    12601 cgagaacgcg cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct
    12661 cggtttcgat gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc
    12721 gaaagccatc ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt
    12781 tatgcagctg ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga
    12841 aattgcgctg tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt
    12901 gaacacatta gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat
    12961 ggggcgtgac aatgtgtttc cagagcgcgt gtttggctat gtgcacccaa aatggcggac
    13021 tccggcactc aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt
    13081 agtcaccgcg acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct
    13141 gtcggtgttt aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt
    13201 ccactatttg ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct
    13261 agagtcaacg tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg
    13321 gtacctgatc cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag
    13381 cgaaacgtaa tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca
    13441 ttaactataa aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag
    13501 cgcattaacc ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg
    13561 ccgatgagta cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg
    13621 cccactactt ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag
    13681 cccaacatga aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag
    13741 agtttttgaa tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg
    13801 ttgtccggat aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct
    13861 ttaatgggtt tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata
    13921 gttatagaga aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac
    13981 caaccacacg gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa
    14041 tattagtaag atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt
    14101 ctcttttggt gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc
    14161 cgcatgctga tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct
    14221 ttaacccatc caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg
    14281 atcgactgct ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct
    14341 gggaagtact cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc
    14401 aaaaaaccga ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct
    14461 tccagcgaat ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct
    14521 acttcgacag cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata
    14581 ccgttgagtt ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt
    14641 atgcttcggt catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc
    14701 aactcgaccg tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc
    14761 aatttattcg cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg
    14821 tagtggtgga tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat
    14881 gcgacgttct ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct
    14941 tgcgtttaac gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac
    15001 cgccgctaaa taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc
    15061 tgatgcaatc catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct
    15121 cgtgggccta tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac
    15181 agttgaaggc gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc
    15241 aggcgtggaa ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg
    15301 ttgacgtaaa agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg
    15361 atatgagcca tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt
    15421 tcttccgtcc gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt
    15481 gcgatccgaa attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc
    15541 gtattgaagc ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc
    15601 tggcgtcgtc attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc
    15661 cgtttggtaa cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac
    15721 catgattatc ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac
    15781 cttcgttggc ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact
    15841 gtggaatgcc tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag
    15901 cattaacggt caaccaattg ccgaacagtt aaaagaggtc ttcccggcga cgatagaact
    15961 gtgcatcctc gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg
    16021 gattacgcgc cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc
    16081 aatcccggtg ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg
    16141 gttgcccgtt tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc
    16201 gttgattgat gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat
    16261 ccgccatatg atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg
    16321 cctgatgcgt atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac
    16381 ccgcggtttg tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc
    16441 ggttattcct cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga
    16501 aatggttttt agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga
    16561 ttacgcagcc atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt
    16621 gatttctgat attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt
    16681 acgatagcgt atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat
    16741 tttatagtga cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt
    16801 gtatttttgg cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat
    16861 tattgccgcc atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc
    16921 tcggacgtga tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat
    16981 ttgtggtgac gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga
    17041 cacacggcct ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt
    17101 cgctgctgtt ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt
    17161 ttgctgtctg gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg
    17221 acgaactgga aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata
    17281 ttctctggtt tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg
    17341 cactgtcgat ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac
    17401 tcccctcgcc tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc
    17461 cgtggactgt catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc
    17521 ttggcgatgg cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat
    17581 tcgtaacctg accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt
    17641 aagtatgacc ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa
    17701 gagtttgatt gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga
    17761 ccgtatgcgt tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact
    17821 ggttggccat aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga
    17881 acgtgtgggt cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg
    17941 gcagcgtttt ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa
    18001 agatcacaaa gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa
    18061 agtgatgata gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac
    18121 aaactcaatg gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca
    18181 aaacagcaat accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc
    18241 ggataaaatt aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt
    18301 ggtgacgatg ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg
    18361 cagcgcgatg ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga
    18421 acagttaccg attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat
    18481 tgtgacgcca cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa
    18541 catggagaaa gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt
    18601 tccgctaccg gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct
    18661 ttacgctacg tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca
    18721 cactggcaaa aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg
    18781 atcatccgct gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc
    18841 aggatccttc gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac
    18901 tgcgcctgaa caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc
    18961 gtatggttgg gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac
    19021 aaaaacagcg tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg
    19081 atgaagccct cgcttcactg gatatgtcga tgcgttcgca gttgattaat ctgatgctgg
    19141 agttacagga aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga
    19201 agcacattag cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca
    19261 ccgcggatgt gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact
    19321 ttggcgaagc attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct
    19381 tccagattat tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat
    19441 aaaataaaat caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc
    19501 tggcgatatc ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg
    19561 gggagctaca ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt
    19621 gggcagcatg atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag
    19681 ggctggaccg tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg
    19741 gagattattc tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc
    19801 gaccgagccg aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca
    19861 aggtctgaac catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt
    19921 tgctggggta acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg
    19981 gtaaggggaa aagcgccccc cctgccgcag cctgctccgg tcggacctgg caactatagc
    20041 tactcacagc caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga
    20101 cgaatcgcct gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt
    20161 tctttccggt aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg
    20221 tatcgctcag gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa
    20281 actgaaaggc cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg
    20341 cgatgttgca gaagatgcca gcatcgacac catgttcgct gaactgggga aagtttggcc
    20401 gaaatttgac ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga
    20461 ctatgttaac gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag
    20521 cttcgttgca atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac
    20581 cctttcctac cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa
    20641 agcgtctctg gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg
    20701 tgttaacgcc atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt
    20761 ccgcaaaatg ctggctcatt gcgaagccgt taccccgatt cgccgtaccg ttactattga
    20821 agatgtgggt aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga
    20881 agtagtccac gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata
    20941 atcgttctgt tggtaaagat gggcggcgtt ctgccgcccg cgttatctct gttatacctt
    21001 tctgatattt gttatcgccg atccgtcttt ctccccttcc cgccttgcgt caggataacg
    21061 atttccttta cgaccaagga gcgcccatgg aacaacgcca catcaccggc aaaagccact
    21121 ggtatcatga aacgcaatcc agtactacgg agtatgacgt tctgcctctg gtcccggaag
    21181 ccgcaaaggt cagcgatccc tttctgctcg acgtgatcct tgatgaagag acgctggccc
    21241 ccttcctttc atggctggtc cctgcgcgcg ttcttgcagt ggagttgttc cctgaccagc
    21301 ttaccgtgac ccgttcacaa accttcaccg cttatgaacg cttgtcgacg gccctgacgg
    21361 ttgctcaggt ttgcggcgtc cagcggttat gtaactacta ttcggcgcga cttacgccgc
    21421 tccccggacc tgattcctcc agggaaaata atcatcggtt ggcacaaatc acgcaatatg
    21481 cccgccaact ggctagctcg cctgctatta tcgacaaccg atcgcgccag catctgaatg
    21541 acgtcggtct tactgtctgg gactgtgtga tcattaacca aatcattggt tttattggct
    21601 ttcaggcgcg gacaattgcg acatttcagg cttatctcgg gcatccggta cgctggttac
    21661 ccgggctggc gatacaaaac tacgccgacg cgtcactgtt tgctgatgaa tcaatacgct
    21721 ggcgaagcag ctatgaggtg gaaaaactac ctgaagagca cacaaaaagt tcaactgcag
    21781 aactttgcca actggccgaa acacactctc tccaccctat ttcactttcc cttctcgaaa
    21841 agttgttaaa cagcacacgg gtcaatacac agccggataa tcagcttgcg gcgttgttat
    21901 gcgcgcgtat aaatggcagt cctgcttgtt tttccacctg tatggattca tcaaatgaat
    21961 ataaaaaaat cagcaccctt ctgcgcaagg gcgaaaatga aattaaccga tgggctgacc
    22021 gtcattctgt tgagcgcgct accgttcagg cgatacaatg gctgacccga gcacccgatc
    22081 gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca acgcagatta
    22141 ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa atcgcgttgg
    22201 gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca gccgctctaa
    22261 tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca gcttaaacag
    22321 caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga aaaaggcttt
    22381 ggcttcctgg aagtcgacgc acaaaaaagt tatttcattc cgccgccgca gatgaaaaaa
    22441 gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg tgaatccgca
    22501 gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt tcagggcaaa
    22561 aatgaccgtc tgaccatcgt tcctgatcat ccacttttaa aagacgccat tccttgccgc
    22621 gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc cgaaatgcgc
    22681 cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata catcactttt
    22741 ggcgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct ggaaaaagaa
    22801 gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga agatctgacc
    22861 tcgctggatt ttgtcaccat cgacagtgcc agcactgaag atatggatga cgcccttttc
    22921 gctaaggcgc tgccggatga caaacttcag ctgattgtgg cgattgccga tccaaccgcg
    22981 tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt caccaactat
    23041 ctgcctggct tcaacatccc tatgctgcct cgcgagcttt ctgacgatct ctgctcactg
    23101 cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc tgatggcacc
    23161 attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa gctggtgtat
    23221 gaccaggttt ctgactggct ggaaaatacc ggtgactgga agcctgaaag tgaagcaatt
    23281 gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg gcgtcataac
    23341 cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga aaaaggtgaa
    23401 gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga agaagcgatg
    23461 attgccgcta acatttgtgc ggcccgcgta ctgcgcgata agctcggttt tggcatctat
    23521 aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt gctgaaaacg
    23581 cacggtctgc atgtcgatgc cgaagaagta ctcacgctgg acggtttctg caaactgcgt
    23641 cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg cttccagtca
    23701 tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga agcatacgcc
    23761 acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct gctgaaagcg
    23821 gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca aatggccgaa
    23881 cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata cgcacgcttc
    23941 ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga tatcagccgt
    24001 ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc ggcacctttc
    24061 ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt acaaattaaa
    24121 ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga agtccgcatg
    24181 gaaacccgca gcattattgc gcgcccggtc gcgtaatctc ctttcacggc ccattcctca
    24241 tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat tttttaactg
    24301 actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata tataaaaaac
    24361 aatgcgttta tccgccgtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
    24421 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
    24481 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgctttgc
    24541 gcttttctac caccaaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg
    24601 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
    24661 atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
    24721 gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt
    24781 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
    24841 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg
    24901 tcattgggca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
    24961 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
    25021 cacgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
    25081 aaaacgagat ttttttaatc tgttccggta ccgacattac cgaagagcgc cgcactcagg
    25141 agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
    25201 tgcaggagtt aatcgatcac gctattaatc aggcagataa caataaagtt ggggttgtgt
    25261 atcttgattt ggataatttt aaaaaggtca acgacgccta tgggcatttg tttggtgacc
    25321 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
    25381 cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg
    25441 aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg
    25501 aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg
    25561 cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac
    25621 aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata
    25681 ccaacttgcg taaagcactg gaaaacgatc agttggttat cgaaaatcac ctagcgtggc
    25741 gaagtgcgca gtctggaagc actagtacgt tggcagtcac ctgaacgtgg gttgattcca
    25801 ccgttggact tcatttccta cgccgaagag tcagggctaa ttgtgccttt aggccgttgg
    25861 gtgattctcg atgtcgtacg ccaggtggca aagtggcggg ataaaggcat aaacctgcga
    25921 gtggcggtaa atatttctgc acgtcagctc gccgatcaaa ccattttcac cgccctgaaa
    25981 caggttctcc aggaactcaa ttttgaatac tgccctatag atgttgaact gacagagagt
    26041 tgtctgattg agaatgatga actggcactg tctgttattc aacaatttag ccaactaggt
    26101 gcgcaagtgc atctggatga ttttggtacc ggctactctt cactttcgca actggcgcgc
    26161 tttccgatcg atgccatcaa acttgaccag gtttttgttc gagatattca caaacaacct
    26221 gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg
    26281 atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat
    26341 gagcggcaag gatttttgtt tgccaaaccg atgcccgccg tcgccttcga acgctggtat
    26401 aaacgctatc tgaagcgcgc ataagtagat gaatatatat acttagcgct atatcccgtc
    26461 attatatatc gtattcctgg atgataatta gccgttgctg ttaaacactt ctcttttagc
    26521 ggcaattttt gcattattca ttttccccct tttgctataa ggatgtcctt gatgtctgaa
    26581 tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct tgtggcaggc
    26641 gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt gcgccaggtt
    26701 gctgaatcta cgccagacat cccgaagaca ccgtgggaga tttaacaatc gtgccttcag
    26761 gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat tataacgtta
    26821 taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc attgaccagg
    26881 gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt accattcgcc
    26941 aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc tttgcagttt
    27001 cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg aagcgtgaac
    27061 tcgccgagtt tgccgcgtca ctggttcaac cgggcgaaac catctttatc gaaaatggca
    27121 gcagcaatgc cctgctcgcc cggactctgg gggagcagaa gaaaaatgtc actatcatca
    27181 cggtcagcag ctacatcgcg catttgctga aagacgcgcc ttgtgaagtc attttgctcg
    27241 gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc cagtgcatcc
    27301 agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct gaaactggat
    27361 tcaccggtcg cgatatgatg cgcaccgatg tggtcaatgc cgtgctggaa aaagagtgcg
    27421 aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac tccatcggtc
    27481 ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc gatctgatgc
    27541 atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt cccttctcta
    27601 cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt gataattaag
    27661 actatttacc tgttattaac actctcaaga tataaaatta ttatcagcga tataacagga
    27721 agtaattatc acgtgcgt
//
LOCUS       DADLTF010000040        27536 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.040, whole genome shotgun sequence.
ACCESSION   DADLTF010000040 DADLTF010000000
VERSION     DADLTF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27536
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.040"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="JN749_001633"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="JN749_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HAY7368085.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1958)
                     /locus_tag="JN749_001634"
     CDS             complement(1614..1958)
                     /locus_tag="JN749_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HAY7368086.1"
                     /translation="MFFGGHVLRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAI
                     LSAAGNEYSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGA
                     STGYTVWQLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN749_001635"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="JN749_001635"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HAY7368087.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN749_001636"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="JN749_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HAY7368088.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLHDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /locus_tag="JN749_001637"
     CDS             complement(6144..6842)
                     /locus_tag="JN749_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HAY7368089.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN749_001638"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="JN749_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HAY7368090.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="JN749_001639"
     CDS             7537..8574
                     /locus_tag="JN749_001639"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HAY7368091.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN749_001640"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="JN749_001640"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAY7368092.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="JN749_001641"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="JN749_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HAY7368093.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN749_001642"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="JN749_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HAY7368094.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="JN749_001643"
     CDS             10745..11626
                     /locus_tag="JN749_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7368095.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JN749_001644"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="JN749_001644"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HAY7368096.1"
                     /translation="MTVTTFSELELDKSLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="JN749_001645"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="JN749_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HAY7368097.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JN749_001646"
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="JN749_001646"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HAY7368098.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="JN749_001647"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="JN749_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HAY7368099.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="JN749_001648"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="JN749_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HAY7368100.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="JN749_001649"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="JN749_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HAY7368101.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="JN749_001650"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="JN749_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HAY7368102.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTLTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="JN749_001651"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="JN749_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HAY7368103.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="JN749_001652"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="JN749_001652"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAY7368104.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="JN749_001653"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="JN749_001653"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAY7368105.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="JN749_001654"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="JN749_001654"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAY7368106.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="JN749_001655"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="JN749_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAY7368107.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="JN749_001656"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="JN749_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAY7368108.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="JN749_001657"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="JN749_001657"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HAY7368109.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="JN749_001658"
     CDS             24893..25273
                     /locus_tag="JN749_001658"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAY7368110.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="JN749_001659"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="JN749_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HAY7368111.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JN749_001660"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="JN749_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HAY7368112.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..>27536
                     /locus_tag="JN749_001661"
     CDS             26442..>27536
                     /locus_tag="JN749_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY7368113.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6793 a         6855 c         7221 g         6667 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tatttaccga aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccggcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccgctcc agctatcgtt agccatatgg ctgcatccag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagcgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgtgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc caggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttggtacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgta aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggcacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcact ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgacgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattaa aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagagc ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca tcatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggcttttg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccaccg caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccattgc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggcgatcgca
    15241 aataccagcc ccggaaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatctact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatgc tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagttcc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgctaac ttggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggaattg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgaggg cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtacgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcacctcca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcttg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggtta tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatac aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgcc actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatga caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac gactatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cattaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttcacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggagtggct cgccgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgctatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgacct caaagccaaa gtagcccggc agaagctgag cccggacagt ttcacaacct
    26881 acggggtgcg tatcgaccgg attaaagcaa cgttcggcga gcgtgaggtg ttcagcgacg
    26941 taacatacaa tcggctggtg gaagtgctgg acgagtggat cgccactcgc tcgaacaata
    27001 acgccctgga gttgtttgcc gagctccgtc ggttctggaa gttctgcgca cctactcttt
    27061 gcaacggccg caatgttgcc gccagtctgc cagatgatta tgtttccttc cgcgtacaga
    27121 aacctacccc cacacggctt tttaccgata ttgaatcaat cgcccgactc tggctcaatg
    27181 ttgctgcctg cacctctgta caccagaaga atgctgttcg cttcatgatc atcactggtg
    27241 ttcgtccgat taatgtccat aacctgcgct gggactacgt ttacgaggag gctggtgaaa
    27301 ttgtttatcc ggaaggggtt atcggcatgc gaggggctat gaaaacacaa aaggctttcc
    27361 gcctgccgat aacgcctgag atccggcgga ttatcgacga gcagaaagcc tggcgtgatt
    27421 cagttcctga gtgcaacagg gattatgtat ttttgcagcc acgtgatcca atgcagccat
    27481 tttcaaaacg atcactggat aagctggtga aaacatacag cccggacggg gctgta
//
LOCUS       DADLTF010000041        27079 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.041, whole genome shotgun sequence.
ACCESSION   DADLTF010000041 DADLTF010000000
VERSION     DADLTF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.041"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            231..1664
                     /locus_tag="JN749_001662"
     CDS             231..1664
                     /locus_tag="JN749_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAY7368114.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="JN749_001663"
     CDS             complement(1672..2730)
                     /locus_tag="JN749_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7368115.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="JN749_001664"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="JN749_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="JN749_001665"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="JN749_001665"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HAY7368116.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JN749_001666"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="JN749_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HAY7368117.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JN749_001667"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="JN749_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HAY7368118.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="JN749_001668"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="JN749_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HAY7368119.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="JN749_001669"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="JN749_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HAY7368120.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JN749_001670"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="JN749_001670"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HAY7368121.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JN749_001671"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="JN749_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HAY7368122.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFCCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JN749_001672"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="JN749_001672"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HAY7368123.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JN749_001673"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="JN749_001673"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HAY7368124.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="JN749_001674"
     CDS             complement(17699..18322)
                     /locus_tag="JN749_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HAY7368125.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="JN749_001675"
     CDS             18619..18894
                     /locus_tag="JN749_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368126.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="JN749_001676"
     CDS             complement(18971..19213)
                     /locus_tag="JN749_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HAY7368127.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="JN749_001677"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="JN749_001677"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HAY7368128.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="JN749_001678"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="JN749_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HAY7368129.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JN749_001679"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="JN749_001679"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HAY7368130.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKACVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22592..24289
                     /locus_tag="JN749_001680"
     CDS             22592..24289
                     /locus_tag="JN749_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAY7368131.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24304..24699
                     /locus_tag="JN749_001681"
     CDS             24304..24699
                     /locus_tag="JN749_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7368132.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSVRLPRR"
     gene            24657..25037
                     /locus_tag="JN749_001682"
     CDS             24657..25037
                     /locus_tag="JN749_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HAY7368133.1"
                     /translation="MATTADQRTPAATVTRENDTINLVKDERIIVTPINDIYYAEAHE
                     KMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYILR
                     LKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="JN749_001683"
     CDS             25050..25907
                     /locus_tag="JN749_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7368134.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..>27079)
                     /locus_tag="JN749_001684"
     CDS             complement(25910..>27079)
                     /locus_tag="JN749_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAY7368135.1"
                     /translation="MDIGGDKSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAI
                     LRAASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSV
                     CYIIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAH
                     QRGKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQ
                     ACECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDLSNKEQAIQFLCGNLGVNGRT
                     EHPFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEV
                     ELVIMLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
BASE COUNT         7452 a         6417 c         6052 g         7158 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgttgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagtggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaacaaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggtag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccctc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aatgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata ccccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ccgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattgattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgag atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggtgtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg gcgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgcggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaagc cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccaaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag cgtttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgcccct gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacaga aaatttttct
    22381 acgctaacaa gaaaattacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttc aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgcgt cggtattctc ggcggcatgt tgtgtgagac
    23041 gttgaccatg attctggtaa ttgtctgggc accaactacc gcgttgggga tcgatatcgt
    23101 ctctaaaatc ggcattccaa tgatcctcgg tagcgtctgt atcggcttta ttgtgcttct
    23161 ggtgcaaagc gttgagggcg aaaaagaggc cagcgccgcg cggcaggcca agctggcgct
    23221 ggatatcgcc aacaaaacgc taccgctgtt tcgccatgtc aatagcgagt cattacgcaa
    23281 ggtctgcgaa attatccgcg atgacattca cgccgatgcg gtggcgatta ctaataccga
    23341 tcatgtgctg gcctatgttg gcgtgggtga acataactat cagaatggcg atgacttcat
    23401 tagcccgact acccgtcagg cgatgaatta cggaaaaatc atcattaaaa acaatgatga
    23461 agcccaccgc acaccagaga ttcattccat gctggtgatc ccattgtggg agaaaggggt
    23521 cgtgaccgga acgctgaaaa tttactactg ccacgcgcat cagatcacct cgtcattaca
    23581 ggaaatggcg gtcggtctgt cgcaaatcat ctccacgcaa ctggaggttt cacgcgccga
    23641 gcagctacgt gaaatggcaa ataaggcaga gcttcgcgcc ctgcaaagca aaattaatcc
    23701 ccattttctg tttaacgctc tgaacgctat ttcatcgtca atccgtctga atccggatac
    23761 cgctcgccag ttgatcttta atctgtcgcg ttatctgcgc tataacattg aattaaaaga
    23821 cgatgagcaa atcgatatca aaaaagagct gtatcaaatt aaagactata ttgccattga
    23881 gcaggcccgc tttggcgaca agctgacggt tatctatgat attgatgaag aggtgaattg
    23941 ctgcattccc agcctgctga tccagccgtt ggtggagaac gccattgtcc acggtattca
    24001 gccttgcaaa ggtaaaggcg ttgtcaccat cagcgttgca gagtgcggaa atcgggtacg
    24061 cattgcggtg cgagataccg ggcacggcat cgatccaaag gtgattgagc gggtcgaagc
    24121 gaatgaaatg ccgggcaata aaattggcct gctgaatgtc catcatcgcg tgaagttatt
    24181 gtatggcgag gggctgcata tccgccgcct ggagccgggg acggaaattg cgttttacat
    24241 tcctaaccaa cgcaccccag tcgcctcaca ggctacgtta ttgctttgag ccggagtgat
    24301 attgtgaaag tcatcattgt tgaagacgaa ttcctggcac aacaggaact gagctggcta
    24361 attaaagagc acagccagat ggagattgtc ggcacctttg acgacggtct ggacgtgttg
    24421 aagtttttgc agcataaccg cgtcgacgcc atttttctgg atatcaatat tccgtcgctg
    24481 gatggcgtgt tgctggcgca aaacatcagc cagttcgccc ataaaccgtt tattgtgttc
    24541 atcaccgcgt ggaaagaaca tgcggtagaa gcgtttgaac tggaggcgtt tgactacatt
    24601 ctcaaaccat atcaggagtc acgtattacc gggatgctgc aaaaactgga agcggcctgg
    24661 caacaacagc agaccagcgt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taagatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccat
//
LOCUS       DADLTF010000042        26980 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.042, whole genome shotgun sequence.
ACCESSION   DADLTF010000042 DADLTF010000000
VERSION     DADLTF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.042"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(58..603)
                     /gene="hemG"
                     /locus_tag="JN749_001685"
     CDS             complement(58..603)
                     /gene="hemG"
                     /locus_tag="JN749_001685"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HAY7368136.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
     gene            complement(615..2066)
                     /gene="trkH"
                     /locus_tag="JN749_001686"
     CDS             complement(615..2066)
                     /gene="trkH"
                     /locus_tag="JN749_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HAY7368137.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     FALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2105..2719)
                     /locus_tag="JN749_001687"
     CDS             complement(2105..2719)
                     /locus_tag="JN749_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HAY7368138.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2719..4050)
                     /gene="pepQ"
                     /locus_tag="JN749_001688"
     CDS             complement(2719..4050)
                     /gene="pepQ"
                     /locus_tag="JN749_001688"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HAY7368139.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4240..6429
                     /gene="fadB"
                     /locus_tag="JN749_001689"
     CDS             4240..6429
                     /gene="fadB"
                     /locus_tag="JN749_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HAY7368140.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6439..7602
                     /gene="fadA"
                     /locus_tag="JN749_001690"
     CDS             6439..7602
                     /gene="fadA"
                     /locus_tag="JN749_001690"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HAY7368141.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7695..8396)
                     /gene="fre"
                     /locus_tag="JN749_001691"
     CDS             complement(7695..8396)
                     /gene="fre"
                     /locus_tag="JN749_001691"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HAY7368142.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8442..9935)
                     /gene="ubiD"
                     /locus_tag="JN749_001692"
     CDS             complement(8442..9935)
                     /gene="ubiD"
                     /locus_tag="JN749_001692"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HAY7368143.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            10102..10590
                     /gene="rfaH"
                     /locus_tag="JN749_001693"
     CDS             10102..10590
                     /gene="rfaH"
                     /locus_tag="JN749_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HAY7368144.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10587..11369)
                     /gene="tatD"
                     /locus_tag="JN749_001694"
     CDS             complement(10587..11369)
                     /gene="tatD"
                     /locus_tag="JN749_001694"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HAY7368145.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11411..12187)
                     /gene="tatC"
                     /locus_tag="JN749_001695"
     CDS             complement(11411..12187)
                     /gene="tatC"
                     /locus_tag="JN749_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HAY7368146.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12190..12705)
                     /gene="tatB"
                     /locus_tag="JN749_001696"
     CDS             complement(12190..12705)
                     /gene="tatB"
                     /locus_tag="JN749_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HAY7368147.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12709..12978)
                     /gene="tatA"
                     /locus_tag="JN749_001697"
     CDS             complement(12709..12978)
                     /gene="tatA"
                     /locus_tag="JN749_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HAY7368148.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTISDKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13057..14697)
                     /gene="ubiB"
                     /locus_tag="JN749_001698"
     CDS             complement(13057..14697)
                     /gene="ubiB"
                     /locus_tag="JN749_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HAY7368149.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14694..15299)
                     /gene="ubiJ"
                     /locus_tag="JN749_001699"
     CDS             complement(14694..15299)
                     /gene="ubiJ"
                     /locus_tag="JN749_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HAY7368150.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGIRKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15313..16068)
                     /gene="ubiE"
                     /locus_tag="JN749_001700"
     CDS             complement(15313..16068)
                     /gene="ubiE"
                     /locus_tag="JN749_001700"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HAY7368151.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16163..17590)
                     /gene="rmuC"
                     /locus_tag="JN749_001701"
     CDS             complement(16163..17590)
                     /gene="rmuC"
                     /locus_tag="JN749_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HAY7368152.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFCRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSC"
     gene            complement(17731..18492)
                     /gene="udp"
                     /locus_tag="JN749_001702"
     CDS             complement(17731..18492)
                     /gene="udp"
                     /locus_tag="JN749_001702"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HAY7368153.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            18754..19569
                     /locus_tag="JN749_001703"
     CDS             18754..19569
                     /locus_tag="JN749_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HAY7368154.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            complement(19608..21869)
                     /gene="metE"
                     /locus_tag="JN749_001704"
     CDS             complement(19608..21869)
                     /gene="metE"
                     /locus_tag="JN749_001704"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAY7368155.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            22105..23058
                     /gene="metR"
                     /locus_tag="JN749_001705"
     CDS             22105..23058
                     /gene="metR"
                     /locus_tag="JN749_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HAY7368156.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(22946..23845)
                     /gene="bioP"
                     /locus_tag="JN749_001706"
     CDS             complement(22946..23845)
                     /gene="bioP"
                     /locus_tag="JN749_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HAY7368157.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(23921..24721)
                     /gene="yigL"
                     /locus_tag="JN749_001707"
     CDS             complement(23921..24721)
                     /gene="yigL"
                     /locus_tag="JN749_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HAY7368158.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            complement(24729..25751)
                     /gene="pldB"
                     /locus_tag="JN749_001708"
     CDS             complement(24729..25751)
                     /gene="pldB"
                     /locus_tag="JN749_001708"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HAY7368159.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            25862..26482
                     /gene="rhtB"
                     /locus_tag="JN749_001709"
     CDS             25862..26482
                     /gene="rhtB"
                     /locus_tag="JN749_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HAY7368160.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(26544..>26980)
                     /locus_tag="JN749_001710"
     CDS             complement(26544..>26980)
                     /locus_tag="JN749_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY7368161.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
BASE COUNT         6501 a         7433 c         6862 g         6184 t
ORIGIN      
        1 cctgacaaga ggaaatttaa aataattttc tgaccgcgca ttttttattc tttatgctta
       61 tttcagcgtc ggtttgtcgg ttaaatgggc gatttctcgg gcgaaattcg ccacctgctc
      121 ccaatcggta tagacaactt ctttgcgcgt atccgtttca ccgcctgaca tcttcataat
      181 cagcttgagc ataaaacggt cgtaccagcg ataacgtggg taacgcagcg ccccggcaat
      241 gaccgcgcag cgatcgggac gccattgcga gttcatcaga aacttccgcg cgtagctgtt
      301 ggtctgtgga gtacgcttct ccggtttgcg cgccaccagg tttacggaat aaaaggcgct
      361 cggcatcgaa ttcagtcgcg tcgcatgttt tttgacaaat tcctggaacg cagagtggta
      421 gtgaccatag cgaatagaag caccaatgac cacacggtca tagttttccc attgtggttc
      481 ttcaatgcgg tgcacattgg cgacatccgt ctggatcccc agttctttca gttccgaagc
      541 caggtaggag gcaatctcgc gcgtttgtcc gtcccttgtt gagaaaagaa ttaatgtttt
      601 cacgtattac tccatcattc acgccagaaa gtcggggtaa agagcaccag caatgtaaag
      661 acctcgagac gaccaaacag catgttggca atcaggatcc atttagccac cgggttcata
      721 ctggtaaagt tatcagcaac cacgccaagc cctggcccca ggttattcaa tgtcgcaaca
      781 actgaggcaa aggcagaaaa atcatccacg cccgtggcga taatcgccag catactgaca
      841 ataaacacca atgcataggc ggagaaaaat ccccaaacgg cttcgaggat acgttccggc
      901 agtgcgcgat tccccagctt aatgctatag acggcgttcg gatgcaccag tcgtttcagc
      961 tcacggttcc cctgcttaaa cagcagcagg atgcggatca ctttcaggcc accgcccgtt
     1021 gacccggcgc aaccgccgat aaaagctgaa cataaaagca gtaccggcaa aaagagcggc
     1081 cagcgggcaa tgctgtcagt cgtaaacccg gcggttgtcg ccatcgatac cacctggaaa
     1141 aacgcctggt taattgtcat cagcgccgaa ctgtagacat tatgaaacca cagtacgagt
     1201 gtacaaataa ccaccagcgt aaactgtacg ccgataaaca tgcgaaattc cggatcgcgc
     1261 caataaacct tcagactacg cccacttaac agtgaaaagt gcagaccgta gttacagccg
     1321 gagatcagca ggaagatagc aatgatagtg ttaatagccg gactgtcgaa ataaccgata
     1381 ctggcatcat gtgtcgagaa gccgccaata gcgatagtcg caaagctatg gccgatggca
     1441 tcaaaggcat ccattccggc aaaccacaac gccagcgcac aggcgacggt cagcaagaca
     1501 taaatcaacc acagagtttt cgccgtttcc gcaattcgcg ggcgcatttt gttatctttc
     1561 agcgggccgg gcatttctgc gcgatagagc tgcatcccac ccacgccgag gataggcagt
     1621 atcgcaaccg ctaacacgat gatccccatc ccgccaaacc attgcagcat ctggcgataa
     1681 aagaggatgg catgagggag cgaatccagc cccaccagcg tagtggcccc ggtggtggtc
     1741 aggccagaga aagattcaaa aaacgcatcg gtaatcgtga ggttcgggct ttccgagaag
     1801 ataaaaggga gcgcaccgac actacccagc acggtccaaa acagcaccac tatcagaaac
     1861 ccctcacggg atttcagttc gcccttctct ttgcggttcg gccaccacag catagagcca
     1921 atggcgaggg cgaaaaaaaa ggtctgggta aaagcgcggc ccgctccatc ccggtagatg
     1981 agtgctacca gcccagggat aatcatggtc cctgaaaata agatgaccag tagtccaacg
     2041 attcgggtaa tggcgcgaaa atgcatctct gccgcttcct tagttctgca aaacgaagtg
     2101 gggattattc ttcaatcgct aacaattgca atgaaccacg gctaaaatcc gccagctttg
     2161 cggaaaattc agccactttc gccgccggaa gcgccacccg cagcagaacg aatgcctgat
     2221 aatcactgtt gataattttg ccgtcacact gccccagcaa cgcttcaatg ccggttaact
     2281 gactatattc acattgcaaa gtatattcgg ttaatggcgt cttgcgttgg gtcgttagct
     2341 ggcgcagcgc ctgattcacg ccgccgccat acgctttcac taacccaccg gtgccaagca
     2401 atatgccgcc gtagtagcgc actaccacag cggtaatttc cccgacgccg ctgcccatta
     2461 gctgggcgag catcggttta cctgccgttc ccgccggctc cccgtcgtca gagaaaccca
     2521 gctgttgaga atcatccggc gcacccgcca cccacgccac gcaatggtgg cgggcatcgg
     2581 ggtgttctgc ccgcaccgat tcaacaaacg ctttcgccgc ctcaacgcca tcggtatgcg
     2641 ccaacatcgt tatgaagcgg ctctttttga tctcttcaac gaccgtgacc ggtgccgcag
     2701 gaattaacca gctttccatc acgccagttt cagatcccgg gtcatgtttt ccacgttgtt
     2761 ttcgtggatc accacgttgt cttcgatacg aatgccgccg aacggtttca gtgcttcaat
     2821 tttctgccag ttgaagtgct tgctgaactg cccttcacgc cacggtgcca gaagcgattc
     2881 aatgaagtag atacccggtt cgatggttaa caccatgccc ggctggagaa tacgggtgca
     2941 gcgcaggtac ggatattttg ccggtgccgc gaggtgtgta ccgctatcat cctgcataaa
     3001 accggcgacg tcatgcacct gcaggcccag cggatggcca ataccgtgcg gcataaacgg
     3061 tccggtgaga tcgttttcga ccatcgcttc ttcactcata tcggtgatga tttgatgttt
     3121 acgcagcagt ttggcgatgc gctgatggaa ctggatgtgg taatccacat agctgacgcc
     3181 tgctttcatg gtggcgatca gccccagttg ttcatcattt acgtctttca ccagctgtgc
     3241 gtagtcgttg tcgctttttg ccgaccaggt acgggtcagg tcagccgcat agccgttata
     3301 ttcggccccg gcatccagca ggaagctgcg catctcttcc ggtgcctggt ggtccagttt
     3361 ggtgtaatgc aacaccgcag cgtgttcgtt aagcgccaca atgttgctgt aaggtacgtc
     3421 ggtatcacga tgaccggtcg cggtcagata ggcaatattg atatcgaact cgctcatgcc
     3481 agaacggaac gcttcttctg ccgcacgatg accattgacc gccattttct gcgcttcacg
     3541 catacaggcc agttcgtact cggttttgaa ggagcggtaa taatgcaggt agtcgatcac
     3601 ccctttcggg ttgatgttgc tggcctcaat acccagttgc agcgcacgtt ccggcaccgg
     3661 accgatataa ccgatattgc cgcgcgcagc aggcagcagg ctaccaatgc catcggcttt
     3721 cggcagcgtg atcacttcca catcttcagt ccagaaggag gtcggcagcg gttcgacgtt
     3781 gtgccagtaa tcaaccggca gatagaacca cagtttcggc ttgttcacgc catccaccag
     3841 caaccagcag tttggcacct gagttaccgg cacccacgct ttgaattgcg ggttcacttt
     3901 aaacggatag ggatgatcgt cgagaaaaac gttgaacagc tcgccggagt gaataagtaa
     3961 tgcatccagc ttgaagcgcg ccagcgcatc gcgagtccgt tcttgtaagg tagctatatg
     4021 atttttatag agcgaggcca gtgattccat tttttaccct tctgtttttt tgaccttaag
     4081 tctccgcatc ttagcacatc gttcatccag agcgtgattt ctgccgagcg tgatcagatc
     4141 ggcatttctt taatctttta tttgcatatt tttaacacaa aacacacact ccgatgcatc
     4201 tggtacgacc agatcacctt gcggattcag gagactgaca tgctttacaa aggcgacacc
     4261 ctgtaccttg actggctgga agatggcatt gccgaactgg tatttgatgc cccaggttca
     4321 gttaataaac tcgacactgc gaccgtcgcc agcctcggcg aggccatcgg cgtgctggaa
     4381 cagcaatcag atctaaaagg gctgctgctg cgttcgaaca aagcagcctt tatcgtcggt
     4441 gctgatatca ccgaattttt gtccctgttc ctcgttcctg aagaacagtt aagtcagtgg
     4501 ctgcactttg ccaatagcgt gtttaatcgc ctggaagatc tgccggtgcc gaccattgct
     4561 gccgtcaacg gctatgcgct gggtggtggc tgcgaatgcg tgctggcgac cgattatcgt
     4621 ctggcgacgc cggatctgcg catcggtctg ccggaaacca aactgggcat catgccgggc
     4681 tttggcggtt ctgtacgtat gccacgtatg ctgggcgctg acagtgcact ggaaatcact
     4741 gccgccggta aagatgtcgg cgcggatcag gcgctgaaaa tcggtctggt ggatggcgta
     4801 gtcaaagcag aaaaactgat tgaaggggcg atggcgattt tacgccaggc cattaacggc
     4861 gacctcgact ggaaagcgaa acgtcagccg aagctggaac cgctaaaact gagcaagatt
     4921 gaagccacca tgagcttcac catcgctaaa ggaatggtcg cgcaaacggc ggggaaacat
     4981 tatccggccc ccatcaccgc agtaaaaacc attgaagctg cggcccgttt tggtcgtgaa
     5041 gaagccttaa atctggaaaa caaaagtttt gtcccgctgg cgcataccaa cgaagctcgc
     5101 gcactggtcg gcattttcct taacgatcaa tatgtaaaag gcaaagcgaa gaaactcacc
     5161 aaagacgttg aaaccccgaa acaggctgct gtgctgggcg caggcatcat gggtggcggt
     5221 atcgcttacc agtccgcgtg gaaaggcgtg ccggttgtca tgaaagatat caacgacaag
     5281 tcgttaaccc tcggcatgac cgaagccgcg aaactgctga acaagcagct tgagcgcggc
     5341 aagatcgatg gtctgaaact ggctggcgtg atctccacga tccacccaac gctcgactac
     5401 gccggatttg accgcgtgga tattgtggta gaagcggttg ttgaaaaccc gaaagtgaaa
     5461 aaagccgtac tggcagaaac cgaacaaaaa gtacgccagg ataccgtgct ggcgtctaac
     5521 acttcaacca ttcctatcag cgaactggcc aacgcgctgg aacgcccgga aaacttctgc
     5581 gggatgcact tctttaaccc ggtccaccga atgccgttgg tagaaattat tcgcggcgag
     5641 aaaagctccg acgaaaccat cgcgaaagtt gtcgcctggg cgagcaagat gggcaagacg
     5701 ccgattgtgg ttaacgactg ccccggcttc tttgttaacc gcgtgctgtt cccgtatttc
     5761 gccggtttca gccagctgct gcgcgacggc gcggatttcc gcaagatcga caaagtgatg
     5821 gaaaaacagt ttggctggcc gatgggcccg gcatatctgc tggacgttgt gggcattgat
     5881 accgcgcatc acgctcaggc tgtcatggca gcaggcttcc cgcagcggat gcagaaagat
     5941 taccgcgatg ccatcgacgc gctgtttgat gccaaccgct ttggtcagaa gaacggcctc
     6001 ggtttctggc gttataaaga agacagcaaa ggtaagccga agaaagaaga agacgccgcc
     6061 gttgaagacc tgctggcaga agtgagccaa ccgaagcgcg atttcagcga agaagagatt
     6121 atcgcccgca tgatgatccc gatggtcaac gaagtggtgc gctgtctgga agagggcatt
     6181 atcgccactc cggcggaagc ggatatggcg ctggtctacg gcctgggctt ccctccgttc
     6241 cacggcggcg cgttccgctg gctggacacc ctcggtagcg caaaatacct cgatatggca
     6301 cagcaatatc agcacctcgg cccgctgtat gaagtgccgg aaggtctgcg taataaagcg
     6361 cgtcataacg aaccgtacta tcctccggtt gagccagccc gtccggttgg cgacctgaaa
     6421 acggcttaag gaatcacaat ggaacaggtt gtcattgtcg atgcaattcg caccccgatg
     6481 ggccgttcga agggcggtgc ttttcgtaac gtgcgtgcgg aagatctctc cgctcattta
     6541 atgcgtagcc tgctggcgcg taacccggcg ctggaagcgg cggccctcga cgatatttac
     6601 tggggttgtg tgcaacagac gctggagcag ggttttaaca tcgcccgtaa cgcggcgctg
     6661 ctggcagaag tgccgcactc tgtcccggcg gttaccgtca atcgcttgtg tggttcatcc
     6721 atgcaggcac tgcatgacgc agcacgaatg atcatgactg gcgatgcgca ggcatgtctg
     6781 gttggcggcg tggagcatat gggccatgtg ccgatgagtc acggcgtcga ttttcacccc
     6841 ggcctgagcc gcaatgtagc caaagcggcg ggcatgatgg gcttaacggc agaaatgctg
     6901 gcgcgtatac acggtatcag ccgtgaaatg caggatgcct ttgccgcgcg gtcacacgcc
     6961 cgcgcctggg ccgccacgca gtcggccgca tttaaaaatg aaatcatccc aactggtggt
     7021 cacgatgccg acggcgtcct gaagcagttt aattacgacg aagtgattcg cccggaaacc
     7081 accgtggaag ccctcgccac gctgcgtccg gcgtttgatc cagtaaacgg tacggtaacg
     7141 gcgggcacat cttctgcact ttccgatggc gcagctgcca tgctggtgat gagtgaaagc
     7201 cgcgcccatg aattaggtct taagccgcgc gctcgtgtgc gttcgatggc ggtcgttggt
     7261 tgtgacccat cgattatggg ttacggcccg gttccggcct cgaagctggc gctgaaaaaa
     7321 gcggggcttt ctgccagcga tatcggcgtg tttgagatga acgaagcctt tgccgcgcag
     7381 atcctgccgt gcattaaaga tctcgggcta atggagcaga ttgacgagaa gatcaacctc
     7441 aatggtggtg cgatcgcgct gggtcatccg ctgggttgtt ccggtgcgcg tatcagcacc
     7501 acgctgctga atctgatgga acgcaaagac gttcagtttg gtctggcgac gatgtgtatc
     7561 ggtctgggtc agggtattgc gacggtgttt gagcgggttt aaccacttgc cggatgcgac
     7621 atagatctaa atgccactga ataaagtttt agttgccgtt cttcccgcct gtcaggggcg
     7681 ggttttttta tatctcagat aaatgcaaac gcatcgccaa acaggcgatc ttcccgcgca
     7741 ttacgctcac tgcaaaacag gtcacgggca attttcgcca tctcaaaacg tccggcaata
     7801 tagatatcat gctctgccag cgtaccatga tcctgcaata ccgccgttaa cacggtacca
     7861 gtacgcccac gccagcctgc ttccggttgt tcaaccaccg gcaccacttg cagaccaggg
     7921 tgctttaacg aaagcgcctc aagctcgcag agatcataca gatgctgctc ttcacgcccg
     7981 ccccagtaaa tggtgatatc acggtttggg ttacgcgcca acgctgtcag caaaatcgag
     8041 cgggcataag agaacccggt gccgcccgca atcaaaatca tcggacgctc ttcatcatcg
     8101 cgcagccacg cttctccgtg gggaatgtcg accacgattt gatgatcttt gaggatgcgg
     8161 tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag ctcgataaac
     8221 cctttttcat ccggcgtcga ggccattgag aacggacgtt tgtcgcgctc atccatcact
     8281 accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggcacgat gcggacacga
     8341 tatacggtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt tgtcatgcgc
     8401 tttctctgtc ggatcgataa atagggcaaa acaaacgcgc atcaggcgct tttaccgttg
     8461 ttaaaaatag ccagttcatc ccagatggcg tcaatatacg tgacaacatc tggatctttt
     8521 ttgatgggac gtccccattc acgctgggtt tcccccggcc atttattcgt ggcatccagc
     8581 cccatttttg aacccagccc agagacaggc gaggcaaaat ccagataatc aataggcgta
     8641 ttttctacca gaacagtatc ccgcgccggg tccatacggg tggtaatcgc ccaaatcaca
     8701 tcgttccagt cgcgtgcgtt gacgtcatca tcgcaaacga tcacaaattt agtgtacata
     8761 aactggcgta agaacgacca gacgcccatc atgacgcgct tcgcgtgtcc ggcgtactgt
     8821 tttttgatcg tcactaccgc cagacgataa gaacagcctt ccggcggcag gtaaaaatcg
     8881 acaatttccg ggaactgttt ttgcagaatc ggtacaaaca cttcgttcaa cgccacgccc
     8941 agtaccgcgg gctcatctgg cggacgcccg gtataggtgg aatggtaaat cgcatcttca
     9001 cgctgggtaa tatgcgtcac ggtaaacacc gggaaactat cgacttcatt atagtaacct
     9061 gtgtggtcac catacggccc ttccggcgcc atctcaccag gatcgatata cccttccagg
     9121 acgatttcgg cactggctgg cacttcgagg tcattggaaa tacactttac tacttcggtt
     9181 ttggtgccgc gtagcaatcc ggcaaacgca tactctgaaa gcgtatccgg aacgggggtg
     9241 actgcaccga gaatcgtggc aggatcggca cccagcgcca cagaaaccgg gaaacgttcg
     9301 cccggatgcg ccgcacacca ctcctgataa tccagcgcgc cgccgcgatg cgacagccag
     9361 cgcataatca gtttgttttt accaatcagc tgctggcgat aaatgcccag attctgccgc
     9421 tctttatgcg ggccgcgcgt tacggtcagc ccccaggtaa tcagcggcgc ggcatcttcc
     9481 ggccagcagg tcataatggg aatgcgattg agatcgacgt catcgccaga gacgattttt
     9541 tgttggcagg gcgcaccacg cagtcgcttt gtcggcatgt ttaacacctg cttaaactgc
     9601 ggcagtttat caaacaggtc gcggaaacct tttggcggct ccggctcttt cagaaacgcc
     9661 aataatttac caacttcacg cagcgccgaa acatcttcct gccccatgcc catcgccacg
     9721 cgctttggcg taccgaacag gttgcacagc accggcattg agtagccttt agggttttcg
     9781 aacaacagcg caggcccacc agcacgcaga gtgcggtcag caatttcagt gatttccaga
     9841 tgcggatcca ccgggagcgt gatacgtttt agctcaccct gctgttcaag cagcgtcagg
     9901 aagtcgcgta aatcgttata tttcatggcg tccattgtag cctcttaatc tgcgcccatt
     9961 atacggcgtt catctttgca atgctgtaaa tttgttaaat tagcgtgaac tctgacggta
    10021 taacgcaaac cggggaatat aattaactta gcgtaaagct tttgctatcc ttgcgccccg
    10081 attaaacgga taagagtcat tatgcaatcc tggtatttac tgtactgcaa gcgcgggcaa
    10141 cttcaacgtg cccaggaaca ccttgagaga caggcggtta attgcctcgc tccgatgatc
    10201 accctggaaa aaatcgtacg gggtaaacgt accgcagtca gtgaaccgtt attccctaac
    10261 tacctgtttg tggaatttga tccagaagtg attcatacca cgaccatcag cgctacgcgt
    10321 ggcgtcagcc actttgtgcg ctttggcgcg tcgccagcga tagtcccatc ggcggtgatt
    10381 catcagctat cggtatataa accgaaagac attgtcgatc cggccccccc ttatccgggt
    10441 gataaggtga ttattaccga aggcgcgttc gaaggctttc aggccatttt caccgaaccc
    10501 gatggtgagg ctcgctccat gctattgctt aatcttatta ataaagagat taagcacagt
    10561 gtgaagaaca ccgagttccg caaactctaa aacgcaatcc caaacagtgt tttgacattg
    10621 gcatccgtgg tggcagccag ccatgcggca tcttctccac gccagtgcgc aatacgttgc
    10681 aaaatatggg gcagatgggc tggctcgttg cgccgggatg atggctttgg cgtgagatcg
    10741 cgcgggagca gatacggcgc atctgtttcg atcagcaatt tctccgccgg aatcaacggc
    10801 aacaattccc gcagctccag cccgcgtcgt tcatcgcaaa cccaaccggt aatgccgata
    10861 taaattccac gcgccacgca cgcctgcatc tcttcgcgtg tgccggtaaa gcaatgaaga
    10921 accgcaccag gcagtttatc cagccacggc tccagcaatg tcataaaccg ctcgtgggca
    10981 tcgcgacagt gcataaatac cggcatgttt aattctgcgg caatgcgtag ctgagcaaca
    11041 aaagcgcgtt cctgctcttc cggcgtcgaa aagttgcggt taaagtcgag accacattca
    11101 ccaatcgcca ccacttctgg ctgcgcggcc agctcaataa tcgcttcttc agtcgcagct
    11161 tgccactggc tgctgtcgtg aggatgaacg cccgccgttg accaacagga cgaatactga
    11221 cgcgccagct tttgcgcctg ctggctttca cgcaggttag tgccggtgat gagtagccca
    11281 ttaactcccg cgtcaaaagc gcgcgctaca acatcatcac ggtctttcgc aaattgcgaa
    11341 ctggtcaaat taacgccgat atcaaacatc ctgtactcca tatgacaacc gccctgacgg
    11401 gcggttgaat ttattcttca gttttttcgc tttctgcttc agcgtcgttt tcctcttccc
    11461 gatttcgccc tttaccaacg taaaagcgtg agaagaagac accgatttca aacagacagt
    11521 acatcgggat cgccaatagc gtttgcgaga agacatccgg cggcgtcagc aacatcccga
    11581 caacgaatgc accaaccagc acatacgggc gttttttgcg taagtcttct ggcgaggtaa
    11641 tccccatcca gcacagcagc acaattgcca ccggcacttc aaaggagaca ccaaacgcca
    11701 taaacagcgc cataacgaag cttaaatagc tggcgatgtc ggtggatacc tgcacccctt
    11761 ccggcgcggt attggcaagg aagccaaatg ccagcggaaa gaccacaaag taggcgaatg
    11821 ccatgccgat ataaaacagc agagagctgg aaaccagcag cggcaccacc aggcgacgtt
    11881 catgcttata cagcgctggg gcgataaatg cccacacctg atagagaatc accggcgctg
    11941 acagaatcag cgacaccata aaggtcagct tgatcggcgt aaagaacggc gaggccacgt
    12001 cggtggcgat catcgttgaa ccttgcggca actgcttgat caatggcgcg gataccaggt
    12061 gatagatgtc attggcgaaa tagaccagac acaggaatat cacgatcacc gcgataatgc
    12121 agttcagcag acgcttacgc agctcaatca gatgcgtgat aagcggttga gtatcttcta
    12181 cagacatgtt tacggtttat cactcgacga aggggaaggt gcagcggttt tcggttcagc
    12241 gtccgcagca ggttttacca ccggctctgg cgtggtttct ggcttctgtt ccggcgaact
    12301 ggcctgcgtt tgtgcagcgg caggcgttac gccctcatgc gcagcttcat tatctttcac
    12361 caccgggtta tggatggtgt gcgcttcatc gctcgccttt tcaggatcgt ttgcaacgta
    12421 ggaacgcttc atcgactccg cggcctggcg tagttcatcc atcgacgctt tcagttcggg
    12481 cgtcaggtta gtgaggctcg ccttttcaac ctttttcaga ctgtcctgaa actcctggag
    12541 ttttaactcc tgggtcagtt cgttctgcac cgttgtcgcc agtgaacgca acgcgcgaat
    12601 ccagcccgct accgttttta ccgccacagg cagtcgttgc ggccccagaa cgacgaggcc
    12661 gatgatgaac accaatagca gttcgctaaa accgatatca aacacggatt acacctgctc
    12721 tttatcgtgg cgcttcgcgt cttctgtttt agcctgttcc tgattcgtat ccgcctgctt
    12781 atcggagata gttttcgcag taaaatcagc atcctgactg gttttatcct gctttggttc
    12841 atcatcgctc attgcttttt taaagccttt gatcgacgca ccaagatcgg aaccgatgga
    12901 gccgagcttt ttggtgccaa aaagcagtac aacgatgacg gcaataatca ataactgcca
    12961 aatactgata ccacccatac atgttcctct gtggtagatg atgattaaac aaagccgcat
    13021 tatacgttac acggcccgcc ttgagcgatg aaaaaatcag cgtgttttgc gccaaccgac
    13081 aaaccaggcg atcagaccac ctgccattaa ccagccgggc atcagccccc actcaggtcg
    13141 gctgaccaac aagaatgtgc cacttaatac taacgtagcg ccaattccga gaaaataacg
    13201 cgattgtccc tgacgtacat gatttgactg aagctcgcgg gcaatcttat caacactgtg
    13261 ctgtaaatac ttgccctggc gcaaactgtc gtaaaccagt tcaggcagtt ctggcatttt
    13321 ttcgacccag aacggcgctt tttctttaaa tgctctcacc agggcaggaa taccgacctg
    13381 atctttaatc cacgactcca ggaaaggctt cgctgttttc cataaatcga gctgcggata
    13441 aagctggcgt cctacccctt cgacatagag cagggttttc tggagtaaca ccagttgcgg
    13501 ctgcacttcc atattgaagc gacgcgccgt attaaacaga tttaacagta catgtccaaa
    13561 cgaaatttcg gccagcggtt tctcaaagat aggttcacag accgtacgaa tggcaaattc
    13621 gaactcttca acgttggtat ctggtggcac ccagccagaa tcgacgtgta gctctgccac
    13681 tttgcgatag tcgcgattaa agaaggcgat aaagttttct gccagatagc gtttatcttc
    13741 tttgtttagc gagccaacaa tcccgcaatc aatgccgata tatttcgggt tttccgggtg
    13801 ttcatagctt acgaagatgt tgccagggtg catatcggca tggaaaaagc tgtcgcgaaa
    13861 gacctgagtg aagaacacct gcacgccgcg ttccgccagc aatttcatgt tagtgccgtt
    13921 tttctccagc gccgcaacat cagacaccgg aatgccgtaa atgcgctcca tcaccatcat
    13981 cccttcacta caatagtcag ggtaaacttc cgggatgtag agcatcgggc tgtcttcaaa
    14041 attgcgccga agctgaatgg cgttggcaga ttcccgcagc aaattcagtt catcaatcaa
    14101 ggtcttttcg tactcgcgca ccacttcggt tgggcgcaga cggcgaccat ccggcagcaa
    14161 acgcggcacc cagcgagcca gacggtagat aagtttcaga tccgctttaa tgaccggcaa
    14221 aatatccggg cggatgactt taatcaccac ctctttacca ttcgatttca atcgcgcggt
    14281 atgaacctgg gcgatagaag cagaagccag cggcttgatt tcaaaatcgt caaaccacgc
    14341 ttctaccggc aagccgccca ttgcagcttc aatctgctgc ttcgccagct tgccatcaaa
    14401 cggagcaact ttgtcctgca ataacgccag ctgatcggca atatgcggtg gaaaaagatc
    14461 gcggcgggtt gataacattt gcccgaactt gatccaaacc ggccccagtt cttgcagggc
    14521 cagtcgtagt cgctcaccta aaagtttgtc tttatgccga tttggcatcc agaataatga
    14581 gtatcgccat agccgtagcg gcagggtgat acgcattttg gggatcagtt catcaagtcc
    14641 gtagcttaaa aaagtgcgaa tgatgaaata taggcgccgt acttcaccag gcgtcattta
    14701 gcctccagtt tttccagccg tttggtcagg gcatcaacag cacgctcgac ggcagccgtc
    14761 tcttccgcaa accaggccac ttcaagcgga ccgggtgcca tacgccactc ttcagtaatg
    14821 gcttccgcca cataacgttg ctggcgctta atgccgtgat gcaggaactt tgcgcctccg
    14881 cgcatggctt ttctgattcc ttcagcggcg atatcaccgg tataaggggc cagcagttcc
    14941 gcagggtcga actctgccag atctgccagc gcaacgaagt tttgcaccac ctgaatatcg
    15001 ccctgcactt ccagctcacc actgcgaatc agtgcggtaa gctgctggcg atcgcgaagt
    15061 ttcggcaaca cactggcgta ggcgataacg gtgcagtcag catcgcctgc ccattcgccc
    15121 agtacatcaa cctggcgttc gctgaacacc agaatcaatg acgtcgaaaa gccttttacc
    15181 tccacgcgca atactttacc cagcagacgc gagcgggctg ttttcagcgc gggtgagcga
    15241 tacaggaagg tgttgagcag actttcaatt cctgccgtca ctaaaggttt aaaaggcatt
    15301 tccggtctcc tgtcagaact tataaccacg atgcagcgcc acaacccctg ccgtcagatt
    15361 gtagtagtcg acactttcga atccggcatc ctgcatcatg gctttcaggg tatcctgatc
    15421 gggatgcata cggatggatt ctgccagata acggtagctg tcggcgtcgt tcgcgaccag
    15481 tgagccaata cgcggcagca catggaagga gtatgcatca taggctttgc tcagcggctc
    15541 gataattggc ttcgagaact caagcaccag caggcggccg ccgggtttca gcacgcgata
    15601 cattgaacgc agtgctttat ctttgtcggt gacgttacgc agaccaaacg aaatggtgat
    15661 gcaatcaaag gtgttatccg ggaacggcag cgcctcagcg ttcgcctgaa catactcaac
    15721 gttgccaatc acaccgatat tacgcagctt ctcgcgtccc attttgagca tggattcatt
    15781 gatatcagca aggaccactt tgccagtttc tccgaccagg cgggagaatt tcgctgtcag
    15841 gtcgccggtg ccaccagcca gatccagcac ggtctgccca cggcgtacgc cgctgcaatc
    15901 aatcgtgaat cgcttccaca aacgatgaat accaaatgac atcaaatcat tcatgacatc
    15961 gtattttgat gccacggaat ggaaaacgtg ggcgaccata tccgcttttt gttccttcgc
    16021 gacggtctga aaaccaaagt gcgtcgtttc ttgtgactta tccaccatct caatgcctgc
    16081 tcatcaaaaa attgttccag aagtgtaaca gattgggcgt cgatgcccta gatttctacc
    16141 cggcttaact acccctaatg ggctagcacg actgctgatt atattcatca tcgcgttgat
    16201 aagcttcatc attcggctgc cccggaaccg accgaagtcg atactcttca tcctggctca
    16261 ccgcctgttc agccaaatcc ggattaatct cgcgtttaat ttctactcct aaaccgcgaa
    16321 acgcttctgc ctgcgccagc acatttccgc gacctgaaga gagttttttc attgcctggc
    16381 ggtagttatc ctgcgctttg tcgagacttt gaccaatcgc ggacatgtca tcgatgaaca
    16441 aacgcatctt gtcgtacagc ttgctggcac gatcggcgat ttgctgggcg ttacggcttt
    16501 gatgctcata acgccacagg ttggcaatag tacgcaacgc caccagcagc gtagtcgggc
    16561 taaccagcat gatattgttt ttcaacgctt cggtgatcag ctccggctgg cggtcaagcg
    16621 ccagtaaaaa agcgggttca acgggaataa acatcagcac gtaatccaga gttcgcagcc
    16681 ccggcagctg ttgataatct ttgcgtccca gcaaacggat atggttacgc accgacgcga
    16741 tatgttcctg tagcgcgctt tcgcgggtgt agtcgtcttc ggcgttaaaa tagcgttcat
    16801 aggcgaccag cgtcattttg gcgtcgatca ccacatcttt tccctgcggc agccgcacga
    16861 tgacatccgg ctgcatccgc gagcgggcgt cattttcgat gctgacctgg gtttcatatt
    16921 catacccttc acgcagaccg gaagcctcca gcacccgcgt caatactacc tcgccccagt
    16981 tgccctgggt tttattgtcg cctttcagcg cgcgcgtcag gttgatcgct tcctgggcca
    17041 tttgcgcgtt gagttgctgg agattgcgaa tttcgtgggt cagggtatgg cgttcttgtg
    17101 cttctttacc gaagctgtcc tgaacctgac ggcagaaacc gtccagttgt tcacgtagcg
    17161 gcgacaacag gctgttcaga ctctgacggt tttgctcatc aacccggcga ttgctgtgct
    17221 caaaaatacg gttggcgagg ttttcaaact gctcactgag gcgctgctcg ctgttaatca
    17281 tctggcgaat tttatcgtca gcatgttgct gtgcggcttc catccgcgtg gttacttcac
    17341 gcagatcggc ctccagagag gtgttaatac tttgcaggct gcgcacttcg ttattgagca
    17401 attcacactc tgcacgccag tgctcgcttt gggtaatttg ttgttttgcc gcgcttaact
    17461 ccgcgaccat ctcttcacgt tcagctaatt gctcggcttt ttgctgcgca tgttgataac
    17521 tggcaaacag ccagccaatt gccacaccca ccaacgcaat aactgcgtaa accatgattg
    17581 agaaatccac aacgcctcct gacgcaacaa cttacccgca caaaatagaa ttgtgctgta
    17641 caaacgtcca gttggaaagg catttcctgc gaggcgctac gcaaaaatac aaaaggccga
    17701 acgcgtcggc cttcagacag gagaagagaa ttacagcaga cgacgcgccg cttccaccac
    17761 gattttcacc gcatggcttt cagtttgttt catcgtctca gcattcggga tctcttgctg
    17821 ggtgcggtta acgataacac ccgctaccat accggcacgc aggccctgac ttgcacacat
    17881 ggtcagcaga gttgcagatt ccatttcata gttcattacg cccatcgcct gccactcttc
    17941 catagaacct ttaaagtgac gaactacgcg accagagtaa gtatcgtaac gttcctgacc
    18001 tgggtagaag gtatcagaag aaactgtcac gccaacgtga gttgtcgcgc caatagattt
    18061 cgcagcttca accagcgcag tcgtacattc gaaatcagcg acagccggga attccagcgg
    18121 tgcgaagtgc aggctcgcgc catccagacg gacagacgcc gtggtaacca ggacatcacc
    18181 cacattaata tgcggctgaa tagcgcccgt tgtaccgata cgcaggaagg tgcgaatgcc
    18241 cagctgtgcc agctcttcaa cagcaataga ggtagacggg ccgccgatac cggtagagca
    18301 gacgataaca ggtttaccat ccagctctgc acgccaggta gtgaattcgc ggtgagatgc
    18361 cagcttaacc ggcttatcca tcagcgcggc gatcttttcc acacgatccg ggtcgccagg
    18421 gacgatggca agcgtagccc cttgtaaatc gtttttagtg aggccgagat gaaaaacatc
    18481 agacttggac atatacaact cctctgtgaa tcggtttagt cagaagaagc aaaaagacac
    18541 tttaccgaag ggtttaacat tttttcgtga tactcatcac catgacgcaa atgcgttgca
    18601 taaatttacc caccaaaagt gattcatatc acataaatca ctcttcattc tgcaggactg
    18661 cataaaagat gagccgattc aggcaatgtg atttgttgca tgctgactat agttaacatt
    18721 gcgcaacatt tttataaggg tacggagaat accatggcaa caacacaaca atctggattt
    18781 gcacctgctg catcgcctct cgcttcgacc atcgttcaga ccccggacga cgcgattgtg
    18841 gcgggcttca cctctatccc ttcacaaggg gataacatgc ctgcttacca tgccagacca
    18901 aagcaaagcg atggcccact gccagtggtc attgtagtgc aggaaatttt tggcgtgcat
    18961 gaacatatcc gcgacatttg tcgccgtctg gcgctggagg ggtatctggc tatcgcacct
    19021 gaactttact tccgcgaagg cgatccgaat gattttgccg atatccccac gttgcttagc
    19081 ggtctggtag caaaagtgcc tgactcgcag gtgctggccg atctcgatca tgtcgccagt
    19141 tgggcttccc gcaacggcgg cgatgttcat cgtttaatga tcaccggatt ctgctggggt
    19201 ggacgtatca cctggctgta tgccgcgcat aatccacagc taaaagccgc agtggcgtgg
    19261 tacggcaaac tgacgggcga caagtcgctg aattcaccga aacaacctgt tgatatcgca
    19321 accgatctta acgcaccggt tctcggctta tatggtggtc aggataacag cattccgcag
    19381 gagagcgttg aaacgatgcg ccaggcgctg cgggctgcta atgcgaaagc agagattatc
    19441 gtgtacccgg atgccgggca tgcattcaac gctgattatc gcccgagcta tcatgccgaa
    19501 tctgcgaaag atggctggca gcgtatgctg gaatggttta agcagtatgg tgggaagaag
    19561 tcgctgtaat gagaaaagac cgggtggtat taccacccgg tttggattta cccccgacgc
    19621 aaattctgcg ccgcctgcac catgttcgcc agtgccgcgc gggtttctgg ccagccgcgc
    19681 gttttcaggc cacagtccgg gttgacccac aggcgctctg ccggaatgcg ttttgccgct
    19741 ttcttcagca aggcttcaat ccattccacg ctcggtacgt ttggcgagtg aatgtcatag
    19801 acgccaggac cgatttcatt tggataatca aactcttcaa acgactccag caactccatg
    19861 tcggaacgcg aggtttcgat ggtgatgacg tctgcgtcca gcgccgcaat cgaatccatg
    19921 atgtcgttga actcgcaata acacatgtga gtgtggattt gtgtgtcatc tttcgccacg
    19981 gcggcgttga tacggaaggc ctctacgccc cactggagat acgcatccca gtcgctacga
    20041 cgcagcggta aaccttcgcg cagcgccggt tcgtcaatct ggatgatgcc aattccagcg
    20101 gcttccagat cggccacttc atcacgcagc gccagcgcaa tctgtttggc gatggtttca
    20161 cggctgacat cttcacgcgg gaacgaccag cagagtatgg tcaccggccc cgtcagcatc
    20221 cctttcaccg gtttgtcggt cagcgattgc gcatacttcg cccactccac ggtaatcggt
    20281 gccgggcggc taatgtcacc aatgacaatc ggtggcttca cgcagcggga accgtagctc
    20341 tgtacccaac cgttttgcgt aaagacaaat ccgtcgaggt gctcgccaaa gtattccacc
    20401 atgtcattac gctcggcctc gccatgtacc agcacatcca gtcccaaacg ttcctgctca
    20461 acaatggcct gcttgatatg ttccgcaatg cccgtgcggt agttgttggc gtcgagattg
    20521 ccctttttga aatccagacg cagggtacga atttccgtgg tttgcgggaa ggaaccaatc
    20581 gtggtggtcg gccacgctgg cagtttaaaa cgcgcacgct gggcttcagc acgcacttca
    20641 tagacattcg cacgctggct gtcctgggcg gtgatcgccg ccagacgctt ttctaccgcc
    20701 ggattatgta cacgagtaga gtgacgacgc gcctgaattg gggcgctcca ctctgccaga
    20761 gctgccgtgt caccactgtt cagcgcatcg cgcagcagtg ccagttcatg gcatttttgt
    20821 agggcgaagg caaaccagct tttcacttct gcatcaagac gcgtttccac gctcaggtcg
    20881 atggggctgt gcagcaacga gcaggaagat gccacccaca aatcacgttt gccgacaatg
    20941 tccttaattt gcgcatattt ctcagtaaga tcggcgcgcc agacgttacg accattgatt
    21001 agacccgcag acagcagcca gtcagaaggc aggcgcttgt gcagttcaac aacgtcatct
    21061 ttaccatgta caaggtccac atgcagcccc tgaacaggca gcgcagtaat cgtgtcgaga
    21121 tttggcgtta cgccttcaaa ataggtggtc agcagcagtt tcacctgtcc ctggagcgcg
    21181 tcgtaagcgg gtttgtatgc gtccagccac gcctgtggta gttccagtac cagcgcgggt
    21241 tcatcaatct gtacccactc gatgccgcgt ttcgccagtt ctgccagcac ttgctgataa
    21301 accggcagaa tgtcgttcag caggctaagg cggtcaaact gttcaccttt cactttcccc
    21361 agccacaggt aagtaaccgg ccccagcagc acaggcttaa cgttgtggct cagcgccagc
    21421 gcctcgtcca cttcctccag cagctgcgtc caggtcagtt tgaactgttg gcctttaacg
    21481 aactccggca ccatgtagtg atagttggtg ttaaaccatt tggtcatttc cgctgccgcc
    21541 gcaggttcgc cagtcggcgc acgtccacga ccaatacgga acagggtgtc gatatctacc
    21601 gaaccgtctt tgttctgatg acgtggcgga acattaccca gcagcaaact ggtggtcagt
    21661 acatgatcgt accaggcaaa atcgcccacc ggcagcaggt cgatacccgc ttgcttttgt
    21721 tgatcccagt gacgagcacg cagttcacgc cctactgtca gcagttcttc acgcgtggag
    21781 ttccccgccc aataactttc ttgcactttt ttcagctcgc gacgcaggcc aacgcgaggg
    21841 aaaccgaggg tgtgattcaa tattgtcatt tttcttcctc taattatatg taaatcctat
    21901 ggattttgaa tttagggaag gcggcaagtt tattcatcgc cgggagctta ctgaagtaag
    21961 tgaccggggt ggagaaacgc agccaacgca ccataaattt aaaagacgaa ggattttagc
    22021 cgtccagatg tttacacatc cataattggc ggttactgta tattcctcaa gcgcaaatta
    22081 ttcatgccga agtgaaggac tttcatgatc gaagtaaaac acctgaaaac gctacaagcg
    22141 ttgcggaact gcggctcgct cgcagccgct gcggcaacgt tgcatcagac gcaatccgcc
    22201 ctgtctcacc agtttagcga tctggaacaa cgccttggct tccggctatt tgtgcgtaag
    22261 agccagccgc tacgctttac accgcaggga gaaatcctgt tgcaactggc aaaccaggtg
    22321 ctgccgcaaa tcagccaggc actgcaagcc tgcaatgaac cgcagcagac gcgtctgcgc
    22381 attgccattg agtgccatag ctgtattcag tggctgacac ccgctttaga aaatttccat
    22441 aagaactggc cgcaggtaga gatggatttt aaatcgggcg tgacgtttga cccgcagccc
    22501 gcgttgcaac agggcgagct ggatctggtg atgacctccg atatcctgcc gcgcagtggc
    22561 ctgcattatt cgccgatgtt cgattatgaa gtacgcctgg tgttagcacc tgaccatcca
    22621 ctggcggcaa aaacgcgtat tacaccggaa gatctcgcca gcgagacgct attaatttat
    22681 ccggtgcagc gtagtcgact ggatgtctgg cggcattttc ttcagccggc aggcgtcagc
    22741 ccgtcactga aaagcgtcga taacacctta ttattgattc aaatggttgc cgcgcggatg
    22801 ggtattgccg cgctaccgca ttgggtagta gagagttttg agcgccaggg tctggtggtg
    22861 accaaaaccc tgggcgaagg cttgtggagc cgactgtacg ccgccgtgcg cgatggcgag
    22921 cagcgtcagc cagttacgga agcgtttatt cgctcagcgc gcaatcacgc ctgcgatcat
    22981 ctgccgtttg tgaagagcgc ggagcgaccc acttacgatg cacccacagt gaggccagga
    23041 tcaccagcgc gcctgtaata aacgttggcc agtgcggctg ttggtgccag atagccaggt
    23101 ttaccagcag ccctgccgga acgtgcatat tattcataat gcccagcgtt ccggcgtcca
    23161 cctgcgtcgc gccgtagttc cacataaagt agccaatccc ggaagccacc acgccaagaa
    23221 acaccagaat gccccattgc aacgtggttt gcggcatttt ctgcgcattt cccagcaaga
    23281 accatgcaat taccgccacc aggaacgcgc caagatagaa ccacgcaaag gcgttatgct
    23341 gcggcatcgg gcgagtttcc atcaggcgtt tgtaacccac catgccgatg gcaaaagtga
    23401 tattggagag ttgcaccagc aacaagccag tccagaaatg gtcggtgacc tgatcatagc
    23461 gaataatccc ggcaccaatc accgccagca aggcgctaaa agcatagccc cagcgcagac
    23521 ggcgcttact catgatgtca taaatcagcg tgatgtagag cggcgtcagt acggtgaaca
    23581 gcaacaattc ggagaccgtc aggtagagat aagcgcggaa actcagcata tacatcacgc
    23641 cgagctgcat cgcgcccacc agcatataca ggccgaccgt tttcaggcta ttgctacggg
    23701 tacgcagaaa cggcagaaaa acgagtgccg ccaagccaac gcgcaccagc accgcaaaat
    23761 agctatcgac gtgccccgca aggtactcgc cataaaagct aaaggagaag gcccacagaa
    23821 tcgtggtgat gataagtagc gccacaatgg aaaactcttt gattaacgag tatccattgt
    23881 agcgaagtat caggttgaca actgaccaaa taaagaacga ttacgataaa tagagtttac
    23941 gcagataatg cggcaccgcg tcgtcggcat tagtaccaat aacttccagc tccggatgca
    24001 gatctttcag acgctggtgc gcactgccca taatgcagcc tttccccgcc atcgacagca
    24061 tttcggcgtc gttcatcccg tcaccaaacg caatacaatc cttcaggctg tagcccagtt
    24121 tcttcgccac tgcttccagc gcatggcctt tcgacacgcc gcccgccatc acttccagac
    24181 aggttaaggt agagaaactg acgttgacgc gatcgcccca gcgagcgtta atcgcctgct
    24241 ccagcggcag cagttgttca tgggaatcgc aggtgaaaaa cactttgctg acgccttccg
    24301 gctccagtaa tcccggctca tacagcgcat atttgaacac cgcttcttta aaaaagcgca
    24361 tctcttccgg gcgatggcga ttcataaacc attcgtcgtc gcgataaacg ttagtaatga
    24421 tgtccggatt gtcgttgact acgccaaaca gatcgctggc aatgtcgcga tccaggttat
    24481 gagcaaaaat cagattgcca tccagatcgt gaacgcgcgc accattggag gtaatcatgt
    24541 aagacttaat ctccagatta tcgcgaattt gccccacatc aacgtggtga cgaccggtcg
    24601 caaacacaaa gttgatgccg cgcgcggtga gcagcttcag agtttctttg gcgtaagggg
    24661 ataacgtatg gtcgggagaa agtaacgtgc catctaaatc agacgcaaca acctggtaca
    24721 taagaaattt aaacctctgt agagcggttt ccgctgggtg agttatgcct gttgaaaaaa
    24781 tcaacgatgg cgtggagcgc gactgagcgc attgcgtcct tttcaaaaag gatctcatgg
    24841 taagcacctt ttattaccaa cggccgtcct ccttcgacag gatggcccgc ggcggtgcgg
    24901 agttcacaaa aacggtcgtg catgcggtta tccaccacgc gttcctcttc agcttgtaag
    24961 agaagcgttg gcgtggcgtc atcacccgca ccggctaaca cctgttcgcc agccagaata
    25021 ctttcgcgta cccaatggta ggtcggccca ccgacgcgaa tcgttgggtc atcagcatag
    25081 aagcgtaagt tacgtcgata tcgctgtctg ctgtgggtca gtacgttgat agcaaacggc
    25141 aacgcgcgcc agcgcccggt gcctattgca tagccatcac ggaaacgtgg atgcgcttcg
    25201 gcccagttga ggatctgccg tgccataaat gacggcatac gaatcacgat cccaaacatt
    25261 ggcgcagtta gcgcaatggc gtcacataca cctggatggc gttgcagaaa taatgtggag
    25321 atcgcaccgc ccatcgaatg tgccagtata tagcgtttac gccacggacc gggctgaacc
    25381 tcctgctgcc agaatgccgc cagatcatca acataatcat taaagcgatt aacatgcccg
    25441 agatgcggat cggctaacag gcgaccggaa cgtccctgcc cgcgatggtc gatgattaag
    25501 acatcaaacc ccaaatggaa caggtcgtag gccagttccg catattttac gtagctctca
    25561 atacgccccg ggcagatgac taccacccgg tcatggtgct gtgcgcgaaa acggacaaag
    25621 cgcaccggaa tgtcatccac accagtaaac tctgcttcat cacgctgacg ccagaaatca
    25681 gtcagcggtc ccatggtaaa agcagcaaac gcgttttctc ttgtttccca gtctttttgc
    25741 tgctgaaaca tcgggtaatc tgcctcttaa accacgtaaa atcgtttttt taagcgtgcc
    25801 tgacacaacg ctgcgacagt agcgtattgt ggcacaaaaa tagacacacc gggagttcat
    25861 catgacctta gaatggtggt ttgcctacct gctgacatcg atcattttaa gcctgtcgcc
    25921 aggctctggt gcaatcaaca ctatgaccac ctcgctcaac cacggttatc gcggcgcggt
    25981 ggcgtctatt gctgggcttc agaccggact ggcgattcat attgtgctgg ttggcgtggg
    26041 gttggggacg ctattttccc gctcagtgat tgcgtttgaa gtgttaaagt gggcaggcgc
    26101 ggcttacttg atttggctgg gaatccagca gtggcgcgcc gctggcgcaa ttgatcttaa
    26161 atcgctggcc tctactcaat cgcgtcgaca tttgttccag cgcgcagttt ttgtgaatct
    26221 caccaatccc aaaagtattg tgtttctggc ggcgctattt ccgcaattca tcatgccgca
    26281 acagccgcaa ctgatgcagt atatcgtgct cggcgtcacc actattgtgg tcgatattat
    26341 tgtgatgatc ggttacgcca cccttgctca aaggattgct ctatggatta aaggaccaaa
    26401 gcagatgaag gcgctgaata agattttcgg ctcgttgttt atgctggtgg gagcgctgtt
    26461 agcatcggcg aggcatgcgt gaaaaataat gtcggatgcg gcgtaaacgc cttatccgac
    26521 ttactctgaa gacgcgtctg gcatcaccgc gaaataatca aatgaatgcc aaatccggca
    26581 aataacgccc cggcaaaacc atcaatccac ttcgccagac gttgataacc acggcgcatt
    26641 tgcggcaggg caaacagact ggcaacgacg gtaaaccacg ccagtgtttc gacaataatc
    26701 aacgcgaaaa tgccccagcg ctcggtagtg ccaacgttat cgccgacaaa caatgagaat
    26761 accgaaccaa aatagataat cgctttcgga ttggcgagat tggtcagtaa gcctttcagg
    26821 aaactgcgcc cgcttttcgc cagctcaacc tgtggtgcag gcgcagatac agcctctttt
    26881 ttcagtgcac cgcgtagcat ctggtaaccc atccagcaga gatacaggcc accgcccacc
    26941 ataatcagcg tatgcagcca ggccattttt tcgataatca
//
LOCUS       DADLTF010000043        25992 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.043, whole genome shotgun sequence.
ACCESSION   DADLTF010000043 DADLTF010000000
VERSION     DADLTF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.043"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            109..1092
                     /locus_tag="JN749_001711"
     CDS             109..1092
                     /locus_tag="JN749_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAY7368162.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            1115..2626
                     /locus_tag="JN749_001712"
     CDS             1115..2626
                     /locus_tag="JN749_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7368163.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2651..3649
                     /locus_tag="JN749_001713"
     CDS             2651..3649
                     /locus_tag="JN749_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7368164.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3682..4776
                     /locus_tag="JN749_001714"
     CDS             3682..4776
                     /locus_tag="JN749_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAY7368165.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGYRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            4788..5660
                     /locus_tag="JN749_001715"
     CDS             4788..5660
                     /locus_tag="JN749_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HAY7368166.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5708..6130)
                     /locus_tag="JN749_001716"
     CDS             complement(5708..6130)
                     /locus_tag="JN749_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7368167.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6227..7429)
                     /locus_tag="JN749_001717"
                     /pseudo
     CDS             complement(6227..7429)
                     /locus_tag="JN749_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(7439..8251)
                     /gene="hcaB"
                     /locus_tag="JN749_001718"
     CDS             complement(7439..8251)
                     /gene="hcaB"
                     /locus_tag="JN749_001718"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(cis-5,6-dihydroxycyclohexa-1,
                     3-dien-1-yl)propanoate dehydrogenase"
                     /protein_id="HAY7368168.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(8248..8568)
                     /locus_tag="JN749_001719"
     CDS             complement(8248..8568)
                     /locus_tag="JN749_001719"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HAY7368169.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            complement(8568..9086)
                     /locus_tag="JN749_001720"
     CDS             complement(8568..9086)
                     /locus_tag="JN749_001720"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HAY7368170.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(9083..10444)
                     /locus_tag="JN749_001721"
     CDS             complement(9083..10444)
                     /locus_tag="JN749_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HAY7368171.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            10580..11470
                     /gene="hcaR"
                     /locus_tag="JN749_001722"
     CDS             10580..11470
                     /gene="hcaR"
                     /locus_tag="JN749_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HAY7368172.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            11630..12769
                     /locus_tag="JN749_001723"
     CDS             11630..12769
                     /locus_tag="JN749_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HAY7368173.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(12761..14041)
                     /gene="csiE"
                     /locus_tag="JN749_001724"
     CDS             complement(12761..14041)
                     /gene="csiE"
                     /locus_tag="JN749_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HAY7368174.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRITRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTPQQEQIRKMLESA"
     gene            complement(14232..15086)
                     /locus_tag="JN749_001725"
     CDS             complement(14232..15086)
                     /locus_tag="JN749_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7368175.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMLPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            complement(15231..16034)
                     /gene="suhB"
                     /locus_tag="JN749_001726"
     CDS             complement(15231..16034)
                     /gene="suhB"
                     /locus_tag="JN749_001726"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HAY7368176.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGDL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            16153..16893
                     /gene="trmJ"
                     /locus_tag="JN749_001727"
     CDS             16153..16893
                     /gene="trmJ"
                     /locus_tag="JN749_001727"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HAY7368177.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            17163..17651
                     /gene="iscR"
                     /locus_tag="JN749_001728"
     CDS             17163..17651
                     /gene="iscR"
                     /locus_tag="JN749_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HAY7368178.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            17763..18977
                     /gene="iscS"
                     /locus_tag="JN749_001729"
     CDS             17763..18977
                     /gene="iscS"
                     /locus_tag="JN749_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAY7368179.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            19005..19391
                     /gene="iscU"
                     /locus_tag="JN749_001730"
     CDS             19005..19391
                     /gene="iscU"
                     /locus_tag="JN749_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HAY7368180.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            19408..19731
                     /gene="iscA"
                     /locus_tag="JN749_001731"
     CDS             19408..19731
                     /gene="iscA"
                     /locus_tag="JN749_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HAY7368181.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            19827..20342
                     /gene="hscB"
                     /locus_tag="JN749_001732"
     CDS             19827..20342
                     /gene="hscB"
                     /locus_tag="JN749_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HAY7368182.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            20359..22209
                     /gene="hscA"
                     /locus_tag="JN749_001733"
     CDS             20359..22209
                     /gene="hscA"
                     /locus_tag="JN749_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HAY7368183.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            22211..22546
                     /gene="fdx"
                     /locus_tag="JN749_001734"
     CDS             22211..22546
                     /gene="fdx"
                     /locus_tag="JN749_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HAY7368184.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            22558..22758
                     /gene="iscX"
                     /locus_tag="JN749_001735"
     CDS             22558..22758
                     /gene="iscX"
                     /locus_tag="JN749_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HAY7368185.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            22817..24100
                     /gene="pepB"
                     /locus_tag="JN749_001736"
     CDS             22817..24100
                     /gene="pepB"
                     /locus_tag="JN749_001736"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HAY7368186.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            24242..25018
                     /gene="sseB"
                     /locus_tag="JN749_001737"
     CDS             24242..25018
                     /gene="sseB"
                     /locus_tag="JN749_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HAY7368187.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDCALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25513..>25992)
                     /gene="sseA"
                     /locus_tag="JN749_001738"
     CDS             complement(25513..>25992)
                     /gene="sseA"
                     /locus_tag="JN749_001738"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HAY7368188.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
BASE COUNT         6148 a         6501 c         7216 g         6127 t
ORIGIN      
        1 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
       61 aaataattag aataatccgt tgttaataaa aaccctacag gaggcactat gcctaaaaaa
      121 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
      181 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccca gggatattac
      241 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
      301 attgaaacta acgcccaggg ggatatttcg aaagaaagta cctttgttga taccctcgtg
      361 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
      421 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
      481 aagggtgtcg ataaatatgt ctcggcgtat ctggtcggcg atccactgga atttggtaaa
      541 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
      601 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
      661 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta accaagaagg gactgtttta
      721 gataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
      781 atgggggagt cgggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
      841 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
      901 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
      961 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
     1021 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
     1081 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
     1141 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
     1201 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
     1261 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
     1321 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
     1381 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaatct
     1441 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
     1501 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
     1561 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
     1621 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
     1681 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
     1741 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
     1801 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
     1861 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
     1921 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgctac gtcgtggcga
     1981 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
     2041 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcatgcgccc
     2101 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
     2161 ggggatcatt ccctggctgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
     2221 cagcaccaac ggtgtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
     2281 gcggatgacg gtcaaggctg ccagtagcga aacacccatc ggcacgcttt ctggtggcaa
     2341 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
     2401 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
     2461 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg aattgccgct
     2521 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
     2581 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
     2641 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
     2701 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
     2761 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
     2821 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
     2881 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
     2941 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
     3001 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
     3061 actggggctg tggagcgctc tgcgcggaat ggggctgttt atgaccaacg cacagccagt
     3121 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
     3181 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
     3241 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
     3301 taacgttcgt cgagtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
     3361 cggcattttg ttggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
     3421 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
     3481 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
     3541 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
     3601 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
     3661 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
     3721 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
     3781 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
     3841 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
     3901 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
     3961 taccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
     4021 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
     4081 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
     4141 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
     4201 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
     4261 ctgggggcgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
     4321 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggggatggat
     4381 cacggctatt tagcaaccac cgaaggtctg ccgcagataa ttgctgaact tacccacggt
     4441 ggcgcggatg ttgcgctcga ttgttccggt aatgccgcag gtcgcttgct ggcactgcaa
     4501 tccactgctg actgggggcg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
     4561 gtcagcgctg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
     4621 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
     4681 attacccatc gcttctcgct agaacaggca ggtgatgcct atgtgctgat ggcgagcggc
     4741 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
     4801 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
     4861 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
     4921 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
     4981 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
     5041 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
     5101 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
     5161 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
     5221 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
     5281 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
     5341 aactggattt taaccagctc gcgccgttgc cgcgtcagtg ggtgaacaat ggttttgccg
     5401 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
     5461 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
     5521 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
     5581 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacctca gagatgtcgt
     5641 tgcgggtaga gtggctgtaa tttataaaat gccggataag gcgttcacgc cgcatccggc
     5701 atctttccta acgccgatcg agagaaattg cccccggtcc ggtaattgcc aatagcaaga
     5761 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
     5821 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
     5881 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
     5941 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
     6001 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
     6061 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacgta
     6121 gtgtgttcat aaccaatccc tttgcaagat aatgtgattt aaacgtaata cacactcttg
     6181 cacctcgcca cgccccaggc attgagatat tcgctaatta tcctggttac agtgatttaa
     6241 gcgcgatgtt ctcatctatc agcagtttcg catcaaacgt tttgccgctc tggatccatt
     6301 tgcgaattgg gcgaatctca cgcccctgat tcagcgtgac cgcaccgata agcacgccgt
     6361 tttgcagatt aaaccaaatc gccttctgag tttctgggtt gccacgacaa agctagtcat
     6421 cgccacgcat atcgccaata aactgtaagt tatcactgta ctgatcgctc cagaaccacg
     6481 gcggcggcag tagcggtagc ggtagcccta acattgcggc agcggcaatt tgcgcctggt
     6541 tattggcgtt ttcccagctt tcgcagcggt gtagtgcacc attatcaaga cgagtgattg
     6601 ccacatcgcc accggcaaag atcgcgggat cgcaggtgcg gcaagcctca tcaatgacaa
     6661 tgccattggc agtatcaagg ttggcctcgc gagccagttg ctcgttggcg ctgatactaa
     6721 taccgtaaat caccacatca gcctgaagcg tttccccact ttgcagcgtc agttctactt
     6781 tttcaccatc gaccacatgt tcaatggcat tattgagcag aatgcgcaca ccagcctgct
     6841 gatggcgctg taaaagatag cgttgcacgg gcggtggtgc attacggccc atgacggttg
     6901 ccgccagttc aatcactgtc accttacatc tgcgctgcgt ggcgctggca gccagttcca
     6961 gaccaatagt tccggcaccg acaatcacga ctgaccgttc gggctgcaga acttctcgca
     7021 gtctggcggc atcaccggca tggtgtaggg taaagcagcg ttctcccagt gcatcaagca
     7081 acggcagcgg tcgagctgcc gcgccggttg ctataaaaag ctgatcccag tgccagcttt
     7141 cgccgttggt taacactaac tctcgtgtgt cgcgacccag cgttttgatg gttacaccgg
     7201 aatgcagatg aacattgttt tcctgccacc agttagcggg taacacctgc tgtaactgcg
     7261 gggaatcttc cagcaacatg gatttcgaga gtggaggtcg ttcataagga agatgtcgct
     7321 catcggaaaa cagatgcagc tcaccggtga acccttgctg gcgtagcgag gccgcagcca
     7381 ttgccgccgc ttgcccgcca ccgacaataa tgatcgtttt ttctttcatc gtgcttcctt
     7441 aaagatccag cccagccgct acgtggcgaa tgccgcgaat cgctaaaccc gcatcagcgt
     7501 tgatcatcac accgcttaat gcgcgattat tgcgccgcga tgccaacatc acatacggcc
     7561 ccgtaaaatc cgccggttgc gggaaaaatt gcagcggtaa aatggcggca attttctccg
     7621 gcgtcagaga ctgcattatc gaggtttcac tttgcccgag cgcctgtggg ccgcgcaggt
     7681 cgctggccat accacacggg ccgacgccat tcacccgcac tttcggtgcc agttcataag
     7741 ccagttggcg aataagtccg gttgcggcat gtttactggt ggtgtacagc gggccaccgc
     7801 cgccaggata ccaggcggca tttgacagtg tgaaaatcat gctgccttca ctggcgatta
     7861 acgccggagc gcaggctttt gcgcccagca ggtaaccgag tacgttgacg ttaaacagct
     7921 cgtggaagcc ggtttcgagc gtctctgcgg gagtattaac cagtgaggca ttgtgatccc
     7981 agatgcctgc attgccgata aaacaatcca gcttgccgga acgagtcagg atctgatcga
     8041 ccgcgcgttg ataatcggca taacaggtca cgttaccttc caccgccaga atatgttcgc
     8101 caaatcgctg acgcagactg gcgacttttg ccgccgacag ttccagcgcg gcaacctgcg
     8161 cgccttcttc gataaatcgc tcgaccagcg ccagccctaa tcccgatccg ccgccggtaa
     8221 taaaaatgga ctcgttatgc agatcgctca tggctgcgcc tccggtaagt cgatgaaaat
     8281 gtcaccacct tcaacgtgta ctggataagt ggtgagcgga tcggtggcgg gcaggcataa
     8341 cgctttcccc gttttcaggc aaaaactggc ggcgtgtagc gggcactcca ccgtggcgtc
     8401 atcttccaga tacccttctg acatcgacgc attaccatgg ctgcaacgat cgttaatggc
     8461 ataaaactcg ccgcccacgt tgaacaaggc gatgacgggc gaggtatcta tccggagagc
     8521 ctcaccctcc ggcacatccg caacgggaca cgcataaatt cgattcatca gaacagtaca
     8581 ctcaggttat gggaagtgat caccgcttga tccaggacga tatcccgttc cagcaagcgc
     8641 cagttgtcat cttccagacg gcgaactttg tcgaaacgcg ttccaacata gaatgtttca
     8701 tcgcgctctt tttgtgcccg ataaagtaga taattcaccc tcacggcaaa tacgtttggg
     8761 atgtcggttt cgcttacctg gcagttgctg attaagtgac gagtgcgtga cggcggctct
     8821 tctgcccagg ccatgcccgt ttccagacgg gcgattcgcc gctccagctg gtctttggtg
     8881 tcattgaaaa tccaggtcgt cggcggctgg acgcctttgc gacggtcgcg tgtttgcgcg
     8941 ttaaccgtgg tgcgcatggt gtaacgaatc tcttcgtcga gctgtgccaa ccagtcacga
     9001 aatttccagt catccagcag actggcttcg tgaaagagaa actggctaat gcggtgatgt
     9061 aactccagtg aaacttgcgc actcatttca tcacctcctg ctggtaagcg gcggttttat
     9121 cgagcacttc ctgccagctt tcgctactca gaagatcggc ccagcgttgg tacattccac
     9181 gagcggcagt ttctgaaaag atatagttag taatgccagg aatgccgtcg tcgcggcgct
     9241 tttcctgacc aagccccatt tccagacaca gtttgctgtt gcgggcgcgg tggcctttaa
     9301 gcaatttctg gatttcacac cagttctccg agtcatcctg ctcgagaaaa ccagcaggac
     9361 caaaagcacg agtggcgctg ttttcaaaag cggctttaac ttcatcggag gcggctttgt
     9421 cagtaataca gaacgcccac acttcaactt gatcagggcc gcgcggatgc cagacgcgga
     9481 gtgtggcagt gccgtttagc catgaaagcg tggggaatat attgttatga cccgccaggc
     9541 gcagggcgcg aacttcacct aaacgttgtt ctgcttcggc ataggtttcg cggtaatagc
     9601 ttgaaactgc gccatcgacc cagacattag catccggttt ttcagtaaag aagaaaccgc
     9661 taccgtgacc gtcctgacca aattgcagcg catctttggc ggtttcccac accgggcggg
     9721 cggtttgtcc atcaccgagg cgcttatcgc tgccatcatc tttcgcccct aatacctgaa
     9781 cggcagaagc atggctgaac agagcatgat actggtcact ggcgaactgc tctgccggga
     9841 atttccagtt acagttgatc acccactttt gtacgccgcc gacaatttcg gtgccgcctt
     9901 cgcgacgatc cagcatgcca tccagatacc aggcaatgtc acccaggtaa tcatgcaggc
     9961 ccggtgcgct ggtatcccag ttgccaaaaa tcagtccttt ataactctcc acacaaggaa
    10021 cttcgtttag cccccagtgg gatttacaca acccttgtgg gtaggcgcga ggttccagcg
    10081 gtacatcgat caactcgccg ttaatgccat aagaccagcc gtgatacggg caggtaaagg
    10141 cgcgagtgtt gccacaatct gcataactca cacgcatggc ccggtggcgg cattggttaa
    10201 gaaacgcctt gatgctgccg tctttctggc gcaccacgac aactgcatct tctcccatgt
    10261 aggtgttaaa gaaatcaccg ggttttggga tctggctttc gtgggcgaga aatagccagc
    10321 aacgaccgaa aatacgctca agctccagtt ggtaaatgtc cgggtcggta taaatacgcg
    10381 gagtgacccg accgttttgg gtgtcaatca gttggtaaat gttcaaatct gagggtgtgg
    10441 tcatattttt taatcctgaa agataagatg tattgacctt ctatttttgt gccataagga
    10501 tgttgctggt tggttgctaa tatgtgaaat agttaatttc taccagctga gacgtttttt
    10561 cgataacaag gggaaggtga tggaactacg gcatttacgc tatttcgtcg cagtggcgca
    10621 ggcactgaac tttacccgtg cggcggaaaa actgcatacc tcacagcctt cgttaagcag
    10681 ccagatccgc gatcttgaaa actgtgtcgg tgttccgtta ctggtgaggg ataagcgcaa
    10741 agtcgcgttg acggcggcgg gagaatgttt tctccaggac gcgctggcaa tcctcgaaca
    10801 agcggaaaat gccaaattac gggcgcggaa aattgttcag gaagacagac aattaaccat
    10861 tggctttgtg ccatcggcgg aagtgaattt attgccaaaa gtattaccga tgtttcgtct
    10921 cagacagcca gacaccttga ttgagctggt gagtttaatc accacgcaac aggaggaaaa
    10981 aattcgccgt ggtgaactcg acgtcggctt aatgcgccat cctgtttata gcccggagat
    11041 tgattatctg gagctttttg acgaaccatt agtggtggtg ttaccggttg atcacccttt
    11101 agcacatgaa aaagagatca ccgccgcgca actggatggc gtgaatttcg tcagtaccga
    11161 tccggcgtat tccggttcgc ttgcgccgat cgttaaagcg tggtttgcgc aagaaaacag
    11221 ccagccaaat atcgtccagg tggcaacgaa tattctggtg accatgaatc tggtgggcat
    11281 ggggctgggc gtcactttga tacccggtta tatgaataat tttaataccg ggcaggttgt
    11341 ttttcgtccc attgccggaa atgtgccttc cattgcttta ctgatggcgt ggaagaaagg
    11401 ggagatgaag ccggcattac gcgatttcat cgccattgtg caggaacgtt tggcaagcgt
    11461 aacggcataa ttacgcatta tttttgcaac atgcgccgcg gatcgcagtt ttttagtgct
    11521 tgtgaactgg cgttacagac atcgggaagc aggctcgaat attcttattt ttcaacttgc
    11581 accaacattc gattaacccc agactaacgc ctcctgacgg gagggactca tggttttgca
    11641 atccacgcgc tggttggcgc tcggctattt cacatacttt tttagttacg gcattttttt
    11701 acctttctgg agcgtctggc ttaaagggat tggtttaacg ccagaaacca tcggcctgtt
    11761 attgggggca ggtctggttg cccgttttct cgggagtttg ctcatcgcgc cccgcgtcag
    11821 cgatccttcc cgcctgattt ccgccttgcg cgtgctggca ctgctgccac ttctctttgc
    11881 tgtcgccttc tgggcggggg cgcacgtagc gtggctgatg ctggtgatga ttggctttaa
    11941 cctctttttc tcaccgctgg taccgttgac cgatgcactg gcgaatacgt ggcaaaagca
    12001 gttcccgctt gattacggca aagtgcgact gtggggctcg gtggcgtttg tcattggctc
    12061 ggcgctgacg ggcaaactgg tcactatgtt tgattatcgg gtgatcctcg tgctgttgac
    12121 gttgggcgtg gcatccatgc tgctcggctt tctcatccgt ccgacgattc agccacaagg
    12181 ggcaagccgc cagcaggaga gcaccggttg gtctgcgtgg ttggcgctgg ttcgccagaa
    12241 ctggcgcttt ctggcctgcg tttgtttatt gcagggggca catgcggcct attacggttt
    12301 tagcgccatt tactggcagg cagctggcta ctcggcctcg gcggtggggt atttgtggtc
    12361 gctgggcgtg gtggcggaag tcattatctt tgcgctgagt aataaacttt tccgccgttg
    12421 tagtgctcgc gatatgctgt tgatctcggc gatttgcggc gtagtgcgct ggggcattat
    12481 gggagcaact acggcgttgc cgtggttgat agtggtgcaa attctgcatt gcggcacctt
    12541 cacggtctgc cacctggccg ccatgcgtta tattgctgct cgccaggtta gcgaagtcat
    12601 ccgtttacag gcggtttact ctgccgtcgc gatgggcggc agtatcgcta tcatgaccgt
    12661 tttcgccggt ttcctgtatc aatatctggg ccacggtgtg ttctgggtaa tggcgctggt
    12721 ggcgcttccg gcaatgtttt tgcgcccgaa agttgttccc tcatgctgat tcgagcattt
    12781 tgcggatctg ctcctgttga ggtgttgtca gcgtcaggtc cgtatagatc agcggtggtg
    12841 aaaagagcgg taacggcatg gtataaggcg caataatcag tgccgcgcca cgcggagcgc
    12901 ctgtctgcaa aaatgccttt accgacatat gcttaatgtt gagcggcagt aacgttagtt
    12961 cgcgtaactg ctgctcaatc tgcgcttctc gctcgctatc attcccggtc agtagaataa
    13021 tctgtttttc atgcagatcg ttttcctgca ttagccaggc accgaaaatc accgcgacca
    13081 gaccactttc ctcatcagaa agatggacgc cgtattcact ttcaaatccg gccagcgccg
    13141 cgcgggtgat gcgtaccagg cgtggataca gtctggcgaa ctcttccggc agggtattat
    13201 caataccgat ggcaaacaaa ctgcgattta acgcctgggc gaggtggata taaagctgat
    13261 cacataaccc ctgttcatca taaaaacgaa catttcccag ctcacgaaaa tggtttacca
    13321 gacgtttgat agactgacgc aattgtctgt cccgctgatg cgcatcccgc agtggatcgg
    13381 gaacccgcaa catggaaaaa agtaatgcca taaacagtgg ctcatcaggc ggtacatgct
    13441 ggagcgcccg acgctgccag tggcgtccaa tttcctgcgc tacctgaaat tcaaggcagg
    13501 attccgccca gcgacgttgg agcggattaa actgaggcgt aattccagcg tggtgctgca
    13561 gcaaacaata ttgcagatac agacacagga aatgaatatc gcgcgattca aagcgttttt
    13621 gcagccgtcg ggagcagaga ttcactaacg cttgtagatt ggtatcgtcg taaaagttac
    13681 gcgcaattcc gcgccgcttc agttcactct tcagggcggg ggtgaattgg ctggtaataa
    13741 atgacggaca cagacggaaa ccacgtcgta gccagtgaaa taaacacaaa cgttggttaa
    13801 gcactgtacc ttcaacccgg tagctaccgt cataaccagt cgtcagtgtg agttgatgat
    13861 agcgcaggat ctcccgtcct gtttcgctga tatcaagact ggcaatatca tcatccacac
    13921 cattaagctc gctgaaggtt gccatggtgg cggtcaaccc cggctgaaag agcgtcagca
    13981 agatctggca gcggcgctgg ggagccgaaa ggacagatgg tggagcaagc gtaggcatca
    14041 tctgtagatc tcccataagc ataattgata agaatagcta aagtttgctc agggaaatgg
    14101 cgaagggaat gctaatcatc agaaatgttg gcaagcgtca cagaattttt ctcattttaa
    14161 actgttctga ccagtggatt agcatgtctt aaggtggttt tgtgaatgat tatcgtgcta
    14221 gcgatcatgg gttagttctg cggattcaac gcgctaagga taaagtccac catctgttcg
    14281 cgataaacat tgccgtgacg atcggaaaaa gcatcaatgt gttcgccatc cgggattagg
    14341 atcaaccgtt ttggctcttt tgccaggcta tacaactttt cgctgtgctg ccatgggata
    14401 acgtgatcgg ctttaccgtg aatgagtaaa agcgggatcg ggctgacgct ggcgatgtaa
    14461 ttttcgccgc tgtaactctc atcaagtaag tagccactgc cggggagcat ctggttggcg
    14521 atggttgcat aagaggcaaa tgtggagtcg aggatcaccg cacgtatgcc ttcacgatca
    14581 ccctgaccaa taacagcaag aatattcgcc ccgccaatac tctgcccgaa cagcaccagc
    14641 cgttgtgggt ttacatcact gcgatggcgc accacattga tggcgctttg cgtatcgtcc
    14701 agcaatccgg cctgggacgg agtgcctttt gatttaccaa acccgcgata atcaaacata
    14761 aaaacgttga aattacgctc aggtaaccaa ctgaccagcg gccagtgggc ggacatattt
    14821 ccggcattgc cgtgagcatg aatgatggtt gcgatggcgt tgtcagcagg gcccgtcgaa
    14881 gaaggaataa accagccttg cagacgagta ccgtccttag ccgtaaattc gacggattcc
    14941 gccgaccagg ggtcgggacc gtaaatttta tcgtcaggat aatagaagaa gttgatggca
    15001 acgcgcagca ctaaataaac gcagaaggca acaacaaaga gaataaacag aattttggga
    15061 acgcgtttgt tcactggcag tgccataacc tttccttgcg acttctttat catccattaa
    15121 tcggggaaag atggtgggag agtgacgaaa gaaagtaaag cggtgaggaa gtatttcgga
    15181 atagcgcctg aaaggcgagg gcgggtgagt gatatcaccc gcctgagtca ttaacgcttc
    15241 agagcgtcgc ttaactcgtc acgcatgttc gccagtatgg ctttcacaac acgcgggtta
    15301 ccagcaacga tgttaccggt cagcatatag ttatgaccac cggtgaagtc gctgacgatg
    15361 ccgcccgctt cacgaaccag cagatcgcct gccgcgaaat cccacggacg cagaccgatt
    15421 tcaaagaaac cgtcaacacg acccgcagcg acgtaagcca gatccagcgc cgcagaaccg
    15481 gtacgacgga agtctgcaca ttcgttgaac agcttgccga cgatgttgat gtaggtagtg
    15541 gcgtactgtt ttgctttgaa cgggaagccg gtcgccagaa tggtaccgtc gagatcgcga
    15601 gcggtgctac cgcgcagacg gtagccgttc agctgtgcgc cctgaccgcg agtggcggtg
    15661 aacagttcgt tacgcatagg atcgtatacc acagcaactt cggtgcggcc tttgatacga
    15721 acggcgatag ataccgcgaa gtgcggcaga cgtttgataa agttggtagt accatccagt
    15781 ggatcgataa cccattgaac atcctgatca gtaccttcaa gttcaccgct ttcttcggtg
    15841 atgatggtgt gctgtgggta agatttacga atcgtgtcga taatcaccgc ttcggcagct
    15901 ttatctacgt tggtcacgaa atcgttactg cctttctggc tcgcttctac agcgtccggg
    15961 gtttcatagt ttttggcaat taaattaccc gccttgcgcg ctgcgcgcac ggcgatgttc
    16021 agcatcggat gcatcagtct ctctcactgg atgttaaaga acgggaaaac ggcgcagagt
    16081 atagcagcga gatcggcatc tgtcttcgtt ttatgataag atgcgtgaat aatctttaga
    16141 tgaagaaaga caatgctgca aaatattcga attgtgctgg tggagacgtc acacaccggc
    16201 aatatgggtt ctgttgcccg tgccatgaaa acaatgggat taaccaatct gtggctggtt
    16261 aatccactgg tgaaacccga ctcccaggcg attgccctgg ctgcaggggc cagcgatgtg
    16321 attggtaatg ctcacatcgt cgatacgctc gacgaagcgt tagccggttg tagcctggtg
    16381 gtgggcacca gcgcacgctc ccgcacgctg ccgtggccga tgctcgaccc gcgcgaatgc
    16441 ggtctgaaaa gcgtcgctga agcggcaaat accccggtgg cgctggtgtt tggtcgtgag
    16501 cgcgtcggct tgaccaatga agagttgcag aaatgccatt atcatgtcgc gattgcggca
    16561 aacccggaat acagttcgct gaacctggca atggcggttc aggttattgc ctatgaagtg
    16621 cgtatggcct ggctggcaac tcaggaaaat ggcgagcagg tcgagcatga agagacgccg
    16681 tatccgctgg tcgatgatct ggagcgtttt tatggtcatc tggaacaaac gctgctggca
    16741 accggtttta tccgtgaaaa ccatccgggg caggtgatga ataaattgcg ccgtctgttt
    16801 acccgtgcgc gcccggaaag ccaggaattg aatatcctgc gcgggattct ggcttctatt
    16861 gagcagcaga ataaaggtaa caaggccgaa taacagtggt tgcctgatgc gacgcttaac
    16921 gcgtcttatc aggcctacaa tgtacagaat cgtaggtcgg ataaggtgtt cacaccgcat
    16981 ccgacaacag gtacaaacgc cacgataaaa aaatggcact gaaggttaaa tacccgacta
    17041 aatcagtcaa gtaaatagtt gaccaattta ctcgggaatg tcagacttga ccctgctatg
    17101 caataccccc acttttacaa taaaaaaccc cgggcagggg cgagtttgag gtgaagtaag
    17161 acatgagact gacatctaaa gggcgctatg ccgtgaccgc aatgcttgac gttgcgctca
    17221 actctgaagc gggcccggta ccgttggctg atatttccga acgtcaggga atttcccttt
    17281 cttatctgga acaactgttt tcccgtctgc gtaaaaatgg tctggtttcc agcgtacgtg
    17341 gaccaggcgg tggttatctg ttaggcaaag atgccagcag catcgccgtt ggcgaagtga
    17401 ttagcgccgt tgacgaatct gtagatgcca cccgttgtca gggtaaaggc ggctgccagg
    17461 gcggcgataa atgcctgacc cacgcgctgt ggcgtgattt gagcgaccgt ctcaccggtt
    17521 ttctcaacaa cattacttta ggcgaactgg ttaataacca ggaagtgctg gatgtgtctg
    17581 gtcgtcagca tactcacgac gcgccacgca cccgcacaca agacgcgatc gacgttaagt
    17641 tacgcgctta ataaaaagaa ttcagaatca ggccggagtg ctaaatactc cgtaaacacg
    17701 gtcgtacatc cagccggtag cctgattcct tgcattgagt gatgtacgga gtttatagag
    17761 caatgaaatt accgatttat ctcgactact ccgcaaccac gccggtggac ccgcgtgttg
    17821 ccgagaaaat gatgcagttc atgacgatgg acggaacctt tggtaacccg gcctctcgtt
    17881 ctcaccgttt cggctggcag gctgaagaag cggtagatat cgcccgtaat cagattgccg
    17941 atctggtcgg cgctgatccg cgtgaaatcg tctttacctc tggtgcaacc gaatctgaca
    18001 acctggcgat caaaggtgca gccaactttt atcagaaaaa aggcaagcac atcatcacca
    18061 gcaaaaccga acacaaagcg gtactggata cttgccgtca gctggagcgc gaaggtttcg
    18121 aagtcaccta cctggcaccg cagcgtaacg gtattatcga cctgaaagaa ctcgaagcgg
    18181 cgatgcgtga cgacaccatc cttgtttcca tcatgcacgt aaataacgaa atcggcgtgg
    18241 tgcaggatat cgcggctatc ggcgaaatgt gccgtgctcg tggcattatc tatcacgttg
    18301 atgcaaccca gagcgtgggc aaactgccaa tcgacctgag ccagttgaaa gttgacctga
    18361 tgtctttctc cggtcacaaa atctatggcc cgaaaggtat cggtgcgctg tatgtgcgtc
    18421 gtaaaccgcg tgtacgcatc gaagcgcaaa tgcacggcgg cggtcacgag cgcggtatgc
    18481 gttccggcac tctgcctgtt caccagatcg tcggaatggg cgaagcctat cgcatcgcaa
    18541 aagaagagat ggcgaccgag atggaacgtc tgcgcggcct gcgtaaccgt ctgtggaacg
    18601 gcatcaaaga tatcgaagaa gtttacctga acggtgacct ggaacacggt gcgccgaaca
    18661 ttctcaacgt cagcttcaac tacgttgaag gtgagtcgct gattatggcg ctgaaagacc
    18721 tcgcagtttc ttcaggttcc gcctgtacgt cagcaagcct cgaaccgtcc tacgtgctgc
    18781 gcgcgctggg gctgaacgac gagctggcac atagctctat ccgtttctct ttaggtcgtt
    18841 ttactactga agaagagatc gactacacca tcgagttagt tcgtaaatcc atcggtcgtc
    18901 tgcgtgacct ttctccgctg tgggaaatgt acaagcaggg cgtggatctg aacagcatcg
    18961 aatgggctca tcattaatcg gtatcggaat caggagaatt tataatggct tacagcgaaa
    19021 aagttatcga ccattacgag aatccgcgta acgtgggttc ctttgataac aacgacgaga
    19081 acgtcggcag cggcatggtg ggtgcaccgg cctgtggcga cgtaatgaag ttgcagatta
    19141 aagtcaacga tgaaggtatc attgaagacg cgcgttttaa aacttacggc tgcggttccg
    19201 ctatcgcttc cagctccctg gtcaccgaat gggtgaaagg gaagtctctc gacgaagcgc
    19261 aggcgatcaa aaacaccgat attgctgaag aacttgaact gccgccggtg aaaattcact
    19321 gttctattct ggcagaagac gcgatcaaag ccgccattgc ggactataaa agcaaacgtg
    19381 aagcaaaata agagttgagg tttggttatg tcgattacac tgagcgacag tgcagcagcg
    19441 cgagtaaata cctttctggc taaccgcggc aaagggtttg gcctgcgtct gggcgtgaga
    19501 acctccggat gttcaggtat ggcttatgta ctggaatttg ttgacgaacc gacgccggaa
    19561 gacatcgtgt ttgaagacaa aggcgtgaaa gtcgtggtcg atggcaaaag cctgcaattt
    19621 ctggacggta cgcagctgga cttcgtaaaa gaaggcctga atgaagggtt taaattcacc
    19681 aacccgaacg ttaaagatga gtgtggttgc ggcgaaagct tccacgtttg atgcgcatac
    19741 cgataacccc accgtggtcg cctgcgcgtg gggtttgttt tacctgattc gccgttatcg
    19801 cggcggatcg cagccctgag aatgttatgg attacttcac cctctttggc ttgcctgccc
    19861 gttatcaact cgatacccag gcactgagcc tgcgttttca ggatctacaa cgtcagtatc
    19921 atcctgataa attcgccagc ggaagcctgg cggaacaact cgccgccgta cagcaatctg
    19981 caaccattaa ccaggcctgg caaacgctgc gtcatccgtt aatgcgcgcg gaatatttgc
    20041 tttctttaca cggttttgat ctcgccagcg agcagcatac tgtgcgcgac accgcgttcc
    20101 tgatggaaca gttggagctg cgcgaagagc tggacgagat cgaacaggcg aaagatgaag
    20161 cgcggctgga aagctttatc aaacgtgtga aaaagatgtt tgatacccgc catcagttga
    20221 tggttgaaca gttagacaac gaggcgtggg acgcggcggc ggatacggtg cgtaagctgc
    20281 gttttctcga taaactgcgg agcagtgccg aacaactcga agaaaaactg ctcgattttt
    20341 aatttctgga agctaaacat ggccttatta caaattagtg aacctggttt gagtgctgcg
    20401 ccgcatcagc gtcgtctggc ggctggtatt gacctgggca caaccaactc gctggtggcg
    20461 acagtgcgca gcggtcaggc cgaaacgtta gccgaccatg aaggccgtca cctgctgcca
    20521 tctgttgttc actatcaaca gcaagggcat tcggtgggtt atgacgcgcg tactaacgca
    20581 gcgcttgaca ccgccaacac cattagttct gttaaacgcc tgatggggcg ctcgctggct
    20641 gatatccagc aacgctatcc gcatctgcct tatcaattcc aggccagcga aaacggcctg
    20701 ccgatgattg aaacggcggc ggggctactg aacccggtgc gcgtttctgc ggacatcctc
    20761 aaagcactgg cggcgcgggc aactgaagcc ctggcaggcg agctggatgg tgtagttatc
    20821 accgttccgg cgtactttga cgatgcccag cgtcagggca ccaaagacgc ggcgcgtctg
    20881 gcggggttgc atgttctgcg cttacttaac gaaccgaccg ctgcggctat cgcctacggg
    20941 ctggattccg gtcaggaagg cgtgatcgct gtttatgacc tcggtggcgg gacgtttgat
    21001 atttccattc tgcgcttaag tcgcggcgtg tttgaagtgc tggcaaccgg cggtgattcc
    21061 gcgctcggcg gcgatgattt cgaccatctg ctggcggatt acattcgcga gcaggcggac
    21121 attcctgatc gtagcgataa ccgcgttcag cgtgaactgc tggatgccgc cattgcagcc
    21181 aaaatcgcgc tgagcgatgc ggactccgtg accgttaacg ttgcgggctg gcagggcgaa
    21241 atcagccgtg aacaattcaa tgaactgatc gcgccactgg taaaacgaac cttactggct
    21301 tgtcgtcgcg cgctgaaaga cgcgggtgta gaagctgacg aagtgctgga agtggtgatg
    21361 gtgggcggtt ctactcgcgt gccgctggtg cgtgaacggg taggcgaatt tttcggtcgt
    21421 ccaccgctga cttccatcga cccggataaa gtcgtcgcta ttggcgcggc gattcaggcg
    21481 gatattctgg tgggtaacaa gccagacagc gaaatgctgc tgcttgatgt gatcccactg
    21541 tcgctgggcc tcgaaacgat gggcggcctg gtggagaaag tgattccgcg taataccact
    21601 attccggtgg cccgcgctca ggatttcacc acctttaaag atggtcagac ggcgatgtct
    21661 atccatgtaa tgcagggtga gcgcgaactg gtgcaggact gccgctcact ggcgcgtttt
    21721 gcgctgcgtg gtattccggc gctaccggct ggcggtgcgc atattcgcgt gacgttccag
    21781 gtcgatgccg acggtctttt gagcgtgacg gcgatggaga aatccaccgg cgttgaggcg
    21841 tctattcagg tcaaaccgtc ttacggtctg accgatagcg aaatcgcttc gatgatcaaa
    21901 gactcaatga gctatgccga gcaggacgta aaagcccgta tgctggcaga acaaaaagta
    21961 gaagcggcgc gtgtgctgga aagtctgcac ggcgcgctgg ctgctgatgc cgcgctgtta
    22021 agcgccgcag agcgtcaggt cattgacaat gctgccgctc acctgagtga agtggcgcag
    22081 ggcgatgatg ttgacgccat cgaacaagcg attaaaaacg tagacaaaca aacccaggat
    22141 ttcgccgctc gccgcatgga ccagtcggtt cgtcgtgcgc tgaaaggcca ttccgtggac
    22201 gaggtttaat atgccaaaga ttgttatttt gcctcatcag gacctctgcc ctgatggcgc
    22261 tgttctggaa gctaatagtg gtgaaaccat tctcgacgca gcgctgcgta acggtatcga
    22321 gattgaacac gcctgtgaaa aatcctgtgc ttgcaccacc tgccactgca tcgttcgtga
    22381 aggttttgac tcactgccgg aaagctcaga gcaggaagac gacatgctgg acaaagcctg
    22441 gggactggag ccggaaagcc gtttaagctg ccaggcgcgc gtcaccgacg aagatttagt
    22501 ggtcgaaatc ccgcgttaca ctatcaacca tgcgcgtgag cattaacaga ggttagtatg
    22561 ggacttaagt ggaccgatag ccgcgaaatt ggcgaagcac tgtacgatgc gtatcccgat
    22621 cttgatccga aaacggttcg attcaccgat atgcatcagt ggatttgcga tctggaagat
    22681 ttcgacgacg acccgcaggc atccaacgag aaaatcctcg aagcgatttt gttagtctgg
    22741 ctggacgaag ccgagtgaat gaacgggctg cctgggggta gcccgttttg ctgttaaacg
    22801 aataaggata actaaaatga cagaagcgat gaagattacc ctctctaccc aacctgccga
    22861 cgcgcgctgg ggagaaaaag caacttacag cattaataat gacggcatta ccctgcattt
    22921 gaacggggca gacgatctgg ggctgatcca gcgtgcggcc cgcaagattg acggtctggg
    22981 catcaagcat gttcagttaa gcggtgaagg ctgggatgcg gatcgctgct gggcattctg
    23041 gcaaggttat aaaggcccga aaggcacgcg taaagtggag tggccggatc tggacgatgc
    23101 ccagcgccag gaactggata accgcctgat gatcatcgac tgggtgcgtg acaccatcaa
    23161 cgcaccggca gaagaattgg gaccatcgca actggcacag cgtgctgttg atctgatcag
    23221 caacgtcgcg ggcgatcgtg tgacttatcg gatcaccaaa ggcgaagatc tgcgtgagca
    23281 aggttatatg gggctgcaca ctgtcgggcg cggttcagaa cgttctccgg tattgctggc
    23341 gctggattac aacccaactg gcgataaaga agcaccggtg tacgcgtgcc tggtaggtaa
    23401 aggtatcact tttgactccg gcggctacag catcaaacag actgcgttta tggactcgat
    23461 gaagtcggac atgggcggcg cggcaacggt taccggggcg ctggcatttg ccattacgcg
    23521 cggactgaac aagcgcgtga agctgttcct ctgctgtgcg gataacctga ttagcggcaa
    23581 tgcgttcaag ctgggcgata tcatcactta tcgcaacggt aaaaaagttg aagtgatgaa
    23641 cactgatgcc gaagggcgtc tggtgcttgc cgatggtctg attgatgcca gtgcgcagaa
    23701 accggaaatg atcattgatg cggcgaccct caccggggcg gcgaaaactg cgctgggtaa
    23761 tgattatcac gcgctgttca gttttgacga tgcgcttgcc ggtcgtttac tggcgagtgc
    23821 ctcacaagag aacgaaccat tctggcgtct gccgctggcg gaattccacc gcagccagct
    23881 gccgtctaac tttgccgaac tgaacaatac cggaagcgcg gcgtatccgg caggcgcgag
    23941 cacggcggcg ggcttcctgt cgcactttgt tgagaactat cagcaaggct ggctgcatat
    24001 cgactgctcg gcgacttacc gtaaagcgcc ggttgaacag tggtctgcgg gcgctacggg
    24061 acttggtgtg cgcacgatag ctaatctgtt aacggcgtaa actaatatgc cggatgcggc
    24121 gtaaacgcct tatccggcct acaaattctg caaattcaac aaattgcgaa tcccttgtag
    24181 gcctgataag cgtagcgcat caggcatttg cgccccgaac gttgaatgaa ttgtggttct
    24241 tatgtccgaa acaaaaaacg aacttgaaga cctgctggaa aaagcagcaa ccgaaccggc
    24301 gcaccgcccg gcctttttcc gtactctact ggaatccacc gtctgggtgc ctggtacggc
    24361 ggcgcagggc gaggctgtgg ttgaagattg tgcgcttgat ttacagcact gggaaaaaga
    24421 agacggcacc agcgtcattc cttttttcac ctcgttagaa gcgcttcaac aggcggttga
    24481 agacgaacag gcatttgtcg taatgcccgt tcgcacattg tttgagatga cccttggcga
    24541 aacgctcttc cttaatgcca aactgccgac cggtaaagaa tttatgccgc gtgaaatcag
    24601 tttgttgatt ggtgaagagg gaaatccgct gagcagccag gaaatcctgg aaggcggtga
    24661 atcgctgata ttatcggaag tcgcagagcc gccagcacaa atgattgatt cactcaccac
    24721 cttatttaaa accattaagc cggtgaagcg tgcttttatt tgttcaatta aagagaacga
    24781 agaggcacag cctaatttac ttattggcat tgaagccgat ggtgatatcg aagaaattat
    24841 tcaggcgacg ggaagtgtag cgaccgatac attacctggc gatgaaccaa tcgatatttg
    24901 tcaggtgaaa aaaggggaaa aaggaattag ccactttatt accgaacata ttgcgccatt
    24961 ctatgaacgt cgctggggtg gttttttgcg tgactttaaa caaaaccgga taatctaaaa
    25021 tatgccgccc taaagggcgg cattcgggct tacttgctgc tctgaaggtt gttaaccacc
    25081 agtaaattgt cgccggagag actccggtta ttatttactt gggaaaacag cgcaccagaa
    25141 acaataagta cgatacagaa gcaccgtatc ttcattgtgc tgtcgtgatg cgaagaccgc
    25201 atcacgacag caccactggc gatattgccg cgatagcaag cgctgagacg cgcttgctct
    25261 aacggtcgcg gaacatcctt tccttaagcc agcgcccgct tgcgagacgg cggaaagggc
    25321 cgcagtggat aatacactaa cagaacaata ataactctct tttgcttgac aaaaagagag
    25381 ttactggcga gtattgtctc cgctgagacg cgcttgcatt actacggtaa tacaacctgc
    25441 tggaaaggcc gcgaaccaga ccagcaataa aaaaacgcca aattcggcgg ttttttattg
    25501 cctgtaaaat acttatttca ctggctcaac cggtaaatct gcccgcgcgc cccattcact
    25561 ccatgcgccg tcgtacagtt tcacgtttgg cacatccagc gtcgcgagtg ctaacaaaac
    25621 cacggctgcc gttacaccag agccgcagct gacgataatc ggtttgtcat agctgacgcc
    25681 gcgaccaaaa aatatcgcat ccagttcatc ggtcgttttt agttcgcctt cgcgcaccag
    25741 ttccgtccac ggaacgttca atgctccggg aatatgtccg cgacgtaaac ctgggcgagg
    25801 ttcatcaact tctgcgttaa aacgtgcagc cgggcgggca tcaataattt gcgccgtatt
    25861 ttcatggctt gccaataaca catcggttac tttcaccact gcttcaggat taaacgcggc
    25921 gttaaactct ccttccggca gctctactgc accttcttct aatagcaggt catcgcgctg
    25981 ccagcctgca ag
//
LOCUS       DADLTF010000044        25888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.044, whole genome shotgun sequence.
ACCESSION   DADLTF010000044 DADLTF010000000
VERSION     DADLTF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.044"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            347..640
                     /locus_tag="JN749_001739"
     CDS             347..640
                     /locus_tag="JN749_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368189.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="JN749_001740"
     CDS             712..1371
                     /locus_tag="JN749_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HAY7368190.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1448..1909
                     /locus_tag="JN749_001741"
     CDS             1448..1909
                     /locus_tag="JN749_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HAY7368191.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKDLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JN749_001742"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="JN749_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /gene="umuD"
                     /locus_tag="JN749_001743"
     CDS             3389..3808
                     /gene="umuD"
                     /locus_tag="JN749_001743"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7368192.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQRL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5074
                     /locus_tag="JN749_001744"
                     /pseudo
     CDS             3808..5074
                     /locus_tag="JN749_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
     gene            complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="JN749_001745"
     CDS             complement(5120..5650)
                     /gene="dsbB"
                     /locus_tag="JN749_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HAY7368193.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="JN749_001746"
     CDS             complement(5796..7337)
                     /gene="nhaB"
                     /locus_tag="JN749_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HAY7368194.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7558..8277
                     /gene="fadR"
                     /locus_tag="JN749_001747"
     CDS             7558..8277
                     /gene="fadR"
                     /locus_tag="JN749_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HAY7368195.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8329..9861)
                     /locus_tag="JN749_001748"
     CDS             complement(8329..9861)
                     /locus_tag="JN749_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HAY7368196.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHTCYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10191..11489
                     /locus_tag="JN749_001749"
     CDS             10191..11489
                     /locus_tag="JN749_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HAY7368197.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11499..12569
                     /gene="dadX"
                     /locus_tag="JN749_001750"
     CDS             11499..12569
                     /gene="dadX"
                     /locus_tag="JN749_001750"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HAY7368198.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12737..14163)
                     /locus_tag="JN749_001751"
                     /pseudo
     CDS             complement(12737..14163)
                     /locus_tag="JN749_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109184.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14773..16509)
                     /locus_tag="JN749_001752"
     CDS             complement(14773..16509)
                     /locus_tag="JN749_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HAY7368199.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="JN749_001753"
     CDS             complement(16604..17518)
                     /gene="ldcA"
                     /locus_tag="JN749_001753"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HAY7368200.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17618..18229
                     /gene="emtA"
                     /locus_tag="JN749_001754"
     CDS             17618..18229
                     /gene="emtA"
                     /locus_tag="JN749_001754"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HAY7368201.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18231..18965)
                     /locus_tag="JN749_001755"
     CDS             complement(18231..18965)
                     /locus_tag="JN749_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HAY7368202.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19166..19420
                     /locus_tag="JN749_001756"
     CDS             19166..19420
                     /locus_tag="JN749_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAY7368203.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19470..21424)
                     /locus_tag="JN749_001757"
                     /pseudo
     CDS             complement(19470..21424)
                     /locus_tag="JN749_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(21450..22304)
                     /locus_tag="JN749_001758"
     CDS             complement(21450..22304)
                     /locus_tag="JN749_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HAY7368204.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22304..23113)
                     /locus_tag="JN749_001759"
     CDS             complement(22304..23113)
                     /locus_tag="JN749_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7368205.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23123..23881)
                     /locus_tag="JN749_001760"
     CDS             complement(23123..23881)
                     /locus_tag="JN749_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7368206.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23878..24858)
                     /locus_tag="JN749_001761"
     CDS             complement(23878..24858)
                     /locus_tag="JN749_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAY7368207.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISP
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24858..25880)
                     /locus_tag="JN749_001762"
     CDS             complement(24858..25880)
                     /locus_tag="JN749_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HAY7368208.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6450 a         6372 c         6727 g         6339 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaacggttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta ccggtggttg tgctatcgaa
     3901 taatgacggt tgcgttatcg cccgaaacgc tgaggcaaag gcgctgggcg ttaaaatggg
     3961 cgatccctgg ttcaaacaaa aagatctgtt tcgtcgctgt ggtgtggttt gctttagcag
     4021 caattatgag ctttacgcag acatgagcaa tcgggtgatg tcgacgctgg aagaactatc
     4081 gccccgcgtc gagatttaca gtattgatga agcattctgc gatctgacag gtgtgcgtaa
     4141 ttgtcgcgat ctgactgatt ttggcagaga aattcgcgca acggtgctac aacgtaccca
     4201 tcttactgtt ggtgtgggga tcgcccagac caaaacgttg gctaagcttg ccaatcatgc
     4261 ggcaaaaaaa tggcagcggc agacgggtgg ggtggtggat ttatcaaatc tggaacgcca
     4321 gcgtaaatta atgtctgctc tccccgtgga tgacgtctgg gggattggac ggcggatcag
     4381 caaaaaactg gacgcgatgg ggatcaaaac cgttctcgat ttggcggata cagatatccg
     4441 gtttatccgt aaacatttta atgtcatgct cgaaagaacg gtgcgtgaac tgcgcggcga
     4501 accctgtttg caactggaag agtttgcacc gacgaagcag gaaattatct gttcccgctc
     4561 gtttggtgaa cgcatcacgg attatacgtc gatgcggcag gccatttgta gttacgctgc
     4621 ccgggcggcg gaaaaacttc gcagtgagca tcaatattgt cggtttatct ccacatttat
     4681 taagacgtca ccatttgcgc tcaatgaacc ttattacggc aatagcacgt cggtaaaact
     4741 gctgacgccc actcaggaca gcagggatat cattaacgct gctacgcgat ctctggatgc
     4801 catctggcaa gcgggccatc gctaccaaaa agcgggcgtg atgctggggg atttcttcag
     4861 tcagggagtc gcgcagctca atttattcga tgacaatgca ccgcgccccg ggagtgagca
     4921 attgatggcg gtaatggata ctctgaatgc taaagagggc agaggaacac tctattttgc
     4981 cgggcagggg atccaacagc aatggcagat gaagcgagct atgctttcac cacgttatac
     5041 aacgcgaagt tctgatttac tgcgggtcaa ataaatatag cggcaggaaa aaagcgttcc
     5101 cgcaggagcg ccgaatggat tagcgaccga acagatcacg ttttttcgct ttaaacggct
     5161 gggaaatcac caccagcacc gcgacaatca ggtaagcgat aaaaataccg agcagccact
     5221 gcggcatttc catacctaaa aaatcccact gacgctcggc gcaatcgcca gaggcgacaa
     5281 acacttgcgg cacccattta tccagcggca gccattccgg gaaacgaacc ataaaatcac
     5341 aggtggcaaa tggcgaagga tagagctgaa gcatggtgtg ctcgtaagtt aactgcacac
     5401 cgcggaacgc actatacaac cagataacca tcgctacata acgcagcgga gttttcgggg
     5461 cgatcgcgcc aatcagcgca gcacccagaa cgccgaataa cgcgcagcgt tcataaatac
     5521 agagcacgca aggtttcagt aacatcacat gctggaacca cagcgccgtc agttccagtg
     5581 ccagagcagt aaacgccatc aacagccacg cgccccggcc ttgtgaacat tggttcaaaa
     5641 atcgcaacat aatcatttcc ctgcaatatg catagagtgc gcagtttaaa ccaattcatt
     5701 cgctgcgcca ccaggaggca gcaaaaatag gacgcaattg gacaattatc gggcgaaaag
     5761 gcaaaccggg catttatatg cccggtaagt tgttatcaaa gcgttgctat ccagcccatt
     5821 tgcataaacc attcggttac aggggcaagc gtaaactcga cgcagagcaa gccgacgagt
     5881 gtcagtacga gggtgtaagg cagggccatc cacaccatgc ggccataaga gaggcgaatc
     5941 aatggcgcga gtgcagaggt cagcaggaac aggaacgcag cctgaccgtt cggcgtagcg
     6001 acagagggca gattggtacc ggtattaatg gcgaccgcca gcagctcgta ttgcttcaac
     6061 gtgatagcgc cactttccat tgccgctttc gcttcgttga tataaatcgt ccccacgaag
     6121 acgttatccg aaatggatga cagcagaccg ttgaaaatat agaacagcga cagctgagca
     6181 tgttcagatg cctgcaacac aaactgaata atcggcgaaa acagttgttg gtcgataatc
     6241 accgcgacta ccgagaaaaa caccgtcaac agtgcggtga atggcagaga ttcggtgaag
     6301 gctttaccga tagcatgttc atcggtgaca ccggtcaatg atgttgccag aataatgact
     6361 gacaaaccaa tcaagccaac ttccgccaga tgcaacgcca gcgcagtcac cagccagacg
     6421 ccaataatcg cctggacaat cagacggatt ttatcctgac gggtacgctg gtggcggctt
     6481 tgatcgtcaa actgttgcaa cacttcgcgg actttctccg gcagcgtttc accgtagcca
     6541 aaccaacgca gcttctctac cagcaggcag gttaacaggc cacaaatcag aaccggaacg
     6601 gtcaccggcg acatgcgcag gaagaaatcg ccaaaatgcc agccagccgc tttagcgatg
     6661 atcaggttct gtggttcgcc caccatggtc attacgccgc ctaatgcggt gccgacaccg
     6721 gcatgcatca tcaggctacg cagaaagcca cggaactgtt ccagaaccac gttgtaatgc
     6781 ttgtcgatat gactatcgtc ttgcaggtcg gtgtcttcgg tacgggaaga ggctacgcga
     6841 tgataaatac cataaaaacc gactgcaacg ctgatcacca ccgccacgac ggttaaggca
     6901 tcgaggaacg cggagaggaa cgctgccgcc acgcaaaaag agagcgacag cagcattttg
     6961 gagcgaatgc ttaacagcaa acgggtaaat atgaacagca acagctgttt cataaaatag
     7021 atacccgcca ccataaacat caataacagc aagacttcaa gatttgccgc caccccttca
     7081 cggacgtgtt ccgcgctggt catgccgatg aataccgctt cgatagccaa cagaccaccg
     7141 gggagtagcg ggtagcattt cagggccatc gccagagtga aaataaattc cgctaccagc
     7201 aaccagcccg cgacgaaagg gctgatgagg aaaattaacg ggtttacgat taagaaaatg
     7261 atgagggcga gtttgtacca gtcgggggac tggcccaaaa aattgcgcca tagcgcgcgg
     7321 ccccaggaga tctccatgat ggtttccctt accttacaaa taatcaatga tgtttttgtg
     7381 tttaaacgca aagcttaacg gtcaggcagg agtgaggcaa gtcttgatag tcaaggggaa
     7441 agagatgcgg aaaatgaagc cttgatccct ttttcttctt tttgtctgct atcagcgtag
     7501 ttagccctct ggtatgatga gtccaacttt gttttgctgt gttatggaaa tctcactatg
     7561 gtcattaagg cgcaaagccc ggcgggtttc gcggaagagt acattattga aagtatctgg
     7621 aataaccgct tccctcccgg gactattttg cccgcagaac gtgaactttc agaattaatt
     7681 ggcgtaacgc gtactacgtt acgtgaagtg ttacagcgtc tggcacgaga tggctggttg
     7741 accattcaac atggcaagcc gacgaaggtg aataatttct gggaaacttc cggtttaaat
     7801 atccttgaaa cactggcgcg actggatcac gaaagtgtgc cgcagcttat tgataatttg
     7861 ctgtcggtgc gtaccaatat ttccactatt tttattcgca ccgcgtttcg tcagcatccc
     7921 gataaagcgc aggaagtgct ggctaccgct aatgaagtgg ccgatcacgc cgatgccttt
     7981 gccgagctgg attacaacat attccgcggc ctggcgtttg cttccggcaa cccgatttac
     8041 ggtctgatcc ttaacgggat gaaagggctg tatacgcgca ttggtcgtca ctatttcgcc
     8101 aatccggaag cgcgcagtct ggcgctgggc ttctaccaca aactgtcggc gttgtgcagt
     8161 gaaggcgcgc acgatcaggt gtacgaaaca gtgcgtcgct atgggcatga gagtggcgag
     8221 atttggcacc ggatgcagaa aaatctgccg ggtgatttag ccattcaggg gcgataatcc
     8281 cttccgttta aagagcaaac ccctcaaacg aggggttttt tgttgttttt acagatttcc
     8341 cattcttggc gggcaacgtt ccagcaactc gatgctgccg tcttcgtttt gctgttcgag
     8401 catcacatca aatccccaca ggcgatgcac atgcttcagg acttctttgc gcccccgatc
     8461 cagcggtgcg cgattatgtg gaatataacg cagcgtcagc gaacggtcgc cgcgcaaatc
     8521 cacgttccag atctgaatat tcggctccag attacttaag ttatattgcg acgataaccg
     8581 gttacggatc tcccgataac cttcttcatt atgaatagcg gaaatctcca gataattatg
     8641 ccgatcatca tccagcacgg tgaagaagcg gaaatcacgc atcactttcg gtgacagaaa
     8701 ctggctgata aaactctcat ctttgaaatc acgcatcgcg aaatgcagcg tttccagcca
     8761 gtcggaaccg gcgatatccg ggaaccagta tttgtcttct tccgttggcg actgacaaat
     8821 ccgtttaata tcctggaaca tggcgaaccc gagggcatac gggttgatgc cgctgtacca
     8881 cgggctgtta taggggggct ggaagaccac attggtgtgg ctgtgcaaaa actccagcat
     8941 aaaacgttcc gttactttcc cttcatcata cagatggtta aggatggtat agtgccagaa
     9001 ggtcgcacag ccttcgttca taacctgagt ctgtttttgc ggataaaaat actggctcac
     9061 cttacgcaca atacgcagga tttcacgctg ccatgattcc agcagtggcg catttttctc
     9121 cataaaatag agcaggtttt cttgtggttc ggacggatag cggcgcgctt cagcaaccgt
     9181 tttctcttcc tcgcgcttcg gcagggtacg ccagagcata ttgacctgac tttgtagata
     9241 ctcttcgcga cttttctgcc gggctttctc ttcttgcagc gagatttttt gcgggcgttt
     9301 gtaccggtcc acgccgtagt tcatcagcgc gtggcacgag tccagaagcc gttctacttc
     9361 atcaacgcca taacgttctt cgcactcggt aatatattta cgggcaaaaa tcagataatc
     9421 gacaatcgaa ctggcgtcgg tccaactgcg gaataagcaa ttgtttttaa agaaagagtt
     9481 atgcccatag caggtgtgtg ccatcaccag cgcctgcata gtaatggtgt tctcttccat
     9541 caagtaagcg atacacgggt tagagttaat gacgatttca taggccagcc cttgctgacc
     9601 gtgcttatac agccgttcag tctcgataaa ctttttaccg aatgaccagt gcggatagtt
     9661 aattggcatg ccgacgctgg aataggcatc catcatctgt tctgaggtta tcacttcaat
     9721 ctggtgcggg taagtatcca gccggtagag tttcgccacc cggtctatct ctgccagata
     9781 aacatccagc aggtcgaacg tccagtcggg tccatcgctc aaacgtgtgg tgtccttatt
     9841 aatagaatcg atcgtcgcca tacgcgcacc tcattgttgt cggcgctctc tgtgtggagc
     9901 acctcatttc aagcatagaa cacctgttaa aaaccgcgtc gccggagaat ttttttcttt
     9961 gcgatttctt attatcagag tgccactaat ccgcttctga acggaatttt atgctggata
    10021 aaaagggcgt tcagcaggag atactaaaga cgccatattg ccgcagagtc agggagatgt
    10081 gagtcagctc accataaaaa agccgtatgt tgaataatat tttcaactga gttatcaaga
    10141 tgtaattaga ttattattct tttactgtat ctaccgttat cggagtggct atgcgagttg
    10201 tcatactggg aagtggtgtg gtaggcgttg ccagcgcctg gtacttaaat caggcaggac
    10261 atgaggtcac cgtcattgat cgggagccgg gggccgccct ggaaaccagt gccgcgaatg
    10321 ccgggcaaat ctcccccgga tatgctgcac cgtgggcagc accaggtgtg cctttaaaag
    10381 cgattaaatg gatgttccag cgccatgcgc cgctggcggt tcgtctcgac ggtacgcagt
    10441 ttcagttgaa atggatgtgg caaatgttac gtaactgcga caccagccac tacatggaaa
    10501 acaaagggcg gatggtgcgt ctggcggaat acagccgtga ttgcctgaaa gcattgcgcg
    10561 ccgaaaccaa tattcagtat gaagggcgtc agggtgggac gctgcaactg ttccgtaccg
    10621 aacaacagta tgaaaatgcg acccgcgata tcgccgtgct ggaagatgcc ggcgtaccgt
    10681 atcagctgct ggaatccagc cgcctggcgg aagtggatcc cgcgctggca gaagtagcgc
    10741 acaaactgac gggcggcctg cagttaccca atgatgaaac cggagactgc cagctgttta
    10801 cccagaatct ggcgcggatg gcagagcagg cgggggttaa attccgcttt aatacgcccg
    10861 ttgaccaact gctttgcgac ggcgagcaaa tctacggcgt gaagtttggc gatgaagtga
    10921 ttaaggccga tgcgtatgtg atggcgtttg gttcttactc gacggcgatg ctcaaaggca
    10981 ttgttgatat tccggtgtat ccgctgaaag gctactcgct gactattcca attgcgcagg
    11041 aagatggtgc gccagtatcc accattcttg atgaaaccta caaaatcgcc attacccgtt
    11101 tcgataaccg cattcgtgtt ggcggaatgg cggaaattgt tggttttaac accgagctgt
    11161 tgcaaccgcg tcgtgaaacg ctggaaatgg tggttcgcga tctctatcca cgcggcggtc
    11221 atgtcgagca ggcgactttc tggacgggtt tgcgcccgat gacgccagac ggtacgccgg
    11281 ttgtcgggcg tacacgcttt aaaaatctgt ggctgaatac cggtcacggc acgctcggct
    11341 ggacgatggc ttgcggttcc ggtcagttgt taagcgatct gctctctggt cgcacgccag
    11401 cgatcccata tgaggatcta agcgtagcgc gctacagccg tggatttacg ccatcacgtc
    11461 cgggccattt acatggcgca cacagctaag gaaacgagat gacccgtccg atacaggcca
    11521 gcctcgatct gcaggcatta aaacagaatc tgtccattgt ccgccaggcc gcgccgcacg
    11581 cgcgcgtctg gtcggtggta aaagcgaacg cttacgggca tggtattgag cgtatctgga
    11641 gcgcgctcgg ggccaccgat ggctttgcat tacttaacct ggaagaggca ataacgttac
    11701 gtgagcgcgg ctggaagggg ccgatcctga tgctggaagg atttttccat gctcaggatc
    11761 tggagattta tgaccagcac cgcctgacca cctgcgtaca cagcaactgg cagctcaaag
    11821 cactgcaaaa tgcgcggcta aaagcaccgt tggatattta tcttaaagtg aacagtggga
    11881 tgaatcggtt gggcttccag cccgatcgcg tgcttaccgt ctggcagcag ttgcgggcaa
    11941 tggcgaatgt tggcgaaatg accctgatgt cgcattttgc tgaagcggaa catcctgatg
    12001 gaatttccgg cgcgatggcg cgtattgagc aggcggcgga ggggctggag tgtcggcgtt
    12061 cgttgtccaa ttcggcggcg actctgtggc acccggaagc gcattttgac tgggttcggc
    12121 ctggcattat tttgtatggc gcttcgccgt ccggtcagtg gcgtgatatc gccaataccg
    12181 gattacgtcc ggtgatgacg ctaagcagtg agattattgg tgtccagacg ctaaaagcgg
    12241 gcgagcgtgt gggctacggc ggtcgctata ctgcgcgcga tgaacagcga atcggcattg
    12301 tcgccgcagg atacgccgac ggttatccgc gccatgcgcc taccggtact cctgttttag
    12361 tggacggcgt gcgcaccatg acggtgggga ccgtctcgat ggatatgctg gcggttgatt
    12421 taacgccttg cccgcaggcg gggattggta cgccggttga gctgtggggc aaggagatca
    12481 aaattgatga tgtcgccgcc gctgccggaa cggtgggcta tgagttgatg tgcgcgctgg
    12541 cgttacgcgt cccggttgtg acagtgtaac ttgttgtaag ccggatcgga ggcagcgtct
    12601 tctggttgcg aaaaaatcat ccatccggct ggtcagcaac tgtagttgtt aatgtgacag
    12661 agccattgcc catgatagtg tccattaaaa gaatggacac tatttccccg gaacctgaac
    12721 tcgccgcaca ggcgttctac ataaaacgct tacgcttcat tgttgactcg gagtcgactt
    12781 agataaatca cgcttcaccc ttgatgcaga ttcgggttca tgttcagatt gttgattaac
    12841 aggctgcgag tcctgttgtc tttccagact caacaggccc aactgttgat ccaggagctc
    12901 caggtcctcc tgtaccttca cgcgctccag gagttccggg tcatccagga actccagatc
    12961 cataaggtcc tccagctcct cgagtccttc caggtcaacc tagccttcct tgtgcaaaaa
    13021 gtccaaaagg tccttcaggc cctgctgttt tttcaggccc tcgacggctt tgagcagcgt
    13081 cggtaaattg tctttgtccc acgggttacc attacagtca aacaccttca ggctctgggg
    13141 ctgcggcacc tgtaccgttt taaacttgtt ttctccacag tagtaaatta ttagtttggg
    13201 cggcagccgt ggtgaagaga tcaaatcgtt actagaacag tcaaatactt tcaggcgagt
    13261 caagttctcc agcatctcat caggtattga gttaatattg ttgccggcac aggatagttt
    13321 atacaggtac tggggcatcg acggcggtaa aaattgcaag aagttttcag aacagttaat
    13381 cactctcagg ttagtcgaca gctcaggcag ccagataata cagttttcat agcacttcag
    13441 taccatcagt tttgagggct gcaccttcgg taacaagatc aagacgttat ctgtacagtc
    13501 aagcaccagc agccactttg gcatcacctt cggtaacgcg gtcaacacgt ttttactgca
    13561 gtaaaattca atcagatgtg gaggcaaatt ctcagggagt gaagtcaatc ccaggttgct
    13621 aagattgagc atactccgtc ttgtaatgag acattccttc attctgcgca atgcttctgc
    13681 ccggttttca atccggcttg ctggcaggtt ttcttccagg atattttcct tgatttcttc
    13741 cagactttct ttcaggtctt cttggggaat ttctgactgt ttgttttgct ggttttcttc
    13801 caggatttcg tacaggtttt tttgcaggtt ttcttgcaaa ccatcctgct gtatagccca
    13861 ttcgcaccat ttggcgacca tcatgaactc gttatgttgc tgattcaggc gagtccccaa
    13921 cgtgatgatg gtattgttgt ccacctgtat gttcactttg cccgcgatac tgaggtcaat
    13981 gatctgccag ggggcatcac cccagctgaa tttttgcggg ctgagatact tcatgttctg
    14041 caaaatttga ttcttgagtt ccaccgacag gaagagccgc cctggcgggc gtcgtacgtc
    14101 tgggttaacg gaagatgggg gaatggcggg ggcgaatggg cagatgtctt taaagctagc
    14161 cataatgcta cgtttccttg aaataaatat cctctggcag cgccggagac gtgaatgtgg
    14221 tcaccgcaaa ggagccacag gacgtcattt gcgctgatat cactactcat cgttgacttt
    14281 ttccctggtc agaaatgccg tgtcgggtct ggtacatgct gtattacggc ctaggtagct
    14341 ctgacgtatt aaagaaaata cgagagtgaa cgacccggaa ataatcgtta ttaagcaaag
    14401 gaataactat aagaaatcgc tcatgcatcc ggtgctacag gcggatgaaa cgcctattgt
    14461 gtgggctatg tcagtgcggc aggttgcgtg gggaatatgt tcaggcgatg tcaggcaggg
    14521 gcccctgatg gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa
    14581 agcccttacg ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg
    14641 gtttgtcatc cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa
    14701 atgacaagtt tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat
    14761 gctgcattaa atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac
    14821 ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc
    14881 gacaaccggt gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc
    14941 acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct
    15001 ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct
    15061 gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc
    15121 ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc
    15181 aatacgcgtc tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc
    15241 agcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac
    15301 gcgtgacacc ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga
    15361 gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa
    15421 cagtcgcgca ttctccagcc ccgccatcat cgggaacacg gccaggatga ttggcaccgc
    15481 gccgcgtaat cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg
    15541 gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat
    15601 gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag
    15661 gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg
    15721 gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct
    15781 gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg
    15841 atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc
    15901 aagaccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact
    15961 gacactgctt tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat
    16021 tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt
    16081 accacccagc agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa
    16141 taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga
    16201 ggtgataagt acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga
    16261 actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac
    16321 catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag
    16381 catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact
    16441 aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt
    16501 ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta
    16561 gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag
    16621 gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg gcatgtgcgc
    16681 ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa
    16741 tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat
    16801 cattgggcgt gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc
    16861 ccgcatgata gagctgcaac agcatacgtt cgacgcggaa agggtgctcg ttaatatctt
    16921 ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca
    16981 gcatcgcaag attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt
    17041 gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg
    17101 cgttcagttc atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat
    17161 tgccttgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta
    17221 acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac
    17281 tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa
    17341 gggaattgag atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg
    17401 caataacctc gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca
    17461 gcgcggcgtg ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg
    17521 caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc
    17581 acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt
    17641 tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa
    17701 cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc
    17761 agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa
    17821 tccgaacgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc
    17881 cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa
    17941 aaatccagag cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc
    18001 gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa
    18061 cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat
    18121 caacgatttg gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca
    18181 ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc
    18241 actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc
    18301 cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt
    18361 tcattcttgc catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga
    18421 aaaacacccc attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat
    18481 tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag
    18541 cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt
    18601 ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg
    18661 gtaataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg
    18721 actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg
    18781 ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt
    18841 ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa
    18901 tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga
    18961 ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa
    19021 aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc
    19081 ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc
    19141 gattcatcga aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat
    19201 agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac
    19261 ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat
    19321 tggcggctcc atcagcggtt ttaatttgca cagcttccta gtggcggtgg tgggcgctat
    19381 cctggtactg ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag
    19441 cgatacagat gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa
    19501 ttgcgacctt cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg
    19561 accgacgcat tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc
    19621 gcaaaaccgg cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct
    19681 tccgacagcg ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc
    19741 tgcgttggaa gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga
    19801 gcgtagctca atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga
    19861 ccgctgtaat ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc
    19921 gagaggatgg catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc
    19981 gtgaaggcac cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg
    20041 ttaacaatcg ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc
    20101 ggaaagcgtg tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat
    20161 ttccccatca cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg
    20221 ccatctttct cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc
    20281 acgacatcga cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga
    20341 tcttcatagc gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc
    20401 cagttaacgg caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg
    20461 gcaccgtcgc tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc
    20521 agcgagttgt acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg
    20581 gtaaaacggt cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct
    20641 ggccactgcc agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt
    20701 tcaccatcct gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg
    20761 agcttcacaa tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct
    20821 gcaatatcat tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg
    20881 ctgacttgca aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca
    20941 ttgtcctgac tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga
    21001 tattaatggc tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa
    21061 cttcgactgc ccccagattg ttggtcagaa cccgcgccag atcgaggttg ccgtcatagg
    21121 gaacataaat gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa
    21181 cttttacctg ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac
    21241 ttaatgcctg agcgacattc tgtttatcca aggctttcat ggtgggttga tcgagaacgg
    21301 tggtcgtcgt cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg
    21361 ctacggcctg ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc
    21421 tcatagaata aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact
    21481 ttaatgtcag ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag
    21541 tcgaggtaat ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga
    21601 ggagccagcg acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg
    21661 agctgaagta cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct
    21721 tcaacaacaa ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag
    21781 ccctgattgt catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat
    21841 ccggcacgat gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg
    21901 cccggaattg cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat
    21961 gccagcattt gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact
    22021 gccttccatc cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc
    22081 gcgttcgctt gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac
    22141 atcttacacg cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata
    22201 tagccaggct ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct
    22261 tccagcagta acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg
    22321 gtacgctatc ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat
    22381 cttgttgttg ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac
    22441 tccagctgac gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc
    22501 cacggataaa atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga
    22561 tatagttggg taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga
    22621 acgaggtgct gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc
    22681 tcattgcatg gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc
    22741 gtggcaggtc actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct
    22801 tcagtgcggc ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat
    22861 aaaccgtagt gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat
    22921 cgaataaggt ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt
    22981 ccgttggcga ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg
    23041 ttttttctgt gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat
    23101 catcaatgag catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt
    23161 cggcagaaac ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc
    23221 cttgtgttac tcgtccatct ggctctacct ggataatgct gtcggcaatg gcgttggcat
    23281 ggagcggatg gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg
    23341 tgtccagaag ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca
    23401 acaagcgcgg ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc
    23461 taagcgtatt ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg
    23521 tggctttcag gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc
    23581 ccatcaacac catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc
    23641 aggcaatagc ctgtgcgcgc tggcgatggc tgagtgtctg cacagggagg ctatccagta
    23701 ggatctcgcc ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc
    23761 cgcaaccatt cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt
    23821 cgcgtagcac tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca
    23881 ctggcgacct ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag
    23941 aatgccaagt gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag
    24001 cagaatagcg ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa
    24061 caagcgggca atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac
    24121 cgtgctggcg gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt
    24181 cagtcccagc gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag
    24241 aaaaaacagg ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa
    24301 ggtcacggtg gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg
    24361 atcggccagc gttttaattg gcgatatccc cgctccgcac aacgttccta acgcaatgcc
    24421 taccagcacc agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac
    24481 gcccgcgacc accatcagct cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa
    24541 tccccacagg attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc
    24601 cggtgaaact aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc
    24661 tgcgcccagt aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg
    24721 ttcctgcaca gacacatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag
    24781 atgataagcc ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca
    24841 tccgtttacg attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa
    24901 caatgccaga acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg
    24961 ggatccagcc aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca
    25021 tcgagccagc caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc
    25081 acgccctgcc agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga
    25141 ataatatccg gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga
    25201 tcggctattt gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa
    25261 ccctgtaagc ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg
    25321 gcgggtgagg tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt
    25381 tctgcggcgc ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat
    25441 tgcgccagtt ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt
    25501 gcctgggaga tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac
    25561 gccataagcc cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt
    25621 aactggcgaa tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa
    25681 aaaccgacca ttttttctgg cgcgacagcc agcaatagca aatcggcggg cgcgcctgcg
    25741 ctgacgatgc gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact
    25801 gtttgtgaaa aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac
    25861 gtccgtctgg ttatgggcat ggtgaaca
//
LOCUS       DADLTF010000045        25702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.045, whole genome shotgun sequence.
ACCESSION   DADLTF010000045 DADLTF010000000
VERSION     DADLTF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.045"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..326)
                     /gene="yfdR"
                     /locus_tag="JN749_001763"
     CDS             complement(<1..326)
                     /gene="yfdR"
                     /locus_tag="JN749_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7368209.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPP"
     gene            complement(454..1278)
                     /locus_tag="JN749_001764"
     CDS             complement(454..1278)
                     /locus_tag="JN749_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HAY7368210.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(1344..1724)
                     /locus_tag="JN749_001765"
     CDS             complement(1344..1724)
                     /locus_tag="JN749_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368211.1"
                     /translation="MMRSSKMASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMN
                     TKTKGKVSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTI
                     LQEDQGQLLTLAGEPDGKLRAAGH"
     gene            complement(1721..2031)
                     /locus_tag="JN749_001766"
                     /pseudo
     CDS             complement(1721..2031)
                     /locus_tag="JN749_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2307..2603)
                     /locus_tag="JN749_001767"
     CDS             complement(2307..2603)
                     /locus_tag="JN749_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368212.1"
                     /translation="MLKQVYEMKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIM
                     AGVVFSLRRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            complement(2776..3450)
                     /locus_tag="JN749_001768"
     CDS             complement(2776..3450)
                     /locus_tag="JN749_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HAY7368213.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            3541..3741
                     /locus_tag="JN749_001769"
     CDS             3541..3741
                     /locus_tag="JN749_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7368214.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            3785..4336
                     /locus_tag="JN749_001770"
     CDS             3785..4336
                     /locus_tag="JN749_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368215.1"
                     /translation="MGKHHWKIEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDEQYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            4512..4691
                     /locus_tag="JN749_001771"
     CDS             4512..4691
                     /locus_tag="JN749_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003034732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7368216.1"
                     /translation="MRQVNRWFKEHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            4681..5622
                     /locus_tag="JN749_001772"
     CDS             4681..5622
                     /locus_tag="JN749_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7368217.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            5619..6113
                     /locus_tag="JN749_001773"
     CDS             5619..6113
                     /locus_tag="JN749_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7368218.1"
                     /translation="MSMSLLNDVQKFIEAHRGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            6113..6766
                     /locus_tag="JN749_001774"
     CDS             6113..6766
                     /locus_tag="JN749_001774"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HAY7368219.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVLTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            6763..7089
                     /locus_tag="JN749_001775"
     CDS             6763..7089
                     /locus_tag="JN749_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7368220.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERDLKV"
     gene            7086..7475
                     /locus_tag="JN749_001776"
     CDS             7086..7475
                     /locus_tag="JN749_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7368221.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITINKYEKTAGAAA"
     gene            7495..8292
                     /locus_tag="JN749_001777"
     CDS             7495..8292
                     /locus_tag="JN749_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7368222.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSQDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVSRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            8300..9289
                     /locus_tag="JN749_001778"
     CDS             8300..9289
                     /locus_tag="JN749_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7368223.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            9304..9672
                     /locus_tag="JN749_001779"
     CDS             9304..9672
                     /locus_tag="JN749_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein Q"
                     /protein_id="HAY7368224.1"
                     /translation="MMRDIQMVLERWGAWAASDSSGVDYSPIAAGFKGLLPYTSKKRL
                     ACSDSDALIIEGCLARLKQKRPYEHSLLVAHYLYRISKRKIAKARGKDEKLIRIEIQL
                     AEGFIDGCLSMLDLTLDLDV"
     gene            complement(9701..11032)
                     /locus_tag="JN749_001780"
     CDS             complement(9701..11032)
                     /locus_tag="JN749_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HAY7368225.1"
                     /translation="MLDTLSFTERDEFQRRNIAENIIKLLKPEADISPLVIDGAWGTG
                     KTEFSIKLKNLIIEQETESKVVYIDAFKGDHAESPLLLITSAIASILPEEEKQHFIKR
                     SLPAIRFGLKTVLKAGAGWFLRQEASEVSEEFQDAMKKASNAAIDGTIENLLEDHMES
                     EKNINSLKSCIESISESQKIVIIIDELDRCKPSFSTNVIETIKHIFDINNVFFILVTN
                     TEQLKASINHIYGYSINSQKYLDKFIKYTITLPDTCLINGHVVCKTSVIYWNYLVDKT
                     KSLNEINRLSGRFIRDLIQRTNLSLRETQTFSRNLNIFQLLNDDEYKSSDPLINKIFV
                     VAVFIHCFGDKEKLKQEITAESISHLADLLNIKEIPYSYERRSQIPEISIVFFGIIKD
                     SITLNERFAPKSDEELKKFTTVYTDYEHLNFWSTTPRELLIKYINQMSFIQ"
     gene            11329..11655
                     /locus_tag="JN749_001781"
     CDS             11329..11655
                     /locus_tag="JN749_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY7368226.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            11659..12135
                     /locus_tag="JN749_001782"
     CDS             11659..12135
                     /locus_tag="JN749_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY7368227.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLALKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKEAGGVVNEAEL"
     gene            12119..12511
                     /locus_tag="JN749_001783"
     CDS             12119..12511
                     /locus_tag="JN749_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HAY7368228.1"
                     /translation="MKLSYKLLIVGFFVTVIGSFIWSANHYYSKYQYEKKRADEAVRN
                     AESATAITNNVLQSLQIVNTVLEANQHAKQQIALESQRAREDIKVAVADDDCASRPVP
                     DVAADRLRKYADSIRAGSNDAVTDEPAR"
     gene            12974..13306
                     /locus_tag="JN749_001784"
     CDS             12974..13306
                     /locus_tag="JN749_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368229.1"
                     /translation="MPVNIDPEKLNDEREQVIAKWLFKDVDLISQQIELGEENVKRFD
                     ELLSIFDCCQSSWFATEHLFDNTELEKVWHEFESNFNKYINGGESKDLLMKMLDKLIS
                     SRFVFESR"
     gene            13357..13707
                     /locus_tag="JN749_001785"
     CDS             13357..13707
                     /locus_tag="JN749_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7368230.1"
                     /translation="MPPRIPKACRVRGCRSTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDVIRVRVLKRDKGLCQLCLRAGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKEKTARERLK"
     gene            13833..14327
                     /locus_tag="JN749_001786"
     CDS             13833..14327
                     /locus_tag="JN749_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003832359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7368231.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEELPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIARQG
                     NTITGAMGGMVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            14561..16057
                     /locus_tag="JN749_001787"
                     /pseudo
     CDS             14561..16057
                     /locus_tag="JN749_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005147477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
     gene            16069..16251
                     /locus_tag="JN749_001788"
     CDS             16069..16251
                     /locus_tag="JN749_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368232.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQSSVGGGK"
     gene            16251..17492
                     /locus_tag="JN749_001789"
     CDS             16251..17492
                     /locus_tag="JN749_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7368233.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADDIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPSCVVPKLNSSWEPVYQVTFPDGSADVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            17470..18120
                     /locus_tag="JN749_001790"
     CDS             17470..18120
                     /locus_tag="JN749_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HAY7368234.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYIALSLRDAEDVGSALNALKNLNF"
     gene            18135..19340
                     /locus_tag="JN749_001791"
     CDS             18135..19340
                     /locus_tag="JN749_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7368235.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            19390..19590
                     /locus_tag="JN749_001792"
     CDS             19390..19590
                     /locus_tag="JN749_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368236.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            19593..19916
                     /locus_tag="JN749_001793"
     CDS             19593..19916
                     /locus_tag="JN749_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7368237.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            19913..20323
                     /locus_tag="JN749_001794"
     CDS             19913..20323
                     /locus_tag="JN749_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7368238.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRLRVDMPADNFGVEPQYL
                     ITFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECGQSHGGNNDDFLFAR"
     gene            20298..20804
                     /locus_tag="JN749_001795"
     CDS             20298..20804
                     /locus_tag="JN749_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368239.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            20801..21361
                     /locus_tag="JN749_001796"
     CDS             20801..21361
                     /locus_tag="JN749_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368240.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLRLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLEPGNGPDGDIEHHTEITLPS"
     gene            21370..21537
                     /locus_tag="JN749_001797"
     CDS             21370..21537
                     /locus_tag="JN749_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7368241.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDEL"
     gene            21524..23017
                     /locus_tag="JN749_001798"
     CDS             21524..23017
                     /locus_tag="JN749_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7368242.1"
                     /translation="MTSFNTIPSNTLVPIFYAEMDNSAANTAQDSGASLLIGHANNGA
                     EIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPEATGAAATVT
                     LTVTGEATESGTVNVYVGRTRVQAPVTNGDNVTTIASSIQDAINAVPALPFTASSSAG
                     VVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAAM
                     ADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAGD
                     QFNQQHITLAGYEKETQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLPAPKGK
                     RFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAYV
                     LRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYEL
                     FKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            23017..23373
                     /locus_tag="JN749_001799"
     CDS             23017..23373
                     /locus_tag="JN749_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7368243.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPASKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            23373..23642
                     /locus_tag="JN749_001800"
     CDS             23373..23642
                     /locus_tag="JN749_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HAY7368244.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            23784..25619
                     /locus_tag="JN749_001801"
     CDS             23784..25619
                     /locus_tag="JN749_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7368245.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETISPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYIPYPAVDLAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
BASE COUNT         6394 a         6012 c         7056 g         6240 t
ORIGIN      
        1 gggggtaatc cgtatttctc acggattacg gagtctattt tttcttccat ttgtttatag
       61 tcaggaagaa ggcgtttcag tggtgcggga atgtcctggc aatacgcttc tgttgcatca
      121 tgcattaacg cttcaaaagc aaattcctgc ggcaccagct ggctgcaaag aaccgcatgt
      181 tgggcgacgc tgtagaagtg cgaaagatga ccggcaaagc gacagatatt tgaaagggaa
      241 accgcgatat cgttaatatc gatgtcgtct ttatttatcc tgtcataata aaaatgcttc
      301 ccggaaaaag ttttaataaa tgacattttg ttctccacgt atatgcgctg caccgcgctg
      361 aattctggta aaaagaatcc ctcaccatcc ggcgattatt gagtaaatta cgtttccata
      421 aatgcccccg caggggcatt tgcagtaatg aaatcaggcg gtgaaagtac caataaaggt
      481 ttctactttg ctgtccttga atttctcaac aagcagatca cgaaattcgt tagccatttc
      541 ttcctgcacc gcctccagct gaataatgcg cagaaccagt accggacgat cgccagtgat
      601 aatgctgagg cgtaatttaa acggacgttc tttcagacct tcaaacggaa tgcatttaaa
      661 ttcaaatgcc actggcataa tgtctttggt cttcgcttcg acagactcca tcagggagcg
      721 tttgccgctg aagtcattat cttcaaaatc agcggtctgg tttgcttcaa tcgtgatttt
      781 acggaccgcc gcagccgctt ttgttgcctg aatggtgtca ccattagcat caaagcccac
      841 aaggtagtcg gcccagtctt caatccattc tgccagtgat ttctgggagt tacgctcgcc
      901 attaacagac aacagagcag agaacggtgc tgtctttttc agtttgagag tggcggtgtt
      961 atctgcgtga cctggttcat caatagtacc caggttaagc acactgacgg cacgcatatt
     1021 atcagcatcg ataaagcagc gggtgccttc atctgcaaga tctttagaat aacgggtaaa
     1081 gtcatcgatg ctggcagtgg aaagcgcacc acggaaacgg aagcgattta aattaaattt
     1141 ttccagatca tgaatgcgga aattctcagg caatgccaca gcatcggcac caatcttact
     1201 gataatttca ttaacaccct gagcagaaat aagggcatgg atttgattaa ttgcggttgc
     1261 gtctaagttc tgagacataa taagtcctaa ctatataaag atattcagtg atgagataaa
     1321 taatcagtta attaagaacg atattaatga cctgctgcgc ggagttttcc gtcaggttca
     1381 ccggcaagag tcagtaattg tccctggtct tcctgcagaa tagtcaggcg accaccgcga
     1441 ttgacataca tcggcgtttc ggtggtgtct tcttcggaaa ttttcccgcg gttagtcggg
     1501 cgaacatatg agagtttgtg tttgattttc acacggttct catcaaatgg ttcgatttcc
     1561 aggttgagtg agaccttacc tttggttttc gtgttcatca caccggaagc gacttcactg
     1621 agaactgcgc cgattttggt ttcaaatacg ccgccgtcca gctccccgat aaatgcctgc
     1681 acatcagtac tgcgttcgct agccattttg ctgctcctca tcatatcgac cctgcaaggc
     1741 cgattagttt ctccacaaaa cagagaagaa cacctgcggt agcagccgcc cggatggatt
     1801 gggttatgag cccgtcgtcc ggtgatgctc ttctctgttt tgtaaaaagg acggtaccag
     1861 ccggaagcaa gggtacaaac tggtaccgcc aggactacac acagcataaa gttgtggtgc
     1921 cgggtgcctc ccggtgcctg gcgaaggttg cacaccagac gggtgggtat ccacagaagg
     1981 tcgactgtca gcctcaacct taacccgcgt gcgctgagcc gcattcacca caactctaag
     2041 gattctcttt ggttgaaaat acttagctgt tatgtgcctg tcttttcacc acttcaggct
     2101 cggtggtatc cttttaagcc cgtatacata aaaggaaaat caaatgactt ttgatgaaaa
     2161 agaacttgat aatgcaatta ataaaatcat cgtaacgtcg ctcttttcct gtctcagcga
     2221 cactcagcag aaacagttct acgaatcggc tttcaacatg atcgagcgtt gttgtttctg
     2281 cgatgccgac gagttacctg aaaaaatcag gaaacagttg gctgatgctc ttcgagtgcg
     2341 actttctgac caattttctg aaatgtgctc tccgaatttg gacaaataga aaaaggccat
     2401 ttccattcag ggtctgatgg aaatacttca gcctgttcca aagcacggcg taaagagaac
     2461 acaactccag ccataatctg atgtttccca ttggtccagc tatcgccgct ctgatctaca
     2521 ggggcggcta tgtcgtatga ccaaacgact tcacagttat tgtttaaaat ctggactttc
     2581 atttcataca cctgctttaa catgagtgcc tagtggcaca acatgactca acgaatcatc
     2641 ctggacttca tatgccccag gcggctactt cgtgggcgtc ctgcctgttc gttgtttcgc
     2701 ttgggtacat tatgtatccc aagggtacat tgtcaagtat aaaaaaacct gccgaagcag
     2761 gttcataaac attgattagg ctttgatttt gtatcttctt ggttttcctg agaaaatcac
     2821 tgtaccaatt atagagcaat taccgttgat cttaatgtaa ggctcaggcc agtttgggtt
     2881 taacgctttg agataacgct gtgtcccatc ttctatcaac cttttgaagg tggtttcgcc
     2941 tgtatcgtgc atcaatgcaa taacgtcgtc accgtggcag gcaggtactt caggatcgac
     3001 aaaaatcatg tctcccgggc ggtactcatc aatcattgaa tcacctatca cccgcaagat
     3061 ataagtcatt tccccacagg gtacagggca gggatacgtt tctgctgtgc tcaaatcaac
     3121 ctcagaatat ccaacttctt tccatgctcc ggcctgtacc catgatatga cagggactaa
     3181 tgtgatttgt ttattagtga ttgaaacatc aggttttttt gtgatgttcg ttgtctggtg
     3241 ttcttgatcg agccatccga caggcaggtc gaaacatttt tcgatgtgtc gtgccatgct
     3301 gtcaccgata tttttagtag caccatctcc cataaacctg ctggtctggg ttggctcgcg
     3361 atcaatcata gtggcaaagg aagaattccc gccaacacca tctctcagtt ttctggcgtt
     3421 agaccgccgg atgtcatgga ttgttttcat aacgaaatta aaacccttgt accgttaagg
     3481 tacaagtgtc ttggaggttc atttcaatca tgtaacatgt acaccggagg tacatattgt
     3541 atgaaagcgt attgggactc tttaaccaaa gaacagcagg gcgagttggc cggaaaagtt
     3601 ggctcaacac ctggctactt acggctggtt ttcaatggct ataaaaaagc cagttttgtg
     3661 ctggctaaaa aacttgagca atgcacgtca ggtgcaatta cgaaatctga cttaagaccg
     3721 gatatctatc cgaaagatta gcagaacact ttcaattttt aaccacagaa cgatgaggct
     3781 aaccgtgggt aagcatcact ggaaaataga aaaacagcct gagtggtacg tgaaagctgt
     3841 cagaaaaact atcgcggcgt tgccgggtgg ttacgctgaa gcggctgact ggctcgatgt
     3901 aacagaaaac gctttattca accgccttcg tgcagatggc gatcagattt tcccgctggg
     3961 atgggcaatg gttttacagc gtgctggtgg cactcacttc attgctgatg ctgtggcgca
     4021 gtctgcaaat ggcgtctttg tgtctcttcc tgacgtcgag gatgtggaca acgccgatat
     4081 caaccaacgc ctgctggaag tcattgaaca gatcggcagt tattcaaaac agattcgttc
     4141 agcaattgaa gacggtgtag tggaaccgca tgagaagaca gcaattaacg atgagcagta
     4201 cctctcaatt tcgaagctgc aggagcatgc agcactggtc tacaaaatct tttgcgtttc
     4261 agaaagtagt gacgcccgcg agtgtgcagc tccgggcgcc gtggcatgtc gtgactgtgg
     4321 agaaactaac gcatgaacag tttaacaaca cactaccgtc gctcgcaact gattgcgctt
     4381 cctgtaccgg gtggaaaagc gaaggtggag tattgctatg cagtaaatgt accaggtgac
     4441 agggaaattg taacccacag ctttgcagag tgggctgtgg gtgatttcaa ccggcagaag
     4501 gagacagtcc tttgcgacaa gttaaccgct ggttcaaaga gcactacgga gtgcccgtca
     4561 gagtcattcg ttgggagccg gaaacacaac gtgttatcta cctccgcgaa ggctatgagc
     4621 atgagtgctt cagcccgctc gaacagtttc gtcgtaaatt cagggaaata gaggtcggtc
     4681 atgagcacta aattaaccgg ctatgtatgg gatggttgcg ctgcatcagg catgaaatta
     4741 tccagcgtgg caattatggc ccgcctggct gatttcagta atgacgaagg tgtgtgctgg
     4801 ccatcaattg aaaccattgc ccgccagatt ggcgcgggaa tgagtaccgt cagaacggct
     4861 atcgcacggc tggaagcaga aggctggtta acgcgtaagg cgcgtcgcca gggtaaccgc
     4921 aatgcgtcga atgtttatca gcttaacgtt gcgaagcttc aggcagcggc attttctcaa
     4981 ctgtcagatt ctgacccgtc aaaatctgac gcatcaaaat ctgacccgtc aaaatttgat
     5041 gcgtcgaaat ctggcaaaaa agcgggtttt cacccgtcag aatctggcgg ggatccgtca
     5101 gtaaaatcaa aacatgatcc gtcagataaa aaaacttctc gtccggacgc ttcgcaaccg
     5161 gacacgcaga cggctgaaca ggagttttta actcgccatc ctgatgcggt tgtattcagc
     5221 cctaaaaagc gccagtgggg aacgcaggat gatttgacct gcgcacagtg gctctggaaa
     5281 aaaatcatcg ccctgtacga gcaggccgcc gaatgtgacg gcgaggtggt tcgtcccaaa
     5341 gaaccgaact ggacagcctg ggcaaacgaa attcgcctga tgtgtgtgca ggatggtcgt
     5401 actcataaac aaatctgcga gatgtacagc cgcgtcagtc gcgatccgtt ctggtgccgt
     5461 aacgtgctca gcccgtcgaa gctgcgggaa aaatgggatg agctttccct gcgcttatcg
     5521 ccgtccgtca gcacgtacac cgaaaaacgc gaagacccgt acttcaaagc tagttacgac
     5581 aacgtggact acagccaaat cccggcagga ttcagggggt gagcatgagt cttttgaatg
     5641 acgttcagaa attcattgaa gcccatcggg gctgtacttc cggagatatt gcggatgctt
     5701 ttgccggtta ctcacggcag cgcgttctgc agtcagcaag caagttacgt cagagtgggc
     5761 gtgtggctca ccgttgtgaa ggagatacac gcagacattt cccgcgcctg actgagagag
     5821 cgcaggaacc ggaaccacaa ccagttcgtg aaaccagacc tgtgcgcaat ttctatgtcg
     5881 gcactaacga tccccgtgtg attttgtgcc tgacccgcca ggcggaagaa ctggagtcca
     5941 ggggcttata ccgtcgtgct gcaaccgtgt ggatggcggc attccgtgaa agccactccc
     6001 agccagaacg aaacaatttt ctggcgcgtc gtgagcggtg cttacggaaa agcagcaagc
     6061 gcgctgcatc gggtgaagag tggtatctgt cagggaatta cgtgggggct taatgagtaa
     6121 taaatattgc caggcgctgg tggaactgcg gaacaaacca gcccatgaac tgaaggaagt
     6181 gggcgatcag tggcgcacgc cggacaacat tttctgggga attaacaccc tgtttggccc
     6241 gtttgttctg gatctgttca ctgacggtga taacgccaaa tgtgccgcgt attacacggc
     6301 ggaagacaac gcgctggcgc atgactggtc agaacgtctt gcggagctta aaggtgctgc
     6361 ctttggtaat cccccataca gccgcgccag tcagcatgag gggcaataca tcaccggcat
     6421 gcgttacatc atgaaacatg ccagtgccat gcgtgataag ggcgggcgct atgttttcct
     6481 gatcaaagct gccaccagcg aagtgtggtg gccggaagat gcagatcata ttgcttttat
     6541 tcgcgggcgt attggttttg aactgcctgt ctggtttatc ccgaaagacg agaagcaggt
     6601 actgacagga gcttttttcg ctggtgctat tgctgttttt gacaagacct ggaagggacc
     6661 ggcaatcagc tacatcgggc gcgatgaact tgaggcatgt ggtgaggcgt ttctggcgca
     6721 ggttcgccag caggcggaaa aactggtcag ggagatggcg gcatgacgac gttaactcaa
     6781 tgccagcagc aggtgctgga tatgctgatt tcttatcaga aagaacgtgg cttcccgcca
     6841 accaatcagg aggtggcaac catgctggga taccgttcgg tgaatgcagc ggtggagcat
     6901 cttcgcgcac tggagaaaaa aggcgtcatc acgataaagc gtggcgtggc ccgggggata
     6961 acgcttcata ccgcggtgaa ggacgacgac agcgaggcgg tcgggattat ccgcgcactg
     7021 cttgccggtg aggaaaacgc aaggctgcgt gcaacccact ggttacatga gagggacctg
     7081 aaagtatgaa gctgatcctg ccttttccgc ccagcgtgaa cacgtactgg cgacacccca
     7141 acaaaggggc gtttgctggt aagagcctga taagcgcggc ggggcgaaaa ttccagagcg
     7201 cggcgtgcgc agcaatagtt gagcagttac gtcgtctgcc gaaaccaacg tcggcacctg
     7261 cttcagtgga gatcgtgttg tttcctccgg ataaccggat ccgcgatctg gacaactata
     7321 acaaggcgct gtttgacgcc ctgacccacg cgggtgtgtg ggaagacgac agtcaggtga
     7381 aaagaatgct ggtggagtgg gggccggtta tcccggaagg gaaggtcgag atcactatca
     7441 ataagtacga gaaaacggcg ggtgcagccg cctgattaag aggagaaacg aagtatgaat
     7501 aatctgatgg tcattgatgg tattgaagtt cgtcgtgatg cttatgggcg ttacagcctg
     7561 aacgatctgc acagggctgc cggttctcag gataagcata agcctgcatt ctggctccgc
     7621 aatgagcaaa ccgaacgttt aataagcgag ttgcagattt gcaactcggt caatatagag
     7681 ccagttaacg ttagtcgtgg cggaaataac caggggacgt atgtctgcaa agaactggtg
     7741 tatgcctatg caatgtggat cagcccgtca ttccatctga aggtgatccg tactttcgat
     7801 atggtaacca gcgcaccgga aaaattatcc gggcaggctg ctgacaagat gcaggctggc
     7861 gtgattctgc tggactttat gcggcgggag ttaaacctgt ctaactcatc tgtgcttggg
     7921 gcctgtcaga aactccagga ggctgttggc ttaccgaatc tggcaccacg ctatgccatt
     7981 gatgctcctg ctgacgcgcc tgatggctca agtcgcccta cgctgtcgct gagtgcactg
     8041 ctgaaacagt atggtatccg cctgacagct aatcaggcat atcaccagat ggcgaagctg
     8101 gggatcgttg aacaacgcga acgatacagc cgtaccgcga ttaacaacat caaaaaattc
     8161 tggtcgctga cggcgaaagg ctgcatgttc ggcaagaaca ttaccagtcc tgcaaatccg
     8221 cgcgagacgc agccgcattt cttcgaatcc cgattccctg agctgttaaa gctgctcgat
     8281 accgttcatt gaggtgaccg tgagagcact actgacccct gaaattgccc cgcgtatggg
     8341 gatcgtattg ttcagaccag gttcagagct gatgcccctg tttatgcagg ggcgtgtact
     8401 gctggagcct gagccggaac gttattcatc tttcgccagt ggtgccgttc ccgcggcatc
     8461 acaaccgctg gcggatgatc ctgctgttcg ggccgtgttc cgcaatgagg cagtgatccg
     8521 tcgtgctggt ggcgtggaat gtcttgaaag ctggctactt cgtgaaaaag gctgccagtg
     8581 gcctcattcc gactggcaca gcgagaacat gaccacaatg cgacacgctc cgggcgcaat
     8641 ccgtctgtgc tggcactgtg ataaccagct gcgcgatcag ttcacggaac ggctggaatc
     8701 aatggcaacg gataactgtg cccgctgggt gttgtctgtt gtgcgtcggg atctcggttt
     8761 tgatgatagt cacgttgtga caatgccgga actgtgctgg tggctgattc gtaatgacct
     8821 ggcggatgcc ttaccggaaa gtgcagcccg taaggcactg agattaccaa agcctgttgt
     8881 gccgtctgtc acccgggaaa gtgaccttgt gccttcggtt cctgccacca gcatcatcca
     8941 agataaggca aaaaaggtgc tggcgctgaa agtggatccg gagtcgccgg agtcttttat
     9001 gttacgccca aaacgtcgcc gctgggttaa tgagaagtac acgcgctggg ttaagacaca
     9061 gccgtgtgca tgttgtggaa agcccgctga tgatccccac cacctgatag gccacggtca
     9121 ggggggaatg ggtacaaaag cgcatgacct ctttgtgttg cctttgtgca gaaagcatca
     9181 cgacgagctg catgcggata ccgtggcatt tgaagagaag tatggctccc agctggagct
     9241 gatatttcgt tttatcgatc gtgcgctggc aattggcgtg ctggcctgat ttggtggaga
     9301 aagttgatgc gtgatattca gatggttctt gagcgttggg gagcatgggc ggcgagtgat
     9361 agttcaggcg ttgactattc acctatagct gctgggttta agggacttct tccctatacc
     9421 agcaagaaac gcttggcttg ttcggatagt gatgcattaa ttattgaagg ttgtcttgct
     9481 cgtctaaagc aaaaaagacc ttatgagcat tcgcttcttg tggcacatta tttatacaga
     9541 atatccaagc gtaagattgc aaaggcgcgt ggaaaggatg agaaattgat acgtatagaa
     9601 atacagctag ccgaaggatt tattgatggc tgcctttcca tgctagatct aacattagat
     9661 ttggacgttt aataatacgc cccatgcagg ggcgtattat ttactggatg aatgacattt
     9721 gattaatata ttttatcaat aactctctgg gtgtagtact ccaaaaattt aggtgttcat
     9781 aatcagtata aacagtggtg aattttttaa gttcttcatc acttttaggg gcaaatcgct
     9841 cattaagagt aatactgtct ttaattattc cgaagaatac aatagatatt tcaggaattt
     9901 gcgatcttct ctcataagaa taaggtattt cttttatgtt aagcagatct gctaaatgag
     9961 atatagattc tgcggtaatt tcttgtttta gtttttcctt gtcaccaaag caatgtatga
    10021 agacagctac gacaaagatc ttatttatta aaggatcact gcttttgtac tcatcgtcgt
    10081 ttaatagttg aaaaatgtta agattgcgtg aaaaagtttg tgtttcacgt aaggataagt
    10141 tggttcgttg aattaaatca cggataaatc taccagataa cctattaatt tcatttaatg
    10201 attttgtctt gtctacaagg taattccaat atataacaga tgttttacat acgacgtgac
    10261 catttattaa acatgtgtct ggaagcgtga tggtatattt tataaacttg tcaagatact
    10321 tttgtgagtt aatactataa ccataaatat gatttatcga tgcttttaat tgttctgtgt
    10381 ttgtaactaa aataaaaaag acattattga tgtcaaaaat gtgttttatt gtttcaatga
    10441 catttgttga aaaactcggc ttacatcggt ctaattcatc aattataatt actatctttt
    10501 gactttctga aatgctttcg atgcaagatt taagtgaatt tatgtttttc tctgattcca
    10561 tatggtcttc aagcaaattt tcaatagtcc catctattgc tgcattgctt gctttcttca
    10621 tcgcatcttg gaattcttcg gaaacttcac tagcctcctg tcgtaaaaac caacctgcac
    10681 cagcttttag taccgttttt aaaccaaagc gaattgcagg aagagatctc ttaatgaagt
    10741 gttgtttttc ttcctcaggt aaaatgctgg caattgcaga ggttatgaga agtaatggag
    10801 attctgcatg atccccttta aaggcatcaa tataaacaac tttagattca gtttcttgct
    10861 caataatgag atttttcagt ttgatactaa attctgtttt ccctgttccc catgcgccgt
    10921 ctattaccag tggtgaaatg tctgcctctg gttttagcaa cttgatgata ttttcagcga
    10981 tgtttctgcg ttggaactcg tcacgttcag tgaaagatag tgtatctaac ataatataaa
    11041 cctattaact cctacagtaa aaaacgaata atacgacttt gggattaaaa atcattaacg
    11101 cggtccgcaa aaattcttgt aatctgttaa gagtggttac ttcgccacac agcttaaacc
    11161 cgccgtcgag cgggttttgt cgtttttgtt gctggggatt cgttgggcct ggcctatccc
    11221 gcagttatcc attggttcgg ctctttgacg tttccgcttc tgatttgcgg tacatgatgt
    11281 tccctcaatt tgcacctgct gtatcagcga ggtgagagat aactacaaat gcctcataac
    11341 ccaaatacct ggttggagtt ggtccagagc tggtggcgtg gagacacacc gctgggcgca
    11401 gtgattatgt cgattgttat ggctggcttg cgcattgcat attttggcgg tggtgggggc
    11461 tggaaacgaa aaacgctcga gattttgctc tgtggtgctc tgacgctgac ttttgcatcc
    11521 gctcttgagt atgtcggatg gcctaaatct ctttctgttg ccattggtgg cggcgttggg
    11581 ctaatcgggg tcgatgctat tcgtggggct gcaatgagag taatcggtaa taagtttggt
    11641 ggctctaagg agtaatttat gcaggtacta aattcccagc gtaaagcttt cctcgatatg
    11701 gtggcatggt cagaaggaac ggataacggg cgacaaccga cacgtaatca cggttatgat
    11761 gttattgttg gcggcgaact gtttactgat tactctgatc accctcgcaa acttgtcacg
    11821 ctaaacccca aactcaaatc aacagccgcc ggacgttatc agcttctttc acgctggtgg
    11881 gatgcctacc gtaagcagct tgctctgaaa gacttctccc ccaaaagcca ggatgctgtg
    11941 gcactgcaac agattaaaga gcgtggcgct ttaccgatga ttgatcgcgg tgatattcgt
    12001 caggctatcg accgttgcag caatatctgg gcgtcgttac ccggtgcagg ttatggtcag
    12061 tatgaacata aaatcggtga tttgattgcc cggtttaaag aggctggtgg ggtggtaaat
    12121 gaagctgagc tataagctgc ttatcgttgg tttcttcgtt actgtcattg gttctttcat
    12181 ctggtcggcc aaccactact acagcaaata tcagtacgaa aagaaacgtg ctgatgaggc
    12241 tgtacgaaat gctgaatcag caactgccat taccaataac gtcctgcaat cactacaaat
    12301 cgtcaataca gttctggagg ctaaccagca tgcaaaacag cagatcgcac tggagtcaca
    12361 gagagcccgg gaagatatca aagtggctgt tgcggatgat gattgtgctt cacgccctgt
    12421 gcctgatgtc gctgctgacc ggttgcggaa gtacgcggac agtatacgtg caggttccaa
    12481 cgatgccgtt accgacgaac ctgctcgctg aaactccaca gccagttata cccaatcctc
    12541 tgacttatgg ggatagtctt agtttgaatg taagtctgct atcagcactg gggctatgta
    12601 accgtgataa gtctgatctt cgtaggttag gagagcaaaa gtacaatctg catttgaata
    12661 ataatattca ttaggtgaaa tattattatt tgactgttct agttattatg catttagtta
    12721 caatactctc actattaaca gtgaggtaaa aatgaacgaa aattatattg catatgagac
    12781 acttgtagca aaccgtgctg ctgctgagtg ggcttgttgg gcaatgattg catcttgggt
    12841 gagtgctgga gctactattg ttactttggt tttggcgttc aaggcattgt ttacgtggcg
    12901 ggaacaagag aaaacaaaag taaaaataga ttttaggaat gcattaaaga aattaaagac
    12961 agctctatta tttatgcctg tcaatattga ccccgagaaa ctcaatgatg aacgagagca
    13021 agttattgcg aaatggctat ttaaagatgt agatcttatt agtcagcaaa ttgagttggg
    13081 agaagagaat gttaaaagat ttgatgaact tttgagtatt ttcgattgtt gccagtcttc
    13141 atggtttgcg acagagcact tatttgataa tactgagtta gaaaaagttt ggcatgagtt
    13201 cgagtctaac tttaataaat atataaatgg tggtgagagt aaggatttac ttatgaaaat
    13261 gcttgataag ctcatctctt ctagatttgt atttgagtca agataattgc ctttgagtat
    13321 ttttctttat tattttactt attataaatt ttttatatgc cccctagaat cccaaaagcc
    13381 tgccgtgttc gcggctgccg ctctacaacc acggacccgt caggctactg cgaaagccat
    13441 aaaagcgaag gctggaagca atacaagcca ggacaatctc gtcatcagcg cggttatggt
    13501 tcgaagtggg atgttatccg cgtgcgtgtc ctgaagcgtg acaaaggcct gtgtcagcta
    13561 tgtctgcgtg ctggtgtggt gcgtgaggcg aaaactgttg accacatcat ccctaaagcg
    13621 catggcggca cagatgccga cagcaatctg cagagcctgt gctggccgtg ccataaggag
    13681 aagacggccc gtgaacggct aaagtgatca taattctcaa ctgtctgagg ggaggggcgg
    13741 gtcaaatctc tgtgacctga cgtcttccgg actgcccgcc ccatcgtttt tttatacccg
    13801 cgaaaaatga aatttaacca ggagtgccgc atatggctgg aacggcgggg cgttccgggc
    13861 gtcgccctaa gccaacggcg cgcaaggcgc tggccggaaa ccccggcaag cgagccctga
    13921 acaaagatga acctgttttt acgcccatca aaggtgttga gccaccggag tggttcgctg
    13981 aagaagagct tcctctcgcc acgatcatgt ggcaactgac aaccaaagaa ctctgcggtc
    14041 agggcctgct gtgcgtgact gaccttgcgg tgcttgagcg gtggtgtgtg gcctatgagt
    14101 tctggcggcg tgccgtgaaa aatattgcca gacagggcaa caccatcacc ggtgcaatgg
    14161 gcggtatggt caaaaatccg gagctgaccg ccaaaaaaga acaggagtcc gagatgagca
    14221 gcacgggggc aatgctcgga ctcgacccca gtagccgcca gcgtctgatt ggcctggcgg
    14281 ggcagaagaa agccactaac ccgtttctga aaatcatcga gtcatgagcc ggaaatctta
    14341 ccccaacgta aatgctgcca atcagtatgc ccgtgatgtc gtgcgcggaa agattgtggc
    14401 ctgccagttt gtgattcagg cctgccagcg ccatcttgat gacctgatgg cggaaaaaag
    14461 taagtcgttt cgttaccgct tcgacaagga gctggctgaa cgggccgcga aatttattca
    14521 gctgttgcca cacaccaagg gggagtgggc attcaagagg atgcccatca cgctggagcc
    14581 gtggcagctc tttgtgatct gctgtgcgtt tggctgggtc aataaagggt cccggctgcg
    14641 ccgcttccgg gaggtgtata ccgaaatccc ccgtaagaac ggcaaatcgg caatctctgc
    14701 cggtgttgcc ctgtattgtt ttgcctgtga taacgagttt ggcgcggaag tgtattccgg
    14761 tgccacgaca gagaaacaga cgtgggaagt ctttcgcccg gcgcgactga tgtgtaaacg
    14821 cacacccatg ctgacggaag cgttcgggat tgaggttaac gcctcaaaca tgaaccgtcc
    14881 ggaggatggg gcgcggtttg aaccgctgat tggtaacccc ggtgatggtt catcacccca
    14941 ctgtgcggtg gtggatgaat atcacgagca cgccacagat gcgctttaca ccacgatgct
    15001 taccgggatg ggggcgcgac gtcagccact gatgtgggct atcactaccg ccgggtacaa
    15061 cattgagggg ccgtgctacg acaaacggcg ggaagtcatc gagatgctca acggttcggt
    15121 acccaacgat gaactgttcg ggattatcta taccgttgat gaaggtgacg actggaccga
    15181 cccgcaggtg ctggaaaaag ccaatccaaa tattggcgtg tcggtttatc gcgaattttt
    15241 gttaagtcag cagcagcgtg cgaaaaataa cgcccgtctg gcaaacgtct ttaaaacaaa
    15301 acacctcaat atctgggtgt cggcgcgttc ggcgtatttc aacctggtga gctggcagag
    15361 ctgcgaggat aaatcactga cccttgagca gttcgagggg cagccgtgca ttctggcctt
    15421 tgacctggcg cgtaagctgg atatgaacag catggcgcga ctttataccc gcgagattga
    15481 cggtaaaacg cattactaca gtgtggcccc gcgtttctgg gtaccgtatg acacggtgta
    15541 cagcgtcgag aaaaatgaag atcgacggac agccgaacgc tttcagaaat gggtggaaat
    15601 gggcgttctg accgttaccg atggtgcgga ggtggattat cgctacatcc tcgaggaggc
    15661 caaagcggcg aacaaaatca gtccggtcag tgagtcaccc atcgacccct tcggggcgac
    15721 cgggttgtca catgaccttg ctgatgaaga cctgaacccc atcactatca ttcagaacta
    15781 caccaacatg tccgacccga tgaaagagct ggaagcggca attgaatcgg ggcgctttca
    15841 tcatgatggc aatcccatca tgacctagtg tatcggcaac gtggtcggca aaaccattcc
    15901 gggtaacgat gatgtggtga agcccgtcaa agagcaggcg gaaaacaaaa ttgacggtgc
    15961 agttgcgctg attatggcgg ttggcagagc catgctgtac gagaaagaag acacgctgtc
    16021 tgaccacatt gagtcctatg ggatccgctc gctttaactg aggtaattat gatcatgctg
    16081 attctcgcgc ctctggtggg cgtgctgggg gcgcttttgc tggcgtatgg tgcctggctg
    16141 atttatcccc cggcggggtt tgttgttgcc ggggcgttgt gcctgttctg gtcgtggctg
    16201 gtggcgcgat atctcgaccg tacacagtcg tctgtcggcg gaggtaaata gtgttctttt
    16261 cgggattatt tcaacgaaaa agtgacgcac cggtgaccac gccagcagag ctggcggatg
    16321 atatcgggtt gtcctacgac acctataccg gaaagcagat cagcagccag cgggccatgc
    16381 gactgacggc ggttttttcc tgtgtcaggg tgctggcgga gtcggtcggg atgttgccct
    16441 gcaacctgta tcacctgaac ggcagcctga agcagagagc cactggcgaa cgtctgcata
    16501 agctgatctc cacgcatccc aatggctata tgacgccgca ggagttctgg gagctggtgg
    16561 tcacctgtct gtgcctgcgg ggaaactttt acgcctacaa agtgaaagca tttggcgaag
    16621 tggctgaact gctgcccgtc gatcccagct gtgtggtacc gaagcttaac agtagctggg
    16681 agccggtcta tcaggtcaca ttcccggatg gctccgcgga tgtactgagc caggaggata
    16741 tctggcatgt gcgcacgctg acgctggacg gactggtggg gctgaatccc atcgcctatg
    16801 cccgcgaggc aatatcgctg gcggcagcga ccgaagagca cggggccaga ctgttcagca
    16861 atggcgcggt gacgtcgggt gtgttgcgta cagagcagac gctgtcagat caggcttacg
    16921 agcgcctgaa gaaagatttt gaggagcgtc acaccgggct tggcaatgct caccgcccga
    16981 tgatccttga gatggggctg gactggaagt cgatggcgct gaacgccgag gacagccagt
    17041 tcctggaaac ccgcaagttt cagcttgaag aaatctgtcg tctgttccgg gtgccgttgc
    17101 acatggtgca gaacaccgat cgcgccactt tcaacaatat tgaagagctg gggctgggat
    17161 ttatcaacta ttcactggtg ccgtatctga cccgcatcga acagcggatc aacaccggac
    17221 tggtacgaaa aagtaagcag ggcgtttatt acgccaaatt taacgccggg gcgttactgc
    17281 gcggggatat gaagtcccgt tttgaagcct acgccaccgg gattaactgg ggaatttact
    17341 ctcccaatga ctgccgcgac ctggaagata tgaatccgcg tcccggtggt gatgtctatc
    17401 tcacaccgat gaacatgacc acgaaaccct ccgatggcag taaagccggt aagcagaagg
    17461 ataacgccaa tgcagacgaa acaacgtctt gatgtaccgc tgagtctgaa atctgtcagt
    17521 gactccggtg agtttgaagg gtatggctcc gtctttggtg taaaggacag ccacgatgat
    17581 gtggtgatgt ccggggcatt tgccgcttcc ctgcgggcgt ggagtgacag aaaagcgtta
    17641 cctgcgctgc tctggcagca ccgcatggat gagcccatcg gtgtttacac cgaaatgaag
    17701 gaagacgatg tcgggcttta cgtcaggggg cggttgctca ttgatgatga tcccctggca
    17761 aaacgcgcac atgcacacat gaaggccggt tcgttaaccg gcctttctat tgggtacgtc
    17821 ctgaaggact gggaatacga ccggacgaaa gaagcctttc tgctgaaaga aatcgacctc
    17881 tgggaagtca gtctggtgac gtttccgtct aacgacgagg cgcggatcag cgacgtcaag
    17941 aacgcgctgg cccgcgggga aatccccgaa cagaaaaaaa tcgaaagagt cctgcgtgat
    18001 gtcggactct cccgtaccca agccaaagca ttcatggccg ggggctatat cgcactgtcc
    18061 ctgcgcgacg ctgaggatgt gggctctgca ctgaatgcac tgaaaaatct gaacttctaa
    18121 tcaggagaaa tacgatggcg gtagatatta aagatgtcga acaggtcgcg caggagctgc
    18181 agcagaagtt tgacgacttc aaagcaaaga acgacaagcg cgtggatgcg attgagcagg
    18241 aaaaaggcaa gcttgccggg caggtggaaa ccctgaacgg gaaactcagc gagctggaaa
    18301 atctcaaaag cgatcttgaa aaagagctgc ttgagctgaa acgtccggca ggtggcgcgc
    18361 aaaataaact ggccaccgag cataaagaag cgtttgtggg ctttttgcgt aaaggccgtg
    18421 aagatggtct gcgcgatctg gagcgtaagg cattgcaggt gggcaccgat gaagacggtg
    18481 gctatgccgt gccggaagcg ctggatcgca acattctcac cctgctgaaa gatgaagtgg
    18541 tgatgcgcca ggaagccacg gtgatcaccg ttggcggttc cgactacaaa aaactggtga
    18601 atctgggcgg cacggcttcc ggatgggtgg gggaaacgga tacgcgatcc cagactgcca
    18661 cctccagact ggagctgatt gaacctctca tgggggaaat ctacggcaac ccgcaggcca
    18721 cccagaaaat gctggacgat gccttcttca acgtggaggc ctggatcaac agcgagctgg
    18781 caaccgaatt tgccgaacag gaagaaattg cctttaccac cggcgatggt accaagaagc
    18841 cgaaagggtt cctggcgtat gaatccactg atgaaaccga taaggtccgg gcgttcggca
    18901 aacttcagca tattgtatcc ggcgaagcga cggcggtgac cgcagacgcc attatcaaac
    18961 tgatttacac gctgcgtaag gcacaccgca ccggcgcgaa gttcatgatg aacaacaaca
    19021 gcctgtttgc catccgtctg cttaaagaca gcgagggtaa ctatctgtgg cgtccggggc
    19081 tggaactggg gcagccgtcc tctctggcgg gttacggtat cgctgaaaac gaacagatgc
    19141 cggatatcgc cgctgatgcg aaagccattg catttggtaa cttcaaacgg ggttacacca
    19201 tcgttgaccg tatcggcacc cgcattctgc gtgacccgta caccaataaa ccgtttgtcg
    19261 gtttttatac caccaagcgc accggcggga tgctggtcga ttcgcaggcc atcaaactgc
    19321 tgaagattgc agcggcgtaa tcactcaggg gcgcggaacc gcgccccctg ttctgacggg
    19381 tgaagaatca tgatcctgaa acaagatctg aaatggtcac cggacggtat gcgtgttgag
    19441 gtcattcggg ccggtgagta tgacgacggg gcgcttcctg cccgggtgca ggagattgca
    19501 cttcaggccg ggttagcaga gcgcggaacc agtgcaaaaa gcagtaaagc gacaaaagag
    19561 aaaaaagcca cgaccagtaa agagggctga gtatgcttct gacaatggaa gagattaaag
    19621 cccaactccg gctggatgag gatttcgatg ctgatgaccg ccatctgcaa ctgctggcct
    19681 gtgcggcaca aaagcggacg gaaacgtatc tgaaccggaa gctctatgca ccggatgaaa
    19741 ccattccgga cagcgatccg gacgggctgc acctgccgga tgatattcgt ctggggatgc
    19801 tgatgcttat cagccatttt tacgaaaacc gctcgtcggt tacggaagtg gagaaacttg
    19861 acatgccgca gagttttggc tggcttgtcg gcccgtacag gtactttccg caatgaaaat
    19921 tcgtcaggcg cagaccagcg caacctacat tctgccggac cccggtgaac tgaataaacg
    19981 cgtcctgatc cgcctgcggg tggatatgcc cgcggataac tttggcgtgg agcctcaata
    20041 cctgattacg ttccggacat gggcgaaggt tatccagacc agtgccacca cctggcagga
    20101 aaccgcgcag accggggacg ccatcaccca ttacatcacc attcgttacc gccgggggat
    20161 taccgctgat tatgaggtgg tctgcggtga cagtgtgtac cgggtgaaac gtcagcgcga
    20221 tctgaacggg gcgcggcgct ttctgctgct ggagtgtacg gaactgggcg aatgtgggca
    20281 gagtcacgga ggcaacaatg acgacttcct ttttgcacgt tgattttcag cagcccgcgg
    20341 agatgcgctt taaccgcgcc cgtgtccggc gggcgtttgt cacgattggt cagcgtcata
    20401 tgcgtgatgc ccgtcggctg gtgatgcgcc gtgcgcggtc ggcaccgggt gaaaaccccg
    20461 gttatcagac cggacgcctg gctcgttcga ttggttacat ggtacccaga gccagtaaac
    20521 atcgccctgg ttttatggca cgtatagccc ctaaccagcg taatggagag ggaaaccgcc
    20581 gtatcaccgg tgatttttat ccggcttttt tgttctatgg cgtgaggcga ggggcaaagc
    20641 gtcgtcgcag ccatcatcgt ggtgcatccg gtggcagcgg ctggcgactg gctccacgta
    20701 ataactttat ggtggaaacg cttgaaaaga accgcagctg gacacgctat tttctggcgc
    20761 gggaattgcg taaatcactg aagccggagc gacgacacag atgaaactga cgcctgttat
    20821 tgctgcgctg cgtgcccgct gcccgtattt tgaaaaccgg gtggcaggcg cggcacagtt
    20881 caaaaatctg ccggaggtcg gaaagctgag actcccggcg gcgtatgtgg taccgggtga
    20941 tgactctccg ggagaaaaca aaagccagac cgactactgg caggagctga aagagggctt
    21001 ctccgtggtt gtcatactga gtaacgggcg tgatgagcgc ggtcagtttg cctcgtatga
    21061 tgtggtggac gatgtccggc aaatgctctt taaggccctg ctgggctgga acccggaagc
    21121 gtgcggtaac ccgattacct atgacggcgg cacgctgctg gatctgaatc gtcatgagct
    21181 gatttatcag ttcgattttt cggtcatcag cgagttgacc gaagacgata cccgccagca
    21241 ggatgacctg aacagtctgg atgaactgcg aacgctggcg attgatgttg attatctaga
    21301 gcccggtaac gggcctgacg gcgatatcga acatcacacc gaaataaccc ttccttcctg
    21361 agaatcttca tgtttgtgaa acctgttaaa gggcggtcag ttcctgaccc tgcccgcgga
    21421 gaccttttgc ccgccgaggg gcgaaatgtt gatgagaaca actactggct gcgccgtgaa
    21481 gcagcgggtg atatccggcg cgtgaatgaa aaggtgaata ccgatgacga gctttaacac
    21541 cattccgtca aatacgctgg ttccgatttt ttatgcggaa atggataact cggcggcgaa
    21601 tactgcacag gacagcggag catcgctgct gattggtcat gccaataacg gtgcagagat
    21661 tgttgccaac agtctggtgc tgatgccatc ggcagactat gcacgccaga tttgtggtgc
    21721 gggaagtcag ctggcgcgta tggtcgaggc ttatcgccag accgacccgt ttggtgagct
    21781 gtatgtgatt gccgttccag aagccacagg cgcggcggca acggttacgc tgacggtgac
    21841 cggggaagca accgaaagcg gcacggtgaa tgtctatgtg ggacgtaccc gcgtgcaggc
    21901 tccggtgacc aacggcgata acgtcacgac gattgccagc agtatccagg atgccatcaa
    21961 tgccgttccg gccctgccgt ttacggcttc atcttcggca ggcgtggtca cactgaccgc
    22021 gcgtcataag gggctttgcg ggaatgaaat tcctgtcagc ctcaattact acggctttgg
    22081 tgggggcgaa gtgctgccag cgggcgtaca gattgccgtg gcgacgggta ccgccggaac
    22141 gggtgctccg gttctcaccg gcgcggtggc tgcaatggcg gatgagccgt ttgattatat
    22201 cggcctgccg ttcaacgaca cggcctccgt taacacgctg gtgaccgaga tgaacgatac
    22261 cagcggtcgc tggagctatg cgcgtcagct gtatggtcat gtgtatacgg caaagatcgg
    22321 cacgctgtca gaactggtga ccgcaggtga ccagtttaac cagcagcaca ttaccctggc
    22381 gggatacgaa aaagagaccc agacgcctgc cgacgagctg gcggcaagcc gtaccgcccg
    22441 cgcagcggtg tttattcgca acgatccggc acgtcccacg cagaccggtg agctggtggg
    22501 tatgctgcct gcgccgaagg ggaaacggtt cacgatgacc gaacaacaga ccctgctgtc
    22561 tcatggcgtg gcaacggcgt atgtcgaaag cggggtgctg cgcattcagc gtgatgtcac
    22621 cacgtacagg aaaaacgctt acggtgttgc ggataacagc tacctcgaca gcgagacgct
    22681 gcataccagc gcgtatgtgc tgcgcaaact gaaatccgtc attaccagta agtacgggcg
    22741 tcacaagctt gccagcgacg gtacccgctt tggtcccggt caggcgattg tcacaccggc
    22801 ggtgatcaaa ggggaactgc tggcaaccta ccgtcagctt gagcgtgcgg ggatcgtgga
    22861 aaactacgaa ctgtttaagc agtacctggt tgtggagcgt gatgccagcg atccgaaccg
    22921 cctgaacacg ctgttcccgc ctgactatgt taaccagttg cgtgtctttg ccgtggttaa
    22981 ccagttccgt cttcagtatt cagaggagtc tgcataatgg cccgtatcgg gggaacctgt
    23041 tatttcaaaa ttgacggtca acagctatcg ctgaccggcg gcattgaggt gcccatgaac
    23101 aggacggtca atgatgacat catcggcctg gacggttcag tggaccgcaa ggaaactcac
    23161 cgtgcgcctt atgtcaaagg gaccttcaag gtgccgaaga attttccggc gagcaaaatc
    23221 acctcgtctg atgagatgac catcactgcc gagctggcga acggtcaggt ctatgtactg
    23281 tcgtctgcct ggctgcacgg cgaagcgaac cataatgccg aagaaggcac ggtcgatctt
    23341 gagttccacg gtgaagaagg ggattaccag taatgaaaga gcttgagtta aagaaaccga
    23401 ttaccgctca tggcgagaca ctctccgtac tggagtttga tgagcccacc gggaaagatg
    23461 tccgcgagct ggggtatccc taccagatga atcaggatga gtccgtcaga cttctggcgc
    23521 atgtggtatc gaaatatatt gtgcggctgg cgaaagtgcc gcaaagctct gtcgaccaga
    23581 tgtctccggc agacctgaat gcagcggcgt ggcttgtggc tggttttttc ctccaggcct
    23641 gacggctgaa taccttactg atcgcttctt tgattgcgcc agctactggc gcattaatcc
    23701 cttcgaattg ctgaatatgc cgatcagtga aattcccttg ctggtcagtc aggcaaacag
    23761 gatagagcag gagaaacgca cacatggctg aatttgagct taaggcgttg atcaccggtg
    23821 tcgacaggct ttctcccgcg ctgtcgaaaa tgcaaaagaa aatccgggga tttaaacgcc
    23881 aggcggaaga agcgtcacag ggtgggctgg cgcttggtgg cggactggca gcgggtctga
    23941 cgctttccct gaaatcttat gccgatcagg aaaacgccgc caccgggctg aaagtcgcca
    24001 tgatggatgc gaatggcgag gttggaaaga gctttcagga catcaataaa ctggctattg
    24061 gcctgggtaa ccagctaccc ggtacaacgg ctgatttcca gaacatgatg cagatgctgg
    24121 tgcgtcaggg gatcccggca gaaaatattc ttggcggtgt gggtaaagcg acagcttatc
    24181 ttgcggtaca actgaaaaaa acaccggaag cggctgctga gtttgctgca aagatgcagg
    24241 atgctaccgg aacggcgtca gaagacatga tggggctgtt cgacactatc cagaaggcgt
    24301 tttatctggg cgttgacgat accaacatgt tgtccttctt cactaaaacc agttctgttc
    24361 tgaagatggt gaacaaggac ggtcttcagg ctgcacagag ccttgccccc atcagcgtca
    24421 tgatggatca gatggggatg aacggggagt cggcaggtaa tgccctgcga aaagttatcc
    24481 agtccggatt aagcgttaag aaaatcaggg acgtcaataa aatcatggcc cgccagaaac
    24541 tcggggtaca gctcgatttt actgacggca aaggaagttt tggcggtctt gataacatgt
    24601 tcaggcaact ggcaaagctg cgaaaactga ccgacgttaa gcgaacaggt gtacttaagg
    24661 caatatttgg tgatgatgcc gaaacccttc aggtggtcaa tgcactaatc gataaaggaa
    24721 aggatggcta cgatcagatc cagcagaaga tgaataaaca ggccagcctg aataaacgtg
    24781 ttcaggccca gcttggtacg ctgtccaacc tgtgggaggc aatgacgggg accgcaacta
    24841 acggccttgc ggctattggc ggcgcatttt ctggtgacgc caaaaatatc acgcaatggc
    24901 tgggggagtt gggggagaaa ttcacgaagt ttgcggatga aaatccccgg gttattcgcg
    24961 gcgtcgtcgg gcttgctgcc ggtcttgcga ttctgaaact gggattgatg ggcgtgggca
    25021 gtgccatcag tatcgtcagc aggatcatgt cgatgacgcc gattggcatg attgcgacgg
    25081 cgattgccct ggctgcggga ttaattatca ctaactggga tgttgtcgga ccttatttta
    25141 agaaactctg ggaaaccatt agtccttatt ttgaggctgg ctgggaactt ctgaagaagg
    25201 tttttgcctg gtcgccgctg gggatggtga tcaataactg gggaccggtt gttaagtggt
    25261 ttcaggatat gtgggataag ctgaagccaa ttattgagtg gtttaccgac agttcaggtg
    25321 acacggtcga tgccattaac tcggcgcagt ggggcgcggg tgcttatgat gcttatggga
    25381 cgggaatacc ggcgcgggga tacatacctt atccggcggt ggatctggct cagtcaaaca
    25441 acgcctccga tgccacaggc ccgaatccct tcatgattaa caaagcttct gcgccaaaag
    25501 ttgatggtga gatcaaggtc tcttttgtga attcgcctcc gggtatgcgg gttatggaaa
    25561 cgcgatccag cggttttgat gtcagccatg atgttggcta tacgcgcttt ggcaggtaat
    25621 gaaaaattaa tctgttaatg agtcccactc cggtgggatt ttttatgtac ggagtttata
    25681 tgacgtggaa agacagactt ca
//
LOCUS       DADLTF010000046        25448 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.046, whole genome shotgun sequence.
ACCESSION   DADLTF010000046 DADLTF010000000
VERSION     DADLTF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.046"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            65..523
                     /locus_tag="JN749_001802"
     CDS             65..523
                     /locus_tag="JN749_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005083197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7368246.1"
                     /translation="MVNYAFDYLRSPGSLPLTTAATELSAIHGHSTSQYRLGEFYLHG
                     SDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKPDTRKAILWYKEAAEQG
                     YAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(533..1960)
                     /locus_tag="JN749_001803"
     CDS             complement(533..1960)
                     /locus_tag="JN749_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HAY7368247.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1957..2664)
                     /locus_tag="JN749_001804"
     CDS             complement(1957..2664)
                     /locus_tag="JN749_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7368248.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2822..3805
                     /locus_tag="JN749_001805"
     CDS             2822..3805
                     /locus_tag="JN749_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HAY7368249.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(3875..4357)
                     /locus_tag="JN749_001806"
     CDS             complement(3875..4357)
                     /locus_tag="JN749_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HAY7368250.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            4592..7174
                     /gene="leuS"
                     /locus_tag="JN749_001807"
     CDS             4592..7174
                     /gene="leuS"
                     /locus_tag="JN749_001807"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAY7368251.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7189..7770
                     /gene="lptE"
                     /locus_tag="JN749_001808"
     CDS             7189..7770
                     /gene="lptE"
                     /locus_tag="JN749_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HAY7368252.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            7770..8801
                     /gene="holA"
                     /locus_tag="JN749_001809"
     CDS             7770..8801
                     /gene="holA"
                     /locus_tag="JN749_001809"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAY7368253.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRTLFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8803..9444
                     /gene="nadD"
                     /locus_tag="JN749_001810"
     CDS             8803..9444
                     /gene="nadD"
                     /locus_tag="JN749_001810"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAY7368254.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9468..10079
                     /locus_tag="JN749_001811"
     CDS             9468..10079
                     /locus_tag="JN749_001811"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HAY7368255.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10339..10656
                     /gene="rsfS"
                     /locus_tag="JN749_001812"
                     /gene_synonym="rsfA"
     CDS             10339..10656
                     /gene="rsfS"
                     /locus_tag="JN749_001812"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAY7368256.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10660..11127
                     /gene="rlmH"
                     /locus_tag="JN749_001813"
     CDS             10660..11127
                     /gene="rlmH"
                     /locus_tag="JN749_001813"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAY7368257.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11158..13059
                     /gene="mrdA"
                     /locus_tag="JN749_001814"
     CDS             11158..13059
                     /gene="mrdA"
                     /locus_tag="JN749_001814"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HAY7368258.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13062..14174
                     /gene="mrdB"
                     /locus_tag="JN749_001815"
                     /gene_synonym="rodA"
     CDS             13062..14174
                     /gene="mrdB"
                     /locus_tag="JN749_001815"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HAY7368259.1"
                     /translation="MTANPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14185..15273
                     /gene="rlpA"
                     /locus_tag="JN749_001816"
     CDS             14185..15273
                     /gene="rlpA"
                     /locus_tag="JN749_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HAY7368260.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15412..16623
                     /gene="dacA"
                     /locus_tag="JN749_001817"
     CDS             15412..16623
                     /gene="dacA"
                     /locus_tag="JN749_001817"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HAY7368261.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16734..16997
                     /locus_tag="JN749_001818"
     CDS             16734..16997
                     /locus_tag="JN749_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HAY7368262.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17098..17739
                     /gene="lipB"
                     /locus_tag="JN749_001819"
     CDS             17098..17739
                     /gene="lipB"
                     /locus_tag="JN749_001819"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HAY7368263.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17998..18951
                     /locus_tag="JN749_001820"
     CDS             17998..18951
                     /locus_tag="JN749_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HAY7368264.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19160..20125
                     /gene="lipA"
                     /locus_tag="JN749_001821"
     CDS             19160..20125
                     /gene="lipA"
                     /locus_tag="JN749_001821"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HAY7368265.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="JN749_001822"
     CDS             complement(20226..20429)
                     /gene="tatE"
                     /locus_tag="JN749_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HAY7368266.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20558..21346)
                     /locus_tag="JN749_001823"
     CDS             complement(20558..21346)
                     /locus_tag="JN749_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HAY7368267.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            21439..21822
                     /gene="crcB"
                     /locus_tag="JN749_001824"
     CDS             21439..21822
                     /gene="crcB"
                     /locus_tag="JN749_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HAY7368268.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="JN749_001825"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21876..22085)
                     /gene="cspE"
                     /locus_tag="JN749_001825"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7368269.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22260..22820)
                     /locus_tag="JN749_001826"
     CDS             complement(22260..22820)
                     /locus_tag="JN749_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HAY7368270.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23409..24794
                     /gene="dcuC"
                     /locus_tag="JN749_001827"
     CDS             23409..24794
                     /gene="dcuC"
                     /locus_tag="JN749_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HAY7368271.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24835..25176)
                     /locus_tag="JN749_001828"
                     /pseudo
     CDS             complement(24835..25176)
                     /locus_tag="JN749_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT         6516 a         6322 c         6249 g         6361 t
ORIGIN      
        1 ttttatagtt gcagcaatta cctgtattag ccaacccaag aaaacgacgt tgcgtgataa
       61 agccatggtg aattatgcct ttgattattt aagatcacca ggcagtctac cgttaaccac
      121 ggcagccacg gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga
      181 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
      241 tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct
      301 gaaaggcctg ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc
      361 tgaacaaggg tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg
      421 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
      481 caaaaatgcg gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga
      541 agagatggag tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga
      601 tgacgcccag acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg
      661 gctcgacggt ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta
      721 atgcttccag tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt
      781 cttctgtcgg ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg
      841 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
      901 cgaccagtga acgccccagc gcagagagtt tttcatcgct gagtatttcg tataaatgaa
      961 aacgcgtacg ggcgctgtcg ccggggacat aaagtagcgg ttttccctga tcgttaacca
     1021 gatcacgcgc ttcaatcatc atcaccacta atgggtaata atcgacccga tgctgacggc
     1081 accattgcaa cattaaagca tccgcctggg tggagggata ctcgcgccag tagtaacaaa
     1141 gttctgccag caggctttcg ccttctccac aatagacgcg ttgtgcgtat tcataataac
     1201 gcgcatcttc attatcaggt tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg
     1261 ggattcccag gcggcttgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
     1321 cggggataat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
     1381 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
     1441 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
     1501 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
     1561 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
     1621 accagcgtaa ttcatcaatc tcttccatcg agcaataatt taattgctga atgaatcgct
     1681 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca
     1741 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
     1801 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg
     1861 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
     1921 tcgtttcctc tatatcgagg atcttccagc aatttttcat aagtcgctca tatccttaag
     1981 cgtttccggg catgtggggt agtcagccag tggttgccag gacaaggtag ggtaatacgc
     2041 atcatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
     2101 cgctaagaat tcgcgtaata aaggcagatg atcatcttcg ggagactgcc aatattgccg
     2161 accaaaggta taaccggcaa agtattgccg ccagttacta taataacgta gagcccgtag
     2221 atgaatacgc gacaggatcc atagactttc ttcttcactc aaccagttat taagtacgcc
     2281 cattcgactt aaaaaaccca tccggacata atcccatgct ttaattcctc cctcaccaca
     2341 gcattcggct gtgctggcga caaattgcgc ataagcctga ccttgctcat ttaattcagc
     2401 aagatagtca cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa
     2461 ccgagcggca tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc
     2521 atcaattccc catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttggggga
     2581 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
     2641 tattttttgc gaccccatgt ccatataatt tatccttaaa cattcattta atgaatgcaa
     2701 actacatttc gagactcagt tatttatact tttctgacgc aacataatac gcataaaata
     2761 aggattacgc aaaaatggaa cacataaaaa tataatatat tttatctaca accataagga
     2821 aatgataata atttttacgt caagttgctg cgataattta tcaatagatg agattatcga
     2881 acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga
     2941 tagcgcaatt gactctccgg acgacgaaaa ggccttttac tggctgaagc tggccgctga
     3001 gcaaggtcat tgtgaagcac agtattcctt agggcaaaag tataccgagg ataaaagccg
     3061 tcataaagat aatgagcaag ccatcttctg gctgaaaaaa gctgccctac aaggccatac
     3121 tttcgcttcc aacgcccttg gctggatact ggatcgtgga gaagacccca actataaaga
     3181 agcggttacc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct
     3241 tggatggatg tacagaaatg gcaacggagt cgcacaagac tatacgctgg catttttctg
     3301 gtacaaacaa gctgcattac aaggccatag ttacgcacaa gacaatctgg ccgatcttta
     3361 taaagacgga gaaggcgttg ctcaaaacaa gacactcgcc gcattctggt atttgaaaag
     3421 cgcacagcag ggtaatcggc acgcccagtt tcaaattgca tgggattata acgctggcga
     3481 aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcagga
     3541 aagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa
     3601 agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc
     3661 ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca
     3721 ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat
     3781 tcgtgagact gaagctttac tgtagaaata aaaacaaggg ccggggaatt tcattctgcc
     3841 catattctct ttattgctat gcgaaatcag actattacgg ctcaaacggg cgtctctgga
     3901 actggtcata accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat
     3961 ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa
     4021 ccccatgatg attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga
     4081 tgtctgccag ctcctgccac aagctttctt taatcacccg cataaagacg ctgtcagatt
     4141 cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt ctgacagctc
     4201 gcgtcagctc atcgatctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg
     4261 cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ttcagtgacg
     4321 cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa
     4381 ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt
     4441 aaattaatgt acgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt
     4501 gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt
     4561 agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat
     4621 agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga
     4681 gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat
     4741 gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgttaccagc gcatgctggg
     4801 caaaaacgtt ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc
     4861 ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa
     4921 ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc
     4981 ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata
     5041 taaaaagact tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca
     5101 agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca
     5161 gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga
     5221 tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt
     5281 ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc
     5341 ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa
     5401 agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt
     5461 tgccgaagct gaaatggcga cgctggagaa aaaaggcgtc gatactggct ttaaagctgt
     5521 tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta
     5581 cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc
     5641 taaatacggc ctgaacatca aaccggttat cctggcggct gacggctctg agccagatct
     5701 ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgagt tcaacggtct
     5761 tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga
     5821 gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc
     5881 gccgattccg atggtgacgc tggaagacgg taccgtaatg ccaaccccgg acgaccagct
     5941 gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga
     6001 tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt
     6061 cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga
     6121 aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg
     6181 tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga
     6241 tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct
     6301 ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga
     6361 tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca
     6421 tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc
     6481 gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc
     6541 tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct
     6601 gaaacgtgtc tggaaactgg tgtacgagca cacagcaaaa ggtgatgttg cggcactgaa
     6661 cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaactatcgc
     6721 taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat
     6781 ggagctgatg aacaaactgg cgaaagcacc gaccgatggc gagcaggatc gcgctctgat
     6841 gcaggaagca ctgctggccg ttgtccgtat gctcaacccg ttcaccccgc acatctgctt
     6901 cacgctgtgg caggaactca aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc
     6961 tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt
     7021 ccgtgccaaa atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg
     7081 ccaggaacat ctggtagcaa aatatcttga tggcgttact gtacgtaaag tgatttacgt
     7141 accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg
     7201 gcaacattgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt
     7261 gacaccacgc aggttccttc cactatgaag gtcatgatcc tagactcagg cgatccgaac
     7321 gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat
     7381 aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa
     7441 gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg
     7501 aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagcgccaa agtcttccgt
     7561 tcattcttcg ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta
     7621 aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct
     7681 gcggatattc gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct
     7741 gcacgcgtct ccaccatgct gggtaactga tgattcggtt gtacccggaa caactccgcg
     7801 cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat
     7861 tgcaggaaag ccaggacgct gttcgccagg tagctgcggc acaaggattc gaagaacacc
     7921 acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta
     7981 tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg
     8041 cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga
     8101 tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg
     8161 cgaatcgcag cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctggg
     8221 ttgctgtgcg cgcaaaacag ctcaacttag aactggatga cgcggcgaat caggtgctct
     8281 gctactgtta tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc
     8341 tctggccaga cggcaaactg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc
     8401 atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc
     8461 atattcttca gcaactgcgt ctggaaggca gcgagccggt tattttgttg cgcacattac
     8521 aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgta
     8581 cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa
     8641 atcgcttaag tcagccgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca
     8701 ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt
     8761 tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct
     8821 ctgtttggcg gcacctttga tccggtgcac tatggtcatc taaaacccgt ggaaacgctg
     8881 gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt
     8941 ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac
     9001 aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgca
     9061 caaacactga aagagtggcg gcaggaacaa ggaccggacg tgccgctggc gtttattatt
     9121 ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat
     9181 gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac
     9241 cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc
     9301 ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa
     9361 cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtatt gacttacatt
     9421 aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt
     9481 taattcgtca tggcgaaaca caagcgaatg tcgatggtct gtacagcggt catgcgccca
     9541 cacccctgac cgcacgcggt attgagcaag cgcaaaatct gcatacactg ctagatgatg
     9601 tttcctttga cctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9661 ttctcagtga ccgccagctc cccgttcata tcatccctga actcaacgaa atgttttttg
     9721 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9781 cgtggtgcaa tgactggcag catgctatcc ccacgaacgg tgaaggattt caggcatttt
     9841 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaata tcagcactat cagaatattt
     9901 taatcgtcag ccatcagggc gtactgagtc tgttaattgc tcgtttaatt ggcatgcctg
     9961 ccgagtctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
    10021 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac
    10081 ttttctgttt ttttacgggt aagccgcaac gaccattgac agccccgggc aggctgatat
    10141 tctccgcagc cagacttttt ccgccagaca cgactttgta gaaattgttt tacaaaaatg
    10201 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10261 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10321 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10381 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10441 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10501 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10561 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10621 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10681 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10741 ccgaaagata tgcccttcga gctgattgaa attccggccg gcaaacgcgg caagaatgcg
    10801 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10861 cgcattgtca tcctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10921 ctggaacgct ggaagctgga tggtcgcgac gtaagtctac tgattggcgg gcctgaaggg
    10981 ttgtcgcctg cctgtaaagc ggcggctgag cagagctggt cgctgtcggc gcttaccctc
    11041 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    11101 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11161 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11221 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11281 ctgcaaattg ttcgctttac cgactaccag acccgctcta atgaaaaccg cattaagctg
    11341 gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc tctggccctc
    11401 aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg
    11461 ctggacgctt tgcgcagcgt ggtagatctg accgatgacg atattgctgc attccgaaaa
    11521 gagcgcgcac gttcacaccg tttcacctct attccggtga aaactaacct gaccgaagta
    11581 caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat
    11641 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggata tgtgtcgaaa
    11701 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11761 acgcatgata ttggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11821 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11881 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    11941 attgaaacgc tgctggcggg tagccgcgca gctgtggttg ttaccgatcc gcgtacaggt
    12001 ggggtgctgg cgctggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc
    12061 tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc
    12121 acacaggggg tttatcctcc cgcgtctaca gttaaaccct atgtggcggt ttcggcatta
    12181 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca
    12241 gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12301 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12361 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12421 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12481 ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc
    12541 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12601 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12661 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12721 gttgctaacc gccctaatgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt
    12781 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12841 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12901 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    12961 acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaacac cgatctgcct
    13021 gcggaaaatc cagcggttgc cgcagcggag gaccattaat catgacggct aatccgaata
    13081 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatt ttactggcat
    13141 tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13201 agcgtaaaat cggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13261 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13321 tggcggtaga tgctttcgga gccatctcta aaggcgctca acgctggctg gacctcggta
    13381 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13441 ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga
    13501 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13561 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13621 cagtagtgct ggtagcggcg ttcattccga ttctatggtt cttcctgatg catgattacc
    13681 agcgccagcg cgtaatgatg ctcctagacc cggaatcaga cccactcggc gcgggctatc
    13741 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13801 acggcactca gtcacagctt gaatttctcc ccgaacgcca tactgacttt atcttcgcgg
    13861 tactggcgga agagctggga ttagtgggca ttctgattct gctcgctctc tacattctgc
    13921 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    13981 ctggtggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    14041 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa
    14101 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14161 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14221 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14281 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14341 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14401 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14461 aacctgacgg cctctggcga agctttcgat ccgacaaagc tgacggcggc ccatccaacg
    14521 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14581 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14641 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14701 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttat
    14761 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14821 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gaccggcgcg
    14881 ccggtaacca gtagcggttt cctcggcgct caaacgacct tagcgcctgg tgtactggaa
    14941 ggcagcgaac cgacgcctgc gccacagccc gttgttacag cttcgtcgac aacgcctgca
    15001 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagcgg caactttatg
    15061 gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15121 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15181 ggcccattcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15241 caattacagt catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15301 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15361 gctatagtag ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc
    15421 attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt
    15481 atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag
    15541 atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag
    15601 aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc
    15661 ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac
    15721 gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc
    15781 atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct
    15841 tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac
    15901 agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat
    15961 gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt
    16021 gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc
    16081 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
    16141 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
    16201 ttgatttctg cggtgatggg tggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
    16261 ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag
    16321 ttcgcctcag aaccggtttg gtttagtgat tctgatcgcg cttcgttagg ggttgataaa
    16381 gacgtgtacc tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
    16441 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
    16501 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
    16561 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
    16621 taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
    16681 actcccacct tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa
    16741 ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg
    16801 cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact
    16861 acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca
    16921 acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg
    16981 tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactctcctc
    17041 tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg
    17101 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccgg
    17161 gctatgcatg aattcaccga tacccgcgat aatagtaccc ttgatgaaat ctggctggtc
    17221 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
    17281 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga
    17341 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
    17401 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
    17461 cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta
    17521 cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
    17581 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
    17641 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
    17701 ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa
    17761 tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta
    17821 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt
    17881 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
    17941 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
    18001 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
    18061 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat
    18121 gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt
    18181 cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag
    18241 ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag
    18301 tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt
    18361 gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact
    18421 cactatccgc agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt
    18481 cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat
    18541 catgttctgt tcaccgacaa tatggtgtta atttgccgca agggaaatcc actactctct
    18601 ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaaggg
    18661 caaaatttca gcggtctgcg gcagagagtt caagagatgt ttccggaccg gcaaatcaat
    18721 ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg
    18781 attattccca gccgttttta taacctgttt agtcgttgct ggccgctgga aaaactgcct
    18841 tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg
    18901 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
    18961 gttacagcca gatcacagtg cagaacgata aaatggcaca atccaacaac aattttacat
    19021 tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat
    19081 tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg
    19141 caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg
    19201 cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct
    19261 gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg
    19321 catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc
    19381 taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg
    19441 tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa
    19501 tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac
    19561 ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccaa cactttgcgg attgtattac
    19621 tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgcgg
    19681 tcgaatggat cgtgctctgg atattctgac tgcaacgccc ccagatgtgt tcaaccataa
    19741 cctggaaaac gtaccgcgta tttaccggca ggtacggcct ggcgcagatt acaactggtc
    19801 gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct
    19861 gatggtgggt ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg
    19921 tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc
    19981 ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat
    20041 gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca
    20101 ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa
    20161 acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg
    20221 ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc
    20281 gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct
    20341 ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc
    20401 agcagtttgg taatactaat ctcacccata gataccttct tgacatataa ccggcaacag
    20461 aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat
    20521 ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa
    20581 agcgacggtt gtttaagatg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca
    20641 cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat
    20701 ctataatccg gctttgcccg atatttttgt tcccgcactc ccccgccgcc accatataac
    20761 aggtggtatc cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc
    20821 cacgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaaagcc tgtgccagcg
    20881 ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccttccacct
    20941 ccagtaacgg agctatttca ttaccagcat caacatggcg tgattcctga atggcaaatg
    21001 catcatagag atgcagtttg gcataacggg cgacgatgtt tcctgcctga agtgccacca
    21061 gcatattcca tgcgcgcccc ggcgttgaag gaagatgaat cgtcagaatt gtcgtcatca
    21121 tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg
    21181 atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacaa
    21241 acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct
    21301 tttcccacac agatgtaaca gcaaactgtc ctgcagcaac cagcattgcc acctcctgtc
    21361 agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc
    21421 aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg
    21481 ggaagcgtag cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg
    21541 ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg
    21601 ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt
    21661 ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc
    21721 cgctttggct gggcattact gaacgttttc gtcaaccttc tggggtcttt tgccatgacc
    21781 gcactggcat tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaacccgc
    21841 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21901 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21961 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    22021 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    22081 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt tatcagtaca
    22141 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22201 ttttgatgtt gcttatttta gttattgatt ttaaagaagt tactaaaact tcatttgcct
    22261 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22321 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22381 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22441 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22501 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22561 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22621 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22681 tgttcaggct gttgccaggt ttgtgcaata ttttctctaa acgttgtcat cctctcatct
    22741 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22801 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22861 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22921 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22981 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    23041 atgcaatcaa ggaaaaatta ttttattttt ttaacgagat acaacaatca tcttaacgaa
    23101 gtatataata tatatcgtca acccatgctc atattagatt attttttgag cgcacagccg
    23161 cagctaacac cattgcaatt aacaaatttg catcaatcca ccatcaattt acacacatta
    23221 ttatgtgata attgccaacc gctaaatatg cgttttgtta tctatgtata aaaacagcaa
    23281 cttcaatgtc ttaatggcag tttttcttga ttttaatcag cattcatcgc caatttattg
    23341 ggcatatttt ttccttaagc tttaggaagt ttttattatt tactttgggc ctggagacag
    23401 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23461 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23521 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23581 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23641 acctcggcat gatgattatg atgctgtgtg gctttgccgc ttacatgacc catatcggcg
    23701 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23761 tgctgatgat tgccgcctat tttgttgctt gtctgatgtc actggccgtc tcttccgcaa
    23821 ccggtctggg tgttttgctg atggcaaccc tgtttccggt gatggtaaac gttggtatca
    23881 gtcgtggcgc agcagctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23941 caggggatgt ggtgctggcg gcgcaggctt ccgaaatgtc gctgattgac ttcgccttca
    24001 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    24061 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24121 tcaccaccac tgcccccgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24181 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24241 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24301 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24361 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24421 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24481 ccatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24541 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24601 tgcaggcgtc aaacctgggt cgtaccctat cacccgtttc tggcgtagtc gttgcggttg
    24661 ccgggatggc gaaaatctca ccatttgaag tcgtaaaacg cacctcggtg ccggtgcttg
    24721 ttgggctagt gattgttatc gttgctacag agctgatggt gccaggaacg gcagcagcgg
    24781 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24841 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24901 atggcggctg gcgcaatatt caagataacg cctggcagtg gtgcggctga tggtcagtgc
    24961 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    25021 gtttagcgtc aaggggtcaa tgccggtcgg cagttcgtct ttgggttcac cgcgagcata
    25081 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25141 tttacactgg cggaaacggg ttagcgtttg ccccagtgta ttgattcact tgaagtacga
    25201 aaaaaccggg agaacattgg attattcggg atctgatggg attagatttg gtggggcttg
    25261 caagcctgta gtacaaattt taggcgttaa ccaatgaaac gcgaaagata gtaaaaaatt
    25321 gcttttgttt cattgaaaaa acgaaaaaca aaaacactat aaatcatttc aatcatagct
    25381 tcaaaaaacg ttcaaaaccg ataacaacca ggctgtcacc aaatgactca tatcacaaat
    25441 cagctcat
//
LOCUS       DADLTF010000047        25429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.047, whole genome shotgun sequence.
ACCESSION   DADLTF010000047 DADLTF010000000
VERSION     DADLTF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.047"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..105)
                     /locus_tag="JN749_001829"
     CDS             complement(<1..105)
                     /locus_tag="JN749_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HAY7368272.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQV"
     gene            complement(175..1164)
                     /locus_tag="JN749_001830"
     CDS             complement(175..1164)
                     /locus_tag="JN749_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7368273.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1961..2464
                     /locus_tag="JN749_001831"
     CDS             1961..2464
                     /locus_tag="JN749_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HAY7368274.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2544..2795)
                     /locus_tag="JN749_001832"
     CDS             complement(2544..2795)
                     /locus_tag="JN749_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HAY7368275.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2910..2996)
                     /gene="azuC"
                     /locus_tag="JN749_001833"
     CDS             complement(2910..2996)
                     /gene="azuC"
                     /locus_tag="JN749_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HAY7368276.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3259..3582
                     /locus_tag="JN749_001834"
     CDS             3259..3582
                     /locus_tag="JN749_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368277.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLH
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3753..4250
                     /gene="ftnA"
                     /locus_tag="JN749_001835"
     CDS             3753..4250
                     /gene="ftnA"
                     /locus_tag="JN749_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HAY7368278.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4287..4526)
                     /locus_tag="JN749_001836"
     CDS             complement(4287..4526)
                     /locus_tag="JN749_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HAY7368279.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4716..5927
                     /gene="tyrP"
                     /locus_tag="JN749_001837"
     CDS             4716..5927
                     /gene="tyrP"
                     /locus_tag="JN749_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HAY7368280.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSMPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5989..6654)
                     /gene="yecA"
                     /locus_tag="JN749_001838"
     CDS             complement(5989..6654)
                     /gene="yecA"
                     /locus_tag="JN749_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HAY7368281.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6850..6936)
                     /locus_tag="JN749_001839"
     tRNA            complement(6850..6936)
                     /locus_tag="JN749_001839"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6900..6902),aa:Leu,seq:taa)
     gene            complement(6949..7022)
                     /locus_tag="JN749_001840"
     tRNA            complement(6949..7022)
                     /locus_tag="JN749_001840"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6988..6990),aa:Cys,seq:gca)
     gene            complement(7304..7852)
                     /gene="pgsA"
                     /locus_tag="JN749_001841"
     CDS             complement(7304..7852)
                     /gene="pgsA"
                     /locus_tag="JN749_001841"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAY7368282.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7909..9741)
                     /gene="uvrC"
                     /locus_tag="JN749_001842"
     CDS             complement(7909..9741)
                     /gene="uvrC"
                     /locus_tag="JN749_001842"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAY7368283.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9738..10394)
                     /gene="uvrY"
                     /locus_tag="JN749_001843"
     CDS             complement(9738..10394)
                     /gene="uvrY"
                     /locus_tag="JN749_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HAY7368284.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10690..10866
                     /locus_tag="JN749_001844"
     CDS             10690..10866
                     /locus_tag="JN749_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368285.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10853..11077
                     /locus_tag="JN749_001845"
     CDS             10853..11077
                     /locus_tag="JN749_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HAY7368286.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11110..11730)
                     /gene="sdiA"
                     /locus_tag="JN749_001846"
                     /pseudo
     CDS             complement(11110..11730)
                     /gene="sdiA"
                     /locus_tag="JN749_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11960..12712)
                     /gene="yecC"
                     /locus_tag="JN749_001847"
     CDS             complement(11960..12712)
                     /gene="yecC"
                     /locus_tag="JN749_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HAY7368287.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12709..13377)
                     /gene="tcyL"
                     /locus_tag="JN749_001848"
     CDS             complement(12709..13377)
                     /gene="tcyL"
                     /locus_tag="JN749_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HAY7368288.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13392..14378)
                     /gene="dcyD"
                     /locus_tag="JN749_001849"
     CDS             complement(13392..14378)
                     /gene="dcyD"
                     /locus_tag="JN749_001849"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HAY7368289.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14483..15283)
                     /gene="tcyJ"
                     /locus_tag="JN749_001850"
     CDS             complement(14483..15283)
                     /gene="tcyJ"
                     /locus_tag="JN749_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7368290.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15371..15922)
                     /gene="fliZ"
                     /locus_tag="JN749_001851"
     CDS             complement(15371..15922)
                     /gene="fliZ"
                     /locus_tag="JN749_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HAY7368291.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15968..16687)
                     /locus_tag="JN749_001852"
     CDS             complement(15968..16687)
                     /locus_tag="JN749_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HAY7368292.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVCHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16852..18504)
                     /locus_tag="JN749_001853"
     CDS             complement(16852..18504)
                     /locus_tag="JN749_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HAY7368293.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTVGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18770..20176
                     /gene="fliD"
                     /locus_tag="JN749_001854"
     CDS             18770..20176
                     /gene="fliD"
                     /locus_tag="JN749_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HAY7368294.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKVTLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20201..20611
                     /gene="fliS"
                     /locus_tag="JN749_001855"
     CDS             20201..20611
                     /gene="fliS"
                     /locus_tag="JN749_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HAY7368295.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20611..20976
                     /gene="fliT"
                     /locus_tag="JN749_001856"
     CDS             20611..20976
                     /gene="fliT"
                     /locus_tag="JN749_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HAY7368296.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21054..22541
                     /gene="amyA"
                     /locus_tag="JN749_001857"
     CDS             21054..22541
                     /gene="amyA"
                     /locus_tag="JN749_001857"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HAY7368297.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDCIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22575..22988)
                     /locus_tag="JN749_001858"
     CDS             complement(22575..22988)
                     /locus_tag="JN749_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7368298.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23175..24380
                     /gene="yedE"
                     /locus_tag="JN749_001859"
     CDS             23175..24380
                     /gene="yedE"
                     /locus_tag="JN749_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HAY7368299.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24377..24610
                     /gene="yedF"
                     /locus_tag="JN749_001860"
     CDS             24377..24610
                     /gene="yedF"
                     /locus_tag="JN749_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HAY7368300.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24717..25387
                     /locus_tag="JN749_001861"
                     /pseudo
     CDS             24717..25387
                     /locus_tag="JN749_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         6333 a         6486 c         6042 g         6568 t
ORIGIN      
        1 aacctgaccg gcatagcagt caaaactaat atccgtcagc gccttaacgc cgggaaacgt
       61 tttaccgatg ccgcgaaatg agagatacgg ggtagactgt tgcataacgt ctccgtgaat
      121 cactggtcgt tcacaccccc tctgcatcat gcagagggga atttttccgg caaattactt
      181 accgcctaaa ccttttttct ccagttcttc tttaaagttg tcacgcgtga tcagtaccac
      241 gtctgtaact tcggtaaatt ttggcgtttc aacgtctttt gctacccagt tgtaaagcat
      301 ttcgctggat ttatagccat gtacgtccgg gcttggcagc agggaaccgt agaagccggt
      361 tgcctgtgct ttagacagtt cgcttactgc atccacaccg ttaataccaa taccgatgat
      421 atcggctgct ttaaagccct gaccttcggt tgcacgtacg ccgccaagta cggtgctgtc
      481 gttcataccg acgatcagcc aatgtttaac ttccggatgt tgaaccagca ttgagttggc
      541 agcgtcaaat gcccccggga tgtcgttaga tttggtaggt acctgataaa tttgtttttc
      601 cgggaatcct gcagctttca gtgcgtccat agaacccgtt gtacggcggc gggcggtatc
      661 cagttcgttg gcggtaatcg ccatcactgc gctttctttg acatcccagc cacgtttctg
      721 catctcttta tacagttcct ggccctgacg ttcgccaatt ttagtcgccg ccatcatcac
      781 cagaggaacg gtatccattg gcttaccttt ggcgttaaca aactggtcat ccactgcaat
      841 gactttcata tcgtagccac gcgctttcgc gacgatggca gagccgagtt tcgggtccgg
      901 agtacaaata acgaaacctt ttgcgccact ggctgccagg ctgtcgatcg cgttcaatgt
      961 tttttcgcca tccggcacgg caatcttaat aacctcaaac cctaaatcct tcccggcttt
     1021 atcggcaaac ttccattcgg tctggaacca cggctcttcc ggttgcttca ccagaaaacc
     1081 gagcttcagg ttctccgcca tagcggattg tgacataacg gctgccagac caatggctgc
     1141 cagggcttta gtaaatttgt gcatggttct ctccagcttt agtgtcgttt tgtgtagggc
     1201 aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag cattaccttt tatctaaaag
     1261 ataagtgacc gtgttgacat agttttagcg agaaattaat tctccatagg agagcaatat
     1321 cacatcgcag aattacagtg agaacgtgca taaatttagc gggaaaagac ataaggaaaa
     1381 gccaatttgt cagacaaatt gttgaatgca cagcagatta atccataaga ttagcctgga
     1441 aatccttgtt gtcttcggta cccatgcggg atgtcttctt tttaaccagt caataggccg
     1501 cattacctgg cgttgagttt ttgaaatggt gtaataacca caactcaaag atgtggaaaa
     1561 tgcgcgtcat tcatttcgcc attaattatc actgtgctca ttaattaaca gaacacgtat
     1621 aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa aatcatcact tcatcatgaa
     1681 ttcaaattca tcgattaata tcaacaagat acaaaaagca ctatcattaa aattcattgc
     1741 atttacattg atttcaccaa tgaaatgtaa aaatatataa acttgatgat ttaagcattt
     1801 tcttataacc gttcagacgt tattcttatt tcagatcatc gtcagaattg actccacgat
     1861 cacatttcgg accggcagaa aggaattatt ctgcaaacag taattatggt gttttgattt
     1921 atcttgcacc tctccacttc tggatataag gatattaggt atggcaaccg ctggaatgct
     1981 tctcaaactc aactctcaaa tgaaccgcga gttttacgca tccaatctct accttcacct
     2041 gagtaactgg tgttctgaac agagtctgaa cggcaccgcc actttccttc gcgcccaggc
     2101 acagagtaat gtgacccaaa tgatgcgcat gtttaacttt atgaagagtg tcggcgctac
     2161 ccccatcgtt aaagccattg atgttccagg tgaaaaactg aactctctgg aagaactgtt
     2221 ccaaaaaacg atggaagaat acgagcaacg ttcaagcacg ctggcgcagt tagccgatga
     2281 agcgaaagaa ctgaatgatg attcaaccgt caatttcctg cgcgatctgg aaaaagaaca
     2341 gcagcatgat ggcctgttgc tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc
     2401 gggtatgtgc cctgtgcaga ccgaccaaca tgttctgaat gtcgtgtcac accagctgca
     2461 ttgatcatac atcggcgcta atacattgcg ccgatgaagt ttttgagaaa ccgctgcctc
     2521 atctgtttga agcagcggtt tttttaatgg gattcaccct gtggggtaaa cttgagttca
     2581 atcagcgcga tggctttttg gattgcccgc atggtgaccg ggtctgcggc agcgggatga
     2641 tgagtaaagt cgatattctt cagctgactg gacatttttt cacgaacttc aacgggcgcg
     2701 attacatcga gaacatccag aatttgtttg ataaccaact ggcaagctac cacatcagaa
     2761 accagttcct gatcggcatt cagcggctgg gacatcgtaa actcctgata gcattttgaa
     2821 agccgttata gtagcggctt cacatcttca gcgatagtca catccaccgt catcaggaca
     2881 caaaaaaacc tgccggagca ggttttttgt tatcggaaca tattgcctgg cggtacgtct
     2941 ttgaacgtct tgcaatagtt attgaacata cttttcagga ttttgcgcag tttcatcgcg
     3001 gcactccgac catttgttat acaggtgtta ttgtctttgc gcttataata tgacaaccat
     3061 cacaaaaatc aatctttatg tgatacaaat cacataaata cccctttaat gttataaaaa
     3121 tgataatcaa aaaacagccc ccctatttct gacacctaca gatggcaaga aatagcgcct
     3181 gccaggcatc ttttccggcc attgtcgcag cgctgtaacg cgtaaaatag tgctttctct
     3241 tactcttctg gctggaccat gagacttctg attctgactc tttcattaat aacgctggcg
     3301 gggtgtacgg ttactcgcca ggcccatgtg agcgaagttg acgcggcaac aggtattgta
     3361 cggttggttt atgaccaggc ttttttgcat catgcccata ccgatcgtta tgtcagtcgc
     3421 gggatcgccg atcgcgcatg ccagcaggaa ggctataccc acgcgatccc ctttggtcag
     3481 ccagtaggca actgtagcct ttttgcgggt tccttatgtt tgaatacgga attcactttg
     3541 tcatatcagt gccatcattc cgccttccct gtctttctct aaatatttta atcagcgagg
     3601 tgatcttcgc tgattaaaga aatagcggta atgcgtttta ttcccattcg tatttttaat
     3661 aattgaagtt tatattttac cttttgcaaa taataaaata acaaattata gtgacgccac
     3721 agcaacaaat ataacctttg tggagcacta tcatgctgaa accagaaatg attgaaaaac
     3781 ttaatgagca gatgaacctg gaactgtact cttcactgct ttatcagcaa atgagcgcct
     3841 ggtgcagcta tcataccttc gaaggtgctg ccgcgttcct gcgccgtcac gcccaggaag
     3901 agatgacgca tatgcagcgt ctgtttgatt acctgactga taccggcaat ttaccgcgta
     3961 ttaataccgt tgaatctccg tttgctgaat attcctcact tgatgaatta ttccaggaaa
     4021 cctataaaca cgaacaatta atcacccaga aaattaacga actggctcat gctgcaatga
     4081 ccaatcagga ctacccaaca tttaatttcc tgcagtggta tgtttctgag cagcatgaag
     4141 aagagaaact gttcaaatcg attattgata aattaagcct ggcaggcaaa agcggcgaag
     4201 gtctgtattt tatcgacaaa gaactctcta ccctcgacac acaaaactaa tgctctccgg
     4261 cggcaggttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg atcggtcgaa
     4321 aaaccttctt ctgaaggtat aaatttgcct tttgctgcca gaaacgctac cagctctccc
     4381 gctgtcatcc cttctgccga gcacgtgtga aaacgtgcct gttcaccaaa acgcgcttta
     4441 atcgcagctt ccagactggc atgcgtatat tgctcgcctg attcaatcat catatttaac
     4501 acttcatgac cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag cgtagcgatt
     4561 accgcttatg aagactttgc gccagcgcag gactgaatgc ttttattgta catttatatt
     4621 tacacaatat gtaacgtcga tttgacgaag cagccgttat gccttaacct gcgccgcaga
     4681 tatcactcat aaagatcgtc aggacagaag aaagcgtgaa aaacagaacc ctgggaagtg
     4741 tttttatcgt ggcgggaacc acaattggcg caggcatgct ggcaatgccg ctggctgcgg
     4801 ccggtgttgg ttttagcgtt acgttaatct tgttgattgg gctttgggcg ttgatgtgct
     4861 acacggcgct attactgctg gaggtgtacc agcatgttcc ggcagatacc ggtctgggca
     4921 cgctggcaaa acgctatctg ggacgctacg gtcaatggct gacgggcttc agtatgatgt
     4981 tcttaatgta tgctctgact gcagcataca tcagcggtgc cggtgaattg ttggcctcca
     5041 gcatcagcga ctggacaggt atttcaatgt cggcaaccgc tggcgtgctg ttgttcactt
     5101 ttgttgccgg tggcgtggtt tgtgttggaa catcgctggt cgatttattt aaccgttttc
     5161 tgttcagcgc caaaattatt tttctggtgg tgatgctggt attgctgcta ccgcatattc
     5221 acaaagtgaa tcttttaacc ctgccgttgc aacaggggct ggcactgtct gcaatcccgg
     5281 tgatttttac gtcgtttggt tttcacggta gcatgccgag tattgtcagc tatatggatg
     5341 gcaacattcg taagctacgc tgggtgttta taatcggtag tgccatcccc ctggtggcat
     5401 atattttctg gcaggtggcg acgcttggca gcattgattc aacaaccttt atgggattgc
     5461 tggctaatca tgctggatta aacgggctgt tacaggcgtt acgcgaaatg gtgccctctc
     5521 cgcatgttga gctggcggtg catttatttg ctgatttagc cctcgccacg tcatttctcg
     5581 gcgttgcgtt aggcttattt gattatctgg ctgatttgtt tcagcgttca aataccgttg
     5641 gtggacgatt gcaaactggt gcaattacct ttctgccgcc gttggcgttt gcactgtttt
     5701 atccacgagg atttgtgatg gcgctgggtt acgccggtgt ggcgctggcg gtactggcat
     5761 tgattatccc ttcactattg acctggcaaa gcagaaagca caatcctcag gcgggttacc
     5821 gggtcaaagg tggtcgtccg gcgctggtgg tggtgtttct ctgtggtatt gctgtgattg
     5881 gcgtgcaatt tttgattgcg gcagggttgt taccagaagt ggggtgatca gatagcctca
     5941 aattccttat tgggtgccag aattaacgct gacacccaat gtgacctctt aatgcaggca
     6001 gcactgctta aatttcttac cactgccgca cggacaagga tcgttacgcc ccggtttctc
     6061 ttctgctttg atcggttgct gaacagcttt ttcctgcgga tgcgccatcc agtatgcatg
     6121 taaatcaagc gccgccagtc gaatggcatc tacgctctct tcaaacgctt ctggcgacat
     6181 tttctctacc cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct ccagcgctgg
     6241 ttttaaagaa tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca tatagccaaa
     6301 gcaccactcc tcaacaatcg tcagctcgct gccatcaact tctcgcaagc cgaataacgg
     6361 ctcaaactgc tccgggaatt cgttcagacg ctctgcggta tcggccatat gttgaaaagc
     6421 caggttcata aagcgcgtca tctctttctc tgacgcccaa cgcggcacat agtcagcccc
     6481 accccacacg gcaaccagcc actgttctgg ttcaatctct tgcggagaac tcaacaccgc
     6541 cgtcaataaa ccgtccagct ccgccacatc aaggatggcg tggtcagtgt tgtatttggt
     6601 cagaatatcg tccagccatt ccaactcact ttcgtttaac ggtcccgttt tcatacgctt
     6661 ttccttgtgg atctcaactc gccagcacct atcttacatg ccggtccgta tcagagatac
     6721 tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca agttgcaggc
     6781 acaaaaaaac cacccgaagg tggtttcacg acactgctta ttactttgat tttattctta
     6841 tctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc cgagggattt
     6901 taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt
     6961 ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg
     7021 ccaaattctt caggcctttc agccagacat ccgcttgacg ccgatgtctt ttaaactgga
     7081 gcgggaaacg agactcgaac tcgagacccc gaccttggca aggtcatgct ctaccaactg
     7141 agctattccc gtattcatca agcaatcagt taatcacttg attttattat cgtctggcaa
     7201 tcagtgccgc cgttcgatgc gttgcattct acttacctgg cgcgatgagt caacgatatt
     7261 tttcaccact tttgatcgtt tgctgaaaat tacgccgaaa cgatcactga tcaagcaaat
     7321 ctgcacgcgc agcgctcaaa tattgcaaca ttgaccacag agtcagtacc gcagccacaa
     7381 agaaaagtgc aataccggcg tactcaaccc aaatgttcgg acgccacagc agccaggcca
     7441 acgccaccat ctgggcagtg gttttcactt tcccaatcca ggagacggcc acgctactgc
     7501 gtttacccaa ctccgccatc cattcgcgta gcgcagaaat aataatttca cgggcgatca
     7561 tcgttgccgc cggtaatgtc acccaccagc tgtgataatg ctcggttacc agcaccatgg
     7621 cgatagccac gagaacttta tctgccacag ggtcaaggaa agcaccaaac cgggtactct
     7681 ggttccagcg gcgtgccaga aaaccatcga accagtcagt caccgccgcg acgcagaaaa
     7741 tgagcgcggc ggcaaacggc gaccaggtga caggcagata aaagaccaat acaaagaatg
     7801 ggataaggat gacacggaac agtgtaagca acgtagggat attaaattgc ataatgacgg
     7861 gtaactatct gttgtcagta agattacccc tatgttgcta cagagacatc aatgtttcaa
     7921 cgaccagaag atcttttctg ccagaccttg cgaaataccc ggcacttttg caatttcctc
     7981 gacgctggcg ttacgtaaac cttgcaaacc gcccatatat ttcaacaaca tttgccgacg
     8041 ttttggcccg acgccttcaa tggtttccag ggaactggta tttttgacct tcgcccgttt
     8101 tttacggtgc ccgccaatcg cgtgatcatg tgattcatcg cgaatatgct ggataacatg
     8161 cagcgcgggt gaatctggcg gcaaactaaa tccctcacct tccggctcaa agaacagcgt
     8221 ttccagtcca gccttacgat ctgctccttt ggcaacgcca agtagcagcg gatgattttt
     8281 atcccatgag acatccagtt cggcgaagac atttttcgcc tgcgcaagct ggcctttgcc
     8341 gccgtcgata aggatcacat ccgggatctt actgtcgtca atggctttac cataacgccg
     8401 acgcagcacc tgattcatcg ccgcataatc atcgcccggc gtgatgcctg taatgttata
     8461 gcgccgatac tccgcacgca gcgggccgtt agcatcaaac accacacagg aagcgacggt
     8521 ttgttcgccc atggtatggc tgatgtcaaa gcactccatc cgcttcactt ccggcaattt
     8581 caacacgctg gcaagcgcgg tcagtcgctg gtgaacggta gattgctgcg aaagtttgct
     8641 ggttaaggcc gtcgccgcat tggtgcgcgc gagtttcaga taacgcgccc tatcgccgcg
     8701 aggtttggtt tgaacattaa tcttgcgtcc cgccagttct gaaagggaat cggcgagcag
     8761 cgttttatcg ctaagattaa aatcgagcag gatctcaccc ggtaaggtgc gcatctggct
     8821 gccttgtaaa tagaactgac ctacgaaggt ttctaccacc tcgctcagtt ccgtaccgcc
     8881 aggcactttc gggaaatagc tgcggctgcc gagcactttg ccctgacgaa tgaacaatac
     8941 gtggacacaa gccatgcccg catcgaacgc cacaccaata acgtcaaggt cgtcgccggt
     9001 attggaaaca aactgttttt cggtgacgcg tcgcaccgcc tgaatttggt cgcgaataca
     9061 tgccgcttct tcaaactcca gattctggct ggcagtttcc atacggctaa tgagttgcgt
     9121 aagcacctga tcatctttgc cagacaaaaa caggcgcaca tactcgacct gctgagcgta
     9181 ttcttcttca ctcaccagtc cttcaacgca cggtcccaga cagcgcccta tctggtattg
     9241 cagacacgga cgcgagcgat tgcgataaac actattttcg cactggcgaa tggggaaaat
     9301 cttttgcagt agcgccagtg tttcacgtac ggcatagcca ttcgggaacg ggccgaaata
     9361 ttcacctttg gcatgctttg caccacgatg catcgccaga cgcgggtggg tatcgccact
     9421 caggaagata aaaggataag atttatcatc gcgtagcaaa acgttgtaac gcggctgata
     9481 gagtttgatg tagttgtgtt ccagcaacag cgcttcggtt tctgtatgcg taaccgttac
     9541 atcaatttgc tggatctggg cgaccagcgc ttcggttttg cgcgaagcga ggttgctgcg
     9601 gaaatagctg gaaagccgtt ttttcaggtc tttcgctttg ccgacataga taaccgtacc
     9661 accagcatcg tacatgcgat aaacgcctgg ctggctggtt acggttttta aaaacgcttt
     9721 tgcgtcaaac tgatcactca ctgacttgat aatgtctccg cattacacag accatggcga
     9781 attgccaggt gagtcagctc aacatcgcca tgaatgttta gtttactgaa catacgatag
     9841 cggtagctgt tcaccgtttt cggactgaga ttgagctgtt ctgagatctc attgaccttc
     9901 tggcccttag tgatcatcag cataatctgc aattcacgtt cagacaaact ggcaaatggg
     9961 ctttctgttt tttctggttc gatctggctt aacgccattt gttgagcgat atcagaagca
    10021 atgtaacgct gccctgaata gacagaacga atcgcactca cgacttcctg cggagccgcg
    10081 cctttgctga ggtagccagc agcacctgcc tgcatgactt tcgctggtaa agggttttct
    10141 gtatggaccg taagcatgat gattttaaca tcagctgtgg aacgcgcgat tttacgcgtc
    10201 gcctcaagac cgccaatgcc cggcatactc atgtccatta gcaccacgtc aacggcattt
    10261 gtccggcacc acttaacggc gtcttcaccg cacgatgcct ctccgacgac ttttataccc
    10321 tttatatctt ccagaatgcg tcgtatccct gcgcgcacca gttcgtggtc atcaacaagt
    10381 agaacgttga tcaaaggaat atctccagaa atagggataa cgctactgat agttagtcat
    10441 tcgtatatta gcggttttta ttgcaacttt gaaacgttaa aaatggtggg tattcgattt
    10501 ttctctcgtt tttgtcaatt cgattgtcca cagttatggt cacgcccgtc accaacggct
    10561 taccggatcg tttcacagta agccctacga tgaaatgtga caaaaattga ctttattcag
    10621 caaaaatgaa aatcagcccg ctgaatatgt aacattaatt aacccgaaca atacgggcaa
    10681 aaaacattca tgataaaaat atttattggt cattatatta acgtatttta tagcactgcc
    10741 gatatcacgc tcaaaaaaca accactgcta tttttagcaa agcttatggt atactccgcc
    10801 gccttaacat ttttcaccgc aaattttcat tgcaacatga cgaggaaaat aaatgagtac
    10861 gcctgatttt tctactgccg agaataatca agaactggca aatgaagtct cctgcctgaa
    10921 agcgatgctg acgctgatgc tgcaggcgat gggacaagct gacgcgggcc gcgtgatgtt
    10981 aaagatggaa aaacagcttg cgctgatcga agacgaaacc caggctgcag tattttccaa
    11041 aacggttaag caaattaaac aagcctaccg tcagtaatat aaaaccggct gagagcgtgc
    11101 ctttcagccg gttattgaca aaatcatcgc tatctctgct gatgtcttcc cttccgccgt
    11161 ccatttcaga atttcttttt cgcgcttgct gaaattcatc tctggcgtca tcactatttc
    11221 atcatttaaa cgcatcagag ccatcagact ttcgcgcacc agtaactgca tttttaattg
    11281 cagttcatca ctaagaatgg gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc
    11341 cagcgcccga tttggcagca ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg
    11401 cgcggcttcc cataatggct gtgcttcgct gaataagtca tcattccaca ttaaatggtc
    11461 ctgactaaag ttttcagggt tcagcacagg atcaattgcg agaaaatttt ttgcctgata
    11521 ataactaacc cacgactcag ggtaattggt gtagaaagcc actttaggtc gcgtgaatag
    11581 taccgggtgg cgaacacata acgaatagta atcgtactcc agctgctgag cctgaagctc
    11641 tatttcatgg tagacctctt ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg
    11701 acgccagctg aaaaaatccg tatcctgcat agtaaaccgc aacgcccctg agagtgagta
    11761 tcatttataa tgataattca aactatcaca taaataagat ttatatataa tttatattat
    11821 tcaggcaatg aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc
    11881 tgatttgtta agcagtttta tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt
    11941 cggggctgat tttttcttat tattgcagca gaagcttctc gaggaactgg cgggtgcgag
    12001 gctgctgggg atcggcaaat aacgcttttg ctgccccctg ctcgactatc cgcccctggt
    12061 ccataaagat cgcccggtcc gcaacatccc gggcaaagct catttcgtgt gtcacaatca
    12121 ccatcgtgcg cttttcctgc gccagctggc ggatggtgtt caggacttcg cccaccagct
    12181 ctggatccag cgccgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagtg
    12241 cacgcgcaat cgcaacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt
    12301 ctttaccggc cagcccaact ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt
    12361 tcggttcgcc tttgacgatc accggccctt caataatgtt ttccagcacc gtacgatgcg
    12421 gaaacaaatt aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg
    12481 atttttgctg acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc
    12541 ccgcttccgg ttgttccagc agatttatgc tacgtagcaa tgtggttttg ccggaaccac
    12601 tcggaccgat aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca
    12661 ccgtctgacc gtggaatttt ttcaccaggt tcttaacttc aatggcactc attttggttc
    12721 tctctcctgg cgattaagtt gattctcaaa atggttctgc aacgtcgata acacagtcgc
    12781 catgatccag tagatgagcg aagccgccag gtacatggtg aacacctcca gcgtgcgcga
    12841 ggtaatcaac tgcgcctgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt
    12901 atcttttacc agactgataa agctgttcga cagcggcggc aacgccaccc gcgccgcctg
    12961 tggcaaaatc gcgcgacgca tggtctgcca cggcgtcata ccaatactgg cagcggcttc
    13021 ccactgacct ttatcaatag aagaaatggc ggcgcgcagc gtttcggcgg cataggcggc
    13081 agtgttcagc gacagaccaa tcattgctga cggaatggga tctaattcga taccaaactg
    13141 cggcaggccg taatagatca taaacagttg tgcgatgagc ggcgtaccac gaaaaataga
    13201 gatataaaaa cgcgccagcc agcgcacggg ccagattggc gacaggcgca tcagcgcgag
    13261 aataaacccg agtagtaagc caaaaaacat gccgccgata ctgagttgca gggtataccc
    13321 agcccctttc aacaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag
    13381 cgttcccgga gttaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga
    13441 ataaacagaa tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagcccc
    13501 gccatcgctt ttccggtata cacaggatca agcagaatac cttccagacg cgccagcaat
    13561 ttcactgctt ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc
    13621 cagagtaaaa tttccgctga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg
    13681 ttaaccactt tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg
    13741 ctttcaggca tcaggtgttc cagcccaaca gccagtccgg cgtgagttcc ggcactgccc
    13801 gatgcgacta ccaccgacga aatattaacc gccccttcac actgttgcgc gatttccagc
    13861 gcactctcca cataacctag cgcgccaaga gcattagaac cgccaaccgg aatgacatac
    13921 gggcgaaagc cttgtgcttc gactcgcgtc gccagctctt ccagttgggc attgggatcg
    13981 gtcagtgcgt cgcacatttc aatctgggta ttgaacagat ccagcaacaa acgattgccg
    14041 ttggttaaat agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc
    14101 agaccgagtt tcgccgcgac tgcggcagtc tggcgcacat ggttagactg gatcgcgccg
    14161 gcagtaatca gcgtatcggc accttcgcgc agggcatctg ccgcgagaaa ttccagctta
    14221 cgtaatttat tgccgcccat tgccatgggg gtgacctcat cccgtttgat gaaaatttcc
    14281 cgtccaagat aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac
    14341 tccaaccgtg gaaaacgggt taaattatgc agtggcataa cagcctccga tgcgtgttgt
    14401 tgtgattttc ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg
    14461 cccttttttg tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa
    14521 gagcttgcag agtgccgtct ttttgcattt ccgcaattgc atcattcacc gctttcagca
    14581 ggtcttcatt tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg
    14641 ttactgccag cgtatcgttg gttttcttca ccagatccag cgccgccaga cgatcaacga
    14701 ggatcgcatc gatacgccct acgcgcagat cctgatattt ggtcgggtca tcatcatagg
    14761 tacgcacatc gacaccctga acattctgcc gcagccactc ttcatagttg gtgcccagac
    14821 cgacacccac ttttttgcct ttcagatcag cagccgtttt aatggtgcct tcgttacctt
    14881 ttttcaccag cgcctgaata ccggaaatgg tgtaaggggt agagaaatcg tattttttct
    14941 tgcgctcgtc agaaatggtg acctgattaa tcaccacatc aatgcgttta gagtccagcg
    15001 acgccagcat accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg
    15061 ccagctgttg agcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac
    15121 tgaacggcgg ataagtgcct tccagcccta ccagcagcgt gccgcgctct ttgactttat
    15181 taagcagacc ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc gccacgccca
    15241 tcacacccat caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg
    15301 ttatgtctgt ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc
    15361 ttattcttat ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg
    15421 tacaggtttg gcgctgatag tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc
    15481 tggtggtggg cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga
    15541 gggaaatatt ttgctcatgt agatgattac ccaaacggcg cagacgaacg acatattcac
    15601 gcaccgtacc tgggctcatt tcagtttgct caaaaagaaa tttcttaaag ccgataatat
    15661 cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc
    15721 atgattgttg ttccgtttgc cagccttttt catcaagcag cgtgtccagg cgggaaatct
    15781 cgattttatt cactattttg ccgtcgcgaa ctaaggtaat gcgatcgagt aatttacggc
    15841 aatgcgcgca atgggtctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc
    15901 ttttcaggtg ctgcaccatc attaagaact cctggtagtc aaagttaaag tgcggcattt
    15961 actgacgtta taacttaccc agtttagtgc gtaaccgttt aatggcctgg ctgtgtaact
    16021 gactgacccg cgattccccg acctccagca ccgcgccaat ctctttgaga ttcagctctt
    16081 cctggtaata gagggttaat accagttttt cgcgctccgg caacgtttcg atggcttcca
    16141 tcacccgctg gcgcagatta ctgtccagca gttgttgtag cgggttttct cgctgatgat
    16201 catcagtaac cagttcgatg ctatcgccgt gctcttcacg ccactcatcg taggagaaga
    16261 gctggctgtt attggtgtcg agcagcattt gacgataatc ggcaatatcg atccctaaac
    16321 gttccgctac ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg
    16381 cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa
    16441 gttcatccag catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt
    16501 gtagggcgtc atagcgttcg acagcattaa gtaacccaat gccgcccgcc tgtagcagat
    16561 cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacacacca
    16621 gcgggacata acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg
    16681 aattcacgat aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag
    16741 ggtttttaat cggacgatta gtgggtgaaa tgaggggtta tttgggggtt acgcaaaaag
    16801 ccgaatgtaa aaaaccccgt cggtggcggg gtttgagagg cattcagacg attaaccctg
    16861 cagcagagac agaacctgct gcggtacctg gttggctttt gccagcacgg agttaccggc
    16921 ctgcaggata atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg
    16981 aatacgggac tgcgcttcag acaggttggt agtggtgttg ttcaggttgg tgactgcgga
    17041 atccaaacgg ttttgcaccg cacccaggga ggagcggaat ttgtcgatgg agctgatggc
    17101 ttcatccaga gctttcaggg gatcggctgt cgtagctgct tttgtttcag catctgtcgt
    17161 caatttacca tcagcatctt tataaactgc cttaccgctg ccgttagtca ctgaaccatc
    17221 atcctgtagg taaaaatcta cagtatcaga tgtggtcaac gcctcgttgt tatcaacata
    17281 aaccgaatca acaccttttg ttacagcgcc agttgtgccg ttgacgctat aacctacagt
    17341 agtgaaatca acattgtttc ccgaggttga tacagcttta tcgaaagctg cctgatcaac
    17401 ttttgcacct gttactgaat aagtaatagt gtccggcgta gtactattac cggtaaagga
    17461 gatagaggta ccgccaatgt caatggttgt cgctttggta ttattggctg caagtgtgga
    17521 cagactagcc tcagtcaaag aagcaccaga gccgtttttc gttaagcgcc cagttgtatc
    17581 aatgtagagt ttatctccat tgcttgccgt aattttacca tcactgctga tctgaacatc
    17641 ctgtgcagaa ccatcgatag taatagtgcc cttagcggta tcaccaactc ccggggtcaa
    17701 atatttctga acatcggcag ctgaagccgt tgtggtatca aaagagtatg acttacttgc
    17761 actgtcatac ttgtaattcg ttgcactagc cgccgttccg aagccgttat taatggttgc
    17821 ctgaacagta tcaccatcac taactccagc cagcaaatca gacgctttag cagcatcgta
    17881 acccgcactc acagtatatt tgttgccgac cactgttgca ttagcagcta ccaagtcagc
    17941 tttagatgca gcagtattag ccacagcccc gccaccattc acattaaacc cattcagccc
    18001 cagcgtatca gagtcaattt tcttcagatc aatagtgata gtctggccgt cattcgcacc
    18061 aacctgaatt ttcatcgaac cgtcttttgc cagtacgttc acgccgttga actgggtctg
    18121 accggatacg cggtcaattt cgtcgagacg ggatttgatt tcgtcctgaa tggagtccag
    18181 atccgaatca gagttagtcc cggtagaagc ctgaaccgtc agctcacgga tacgctgtaa
    18241 gttgttgttg atttcggaca gcgcgccttc agtggtctgt gcaacagaaa taccgtcgtt
    18301 ggcgttacgt gcagcctgag tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc
    18361 ctgacccgcg gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat
    18421 agaactcgac agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct
    18481 gttggtatta atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt
    18541 tttgggtttc cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca
    18601 acctttagaa tttttttcac aaacagccat tttttgttag tcgacgaaat actcttttct
    18661 ctgcccctta ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc
    18721 cgataacccc ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat
    18781 ttcatcgctg ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc
    18841 cgcgcaaaaa gtgacgctaa cccctatttc aaatcagcaa tcgtcgttta ccgctaaact
    18901 tagcgcctac ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt
    18961 gtctaaagcc gatctttttt ccgccaccag caccaccagc agcaccaccg cgttcagtgc
    19021 caccaccgcg ggtaacgcca tcgccgggaa atacaccatc agcgtcaccc atctggcgca
    19081 ggcgcaaacc ctgaccacgc gcaccaccag agatgatacg aaaacggcga tcgccaccag
    19141 cgacagcaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
    19201 cgcggctaac tcatcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt
    19261 aagtgcgagc atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga
    19321 caccggcctt gataatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt
    19381 tatgggctat gacgccagtg ccagcagcaa cggtatggag gtctcggtcg ccgcccagaa
    19441 tgcgcagctg acggtcaaca acgtcgccat cgagaacagc agcaacacta tcagcgacgc
    19501 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat
    19561 cactcaggac acctccaaag cgcaaacggc gattaaagac tgggtgaatg cctataactc
    19621 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
    19681 ccagagttct agcaatggcg cactgctcgg cgactccacg ctgcggacga ttcagacgca
    19741 gttgaagtcg atgctgagta ataccgtcag ttcttccagc tataaaacgc tggcgcagat
    19801 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
    19861 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
    19921 cggcatcaca accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
    19981 taaagccgct accgatggcg ttagtaaaac cctgaataag ttaactaaag actacaacgc
    20041 cgccagcgat cgcattgatg cgcaggtcgc gcgctacaaa gaacaattta cccaactgga
    20101 cgttttaatg acctcgttaa acagcaccag cagctactta acgcagcagt tcgaaaacaa
    20161 cagtaattcc aagtaatcat tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
    20221 ccaggcctat gcgcaaattg gcgtcgaaag caccgtaatg agcgccagcc aacagcagtt
    20281 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgac tgtttatgca
    20341 ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
    20401 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttaattgc
    20461 tctttatagc tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc
    20521 agtcgaagag gtggaagcat taatgcgtaa tattgccgat gcctggaaag agtctttact
    20581 ctccccttct tcgattcagg acccagtctg atgaacaatg caccgcattt gtatttcgcc
    20641 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
    20701 gacgaactca tcgctagcga aatggcgtat gtgaatgcgg tgcaggagat cgcacacctg
    20761 actgaagagg ttgccccgtc cactacgatg caggagcagc tccgcccgat gctgcacctg
    20821 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg
    20881 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc
    20941 ggctttgtgc tggctccgca ggataacctc ttttgaatct taatgagccg atggctcgcg
    21001 aataatccga ttacggctac gcttctaata ttccccttga atggagtcga agaatgcgta
    21061 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
    21121 aactggccga gcgcgcagac ggttttaatg atattggtat caatatggtc tggttgccgc
    21181 ccgcctataa aggcgcatcg ggcggctatt cggtcggcta cgactcctat gatttatttg
    21241 atctaggtga gtttgatcag aaaggcagca tccctactaa atatggtgat aaagcacaac
    21301 tgctggccgc cattgacgcc ctgaaacgta atgacattgc ggtgctgctg gacgtggtag
    21361 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg
    21421 atgaccgtac gcaaattgac gaagaaatca ttgagtgtga aggctggacg cgttacacct
    21481 tccccgctcg tgccgggcaa tactcgcagt ttatctggga tttcaaatgt tttagcggta
    21541 tcgaccatat cgaacaccct gatgaagatt gcatttttaa aattgttaac gactataccg
    21601 gagaaggctg gaacgatcag gttgatgatg aactcggtaa tttcgattat ctgatgggtg
    21661 agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
    21721 tgatggaaca aacgcaatgc gacggctttc gtcttgatgc ggtcaaacat attccggcat
    21781 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
    21841 tggctgaata ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag
    21901 gcaaaaccat gctgtttgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
    21961 gtcgcgacta cgacatgacg cagatcttca ctgggacgct ggtggaagcc gatccttttc
    22021 acgccgtaac cctcgttgcc aaccacgaca cccaaccgtt gcaagccctc gaagcaccgg
    22081 tcgaaccgtg gtttaaaccg ctggcgtatg ccttaatttt gttgcgggaa aatggcgttc
    22141 cttcggtgtt ctatccggac ctctacggtg cacattacga agatgtcggt ggtgacgggc
    22201 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
    22261 agcgtttcgc ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta
    22321 gccgcagtgg caccgacgaa tatcccggct gcgtggtggt catgtcgaac ggagatgatg
    22381 gcgaaaaaac cattcatctg ggagagaatt acggcaataa aacctggcgt gattttctgg
    22441 ggaaccggca agagagcgta gtgacagacg aaaacggcga agcgaccttc ttttgcaacg
    22501 gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta aatccatccc cggcggcaag
    22561 ccggggagat ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca
    22621 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
    22681 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatcagac
    22741 atcaatgtca acttacccgg cacggcaatc ccccgctgcc actgacggca atccaaggta
    22801 tcgccctctt tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc
    22861 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
    22921 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
    22981 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gctctgatgt
    23041 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
    23101 ccccgaaaat aaatttgcat ggtgtatgat tcgcggctct tgtttcaaac cttctgagtt
    23161 cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg
    23221 cacccatccc tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg
    23281 gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggtcagctc ctgcagctgt
    23341 ttggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat
    23401 taacccgcat cgacggcatg atgatcctcg gtatgtttgg cggctgcttt gctgctgcgc
    23461 tgtgggccaa caatgtcaaa ctacgcatgc cacgcagccg tatccgcatt atgcaggcca
    23521 tcattggcgg cattatcgcc ggttttggcg cgcgtctggc aatgggctgt aacctggcgg
    23581 cgttctttac cggcattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg
    23641 ccattggttc atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga
    23701 aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc
    23761 gttttcgtct cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga
    23821 tgaaccaacc aaaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg
    23881 aacgtgcgca aatctgcttt acttcggcgt tccgcgatat gtggatcacc ggacgtaccc
    23941 atatggcgaa agcaatcatt atcggtatgg cggtgagtgc catcgggatc ttcagttacg
    24001 tacagttagg cgttgaacct aaaatcatgt gggcgggacc aaatgcggta attggtggtt
    24061 tactttttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg
    24121 cggtagaagg ccaggtgcac tactggtggg tcggtctggg caacgtgatc ggctcaacga
    24181 ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca
    24241 acctgctgaa aacttttggt ccgatgggag gcctgctggt gacatatttg ctgctgtttg
    24301 ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga
    24361 ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga
    24421 accctgccct tatccggcag tcgcaaccct tgaggcgatg ccgcaattaa aaaaaggtga
    24481 gatcctagaa gtggtgagcg actgtccgca gtcgatcaat aatattccac tggatgcgcg
    24541 taatcacggc tacactgtgc tggatattca gcaagacggg ccgaccattc gttatttaat
    24601 tcagaagtaa tccctcattc ccgctgtggc ccacagcggg aacatcgtta cctctgacac
    24661 atagatttta cccgccccct ctcctattct tttcagtatg atctcaggag gttgctatgt
    24721 gtggacgctg tgcccaatcc caaacgcgtg aggattacct tgcgcttctc gcggaagata
    24781 ttgaacgcga tattccctac gatcccgaac ccattggcag atacaatgtc gcgccgggaa
    24841 ccaaagttct tctgctcagt gaacgtgatg aacaccttca tctggatccg gttttctggg
    24901 gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta gaaactgcct
    24961 caaccagtcg catgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg
    25021 gctggtttga gtggaaaaaa gaaggcgaca aaaaaagcct tattttatct atcgcgctga
    25081 cggacaacct gtttttatgg ccgcgatagg tagcataccg tttgagcgtg gtgacgaagc
    25141 cgaaggattt ttgatcgtca ctgcggcggc agatcaaggt ctggtagata ttcatgaccg
    25201 ccgcccgctg gttctgtcgc cagaagctgc acgggaatgg atgcggcaag acattggtgg
    25261 taaagaagcc tcagaaatcg ctaccagaag ctgtgtgcca gcaaaccagt tcacctggca
    25321 ccctgtgtcg cgcacggtgg gtaatgtcaa aaaccagggc gcggagttaa ttcaacctgt
    25381 ttgttgacat cctccacgcc ctgaatgacg aggccccctg ctacgttca
//
LOCUS       DADLTF010000048        25077 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.048, whole genome shotgun sequence.
ACCESSION   DADLTF010000048 DADLTF010000000
VERSION     DADLTF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.048"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..614)
                     /locus_tag="JN749_001862"
     CDS             complement(<1..614)
                     /locus_tag="JN749_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAY7368301.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
     gene            complement(611..1873)
                     /locus_tag="JN749_001863"
                     /pseudo
     CDS             complement(611..1873)
                     /locus_tag="JN749_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            complement(1870..2778)
                     /locus_tag="JN749_001864"
     CDS             complement(1870..2778)
                     /locus_tag="JN749_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAY7368302.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2945..3742
                     /locus_tag="JN749_001865"
     CDS             2945..3742
                     /locus_tag="JN749_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7368303.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRINVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GSCIYLDAGTTTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3793..4445)
                     /gene="pphB"
                     /locus_tag="JN749_001866"
                     /pseudo
     CDS             complement(3793..4445)
                     /gene="pphB"
                     /locus_tag="JN749_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4551..7112)
                     /gene="mutS"
                     /locus_tag="JN749_001867"
     CDS             complement(4551..7112)
                     /gene="mutS"
                     /locus_tag="JN749_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAY7368304.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLLRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7188..7367
                     /locus_tag="JN749_001868"
     CDS             7188..7367
                     /locus_tag="JN749_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368305.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7399..7752
                     /locus_tag="JN749_001869"
     CDS             7399..7752
                     /locus_tag="JN749_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HAY7368306.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7789..9867)
                     /gene="flhA"
                     /locus_tag="JN749_001870"
     CDS             complement(7789..9867)
                     /gene="flhA"
                     /locus_tag="JN749_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HAY7368307.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9941..10951)
                     /gene="hypE"
                     /locus_tag="JN749_001871"
     CDS             complement(9941..10951)
                     /gene="hypE"
                     /locus_tag="JN749_001871"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HAY7368308.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10948..12069)
                     /gene="hypD"
                     /locus_tag="JN749_001872"
     CDS             complement(10948..12069)
                     /gene="hypD"
                     /locus_tag="JN749_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HAY7368309.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12069..12341)
                     /gene="hypC"
                     /locus_tag="JN749_001873"
     CDS             complement(12069..12341)
                     /gene="hypC"
                     /locus_tag="JN749_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HAY7368310.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12332..13204)
                     /gene="hypB"
                     /locus_tag="JN749_001874"
     CDS             complement(12332..13204)
                     /gene="hypB"
                     /locus_tag="JN749_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HAY7368311.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13208..13558)
                     /gene="hypA"
                     /locus_tag="JN749_001875"
     CDS             complement(13208..13558)
                     /gene="hypA"
                     /locus_tag="JN749_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HAY7368312.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13770..14231
                     /gene="hycA"
                     /locus_tag="JN749_001876"
     CDS             13770..14231
                     /gene="hycA"
                     /locus_tag="JN749_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HAY7368313.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14356..14967
                     /locus_tag="JN749_001877"
     CDS             14356..14967
                     /locus_tag="JN749_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7368314.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPACVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14964..16822
                     /gene="hycC"
                     /locus_tag="JN749_001878"
                     /pseudo
     CDS             14964..16822
                     /gene="hycC"
                     /locus_tag="JN749_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
     gene            16825..17721
                     /gene="hycD"
                     /locus_tag="JN749_001879"
     CDS             16825..17721
                     /gene="hycD"
                     /locus_tag="JN749_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HAY7368315.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            17739..19424
                     /locus_tag="JN749_001880"
     CDS             17739..19424
                     /locus_tag="JN749_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HAY7368316.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTAFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            19434..19976
                     /gene="hycF"
                     /locus_tag="JN749_001881"
     CDS             19434..19976
                     /gene="hycF"
                     /locus_tag="JN749_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HAY7368317.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KENFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            19976..20743
                     /gene="hycG"
                     /locus_tag="JN749_001882"
     CDS             19976..20743
                     /gene="hycG"
                     /locus_tag="JN749_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HAY7368318.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            20740..21150
                     /locus_tag="JN749_001883"
     CDS             20740..21150
                     /locus_tag="JN749_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HAY7368319.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            21143..21613
                     /gene="hycI"
                     /locus_tag="JN749_001884"
     CDS             21143..21613
                     /gene="hycI"
                     /locus_tag="JN749_001884"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HAY7368320.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            21683..22615
                     /locus_tag="JN749_001885"
     CDS             21683..22615
                     /locus_tag="JN749_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368321.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEGGTRTDFLQSGYEDFGGNYIAHKCLHIVNL
                     DLMSYSFRTHKDRTRRCGCLLTIRKLKLKNAHHF"
     gene            22599..22910
                     /locus_tag="JN749_001886"
     CDS             22599..22910
                     /locus_tag="JN749_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HAY7368322.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            22907..23326
                     /locus_tag="JN749_001887"
     CDS             22907..23326
                     /locus_tag="JN749_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7368323.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(23441..24865)
                     /locus_tag="JN749_001888"
     CDS             complement(23441..24865)
                     /locus_tag="JN749_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7368324.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAIGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24874..>25077)
                     /locus_tag="JN749_001889"
     CDS             complement(24874..>25077)
                     /locus_tag="JN749_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7368325.1"
                     /translation="PGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFILTLLLGFEDI
                     PVEEAAAQARKHQPTVAKEVSLN"
BASE COUNT         5697 a         6658 c         6747 g         5975 t
ORIGIN      
        1 cctgcggtca gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc
       61 tccagctctt ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca
      121 tgatttgcca gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg
      181 cgtttatcgc ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg
      241 tatggtgtga acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc
      301 gtcgaccatg tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc
      361 gccagatgaa agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg
      421 gcgactttgg aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc
      481 agtaaattcc cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatagccg
      541 cgctgaaaga atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt
      601 gcgaaatcgc tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga
      661 agttgccaga tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc
      721 cgggggaaat ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc
      781 cggaggtctc accacctgcg acaataaagc gtgtcatgcc ttccgctgct aaccgcgccg
      841 ctagttgaga aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt
      901 gaattgctgc caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt
      961 cctggcccag aacccactct gccagttcgt gagcataagc ggccagagtt tcggttgaga
     1021 ggcagcgtgc cacatcaact tcacgggctg gtgcaatttg acggtaatgt gccacctagc
     1081 ggttggtcat ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc
     1141 ctgcttcgcg agcctggtta ccgttttctt gcgcccactg ccgggccagg ccaatcgcca
     1201 gaccagaacc gcccgttacc agtggggcat cgcgcaaggc ttctccctga atttccagat
     1261 ggtgttcggt cagcgcatca agcaccgcgt agcggtagcc ctcttgctgt aagcgagcca
     1321 gctcttgacg aacggcatcc acaccttgtt cgaaaacatg tgccgaaacg acgccgcagc
     1381 gccctgtgga ttgcgcttca accagacggg gaagatagct gttggtcatg ggatttaccg
     1441 ggtgatggcg catcccggat tcggccagca gttgattcat tacgaacaaa tacccctgat
     1501 aaaccgtacg tccgttgacc ggcagggccg gagagaagac cgtaaacggc gtgtcgagag
     1561 catccattaa tgcatcggta accgggccaa tattaccttt cgccgtactg tcgaaagtag
     1621 agctgtattt gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca
     1681 gcgattgctg tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg
     1741 cgtcgattgc ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac
     1801 cgttttccac cagaaaactg gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc
     1861 caatcttgat catgatttcg ctcccggtag agtgatgcca gagaaaatct tgataactgc
     1921 gctatcgtct tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt
     1981 tgaggccaat ggcagcggga agtgcagggc tttggctgta tcggcaacca gaccaagatc
     2041 cttaacaaaa atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat
     2101 ccggttttcg aacatccagg aatttccggc ggcattggtc acgacgtcat acatcacatc
     2161 cagcgggatc cccgcacggg ctgcaagtgc catcgcttcg gctccggcag caatatgtac
     2221 gcccgctaac aactggtgaa taatttttac ggtcgaacct agtcccggtt ctgcacctat
     2281 gcgataaact tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc
     2341 gctaccggag gccatgacag tcatttcacc gttagcggct tttactgcac caccagaaac
     2401 tggcgcatcc agcatttcca gatcgaatcc agccagagcg gtagcaattt cttgcgcatc
     2461 agcactagcg atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac
     2521 gcctgtttca ccaaacagca cctgtttaac ctgggccgca ttgaccacca gcaccagcag
     2581 agcgtccagt ttttcggcaa acgtcgcggc gttatcagaa accccgcaag cacctgcctc
     2641 tttcaacgta gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa aacctgcgcg
     2701 gacacatgac agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa
     2761 ctcagatccc gttttcatct gctctccttg ttaatttaag tgatattttg tttgacattg
     2821 tgaatataag cgctgggaga taacgatatg gtgagctgat tcacataaat taacattttt
     2881 gtgttatttt atgtgaacta agcgttagtt gcgccgcgca cgtttcgcag gcaaatagcg
     2941 tagaatgtca gcaggacaaa ggaaggagca aaagttgata cccgtagagc gtcgccaaat
     3001 catccttgag atggtagctg aaaaaggcat tgtcagtatt gctgaactaa cggacagaat
     3061 taatgtgtca catatgacca ttcgtcggga tttacaaaaa ctggagcagc agggagccgt
     3121 tgtgctggtg tccggaggcg tccagtctcc gggacgcgtg gcgcatgaac cttctcatca
     3181 ggtaaaaact gcgctggcaa tgacgcaaaa agcggctatt ggcaagctgg cggcaagtct
     3241 tgttcagccg ggaagttgta tctatctgga tgcgggaacg accacgttag cgatagcaca
     3301 gcatctgatt cacatggagt cactgactgt ggtcacaaac gatttcgtta ttgcgaacta
     3361 cttgctcgac aacagtaatt gcacaattat tcacactggc ggtgcagtgt gtcgggaaaa
     3421 ccgttcctgt gtcggggaag ccgctgcgac catgctgcgc agcctgatga ttgatcaggc
     3481 ttttatttct gcatcgtcat ggagtgtgcg ggggatttct acgccagcgg aagataaagt
     3541 cacggtgaaa cgggcgattg ccagtgctag ccgccagcga gttttggtct gtgatgcgac
     3601 gaaatatggt caggtggcga catggctggc gttaccatta agcgagtttg atcagattat
     3661 tacagacgac ggtttgccgg agagtgccag tcgcgcgctg gcgaagcagg atctctcttt
     3721 gctggtagcg aaaaatgaat aatggcctgc aataacatct ggttactcat gcttcacaga
     3781 agaagcatga gactacctta ttttataaaa tgacagccgc ccacttttcg gcgatccggt
     3841 atcaatataa atctggttag cgaacgtctg aatgttatca aacatcatat gtccaaatat
     3901 aaaataatca gcgccgttta tttgttgtaa ctcgccatta agcgatttct gcacacgata
     3961 aacaggccag agtaattcgc tctccgctat ttctttgtca aagagatatt catcacccgg
     4021 ataatctgca tgtgcgatga catattttat tatgtcgtta gtgatttcaa taatatgtgg
     4081 aaggtgatgg aatttcagca acagatctat tgcctcttgt tgcaatcatt taaatcgaaa
     4141 aaccagtcac caccgctggc aagccacata ttgccatcgc cggtcgcgaa cgcatccagc
     4201 gccattgctt cgtggttgcc tttaacggag ataaaccagg gctggtttag caggcgcagg
     4261 acgtcaagac tctccggccc acgatcaata ttatcgccga cagaaataag taagtcggtt
     4321 tcggggaaaa aagagagttg atgtaagcgg gattgtaata actgatattc accatgaata
     4381 tcaccaacga cccatatatg gcgatagtga tgggcattga ttttttgata gcgtgtagat
     4441 ggcatggttt taccctgtaa aataagcttt cctattatac agggtatttt tatttgattc
     4501 gtcagttgtc gttaatattc ccgatagcaa aagactatcg ggaattgtta ttacaccaga
     4561 ctcttcaagc gataaatcca ctccagcgcc tgacgcggag tcagtgaatc cgggtcgaga
     4621 ttttccagtg cttcgaccgc aggcgaagtt tcttctggca ctgacagcaa agacatctgc
     4681 gtaccatcca cttgcgtcgc ggcggcgttc ggcgaaatgc tttccagctc acgcagtttt
     4741 tgccgtgcgc gcttaataac ctctttcggt acgcccgcca gagcagcaac cgccagaccg
     4801 tagcttttgc tcgccgcgcc gtcctgcacg ctgtgcataa aggcgatagt gtcgccgtgc
     4861 tccagcgcat cgagatgtac gttggcgacg ccttccattt tctccggtag ctgggtcagc
     4921 tcgaaatagt gggtggcaaa cagcgtcaat gccttaatct tattcgccag attttctgcg
     4981 cacgcccacg ccagcgacag accatcgtag gtggacgttc cacgcccgat ctcatccatt
     5041 aacaccagac tgtattcggt ggcgttatgt aaaatattgg cggtttcagt catctccacc
     5101 ataaaggttg agcgcccgga agccaggtca tctgccgcgc ctacgcgggt aaagatgcga
     5161 tcgataggtc caatctcgac tttttgtgcc ggtacatagc tgccgatgta ggccatcagc
     5221 gcaatcagtg cggtctggcg catataggta cttttaccgc ccatgttcgg gccggtaata
     5281 atcaacatac ggcgctgcgg cgacagattc agcgggttag cgataaatgg ctcattcagt
     5341 acttgttcaa ctaccggatg gcgaccttcg gtaatgcgaa tgcccggttt atcaatgaag
     5401 gtcgggcagg tgtagttcag ggtataggcc cgttccgcca ggttaaccag cacgtcgagt
     5461 tccgccagcg cgctcgcgct ctgttgcaac gcttccagat gcggcaacag caggtcgaac
     5521 agctcttcat aaagctgttt ttccagtgcc agtgctttgc cttttgaggt gagaacttta
     5581 tcttcgtact cttttagctc tggaatgatg tagcgctcgg cgtttttcag cgtctggcga
     5641 cgcatgtagt tgatgggtgc cagatggctt tgcccacggc tgatttgaat gtagtagccg
     5701 tgcaccgcat taaagccaac ttttagcgtg tccaggccgg tacgttcacg ctcgcggact
     5761 tccagacgct ccagataatc ggtcgcgccg tcagccagcg cgcgccactc atccagctct
     5821 tcgttatagc ccgatgcgat aacaccaccg tcgcgtaaca gcaccggcgg tgtgtcgatg
     5881 attgctcgct ccagcagatc gcgcagctcg gcaaactctc ccatcttctc acgtagcgcc
     5941 tgtaccggtg cactatcgac agtttctaac tgcgcacgca gctccggcag ttgctggaaa
     6001 gcgtggcgca tacgggccag atcgcgtggg cgagcagttc gtaaagccag acgtgccaga
     6061 atacgttcca ggtcgccgac ctgacgcagt accggctgca actcggcggt gaaatcctgc
     6121 aatgcgccaa tagtttgctg gcgctcaagc aacacgcgag tatcgcgcac tggcatatgc
     6181 agccagcgtt tcagcatacg actacccatc ggcgtgacgg tgcagtcgag cacagaagcc
     6241 agcgtatttt ccgcaccgcc cgccaggttc tgagtaattt ccaggttacg acgtgtcgcg
     6301 gcatccataa tgatgctgtc ctgctcacgt tccatggtga tagaacgaat atgcggcagg
     6361 gtcgtgcgtt gggtatcttt cgcatactgc aacagacaac cggcagcaca aagtccgcgc
     6421 ggcgcgttct cgacgccaaa accgaccaga tcgcgggtgc caaattgcag attcaactgc
     6481 tggcgcgcgg tgtcgatttc aaactcccac agcgggcgac ggcgcaggcc gcgacggcct
     6541 tcaatcagcg acatctcggc gaaatcttct gcatacagca gttccgccgg attagtgcgt
     6601 tgcagctctg ccgccatcgt ttcgcggtcg gccggttcgc tcaggcgaaa acgcccggag
     6661 ctgatatcca gcgtcgcgta gccgaaacct ttgctgtcct gccagatagc cgccagcagg
     6721 ttgtcctgac gctcctgtaa cagggcttca tcgctgatgg tgcctggcgt aacgatacgc
     6781 acaactttgc gctcaaccgg acctttgctg gtcgccggat cgccaatttg ttcgcagatg
     6841 gcaacggact ctccctgatt caccagtttg gcgagatagt tttccaccgc atggtaggga
     6901 atccccgcca tcgggatcgg ctctcccgcc gaagcaccgc gtttggtcag tgaaatatcc
     6961 agcagttgcg acgcgcgttt tgcgtcgtca taaaacagtt cataaaaatc acccatccgg
     7021 taaaacagca ggatctcggg atgctgggct ttcagcttga gatactgctg catcatgggc
     7081 gtatgggcgt cgaaattttc tattgcactc atggggttat gtccggttcc ctgatattaa
     7141 atggggtgtg atggttttta ttttcgttgt aatcacataa ggcgcacatg ataccggagt
     7201 taatcatttc aggggaagaa gcgaacggcg agttcttgat gctggttccg ggaaagatgc
     7261 tgttttgcag cttttgcgtg atgcttatga agcaagtacg caaaattgta tcgattggcg
     7321 aacccttctt ctttagagtc atagtgccct ttaagattaa gatgtaatat aaatgcatct
     7381 taatgaggtg gaggcagcat gtccggcaag cgtatctctc gtgaaaaact gacgattaaa
     7441 aaaatgatcg atctttatca agcgaaatgc ccgcaggcgt cagcggagcc ggagcattac
     7501 gaggcgttgt ttgtttacgc gcaaaagcgg ctggataaat gtgtgttcgg cgaagagaaa
     7561 ccagcctgta agcagtgtcc ggtgcactgt taccagcctg caaagcgtga agagatgaaa
     7621 cagattatgc gttgggccgg gccgagaatg ctgtggcgtc atccgatctt aaccgtgcgt
     7681 catctcatcg atgacaagcg tccggtgcca gaactgccgg aaaaatatcg tccgaaaaaa
     7741 cctcgtgaat agtatgccgg acaggcagat ctgtccggca atttgcagtt aaatcaatgc
     7801 cgatttatca attcccagcc gcttcatccg tgacagcagg gtcgtgcgtt tcagccccag
     7861 acgttgcgca gcgcctttag gcccggcaac cacgctgttg gtttctttca gcacgcgcac
     7921 aatcaactga tattcatctt cgccctcctg ggcgacaacc gttgcggcag gcggcgtttc
     7981 aggctccggt aaagcaatat ctggcaatga cagctgcagc acgttgccgc gtgttagcaa
     8041 tactgcgcgc tcaatgacgt tttccagttc gcgtacgtta cccggccact ccatgttgct
     8101 caaggtgcgc agcgtctcgg caggaatgct gtcgatattg cgccccagac gacgggcaat
     8161 tttgaaggta aaggctttcg ccagcagcgg aatatcttcc ggacgctcgc gtagtggcgg
     8221 caggtgaatc gggaatacgt tcaggcggta atagagatcg ctacggaact ctcggtcggc
     8281 gaccattttt ttcagatcgc ggttagtcgc ggcaattaaa cgtacgtccg tctgaatgat
     8341 tttgttgctg ccgagacgtt caaactcctg ttcctgcaat acacgcagca acttcggctg
     8401 taactccagt ggcatatcgc ccacttcgtc gaggaacaga gagcttttat ccgccagttc
     8461 aaaacgacca atgcgctggg cgctggcacc ggtaaaagcc ccacgctcat gaccaaacag
     8521 atcgctttcc agcaatccgg caggcatcgc cgcgcagttc attttgacca tgcggcgatt
     8581 attacgccca ctgagattat ggatcgcacg ggcaatcagc tctttacccg tgccagtttc
     8641 accgaggatc agcacggtac tgtcactttg cgccaccatt tcaacttgtt taagcacgct
     8701 gtacatggct tcgctgcggc caataatctc gccaaattca ctatcaacat tgttgagttg
     8761 ctcggtcagg gcgaggtttt catcaaccag ccgttctttc agacgatgga tttcctgata
     8821 ggcgagggcg ttatcgacag cgattgccac acgttcggca atctggcgca gcaaattcag
     8881 attggtagtg gtaaacactt tctcttcgca ttgcgccagt ttcagcacgc ccagcatggt
     8941 gtcgccagac atcagcggta acaggcacaa ggtttgaatc tggttgcccc aggtgtcgaa
     9001 caacatgcgt tcataggggg ctaaatcgtc ccgctcgtag agattgatca gcagcatctc
     9061 tttacttttg aacacgcgtt cggtgagggt tccggcttca tcgacttcgc tctgttcgtg
     9121 ggcgggatgt tgtttatcaa gatagtgagt ggagtagatg ttgagtttgt ttttacggtg
     9181 gctgcgtaag acaatgctga tatcgtcaat gtcgaaatag taatggattt ctttggcgac
     9241 ttcgctgacc agttcgtcca tatccaggcg ggaaagcacc gcgttggtaa tggcaaccag
     9301 gatgcggaag ttatcgcgct cccggcataa caactcatag tcgacattgt tgacgacgcg
     9361 gctctggatt tgttcggtga cgacagaaac gatctgcgta aatgtttgca gacgattgaa
     9421 ctctttttcg ctccagggac gatcgtcata acgaataaat tcacagccac caaaaatatg
     9481 ccctcccgcc gccagtggca tcaggcaata gtgaccaaat ttggggtata gcccacctgc
     9541 ggccagctgc ggccaggttt cacaaaattc ttcgtaactg caatgcagcg tatcagggcg
     9601 cgacaaaatg ctgcgtaccg gaccgtgtgc cagaacagtt tcgtcttcat atttaatggg
     9661 ggtgtctttt tcacgcgacg cgtaataaga cgcacgttga gtctgcgctt gccacaacac
     9721 aatagccgcg ttgtcggcga gcgcagaacg ctttaccagt tgcgaaagag cctcacacag
     9781 cgaggccaga tcgggctgct gcaatagtgt ccgagtgatg tcgaacaacc cttgctgtcc
     9841 gagatcactc atcggtgtat atgacatttt gttcttccag gaaagcaccg caaagcggtg
     9901 caaaaggtca ttgactaact ataggagatt tagaatttta ttagcatata cgcggaagcg
     9961 gttcggcgtg tggtaaatcg agggttcgtt tcacgccata cagaccggca agacgaacac
    10021 ctttacgttc caccacttca ccaatcagcg ccgcgtcttt ccccagtgga tgggaatgta
    10081 acgctgccag cacttgctct gccgcgttgc gttcaacggc gatcaccagt ttaccttcgt
    10141 tggcaaagtt cagggcgtcc agtcccagca attcgcaaac gccgcgcacg gcaggtttaa
    10201 ccggcagcgc tgattcagaa atttcaatac cacaaccgca ggctgccgcg aactcatgaa
    10261 ccaccgcgtt tacaccacca cgggtggcat cacgcagcgc tttcacgccg ggaatgtcac
    10321 gcagcgtctg aataagcggc gtcagcaccg cgcagtcgct gaccagttcg ccatccagcc
    10381 ccagctgctc acgcaggtta aggatagtcg ccccgtggtc gccgagtgta ccgctaacca
    10441 gcaatacatc gcctgcggtt agcgtctgtg cgccccagtg aatattcgcc ggaattgcgc
    10501 ccatgccagc ggtgttgata aacagtttat ctgccgcgcc gcgctgcacc actttagtat
    10561 cgccagtaac gatggcaatg cctgccgtgc gggcggtttc tgccatgctg gtcactgcgg
    10621 ctttcagtgt ctccatcggc aatccttctt cgaggataaa gccacaggag agatagcgcg
    10681 gaatagcgcc actgaccgca acgtcattcg cggtgccgca aatcgccagc ttgccgatat
    10741 taccgccagg aaagaacagc gggtcaatga cgtaactgtc ggtggagaac gccagacggt
    10801 cgccttccgc taccagctgc gccagatcaa gacgtgcctg atcttcctgc tctgccagcc
    10861 acgggttggc aaaggcttcc ataaacaggc tgttgattaa ttgctgcatc gcctggccgc
    10921 cgctgccgtg ggcgagttgg atattattca cgcttcactc tcctgctgac gatactgata
    10981 ccacgcggcg cacgctcctt cggaggagac catcagtgca ccaaatgcgg tttgaggatt
    11041 acaggtgtta ccaaacagcg ggcattgatg cggcttacat ttgcccgtta atacctcacc
    11101 acaacgcgcg cgcgggtcat cgcagacctg ctgcggtgcc gggcggaaat gtgcttcggc
    11161 atcgaatcgt tgataatccg gcgtcaggtg cacgccagaa gattcaatca cgcctaagcc
    11221 gcgccattcg ctgtcgccgt tgacacagaa cacatcggca atcgcctgtt gcgccagcag
    11281 gttaccggca tccggtacca cccgacgata ctgattctct accttgctgt gggccgctat
    11341 tttctgctcc accagcatga ccacgccttg cagtagatca aggggttcga aaccagcgac
    11401 caccagcgga cgatgaaaat cgctggcgat aaaattatag gcatcagtgc cgataaccat
    11461 actaacgtgg cctggcgcga ggaacgcgtc gataccgtta tccggctgtt ccagcaaact
    11521 gcgcagcgtc gggataagcg taatgtgctg gcagaagaag taaaaattct gcacatcacg
    11581 cgctttcgcc tgttgcagag tgatggcggt ggtcggcatg gtggtttcaa aacctaagcc
    11641 gaagaacacc actttgcggg ttggattctc ctgcgccagt ttcaacgcat ccatcggcga
    11701 gtaaacgatg cgcacatcgg caccgcgtgc ttttgcctgc aacagcgatc cctgtttccc
    11761 tggtacgcgc atggcgtcgc caaaggtaca gaagatgact tccggatggc tggcaatctc
    11821 cacgcaggtg tcgattctac ccatcggcag tacgcacacc gggcaacccg gaccgtggat
    11881 aaactcaacg ttttcaggca gtaactggtc gaggccgaat ttaaaaatgg cgtgggtatg
    11941 accgccacac acttccataa tccgcagagg gcgttcggcg gtgtaagaga gatgtgaagc
    12001 acgttcgcgc agatgctcaa ttaactgcat cacctgttcc ggcgcgcgat attcatcaac
    12061 aaaacgcatt atttttcctc gccatacaac agcgcaccga catccggctc aacgtcaaac
    12121 atgttttgta aggcgtcgag agtgtcgcgt gcttcggctt cattaattac gctcatggca
    12181 aagccaacgt gaaccagtac ccactggccc acgcgcggct gaccgttttc atcgcagctg
    12241 ccgactaacg ttaaatcgac atcgcgctga atgccgcaga cgtcgacttt cgcctggttg
    12301 ccgtcaatgg tgcggatctg gccgggaacg cctatgcaca tcgctgtgtc tccagccagt
    12361 tcagccactg gtccatccct tcgccgctgg tggcggaaat aaggatgatt tcaatttctg
    12421 gattgacttc gcgggcgcag gcgatgcact tctcaacgtc aaagttgaga tacggcaaca
    12481 ggtcaacttt gttgagcagc atcagcgagg cggcggcaaa catatgcgga tatttcagcg
    12541 gtttgtcttc accttcggta acggaaagca ccgctacttt gtgtttttca ccgagatcga
    12601 agctggccgg gcatacgagg ttgccaacgt tttcgataaa cagaataccg ttatcgtcca
    12661 gtggcagacg cggtgcggcg tcggcaatca tctgtgcgtc aagatggcag cctttaccgg
    12721 tgttcacctg aatcgctggt gtaccggtag cgcgaatgcg tgcggcatcg ttcacggttt
    12781 gctggtcgcc ttcaataacc gcgcacggaa cgctgtcttt caggcgcatt aaggtttccg
    12841 tcagcagggt ggttttaccg gaaccagggc tggaaaccag gttgagcacc agttgcttgc
    12901 gggcagcaaa gcgcgcgcgg ttgcgttcag ccagacggtt gtttttgtcc agcacgtcaa
    12961 tttcgacttc cagcatccga cgctggctca tgcccggtgt gtgagtgccc gcttcgccat
    13021 gaccgtaatg caggtcgcct tcttcagtct ggctgggggt aaattcaggc gctttaatgc
    13081 cggtgatttt catctgcggg cgtgccgccg gggcaaatgg cgcgctacga aacgcggaat
    13141 gagggttatg ttcatcaccc tcgatataca ggttgccttc accgcaaccg catgttgtac
    13201 acatcgctca ctcctggtct atttctatcc gccgaatctg taaaccgtcg tctgccacaa
    13261 tctgtagcat gtcaccatga cactgtggac agcggcggac gcgctgggtc agtagcgtca
    13321 catactgttg gcatgtttca caccagcatt cggcctcttg ttcttcgagg tgcagtttac
    13381 aaccttccgc cacgctgccg cggcaaacca gatcaaaaca aaaggcaaga gagctggttt
    13441 cgacacaaga aaatgcgcca attttgagcc agaccccagt tacgcgtttt gcgccgtgtt
    13501 ttgcggcctg ctgttcgatc aattccagtg cccgttggca gagggttatt tcgtgcatat
    13561 cgcctcccat taactattgc cagctacaag caataattgc gccagtgttg attatccctg
    13621 cggtgaataa tgtcgatgat gtcgaaatga cacgtcgaca cggcgacgaa attcatcttt
    13681 agcttaaaaa tctctttaat aacaataaat taaaagttgg tacaaaaaat gcttaaagct
    13741 ggcatctctg ttaaacgagt aacctgacaa tgactatttg ggaaataagc gagaaagccg
    13801 attacatcgc acagcggcat cgtcgcctac aggaccagtg gcacatctac tgcaattcgc
    13861 tggttcaggg gatcacgtta tcgaaagcgc gcctgcatca cgccatgagc tgcgcgccgg
    13921 acaaagaact ctgtttcgtc ctttttgaac attttcgtat ttacgtcacc ctggcggatg
    13981 gctttaacag ccacaccatc gagtattacg tcgaaacaaa agatggcgaa gacaaacagc
    14041 ggattgcgca ggcgcaactg agcattgacg gcatgattga tggcaaggtc aacatccgcg
    14101 atcgcgaaca ggttctggaa cactatctcg aaaaaatcgc tggcgtttac gacagcttat
    14161 acaccgccat tgaaaacaat gtgccggtga atttaagcca actggtaaag ggacaaagcc
    14221 cggcagcatg agctgaggct ttgcccgttt tgcaggcgtt acgcctgttt ggggatgggc
    14281 gtgtcgatga gtgtcgaaaa tgacatttca tcggcatgtt ttcgtcaaaa atgacaatca
    14341 cctgaggaat gcctggtgaa tcgttttgta attgccgact ccacgctctg tatcggctgc
    14401 cacacctgtg aggccgcctg ttcagagacg catcgccagc acggcctgca atcaatgccg
    14461 cgcctgagag tgatgctcaa tgaaaaagaa tctgcgccgc agctctgtca ccactgtgaa
    14521 gatgcaccct gcgcggtggt ctgcccggtt aacgccatca cccgcgttga tggggccgtg
    14581 cagttgaatg aaagtctgtg cgtaagctgc aaactgtgcg gcatcgcctg cccgtttggc
    14641 gcaattgaat tttccggcag ccgtccgctg gatattccgg caaacgccaa taccccgaaa
    14701 gcgccaccgg caccgcctgc tccggcgtgt gtcagcacat tgcttgactg ggtgccaggt
    14761 attcgcgcga tcgccgtcaa atgtgacctt tgtagctttg atgaacaagg tccggcctgt
    14821 gtgcggatgt gcccgactaa agccctgcat ctggtggata acaccgatat cgcccgcgtc
    14881 agcaaacgta agcgtgagct gacttttaac acggactttg gcgatctcac cttgtttcag
    14941 caggctcaaa gtggagaggc taaatgagcg caatttccct gatcaatagc ggcgtggcat
    15001 ggtttgtcgc cgccgctgtt ctggcatttc tcttttcttt tcaaaaagcg ctaagtggct
    15061 ggatagccgg aattggcggc gcggttggta gtctgtatac ggcagccgcg ggcttcactg
    15121 tactgactgg ctcggttggc gtgagcggtg cgctgtcgct ggtaagctac gatgtgcaaa
    15181 tctccccgct taacgcgatt tggctgatta ccctcggtct ttgcggtctg tttgtcagcc
    15241 tctataacat tgactggcat cgccacgcgc aggtgaagtg caacggcttg cagatcaata
    15301 tgttgatggc tgccgccgtc tgcgccgtca tcgccagcaa cctcggcatg ttcgtggtaa
    15361 tggccgaagt catggccttg tgtgcggtgt tcctcaccag caacagcaaa gagggcaaac
    15421 tgtggtttgc gctggggcgt cttggcactc tgctgctggt gattgcttgc tggctgctgt
    15481 ggcagcgtta tggcacgctg gatctgcgcc tgctggatat gcgtatgcaa cagctgccgc
    15541 tcggttccga tatctggctg ctcggttccg atatctggct gctcggtgtg attggctttg
    15601 gcctgctggc tgggattatt ccgctgcacg gctgggtgcc gcaggcacat gctaacgcct
    15661 ctgcgccagc tgccgcgttg ttttctacgg tagtcatgaa aattggcctg ctgggcatgt
    15721 taaccctgtc actgctgggc ggtaatgcac cgctgtggtg ggggatcgcg ctgctggtgc
    15781 tcggcatgat caccgcgttc gtcggtggtc tgtatgcgtt gatggaacac aacatccagc
    15841 gcctgctggc ttaccacacc ctggaaaata tcggcattat cctgctgggg ctggggctgg
    15901 ggctggggct ggcgtaacgg gtatcgcgct cgaacaaccg gcgctgattg ctcttggcct
    15961 ggtcggtggt ctgtaccatc tgcttaacca tagcctgttc aaaagcgtac tgttcctcgg
    16021 ggcggggagc gtctggttcc gtaccggtca tcgcgatatc gaaaaactcg gtggtattgg
    16081 caagaaaatg ccggttatct ccatcgccat gttagtcggg ttgatggcaa tggctgcgct
    16141 gccgccactg aacgggtttg ccggggaatg ggttatctat caatccttct tcaaactgag
    16201 caatagcggc gcgtttgttg cccgtctgct ggggccgctg ctcgctgtgg gtctggcaat
    16261 taccggtgcg ctggcggtga tgtgtatggc gaaagtctat ggcgtcacgt tcctcggcgc
    16321 gccgcgtacc aaagaagccg aaaacgccac ctgtgcgccg ctcctgatga gcgtaagcgt
    16381 agtggcactg gcgatttgct gcgtaattgg cggtgttgct gcgccgtggc tgctgccgat
    16441 gctctctgct gctgtacctc tgcctctgga gcctgctaac accaccgttt ctcaaccaat
    16501 gatcacattg ctgctgattg cctgcccgct gctgccattc atcattatgg cgatttgcaa
    16561 aggcgaccgc ctgccgtcac gttcccgcgg tgcggcctgg gtgtgcggct acgaccatga
    16621 aaaatcaatg gtgattaccg ctcacggttt tgccatgccg gttaaacagg cgtttgcgcc
    16681 agtgctgaaa ctgcgcaaat ggctgaatcc ggtgtcgctg gtgccgggct ggcagtgcga
    16741 aggtagtgcg ttgctgttcc gccggatggc gctggttgaa ctggcggtac tggtggtgat
    16801 tattgtttca cgaggagcct gagaatgagt gttttatatc cgttaattca ggcgctggtg
    16861 ttatttgccg ttgcgccgct gctctccggt attacccgcg tggcgcgcgc ccgtctgcat
    16921 aaccgtcgcg ggccgggcgt gttgcaggag tatcgcgaca ttatcaaact gctgggccgc
    16981 cagagcgttg gcccggatgc ctctggctgg atgttccgcc tgacgccgta tgtgatggtg
    17041 ggcgtcatgc tgactatcgg ttctccgctg ccgcaactgg gcgatttgat caccttactg
    17101 tatctcttcg ccatcgcgcg tttcttcttt gccatttccg gtctggatac cggtagcccg
    17161 tttaccgcta tcggtgcgag ccgtgaagcg atgcttggcg tgctggtcga accgatgttg
    17221 ctgcttggtc tgtgggttgc cgcacaggtt gccggttcca ccaacatcag caacattacc
    17281 gacaccgttt atcactggcc gctgagccaa agcatcccgc tggtgctggc gctctgcgcc
    17341 tgtgcgttcg ccacttttat cgaaatgggc aaactgccgt tcgacctggc ggaagccgag
    17401 caggagttgc aggaaggtcc gctctccgaa tacagcggta gcggctttgg cgtcatgaaa
    17461 tggggtatca gcctgaaaca gctggtggtg ttgcagatgt tcgtcggggt gtttattccg
    17521 tggggacaaa tggaaacctt caccgtcggt ggactgctgc tggcgctggt gattgccatc
    17581 gtcaaactgg tggtcggcgt cctggttatc gcgctgttcg aaaacagtat ggcccgtctg
    17641 cgtcttgata ttactccgcg cattacctgg gctgggtttg gctttgcatt tttagcgttc
    17701 gtctccttgc tggcggcgtg attaaagaga gtttgagcat gtctgaagaa aaattaggtc
    17761 aacattatct cgccgcgctg aatgaggcat ttccaggcgt cgtgctggac cacgcctggc
    17821 agaccaaaga tcagctgact gtcaccgtaa aggtgaacta tctgccggaa gtggtggagt
    17881 ttctctacta caagcagggt ggctggctgt cggtgctgtt tggtaacgac gaacgcaaac
    17941 tgaatggtca ttacgccgtt tattacgtgc tgtcgatgga gaagggcacc aagtgttggg
    18001 taacggttcg cgtcgaagtt gacgccaaca aaccggagta tccgtccgtg acgccgcgcg
    18061 ttccggcggc ggtgtggggc gagcgcgaag tgcgcgatat gtacggtttg attccggttg
    18121 gtctgccgga tgactggccg gatgaacttt atccgctgcg taaagacagc atggattatc
    18181 gtcagcgtcc ggcaccgact accgatgctg aaacctacga gttcatcaac gaactgggcg
    18241 acaagaaaaa caacgtcgtg ccgattggtc cgctgcacgt cacttctgac gaaccgggtc
    18301 acttccgtct gttcgtcgat ggcgaaaaca ttatcgacgc cgactaccgt ctgttctacg
    18361 tccatcgcgg tatggaaaaa ctggcggaaa cccgcatggg ttataacgaa gtgaccttcc
    18421 tctctgaccg tgtgtgcggg atctgcggct ttgcccacag caccgcctac accacgtcgg
    18481 tggaaaacgc gatgggtatt caggtgccag aacgcgcgca gatgatccgc gccattctgc
    18541 tggaggtgga acgcctgcac tcgcatctgc tcaacctcgg cctcgcctgt cactttaccg
    18601 ccttcgactc cggctttatg cagttcttcc gcgtgcgtga aacctccatg aaaatggcag
    18661 agatccttac cggtgcgcgt aaaacctacg gcctgaacct gatcggcggg attcgtcacg
    18721 atctgctgaa agatgacatg atccagaccc gccagctggc gcaacaaatg cgtcgtgaag
    18781 tgcaggagct ggtggatgtg ctgctgagta cgccgaacat ggaacaacgc accgtcggca
    18841 ttggtcgtct ggacccggaa atcgcccgcg atttcagtaa cgttggcccg atggttcgcg
    18901 ccagcggtct cgcccgcgat actcgcgccg atcacccgtt tgtcggttat ggcctgctgc
    18961 caatggaagt ccacagcgag cagggctgcg acgttatttc ccgtctgaaa gtgcgaatca
    19021 acgaggtcta taccgcgctg aatatgatcg actacggtct ggataacctg ccgggcggtc
    19081 cgctgatggt ggaaggcttt acctacattc cgcaccgttt cgcgctgggc tttgccgaag
    19141 cgccgcgcgg cgacgatatc cactggagca tgaccggcga caaccagaag ctgtaccgct
    19201 ggcgctgccg tgccgcgacc tacgcgaact ggccgaccct gcgctacatg ctgcgtggca
    19261 ataccgtttc cgatgcgccg ctgattatcg gtagcctgga cccttgctac tcctgtaccg
    19321 accgcatgac cgtagtcgat gtgcgtaaga agaagagcaa agtggtgccg tacaaagaac
    19381 tcgagcgtta cagcattgag cgtaaaaact cgccgctgaa ataaggaatc gccatgttta
    19441 cctttatcaa aaaagtcatc aaaaccggca cggcgacctc gtcttatccg ctggagccga
    19501 ttgcggttga taaaaacttc cgtggtaagc cagagcagaa cccgcagcag tgcatcggct
    19561 gcgcggcttg cgtcaatgcc tgcccgtcaa acgccttaac ggttgaaact gacctcgcca
    19621 caggagagct ggcgtgggag tttaatctcg ggcgctgcat cttctgtgga cgctgcgaag
    19681 aagtttgccc gacggcggcg atcaaactgt cgcaagagta cgaactggcg gtgtggaaga
    19741 aagaaaactt cctgcaacag tcccgcttcg cgctgtgcaa ctgccgcgtc tgcaatcgtc
    19801 ctttcgccgt acagaaagag atcgactacg ccattgcgct gcttaagcac aatggtgaca
    19861 gccgcgcgga aaaccaccgc gaaagctttg agacttgccc ggaatgtaag cgccagaaat
    19921 gcctggtgcc gtccgaccgt attgaactga ctcgccatat gaaagaggcc atctgatgag
    19981 caatttatta ggcccccgtg acgccaacgg cattccggtc ccgatgacgg tggatgaatc
    20041 catcgccagc atgaaggcgt cgttactgaa aaaaatcaaa cgttctgcct atgtttaccg
    20101 cgtggactgc ggcggctgca acggctgcga aatcgaaatt ttcgccacgc tttctccgct
    20161 gttcgatgca gaacgcttcg gcattaaagt cgttccttca ccgcgtcatg cggatatttt
    20221 actgtttacc ggcgcggtca cccgtgcaat gcgatcccct gcgctgcgtg cgtggcagtc
    20281 cgcgccggac ccgaaaatct gtatctcata cggtgcctgc ggtaacagcg gcgggatctt
    20341 acacgatctc tactgcgtgt ggggcggtac ggataaaatt gtccctgtgg atgtttatat
    20401 ccctggctgc ccgccaacgc ctgccgccac gctgtacggc tttgcaatgg cgctcggcct
    20461 gctggagcag aaaattcacg cccgtgggcc gggtgaactg gatgaacaac cggcggagat
    20521 cctgcatggc gatatggtgc agccgctgcg cgtgaaagtg gatcgcgaag cacgtcgcct
    20581 ggcgggttat cgttacggtc gtcagattgc cgatgattac cttacacagt tagggcaggg
    20641 cgaagaacag gttgcacgct ggctggaagc ggaaaacgat ccgcgtctga acgagattgt
    20701 cagccatctg aatcatgttg ttgaagaggc gcgtatccga tgagtgaaaa ggtggtgttc
    20761 agtcaactga gccgtaaatt tattgatgag aacgatgcca cgcccgccga ggcgcagcag
    20821 gtggtctatt acagcctggc gattggtcac caccttgggg ttatcgattg cctggaagcg
    20881 gcgctcacct gcccatggga tgaatatctg gcatggattg ccactctgga ggcgggcagc
    20941 gaagcccgcc acaaaatgga aggcgtaccg aaatatggcg agatcgtcat cgacattaac
    21001 catgtgccga tgctggccaa cgcattcgat aaagcccggg cagcgcaaac ttcgcagcag
    21061 caggaatgga gtacaatgct gttaagtatg ctgcatgata ttcatcagga aaacgccatc
    21121 tatttgatgg tgaggagact gcgtgactga cgttttactc tgtgttggca atagcatgat
    21181 gggcgatgat ggcgctggcc cgctgctggc ggaaaagtgc gccgccgcgc cgaaaggtaa
    21241 ctgggtggtg attgacggcg gtagtgcacc ggaaaacgac atcgtcgcca tccgtgaact
    21301 gcgcccgaca cgactgctga ttgtcgacgc cacggatatg gggctaaacc ccggcgaaat
    21361 ccgcatcatc gacccggatg atatcgccga gatgtttatg atgactaccc ataacatgcc
    21421 gttgaattac cttatcgacc agttaaaaga agacattggt gaagtgattt tcctcggcat
    21481 tcagccggat atcgtcggct tttactaccc gatgacccag ccgattaaag atgcggtaga
    21541 aaccgtttat caacgactgg aaggctggga aggaaatggt ggatttgcgc agttagcggt
    21601 ggaagaagaa taattctcca tcagggaatt cagggaattc agggaattca gggaattcag
    21661 ggaattgaac atcagaacag ggatgtgcgc ttttttattg agcttagtgc tgcctgcaca
    21721 ggccaccagc tttaccgaat atctgccaat gagtgacagc gaatacgcgc aaaagcgggc
    21781 gctgaaacct ctcctgacca tgccttatga tgccgagcaa acctggcatt ttaggaaagt
    21841 gggcgttgct ggcgtgacgc ttgagaagat gccaaatgaa gaagattact ggcaattaac
    21901 cgctaaagac cgcgccggta aatcctggac tgtgcctgtg ggtatgctgg caaacatagc
    21961 gggcaaaggg caactctatc gcgccgatct tgatcgtaac ggcattcagg atctggttat
    22021 ctggttacca agtaccggta atggtctggc tccttatgca catttaattc ttatgacgtt
    22081 cacgcgcgaa ggccgtccct gtgtctttga acccagggga ttctacaccg caagcaaaac
    22141 tggcgtagat gatttgctgg atttgcaagg caatggtcac acgcaattgc tggatatgca
    22201 gtttaattct ggttactgga taaccagtct gtatcaggta aaagacgcca aatggcagcg
    22261 tgttcacggc tggtttgaca agttaagcta tcccgcgctc acgcgtttta cttatacccc
    22321 caaccgtaaa ctcgtcctca aacccatcgc cgggcgcgac ccgcaaacgg aagatctggc
    22381 gcagacgcag cgttgcttga taaaggggga tgtgcttgag gggggaacca gaactgactt
    22441 tttgcagagt ggctatgagg attttggggg taattatatc gcgcacaagt gtttacatat
    22501 tgtgaacttg gatttgatga gctatagttt ccgaacacat aaggatagaa ctcgcagatg
    22561 tggctgttta cttactataa ggaagttaaa acttaaaaat gcacatcatt tctaaagcgc
    22621 catttgaaga gtctgcgcgt aaatatccta acgacgctct ggctcttcag gcattgtatc
    22681 gggttattaa ggaaacggat ttttctacgc cagaagagat gcgaacggcg tttcccaacc
    22741 tcgacaattt taaatatcgc aacaaatggt acgttctgga tgtaggtggg aacaacctag
    22801 gggtgattgc ctacataaac tttgttaata agcggttctt tgtgaagcac ataactaatc
    22861 acgccgagta tgacaaactg acacgttact acagagagaa caaagaatga ctgctaatgc
    22921 tgcacacgct gttaaagcaa caagagaact ggtcaatgca gtcccctttc tgggaggtag
    22981 tgactcagaa gatgattatc gtgaagcact ggaactggtc gaatatttga ttgaagaaga
    23041 tgacaccaat ccacttatcg actttctggc gagccgcatt gcagaatatg aaaacaacaa
    23101 ccaagagttt gcagagttcg acaaagccgt tgcggcaatg cctgttggcg tggcgttact
    23161 tcgcacgctg attgatcaac ataatctgac ctatgcggat ttgaagaacg aaatcggctc
    23221 taaatccctg gtcagtcaga ttttgtctgg acaacgatcc ctgacgatat cccacattaa
    23281 ggcactttca gcccgatttg gcgttaagcc ggaatggttt ctttgatttt tgactaatgc
    23341 caaatgctaa attgcccgat gcgctgcgct tatcgggcct acatgtcgca cgaactatgt
    23401 aggccggata aggcgttcac gccgcatccg gcactgttac ttactctaaa tcttccccat
    23461 tactggcaat cactttttta taccaccaga acgatttctt acgtgtgcgc gtcagcgtgc
    23521 cgttgcctgc gtcgtcacgg tctacgtaga caaaaccgta gcgtttgctc atttcacccg
    23581 tagaggcgga aactaaatca atacagcccc atgtggtgta gcccatcagc ggaatgccat
    23641 ctgcaatcgc ttcgcctatt gcgcggatat gttcgcgtaa atagctgatg cgatagtcat
    23701 cgttaatctc gccattggca gcaagttcat cttttgcgcc gaggccgttt tccaccagaa
    23761 acagcggctt ctgataacga tcgtacatca tattcatggt gatacgcaga ccgagtggat
    23821 caattcccca gccccagtcg ctcacttgca gatacgggtt acgcagcgat ttcaccacgt
    23881 tcgccgcact gctgttgttg gcgttcatct ccgccgaggc gcagcgcgag gcgtaatagc
    23941 tgaaagagac aaaatcgacg gtgtttttca ggatctcatc atcgcccagc gctttatcta
    24001 tggttacccc tttttcgcga aatacgcggg cagagtaagc cggatacgcg ccgcgcgcct
    24061 gcacatcgat aaaaaacagg ttttcccgat ctttctccag cgccgcccag acatcttccg
    24121 gtttgcagct gtaagggtag aagttaccgc ccgccagcat acagcccacc tgattttgcg
    24181 ggttaacctc atgggcgatt ttggtggcta gcgcactggc aaccagctgg tgatgcgcgg
    24241 cctgatattt cacctgatcc tgattttcac cttcttcaaa caccagaccc gcgccggaga
    24301 acgggctatg caacataatg ttgatttcat tgaaggttag ccagtatttc accagaccat
    24361 caaatgcttc aaagcaggtt ctggcgtagc ggctgaaaaa ctccaccagc ttgcggttac
    24421 gccaggagcc atattcggtg accagatgca tcggcacatc gaagtggcac aacgtgacca
    24481 gcggttcgat accgtacttt ttacactctt caaagacagg acgataaaaa gcaatgccct
    24541 gctgattggg cgtgatttca tcgtcctgcg gaaagagacg gctccaggca attgaggtac
    24601 ggaaaacctt gaatcccatc tctgccatca gggcgatatc ttctttataa cgatgataaa
    24661 aatccgtcgc ctcatggctg ggataaaact cgtcatcgcg caactgaaaa cgtttttcca
    24721 gccccagttt caccgccatt cgatgctcgc cgtgtgggat catatcgacg gtggtcagcc
    24781 cttttccgcc ttcacggaac gcaccttcag actggttggc ggcaagcgcg ccgccccata
    24841 aaaaaccttc tggaaatact gacattttca tcctcaatta agacttactt ctttggcgac
    24901 ggtcggttga tgctttcgcg cctgggcagc cgcttcctca acaggaatat cctcaaagcc
    24961 gagcaacagt gtgaggataa acgacagcac caccgccagc gccatgaccg cgaacaccca
    25021 gacgatgctc attggattcg ccggatcgaa gaattgcacg ctggtaaata gccccgg
//
LOCUS       DADLTF010000049        24860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.049, whole genome shotgun sequence.
ACCESSION   DADLTF010000049 DADLTF010000000
VERSION     DADLTF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.049"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            72..353
                     /locus_tag="JN749_001890"
     CDS             72..353
                     /locus_tag="JN749_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7368326.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1396
                     /locus_tag="JN749_001891"
                     /pseudo
     CDS             319..1396
                     /locus_tag="JN749_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1789..2130)
                     /locus_tag="JN749_001892"
     CDS             complement(1789..2130)
                     /locus_tag="JN749_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HAY7368327.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2143..3015)
                     /gene="copD"
                     /locus_tag="JN749_001893"
                     /pseudo
     CDS             complement(2143..3015)
                     /gene="copD"
                     /locus_tag="JN749_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="JN749_001894"
     CDS             complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="JN749_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HAY7368328.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3532..3762
                     /locus_tag="JN749_001895"
     CDS             3532..3762
                     /locus_tag="JN749_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HAY7368329.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3864..4520
                     /locus_tag="JN749_001896"
     CDS             3864..4520
                     /locus_tag="JN749_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HAY7368330.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4544..5206
                     /gene="exoX"
                     /locus_tag="JN749_001897"
     CDS             4544..5206
                     /gene="exoX"
                     /locus_tag="JN749_001897"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HAY7368331.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="JN749_001898"
     CDS             complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="JN749_001898"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HAY7368332.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7472..8131)
                     /locus_tag="JN749_001899"
     CDS             complement(7472..8131)
                     /locus_tag="JN749_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HAY7368333.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JN749_001900"
     CDS             complement(8458..8814)
                     /gene="yebF"
                     /locus_tag="JN749_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HAY7368334.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JN749_001901"
                     /pseudo
     CDS             complement(8881..9171)
                     /gene="yebG"
                     /locus_tag="JN749_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9305..10483
                     /gene="purT"
                     /locus_tag="JN749_001902"
     CDS             9305..10483
                     /gene="purT"
                     /locus_tag="JN749_001902"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HAY7368335.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10538..11179)
                     /locus_tag="JN749_001903"
     CDS             complement(10538..11179)
                     /locus_tag="JN749_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAY7368336.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11216..13027)
                     /gene="edd"
                     /locus_tag="JN749_001904"
     CDS             complement(11216..13027)
                     /gene="edd"
                     /locus_tag="JN749_001904"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HAY7368337.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="JN749_001905"
     CDS             complement(13262..14737)
                     /gene="zwf"
                     /locus_tag="JN749_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAY7368338.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /locus_tag="JN749_001906"
     CDS             15075..15944
                     /locus_tag="JN749_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7368339.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="JN749_001907"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="JN749_001907"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAY7368340.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="JN749_001908"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="JN749_001908"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7368341.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="JN749_001909"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="JN749_001909"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HAY7368342.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLLFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="JN749_001910"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="JN749_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HAY7368343.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="JN749_001911"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="JN749_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HAY7368344.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="JN749_001912"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="JN749_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HAY7368345.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="JN749_001913"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="JN749_001913"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAY7368346.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="JN749_001914"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="JN749_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAY7368347.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24538..24603)
                     /locus_tag="JN749_001915"
     CDS             complement(24538..24603)
                     /locus_tag="JN749_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368348.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..>24860
                     /locus_tag="JN749_001916"
     CDS             24674..>24860
                     /locus_tag="JN749_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368349.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTIT"
BASE COUNT         6170 a         6293 c         6103 g         6294 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaaatt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg ggggattatc taatcagtgc
     1141 cgggattaga aaaaacagcc ctgacggcag cattcacccg gacggcctga caaagaaatt
     1201 tgtcgcagcc agaaaattaa caggtatcct gttcagtgaa aacccaccaa cttttcacga
     1261 aatcagaagc ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg
     1321 tctacttggc cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac
     1381 gtacttactg ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata
     1441 accaaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt
     1501 tgcaactaat tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt
     1561 ccagggaaat ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta
     1621 gttgccttgc cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg
     1681 gtcaataaaa agcgcggtgg tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa
     1741 gaactgaggc ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt
     1801 ttcagctcgt tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc
     1861 aatctgaatc accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc
     1921 gatatcgttt aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc
     1981 cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc
     2041 aaaacgactc acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa
     2101 cagaaacgcc agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt
     2161 cgcaagcagg ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat
     2221 ccacacaaac cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata
     2281 acgattagcc agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag
     2341 ttggccctag taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac
     2401 gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc
     2461 ctgaatagcc tggtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag
     2521 taatccccca aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat
     2581 tttcgctgtt accccttcat tcagcgtcgc atgccccact cccgccagca gaataaattg
     2641 cgcggtagtg agcataaaca acaaacgtgg catattccgc ggttgcataa gggcgacgat
     2701 caacgttacg agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa
     2761 aacagcctgc caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc
     2821 ctgaacggcg agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa
     2881 acgcagagaa cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata
     2941 cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca
     3001 ggtaaacgcc agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc
     3061 gtccaccgaa acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag
     3121 tggaacaatc agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt
     3181 ttcgtttttt ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa
     3241 gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg
     3301 cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag
     3361 cgcgtagcga agggagcgtg cagttgaagc catattatct attccttttt gtaataactt
     3421 ttttacagag cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca
     3481 tccaggctca ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag
     3541 aatctggcta aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc
     3601 ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa
     3661 cagccagaac atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg
     3721 gtcaatctgt cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca
     3781 ctttcttaca taacgcctgc taaattatga gtattttcta aaccgccccc ataatttgca
     3841 gtcattttga aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc
     3901 ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg
     3961 ccaggcaaca atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt
     4021 cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg
     4081 caacgacctg gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta
     4141 ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc
     4201 atggtgcatg tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag
     4261 gtatggatat ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg
     4321 acctgctggc gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc
     4381 tgatggcaaa tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc
     4441 gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg
     4501 atatcattcc attacgctag gctttttcgg cctggagcat gccatgttgc gcattatcga
     4561 tacagaaacc tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga
     4621 cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca
     4681 agcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
     4741 tgtgatccca cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg
     4801 ccgcgtactg cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
     4861 gtggccaggg ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca
     4921 gacaccgccg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
     4981 gcttatcgat attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg
     5041 acgtccgtcg ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga
     5101 cgttgccgaa cgcgatccgg gctatctgcg ctggttattt aataacctgg acagcatgag
     5161 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagt
     5221 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
     5281 cgatttaaag cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa
     5341 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
     5401 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
     5461 gacgttgtca tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt
     5521 tccccactct tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac
     5581 aacatcaaca aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat
     5641 tgcaacgccc atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga
     5701 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
     5761 cttcttcaga aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac
     5821 atggacaatg gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc
     5881 ggcatcaata cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt
     5941 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
     6001 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccgggcactt ctgtttgttt
     6061 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat
     6121 ggaagaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
     6181 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
     6241 gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
     6301 aaacccttcc agcatgatgt tttcacgtgg cggaattaac tcttcccact gttgctcatc
     6361 acgcatacgg gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa
     6421 acgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
     6481 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
     6541 cagatgaatg acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc
     6601 gtctttttct tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca
     6661 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
     6721 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
     6781 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
     6841 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
     6901 tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga
     6961 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
     7021 attttttatg tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa
     7081 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
     7141 ttgcaggtag tccaggactt ctggctgaga acgcgtatcg tcccgcagcc agtagtaatt
     7201 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     7261 catgttattg ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accggggatg
     7321 caagcgaaac agggtcgtca ttgcttagat gatgacaggt aatggcgcgg atatcgaatg
     7381 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga tagcccgaaa
     7441 tagtcaactt caggctatcc agagagcgga attattccgc caaagtgcgt ttttgctgtt
     7501 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgcg tcacccagtg
     7561 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
     7621 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
     7681 gttgttcgat tgcctgctca atgagtacac gcccctgctc ttccacgccg gcttcacgca
     7741 attgctggtc gattgccgca cgttcattgg catcaatatg accatcactt ttagcggcaa
     7801 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
     7861 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
     7921 tacccgctac cgctccgccg ccaaccagta acgcgttggt gccatatttc gtaagaagtt
     7981 tacgtgctga tttatttgcg accagcagcc cagccagtcc gcctaatgcg cctggcacta
     8041 acagtttgcc caatccctga tccgcagacg aggaggtaga agaactgctt tgcccaagca
     8101 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
     8161 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
     8221 agcggggcgc taggccgtta ttcaaagaaa ttcgcgccag gtaaggtatt tactgtatat
     8281 caggtgataa gggtatttta ggtgaaagta gtttgtcgtt ttgagtggca ttgtttgatg
     8341 ctgattgccc gatgcgacgc tgttgcgtct tctcaggcct acgattgtgg tgcgttctgg
     8401 gacggacaag gcactcgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
     8461 acgccgctga tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc
     8521 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttac cctgaatgtc
     8581 ctgcaaactg acccaggcca cgggatcggc ctgaccgaca attttttgat catctgccca
     8641 gcgcgccacg cgattttgtt gataatcacg ttttacgctc gcggcaattc cggtagcatc
     8701 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
     8761 tgatgcgcag gcagaaacca acaaccgccc taaaaacgcc cctctttttt tcatgttttt
     8821 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
     8881 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacca gtggggacgg
     8941 taatttgccg gttttcagaa tggtgctcag catatctttt tgttctgcca gccatagcga
     9001 aagggcttca cgttgctcgt cttccatttg cactggagaa tttgtcagct aggtgtcaag
     9061 cagatccgcc gtatcaagca ttttgtcata agcatcggct tcctttttgc tggtaaacga
     9121 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
     9181 ctcataataa ctgtgatttt atacagtata tttcttttcg gttgagaaat caacatcagc
     9241 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
     9301 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
     9361 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
     9421 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
     9481 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
     9541 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
     9601 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
     9661 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcaaaag
     9721 ccttttccgc gaggcggttg ccgccattgg ctatccctgc attgtaaaac cggtgatgag
     9781 ctcttccggc aaggggcaga cgtttattcg ctctgcagag caacttgctc aggcatggga
     9841 atacgctcag caaggcggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
     9901 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
     9961 accagtaggt catcgccagg aagatggcga ctaccgtgaa tcctggcaac cacagcaaat
    10021 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    10081 cggttatggt ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    10141 ggtctcccct cgtccacatg ataccgggat ggtgacgtta atttctcaag atctctcaga
    10201 gtttgccctg catgtacgtg ccttcctcgg acttcctgtt ggcgggatcc gtcagtatgg
    10261 tcctgcagct tctgccgtta ttctgccaca actgaccagt cagaatgtca cgtttgataa
    10321 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    10381 tggcagccgt cgtctggggg tggtactggc tacttcagag agtgttgttg acgccattga
    10441 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaacgct
    10501 acaaaaatgc ccgatcctag atcgggcatt ttgactttta cagcttagcg ccttctacag
    10561 cttcacgcgc cagcttagta atgcggtcgt aatcgcccgc ttccagcgca tctgccggaa
    10621 ccagccagga accaccgatg cacagcacgc ttttcagtgc caggtagtca cggtagttag
    10681 ccggagaaat accacccgtc gggcagaaac ggacctggga gaacggaccc gcgatcgcct
    10741 gcagggcttt cacgccgccg ttagcttcag ccgggaagaa tttgaactct ttcaaaccgt
    10801 agtccatacc cagcatcagt tcggaaacag tgctgatccc cggaatcaga ggaatagtcc
    10861 cttcggtagc agctttcagc agcggctcgg tcagacccgg gctaattgcg aactgtgcac
    10921 ccgcttcagt gacttctgcc agctgctgtg gattcagcac cgtaccggca cccacaatcg
    10981 cttcaggcac ttctttggcg atagcacgga tagcgtcaac tgcacactcg gtacgcagag
    11041 tcacttccag aacgcgcacc ccaccagcaa ccaacgcttt tgccatcggc accgcgtgtt
    11101 ccagtttttt taccacgata accggtacaa ccgggccggt ggtcaggatt gattctgcac
    11161 ttgttttcca gtttttcatc agagttttct ctcgcctgat tacaaatttg tcgtcttaaa
    11221 aagtgataca ggttgcgccc tgttcggcac cggacagttt ttcacgcaag gcgctgaata
    11281 attcacgtcc tgttctcacg cgtgacgcgc tcaggtcagg aatgtgcggt tcgcgagcag
    11341 ccagttccgc ttcgtctacc agcagtgtca gttcgcctgt ctgtccattc acacgaatga
    11401 tgtccccgtc gcgcactttt gccagcagcc cgccatcgta ggcttctggt gttacgtgga
    11461 tagctgacgg cactttacct gaagcgccgg agagtcgtcc atcggtaact aacgcaattt
    11521 tgaaacaccg gtccaataat acaccaagtg gcggcatgag tttatgtaat tctggcattc
    11581 cgttcgcttt tggcccctga tgacggacaa caacgacaca atcgcggtcc agcaaacccg
    11641 cttcaaaggc cggcataacg tcatgctggc tttcaaaaac aaccgctggc gcttcaatca
    11701 cctggttctc aaccggcacg gcagaggttt tcataaccgc acggcccagg ttaccgctta
    11761 acacttttgt cccaccatga tgagagaaag gttgttcgaa ggaagcgatc acattgctgt
    11821 cgagtgattt ttccgcccct tcccgccagt ccagttcacc attattcagc catggttcaa
    11881 gggtataacg aaacagacca aagcctgcca ccgtattgac atcttcatgc agcaggcctg
    11941 ctttgagcag ttcacgcacc agaaccggta cgccacctgc cgcctggaag tggttaatat
    12001 cggccggacc gttcgggtag agacgtgcca tcagcggtac aacatcagaa aggtcagaga
    12061 agtcatccca gttaatctga ataccggccg cgcgcgccat cgccaccagg tgcatggtgt
    12121 ggttagtgga accaccggtc gccaacagtg caacgatacc gttcaccacc actttctcat
    12181 cgatcatctt accgatcggc atccattcat taccattacc ggtcatgcgt gtaacctgac
    12241 gcgcagcggc agcggtcaaa gcatcgcgca gcggagaatc cggatgaaca aaagaagagc
    12301 ctggcaactg catccccata aattccacca ccatctggtt ggtgttggca gtaccgtaga
    12361 aagtacatgt tcccggcgca tggtaagacg cggcttctga ctccagtaag gccatgcggt
    12421 ccactttacc ttcggcataa agctggcgaa tacgcacttt ttctttattt ggcaaaccgc
    12481 ttgccatcgg tccagacggc acaaacaccg caggcaaatg accaaacgac agggctgcca
    12541 tcgtcagacc cgggacaatc ttgtcgcaca caccgaggaa cagagcacca tcaaacatgt
    12601 tatgggacag ccccaccgcc gcagacatcg ctatcacttc gcggcttagc agcgacaatt
    12661 ccattccatc ctgcccctgg gtgacaccat cacacatcgc cggaacaccg cccgcaacct
    12721 gaccaaccgc attcgcttca tgcagggctt tacgaatgat ttctggatag tgttcataag
    12781 gctggtgcgc ggagagcatg tcgttatagg aggtgatgat ggcgatattg ttacgcaaca
    12841 tgcttttcaa agaggctttg tcttctggct ggcaggcagc gaaaccgtgt gccaggttac
    12901 cgcatgccaa ctgcgaacga tgaacggtcg acgttttcgc ttgttctatc cgggcgagat
    12961 aagcagagcg agtctcgcgc gaacgttcaa tgattcgatt tgttacgcgt aacaattgtg
    13021 gattcataaa ggctcctgaa attgagttgt cagagcagga tgattcacaa cgcgtttcat
    13081 tcagaggatt tatgagtgaa acgcctgtaa ccggagctca tagggcaaaa acgtttcagt
    13141 cagtgtaata aaaaaagcct cgtgggtgaa tccgcacgag gcctgaaagt gtaaaaattg
    13201 ttctacaatc tgtgcaagat tatgttaccg gtaaaataac cataaaggat aagcgctgat
    13261 attactcaaa ctcattccag gaacgaccat cacgggtaat catcgccacc gaggcaacgg
    13321 gtccccaggt tccggcctga tacggtttcg gcgcatcatt gtccatcgcc cacgcctcag
    13381 taatggagtc tacccatttc caggcttctt ccacttcgtc gcgacgtaca aacagtgcct
    13441 gaataccacg catggtttcc agcagcaaac gttcataggc atccgccaga tgcgtctgat
    13501 taaaggtttc tgaatagctc agatccagct tggtgatttg caggttatgt ttgtggtcaa
    13561 ggccaggaac tttattcagt acctggatat ccacgccttc atcaggttgc agacggatag
    13621 tcagtttatt ctgcggcaga tcctgccacg actctttaaa cagattcagt tcaggtgttt
    13681 tgaaatagac cacgacttca gaacatttgg tcggcagacg tttaccagta cgcaggtaga
    13741 atggcacacc ggcccagcgc cagttatcaa tgtcgacgcg gatcgccacg aaggtttctg
    13801 tattgctact tttgttcgcg ccctcttctt ccagatatcc cggcactttt ttgccctggg
    13861 cgaagcccgc agtatattgc ccgcgtacgg ttttttcgcg tacgttggag cggtcgatgc
    13921 ggcgcaggga cttcagtact ttcacttttt catcgcggat gctgtctgcg ctcaggtcag
    13981 atggcggaga catcgcaatc atgcagagaa tttgtagcag gtggttctga atcatgtcac
    14041 gcatctgacc ggctttatca aaatagcccc agcgcccttc gatccccact tcttctgcca
    14101 cggtaatctc aacatgatca atggtgcgat tgtcccagtt attcacaaac agggagttag
    14161 caaaacgcag cgccaacagg ttcagcaccg tttctttacc aagatagtgg tcgatacggt
    14221 aaacctggca ctcctcgaag tattcgccaa cctgatcatt gatttcctgc gaggtcgcca
    14281 gcgacgtccc cagcggtttc tccatgacta cgcgtgccgg tttagcattc agttttgcct
    14341 cgccaagccc tttgcaaatt gcgccaaaag tgctgggcgg catggcaaag tagttaatag
    14401 tgatacgatt tttttgatcc agcatcgcgc cgagacggtt gaatgcagca gtgtcattga
    14461 catcgagatt acaaaaatcc agacgtgcac tcagggtgtc ccataaacct tcatcaatgg
    14521 tttctttcat gaaagtttcg agcgcctcgc ggacaacttt ggtatatgcc gctttatccc
    14581 agtcagcacg ccctacgccg ataatccggg tgtccgggtt gagctgaccg gctttttcca
    14641 gttgatacag ggaaggcagc aatttacgac gcgcaaggtc gcctttcgcg ccgaaaatga
    14701 ccaggtcaca ggcctgggct gtttgcgtta ccgccatgtc attctcctta agttaactaa
    14761 cccggtactt aagccagggt atacttgtaa ttttcttacg gtgcactgta ctgcttttcc
    14821 gagcttgcga aaactgtaaa cgcttatcca cccgtgcgat tacgggaaaa gcgcgcaaag
    14881 tgcggcaaaa ctgataaaaa aatcatcgtt ttccattcgg taaaaatctg acactgatca
    14941 tgttatgaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct aggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctag tgattgtcac ccagggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aattatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaacagtag ctgcggcaca atctcttttg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagcg agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaag cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctggcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca
//
LOCUS       DADLTF010000050        24798 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.050, whole genome shotgun sequence.
ACCESSION   DADLTF010000050 DADLTF010000000
VERSION     DADLTF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.050"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(51..884)
                     /gene="fdhD"
                     /locus_tag="JN749_001917"
     CDS             complement(51..884)
                     /gene="fdhD"
                     /locus_tag="JN749_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HAY7368350.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1078..4128
                     /gene="fdnG"
                     /locus_tag="JN749_001918"
     CDS             1078..4128
                     /gene="fdnG"
                     /locus_tag="JN749_001918"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1663..1665,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7368351.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMFSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGITLTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4141..5043
                     /gene="fdxH"
                     /locus_tag="JN749_001919"
     CDS             4141..5043
                     /gene="fdxH"
                     /locus_tag="JN749_001919"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7368352.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5040..5675
                     /gene="fdoI"
                     /locus_tag="JN749_001920"
     CDS             5040..5675
                     /gene="fdoI"
                     /locus_tag="JN749_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HAY7368353.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5672..6601
                     /gene="fdhE"
                     /locus_tag="JN749_001921"
     CDS             5672..6601
                     /gene="fdhE"
                     /locus_tag="JN749_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HAY7368354.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6931..7173)
                     /locus_tag="JN749_001922"
     CDS             complement(6931..7173)
                     /locus_tag="JN749_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368355.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7391..7609)
                     /locus_tag="JN749_001923"
     CDS             complement(7391..7609)
                     /locus_tag="JN749_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HAY7368356.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8288..9433)
                     /locus_tag="JN749_001924"
     CDS             complement(8288..9433)
                     /locus_tag="JN749_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368357.1"
                     /translation="MCERMKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSA
                     KDVGPDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPK
                     NNKPKTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAH
                     SHRSVALAKTVAAPIMATVVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQS
                     DISVRNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGC
                     KSAKDRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKNLF
                     DIRVLHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            complement(9591..9929)
                     /locus_tag="JN749_001925"
     CDS             complement(9591..9929)
                     /locus_tag="JN749_001925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368358.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="JN749_001926"
                     /gene_synonym="yiiD"
     CDS             complement(10519..11460)
                     /gene="fabY"
                     /locus_tag="JN749_001926"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HAY7368359.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="JN749_001927"
     CDS             complement(11505..11942)
                     /gene="dtd"
                     /locus_tag="JN749_001927"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAY7368360.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11939..12811)
                     /locus_tag="JN749_001928"
     CDS             complement(11939..12811)
                     /locus_tag="JN749_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HAY7368361.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="JN749_001929"
     CDS             complement(12805..13404)
                     /gene="yihX"
                     /locus_tag="JN749_001929"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HAY7368362.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13503..14287)
                     /locus_tag="JN749_001930"
                     /pseudo
     CDS             complement(13503..14287)
                     /locus_tag="JN749_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            complement(14321..15217)
                     /gene="yihV"
                     /locus_tag="JN749_001931"
     CDS             complement(14321..15217)
                     /gene="yihV"
                     /locus_tag="JN749_001931"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HAY7368363.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGVKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            15385..17040
                     /gene="yihU"
                     /locus_tag="JN749_001932"
     CDS             15385..17040
                     /gene="yihU"
                     /locus_tag="JN749_001932"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HAY7368364.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARASGGFA
                     MLAVDQREAMRMMFAAAGAPAPVADSVLTDFKVNAAKALSPYASAILVDQQFCYRQVV
                     EQNAIAKSCAMIVAADEFIPGNGIPVDSVVIDRKINPLQIKQDGGKALKLLVLWRSDE
                     DAQQRLDMVKEFNGLCHSHGLVSIIEPVVRPPRRGDKFDREQAVIDAAKELGDSGADL
                     YKVEMPLYGKGPQQELLCASQRLNDHINMPWVILSSSVDEKLFPRAVRVAMTAGASGF
                     LAGRAVWASVVGLPDNELMLRDVCAPKLQQLGDIVDEMMAKRR"
     gene            17057..18298
                     /gene="yihS"
                     /locus_tag="JN749_001933"
     CDS             17057..18298
                     /gene="yihS"
                     /locus_tag="JN749_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HAY7368365.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            18412..19338
                     /locus_tag="JN749_001934"
     CDS             18412..19338
                     /locus_tag="JN749_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HAY7368366.1"
                     /translation="MSLIKVPCMQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTF
                     QGCHLVIPHKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGL
                     LAWRDWQISELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTV
                     AAPYGVGIHPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKK
                     IAATKIDHTFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMS
                     CPPNAFNSGIDLLLLEPGKPHRLFFNIYGQRK"
     gene            19537..21573
                     /gene="yihQ"
                     /locus_tag="JN749_001935"
                     /pseudo
     CDS             19537..21573
                     /gene="yihQ"
                     /locus_tag="JN749_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            21619..23004
                     /locus_tag="JN749_001936"
     CDS             21619..23004
                     /locus_tag="JN749_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368367.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            23047..24450
                     /locus_tag="JN749_001937"
     CDS             23047..24450
                     /locus_tag="JN749_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368368.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            24518..>24798
                     /locus_tag="JN749_001938"
     CDS             24518..>24798
                     /locus_tag="JN749_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368369.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         6138 a         6380 c         6091 g         6189 t
ORIGIN      
        1 cgaaatgaat aagtcctgca atttaatata tatcgcagga cttattttaa ttaattgctc
       61 aaacgctgcg gatgggtata aaccgttgcc cgacccggtt tacaaaaacc taccagtgtc
      121 agattacagc gctcagccac ttctacagca agcgtggtcg cggcagacac cgcaaacaaa
      181 atctctacgc cgcacatcgc cgacttttgc accatttcat aactggcacg actggaaacc
      241 agtaccgcac cttgctgcca gctttcccct tcttgtgacc ggcggcctaa cagtttgtcc
      301 agcgccacat ggcgacccac gtcttcatgc ccgccgacca gttcgccaga tggcaacatc
      361 caggcagcgg cgtgagtaca accagtcagt tgccccactg gctgaaaatc gttgagatga
      421 cgtaatgcat catccagttt gttgagatca aacgtctggg tgaacggtag cggctgcacc
      481 ggttttccga tgtcattaag ttgctccacg ccgcatacgc cacagcccgt acgtcccgcc
      541 agcgcccggc ggcgctcctt caaccccata aagcggcggc tggaaagctc aatttgtact
      601 tcaagaccat tacaggaagg aacgacatcc atgccgaaga tatcgcgcgg actttctatt
      661 atcccctcgg aaagcgaaaa accgagcgca aagtactcaa ggtctttggg cgacgccatc
      721 atcaccacat gcgaaatgcc gttgtagacc agcgcaacgg gaacttcttc cgcgacctca
      781 tctaaccgtg ggtgttgtaa atcatcacgc cgccataact caatttgacg aacacctgtg
      841 atatttgtca cattttcgat ttcttttcgc tgtgtttttt tcactttctc ttaaccagat
      901 aagaacacac acaccaacat tatggtattc tgttacaaac ccttcctaga tggagggaaa
      961 ttgagccaat tctggacctt tgcggcccct tccgcaaaga aaaataactc ccactccctg
     1021 cacacgcagc aagcgaatgt aaatgggacg tgacaatgtc gaaacaagga gcaatccatg
     1081 caggtcagca gaaggcagtt ctttaagatc tgcgctggcg gtatggcagg caccacggca
     1141 gcggcactgg gttttgcacc cagcgtagca ctcgcggaaa cccggcagta taaactgctg
     1201 cgcacccgcg aaacccgtaa tacctgcacc tattgttccg taggctgtgg gctgttgatg
     1261 ttcagcctcg gtgacggagc aaaaaacgcc aaagcatcta tcttccatat cgaaggcgat
     1321 ccggatcacc cggtcaaccg cggtgcactt tgtccgaaag gcgctggcct ggtggatttc
     1381 atccactccg aaagccgtct gaagtttccg gaataccgtg cgccaggttc tgataaatgg
     1441 cagcaaatca gttgggaaga ggcgtttgat cgcatcgcca aactgatgaa agaagaccgc
     1501 gatgctaact acattgcgca aaacgccgaa ggcgtgactg ttaaccgctg gctctccacc
     1561 gggatgctgt gtgcttccgc gtcgagtaac gaaaccggct atttaacgca aaaattctcc
     1621 cgcgcgctgg gtatgctcgc ggtcgacaac caggcgcgtg tctgacacgg accaacggta
     1681 gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca accactgggt cgacatcaag
     1741 aacgccaacc tcgtcgtggt gatgggcggt aacgccgctg aagctcaccc ggtcgggttc
     1801 cgctgggcga tggaagccaa aattcacaac ggcgcgaagc tgattgtgat cgatcctcgc
     1861 tttacgcgta cggcggcggt agctgactac tatgccccta ttcgttccgg tactgacatt
     1921 gctttcctgt caggcgtatt gctgtacctg ctgaacaatg aaaaattcaa ccacgaatac
     1981 accgaagcct ataccaacgc cagcctgatc gtgcgtgagg attacggctt tgaagatggc
     2041 ctgttcaccg gctacgacgc ggaaaaacgc aagtacgata aatcctcctg gacttatgaa
     2101 ctggacgaaa acggcttcgc caaacgcgat accacgctgc aacatccgcg ctgcgtgtgg
     2161 aacttgctga aacagcacgt ttcccgctac acgccagatg tggttgaaaa catctgtggt
     2221 acgccaaaag acgcgttcct gaaagtctgc gaatacatcg cagaaaccag tgctcacgat
     2281 aaaactgcct cgttcctgta tgccctcggc tggacgcaac actccgttgg tgcgcaaaac
     2341 attcgtacga tggcgatgat ccagctgctg ctcggcaata tggggatggc cggcggcggc
     2401 gttaacgccc tgcgcggtca ctccaatatt caggggctga cggacctggg gctactgtcg
     2461 cagagtctgc caggttacat gacgctgcca agcgagaagc agaccgatct gcaaacctac
     2521 cttaccgcca acacgccaaa accgctgctg gaaggccagg taaactactg gggcaactac
     2581 ccgaaattct tcgtctctat gatgaaggcc ttctttggtg ataaagcgac ggcggaaaat
     2641 agctggggct ttgactggtt gccgaagtgg gataaaggct acgacgtcct gcaatacttc
     2701 gagatgatga aagagggcaa ggtcaatggc tatatctgcc agggctttaa ccctgttgcc
     2761 tcattcccga acaaaaacaa agtgatcggt tgtctgtcga aactgaagtt cctcgtcacc
     2821 atcgacccac tgaacactga aacctctaac ttctggcaga accacggtga gctgaacgaa
     2881 gttgactcgt cgaagatcca gaccgaagtg ttccgtctgc catcgacctg cttcgcagaa
     2941 gagaacggtt caatcgttaa ctcaggtcgc tggttgcagt ggcactggaa aggtgcggac
     3001 gccccgggga ttacgctgac tgacggtgag atcctctccg gtatcttcct gcgcttgcgc
     3061 aagatgtatg ccgaacaggg tggcgcgaac ccggatcagg tgctgaacat gacctggaac
     3121 tacgccattc cgcatgagcc gaagtcggaa gaagtagcga tggagagcaa cggtaaggcg
     3181 ctggccgata ttaccgatcc ggcaaccggt gcggttatcg tcaagaaagg ccaacaactt
     3241 agctcgttcg cccaattgcg tgatgacggt acaacctcct gtggctgctg gattttcgcc
     3301 ggtagctgga cgccggaagg caaccagatg gcgcgtcgtg ataacgccga tccgtctggc
     3361 ctcggtaaca cgctgggctg ggcatgggca tggccgctta accgccgcat tctgtataac
     3421 cgcgcctccg cagatccgca gggtaacccg tgggatccga agcgtcagtt gctgaaatgg
     3481 gacggcacta agtggaccgg ctgggatatt ccggactaca gcgcagcgcc tccgggtagc
     3541 ggcgtcgggc cgtttatcat gcagcaggaa ggcatggggc gtctgtttgc cctcgataag
     3601 atggcggaag gtccgttccc ggaacactac gagccgtttg aaacgccgct gggaactaac
     3661 ccgctgcatc caaacgttat ctcgaacccg gctgcacgta tctttaaaga cgacgccgaa
     3721 gcattgggta aagccgataa gttcccgtat gtcggaacca cctatcgtct gaccgagcac
     3781 ttccactact ggaccaaaca cgcgctgttg aacgcgattt tgcagccaga gcagtttgtg
     3841 gaaatcgggg agtcgctggc gaataaactt ggcattgccc agggcgatac cgtgaaagtc
     3901 tcctccaacc gtggctatat caaagccaaa gcggtggtga ccaaacgtat tcgcacgctg
     3961 aaagcgaacg gcaaagatat cgataccatc ggtattccga ttcactgggg ctatgaaggc
     4021 gtagctaaaa aaggctttat tgccaatact ttaacgccat tcgttggtga tgcgaacacg
     4081 cagacgccgg agtttaagtc cttccttgtg aatgtggaaa aggtgtaacg gagacgacat
     4141 atggcttatc aatctcaaga tatcattcgt cgttccgcga ctaacggtct gacccccgcg
     4201 cctcaggcgc gggacttcca ggaagaagtg gcgaaactca tcgacgttac cacctgtatc
     4261 ggctgtaaag cctgtcaggt ggcgtgttca gagtggaacg acattcgcga taccgtcggc
     4321 aataacattg gggtgtacga caaccccaat gatttaagcg ccaaatcgtg gacggtaatg
     4381 cgcttctcgg aagtggagca gaacgacaaa ctggaatggc tgatccgcaa agacggctgt
     4441 atgcactgtt ccgatccagg ctgcctgaaa gcgtgcccgg cggaaggtgc aattattcag
     4501 tatgccaacg gtatcgtcga cttccagtcc gagcagtgca ttggctgcgg ttattgcatt
     4561 gcgggctgtc cgttcgacat tccgcgcctc aacccggaag acaaccgcgt ctacaaatgt
     4621 acgctgtgcg ttgaccgcgt ggtggttggg caagaaccag cctgcgtgaa gacctgccca
     4681 acgggcgcga ttcacttcgg tacgaaagag tcgatgaaaa cgctggcgag cgagcgcgtt
     4741 gctgagctga aaacccgcgg ttacgacaat gcgggtctgt acgatccggc aggcgtcggt
     4801 ggtacacacg tcatgtacgt gctgcaccat gctgacaagc caaatctgta tcatggcttg
     4861 ccggagaacc cggaaatcag cgaaaccgtg aaattctgga aaggcatctg gaaaccgctc
     4921 gcggctgttg gctttgcggc tacctttgcg gccagtatct tccactacgt gggtgtcggt
     4981 ccgaaccgtg cggatgagga agagaataat ctgcacgaag agaaagacga ggagcgcaaa
     5041 tgaaacgacg tgacaccatc gtgcgctaca ccgcgccgga acgtatcaac cactggatca
     5101 ccgccttctg cttcatcctg gcggcggtga gcgggctggg ctttttgttc ccgtccttca
     5161 actggttgat gcaaatcatg ggcacaccgc agctggcgcg aattctgcac ccgtttgtcg
     5221 gcgtggttat gtttgcctcg ttcatcatca tgtttttccg ttactggcat caaaacctaa
     5281 tcaatcggga tgatatcttt tgggcgaaga atattcgtaa gatcgtcgtc aacgaggaag
     5341 taggtgacac cgggcgttat aacttcggtc agaaatgcgt tttctgggcg gcgattattt
     5401 tcctggttct gctgctggtg agcggcgtga ttatctggcg tccttatttt gcgcctgctt
     5461 tctcaatccc ggtgatccga ttcgcgttaa tgctgcattc atttgccgca gtggcgttaa
     5521 ttgtggttat catggtgcat atctacgccg ccctttgggt gaaaggcacg attaccgcca
     5581 tggtggaagg atgggttacc agcgcctggg cgaagaaaca tcacccgcgc tggtaccgtg
     5641 aggtccgcaa gacaacggaa aagaaagctg aatgagtatt cgcataatcc cgcaagatga
     5701 gctgggttcg agcgagaaac gtacggcgaa tatgattccg ccgttattgt tccctcggct
     5761 caagaattta tacaaccgcc gcgccgagcg tctgcgcgag ctggcagaaa ataatccgct
     5821 gggtgattac ctgcgctttg ctgcgcttat cgcccacgcc caggaagtgg tgctgtacga
     5881 ccatccgctg gagatggatc tgaccgcgcg cattaaagaa gccagcgcac agggcaagcc
     5941 cccgctggat attcacgttc tgccgcgtga taagcactgg caaaagctgc tgatggcgct
     6001 aattgctgag ctgaaacctg aaatgagcgg cccggcgctg gcagtgattg agaatctgga
     6061 gaaggcatcg actcaggagc tggaagatat ggccagcgcg ctgtttgcct ctgatttctc
     6121 gtccgtcagc agcgataaag cgccgtttat ctgggctgcg ctgtcgctct actgggcgca
     6181 gatggccaat ctgatccccg gcaaagcccg cgctgaatac ggcgaacaac gtcaatattg
     6241 cccggtatgt ggctctatgc cggtgtccag catggtgcaa attggcacca ctcagggtct
     6301 gcgttacctg cactgcaacc tgtgtgaaac cgaatggcac gtagtgcgcg taaaatgcag
     6361 caactgcgag cagagcggca aactgcatta ctggtcgctg gatgacgaac aggccgcgat
     6421 taaagccgaa agctgcgatg actgcggcac ttacctgaag attctttatc aggaaaaaga
     6481 accgaaagtt gaagccgtgg cagatgacct cgcctctctg gtactggacg cgcgaatgga
     6541 gcaagaaggc tacgcccgta gttccattaa cccgttcctg tttccgggtg aaggagagta
     6601 atctctgacg ttttccgggt ggcgcaggtc gcccggatta cagctcatag ggtgtgacga
     6661 caccatctct cgtattccgc gttacccatt caagatcatg ccgcaattgt ctgtttgcct
     6721 cgttcttccc tccaacacat cgataaggtt atgggtatgt cactgatgac aagcgtatga
     6781 ttgcctgatg cgctgcgctt atcaggccta ccaataaact tcaatatatt gaatttgcat
     6841 gactttgtag gccggataag gcgttcacgc cgcatccggc atgaacaacg cgcacgttgt
     6901 cagcaatctg attgacatca ttttttccca ttaccattcc tcgcgcagct tacgctgata
     6961 ttcgacacca tcttcctcac acccttgcca gatgccagga acactggttc ttgctgtttg
     7021 cgattgattt atcaggtatt gatctaccgc ttcccttaat aggtccgcac gaggaagatt
     7081 acgctgcacc tcgagatcat caagttgctt aatcacctca ttcgataaat cgagtaaaat
     7141 tctgcccata tctatacctg ccagagagtt cataaatatc tcgccaatat catttttgaa
     7201 tctatggaga gaaaagtacc cttgccgaat ctttaaagaa agctcattta cgcatcattt
     7261 tttatttttg cgaggcgttt tccagatacg atgtaacagt tgcagttatt ataagttcaa
     7321 gtaaaacagc cttgtattaa taccagaata agggcatctg ttttttatat tcaagaatga
     7381 aaaatttttt tcattcctta tgctccttac actgatcatc aacaccaact acctccccct
     7441 tttgcctgcc tccctttgct ttacgcaccg gaaattgctg ccgctccaga tattgctcaa
     7501 ccgcttcgcg taaaattgca gatcgtgaaa ccttacgcca gccccgaagc ttgttcagcc
     7561 gtttaattgc ctcttctgat aaatgaagaa aaaccgtatt catcgccatt accaccacct
     7621 tacatatatt actcattaat gtatgtgcga aaattacgcc gaaaaagcat ttaaaaaaat
     7681 gcggattttt gcatgtcatt ttgtttttgc gaggcgtttt ccagaaataa taagagcgtt
     7741 gcagttgttt catttattac tcaaaaatgg tttagttatc gcccaggatg agttatgggt
     7801 gctatgaata tattatattc acggatattt atttgcataa tctgaatggt gtagccgaaa
     7861 aacgtcagca tcctaacagc accaggattt aaggtgaaat taatctttca tcacagaccg
     7921 gtcagtactg ccaccaaccg gtctttactt gctcatcgtt ataattttgt tggtaacagt
     7981 tcgggaaaca aggaattcct tccggaacac ccccggcatc atccgatagc attgacgtca
     8041 atcatcagcg cgacgatgag tttttttaat cgcatcatag ggtggcgacg ttcagcacga
     8101 ttacgggtca tagcaaaact cctgagcagg cggccactat gccgctttca ctggagttct
     8161 gaatactggt actttctgta tggcttcgcc atgcccctcg cggctaagta cctgcacaca
     8221 tctctctttg tatgtcattt ttgcaatgca ttgtccgaat tctgataatc ctaatgagtt
     8281 ttactcgtca gactcccatc cctacggtat acgagttata ttcatgaatg aaatcgacat
     8341 ttttaaaaaa cgaatcttgt aaaacatcag tgttgatatt accttgcaat ccaacattga
     8401 atttgttaga gacatgaaga actcgaatat caaaaaggtt ttttaacatc attaattctt
     8461 cgggtgttaa tgaatcgcaa ctaaaatatc cctgttgatc aaaaagattt tctatttctc
     8521 cactatcggc gaggcgtttt tcaaggagtg tttgcaacat cgagttgcca aggacaatgg
     8581 atatcgtgcg atctttcgca gatttacacc ccatcacaac ggcaacattc gtatccccct
     8641 caagagtttc atttatcgcc gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat
     8701 ttctttcgtg atttaattta tctaccagtt cattccattt atcaaaagcc gggctatatt
     8761 tgttgcgcac agaaatatcc gactggttaa acaattcagc catgcgattt tttatcatct
     8821 cgatatccgg cgaaccagaa agactttgcg cataatacgg tcctgatgat gttgcataca
     8881 ttaatgccaa gcccacaact gtcgccatga tgggcgcagc cacggttttg gcaagggcga
     8941 cgctgcgatg gctatgcgct tcacaggcta aaggaacata ttccactgac tgcggtaatt
     9001 ccttacatgc ctccctcatc attgcggtat gctttacaaa caacggtttt tcactgccca
     9061 aaaaaacttt ctgcttaatt gcactaagaa aacgcaaatc aaaaataatt gtttttggtt
     9121 tattattttt tggcacattt cctattatcc cgctaagaac cttccttgct gcatggagat
     9181 ttttttgcac agcacataac acaattgatg aagccttctc cactgcatta ttgattttaa
     9241 ttttaaaatc cgcatgatat acgttgacgg gcaatgcaag tatatctgga cctacatctt
     9301 ttgctgagta tttattggtg atcgacggac gtgaacccac gagaaaactt agctgcaaat
     9361 tatgctctat attatcagct gtttttgtgg tgtggttaaa catagagatg ccacctttca
     9421 ttctttcaca catatcctca actttttcac ttcgttcttg tggcaatttg gctttaacat
     9481 taatttgatg atttttaccc gtgcattcct taactacaag gggaatgtta tctaaagtgc
     9541 catacaggct aataacgtca ccaggatttt tatcatgtaa gtaagtttga ttaatacttg
     9601 aagcattcac aaagagggtc aatattataa ctgtttataa cttcacctac atcaagtgtg
     9661 tagtgcttac tctggagact gccatcctta tttaattgta tatcaatcaa tatatccccg
     9721 gactttgtca ccatatcttt gccataaacc atttgcatag ccatttcgag gagattttta
     9781 tctaactgat tgcgggcgtt gagtaagcga ttaccaaata atttatatac tgttcccgtc
     9841 attgccagcg ctgcgctttc tggtactttg cgtttccaca gtgaggtttc tctattttta
     9901 aacaggtcat attcattatt actcgacaaa ccatgcttca gttcgttaac acaatcgcac
     9961 agttgctcct caccttccat tttaaggcaa agtgaaaatt gatttaatgg cgtttacttc
    10021 ggtgatttat tactcattag ttgtttttct aaattaacaa cagaataact ttcttgttta
    10081 atgagattaa tattggcaac cattatagcc tccttcccac ggtttaagag caaacataca
    10141 acataatgat tcataacata cagagcagta aatgaatgat actgacaatg tatgccagcc
    10201 cattatcttt gcataatgtt tgtgagctat agattttaaa aatatttatg aaaaaagtat
    10261 ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac ctgaactaag atgatgagtt
    10321 aagcattatc tgcaataatt gccactcagc tctacaaata atatttaaat gctcctgaac
    10381 agtgatgtag caagacacgt atggtgccaa cgaagaataa aaatttataa agttcttatt
    10441 caatataacc ggaatagtca gggactgatg taaatgaccg gaatgaatga gagaggcagc
    10501 accgcccctc tccataagct actcttcttc gttcccgccc tcttcatacg ggccaaatgg
    10561 cttcgcgggc agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa
    10621 gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc
    10681 gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg ctaatcggtt tgctgtagcg
    10741 gatatgcgca tccgccagaa taatcgttcc gccgaggtgg cgttcacgca gcatcagcca
    10801 gataagcccc caaccggtga gcgtcgccag cgagaataaa ctcccggcaa acagcgtatg
    10861 gtgcggattc tgattgccgg tttccggcat ggtggtgata aatttttgcc cggtatattg
    10921 ttgaatacgc acgcccattt tttcactaag cgggatatgt tcgtaccacg cctgttgcag
    10981 ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga gtggcgacgg gcttaatcat
    11041 caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt tctccctgat taacaaaccc
    11101 cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc
    11161 ttcctgacgc gccaccgact ccagggtcat cgccatcagc gttcctaacc ctttgtcctg
    11221 cacgtccggg tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacaa
    11281 tcggcctacc gccaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc
    11341 atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca
    11401 gcgaaactga tagtaacgct ctaactcttc ttctgtttgt ggaacctgaa ggtgatacat
    11461 agcgatactc tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag
    11521 aatgtcacgg ggccatcatt gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt
    11581 tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat agtcatataa cgcctctgcg
    11641 cgatccggtg atgcgccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggcg
    11701 agggtaaact gggaaaccac cagtacactg ccgcccgcct gttgcacgtt gagattcatc
    11761 ttgccttcgg catcgctaaa gatgcggtag ccgagcacac gctcgcacag acggtttgct
    11821 ttctgttcgt cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttcg
    11881 cccgtcactt ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc
    11941 atggttcgtc gtcttcttct tgttcagctg cttgttttag tttgcggtat tccccgagag
    12001 tgacagtaat ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga
    12061 gaatggggat caccgccagc acaccgtaaa tgagctgata tgacgggaac atggtgatat
    12121 aaagcgcgaa acctttcttt cctgcttcga acaggagtgc ggcgacaaac gcgccgacaa
    12181 tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct gtacagcaac cagaaggaga
    12241 tccacgacaa cagcaacgga aaaatacgca gcacgttgtc gatgacagta ttgagatcgc
    12301 tcgcccagcg cagggagagc aaataggaac tgatcgccag actggcccct gccagcagcg
    12361 gccccagcgt taaaatcatc cagtacacgg cgaacgagta aatttttggt cgcgctcgtt
    12421 tactgcgcca gatggtattc aacgcgctat cgatggagta catcaacaat aacgcggtga
    12481 cgatcagccc gcacgcccca acggcggtca tcttgttgga attagcaaca aattgttcga
    12541 tataccgctg aataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta
    12601 actgaatgct gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa
    12661 ccagcggcac taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt
    12721 tgtcctcatc aatacgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacggt
    12781 gcctggcttt gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt
    12841 ggttttatct ttcaccagaa tactggtaat gcctagctga ttagctcctt ctatattatc
    12901 ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg
    12961 ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct tgcgacagat agatatggtc
    13021 agcagcatca cgaatttccg ggtattcttc cggccagaag gtggtatgca ggcggttggt
    13081 attggaaagc accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac
    13141 ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag
    13201 cggtagagcc atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg
    13261 ctcatgctga tgaaacgcct cccccatatg aaaactcttc ttaagcgatg ccagcggaat
    13321 acgcgttaaa tcgctccagg ctcccagcac acggttaaag tcgatatcga caatcacatt
    13381 acctaaatca aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta
    13441 gcgggaaagg atgggtttgg ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa
    13501 atctacgcgt cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt
    13561 agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt
    13621 tcaaccgccg ccgaagcatg atacttagtg tgatcggcga ccagcagaat atttctcgcg
    13681 tgcgccatca tcgttttcac cacgttagct tcgtttacat caaactccat caacgcgcca
    13741 tcgctctcaa tcgccccaac gcttgttacc agataatcag cacgaaaatc agccacaaag
    13801 gacgccgctg aagggccaat gatcccgcta ttatgagagc gcaacgtacc gccgggcacc
    13861 atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt
    13921 atccgcaaat gattatggtt aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg
    13981 gtgataaata ttgttgaacc atcaggaata tagtctgcca ccgcttcggc aatcgctttt
    14041 ttttctcggt ttgcgaaacc tcacgctgct cgaacgccgt attaacgacg ctggaagccc
    14101 gacccgcgcc accgtgatgg cgcgtaatca ggccttgctc gcttaattta cgaatatccc
    14161 ggcggaccgt ctgcgtggag acatccagca gatttgccag ctcatcaata ttcatatacc
    14221 cacgctcggc gatcagcatg aggagttggt cgtgccgcgg gttaccggtt agttcggtaa
    14281 ggctcatgaa aattcctcga aaaaccatca ccctggcatt ttatacaaaa agtgacaaaa
    14341 aagatcgggt ttgatcacag tcagggatcc cggctcgtcc accgggacgt gtgcatttta
    14401 acgccgctac accgctggcg aagcggactg actccgctaa atccccactt gttgccagcg
    14461 ccaccgccaa agcgccgtga aaaacatcac ccgcacctgt ggtatctacc acatcaactt
    14521 tgaaggccgg ttgatgctga cgcccaccat tttccagcca gtcgcagcct gcgctaccct
    14581 gggtcacata gacatgacca tttgtgagcg tttgtgcctg ttttagcgca ctggccatct
    14641 ctttcacgcc cgttaagcgc gccagacccg gttctgaaaa ggccgcgtga tcgcttaatg
    14701 ccaccagctc actgatatcc tgcggcgtaa tgtccccgtc cagaacggtc atcacacccg
    14761 cctgacgggc cagggtgaag gcttttttaa cgccgtcgtg ccagcgtaca tctgccagca
    14821 caacatccca ctgagagaaa tcaatttcct ccaaccactc tgcgtcaggc agcagatccg
    14881 ggctggggta gttaatgatt atccgctcgc ctttggtatc caccatgatg gcggattgcg
    14941 aagatttcgc ctggttatac cgtttggtgt aacgggtgtt aacgccccag gattccagct
    15001 ctgctagcag gctgttgccc gcgtcgtcat cacctacgcg accaataaaa tcgacctgcg
    15061 cccccagcct tgccgccgca accgctgccg tcgccgctgg cccgccacca acttccgtat
    15121 aatttctcgc cacgtattta ccgctctccg tcggtaaccc ttccacgtaa tagatgcgat
    15181 ccatcacggt tatacctaca caagcaacac gaatcatggt cattccttaa acattttggg
    15241 atgcgttcat tttcatttca ccaattgttt aaaaagtgac ggctgtcaat tttttgacga
    15301 taaattacaa atgcgatcaa aaacagacaa gcaaagcatg tcataaatga caaaaagtga
    15361 catcactgta ttcaggagag ggttatggca gcaatcgcgt ttatcggttt aggacaaatg
    15421 ggttcgccaa tggcgagcaa tttattgcag caagggcatc aacttcgcgt ctttgatgtg
    15481 aacgccgaag ctgtgcggca tctggtagac aaaggtgcga ctcccgccgc caacccggtg
    15541 caggccgcta aagatgccga atttatcatt accatgttgc cgaatggcga tctggtgcgc
    15601 aacgtgttgt tcggtgaaaa cggcgtttgc gaaggcttat ctaccgatgc gctggtcatt
    15661 gatatgtcca ccatccatcc gctgcaaacc gataaattga ttgccgatat gcaagccaaa
    15721 ggcttcagca tgatggatgt tccggtaggc cgtacttctg caaatgccat taccggtact
    15781 ctgttactgc tggctggcgg caccgctgaa caagttgaac gtgccacacc gatcctgatg
    15841 gcgatgggca gtgagttgat caacgctggc ggtccgggca tggggatccg cgttaagctc
    15901 atcaacaact acatgagcat cgcgctcaat gcgctttcgg cagaagccgc cgttttgtgc
    15961 gaagccctga atcttccctt cgatgttgcc gtcaaagtga tgagcggtac cgccgccggt
    16021 aaaggtcact tcaccacttc ctggccaaac aaagtcctca gcggagatct ttctcccgcc
    16081 ttcatgatcg atcttgccca taaggatctt ggcatcgccc ttgatgtcgc caaccagctg
    16141 catgtgccaa tgccgctggg ggccgcctca cgggaggttt atagccaggc gcgcgcatcg
    16201 ggcggttttg ccatgctggc ggtcgatcag cgcgaagcca tgcgcatgat gtttgccgcg
    16261 gctggggcac ccgctccggt agccgatagc gttttaactg atttcaaagt taacgctgca
    16321 aaggccctct cgccttatgc ctcggcgatt ctggtagatc aacaattctg ctaccgccag
    16381 gtggttgagc aaaacgcgat tgccaaaagt tgcgccatga ttgtcgccgc cgatgagttc
    16441 attcctggca acggtattcc ggtcgatagc gtggttattg accgcaaaat caatccgcta
    16501 cagatcaaac aagacggtgg caaagcctta aaactgctgg tgctgtggcg tagtgatgaa
    16561 gatgcgcagc aacgtctgga tatggtgaaa gagttcaacg gactgtgcca ctcacacggt
    16621 ctggtaagca tcattgagcc agtcgtccgc ccaccgcgtc gtggcgataa attcgatcgc
    16681 gaacaagcgg tcatcgatgc cgccaaagag ctgggcgaca gtggcgctga cctctacaaa
    16741 gttgaaatgc cgctttatgg caaaggtccg caacaagagc ttctctgtgc ttcacaacgt
    16801 ctgaatgacc atatcaatat gccatgggtg atcctctctt ccagtgtcga cgaaaaactg
    16861 ttcccgcgtg ccgtacgcgt ggcaatgacg gcgggcgcat cgggattcct ggcaggccgt
    16921 gcagtctggg catcggtcgt cggtttacca gacaacgagc tgatgctccg tgacgtttgc
    16981 gcaccgaaat tacaacaact tggcgatatc gtcgacgaaa tgatggctaa acgccgctaa
    17041 gatgaggata aaaggaatga aatggtttaa caccctaagc cacaaccgtt ggctggaaca
    17101 ggaaaccgac cgcatctttg attttggtaa aaattccgta gtgccgactg gttttggctg
    17161 gttaggcaat aaagggcaaa tcaaagaaga gatgggcacc catctgtgga tcacagctcg
    17221 tatgttgcac gtttattccg ttgctgcggc gatgggtcga cctggcgctt acgcgttggt
    17281 tgatcacggt attaaagcca tgaacggcgc actgcgtgat aaaaaatatg gcggctggta
    17341 tgcctgcgtg aatgacgagg gcgtggtgga tgcctccaaa cagggctatc aacacttctt
    17401 tgctctgctg ggtgccgcca gcgccgtcac aacgggtcac ccggaagcgc gcaagctgct
    17461 cgattacacc attgagatta tcgagaaata tttctggagc gaagaagagc agatgtgcct
    17521 ggaatcctgg gacgaagcct tcagcaaaac cgaagagtac cgcggcggca atgccaatat
    17581 gcacgcggtg gaagctttct tgattgttta tgacgtcact cacgacaaaa aatggctgga
    17641 tcgcgcgatt cgcgtggctt ccgtgattat ccacgacgtc gccagaaata atcattatcg
    17701 cgttaacgaa cacttcgata cccagtggga tccgctgccg gattacaaca aagataaccc
    17761 ggcgcatcgc ttccgcgcgt tcggaggtac accaggccac tggatcgaat ggggccgttt
    17821 aatgctgcac atccatgctg ccctggaagc ccgttgcgaa caaccaccag catggctgct
    17881 agaagatgcc aaaggtctgt ttaacgccac cgtgcgcgat gcctgggcac ccgatggcgc
    17941 ggacgggatt gtttataccg ttgactggga aggaaaaccg gtggtccgcg aacgtgtacg
    18001 ttggcctatc gtcgaagcaa tgggtacggc ctacgcgctc tacaccgtca ctggcgatcg
    18061 ccagtatgaa acctggtatc aaacatggtg ggagtactgc attaagtacc tgatggacta
    18121 tgaaaatggt tcctggtggc aggagctgga tgcggacaat aaggtcatca ccaaagtctg
    18181 ggacggcaaa caggatattt atcacctgct gcattgcctg gtgatcccgc gtatcccgtt
    18241 agcgcccggc atggctccag cggttgcagc gggtctgctg gatattaatg cgaaataact
    18301 gatctgaaaa tagggtgcgg gttttcccct caccctaacc ctctccccaa aggggcgagg
    18361 ggaccgtccg gtgcagtttt tgggtttcac atcagcctca cgggaggctg tatgtcgtta
    18421 attaaggtgc cttgtatgca aatcaccaat atgcattgca gtggacagac cgtttcactt
    18481 gccgcggggg attatcacgc gaccattgtg accgtcggtg ctggtctggc tgaattaacc
    18541 tttcagggtt gtcatttagt catcccgcac aaacccgaag agatgccgct ggcgcatctg
    18601 ggtaaagtgc ttattccgtg gccgaaccgg atcgccaacg gctgttaccg ttatcagggg
    18661 caggaatatc agttacccat taatgaacat agttcaaaag ccgctattca cgggctgctt
    18721 gcctggcgag actggcaaat tagtgaatta accaccacca gcgttaccct gacagctttt
    18781 ctgccgccca gttacggcta tccgtttatg ctggcttcac aagttgttta ttcgctcaac
    18841 gcacacacgg gtctttccgt tgagatcgca agccagaata tcggcactgt cgcagcgcct
    18901 tatggcgtgg gtattcatcc ttatttaacc tgcaacttaa catctgttga tgaatatctg
    18961 ttccagcttc cggcaaacca ggtttatgcc gtcgatgaac acgtaaatcc gacaacgctt
    19021 caccacgttg atgagctgga tttaaatttc acgcaggcga aaaaaatagc cgcgacaaaa
    19081 atcgaccaca cctttaaaac cgctaatgat ttgtgggaaa tgaccattac ccatccacag
    19141 caggcgcttt cggtaagcct ttgttccgat caaccctggg tgcaggttta tagcggtgaa
    19201 aaattacagc gtcagggact ggcagttgaa cctatgagtt gcccgcctaa cgcctttaat
    19261 tcaggtatcg acttattatt actggaacca ggaaaaccgc accgcctgtt ttttaatatt
    19321 tacggacaga gaaaataact tgtattgatt agcagtcaaa atcattgaag aacatttcac
    19381 tcgctaaagg caacattatg tatcttcaat ttattaagtt aaaaacagct cagcttctgt
    19441 aaagttttta tatatcagtt ttatgagaat cattttacat aagcctgtaa taacaggaag
    19501 gcaacaatct cgttctcaac tctatggaga ttagttatgg atacgccacg tccacagtta
    19561 ttagattttc aatttcatca gaataacgac agttttaccc tacattttca acaacgtctt
    19621 attttaaccc atagcaaaga taatccttgt ttatggattg gctcaggtat agcggatatc
    19681 gatatgttcc gcggtaattt cagcattaaa gataaactac aggagaaaat tgcgcttacc
    19741 gacgccatcg tcagccagtc accggatggt tggttaattc atttcagccg tggttctgac
    19801 attagcgcca cgctgaatat ctctgccgac gatcagggcc gtttattgct ggaactacaa
    19861 aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc agaggaccat
    19921 atctacggct gcggcgaaca gttttcctac ttcgatctgc gcggcaaacc gttcccgcta
    19981 tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg gcaggccgac
    20041 tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc tacgtttgtc
    20101 agcacgcaga agtattactg ccatgttgat aacagttgct acatgaactt cgactttagc
    20161 gccccggaat accatgaact ggcgctgtgg gaagacaaag ctacgctacg ttttgaatgt
    20221 gctgacacat acatctccct gctggaaaaa ttaactgccc tgctgggacg ccagccagaa
    20281 ctgcccgact ggatttatga cggggtaacg ctcggcattc atggcgggac ggaagtgtgc
    20341 cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg ttaacggcat ctgggcgcag
    20401 gactggtctg ggatccgtat aacctctttt ggcaagcgcg tgatgtggaa ctggaagtgg
    20461 aacagcgaaa actacccgca actggattca cgcattaagc agtggaatca ggagggcgtg
    20521 cagttcctgg cctatatcaa cccgtatgtt gccagcgata aagatctctg cgaagaagcg
    20581 gcacaacacg gctatctagc aaaagatgcc tctggcggtg actatctggt ggagtttggc
    20641 gagttttacg gcggcgttgt cgatctcact aatccagaag cctacgcctg gttcaaggaa
    20701 gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga cttcggcgag
    20761 tatctgccca ccgacacgta cctgcataac ggcgtcagtg ccgaaattat gcataacgcc
    20821 tagcctgcgc tgtgggcgaa gtgtaactac gaagcccttg aagaaacagg caagctcggc
    20881 gagatcctct tctttatgcg cgccggttct accggtagcc agaaatactc caccatgatg
    20941 tgggcgggcg accagaacgt ggactggagt ctcgacgatg gcctggcgtc cgtagtcccg
    21001 gcggcgctgt cgctggcaat gaccggacat ggtctgcatc acagcgacat tggcggttac
    21061 accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg cgatttcagc
    21121 gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa ctggcagttt
    21181 gacggcgatg cggaaactat cgcccatttt gcccgtatga ctaccgtttt caccaccctg
    21241 aaaccttatc tgaaagaggc cgtcgcgctg aatgcgaagt ccggtctgcc ggtcatgcgc
    21301 ccgctgttcc tgcattacga agacgatgca catacctatt ccctgaaata tcagtacctg
    21361 ttaggccgcg acattctggt cgctccggta cacgaagaag gtcgcagcga ctggacgctc
    21421 tatctgccgg aggataactg ggtacatgcc tggacgggtg aagcgttccg gggcggggaa
    21481 gttaccgtta atgcgcccat cggcaagccg ccggtctttt atcgcgccga tagcgaatgg
    21541 gcggcactgt tcgcgtcgtt aaacagcatc taatcacatc cgcccgtggg gaaactcatg
    21601 ggcaaaggga gaacatcgat gagtcacatc acaacggaag atccagcaac tttacgcctg
    21661 ccctttaaag agaaactctc ttacggtatt ggcgacctgg cctctaacat cctgctggat
    21721 atcggtacgc tttatctttt gaagttttat accgacgttc tggggctgcc aggcacctat
    21781 ggcggcatta tctttttgat ttcaaaattc tttactgcgt ttaccgatat gggtaccggc
    21841 attatgttgg attcccgacg caagatcggt ccaaaaggta agttccgtcc ttttattctg
    21901 tatgcgtcat tcccggtcac cttactggcg atcgccaact ttgtcggcac accgtttgat
    21961 gtcaccggta aaacggtgat ggccactatt ctgtttatgc tttacggact gtttttcagc
    22021 atgatgaact gctcctacgg cgcaatggtc ccggcaatca ccaaaaaccc caacgaacgc
    22081 gcctcactgg cggcatggcg tcagggcggc gctacgctgg gcctgctgct gtgcacggtg
    22141 ggattcgtgc cagttatgaa tcttatcgaa ggtaatcaga aacttggcta tatcttcgcc
    22201 gccacgctgt tttcactgtt cggcctgctg tttatgtgga tctgctactc aggcgtgaaa
    22261 gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa agcctggcct gttgcaatct
    22321 ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt gtattgccaa cctctgcacc
    22381 ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt acacccagta cgtacttaac
    22441 gatcccatcc tgttgtcgta tatgggattt ttcagcatgg gctgtatttt catcggcgtg
    22501 ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga aggtctatat cggcggcctg
    22561 ctgatttggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc ggtcagcttt
    22621 gtggcgttct cctgcctggc gttcttcggc tcagcgtttg ttaacagcct gaactgggcg
    22681 ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gcgtgcgttc ggaaggcacg
    22741 gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg cgctggctgg tttcttcccc
    22801 ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg cacaggctga ccacaccatt
    22861 gaagggttac gccagttgat cttcatctac ccaagcgcac tggcggtagt caccattgtg
    22921 gcgatgggtt gcttctacag cctgaacgag aagatgtatg tccgcattgt ggaagagata
    22981 gaagcccgta aacgcacggc gtaataataa taaacgacgc cctgcggggc gttataagga
    23041 gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga aaaaatcgcc
    23101 tatggtatgg gcgacgtcgg ttcgaattta atgctctgca tcggtactct gtatctcctc
    23161 aaactttata ccgatgaatt agggatgcct gcttactatg gcgggattat ctttctggtc
    23221 gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat ttttactcga ctcgcggaaa
    23281 aatattgggc ctaaaggcaa atttcgtccc tttattttat atgcagcagt tccggcggcg
    23341 ttaattgcaa cgctccagtt tatcgccacc accttttgtt tgccggttaa aacgacgatt
    23401 gccaccgcgc tgtttatgat gtttggactt tcatacagtc tgatgaactg ctcgtatggt
    23461 gcgatgatcc cggcaattac caaaaacccg aatgagcgcg cgcagctcgc ggcttaccgc
    23521 cagggtggtg caaccatagg gctattgatt tgtaccgtgg cgtttattcc gttgcagtcg
    23581 cttttttctg actcaaccgt cggttatgcc tgtgcggcac ttatgttctc cattggcggc
    23641 tttattttta tgatgctgtg ctacagaggc gtcaaagagc attatgtgga cacagcgcca
    23701 accggacata aagccagtat tctcaaatct ttttgcgcga tatttcgtaa tccaccattg
    23761 ctggttttat gcattgctaa cctgtgtact ctggcggcat ttaatatcaa actggcgatt
    23821 caggtctatt acacccagta tgtgctgaat gatattaatt tattgtcgtg gatgggattc
    23881 ttcagtatgg gatgcatcct cgtcggcgta ttactggtgc cagtaactgt aaaatgtttt
    23941 ggtaaaaaac aggtttattt agctggcatg gtgctatggg cggtgggtga tatactgaat
    24001 tatttctggg gaagtaactc tttcactttc gtcatgttct cttgtgtcgc cttttttggc
    24061 acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga ttacggtgaa
    24121 tggaaaaccg gtattcgtgc cgaaggttct gtttataccg gttatacctt ctttcgtaaa
    24181 atttctgccg cacttgctgg cttcttgcca ggcattatgc tgacgcaaat tggttatgtt
    24241 cccaacatcg cgcaaagcga tgcgacatta cagggtttgc gtcagctcat ttttatctgg
    24301 ccttgtgcac tggcaattat tgctgcattg acgatgggat tcttttacac actcaatgaa
    24361 aaacggtttg cattgattat tgaggaaatc aaccaacgaa aaaataaaga gatggcgaca
    24421 gaaaaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc catcctggtg
    24481 ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaagatta atgcaataat
    24541 tttattatct tctctgacct ctgcctcggt atttgctggc gcatacgtcg aaaacaggga
    24601 ggcatataat cttgcctccg atcagggcga ggttatgcta cgtgtgggtt ataacttcga
    24661 tatgggcgcg ggtattatgt taaccaatac ctacaccttt cagcgagaag atgaactaaa
    24721 acatggctat aatgaaattg aaggttggta tcctttattc aaaccaaccg ataaattaac
    24781 catccagccc ggtggctt
//
LOCUS       DADLTF010000051        24460 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.051, whole genome shotgun sequence.
ACCESSION   DADLTF010000051 DADLTF010000000
VERSION     DADLTF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.051"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..487)
                     /locus_tag="JN749_001939"
     CDS             complement(<1..487)
                     /locus_tag="JN749_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="required for cellulose biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HAY7368370.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LL"
     gene            760..948
                     /locus_tag="JN749_001940"
     CDS             760..948
                     /locus_tag="JN749_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368371.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            960..1670
                     /gene="bcsQ"
                     /locus_tag="JN749_001941"
     CDS             960..1670
                     /gene="bcsQ"
                     /locus_tag="JN749_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HAY7368372.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1667..4280
                     /gene="bcsA"
                     /locus_tag="JN749_001942"
                     /pseudo
     CDS             1667..4280
                     /gene="bcsA"
                     /locus_tag="JN749_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4291..6630
                     /gene="bcsB"
                     /locus_tag="JN749_001943"
     CDS             4291..6630
                     /gene="bcsB"
                     /locus_tag="JN749_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HAY7368373.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6637..7743
                     /gene="bcsZ"
                     /locus_tag="JN749_001944"
     CDS             6637..7743
                     /gene="bcsZ"
                     /locus_tag="JN749_001944"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HAY7368374.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7802..7978
                     /locus_tag="JN749_001945"
                     /pseudo
     CDS             7802..7978
                     /locus_tag="JN749_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            8306..10054
                     /gene="bcsC"
                     /locus_tag="JN749_001946"
                     /pseudo
     CDS             8306..10054
                     /gene="bcsC"
                     /locus_tag="JN749_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138097.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10136..12124
                     /gene="hmsP"
                     /locus_tag="JN749_001947"
     CDS             10136..12124
                     /gene="hmsP"
                     /locus_tag="JN749_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HAY7368375.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12308..13594
                     /locus_tag="JN749_001948"
     CDS             12308..13594
                     /locus_tag="JN749_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HAY7368376.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFSSGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13815..15311
                     /locus_tag="JN749_001949"
     CDS             13815..15311
                     /locus_tag="JN749_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7368377.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15407..16555)
                     /locus_tag="JN749_001950"
     CDS             complement(15407..16555)
                     /locus_tag="JN749_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7368378.1"
                     /translation="MFKSDAARTRQRLSARKGRTLPPGRGKFPHSTTESFRNTFWLKK
                     IMEHCFNMVDQQTTTAQTNANFLQIRFTTMSKKIAVIGECMIELSEKDADVKRGFGGD
                     TLNTSVYIARQVDPAALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGL
                     YYIETDSTGERTFYYWRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTS
                     REKLLSLLRECRAKGGKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDA
                     LWGQQPVEDVIARTHNAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTA
                     AGDSFSAGYLAVRLTGGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16568..17335
                     /gene="pdeH"
                     /locus_tag="JN749_001951"
     CDS             16568..17335
                     /gene="pdeH"
                     /locus_tag="JN749_001951"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HAY7368379.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17405..19465
                     /locus_tag="JN749_001952"
     CDS             17405..19465
                     /locus_tag="JN749_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HAY7368380.1"
                     /translation="MSKAGKITAAISGVFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19647..20969)
                     /locus_tag="JN749_001953"
     CDS             complement(19647..20969)
                     /locus_tag="JN749_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HAY7368381.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="JN749_001954"
     CDS             complement(21380..22393)
                     /gene="yhjD"
                     /locus_tag="JN749_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HAY7368382.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22442..23413)
                     /locus_tag="JN749_001955"
     CDS             complement(22442..23413)
                     /locus_tag="JN749_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7368383.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKNLLLPRLSEFLYQHPGIELELSSHNRPVDILH
                     DGFDCVIRTGALPEDGVIARPLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            23861..>24460
                     /locus_tag="JN749_001956"
     CDS             23861..>24460
                     /locus_tag="JN749_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7368384.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5787 a         6159 c         6733 g         5781 t
ORIGIN      
        1 caagcaacaa tgaaaattgt ttatcgttat tatttccggg attaattacc aaaagcgaac
       61 aatggtttaa cctgctccat ttattcattt tatccaacca tgagcgaagc cgctcggcag
      121 gaatgttttg ccatgcgtta tttgcgcaaa caagaataaa aaggtaatta tggggatcaa
      181 tagaacactg caaatcacgg gccaaatagt atagaccttt ttcatgattt agcattgaaa
      241 ataattttat tttttccggc ccttgagaat catctaattg aaagattttc gccggatcgc
      301 tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg ggatgcaatt gtttgattcg
      361 ccagactgat agcatcttca tggcgatcga cgttaaacca ccagacgccg cctgctggca
      421 tatgtcgcag ctcatcccat aatgatgaga taccgataga gaatacaggg tccacaatgt
      481 ccctcatgtc accattttgt ctgattatca gtttactagc gaaagctaag aaataaacct
      541 accattgaaa ttaaaactta tcagatttag catgtgaatc aatttagaag gtcacaagac
      601 gtgtgatttt agtcttttcg tttttaactt tttctgttgc gtaacggaaa gtcaaaaagt
      661 gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa atgctcacgg ttagaattgt
      721 atcatgaatg gtacggttat ttcataggga tcaagcaaaa tgaataacaa tgaaccagat
      781 actctgcctg atcccgcgat aggctatatc ttccagaatg atattgtggc gttaaagcag
      841 gcattttcac tgcctgatat tgattatgcc gatatttccc aacgcgaaca gttggccgcg
      901 gcattaaaac gctggccgtt gctggcagag tttgcgcaac aaaagtaggg gattggtgaa
      961 tggccgtact gggattgcag ggggtgcggg gaggcgtggg gacaacaacc atcaccgccg
     1021 cattagcctg gtcattacaa atgttgggag aaaatgtcct agtggtcgat gcctgcccgg
     1081 acaacttgtt gcgcctgtca tttaatgttg attttaccca ccgtcagggc tgggccagag
     1141 cgatgctgga tggccaggac tggcgtgacg ctgggttgcg ctacacctcg cagctcgatt
     1201 tgctgccttt tggtcagtta tccattgaag aacaagaaaa tccacagcac tggcaaaccc
     1261 ggctgagcga tatttgctcc ggcttacagc aactaaaagc cagcgggcgt taccagtgga
     1321 ttttaatcga cttaccgcgt gatgcctcgc agataaccca ccagctgctg agtttgtgcg
     1381 agcactcgct ggcaatcgtc aatgtggatg ccaactgcca tatccgactg catcagcaag
     1441 cgctgccgga tggcgcacat attttgatta atgacttccg tattggcagt caggttcagg
     1501 acgatattta ccagctttgg ttgcaaagcc agcgccgatt actgccgatg ctcattcatc
     1561 gtgatgaagc gatggctgaa tgcctggcgg ctgaagagat actgacgctg gcgaactggt
     1621 gcctgttgaa ctactccggg ctgaaaacgc cagtcgggag tgcatcatga gtatcctgac
     1681 ccggtggttg cttatcccgc cggtcaacgc gcggcttatc gggcgttatc gcgattatcg
     1741 tcgtcacggt gcgtcggctt tcagcgcgac gctcggctgt ttctggatga tcctggcctg
     1801 gatttttatt ccgctggagc acccgcgctg gcaacgtatt cgcgcagaac ataaaaacct
     1861 gtatacgcca acgcctcgcg tccgcgtccg ctggacccgg tccgttatct cattcaaaca
     1921 tgctggttat tgatcggtgc atcgcgcaaa gaaacgccga aaccgcgcag cgggcatttt
     1981 caggtctgca aaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg
     2041 ttagccataa aacacagcat ctggatgaaa aaaaagagct cggtcatttg agtgccgggg
     2101 cgcggcggtt gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg
     2161 ttactcagcc gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggggtag
     2221 cgctgatcgt acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc
     2281 tgaccgtttc ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc
     2341 cggtcagcct ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc
     2401 tggtgctcgg ctacttccag gtagtatggc cgctgaatcg tcagccggtg ccattgccga
     2461 aggatatgtc gctgtggccg tcagtggata tctttgtccc gacttacaac gaagatctca
     2521 atgtggtgaa aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga
     2581 acatctggat cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg
     2641 gggtgaaata tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg
     2701 cgctgaaata tgccaaaggc gagttcgtgt cgattttcga ctgcgaccac gtaccaacgc
     2761 gatcgttcct gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc
     2821 agactccaca ccacttcttc tcgccggacc cgtttgaacg caacctggga cgttttcgta
     2881 aaacgccgaa cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg
     2941 acgccacttt cttctgcggt tcctgtgcgg tgattcgtcg taagccgctg gatgaaattg
     3001 gcggcattgc tgtcgaaacc gtgactgaag atgcgcatac ttctctgcgt ttgcaccgtc
     3061 gtggctatac ctccgtgtat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc
     3121 tgtcggcgca tatcggtcag cgtattcgct gggcgcgcgg gatggtacaa atcttccgtc
     3181 tcgataaccc gctcaccggt aaaggactga agtttgctca gcggctgtgc tacgtcaacg
     3241 ccatgttcca cttcttgtcg ggcattccac ggctgatctt cctgactgcg ccgctggcgt
     3301 tcctgctgct tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctattcgtgc
     3361 tgccgcatat gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact
     3421 ctttctggag tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg
     3481 tggcgctgat taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg
     3541 aagaagagta cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctactcaacc
     3601 tggtgggcgt tgcggtaggc atctggcgct acttctatgg cccgccaacc gagatgctca
     3661 ccgtggtcgt cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg
     3721 cggtatcggt agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg
     3781 cggcaattgc ccgcgaagat ggtcacctct tctcgtgtac cgttcaggat ttctccgacg
     3841 gtggtttggg gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc
     3901 tgttgcttaa acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg
     3961 gtaatgaagt tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc
     4021 agtgtacgtt tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata
     4081 agccgctgga aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg
     4141 cggagtttgc gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct
     4201 gggttgtatc gtttattccg cgccgcccgg agcggagcga aacggcacaa ccatcggatc
     4261 aggctttggc tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca
     4321 gtggctatgg ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa
     4381 ccactgatca atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg
     4441 caagtgatgt caggcgagca gggagctgat gcccccatcg tggcgcagaa cggtccttcg
     4501 cgtgatgtga agctgacctt tgcgcaaatc gcgccgccgc cgggcagcat ggtgctacgt
     4561 ggtattaacc cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag
     4621 gcgatgctca acctcgaata cacgccatcg ccatcgttac tgcctgtcca gtcgcagtta
     4681 aaggtttatc tcaatgatga gctgatgggc gtgctgccag tgaccaaaga acagttgggt
     4741 aaaaaaacgc tggcgcaaat gcccattaat ccactgttta ttaccgactt caaccgtgtg
     4801 cggctggagt ttgtcggcca ttatcaggac gtgtgcgaaa acccggccag caccacgctt
     4861 tggctggatg ttgggcggag cagtggactg gatctgacct atcagaccct gaatgtgaag
     4921 aatgacctgt cacacttccc ggtgccattc tttgacccgc gcgataaccg tactaacacc
     4981 ttgccgatgg tctttgcggg tgcgccggat gttgggctgc aacaagcatc tgccattgtc
     5041 gcctcgtggt ttggttcgcg ttctggctgg cgtgggcaga acttcccggt gctctataac
     5101 caattgccgg atcgcaatgc cattgtcttt gcaaccaacg acaaacggcc ggacttcctg
     5161 cgcgatcatc cggcggtaaa agccccggtg attgagatga ttaaccatcc gcagaatcct
     5221 tacgtcaaac tgctggtggt gtttggtcgt gacgacaaag acctgttgca ggcagcgaaa
     5281 ggtatcgctc agggtaacat tctgttccgt ggtgaaagcg tggtagtgaa tgaagtgaaa
     5341 ccgctgctac cgcgtaagcc gtacgatgcg ccgaactggg tacgtaccga tcgtccggtc
     5401 acatttggcg aactgaaaac ctatgaagaa cagttacaat ccagcggcct tgagccagca
     5461 gcgattaacg tttcgctaaa cctgccgccg gatctctacc tgatgcgcag taccggcatt
     5521 gatatggata ttaattaccg ctacaccatg ccgccggtga aagacagttc gcggatggat
     5581 atcagcctga ataaccagtt cctgcaatcc ttcaacctga gcagcaaaca ggaggcgaac
     5641 cgtctgctgc tgcgtattcc ggtattacaa ggtttgctgg atggcaaaac agatgtctct
     5701 attccggcgc tgaaactggg cgcgaccaac cagctgcgct tcgactttga gtatatgaac
     5761 ccgatgccag gcggttcggt agataactgt attaccttcc agccggtgca gaatcatgtg
     5821 gtgattggtg acgattccac catcgacttc tcgaagtatt accacttcat tccgatgccg
     5881 gatctacgcg cctttgctaa cgcgggcttc ccattcagcc ggatggcgga tctgtcgcaa
     5941 accatcaccg tgatgccgaa agcgcctaac gaagcacaga tggaaacgtt gctgaatact
     6001 gttggtttta tcggcgcaca gacgggcttc ccggcgatta atctgacggt gaccgatgat
     6061 ggcagcacca ttcagggcaa agatgccgac atcatgatca tcggtggtat cccggacaaa
     6121 ctgaaagacg ataagcagat cgacctattg gtgcaggcga ccgaaagctg ggtgaaaaca
     6181 ccgatgcgcc agaccccgtt ccccggcatt gtgccggacg agagcgatcg cgcggcagaa
     6241 acccagtcaa cgctgacctc ttccggtgcg atggcggcgg tgattggctt ccagtcgccg
     6301 tataacgacc agcgcagcgt gattgcgctg ttggcagata gcccacgcgg ttatgaaatg
     6361 cttaacgatg cggtgaacga tagcggcaaa cgcgccacca tgttcggttc ggtcgcggtg
     6421 atccgcgagt acggtatcaa cagcctacgt gttggcgacg tttattacgt aggtcatctg
     6481 ccgtggttcg agcgcttgtg gtatgcgctg gcaaaccatc cgattctgct ggcggtgctg
     6541 gcggctatca gtgtgatatt gctggcatgg gtactgtggc gtctgctgcg aattattagt
     6601 cgtcgtcgtc ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggaatc
     6661 gtgacgatgc tgttgttggc tgcctttagt gttcaggcag cctgtacctg gcctgcctgg
     6721 gagcagttta aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg
     6781 cgcaaaatca ccacctccga agggcaaagt tacggtatgt tctttgccct ggcggctaac
     6841 gaccgtgcag ctttcgataa tattctcgac tggacgcaga acaatctcgc tcagggttct
     6901 ttaaaagaac gtttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg
     6961 ctggacagca attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg
     7021 gggcgtttgt ggaaagagca gcgttatacc gacatcggca gcgcgttgct aaaacgtatc
     7081 gcgcgggagg aagtggtgac ggtgcctggg ctgggttcca tgttgttacc gggcaaagtg
     7141 ggttttgctg aggataacag ctggcgtttt aaccccagct acatgccgcc gacgctggcg
     7201 cagtatttca cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacattta
     7261 ttgctggaaa ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa
     7321 ggctggcagc taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac
     7381 atgtgggtag gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt
     7441 aaaccgatgg cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct
     7501 acggggaaag cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcctttttta
     7561 caaaaccgcg atgcgcaggc agttcagcgc cagcgcgtgg ccgataactt tcccggcagc
     7621 gatacctatt acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc
     7681 cgcttctcga caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac
     7741 taaacatatt cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa
     7801 ccatcagcag accctgcgga tggacagcgg caacactaat gccgtgcgcg ggctggcgaa
     7861 tatttaccgc cagcagtcgc cagaaaaagc tgaagcgttt atcgcctcgc tctctgccag
     7921 ccagcggcgc agcattgatg atatcgaacg cagcctgcaa aacgaccgtc tggcacagca
     7981 ggcagaggta ctggaaaacc agggcaaatg ggcgcaggcg gcagcacttc agcggcaacg
     8041 actggcgctg gaccccggca gcgtatggat tacttaccga ctttcgcagg atctctggca
     8101 ggccggacaa cgcagccagg ccgatacgtt aatgcgcaat ctggcgcagc agaagccgaa
     8161 caacccggag caggtttacg cttacgggct gtacctctca ggtcacaacc aggacagagc
     8221 ggcgctggcg catatcaaca gcctgccgcg cgcgcagtgg aacagcaata ttcaggagct
     8281 ggttaatcga ctgcaaagcg atcaggtgct ggaaaccgct aatcgcctgc gagaaagcgg
     8341 caaagaggct gaagcggaag cgatgctgcg tcagcaacca ccttccacgc gtattgacct
     8401 cacgctggct gactgggcgt aacaacgacg tgattacacc gccgcccgca ctgcatatca
     8461 gaatgtcctg acgcgggagc caactaacgc cgatgccatt cttggtctga cggaagtgga
     8521 tattgctgcc ggtgacaaag cggcggcacg tagccagctg gcgaaactgc ctgccaccga
     8581 taacacctcg ctgaacactc agcggcgcgt ggcgctggca caggcgcagc ttggagatac
     8641 cgcagcggcg cagcagacgt ttaataagtt gatcccgcag gcaaaatctc agccaccgtc
     8701 gatggaaagc gcgatggtgc tgcgcgacgg tgcgaagttt gaagcgcagg cgggcgatcc
     8761 aaagcaggcg ctggaaacct acaaagacgc catggtcgca tccggtgtga ctacgacgcg
     8821 tccgcaggat aacgacacct ttacccgact gacccgtaac gacgagaaag atgactggct
     8881 gaaacgcggc gtgcgcagcg atgcggcgga cctctatcgc cagcaggatc ttaacgtcac
     8941 ccttgagcac gattactggg gttcgagcgg caccggtggt tactccgatc tgaaagcgca
     9001 cactaccatg ttgcaggtgg atgcgccgta ttctgacggg cggatgttct ttcgcagtga
     9061 tttcgtcaat atgaacgtcg gcagtttctc cactaatgcc gatggcaaat gggatgacaa
     9121 ctggggcacc tgtacattac aggactgtag cggcaaccgc agccagtcgg attccggtgc
     9181 cagcgtggcg gtcggctggc gaaatgacgt ttggagctgg gatatcggta ccacgccgat
     9241 gggcttcaac gtggtggatg tggtcggcgg catcagttac agcgatgata tcgggccgct
     9301 gggttacacc gttaacgccc accgtcggcc catctccagt tctttgctgg cctttggtgg
     9361 gcaaaaagac tccccgagca ataccgggaa aaaatggggt ggcgtacgtg ccgacggtgt
     9421 ggggctaagt ctgagctacg ataaaggtga agtaaacggc gtctgggcat cgcttagtgg
     9481 cgaccagtta accggtaaaa atgtcgaaga taactggcgc gtgcgctgga tgacgggcta
     9541 ttactataag gtcattaacc agaacaatcg ccgcgtcaca atcggcctga acaacatgat
     9601 ctggcattac gacaaagatc tgagtggcta ctcactcggt cagggcggtt actacagtcc
     9661 gcaggaatac ctgtcgtttg ccataccggt gatgtggcgg gagcgcacgg aaaactggtc
     9721 gtgggagctg ggggcgtctg gctcgtggtc gcattcacgc accaaaacca tgccgcgtta
     9781 tccgctgatg aatctgatcc cgaccgactg gcaggaagaa gctgcgcggc aatccaacga
     9841 tggcggcagc agtcagggct tcggctacac ggcgcgggca ttacttgaac gacgtgttac
     9901 ttccaactgg tttgttggca cggcaattga tatccagcag gcgaaagatt acgcacccag
     9961 ccatttcctg ctctacgtac gttattccgc cgccggatgg cagggtgaca tggatttacc
    10021 gccgcagccg ctgatacctt acgccgactg gtaagttttc agatagcgcc tctcttaatg
    10081 ccgctgcgat cgggtatact cgggcggcaa tctgggattt ccggggggag acaatttgcg
    10141 cgtaagtcgc tcgttaacaa tcaagcagat ggcaatggtg gcagccgttg tcctggtgtt
    10201 cgtttttatt ttttgcaccg ttttgctgtt ccatctggtc cagcagaatc gctataacac
    10261 ggctacgcaa ctggaaagca ttgctcgctc tgtccgcgaa cccttatctt cagctatttt
    10321 gaaaggcgat attcccgaag cggaagctat tcttgccagc attaaaccgg caggcgtggt
    10381 cagccgtgcc gatgtagtgc tgcctaacca gttccaggcg ctgcgtaaaa gttttattcc
    10441 agagcgtccg gtgccggtaa tggttactcg cctgtttgag ctaccggttc aaatctcgct
    10501 gggcgtctac tcgctcgaac gtccggcaaa tccgcagcca atagcctatc tggtgctaca
    10561 ggcggattcc ttccgtatgt ataagttcgt gatgagcact ctctcaacgt tagtgaccat
    10621 ttacttactt ttgtcgctta tcctgaccgt cgccatcagc tggtgcatta accgcctgat
    10681 tttgcatccg ttacgcaata ttgctcgcga acttaacgcc atcccagccc aggagcttgt
    10741 tggtcaccaa ctggcattac cgcgtctgca tcaggacgat gaaatcggta tgttggtgcg
    10801 cagttacaac ctcaaccagc aattgctgca gcgccattat gaagaacaga acgaaaatgc
    10861 gatgcgcttc ccggtgtcgg atttgccgaa caaagccttg ctgatggaga tgctggagca
    10921 ggttgtcgcg cgtaaacaaa ccaccgcgct gatgatcatc acctgtgaaa ccctgcgtga
    10981 tactgcgggc gtgctgaaag aggcgcaacg agaaattctg ctgctgacgc tggtggaaaa
    11041 actcaaatcg gtactgtcgc cacgtatgat cctcgcgcag attagcggtt atgactttgc
    11101 tgtcattgcc aacggtgtac aggaaccgtg gcacgcaatc actttgggtc agcaagtact
    11161 cactatcatg agcgagcgcc tgccgattga acgtattcaa ctccgtccgc actgtagcat
    11221 tggcgtggcg atgttctacg gcgatctcac cgccgaacag ctttacagtc gcgctatttc
    11281 tgcggcattt accgctcgcc ataaaggcaa gaatcagatt cagttctttg atccgcagca
    11341 gatggaagca gcccagaagc ggttgacgga agagagcgat atccttaatg cactggaaaa
    11401 tcatcagttt gcaatttggt tacagccaca ggtcgagatg accagcggta aactggtcag
    11461 tgcggaagtg ttactgcgta tccagcaacc ggatggcagt tgggacctgc cggatggctt
    11521 aatcgatcgc attgagtgct gtgggctgat ggttaccgtc ggtcactggg tgctggaaga
    11581 gtcctgtcga ttgcttgcag cctggcaaga gcgcggcatt atgctgccct tgtcggtaaa
    11641 cctctctgcg ctgcaactga tgcacccgaa tatggtggcg gatatgctgg aactgttaac
    11701 ccgctatcgc attcagccgg gaacactgat tctggaagtg acagaaagcc gacgtattga
    11761 cgaccctcat gctgcggtgg caatcctccg tccgctgcgc aatgccggag ttcgggtggc
    11821 gctggatgat ttcggcatgg gctacgcagg gctgcgtcag ctgcagcata tgaaatcgtt
    11881 gccaatcgac gtactgaaaa tcgacaaaat gtttgttgaa ggcttgccgg aagatagcag
    11941 catgattgct gcaattatca tgctggcgca gagcctgaac ttacaaatga ttgccgaagg
    12001 cgtggagact gaagcacaac gagactggct ggcaaaagcg ggcgttggta ttgcccaggg
    12061 cttccttttt gctcgcccac tccctattga aatcttcgaa gagagttacc tggaagaaaa
    12121 gtagctaccc caaactgatt acaaaacttt aaaaagtgct ggtttgtgcg agccagctca
    12181 aactttttaa cctttttgtt tcaattatga tccaggtaca tttctgtgat gttgtctggg
    12241 tgttatttta aggccgcagg tacccccata accttacaag acctgtggtt ttactaaagg
    12301 acaccctatg aaaacctctc tgtttaaaag cctttacttt caggtcctga cagcgatagc
    12361 cattggtatt ctccttggcc atttctatcc tgaaataggc gagcaaatga aaccgcttgg
    12421 cgacggcttc gttaagctca ttaagatgat catcgctcct gtcatctttt gtaccgtcgt
    12481 aacgggcatt gcgggcatgg aaagcatgaa ggcggtcggt cgtaccggcg cagtcgcact
    12541 gctttacttt gaaattgtca gtaccatcgc gctgattatt ggtcttatca tcgttaacgt
    12601 cgtgcagcct ggtgccggaa tgaacgtcga tccggcaacg cttgatgcga aagcggtagc
    12661 ggtttacgcc gatcaggcga aagaccaggg cattgtcgcc ttcattatgg atgtcatccc
    12721 ggcgagcgtc attggcgcat tttccagcgg taacattctg caggtgctgc tgtttgccgt
    12781 actgtttggt tttgcgctcc accgtctggg cagcaaaggc caactgattt ttaacgtgat
    12841 cgaaagtttc tcgcaggtca tcttcggcat catcaatatg atcatgcgtc tggcacctat
    12901 tggtgcgttc ggggcaatgg cgtttaccat cggtaaatac ggcgtcggca cactggtgca
    12961 actggggcag ctgattatct gtttctacat tacctgtatc ctgtttgtgg tgctggtatt
    13021 gggttcaatc gctaaagcga ctggtttcag tatcttcaaa tttatccgct acatccgtga
    13081 agaactgctg attgtactgg ggacttcatc ttccgagtcg gcgctgccgc gtatgctcga
    13141 caagatggag aaactcggct gccgtaaatc ggtggtgggg ctggtcatcc cgacaggcta
    13201 ctcgtttaac cttgatggca catcgatata cctgacaatg gcggcggtgt ttatcgccca
    13261 ggccactaac agtcagatgg atatcgtcca ccaaatcacg ctgttaatcg tgttgctgct
    13321 ttcttctaaa ggggcggcag gggtaacggg tagtggcttt atcgtgctgg cggcgacgct
    13381 ttctgcggtg ggccatttgc cggtagcggg tctggcgctg atcctcggta tcgaccgctt
    13441 tatgtcagaa gctcgtgcgc taactaacct ggtcggtaac ggcgtagcga ccattgtcgt
    13501 tgctaagtgg gtgaaagaac tggaccacaa aaaactggac gatgtgctga ataatcgtgc
    13561 gccggatggc aaaacgcacg aattatcctc ttaatctcac cacttatgcc cgcactccct
    13621 tccccgagtg cgggtgtttg cgcataattg acccctgtgc ctgaacttac cacctgggtg
    13681 agttcaggtt tatttcactt cttccgtgac attttcatgt tcttgcggtc taacacgaag
    13741 tgtttttaac gtcatttaga ctgaccgaca acgggcagtt tttttattac caggatagtt
    13801 gatcaggggt tcacatgcag ggcataaaaa ttcgactttt agcgggcggt ttgctgatga
    13861 tggccactgc tggctatgtg caggcagatg cgctccagcc tgatccagca tggcaacagg
    13921 ggacgctttc caacggttta cagtggcaag tgctgaccac cccccagcgt cccagcgatc
    13981 gtgttgaaat tcgcctgctg gttaataccg gttcgctcgc cgaaagtaca caacagagcg
    14041 gttacagtca cgccatccct cgtattgcgc taacgcaaag cggtggcctt gacgcagcac
    14101 aggcgcgttc attgtggcag caggggatcg accctaaacg cccgatgccg ccggtaattg
    14161 tctcttatga caccacgctg tttaatctga gtttgcccaa taaccgtaac gatttgctga
    14221 aagaagcgct ctcttatctg gcaaatgcca ctggcaaatt gaccatcaca ccagaaacca
    14281 tcaaccacgc gctgcaaagt caggacatgg tggcaacctg gcctgccgat actaaagagg
    14341 gctggtggcg ctatcgtctg aaagggtcaa ccttgttagg tcacgatcct gccgatccgc
    14401 tgaaacaacc cgttgaagcg gaaaaaatta aagatttcta tcagaaatgg tacaccccgg
    14461 atgcaatgac gctactggtg gtgggaaacg tggatgcgcg ctcggttgtc gaccaaatca
    14521 acaaaacgtt tggcgaactg aaaggcaaac gtgaaacgcc agctccggtg ccgacgcttt
    14581 ctccgctgcg tgcggaagcg gtgagtatta tgactgacgc ggtgcgtcag gaccggttat
    14641 ctatcatgtg ggatacgccg tggcagccga ttcgtgaatc agccgcactg ctgcgctact
    14701 ggcgtgcgga cctggcccgc gaggcgctgt tctggcatgt tcagcaagcg ttaagcgcca
    14761 gtgacagcaa agacatcggt cttggatttg actgccgtgt gctgtatctg cgtgcgcagt
    14821 gtgccatcaa catcgaatca ccaaacgaca agctgaacag caaccttaat ctggtggcgc
    14881 gtgaactggc gaaggttcgc gataaaggtc tgccggaaga agagttcaat gcgttagtgg
    14941 cgcaaaagaa actggagctg cagaaactgt ttgccgccta tgcacgggct gataccgata
    15001 ttctgatggg ccagcggatg cgttcgttgc aaaatcaggt cgtcgatatc gcgccggagc
    15061 agtatcagaa actgcggcag gatttcctta acagcctgac tgtggagatg ttaaatcagg
    15121 atctgcgtca gcagttgtcg aatgatatgg cgttaatact gctgcagccg aaaggcgagc
    15181 cggaatttaa catgaaagcg ttgcaggcgg cctgggatca aatcatggcc ccatctaccg
    15241 ccgctgcgac cacctctgtc gccacggatg acgtacatcc tgaagtgacg gatattccac
    15301 ctgcacagta atgcctgatg gtgatgagtt agcacaacca tcaggtattt atggatgagc
    15361 tggatagagg ggtaacactt tatccagcct tttgcatatg ctgcgtttac gctggcatcg
    15421 cctcacgcgg gataatcgcg ccgcgatact gaataacggt acttgcggtc aggtgcccgc
    15481 gtttcgccgc gttttccgca ctgccgcccg tcaaacgtac cgccagataa ccggcgctga
    15541 aagagtcgcc agctgcggtg gtatcgatca ctttttcttt cggcagtttc accgccggaa
    15601 catcaactaa cgcttcacca gcaatggaca ccaggcaaga atccgccccg cgtttcacca
    15661 ccacttcttt cacgcccgcg ttatgggtgc gcgcaatgac gtcttccacc ggctgttgac
    15721 cccacagcgc gtcttcgtcg tccagcgtca ggaaggcgat atccgtgcat tccagcattt
    15781 gctggtacac ctgctgtgtc tcttctttgc tggcccacag gcgcggacga tagttattgt
    15841 cgaaaatcac ttttccgcct ttggcgcggc attcgcgcag cagggaaagc agcttttcgc
    15901 ggctggtcgg gcttaagatc gccaggctaa tcccgctcag gtagagataa tcgaaattcg
    15961 ccagctcttc gcaaatcgcc gcagactgct cgctcgccag ccagaatttg gcggcggctt
    16021 cgttacgcca gtagtagaac gtacgttcgc cggtgctgtc ggtttcaatg tagtaaaggc
    16081 ccggaagacg gttttccatc cgttgggtca gggaagtatc aacgttctcg ccgtgccagg
    16141 cgtccagcat ctgctggcta aaactgtccg ttcccagcgc cgttacgtaa tgaacggtta
    16201 atgccgcagg atcgacctga cgggcgatat agacggaagt gttcagggta tcgccgccga
    16261 aaccgcgctt aacgtccgcg tctttctcgg aaagctcaat catgcattcg ccaatcacgg
    16321 caatcttttt ggacatagtc gtgaacctga tctgtaaaaa attagcgtta gtttgcgctg
    16381 tggtggtttg ctggtcaacc atattaaaac agtgttccat tatttttttg agccagaacg
    16441 tattcctgaa agattccgtt gtggagtgag gaaatttacc ccggccaggc ggtaaagttc
    16501 tgcccttacg cgccgataat ctttgtcgag tccgggcagc atcactttta aacacaggac
    16561 atctttgatg ataaggcagg ttatccagcg aataagcaac cctgaagcaa gcatcgagag
    16621 cttgcaggaa cggcgttttt ggttgcagtg tgagcgtgct tacacctggc agccgatcta
    16681 tcaaacatgc gggcggttaa tggccgtgga gctattaacg gtggtcacgc atcccttgaa
    16741 cccttcgcaa cgcctgccgc cggatcgcta ttttactgaa atcaccgtca gccatcggat
    16801 ggaggttgtg aaagagcaga ttgatttgct ggcgcaaaaa gccgacttct ttgtagagca
    16861 cggcctgctg gcatcggtca atattgatgg ccctacgctc atcgccctgc gtcagcaacc
    16921 aaaaatcctg cgccagattg agcgtcttcc ctggctgcgt ttcgaactgg tggagcatat
    16981 ccgtctgccg aaagattcaa cctttgcctc gatgtgtgaa tttggcccgc tgtggctgga
    17041 tgattttggt accgggatgg caaatttctc tgcactaagt gaagtgcgtt atgactacat
    17101 caaaatcgcg cgagaactgt ttgtgatgct gcgtcagtcg ccggaaggcc gcacactctt
    17161 ttctcagctt ttacatctaa tgaatcgcta ttgtcgcggg gtgattgtcg agggcgtaga
    17221 aacaccggaa gagtggcgtg atgttcagaa ctcgcccgca ttcgccgcac aaggctggtt
    17281 tctttcacgc ccggcaccga tggaaacgct gaatacggcg gttctggcgc tataagctgc
    17341 ctcattttcc gccgggctgg actatcttta ggactggcac aggaagaata tgaggaagcg
    17401 aacgatgagc aaggcaggca aaataaccgc tgcgatttca ggggttttct tgttgttgat
    17461 tgtcgtggcg atcattttga ttgcaacttt tgactggaat cgactcaaac cgaccatcaa
    17521 ccagaaagtc tctgcggagt tgaatcgtcc gttcgctatc cgtggcgatc tgggcgtggt
    17581 gtgggagcgg caaaaacaag aaactggctg gcgcagctgg gtgccgtggc ctcatgtaca
    17641 tgcagaagac atcattcttg gtaatccacc ggatattccc gaagtcacga tggtgcattt
    17701 gccgcgagta gaggcaacgc tggcaccgct ggcgctgctg accaaaacgg tctggctgcc
    17761 gtggatcaag ctcgaaaagc ccgacgcgcg cctgattcgc ctctctgaaa agaacaataa
    17821 ctggacgttt aatcttgcca acgatgacaa caaagacgcg aatgcaaagc cgtcggcatg
    17881 gtcgtttcgg ctggataata ttcttttcga tcaagggcgg atcgccattg atgacaaagt
    17941 aagcaaagcg gatctggaaa tttttgttga tccgttaggc aagccgctgc cattcagcga
    18001 agttactgga tcgaaaggta aagcggataa agaaaaggtg ggcgattaca tttttggcct
    18061 gaaggcgcag ggacgttata acggtgaacc gctcacgggt acgggaaaaa taggcggtat
    18121 gctggcgctg cgtggcgaag ggacgccgtt tccggtacag gctgatttcc gttcaggtaa
    18181 tacccgtgtt gcttttgatg gcgtcgtgaa tgacccaatg aagatgggcg gtgtcgattt
    18241 acggcttaaa ttttctggcg attcactggg tgatctctat gaactgacgg gcgttctgct
    18301 gcccgatacc ccgccgtttg aaacggatgg tcggctggta gcgaaaatcg acactgaaaa
    18361 atcgtcggtc tttgattatc gcggttttaa tgggcgaatt ggcgatagcg atatccacgg
    18421 ttctctggtc tacaccaccg gaaagccacg accaaaactg gaaggtgatg tcgagtcgcg
    18481 gcaattgcgg ctggcggacc tgggaccgtt gattggcgtt gattccggga aaggggcaga
    18541 aaagtcgaaa cggtctgaac agaagaaggg cgaaaaaagc gttcagcctg cgggcaaagt
    18601 gctgccttat gaccgcttcg aaaccgataa atgggacgtt atggatgccg atgttcgctt
    18661 caaagggcgg cgcattgagc atggcagtag cctgccgatt agcgatcttt ctactcatat
    18721 catcctcaaa aatgctgacc tgcgcctgca accgctgaaa tttggcatgg cgggcggcag
    18781 cattgcggcg aatattcatc tggaaggcga taaaaagccg atgcaggggc gggcagatat
    18841 tcaggctcgt cgactgaaac tgaaagaact gatgcccgat gtggaactga tgcagaagac
    18901 gctgggggaa atgaacggtg acgcggaact acgcggtagc ggtaactcgg tggcggcgct
    18961 tttaggcaac agtaacggca acctgaaact gttgatgaat gacgggctgg tgagccgcaa
    19021 cctgatggag attgttgggc tgaatgtcgg caactacatt gtcggtgcga tatttggtga
    19081 cgatgaggtg cgggtgaact gcgcggcggc gaatctgcat attgccaacg gcgtggcgcg
    19141 cccgcagatt tttgctttcg atactgagaa cgcgttgatt aatgttaccg gcacggcaag
    19201 ttttgcttcg gaacagctgg atttgactat tgatccggag agtaaaggga ttcggattat
    19261 cacactgcgt tcgccgctgt atgtgcgtgg gacgtttaaa aatccgcagg ctggggtgaa
    19321 agccggaccg ctgattgccc gtggtgctgt tgctgcggca ctggcaacgc tggtaacacc
    19381 ggcggcggcg ttactggcac tgatctcacc ttccgaaggg gaggctaatc agtgtcggac
    19441 gatattgtcg cagatgaaga agtgatgata attgccggat gtctgacatc cggcaattaa
    19501 acagatttag atttttggca aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt
    19561 atccggccta caaatccttg tgaattcaat atattgtatg gaaattcagt cctgataagc
    19621 gtagcgcatc aggcttttca cacttattac aacgactgat gtcgcgtctc atgggtcagc
    19681 agcagggcga ttaacgtcaa gccagccatc gccgccagat ataaccccac cgcacctaac
    19741 ccgtagttag cctgcaacca agctgcgata tacggcgcaa cggaagcccc gagaatcgac
    19801 gatacgttgt aagagaacga tgctccggtg taacgcactt ctgtcggaaa cagctctggt
    19861 aacagcgcac ccatcggccc gaaggtcagc cccatcagac ttaaccccag cagcaggaag
    19921 gcaaaaacca gaatcgggtt gccagaaccg agcagcgggt taaaggcgaa cagcgcgaac
    19981 aggatgatca gcgtggtgat gatcaccatg cttttacggc gaccaaaggc atcagccagt
    20041 aatccggcga ctggcaccat cacgccaaaa ccaataactg ccatcatcag catccacaac
    20101 acttcgttac gcggcaggcc aagcccaact ggcgcggcgg cggtactgaa ggtcatagag
    20161 tagaccgtca tgatgtaaaa cagcgtatag gttgccagca taatgaacgt acccagtacg
    20221 gttacgcgaa catgtttggt cagcagcgta cccagcggga tcttcacctg ttttttcgct
    20281 ttagcgactt tctcaaacac cggcgactca tgcagcgaca cgcgaacata caggccgata
    20341 atgaccagca ccgccgagaa gataaatggc acacgccagc cccagctcat aaactgctca
    20401 tcggtcagta gccaggaaag cagcaagaaa gtgccgttgg caaagaagaa gccaatcggt
    20461 gcgcctagct gtgggaagga gccatacagt gcacgtttgc gcggtggggc gttttcagtc
    20521 gccagcagcg ccgcgccgcc ccattcaccg cctaagccca gaccctgacc aaatcgagcc
    20581 agcgccagca gcagtggggc gaaaatacca atcgtggcat agcccggcag cagaccaatc
    20641 accacggtcg aaatccccat cgttagcaac gaggcgacca gcgtcgcttt acgcccaacg
    20701 cgatcgccaa aatgaccaaa aacggcagag ccaatggggc gcgcgacgaa ggcgatggcg
    20761 aaggtggcga gcgactgtag cgttgctgcc gttggatcgc cctgcgggaa gaagatatgc
    20821 ggaaacacaa taacggccgc agtggcgtaa atgtaaaagt cgaagaactc aatggccgtg
    20881 ccaatgagag aggcgacaag gactttatta cgcgagttga tcggcgtgta ttcttgttcg
    20941 tggtcgagtg ttgtggctgt tgcttgcata atcttttctt attttttggc taacgaatag
    21001 ccatattacg cacagcaaaa gtgatatttc aatgtgtaac aaatcgcgga aatggtcaaa
    21061 aaccaggcaa aaaggggata atgtttaggc cagtgatttt tcttctatga aatgcttcac
    21121 ataattttat aaatagtgga taaaactggt ttcaggttaa ataaaagtta ccaactggat
    21181 ttatatgctg gggttatgag ttggagtgaa attgtttgct cccaaataga tggctgaaga
    21241 ggctctgggg aagagtttca agaggttatg tgcgcattat agccggatgc gatgctggcg
    21301 catcttatcc ggcctacggg gagccaggcc gaataaaatc gtatgccgga taaggcgttt
    21361 acgccgcatc cggcctattt taaggctgcg ttttccctgg cattcgcggg tcgtctttat
    21421 attcggcggt ggcaatccac gccgcgcaaa acagcgtcaa acgggcgaag aagtagaaaa
    21481 atgccatcag ccccagcacg gaaccaaatg ctgcgccaga gggggatttc atcagcgatg
    21541 gcagggtgta ggtcatcacg attttaatca cttcaaaacc aatagcagcc agaaatgttc
    21601 cgcgaatcag ggcttttttg cgcggacggt ggcgcggcaa ccgccagaag atccagaaga
    21661 aaagcagata gttggcgaag atggaaatcg ccaggccaat caatcgccac gtcggtttca
    21721 gccactcaat gctgttcagg tgcagggcgc taataatcat ttgctgcgcc gaaccggcaa
    21781 ccgaggtgat cgaaagcgtc acaatcagcg caatcaacaa accaatcagt gaaataaaat
    21841 cacgcagata ttttacccag aacttctcct gatcttgcgg cgagcgttcc cagacatcgc
    21901 gcgactgggc acgaatcgct tcacgcaggt tacccatcca gttgatgccg gaataaagtg
    21961 ccaccgccag gccgacaagc cctacagtcg tacgctgctg aacggcggtg ttgatggtgt
    22021 ttttcaacgt ggcggctagt gtcggatcgc tgatgttttg cagaattttg tcgaagatat
    22081 cctgtagcaa catcggatgg gaggccagca caaagccccc tgcggcaaac gacaccatca
    22141 aaatcgggat catcgacaaa aatgagaaat aggtgatagc cgcaccaaac tggttaccca
    22201 gccgatcgtt aaagcgttct gtcgcgcgaa taaggtgcgc aataaccggt tggcgttgga
    22261 ctttttcggc agtggtggtg acggtttcca gcgcctggct aactttgccg cctttctcgc
    22321 tattatccaa ctgcttaatc ggctcgtgct ccagatcctg ggtgggacgt ttgatctcgt
    22381 tttcctgcgt catccgctct tttgtccttc tgtcgtggtc attcttaaaa agtatagtcg
    22441 gtcagtccag gtgctctttc attacgccag ccagccactg cataaacagg tttacacgcc
    22501 gggaaagctc ccgacgctgc gggtaaacca gcgaaaggga gagcggctcg gcacggtagc
    22561 ctggcaacac ttcaataagc cgcccggcac gcagggcttc gcgcacggca atgcgcggga
    22621 tctgaataat ccccagaccg gcaagaccgg cggcgagata gttttcgcta ctgtttaccg
    22681 tcaacatgcc gccagactta aaccactgga cgccattaac gctggcaacc tcaaaaccta
    22741 acggatgtac acccaggtgc ggtgtataac gcactattgc gtgtgaagtc agatcatcgg
    22801 ggctttgcgg ataaccaaag cgcgtcagat agtgcggact ggcacagttg accatggtta
    22861 gtttgccgag gggacgggcg ataacgccat cttccggtaa cgcgccagtg cgtatcacgc
    22921 aatcaaaacc atcatgaaga atatctaccg gacggttatg gctactcagt tccagctcaa
    22981 ttcccggatg ctgatagaga aattccgaca gacgcggcag taacaggttt ttcgcgattc
    23041 ctgacgggat gtcgatgcgt aatttaccgc tgatactggt ggcatcctgt tgaaacagac
    23101 cgtccagttc gctgagatta ctcaacacat cttttgcccg ttgataatag gtcatgcctt
    23161 ctggcgtgag tttgacccgt cgcgtagtgc gctggagaag ctgggtgcca agttgatgtt
    23221 ccagtgcctg tatctggcgc gaaacacttc cctttggcaa agcaaagaaa tccgccgcgc
    23281 gggaaaaact ttccagctcc gcgactttga tgaacaactg cattgcgtga attttatcca
    23341 tttctttgac cgattgttgt tcacagcgaa acagtaacgc gcatatcgca ttgtttattg
    23401 ttttttctaa catctaatgc cgggtttcac aatgttgtca tgacgaatgt ggcgatttct
    23461 cagcaggaaa agggcggaat agctttatga gaaaggtgaa tccccttaaa cagagcgtat
    23521 ttttcagtga ttccctttaa agtgaataga ggacgtattg gctatttgtg cttatttttg
    23581 tctcattgcc atttgttatt ttggcttgta gtcgcggaat ttcattttta attgcatgcg
    23641 tactaaccac caatataagg aaatcctcaa aattgtttta aatagtgccc tcgacatgta
    23701 atttcaggcg tttagctgta actatttata tcggtaacta ttttctacgc gtcatctctg
    23761 tagaatggcc accgccaaat ggcgaattct tattttttct cgcgggtaat acccttccac
    23821 tacttaagtc tttttaacgt attcaggtgg agattcagtt atgcaaatag tcatgtttga
    23881 caggcagtca atatttattc atggaatgaa aatcagttta cagcagcgta ttccaggagt
    23941 gagtattcag ggggtcagtc aggcagacga gttatggcaa aagctggaaa gttaccctga
    24001 agccttagtt atgctcgatg gcgatcagga tggtgagttt tgctactggt tgctgcaaaa
    24061 aaccgtggtg cagtttcctg aggttaaggt gttaatcacg gcgacggatt gcaataaacg
    24121 gtggttacag gaagttatcc attttaatgt gctggccatt gtgcctcgcg attcaaccgt
    24181 cgagacattt gcgttggcgg taaatagtgc ggcgatgggg atgatgtttc tgcctggaga
    24241 ctggcggacg acgccggaaa aggacattaa agatctcaaa tcgttaagcg cccgccagcg
    24301 ggagatttta accatgttag cagcgggcga atcaaataag gagatcggca gagcgctgaa
    24361 tatcagtacc ggaacggtaa aagcgcatct ggaatccctg tatcgccgtc tggaagtgaa
    24421 aaatcgcacc caggcggcaa tgatgttaaa tatctcctcc
//
LOCUS       DADLTF010000052        24188 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.052, whole genome shotgun sequence.
ACCESSION   DADLTF010000052 DADLTF010000000
VERSION     DADLTF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.052"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(22..594)
                     /locus_tag="JN749_001957"
     CDS             complement(22..594)
                     /locus_tag="JN749_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368385.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="JN749_001958"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="JN749_001958"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HAY7368386.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JN749_001959"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="JN749_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HAY7368387.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JN749_001960"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="JN749_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAY7368388.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="JN749_001961"
     CDS             complement(3556..5337)
                     /locus_tag="JN749_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7368389.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="JN749_001962"
     CDS             complement(5330..7102)
                     /locus_tag="JN749_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HAY7368390.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /locus_tag="JN749_001963"
                     /pseudo
     CDS             complement(7132..7590)
                     /locus_tag="JN749_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JN749_001964"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="JN749_001964"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HAY7368391.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="JN749_001965"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="JN749_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="JN749_001966"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="JN749_001966"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HAY7368392.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /locus_tag="JN749_001967"
     CDS             complement(11173..11571)
                     /locus_tag="JN749_001967"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HAY7368393.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="JN749_001968"
     CDS             complement(11665..12036)
                     /locus_tag="JN749_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368394.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNLLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JN749_001969"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="JN749_001969"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7368395.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JN749_001970"
     CDS             complement(14250..14522)
                     /gene="hupB"
                     /locus_tag="JN749_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HAY7368396.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JN749_001971"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="JN749_001971"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HAY7368397.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JN749_001972"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="JN749_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HAY7368398.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JN749_001973"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="JN749_001973"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAY7368399.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JN749_001974"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="JN749_001974"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAY7368400.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JN749_001975"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="JN749_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HAY7368401.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="JN749_001976"
     CDS             21807..22385
                     /locus_tag="JN749_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7368402.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="JN749_001977"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="JN749_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HAY7368403.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6661 c         5837 g         6023 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataattgtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gtgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctgatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 caaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcgcgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcagtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgacggtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DADLTF010000053        24112 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.053, whole genome shotgun sequence.
ACCESSION   DADLTF010000053 DADLTF010000000
VERSION     DADLTF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.053"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1270
                     /gene="glpK"
                     /locus_tag="JN749_001978"
     CDS             <1..1270
                     /gene="glpK"
                     /locus_tag="JN749_001978"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAY7368404.1"
                     /translation="TNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDY
                     ISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVT
                     DYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPI
                     SGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGE
                     VNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLSAPY
                     WDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAV
                     ANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFR
                     PGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            1500..2510
                     /gene="glpX"
                     /locus_tag="JN749_001979"
     CDS             1500..2510
                     /gene="glpX"
                     /locus_tag="JN749_001979"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7368405.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLNRKDPEMQVH
                     IL"
     gene            2607..3353
                     /gene="fpr"
                     /locus_tag="JN749_001980"
     CDS             2607..3353
                     /gene="fpr"
                     /locus_tag="JN749_001980"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HAY7368406.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="JN749_001981"
     CDS             complement(3358..3786)
                     /gene="uspD"
                     /locus_tag="JN749_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HAY7368407.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLENKSDNKLYKLTKNIQCPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(3813..4112)
                     /locus_tag="JN749_001982"
     CDS             complement(3813..4112)
                     /locus_tag="JN749_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HAY7368408.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(4324..4764)
                     /locus_tag="JN749_001983"
                     /pseudo
     CDS             complement(4324..4764)
                     /locus_tag="JN749_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            4865..5464
                     /locus_tag="JN749_001984"
     CDS             4865..5464
                     /locus_tag="JN749_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HAY7368409.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            5572..6339
                     /gene="tpiA"
                     /locus_tag="JN749_001985"
     CDS             5572..6339
                     /gene="tpiA"
                     /locus_tag="JN749_001985"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAY7368410.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="JN749_001986"
     CDS             complement(6394..7149)
                     /gene="cdh"
                     /locus_tag="JN749_001986"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HAY7368411.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKSVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(7256..8245)
                     /locus_tag="JN749_001987"
     CDS             complement(7256..8245)
                     /locus_tag="JN749_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7368412.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="JN749_001988"
     CDS             complement(8565..9527)
                     /gene="pfkA"
                     /locus_tag="JN749_001988"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAY7368413.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="JN749_001989"
     CDS             complement(9708..10610)
                     /gene="fieF"
                     /locus_tag="JN749_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HAY7368414.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="JN749_001990"
     CDS             complement(10758..11258)
                     /gene="cpxP"
                     /locus_tag="JN749_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HAY7368415.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            11408..12106
                     /gene="cpxR"
                     /locus_tag="JN749_001991"
     CDS             11408..12106
                     /gene="cpxR"
                     /locus_tag="JN749_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HAY7368416.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            12103..13476
                     /gene="cpxA"
                     /locus_tag="JN749_001992"
     CDS             12103..13476
                     /gene="cpxA"
                     /locus_tag="JN749_001992"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HAY7368417.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="JN749_001993"
     CDS             complement(13582..14256)
                     /gene="yiiM"
                     /locus_tag="JN749_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HAY7368418.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="JN749_001994"
     CDS             complement(14405..15388)
                     /gene="kdgT"
                     /locus_tag="JN749_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HAY7368419.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="JN749_001995"
     CDS             complement(15648..16268)
                     /gene="sodA"
                     /locus_tag="JN749_001995"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HAY7368420.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            16553..17587
                     /gene="rhaT"
                     /locus_tag="JN749_001996"
                     /pseudo
     CDS             16553..17587
                     /gene="rhaT"
                     /locus_tag="JN749_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(17584..18522)
                     /gene="rhaR"
                     /locus_tag="JN749_001997"
     CDS             complement(17584..18522)
                     /gene="rhaR"
                     /locus_tag="JN749_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HAY7368421.1"
                     /translation="MAFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVPFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="JN749_001998"
     CDS             complement(18506..19342)
                     /gene="rhaS"
                     /locus_tag="JN749_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HAY7368422.1"
                     /translation="MTVLRSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            19630..21099
                     /gene="rhaB"
                     /locus_tag="JN749_001999"
     CDS             19630..21099
                     /gene="rhaB"
                     /locus_tag="JN749_001999"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAY7368423.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            21096..22355
                     /gene="rhaA"
                     /locus_tag="JN749_002000"
     CDS             21096..22355
                     /gene="rhaA"
                     /locus_tag="JN749_002000"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAY7368424.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            22597..23421
                     /gene="rhaD"
                     /locus_tag="JN749_002001"
     CDS             22597..23421
                     /gene="rhaD"
                     /locus_tag="JN749_002001"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAY7368425.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            23431..23745
                     /gene="rhaM"
                     /locus_tag="JN749_002002"
     CDS             23431..23745
                     /gene="rhaM"
                     /locus_tag="JN749_002002"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAY7368426.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            24046..>24112
                     /locus_tag="JN749_002003"
     CDS             24046..>24112
                     /locus_tag="JN749_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAY7368427.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT         5985 a         6295 c         6131 g         5701 t
ORIGIN      
        1 tacgaaccag cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa
       61 cgccattgtc tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg
      121 tttagaagat tatatcagca gcaataccgg tctggtgatt gacccgtact tttctggcac
      181 caaagtgaag tggatcctcg accatgtgga aggctctcgc gagcgtgcac gtcgtggtga
      241 attgctgttt ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca
      301 tgtgaccgat tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg
      361 ggacgacaaa atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg
      421 ttcttccgaa gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat
      481 ctccgggatc gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg
      541 gatggcgaag aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc
      601 ggtgaaatca gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa
      661 ctatgcgttg gaaggtgcgg tgtttatggc aggcacatcc attcagtggc tgcgcgatga
      721 gatgaagttg attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac
      781 caatggtgtg tatgtggttc cggcatttac cgggctgagt gcgccgtact gggacccgta
      841 tgcgcgcggg gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc
      901 gacgctggag tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc
      961 tggtatccgt ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat
     1021 gcagttccag tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac
     1081 cgcattgggt gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga
     1141 gctgcaagag aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg
     1201 taattaccgt tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca
     1261 cgacgagtaa tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct
     1321 tacgcgtctt atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca
     1381 cgccgcatcc ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa
     1441 taatcccacc cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta
     1501 tgagacgaga acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct
     1561 acaaatggtt aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc
     1621 gtattatgct caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg
     1681 acgaagcacc gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag
     1741 atattgctgt tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc
     1801 tggcggtgct ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg
     1861 agaagctgat tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg
     1921 ataacctgcg caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga
     1981 ttctggctaa accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg
     2041 tatttgctat tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg
     2101 aagttgacgt gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga
     2161 tccgcgcatt agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg
     2221 acaacgaaga gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca
     2281 tcgaagccgg taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct
     2341 ctgccaccgg tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg
     2401 cgactaccga aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca
     2461 tccactatct gaatcgcaaa gacccggaaa tgcaagtgca cattctctga gtgatttgat
     2521 cgattgagcc ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg
     2581 caagtcaatc aaaacaggag aaaaaaatgg ctgattgggt aacaggcaaa gtcactaaag
     2641 tgcagaactg gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta
     2701 ccgccgggca atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct
     2761 actcctatgt taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg
     2821 atggcaaatt aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta
     2881 gcgaagcagc tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc
     2941 tggcaaccgg tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag
     3001 atcgcttcaa aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt
     3061 tgccactgat gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg
     3121 tcagtcggga aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg
     3181 gggaactgga aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt
     3241 gcggcaatcc acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga
     3301 cgaaacattt acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta
     3361 cttatcgata aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata
     3421 tgccggcatc aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg
     3481 ctctttttgc ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt
     3541 tgtcttcgga cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctc
     3601 caacaattga agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa
     3661 gccatcatca atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc
     3721 tttattcacc agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata
     3781 agccatgttt aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca
     3841 aaaataatca tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc
     3901 tccagatcct taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg
     3961 gcaagctctt tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc
     4021 gaaaacttac tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct
     4081 ttagtactgc atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg
     4141 tcgattacga cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat
     4201 ggattaccat tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat
     4261 tgtcatttgc atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa
     4321 aggttaatta cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata
     4381 tttattttcg ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat
     4441 caacgtcggg acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt
     4501 acccgccagc agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg
     4561 atcatgcaag cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa
     4621 cgccgcggtc tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc
     4681 tgcgaaccac aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga
     4741 gaataaccat tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta
     4801 cccttttgac aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact
     4861 gtcaatgaag ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac
     4921 aacggcgcat gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc
     4981 ccctactttc gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag
     5041 cctgccactg aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg
     5101 tgctgcaagt aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt
     5161 aaaaatcaaa agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc
     5221 gaaagcgaaa gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac
     5281 caaaacacaa agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta
     5341 ttacaccgag acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct
     5401 gaccttcgct gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa
     5461 atgacaaaaa gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc
     5521 cctgcggggc ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat
     5581 cctttagtga tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt
     5641 tctaacctgc gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg
     5701 gaaatgtaca tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg
     5761 caaaacgtgg acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg
     5821 aaagacatcg gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa
     5881 gagtctgacg aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg
     5941 gttctgtgca tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc
     6001 gcacgtcaga tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt
     6061 atcgcttatg aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca
     6121 caggctgttc acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa
     6181 caagtgatca ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct
     6241 cagccagaca tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca
     6301 gtgatcgtta aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc
     6361 atatccggca cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc
     6421 ctggatttcc tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa
     6481 cacaaacgaa ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg
     6541 cgcctcaggc acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc
     6601 cgttacccga cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg
     6661 gctgctgatg ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga
     6721 gatatgaata tgaaaatggt tttgcgtacg cccggtgcgg gagttgatcg ccaaagaaac
     6781 cgcgcgatcg ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc
     6841 caaccaaaag aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat
     6901 acgatacgtt gacatcaaca gatattgcag tggaccatta agatctttta aaaccacgta
     6961 tccggcattg ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg
     7021 caaacattcc tcaaggacac tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca
     7081 gtaaccaata ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc
     7141 ttttttcatc tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac
     7201 aaataaaaaa cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc
     7261 gtttgctgat ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc
     7321 agccgccgaa ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact
     7381 ttttcgctac ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt
     7441 ctggcgagta gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga
     7501 ccactttatc gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga
     7561 tttcgaattt atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga
     7621 tcagtacatc gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat
     7681 ccagaacttc gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt
     7741 tgttgttgtg atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag
     7801 agcttttcgg attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat
     7861 ggatctgctt cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg
     7921 agttatccgg cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt
     7981 ccacgtcata ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg
     8041 tcgcttgttt acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct
     8101 gtttccagtg ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat
     8161 atgaaacgtt aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg
     8221 ttaaccctac gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa
     8281 agcgtgccag aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg
     8341 gtatatgttg agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg
     8401 ctttgtccat gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc
     8461 aataaattgc agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct
     8521 gtcatcggtt tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc
     8581 gcgcagtcca gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg
     8641 tcgtggtgaa ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc
     8701 agcagatcga tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga
     8761 gaaccaccgc gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc
     8821 tcgatgaaat gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg
     8881 tgttttttac ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat
     8941 tcaacttccg gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga
     9001 tcgccacaat aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg
     9061 tcacgcagac ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg
     9121 tagtcagtgc ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc
     9181 atttcggtca gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg
     9241 tcgataccac gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat
     9301 tccgggaaac gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg
     9361 taacggtcta gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc
     9421 attacttcca gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg
     9481 cctggcgcat caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa
     9541 ctttggaatg caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa
     9601 cgaatgtgca aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg
     9661 gcggtatttt atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata
     9721 gaccgtttac cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat
     9781 cccggaaaac gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc
     9841 aaaggcagag agtcttccat ttccaaatgg atctgaataa agcgggtcgg ccctgactgc
     9901 cgcgtgcgaa gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt
     9961 tcttgccgtt cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc
    10021 atgcgtaacg cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg
    10081 cgatgccagc cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca
    10141 tcagactggt aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc
    10201 caccgctgaa acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc
    10261 ccgacgcctg gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac
    10321 aggaatagtg ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct
    10381 ttaccgtgac caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt
    10441 aaattcgtca acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc
    10501 accgacccgg tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc
    10561 gtcgcagcaa tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc
    10621 gctatcaact gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc
    10681 aaattgagga taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc
    10741 aaggaaaaca gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat
    10801 gaactttttt gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg
    10861 tttaaaaccg cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc
    10921 atctcaacct gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg
    10981 ttttcatcaa aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta
    11041 acaggaggct gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt
    11101 tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca
    11161 cccggatgcc agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc
    11221 agcgttgagg ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct
    11281 gtcgcgattc aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta
    11341 aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat
    11401 ttaaacaatg aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa
    11461 ggagctgctc gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct
    11521 tgatcttctg gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa
    11581 tggtatcgac acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac
    11641 ggcgcgcggc agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct
    11701 cccgaaaccg tttaatgatc gtgagctggt ggcacgtatt cgcgcgatcc tgcgccgttc
    11761 gcactggagc gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc
    11821 cttagtgctg aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac
    11881 cggtactgag tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg
    11941 tgaacattta agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga
    12001 tatgcacatt tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt
    12061 taaaaccttg cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc
    12121 gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg tgttgatgtt ggttttgatg
    12181 ttacccaagc tcgattcacg ccagatgacc aagcttctgg atagcgaaca gcgtcagggg
    12241 ctgatgattg agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg
    12301 tggtggcggc gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta
    12361 ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt
    12421 aactttattg gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg
    12481 gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt
    12541 cgtccggcca gcagttctca atccgatttc attaacttac tgtttgaccg cccgctatta
    12601 ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg
    12661 gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc
    12721 cagcacccgg aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac
    12781 cagatggtca ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc
    12841 tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt
    12901 cgtagcggtg aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc
    12961 atgatcaacg atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa
    13021 accatcaaag ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag
    13081 caaatgggca agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat
    13141 ccgaacgccc tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat
    13201 acgaagattg aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac
    13261 gatggtcctg gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc
    13321 gatgaagcac gcgatcgtga atctggcggt acaggattgg ggctggcaat tgttgaaacc
    13381 gccattcagc agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg
    13441 ctggtgattt ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa
    13501 ggcgtttacg ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt
    13561 atcaggccta cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt
    13621 gccgctcagt cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga
    13681 gagtaaacgg tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc
    13741 ctgcacggtc acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt
    13801 ccctggcgct atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc
    13861 tatatcgctg atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac
    13921 ctgaattaat gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag
    13981 accgtcggtt gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg
    14041 aaattcccgc acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg
    14101 cccgccgtga acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat
    14161 caactcacca tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg
    14221 aatcttgcct gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt
    14281 ccccattaga ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc
    14341 aggaactcaa tagcaaaccg gacgaacgtt agttcatccg gctgtgggga ttaaggaagc
    14401 aggtttattt caccgtacct aaaatttcga ttttcgctgc tctggctttg actttacgtg
    14461 accagataga ggtaagaatt ggcaccagaa tcgaagtcac aatgaccgcc gtcgctacca
    14521 gcgaagttgc tgccggagcc atcggtttaa acgcaggcac catttctgca atcagcacag
    14581 gtgtcgctac cgcggcccct gcggaactgg aagcggcaat tccggcagtg ccatcgccac
    14641 cgccaatcaa tttatcggcg ataatcaacg gaataccggt cacgataatt attgccacac
    14701 ccaacaggat ccccagcaaa ccagtctggg caatcacagt caaatcaatg gtattgccca
    14761 gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt gctgaaaaat tctcgtaatt
    14821 cagggtcaag attccccagg gcaaagccca ccaggaacgg taatactgcg ccgacgaaaa
    14881 catgcggttc aaacgaggca atcccggcag tgcccagaat aatcatcgtc atgagcggcc
    14941 cggactccaa cgacatcaac acaaatgccc cagcttcttc tttcgtaccg tactgctgca
    15001 tgatggaagc gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg
    15061 aaagtccggc aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg
    15121 ccgcaaccac ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta
    15181 ccgttcccgt cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa
    15241 tgggcaccgt accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg
    15301 gcgagaaggt gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga
    15361 ttttctcaat cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct
    15421 gatgcgtaat tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta
    15481 atcaataaaa gtgatcaaaa ccacatcaat tgaaacgctg ttttattttt ataggatcat
    15541 tgtttttatt ttggaattgt tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt
    15601 aagctgatat gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc
    15661 gcaaaacgtg ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc
    15721 ggacggcggt tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc
    15781 gggaagccgg aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta
    15841 gaaaccaccg ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg
    15901 gaagctgccg ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg
    15961 atagccgctt tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg
    16021 ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc
    16081 tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc
    16141 agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg
    16201 gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta
    16261 tagctcatat tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt
    16321 ggtttattat agttaattaa atgatattga aaatgattat caatgccgca cttttcgtaa
    16381 gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg
    16441 attgccagta aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt
    16501 acactcatct cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa
    16561 cgcgattacg atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta
    16621 cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat
    16681 tgtttcgtgg attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc
    16741 gtattacagc tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg
    16801 ggggatcggt aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat
    16861 tggcatcgcc attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg
    16921 caatttcgat gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt
    16981 ggcgctgatt ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg
    17041 cattaaagcc gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat
    17101 tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc
    17161 cgcacttggc gtcgatccac tgtatgtcgc tctgccaagc tatgttgtca tcatgggcgg
    17221 cggcgcgatc attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc
    17281 gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc
    17341 actgggcggg ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat
    17401 tccggcgcag tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg
    17461 cggtatcgtc gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt
    17521 gttgagcctc ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc
    17581 gaattaatct ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg
    17641 ggtaaacacc accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact
    17701 gattaacagg cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag
    17761 atattgattg atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact
    17821 gcacgatgcc tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag
    17881 ccgggtaatc agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt
    17941 gtaacgatgg cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt
    18001 agcaaacggc acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg
    18061 cgccatcccc acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg
    18121 aatcgctccc tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg
    18181 caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag
    18241 atcgccacgg gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca
    18301 gacaatcacc agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg
    18361 gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa
    18421 ctgatgcgcc accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg
    18481 tacaaatacg ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat
    18541 cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga
    18601 atccacagcg ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcggg
    18661 ctttcatcag tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa
    18721 gctgccgatg tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca
    18781 cctcatcggc aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt
    18841 tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga
    18901 tctcgcgact ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa
    18961 ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc
    19021 catccagctc ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg
    19081 agcgatacag cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat
    19141 gatcgcgtac gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat
    19201 gaatacccgt gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa
    19261 aatccgcctg cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat
    19321 ccacactacg taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa
    19381 tcacgaggtc aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt
    19441 tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag
    19501 caaattgtga acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc
    19561 agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag
    19621 gatcacatta tgacctttcg caattgtgtc gccgtagatc tcggcgcatc cagtgggcgc
    19681 gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt
    19741 tttaacaatg ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa
    19801 agtgccattc gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt
    19861 gggattgata cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc
    19921 ctgcccgttg cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa
    19981 ctcggcaaac gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt
    20041 tatcagttgc gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct
    20101 ctgctgatgc cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc
    20161 aatgccacca ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg
    20221 gcgtggagcg gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt
    20281 gggcactgga tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat
    20341 accgccagcg cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct
    20401 ggcacctggt cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg
    20461 gcagccaaca tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt
    20521 atgggcttat ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg
    20581 cttatcgccg cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat
    20641 cgctttatta atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg
    20701 caaccgatcc cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg
    20761 ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg
    20821 catattgtcg gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc
    20881 ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag
    20941 ttaattacgc tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc
    21001 gcgaatctga ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag
    21061 attcactcta cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct
    21121 gggagctggc gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc
    21181 aacttgatcg tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg
    21241 aaaacccgga aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag
    21301 cgcgtaatgc cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc
    21361 cgaaacggct taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc
    21421 agatcaaacc agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc
    21481 tggattttaa cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc
    21541 atcccgacga cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt
    21601 cggcctattt tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg
    21661 gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg
    21721 atgaggtgat cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat
    21781 tgtttggcat tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg
    21841 ccaccagccg ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga
    21901 tttccgacaa gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc
    21961 gtccggttcg ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg
    22021 ccagtgagat tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg
    22081 atgcctctat caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc
    22141 tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt
    22201 acactgcgcg tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg
    22261 aaatgtattg ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg
    22321 cttatgagaa agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacaagcgc
    22381 cttatccggc ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc
    22441 ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg
    22501 gacagtcccc tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt
    22561 ctaaacaagg caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg
    22621 tttgtccagg gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc
    22681 aacggcggca acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac
    22741 ttccacccac aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg
    22801 ccgtttattg tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct
    22861 aacttaggcg tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta
    22921 accaacgaag ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc
    22981 attaaagcca ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc
    23041 gccctcacct atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc
    23101 agcaccgaat gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc
    23161 ggcacggacg aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg
    23221 tggcccttcc acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc
    23281 gacaccgcag aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa
    23341 cagaccatca gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta
    23401 gccagtgcgc tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta
    23461 aaccccgacg cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa
    23521 gcagtgttga aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat
    23581 ctgctgtttg ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact
    23641 gatgtttgcc aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac
    23701 agcccggtga gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg
    23761 gagatgcctc cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc
    23821 ccggtaagtt atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca
    23881 ttaatgattc atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc
    23941 tttcactctg tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc
    24001 tccaccatta caattgatta aaatatattt tatggagtcc gattaatggc agctcttact
    24061 gcaagctgta ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DADLTF010000054        23922 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.054, whole genome shotgun sequence.
ACCESSION   DADLTF010000054 DADLTF010000000
VERSION     DADLTF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.054"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            10..333
                     /locus_tag="JN749_002004"
     CDS             10..333
                     /locus_tag="JN749_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005072913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7368428.1"
                     /translation="MPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDA
                     GVWFKKAAAQGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNEL
                     EHQTK"
     gene            386..2116
                     /locus_tag="JN749_002005"
     CDS             386..2116
                     /locus_tag="JN749_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368429.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWMKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(2176..2667)
                     /gene="yajQ"
                     /locus_tag="JN749_002006"
     CDS             complement(2176..2667)
                     /gene="yajQ"
                     /locus_tag="JN749_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HAY7368430.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2835..3746
                     /gene="panE"
                     /locus_tag="JN749_002007"
     CDS             2835..3746
                     /gene="panE"
                     /locus_tag="JN749_002007"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAY7368431.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3709..4299
                     /gene="yajL"
                     /locus_tag="JN749_002008"
     CDS             3709..4299
                     /gene="yajL"
                     /locus_tag="JN749_002008"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HAY7368432.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(4353..5801)
                     /gene="thiI"
                     /locus_tag="JN749_002009"
     CDS             complement(4353..5801)
                     /gene="thiI"
                     /locus_tag="JN749_002009"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAY7368433.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            6007..6249
                     /gene="xseB"
                     /locus_tag="JN749_002010"
     CDS             6007..6249
                     /gene="xseB"
                     /locus_tag="JN749_002010"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAY7368434.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            6249..7148
                     /gene="ispA"
                     /locus_tag="JN749_002011"
     CDS             6249..7148
                     /gene="ispA"
                     /locus_tag="JN749_002011"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HAY7368435.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            7173..9035
                     /gene="dxs"
                     /locus_tag="JN749_002012"
     CDS             7173..9035
                     /gene="dxs"
                     /locus_tag="JN749_002012"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAY7368436.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            9090..10064
                     /gene="yajO"
                     /locus_tag="JN749_002013"
     CDS             9090..10064
                     /gene="yajO"
                     /locus_tag="JN749_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HAY7368437.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEENSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(10118..10636)
                     /gene="pgpA"
                     /locus_tag="JN749_002014"
     CDS             complement(10118..10636)
                     /gene="pgpA"
                     /locus_tag="JN749_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAY7368438.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10614..11591)
                     /gene="thiL"
                     /locus_tag="JN749_002015"
     CDS             complement(10614..11591)
                     /gene="thiL"
                     /locus_tag="JN749_002015"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HAY7368439.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDVLSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11669..12088)
                     /gene="nusB"
                     /locus_tag="JN749_002016"
     CDS             complement(11669..12088)
                     /gene="nusB"
                     /locus_tag="JN749_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAY7368440.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(12108..12578)
                     /gene="ribE"
                     /locus_tag="JN749_002017"
     CDS             complement(12108..12578)
                     /gene="ribE"
                     /locus_tag="JN749_002017"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HAY7368441.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGSAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12667..13770)
                     /gene="ribD"
                     /locus_tag="JN749_002018"
     CDS             complement(12667..13770)
                     /gene="ribD"
                     /locus_tag="JN749_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HAY7368442.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13774..14223)
                     /gene="nrdR"
                     /locus_tag="JN749_002019"
     CDS             complement(13774..14223)
                     /gene="nrdR"
                     /locus_tag="JN749_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HAY7368443.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14374..14913
                     /locus_tag="JN749_002020"
     CDS             14374..14913
                     /locus_tag="JN749_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HAY7368444.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            15212..16096
                     /locus_tag="JN749_002021"
     CDS             15212..16096
                     /locus_tag="JN749_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HAY7368445.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16273..16620)
                     /locus_tag="JN749_002022"
     CDS             complement(16273..16620)
                     /locus_tag="JN749_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HAY7368446.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16749..17720)
                     /gene="secF"
                     /locus_tag="JN749_002023"
     CDS             complement(16749..17720)
                     /gene="secF"
                     /locus_tag="JN749_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HAY7368447.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17731..19578)
                     /gene="secD"
                     /locus_tag="JN749_002024"
     CDS             complement(17731..19578)
                     /gene="secD"
                     /locus_tag="JN749_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HAY7368448.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19606..19938)
                     /gene="yajC"
                     /locus_tag="JN749_002025"
     CDS             complement(19606..19938)
                     /gene="yajC"
                     /locus_tag="JN749_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAY7368449.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19961..21088)
                     /gene="tgt"
                     /locus_tag="JN749_002026"
     CDS             complement(19961..21088)
                     /gene="tgt"
                     /locus_tag="JN749_002026"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAY7368450.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDMHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(21143..22213)
                     /gene="queA"
                     /locus_tag="JN749_002027"
     CDS             complement(21143..22213)
                     /gene="queA"
                     /locus_tag="JN749_002027"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAY7368451.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22306..22879
                     /gene="acpH"
                     /locus_tag="JN749_002028"
                     /pseudo
     CDS             22306..22879
                     /gene="acpH"
                     /locus_tag="JN749_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(22884..>23922)
                     /gene="malZ"
                     /locus_tag="JN749_002029"
     CDS             complement(22884..>23922)
                     /gene="malZ"
                     /locus_tag="JN749_002029"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7368452.1"
                     /translation="DRHNLGTGGACHNSESPWRDWYLFSDDGTALDWLGYASLPKLDY
                     QSESLVNEIYRGEDSIVRHWLKAPWNMDGWRLDVVHMLGEAGGARNNMQHVAGITEAA
                     KETQSEAYIVGEHFGDARQWLQADVEDAAMNYRGFTFPLWGFLANTDISYDPQQIDAQ
                     TCMAWMDNYRAGLSHQQQLRMFNQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVP
                     CIYYGDEVGLDGKNDPSCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVL
                     YAEDNVVVFVRVLNQQRVLVAINRGEASEVVLPASPFLNAVQWQCKEGHGQLTDGILA
                     LPAISATVWMN"
BASE COUNT         6079 a         6270 c         5879 g         5694 t
ORIGIN      
        1 ggcaatggta tacctgcaaa caatgaaaaa tacctagcat atctgcaaca agctgcaaca
       61 ctgggcagta gtaatgccca ggcggaaatt ggctatttat atctcatcgg aaaagaacta
      121 ccacagaatc ttcctgatgc tggtgtctgg ttcaaaaaag cagctgcaca aggtaatttt
      181 gccgcacatt tctatctggg aaggatgtat cagaatggtg acggcgttga gagaaatatg
      241 gaaaaagcca ggtttcatct atctaatgcg gcagaaggtg gaatagaacc tgcgcttaaa
      301 gcattaaatg aacttgaaca tcaaacaaaa taaaacgctc agggaccacg taaggttcat
      361 caaacagaaa aggatatagt ttgcgatgaa agcgaaatct actttgttat taatgatttt
      421 atctgttttt tattctcatc aggttttatc cagcgacccc actcagccgg atgtttattc
      481 tgtcgtcgag aaaaaactaa actctgcttt acctctggaa gaaaatacca ccttcagatc
      541 gcaggctgaa tggtttaatt tgcaatatga attattagta tctggctacc ctgaacgtgc
      601 ttacaattta ctgagcaaac ttgagacaga atcgaaaaac gctaaatatt atctggattt
      661 aactgcctcc agagtcgctg aagaagaagc accagagaca gccttaactt ttttatcaaa
      721 aattgggtta aataaaccgg ctactcttac atcctttgag agctatatca atagctggat
      781 gaaaagcaaa caacccgaaa aggctatgca gcttctcagc ctcgatgaga atgccctgaa
      841 ttattattta ccgactgtgt tagtcgcata tcagatcgtt cctgataagg caataagtat
      901 ttataattct gtatatggag ataatgttgt tataccctat cagcaagtca ccatgttact
      961 tacagttgcg gagggttatc aaacaaaagg cgatataaaa aacgccattc attatgccga
     1021 taaggcgctg aatatgtttg ataaagctat tgctgtagat agcagtcgtg aatcccatta
     1081 tagtgatgag tatttgaaac tcatgaatat ctacgccgcc aatggcaaca aagaaaaagc
     1141 ggtggtttta tctcaacgtc tacacaaagc cattaccgaa tctcaatcga attacttaag
     1201 tactctggaa ggaattttat tgttttatag aaataatgat atgcagcaag actatcaaaa
     1261 cagcctttca aattacatag tatttatcga tgaaattttt gccttttccc catcaacaag
     1321 aagtgaatta agcctcatta atttattaaa ttcattaaat gaggtggaat tgatgagaaa
     1381 acgtctggcg tttctgatga cagcaccaga atacgcttgt tatgatagtc agtattgtta
     1441 tgaagacaaa gtcagcgcat taaaaatcct gtatcgtcat catgacaatg agctaatgga
     1501 caaatatttc acgactcttc ttaaagagat cagtaatcaa ccattagaag actgggatat
     1561 aataataagt aatactggtg aaaaatttag tgaagtgggt ctaaccaaat atgctcaaca
     1621 tctcgcatct ttcgctgaag aattttatat aaaagagcaa aaatcacatt ctgaaagtga
     1681 aatgagagga ctattttcat cacttgccga attatatagt tttggtaatg atactgccag
     1741 cgcaaaccga gtgttccatc agcatgtccc tgccttcaat acagatcata tgattcaata
     1801 ttttataaat gccaaagaat ggaacaatgc cagggaatta ttgataaaag aggaaatgtt
     1861 gggtatgcat aatctgatat tgttggagaa tatttgtatg caaaaaaatg cagaatgcat
     1921 ggcacacatt acctttacgc ttaataaatt aacaacacaa ccagcaataa caaaaataga
     1981 tagcgtgggt aatgagcaac tttatcagat tggcaaaatc taccataagt tggaaattaa
     2041 acctgagcca gagcaacagg ttttaattca gtcgctttat gaccgggcat caggaagcgc
     2101 ctctgcaact caataaatgc atggtcaaaa gggccaccac ggtggccctg acaatcagta
     2161 gctatatcca gaaagttaat cgcggaagtt tttgaactgg aacggctgac cgagatcgcc
     2221 accgcgcacc atcgccatga cagcctgcaa atcatcacga gatttgccag tcacgcggat
     2281 ctcatcgccc tgaatttgcg cctgcacttt cagtttgctg tctttgatca tcttgacgat
     2341 ttttttctga gtggcgctct caatgccctg tttcagtttc gcttccacaa accaggtttt
     2401 accgctatga acgatatttt ccggtacatc cagtgaactg ccttcaatgc cgcgcttcag
     2461 cagcttggca cgcagaatat ccagcaactg attgacctgg aagtcggact cgctcaacac
     2521 tttgatggtt ttgctggcgt cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa
     2581 acgggactcc acttcgcgac tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac
     2641 ttcagagaca atatcgaaag atggcatctt ttcctctccc ttcatttttg atgcgaagca
     2701 taatacctgc aaagttaaat aaccaacagt cctgcaccag gacgtatccc ttaattgtgc
     2761 atagtcgcaa cagcatgcac aattaaggga tagccttatt gaacacccgg agtggttgtg
     2821 ggtgaggagg aacaatgaaa attaccgtat tgggatgcgg tgccttaggg caattatggc
     2881 ttacagcact ttgtaaacag ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt
     2941 attgtagtgt gaatctggtt gagacagatg gttcgatatt taacgaatcg ctgaccgcca
     3001 acgatcccga ttttctcgcc accagcgatc tgctcctggt gacgctgaaa gcatggcagg
     3061 tttccgatgc cgtcaaaagc ctcgcatcca cactgcctgt aactacgcca atactgttaa
     3121 ttcacaacgg catgggcacc atcgaagaat tgcaaaacat tcagcagcca ttactgatgg
     3181 gcaccaccac ccatgccgcc cgccgcgacg gcaatgtcat tattcatgtg gcaaacggaa
     3241 tcacgcatat cggtccggca cggcaacagg atggcgatta cagttatctg gcggatattt
     3301 tgcaaaccgt gttgcctgac gttgcctggc ataacaatat tcgcgccgag ctgtggcgca
     3361 agctggccgt caactgcgtg attaatccac tgactgccat ctggaattgc ccgaacggtg
     3421 aattacgtca tcatccgcaa gaaattatgc aaatatgcga agaagtcgcg gcagtgatcg
     3481 aacgcgaagg gcatcatact tcagcggaag atttgcgtga ttacgtgatg caggtgattg
     3541 atgccacagc ggaaaatatc tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg
     3601 aaatcgacta tatcaatggt tttctcttac gccgcgcccg cgcgcatggg attgccgtac
     3661 cggaaaacac ccgcctgttt gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg
     3721 gcactggttt gcctcgcccc tggtagtgaa gagactgaag ccgtcaccac gatcgacctc
     3781 ttggttcgcg gcggtatcaa agtcaccact gccagcgtcg acagcgatgg caacctggca
     3841 attacctgct cgcgcggcgt gaagctgctg gcggatgcgc cgctggtcga agtggctgat
     3901 ggcgaatatg acgtgatcgt gctgcctggt ggcattaaag gcacggagtg ttttcgcgat
     3961 agcactctgc tggttgaaac cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct
     4021 atttgcgccg cgccagccac cgtgctggtg ccgcacgata tctttccgat tggcaatatg
     4081 accggcttcc cgacgctgaa agacaaaatt cccgctgaac aatggcagga caagcgcgtc
     4141 gtctgggatg cacgggtaaa attgctgacc agccaggggc cgggtacagc tatcgacttt
     4201 ggtctgaaaa ttatcgacct gctggttggg cgcgagaaag cccatgaagt ggcatcacaa
     4261 ctggtgatgg cggcagggat ttataattat tacgagtagt gtcggatgcg gcaaacgtcg
     4321 catctgacca gatgcgacgt aaaaactaca aattacgggc gatatacctt cacattgtta
     4381 aagccttgct cgcgcagata gagcgcctgc agacggctca tcaccccgcg ctcacaccac
     4441 agcagccagg ttttgttctg gtcgagatcg ccaaatttgg tgctcagttt atagaacggc
     4501 agagaaacca catcaatccc ttcgactttc agtggcttat cttcctgttc atcgacagaa
     4561 cggatatcga gaatcacgtc gttcgggccg aagccgttga cggtttccac ttccaccact
     4621 tcctgctcgg tctgctgggc gatttcgcgg atatcaacgt tattcgcttc ctcaaccact
     4681 ttatcgagaa tactgaagtc gaacttctct tcttccgctt caatcttcga tttaaccgct
     4741 ttcaccgtcg ggcttttgga gatcacgcca caatattccg gcatcgtgcg agcaaagtct
     4801 tcggtgccaa tctggcgagc caggttgatg atgtgctctt tgtcgtaaga aatcagcgga
     4861 cgcaggatca gcgtgtcaga gacgttatca atcaggcgca ggttggtcag cgtctgactg
     4921 gacacctggc cgagcgcttc gccggtgacc agcgcctgta cgccgtaacg ttcagccact
     4981 ttagatgcgg cacgcaccat catacgtttg aggataacgc ccatctgacc gtcgtcgatt
     5041 ttctcgagaa tttccccgac aaccggttcg aaattaatgg cgacaaaacg cacgcggtgg
     5101 gagctgccaa agcggttcca cagataatgc gccacctgac gcacgccaat ttcatgcgct
     5161 gcgccgccga ggttaaagaa gcagtaatgc acgcggcagc cgcgacgcat caacatataa
     5221 ctggaaacac cggagtcgaa accaccggaa atgagcgaca gcacatcttc ctgggtgcca
     5281 atcgggaaac caccaatacc ttcgtagcga cctttaatca gcaggaggcg atcgtcttcc
     5341 acttccagat gaaccgttac atccgggtta gtcagcttca cgcgcgcgga ttcaatatgc
     5401 tgatttaaac cgccgccgac gtaacgttcc acatcaatcg agctaaaatc atgtttgcca
     5461 cggcgcttca cgcgtacgca gaaggttttg ccttccagct gatcgcgata ctgaaccaac
     5521 gctttctcaa aaatatcgtg catgtcggta aacggcacgt cttcgacttc gagaatatgg
     5581 tggatacccg gaatacgggt cagggcgtcg cgaatagtca gacgctggtt ttcatctttc
     5641 gcgcgaactt cgatgttatc ccagtggcgg acgacagcga gcgtctcatc atagtgcttt
     5701 aaaacgttac gaatgttccc ggtaaggatt tttataaagc gcaagcgcac agattggctt
     5761 ttgatggtga tttccgggaa caatttaatg ataaacttca tggcggcaat ggttcgttgg
     5821 caagccttaa gcgacttgta tagggaaaaa tacagcagcc cacacctgcg gctgcatcca
     5881 ggcgcggaag tataccacta acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt
     5941 atttgctatt tgccctgagt ccgttaccat gacggggcga aaaatattga gagtcagaca
     6001 ttcattatgc cgaagaaaaa tgaggcgccc gccagctttg aaaaggcgct gagcgagctg
     6061 gaacagattg taacccgtct ggaaagtggc gacctgccgc tggaagaggc gctgaacgag
     6121 ttcgaacgcg gcgtgcagct ggcacgtcag gggcaggcca aattacaaca agccgaacag
     6181 cgcgtacaaa ttctgctgtc tgacaatgaa gacgcctctc taaccccttt tacaccggac
     6241 aatgagtaat ggactttccg cagcaattcg aagcctgcgt taagcaggcc aaccaggcgc
     6301 tgagccgttt tatcgcccca ctgccctttc agaacactcc cgtggtcgaa accatgcagt
     6361 atggcgcatt attaggtggt aagcgcctgc gacctttcct ggtttatgcc accggtcata
     6421 tgtttggcgt tagcacaaac acgctggacg cacccgctgc tgccgtagag tgtatccacg
     6481 cttactcatt aattcatgat gatttaccgg cgatggatga tgacgatctg cgtcgcggtt
     6541 tgccgacctg ccatgtgaag tttggcgaag caaacgcgat tctcgctggc gacgctttac
     6601 aaacgctggc gttctcgatt ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca
     6661 gaatttcgat gatttctgaa ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc
     6721 aggcattaga tttagacgcg gaaggcaaac acgtacctct ggacgcgctt gagcgtattc
     6781 atcgtcataa aaccggcgca ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg
     6841 gagataaagg gcgtcgtgct ctgccagtac tcgacaagta cgcagagagc atcggccttg
     6901 ccttccaggt tcaggatgac atcctggatg tggtaggaga tactgcaacg ttgggaaaac
     6961 gccagggtgc cgaccagcaa cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc
     7021 aagcccggaa gaaagcccgg gatctgatcg acgatgcccg tcagtcgctg aaacaactgg
     7081 ctgaacagtc actcgatacc tcggcactgg aagcgctagc ggactacatc atccagcgta
     7141 ataaataaac aataagtatt aataggcccc tgatgagttt tgatattgcc aaatacccga
     7201 ccctggcact ggtcgactcc acccaggagt tacgactgtt gccgaaagag agtttaccga
     7261 aactctgcga cgaactgcgc cgctatttac tcgacagcgt gagccgttcc agcgggcact
     7321 tcgcctccgg gctgggcacg gtcgaactga ccgtggcgct gcactatgtc tacaacaccc
     7381 catttgacca attgatttgg gatgtgggac atcaggctta tccgcataaa attttgaccg
     7441 gacgccgcga caaaatcggc accatccgtc agaaaggcgg tctgcacccg ttcccgtggc
     7501 gcggcgaaag cgaatatgac gtattaagcg tcgggcattc atcaacctcc atcagtgccg
     7561 gaattggtat tgcggttgct gccgagaaag aaggcaaaaa tcgccgcacc gtctgtgtga
     7621 ttggcgacgg cgcgattacc gctggcatgg cgtttgaagc gatgaatcac gcgggcgata
     7681 ttcgtcctga tatgctggtg gtcctcaacg acaatgaaat gtcgatttcc gaaaatgttg
     7741 gcgcgcttaa caaccatctg gctcagctgc tttccggtaa gctttactct tcactgcgcg
     7801 aaggcgggaa aaaagttttc tctggcgtgc cgcccattaa agagctgctc aaacgtaccg
     7861 aagaacatat taaaggcatg gtggtgcctg gcacgttgtt tgaagagctg ggatttaact
     7921 acatcggccc ggtggacggt cacgatgtgc tggggcttat caccacgcta aagaacatgc
     7981 gcgacctgaa aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac
     8041 cggcagaaaa agacccgatc actttccacg ccgtgcctaa atttgatccc tccagcggtt
     8101 gtttgccgaa aagtagcggc ggtttgccga gctattcaaa aatctttggc gactggttgt
     8161 gcgaaacggc agcgaaagac aacaagctga tggcgattac tccggcgatg cgtgaaggtt
     8221 ccggcatggt cgagttttca cgtaaattcc cggatcgtta cttcgacgtg gctattgccg
     8281 agcaacacgc agtgaccttt gccgccggtc tggcgattgg tggatacaaa cccattgtcg
     8341 cgatctactc caccttcctg caacgcgcct atgatcaggt gctgcatgac gtggcaattc
     8401 aaaagctacc ggtcctgttc gccattgacc gcgcgggcat tgttggtgct gacggtcaaa
     8461 ctcaccaggg cgcttttgat ctctcttacc tgcgctgcat accagaaatg gtcattatga
     8521 ccccgagcga tgaaaacgaa tgtcgccaga tgctctatac cggctatcac tataacgatg
     8581 gcccgtccgc ggtgcgctac ccgcgcggca acgcggttgg cgtggaactg acgccgctgg
     8641 aaaaactacc aattggcaaa ggcattgtga agcgtcgtgg cgagaaactg gtgatcctta
     8701 actttggtac gctgatgcca gaagcggcca aagtcgccga atcgctgaac gctacgctgg
     8761 tcgatatgcg ttttgtgaaa ccacttgatg aagcgttaat tctggaaatg gccgccagcc
     8821 atgaagcgct ggtcaccgta gaagaaaacg ccattatggg cggcgcaggt agcggagtga
     8881 acgaagtgct gatggcccat cgtaaaccag tacccgtgct gaacattggc ctgccggact
     8941 tctttattcc gcaaggaact caggaagaaa tgcgcgccga actcggcctc gatgccgccg
     9001 gtatggaagc caaaatcaag gcctggctgg cataatccct actccactcc tgctatgctt
     9061 aagaaattat tcatggcagg agtggcagca tgcaatacaa ccccttagga aaaaccgacc
     9121 ttcgcgtttc ccgactttgc ctcggctgta tgacctttgg cgagccagat cgcggtaatc
     9181 acgcatggac actgccggaa gagaacagcc gtcccataat taaacgcgcg ctggaaggtg
     9241 gcataaattt ctttgatacc gccaatagct attccgatgg tagcagcgaa gagatcgttg
     9301 gtcgcgcact gcgggatttc gcccgtcgtg aagacgtagt cgttgccacc aaagtgttcc
     9361 atcgcgttgg tgatttaccg gaaggattat cccgtgcaca aattttgcgc tctatcaacg
     9421 acagcctgcg acgtctcggc atggattatg tcgatatcct gcaaattcat cgcggggatt
     9481 acaacacgcc gatcgaagag acgctggaag ccctgaacga cgtggtaaaa gccgggaaag
     9541 cgcgttatat cggcgcgtca tccatgcacg cttcgcagtt tgctcaggcg ctggaactac
     9601 aaaaacagca ctgctgggcg cagtttgtca gtatgcagga tcactataat ctgatttatc
     9661 gcgaagaaga gcgcgagatg ctgcctctgt gttatcagga gggcgtggcg gtgattccgt
     9721 ggagcccgat ggcgcggggg cgactgacgc gtccatgggg agaaactacc gcacgactgg
     9781 tgtctgatga ggtggggaaa aatctctata aagaaagcga tgaaaatgac gcgcagatcg
     9841 cagagcggtt aacgggcgtc agtgaagaac tcggtgcaac acgagcacaa gttgcgctgg
     9901 cctggttgtt aagtaaaccg ggcattgccg caccgattat cggaacttcg cgggaagaac
     9961 agcttgatga gctgctgaac gcggtggata tcactttgaa gccagaacag attgccgagc
    10021 tggaaacgcc gtataaaccg catgcggtcg taggatttaa ataagagtcc aggcctgatg
    10081 agacgtgaca agcgtcacat caggcatcgg tgcacaacta cgacagaata cccagcggcc
    10141 agtgatgacc gataaagtac aggatgcctg cggaaatcac cccggcgaca atatcgtcga
    10201 tcatgatccc catgccgcca tgtacattgc gatcaaacca gcggatcggc cacggcttcc
    10261 acatatccag aatacggaaa atcacaaacc cggcggcaac ccactgccag tcattggtcg
    10321 gcagcgccat gagcgtgatc cacataccaa taaattcgtc ccagacaatg ctgccgtgat
    10381 cgtgcacgcc catatctttc gccgtttgat gacaaagata gacgccgata cagatcccca
    10441 acatcaccac cagcgagtag agctgccagg gtaaaaaggt catcagatac cagaaaggga
    10501 tcgccgccag cgatcccatc gtgccaggaa cgattgggct taatccactt ccgaatccga
    10561 cagcaagcag atgccacgga ttactcatct tcaggcgact tttcgcgaca tctttatggc
    10621 gtggcaaaat ggtcatatcc tttccagtca aacgtgacag gttcgccgtc acgaataaaa
    10681 caaagccctt cgatatcggc ggtcatttgc ccgatacagg taaacggtac gccaaggtgt
    10741 ccgagagcaa catccagcgc gccacggttc agttccggca ccgtgaaaca caactcgtaa
    10801 tcttcaccgc cagagagcgc ccagcgcagc gcctgttccg gttcaacatg gcgagaaagc
    10861 acatcagaaa acggcagcaa tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg
    10921 atatgcccga gatcggaaat cagaccgtca gagagatcga tagctgaatt tgccagatcg
    10981 cgcagtgcct gcccctgtaa aatacgcggc gatggacgga gatgacgttt gatcaagtag
    11041 tcagcatctt tagcatcggc aacctgcaaa cggttttgca aaatcgccag cccggcggcg
    11101 ctatcgcccg gtgtaccggt cacatagatc cagtcacccg gtttcgcccc agagcgcgtt
    11161 aaggctcgtc ccatcggaac aaagccgtgg atacccaacg tcattgataa tggcccacgc
    11221 gtggtatcgc cgccaatgag ttgcatatcg taataattga gaagatcaaa caaactgtcg
    11281 ctgaaggact caagccacgc ttcgtctacg tccggtaagg ttaatgccag cgtcagccag
    11341 gccggatcgg cccccatcgc tgccagatcg cttaggttca ccgccagtgc tttataagcc
    11401 agatcagcag gatcgatatc agggaggaaa tggttgcccg ccaccagcgt atcagtgctg
    11461 atcgccaggg tctgtttctc ggggatattg agaagagcgc aatcgtcgcc gatgcccagt
    11521 tcgacatcaa gacgagaact tcttacacgg tcaaaataac gggcaatcag ggagaactcg
    11581 ccacatgcca tacgttatgc ctcagcaggt aaaaaagaaa aggccggcga tcatggaaca
    11641 gtcttccgtg aatctaccgg cctggatatt actttttgtt agggcgaatc acaggtgctg
    11701 ctttatcgag tacgccgttg acgaacttat ggctgtcttc tgcgccgaac gatttcgcca
    11761 gttcgatcgc ttcgttaatg gccactttgt atggcacatc actacgttta gacagttcgt
    11821 acagcgcaat gcgcagtact gctttttcta cctggcccag ttcttccagc aggcgggaca
    11881 ggtagggctt catcagtccg tcgaggtatg cggtgttagt cgccaccccg gccagcagct
    11941 cacggaagta cagaacgtca acgtctttta catcctgttc agccaggaac tggtattcaa
    12001 catcagcgat gtcgttctgg gacaactgcc aggagtagag cgcctggacg gcacactcac
    12061 gagcgcggcg acgagcagca ggtttcacgg atttcccctt actaatttca ggccttgatg
    12121 gctttcaata cattaatcat ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca
    12181 gctttggtgc cagcacgttc gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa
    12241 gcaaccggaa tttcgctgtc ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg
    12301 acatattcaa agtgggcaga gccaccacga ataaccgtac ccagcgcaat caccgcgtcg
    12361 tatttaccgg ttttagccag tgcacccgcc gccagcggca gctcataggc accaggcacc
    12421 caaacaacgg taatgttttc atcttttacc tgaccgatac gtttcagtgc gtcaattgca
    12481 ccttccagca ggctgtcatt gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga
    12541 gcgtccgggg tagcaacgtt agcttcaata atgttcattt tcttccttcg ggttcgagta
    12601 tggccccgca ggggggcgga ttttagcata atatttcgtg cgctgcttcc ctttcgagcc
    12661 gggagatcat gcccccacta aatgcaggca aacatccggg cctacatgac gtatctcttt
    12721 gaatttaaat tggggggcgt cggctaattt ctcaagccct ggcagcgagc ataatccacg
    12781 ggcgtcgctg cctaatagtt taggtgcgat atagacaatc agttcatcga ctaaacccgc
    12841 ctgtagcaat acgccagcga gcgttggccc cgcttccacc cagatgctgt taatctgctg
    12901 tttacccagt tgcatcatca gtacaaccag atccagatga cctttatgct ctggaatcag
    12961 caaggtacgc accgtttccg gccactcacg agaatcttcc tgcgtacgcg cgaaccaggt
    13021 ttcgccgggc tgctgcacaa tgcgatgttc cggcgtcacg cgattttggc tatcaatcac
    13081 aatacgtact ggctggcgga gattttgttg cgggtagagc gcctgagttt gttcatccag
    13141 ttcagaccaa cgcactgtta aggctggatc atccgccagc accgtggcgc tgctggttaa
    13201 aatggcatga ctttgtgcgc gctgtcgttg tacgtcgcgc cgcgcctgag gcgaagtgat
    13261 ccactggctt tcgccgctcg ccatcgccgt gcggccatca agcgatgcgc caagttttaa
    13321 ctgaatataa ggaaagccgg tgcgcatccg cttgagaaag cctttattca attgctcggc
    13381 ttcactcatc atcagcccgt ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc
    13441 acgcccagcg acctgcgggt taggatcttg catcgctgcg accacgcgcg ctacgccagc
    13501 ggcgatgagt gcgtcacagc acggtggcgt acggccatga tggctacagg gttcgagtgt
    13561 cacatacgcc gttgcgcctt tggctttttc acccgccata cgcaacgcgt gtacttcggc
    13621 atgtggttca cccgctcgtt ggtggtaacc ttcaccgaca atttcgccat ctttgacaat
    13681 gacgcacccg acattcgggt tggggtgggt ggtaaaacgt cctcgttgcg ccagctttag
    13741 cgcccgcgcc atgtaatact cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc
    13801 gccaaattct ttgatatctt cgaaactgcg gtagacagag gcaaaacgga tataggcgac
    13861 tttatcgagc tttttcaatt gctccatcac cagattgcca atcatcttgc tcggtacttc
    13921 gcgctcaccg gtggcgcgca gctgcgattt aatatgattg attgccattt caacgtaatc
    13981 ggaactcacc ggacgttttt ccagcgcccg cagcattccg ctacgcaatt tctcttcatt
    14041 aaacggttca cgcacgtcgt tgctttttac aacacgcggc ataaccagct ccgccacttc
    14101 aaaggtggtg aaacgttcat tacacaccag acactgccga cggcggcgta cggatgaacc
    14161 ctcgcccacg agacgagagt caattacctt agtgtccacg gcgaaacaga atgggcaatg
    14221 catacggcgt cctgaccagg tggttaacgg aaatctattt taccctgaac tgacgataca
    14281 acaaaggcgc aacgcttttg agataaagga tcgatgcttt cgctgctttt accctctacc
    14341 attaggctaa ttgcgacgag aaagggacga cctatgaata ctaacgtttt tcgactgctc
    14401 ctgctgggaa gcctgttcag ccttagcgcc tgtgtgcagc aaagtgaagt gcgacagatg
    14461 aaacacagcg tcagcacgct gaaccaggag atgacgcaac tcaatcaaga aacagtcaaa
    14521 atcactcagc aaaacaggct gaatgcaaaa tccagcagtg gggtttacct tctgcctgga
    14581 gcgaaaacac cggcaagact ggaaagccag atcggtactt tacgtatgtc gctggtgaat
    14641 attacgcctg atacagatgg caccaccctt acactacgta ttcagggtga gtcgaatgat
    14701 cccttgcccg cattcagcgg aactgttgaa tatgggcaaa ttcagggaac aatagacaac
    14761 tttcaggaaa tcaatgtgca gaatcaattg attaatgccc ctgccagcgt cctcgccccc
    14821 agcgatgttg atattccgtt acaattaaag gggatttctg tggatcagtt aggctttgta
    14881 cgtatccacg acattcaacc tgttatgcag taaacgtatt ttgggacgat tttgtgcgtc
    14941 ccgcaacatc tttccccgtc attttgttac tctgcttaca tcacttggat tgatagtaaa
    15001 agtttgcaac aagggcgaaa gtcagtacaa tccccgcccg aatgtgtgta aacgtgaacg
    15061 caatcgatta cgtaaatgat agaactgtga aacgaaacat atttttgtga gcaatgattt
    15121 ttataatagg ctcctctgta tacgaaatat ttagaaacgc aatttgcgcc tttttcactc
    15181 ccgcaaggga ttttcaaaca gtggcataca tatgaaaaaa acattactgg cagccggtgc
    15241 ggtactggcg ctctcttcgt cttttactgt caacgcagct gaaaacgaca aaccgcagta
    15301 tctttccgac tggtggcacc agagcgttaa cgttgtcgga agctatcaca cccgtttcgg
    15361 accgcagatc cgcaacgata cctaccttga gtacgaagca ttcgctaaaa aagactggtt
    15421 cgacttctat ggttatgcgg atgcgccggt attcttcggc ggtaactccg atgctaaagg
    15481 tatctggaac cacggttctc cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa
    15541 gctgaccaat actgacctta gcttcggtcc gttcaaagag tggtacttcg cgaacaacta
    15601 catttacgac atgggtcgta ataaagatgg tcgccagagc acctggtaca tgggtctggg
    15661 taccgatatc gacactggcc tgccgatgag cctgtccatg aacgtctatg cgaaatacca
    15721 gtggcagaac tatggcgcag cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa
    15781 atactttgtg ccgattaccg atctgtgggg cggtcagctg agctacatcg gcttcaccaa
    15841 cttcgactgg ggttccgatt taggggatga cagcggtaac gcaatcaacg gtattaagac
    15901 ccgtactaat aactctatcg cttccagcca tattctggct ctgaactacg atcactggca
    15961 ctactctgtc gtagctcgtt actggcacga cggtggtcag tggaacgacg atgcagaact
    16021 gaacttcggc aacggcaact tcaacgttcg ctctaccggc tggggtggtt acctggtagt
    16081 aggttacaac ttctgattat gaaaatgccg ggatttattc ccggcatttc tgattgttaa
    16141 caacatgtgc tttgttcatg ccggatgcgg cgtaaacgct ttgtccggcc cacaaaagca
    16201 cgtaaattca atatattgct gaaataacgt aggcctgata agcgtagcgc atcaggcaat
    16261 tttacgtttt aatcacttct tcttgttcat catcgctttc agatcagcga atgggttgta
    16321 cttcgcttca ccgacatctt tctgcgcatc ttcccctgcg ataacggtcg taccatactg
    16381 atccgcttcg gtatatttcg aatgctcatg atcgtggcaa tagagacaca acaattccca
    16441 gttactgcca tcttccgggt tattggtatg gtcgtgatca atgtggtgaa cggtaagttc
    16501 acgcaggtta gaataaacaa actcgcggga acagcgaccg cagacccacg gatagatttt
    16561 taatgctttt tcgcgatagc cgctttctaa ccgtgcatag ttttttggga tgatagccat
    16621 gaaaagttac ctggatgaaa tgtagggtta cgcgcagttt cccaagatgc ggggattttc
    16681 gcaatctcca tacatcaaaa aaaatcccga tcttctgacc gggattttca acatcaacgg
    16741 gaacttgatt aaggcagaat tgacggctga tccgcccctt ctttttccac tttctgctgc
    16801 aacatgtgtt cgcgcttcat acccagtttc agagccaacg cagatgctac gtagatggaa
    16861 gatgcagtgc cgatggaaac accgataagc atggtcagcg agaagccttc cagtaccgga
    16921 ccaccgaaga gatacagcat caggataacc atcaaggtag taccggatgt gatcaaggta
    16981 cggtgcagcg tctgggtcaa ggacacgtta aagatttcgt aaggcgtacc gcgacggatc
    17041 ttgcggaagt tttcacgaat tcggtccgat accacgatac tgtcgttaag cgagtaaccg
    17101 ataaccgaca tcaacgatgc cacaatggtc aggtcaatct cgatatggaa taacgacaaa
    17161 atacccagcg taatgataac gtcgtgcgcc agcgcaataa ccacccctgc cgccagtcgc
    17221 cactcaaagc ggaaacctac gtacacgagg atagacagca gcgctgccat caacgccatc
    17281 gcaccggttt gcgcaaggtc tgcccccacg ctcggaccga cgaactcaat acgcttcact
    17341 gctgcattct gattggtgga ttcgttaatc accttcagaa cttggctgcc cagcacctga
    17401 ccgccggttt cgccttcagc aggcggcata cggaccatga tgtcgtggct gctaccaaag
    17461 ttttgcagca ttggctcttc aaaaccggct ttttgcaatg catcacgcat tacgtcaatt
    17521 tcagccggtt tttcgagcgt aatttcgata accgtaccac cggtgaaatc cagcccccag
    17581 ttaaagccgc gcacgcccat aataacgata gcagcgatta acagcagacc agagatgccg
    17641 aaagcccagt agtcccagcg cataaagtca tagactttac ggccgtggtt tagttgttca
    17701 acagtatatt cctgtgccac atcgcactcc tcagattgac agcttcttga cgcgcttgcc
    17761 gccatatagc aggtttacga tggcacgcgt accgacaatc gcggtaaaca tcgacgtcgc
    17821 cacaccgata ccggtagtaa tcgcgaaccc tttaattgcc ccggtaccca ctgcgtacag
    17881 gatgatgact ttaatcagcg tggtgatgtt cgcatcgaag atagaactga atgcgccacg
    17941 ataaccttca tcaattgcct gttgaacagt acgtccgttg ctcaactctt ctttaatacg
    18001 ttcgttgatc agtacgttcg catcgaccgc taccgcaagg gttaagacga tacccgcgat
    18061 acctggcata ctcagtgttg cgcctggcaa cagcgacata ataccgacga tcaagatcaa
    18121 gttggcaatc agagcactgg tcgcaatcag accaaacttc ttatagaaga tgatcatgaa
    18181 cagaatagaa accagcagac cggcaaggca agcctccagc ccctgctcaa tgttctgcat
    18241 acccagggtc gggccaattg tacgttcttc aacaatctga atcggcgcga tcaacgcacc
    18301 ggcacgcagc agcagtgaca gctgacgggc ttcgttcggg ttgttgatgc cggtgatacg
    18361 gaagctgtta cccagacgag actggatgtt ggcgatgtta atcacctctt cctgtttcac
    18421 cagaaccgca cgaccatttg catctttctt accgctgtct ttgtactcca caaacagggt
    18481 tgccatcggt ttgccgatgt tgtccttggt gaagttagac ataatgttgc caccagcgct
    18541 atcgagcgag atgttaacct gcggctggtt gtattcgtcc tggctggaag tggagtcggt
    18601 gatatggtca ccagtcagaa ttacgcgttt gtacagcaca accggctgac cttcgcgggt
    18661 ctgtttcact tcagagtcgc ccggtacgcg accggatgcc gcagcggcct gatcaacatt
    18721 ggtgtttacc agacggaatt ccagcgttgc cgtcgcgccc agaatctctt tcgcacgcgc
    18781 agtgtcctga ataccaggca gttcaacaac gatacggtca gcaccctgac gctgaaccac
    18841 cggctccgcc acgccaagtt ggtttacacg gttacgcagg atattaatgt tctgctgcac
    18901 cgcatattca cgcgcttcac tcagacgagc atcgctcatt accgcacgca gctggttgct
    18961 gccctggctg ctaatcacca ggtccggatg gcgcttgctc agatacgcaa tggcttcatc
    19021 acgagcttta gcatcgcgga aagtgatgct caggccgtag ttgttttctt tacgaacagt
    19081 ggtatacggg atgccctttt cgcgcaggtc actgcgtagg ctatcgatat tttgttcctg
    19141 gagtttgcca agcgcggtgt ccatatccac ttccatcagg aagtgaacgc cgccacgcag
    19201 gtcaaggccg agcttcatcg gctcagcatg aatagctgcc agccagcgcg gcgttgccgg
    19261 ggcaaggtta agcgccacga cgtatttgtc acccataacg cccattaatg cttcacgagc
    19321 gcgcaactgg gtgtcagtgg agtcgaagcg cgcaagaata gcgccctctt ccagtgccac
    19381 agacttagca gttatttttt cttcttgtaa cgttttctgg acctggatca gcgtttgctc
    19441 actggcggcg actccgcgcg caccagtgat ctgaacagcc ggatcctcac caaacaggtt
    19501 gggaagcgca tacagcagac cgatgacaat caccacgatc agcatgacgt acttccacaa
    19561 aggataacgg tttaacacgg caattccctt agggaaaaat tttaattaca gcgccttcat
    19621 ggtgcctttc ggcaggacgg cagctacgaa gtcacgttta ataactactt cagtggtgtc
    19681 attcagcgcg atagcaatgt agccgttttc cgctactttg gttacgcgac caaccaggcc
    19741 accgttcgtc agaacttcat cacctttggc aatggagtcc atcagctttt tgtgttcttt
    19801 ggtgcgcttc tgctgtggac gcaggatcat gaaatagaaa atcagaccga ataccaccag
    19861 catcaaaatc aaagacatcg ggctaccttg cgccggtgca cccgttgccg ctaccgcatc
    19921 agaaataaaa aagctcatta aatttccctc attattaata ttaatcaacg ttcaaaggtg
    19981 gtacttctcg cccctgacgc tggtaaaaat cagttacgaa gctctctaat ttaccctctt
    20041 caatagcctt gcgtaaaccc gccatcaaac gctggtagta acgaaggtta tgaatggtgt
    20101 tgagtcgcgc gcctaatatt tcgttgcaac ggtcgagatg atgcaagtaa gcgcgtgaat
    20161 aattgcgaca ggtgtagcaa tcacactcag gatcgagtgg gccagtatcg ctcttatatt
    20221 tcgcattgcg gattttcacc acgccatcgg tcacgaacaa atgaccattt cgggcgttgc
    20281 gggttggcat tacgcagtca aacatatcga taccgcgacg tacgccttca accaggtctt
    20341 ccggtttacc aacgcccatc aggtaacgcg gtttgtctgc cggaatttgc ggacacacat
    20401 gctccagaat acggtgcata tctgctttcg gctcacccac agccagaccg ccgacagcgt
    20461 agccatcaaa gccgatatct accagacctt taacagatat atcacgtaaa tcttcgtaaa
    20521 tactgccctg aattatacca aataacgcat ttttgttacc aagactgtca aaacgctcac
    20581 ggctacgctt cgcccaacgc agagacatct ccatagagcg ttttgcgtaa tcccagtcag
    20641 caggatacgg cgtacactca tcaaagatca tgacgatatc cgaaccaaga tcgtactgaa
    20701 tctccatcga cttttccggg tcgaggaaaa tcgggtcgcc gttgatcggg ttacggaagt
    20761 gaacgccctg ttcggtgatt ttacgaatat caccaaggct gaagacctgg aagccgccgg
    20821 agtcggtaag aatcggtcct ttccactgca taaaatcgtg catatcgccg tgcagtttca
    20881 tgatttcctg gcccgggcgc agccacaggt ggaaagtgtt gccgaggata atttgcgcgc
    20941 cagtggcttc aacttcttcc ggcgtcatcc cttttacggt gccgtaggtg ccaacaggca
    21001 taaaacaagg cgtttccact acgccacgat caaagaccag gcggccacgg cgtgcgcgac
    21061 cgtcggtggt gtccagttca aatttcattt tttctccact acgtcagaaa aacagtccaa
    21121 cgtttaaacc agcgccgcgg aattactccc cgacgcgctc attaattgcc tgcggattgt
    21181 acgtgataaa catcgcatca ccgtaactaa aaaagcgata tttctcttct accgccgctt
    21241 tataggcatt catggtgtgt tgataaccgg caaaggccga aaccagcata atcagcgtcg
    21301 actctggcaa gtggaagttc gtcaccagcg catcgaccac tttgtactgg aagcccggat
    21361 agataaagat ttgggtatcg tcaaagaacg gttcgataag atcgtttttt gccgcctgtg
    21421 ccgcgctttc cagcgaacgt actgaagtgg tgccaaccgc aatcacccgg ttaccgcgcg
    21481 ctttcgccgc cagtaccgcg tctaccacat cctgcggtac ttcagcgtat tccgagtgca
    21541 tgatgtgatc ttcaatggtg tcgacgcgca ccggctggaa ggtgcccgca ccaacgtgca
    21601 acgtcacaaa cgccatctcc acgcctttgg cgcgtaattt ttccagcaaa ggctcgtcaa
    21661 aatgcagacc agcggtcggg gctgcaaccg cgcccggttt ttcgctataa acggtttgat
    21721 aaagttcgcg gtcagcgtct tcgtccggac gatcgatata cggcggcagc ggcatatggc
    21781 cgatgctgtt gaggatatcc agtaccgagc gttcatcatt aaattcgact tcaaacagtg
    21841 cgccgtggcg cgcggtcatt gttgcgttga tactttcgtc atcgcccagc aacagttctg
    21901 cgccaggttt tggcgctttc gaggcgcgaa tatgcgcaag aatgcgtttg tcgtcgagca
    21961 tccgctcaac cagcacttca atcttaccgc cgctggcttt acgcccaaac agacgcgccg
    22021 ggatcacgcg ggtattatta aaaaccagaa gatcgccggg gttgagctta tcgagtaaat
    22081 cggtgaaagt accgtgcgtc agcgcacccg tcggcccgtc cagcgacagt aaacgacagc
    22141 tactgcgttc aggcatggga tagtgggcaa tcagggattc gggcaattca aaggagaaat
    22201 cggtaacgcg catgacactg actcagacta aaataagagt cgggtagtct agtgccgggg
    22261 cgctctccct gcaaccttta cccccctttg gatagaatat aatcgatgaa ttttttagct
    22321 cacctgcatt tagcccatct cgcggaaagc tcgctttctg gcaatttact ggctgatttc
    22381 gtacgcggaa atcctgaaga aagttttccg cccgacgtcg tggctggcat tcatatgcat
    22441 cgacgtattg actgacaatc tgccggaagt ccgcgaagca cgggagtggt ttcgtagtga
    22501 aacgcgccgc gttgcgccta ttacgctgga tgtcatgtgg gatcactttc tttcccgcca
    22561 ctggtcgcag ctgtcgccgg actttccact acaggaattt gtctgttatg cccgcgagca
    22621 agtgatgacg attttgccgg actcaccgcc acgttttatc aatctgaaca attacttgtg
    22681 gtcagagcag tggctggtgc gctatcgcga tatggatttc atccagaacg tgttaaacgg
    22741 catggcaagc cgccgcccac gtctggatgc cctgcgtgac tcctggtacg atttagacgc
    22801 tcattatgac gccctcgaaa cccgcttctg gcagttttat ccgcggatga tggcgcaggc
    22861 gtcacacaag gcgttataaa acgttagttc atccataccg tagccgaaat ggcaggcaaa
    22921 gccagaatcc cgtcagtcag ttgcccatgc ccttctttgc attgccattg cacggcattg
    22981 agaaacggtg acgcgggtag caccacttca ctggcctcgc cacggttgat tgccaccagt
    23041 acacgttgct gattcagcac gcggacaaat accactacgt tatcttccgc atacagcacc
    23101 tgacagccgc cacgacgcag cgcctgactt ttcttacgca gcacaatcat tcgccggtac
    23161 agcgcgaata acgccgtatc ctgcttttcc acctgccagg ggaacggttt acggcaagac
    23221 ggatcgtttt taccatccag tcccacttca tcaccgtaat aaatgcacgg tacaccaggc
    23281 caggtgaaca gccagaccac cgccagcggc aggcgcgcaa tatcccgacc gagcagcgtt
    23341 ttaaatcgcg cagtatcgtg gctatcgagc tgattaaaca tacgtaattg ttgttgatga
    23401 gaaagccctg cgcggtaatt atccatccag gccatacagg tttgggcgtc aatttgctgc
    23461 ggatcataag agatgtcggt attggcaaga aatccccaca acgggaatgt gaagccacga
    23521 tagttcatgg cagcatcttc cacatcggcc tgtaaccatt gccgtgcatc gccaaaatgt
    23581 tcgccgacaa tatacgcttc cgactgggtt tctttcgccg cttcggtgat cccggcaacg
    23641 tgctgcatat tattgcgcgc cccacccgcc tcccccagca tatgcaccac atccagccgc
    23701 cagccgtcca tattccacgg cgctttcagc cagtggcgga caatactgtc ttccccgcga
    23761 taaatttcat tcaccagact ttccgactga taatccagct tcggcaagct ggcatagcca
    23821 agccagtcga gcgccgtgcc atcatcacta aacaagtacc agtcgcgcca gggcgattca
    23881 gagttgtgac aagctccccc cgtgccaaga ttgtgcctgt ca
//
LOCUS       DADLTF010000055        23693 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.055, whole genome shotgun sequence.
ACCESSION   DADLTF010000055 DADLTF010000000
VERSION     DADLTF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.055"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="JN749_002030"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="JN749_002030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAY7368453.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="JN749_002031"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="JN749_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAY7368454.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="JN749_002032"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="JN749_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAY7368455.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="JN749_002033"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="JN749_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAY7368456.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3880..3924
                     /locus_tag="JN749_002034"
     CDS             3880..3924
                     /locus_tag="JN749_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HAY7368457.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="JN749_002035"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="JN749_002035"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAY7368458.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="JN749_002036"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="JN749_002036"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAY7368459.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRIAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="JN749_002037"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="JN749_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HAY7368460.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="JN749_002038"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="JN749_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HAY7368461.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="JN749_002039"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="JN749_002039"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HAY7368462.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="JN749_002040"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="JN749_002040"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HAY7368463.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="JN749_002041"
     CDS             10418..10882
                     /locus_tag="JN749_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7368464.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="JN749_002042"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="JN749_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13298
                     /locus_tag="JN749_002043"
     CDS             11895..13298
                     /locus_tag="JN749_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HAY7368465.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPD"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JN749_002044"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="JN749_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HAY7368466.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JN749_002045"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="JN749_002045"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HAY7368467.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JN749_002046"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="JN749_002046"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HAY7368468.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="JN749_002047"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="JN749_002047"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HAY7368469.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JN749_002048"
                     /pseudo
     CDS             complement(18461..20161)
                     /gene="fadK"
                     /locus_tag="JN749_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707412.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="JN749_002049"
     CDS             complement(20164..20457)
                     /locus_tag="JN749_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HAY7368470.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="JN749_002050"
     CDS             complement(20454..21743)
                     /locus_tag="JN749_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAY7368471.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFIYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21799..22737)
                     /locus_tag="JN749_002051"
     CDS             complement(21799..22737)
                     /locus_tag="JN749_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HAY7368472.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIISLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTQGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22757..23521)
                     /locus_tag="JN749_002052"
     CDS             complement(22757..23521)
                     /locus_tag="JN749_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HAY7368473.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5846 a         5780 c         6243 g         5824 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agtatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatagcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgctgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcaacaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgagg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaggcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaaccaata cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagataa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt agcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 cccctgcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggaaatga tcgccagcgg
    22321 gctgttgatc ttttcctgag cgaacgctaa cccgccatac atccggtgtt cggcgcaaat
    22381 gtgaccatcg acaatatcca ccgccgtggc atcgttcacc agcgccgcat tcagttgcac
    22441 acttaaccgt gctgccagag ctttaccacg tttcgtcgcg gccaacagca acatagcggg
    22501 gtgtttatct ttcagcaggg cagcaatgct ttcggcgtaa ttttcagtgc gttgcagcgc
    22561 gtcgttttgc tcaagaacat aaatacattt tggaccataa ggcataactg cctgcgcctg
    22621 gtcggtattt tgtacaatgg catacacctg ttggccccat tgctgagcgc cgccaaacag
    22681 ttcagcataa cgttcaggat tatcgctaaa gacccagacg ctgtttaatt gactcatcgt
    22741 ttcttctccg taggcttcag ttcagggctt tcttcagatg ttccgccagc tcggcaatgg
    22801 cctccggcga atcgttatcg agaatgatgt gcttacgttc tgtttgcggc ggtacgcgaa
    22861 tgccgaccag ttcagcaagt ggcgcgctct ggctccagtc aatatcactt gcctgccact
    22921 gatttaccgg ttttttaccc gcgccgagaa tggctttcat cgaaggaatg cgtggcacgt
    22981 taatatcgga ggtgacgcag agcacggctg gaacagagag ttcaataact tcaacatcat
    23041 cttcaagcgt gcgttcaatc accagtgtat tgccctgacg ctgaatagca ctcactgcat
    23101 taatcaccgg aagttgcaga atttctccga ccagcaagcc aacctgctgg gcataaaggt
    23161 cgccggaacc ttcaccaaag atcagtaaat cgaagccgat cttttcaatt gctgccgcca
    23221 gcgcttttgc ggtatcgaga ggcagtgcat gttcaagttg cgcatcctgc accaaataca
    23281 ggctgtgcgg cccgcgggat agcacgtctt tgcgcacttt cgagttctgc aacaatgagc
    23341 cgccaacggt cagcgcggct atctcatcgt catctgttgc gagctggctt gcagcttcaa
    23401 tggcattgag atcgaactgg ctgattttgg cgtcggcatt gtcgaaattc agggtgtatt
    23461 ctggagtgac aacaatgtcc tgttcttcag gcaccagctt aaagcaggtt attattttca
    23521 tgatttctcc tgtgagtcgc atacggaacc atctccgatg gaaggtaaaa atggcctgaa
    23581 aatagtgtgc gatagcggtg cggggggttc gacggagcaa gctgttttcc ttgccaacaa
    23641 tcttgcaaaa aaaatgcgat tgctaaaaca aaagtgcaat ttgcggcaat ata
//
LOCUS       DADLTF010000056        23163 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.056, whole genome shotgun sequence.
ACCESSION   DADLTF010000056 DADLTF010000000
VERSION     DADLTF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23163
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.056"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(269..886)
                     /gene="tdk"
                     /locus_tag="JN749_002053"
     CDS             complement(269..886)
                     /gene="tdk"
                     /locus_tag="JN749_002053"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAY7368474.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQT
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1491..1904
                     /gene="hns"
                     /locus_tag="JN749_002054"
     CDS             1491..1904
                     /gene="hns"
                     /locus_tag="JN749_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HAY7368475.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JN749_002055"
     CDS             complement(2048..2956)
                     /gene="galU"
                     /locus_tag="JN749_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAY7368476.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JN749_002056"
     CDS             complement(3158..4171)
                     /gene="rssB"
                     /locus_tag="JN749_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HAY7368477.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JN749_002057"
     CDS             complement(4263..5168)
                     /gene="rssA"
                     /locus_tag="JN749_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HAY7368478.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5281..5739
                     /locus_tag="JN749_002058"
     CDS             5281..5739
                     /locus_tag="JN749_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HAY7368479.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5789..6631
                     /gene="purU"
                     /locus_tag="JN749_002059"
     CDS             5789..6631
                     /gene="purU"
                     /locus_tag="JN749_002059"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HAY7368480.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6791..6875
                     /locus_tag="JN749_002060"
     tRNA            6791..6875
                     /locus_tag="JN749_002060"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6825..6827,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="JN749_002061"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="JN749_002061"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JN749_002062"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="JN749_002062"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HAY7368481.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JN749_002063"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="JN749_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HAY7368482.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JN749_002064"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="JN749_002064"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7368483.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="JN749_002065"
     CDS             complement(10156..13899)
                     /locus_tag="JN749_002065"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HAY7368484.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JN749_002066"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="JN749_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HAY7368485.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="JN749_002067"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="JN749_002067"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HAY7368486.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="JN749_002068"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="JN749_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HAY7368487.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVIFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JN749_002069"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="JN749_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HAY7368488.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="JN749_002070"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="JN749_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HAY7368489.1"
                     /translation="MKKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /locus_tag="JN749_002071"
     CDS             complement(20438..21133)
                     /locus_tag="JN749_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7368490.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JN749_002072"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="JN749_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HAY7368491.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="JN749_002073"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="JN749_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HAY7368492.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
BASE COUNT         5801 a         6046 c         5572 g         5744 t
ORIGIN      
        1 ttctggggcc gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc
       61 ttcatcatat ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc
      121 acaagtcaac gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgcc
      181 gagaagggta tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct
      241 gctcaccctg tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg
      301 ttaatgagcc gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt
      361 cattaccacc aattaccacc tgctcacctt cgttataagg tctgcctgtt tgatcaagac
      421 gcagcaccat gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt
      481 cggaccatgc cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta
      541 aaccataaca aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt
      601 gttgtctggt taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat
      661 gttccgcacg aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg
      721 acaaacctat acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat
      781 atacgacagt gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag
      841 acttacccgc attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct
      901 caatgatatg aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac
      961 agtatgcaca gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga
     1021 gctatcatta caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca
     1081 caatctttaa cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat
     1141 actaagtcca tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt
     1201 ctcgtaaaca caactaatac agaagactga aaggtcgtca gcctacgata atctccccat
     1261 aaaatgtgac atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat
     1321 agggctatat gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca
     1381 aaataaagaa caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc
     1441 tctattatta cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag
     1501 cacttaaaat tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg
     1561 aaacgctgga agaaatgctg gaaaaattag aagttgttgt taatgaacgt cgcgaagaag
     1621 aaagcgcggc tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa
     1681 tgctgatcgc tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat
     1741 ctggcaccaa agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg
     1801 gcgaaactaa aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg
     1861 atgagcaagg taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca
     1921 cttgcttaaa atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata
     1981 agaataagct ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat
     2041 acggatgtta cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa
     2101 gggtgttatg acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc
     2161 agtcatggct cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat
     2221 caattgcgtc ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg
     2281 gccaaatatc cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat
     2341 ccgctttcgg tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa
     2401 cgcctttgca atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct
     2461 ggctatgacc cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat
     2521 cggattcata ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca
     2581 ctaccgggtg agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt
     2641 gcataatagt cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta
     2701 cacgtttttc cagcattgcc tccagttcaa aactggtatc aaagtggttt tcaatagagt
     2761 ttttagatga gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca
     2821 cgacgtattg aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg
     2881 tcgccggcaa catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg
     2941 tattaatggc agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt
     3001 ctgtatcgca tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag
     3061 atagcataat taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt
     3121 agcaggtaat gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc
     3181 gcagtcgacc accggttccc catatttggc attgccaagc atcgcatcgc tggctcaatt
     3241 gattcaaata agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt
     3301 gttcgccggt atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt
     3361 cgcgatgata atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta
     3421 aatggtttac ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa
     3481 gctgttcctg taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat
     3541 tatgtccagc tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg
     3601 caatatcaag caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc
     3661 aatgggaaat cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg
     3721 cagggttatc aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg
     3781 aattaaacat gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt
     3841 taactggttt cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca
     3901 tattttcagt ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct
     3961 ccagcagttt aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag
     4021 tgaaacctcc cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc
     4081 ccaatgagga aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga
     4141 gaatctgttt tccgaccaat ggctgcgtca tgttctctcc cctgaccggc tttactcaat
     4201 agtggcatgc tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct
     4261 ggtcaaatgt tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc
     4321 tgtccggccg caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa
     4381 acaggttgaa tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc
     4441 tccaacacct ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga
     4501 cgtgtcgtta tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca
     4561 ttctcgctat tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca
     4621 tcgtgctgca ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata
     4681 ggaattgggt taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc
     4741 atgagtcctg gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac
     4801 cataattcac gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt
     4861 tcgatctctg tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt
     4921 aacccaccgc gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag
     4981 gttacccaat cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa
     5041 ccaattgaac atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata
     5101 acgccaatat gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc
     5161 tttctcatta ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc
     5221 ggttaacata gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattg
     5281 gtgtctcagc tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat
     5341 gtgtctggtg aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct
     5401 tttgtgatgc aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg
     5461 gcgttacgtg ccgaattgat gaccggattt gcgcatactg aatggttggg attgaccgta
     5521 tttgaacatt gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt
     5581 accgaaggtg gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt
     5641 caatggtact atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt
     5701 ggttcaggta aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc
     5761 aaacactctc aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac
     5821 tatttgtccg gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga
     5881 gttaaatatc gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg
     5941 cacggaactg gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt
     6001 gccagaaggc tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt
     6061 cactaaagaa gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga
     6121 tgtcgaaatc gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt
     6181 tgatattccg tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa
     6241 gatggcggac gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg
     6301 ggtattaacg ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc
     6361 attcctgcct gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa
     6421 gattattggc gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat
     6481 gcaggacgtt attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg
     6541 tgacgtcgag aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt
     6601 tgtttacggt aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta
     6661 atccgttacg gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc
     6721 ggcgcgtcat ttgatatgat gcgccccgct ccccgataag ggagcaggct agtaaaaagc
     6781 attaccccgt ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6841 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct
     6901 caaacgaatc tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 agccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagcgcacg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact
     9181 gctgtgcagc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacggac
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt
     9901 tacccagcag catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg
    11221 ataatcgcgc tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgctttta gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag
    14341 gcgatataga aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa accgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt
    15661 tcggaggcag atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgga gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatatatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg
    16381 agaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg
    16621 cgcaaacatc agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actggtggat accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tgatattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggctatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgca tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt
    18961 tgattttcgc cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc aaagttaaac
    19081 aaatcaacac gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg attttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgctacaa gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgaaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca
    20461 caccatccgg ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcatcagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccagaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtttt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc
    22561 ctttactggc ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aacaatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 gcagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggagagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggt
//
LOCUS       DADLTF010000057        22969 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.057, whole genome shotgun sequence.
ACCESSION   DADLTF010000057 DADLTF010000000
VERSION     DADLTF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.057"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(71..4688)
                     /locus_tag="JN749_002074"
                     /pseudo
     CDS             complement(71..4688)
                     /locus_tag="JN749_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(4781..5428)
                     /gene="rnt"
                     /locus_tag="JN749_002075"
     CDS             complement(4781..5428)
                     /gene="rnt"
                     /locus_tag="JN749_002075"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HAY7368493.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5531..5938)
                     /gene="gloA"
                     /locus_tag="JN749_002076"
     CDS             complement(5531..5938)
                     /gene="gloA"
                     /locus_tag="JN749_002076"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAY7368494.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6019..7116)
                     /gene="nemA"
                     /locus_tag="JN749_002077"
     CDS             complement(6019..7116)
                     /gene="nemA"
                     /locus_tag="JN749_002077"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HAY7368495.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7153..7752)
                     /locus_tag="JN749_002078"
     CDS             complement(7153..7752)
                     /locus_tag="JN749_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7368496.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7855..8094
                     /locus_tag="JN749_002079"
     CDS             7855..8094
                     /locus_tag="JN749_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HAY7368497.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8143..9039
                     /locus_tag="JN749_002080"
     CDS             8143..9039
                     /locus_tag="JN749_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAY7368498.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(9294..9803)
                     /locus_tag="JN749_002081"
                     /pseudo
     CDS             complement(9294..9803)
                     /locus_tag="JN749_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9796..10587)
                     /locus_tag="JN749_002082"
                     /pseudo
     CDS             complement(9796..10587)
                     /locus_tag="JN749_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            complement(10590..10826)
                     /locus_tag="JN749_002083"
     CDS             complement(10590..10826)
                     /locus_tag="JN749_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HAY7368499.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            11027..11461
                     /gene="slyA"
                     /locus_tag="JN749_002084"
     CDS             11027..11461
                     /gene="slyA"
                     /locus_tag="JN749_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HAY7368500.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(11508..11975)
                     /gene="slyB"
                     /locus_tag="JN749_002085"
     CDS             complement(11508..11975)
                     /gene="slyB"
                     /locus_tag="JN749_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HAY7368501.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            12249..13358
                     /gene="anmK"
                     /locus_tag="JN749_002086"
     CDS             12249..13358
                     /gene="anmK"
                     /locus_tag="JN749_002086"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HAY7368502.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            13456..13785
                     /gene="mliC"
                     /locus_tag="JN749_002087"
     CDS             13456..13785
                     /gene="mliC"
                     /locus_tag="JN749_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HAY7368503.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGLSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            13844..14500
                     /gene="pdxH"
                     /locus_tag="JN749_002088"
     CDS             13844..14500
                     /gene="pdxH"
                     /locus_tag="JN749_002088"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HAY7368504.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            14629..15903
                     /gene="tyrS"
                     /locus_tag="JN749_002089"
     CDS             14629..15903
                     /gene="tyrS"
                     /locus_tag="JN749_002089"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAY7368505.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            15965..16831
                     /gene="pdxY"
                     /locus_tag="JN749_002090"
     CDS             15965..16831
                     /gene="pdxY"
                     /locus_tag="JN749_002090"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HAY7368506.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVK
                     AANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVN
                     NVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMR
                     QPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRI
                     AKPEHYFSATKL"
     gene            complement(16875..17480)
                     /gene="gstA"
                     /locus_tag="JN749_002091"
     CDS             complement(16875..17480)
                     /gene="gstA"
                     /locus_tag="JN749_002091"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HAY7368507.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(17586..19088)
                     /gene="dtpA"
                     /locus_tag="JN749_002092"
     CDS             complement(17586..19088)
                     /gene="dtpA"
                     /locus_tag="JN749_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HAY7368508.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(19699..20334)
                     /gene="nth"
                     /locus_tag="JN749_002093"
     CDS             complement(19699..20334)
                     /gene="nth"
                     /locus_tag="JN749_002093"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAY7368509.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(20334..21029)
                     /locus_tag="JN749_002094"
     CDS             complement(20334..21029)
                     /locus_tag="JN749_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HAY7368510.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(21033..21653)
                     /gene="rsxG"
                     /locus_tag="JN749_002095"
     CDS             complement(21033..21653)
                     /gene="rsxG"
                     /locus_tag="JN749_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HAY7368511.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(21657..22715)
                     /gene="rsxD"
                     /locus_tag="JN749_002096"
     CDS             complement(21657..22715)
                     /gene="rsxD"
                     /locus_tag="JN749_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HAY7368512.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(22716..>22969)
                     /locus_tag="JN749_002097"
     CDS             complement(22716..>22969)
                     /locus_tag="JN749_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="part of membrane-bound complex thought to be
                     involved in electron transport to nitrogen; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7368513.1"
                     /translation="AIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQ
                     QQANAEPEEQVDPRKAAVAAAIARVQAKKAAQQKVVNED"
BASE COUNT         5647 a         5942 c         5975 g         5405 t
ORIGIN      
        1 accgaaatga ttcaggcgac agcttgaacg tagcagggaa ccacgtcttt cagggcgtgg
       61 aggatgtcaa ctatccccaa tccagccctt gtggcgaact tgaaaatccc gcctcacgca
      121 gcagcgtaaa catcggcgtt tgccggaccg gaagatcatt cacttctgtt agcgtaaagc
      181 gcagccttgg ttcgcgacgc agtgcggtgg tcagcgcctg gaaaatctct ggggcgagta
      241 attcctcctt ttcctgccag accagcattt ttttgccgcc ttgcggcaga tagagtaaca
      301 atttgccgcc agaaacgacc accagcgcac ccgcccgacg cgttggtacc agcgctgccg
      361 gatgtgcagg ccagggcaga agatttcccc acacattagc cggatcgttg gcggagagcg
      421 ccactggtgt atagtggcgt gtttgcgtgg cttgtgtcgc cagatcgcgc aatcggtcaa
      481 tagtcagacg ttcagcgaat tgcgcgccac ccagaccttc tacaaaacga cctcgcataa
      541 ttcgcccgga gtcttccata cttcgacaaa gtgtttgcat cgatggaaac ccgccaggga
      601 tattttcggc tatcaccgcc tgacgactga tgatgccgta gcggtcgagc atattttccg
      661 ccagcgccag catcctttcg gtatcgttta gtggctccac ctgcaataac gaccagcgtc
      721 cagccagatt tggtgtgttg taagataccc gcggcgagac gggttgtgca tagacaggac
      781 gcccacgacg agcccggtga ctgcggcgag ttgaggtgcg tgcgttggaa ctgctgcggg
      841 tgagggcgcg taacggtgcc caaatatcgc tggtaatgac gccttgccag accagcgccc
      901 ataacgcctc ttgcagtgcc gaggaatcaa ccgattcgcc gattttatcg cgtatccgtt
      961 ggctgatttg ttgtgcaaac caggctcctc cgtcagccag aacagcgatt atcgcttgct
     1021 gcagcgccga acgattcgcc tgatccgctt cggcgggagt gaacgattct gcggcatatt
     1081 tctgtagatg cagtgccacc aggccgtcat cttcacccag cttttttttg tcccgaccag
     1141 ataaccgctc cggttgccag taattcatcg agcatttctg gcgaatagtc gcgtacgcgg
     1201 gcaggcagga tctggctttc ccagagtgag gcgggtaaac cgactccggc aagctgttcg
     1261 atcacccgca tcacgccatc tacgccctca taaacgcctg gcgatgttga ggcaaagagc
     1321 gccgggctac catcggtggc gggtaatacg ccctgacgtt ccagcagcaa tcgcgcatag
     1381 gtcgtggctg caacgggacg cgtcgcttct ctggcggctt gcagcgagcg caaacgcaga
     1441 cgacgaaata cttcatcgct cacccagatg tcttgttgca gattcatcac cagaccctgt
     1501 tcacgcagtt gctgaagctg ctcttcgaca atggcaatac cgagactaaa ctcatgagcc
     1561 agttgttcag ccgtgaccag agcatgagcc cggagatagc gcagaaagag gtcgcgaagc
     1621 gggtaactga ctctatgtaa ataaatctct ggcaatgact ctggtagtcg tacgccgagg
     1681 gcatcacgca gcctggcggc atcatccata caggcaagac gctcctgtcc gctaatcatc
     1741 gcagggaaga ttcgttttac tgcaagaaga ttttccagat acgacgcaac ctcttcactg
     1801 ctgcctgtat gccgttgcgc caggtcttca acggtcattg gccccagttc gcgcagcagg
     1861 tcgaacaatc cttcttcacc tttcgctctt ctgccaggag ccagtcgttg caactcttct
     1921 tccacctggc gaatgacctg cgggtcgagt aattcccccg gatcgacctg tccgagtaga
     1981 ttgcgcagta actcgctgtc cagcgacagt acggatgccc ggcgctctgc cagcggggcg
     2041 tcgctctggt acataaattc cgcgacatag ccgaacaata aacttgtggc gaaaggcgag
     2101 ggcgtagtgg tcgttatatc ggatatttga atttcgccac cgttcaggcg acgcatcaaa
     2161 cgttccagtg cgggaagatc ataaacatct tgcagacatt cgcgtagggt ttcgagaatg
     2221 accggaaaat ccggatatcc ctgagcgatt tccagcaact gactggcgcg cagccgttgt
     2281 tgccaaagcg gggtgcgatg gcccggcgtg cggcccggca ttaataatgc ccgcgcggcg
     2341 cattcgcgaa aacgggcggc gaaaagtgcc gagctgccta ccgcctcgcg gacaatttgc
     2401 agcaactttt ctggttcaaa caaaaaaatc gcggcgtcgg gcaatttacc atcagtgtca
     2461 ggaatgcgcg caacaatgcc gtcatcactg gcgaccacag acgcgtcagc gccccatagg
     2521 gcatgtattc gcccggcaat cgccagcgcc cagggttcat gcacccgtct tccataggga
     2581 gagtgcaaaa taatacgcca gtcgccaatt tcatcacggc accgttcgag gaccagatgg
     2641 cgactccccg gaacgatgcc cgtcgcgttg cgttgctcgt caatcaaccc ctgaatattg
     2701 gcgttcgtat tttcttctgc cagccacggg ggaatagtcc cggaaaagaa cgcgccatcc
     2761 gccagtaaat gaagaaaatc gccgatcatc tcgcctaatt cagccggacg tccgttacct
     2821 tcaccacgcc agaaggggag ccgggcagaa cgacccggag caggagtcac aatcacctga
     2881 tcgcgggtga tttgctggat ccgccatgag gtagcgccga gagtgataat gtcgttcacc
     2941 cgcgactcat ataccatctc ctcatccagt tcacccaccc gccgcgaacc ggccttttct
     3001 tcaccttcgg gtaataacac gctatacatg ccacgatccg gaatggtgcc gccgctggta
     3061 accgccaaca attgagcgcc aggtcgggcg gtcaatatcc cggtctccct gttccagacc
     3121 agtttggggc gaaaagcaga aaaatcgcca gagggatagc gcccggaaag catatccagc
     3181 gtggcgtcaa aaacacgtct tggcagatct ttccacggtg cggcacggcg tacgcgggag
     3241 taccattcgt ctacctgtaa tgcatccatc gccgcggcgg caacggtttg ctgcgcaagg
     3301 acgtcgagag gattatgcgg tggtgtcagg ttttccagcc tgcctgcgaa catacactct
     3361 acaatgactg cggaatcgac taaatcacgc cgggtacggg ggaaaaacag ccctttagat
     3421 acaccgccaa cctgatgtcc ggcgcgacca atgcgttgta acccactggc aacagaaagc
     3481 ggcgttgcca cctgaatcac cagatccacc gcgcccatat caatccccag ttcaagactg
     3541 gaggttgcga ccacgcagcg taattcaccc gatttcagcg cctgttcggt gattgctcgt
     3601 tgttctttag agacggagcc gtggtgtgag cgggcaataa aaacgtcgct actttgtaca
     3661 cggttagagg ttgcgccgga ggtcgactcg aaatgggccg catcaacggc gatagacggg
     3721 gaacgctgta agcgtgcggc gtaaagctca tttaatcgtg ccgtcagttt ttccgccagc
     3781 ccgcgtgaat tagtaaagac aatggtcgag cgatggcgca acacttcatc aaggataccc
     3841 gtttcaatat atggccagat ggagccttcc cggccggcat ggctgtcttc gccggtgccg
     3901 ctggcgaccg atgagacatc atccatattg gcgaccggta cgacaattcg tatctgcgga
     3961 tggcgcattg cgggcgggtt gactaccgta accgggcgat cgccaccaag aaatgctgcc
     4021 acatcgctgg ctgagcgcac agtggcagaa aggccaattc gctgtgctga ggtgtggagc
     4081 agcgcatcga gccgctccag acttaacgcc agatgcgcac cacgtttact gcccgctacc
     4141 gcgtggactt catcaataat taccgtttcg acgccgtgta gcgtttcgcg cgcgcgggag
     4201 gtcagcatca gatagagtga ttcgggtgtg gtgatcagaa tatccggcgg attacgggtg
     4261 agtttgctgc gttcctgtgc aggcgtatcg ccagtacgga tccctacgcg aagattgact
     4321 tccgtttcgc cgcgccgccg ccgttcatcg gcaataccct tcaacaggat ctgcaagttg
     4381 cgctgaacgt cggtgcccag ggcttttatc ggtgaaatat agaggatgcg tgaggtttta
     4441 cgcttatgcg cctcgcgggt atcttcgccg ccttcgcgga agagccgatc gagggcgtag
     4501 agaaatgctg ccagcgtttt cccggagcca gtcggtgcaa tcaccagcgc atgttcgcct
     4561 cgcgccgcca catgccaggt ttgcgactgg acagcggtcg gctgtttaaa ggcgcgaaga
     4621 aaccagtcgc gggtcgccgg tgaaaacacg tccggcagga gcgatgaagg gtctggatta
     4681 tctgccatta acgctattcc ccgtgaatat catgcttgat gcgtggaata gcctcactat
     4741 agatgcctga atcattgcat gtcatcaggc atcgactcga ttacacctct tcggcggcag
     4801 gtagcggcca gcctcccaga cgtttccagc ggttgacgat ttcacaaaac agcacagcag
     4861 tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct gtcgaagtcc atgccagcgg
     4921 tctggcaagc ctttgacaat acggtttgtc cgagtgccag cccggccagt gcagcagtgt
     4981 caaaagtggc gaaagggtgg aacgggttac gtttcagcga ggcgcgttct gcggcggcca
     5041 tcataaagct gtgatcaaaa ttggcgttgt gcgccaccat aatggcgcgg ttacagccgc
     5101 tcgctttaat acctttacgt acaactttaa aaatttcgtg cagcgcctcg tattcgctga
     5161 ccgcgccgcg atcgggatcg ttcgggtcaa tgccgttgaa ggcgagggct tctggttgca
     5221 aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt ggtgtccggc atcagccagc
     5281 cttgttcatc cattttcagg gtgatggcgg caatctcaag cagcgcatcg gttttggcgt
     5341 taaatccggc tgtttcaaca tcgatcacaa caggataaaa accacgaaaa cggtcgcaca
     5401 gaccggtaag ttgagcgtta tcggacatct gattctctta atacggataa attgcagcgc
     5461 gcattatgac aaatattact tgtcgatgca gtaaagatgc gggcgcgatg agttcacgcc
     5521 cggcaggaga ttagttgccc agaccgcgac cggcgtcttt ctcttcgatt aactcaattt
     5581 tgtaaccgtc cggatcttcc acaaacgcga taaccgtagt accgcctttt accggacccg
     5641 cctcacgggt cacgttaccc ccgttttgac ggattttttc gcacgcttca gcggcgttat
     5701 ctacgctaag cgcgatgtga ccataagcag tgccgagttc gtatttatcc acgccccagt
     5761 tgtaggtcag ttcaatcacc gcttcttcgg tttccgggcc gtagccaaca aacgccagtg
     5821 agtatttgta ttccgggttt tcgctggtac gcagcagttt catgcccagc actttggtat
     5881 aaaaatcgat ggagcgttgc aaatcgccaa cgcgcagcat ggtatgaaga agacgcatct
     5941 ttttatcctc aaaatggcta acagattcaa aatgttgttt tagtatagcg gcggctttac
     6001 gccgctcgca atgttggatt acaacgtcgg gtaatcggta tagccttccg cgccgccacc
     6061 gtagaaactt tcggcacgct gtgggttaag ctcagctttg agctgcaagc gggcgaccag
     6121 atccgggttc gcaatccagt cacgaccaaa tgccaccgca tcaattaacc ctttgccgat
     6181 cagcgtttca gctttttcta ctgtgtatgc acctgcgccg ataatcggac cgtggaaacg
     6241 ggcgcgtact ttttcgcgga acgcatcagt atacggttca ccccccgccc aatctggttc
     6301 tgacatatgc agataagcaa tgccgcgttt acccagttgt tcaatcagat acagtgcatc
     6361 ggcttcttca ttcgggccgt tatctgtgtt ctggaaagta ccgattggcg aaacgcgaat
     6421 gccaatgcga tcggcacccc attcttcaat cccggcatcg accacttcca gtaccaaacg
     6481 tgcgcgattt tccacgctgc cgccgtactg atcggtacga tggtttgaag aaggggaaag
     6541 gaactgatgc agcaaataac cgtgagcaga gtggagctct accagatcaa aaccggcttc
     6601 acgcgcgtta gcaatggcct gacggaaatc attgacgata cccggaatct cttccagttc
     6661 aagcgcacgc ggcatggatg tttcaacgcg gatcgcccga ccattttcat cgcgcagaga
     6721 agtacgtgtt cccgcgctaa gtgctgaagg cgctaccggt gcctgaccgc caggttgcag
     6781 gctggcgtga gaaatgcgtc cggtgtgcca cagctgcaag gccatatgac cattttcagc
     6841 atgaacgcca gcggtgattt ttttccatgc ggcaatttgc tccggactat ggatgccagg
     6901 cgcacctgca tatccttttg cctgggcaga aatttgcgtg gcttcactaa taatcaaacc
     6961 ggcactggca cgttggcgat agtattccgc catcaacggg gtaggaatgt cacccggttc
     7021 aatactgcgc agacgcgtca gcggtgccat aaaaatacgg tttgccgccg tgatcgcgcc
     7081 cactttcagt ggggaataca gtttttcaga tgacataaag gctcccggtc gtctggtgat
     7141 aaaaaatgcc tgctaaacgg caggcgtcgc aataatgttt tttacatggg ccagcgcgtt
     7201 ttccagtggc tcgaaactgc gcgaaatttt ggcctgcaga ttcgcgccaa gccatagtgc
     7261 gtaaagcact tgtgcctgtt gtagcggttc gccacaaaag gttaaacaat ggttctcacg
     7321 gccattttcc agagcctgcg agagcagggc gatcacgccg cgagcacctt tatccatcgc
     7381 gctgcgcata tcttctgaca gatcgcacac ttcggcagag agttttactg tcaggcaacc
     7441 actgatggtt ccatgttggc aaaactggtt cagtgtttgc tggtaataag ccagtatgcg
     7501 gtcgcggtag ttaccttcgc cggattgcag caactcagcc agtcgctggt gatatgcggc
     7561 gtaatgacgc tcaagcatgg caacgccaaa cgcttcttta gagcgaaagt agtgatagaa
     7621 ggaccctttc ggcacttcag cggtttttag taattcgctt agccccatcc cggtgaatcc
     7681 acgttgcagg caaagttgct cgcccgtcgc caagagatgt tcgcgagtat catgttcggt
     7741 gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc tagtgcaagc aaagttgcta
     7801 ctttggacat taacagcttc aataatggta ataatcttat ctgaggaggg cgaggtggcg
     7861 gagcaattag agttctttcc cgtccagagc ccgtgccggg gaatttgcca gtctgacgaa
     7921 cgcgggtttt gtcgcggctg ttttcgtagt cgggatgagc gttttaactg gaataaaatg
     7981 agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc agcgattaat gcgcaaatta
     8041 cgcgcaaata agccagcgtc atcagacgaa ccggagcaac catcactctt ttgaacctaa
     8101 tcttgcgtat actcctctca tatctgataa gaggaagcga ttatggttca gcgtattact
     8161 attgcgccgg aaggcccgga gttttcccgt tttgtgatgg gctactggcg attgatggac
     8221 tggaatatgt ccgcccgcca gctggtcagt tttattgaag agcatctgga tctcggcgtg
     8281 accaccgtgg accatgctga tatttatggt ggctatcagt gcgaagcggc gtttggcgag
     8341 gcactgaaac tggcacctca cctgcgtgaa cggatggaaa tcgtcagtaa atgcggtatc
     8401 gcgacgaccg cgcgtgaaga aaacgtcatt ggtcattaca tcactgaccg caatcacatc
     8461 attaagagcg ccgaacagtc gctaattaat ctcgcgaccg atcatctgga tctgctgtta
     8521 atccaccgac cagacccgtt aatggatgcc gatgaagtgg cggacgcgtt caaacatctg
     8581 catcagagcg gcaaagtgcg tcattttggc gtatcgaact ttacgcctgc gcaatttgcc
     8641 ctgttgcaat cacgtctgcc gtttaccctt gccactaatc aggtggaaat atccccggtg
     8701 catcagccgt tactgctgga tggcacgctc gaccaactac aacaactgcg tgttcgtccg
     8761 atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg atgattattt ccagccgctg
     8821 cgtgatgaac tggctgtggt ggcggaggag ttaaacgcgg gctcgattga acaggtggtt
     8881 tacgcctggg tattacgttt accgtcgcag ccgctgccaa ttatcggctc aggtaaaatt
     8941 gagcgcgtac gggcagctgt cgaagcagaa acactgaaaa tgacccgtca acaatggttt
     9001 cgtatccgta aagctgcact ggggtacgac gtaccgtaag cacttttagg aatagccgcc
     9061 gttcaaaaat gtgtcactgg tttacactta ttcaggaatg cacaatgaac ggaggtccta
     9121 tgaaacgttt tagtctggcc attctggcgc tggttgttgc gaccggcgca caagctgcca
     9181 gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt tgggcagtca attggtagcg
     9241 tcaccattac tgaaactgat aaaggtctgg agttttcacc cgatctgaaa gcattacccc
     9301 ccggtgaaca tggcttccat attcatgcca aaggaagctg ccagccagcc accaaagatg
     9361 gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga tccacaaaat accggtaaaa
     9421 ttaaccatcg gaaaggcgat gatggcagcc gtgtagcccg ccagttgaaa tgcatacgcg
     9481 acgttattcg tgaagtgcgc acaggcctag gtacaaaagc caagccacgc cgacatgctc
     9541 aatagaaaaa accacggctc attgagcgta tgtccggcaa gaagcagtgc cgcaatggct
     9601 cccagtaaac tgccagcgat gcgtccgagg cttttgctga tgacaccgcc aacggtggga
     9661 aagctaacca ctgcagccga ggtcatcgcc caatagggtt catccagatt caaataatag
     9721 gcaaccgtca gcgccagaca catggcaatg gtattgcgta acgcataacg ccattgcgcc
     9781 agcgtggccc tgaaccagcg caaagcggac gggaacgcgt tgggcgagac gaacccaggg
     9841 gacgttaggt ttcacatccg gaataaggct tgagtcactt tcaacgctct gatcataaat
     9901 cgcccgaccg atactggaaa cgtgaccctg taacgttttg ttatcactat agagggtaat
     9961 ttgtgctggt gcaccttcgc ggatatggcg caacttggtt tcttcaaaat aaccaatgac
    10021 gtaaaacgag tggctgtcga caagggcaaa cagcggtttc ccggtgtcag cgtaatcgcc
    10081 gatgcgggta gtgaggttag tcacccatcc actcaccggg gcgcgaattt ccgtttgcgc
    10141 cagttgccat tgtgtctgtt tcagcgtcgc ctgtgcggca ttaacactgg cctgcatcgc
    10201 tttaacattg aggttagcgg tatcgagttc ttcggcaaaa ataaaatttt gtgagagatg
    10261 acgacgacga ttcgcttcgt tattggcttt tgcgaggtca gattgcgctt tcgccagctg
    10321 agcctgtgcg ttcagctcgg cgatctgaaa gggcgtttta tcgatcgtga gcagaagttc
    10381 ccccgcgtta accagttggt tgtcttttat attcagctca acaatacgtc ccgacacctg
    10441 tggggtgata gaaacctgtt ctgcgtgtat tttcccgtcg cgtgtccacg gagattgcat
    10501 gtaataattc cacagccacc atccggcaag aaccgcaact atcgctacaa tgattgttga
    10561 gaaatactta attgttttca ttgacatagt taccacgcaa tcagtattgc cagagcaagg
    10621 caaacgcaaa tcgcaaacag cgataaatcc attaacaagg gatgccagat gtcaccggcg
    10681 tagatccagc cacgaagcag acggtgtacg acaagccaga tgacgaatcc aaacgcgaat
    10741 gctttgaaaa acggaggaaa atagacggac gcaccgaaca ccagatcttg taagggcaat
    10801 cctgttgcat tgagcataaa cttcacaaag tagattcctt tacgaccgaa tatattgcgt
    10861 tagattaata aatattcttt aagtgcgaaa aatttacgcg caatttctga aaaatatgtc
    10921 tgacggtaac caaatgcagc aatacatttg ttttagcaat acaattgctg cacactattc
    10981 taaaagccgc ataatatctt agcaagctaa ttataaggag atgaaattgg aatcgccact
    11041 aggttctgat ctggcacggt tggtgcgcat atggcgtgct ctgatagacc atcgcctgaa
    11101 accgctggag ttaacacaaa cccattgggt tacgttacac aatatccatc agttacctcc
    11161 agaccagtcg caaattcaac tggcaaaagc gattggcatc gagcagccat cactggtccg
    11221 tactctggac caactggaag aaaaagggtt aatttcgcgt caaacttgtg ccagcgatcg
    11281 tcgggctaaa cgtattaaac tgacggaaaa ggcagagcca ctgatcagcg aaatggaagc
    11341 tgttattaac aaaacccgcg cggaaatatt acatggcatc tccgcagagg aactggagca
    11401 actgattacg ctcatcgcaa aacttgagca taatatcatt gagttacagg ccaaagggtg
    11461 aaatgaaggg ggcgtgtggt cagtaacctg accacacgca aacttattta gcgcggagaa
    11521 acggtcacct gactgccatt gctggccagt acgacacgtt ggcccggaga gaaacgagtg
    11581 ttgccttgtt tctgtaccac catgatggta ttaccatcgt ctttacgaat ttccagctcg
    11641 acaccctgcg ttttgttcat tgcactctgt acgccctgac cagctacgcc acctgcaaca
    11701 gcgcctgctg cagtagccag agaacgcccg gttccgccac caacagtatt ccccaggaaa
    11761 ccaccaagaa cagcaccgcc aattgcaccg ataacgttgg aatcatcacc gccctgaatc
    11821 tgtaccggac gtacgttaac gatggtgcca tagctgacat tctgtacttg tttcgcttca
    11881 gaagcggtat aaacatcccc tgacagggtg tcgttattaa cacaaccgac aagagacaga
    11941 cctaccattg aaacaaccaa tacgcgttta atcactgaaa aatctcctgt tcaccatgaa
    12001 acgccacgca agcatccctc atggtcaaag tatatggcat atgtgaatcc atggttacat
    12061 gttctaccaa atatgagcaa atcataatga aagtaggatc aaccaattat aaacaaaaca
    12121 ttcatgatga ttattgattt gaagataatg ttttactacc aacggtaaaa atcggtcatt
    12181 ccgcatggta ttcacgaaaa gtttatgttt gagtggtgcg caaacgtcag attgaaaaag
    12241 gatgaactat gaaatcgggc cgctttattg gcgttatgtc aggcaccagc cttgatggtg
    12301 ttgatgttgt gttggcgaca attgatgaac accgggtcgc acagctggca agcttgagct
    12361 ggccgatccc ggtgtctctg aaacaggctg tactggatat ttgccagggc cagcagctta
    12421 cactttcgca gtttggacag cttgatactc aactcgggcg actttttgct gatgcggtca
    12481 atgccttgct taaggaacaa aacctgcagg cgagagatat agttgcgatc ggctgtcacg
    12541 gtcaaaccgt ctggcatgaa ccgacgggcg tggcaccaca cactttacag attggcgata
    12601 acaatcaaat tgtggcacgc accggaatta cggttgtcgg tgattttcgc cgtcgcgata
    12661 ttgccttggg aggacaaggc gcaccgctgg tacctgcgtt ccatcatgcc ttgctggctc
    12721 acccaaccga gcgacgaatg gtgctcaata ttggcggcat cgccaatctg tcactgctca
    12781 ttcctgggca gccggttggc ggctacgata ccggtcctgg taacatgctg atggatgcct
    12841 ggatctggcg tcaggcaggt aaaccttacg ataaagatgc cgagtgggca cgggcgggta
    12901 aagttattct cccactgctg caaaatatgc tcagcgaccc gtatttctcg caacctgcac
    12961 cgaaaagcac cggacgcgaa tactttaact acggctggct ggagcgccat ttacgccatt
    13021 ttccgggtgt tgatccccgt gatgttcagg ccacactggc agaactcacc gccgtgacca
    13081 tttctgaaca agttttgttg agcggtggct gcgaacgatt gatggtatgt ggtggaggta
    13141 gtcgtaatcc gctactcatg gcgcgtctgg cggtattact gctaggcaca gaagtcacca
    13201 ccaccgatgc cgttggcatt agtggcgatg acatggaagc attggctttc gcctggcttg
    13261 cctggcggac gctggcggga ttaccaggaa atctgccttc cgtcactggc gcaagccagg
    13321 agacggtact gggggctatt ttccccgcca actcgtgata tcagaattaa ctgaattttt
    13381 tcttatcgtg tctgccgtta cacttggaag attaaggagg gtatttgccc tccggaccag
    13441 gaaagtcttc gggatatgac tatgaaaaaa ctgttaatta tcattttgcc ggtccttctc
    13501 tccggttgta gcgcctttaa tcaactggtt gagcgaatgc aaaccgatac gctggaatac
    13561 cagtgtgatg aaaaaccgtt gacggtcaaa ctgaataatc cgcgccagga agtcagtttt
    13621 gtttacgata atcaactact gcatctcaaa cagggccttt cagcctctgg cgcgcgttac
    13681 tccgacggga tctatgtttt ctggtcgaaa ggtgaggaag cgactgtcta taaacgcgac
    13741 cgcatcgtct tgaataactg tcagttacaa aatccacagc gttgagattt ttccaggggc
    13801 ggcgcacaat agcgtcaccc actgacaatc cgtaaagaaa accatgtctg ataacgacga
    13861 attgcagcaa atcgcgcatc tgcgccgtga atacaccaaa ggcgggttac gccgccgcga
    13921 tcttcccgcc gatccattaa ccctttttga acgttggctc tctcaggctt gtgaagccaa
    13981 actggcggac cctaccgcga tggtggtcgc taccgtggat gaacatggtc agccttatca
    14041 gcgcatcgtt ttactcaaac attacgacga aaaaggcatg gtgttttaca ccaacctcgg
    14101 tagccgtaaa gcacatcaaa ttgaaaataa tccgcgcgtt agcctgctgt tcccgtggca
    14161 tacccttgaa cgccaggtta tggtgatcgg taaagcagaa cgactttcga ctctcgaagt
    14221 gatgaaatat tttcatagcc gcccgcgtga tagccagatt ggtgcatggg tttcgaagca
    14281 gtccagtcgc atttctgccc gcggtatcct tgaaagtaaa ttcctggagc tgaagcagaa
    14341 gtttcaacag ggcgaagtgc cattgccgag cttttggggc ggttttcgcg tcagccttga
    14401 acagattgag ttctggcagg gcggtgagca tcgcctgcat gaccgctttt tgtaccagcg
    14461 tgaaaatgat gcgtggaaga ttgatcgtct tgcaccctga aaagatgcaa aaatcttgct
    14521 ttaatcgctg gtactcctga ttctggcact ttattctatg tctctttcgc atctggcgaa
    14581 aagtcgtgta ccggcaaagg tgcagtcgtt atatacatgg agattttgat ggcaagcagt
    14641 aacttgatta aacaattgca agagcggggg ctggtagccc aggtgacgga cgaggaagcg
    14701 ttagcagagc gactggcgca aggcccgatc gcgctctatt gcggcttcga tcctaccgct
    14761 gacagcttgc atttggggca tcttgttcca ttgttatgcc tgaaacgctt ccagcaggcg
    14821 ggccacaagc cggttgcgct ggtaggcggc gcgacgggtc tgattggcga cccgagcttc
    14881 aaagctgccg agcgtaagct gaacaccgaa gaaactgttc aggagtgggt ggacaaaatc
    14941 cgtaagcagg ttgccccgtt cctcgatttc gactgtggag aaaactctgc tatcgcggcg
    15001 aacaactatg actggttcgg caatatgaat gtgctgacct tcctgcgcga tattggcaaa
    15061 cacttctccg ttaaccagat gatcaacaaa gaagcggtta agcagcgtct caaccgtgaa
    15121 gatcagggga tttcgttcac tgagttttcc tacaacctgt tgcagggtta tgacttcgcc
    15181 tgtctgaaca aacagtacga tgtggtgctg caaattggtg gttctgacca gtggggtaac
    15241 atcacttctg gtatcgacct gacccgtcgt ctgcatcaga atcaggtgtt tggcctgacc
    15301 gttccgctga tcaccaaagc agacggcacc aaatttggta aaactgaagg cggcgcagtc
    15361 tggttggatc cgaagaaaac cagcccgtac aaattctacc agttctggat caacactgcg
    15421 gatgccgacg tttaccgctt cctgaagttc ttcaccttta tgagcattga agagatcaac
    15481 gccctagaag aagaagataa aaacagcggt aaagcaccgc gcgcccagta tgtactggcg
    15541 gagcaggtga ctcgtctggt tcacggtgaa gaaggtttac aggcggcaaa acgtattacc
    15601 gaatgcctgt tcagcggttc tttgagtgcg ctgagtgaag cggacttcga acagctggcg
    15661 caggacggcg taccgatggt tgagatggaa aagggcgcag acctgatgca ggcgctggtc
    15721 gattctgaac tgcaaccttc ccgtggtcag gcgcgtaaaa ctatcgcctc caatgccatc
    15781 accattaacg gtgaaaaaca gtccgatcct gaatacttct ttaaagaaga agatcgtctg
    15841 tttggtcgtt ttaccttact gcgtcgcggc aaaaagaatt actgtctgat ttgctggaaa
    15901 taatgcatta agtggaaagg gggagtgaga aatcactccc cctggttttt atacagggaa
    15961 catgatgaaa aatattctcg ctatccagtc tcacgttgtt tatggtcatg cgggtaacag
    16021 tgcggcagag tttccgatgc gccgcctggg cgcgaacgtc tggccgctga acaccgttca
    16081 attttctaat cacacccaat acggcaaatg gactggctgc gtgatgccgc ccagccattt
    16141 aaccgaaatt gtgcaaggca ttgccattgc cgccattgat aaattacaca cctgtgatgc
    16201 cgtattaagt ggctatctgg gatcggcgga gcagggtgaa catatcctcg gtatcgtccg
    16261 tcaggtgaaa gcagcgaatc cgcaggcgaa atatttttgc gatccggtaa tgggtcatcc
    16321 ggaaaaaggc tgtatcgttg caccgggtgt cgcagagttt catgtacggc acggtttgcc
    16381 tgccagcgat atcattgcgc cgaatctggt tgagctggaa atactctgtg agcatgcggt
    16441 aaataacgtc gaagaagcgg ttctggcagc gcgcgaactc attgagcaag ggccacaaat
    16501 tgtgttggtt aaacacctgg cgcgagctgg ctacagccgt gaccgttttg aaatgctgct
    16561 ggtcaccgcc gatgaagcct ggcatatcag ccgtccactg gtggattttg gtatgcgcca
    16621 gccggtaggt gttggtgatg tgacgagcgg tttactgctg gtgaaactgc ttcagggggc
    16681 aacgctgcag gaggccctgg aacatgtgac cgctgcagtc tacgaaatca tggtgaccac
    16741 caaagcaatg caggaatatg agctgcaagt ggtggctgct caggatcgta ttgccaaacc
    16801 agaacattac ttcagcgcaa caaagctctg aaatatctca tttcggccca caacgacctg
    16861 tgggccgatg gctattactt taagccttcc gctgacagcg cgtcttgtac ttccggacgt
    16921 tcagccatac gttgcataaa tgccgcaatg tgctctaacc cttccagatt cagtttcacc
    16981 gcgtatgccc agcgcagaac cgtaaacaga taggcatcag caattgtaaa tctttgcccg
    17041 cagatccagt gctcatcctt cagtgcctcg ttcacatatt gcagcttctt atccagctgc
    17101 gcgcgaactg tcggtttgta ctcttccggt gtatctgggc gaaacagagg tgtgaaacct
    17161 ttatgcagct cggtggcgat gtaattcagc cattcgatgg ttttatagcg ggaaatactg
    17221 tttaccggtg ccagcaactg gcggtcgggg acgctgtcgg caagatactg cataatcgca
    17281 acgccttccg tcagcaaagt accatcgtcc agcagcaatg caggcacctg ccccttaggg
    17341 ttaacggaaa agtaatcgtc accgttttcg agacgttttt tcattaaatc cacactgacg
    17401 agcgtaaaat cctttccgct ctcacgcagg gtaatatggg aagcgagaga gcaggcgccc
    17461 ggtttgtaga acaatttcat cggtaactcc ttttgtctgt ggtttaaagt atggtagtgc
    17521 gttgctgaac aaaaaaaaag ccgctaacga aaagttagcg gcttgtaaaa gagtttccct
    17581 gaattttacg ctacagtagc tttcgccgct ttgtctgcag cgtcatcctg cgtcatgcgg
    17641 tgcagtttag gcgcggtcag cagcatcagt actgcaataa cggcagtagc gacaccaatc
    17701 tgcaagaata cgcgaccata gacttccagt gacatcagcg ggtcggtaac gttatccggc
    17761 acagccatca tacccgcaac ataaccacca atcaggtttg caccggcagt ggtcaggaac
    17821 cagctaccca taatgaagcc catcagacgc tgcggaacga gttgtgcaac cattgccaga
    17881 cccagaccag agatcatcag ttccccgatg ctctgcaggc catagcttgc gaccagccag
    17941 ctgacagaca cgatacctgc gtcagacgcg aatttcgcac ccagcggcag aatcaggaac
    18001 gcaccagaac acatcaccat gccgattgca aacttggttg gcatcggcag ggtatcaccc
    18061 atcttgttat agatagcggc cagaatcgga ctaccgatga tgatccagaa cgggttcagt
    18121 gcctgatact gctcaggttc tacggccaga cccagaatgg agtgctcaac gttacgaatc
    18181 gcaaagaagt tcagtgacgt tggcatctgg ctgtacagca cgaagaagat gattgcttcg
    18241 agcatcagga tgaaggcaac gatcatttta cgacgcgcag cacctttcat cgcgaaagct
    18301 tctttaccga agataaccac aataccgaag gcaacaacgc ccagcgccat acgcgcaact
    18361 tcctgattgt gcagtagcca ggtggcaaca gcgatcagcg ccacaacacc aataatggtc
    18421 agcaacaggt tacggtagtt gactggttca aagtctggtt ttgaaccgta ctgtttaacc
    18481 cagcgttggc agaaggcgaa gttaacgata gtgatcagca ggcctacaac gctcaacgca
    18541 aacgcaacac tccagccgta tttcgcggcc agccacgggg tggctatcat agagaagaaa
    18601 gagccgatgt tgacggacat gtagtacatg gtgaatgcac cgtccagacg tgggtcgttt
    18661 ttctcatagc atgtagaaag cagagaagac gggttagctt taaacaggcc gttaccgacc
    18721 gcaatagccg ccatacccat ataaacaata ccggcgtcgt gaccagacca ggcaaccaga
    18781 gcataaccaa tcgccagcac aatagcgccg agcataatta cgcgtttagt acccagtacc
    18841 ttgtcaccta accagccgcc gatagcgacc agaccataaa ccagggcact aaaggaagag
    18901 aaaagggtga ttgaatccgc ttcagacata cccagttgtt taaccaggta aacagccata
    18961 attccttgta ggccgtaata accaaaacgt tcccataact caatcgagaa gatgagatag
    19021 aacgccttcg gttgtttgaa agcgttcaaa ctgacgcttt cagttggttt ttggtttgca
    19081 gtggacactt ttacctcttt ttttacatcc catattaacg ggggtgcttt ctcgcgttat
    19141 gtctaaaaaa catacgcgca attgttatag tgggagggga aacggcaggt aatgttcact
    19201 atcttgcact ttcagacaag tcctttgtaa taatctgtta catataactg aggcggggaa
    19261 ttcaaccatt ccgggaaatg ttatccagcg ttaaattttt caaaaaacgt aacggcagat
    19321 tttttcactg cattgagagg gggatgatgg ctttacggag atatgttctg ctatttagcg
    19381 tttttcctag tgagatagtc cagtttctga aaaatagcca gtgtaatgtt ttgcaggtca
    19441 atatttgttg cgtgtgtaaa tttaaatcat tttaacctga ttcttgcaat gtttttacat
    19501 tgccacagca tcgtgatctt gatcacgaat gaatagaaaa tttcgctatt aaaaaaacaa
    19561 ttaacctgca tttttggtta tccgcaaacg gattacctga tgataacgct aagcaatggc
    19621 atcattgacg ttaaaaagat gttttaaaag aggataaaga aaggttatca atggggtaat
    19681 cggtgttacc ccttttcttc agatgtcaac tttctctttg tattcacaaa gatcttcaat
    19741 aatacaagag ccacagcggg gcttgcgggc aatgcaggta taacgcccgt gcaggatcaa
    19801 ccaatggtgg cagtcgactt taaactctgc cggaaccact ttcagtagct tttcttctac
    19861 ctgttcgacg tttttccccg gcgcaaattg agtacgatta caaacgcgga aaatgtgcgt
    19921 gtcgacagca atagtcggcc agccgaatgc agtgtttaat acgacgttgg ctgttttacg
    19981 acctacgccg ggcagggctt caagcgcagc acgatcttcc ggaacctcgc cattatgttg
    20041 ttccagtaat atacggcagg ttttgatgat attttctgct ttgctgttat aaaggccaat
    20101 cgttttgata taggttttca ccccttcaac gcccagttca agcatcgctg caggcgtatt
    20161 cgccaccggg tagagtttcg ccgtcgcctt attaacactg atatcggtcg cctgagcgga
    20221 aagcagtacg gcaatcagca attcaaaagg cgaactgaaa ttaagctcgg tggtgggatg
    20281 aggattgttc tcacgcaggc gagtgaggat ctccagacgt tttgctttgt tcatcagaca
    20341 ttccctgttt caccgtttgg caatgcacgt tcggcagctg cttcagcacg gcgctttttc
    20401 attctttcat caatcaggta ttttcctgcc agcatcagtc ccaagccaat aaatgcacct
    20461 ggtggcagca tcgccagcag gaaaggggag tcggtgtgga aaatctccac gcgtaatact
    20521 tttgcccagc tacctaacag cgcatctgca ccgtcaaaca acgtgccatt gccgataatt
    20581 tcgcgtagtg aacccagcac gaacatggcg caggttgcgc ccgtaccaat tgaaaagccg
    20641 tccagtgccg aaagcgccgg accttttttg gcggcgaagg cttcagcgcg gcccacaacg
    20701 atacagttag tgacaatcag cggaataaaa atccctaatg attgatacag gccaaaggcg
    20761 taggcgttga tcagcatctg tacagcgctg accaccgagg cgatgatcat cacgtaaatg
    20821 ggaatgcgga tctcggctgg cgtccagtga cgcagcgtcg aaatggtcag gttggtcagc
    20881 gtcagtacca gcgtagtcgc aagtcctaaa cccagagcgt tagtggcagt ggacgtgacc
    20941 gccaatagcg gacaaaggcc gagcagttgg accagtgcag agttgttttt ccacaacccc
    21001 tgaacaataa cgtctttaat ttcgctcacg ctttattctc cacaggcagg aagttgagaa
    21061 agttgtgccg gtaacgtctg agcgtacaat ccggcgcgtt ttaccgcatt aaccaccgcg
    21121 cggggagtaa tcgtcgcgcc ggtgaactgg tcgaaatcac caccatcttt cttcaccgcc
    21181 cagtgcgcat catccgcacc actgattttt tttcccgcaa aatgggtgag ccagtcagaa
    21241 aggcgcaatt cgattttatc gccaagccct ggcgtttcgt ggtgctctgt cacgcgcgtg
    21301 ccaagtaccg tgccattaaa atcggctccc accagcagct gaatcgcacc ggaatagcca
    21361 tctggcgcgg ttgcttccag aacggcggct accggtttgt catcctgttt ggcgatgtaa
    21421 acccgatgct cacctttacc taactctggc gcagttacca gatagcaact ctgtgcgagc
    21481 gcattgttat agcgttcggc tggcagcacc tgaccaaata acgccttttg ttgcagactg
    21541 gcctgttcag caattgtcgt tttggtcatc tggttgatgg ccgcagttaa ccctgtagaa
    21601 cccgctgcaa atagtgccag tgtaatgccg tgttttcgga tagttttcag catggtttac
    21661 cctttgcgat ggccgtagac gcgcggacgc gtgtagtaat cgatcagagg aaccgtgatg
    21721 ttcgccagca ggacggcaaa agccacgccg tcaggatagc cgccgaaact gcggatcaac
    21781 cagactaata agcccgccag cgcgccgaac atcagccgac cacgattggt cgtagaagca
    21841 gtaactgggt cagtcaaaat aaagaatgcg ccgagcatag tcgcaccaga cagcagatga
    21901 atttgcggtg ctgccagtgt ttctggtgag aacaaccagc ccaacgttgc gcataacgcc
    21961 agcgttacta agaagctgag gggaatatgc cagcgaatcg ctttctgcca tagcaaccac
    22021 acgccaccag ccagccaggc gagatttacc cattgccaac cagcgcccgc cagaataccg
    22081 ctgtagatcg gatattgcat aatctgttca accgaatgac ctgcgcggac agaggttttg
    22141 aatgtatcca gcggtgtcgc ctgactaatg ccatcaatac ctaagcgtag cgtattcata
    22201 tcaccaccac tggcggtatg accgctgaaa ataacctgta tggcgtcgat aaaaccaggg
    22261 atgttgaccg caatttcatg tggcggtaac cagctggtca tctgcaccgg gaaggagatc
    22321 agtaagacca cataaccaat cattgccgga ttaaacgggt tttgccccag accgccatac
    22381 aactgtttag cgatgatcac cgcaaacacc gtacccagta cgaccatcca ccatggcgct
    22441 agggggggaa tacttaccgc cagcaataag cctgtcagca atgctgagtt atctttcaac
    22501 gttgcggcta ccgactgctt gcgtagtttg agtacgagag cttcggctaa cagagcactg
    22561 accgacgcca acaggatctg aacgagagta ccccaaccaa aaaaccacag ttgcgctgcg
    22621 attcctggca cggctgcgag caacaccaat agcataatgc gcgatgtctg gcgctggtta
    22681 tgggtataag gggagctagc tattctgaat accatttagt cctcgtttac aaccttttgc
    22741 tgggcggctt ttttggcctg aacgcgggca atagccgcgg caactgcggc tttgcgcgga
    22801 tcgacctgtt cttctggttc cgcattagcc tgttgctgtt ccagcttgcg tgctttggcg
    22861 cgggcaatag cggcttcgac ggcagctttg cgcggatcaa cctgttcttc tggctccgca
    22921 ttggcctgtt gctgttccag cttgcgtgct ttggcgcggg caatagcgg
//
LOCUS       DADLTF010000058        22785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.058, whole genome shotgun sequence.
ACCESSION   DADLTF010000058 DADLTF010000000
VERSION     DADLTF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.058"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..194)
                     /locus_tag="JN749_002098"
     CDS             complement(<1..194)
                     /locus_tag="JN749_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HAY7368514.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKGY"
     gene            complement(335..2368)
                     /gene="metG"
                     /locus_tag="JN749_002099"
     CDS             complement(335..2368)
                     /gene="metG"
                     /locus_tag="JN749_002099"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAY7368515.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2500..3609
                     /gene="apbC"
                     /locus_tag="JN749_002100"
     CDS             2500..3609
                     /gene="apbC"
                     /locus_tag="JN749_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HAY7368516.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3871..4152
                     /locus_tag="JN749_002101"
     CDS             3871..4152
                     /locus_tag="JN749_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HAY7368517.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4445..4987
                     /locus_tag="JN749_002102"
     CDS             4445..4987
                     /locus_tag="JN749_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7368518.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5068..5742
                     /locus_tag="JN749_002103"
     CDS             5068..5742
                     /locus_tag="JN749_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7368519.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            5758..8238
                     /locus_tag="JN749_002104"
     CDS             5758..8238
                     /locus_tag="JN749_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7368520.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            8254..9287
                     /locus_tag="JN749_002105"
                     /pseudo
     CDS             8254..9287
                     /locus_tag="JN749_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="JN749_002106"
     CDS             complement(9369..9707)
                     /gene="rcnB"
                     /locus_tag="JN749_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HAY7368521.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(9926..10768)
                     /locus_tag="JN749_002107"
     CDS             complement(9926..10768)
                     /locus_tag="JN749_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HAY7368522.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHEHHQDHEHHHDQGHHHHHEHGEYQDAHA
                     RAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVS
                     FSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFM
                     GIMR"
     gene            10889..11161
                     /gene="rcnR"
                     /locus_tag="JN749_002108"
     CDS             10889..11161
                     /gene="rcnR"
                     /locus_tag="JN749_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HAY7368523.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      11253..11349
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            11384..12172
                     /gene="thiM"
                     /locus_tag="JN749_002109"
     CDS             11384..12172
                     /gene="thiM"
                     /locus_tag="JN749_002109"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HAY7368524.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            12169..12969
                     /gene="thiD"
                     /locus_tag="JN749_002110"
     CDS             12169..12969
                     /gene="thiD"
                     /locus_tag="JN749_002110"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAY7368525.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            13034..13852
                     /locus_tag="JN749_002111"
     CDS             13034..13852
                     /locus_tag="JN749_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HAY7368526.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            13904..14650
                     /locus_tag="JN749_002112"
     CDS             13904..14650
                     /locus_tag="JN749_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7368527.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(14624..15589)
                     /locus_tag="JN749_002113"
     CDS             complement(14624..15589)
                     /locus_tag="JN749_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAY7368528.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(15586..16589)
                     /locus_tag="JN749_002114"
                     /pseudo
     CDS             complement(15586..16589)
                     /locus_tag="JN749_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(16586..17062)
                     /locus_tag="JN749_002115"
                     /pseudo
     CDS             complement(16586..17062)
                     /locus_tag="JN749_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            17216..18268
                     /gene="fbaB"
                     /locus_tag="JN749_002116"
     CDS             17216..18268
                     /gene="fbaB"
                     /locus_tag="JN749_002116"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HAY7368529.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            18576..19430
                     /gene="gatY"
                     /locus_tag="JN749_002117"
     CDS             18576..19430
                     /gene="gatY"
                     /locus_tag="JN749_002117"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HAY7368530.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            19459..20726
                     /gene="gatZ"
                     /locus_tag="JN749_002118"
                     /pseudo
     CDS             19459..20726
                     /gene="gatZ"
                     /locus_tag="JN749_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            20736..21188
                     /gene="gatA"
                     /locus_tag="JN749_002119"
     CDS             20736..21188
                     /gene="gatA"
                     /locus_tag="JN749_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HAY7368531.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            21219..21503
                     /gene="gatB"
                     /locus_tag="JN749_002120"
     CDS             21219..21503
                     /gene="gatB"
                     /locus_tag="JN749_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HAY7368532.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            21507..22785
                     /locus_tag="JN749_002121"
                     /pseudo
     CDS             21507..22785
                     /locus_tag="JN749_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
BASE COUNT         5905 a         5292 c         5752 g         5836 t
ORIGIN      
        1 tcatacccct tcttcacctt ctccgcaatc agcttcagtt ccgctttttc cgccgccgca
       61 gcatctgaaa aactttttat ctgactttgc cctttggtgc caacttttcc ccaactgata
      121 tgcaactcgt ttccctgttg ctccaccgtc cagaatttat gtgatttttc gtcctgatag
      181 ataaagtgtc tcattctgca tccctgtaat tttatccagt tgaatgcaga tgctaccagt
      241 atttatacgg gttagagaga gacaaaatcg caagaaaaca actttaggaa tttataaaga
      301 aaaggcgctg tcgatgcagc gccttgaagg gggattattt cacctgatga cccggtttag
      361 caccggcatc cgggcttagc aggaaaatat ctttcccgcc aggaccggca gccatcacca
      421 tgccttcaga gataccgaag cgcattttac gtggtgccag gttagccacc ataatggtgt
      481 gacgaccaat cagtgcctgc ggatccgggt aagcagaacg aataccggag aagacattgc
      541 gtttttcacc gccgagatcc agcgtcaggc gcagcagttt gtcagaacct tcaacaaact
      601 ctgcgttttc gatcagcgcc acgcgcaggt caactttggc gaagtcgtca aaggtgatgg
      661 tttcctgaat cggatcatct gccagtgggc cagtaactgg cgcggtagtg gctttcactt
      721 cttctttaga ggcttccacc agagcttcaa cctgtttcat atcgatgcgg ttatacagcg
      781 ccttgaacgg attcactttg tggcccagca gcggttgctg gataccatcc caggtcagtt
      841 ccgtattgag gaaggcttct gcacgctcgg tcagtttcgg cagtaccggc ttcaggtaag
      901 tcatcagcac gcggaacagg ttgatgccca ttgagcaaat cgcctgcagg tcagcatcgc
      961 gaccttcctg ttttgcgact acccacggag cctgctcgtc gacatagcgg ttagccaggt
     1021 cagccagtgc catgatttcg cgcactgctt taccaaattc acggctttcc cacgcttcac
     1081 caatcacttc agcggcatcg gtaaaggttt tgtacagctc agggtcagcc agctcgcttg
     1141 ccagcacacc gtcaaaacgc ttgttgataa agcccgcatt acgggaggcc aggttaacca
     1201 ctttgttcac gatatcggca ttcacacgct gaacgaaatc ttccaggttg agatcgatat
     1261 catcaatgcg cgaagagagt ttcgcagtgt agtagtaacg caggctgtca gcgtcaaaat
     1321 gattcagcca ggtgctggct ttaataaagg tgccgcgaga cttggacatc ttcgcgccgt
     1381 tcaccgtcac atagccatga acaaacaggt tggtcggctt gcggaagttg ctgccttcca
     1441 gcatggcagg ccagaacagg ctgtggaagt aaacgatatc tttaccgatg aagtggtaca
     1501 gctcggcggt ggagtctttc ttccagtatt catcgaagct tacgctgtcg ccgcgcttgt
     1561 cgcacagatt cttgaaagaa cccatgtagc caatcggtgc gtccagccag acgtagaaat
     1621 atttgcccgg cgcgttcgga atttcaaaac caaagtatgg cgcgtcgcgg gagatatccc
     1681 actgttgcag gccggattca aaccactcct gcattttgtt tgccacctgc tcctgcaacg
     1741 caccgctgcg ggtccatgcc tgcagcattt cgctgaaaga gggcagatca aagaagaagt
     1801 gttcagaatc acgcatcact ggtgtagcac cagaaaccac cgatttcggc tcgatcaatt
     1861 cagtcgggct gtaggtcgcg ccgcagactt cgcagttatc gccgtattga tccggggatt
     1921 tacatttcgg gcaggtgcct ttcacaaaac ggtccggcag gaacatgcct ttttccggat
     1981 cgtacagctg agagatggtg cggtttttaa taaaaccgtt ttctttcagg cgagagtaga
     2041 taagttctga caactggcgg ttctcttcgc tgtgcgtcga gtgatagttg tcatagctga
     2101 tgttaaagcc tgcgaaatca gtctgatgct cctgactcat ttcgccaatc atctgctccg
     2161 gggtgatacc gagctgctga gctttcagca tgatcggtgt accgtgggca tcgtcggcgc
     2221 agatgaagtt gacctcgtgg ccgcgcattc gctggtaacg gacccagaca tcagcctgga
     2281 tgtgctccag catatggccg aggtggattg agccgttagc gtacggcagt gcgcacgtca
     2341 ccagaatttt cttcgcgact tgagtcatag taggcattac ttcttaatag tgaaaagggg
     2401 ttagatagta ccaaatggga aaatgttaag taagtacttc tctatattct tcaggtaaac
     2461 tatcagctga tggtttatct atacaaaaaa ggagtcggga tgaacgaaca atcccaggcc
     2521 aaatcaccag aagccttgcg cgcaatggtc gccgggacac tggccaattt tcagcaccca
     2581 accctgaagc ataacctcac cacgctgaaa gcgttgcacc atgtcgcctg gatggacgac
     2641 acgttgcatg tggaactggt tatgccgttt gtctggcata gcgcgttcga agagttaaaa
     2701 gagcaatgta gcgccgaact gctgcgcatc accggggcga aagctatcga ctggaaactg
     2761 tcgcataaca tagccactct gaaacgcgtg aagaatcagc cgggaattaa cggtgtgaag
     2821 aacattatcg cggtgagttc cggtaaaggc ggcgtgggta aatcgtccac ggcggtaaac
     2881 ctggcgctgg cactggcggc ggaaggggcg aaagtcggca ttctggacgc cgatatctac
     2941 ggcccatcaa ttcccaccat gctgggcgca gaaaatcagc gtccaacttc gccggacggt
     3001 acccacatgg caccgattat gtctcatggc ctggcaacca attcaattgg ttatctggtc
     3061 actgacgaca atgcgatggt gtggcgtgga ccgatggcga gcaaggcgtt aatgcagatg
     3121 ttgcaggaaa ccttgtggcc ggatctcgac tatctggtgc ttgatatgcc gccaggcacc
     3181 ggtgacattc aattgacgct ggcgcaaaac attccagtaa ctggtgcagt cgtggtcacc
     3241 accccgcagg acatcgcgtt gatcgatgcg aaaaaaggca ttgtgatgtt cgagaaagtc
     3301 gaggttccgg tgctcggtat tgtcgagaac atgagtgtgc atatttgcag taactgtggt
     3361 caccatgaac cgattttcgg taccggtggt gcagagaaac tggctgaaaa ataccataca
     3421 cagctgctgg ggcagatgcc gttacacatc tccttacgtg aggatctcga taaaggcacg
     3481 ccaacggtta tcagccgtcc ggagagcgaa tttaccgcta tctaccgcca gctggctgac
     3541 cgcgttgcag ctcagctcta ctggcagggt gaagtcattc ctggcgagat ttccttccgc
     3601 gcggtctaac gcctattaac cattctggtt attcttcata catcgcagag atgttatatt
     3661 gatgcggtgt atttgttttt gccgcattaa tgaacactta tttaatactg cgaatattct
     3721 gcggaccttc gtccgaaact ctacaaagtc gagtgatcgt acaggcaatg cttcattatc
     3781 atccttcgtg ctgtgtagtg gatgtacagc tatagtttgt tatgcactca ttctatccag
     3841 gcataacctt tactacaggg ataacgttca atgaataagt attggctgtc aggaattatt
     3901 tttcttgcct atggattggc ttctcccgca gtctcttcag agactgccac attaacgatt
     3961 aatggcagga tcagtccccc aacatgcagt atggctatgg ttaatagtca accacaacag
     4021 cattgtggtc agttaacgta taatgttgac acccgtcatc aggtttcttc gcccgtcaaa
     4081 ggtgttacga cagaagtggt tgttgcaggt agcgatagca aacgtcgaat cgtgttaaat
     4141 cgctacgatt aatttggcaa cctgatttta attgcattta gaaagacgaa atcatttata
     4201 taaatgattt cgtctttttt attgatatca acaataccat ttacataaat ctacattaat
     4261 taatagatgt gataaatatt taatattctt aagtatttgt tgtatttata ttgcattggt
     4321 taatttgttt taaatcaata aaacaatatg tttacattgc ggcagctttt ttgatgtgag
     4381 aaatttgttt ccaagagata cagactctta acaaccaaaa ataaatttta ttaaggaaca
     4441 tattatgaaa cgttcaatta ttgctgccgc tgtcttttct tcttttttta tgagcgctgg
     4501 agtatttgct gcagacgttg ataccggaac attaaccatt aaaggaaata ttgcagaatc
     4561 tccgtgtaaa ttcgaagcgg gtggtgattc agtaagtatt aatatgccga ctgtaccaac
     4621 cactgtcttt gaaggtaaag ctaaatattc tacctatgat gatgcagtcg gtgtaaccag
     4681 cagcatgtta aaaattagct gcccgaaaga agttgctggt gtaaaactct cgttgatcac
     4741 aaacgacaaa ataaccggta acgataaggc gatagccagt agcaacgata ccgtgggtta
     4801 ctatctctat ttaggtgata acagcgatgt cctagatgtt tctgcacctt ttaacattga
     4861 gagttataaa acagcagaag gtcaatatgc tattccgttt aaagcaaaat acctgaaact
     4921 gacagataac tcagtgcaat caggtgatgt gttatcttct ctggttatgc gtgtagcgca
     4981 ggattaataa ataaaattgc aagtttctcc aggtaataaa catggccgct atccccatgg
     5041 cggcttttta atttaagagg cattaagatg aaaggattat tatctttact cattttttct
     5101 atggtccttc ctgcacatgc cggaattgtt atctacggga cgcgcattat ttacccggca
     5161 gaaaataaag aagttatggt gcagttgatg aaccagggaa atcgttcttc gctgctgcag
     5221 gcgtggattg atgatggcga tacgtcgtta ccaccagaaa aaattcaggt tcctttcatg
     5281 ttaacgccac cagtggcaaa aataggggca aattcagggc agcaagtaaa aatcaaaatt
     5341 atgccgaata aactgcccac taataaagaa agcatttttt atctgaatgt tctggatatt
     5401 ccaccaaata gtccagagca agaaggcaag aatgcactga agtttgcgat gcaaaacaga
     5461 attaagttgt tttaccggcc agcgggtatt gctccggtaa ataaagcgac ctttaaaaag
     5521 ttactggtaa atcgcagtgg caatggattg gtgataaaaa atgactcagc taattgggtg
     5581 acgatttcgg atgtcaaagc taataatgtt aaagtcaatt atgaaactat tatgattgcc
     5641 cccttagaaa gtcagagtgt taatatcaaa agtaataatg caaataactg gtatctgacc
     5701 attatcaatg accacggcaa ctatattagt gacaaaattt aattgcagga gctgcctatg
     5761 ttgagaatga ccccacttgc atcagcaatc gtagcgttat tgctcggcat tgaagcttat
     5821 gcagctgaag aaacctttga tacccatttt atgataggtg gaatgaaaga ccagcaggtt
     5881 gcaaatattc gtcttgatga taatcaaccc ttaccggggc agtatgacat cgatatttat
     5941 gtcaataagc aatggcgcgg gaaatatgag attattgtta aagacaaccc gcaagaaaca
     6001 tgtttatcaa gagaagttat caagcggtta ggcattaata gcgataactt cgccagcggt
     6061 aagcaatgtt taacatttga gcaacttgtt cagggtggga gctatagctg ggatatcggg
     6121 gtttttcgtc tcgatttcag tgtcccgcag gcttgggtgg aagaactgga aagtggctat
     6181 gttccaccgg aaaactggga gcggggtatt aatgcgtttt atacttctta ttatgtgagt
     6241 cagtattaca gcgactataa agcgtcgggt aacaacaaga gtacatatgt acgttttaac
     6301 agcgggttaa atttactgga gtggcaactg cattctgatg ccagtttcag taaaacaaat
     6361 aacaatccag gggtgtggaa aagcaatacc ctgtatctgg aacgtggatt tgcccaattt
     6421 ctcggcacgc ttcgcgtggg tgatatgtac acatcaagcg atatttttga ttctgttcgc
     6481 ttcagcggtg tgcggttgtt tcgtgatatg cagatgttgc ctaactcgaa acaaaatttt
     6541 acgccacggg tgcaggggat tgctcagagt aacgcgttgg taactattga acagaatggt
     6601 tttgtggttt atcagaaaga ggttcctcct ggcccgtttg cgattacaga tttgcagttg
     6661 gccggtggtg gagcagatct tgatgttagc gtgaaagagg cggacggctc ggtaaccacc
     6721 tatctggtgc cttatgcagc ggtgccaaat atgctgcaac ccggcgtgtc gaaatatgat
     6781 tttgcggcgg gccgtagcca tattgaaggg gcgagcaagc aaagtgattt tgtccaggca
     6841 ggttatcagt atggttttaa taacttattg acgctgtatg gcggcacgat ggttgctaat
     6901 aattactatg cgtttaccct cggaacgggt tggaacacgc gcattggcgc aatttccgtc
     6961 gatgccacga agtcgcatag taaacaagac aacggcgatg tgtttgacgg gcaaagttat
     7021 caaattgcct acaacaaatt tgtgagccaa acgtcgacgc gttttggtct ggcggcctgg
     7081 cgttattcgt cgcgtgatta ccggacattt aacgatcacg tttgggcaaa caataaagat
     7141 aattatcgcc gtgatgaaaa cgatatctat gacattgccg attattacca gaacgatttt
     7201 ggccgtaaaa atagcttttc tgccaatatg agccagtcat tgccagaagg ctggggttct
     7261 gtgtccttaa gtacgttatg gcgagattac tgggggcgta gcggcagcag taaggattat
     7321 cagttgagtt attccaataa ctggcggcgg ataagctata ccctcgcggc aagccaggcg
     7381 tatggcgaga atcatcatga agagaaacgt tttaatattt ttatatcaat tccctgtgat
     7441 tggggtgatg acgttacgac gcctcgtcgg caaatatata tgtctaactc aacgacgttt
     7501 gatgatcagg gttttgcctc aaataatacg ggattatcag gaacagtagg gagtcgggat
     7561 cagttcaatt atggtgtcaa cctgagtcat caacatcagg gaaatgaaac gacagctggg
     7621 gcgaatttga cctggaacgc gccggttgcg acagtgaatg gcagttatag tcagtcgagt
     7681 acttatcgac agactggagc cagtgtttca gggggcattg tcgcctggtc gggtggcgtt
     7741 aatctggcga accgtctttc cgaaacgttt gctgtgatga atgcgccagg aattaaagat
     7801 gcttatgtca atgggcaaaa atatcgcaca acaaaccgta atggagtggt ggtatacgac
     7861 ggaatgacac cttatcggga aaatcacctg atgctggatg tgtcgcaaag cgatagcgaa
     7921 gcagaattac gtggcaaccg gaaaattgcc gccccttatc gcggcgcggt tgtactggtt
     7981 aattttgata ccgatcagcg caagccctgg tttataaaag cgttaagagc ggatggtcaa
     8041 ccattaacgt ttggttatga agtcaatgat atccatggtc ataatattgg cgttgtcggc
     8101 cagggaagcc agttatttat tcgcaccaat gaaataccgc catcggttaa tgtagcaatt
     8161 gataagcaac aaggactttc atgcacaatc accttcggta aagagattga tgaaagtaga
     8221 aattatattt gccagtaata gtcaggtggt tctatggaaa ttcgcataat gctatttata
     8281 ttaatgatga tggttatgcc tgtgagctat gcggcatgtt atagtgagtt atctgttcag
     8341 cacaatttgg ttgttcaggg gaattttgca cttactcaaa cacaaatagc gacatatgag
     8401 cataatttta atgattcgtc atgcgtgagt acaaatacta tcacccctat gagcccatcg
     8461 gatattattg ttggacttta taacgatacc attaaattaa atttacattt tgaatggacc
     8521 aataaaaaca acatcacgtt gtcaaataat cagagcagtt tcaccagtgg ttattcggta
     8581 acggtgacac ctgcggccag taatgcaaaa gtgaatattt ctgcgggggg cggtggttca
     8641 gtgatgatta atggtgttgc gacattatcc agtgcttcat catcgacacg cgggagtgcc
     8701 gcagtacaat ttctactgtg tttattaggt ggcaagtcat gggatgcatg tgtaaatagc
     8761 tacagaaatg cattggcaca aaatgcaggt gtctattcct tcaatctgac attgtcatac
     8821 aacccgataa ccacaacctg caaacaggac gatttattaa ttactttaga cagtattccc
     8881 gtttcacaat taccagccac aggtaacaaa gcaacaataa atagtaaaaa aagggatatt
     8941 attctgcgtt gtaaaaattt attaggtcaa caaaatcaaa catcacggaa aatgcaggtg
     9001 tatttatcaa gttctgactt gttaaccaac agcgacacaa tactgaaagg tgcggaagat
     9061 aatgcgtagg atttattctt gaaagtaatg gttcgccagt cacactttta aatatcacta
     9121 acagcagtaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa cttacagata
     9181 caacggtttc aattccgata acagccagtt actacgtcta cgatacaaac aaagttaaat
     9241 ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa caagcaagac
     9301 gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc atgatgaggg
     9361 ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat tttaccgtcg
     9421 gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc ggcatcaggc
     9481 gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg gtacatctct
     9541 gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact taactcatac
     9601 ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca ggcggcactg
     9661 gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa ctctccactt
     9721 tggggatctg taatatcagt cgcatatctt tcctgccata caatataact gacaagcgat
     9781 attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc cctaaaagca
     9841 tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct gggctgaaga
     9901 taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca tgtacgccca
     9961 tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt ttagcgagag
    10021 tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg ccaacgccta
    10081 cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt gcgcccagtg
    10141 tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg caggggataa
    10201 ggccaccggt taagccaaat aacaaaattt gccagttgat gacctctcta ccatcaaagc
    10261 gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg ccatgttcgt
    10321 gatggtgatg atgtccttgg tcgtggtgat gttcgtggtc ttgatgatgt tcgtgatgat
    10381 ggtgatgatg ctcataatca tgcccgtgca tattctccag ccagttgcgt tcgccgcgcc
    10441 aggtacgcca gaacatccag aacacggtgc caataatgat cactgcggaa atcagctgga
    10501 gccacggttc tgctgattga gcagtaaagc gcttactgat caccatcccg ccaaaggcaa
    10561 tcaaccagac caccgcggta tgcgaaatgg ttgctgccag tccgagcatc accgcctgtt
    10621 taatggtgcc tttgatggcg atgataaacg ccgccatcat cgtttttgag tgccctggtt
    10681 ccaggccatg aagcgcacca agtaagatgg cgctggggat gaagaaccag gcgtttcctt
    10741 gctgaagaag agttgtaaat tcggtcatga taataattct tagtattaat tcggcaatct
    10801 gattctactc ccccccagta cctgatacta ccccccagta gattaatagt gctatgattt
    10861 ttcatattct tgttaaccag gtgttgccat gtctcataca atccgtgata aacagaaact
    10921 gaaagcgcgt gccagtaaga ttcagggcca ggtcgtggcg ctcaagaaaa tgctcgacga
    10981 gccgcacgaa tgcgctgcag ttttacaaca gattgctgct atccgtggag cggtaaacgg
    11041 tctgatgcgg gaagtgatta aaggtcatct gacggaacac atcgttcacc agggggatga
    11101 gctaaaacgt gaagaagatc tggatgtcgt tctgaaggtg ctggattcat atatcaaata
    11161 atttattaac gcgattgtaa aactgccgtt tttcctcgtt tacaacgggt gcgctggaca
    11221 ttaccatcct cctctgcgat ttatcatcgc aaccaaacga ctcggggtgc ccttctgcgt
    11281 gaaggctgag aaatacccgt atcacctgat ctggataatg ccagcgtagg gaagtcacgg
    11341 accaccaggt cattgcttct tcacgttatg gcaggagcaa actatgcaag tcgacctgct
    11401 gagttcagcg caatctgcgc acgcgttaca cctttttcac caacattccc ctcttgtgca
    11461 ctgcatgacc aatgatgtgg tgcaaacctt taccgccaat accttgctgg cgctcggtgc
    11521 atcgccagcg atggttatcg aaaccgaaga ggccagtcag tttgcggcta tcgccagtgc
    11581 cttgttgatt aacgttggca cactgacgca gccacgcgca caggcgatgc gtgctgccgt
    11641 tgagcaagca aaaagctctc aaacacgctg gacgcttgat ccagtagcgg ttggtgcgct
    11701 cgattatcgc cgccattttt gtcatgaact tttatctttt aaaccggcag cgatacgtgg
    11761 taatgcttcg gaaatcatgg cattagctgg cattgctaat ggcggacggg gagtggatac
    11821 cactgacgcc gcagctaacg cgatacccgc tgcacaaaca ctggcacggg aaactggcgc
    11881 aatcgtcgtg gtcactggcg aggtggatta tgttaccgat ggacatcgta tcattggtat
    11941 tcacggtggc gatccgttaa tgaccaaagt ggtaggaact ggctgtgcat tatcggcggt
    12001 tgtcgctgcc tgctgtgcgt taccaggcga tacgctggaa aatgtcgcat ctgcctgtca
    12061 ctggatgaaa caagccggag aacgcgcagt cgccagaagc gaggggccag gcagttttgt
    12121 tccacatttc cttgatgcgc tctggcaatt gacgcaggag gtgcaggcat gaaacgaatt
    12181 aacgctctga cgattgccgg tactgatccg agtggtggtg cggggattca ggccgatctt
    12241 aaaaccttct cggcacttgg cgcttatggt tgctcagtta ttactgcact ggtggcgcaa
    12301 aatacccgtg gcgtacagtc ggtgtatcgc attgagcctg attttgtcgc cgcccagctc
    12361 gattcggtgt tcagcgatgt gcgaatcgat accactaaaa tcggtatgtt ggcggaaacc
    12421 gatattgttg aagcggtggc agaacggttg caacgttatc agatccaaaa cgtggtactc
    12481 gacaccgtta tgctggcaaa aagcggcgac ccgctgcttt caccttcggc ggttgctacg
    12541 ctgcgcagtc gattattgcc acaggtttca ttaataacgc caaacttgcc cgaagctgcc
    12601 gccttgctcg acgcgccaca cgcgcgcacc gaacaggaaa tgctggaaca agggcgatcg
    12661 ctgttggcga tgggctgtgg cgcagtgcta atgaaaggtg gtcatctgga tgatgagcaa
    12721 agcccggact ggctgtttac ccgcgagggt gaacaacggt ttaccgcacc gcgcattatg
    12781 accaaaaaca cccacggcac tggttgtaca ctctctgcgg cgttggctgc actacgcccg
    12841 cgccatacaa actgggctga caccgtacag gaggcaaaaa gctggctttc atcggcgtta
    12901 gcccaggccg acacgctgga agttggtcac ggtattggtc cggttcacca cttccacgcc
    12961 tggtggtgag caccgtacta tagctgcttt ggttattgtc agcctttggc actgacattc
    13021 agggatgaaa ccaatgcagc taagaattac cagtcgaaaa aaactcaccg ctctattatg
    13081 cgctcttggg ctaatttcca tcgttgcaat ttatccgcgt cagacggtga attttttcta
    13141 ttcgacagca gttcagatta cagactacat ccacttctac ggttatcgcc cggttaaatc
    13201 tttcgctatt cgcattcctg ccagttacac cattcacgga ttagatgttt cgcgctggca
    13261 ggagcgtatc gactggcagc gggtggcaaa aatgcgcgac aacgatatcc gcttacagtt
    13321 tgcttttatt aaggctacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
    13381 gcaattaagc cgcgaaaatg gcctgctgcg cggggcgtat cattatttct ccccgtcggt
    13441 atccgcttca gttcaggcgc gattatttct gcaaacggtg gatttctcac aaggcgattt
    13501 ccctgccgtg ctggacgtag aagaacgggg aaaattatcg gcaaaagaat tacgcaagcg
    13561 ggtaagtcag tggctaaaaa tggtcgaaaa acgcacggga aaaaagccga ttatttactc
    13621 aggagccgtt ttttatcaca ccaatctggc gggctatttc aatgaatatc cgtggtgggt
    13681 ggctcactat tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
    13741 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
    13801 cacggtggat gagttgcagg cattcgttga tgggattaaa gaaacgcctt aaataagaag
    13861 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
    13921 ccagctgatt gcccaactga acgagcgtat tttagcggcg gataacacgc cgctttatat
    13981 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
    14041 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
    14101 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
    14161 gatcaacaac atcttcgaat actcgttgaa agaagcgaga ggtttttctc agcaggtggt
    14221 attgcgcggg aaaaagcccg atacgctatg ggttaataag cgtgtcgtga aatgtcccga
    14281 agaagttgcg cagcagctgg cggtcgaagc gggaagtgat gtctttttgc ttaagcgtat
    14341 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
    14401 tcatgatgtt gatgccatcg gaatttctct ttatgactat ttccgcagcc agcacattta
    14461 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
    14521 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
    14581 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
    14641 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
    14701 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
    14761 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
    14821 atgcccgaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
    14881 gaacgatcaa cggcgcggca aatttctcct gccattgctt gccaagtgtt gttatctctg
    14941 cagataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagt gacactaaag
    15001 gtcgacaggc catgatccgc gccagtaatg cgtccgggat atcgccaata cgtgggccaa
    15061 aatcgataaa cggcgtcacg tcttgcagct tttccagcca ttccactaac aattcgccgc
    15121 agggcgaggc cagttgataa ccggaaaaat agagcaggtt gccaggcgca acggttaatc
    15181 gtgccaacca ttggcgattc cattgatttt ccacaccgct gaatgacata aaagtgcgtt
    15241 cgccatccgg ctcaaccagt gccagacacc agccgttatc gctttcggcg ttatcgatca
    15301 ggctgattaa cccctctttc gccatccggt tgcgaataat ctccgcccac acgccctgac
    15361 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
    15421 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
    15481 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
    15541 gacgcgtggt taattcaggc agcagtgtgt gtaatcgagc gccgctcata ccgcctcccg
    15601 ttgttgacga tatttctcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
    15661 atccagctcc gcctttaacg caggatcgac agcgttaatg ccatgcaacg cgccgcaaat
    15721 cgccgtcgcc atagcaccta tggtgtctgt gtcgccgcca agattggcgc acaggacggc
    15781 gcagcggttt gggtcggttt gtgccagttc aactagcgca atggcgcacg gaacggactc
    15841 aatagtgctg gtgcctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
    15901 gtcggcatta cgcacaattt tcagcgcaat ctccagacgt gccgccagtg aggcgctgaa
    15961 cgtggtggtg cgtttttgct gtgcatgtcg cgcaattgaa ggcaatgaat caacaatcgc
    16021 tgaccagcgt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
    16081 aaccgccaga tcggatttat gggtcgggct ggacgccagc gccacatcat caataaagga
    16141 atcaacatca cgcgctggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
    16201 ggttacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
    16261 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca acgcccagtc
    16321 gagaatatta cgcccaatca gattcggatc gatcttgcct tcacattcca gtaacgcatc
    16381 cgccagacac aggccatcga ggtatcgtcg gtgaattcgg cgcggttaaa ataacaggcc
    16441 gcgttattct cctttggtcc aggcagaaaa cggtcaatcc agccaaagtg tgctttaacg
    16501 cggctgcgtg gccaaagctc ggagggcatc cccatcgcat cccctaacgc ctgcccataa
    16561 agagcaccga gaatacgttc tgttttcatt taacttcccc ttgtgtcaac gcaatatctc
    16621 gatcatcgac cttgatagcc gtaatttcgt tgtcggattc gcgggaaaaa atcatgaaca
    16681 gcacggcgat aatggcaatc atcaccgcgc cgaaagtcca catcccggac cagttgaaag
    16741 tcagtccgtt taccggttcc tgataagcga acatcttttc cgtcatcaca ccgccaagac
    16801 gatagccgag caaactgccg aagccctggc agcagagcgt gatcagcccc tgcgcagcgg
    16861 tacgcatatg cacgggggct tttttatcga catagatgta agcggtaacg tagtaaaaat
    16921 cgtaacttac gccgtgaagc aaaataccga ggaacagtaa cgcgtaggtg aaattgacag
    16981 gtaatgtaag gtacgcgatg acaataccac ataataatac cttttgaata cctttgcggt
    17041 aaaaacttga ccgcgctaac attttttctg atgaatcgag ccaacagaaa acgctgaaaa
    17101 aacatccaaa agatggaaaa actcgcccgt aacgaccagg attatgattg cccgcaaaag
    17161 gcgggccagg acaatccata gccgatatcc aatcggaatt tacgggagca tagtaatgac
    17221 agatattgcg cagttgcttg gcaaagacgc cgacaacctt ttacagcacc gttgtatgac
    17281 tattccttct gaccagcttt atctccccgg acatgactac gtagaccgcg tgatgattga
    17341 caataatcgc ccgccagcgg tgttacgtaa tatgcagacg ttgtacaaca ccgggcgtct
    17401 ggctggcaca ggatatcttt ctattctgcc ggttgaccag ggcgttgagc actctgccgg
    17461 agcttcattt gctgctaacc cgctctactt tgacccgaaa aacattgttg aactggcgat
    17521 cgaagcgggc tgtaactgtg tggcatcaac ttacggcgtg ttggtgtcgg tatcgcggcg
    17581 ctatgcgcat cgcattccat ttctcgtcaa acttaatcac aacgagacgc taagttaccc
    17641 gaatacctac gatcaaacgc tgtatgccag cgtggagcag gccttcaaca tgggcgcggt
    17701 ggcggttggt gcgactatct attttggctc ggaagagtca cgtcgccaga ttgaagaaat
    17761 ttctgcggcc tttgaacgcg cgcacgagct gggcatggtg gcagtgctgt gggcctattt
    17821 gcgaaactcc gcctttaaga aagatggcgt tgattaccat gtttccgccg acctgactgg
    17881 tcaggcgaac catctggcgg caaccatagg tgcagatatc gtcaaacaaa aaatggcgga
    17941 aaataacggc ggctataaag cgattaatta cggttatacc gacgatcgcg tgtacagcaa
    18001 gttaaccagc gaaaacccga ttgatctggt gcgttatcag ttagctaact gctatatggg
    18061 ccgggctggg ttgataaact ccggcggtgc tgcaggcggt gaaactgacc tcagcgatgc
    18121 agtgcgtact gcggttatca acaaacgcgc aggcggaatg gggctgattc ttggacgtaa
    18181 ggcgttcaag aaatcgatgg ctgacggcgt gaaactgatt aatgccgtgc aggacgttta
    18241 tctcgatagc aaaattacta ttgcctgata cgcctcatac gaacagcacc accccgtagg
    18301 acggataagg cgtttacact gcatccggca acctatgcct gatgcgacgc ggaagcgtct
    18361 tatcatgcct acatagcact gccacgtatg tttacaccgc atccggcata aaaacacgcg
    18421 cactttgcta cggcttccct atcgggaggc cgtttttttg cctttcactc ctcgaacaat
    18481 tttcatattg tcgtttttgt gatcgttatc tcgacattta aaagcaaata atttcattat
    18541 tttttgaaat cgaaagcaaa caacaagata tgaaaatgta cgtggtatcg acaaagcaga
    18601 tgctgaacaa cgcacagcgc ggcggttatg cggttccggc attcaatatt cacaatctcg
    18661 aaacgatgca agtggtggtg gaaaccgctg ccaacctgca tgcgccggtc atcatcgccg
    18721 gaacgcctgg cacatttact catgctggta cagaaaatct gttggcgctg gtcagcgcga
    18781 tggcgaagca atatcaccat ccactggcaa ttcatctcga ccatcacacg aaatttgacg
    18841 atatcgctca gaaggttcgt tctggcgtgc gctcagtcat gattgacgcc tcgcatttgc
    18901 cttttgcgca aaatatttca cgggtcaaag aggtggtgga tttttgccat cgctttgatg
    18961 tcagcgtcga ggcggagctg gggcaacttg gcggccagga agatgatgtg caagtcaatg
    19021 aagccgatgc gttgtacacc aaccccgcac aggcgcgtga atttgccgag gcaaccggga
    19081 ttgattccct ggcggtcgcc atcggcacgg ctcacggtat gtatgccagc gcaccggtgc
    19141 ttgatttttc tcgactggag aacattcgcc agtgggtgaa cttaccgctg gtgctgcatg
    19201 gcgcgtcagg attatcgact aaggatattc agcaaaccat caaactgggg atatgcaaaa
    19261 tcaacgttgc aacggagctg aaaaatgcct tctcgcagtc gttaaaaaat tacctgaccg
    19321 agcaccccga agcgaccgat ccccgggatt atttgcagtc ggctaaatcc gcaatgcgcg
    19381 atgtggtgag caaagtgatt gccgattgtg gctgcgaggg cagggcataa cgcacctgcc
    19441 atttaacaag gaaaaaacat gaaaacgtta attgcccggc ataaagctgg tgaacatatc
    19501 ggcatatgtt cagtctgttc tgcccatccg ttggttatcg aagcggcgct ggcatttgat
    19561 cgcaacagca cgcgcaaagt gctgattgaa gcaacgtcaa accaggtcaa tcaatttggc
    19621 ggttataccg gaatgacact ggcagacttt cgcgaatttg tttttacgat tgccgataaa
    19681 gttgggtttg cacgcgaacg cattattctc ggcggcgatc atctggggcc aaactgctgg
    19741 cagcaagaaa atgcggatgc ggcgatggaa aaatccgtcg agctggtaaa agcatatgtt
    19801 cgtgctggct tcagtaaaat tcatcttgat gcgtcaatgt cctgcgcggg ggatcccata
    19861 ccgttagcac cagaaacggt tgcggaacga gctgctgtgc tttgctttgc tttgctgcgg
    19921 aaagtgtggc gacagattgc cagcgtgagc aactgagcta tgtcattggc actgaagttc
    19981 cggttccggg cggtgaggcc agcgccattc agtcagtaca catcacccat gttgaagatg
    20041 ccgccaatac tttacgtacg catcaaaagg cctttattgc ccgtgggctg acagaggcgt
    20101 taacacgtgt gattgccatc gtggtgcagc cgggtgtgga atttgatcac agcaatatta
    20161 tccattatca gccgcaggaa gcgcaggcgc tggcgcaatg gatagaaaac acccgaatgg
    20221 tttatgaagc acattctacc gattaccaga cccggacggc ttattgggaa ttagtccgcg
    20281 atcactttgc aatattgaaa gtcggtcctg cattaacctt tgctttacgc gaggcgatat
    20341 ttgcactggc acaaattgag caggaactta tcgcccccga aaatcgcagc ggttgcctgg
    20401 cggtaattga agaagtgatg ctcgacgaac cgcaatactg gaaaaaatat tatcgcacgg
    20461 gttttaacga ttcattactg gatattcgtt acagcctgtc ggatcgtatt cgttattact
    20521 ggccgcatag tcggattaaa aatagcgttg aaacgatgat ggtgaatctg caaggcgtgg
    20581 acatcccact gggcatgatt agtcagtatc ttcccaaaca atttgaacgc attcagtccg
    20641 gggaattatc agcaatgccg catcagctta ttatgaataa aatttatgat gttttgcgcg
    20701 cctatcgcta cggttgtgcg gaataaggac ggtatatgac taacctgttt gttcgtagcg
    20761 gaatttcttt tgtcgatcgt agcgaagttt taacctatat cggtaatgag atgctcgcga
    20821 aaggtgtggt tcatgatacc tggccacagg cattaattgc cagagaagca gaattcccta
    20881 ccgggataat gcttgagcag cacgccattg caataccgca ttgtgaggcg attcatgcta
    20941 agtcgtcagc catttatctg ttaaggccaa caaataaagt tcattttcag caagcggatg
    21001 atgataacga cgtggcggta tcgttggtta ttgcgttgat tgtggaaaat ccgcagcagc
    21061 aattgaaact tttacgctgt ttatttggca agttacaaca gcccgatatc gtcgagacac
    21121 taattactct tcctgaaact cagttaaagg aatacttcac aaagtatgtt ttagattcag
    21181 acgaataaat ccctctgtaa caataaaaag gactatttat gaaacgcaag attattgtcg
    21241 cttgcggagg cgcggttgcg acctctacga tggcggcgga agaaattaaa gagttgtgtc
    21301 agagtcataa tattcctgtt gaattaatcc agtgtcgggt taatgaaata gaaacctata
    21361 tggatggtgt gcatttgata tgtaccactg ccagggtgga tcgtagtttt ggcgatattc
    21421 cgttagttca cggcatgcct tttgtttctg gtgtcggtat cgaagcatta caaaataaaa
    21481 ttctgactat cttacagggg tgacctatgt tttcagaagt catgcgttat attctcgacc
    21541 tcggccctac agtgatgctg ccggttgtca tcattatttt ttctaaaata ttaggcatga
    21601 aggcaggcga ttgctttaaa gcgggtctgc atatcgggat tggctttgtt ggcattggcc
    21661 ttgtgattgg cttaatgctg gattccattg gtccggcggc gaaagcgatg gcggaaaatt
    21721 tcgacctgaa tctgcatgtg gtcgatgtcg gctggccggg ctcttcacca atgacctgga
    21781 cgtcgcaaat tgcgctggtg gcgattccga ttgcgatatt ggttaacgtg gcgatgttac
    21841 tgacccgtat gacgcgggtg gtaaatgttg atatctggaa tatctggcat atgaccttca
    21901 ccggcagcgt tgctgcacct ggcaaccggt tcatggatga tagggatggc gggtgtggta
    21961 attcacgcgg cgtttgttta taagctcggc gactggtttg cccgcgatac ccgaaatttc
    22021 tttgagctgg aaggtattgc tattccgcac ggtacgtcgg cgtatatggg gccgattgcg
    22081 gtgctggtcg atgctatcat cgagaaaatc ccaggcgtta accgaattaa atttagcgcc
    22141 gacgatattc agcgcaaatt tggccctttt ggcgaacctg tcaccgtggg ttttgtgatg
    22201 gggctgatta tcggcatcct cgcgggttac gatgtcaaag gtgtattgca gctggcggta
    22261 aaaacggcgg cagttatgct gctgatgcca cggatgatta aacccatcat ggatggttta
    22321 acgcccatcg ctaagcaggc ccgtagtcgt ttacaggcga agttcggcgg tcaggagttc
    22381 ctgattggcc tggatcccgc attactgctg gggcatacgg cggtggtatc ggcaagcctg
    22441 atttttattc cgctcaccat tttaattgct gtttgtgttc cgggtaatca ggtactgccg
    22501 tttggcgatc ttgccactat cggcttcttc gtggcgatgg cggtcgcggt gcatcgtgga
    22561 aatctgttcc gcaccttaat ctcgggtgtc atcattatga gcatcaccct gtggatcgcg
    22621 acgcaaacta ttggtttgca cacccaactg gcggctaatg ctggggcgtt aaaagccggg
    22681 ggtatggtgg cttcaatgga tcagggcggt tctcccatta cctggttact gattcaggtt
    22741 ttctccccgc aaaatattcc cggtttcatt attatcggcg caatt
//
LOCUS       DADLTF010000059        22319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.059, whole genome shotgun sequence.
ACCESSION   DADLTF010000059 DADLTF010000000
VERSION     DADLTF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.059"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="JN749_002122"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="JN749_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HAY7368533.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /locus_tag="JN749_002123"
     CDS             831..971
                     /locus_tag="JN749_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368534.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="JN749_002124"
     CDS             985..1857
                     /locus_tag="JN749_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HAY7368535.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKNETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JN749_002125"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="JN749_002125"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAY7368536.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JN749_002126"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="JN749_002126"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HAY7368537.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JN749_002127"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="JN749_002127"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HAY7368538.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JN749_002128"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="JN749_002128"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HAY7368539.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JN749_002129"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="JN749_002129"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAY7368540.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="JN749_002130"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="JN749_002130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HAY7368541.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JN749_002131"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="JN749_002131"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7368542.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="JN749_002132"
     CDS             complement(14044..15384)
                     /locus_tag="JN749_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HAY7368543.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /locus_tag="JN749_002133"
     CDS             complement(15386..16738)
                     /locus_tag="JN749_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368544.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="JN749_002134"
     CDS             complement(17172..17621)
                     /locus_tag="JN749_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAY7368545.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JN749_002135"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="JN749_002135"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HAY7368546.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTVHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="JN749_002136"
     CDS             complement(18421..18750)
                     /locus_tag="JN749_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HAY7368547.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JN749_002137"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="JN749_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HAY7368548.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRMHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="JN749_002138"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="JN749_002138"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HAY7368549.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="JN749_002139"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="JN749_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HAY7368550.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5512 a         5916 c         5292 g         5599 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaaa tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccagg
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac agtttttcca cccggcccca gaaccacagt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtcg ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctt aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgatttcc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gaacagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttgg attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcatcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgcttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DADLTF010000060        21830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.060, whole genome shotgun sequence.
ACCESSION   DADLTF010000060 DADLTF010000000
VERSION     DADLTF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.060"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..157
                     /locus_tag="JN749_002140"
     CDS             <1..157
                     /locus_tag="JN749_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7368551.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="JN749_002141"
     CDS             227..1033
                     /locus_tag="JN749_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7368552.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="JN749_002142"
     CDS             1043..1450
                     /locus_tag="JN749_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HAY7368553.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="JN749_002143"
     CDS             1450..1944
                     /locus_tag="JN749_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HAY7368554.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="JN749_002144"
     CDS             2010..2807
                     /locus_tag="JN749_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7368555.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="JN749_002145"
     CDS             2818..3606
                     /locus_tag="JN749_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAY7368556.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /locus_tag="JN749_002146"
     CDS             3658..4887
                     /locus_tag="JN749_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7368557.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMELSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="JN749_002147"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="JN749_002147"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HAY7368558.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="JN749_002148"
     CDS             complement(6049..7680)
                     /locus_tag="JN749_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HAY7368559.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JN749_002149"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="JN749_002149"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAY7368560.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISSDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTMPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="JN749_002150"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="JN749_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HAY7368561.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /locus_tag="JN749_002151"
                     /pseudo
     CDS             12924..15108
                     /locus_tag="JN749_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JN749_002152"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="JN749_002152"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HAY7368562.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JN749_002153"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="JN749_002153"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HAY7368563.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGESMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JN749_002154"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="JN749_002154"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HAY7368564.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JN749_002155"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="JN749_002155"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HAY7368565.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21727
                     /locus_tag="JN749_002156"
                     /pseudo
     CDS             21287..21727
                     /locus_tag="JN749_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         5380 a         5590 c         5526 g         5334 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcggcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggagctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccgg atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttgctg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcatc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcatggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccgaagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc ccccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaattgaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ctttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac tttctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa cacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagc cggttgggtc
    21481 tatgttgtta agtgggcagc atatggatgc gctgtttatc gatcctgatg tgtgcggctg
    21541 cggcgtgggt cggatgctgg tggagcatgc gctctcgatg gcaccggaac tgacaaccaa
    21601 cgttaatgag caaaatgagc aggcggttgg gttctataag aaggtgggtt ttaaggttac
    21661 gggacgctct gaggtggacg atttggggaa accgtatccg ttgctgaatc tggcgtatgt
    21721 gggggataag ccgcccctgc cgtgcctgcc caagctggag aagacgcgcc agcgtcgcac
    21781 ccggcatttt tttcaccaac gcaaaaaggc catcctgacg gatagccttt
//
LOCUS       DADLTF010000061        21517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.061, whole genome shotgun sequence.
ACCESSION   DADLTF010000061 DADLTF010000000
VERSION     DADLTF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.061"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JN749_002157"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="JN749_002157"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HAY7368566.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="JN749_002158"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="JN749_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HAY7368567.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLHD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="JN749_002159"
     CDS             complement(2649..3200)
                     /locus_tag="JN749_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HAY7368568.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="JN749_002160"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="JN749_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HAY7368569.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="JN749_002161"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="JN749_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HAY7368570.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JN749_002162"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="JN749_002162"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HAY7368571.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JN749_002163"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="JN749_002163"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAY7368572.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JN749_002164"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="JN749_002164"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAY7368573.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JN749_002165"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="JN749_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JN749_002166"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="JN749_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HAY7368574.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="JN749_002167"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="JN749_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HAY7368575.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="JN749_002168"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="JN749_002168"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAY7368576.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JN749_002169"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="JN749_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HAY7368577.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JN749_002170"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="JN749_002170"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HAY7368578.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JN749_002171"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="JN749_002171"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HAY7368579.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSGNTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JN749_002172"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="JN749_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HAY7368580.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="JN749_002173"
     CDS             complement(18174..18569)
                     /locus_tag="JN749_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7368581.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JN749_002174"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="JN749_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7368582.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19484..20077
                     /locus_tag="JN749_002175"
     CDS             19484..20077
                     /locus_tag="JN749_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7368583.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20222..20674
                     /locus_tag="JN749_002176"
     CDS             20222..20674
                     /locus_tag="JN749_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7368584.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20797..>21517
                     /locus_tag="JN749_002177"
     CDS             20797..>21517
                     /locus_tag="JN749_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7368585.1"
                     /translation="MSKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5203 a         5330 c         5661 g         5323 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccaccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcacgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccaacgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggaggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tgccgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacagtttt gtaatggtta ctaaaaaaca
    19501 atctcgcgtt ccaggtcgtc ccagacgttt cgctcctgag caggcagtct ctgcggcaaa
    19561 agtgcttttt caccaaaaag gtttcgatgc agtaagtgtc tctgaagtta ctgattatct
    19621 tggtattaac cccccgagcc tctacgcggc ttttggcagt aaggctgggt tatttagccg
    19681 tgtactcaat gaatatgtcg gtacggaagc tattccgctt gccgatattc ttcgtgatga
    19741 tcgtccggta ggcgagtgcc tggttgaggt attaaaagaa gcggcgcgca gatatagcca
    19801 aaacggcggc tgcgctggct gtatggttct tgaaggtatt catagtcatg atccacaagc
    19861 gcgtgatatt gccattcaat attatcacgc cgcagaaacg accatttatg actatatcgc
    19921 caggcggcat ccacaaagcg cacaatgtgt gactgatttt atgagtaccg tgatgtcagg
    19981 gctttctgcg aaggcacggg aggggcactc catcgaacaa ctctgtgcaa cagctgccct
    20041 ggcgggggaa gcgataaaaa ctcttctcaa ggagtgatgc tggccttgat ccgaaaggcg
    20101 ggaacgcgcc tgccgataag ttgtgataag acaataattc acgcgttaag gctggcggaa
    20161 ttgatcatct tttcgtataa cgatagaaat gaaacattgt tttaattaag gagtggaaaa
    20221 gatgatcatc ggaaatattc ataatcttca gccgtggcta ccccaggagt tacgccaggc
    20281 gattgagcat atcaaagcac acgttacggc agaaacgcca aagggcaagc acgatatcga
    20341 aggcaatcgt ctgttttatc ttatctcgga agatatgacc gagccgtacg aagctcgccg
    20401 tgcggagtac catgcccgct atcttgacat tcagattgtg ttaaaaggtc aggaaggcat
    20461 gaccttcagc acgcaacctg caggcgcgcc ggataccgac tggttagctg ataaagacat
    20521 cgcatttttg ccggaaggcg ttgatgagaa aacagttatc ctaaatgaag gtgattttgt
    20581 tgtgttttat ccgggggaag tgcataaacc gctgtgcgca gtgggcgcgc cagcccaggt
    20641 tcgcaaagca gtagtgaaga tgctgatggc gtgatgactt ttcgccgtaa ataactccag
    20701 gtttacggcg agtttgtgaa aagagcgttt tttgatattt ttttgtgagt aaaatttgta
    20761 atgcttagac gttcttatta actcaaggag ttcgtcatga gcaaaatatc agatttgaat
    20821 tattctcaac acattacatt agccgacaat tttaaacaaa aaaatgaagc cttagatacc
    20881 tggtatgtgg gaatgaatga ttttgcccga attgccgggg ggcagaatag cagaagcaat
    20941 attctttctc ctagagcatt tttggagttt ttggcaaaga tatttaccct aggttatgtg
    21001 gattttagca aacgctccaa cgaagcgggt agaaatatga tggctcatat tgagttctca
    21061 tcttattcta aagatactga tggcaatgaa aaaatgaagt tttacatgaa taatcctgaa
    21121 ggggagcgag cggatttatc taaggtgaag atagaaatta cacttgcctc agcttctact
    21181 aaagggattc gtgaaggaca tacagtcatt atatttaaac aatctgatgg ttcgactaat
    21241 cgttatgaag ggaagtcttt tgaaagaaaa gatgatagtt cattacacct gattactaac
    21301 aaggttctgg cgtgttacca acgcgaagct aacaagaaaa tagcgcggct attaaataat
    21361 catcagaagt taaataatat acaggagtta aatgattcgc aggagttaaa taattcacag
    21421 aagttaaata attcgcagga gttaaataac tcgcaggtga gttgtaaagg ttcagttgat
    21481 tctacgatta cggatttatt agaaaaagca ttgaata
//
LOCUS       DADLTF010000062        21505 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.062, whole genome shotgun sequence.
ACCESSION   DADLTF010000062 DADLTF010000000
VERSION     DADLTF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.062"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JN749_002178"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="JN749_002178"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAY7368586.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JN749_002179"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="JN749_002179"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAY7368587.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="JN749_002180"
     CDS             complement(3121..3420)
                     /locus_tag="JN749_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368588.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /locus_tag="JN749_002181"
     CDS             3595..4590
                     /locus_tag="JN749_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7368589.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="JN749_002182"
     CDS             complement(4632..5069)
                     /locus_tag="JN749_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HAY7368590.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="JN749_002183"
     CDS             complement(5115..5456)
                     /locus_tag="JN749_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368591.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JN749_002184"
     CDS             complement(5726..7078)
                     /gene="xylB"
                     /locus_tag="JN749_002184"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HAY7368592.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGMWGQHWAQQGWRRLRRIQRNRSLSCCRSYR
                     "
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JN749_002185"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="JN749_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="JN749_002186"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="JN749_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7368593.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEREL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="JN749_002187"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="JN749_002187"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HAY7368594.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="JN749_002188"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="JN749_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HAY7368595.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13864
                     /locus_tag="JN749_002189"
                     /pseudo
     CDS             12686..13864
                     /locus_tag="JN749_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
     gene            complement(13972..14796)
                     /locus_tag="JN749_002190"
     CDS             complement(13972..14796)
                     /locus_tag="JN749_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HAY7368596.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="JN749_002191"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="JN749_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="JN749_002192"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="JN749_002192"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HAY7368597.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGETELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="JN749_002193"
     CDS             complement(18728..19201)
                     /locus_tag="JN749_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7368598.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JN749_002194"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="JN749_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HAY7368599.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="JN749_002195"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="JN749_002195"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HAY7368600.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21505
                     /locus_tag="JN749_002196"
     CDS             21362..>21505
                     /locus_tag="JN749_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HAY7368601.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5405 a         5487 c         5281 g         5332 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagag agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctagacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggca cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggcc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggga aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagacaa aaaagcgatc aaaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgcag ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aatatttatg cgcaa
//
LOCUS       DADLTF010000063        21504 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.063, whole genome shotgun sequence.
ACCESSION   DADLTF010000063 DADLTF010000000
VERSION     DADLTF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.063"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..2265
                     /locus_tag="JN749_002197"
                     /pseudo
     CDS             1..2265
                     /locus_tag="JN749_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            2266..3915
                     /locus_tag="JN749_002198"
     CDS             2266..3915
                     /locus_tag="JN749_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
                     /protein_id="HAY7368602.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3920..4696
                     /locus_tag="JN749_002199"
     CDS             3920..4696
                     /locus_tag="JN749_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HAY7368603.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDMDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            4971..7820
                     /gene="rcsC"
                     /locus_tag="JN749_002200"
     CDS             4971..7820
                     /gene="rcsC"
                     /locus_tag="JN749_002200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HAY7368604.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKKTLTVYAERVRKSRDS"
     gene            complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JN749_002201"
     CDS             complement(8020..8670)
                     /gene="rcsB"
                     /locus_tag="JN749_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HAY7368605.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JN749_002202"
     CDS             complement(8687..11359)
                     /gene="rcsD"
                     /locus_tag="JN749_002202"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HAY7368606.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQILIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            12097..13218
                     /gene="ompC"
                     /locus_tag="JN749_002203"
     CDS             12097..13218
                     /gene="ompC"
                     /locus_tag="JN749_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7368607.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            13330..14385
                     /gene="apbE"
                     /locus_tag="JN749_002204"
     CDS             13330..14385
                     /gene="apbE"
                     /locus_tag="JN749_002204"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HAY7368608.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            14459..15523
                     /gene="ada"
                     /locus_tag="JN749_002205"
     CDS             14459..15523
                     /gene="ada"
                     /locus_tag="JN749_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HAY7368609.1"
                     /translation="MKNATCLTDDQRWQSVLARDPDADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            15523..16117
                     /gene="alkB"
                     /locus_tag="JN749_002206"
                     /pseudo
     CDS             15523..16117
                     /gene="alkB"
                     /locus_tag="JN749_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            16193..17836
                     /gene="yojI"
                     /locus_tag="JN749_002207"
     CDS             16193..17836
                     /gene="yojI"
                     /locus_tag="JN749_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HAY7368610.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            18054..19678
                     /gene="mqo"
                     /locus_tag="JN749_002208"
                     /pseudo
     CDS             18054..19678
                     /gene="mqo"
                     /locus_tag="JN749_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(19828..20316)
                     /gene="eco"
                     /locus_tag="JN749_002209"
     CDS             complement(19828..20316)
                     /gene="eco"
                     /locus_tag="JN749_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HAY7368611.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            20724..21218
                     /gene="napF"
                     /locus_tag="JN749_002210"
     CDS             20724..21218
                     /gene="napF"
                     /locus_tag="JN749_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HAY7368612.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRVWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            21208..21471
                     /gene="napD"
                     /locus_tag="JN749_002211"
     CDS             21208..21471
                     /gene="napD"
                     /locus_tag="JN749_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HAY7368613.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
BASE COUNT         5210 a         5248 c         5764 g         5282 t
ORIGIN      
        1 attaacctcg tcgctgaaag gtaaacctgt ttctgcgcag ctaacggtag gcgtggtcga
       61 tgaaatgatc tacgcgctgc aaccagaaat cgcgccgaat atcggcaaat ttttctatcc
      121 gctggggcgt aacaatgtgc gtaccagctc cagtctgtcg tttatcagct atgaccaggc
      181 gctctccagc gagccggttg cgcctggcgc gactaaccgc agcgagcggc gagtaaaaat
      241 gcctgaacgt ccacggcgtg aagaggtgga taccgcggca tggatgccgt cactcacaac
      301 cgataaacaa ggcaaagcgt atttcacgtt cctgatgcct gattcgttaa cccgctggcg
      361 tatcaccgcg cgtgggatga acggcgacgg gctggtcggg caggggcgtg cttatctgcg
      421 ttcggaaaaa aatctctaca tgaagtggag tatgccaacg gtgtatcgcg tgggcgacaa
      481 accggcggca ggactgttta tcttcagtca gcaggataac gaaccggtgg cgctggtgac
      541 taaatttgca ggcgctgaga tgcgccagac gctgacgctg cacaaagggg cgaattatat
      601 ttcgctgacg cagaatattc agcaatctgg cttgttaagt gcagaactgc aacaaaatgg
      661 gcaagtgcag gacagcatta gcacaaaact gtcttttgtg gacaacagct ggcccgttga
      721 acagcagaaa aatgtcatgc tcggcggtgg cgagaacgcg ctgatgttgc ccgagcaggc
      781 gagtaatatc cggctacaaa gtagtgaaac gccgcaggag attttccgca acaatcttga
      841 tgcgttagtc gatgaaccgt ggggtggggt gatcaacacc ggtagccgtc tgatcccgct
      901 cagtctcgcc tggcgttcgc ttgccgatca tcaaagtgcc gccgctaacg acattcgtca
      961 gatgattcag gataaccgtc tgcggctgat gcaactggcg gggcccggag cgcgctttac
     1021 ctggtggggt gaagatggca atggtgacgc cttccttacg gcatgggcat ggtacgccga
     1081 ttggcaggcc agccaggcgc tcggcgtaac gcaacaaccg gaatactggc agcatatgct
     1141 cgacagttat gccgagcagg cggataacat gccgttattg catcgggcgc tggtgctggc
     1201 gtgggcacag gagatgaatc tgccgtgcaa aacgttgttg aaaggggggg atgaagctat
     1261 cgcccggcgc ggaactaaag atgaagattt ctctgaggaa gacatccgcg atatcaatga
     1321 cagcctgatc ctcgatacac cggaatctcc actggcagat gcggtggcaa acgtcttaac
     1381 catgacgttg ctgaaaaaag cgcagttgaa gtccacggtg atgccacagg ttcagcaata
     1441 tgcgtgggat aaagcggcaa acagcaatca gccgctggcg cacacggttg tgctgctcaa
     1501 tagcggtggt gacgctaccc agacggccgc tattttaatg gtttgaccgc tgagcaatcc
     1561 actattgagc gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctcca
     1621 gttgttttgc ctgcgcctgc gggcgcatgg gctaaacata agttaactgg agggggcgaa
     1681 gactggcgtt gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg
     1741 ccgcaaaatg tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt
     1801 ccggtgaccg ttgaacgcca gttgtatcgg cttatccccg gtgaagaaga gatgagcttt
     1861 actctgcagc cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatt
     1921 acgcttacct gcgagcagga tgcagttctg cgctatggtc aggtggaagt accgctcccg
     1981 ccgggagccg acgttgagcg cacaacatgg ggcatttcag tcaataaacc caacgccgcg
     2041 aaacagcagg ggcaattgct ggaaaaagcg cgtaatgaaa tgggcgaact ggcctatatg
     2101 gtgccggtga aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa
     2161 aaagggcaat tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcgcagcaa
     2221 agtgtcgcgg cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga
     2281 atcgtctggc tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa
     2341 ctggtgctgc gcggtgcgca gcacgatcaa ctgtatcagc tgtcgtcatc aggcgtgacc
     2401 aaagtctcag cattacctga ctcgctcacc acgccgttag ggagtttgtg gaaactgtat
     2461 gtttacgcct ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac
     2521 tccccggaag aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg
     2581 gtacgttcct gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg
     2641 gggcaatact ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag
     2701 ccagaaacct cggtaacggt taaatcgctg cttgattcat tagcgacgtt acctgcgcaa
     2761 aacaaagctc aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtctca
     2821 tccatgctcg gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag
     2881 gaaatccgtc agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact
     2941 ggcagtggca ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg
     3001 ccagtgccga cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat
     3061 ccgctgaaga aaatcacagc ggaaaagagt acgacggcag taaaacccag cgtacttaat
     3121 gggcgctatc gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag
     3181 acgaccttac tgagcgaaaa gggcaaactg aaattgcagt cgcatctcga tcgtgaagag
     3241 tacgttgcca gagttctgga tcgcgaggcg aaaagtacgc cacctgaggc agcgaaagcc
     3301 atgactgtgg cgatacgtac ttttttacag caaaacgcca atcgggaagg tgattgcctg
     3361 accatccccg gcagcagcgc cacgcagcgt gtttctgctt cgccagcgac cacgggggcg
     3421 cgaacgatga ccgcctggac gcaggagctc atctatgcag gcgatccggt tcattatcat
     3481 ggcagtcgcg ctaccgaagg gacactttcc tggcggcaag ccacggccca ggcggggcag
     3541 ggggagcgtt acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg
     3601 ggagctccac gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa
     3661 atgccgcagt ggcggcgtat attacaaggc gagacggggt acaacgaacc agacgtgttt
     3721 gcggtctgtc gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt
     3781 attcgtaact tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacttg
     3841 gcctttgatg gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa
     3901 ttattaatgg actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc
     3961 gcctgtcgct cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg
     4021 gcatccggca gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc
     4081 cgtcaacatg gcagatgatc aaaatatctc agcgcagatc agcggcaaaa taaagaatta
     4141 tgctgcggcg ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca
     4201 gagaattgaa tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag
     4261 cgtgcaggtc atcagcccgg atgggcaaag ccggcaaaaa atgcagtttt actcaacgcc
     4321 gggtacagga actattcgtg cacgtttacg gctggttctc tcgtgggata tggacaatac
     4381 cgatctcgat ctccatgttg tcacgcccga tggcgaacac gcctggtacg gtaacaccgt
     4441 gctgaaaaac agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt
     4501 cgccatgcca gcaccagttc acgggcgcta tcaggtgtat atcaactact atggtgggcg
     4561 cagcgaaacg gaattgacga ccgcccaact gacgctgatc accgatgaag gatcggtcaa
     4621 tgagaaacag gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa
     4681 aagttttgac tggtgatcca tcccttcaca caacctggct ctctcttttt tcagataaga
     4741 ctgctgggcg atctgccgac gccaggatat aaccattaac aagcgtcggc agattggtca
     4801 tttcttattt ttcgtactga gaactgtcca tctactccgg ctttcctggt taaggcaatg
     4861 gtttctaagc gatacgcatt attgcaatgg tgacagaaca ttgcggtaac acgcttttac
     4921 cgctacctta accacactcc atcggtcacc tgaggcggag cttcgcccct ttgaaatacc
     4981 ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat gttcagagca ttggcgttag
     5041 tgctctggct gttgattgct ttttcatccg ttttttacat cgttaatgcg ttacatcagc
     5101 gagaatcgga aattcgtcag gaatttaatc tgagttccga tcaggctcag cgctttattc
     5161 aacgcacctc tgatgtgatg aaagagctta agtacatcgc cgaaaatcgc ttatcggcag
     5221 aaaacggtgt gctttccccg cgtggacgag aaacgcaggc ggatgtgcct gcgtttgaac
     5281 cgctgtttgc cgactccgat tgttccgcaa tgagtaacac ctggcgaggt tctctggagt
     5341 cattggcgtg gtttatgcgc tactggcgcg ataatttttc tgcggcttac gatctcaacc
     5401 gggtattttt aatcggcagt gataacctct gcatggccaa tttcggtctg cgtgatatgc
     5461 cagtggaacg cgataccgcg ttgaaagcgt tgcatgaacg catcaataaa tatcgaaatg
     5521 caccacaaga tgatagcggc agtaacctct actggatcag cgaaggtccg cgccctggcg
     5581 tcgggtattt ttacgcgttg acgccagttt atctggcgaa ccggttgcag gcgcttttgg
     5641 gtgtcgagca gaccatccgg atggagaact ttttcttacc gggtacgttg ccgatggggg
     5701 ttaccattct tgatgaaaat ggtcataccc tgatttcgct taccggacca gaaagtaaaa
     5761 ttaagggcga tcctcgctgg atgcaggaac gctcctggtt tggctatacg gaagggttcc
     5821 gggagctggt gctgaagaaa aatctgccac cctcatcgct aagcatcgtg tattcggtgc
     5881 cggttgataa ggtgctggaa cgcattcgca tgttgatcct taacgcaatt ttgctgaatg
     5941 tgcttgccgg agctgcattg tttactctcg cgcggatgta cgagcgacgt attttcattc
     6001 cggcggaaag cgacgccctg cgactggaag aacatgagca gttcaatcgc aagattgtcg
     6061 cctccgcgcc agtgggtatc tgcattttgc gtaccgctga tggcgtcaat attttaagta
     6121 acgaactggc gcatacctat ctcaatatgc ttacgcatga ggaccgccaa cgactgacgc
     6181 aaattatctg tgggcagcag gtcaattttg ttgatgtcct gaccagcaac aataccaatc
     6241 tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt tgtgtgctgg
     6301 tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca ggagatggca caagcagcgg
     6361 aacaggcgag ccagtcaaaa tcgatgttcc ttgccaccgt cagtcatgag ctgcgaacgc
     6421 cgctgtatgg cattatcggt aacctggatc tgttgcaaac caaagagtta ccgaaaggcg
     6481 tcgatcgtct ggtgacggca atgaacaact cttccagcct gttgttgaaa attatcagcg
     6541 atattctcga tttctcgaag attgaatcgg aacagttgaa gatcgaaccg cgtgagtttt
     6601 caccgcgtga agtgatgaac cacatcaccg ccaactattt accgctggtg gtacgcaagc
     6661 agttaggctt gtactgcttt attgaaccgg atgtgccagt ggccttaaat ggcgacccga
     6721 tgcgtttaca gcaggtcatc tccaacctgt tgagtaacgc cataaaattc accgataccg
     6781 gctgtatagt tttgcatgtt cgcgctgatg gcgattatct ctctatccgt gttcgcgata
     6841 ccggcgtggg gattccggcg aaagaagtgg tgcgcttgtt tgatcccttc ttccaggttg
     6901 gaacgggcgt acagcgaaac ttccagggga ccggtctggg tctggcgatt tgtgaaaaac
     6961 tgatcagcat gatggacggc gatatctcgg tagattcaga accgggaatg ggcagccagt
     7021 ttaccgtgcg tattccgttg tacggcgctc agtacccgca gaaaaaaggc gtggaagggt
     7081 tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag ttcctggaaa
     7141 ccagtttgca gcgcagcggc atcgtcgtta caacatacga agggcaggaa ccgactcccg
     7201 aagatgtgtt aatcactgac gaggtagtga gtaaaaaatg gcagggcaga gcggtagtga
     7261 ccttctgtcg tcgccatatt ggtattccgc tggagaaagc gccaggggag tgggtacaca
     7321 gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg tatttatttg atcgagatgg
     7381 agagcgacga tcctgctaac gctctgccgt cgacggacaa agcggtcagc gataacgacg
     7441 atatgatgat tctggtcgtg gatgatcatc cgattaaccg acgtctgctg gcagatcagt
     7501 tgggatcgtt gggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg cttaatgtac
     7561 ttagtaagaa tcatattgat atcgtgctta gcgacgtcaa catgccaaat atggatggtt
     7621 accgcttgac gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc ggagtaactg
     7681 ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc cggtatggac agctgcctct
     7741 cgaagccggt aacgctggat gtgataaaaa agacgctgac ggtatatgcc gagagggtca
     7801 ggaaatcgcg ggattcgtag gacggataag gcgtttacgc cgcatccggc attcagtgca
     7861 aatgccagat aagacactga cgcgtcttat ctggcctact ttaatgtaat ctgttgaaat
     7921 aatgggaatc gtaggccgga taaggcgttt acgccgcatc cggcaatcgg tgcaaatgcc
     7981 agataagaca ctaacgcgtc ttatctggcc tacagatgat tagtctttat ctgccggact
     8041 taaggtcaca gaagagagat aattcagcag ggcgatatcg ttctcgacgc ccagcttcat
     8101 catcgcagat ttcttctggc tactgatggt tttaatactg cggttcagct ttttagcgat
     8161 ctcggtcact aggaggcctt ccgcaaacag gcgcagaact tcactctctt ttggcgagag
     8221 acgcttgtca ccgtaaccac cagcactgat tttttccaac aggcgagaaa cgctttccgg
     8281 ggtaaatttc ttccctttct gcagcgcggc gagagctttc ggcagatcgg tcggtgcacc
     8341 ttgtttcagc acgatccctt cgatatccag atccaatacc gcactaagaa tcgccgggtt
     8401 gttgttcata gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga tgtacttgat
     8461 taaggtaatg ccatcgccgt acttatcgcc aggcatggag agatcggtaa tcaacacatg
     8521 cgcatccagt ttcggcaggt tgttgatcag tgctgtagag tcttcaaatt cgccgacaac
     8581 attcacccac tcaatttgct caagtgattt gcgaataccg aacaagacta tcggatggtc
     8641 atcggcaata attacgttca tattgttcat gtaataggct accttgctac agcaagctct
     8701 tgacataact gtcaatgtcg ctgatgtatt tttctattct tggaacatcc ttctcacgaa
     8761 tcagatgttc cagcgtttca cataactgct tgccgggtac cagatttagc atggcaaata
     8821 cgcctttaag acgatgagcc gtttgggcta acgcagcaaa gtcactggtt gctgcttcag
     8881 tatacagcct cttaacatca tccggtactg tgtctacaaa gagcgcataa tagccgctgg
     8941 catggagttg cgcattttca tctccgccca gaggcgattc tgtcacctct tcctgcgcca
     9001 gttgcacttc aattaattgt aagaccgctt cctgcatagc gttgctcata ttgaagttga
     9061 cgcacaattg accaggccca atttctcgta cgccagactc atcatcgctt aaaagcaagc
     9121 cagaggcagt aagattagac ggattatccg ttaaaaagat atcataatct tgactaatta
     9181 atctttcatc gggtgtgata caggttgcac cccaattttc taacaggcga gtgacaatat
     9241 tccgaatttc tgccgaagta acatccacca ttacgcagac atcatccagt aaacgctctt
     9301 cttcctcttc aacttccggg tcagcggcga gcattttgat atgcacagag tagcgtgtac
     9361 caagcccatc ccgcgttttg atgtttaaat gaccgcccag tttacgtgcc agttgatcgc
     9421 tcagccagaa tgccagcggg tccgcctttc cataacgatc gttttgggtc tggttgataa
     9481 acgggaagtg caaattatcc atttcatgaa tacttacgcc ttctcccgtg tccagaatgc
     9541 ggaatgtcag gcggtcttca gaggactcat cctgatcaac ctcaagggtg atttttccca
     9601 attgcgttga ggtcacggca tattgcatca gtagcagcaa aatacgtcgt aaggcatcgc
     9661 gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt aatcagcagt tgcagacctt
     9721 tacgcttgat ggcaggcaac actgaaggca caacttcatc aattaaatcc tgcacggaga
     9781 acagcaccgt ctcacttttc cagctatcat ccgcaagcat gttcgctaac tgtatttctt
     9841 cgaccagccg caccagcaca tctgcctgat tcgccagttg tttgctttcc ggggcgttga
     9901 gtttagccgc actctccgcc agggactgtg cgggttcttt cagcgcatcg ccaatgtttt
     9961 tcataaaggt catccgcccc tgctggtttt tctcatacag acgctgcgcc tgcttgagtt
    10021 tcttgtttac cagcacttcg cgatcctgat cgcgaataat gagaatttgt gtgcgcggcg
    10081 cgacctggct gcggaacatg cggatctcat acagctcgtt attgatcgtc gcctgaataa
    10141 tcccctgatg ctgttccgcc atggtggtga tgttttgcag attcagatgc ggcagcaaat
    10201 gatcggcaat tttgttactt atgacagtgc ggttcgattc ctgatcgtga accagcaggc
    10261 cgagcggcaa cagtgagact atctcttcat taattgcccg taaaatgcgc aattcgttat
    10321 tgaccgccgt gctggggacg ctttctgtac tgcggctgga gaaatggcgg aatgtggtat
    10381 agccaaataa cgccagcgcc agcaaaccga tgttcagcag cagtggcagc agaatgtttt
    10441 gcaacgtatc cagcaataag gtgccataag gaacctgcca gaccaggcgc atatcggtag
    10501 agttgagtgc cgaggagatt tcaatcttcg tactgttaaa gtggatactg acgctatccg
    10561 tcccttcttt ctcattatca ttgtttcccg ttgccgtcgc gtctggctca aggcggaaac
    10621 tgtccagcgg catacccggt gggatcaaat cattaatcgg cagatcaaaa gccacgaccg
    10681 ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga ccgttctgcc
    10741 aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg gcctgttgca
    10801 acatttctgc acgacgtgaa tcaacaatgt cactgacggt cgattcttta aatccggagg
    10861 tgagatcttt aaggggtagg gttgagatca gcaccagact gttatcctga ccattcaggt
    10921 aatacatcga ccacggtaca ttttctgcac cccacaatgt atccagataa gtggacatcc
    10981 gctgagtcat ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct tccgttttgc
    11041 ggcgtggttt ctccagatag tagacatcct gtttcaggcg cgtctcttgt aaaccttcgc
    11101 cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat ctgcctggtc acgtaacgcc
    11161 aggtatcgac gcgcttttgg attgcgtggg taatgtcgac aatctggtaa cttttatctt
    11221 tcagccaggc gttaacggca ctttgtacca ttacccccat cgtcaccagt aacacaatga
    11281 tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg gtcgtggccg
    11341 ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa gtgatagggc
    11401 agtataaagg gtacagtgtg aaattccaga ctcttacctg attgatgatt ctctattctc
    11461 ccgtgatatt tatcatcctg tatgggggga aatagatgta caagcgccat tttgctgtac
    11521 aaataaccta caaaaagccc aacgaaatta attgtaaata aacaaagatt gctggaaatt
    11581 atgctgatgt taattatttg tgaaatagtt aacaagcgtt atagtttttc tgtggtagca
    11641 cagaataatg aaaagtgtgt aaagaagggt aaaaaaaccg aatgcgaggc atccggttga
    11701 aataggggta aacagacatt cagaaatgaa tgacggtaat aaataaagtt aatgatgata
    11761 gcgggagtta ttctagttgc gagtgaaggt tttgttttga cattcagtgc tgtcaaatac
    11821 ttaagaataa gttattgatt ttaaccttga attattattg cttgatgtta ggtgcttatt
    11881 tcgccattcc gcaataatct taaaaagttc ccttgtattt acattttgaa acatctgtag
    11941 cgataaatga aacatcttaa aagttttagt atcatattcg tgttggatta ttctgcattt
    12001 ttggggagaa tggacttgcc gactgattaa tgagggttaa tcagtatgca gtggcataaa
    12061 aaagcaaata aaggcatata acagagggtt aataacatga aagttaaagt actgtccctc
    12121 ctggtcccag ctctgctggt agcaggagca gcaaacgctg ctgaagttta caacaaagac
    12181 ggcaacaaat tagatctgta cggtaaagta gacggcctgc actatttctc tgacgacaag
    12241 tctgtagatg gcgaccagac ctacatgcgt cttggcttca aaggtgaaac tcaggttact
    12301 gaccagctga ccggttacgg ccagtgggaa tatcagatcc agggcaacag cgctgaaaac
    12361 gaaaacaact cctggacccg tgtggcattc gcaggtctga aattccagga tgtaggttct
    12421 ttcgactacg gtcgtaacta cggcgtagtt tacgacgtaa cttcctggac cgacgtactg
    12481 ccagaattcg gtggtgacac ttacggttct gacaacttca tgcagcagcg tggtaacggc
    12541 ttcgcaacct accgtagcac cgacttcttt ggtctggttg acggtctgaa cttcgctgtt
    12601 cagtatcagg gcaaaaacgg tagcccggaa ggtgaaggca tgaccaacaa cggtcgtgaa
    12661 gctctgcgtc agaacggtga cggcgttggc ggttctatta cttatgatta cgaaggcttc
    12721 ggtatcggtg ctgcagtttc cagctccaaa cgtactgatg accagaactt cggtctgaac
    12781 cgttatgatg agcgttacat cggtaatggc gaccgcgctg aaacctacac cggtggtctg
    12841 aaatacgacg cgaacaacat ctacctagct gctcagtaca cccagaccta caacgcaact
    12901 cgcgtaggca acttgggttg ggcgaacaaa gcacagaact tcgaagctgt tgctcagtac
    12961 cagttcgact tcggtctgcg tccgtcccta gcttacctgc agtctaaagg taaaaacctg
    13021 ggtgtcatca atggtcgtaa ctacgacgac gaagatatcc tgaaatatgt tgatgttggt
    13081 gcgacctact acttcaacaa aaacatgtcc acctacgttg actacaaaat caacctgctg
    13141 gacgacaacc agttcactcg tgacgctggc atcaacactg ataacatcgt agctctgggt
    13201 ctggtttacc agttctaatc tcgatggata tcgaacaaag ggcctgcggg cccttttttc
    13261 attgttttca gcgtacaaac acagtttttt ggtgtactct tgcgaccgtt cgcatgagga
    13321 taatcacgta tggaaataag ctttacccgc gtggcactgc tggttgccgc gctcttcttt
    13381 gttggttgcg atcaaaaacc acaacccgcc aaaacccacg ctactgaagt taccgttctt
    13441 gaaggcaaaa ccatgggtac cttctggcgt gccagcatcc cgggcattga tgccaaacgc
    13501 agtgccgaac ttaaagaaaa gattcagacc cagctggacg ccgacgatca gctgctttcg
    13561 acctataaaa aagattccgc gctgatgcgc tttaacgact cgcaaagttt gtcgccgtgg
    13621 ccggtaagtg aagcgatggc cgatatcgtc accacctcgc tgcgcattgg ggcaaggacc
    13681 gatggtgcga tggatattac cgtcgggccg ctggtgaatc tgtggggctt tggcccggaa
    13741 caacagccgg ttcaaattcc gagccaggaa cagatcgatg cgatgaaagc caaaaccggc
    13801 ttacagcacc tgacggtcat caatcagtcg catcagcaat atctgcaaaa agacctgccg
    13861 gatttatatg tcgatctctc caccgtcggt gaaggttatg cggcggatca cctggcacgc
    13921 ttgatggagc aggaagggat ttcccgctat ctggtgtcgg tgggcggcgc gctgaacagc
    13981 cgtggtatga acggtgaagg ccagccgtgg cgggtagcga ttcaaaaacc aaccgataaa
    14041 gaaaacgcgg ttcaggctgt ggtggatatc aacggtcatg ggattagcac ttctggcagc
    14101 taccgtaact attacgaact ggacggcaaa cgtctttccc atgttatcga tccgcaaacc
    14161 gggcgtccca tcgaacacaa tctggtatcc gtgacggtga ttgctccgac ggcgctggaa
    14221 gccgatgcct gggatactgg cttgatggta ctcgggccgg agaaagccaa agaggttgtt
    14281 cgccgggaag ggctggcggt ttatatgatc accaaagaag gcgatagctt taaaacctgg
    14341 atgtcaccac agtttaaaag cttccttgtc agcgaaaaaa attaaagcgc aagattgttg
    14401 gtttttgcgt gatggtgacc gggcagacta aaggctatcc ttaaccaggg agctgattat
    14461 gaaaaacgcc acatgcttaa ctgacgatca acgctggcaa tctgtcttag cccgcgaccc
    14521 ggatgccgac ggcgaattcg ttttcgccgt gcgcaccacg ggtatctttt gccgtccgtc
    14581 ttgccgcgcc agacatgcct tgcgggaaaa tgtctccttc tacgcaaatg ccagcgaggc
    14641 actcgccgcc ggatttcgcc cctgcaaacg ttgtcagcca gacaaagcca atccccggca
    14701 acatcggttg gataaaatca cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac
    14761 gctggaagcc ttagccgacc aggtggcgat gagtccattt catctacatc ggttgtttaa
    14821 agctactacc ggaatgacgc ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg
    14881 cgaatcgctg gcgaaagggg agagcgtgac gacgtctatt cttaacgccg gattccccga
    14941 cagcagcagt tactaccgca aagccgatga aacgctgggc atgacggcta aacaattccg
    15001 tcacggtggc gaaaatctgg cggtgcgtta cgcgctggct gattgtgagc tgggtcgttg
    15061 cctggtggca gaaagcgagc gggggatttg cgcgatattg ctgggcgatg atgacgcgac
    15121 attaatcagc gagttgcagc agatgtttcc cgctgccgac aacgcgcctg ccgatctgac
    15181 gtttcagcaa catgtgcgtg aagtgatcgc cagcctcaat caacgcgata cgccgctgac
    15241 gttaccgctg gatattcgcg gcactgcttt tcagcaacaa gtctggcagg cactgcgcac
    15301 gataccttgt ggtgaaaccg tcagttatca gcaactggct aacgccatcg gcaaaccgaa
    15361 agcggtacgg gccgttgcca gcgcctgtgc cgccaacaag ctggctatcg taataccctg
    15421 tcatcgagtg gtccgtggtg atggcacact ttccggttac cgctggggcg tgtcgcgtaa
    15481 agcgcaactg ctgcgccgcg aagctgaaaa tgaggagagg taatgttgga tctgtttgcc
    15541 gatgctgaac cgtggcaaga gccactggcg gctggtgcgg taattttacg gcgttttgct
    15601 tttaatgctg cggagcaact aatccgcgat attaatgacg ttgccagcca gtcgccgttt
    15661 cgccagatgg tcgctccagg cggatatacc atgtcggtgg cgatgaccaa ctgtgggcat
    15721 ctgggctgga cgacccatcg gcaaggttat ctctattcgc ccattgatcc gcaaacaaat
    15781 aaaccgtggc ccgccatgcc acaaagcttt catgatttat gtcaacgtgc ggctacggcg
    15841 gcgggctatc cagatttcca gccagatgct tgtcttatca accgctacgc tcctagcgcg
    15901 aaactgtcgc tgcatcagga taaagacgaa ccggatctgc gcgcgccaat tgtttctgtt
    15961 tctctgggct tacccgcgat ttttcaattt ggtggcctgg ggcggtgaat cgcggctgtt
    16021 ttatcacggt attcaaccgt tgaaagcggg gtttcatcca ctcaccaccg actgccgcta
    16081 caacctgaca ttccgtcagg caggtaaaaa agaataaaaa taagaattat tattgctgtg
    16141 cgcgcgaaga tgtttaaact gcgggctgta attcattgtc cgggttttct gcatggaact
    16201 tcttgtactt gtctggcggc agtatcgctg gccatttatc agtgtgatgg cgctaagcct
    16261 cgccagtgcg gcattaggca ttggcttaat tgcttttatc aatcagcgcc ttatcgaaac
    16321 ggcggatacc agtctgctgg tgttaccgga gtttctggga ttattgctgc tgttgatggc
    16381 agtcactctc ggatcgcaac tggcgctcac cactttgggg catcacttcg tttaccgact
    16441 gcgtagtgaa tttatcaagc ggattctgga tacccacgtc gagcgcattg aacaactcgg
    16501 tagcgcctcg ttgctggcgg gattaaccag cgatgtgcgc aatatcacca ttgcttttgt
    16561 gcgtctgccg gaactggggc aggggatcat tctcactatc ggttcatcgg cgtatctgtg
    16621 gatgctgtcg ggcaaaatgt tgctggtaac ggctatctgg atggcgatca ccatctgggg
    16681 cggttttgtg ctggtggcgc gggtgtacaa acatatggcg accctacgtg aaaccgaaga
    16741 caagctgtac acggattttc aaactgtact tgaagggcgc aaagagctga ctctgaaccg
    16801 ggaacgcgcc gagtatgtgt ttaacaacct ctacatcccc gatgcgcaag agtatcgcca
    16861 tcatattatt cgcgcagaca ccttccatct tagtgccgtg aactggtcaa acatcatgat
    16921 gctgggcgct atcggcctgg tgttctggat ggcgaacagt ctcggttggg ctgataccaa
    16981 cgttgctgcg acctattcgt tgacgctgtt attcctgcgt acgccgctgc tttcggcggt
    17041 tggcgcattg ccgacgctgc tgacggcgca ggtggcgttt aacaagctga acaaattcgc
    17101 gctcgcgcct ttcaaagctg agtttccgcg cccgcaggcg tttcccaact ggcaaacgct
    17161 ggagctgcgt aacgtgacgt ttgcttatca ggataacgcg ttttccgttg gtccgattaa
    17221 tctcaccatc aaacgtggcg agctgctgtt tctgattggc ggcaacggta gcggaaaatc
    17281 gacgctggcg atgttgttga cgggcttgta tcagccacaa agcggcgaaa tcttgctgga
    17341 tggcaaacct gtcagcggcg aacaaccgga agattatcgc aaactgtttt cggcagtgtt
    17401 taccgatgtc tggctgtttg atcaactgct ggggccggag gggcaacccg ctaacccgca
    17461 actggttgag aagtggctgg cgcagctgaa aatggctcat aagcttgagt taagcaacgg
    17521 gcgtattgtt aacctgaagt tatcaaaagg gcagaaaaaa cgcgtgccgc tgttgctggc
    17581 gctggcagaa gaacgcgata ttatcctgct ggatgaatgg gcggcggatc aggatccaca
    17641 cttccgtcgt gagttttatc aggtgttgct gccgctgatg caggagatgg gtaaaactat
    17701 tttcgctatc agtcatgatg atcattactt tatccacgcc gaccgcctgc tggaaatgcg
    17761 caatgggcaa cttagcgagc tgacgggcga agagcgcgat gccgcttcgc gtgatgccgt
    17821 tgcccggacg gcataatatt acgacgctga cctggcggac tgctgccgtc aggtcaatat
    17881 gctaaaaatc ccttcatttc taataaccct ataattaatc aacgaaatta taatgtttct
    17941 aaaattagaa tataatttat aaacattatt taaatgttgt tacttaagtg ttaccgttga
    18001 tgccgcgcaa atctcacctg caataaagcg actaaaagta aggcattaac aagatgaaaa
    18061 aagtgactgc catgctcttc tcgatggccg tggggcttaa tgccgtttcg atggcggcaa
    18121 aagcgaaagc gtccgaggag caggaaactg atgtactgtt gattggcggc ggcattatga
    18181 gcgccacgtt ggggacctat ttacgcgagc tggagcctga atggtcgatg accatggtgg
    18241 agcgcctgga ggggggcgcg caggagagtt cgaacggctg gaacaacgcc ggaaccgggc
    18301 attctgcact gatggaactg aactacaccc cgcaaaacgc cgatggcagc atcagtattg
    18361 aaaaagcagt cgccattaac gaagcatttc agatttcccg ccagttctgg gcgcaccagg
    18421 tcgagcgcgg cgtgctgcgt actccgcgtt catttatcaa taccgttccg catatgagct
    18481 ttgtctgggg cgaggataac gttaatttcc tgcgcgcccg ttacgccgcg ttgcaacaaa
    18541 gctcgctgtt tcgcggtatg cgttactctg aagatcacgc gcagatcaaa gagtgggcac
    18601 cgttagtgat ggaagggcgc gatccgcaac agaaattggc actgatgtga actatggtga
    18661 gatcacccgc cagttaattg cttccttaca gaagaaatct aacttctcgc tgcaactcag
    18721 cagcgaagtc cgcgccctaa agcgtaatga cgataacacc tggaccgtta ccgttgccga
    18781 tctgaaaaac ggcactgcac agaacatccg caccaaattt gtctttatcg gcgcgggcgg
    18841 tgcggcgctg aagctgttac aggaatcggg gattccggaa gcgaaagact acgccggttt
    18901 cccggtgggc ggacagttcc ttgtttcgga aaatccagac gtggttaatc gccatctggc
    18961 gaaagtttac ggtaaagcat ccgttggcgc accaccgatg tcggttccgc atatcgatac
    19021 ccgtgttctg gacggtaaac gcgtagtgct gtttgggcca tttgccacct tctcaaccaa
    19081 attcctcaaa aacggttcat tgtgggatct gatgagttcc accaccacct ctaacgtgat
    19141 gccgatgatg cacgtcgggc tggataattt cgatctggtg aaatatctgg tgagtcaggt
    19201 gatgttgagt gaagaggatc gttttgaagc gttgaaagag tactatccgc aagcgaaaaa
    19261 agaggactgg cgtttgtggc aagcggggca gcgtgtgcag attatcaagc gtgatgccga
    19321 gaaaggtggc gtactgcgtc tgggtaccga agtggtcagt gaccagcaag ggaccattgc
    19381 ggcgctgctg ggggcatcgc caggggcgtc aaccgccgcg ccgattatgt tgaatctgct
    19441 ggaaaaagta tttggcgatc gcgtttccag cccgcaatgg cagtctacgt tgaaagcgat
    19501 cgttccgtct tatggacgca agctgaacgg tgatgtagcg gcaacagaac gcgagttgca
    19561 gtacaccagc gaagtgctgg ggctgaaata cgacaaaccg caagcagcag atagtacgcc
    19621 gaaaccgcag ttgaaaccgc aacccgttca aaaagaagtg gcggatattg cgttgtaatg
    19681 atgtgccaca tccggcatgg tatgccggat gtggcgtata ctgataagac gcgccagcgt
    19741 cgcatcaggc aaccggctcg ggcgttagat gttggatgcg gcgtaaccgc cttatccaac
    19801 caacggggtg ccgtacttca caacagttta gcgaacttcc gcgttgtcaa ttttctcttc
    19861 cgccttccag acgcggtatt tcacatctac attgtctggc gtatacacca cgatcggcag
    19921 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
    19981 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
    20041 ataataatca tagccccagc cttccagcgt tttgctttcc agcttcccgc cgagacgatg
    20101 caaattgcaa tcgacttcca gcgtctgacc gattaacagt tctactttca gggtagattc
    20161 atcttcttgc ggggttaact gaatcacctg acgcttcatg cctgtttcag cttgtggata
    20221 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtagcgaa
    20281 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
    20341 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
    20401 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
    20461 atgagggagc aaacaagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
    20521 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
    20581 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
    20641 gcggctataa aaatggctta ttaattatgc ggtctatttg gttgctctca attttcagag
    20701 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
    20761 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
    20821 ttctgaccca ttgcacccgc tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
    20881 aacgcggcac gggtggctat ccgagcgtta atttcaaaaa taacgagtgc agcttctgtt
    20941 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggtctggg
    21001 atttgcagtt caccatcggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
    21061 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
    21121 aggcgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
    21181 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
    21241 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
    21301 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
    21361 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
    21421 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
    21481 gtcgtagctt tatgaaagct aacg
//
LOCUS       DADLTF010000064        21418 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.064, whole genome shotgun sequence.
ACCESSION   DADLTF010000064 DADLTF010000000
VERSION     DADLTF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.064"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..2005
                     /gene="speF"
                     /locus_tag="JN749_002212"
     CDS             <1..2005
                     /gene="speF"
                     /locus_tag="JN749_002212"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HAY7368614.1"
                     /translation="NEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASHYETQLRP
                     PFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLCNADVAMG
                     DLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVLFDRNNHK
                     SNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQRAKEARPF
                     RLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCSPLLLDLN
                     ENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFY
                     PLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQS
                     YETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPYKLLLTTPGIDARNGEYEAFGV
                     PATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLA
                     EVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHVSMNPQEA
                     NYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGCAVLRYFSALEEGIN
                     LLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="JN749_002213"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="JN749_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HAY7368615.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSITMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JN749_002214"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="JN749_002214"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HAY7368616.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JN749_002215"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="JN749_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HAY7368617.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="JN749_002216"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="JN749_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7368618.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6608..6970
                     /gene="ybfE"
                     /locus_tag="JN749_002217"
     CDS             6608..6970
                     /gene="ybfE"
                     /locus_tag="JN749_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HAY7368619.1"
                     /translation="MYYGALSIRAEAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="JN749_002218"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="JN749_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HAY7368620.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="JN749_002219"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="JN749_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HAY7368621.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="JN749_002220"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="JN749_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HAY7368622.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /locus_tag="JN749_002221"
     CDS             complement(8459..8785)
                     /locus_tag="JN749_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368623.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JN749_002222"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="JN749_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HAY7368624.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLFHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="JN749_002223"
     CDS             complement(10807..12471)
                     /gene="glnS"
                     /locus_tag="JN749_002223"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HAY7368625.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="JN749_002224"
     CDS             complement(12674..14620)
                     /gene="nagE"
                     /locus_tag="JN749_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HAY7368626.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14953..15753
                     /gene="nagB"
                     /locus_tag="JN749_002225"
     CDS             14953..15753
                     /gene="nagB"
                     /locus_tag="JN749_002225"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7368627.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15813..16961
                     /gene="nagA"
                     /locus_tag="JN749_002226"
     CDS             15813..16961
                     /gene="nagA"
                     /locus_tag="JN749_002226"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7368628.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16970..18190
                     /gene="nagC"
                     /locus_tag="JN749_002227"
                     /gene_synonym="nagR"
     CDS             16970..18190
                     /gene="nagC"
                     /locus_tag="JN749_002227"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HAY7368629.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18238..18980
                     /gene="nagD"
                     /locus_tag="JN749_002228"
                     /pseudo
     CDS             18238..18980
                     /gene="nagD"
                     /locus_tag="JN749_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19377..21041
                     /gene="asnB"
                     /locus_tag="JN749_002229"
     CDS             19377..21041
                     /gene="asnB"
                     /locus_tag="JN749_002229"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HAY7368630.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
BASE COUNT         5265 a         5428 c         5403 g         5322 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatc agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggttg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggcg cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatca ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcctg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cctgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgagtcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga gctgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctggcagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgaagtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgaaaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaaataaaa attaaaaaag tagcgttgag aaaagtctcg
    10321 ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt ctgtaaccag
    10381 aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag gcttaagttt
    10441 gcggttgaaa ggcttgcaga agaataaaaa cgtgaaattc cagcaagatg aaagcggtta
    10501 cattttattt aattatgttt tgtgatttta aatttatttt atggataaat tgcgaagcgg
    10561 aattaattcg cgaagaaaag cagaaaaaac gccgcagagc gcggcgtttg ggattgtttg
    10621 ctatttcttg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaagca cgcaaattca
    10681 atatattgca gagatcatgt aggcctaata agcgaagcgc atcaggcaat ttagcgtttg
    10741 aaatttattg tctatttatt gccggatgcg acgtaaatgc ctcacccggc ttggtagaac
    10801 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
    10861 cggtttttcg gccgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg
    10921 gaatgcttta cccgctaccg catctttcag cgacggttca gcaaagccct gtttgatcac
    10981 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcacctgggt taggcacgct
    11041 gaacagacga tcatacaaac ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg
    11101 aataacacct ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata
    11161 agtacagaag atggtggtga tattaccttc ggcatctttc tccacgcgtt cagccttaat
    11221 gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
    11281 agcttcttcg cggaaatcgg cgcgatcgat ccaaatttca ccgctaaacg gcacctgacg
    11341 gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg
    11401 atagttttcg ataaccagtt tcaccggatc gataactgcc attgcgcgcg gcgcattttc
    11461 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
    11521 ggtcacgccg atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg
    11581 cagaccggaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
    11641 cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc
    11701 gtactggcgc ggatgaacag gaatggtgat gttatccagt acccagtcgt acagacgacg
    11761 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat
    11821 gcaatgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
    11881 agcaaattta atgcggtaca gcaccggatc gcgcattacg ataaatggcg acgccatgtc
    11941 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
    12001 cagcgccaag ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggctgcgt
    12061 cagggtaccg cggtattcgc ggatctgttc cggtgtcagt tcatcaacgt acgccaggcc
    12121 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
    12181 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg aatcgacata
    12241 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
    12301 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
    12361 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
    12421 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
    12481 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
    12541 gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa
    12601 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctgccg tgtcgcatca
    12661 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
    12721 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
    12781 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
    12841 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacgg
    12901 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
    12961 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
    13021 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
    13081 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
    13141 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
    13201 cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
    13261 tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca
    13321 gacgtttaca catcgtatcg ttgacgcggg cagagtcagc cacggtcagg cgcagacggg
    13381 taatacaggc atcaatcgct ttcaggttgt cagtaccgcc aaccgcagca atatagttgg
    13441 ttgccagttg agtcagacct tcttcagtat tgctgttggc ttcttcagta acgatctcgt
    13501 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
    13561 ccacgaagta gatagcgaag aagataacgc ccatcaccag cagcatccag acgttctggc
    13621 tggcggccgg caggttatac atcaacgcgt agtcgatagc acccgcagag aaagagaagc
    13681 cggcatggat acccagcagc gttgccacaa acaggctgat accggtcagc agtgcgtgca
    13741 ggaggtacag cagcggcgca aggaacatga acaggaattc cagcggctca gtcacaccgg
    13801 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
    13861 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc atgatcggga
    13921 agaagccgga catgaacatc cccgcggtgc cgtcaccagc gtagaaacgg ttgatgtcgc
    13981 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
    14041 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
    14101 aacccagcgc gcccgcagaa acgatccact cgccgcctgc atggatagcg tgctgtaccg
    14161 gcggccagac gtaaccaaaa atggccgcca gcaccagaca gaagaagccg gtggcgatcg
    14221 gcacaaagcg tttgccgccg aagaagctca ggaagtccgg cagtttaata tcggaccaac
    14281 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
    14341 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
    14401 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
    14461 atgcgaggtt atcaaaaatt gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
    14521 ctggctgacc gaatcgcagc aacagcgccg ccaccggcag caccgcgata gggagctgta
    14581 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
    14641 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattttact gccgatgttc
    14701 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
    14761 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
    14821 ctggctaaac cagaaaactt attttatcat tcaaaaaatc aagtcggatt gacgcctgtc
    14881 tgcgcaaatc caggttacgc ttaaagatgt ctaatccgcc aacggcttac attttactta
    14941 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
    15001 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccaa ctgccgatcg tccgtttgta
    15061 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
    15121 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
    15181 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
    15241 gttgatattc cagcagaaaa catcaacctt ctcaacggca atgccccgga tatcgatgcc
    15301 gagtgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
    15361 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
    15421 actcgtatca aaaccctgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
    15481 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacact gctggatgcc
    15541 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcgctgca ggccgccgtt
    15601 gaaggttgcg tgaaccatat gtggaccatc agctgtctgc aactgcatcc gaaagcgatc
    15661 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
    15721 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gttccttgct
    15781 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
    15841 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
    15901 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
    15961 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
    16021 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
    16081 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
    16141 gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc
    16201 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
    16261 agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga
    16321 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
    16381 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg
    16441 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
    16501 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatc gctgatggcc
    16561 tgcatgttga ttacgccaac attcgtaacg ctaaacgcct taaaggcgac aaactgtgtc
    16621 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
    16681 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
    16741 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
    16801 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tgacgttgaa aaacgtctcg
    16861 gcacgctcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca
    16921 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
    16981 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
    17041 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
    17101 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
    17161 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
    17221 caatttccac gcaatcggcg tacggcttgg tcgtcatgac gccaccatca ctctgtttga
    17281 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcagac
    17341 gctggaacat gccctgctga atgccattgc tcagtttatt gatagctacc agcgcaagct
    17401 gcgcgagcta atcgccattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
    17461 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag cactgaaaga
    17521 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
    17581 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
    17641 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggta acgtcggtga
    17701 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
    17761 cctggaaact atcgccgcca acgctgccat tgaacaacgg gtgttgaatc tgttaaagca
    17821 gggctaccag agccgtgtgc cactggacga ctgcaccatc aaaactatct gcaaagccgc
    17881 gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggtaa
    17941 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ctggtgaaat
    18001 caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaagcgct
    18061 gaaggcgttt cgtactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
    18121 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
    18181 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
    18241 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
    18301 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
    18361 accaactatc cttcgcagac tgggcaagat ctggcgaacc gctttgccac tgccggtgtc
    18421 gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ccgccgattt cctgcgtcgc
    18481 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
    18541 gccggtttca ctattaccga tgtgaaccct gatttcgtga ttgttggcga aacacgttcc
    18601 tacaactggg acatgatgca taaagcagcc tatttcgtcg ctaacggtgc acgctttatc
    18661 gccaccaacc cggacacggt ttttatcccg cttgtggcgc gttgtgtgca gggattgaga
    18721 aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc atccgcgcgg
    18781 cattaaacaa aatgcaggcg cattcggaag aaacagtgat tgtcggcgat aacctgcgta
    18841 ccgatattct ggcgggcttc caggccggtc tggagacgat tctggtgctt tctggtgttt
    18901 catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac ccgtcagtcg
    18961 ctgaaatcga cgttatctga ataccaccca cgtctgcgga cgtggttggc tctgatgaca
    19021 aacgccaaac tgcctgatgc gctacgctta tcaggcctac gcagctcctg caatatattg
    19081 aattttcatg cttttgtagg ccgtataagg cgttcacgcc gcatccggca tgaacaaatc
    19141 gcactttatc agcaatttgg cccccgtaaa atataaccat tcaataaaac ccgctaaaaa
    19201 ttatcgattc atcaataaat acatcctcac accagaccct ttttcaccaa tcgacaatca
    19261 ccattacgtt ttttattttt gcactgccaa atagcttgcg cacagtgctg ctttgcggca
    19321 tttttttaaa caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt
    19381 gttcaatttt tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac
    19441 tcgagctgtc acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg
    19501 ataacgccat tctcgctcac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac
    19561 ctctctacaa ccaacaaaaa acccacgtac tggcggtaaa cggtgaaatc tacaaccacc
    19621 aggcattgcg cgctgaatat ggcgatcgtt atcagttcca gacagggtct gactgtgaag
    19681 tgatcctcgc gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt
    19741 ttgcctttgc cctgtacgac agcgaaaaag atgcctacct gattggtcgc gaccatctgg
    19801 ggatcatccc actgtatatg gggtatgacg aacacggtca gctgtatgtg gcctcagaaa
    19861 tgaaatcgct ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt
    19921 ggagtcagga cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag
    19981 tgaaagataa cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa
    20041 gccatctgat gtctgatgtg ccttacggtg tgctactttc tggtggtctg gattcctcaa
    20101 ttatttccgc tatcaccaag aaatacgcag cccgtcgcgt ggaagatcag gaacgctctg
    20161 aagcctggtg gccgcagtta cactcctttg ctgtaggtct gccgggttca ccggatctta
    20221 aagcagccca ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg
    20281 tacaggaagg tctggatgcc atccgcgacg tgatttacca catcgaaact tatgatgtga
    20341 ccactattcg cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcgatgggca
    20401 ttaaaatggt gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc
    20461 ataaagcgcc caacgctaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc
    20521 atatgtatga ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg
    20581 ttccgttcct cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa
    20641 tgtgcggtaa cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc
    20701 ccgcaagcgt ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga
    20761 tcgacaccct gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc
    20821 gcttccgctt cccgtacaac acgccgacct caaaagaagc gtatctgtac cgcgagatct
    20881 ttgaagaact gttcccactt ccgagtgccg ctgagtgcgt gccgggcggt ccttccgtcg
    20941 cgtgttcttc agctaaagcg atcgaatggg atgaagcgtt caaaaaaatg gacgatccgt
    21001 ctggtcgtgc ggttggcgtt caccagtcgg cgtataagta agaaatgcgg tactgccccg
    21061 gagaaatccg gggctgtttt agttattgcg ctgcttgttg attctgatat gcagtaagga
    21121 aatgttccgg gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat
    21181 agattaattt cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg
    21241 gctgtagggt ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg
    21301 ttcgcaacct aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct
    21361 gcaaggctct atacgcataa tgcgccccgc aacgcagata aggtatcgcg aaaaaaag
//
LOCUS       DADLTF010000065        21401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.065, whole genome shotgun sequence.
ACCESSION   DADLTF010000065 DADLTF010000000
VERSION     DADLTF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.065"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="JN749_002230"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="JN749_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAY7368631.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="JN749_002231"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="JN749_002231"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HAY7368632.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JN749_002232"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="JN749_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HAY7368633.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTQLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JN749_002233"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="JN749_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HAY7368634.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JN749_002234"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="JN749_002234"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAY7368635.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6616..7110
                     /gene="lrp"
                     /locus_tag="JN749_002235"
     CDS             6616..7110
                     /gene="lrp"
                     /locus_tag="JN749_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HAY7368636.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7245..11351
                     /gene="ftsK"
                     /locus_tag="JN749_002236"
     CDS             7245..11351
                     /gene="ftsK"
                     /locus_tag="JN749_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAY7368637.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11506..12117
                     /gene="lolA"
                     /locus_tag="JN749_002237"
     CDS             11506..12117
                     /gene="lolA"
                     /locus_tag="JN749_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HAY7368638.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12128..13471
                     /locus_tag="JN749_002238"
     CDS             12128..13471
                     /locus_tag="JN749_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAY7368639.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13562..14854
                     /gene="serS"
                     /locus_tag="JN749_002239"
     CDS             13562..14854
                     /gene="serS"
                     /locus_tag="JN749_002239"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAY7368640.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15094..17538
                     /gene="dmsA"
                     /locus_tag="JN749_002240"
                     /pseudo
     CDS             15094..17538
                     /gene="dmsA"
                     /locus_tag="JN749_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            17549..18166
                     /gene="dmsB"
                     /locus_tag="JN749_002241"
     CDS             17549..18166
                     /gene="dmsB"
                     /locus_tag="JN749_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HAY7368641.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     HYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18168..19031
                     /locus_tag="JN749_002242"
     CDS             18168..19031
                     /locus_tag="JN749_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase"
                     /protein_id="HAY7368642.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19067..19693)
                     /locus_tag="JN749_002243"
     CDS             complement(19067..19693)
                     /locus_tag="JN749_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7368643.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20008..21156
                     /locus_tag="JN749_002244"
     CDS             20008..21156
                     /locus_tag="JN749_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368644.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         5282 a         5376 c         5657 g         5086 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaact tgtgcagtat
      121 atctacatcg agacaagtta tggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaattcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag caccgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tgaccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agttctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tttgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag atctccgaca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct cgccctgttg
     2041 cgggtcccat gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgct atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacctc cggtttttga tccgttaagt cagagatacg
     2281 tacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcac aaaagacaaa cagggcaatt aatgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcaccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata acgctgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcaggtaaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcggttgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcaacaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgcg gcaccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc tgcgtcacaa ttgccagcac aataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat tttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cacgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcggccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc agcggatcgg tcaatgctaa
     3961 ttccgcctca ccgcgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gtaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacctgca gccagcgtca ccccagtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gtcgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca ttccaaccag
     4441 cgccataaac aacggaatta gcggtgctgt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 cagatagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca gcgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca atacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gagatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcaaggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagccagtac cggccgaagt aatggcctgg cgataaatgt gatccatcac gtcgcctgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgtactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatcg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccggc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaatc agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg cgccattaat
     5881 aacggaccgg tcagatcgtt tggatcacca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 ttgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 acagtattct tatatgcgca taaccatgta tgtaaatacc atgtttaccg tgctagtgaa
     6421 atctacgtat ggcgtggaca gacgccattc gtgatgtcga tagctgccac aaggcaacgg
     6481 tcttctcacc gtagacccag gcattgcgcg ccgtgaatct tcatgatttc ggtctatcgt
     6541 gacgggtagc gactctgaac agtgatgttt cagggtcaga caggagtagg gaaggaatac
     6601 agagagacaa taataatggt agatagcaag aagcgccctg gcaaagatct cgaccgtatc
     6661 gatcgtaaca ttcttaatga gttgcaaaag gatgggcgta tttctaacgt cgagctttct
     6721 aaacgtgtgg gactttcccc aacgccgtgc cttgagcgtg tgcgtcggct ggaaagacaa
     6781 gggtttattc agggctatac ggcgctgctt aacccccatt atctggatgc atcacttctg
     6841 gtattcgttg agattactct gaatcgtggc gcaccggatg tgtttgaaca attcaatacc
     6901 gctgtacaaa aacttgaaga aattcaggag tgtcatttag tatctggtga tttcgactac
     6961 ctgttgaaaa cacgcgtgcc ggatatgtca gcttaccgta agttgctggg ggaaaccctg
     7021 ctgcgtctgc ctggcgtcaa tgacacacgg acatacgttg ttatggaaga agtcaagcag
     7081 agtaatcgtc tggttattaa gacgcgctaa cacggaacag gtgcaaaatc ggcgtatttt
     7141 gattacactc ctgttaatcc atacagcaac agtactgggg taacctggta ctgttgtccg
     7201 ttttagcatc gggcaggaaa agcctgtaac ctggagagcc tttcttgagc caggaataca
     7261 ctgaagacaa agaagtcaca ttgacaaagt taagtagcgg ccgccgcctt ctggaggcgt
     7321 tgctgatcct tattgtcctg tttgccgtct ggttgatggc tgccttacta agctttaacc
     7381 cttcggaccc cagctggtcg caaacggcct ggcatgaacc tatccataat ttaggtggga
     7441 tgcccggtgc gtggttggca gatacgctgt tctttatttt tggcgtgatg gcttacacca
     7501 ttcccgtcat tattgtcggc ggttgttggt ttgcctggcg tcatcagtcc agcgacgaat
     7561 acattgatta ttttgccgtt tcgctacgca tcattggcgt tttggcgctc atccttacct
     7621 cctgtggtct ggcggcaatc aacgctgacg atatctggta ttttgcctcc ggtggcgtca
     7681 ttggcagctt actaagcact acgctacaac cactgctaca cagtagcggg ggaactattg
     7741 cgctgctctg cgtttgggca gcgggcctga cgttgttcac cggttggtca tgggtgacca
     7801 ttgctgaaaa actcggcggc tggattttaa acattctcac cttcgccagt aatcgtaccc
     7861 gtcgcgatga tacctgggtc gatgaagatg agtatgaaga cgacgaagag tatgaagatg
     7921 aaaatcacgg caaacagcat gaatcacgcc gtgcccgtat tcttcgcggc gcgctagcgc
     7981 gtcgtaaacg gttggcggaa aaattcatta atccgatggg gcggcaaaca gacgctgcgt
     8041 tgttctccgg taagcggatg gatgatgacg aagagattac ctacactgca cgcggtgtgg
     8101 ctgccgaccc ggacgacgtc ctatttttgg gcaatcgtgc aacgcagcca gaatatgacg
     8161 aatacgatcc attattaaac ggtgcgccaa ttaccgaacc tgtcgctgta gcagctgctg
     8221 ctaccacggc gacacaaagc tgggctgcgc cggttgaacc tgtgactcag acgccgcctg
     8281 ttgcctctgt tgatgttcca ccttcgcaac ctacagtagc ctggcagcct gtaccgggtc
     8341 cacaaacggg agagccggtt attgctcctg caccggaagg ttacccacag cagtcacaat
     8401 atgcgcagcc tgcagtgcaa tataatgagc cgctgcaaca accagtacag ccgcagcagc
     8461 cgtattatgc acctgcagct gaacaacctg cgcaacaacc ttactacgct cctgcggcgg
     8521 aacaacctgt tcagcaaccg tattacgcaa ctgcgccgga acagcctgcg caacagccgt
     8581 attatgcccc tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc
     8641 aatccatttt tgctccacag tctacatacc agactgagca aacttatcag cagccagccg
     8701 ctcaggagcc gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg
     8761 aacccgttgt agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg
     8821 aagagaagcg agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac
     8881 cggttaaaga accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac
     8941 ctccagtaga agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac
     9001 tggcgacggg ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg
     9061 gaccgcgtcc gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc
     9121 gcgtgccaac tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca cagcgtgcgg
     9181 cggaagaaaa agcccgtgaa gcccggcgca atcagtatga ttctggcgat cagtataacg
     9241 atgatgaaat cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc
     9301 agcaacgcta tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg
     9361 ctgcggcaga ggctgaactg gctcgtcagt ttacgcaaac tcaacaacaa cgttattccg
     9421 gcgaacaacc ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga
     9481 aagcgttgct ggatgatggt ccacacgaac cgctgtttac gccaattgtt gaacctgtac
     9541 agcagccgca acaaccggtt gcaccacagc agcagtatca gcagccacaa cagccggttg
     9601 cgccacagcc gcagtatcag cagccacaac agcaggttgc gccgcagcca caatatcagc
     9661 agccgcaaca accggttgcg ccacagcagc aatatcagca gccgcaacaa ccagttgcgc
     9721 cgcagccgca gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc
     9781 cacaacagcc agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta
     9841 atggcgacag ccgtccgttg cacaaaccga cgacgccgct gccttctctg gatttgctga
     9901 caccgccgcc gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggcgcgtc
     9961 tggtggaagc gcgtctggct gatttccgta ttaaagccga tgtcgttaat tactctccgg
    10021 ggccagttat cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt
    10081 ctaacttgtc acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag
    10141 ttattcctgg caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt
    10201 atctgcgcga agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg
    10261 tgctgggtaa agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact
    10321 tgttggttgc ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga
    10381 gcatgcttta taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc
    10441 tggagctttc ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga
    10501 aagatgccgc caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga
    10561 tgtctgcgct gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc
    10621 gcatgatgcg tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc
    10681 atccggtgct gaaaaaagaa ccatacattg tcgtgttggt tgacgaattt gccgacctga
    10741 tgatgacggt aggtaaaaaa gtggaagagc tgatagcacg tctggcgcaa aaagcccgtg
    10801 ccgcgggtat ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc
    10861 tgattaaagc gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac
    10921 gtaccattct tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact
    10981 ctgggccgaa ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag
    11041 ttcatgccgt ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca
    11101 cctccgacag cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc
    11161 cgttgttcga tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg
    11221 tacagcgtca gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag
    11281 cgcaggggat tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc
    11341 cgtttgacta attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat
    11401 caactgaaaa ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag
    11461 tagagggaac tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgct
    11521 atcacctgtg cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc
    11581 gatctgaaaa gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg
    11641 actgacggta gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca
    11701 aacttattca actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa
    11761 acactgtggt tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc
    11821 accggtaata cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac
    11881 aatatcaaac agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg
    11941 aagcagttca ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag
    12001 caggacgatc agcgcagcag ttatcagctg aaatcccagc aaaatggggc tgtggatgca
    12061 gcgaaattta ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg
    12121 cacctgagtg agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc
    12181 gcgtatgcgg ccagaaaatt tagcacagta tatcggtcag caacatttgc tggctgcggg
    12241 gaagccgttg ccgcgcgcta tcgaagccgg gcatttgcat tctatgatcc tctgggggcc
    12301 accgggtacc ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt
    12361 ggaacgtatt tctgccgtca cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc
    12421 ccggcaaaac cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt
    12481 caacaaaagc cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat
    12541 tggcgcaacc actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg
    12601 tgtttatctg ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat
    12661 ggaagacaaa acccgtggct atggtggtca ggatattgtt ctgccagatg atacacgacg
    12721 tgccattgca gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat
    12781 ggcggatatg gccgaagtct atgatagcgg taagcgggtc ctgaagcctg aattactgac
    12841 cgaaatcgcc ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct
    12901 aatttccgca ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta
    12961 tgcgcgaatt attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat
    13021 tgcgtctgaa gacgtcggta atgccgatcc acgggcgatg caggtggcaa ttgcggcctg
    13081 ggattgcttt actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt
    13141 ttacctggcc tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc
    13201 cgatgctcgc gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa
    13261 attaatgaag gaaatgggct acgggcagga atatcgttac gctcatgatg aagcaaacgc
    13321 ttatgctgcc ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc
    13381 gacaaacagg ggccttgaag gcaagattgg cgaaaagctc gcctggctgg ctgaacagga
    13441 tcaaaatagc cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt
    13501 atccctgtgg tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag
    13561 catgctcgat cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg
    13621 ccggggcttt aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt
    13681 gcaggtcaaa acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca
    13741 ggcgaaagcg cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga
    13801 agagctggat gcagcaaaag ccgagctaga tgctttacag gctgaaattc gcgatatcgc
    13861 gctgaccatc cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa
    13921 cgttgaagtc agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt
    13981 gacgctgggt gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc
    14041 ccgctttgtg gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat
    14101 gctggatctg cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa
    14161 ccaggacacg ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac
    14221 tcgtccgctg gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt
    14281 tccgctgact aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat
    14341 gaccgcccac accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg
    14401 tctgatccgt atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccagaaga
    14461 ctcaatggcg gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg
    14521 cctgccgtac cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac
    14581 ttacgacctg gaagtatgga tcccggcaca gaacacctac cgtgagatct cttcctgctc
    14641 caacgtttgg gatttccagg cacgtcgtat gcaggcacgt tgccgcagca agtcggacaa
    14701 gaaaacccgt ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt
    14761 tgcagtaatg gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg
    14821 tccgtatatg aacggactgg aatatattgg ctaataccca atttttctga atctaaaaag
    14881 cgcctttagg gcgctttttt tgtctccctt tgataccgaa caataattac tcctcactta
    14941 cacgtaatac tactttcgag tgaaaatcta cctatctctt tgattttcaa attattcgat
    15001 gtatacaagc ctatatagcg agctgctata gaaataatta cacaatacgg tttgttactg
    15061 gaatcaatcg tgatcaagct tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta
    15121 ttggctgctg aggagagtcg ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca
    15181 atggccagca gcgcattaac attacctttt agtcggattg cgcacgctgt cgatagcgcc
    15241 attccaacaa aatcagacga aaaggttatc tggagcgcct gtacagttaa ctgtggtagt
    15301 cgctgcccgc tacgtatgca cgtcgtggac ggtgaaatca aatatgtcga aacggacaat
    15361 accggcgatg acaattacga cggcctgcac taggttcgcg cctgcctgcg tgggcgttcc
    15421 atgcgtcgcc gtgtctacaa tccggaccgc ctgaaatatc cgatgaaacg agtcggggcg
    15481 cgcggtgaag gcaaattcga gcgcattagc tgggaagaag cctacgacat catcgcgacc
    15541 aatatgcagc gcctgatcaa agagtacggc aacgagtcta tctatctgaa ctatggcacc
    15601 ggtacgctgg gcggcaccat gacccgctcc tggccgccgg gaaataccct ggtcgcgcgc
    15661 ctgatgaact gctgcggcgg ttatcttaac cattacggtg actactcttc cgcgcaaatt
    15721 gcggaaggtt tgaaccatac ctacggcggc tgggcagatg gtaacagccc gtcggatatc
    15781 gaaaacagta aattggtagt gctgtttggt aataacccag gcgaaacgcg aatgagtggc
    15841 ggtggggtga cttactatct tgaacaggca cgccagaaat ctaatgctcg cataatcatc
    15901 atcgatccgc gctataccga caccggtgcc gggcgcgaag atgagtggat ccctattcgt
    15961 ccaggaacag atgccgcact ggttaacggt ctggcgtacg tcatgatcac tgaaaacctg
    16021 gtggatcagg cattcctcga taaatattgc gttggctatg acgagaaaac cctgcccgcc
    16081 agtgcgccga aaaatggtca ttataaagct tatattctgg gtgaagggcc agatggcgtg
    16141 gctaaaacac cggaataggc ctcgcaaatc accggtgttc cggcagacaa aatcatcaaa
    16201 ctggctcgtg aaatcggcag taccaaaccg gcgtttatca gccagggatg gggcccgcag
    16261 cgtcacgcta acggtgaaat cgcaacccgt gctatctcga tgctggcgat tctgaccggt
    16321 aacgttggta ttaatggagg gaacagcggc gcgcgtgaag gttcatacag cttaccgttt
    16381 gtccgtatgc cgaccttgga aaacccgatc cagaccagca tttcgatgtt tatgtggacc
    16441 gatgccattg aacgtggccc ggaaatgacg gcgctgcgtg atggtgtgcg cgggaaagat
    16501 aagctggatg tgccgatcaa aatgatctgg aactatgccg gtaactgcct gattaaccag
    16561 cattctgaaa tcaaccgtac ccatgaaatc ctccaggatg ataagaagtg cgagctgatt
    16621 gtggttatcg actgccacat gacctcatcg gcgaaatatg ctgacatcct gctgcctgac
    16681 tgcaccgctt ccgaacagat ggactttgca ctggatgcat cctgcgggaa tatgtcttac
    16741 gtgattttca acgatcaggt gattaaaccg cgctttgaat gtaagaccat ctatgaaatg
    16801 accagcgaac tggcaaaacg tcttggcgtt gagcaacagt ttactgaagg ccgtacccag
    16861 gaagagtgga tgcggcatct gtatgcccag tcgcgggaag cgattcctga actgccaacg
    16921 tttgaagagt tccgcaagca ggggatcttt aaaaagcgcg acccacaagg gcatcacgtt
    16981 gcttataaag ccttccgtga agatccgcag gcaaacccac tgactacgcc atcgggcaaa
    17041 attgagattt attcgcaggc gctggctgac attgccgcta cctgggaatt gccagaaggc
    17101 gatgtgatcg atccactgcc gatctacacg ccgggctttg aaagttatca ggatccgctg
    17161 aacaaacagt atccgctgca gcttacaggt ttccactata aatctcgcgt tcactcaact
    17221 tacggcaacg ttgatgtgct gaaagcggct tgccgtcagg aaatgtggat caacccgctt
    17281 gatgcccaaa aacgcggtat ccacaacggc gataaagtca ggatctttaa cgatcgtggt
    17341 gaggttcata ttgaggcgaa agtgacgcca cgaatgatgc cgggcgtggt cgcactgggt
    17401 gaaggcgcct ggtatgaccc ggatgcaaaa cgtgtcgata agggcggttg tatcaacgta
    17461 ctgaccactc aacgtccgtc tcctctcgct aaggggaatc cgtcacatac aaaccttgtt
    17521 caggttgaaa aggtgtaagg agtaaccgat gacaacccag tatggatttt ttattgattc
    17581 cagccgttgc accggttgca aaacctgcga gctggcttgt aaagactaca aagatttgac
    17641 gccagaagtc agtttccgcc gcatttatga atatgctggc ggcgactggc aggaagataa
    17701 cggtgtctgg caccagaacg tgtttgccta ctatctgtcg atttcgtgca accactgcga
    17761 agatccggct tgtactaaag tctgcccgag cggtgcgatg cataaacgcg aagatggttt
    17821 tgttgtggtc gatgaagatg tatgcattgg ctgccattac tgtcatatgg cttgcccgta
    17881 tggcgcaccg caatataacg aaacgaaagg ccatatgacc aaatgcgatg gttgttatga
    17941 ccgtgttgcg gagggtaaaa agccgatctg tgttgaatct tgcccgctgc gggcgctgga
    18001 tttcggccct atcgacgagc tgcgtaaaaa acatggcgat ctggcggccg ttgcgccgtt
    18061 gccgcgagct cactttacca aaccgaatat tgtgatcaaa cccaatgcca atagccgccc
    18121 gacaggggat accactggct atctggcaaa cccgaaggag gtgtgagatg ggaagtggat
    18181 ggcatgaatg gccgctgatg atcttcacgg tcttcgggca atgtgtggcg ggtggtttta
    18241 tcgttctggc tttggcgctg ctcaaaggcg acctgcgagc agaagcccag cagcgtgtta
    18301 tcgcctgcat gtttggctta tgggtactga tgggcattgg ctttatcgcc tctatgctcc
    18361 atcttggttc accgatgcgc gcctttaact cgctcaaccg ggtaggggct tcagcactca
    18421 gtaacgaaat cgccagcggt tcgatctttt ttgctgtagg cggcatcggc tggttgctgg
    18481 caatgctgaa aaagctgtca ccggcattgc gtacgctatg gctggtggtg acgatggttc
    18541 ttggcgtcgt ctttgtctgg atgatggtgc gtgtgtataa cagcattgat accgttccga
    18601 cctggtacag catctggacg ccgatgggct tcttcctgac gatgttcatg ggcggcccgt
    18661 tactgggtta cctgttgctg agtctggctg gcgtcgatgg ctgggcgatg cgtctgctgc
    18721 cagccatttc tgtactggca ctggtggtga gtggcgtgat gtcagtgatg cagggcacag
    18781 agctggcaac tattcatagt tctgtgcagc aggccgcagc gctggtgccg gactatggtg
    18841 cgctgatgtc ctggcggatc gtgcttttgg ccgttgccct gtgcttgtgg attgcaccac
    18901 agctaaaagg ttatcagcct gcggttccgc tactatccgt ctcattcatt ctgcttctgg
    18961 caggggaatt aatcggtcgc ggcgtattct atggtttgca tatgaccgtg gggatggccg
    19021 tcgcaagcta atcagtaaca accggggttt cggccccggt tctcttttat ttctgcttcg
    19081 ttaacgtgtc ataactggtc atcaaattac gataatcagg aatatggtta gagaacaacg
    19141 tcgccagtcc ttcaatatca ttacgccagt cacggtgcag ctcgcaagcc acgccgaacc
    19201 atgtcatcaa ttgcgcacca gcttgcgaca tgcgatccca cgccgaatgc cgggtaattt
    19261 cattaaaggt ccctgatgca tcagtcacga caaagacatc aaacccttct tcaatggctg
    19321 aaagcgccgg gaatgccacg caaacttcgg ttaccacacc ggcaataatt aactgttttt
    19381 tacctgtcgc tttgactgct tttacaaaat cttcgttatc ccaggcgtta atattgcccg
    19441 ggcgagcaat gtaaggtgca tcggggaatt gtgctttcag ttctagaact aatgggccgt
    19501 taggaccggt ttcaaaactg gtggtaagaa tagttggtaa gttgaaatac ttagccaggt
    19561 cacccagcgc cagcacgtta tttttaaact tatcgggttc gatatcccgt acaagggaaa
    19621 gtaaaccagc ctggtgatca acaagcaaaa cggcagcatc atttttatca agacgaacat
    19681 acggtttggt catcctcttc tcctttcgag agtcagtggt acgtctgaaa tcggtcagac
    19741 gcgatgcatt gctctgaaag catagacggg aaatatgagt ttgctgtgac catgaaattt
    19801 ttcgactgaa cgaagcgtat gaaatttgtg ttagatcaat aaaaacaatc agatgagatt
    19861 aatcatctct tcagcattag cgttatgaat gagtaatata aatcgataat accgccgctg
    19921 cgatatgcgc gtattttcac ccattgacaa tgtttttggc ggtggcatga tgcgcatgaa
    19981 atttgaactt cctcacggtt ttaattcatg tccacgtata cccggcctgt catgctttta
    20041 ctgtctggcc tgcttttgtt gactctggcg atcgcggtgt taaatacact cgtgccgctt
    20101 tggctcgccc aggaacacat gtccacatgg caggtaggcg ttgtcagctc atcctatttt
    20161 accggcaacc ttgtcggtac attgctgaca gggtatgtca ttaaacgcat tggctttaac
    20221 cgcagctatt atctgtcctc cttcattttt gctgctggct gtgccggcct tggcctgatg
    20281 attggtttct ggagctggtt ggcgtggcgt tttgtcgcgg gcgtcggctg tgccatgatt
    20341 tgggtggttg ttgagagcgc gctgatgtgc agtgggacgt cacgtaaccg tgggcgtttg
    20401 cttgctgcgt atatgatggt ttattacgtg ggaacgtttt taggccagtt actggtcagc
    20461 aaagtctcaa ccgagctgat gagcgtattg ccgtgggtta caggtttgac attggcaggg
    20521 atcttaccgc tgttgtttac gcgtgtcctg aatcagcagg ctgaaaacca tgattcgacg
    20581 tcaattacgg caatgctaaa actccgtcag gcgcggcttg gcgtgaatgg ctgcattatc
    20641 tcaggaatcg ttctgggatc tctatatggc ctgatgcctc tgtacctcaa tcacaaaggg
    20701 gtgagcaatg ccagtattgg tttctggatg gcggtactgg tcagtgcggg tatcctcgga
    20761 caatggccga ttggacgtct ggcggataag tttggtcgac tgctggtttt gcgtgttcag
    20821 gtctttgtcg tcattctcgg cagtattgcg atgcttagcc aggcggcgat ggccccagcg
    20881 ttattcatcc tcggtgccgc tggctttacg ctatatccgg tggcgatggc ctgggcttgt
    20941 gagaaagttg aacatcatca actggtggcg atgaaccaag ccttactgtt gagctatacc
    21001 gtgggaagtc tgcttggccc gtcatttacc gctatgctaa tgcagaattt ctccgataat
    21061 ttattgttta tcatgatcgc cagcgtatcg tttatctatt tgctgatgct gcttcgcaac
    21121 gccggtcata cgccgaaacc cgttgctcac gtgtaaatga attcaagcaa agtgtgaact
    21181 tactgtttca cactctgctt ttttgtttct tctatctgac ttgctttttt ccaaatttta
    21241 ttcgtttaaa aataaaatgt gcagcaggtt ataattttgc atttcgctat ttccgcactt
    21301 cttatttgcc gcgcataatc cctcgtttta cagatgcccc tttaattttg gcgaaggatt
    21361 tgtctatggc tgggaatgtt caggaagtaa gcgccccatc a
//
LOCUS       DADLTF010000066        21239 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.066, whole genome shotgun sequence.
ACCESSION   DADLTF010000066 DADLTF010000000
VERSION     DADLTF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.066"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(32..1012)
                     /locus_tag="JN749_002245"
     CDS             complement(32..1012)
                     /locus_tag="JN749_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HAY7368645.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="JN749_002246"
     CDS             complement(1020..1730)
                     /locus_tag="JN749_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HAY7368646.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1946..3769)
                     /gene="typA"
                     /locus_tag="JN749_002247"
     CDS             complement(1946..3769)
                     /gene="typA"
                     /locus_tag="JN749_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HAY7368647.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4142..5551
                     /gene="glnA"
                     /locus_tag="JN749_002248"
     CDS             4142..5551
                     /gene="glnA"
                     /locus_tag="JN749_002248"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HAY7368648.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5724..6773
                     /gene="glnL"
                     /locus_tag="JN749_002249"
     CDS             5724..6773
                     /gene="glnL"
                     /locus_tag="JN749_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HAY7368649.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6785..8194
                     /gene="glnG"
                     /locus_tag="JN749_002250"
     CDS             6785..8194
                     /gene="glnG"
                     /locus_tag="JN749_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HAY7368650.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8306..8416
                     /gene="yshB"
                     /locus_tag="JN749_002251"
     CDS             8306..8416
                     /gene="yshB"
                     /locus_tag="JN749_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HAY7368651.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="JN749_002252"
     CDS             complement(8645..10018)
                     /gene="hemN"
                     /locus_tag="JN749_002252"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAY7368652.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="JN749_002253"
     CDS             complement(10207..10716)
                     /gene="yihI"
                     /locus_tag="JN749_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HAY7368653.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            11298..11930
                     /locus_tag="JN749_002254"
     CDS             11298..11930
                     /locus_tag="JN749_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAY7368654.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12312..15098)
                     /gene="polA"
                     /locus_tag="JN749_002255"
     CDS             complement(12312..15098)
                     /gene="polA"
                     /locus_tag="JN749_002255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAY7368655.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYTEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15100..15342
                     /locus_tag="JN749_002256"
     CDS             15100..15342
                     /locus_tag="JN749_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368656.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSAGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMMFVFIFHAVKNKSIPNN"
     gene            15462..16394
                     /locus_tag="JN749_002257"
     CDS             15462..16394
                     /locus_tag="JN749_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7368657.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16435..17865)
                     /locus_tag="JN749_002258"
                     /pseudo
     CDS             complement(16435..17865)
                     /locus_tag="JN749_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
     gene            complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="JN749_002259"
     CDS             complement(18020..18646)
                     /gene="dsbA"
                     /locus_tag="JN749_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HAY7368658.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="JN749_002260"
     CDS             complement(18663..19649)
                     /gene="srkA"
                     /locus_tag="JN749_002260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HAY7368659.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRQTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(19726..19995)
                     /locus_tag="JN749_002261"
     CDS             complement(19726..19995)
                     /locus_tag="JN749_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HAY7368660.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20065..20649
                     /gene="mobA"
                     /locus_tag="JN749_002262"
     CDS             20065..20649
                     /gene="mobA"
                     /locus_tag="JN749_002262"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HAY7368661.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20631..21158
                     /gene="mobB"
                     /locus_tag="JN749_002263"
     CDS             20631..21158
                     /gene="mobB"
                     /locus_tag="JN749_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HAY7368662.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5178 a         5346 c         5120 g         5595 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctgaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaacctgcc atcgctggca
     1921 ggttttttat gactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg gcgcggcgac
     1981 gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc acttctacca
     2041 gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata ggcggaacca
     2101 gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta ccggtcaggc
     2161 agtttacagt caggtcgtta gagcggctat gaataccgat aatctgacct tcgtaaactt
     2221 ctgcaccgtg accgaggaac agcttaccgc gatcctgcag accgaacagc gcgaacgcga
     2281 cagctttacc ctgaccgtta gagatcagta caccgttctg acgctgaccc acttcgcccg
     2341 gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg gaagtcatgg
     2401 tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag tcgagacgta
     2461 cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg cccagcgcct
     2521 gcatcacaga accctgatgc tgttcttcaa cgtccagcgt cacgttttca tacggctctt
     2581 gtttacgacc gtcgatttca cggaagatta ctttcggacg ggataccgcc agttcgaaac
     2641 cttcacgacg catgttttcg ataagaacag agaggtgcag ttcaccacga ccagaaacgc
     2701 ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt accagttctt
     2761 tgttcagacg atcgaggatc tgacgagacg ttacgaactt accttcttta ccgcagaacg
     2821 gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag agtgccggca
     2881 gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg cccaggcccg
     2941 tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc agaccgaggt
     3001 ggcccagcac tttaccgact ttcgcgttac gggttttgcc ttcgctatcg atgatagtaa
     3061 cctgctggtt cggcttcact ttaccacgct tgatgcggcc aatgccgata acgccaacat
     3121 agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg tcaacgtccg
     3181 gcgcaggaac gtggtcaaca atcgcctgat acagcggggt catgtcttcc gccatatctt
     3241 cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg aagtccagct
     3301 gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca acccaatcag
     3361 gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg ccgtaagcaa
     3421 acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg tcaaccacca
     3481 gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag tcggcgtgcc
     3541 ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg tttttcgcga
     3601 ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc tcttgggttt
     3661 cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccaag gtggttttac
     3721 catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca actttgcctc
     3781 aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa caggatcaca
     3841 aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac ggagcataaa
     3901 aagggttatc caaaggtcat tgcaccaata tggtgcttaa tgtttccatt gaagcactat
     3961 attggtgcaa cattcacatc atggtgcagc ccttttgcac gatgttaagc atgataacgc
     4021 cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta cggcgacacg
     4081 gccaaaataa ttgcagattt cgttaccacg acgacaatga ccaatccagg agagttaaag
     4141 tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt ttgttgattt
     4201 gcgcttcacc gataccaaag gtaaagaaca acacgtcact atccctgctc atcaggtgaa
     4261 tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg gctggaaagg
     4321 cattaacgaa tccgacatgg tgctgatgcc agacgcatcc accgcagtga ttgacccgtt
     4381 cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca ccctgcaagg
     4441 ctatgaccgt gacccgcgct ctattgcgaa gcgcgccgaa gattacctgc gttccactgg
     4501 cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg atgacatccg
     4561 tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag gcgcatggaa
     4621 ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga aaggcggtta
     4681 cttcccggtt ccgccggtag actctgctca ggatattcgt tctgaaatgt gtctggtgat
     4741 ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg ctggtcagaa
     4801 cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc agatctacaa
     4861 atatgtcgtg cataacgtag cgcaccgctt cggtaaaacc gcgaccttta tgccaaaacc
     4921 gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta aaaacggcgt
     4981 taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt actacattgg
     5041 cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca ccaactctta
     5101 taagcgtctg gtcccgggct acgaagcacc ggtaatgctg gcttactctg cgcgtaaccg
     5161 ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta tcgaagtacg
     5221 tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga tggccggtct
     5281 tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc tgtatgacct
     5341 gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag aagcactgaa
     5401 cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcacgg atgaagcgat
     5461 cgatgcgtac atcgctctgc gtcgcgaaga agatgaccgc gtgcgtatga ctccgcatcc
     5521 ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc cggcgcacaa
     5581 cgccgggatt tagttgccgt ggaaactttc agcccatctc tgcatgggct tttttctccg
     5641 tcaattctct gatgcttcgc gctttttatc cgtaaaaagc tataatgcac taaaatggtg
     5701 caacctgttc aggagactgc tttatggcaa caggcacgca gcccgatgct gggcagatcc
     5761 tcaactcgct gattaacagt attttgttaa ttgatgacaa tctggcgatc cattacgcca
     5821 accccgccgc gcaacaactg ctcgcccaaa gctcccgcaa attgtttggt acgccgttac
     5881 cggaactgtt gagctacttc tcattaaata tcgagctgat gcaagaaagt ctggaggcgg
     5941 ggcaaggctt taccgataac gaagtgacgc tggtcatcga tggtcgctcg catatccttt
     6001 ctgtgacggc ccagcgtatg ccggacggca tgatcctgct ggagatggca ccgatggata
     6061 accagcgccg cttaagccag gaacaactac aacatgctca acaagtagct gcccgtgatt
     6121 tagtgcgcgg cctggcacat gagattaaaa atccgcttgg cggtttacgt ggcgcggcgc
     6181 agttactcag caaagcgtta cctgacccat cactactcga atataccaaa gtgattatcg
     6241 aacaggcgga ccggctgcga aatttggtcg accgtctgtt ggggccgcag ctgcccggta
     6301 cgcgcgttac cgaaagtatt cacaaagtgg ctgaacgcgt ggtaacgctg gtgtcgatgg
     6361 aactgccgga caacgtgcgg ttgattcgtg attacgatcc cagcctgccg gaactggcgc
     6421 acgacccgga tcaaattgaa caggtcttgc tgaatattgt gcgcaatgcg ctacaggcgc
     6481 tggggccgga aggcggtgaa atcattctgc gtacccgcac cgcgtttcaa ctgaccttac
     6541 acggcgagcg ctaccggctg gcggcgcgga ttgatgtgga agataacggg ccgggcattc
     6601 cgcctcattt gcaggatacg ctgttttacc cgatggtcag cggccgcgaa ggtggcaccg
     6661 ggcttggctt atccatcgcc cgtaatttga ttgatcagca ttcaggcaaa attgaattta
     6721 ccagttggcc aggtcatacc gagttctcgg tttacctgcc tatcaggaaa taaaggtgac
     6781 gtttatgcaa cgagggatag tctgggtagt cgatgacgat agttccatcc gttgggtgct
     6841 tgaacgtgcg ctcgctggag cgggtttaac ctgtacgaca tttgagaacg gcgcggaagt
     6901 actggaggcg ctggcgagca aaacgccgga tgtgctgctt tcagatatcc gtatgccggg
     6961 aatggacggg ctggcgctgc tcaagcagat taaacagcgc catccaatgc ttccggtcat
     7021 cattatgacc gcacattccg atctggatgc tgccgtcagc gcctatcaac aaggggcgtt
     7081 tgattatctg cccaaaccgt ttgatatcga cgaagcagtg gcgctggttg agcgcgctat
     7141 cagtcattac caggaacagc agcagccgcg taatattcag cttaacggcc caacgaccga
     7201 tatcatcggc gaagcgcaag ccatgcagga cgtgttccgt attatcggtc ggctttcgcg
     7261 ttcttctatt agcgtgctga ttaacggcga atccggcacc ggtaaagaac tggtcgctca
     7321 tgccctgcat cgccacagtc cgcgcaccaa agcgccgttt atcgcgctga atatggcagc
     7381 tatcccaaaa gatttgatcg aatcagaact gtttggccac gagaaaggcg cgtttactgg
     7441 cgcgaatacc attcgtcagg ggcgttttga acaggccgat ggcggcacgt tatttctcga
     7501 cgaaattgga gatatgccgc tggatgtgca gacgcgtttg ctgcgcgtgc tggcagacgg
     7561 tcagttttat cgcgttggcg gctatgcgcc ggtgaaagtg gatgtgcgga ttatcgctgc
     7621 cactcaccag aatcttgaac agcgggtgca ggaaggtaag ttccgtgagg atctgttcca
     7681 ccgcctgaac gttatccgcg ttcatctgcc gccgctgcgc gaacgtcggg aagatattcc
     7741 ccgtctggcg cgccattttt tacaggttgc cgcgcgcgaa ctgggcgtag aagcgaagtt
     7801 gctgcatccg gaaaccgaag ctgctctgac gcgtctggcg tggccaggca acgtgcgcca
     7861 gctggaaaac acctgccgct ggctaacggt gatggccgcc gggcaggaag tgttgattca
     7921 ggatttgccc ggcgaactgt ttgaatcaac ggttgcggag agtacttcgc aaatgcaacc
     7981 ggacagttgg gcgacacttt tagcgcagtg ggcagacaga gcgctgcgtt ccggtcatca
     8041 aaatctgctt tccgaagcgc agccagagct ggagcggacg ttactgacga ccgcgttgcg
     8101 acatacgcag gggcataaac aggaagcggc gcggctactc ggctggggcc gcaacactct
     8161 gacgcgtaag ttaaaagagc tggggatgga gtgattcacg gcttgtgtgt aaagattgat
     8221 tattgagcgc aaattgctgg tattttgcgc tttactgttc cgataagttc agtatgatct
     8281 tgcccggaaa acggggagag tcattatgct ggaatcaata attaatctgg tatcgagtgg
     8341 cgcagttgac agccacacac cgcaaactgc cgttgctgcc gtgctgtgtg ccgcgatgat
     8401 tgggctgttt agctgaagct cgttgtttat gccggatgcg gagaaagtcg taggcctgat
     8461 aagacgcgac cagcgtcgca tcaggcatca tgctcagatg cctgatgcgg cgtaaacgcc
     8521 ttatccggcc tacaaaagca ataatttcaa caggttttgt tgcccagcgc atcaggcaat
     8581 cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa gcgccattca
     8641 ctctttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga taggtatcaa
     8701 agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt attcccttct
     8761 catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga tcttccgcaa
     8821 agtaatcagc gaagtgcaaa tcccactgtt tctcaagagg ggcgtaatcc agacggaagt
     8881 tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt agcgcaatac
     8941 cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc aactctttct
     9001 ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc cccagcagat
     9061 cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct tcacgctggg
     9121 ccaccgccag ctcgtcatca ggacgggcaa agtgatccat accgataaac tgatagcccg
     9181 attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc ggactcggca
     9241 ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc gcgtagttaa
     9301 aaacgctcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag gcgaaactct
     9361 ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta aaaccaatct
     9421 cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg ttaaccagac
     9481 gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag ccttcggcgc
     9541 gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc gaaatctccg
     9601 catcggtatt gaaatggaag ttttcgcgca gcagcttcat cagacggctg atttgcgctt
     9661 tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga cgcccggcga
     9721 acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga tcggccttgt
     9781 gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta tggcagaacg
     9841 ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct tgtaaaaacg
     9901 cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg tatcgtggcc
     9961 cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt acagacatgc
    10021 tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc cgtttcgggt
    10081 catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt taacgaataa
    10141 ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc ccacggcggc
    10201 aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt cttcttcctc
    10261 ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa tacgatccag
    10321 tttagcatcc acccaggatt gctcttcggc gctcagggtt tcgcctgctt ccagacgttc
    10381 cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct gcggtgaaag
    10441 cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca ctcccaatgg
    10501 aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt tgctgcctga
    10561 cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt ttttctgacg
    10621 cttacggtca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgcg cttttgcatg
    10681 acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt tagccgtttt
    10741 attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa aggcgacaga
    10801 ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa tccttgcagg
    10861 ccgccaacac accctgtttt tccattagcc ggaacaatcc gtgttgattc catttccgtt
    10921 taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct cctggcgctc
    10981 ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg tgctacttta
    11041 cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt cagagtttta
    11101 tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga attttctctg
    11161 ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt acttacaacg
    11221 ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca aagctttgac
    11281 tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt gtgatgagtg
    11341 cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt gcaggccgtt
    11401 ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc ctggctcgta
    11461 cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct gacggcaagc
    11521 gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg aagcgcaaat
    11581 ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt ctggtggtgc
    11641 taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag tgggcggtag
    11701 acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca agcggcgcac
    11761 gtaaagcgct attgaatatg gtgcgtgaag ctgtactggc gtttaacggt gatgtgcagg
    11821 ttgaaacgtt ttcttcgttg aagaaacaag gcgtagacaa gctgcggcag aaactggata
    11881 cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa ttttcttgcc
    11941 ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt acaattctac
    12001 cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg cccaataaaa
    12061 aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc gattacgtga
    12121 agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact aactttactc
    12181 ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg cataggaatt
    12241 tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa aagggcatgt
    12301 tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc caccagcaac
    12361 ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc gacggcatca
    12421 acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat catacgtaca
    12481 cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt gataatgtcg
    12541 gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc acgacgagca
    12601 ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt ttcaacgtag
    12661 cccagctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc agggtagcgt
    12721 tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa ttgccgcgcc
    12781 agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc gctacggcgt
    12841 tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt tgcccggtgg
    12901 atatcttttc cttccgcgaa tgcggtcagc aagcctttat cacgcgagag atgcgccata
    12961 atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc tggcgcaata
    13021 aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg caggttagga
    13081 tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt atgcacacgc
    13141 ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aagtcgactt cagcttcgcc
    13201 agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag ttcttccagt
    13261 acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt aataccctgt
    13321 ttttcaaaca gaatggtttg caactgcttg gtggaagaaa ggttaaattc ttcacctgca
    13381 atttcatgcg ctttcttttc cagctcagcc agacgcaagg tgagctcttc agaatgattg
    13441 tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag caccggcacc
    13501 agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg cagatccggc
    13561 cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta acgtccggcc
    13621 tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttacccgc aatctcttca
    13681 aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc gtgacgcccg
    13741 gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc acgcagttca
    13801 atgccgtaat tcgccagaat accgcgatcg tatttcaggt tttgcccgac cttcagcgcc
    13861 ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgtgaga gatttgatcg
    13921 ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg ctcgatagca
    13981 aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc ggtatcaaat
    14041 gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc ttcatcaagg
    14101 atagtgacgt agttgtcata agaaatcacc gttgccgtca cttctggcgc ttcgtctgca
    14161 acactggttt cctgcggctt cgcggctggt tttgcccctt tggcctgtaa ccatttgccc
    14221 gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc caacaactct
    14281 tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc aacatcggtt
    14341 ttaatcgtcg ccaactggta tgagagataa gcaacttctt tgttttgctc gagcttcgct
    14401 gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc ggtatacagc
    14461 gtatccagcc cgccaagacc ttgcagcaat gcctgcgcgg ttttttcacc gacgcccggt
    14521 acgccaggaa tgttatcaga ggagtcaccc atcagcgcca ggaagtcgat gatcaattct
    14581 ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt cgtcatggta
    14641 ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc agtgctaatc
    14701 agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac gtcgtccgct
    14761 tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc gtgcagaggt
    14821 tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta atgttcaaac
    14881 agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg cgtcggttta
    14941 tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc ggtcggctcg
    15001 cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata agatgaacca
    15061 tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc tgtttatcag
    15121 attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc gcttttcgtg
    15181 aaaatctttt gtagatctgc tggatcgctc gcaaatcgtc atgtggataa ctttgtgcat
    15241 aaattattta catcgctttt aatttattat catgcttttt acagcatgat gtttgtcttt
    15301 atttttcatg cagttaaaaa taaatccatc cctaataatt gagataatgg gattttttct
    15361 aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt gggtcatttt
    15421 ctgctaaaat ctgcgccttg cgcttcttta gtgcactgtc tatggctaac cttttgaata
    15481 aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc gtattgacca
    15541 ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta aaactattgc
    15601 tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg atgtattgct
    15661 ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg gaagttcacg
    15721 ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac cgtagttggg
    15781 cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat aaatatttcc
    15841 tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg ctggatatgc
    15901 cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga cgtggtcaag
    15961 atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc accattgtca
    16021 atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac tccacttttc
    16081 aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta ctgggtaaac
    16141 aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt cagccgttct
    16201 tcgatatgtt aagcggtaaa ctgacgcgga ttgtcgtaca tgtggattta cagcctattg
    16261 ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat tttcagcaat
    16321 ggctgaactc attgtggcag gaaaaagacc ggcttctgac gtctctcata tcatcacagc
    16381 gtcagaataa gtaagtttta ctcgcctctg aatgagcaga ggcgagtgag tattttaatg
    16441 aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc cgttaaaaac
    16501 aactttaccc ggtgtataac gcagctgcag cgaggcggta ttgttgtcaa tcgtgattaa
    16561 ctgaaacatc tgacctaatg tcatcatccc gctgatttgt ttatcagcct gcttttgcgc
    16621 tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttatg tgacgacatt
    16681 aagcggtaac ttcacatcaa aattgagcga tttgatatct ttgtttgtgg atgatgaaga
    16741 tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga gtgcgttctt
    16801 ccatcttacc ggttgtttaa tggtcggctc acttttttgt aataacggta aatactcttt
    16861 gaacaatgca gcattcactt cttgcagagc aacttcatcg tttgctaact cagggtgtgc
    16921 agcaagctgc ttctgcatcg caatgttata ctggatgata aattggcgca ctgctgaggg
    16981 atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat tcccttttgt
    17041 taacttatca agttcgtagg tcagattgac attgatataa tctttgtctt taacgcgatc
    17101 cagcctggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt gattgataat
    17161 gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt cttctaatcg
    17221 cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg ataattgcag
    17281 aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac catcaccatt
    17341 aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg caacatctaa
    17401 tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg gcgatttatt
    17461 tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actacgttca cttttgccgc
    17521 tgccaatgct gggatgacat taccatgcat taacattgtg atgggcaacg gaccgtgctc
    17581 aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg gtgcaccgtt
    17641 atcgaatgtc atttgcattt gaatacggga attaaaaaag cctttgtcaa agtttttata
    17701 acttaacgtc atatcgtagg catgttcacc tttctttttc gcatcgttaa aatctttaat
    17761 aaacttttcg ataccaggct gaatttgcgt acctgtgtac catgtgccac caccccagat
    17821 tacggctaac gcaacaataa cacctgtagc tgacttacgt atcatataaa tattccctga
    17881 taaaacagca aaactttgaa tatccactta tgctgaaata atgtccagcg gcaggatgca
    17941 ttatcagaat gtatttatat ttattaaata ataaaaaaag cccgtgaata ttcacgggct
    18001 ttatgtaatt tacattgaat tattttttct cggacagata tttcaccgta tcagcatact
    18061 gctgaacaaa aacatccata ttgctggtat ccataccctg cggattcagc tgatatttac
    18121 cgttaacaaa catcgccgga acaccacgca attgcacatc agctgcggct ttttcctgct
    18181 gagcgaccag agatttcacc acgaagctgt tccacgccgc gtcgtactct tcacctttaa
    18241 tacctgcatt gataaataca tcgcggatat cagatgctga acgaatggtc tgggttttct
    18301 gtacgccttc aaacagcgga acagtcactt tgtcttccac gcccagcgcc atcgccacag
    18361 cccatgcctg agtcagatct ttgcccaggt caccacccat gaagttgacg tggtatttag
    18421 tcatcttcac gccttccggc agttttttct tcacattatc agaaatatgc agaacttctt
    18481 caaactgata gcagtgcggg cagaagaaag agaaaaactc cagcacttgc ggcgcgccag
    18541 ctaccggttt ttccagggta gtgtactgtt taccatcttc atactgcgcc gccgatgcgc
    18601 taaacgctaa aactaaacca gccagcgcca gccaaatctt tttcatgatc tactctctcc
    18661 gattaataca taggtgttaa ttgcaaaggg ggttcttgta gaacttttgc ctgttctata
    18721 aaagtcgccg tctgtcgtag ccagtaatct tccccggtta accacgggaa atttttcggg
    18781 aacgcgggat cagcccaatg ccgcattagc caggcaagat aataaaccaa acgcatggcg
    18841 cgtaaaggtt caatcagtcc gatttcagcg gtgtcgaact cgctaaattc ttcataagct
    18901 tcaataatag tttccagttg catccgctgc tgggctttat cgccattgag caacatccac
    18961 aaatcctgaa tagctggacc attacgtgca tcatccagat caacaaacat tggaccatcg
    19021 cgccagagaa tattcccggc gtggcagtct ccatgtagcc gcagaacggt gaaatcttcc
    19081 cgccagtgtg ctgtaacggc ggcgatcagc tcatctgtcg ctttcagaaa tgccgctttc
    19141 aacccggaag gtatcagtgt agcgtcctca aacagcttgc gaggctcaat gagatattca
    19201 ttcagaccga tggtctggcg atgaataaaa agctgtttgc gccccgtctg gtgcatacgc
    19261 cctaaataac gcccaaccgc ttccatctga tcgatattat cagcttcgaa ctggcgacca
    19321 ccgacgcttg gaaaaacagc gaaataaaat ccctgatgat tcaataaagt ctgaccgtta
    19381 aaggccacag gtgctgcgac cggaacttca tcatttacca gctgcaacgc aaattgatgt
    19441 tcttcgagaa tttgatcggc tgtccaacgt tcagggcgat aaaatttgac gacaaaacgt
    19501 cgacgctctt cgtcctgaaa ttgatagaca cggttttcat agctgttaag cggggtaaga
    19561 ccggaatcca cccggatccc ttgctcaaac agagcgtcca tgatggtatc cgggtgtagt
    19621 gtctggaaag taaaagcgct gttgttcatc ccatcatccg gaaaacatta cgaatgatga
    19681 aggatatcat ttcacgatga ttttggtggc gccgcttaca agcttttact ctttaattac
    19741 gccgcgagcg cgtaacagcg ccgttttgaa gtcttcttca taatctttct gcaaacccgg
    19801 gatcaccgca tctttcgcgg aatcacgcat tttcaggtga tagatcagaa tgtcatccgt
    19861 caaatccgcc agttcgccgt caaaacctga ctctttcgcc agtttctgca aaaattgcaa
    19921 caagttaaag tctggctctt tttgccaggc tggctggagg agttcaataa cttcattcag
    19981 acgtttacat ttcatagtaa tgctccttac tcttgagaca gacacgttag cagggtcaat
    20041 cccacaataa aagaggcgat atcggtgaat ctgatgacga cgataacagg cgttgtgctg
    20101 gcaggcggta aagccagacg aatgggcggc gtagataaag gattgctgga cttaaatggc
    20161 aaaccgctgt ggcaacatgt cgctgacgcg cttaagacgc agctctctca cgtcgtggtt
    20221 aatgctaatc gtcatcagga aatctatcag gcaggcggtc tgaaagtgat tgaagattca
    20281 ctggcggatt acccaggccc tctggcagga atgctttcag taatgcagca ggaagctggt
    20341 gagtggtttt tgttttgtcc gtgcgatacg ccttacattc cccatgattt agccgcccgg
    20401 cttaatcatc agcgcaaaga tgcgcctgtc gtgtgggtcc atgacggtga acgcgatcac
    20461 ccgactattg ctctggtaaa ccgcgctatt gagcctttat tactggaata tctgcaagca
    20521 ggagaacgcc gggtaatggc atttatgcgt ctggctggcg gtcatgcggt tgatttcagc
    20581 gatcataaag atgcatttgt taacgtgaat acgccagagg agcttgcccg atggcaggaa
    20641 aaacgatgat accgttactc gcctttgccg cgtggagtgg taccggaaaa actacgcttt
    20701 tgaaaaaact gatcccggca ttatgcgcca gagggatccg tccggggctg attaagcata
    20761 cgcaccatga tatggatgtt gataagccag gcaaagatag ctatgagctg cgcaaggctg
    20821 gcgcggcaca aaccatcgtt gccagccagc aacgatgggc cttgatgacg gaaacaccag
    20881 acgaagaaga gctggatcta cattttctcg caagtcgaat ggatacctca aagctggatt
    20941 tgattctggt cgaagggttt aagcatgaag agatcgcaaa gattgtgctg tttcgcgatg
    21001 cagccggaca tcgacctgaa gaattagtga tagacaggca tgttattgct gtagccagtg
    21061 atgtgccgct taatcttgat gtcgcattac tggatattaa tgatgttgag gggctggctg
    21121 attttgtggt ggagtggatg caaaagcaga acggataagc aagagtaggc tggatcagac
    21181 gcagccagtg tcgcatccga cctttttctg caccagcgca aaaaggcccg actgggcct
//
LOCUS       DADLTF010000067        21039 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.067, whole genome shotgun sequence.
ACCESSION   DADLTF010000067 DADLTF010000000
VERSION     DADLTF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21039
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.067"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..3088)
                     /locus_tag="JN749_002264"
                     /pseudo
     CDS             complement(1..3088)
                     /locus_tag="JN749_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3386..4579
                     /locus_tag="JN749_002265"
     CDS             3386..4579
                     /locus_tag="JN749_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAY7368663.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4777..6030)
                     /locus_tag="JN749_002266"
     CDS             complement(4777..6030)
                     /locus_tag="JN749_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAY7368664.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6357..7547
                     /gene="hmpA"
                     /locus_tag="JN749_002267"
     CDS             6357..7547
                     /gene="hmpA"
                     /locus_tag="JN749_002267"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HAY7368665.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7592..7930)
                     /gene="glnB"
                     /locus_tag="JN749_002268"
     CDS             complement(7592..7930)
                     /gene="glnB"
                     /locus_tag="JN749_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HAY7368666.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7991..9325)
                     /gene="glrR"
                     /locus_tag="JN749_002269"
     CDS             complement(7991..9325)
                     /gene="glrR"
                     /locus_tag="JN749_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HAY7368667.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9315..10028)
                     /gene="qseG"
                     /locus_tag="JN749_002270"
     CDS             complement(9315..10028)
                     /gene="qseG"
                     /locus_tag="JN749_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HAY7368668.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLEKLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10193..11620)
                     /gene="qseE"
                     /locus_tag="JN749_002271"
     CDS             complement(10193..11620)
                     /gene="qseE"
                     /locus_tag="JN749_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HAY7368669.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12178..16065)
                     /gene="purL"
                     /locus_tag="JN749_002272"
                     /gene_synonym="purI"
     CDS             complement(12178..16065)
                     /gene="purL"
                     /locus_tag="JN749_002272"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HAY7368670.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16323..17879
                     /gene="mltF"
                     /locus_tag="JN749_002273"
     CDS             16323..17879
                     /gene="mltF"
                     /locus_tag="JN749_002273"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HAY7368671.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17876..18412)
                     /gene="tadA"
                     /locus_tag="JN749_002274"
     CDS             complement(17876..18412)
                     /gene="tadA"
                     /locus_tag="JN749_002274"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAY7368672.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18437..19072)
                     /gene="yfhb"
                     /locus_tag="JN749_002275"
     CDS             complement(18437..19072)
                     /gene="yfhb"
                     /locus_tag="JN749_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HAY7368673.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19281..20129
                     /locus_tag="JN749_002276"
     CDS             19281..20129
                     /locus_tag="JN749_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAY7368674.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20468..21022)
                     /gene="pinE"
                     /locus_tag="JN749_002277"
                     /pseudo
     CDS             complement(20468..21022)
                     /gene="pinE"
                     /locus_tag="JN749_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
BASE COUNT         5054 a         5684 c         5353 g         4948 t
ORIGIN      
        1 acgtagttaa aaataaaagg catcacagtg gtgaataatg ccgctttcgg ccaggccgga
       61 ttaatcgccg tcaattcagc gcgagcagac tcatattgcg caacctcccc gagtcccgcg
      121 gacgccagca tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgccatac
      181 agcgataaca ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc
      241 atctcttgcg cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc
      301 agcagtcgca gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa
      361 ctgtggatgt taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca
      421 gtttcatcgc cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt
      481 tgccccggta aacggccttc gcttaaattc tccggataat gcagcgcggc atgaagcagt
      541 tcgctggcct gctgcggctc tctggcatca agatgctgcc aggcgcgtaa taactggttg
      601 aggataaact gactggtgac cttcccttcc ccgccttccc acgggtggaa tttacgcgtg
      661 gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct
      721 gcggtcatgt cgtcgcgttt cagcgcaatt tccagattat tttccagacg cgccagtcgt
      781 ttctccggtg tggctccact taacttatcc agcaaatccc gttcgaaaag cagacgtgca
      841 tcctgcggcg caagctgata agcattatca agataacgcg cggccagctc ataatcgtgt
      901 ttcttattcc acgcatggat cgctaacccg cgccagccgt cggcaaactc cggcgacatc
      961 tctacgcaac gttgccataa ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag
     1021 caggccagta aatggcgagc aaaccagcac tcttcaatac tctccagcgc cgccacttct
     1081 tccagcgtat tcgggaaacg gacaaactgc gggaagacat caatggcttt tgcgaccagt
     1141 tcgccacgtt cggctttcgg cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg
     1201 cagtccagtg cgttcagcat ctctgccgcc agagtgggca ttccccagtt aatcagttgc
     1261 ccggcggtca tcagagcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg
     1321 gctgattcgc tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa
     1381 tcacgcagca gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagc
     1441 agcagattat gcaagcaaag cacttcctga ttggttgggc aggcacgaag actttgttgg
     1501 caaaaatcca gacccgcgtc gaagttatca ttacgcgccg ccagtcgtgc cagaccataa
     1561 tagccgcctg ctttactatt gccgctccag accgcacgcc agaaatcctc ttcggcttgt
     1621 tgatattgtc cctgacgttc gtaagcactg gcgcgaatca aacttgcttg tccacactgc
     1681 gggtttttgt tcagcgcatg tgcgcgtcct gactggcata cgccaccgct tgcgggaaat
     1741 ctgcgcggtt atattccagc atcgccagcg ccaggttaca gcgataatcc agcgggtcca
     1801 gcgccacgcc gcgcaggtag taatcgaacg gtgaacggct ggcgtgatga tattgctcca
     1861 gatgctgacc gataaaccag gcttcatctg tactggtaat gtcttgtgct gccagtggcg
     1921 ctttggcgac gtccggcaac ggcaactcct gcggctgatg ttcctgataa ctcagtacaa
     1981 tattgccgtc ggcatcggag agttcaatgg tcagtctgtc aggattgata ccgtgcaaca
     2041 cgccctggat ggcggtcgct ggtgtcaggg ccacggcatc atcgagcaac gcgttgcatt
     2101 tgccgatttc gcggatcgcc agacggtatc cgttcaacgg agagatggca tacagccccc
     2161 actcaatccc ccgctcacta cgctggagtt ttatcaccgc atcgcgggag gcattttgca
     2221 ccatgcacag agaatgataa ggcaggaaat actgctcgaa acgcttctct tcgtaagcat
     2281 caagccaggt aaaatcaggc tggttatcgg caaaaatacc ggtcatcagt tcgatatacg
     2341 ggccgttatt gtcggtcaga ctcttatccc acgcctggcc aaattcactg tgtccccaac
     2401 tccactgttt tttacctggc gcaatatggt ggttggcaac gtgcagcaaa ccgccatctt
     2461 catcgtgaca ccacgcgccg acaaaatcgt actgcgattt ttcggccata tatgaggttg
     2521 gaacaggcac atttttatag cgagaaatgt ccactccggc ggagtagtcc actttgtagt
     2581 aagtgccggt ggcgatgggg aaagcggaga cggcccgttt gccgtgatca aacaccgcag
     2641 tcacatccgg tgggaaaacg ctctgatgcc cttcaccccc tttcactgcc gggttggccc
     2701 accacaagaa atgacgcggc gtggcgttgc cgttatagac gcggctggcg atttccagtg
     2761 ccgcccggtc agggcgcagg gtgaaacctg tcatcacctg taaaccatgc atcggctccg
     2821 tttcgccgac ccacaccgtc tgtgcaccgt cttcatgggc ttcgagggtg aaatcaacgg
     2881 gcataaaggt ggtcgggcga tggtgttgcg gccagttaaa ctcaatcccg ccggagatcc
     2941 acggtcccag cagccccacc agcgcaggtt taatgacttc attgtgatag acaaaatcgc
     3001 gctgtttcac tttatcccat gcgcgatgca cccgaccgcc cagttccggc aggatcatca
     3061 ctttgatgta gtcgttttcc agccacaccg cctgctagga tttcagggtt ttctgctcgc
     3121 tcagcgtatc ggtcacgccg tagggataaa ccgcgccgga cgatccctga taaacgcgat
     3181 tttccaggaa catgggatgt atatcctgcg gcccggtttc ataggtcggg atctcaacgc
     3241 gctcttgcca cacttttact ggagtcatgg tgccctcaat attaacaaga catactgaat
     3301 taaaagattt gtggcagtgt attgaacaat ctggcaatgt tttcgcggaa taatcacgca
     3361 attaactaaa caaggtttag tgaagatgag agcctgcatt aacaatcaac agattcgcca
     3421 ccataacaaa tgcgtgattc tggaactgct gtaccggcaa aagcgcgcca ataaatcaac
     3481 gctggcccgg ctggcgcaaa tttcgattcc ggcagtcagt aatattttgc aggaactgga
     3541 aagcgaaaaa cgggtggtga atattgacga tgaaagccag acgcgcgggc atagtagcgg
     3601 tacatggctg attgcgccgg aaggtgactg gacgctgtgc ctgaacgtga cgcccaccag
     3661 tattgagtgt caggttgcta atgcttgttt aagtccgaaa ggcgaatttg agtatttaca
     3721 gattgatgta ccgacgccgc aggcgctgct gtccgaaatc gaaaaatgct ggcatcgcca
     3781 ccgtaaattg tggccggacc gcaccatcaa tctggcgctg gcaatccacg ggcaggttga
     3841 tccggtgacc ggcgtatcgc aaaccatgcc gcaagcgccg tggacaacgc cagttgaggt
     3901 gaagtatctg ctggaagaga agctgggcat tcgggtgctg gtcgataatg actgcgtgat
     3961 gctggcgcag gcggagaaat ggcaaaataa ttcgcaggaa cgggatttct gcgtgatcaa
     4021 cgttgattac ggcattggct cgtcgttcgt gattaacgag caaatttatc gcggcagttt
     4081 gtatggtagc ggacagattg gtcacaccat cgttaatccg gatggcgtcg tctgcgactg
     4141 tggacgttac ggctgcctgg aaaccgtcgc ctcgttaagt gcattaaaaa aacaggcgcg
     4201 ggtatggcta aaatcacaac cggttaatac tcagcttgat cctgaaaaac tgacaaccgc
     4261 gcagttaatc gctgcctggc agagtggaga accgtggatc accagctggg ttgatcgctc
     4321 tgccaatgcc attggtttga gtctgtataa cttcctcaac atcctcaata ttaatcagat
     4381 ttggttgtac ggtcgcagtt gtgcctttgg tgagaactgg cttaatacta ttattcgcca
     4441 gacaggattt aacccgttcg accgcgacga aggaccgagc gtgaaagcga cgcaaattgg
     4501 cttcgggcaa ttaagccgcg cacaacaggt gctgggaatt ggctatttgt atgttgaggc
     4561 gcagttacga cagatttgat ggcgcgataa cgtagaaagg cttcccgaag gaagccttga
     4621 tgatcataaa cgaaaaattg cctgatgcgc tacgcttatc aggcctacac ggagattgca
     4681 atatattgaa tttgcaagat tttgtaggcc tgagaaggtg ttcatgccgc atccaacatg
     4741 aacaacgagc acattgtcag caaatcaccg tttcgcttat gcgtaaaccg ggtaacgtgc
     4801 gcagatgtcg agaactttac ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc
     4861 cagcacgtca cacatccagc cagccagttc tttcgcttca gcttctttaa agccgcgacg
     4921 ggtaatcgcc ggagtaccta cacgaatacc ggaggtcaca aacgggctct tcggatcgtt
     4981 cggtacgctg tttttgttga cggtgatgtt agcacggccc agagcggcgt ctgcttcttt
     5041 accggtcagg tttttatcaa ccagatcaac caggaacagg tggttatcag tgccgccgga
     5101 aaccactttg tagccgcgct cgaggaacac ttctaccatc gctttagcgt ttttcgcgac
     5161 ctgctgctgg taagttttga actcaggctc catcgcttct ttcagagcaa ccgctttacc
     5221 ggcgattacg tgcatcaacg gaccgccctg accaccaggg aaaacggcag agttcagttt
     5281 tttgtacagc tcttcgctac cacctttcgc caggatcagg ccgccgcgcg gacccgccag
     5341 ggttttgtga gtggtggtag taacaacgtg agcatgagga accgggttcg ggtagacgcc
     5401 agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac agataagcac cgatgctgtc
     5461 agcgatttca cgcattttcg cccagtcaac cacgccggaa tatgcagaga aaccaccgat
     5521 aatcattttc ggcttgtgtt ctttggcttg tttttccaga tcggcgtaat cgatatgacc
     5581 ggtagcatcg ataccgtaag gaacgatgtt gtacagttta ccggagaagt taaccggaga
     5641 accgtgagtc aggtgaccgc catgcgccag gttcataccc agaacggtat cacctggttc
     5701 cagcagcgcg gtgtagaccg caaagttagc ctgggagccg gagtgcggct ggacgttagc
     5761 gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac
     5821 atactcgcaa ccgccgtagt agcgtttgcc cggataacct tcagcatatt tgttggtcag
     5881 ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc
     5941 gatgtgctct tcctgacgta ctttttcctg ctccatagcc tgccacagtt cggcatcata
     6001 atcggcaatg ttcatttcac gctttaacat ccgcatctcc tgactcagct aacaataaaa
     6061 tttttggcct ttataggcgg tcctgttgga caacggcgaa cagtataacc gaatcattgt
     6121 gcgataacag gtcttgacaa aggaatttac gcaaacgatt accttaaggc tacgcaaggc
     6181 tttggagaat aaagagcttg caaccggaaa cggatttctt ttcaggtttg tgatgcaaat
     6241 ttttcacttc atcacattct ttctgaaaaa caccaaagaa ccatttacat tgcagggcta
     6301 ttttttataa gatgcatttg agatacatca attaagatgc aaaaaaggaa gaccatatgc
     6361 ttgacgctca aaccatcgct acagtaaaag ccaccatccc tttactggtg gaaacggggc
     6421 caaagttaac cgcccatttc tacgaccgta tgtttactca taacccagaa ctcaaagaaa
     6481 tttttaacat gagtaaccag cgtaatggcg atcaacgtga agccctgttt aacgctattg
     6541 ccgcctacgc cagtaatatt gaaaacctgc ctgcgctgct gccagcggta gaaaaaatcg
     6601 cgcagaagca caccagcttc cagatcaaac cggaacagta caacatcgtc ggtgaacacc
     6661 tgttggcaac gctggacgaa atgttcagcc cggggcagga agtgctggac gcgtggggta
     6721 aagcctatgg tgtactggct aatgtattta tcaatcgcga ggcggaaatc tataacgaaa
     6781 acgccagcaa agccggtggt tgggaaggta ctcgcgattt ccgcattgtg gctaaaacac
     6841 cgcgcagcgc gcttatcacc agcttcgaac tggagccggt cgacggtggc gcagtggcag
     6901 aataccgtcc ggggcaatat ctcggcgtct ggctgaagcc ggaaggtttc ccgcatcagg
     6961 aaattcgtca gtactctttg actcgcaaac cggatggcaa aggctatcgt atcgcggtga
     7021 aacgcgaaga gggtgggcag gtatccaact ggttgcacaa tcacgccaat gttggcgatg
     7081 tcgtgaaact ggtcgctcca gcaggtgatt tctttatggc tgtcgcagat gacacaccag
     7141 tgacgttaat ctctaccggt gttggtcaaa cgccaatgct ggcaatgctc gacacgctgg
     7201 caaaagcagg ccacacagca caagtgaact ggttccatgc ggcagaaaat ggcgaagttc
     7261 acgcctttgc tgatgaagtt aaggaactgg ggcagtcact gccgcgcttt accgcgcaca
     7321 cctggtatcg tcagccgagc gaagccgatc gcgctaaagg tcagtttgat agcgaaggtc
     7381 tgatggattt gagcaaactg gaaggtgcgt tcagcgatcc gacaatgcag ttctatctct
     7441 gcggcccggt tggcttcatg cagtttaccg cgaaacagtt agtggatctg ggcgtgaagc
     7501 aggaaaacat tcattacgaa tgctttggcc cgcataaggt gctgtaattt gatgttgccg
     7561 gatggaaaca tccggcaacc cttgacgcgg tttaaattgc cgcgtcgtcc tcttcaccgg
     7621 tacggatgcg aatgacccga gccacgtcaa agacgaagat tttaccgtca ccgattttgc
     7681 cggtttgcgc cgtgcgaata atggtatcga cacaggtatc gacaatgtcg tccggtacga
     7741 caatctcaat tttcactttc ggcagaaaat ccaccatata ctccgcgccg cggtacagct
     7801 cggtatggcc tttctggcga ccaaagcctt tcacttcggt caccgtcatg ccggtaatac
     7861 cgacttcggc cagtgcttcg cggacatcgt ccagcttgaa gggttttata atcgcatcaa
     7921 tctttttcat gctattcctt gaaaaggttg tctgtctttt gatctgctaa acgtaacaca
     7981 taacgccaat tcattccttg aaatcgtttg catccagctc gtgtcgggaa agcagtttat
     8041 aaaattctgt ccggttgcgc cccgccattc tcgccgcgtg ggtgacgttg cctttggtga
     8101 tttgcagcag cttacgcaaa tagttgagtt caaaatgatt acgtgcctca acaaaggttg
     8161 gcagcgccgt attttcaccc tccagcgcct gctccaccag cgcatcacta atcaccggag
     8221 atgaggtcag cgccacgcac tgttcaatca cgttgaccaa ctggcgcaca ttacccggcc
     8281 agctcgcggt catcaggcgt ttcatcgcat cggtagagaa cgcgcggaca aacggtttat
     8341 gtcgctctgc cgcctggcgc aacaggtgat ttgccagtag cggaatgtct tctgtgcgct
     8401 ccgccagtgc cggaattttc aggctgacaa cgttgaggcg gtaatagaga tcttcgcgga
     8461 attccccgcg cgccatcgct tttgacagat cacggtgagt ggcagaaata atccgcacat
     8521 tgatatcaat atcgcggtta ctgcccagcg ggcgcacttt acgctcctgc aacacgcgca
     8581 gcagtttgac ctgtaacggc gcaggcatat cgccaatttc atcgagaaat agcgttccgc
     8641 cttccgccgc ctggaataaa ccttcgcgat tgctgacagc gccagtaaac gcgccacgcg
     8701 catgaccaaa cagctccgac tccagcaatt gctcgggcaa tgcgccacag ttaatagcaa
     8761 taaatggttt gctgttgcgc gggctggcgt tgtggatagc ctgggcgaaa atctctttcc
     8821 cggtgccgct ctgaccgtta atcaaaacgc tgacgtctga ttgcgccacc agccgcgcct
     8881 gttccagcaa acgcagcatc agcgggctgc gggtgacaat tgcctcgcgc cagcgttcat
     8941 cggtggctgg cgcggattgc tccagcgcat cgtcaattgc ctgatatagc gcatctttat
     9001 cgacaggctt ggtgaggaaa ctaaaaacgc cctgctgtgt tgcagcaacg gcatcgggga
     9061 tagaaccatg cgcagtaaga ataattactg gcattcccgg ctgcactttc tggatttcag
     9121 caaatagctg cataccgtcc atttcatcca tccgcaggtc gctgataact aaatctactt
     9181 tttcgcgatt cagtacccgt aatccttcag cgccactttc cgccgtgacc acactgtagc
     9241 cttcgctggt caggcgcagg ccaagcagtt tcagcaatcc cggatcgtca tcgaccaata
     9301 ataaatgcgc aggtttatgg ctcatcagga gtgacctcat gggtggatgg cgcgggcttt
     9361 tcactctcgt gcggcgtatc cggcgagaaa tttccagccg gtttgcgggt cgagagctgg
     9421 cgttcaatat cggtcagttt ttccagcttg cgggtggtga gttccagttg ctgctgtaga
     9481 acgtgatgtt gctggcgcaa tgtatccagc tcgctgtcgc tggactgctg gagtttactg
     9541 taacgttggc gctcttccgc cagttgcagt tgcagcgcct gaccgtcgcg ccagagttga
     9601 tacagtgggc gaacctgtgc cgggatctcg gtacttaacg cttcaatacg cgcgaccagc
     9661 tggcggcgct catacggcgt aattttggcg tcggcgagca aaatcccttg tttaaaggta
     9721 ttttgccagc tgccgtcgtc atattggcga gcttgctgac gcgactgcgc aggcattaaa
     9781 cgatcagcac aatccatcgc ccgcagccag taaagcggat tggtttcggt tgatttacct
     9841 tgcagcgccc agatgtcgct acattcagta gaaagatagt cagccagttg ataaaccgga
     9901 attttttctt ctgctggcgt atcaatggct ggcttattgt gattctgcac gcaacccagc
     9961 aatgccagac atggcagccc tgccagccac agccgtcggg gcaataatcg ttgaaaaatg
    10021 tgtcgcatat tcaccagact taaagcctat cccagtgggc gtaattgttg cagacagtct
    10081 ggacatggac agcgcggaga aaccggggcg tacatatcgt acgtgaagat ttcgagcact
    10141 acccggggcc gaactgacaa ataaaatagc ctgataggat aggctgttag cattatttcg
    10201 tgtttttcga cgacggtaat tcaatgcgga aacaaacgtc ttgcccgctc tcgtcgacca
    10261 gatacagttc cccttgcata cggcgaatac aatccctggc aatgcttaat cccagaccgc
    10321 tgcccttcac cgcccctttt cgctggtggc ttccctgaaa aaagggttcg aagatcatgg
    10381 cgcgttcctc ttgcggaatg ggcgtgcctg tattgatgac atcaatataa acccgcgcac
    10441 catgtaaact gctgcgaagg caaatgttac cggattcagc cccgtagtgc accgcattgg
    10501 agtaaagatt atccagtacg ctcatcagca gcattggctc cgccaagcaa gctgttgctt
    10561 tgagatcgac gtcggtatgc atcattttag cccgtgcggg caggctatga gcagaaacca
    10621 ctgtctccac cagcggtgct aattcaacat tctccagttc caccgcactg tccgcctgtt
    10681 tacggttgta atcaagcagt tgttcgatca gtttttgcaa attgcagctg ctgctatcaa
    10741 gaatgctcac cacctctttt tgctctggcg taagcggccc gacaacctga tcagccagta
    10801 attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa ttcatgagat aaatgtctta
    10861 aaaattgatg gcgttgggat tccagccatg acaggcgctc acttaaccaa agaatacgtt
    10921 gcccaaccga gcgtaactcg ctcggtccac agaacgaaac gctattgccc agagaacgcc
    10981 cttcccccag acggttgatc atgcgctcga tatttttcac cggcccgata atcatccgcg
    11041 tgaaaagtag caccatcacc agactcacca gaaatagcac cagcgattgc caaccaaaat
    11101 attgcccacg ttcggcgatt tcacgctgaa gttgctgccc acgagagaac accactgtgc
    11161 gcgtggcctg caccatttcg gtattggcac tggcaaaggc ttcaagacgc gcggcggcgg
    11221 cagcatcggg accactgttg ttacactgaa gttgggccag attgttcaaa tcctgacgca
    11281 atgcctggta aagtttatcg tctggcagta cgcctgcgtg ggcatcgagc atttcgctgt
    11341 aacgcttgcg ctggctttga taaaccttcg caagcgtagg gtcgtccagc acgcaatact
    11401 gacggtagct acgctccatc tccagcgccg cgttggtcat cgcttcacta cgccgggcat
    11461 caataagcgt agtgcggtta accagcgccg cctgatcgct aagcgcattc aggctttgcc
    11521 aggcttgcca tgccagcacc aacaggggta gtagaattag caaaaatgcc agcattacca
    11581 gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa ggtgttactc tcgtcagacg
    11641 cgaatagcct gatgctaacc gaggggaagt tcagatacaa caaagccggg aattacccgg
    11701 ctttgttatg gaataaggcg gtgcctaact cgacgtttcg cccgatggtt gatatagcta
    11761 cgctgatatc agaagttgga cggcaggcac cttgttgtgc gtcattcgaa ttttatgtag
    11821 cacgtcccga aggggctgac ataagtcggt gaatgagcca ctgcttacta ttatgcagta
    11881 actgtgccaa taaagaaaat agtttggtaa cgtactgatt atacgttgct ttgagagatt
    11941 tatggcttct tcgctatttg aatgtaaatc agccactgtg tcgctaaaaa gagacaactt
    12001 aagataaact tattagatta caataaaatc aatcatttaa gtgtcgccag aaagcgacac
    12061 ggtaaaccac ccattgtcgt gatttacaga cacaaaaaag ctcccagagt tgggagctta
    12121 tgatagtggt tggtgcttaa tgccgcatcc gggctgcaaa accaatgaac tgatgactta
    12181 ccccaactgc ttacgcgcat tgcggaaaat gcgcatccat gggccatcct cgccccagtt
    12241 ttccggatgc caggagttgc tgacagtacg gaaaacacgt tccgggtgcg gcatcataat
    12301 ggtgactcga ccactttcag tcgtaaccgc cgtaataccg ttcggtgaac cgttcgggtt
    12361 agccgggtag gtttcagtga ctttgccgaa gttatcgaca tagcgcagtg ccaccagccc
    12421 tttgctttcc agtgccgcca gatgcgccgc atcacgcact tccacgcgcc cttcaccatg
    12481 agagacagca atcggcattt gcgagcccac catcccctgc aacagcagag acgggctttg
    12541 ggttacttca accaggctga aacgcgcttc aaagcgatcg gaggtgttac gcacaaaacg
    12601 tggccacaac tcgctccccg ggatcagttc acgcaggtta gacatcatct ggcaaccgtt
    12661 acataccccc agggccagcg tttgcggacg atggaagaag gttgcaaact catcgcgtac
    12721 acggtcattg aacaggatag acttcgccca accttcaccg gcacccagca catcaccgta
    12781 ggagaaacct ccgcacgcga ccagggcgtg aaaatcctcc agtcccgtac gtccagtcag
    12841 caggtcgctc atatgcacgt cgatagcatc aaagccccca cgttggaaag ctgccgccat
    12901 ttcaacatgc gagttcacgc cctgctcacg cagtacggca actttcggac gtgcgccagt
    12961 ggcaatatac ggtgctgcca catcttcgtt gatatcgaac gacagtttta cattcaggcc
    13021 cggatcggcg tcgttagatt tcgcctgatg ctcctgatcg gcacactccg ggttgtcacg
    13081 caggcgctgc atctgccagg tagtttctgc ccaccagaca cgcaacgtag tgcggctttc
    13141 gctgaataca gtctgcccgt tggcggtaat cacaaaacgg tcaccggaaa ccgcctgccc
    13201 aacataatgg acgcaatccg caagcccatg ctgtgccagt acggactcga ccgcttcacg
    13261 gtcagcggca cgaacctgaa tcaccgcacc cagttcttcg ttaaacaacg ccgccaggcg
    13321 atcgtcaccc agagaggcga tatccgcgtc aatgccacaa tgacctgcaa aggccatttc
    13381 cgccagcgtt accagcaggc cgccatcaga gcggtcgtga tacgccagca gcttacgctg
    13441 tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt tggacaacat cgcgtacatc
    13501 tgccggtttg tcgccaagct gacgataaac ctgcgccagc gccgttgcgc ccagcgcgtt
    13561 attgcctttg cccaaatcaa tcagcagcag tgcgttatct tcggtacaaa gctgcggcgt
    13621 gatggtgtga cgtacatctt ccacgcgggc aaatgcagaa atcaccagcg acagcggcga
    13681 cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt ttcatcgaca tagagtcttt
    13741 acccaccggg atcgtcaggc ccagcgccgg acaaagttct tcacccacgg ctttaacggc
    13801 ttcatacagg cccgcatctt caccagggtg gcctgccgcc gccatccagt tggcggaaag
    13861 tttaatgcgt ttgatatcgc caatctgtgt tgcggcaata ttggttaacg cttcaccgac
    13921 cgccagacgg gcagaggcgg cgaaatccag cagcgcaacc ggcgcacgct cgccaatcgc
    13981 catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg accgcgcagt tagcgaccgg
    14041 cacctgccac ggccccacca tctgatcgcg cgccaccatg ccggttacgc tgcggtcacc
    14101 gatggtcacc aggaaagttt tctccgccac ggtcggcaga tgcagtacgc gtttcaccgc
    14161 gtctgcaatg gtgatccctt cacggaccag cgcatcgccc ttcgctttca gcgtttgtac
    14221 atcgcgggtc atcttcggcg ttttaccaag caggacgtcc agcggcagat cgatcggctg
    14281 attatcaaaa tgacgatcgt gcagagaaag atgcagttct tcggtcgctt caccaatcac
    14341 cgcgtagggt gcgcgctcac gcttacacag ttcgtcaaac agcggtaatt gatcggcagc
    14401 aaccgccagc acgtagcgtt cctgggattc gttacaccag atttccagcg ggctcatgcc
    14461 cggttcgtcg cttagaatat cgcgcagttc aaatttaccg ccgcgtccgc cgtcgctcac
    14521 cagctccggc atcgcgttag aaagaccgcc cgcaccgacg tcgtggataa acagaattgg
    14581 gttagcatca ccaagctgcc agcaacggtc gatcacttcc tggcaacgac gctccatctc
    14641 cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca tcagactgac cagacgccat
    14701 ggaagacgcc gcgccgccgc caagaccgat gttcattgcc gggccgccga gaacgaccag
    14761 cttcgcaccg acgttgatct cgcctttttg tacgtgatcg gcgcgaatgt tgccgatccc
    14821 gcccgccagc atgatcggtt tgtgataacc gcgcagctct tcgccattgt ggctgttcac
    14881 tttttcttca taagtacgga agtagccgtt cagtgccgga cgaccaaatt cgttgttaaa
    14941 cgccgcaccg cccagcgggc cttcggtcat gatgtccagc gcggtaacaa tgcgctcagg
    15001 cttaccgaaa tcttcttccc acggctgttc gaagccagga attcgcaggt tggatacgga
    15061 gaaaccaacc aaaccagctt tcggcttcgc gccgcgcccg gtggcacctt catcgcggat
    15121 ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa atcgccgtcg ggtggttgtg
    15181 agtttcgact ttcatcagga tgtgcgccgg ttcctgatgg aaattgtagc ggcccgtttc
    15241 gtggtcagca aagtagcggc ccacttcaga accttccatt acggcggcgt tatctttata
    15301 agcagagaga acgtgatctg gcgtggtttc gaaagtattt ttgatcatcc tgaacagcga
    15361 tttcggctgt tgttcaccgt cgataatcca gtcggcgtta aaaattttgt ggcggcagtg
    15421 ctcagagttc gcctgagcga acatatacag ctcgatgtcg ttcgggttac gaccaagctt
    15481 tgtgaaagca tcctgcagat agtcaatttc atcttccgcc agagccaggc caagacgcag
    15541 gttagcgtcg atcagcgcct gacggccctg ccccagcaaa tcaacgcttg tgaccggagt
    15601 cggttgatgg tgggcaaaca actgctctgc atcatctaaa tcaaaaaaga ccgtttccat
    15661 catgcggtcg tgcagttcag cggtaacctg ctgccattgt tcattggtca gcgtaccggc
    15721 ttctatatag taagcaacgc cgcgctcaag gcggtttacc tgttgtagcc cgcagttatg
    15781 ggcaatatcg gtcgctttcg aagaccaggg agagatggtg ccaggacgcg gggtcaccag
    15841 caggagtttg ccttgcgggg cgtggctggc gagtgccgga ccatatttca gcaggcgttc
    15901 aagttgtgcg tgctcatcat cgtttaacgg cgcattgagg tcagcaaaat ggacatactc
    15961 ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa cgtgccagca gtttgttgat
    16021 tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc atcataagtc tctcgtcttc
    16081 taagctttcg gtgtacccaa gggggggaaa cgggcgtcat tataaagaat ctgatgcgct
    16141 gacgaaaccg tttgcgtgga aataaaatca ccatcgtgaa ttagcaacgc gtgccgccaa
    16201 tggctgtaat aagttgccat ctggcgcagg tttacgcaaa atgccgctca tttatgagta
    16261 aacctttcac tattattacg ttttttcaag ctgggacgcg cacgacacag agaattaact
    16321 aattgaaaaa attaaagatt aattatctgt tcatcggcat tctggcactg ctgctcgcgg
    16381 tcgctctctg gccatccatt ccctggtttg gtaaagccga caaccgtatc gccgccattc
    16441 aagcgcgggg agagttgcgt gtgagcacca ttcatactcc cctgacttat aacgaaatca
    16501 acgggaaacc ttttggcctg gattacgaac tggcgaaaca gtttgccgat tacctcggcg
    16561 taaaactgaa agtgaccgtg cggcagaata tcagccagct gtttgacgac cttgataatg
    16621 gtaacgccga cctgctggcg gcaggactgg tctataacag tgagcgggta aaaaattatc
    16681 agcctggccc tacctattat tccgtgtcac aacaactggt ttataaagtg ggtcagtatc
    16741 gcccacgtac gctgggcaac ctgacggcgg agcaactcac cgttgcaccg ggtcatgtgg
    16801 tggttaacga tctccagacc ctgaaagaaa caaaattccc ggaattaagc tggaaggtag
    16861 acgacaaaaa aggctctgcg gaattaatgg aagatgtcat cgaaggaaaa ctcgattaca
    16921 ccattgctga ttctgtcgcc atcagcctgt ttcagcgcgt tcacccggag ctcgccgtag
    16981 cgctcgatat caccgatgaa caaccggtga cttggtttag cccgttagat ggcgataata
    17041 ccctttccgc cgccctgctc gacttcttca acgaaatgaa tgaagacggt acgctggcac
    17101 gcattgaaga gaaatacctg gggcatggcg atgattttga ttacgtcgat acgcgcacat
    17161 ttttacgcgc cgtcgatgcg gtactgccgc agttaaagcc cctgtttgag aaatacgccg
    17221 aagaaattga ctggcgtttg ctggccgcta ttgcttatca ggaatcgcac tgggatgcac
    17281 aggccacttc accgacgggt gtgcgcggca tgatgatgtt aaccaaaaat accgcgcaaa
    17341 gcctcggcat tacggatcgt accgatgccg aacagagcat cagcggtggc gtgcgttatt
    17401 tgcaggatat gatgagtaaa gtgccggaaa gtgtgccgga gaacgaacgg atctggtttg
    17461 ccctcgctgc gtacaatatg ggctatgcgc atatgctgga tgcccgcgcc ctgacggcaa
    17521 aaaccaaagg gaatcctgac agttgggctg acgtaaaaca gcgtctgcct ttacttagcc
    17581 agaaacccta ttacagcaag ctgacttacg gctacgctcg tgggcatgaa gcctacgctt
    17641 atgtcgaaaa tattcgtaag tatcagatta gcctggtggg ttatctgcaa gagaaagaga
    17701 agcaggctac agaagcggcg atgcaactgg cgcaggatta tccggcggta tcgcctacgg
    17761 agttgggcaa agagaaattt ccttttctct cgtttctttc ccagtcgtca tcaaactatt
    17821 tgacccattc tccctctctg ctgttttcca ggaaagggag tgaagagaaa caaaattaat
    17881 ccgtcgagga ttgcgctttt ttctgcgctt taatttcctg gcggcgcatg cgaaagaagt
    17941 cactgagcaa cgccgcgcac tcatccgcca gtattccttc cgtaatttcc actcggtgat
    18001 tcatacccgg atgatgcagc acatccatta aagatcccgc agcgccagtt ttcgcgtcac
    18061 gcgcaccaaa gaccacgcga ccaatgcgac tgtggatcat cgctccggca cacattacac
    18121 atggttcaag cgtgacatac aacgtggcgt cgatcagacg ataattttgc atcaccagac
    18181 caccctgccg cagggccatg atttctgcat gtgcggtggg atcatggcga ccaatcgggc
    18241 ggttccagcc ttcgccgatt acccgattgt tatgcactaa taccgcgccg accggcactt
    18301 cccgctcatc ccaggcacgt ttcgccagcg tcagcgcgtg acgcatccag tattcgtggc
    18361 taaattcgac ttcagacaaa aagaaaactc cggttataaa agcgcggcgc attatacacg
    18421 gacgctatgc tttactctat tccagttgct ggagttcacc gcgcggggtt actcgccaac
    18481 gatgctgaca gaaataaagc agcggattgt cctgattact gtcgctatag ccactgtaca
    18541 gccgcagcgg cgtgccgatt ttgcgctcca gttgtgcgac cttttcatgt cccagacaac
    18601 gcatcgtcaa tacccaacca ccatagccac gctgaatttg gctggcgata agattaaccc
    18661 gcggcagcca gggcgtatcg aaataaaccg cttcaaccag cggctgcgga gagccggtaa
    18721 tcaaccagat atcagcatcg gaacttaaca ggtaggtggt taatcgctcc tgaaccagcg
    18781 gaaaggcggt aacattgtcg cgaaaccagc gcacgaaatc ggcctgcaac gtctgtaaac
    18841 gtgcttcgct gtgaccaaaa gtgcaccccc acagaagcag actcatcggc cagcgtgccg
    18901 cacgaccttt tatcaataac gcaatggcta taatcggtaa caacggcagg acaagtaacg
    18961 cattcagcgg ttggcgacgc agtaaatagc gcagaaaact gccgaacata tcctgctgat
    19021 gcaatgttcc atctaagtca aaaaacacca cacgacgctc gtgagttgcc aaaccatact
    19081 cctctggact gaaacatcct gattaatttt cgctactaca tagcctaaca gatagatcat
    19141 cacttttccg gcaacaatcg accgcatcat ggctaactgg aatttttaat tcatggcaat
    19201 tagcggcaat ggaatataaa attcactcgc gtgtgtctca tattgcctgt tgtcgccact
    19261 atttcatcgc gaggaaagat atgaacggct tacttcgtat ccgtcagcgt taccaggggc
    19321 ttgcccagag tgataaaaaa ctggcggatt atctgctgct acaacctgat acggcgcgcc
    19381 atttaagctc tcagcaactg gccaacgaag ccggagtcag tcagtccagc gtcgtgaagt
    19441 tcgcgcaaaa actcggctat aaaggttttc cggcgcttaa gttggcgttg agtgaagcgc
    19501 tggcaagcca gccggaatcg ccctccgtgc ccattcataa ccaaatccgc ggtgatgatc
    19561 cgttacggct ggtcggcgaa aaactgatta aagaaaatac tgccgctatg tacgcaacgc
    19621 taaacgttaa tagtgaagag aaactgcatg aatgcgtaac aatgttgcgc tctgcgcggc
    19681 ggataattct gaccggtatt ggcgcttcgg gtctggtggc gcaaaacttt gcctggaagc
    19741 tgatgaagat tggcttcaat gctgccgcac tgcgcgatat gcatgcgcta ctcgcaacag
    19801 tacaggcgtc gtcccctgac gatctgttat tagccatttc ctacaccggt gtacgacgcg
    19861 agttaaacct ggcggcagat gagatgctgc gagtgggcgg aaaagtgctg gcgattaccg
    19921 gctttactcc gaatgccctg caacagcgtg cttctcattg cctgtatacc attgccgaag
    19981 aacaagcgac aaacagtgct tcaatctctg cttgtcacgc tcagggaatg ttaacggatt
    20041 tgctgttcat tgcgctgatt cagcaggatc tggaactggc accagaacgt attcgtcata
    20101 gtgaagcgct ggtgaaaaaa ctggtctgag taaagaatgc gcgtataatg cccgcccggt
    20161 ttgtgttgtt ttgagagttt ccttatggcg ctgttaatca ctaaaaaatg catcaattgt
    20221 gatatgtgtg aaatcaaata aaaacacatt tattttcata gtgatacagc aatgtgttat
    20281 tgaatgtgtt gttggattta tggtgcaaaa aaattttgca taagttcagc aacacaaaga
    20341 acaccacttt ctttaacgtc ctccgcgccc taaaggacgg ggctttacgg cgcaaaaggc
    20401 ctgtgacata caacattaac ttttcagtca accgtagcac gtccttgtat acgccgtgtc
    20461 tgcggtttta tataaccctg gctggaaacc tcttatacaa agtcgattcg ccaacatcat
    20521 agatgatcgc caccttctgg cgagaaactc ctgatgcaat tagtcgcccg gcctgcgccc
    20581 attattctgg tgaaagtttg ggacggcgac cgccaattcg tccctgtgcg cgagcagctt
    20641 ccagtcctgc ttttgttcgt tcaacaatca gttcacgttc catttcagcc agggcaccca
    20701 tcacatggaa gaaaaagcgt cccattggtg tgctggtatc aattgaatca gtcagactac
    20761 gaaagttgat gcctcgttcg cgcaactcct ccaccagcac gacaagatgc cgcatactgc
    20821 gccccagccg atccagcttc cagacaacca gtgtgtcgcc tgccgataat gtcctgagca
    20881 gttttttcag ccccggtctt tcggactttg taccacttat cttgtcttca aaaatcagct
    20941 cgcatcctgc acagttcaac gcattacgtt gtagatcggt attctggtca tttgttgata
    21001 cgcgtacata gccaataagc atggtaaatc cccctgtta
//
LOCUS       DADLTF010000068        20741 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.068, whole genome shotgun sequence.
ACCESSION   DADLTF010000068 DADLTF010000000
VERSION     DADLTF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.068"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="JN749_002278"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="JN749_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HAY7368675.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="JN749_002279"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="JN749_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HAY7368676.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="JN749_002280"
     CDS             complement(977..1267)
                     /locus_tag="JN749_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HAY7368677.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="JN749_002281"
     CDS             1701..2411
                     /locus_tag="JN749_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HAY7368678.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JN749_002282"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="JN749_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HAY7368679.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="JN749_002283"
     CDS             complement(3539..4198)
                     /locus_tag="JN749_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HAY7368680.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGVLSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /locus_tag="JN749_002284"
     CDS             4351..6684
                     /locus_tag="JN749_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7368681.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="JN749_002285"
     CDS             complement(6653..7093)
                     /locus_tag="JN749_002285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7368682.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JN749_002286"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="JN749_002286"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAY7368683.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="JN749_002287"
     CDS             7811..8509
                     /locus_tag="JN749_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7368684.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="JN749_002288"
     CDS             8738..9946
                     /locus_tag="JN749_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368685.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="JN749_002289"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="JN749_002289"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HAY7368686.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASAMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="JN749_002290"
     tRNA            12053..12129
                     /locus_tag="JN749_002290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="JN749_002291"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="JN749_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HAY7368687.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FESVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="JN749_002292"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="JN749_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HAY7368688.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="JN749_002293"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="JN749_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HAY7368689.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="JN749_002294"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="JN749_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HAY7368690.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="JN749_002295"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="JN749_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HAY7368691.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="JN749_002296"
     CDS             complement(18744..20015)
                     /locus_tag="JN749_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HAY7368692.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20491..20598
                     /locus_tag="JN749_002297"
     CDS             20491..20598
                     /locus_tag="JN749_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HAY7368693.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4964 a         5131 c         5465 g         5181 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatagcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcacac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgtct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgcc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cctggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggcctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aatcggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcggcaagcc ggaagggttc cggtgaggcg caatgttgcg
    20341 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20401 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20461 aaacgttgtc attacaacac aggcggctat atgacgctcg cagagctggg catggccttc
    20521 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    20581 ctgaacaagc ggaagtagcg tgtcatgtgg gcgtcaggct gccgtaatgg caatttgcgc
    20641 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    20701 gcaagcggat aatattctgc ggaatgcgca gcacagagtg a
//
LOCUS       DADLTF010000069        20548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.069, whole genome shotgun sequence.
ACCESSION   DADLTF010000069 DADLTF010000000
VERSION     DADLTF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.069"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="JN749_002298"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="JN749_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAY7368694.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="JN749_002299"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="JN749_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HAY7368695.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="JN749_002300"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="JN749_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HAY7368696.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN749_002301"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="JN749_002301"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HAY7368697.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFCKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN749_002302"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="JN749_002302"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HAY7368698.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4448..4813
                     /locus_tag="JN749_002303"
     CDS             4448..4813
                     /locus_tag="JN749_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HAY7368699.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4963..5481
                     /locus_tag="JN749_002304"
     CDS             4963..5481
                     /locus_tag="JN749_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4154 domain-containing protein"
                     /protein_id="HAY7368700.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5471..6696
                     /gene="dgcN"
                     /locus_tag="JN749_002305"
                     /pseudo
     CDS             5471..6696
                     /gene="dgcN"
                     /locus_tag="JN749_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6712..7194
                     /locus_tag="JN749_002306"
     CDS             6712..7194
                     /locus_tag="JN749_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HAY7368701.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQLQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="JN749_002307"
     CDS             complement(7271..7618)
                     /gene="rplS"
                     /locus_tag="JN749_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAY7368702.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="JN749_002308"
     CDS             complement(7660..8427)
                     /gene="trmD"
                     /locus_tag="JN749_002308"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAY7368703.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="JN749_002309"
     CDS             complement(8458..9006)
                     /gene="rimM"
                     /locus_tag="JN749_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAY7368704.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="JN749_002310"
     CDS             complement(9025..9273)
                     /gene="rpsP"
                     /locus_tag="JN749_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAY7368705.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="JN749_002311"
     CDS             complement(9410..10771)
                     /gene="ffh"
                     /locus_tag="JN749_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAY7368706.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10863..11729
                     /locus_tag="JN749_002312"
     CDS             10863..11729
                     /locus_tag="JN749_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HAY7368707.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLVIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIVQQLIS"
     gene            11750..13036
                     /locus_tag="JN749_002313"
     CDS             11750..13036
                     /locus_tag="JN749_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7368708.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="JN749_002314"
     CDS             complement(13091..13684)
                     /gene="grpE"
                     /locus_tag="JN749_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAY7368709.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13807..14685
                     /gene="nadK"
                     /locus_tag="JN749_002315"
     CDS             13807..14685
                     /gene="nadK"
                     /locus_tag="JN749_002315"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HAY7368710.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14771..16432
                     /gene="recN"
                     /locus_tag="JN749_002316"
     CDS             14771..16432
                     /gene="recN"
                     /locus_tag="JN749_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAY7368711.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16581..16922
                     /gene="bamE"
                     /locus_tag="JN749_002317"
     CDS             16581..16922
                     /gene="bamE"
                     /locus_tag="JN749_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HAY7368712.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16984..17274)
                     /locus_tag="JN749_002318"
     CDS             complement(16984..17274)
                     /locus_tag="JN749_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HAY7368713.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17264..17740)
                     /gene="ratA"
                     /locus_tag="JN749_002319"
     CDS             complement(17264..17740)
                     /gene="ratA"
                     /locus_tag="JN749_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HAY7368714.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGG
                     WKFTPLSQEACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17872..18354
                     /gene="smpB"
                     /locus_tag="JN749_002320"
     CDS             17872..18354
                     /gene="smpB"
                     /locus_tag="JN749_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAY7368715.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18569..18931
                     /gene="ssrA"
                     /locus_tag="JN749_002321"
     tmRNA           18569..18931
                     /gene="ssrA"
                     /locus_tag="JN749_002321"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19136..20377
                     /locus_tag="JN749_002322"
     CDS             19136..20377
                     /locus_tag="JN749_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7368716.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIEPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5304 a         5368 c         4823 g         5053 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac catcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcag aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccaa gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat atataaattt acaccccttt gaaaacaatc cgctatgctt tgaaaaagga
     4441 gaaagaaatg atgaaaaagt ttatcgcccc cttgttggct ttactggtta gcggatgtca
     4501 gattgatcct tatactcacg cgccaacctt gaccagcacc gactggtatg atgtcggtat
     4561 ggaagatgcg atatcggaca gcgccataaa agatgacgat gcatttagcg attcacaggc
     4621 ggatcgcggt ctatacctta aaggatatgc cgaagggcaa aagaaaactt gccagaccga
     4681 ttttacttat gcccggggac tttccggtaa aagctttcct gccagctgca acaatgttga
     4741 aaatgccagc caactgcatg aagtatggca aaaaggggct gatgaaaatg ccagcacaat
     4801 acgattaaat taattaaaac cccacaaata aattaaacat aagattttac tcattgtgtt
     4861 ttatttctca cattgatgac ggtcgcatga aaaaatgata ttttaatgag catcctgaaa
     4921 aaatatcgat gcatttcgag cgaagatggt ggggatccct gaatgcgttt ttctcaccga
     4981 ctgttcttac ttctgattct cctgctgacg ggagcaccaa tactcgctca agagccttct
     5041 gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac ccgctggcct
     5101 gcgctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag taccgcccta
     5161 caagagaatg ccgcgacatc tttgccttat ctgcctgtca tcattcacac tcaacaagag
     5221 gcgatgattt caggctgcaa tggtttttat tttggcaacg agtcaccgac attccagatg
     5281 gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca aaataccgag
     5341 tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag atttgccgta
     5401 aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt aatgctcgca
     5461 cggaagaaaa atgatggata acgataattc tcttaataag cgccccacgt ttaaaagagc
     5521 attacgcaac atcagtatga ccagcatatt tatcactatg atgttgatct ggttgctgct
     5581 ttccgtgacc tcggtgctga ccctgaaaca gtacgcgcaa aaaaacctgg cactgacagc
     5641 agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc ctgcagcaac
     5701 tgaaacactg gcagcgctgg gccagcaagg gcattttcaa ctgcagaagt acgtgataag
     5761 cagcaaaata ttctggcatc ctggcattac ccccgtaagg atccaggcga tactttcagc
     5821 aatttcataa gccactggct cttccctgcc cccatcattc agccgattcg tcacaatggt
     5881 gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt caattagcca ttttatctgg
     5941 ttttcactcg ccgtactgac cggttgtatt ctgctggcat caggcatcgc aattaccctc
     6001 acccgccatt tgcacaatgg cctggtggaa gcactgaaaa atatcaccga tgtcgtacat
     6061 gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg aagaacgtat cgctgagttt
     6121 caccgcttcg ctctcgactt caacagtctg ctggatgaaa tggaagagtg gcagcttcgt
     6181 ttacaggcca aaaatgcgca gcttctgcgt accgcgctac atgacccatt aaccgggctg
     6241 gctaaccgcg cagcgtttcg tagcggcatc aatacgttga tgaacaattc cgatgcccga
     6301 aaaacgtcgg cgttactatt tcttgatggc gataatttca aatatatcaa tgatacctgg
     6361 ggtcatgcga cgggcgatag ggtcttgatt gaaatcgcaa aacggttagc tgaatttggc
     6421 gggctgcgac ataaagcata ccgcctgggc ggcgatgaat tcgctatggt gctctatgat
     6481 gtacagtcgg aatctgaagt gcagcagata tgctcagcac tgacacaaat ctttaatctc
     6541 ccgtttgatc ttcataatgg ccatcagacc accatgacat taagcattgg ttacgcgatg
     6601 accattgagc acgcctctgc ggaaaaatta caagagcttg ccgatcacaa tatgtatcag
     6661 gccaaacacc agcgtgccga aaagctggtg agataacaag gatatatcga tatgataaag
     6721 cacctggtag cacccctgat tttcacctca ctaatactga ctggctgcca gtcccctcag
     6781 ggaaagttta ctcctgagca agtcgccgct atgcaatctt atggatttac tgaatccgcc
     6841 ggcgactggt cgctgggctt atcagatgcc attctgttcg caaaaaatga ctacaaattg
     6901 ctcccggaaa gccagcaaca gctccaaacc atggcagcca aattggcctc gacagggcta
     6961 acacatgccc gtatggatgg acacaccgat aactatggtg aagacagtta caacgaaggc
     7021 ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg ctattggtgg acaaattcca
     7081 cgcagcaatc tcaccacaca gggtttagga aaaaaatatc ccatagccag taacaagacc
     7141 gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga ttacaacccc ttaaaacacg
     7201 agcaaacaga caaaaaaagg gccagccaat tgcccagccc ttcttaacag gatgtcgctt
     7261 aagcgaaatc ttagttaaga cgctctttga tacgagcagc cttaccagta cgctcacgca
     7321 ggtagtacag tttagcttta cgaacagcac cacgacgttt gacagaaatg ctgtcaacta
     7381 ccggagagtg agtctggaag acacgctcaa cgccttcgcc gttggaaatt ttacgaacag
     7441 tgaatgcaga gtgcagaccg cggttacgaa tagcgataac cacgccctcg aatgcctgca
     7501 gacgtttttt ggaaccttca acaacccata ctttcacttc cacggtatca cccggacgga
     7561 aggaaggtac gtcctgcttc atctgctctt gttcaagttg cttaataatg ttgctcataa
     7621 tttaatctct tatcctgggt aaactgatct ctcgggggct tacgccatcc catcatgttt
     7681 atgttgctgt tgtgcgtgtt ccgttttgaa ctccgccagc aaccttgctt gctcttcagt
     7741 cagagccagg ttttccagaa gttcaggtct tctaagccag gtacggccca gcgactgttt
     7801 caaacgccag cgacgtatct cggcatggtt gcccgacagt aacactggcg gaacttccat
     7861 cccttctaac acctcaggcc gcgtatagtg cgggcaatcc agcaatcctt cagcaaagga
     7921 atcttccgtt gccgaggctt catgtcccag tactcccgga ataaaccggg aaacggagtc
     7981 aatcagcgtc attgctggta actcaccacc actgagaacg taatcgccga ttgaccattc
     8041 ttcgtcaatt tcggtttgga tcacgcgctc atctatacct tcgtagcgac cgcacaccag
     8101 aatcaatttt tgattcgttg ccagttcgct gacgcccgct tgatcaagct tgcgtccctg
     8161 tggtgacaga taaatcacct ttgcgccttc acccgccgcg gcttttgctg catgaatggc
     8221 gtcccgcaag ggttgcacca tcattaacat ccccggtccg ccgccgtaag gacgatcgtc
     8281 cacggtacgg tgccggtcat gcgtgaagtc gcgaggactc caactctgga tgctcagcag
     8341 gccattttta actgcccggc cagttacccc gtaatcggta attgcgcgga acatttcagg
     8401 aaacaggcta attatgccaa tccacatagc gccgtctttt accgtttatc cggtggttta
     8461 aaaaccagga tcccaatcta cttcgattga acgagtagtg agatcgactt tcttgataac
     8521 ctgcccatcg aggaacggta cgagacgttc cttgatacca aacgcatctt tcaggtttgc
     8581 cttaatgacg agaacgtcat tagatccggt ttccatcata tcgacgactt taccgagatc
     8641 gtagccttca gtggttacta cctggcagcc catcaggtct ttccagtagt agtccccctc
     8701 ttcaagctga ggcagctgcg atgaatccac gacaatttca caattcgtca gcaggttcgc
     8761 cgcatcacga tcgtcaacgc ctttcagctt gatgatcatg tcctgattgt ggtgcttcca
     8821 gctttccagc tggacttgct gccactgacc cgccttctgg ataaaccagg gctgatagtc
     8881 aaaaatgctt tcggcgtctt cggtggaaga aaacactctg agccacccac gaataccgta
     8941 agacgaaccc atttttccca aaacgatggg atcaacaggt gcttgcgcgg tgagttgttt
     9001 gctcatcatg accaccgtga cagattaagc tgctttgttt acttctttga tcagcgcagc
     9061 aacgcgatca gaaatagttg cgccctggcc aacccagtga gcgatgcgat ccagatccag
     9121 gcgagtgcct tcttcttttt cgctagcgat tgggttgaag aaaccaacgc gctcgatgaa
     9181 gcgaccgttg cgtgcattac ggctgtcagc gacaacaacc tggtagaacg gacgcttttt
     9241 agcgccgtga cgtgctaaac gaatagttac cataacatcc tcttgtgtga ataaaacaac
     9301 cggaccccat cgaggaacgg agtccggtgt catattaaaa gcccgaaaat tttactgatt
     9361 tctggagaaa atgcaatcag cagttaataa ctctgtcgca taaggtcatt tagcgaccag
     9421 gaaatcccgg cggcatcatt cccttcatgc ttctcatcat cttcgccatt ccgcctttct
     9481 tcattttctt catcatgcgc tgcatgtcgt cgaactgctt cagaaggcgg ttaacgtcct
     9541 gtacctgcat cccgcaaccg gcggcgatac gacgcttacg cgaacctttg atgatttctg
     9601 gcttagcgcg ctctttcatc gtcattgagt tgatgatggc ttccatacgc accagcactt
     9661 tatcgtccat ctgcgacttg acgttatccg ggatctgccc catgcccggc agtttgccca
     9721 tcaagctggc catgccgccc atatttttca tctggcgcag ttgctcaaga aagtcgttga
     9781 gatcgaagcc gtcgcctttt tttagcttgc tggctaattt ctctgcctgc gcgcggtcaa
     9841 ctttgctttc gatatcttcg atcagcgaca gtacgtcgcc catgccgaga atacgcgacg
     9901 cgatgcggtc cggatggaac ggctccagcg cctcagtttt ctcgccaaca ccgaggaact
     9961 tgatcggttt gccagtgatg tgacgaatag agagcgccgc accgccgcgg gcatcgccgt
    10021 ccactttggt caacactacg ccggtaagcg gtaacgcttc attgaatgct tttgccgtat
    10081 tggccgcatc ctgaccggtc atggcgtcaa ccacaaacag ggtttcaacc gggttaatcg
    10141 acgcatggac ttgtttgatc tcgtccatca tcgcttcgtc aacgtgcaga cgaccagcgg
    10201 tatccaccag cagcacgtcg tagaatttca gtttggcttc tttcagcgcc gcgttaacaa
    10261 tatcaaccgg cttctgacca acatcagaag ggaagaaatc aacgcccacc tgctctgcca
    10321 gcgtctcaag ctgtttgatt gccgccgggc gatagacgtc agcagaaacc accagcactt
    10381 tcttcttgtg cttctcgcgc aggaaattac cgagtttacc aacgctggtt gttttaccgg
    10441 caccttgcag gcctgccatc agcacgaccg caggcggttg cgcagccaga ttcagggtct
    10501 ggttctcttc gcccatcgcc gcaaccagtt cgttacggac gattttgacg aactcctgcc
    10561 ccggcgtcag gctcttatta acttcatgac caaccgcttt ctcttttacg cgattgataa
    10621 actcacgcac taccggcaga gcgacgtccg cctccagcag cgccatgcgc acttcgcgca
    10681 gcgtatcttt tacgttgtct tcagtgaggc gtccacggcc actgatattg cgcagcgtgc
    10741 gcgacaaacg atcggttaaa ttatcaaaca ttgtctctcg cctggggtgg aaacggttgg
    10801 ccgcaatcgc gacacatcat cagtattttg ccgcagtata acatgaaggc gtctttgttg
    10861 ttatgcaacg gttggagcag cgttcacctg acgctatact gcttctcttt cttattgctc
    10921 aaactgtcga cattactatg cccgtttttg ctctgctcgc gcttgtcgcc tactccgtca
    10981 gtcttgcgct gattgttccc ggtctgctgc aaaaaaacgg cggctggcgg cgcatggcta
    11041 ttatttctgc ggtcattgcg ctggtctgcc acgcaatcgc tctggaagcc cgcatcctgc
    11101 ccgacggtga tagcggacaa aacctcagcc tgctgaacgt tggttcattg gtcagtttgg
    11161 tgatctgtac ggtaatgacc attgtggctt ctcgcaatcg tggctggttg ctgctaccca
    11221 ttgtctatgc ctttgcgctt atcaacctgg cgctggcgac cttcatgccc aatgaataca
    11281 tcacccatct ggaagcaacg cctgggatgc tggtgcacat tggcttatcg ctcttttcct
    11341 atgccacgct aattatcgcc gccctgtacg cgctgcaact ggcgtggatt gattaccaac
    11401 tgaagaacaa gaagctggca tttaaccagg aaatgccgcc attgatgagt atcgagcgta
    11461 aaatgttcca catcacgcag attggcgtgg tgctgctaac gctcacgctt tgcactggcc
    11521 tgttctacat gcacaaccta tttagcatgg aaaatatcga caaggctgtg ctctctatcg
    11581 tggcgtggtt tgtctatatt gtgctgctgt ggggacatta ccatgaagga tggcgtggac
    11641 gccgcgtcgt ctggtttaac gttgcgggcg cggtaattct gacactggcc tacttcggca
    11701 gccgaattgt ccagcagtta atcagctaaa cccagaaaag gagtttcccc tggaacacat
    11761 ttctactact acgttgatca ttattctgat catcatggtg gtcatttcag cctatttttc
    11821 cgggtccgaa accggaatga tgaccctcaa ccgctatcgt cttcgacata tggcgaaaca
    11881 gggtaatcgc tcggccaaac gcgtcgaaaa attgctgcgt aagccagacc gcctgataag
    11941 cctggtgtta atcggcaata acctggtcaa tattcttgcc tccgcgctcg gcactattgt
    12001 cgggatgcgt ttgtacggcg atgcgggcgt ggcaattgcg actggtgtgc tgacttttgt
    12061 cgtactggtt tttgctgagg tattgccgaa aaccattgcc gcgctgtacc cggaaaaagt
    12121 cgcttatccg agtagttttc tgctggctcc gctgcaaatt ttgatgatgc cgctggtctg
    12181 gttgctgaat gctatcaccc gtatgctgat gcgcatgatg ggtatcaaaa ccgatatcgt
    12241 ggttagcggc tctttgagca aagaagagtt gcgcactatc gtgcatgaat cgcgctcaca
    12301 aatttcccgt cgcaatcagg atatgctgct gtcggtgctc gatctggaaa aaatgaccgt
    12361 tgatgacatc atggtgccgc gcagtgaaat tatcggtatt gatatcaacg atgactggaa
    12421 atctattctg cgccaactct cccactcacc tcacgggcgc atcgtgcttt accgtgattc
    12481 gctggacgac gccatcagta tgctgcgagt acgtgaagcc tggcggttga tgtcggagaa
    12541 aaaagagttc accaaagaaa ccatgctgcg cgccgcggac gagatctatt ttgtgcctga
    12601 aggtacgccg ctcagcacgc agttggtaaa gtttcagcgc aacaaaaaga aagtcggcct
    12661 ggtcgtcaac gagtatggag acattcaggg gctggtgacg gttgaagata ttctggaaga
    12721 gattgtcggc gatttcacta cgtcgatgtc gccaacactt gccgaagagg tcacgccgca
    12781 aaacgacggt tcggtgatta tcgatggcac cgccaacgtg cgggaaatca acaaagcctt
    12841 taactggcat ctaccggaag atgatgcccg cacggttaat ggcgtcattc ttgaggcact
    12901 ggaagagatc cctgtcgcag gcacccgcgt gcgtattggc gagtacgata tcgatattct
    12961 cgacgtacag gacaatatga ttaagcaggt aaaagttttt cctgtgaaac cgctgcgcga
    13021 gagtgtggcg gagtaacgaa aacggccctg cattcgcatg cagggccgtg aattattacg
    13081 aaaacagaaa ttacgctttt gctttcgcta cagttaccat cgccgcacga atcgtacgac
    13141 cattcagcgt ataacccttc tgcataatgc ccagtacgtt acctggcgca acgtcatcag
    13201 attccaccat tgcgatagcc tgatgcacat tcgggtccag cggcacgtta gtttcggcga
    13261 tcacttcaac accaaactta cgcacaacat ccagcatcga cttcagcgtc agctcaatgc
    13321 cttcaaccat cgcagacata tccgggttag ctttatcagc cacttccagc gcacgatcca
    13381 ggctatcaat caccggcagc aattcgttga tgaatttctc cagcgcgaat ttgtgggctt
    13441 tttcaatatc cagttcagta cgacgacgca ggttttccat ttcggctttt acgcgcaaaa
    13501 taccgtcacg ttcacgggtc tgggcttcag ccagctgagc ttcgagattc gcaatttttt
    13561 catcgcgcgg atccacctgc tcagcagaag cttctggctc aactgcctca atctcttcgt
    13621 gctgatccat gataatttct tccggggctt gcccctcagg cgttttctgt tctttactac
    13681 tcatgaattt ctccgcgttt ttttcgcatt catctcgcta acttcgctta ttatggggat
    13741 cagtttcagg gtttcaaggg aagcactcac attgtcatca atcttcgcaa caaggacctc
    13801 ggaaaaatga ataatcattt caagtgcatt ggcattgtgg gacacccacg gcaccctact
    13861 gcactgacaa cacatgaaat gctctaccgc tggctgtgca caaaaggtta cgaggtcatc
    13921 gttgagcaac aaatcgctca cgaactgcaa ctgaagaatg tgaaaactgg cacgctcgcg
    13981 gagattgggc aactggctga tctcgcggta gtcgttggtg gcgacggtaa tatgctgggc
    14041 gcggcgcgca cgctcgcccg ttacgatatt aaagttattg gaatcaaccg tggcaacctg
    14101 ggtttcctga ctgaccttga ccccgataac gcccagcaac aattagccga tgtgctggaa
    14161 ggtcactaca tcagcgagaa acgttttttg ctggaagcgc aagtctgtca gcaagattgc
    14221 caaaaacgca tcagcaccgc gattaacgaa gtggtacttc atcctggcaa agtggcgcat
    14281 atgatagagt tcgaagtgta tatcgacgag atctttgcgt tttctcagcg atctgatggg
    14341 ctaattattt cgacgccaac aggctccacc gcctattccc tctctgcggg cggtcctatt
    14401 ctgaccccct ctctggatgc gattaccctg gtgcccatgt tcccgcatac gttgtcagca
    14461 cgaccactgg tcataaacag cagcagcacg atccgtctgc gtttttcgca tcgccgtaac
    14521 gacctggaaa tcagttgcga cagccagata gcactgccga ttcaggaagg tgaagatgtc
    14581 ctgattcgtc gctgtgatta ccatctgaat ctgattcatc cgaaagatta cagttatttc
    14641 aatacattaa gcaccaagct cggctggtca aaaaaattat tctaatttta cgccagcctc
    14701 tttactgtat ataaaaccag tttatactgt acacaataac agtaatggtt tttcatacag
    14761 gaaaacgact atgttggcac aactgaccat cagcaacttt gctatcgttc gtgagcttga
    14821 gattgatttt catagcggca tgaccgtaat aactggcgag accggtgcgg gtaaatctat
    14881 tgcaatagat gccctcggtc tttgtctcgg tggtcgcgct gaagccgaca tggtgcgtac
    14941 cggcgctgct cgcgctgacc tgtgcgcccg tttttctctg aaagatacgc cagcggccct
    15001 gcgctggctg gaagaaaacc agcttgaaga cgggcatgaa tgtttgcttc gtcgcgtgat
    15061 cagcagtgat ggtcgctccc gtggtttcat caacggtaca gctgttcctc tgtcacaact
    15121 gcgcgaactg ggtcagttgc tgattcagat ccatggtcag cacgctcatc aattactcac
    15181 caaacctgag caccaaaaat tcctgcttga tggctatgcc aatgaaacct ctctacttca
    15241 ggaaatgacc gcacgttatc agttgtggca tcaaagctgc cgtgacctcg cgcatcatca
    15301 acagttaagt caggaacgcg ccgcccgtgc ggaactgctg caataccaat taaaagaact
    15361 taacgaattt aatccgcagc ccggagagtt tgagcaaatc gacgaagagt acaaacgtct
    15421 ggcgaacagc ggtcaattgc tgaccaccag ccagaatgca ttggcattaa tggccgacgg
    15481 tgaagacgca aacctgcaaa gtcagcttta cacggctaaa caactggtga gcgaattgat
    15541 tggcatggac agcaaactgt ccggcgtact tgatatgctg gaagaagcta ccatccagat
    15601 tgctgaagcc agcgacgaac tgcgccacta ctgcgatcgt ctggatctcg atcccaaccg
    15661 actgtttgaa cttgaacagc gcatctcaaa acagatttcg ctggcacgta aacatcacgt
    15721 cagccctgag gcattaccac agtattacca gtcgctactg gaagaacagc agcaactgga
    15781 cgatcaggcc gactcacaag aaacgctcgc gctggcggta acgaaacatc atcagcaggc
    15841 actggaaacc gcgcgcgcat tacaccaaca acgccagcaa tatgcagaag aacttgcaca
    15901 gctgatcacc gacagtatgc atgcgctctc aatgccgcat gggcagttta cgattgatgt
    15961 caaatatgac gagcatcacc tgggcgctga cggtgccgat cgtattgagt ttcgggtaac
    16021 caccaaccca ggtcagccaa tgcagcctat tgccaaagtc gcatccggtg gtgaattgtc
    16081 ccgcatcgca ctggcaatcc aggtcatcac ggcgcgtaaa atggaaaccc cggcactgat
    16141 ttttgatgaa gtggatgtag ggattagcgg tccaacagcg gcagttgtcg gcaaactgct
    16201 gcgtcaactc ggcgaatcaa ctcaggtgat gtgtgttacc cacctgccac aagtcgcggg
    16261 atgtggtcat caacactatt ttgtcagcaa agaaaccgat ggtgcgatga cagaaacaca
    16321 tatgcaatct ctggataaaa aagcgcggtt acaagagctg gcgcgcctgc ttggtggcag
    16381 tgaagtcaca cgtaacacac tggcgaatgc gaaagaactg cttgcagcgt aaactttttt
    16441 cctgcttcac ggtcagagta aacagcaaaa cgccgtaaga ccggaaagca aaaggtttta
    16501 aagtgatgaa aggtctatta tcatcggcat attacagatg agccacgtac tgctcgggcc
    16561 cgaaaaggaa tcaaatcact atgcgctgta aaacgctgac tgctgcagca gcagtactat
    16621 tgatgttgac cgcaggctgt tccactctgg agcgagtggt ttaccgtcct gacatcaacc
    16681 aggggaacta tctgaccgct aacgacgtat ccaaaatacg tgttggcatg acgcaacaac
    16741 aagttgcgta cgcattgggt acaccgctga tgtccgatcc atttggtacg aatacctggt
    16801 tctatgtctt ccgccagcaa ccaggtcatg aaggtgtaac gcagcaaacg ctgacgctga
    16861 cctttaacag tagcggtgtg ttgaccaata ttgataacaa acctgcgctg agtggtaact
    16921 aataataaag ttgctctcaa agacgttaaa aaaggtgctc aatgagcacc ttttttctgt
    16981 ctgttattta ttcgctgatt tttctgctcg ttgcctgcga agctctttcg gatcggcaat
    17041 gagagggcga taaatctcca cccgatcgcc atcatgcaca ctatcgctta gttttgccgg
    17101 acggctgtaa atgccgactt tgtttttagt taaatcgata tcggtacgca attccagcaa
    17161 gccactggcg cgaatagctt cttcaaccgt cgcgccctcc tgcagcgtca ctcgctgcag
    17221 gtactgcttc tcaggtagcg cataagccac ctcaacggca attttaccta gcactgtaaa
    17281 cctctttcgc acgaaccgta aaagcctgga ccatattagc cgccagctct ttaaacacgc
    17341 gaccaaaggc gagttcaatc aacttattgg taaactcaaa gtcgagatga aactcgatac
    17401 gacacgcctc ctggctcagc ggcgtaaact tccatccacc aatcaatttc ttgaacggcc
    17461 catccaccag attcataaga atactttggt tactggtcaa ctggttgcgg gtagtaaacg
    17521 ttttgctgat cccagcctta gagacatcta ccgccgcagt catctgccca ggagtggact
    17581 ccagaatccg acttccggta caacctggca aaaactgagg ataagactga acgtcattca
    17641 ctaactgata catttgctcc gcgctgtagg gtaccagtgc ggtccggcta atctgaggca
    17701 taacaatttc catcaacaaa aatccaacaa ataatatcat ttatcctgct gtaaaaaaaa
    17761 cgctatcccg gcgctgggta acatcgggtt catgctaaga tagagccttg tcccccgcag
    17821 gattgatatg gggtgttttc gatttcagat taccgatgat tcacgacgct tatgacgaag
    17881 aaaaaagcac ataaacctgg ttcagcgacc atcgcgctta acaagcgcgc ccgtcacgaa
    17941 tactttatcg aagaagagtt cgaagcggga cttgccctgc aaggctggga agttaaatcc
    18001 ctgcgcgcag gaaaagccaa tatcagcgac agctacgtcc ttctgcgtga cggagaggca
    18061 tttctgtttg gcgctaacat cacgccaatg gccgtggcct ccacgcatgt ggtgtgcgat
    18121 cctacccgta cccgcaagtt acttctcaac cagcgcgaac tggactcatt gtacggtcgc
    18181 gtcaatcgag aaggctatac cgtagtggcg ctctccctgt actggaaaaa tgcctggtgc
    18241 aaagtgaaaa tcggcgtcgc caaaggtaaa aaacagcacg ataaacgttc agatatcaaa
    18301 gaacgcgaat ggcaggtgga taaagcacgc atcatgaaaa acgcccaccg ttaaacctgc
    18361 actccaatta ttgaccagtt cctcaccgcg cctccctctc cggcggcgcg aatgaacatc
    18421 ttattggcta tcacatccga cacaaatgtt gccatcccat tgcttaatcg aataaaaatc
    18481 aggctacatg ggtgctaaat ctttaacgat aacgccattg aggctggtca tggcgctcat
    18541 aaatctggta tacttacctt tacacattgg ggctgattct ggattcgacg ggatttgcga
    18601 aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa aatagtcgca
    18661 aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct ctccctagcc
    18721 tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg tggaagccct
    18781 gcctggggtt gaagcgttaa aacttaatca ggctagtttg ttagtggcgt gtccgtccgc
    18841 agctggcaag cgaatgtaaa gactgactaa gcatgtagta ccgaggatgt aggaatttcg
    18901 gacgcgggtt caactcccgc cagctccacc aaaattctcc atcggtgatt accagagtca
    18961 tccgatgaag tcctaagagc ccgcacggcg caagccctgc gggctttttt gtgccccgaa
    19021 tttgtcccgc gaagtctgat gccaactaat taaatccgat ccttttaggc accttgttag
    19081 gcacttcata aagctatatt gtctttgagg tgcctaaaac tatggaaacc cggcaatggc
    19141 aagacaaacc aaacctctat ccgttaagga aatcgaatct gctaaaccca aggaagcgga
    19201 ctacgttctc tatgatggcg atggccttga gctactcatt aaatccagcg ggagtaaaat
    19261 ctggcagttt cgctacattc gccctgtcac caagacacga gcaaagaaaa gcataggccc
    19321 ttacccgtca gttacccttg ccgatgccag aaactaccgt gcagagtccc gctcactcct
    19381 agctaaacag attgagccgc aggaacatca gcaagaacaa ttgcgtagtt cgctggaagc
    19441 caaaaccaat actttccagc tcgtagctga acgttggtgg aatgtgaaga aagccagtgt
    19501 gacagaggac tatgcggaag atatctggcg ctctcttgaa agagatgtct ttcctgcgat
    19561 tggcgacgtt agcgttacag atattaaagc tcatacactg gttcaggccg tacaaccggt
    19621 tcaggccaga ggagcactgg aaacggttcg tcgccagtgt caacgtatta atgaggtcat
    19681 gatctatgcc cagaacacag ggctgattga tgctgttccc agcgttaata tcggtaaagc
    19741 cttcgaaaag cctcagaaaa agaacatgcc cagcattcga ccggatcagc tatctcaact
    19801 gatgcagaca atgcgaacag ccagcattag cctttccaca cgctgcctgt tcatgtggca
    19861 acttcttact attacccgcc ctgccgaagc ggctgaagct cgctgggaag aagtagacat
    19921 agaagcgcaa gagtggaaga ttcccgcagc acgcatgaaa atgaaccgcg accatactgt
    19981 tccattgtca gatgaagcaa tagctgtgct ggagatgatg aaaccgctaa gcggcaaccg
    20041 agaatttatc tttcctagcc gtatcaagcc caaccagcct atgaacagtc agactgttaa
    20101 cgcctcacta aaacgcgcag gttttggtgg tgtgcttgtt tcgcatggtc tgagatcaat
    20161 tgccagtacg gctcttaatg aacaaggctt tccgcctgat gccattgagg cagcattggc
    20221 ccatgtggat aagaatgagg ttcgtcgtgc ttataaccgc agcgattacc tggaacagcg
    20281 tcgcccgatg atgcaatggt gggcaaattt tgtgatggca gcggatcgtg gaagcatgat
    20341 tgaaggtggg ataaaaggaa tgaagctagt tgggtgacta aatagctgcg cggcatagtg
    20401 tcccactttc agccagccct tgcaggttaa gccaccagac agtaaacggc aggtgtcgcg
    20461 ttattaggcg cagctattta gtagtaatat taggattggt gattctgcac tccagaagtt
    20521 cggtaatgct gccaacttac tgatttag
//
LOCUS       DADLTF010000070        19916 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.070, whole genome shotgun sequence.
ACCESSION   DADLTF010000070 DADLTF010000000
VERSION     DADLTF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.070"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..746)
                     /locus_tag="JN749_002323"
     CDS             complement(<1..746)
                     /locus_tag="JN749_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7368717.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADSDTDSY
                     MWFETGDNGNEYFKWRHHLTGGRVKDLMNLKWDALYVLVKALFSSEVKISTVNALRIF
                     NSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLVVTGDIF
                     TNRFLINSSTGMWIHMRDQNVIMGRNAVSNDGAQALLRQDHTDRKFVIGGLGNRQFGI
                     YMINNSRTANGTDGQAYMDNNGNWLCGSQV"
     gene            840..1037
                     /locus_tag="JN749_002324"
                     /pseudo
     CDS             840..1037
                     /locus_tag="JN749_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344231.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1131..1319
                     /locus_tag="JN749_002325"
     CDS             1131..1319
                     /locus_tag="JN749_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7368718.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1240..1965
                     /locus_tag="JN749_002326"
     CDS             1240..1965
                     /locus_tag="JN749_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HAY7368719.1"
                     /translation="MPASIPRRNIVGKEKKSRILTKPCVIEYEGQIVGYGSKELRVET
                     ISCWLARTIIQTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETD
                     NRGYMELNRMWLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQ
                     ASNFDFIGSHESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYI
                     RFLNKRARKRLNTKLFKVQPYPK"
     gene            complement(2077..2424)
                     /locus_tag="JN749_002327"
                     /pseudo
     CDS             complement(2077..2424)
                     /locus_tag="JN749_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="JN749_002328"
                     /pseudo
     CDS             complement(2393..4050)
                     /gene="dpiB"
                     /locus_tag="JN749_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4431..5489
                     /gene="citC"
                     /locus_tag="JN749_002329"
     CDS             4431..5489
                     /gene="citC"
                     /locus_tag="JN749_002329"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HAY7368720.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5504..5800
                     /gene="citD"
                     /locus_tag="JN749_002330"
     CDS             5504..5800
                     /gene="citD"
                     /locus_tag="JN749_002330"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HAY7368721.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5797..6705
                     /gene="citE"
                     /locus_tag="JN749_002331"
     CDS             5797..6705
                     /gene="citE"
                     /locus_tag="JN749_002331"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HAY7368722.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACVREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6716..8248
                     /gene="citF"
                     /locus_tag="JN749_002332"
     CDS             6716..8248
                     /gene="citF"
                     /locus_tag="JN749_002332"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HAY7368723.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8252..8803
                     /gene="citX"
                     /locus_tag="JN749_002333"
     CDS             8252..8803
                     /gene="citX"
                     /locus_tag="JN749_002333"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HAY7368724.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8778..9656
                     /gene="citG"
                     /locus_tag="JN749_002334"
     CDS             8778..9656
                     /gene="citG"
                     /locus_tag="JN749_002334"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HAY7368725.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9707..11170
                     /gene="citT"
                     /locus_tag="JN749_002335"
     CDS             9707..11170
                     /gene="citT"
                     /locus_tag="JN749_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HAY7368726.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11284..12090
                     /gene="rna"
                     /locus_tag="JN749_002336"
     CDS             11284..12090
                     /gene="rna"
                     /locus_tag="JN749_002336"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HAY7368727.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12319..12729
                     /gene="rnk"
                     /locus_tag="JN749_002337"
     CDS             12319..12729
                     /gene="rnk"
                     /locus_tag="JN749_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HAY7368728.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(12960..14197)
                     /locus_tag="JN749_002338"
                     /pseudo
     CDS             complement(12960..14197)
                     /locus_tag="JN749_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            14418..14846
                     /gene="uspG"
                     /locus_tag="JN749_002339"
     CDS             14418..14846
                     /gene="uspG"
                     /locus_tag="JN749_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HAY7368729.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="JN749_002340"
     CDS             complement(14967..16532)
                     /gene="ahpF"
                     /locus_tag="JN749_002340"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAY7368730.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="JN749_002341"
     CDS             complement(16777..17340)
                     /gene="ahpC"
                     /locus_tag="JN749_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HAY7368731.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17712..18458
                     /gene="dsbG"
                     /locus_tag="JN749_002342"
     CDS             17712..18458
                     /gene="dsbG"
                     /locus_tag="JN749_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HAY7368732.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKHGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18668..19570
                     /locus_tag="JN749_002343"
     CDS             18668..19570
                     /locus_tag="JN749_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7368733.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19717..19916
                     /locus_tag="JN749_002344"
                     /pseudo
     CDS             19717..19916
                     /locus_tag="JN749_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5016 a         5124 c         4758 g         5018 t
ORIGIN      
        1 ataacttgcg aaccgcaaag ccagttcccg ttattatcca tgtacgcctg accatcggtg
       61 ccattggctg tccttgagtt attaatcatg tagatgccaa attgcctatt ccccagccca
      121 ccaattacga atttacggtc ggtatggtcc tggcgaagca atgcctgcgc accatcgttg
      181 gataccgcat tacggcccat aataacgttc tggtcacgca tatgaatcca catgcctgta
      241 ctgctgttaa ttaaaaaacg gtttgtaaat atatctcctg taacaaccag accatgcccc
      301 atgcttatcc ggccagttct gagattaagc gtaaaggggc gtagtggccc tatattacca
      361 ttttcccctt cattctctcg tgtagggatg atatgcaggc attcttcaga acgacgaaaa
      421 atggcaccaa aagatgaatt aaatattctc agtgcattga ccgtcgatat ttttacttca
      481 ctgctgaaaa gggctttaac aagaacatac agagcatccc atttaagatt catcaggtct
      541 ttaacccggc cgccagtgag gtggtgtctc catttgaaat actcattgcc gttgtcgcct
      601 gtttcaaacc acatgtatga atcagtatcg ctgtcggcat catttttaaa tccaatcttt
      661 gcccagtcag tattccgaat ccaggcaagg attgagtcgt tttcaaaagt aagtccaccg
      721 gacaacgtat cgccattttt ttgcacggcg ttaccagcct tgtttaccgt ttcctgtaaa
      781 ccgaggggcc gtttctggcc tgcatggcat gatttccgct tttaaacggg gaattcagcg
      841 tgctgattgg ctatgtaagg gtatcaacaa atgaccagaa tacagacctg caacgaaacg
      901 ctcttgtttg tgcaggatgt gaataaatat ttgaagataa attaagcgga acaaggacag
      961 accgaccggg attaaaacgt gctttaaagc gccttcaaaa aggaacgcat atagaaaacg
     1021 acgatcgaat caattaaatc gatctgtatt accgatcgat tatgtcccga taaccacacc
     1081 cgactgatga gattttttaa gataacggcg aattgatgca aggaggtgaa atgaaatcaa
     1141 ttcgctgtaa gaactgcaac aaactgttat ttaaagcgga ttcctttgat cacattgaaa
     1201 tcaggtgtcc gcgttgcaaa cgtcacatca taatgctgaa tgcctgcgag catcccacgg
     1261 agaaacattg tgggaaaaga gaagaaatca cgcattctga cgaaaccgtg cgttattgag
     1321 tatgaaggcc agattgttgg ctatggttca aaggagctgc gcgttgaaac catatcctgc
     1381 tggctggccc gcacaatcat tcagacaaag cactattccc gccgttttgt gaataactct
     1441 taccttcacc tgggagtatt cagcgggcgt gatctggttg gcgttctcca gtggggatat
     1501 gcccttaacc ccaactcagg tcgtcgtgtc gtgcttgaaa cggataaccg gggctatatg
     1561 gagttgaatc gcatgtggct acacgacgac atgccccgca actctgaatc acgggccatc
     1621 agctacgcgc tgaaagttat cagattactg tatccgtcag tggagtgggt tcagtccttt
     1681 gctgacgaac gctgcggact cgcaggcgtt gtgtatcagg cgtcgaattt tgattttatt
     1741 ggcagccatg aaagcacgtt ctacgagctg gatggtgagt ggtatcacga gataacgatg
     1801 aacgcgatta agcgcggagg gcaacgaggc gagtatttgc gagctaataa ggagcgtgcc
     1861 gtggtgcaca aatttaatca gtatcgctac atcagattcc tgaacaagcg agcaaggaag
     1921 cggctaaata ccaaactatt caaggttcag ccatacccta agtgatcccc atgtaatgaa
     1981 taaaaagcgg taattaatac tgctgtttca tttgaagcgc gaaagctaaa gttttcgcat
     2041 ttatcgtgaa acgctttcgc gtttttcgtg cgccgcttca cccagccgtt cataggcaat
     2101 tggcttaatg agataatcaa atacaccaca acgtacggct tcagacaccg tttccatatc
     2161 actggctgca gtggtaaaca ccacgtcgcc gggataatgc gcctgcacca gttcatgcag
     2221 taaattaatc cctctaccgt caggaagata gttatcgagc aagattagcc ccggcttaaa
     2281 acgctcgatc atcattcggg cctgcgccag gtttcccgcc agtaatatct gactgaatcc
     2341 gggaatgtga cgaatatatt ccgcatgcat ctctgccagc ggcgtttcgt cctcaacgat
     2401 caatagggtt aatggagctg tcattaggtt tcactttcgg aatatagatt gaaaataagg
     2461 taccgcaggg atcattatct tcgagagtga taacaccacc gcagcgcgtt acgtagctgg
     2521 caatcaagta caacccaatg ccatgttcac cgggctcgtc agcacgcgta ctgacccctg
     2581 ctcaaatatt ttgtctcgta gagactctgg aacgccgcag ccctgatcgg cgacttcaat
     2641 caccacatca tcgccttcat cgctgaggaa taattcaacg ctcttgtttc cttcatcgct
     2701 acgcaggctg gcttcgaagg cgttatcaag taaattgcca acaatggcgg caaactcggt
     2761 gctatccagt cctggcggca gttgcgaaag ctggctaccg gggacgatga tcatttttag
     2821 ccccagttcg cgggcgcgct gcactttacc aaaaagcagc cccgccacct ggcgatcggc
     2881 aaacgcctcg cgcaggctgt caataagctg ttgctgggcc tgagactccc cctgcaccat
     2941 cgccagcacg cgatcatact ctttcatctg caacagacca ttgagagtcg acatccaatt
     3001 gagatgctcg tgtcgcaatg tacgaaggct ctcaacgtat tgttttattt gcgtcagttg
     3061 cgcattgagg gtggaaattt cgtctttact gcgaaagctg ataatggctc ccagcaaatc
     3121 atcacctgaa cgaatagctt cccgattggc aataacgctc agaccgttaa agttagccac
     3181 cacatcctga cgtttttcat caatctgttc ggtaaagaaa tcggcagacc tgaccacttc
     3241 agcaatgggt ttacccaacc attgccgtcc aggggaactc agccccagca tctttcttgc
     3301 gttacgattg atggcggtaa tgtaaccgtg cggatccacc gcaatcagcc cttcataaac
     3361 cgaactaaac agcgcctctt gctgacggac cacccgtgcg atttgctttg gctccatgcc
     3421 catcatctgc cgacggatat gcgcggccag gaaccacgac agcaacatca gaatccctaa
     3481 cagcacgaca aacacacctg ccatcggtaa taaaaactca gcccgccagc tatcgatttt
     3541 actcaccagg tagccaatcg acaccacgcc gatgactttt ccatcgtcat caaagattgg
     3601 cgttttggcg cgcatcgcca tacccattga ccctttcccg gtaatgaagt agctctcccc
     3661 tttctccagc gcgccctgtt tggtgaactg cataggataa ccaattttct ccggattagg
     3721 atggtaaagg cggatcgagt gccggtcgcc aatcaccaca taatcaaaat cggtatctct
     3781 ttgtaatttg ttagcgatgg tcgccagccg tttgtagtca cgcgttttca ccgcagagat
     3841 gacactgtca ttggaggcaa taattttcgc ctgattcatt gccatgtcgc gtacatgaag
     3901 cgttaaatag tcctcaaaac tggccgtaaa atattgcgcc agggctgcaa tgacaaatat
     3961 tgagaacacc agaatcagca aaaagatacg cagcggaaat gtcagtcttt ggaaaaatgc
     4021 aaactgttta ttctcgttaa gctgcaacat tgttttcctt gcgggttgtc ccgagcctct
     4081 ttgacggatg taattaaaat gttgaatcaa aatacataga tattaattat gatcgtggca
     4141 aattttattc attaaatcaa ttaaatcact taaacctgcg agtcatttcg taattacatt
     4201 aattaaaaaa cttaaaacca ttaaacaaat aaaaaaacca ctaactctat gtgaaataaa
     4261 tcaaaatttc acgccgaaat actccttagg atgtatagcg aaaggagaaa aagatatacc
     4321 tcgatcaccc ccctttctcc caagtgaaaa taaaaggtta tcagtttgca acattgaaca
     4381 acattcgttg caaatcgata acaacatgca ccttcaggat actatttatt atgttcgtca
     4441 atgatatttt cacccgcgta aaacgttcag aaaataaaaa aatggcggaa atcgcccaat
     4501 tcctgcatga aaatgatttg agcgttgaca ccacagtcga agtatttatt accgtaaccc
     4561 gcgatgaaaa gcttatcgcg tgcggtggaa ttgccggaaa tattattaaa tgcgttgcta
     4621 tcagtgaatc cgttcgcggt gaaggactgg cgctgacatt agccactgaa ctgataaacc
     4681 tcgcctatga gcggcacagc acgcatctgt ttatttatac caaaaccgaa tacgaggcgc
     4741 tgttccgcca gtgcggtttt tccacgctga cctgcgtacc cggcgtgatg gtgctgatgg
     4801 aaaacagcgc cacgcgactg aaacgctatg ccgaatcgct gaaaaaattg cgtcatccag
     4861 ggaacaagat tggctgcatt gtgatgaacg ccaatccctt tacgaatggt caccgttatc
     4921 tgattcaaca agctgcggca cagtgcgact ggtttcatct gtttttagtc aaagaagatt
     4981 cttcacgctt cccctatgaa gaccggctgg atctggtgtt aaaaggcacc gccgatattc
     5041 cacgcctgac tgtgcatcgc ggctcagaat acatcatctc ccgcgctacg ttcccttgct
     5101 acttcattaa agaacagagc gtcattaacc attgttacac cgaaattgat ctgaagattt
     5161 tccgtcagta cctcgctccc gcgcttggtg taactcaccg ctttgtcggt actgaaccct
     5221 tttgtcgcgt taccgcccag tacaaccagg atatgcgcta ctggctggaa acgccgacta
     5281 tctccgcagc gcccatcgaa ctggttgaaa ttgagcggct gcgttaccag gagatgccga
     5341 tatccgcttc ccgggtacgt caactgctgg cgaaaaacga tctcacggct atcgcgccgc
     5401 tggtccctgc agtcacgctg cattatttgc agaacctgct tgaacactcc cgccaggacg
     5461 cggcagctcg tcaaaagacc cccgcatgag aaacaggtga aaaatgaaaa taaaccagcc
     5521 cgccgttgca ggcacccttg agtctgggga tgtgatgata cgcatcgccc cactcgatac
     5581 gcaggatatc gacctgcaaa tcaatagcag cgttgagaaa cagtttggcg atgcaattcg
     5641 caccaccatt ctggacgttc tcgcccgcta caacgtgcgc ggcgtacagc tgaatgtcga
     5701 tgacaaaggc gcactggact gcattttacg tgcacgactg gaagccctgc tggcacgcgc
     5761 cagcggtatc ccggctctgc catgggagga ttgccaatga tttccgcttc gctgcaacaa
     5821 cgtaaaactc gcacccgccg cagcatgttg tttgtgcctg gtgccaatgc cgcgatggtc
     5881 agcaactcct tcatctaccc ggctgatgcc ctgatgtttg acctcgaaga ctccgtggca
     5941 ttacgtgaaa aagacaccgc ccgccgcatg gtttatcacg cgctgcaaca tccgctgtat
     6001 cgcgatattg aaaccattgt gcgtgtcaac gcgctggatt ccgaatgggg tgttaacgac
     6061 ctggaagccg tcgttcgcgg tggtgcggac gttgtgcgtc tgccgaaaac cgataccgct
     6121 caggatgttc tggatattga aaaagagatc ctgcgtatag aaaaagcctg tgttcgtgaa
     6181 cccggcagca ccggcctgct ggcggcgatt gaatctccgc tgggcattac ccgcgcagtg
     6241 gaaatcgctc acgcttccga gcgtttgatc ggtatcgccc tcggtgcaga agactatgtg
     6301 cgcaacctgc gtacagaacg ctccccggaa ggaactgaac tgctgttcgc acgctgttcc
     6361 attttgcagg ccgcgcgctc tgcgggtatt caggcgttcg ataccgtcta ttccgacgct
     6421 aacaacgaag ccggattcct gcaagaagcc gcccacatca aacagctggg ctttgacggc
     6481 aaatcgctga tcaacccgcg tcagattgat ctgctgcaca acctctacgc accgacccag
     6541 aaagaagtgg atcacgcccg ccgcgtcgta gaagccgctg aagccgccgc tcgcgaaggc
     6601 ctcggcgtgg tttccctgaa cggtaagatg gtggacggtc cggttatcga tcgcgcccgt
     6661 ctggtgctct cccgtgcaga actttccggc atccgcgaag aataaggcaa tcaaaatgac
     6721 gcagaaaatt gaacaatctc aacgacaaga acgggtagcg gcctggaatc gtcgcactga
     6781 atgcgatctt gccgctttcc agaactcacc aaagcaaacc taccaggctg aaaaagcgcg
     6841 cgatcgcaaa ctgtgcgcca acctggaaga agcgattcgt cgctcaggtt tacaggacgg
     6901 catgacggtt tccttccatc acgctttccg tggcggtgac ctgaccgtca atatggtgat
     6961 ggacgtcatc gcgaagatgg gctttaaaaa cctgaccctg gcgtccagct ccctgagtga
     7021 ttgccatgcg ccgctggtag aacacattcg ccagggcgtg gttacccgca tttatacctc
     7081 tggcctacgc ggtccactgg cggaagagat ctcccgtggt ctgctggcgg aaccggtgca
     7141 gatccactct cacggcggtc gtgtgcatct ggtacagagc ggcgaactga atatcgacgt
     7201 ggctttcctc ggcgtcccgt cctgtgatga attcggtaat gccaacggct acaccggtaa
     7261 agcctgctgc ggctccctcg gctatgcaat ggttgatgcc gacaacgcaa aacaggtcgt
     7321 gatgcttacc gaagaactgc tgccttatcc gcataatccg gcaagcattg agcaagatca
     7381 ggttgatttg atcgtcaaag ttgatcgcgt tggcgatgct gcaaaaatcg gcgctggtac
     7441 tacccgtatg accactaacc cgcgcgaact gcttattgcc cgtagcgctg cggatgtgat
     7501 tgtcaactct ggctacttca aagaaggttt ctccatgcaa accggcaccg gcggcgcttc
     7561 gctggcggta acccgtttcc tggaagacaa aatgcgtagc cgcgatattc gcgccgactt
     7621 cgccctcggc ggtattaccg cgacgatggt tgatctgcac gaaaaaggtc tgatccgcaa
     7681 actgctggat gtgcagagct ttgacagcca tgctgcgcaa tcgctggccc gtaaccccaa
     7741 tcacatcgaa atcagcgcca accagtacgc taactggggt tcgaaaggcg catcggttga
     7801 tcgtctcgac gtggtggtac tgagcgcgct ggaaattgac aggcagttca acgttaacgt
     7861 gctgaccggt tctgacggcg tactgcgtgg cgcttccggt ggtcactgcg ataccgcgat
     7921 tgcctctgcg ctttccatca tcgtcgcgcc actggtacgc ggtcgtattc cgactctggt
     7981 ggataacgtg ctgacctgca tcaccccggg ctccagtgtc gatattctgg tcacagacca
     8041 cggtatcgca gttaacccgg cacgtccgga actagcagaa cgtctgcagg aagcgggcat
     8101 taaagtggtt tccattgagt ggctgcgcga acgtgcgcgt ctgctgaccg gtgaaccaca
     8161 gccgattgaa ttcaccgacc gcgtcgttgc cgttgtgcgt taccgcgatg gctcggtgat
     8221 cgatgttgtg catcaggtga aggaataagc catgcacctg cttcctgaac tcgccagcca
     8281 ccatgcggtg tcaattcccg agctgctcgt cagccgggat gaaaggcaag cacggcaaca
     8341 cgcctggctc aagcaccatc ctgttccact ggtctccttt accgtggttg cgcctgggcc
     8401 gattaaagac agcgaggtca cacgccgaat ttttaatcat ggcgtgacag ccttgcgtgc
     8461 cttagccaca aagcagggct ggcaaattca ggagcaggct gcactggttt ccgccagcgg
     8521 gccggagggc atgttgagca ttgccgcccc ggctcgcgat ctcaagctcg ccaccattga
     8581 gcttgaacat agtcatcctc tcgggcggtt atgggatatc gatgtcctga cgcccgaagg
     8641 cgaaattctc tcccgccgcg actattcact gccgcctcgc cgctgcctgt tgtgcgagca
     8701 aagcgcagcc gtctgcgcgc gtggaaaaac ccatcaactg accgatttac tcaaccgcat
     8761 ggaggcactg ctgaacgatg tcgatgcctg caacgtcaac taaaaccaca aagcttgcga
     8821 cgtcattaat cgatgagtac gccctgctgg gctggcgcgc catgctgact gaagtcaatc
     8881 tgtcaccgaa accaggcctc gtggatcgca ttaactgcgg tgcgcacaaa gatatggcgc
     8941 tggaagattt ccaccgcagc gcgctggcga ttcagggctg gttaccccgt ttcattgaat
     9001 ttggtgcctg tagtgcagaa atggcaccgg aagcggtact ccacggatta cgcccaattg
     9061 gtatggcttg cgaaggtgat atgttccgcg ccactgcggg cgtaaacacg cataaaggca
     9121 gcattttttc tttagggctg ctatgtgcgg caattggccg tttgcttcag ctcaaccagc
     9181 cggttacgcc aacaaccgtt tgttctacgg cggcaagttt ctgccgtggc ctgaccgatc
     9241 gcgaactgcg taccaataat tcacgactga cggcaggtca acggttgtac cagcagcttg
     9301 gccttaccgg cgcacgcggt gaagccgaag cgggttatcc actggtgatc aaccacgcct
     9361 tgccgcatta cctcactctg ctggatcagg ggttagatcc tgaactggca ttgctcgata
     9421 ccttgctcct actgatggct accaacggcg ataccaacgt tgcatcgcgc ggtggcgagg
     9481 ggggcctgcg ctggctacag cgcgaggcgc aaacattatt gcaaaaaggg ggcattcgaa
     9541 cccccgccga tctcgattat ctccggcagt tcgacaggga gtgtatcgaa cgaaatctca
     9601 gtccaggcgg cagtgccgac ctgctgatcc ttacctggtt tttagcacag atttaattat
     9661 ttaagcactt gataaatttg gaaatattaa ttttcggaga acccgtatgt ctttagcaaa
     9721 agataatata tggaaactat tggccccact ggtggtgatg ggtgtcatgt ttcttatccc
     9781 tgtccccgac ggtatgccgc cgcaggcatg gcattacttc gctgtgtttg tggcaatgat
     9841 tgtcggcatg atcctcgagc caattccggc aacagcgatc agttttattg cggttactat
     9901 ttgcgttatt ggcagtaatt acctgctctt tgatgccaaa gaattagctg acccagcgtt
     9961 taatgcgcaa aaacaggcgc tgaaatgggg cctggctggt ttttccagca ccacggtatg
    10021 gctggtattt ggcgcattta tttttgcatt agggtatgaa gtttccgggt taggtcgtcg
    10081 cattgccctt ttcctggtga aattcatgga caaacgcacg ctgacgttgg gttatgcgat
    10141 tgtcattatc gacattctgc tggcaccgtt tacaccgtcc aacaccgcgc gtaccggggg
    10201 tacggttttt ccggtcatta aaaacctgcc gccgttgttt aaatcattcc cgaacgatcc
    10261 gtccgcgcgt cgtattggcg gctatttgat gtggatgatg gtcattagta ccagtctgag
    10321 ttcatccatg tttgtcaccg gtgcggcacc aaacatgctg ggtctggagt tcgtcagcaa
    10381 aattgccggt atccagatta gctggttgca gtggttcctc tgcttcctgc cggttggggt
    10441 tatcttgctt atcattgcgc cgtggctttc ctacgtgctg tacaaaccgg aaatcacaca
    10501 cagtgaagaa gtggcaacct gggcgggtga tgaactaaaa accatgggtg cgctgacacg
    10561 cagagagtgg acgctgattg gccttgtatt gctcagctta ggtttgtggg tatttggcag
    10621 tgaagtcatt aatgctactg cggttggtct gctggcagtt tcgctaatgc tggctctgca
    10681 cgttgtgccg tggaaagaca ttacccgcta taacagcgca tggaacacgc tggtcaacct
    10741 ggcaactctg gttgtgatgg ctaacggcct gactcgttct ggttttattg actggttcgc
    10801 cggtaccatg agtacgcacc tggaaggatt ctcaccaaac gcaacggtga ttgtactggt
    10861 tctggtgttc tactttgcac actacctgtt tgccagcctg tctgcgcaca ccgcaaccat
    10921 gctgccggtt attctggccg tcggtaaagg tattccgggc gtaccaatgg aacaactgtg
    10981 tatcctgctg gtgctgtcta tcggtatcat gggctgtctg acgccgtatg caaccggtcc
    11041 tggggtgatt atttacggct gtggctatgt gaaatcaaaa gattactggc gtcttggcgc
    11101 aatcttcggg gtgatttaca tctctatgtt actgttggtt ggctggccga ttctcgccat
    11161 gtggaactaa tacgttcgcc aaacgctgaa taaatcatca cgcccgccag gtaagcccac
    11221 ctggcgggct tttttatgat ttaatagata gtattcagtg tatgtatgag ttccacaccc
    11281 attatgaaag cattctgccg taacgccgcg ttgctcgcgg tttctctgct tcccttatct
    11341 tctgccaacg ccgtagcgtt gcaggcaaaa cagtatggcg attttgatcg ctatgtcctg
    11401 gccctctcct ggcaaaccgg attttgccag agtcaacacg atcgaaatcg taacgaacga
    11461 gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg attttctgac cgtacatggt
    11521 ctgtggccag gattgcctaa atcggttgct gcccgtggtg ttgatgaacg ccgctggatg
    11581 cgcttcggtt gcgctactcg cccaatcccg aatctaccag aagcacgcgc cagccgaatg
    11641 tgttcatcgc cggaaaccgg gttatcactg gaaacggccg ctaaactaag tgaagtcatg
    11701 ccaggagctg gcggacgttc ctgcctggaa cgctacgaat atgccaaaca cggtgcctgc
    11761 tttggttttg atccggacgc atacttcggt acgatggtac gcctgaatca agaaattaaa
    11821 gagagcgaag ccggaaaatt ccttgcggat aattacggta aaacagtgag ccgccgtgac
    11881 tttgacgccg cctttgccaa aagctgggga aaagagaacg tgaaagcagt taagctaacg
    11941 tgccagggta accctgcgta tttgactgaa attcagatct cgatcaaagc tgacgccatc
    12001 aacgctccgc tttctgcaaa ctcattcttg ccacaacctc acccaggtaa ctgtggcaaa
    12061 acctttgtga ttgataaagc gggttattaa tcctctgatt tacaaaacac gctaattcgt
    12121 cttccataac catgccgatt aagaattata cataatgcgc tgcattgagg aattccctga
    12181 atgtgacgca aatcacttca aagagtgagc aacagtcagt atcatcatgt gagttaaacc
    12241 ctcgccgcct gacggtgagg gttttctttt gggtatgttt atctgcgaca ctcgcagtac
    12301 cgacgacatg gagtaaaaat gtccagacca actatcatca ttaacgacct ggatgccgaa
    12361 cgcatcgata ttctgctgga gcaacccgcc tatgctggtt tgccaatcgc cgacgcgtta
    12421 aacgcagagt tggatcgcgc ccaaatgtgt tcgccagaag agatgccaca cgacgtggtg
    12481 acaatgaaca gccgggttaa attccgcaat cttagagatg gcgaagtgcg tgtgcgcacg
    12541 ctggtgtatc cggcaaaaat gaccgatagc aatactcagc tttccgttat ggctccggta
    12601 ggtgccgcac tgctggggct gcgcgttggc gattccattc actgggaact tccgggcggc
    12661 gttgcaaccc accttgaagt gctggaactc gaataccagc cagaagctgc tggcgactac
    12721 ctgctttaat cccatctgca caagcgataa ctcgctttgc ccagaggatg cactcgcatc
    12781 ctctttacgc aaaatcagca atttttcctg gttctcactg ttcacttttg ctgcaaaatt
    12841 attccgtttc gtttgtggga ataaccatgg cagaagcatt ttacatcctg ataggatttc
    12901 tgattatggc ggcgattatc gtcatggctg tcctttacct cgaaaaccac tcctgacgat
    12961 catattgttc cccccggcat cgcattcacc agacctgaaa ccgccttctg cgccgcctct
    13021 gcgctttgtg caccgggtac cagaatcacc ttacggcact cctcttcacg tttttcgaaa
    13081 atctcatatc cccgggcggc ctcttcaaac ggcatatagt gggtaacaat ttcttctggt
    13141 ttcagtaatc ctttctcaat taacggtaat aattctccca gccatgcgtg aacgtgggtc
    13201 tgtcccattt taaacgtcaa ccctttatca aaggcgtcgc caaacaggaa accgtgaata
    13261 aatccagcgt agacgcccgg tacgctaaca atgccgccac gcctgaccgc cgcaatacac
    13321 tggcgcaacg ctttaccgct gctgccctcc agtttcaggt tagtcagcac cgtttccgtg
    13381 gtgctgcctt tcgcttcaaa accgacggcg tctattactg catccacgcc ccggtgacct
    13441 gccgtttgtt caataattga ctgtgccgga tcgctatctt catcgaaatt aatcgggatc
    13501 gcgccgtagc ggtcagcggc gaaacgcaag cggtagggat gatgatcaac aacaaaaatc
    13561 tgttccgcac cgagcaaccg tgcacaggcg attgtcaaca atcccacagg accagcacca
    13621 tagactgcaa cgcttgaacc ttgttggatc tgcacatttt ttgctgcctg ccatgccgtt
    13681 ggcagaatat cagaaaggaa aagggcttta tcatctgaaa gcaaaggcgg tactttaaac
    13741 ggccccacat tccctttagg gacgcggaca tattccgcct gcccgctagg aacgccacca
    13801 tacaggtgac tataaccaaa caatgccgcg ggagcaggta tctgtttttt attgagcgca
    13861 gcgcctttac ccgcattggt attttcgcag gcggcatatt gctgtaatcg acagaaaaaa
    13921 cagtcgccac aagcaatgac gaacggaatt accacccggt cgcctttttg caaatttttt
    13981 acgtccttcc cggtttcaac gacttcccca taaattcatg accaaaaata tcgccatgtt
    14041 taacctgggg tattttgcct cgataaagat ggaggtcaga gccacagatc gccgttaccg
    14101 taatacgcag aataatatca tctgcctgtt caatgcccgg atcgggaaca ttttctacct
    14161 gaacgtgatg tgggccgtga taagtcaatg ctttcatgca acctccagag acagttcaaa
    14221 aaagagtcag tctttaaggg tagctatcac agcgggaaaa cgggcggtac cgcaggtttt
    14281 gggagaattc ctggtgttca gtgagaatat attaatcaca gaaaccgttc gtaaggaaaa
    14341 agaaccagac aaatatcagg gataaatatt ataatcatgg cgaatgattt gttcatgatt
    14401 aacagggaga aagggttatg tataaggcaa tcattatgcc agttgatgta tttgaaatgg
    14461 aattgagcga caaagctgtt cgccacgctg aattcctcgc ccaggatgac ggagttattc
    14521 atctacttca cgtactaccc gggtcagcca gcctgagcct gcaccgtttt gccgctgatg
    14581 tgcgtcgttt tgaagagcat ctgcaacatg aagcagaaga acgtctgcaa acgatggtca
    14641 gccacttcac catcgatcct tcccgcatta aacaacatgt ccgttttggt agcgtgcggg
    14701 atgaagtcaa tgagttggca gaagaactgg gggctgatgt tgtagttatc ggttctcgca
    14761 acccatcgat ttcgacccat ctgttaggtt ctaacgcctc gagcgtgatc cgccacgcca
    14821 atctgcctgt actggtcgtt cgctaacaga taaaagtatt tatgccgccc tgcctggaac
    14881 atcccggcag ggctttttaa tgccataaaa aagccgccag gtttgacccc ggcggctaag
    14941 caattgcagg tgaatcttac ttcttcttat gcagttttgg tgcgaatcag gtagtcaaaa
    15001 gcactcagag aggctttggc accttcgcca gtggcgatga tgatctgctt gtacggaacc
    15061 gtcgtacagt cacccgctgc gaacacgcct ttgacgttgg tttcacattt cgcatcaatg
    15121 ataatctcgc ccatgcggtt acgttcgact gcgccttcca gccagttggt gttcgggagc
    15181 agaccaatct ggacaaaaat accggccagt tcgatgttgt gaatattgcc gctgacacga
    15241 tctcgatatt ccagaccaac gactttgctg ccgtcgcctt tcacttccgt ggtttgcgca
    15301 ttcagaataa tgtcgacgtt tttcaggctg cgcagtttgt cctgcagaac ctggtcggct
    15361 ttcatttctg gcgcaaattc cagcagcgtt acgtgctcaa cgatacccgc caggtcgatt
    15421 gccgcttcca cgccggagtt accgccgccg ataaccgcta cgcgtttgcc tttaaacagc
    15481 gggccgtcgc agtgcgggca gtaggtcacg cctttagtgc gatactgatc ttcgcccgga
    15541 acgttcatgt tgcgccattt tgcacccgtc gccacgataa tgctgcgtgc tttcagtacc
    15601 gcgccagaag ctgtttcaat ctggtgtaga ccaccttcaa ccgctgccgg gatcagtttg
    15661 ctggcgctct ggctgtcgat cacatcaacg tcgtattcat caacgtgaac tttcagtgcg
    15721 cctgccaact tctgcccttc agtcttcggt acagaaatgt agttttcgat atcaacggta
    15781 tcgaggatct gaccaccaaa acgttcgccc atcagaccgg tacggatgcc tttacgtgcg
    15841 gagtaaattg ctgccgctgc acccgccggg ccggaaccaa cgattaatac gtcataagca
    15901 tcacgcttgt tcagctcttc tgccgcacgt ttttccgcgc cagtatcaat tttggcaacg
    15961 atttcagtca acgtcatgcg gccctgacca aactctttcc cgtttacgaa cactgccgga
    16021 acgcccatca cgttgcgatc ggtgatttcg ttctggaagg tgccgccgtc aattgcagtg
    16081 tgcttgatgc gcgggttcaa tacgctcatc aggttcagcg cctgcaccac gtccgggcag
    16141 ttatggcaag agagcgagta ataggtttcg aattcaaaat caccgtcaat atggcgaatc
    16201 tgctccagca gagactgcgc ttctttcgac ggatgaccac cggtccacag caacgccagt
    16261 accagcgagg tgaactcgtg gcccagcggg gagcctgcaa aacgtggtcc ctggttggaa
    16321 cctgggttgg tgatcaggaa agacggctta cgcactggca agctgttatc ttctttaaag
    16381 gtgactttgt ctgacagttc tgcgatttca gccaacagtt ccttgatttc tgccgattta
    16441 gcgctgtcat ccagcgtggc aattaactca acaggcttgg tcaatttctc aaggtaagcc
    16501 ttgagttgag ttttcatatt tgtgtcgagc atgtttatct cctgggcttt aaacatcatc
    16561 atgcaagcgg cctctgcggc tacctggatg cagcttgcat catggtgcgg ggaagaaaat
    16621 cgggtgccgc agcacccgaa gaattaggtg aatttccgaa gtatttcgcg tcagagcaag
    16681 gcgactagcc tgtgaatccc cgggagctta cacaagtaag tgaccggggt gagcgggcga
    16741 cgccaacgcc gctatggcgt gaaagacgaa ggaaatttag attttaccaa ccaggtccag
    16801 agacggagcc agagttgctt caccttcttt ccatttagcc gggcaaactt cacctgggtg
    16861 agaagctacg tactgagctg ctttgatttt acgcagcaga tcagacgcgt cacggccaat
    16921 gccttcagcg gtaacttcga ttgcctggat gataccctgc gggtcaacaa cgaaggtcgc
    16981 acggtcagcc agaccttcat cttcacgcat gttgtcgaag ttacgggtca gggcgccagt
    17041 cgggtcgccg atcatcgcat atttgatttt agcgatggtt tcagagctgc tgtgccatgc
    17101 tttgtgggtg aagtgagtat cggtagatac tgcgtatacg tctacgccca gtttctgcag
    17161 ttcttcgtag tggtcagcaa cgtcacccag ttcggtcggg catacgaaag taaagtcagc
    17221 cgggtagaag aagaagacgc tccagcggcc ttcggtatct ttttcggtga tttcgatgaa
    17281 ttcgccgttt ttgaatgcct ggtttttaaa aggtttaatt ttggtgttaa tcaaggacat
    17341 ctatacttcc tccgtgtttt cgatgagatg taaggtaacc aatttttgcc gattcggcta
    17401 atgcgtttcc attatcaaat cgataagttt taccttacaa ccttcgtaag acaactttgt
    17461 gaactttgca ccgccagcgg cgataaaaag taaaatggcc ccctttccag gtgaccgtgt
    17521 aatctgaact accctttggt atcttcagcg cctgcctgca ggctgaggtg ttgcccttac
    17581 aaatgcaaca acgacatgga ttacaacacc ctcataaaca aagggcaatc acctgatcta
    17641 agctcttacc tatgacagtg ataggttatg ccttttactc gacttttgca ctgactgaaa
    17701 agaacaaatt aatgttaaaa aagatacttt tactggctct gcttcctgca atcgccttcg
    17761 cagaggaact tccttcccca gtaaaagcga ttgaaaaaca tggcattaca atcatcaaaa
    17821 cgttcgatgc ccccggagga atgaaaggtt atctcggaaa gtatcaggat atgggcgtca
    17881 ccatctacct gactcccgat ggtaagcacg ctatctctgg ttacatgtac aacgagaaag
    17941 gtgaaaacct gagtaacaca cttatcgaaa aagaaattta cgcaccagcc ggacgcgaaa
    18001 tgtggcaacg gatggaacaa tcccactggc tcctcgacgg taaaaaagat gcgccggtca
    18061 ttgtctacgt cttcgccgat ccgttctgcc catattgtaa acagttctgg cagcaggcgc
    18121 gcccgtgggt agattctggc aaagtgcaat taagaacatt gttggttggg gttatcaagc
    18181 cagaaagccc ggcgacagca gcggcaattc ttgcctccaa agatcccgca aaaacctggc
    18241 aacaatatga agcctctggt tgcaagctta agctaaacgt gcctgcaaac gtaagtacag
    18301 agcaaatgaa agtgttaagt gacaatgaga aactgatgga cgatctgggg gcaaatgtca
    18361 cgccggctat ctattacatg agtaaggaaa atacgctgca acaggccgtg gggttgcccg
    18421 atcagaaaac gcttaatatc attatgggaa ataaataaaa ggtaattcat ggatggttga
    18481 actatatccc tgcttttcaa ccatcctaaa aaagcatgaa ttgctcgctc taccctaagt
    18541 ggctgtgcta ttttagaaaa acttaatcct gagttaagca aaaaaacata atcaataaaa
    18601 atacatacgc aaaaaacatt gattaagtga atataccatg gaagaaaaat ataaaccgga
    18661 gtagtgtatg gccaatctct acgacttgaa aaagttcgac ttaaatcttc ttgtcatatt
    18721 tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat atatcacccc
    18781 atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat
    18841 tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc accatcttga
    18901 gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa
    18961 aaactttatt atctacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg
    19021 ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga
    19081 gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt
    19141 gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc taatttgtag
    19201 taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt
    19261 cacccaatta atatccaaat cagcaggagt tgatgatatt caaatggaaa tagacgagaa
    19321 gtttatgaat cgcaaaattt catttcgtgg ttcctctttg ctgacaataa ttaatagcat
    19381 tgcagttaca gatttactgg gaatagtccc ctatgaactt tataactacc accgtgactt
    19441 tcttaattta aaagagataa aactagaaca tccgttacct tcaataaagc tttacatttc
    19501 ctataataaa tcttctttaa ataatttggt gttctcccga tttattgacc gtctaaatga
    19561 gagtttttga tatataacta cagacagcta ctataacttt catctattta ttcacagctc
    19621 gaatgaaaaa taaaacactc tgctttacag tgtttacttt ataattaaca gtcttatctc
    19681 actgttcacc ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt
    19741 ccactcaata ttctggaagc tgcacgagag cgtattacct agacactcaa caccctaccc
    19801 cgcgtttgtg tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc
    19861 gaacttgccc gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggc
//
LOCUS       DADLTF010000071        19844 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.071, whole genome shotgun sequence.
ACCESSION   DADLTF010000071 DADLTF010000000
VERSION     DADLTF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.071"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(15..91)
                     /locus_tag="JN749_002345"
     tRNA            complement(15..91)
                     /locus_tag="JN749_002345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55..57),aa:Arg,seq:acg)
     gene            complement(95..187)
                     /locus_tag="JN749_002346"
     tRNA            complement(95..187)
                     /locus_tag="JN749_002346"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(151..153),aa:Ser,seq:gct)
     gene            complement(503..688)
                     /gene="csrA"
                     /locus_tag="JN749_002347"
     CDS             complement(503..688)
                     /gene="csrA"
                     /locus_tag="JN749_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HAY7368734.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(923..3553)
                     /gene="alaS"
                     /locus_tag="JN749_002348"
     CDS             complement(923..3553)
                     /gene="alaS"
                     /locus_tag="JN749_002348"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAY7368735.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3681..4181)
                     /gene="recX"
                     /locus_tag="JN749_002349"
     CDS             complement(3681..4181)
                     /gene="recX"
                     /locus_tag="JN749_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAY7368736.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4424..5485)
                     /gene="recA"
                     /locus_tag="JN749_002350"
     CDS             complement(4424..5485)
                     /gene="recA"
                     /locus_tag="JN749_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAY7368737.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5565..6062)
                     /gene="pncC"
                     /locus_tag="JN749_002351"
     CDS             complement(5565..6062)
                     /gene="pncC"
                     /locus_tag="JN749_002351"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HAY7368738.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(6207..7292)
                     /gene="mltB"
                     /locus_tag="JN749_002352"
     CDS             complement(6207..7292)
                     /gene="mltB"
                     /locus_tag="JN749_002352"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HAY7368739.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7548..8111
                     /gene="srlA"
                     /locus_tag="JN749_002353"
                     /gene_synonym="gutA"
     CDS             7548..8111
                     /gene="srlA"
                     /locus_tag="JN749_002353"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAY7368740.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8108..9067
                     /gene="srlE"
                     /locus_tag="JN749_002354"
                     /gene_synonym="gutE"
     CDS             8108..9067
                     /gene="srlE"
                     /locus_tag="JN749_002354"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAY7368741.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9078..9449
                     /gene="srlB"
                     /locus_tag="JN749_002355"
                     /gene_synonym="gutB"
     CDS             9078..9449
                     /gene="srlB"
                     /locus_tag="JN749_002355"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAY7368742.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9453..10232
                     /gene="srlD"
                     /locus_tag="JN749_002356"
     CDS             9453..10232
                     /gene="srlD"
                     /locus_tag="JN749_002356"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HAY7368743.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10337..10696
                     /gene="gutM"
                     /locus_tag="JN749_002357"
     CDS             10337..10696
                     /gene="gutM"
                     /locus_tag="JN749_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HAY7368744.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10763..11536
                     /gene="srlR"
                     /locus_tag="JN749_002358"
                     /gene_synonym="gutR"
     CDS             10763..11536
                     /gene="srlR"
                     /locus_tag="JN749_002358"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HAY7368745.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11529..12494
                     /gene="gutQ"
                     /locus_tag="JN749_002359"
     CDS             11529..12494
                     /gene="gutQ"
                     /locus_tag="JN749_002359"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HAY7368746.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSSTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12491..14005)
                     /gene="norR"
                     /locus_tag="JN749_002360"
     CDS             complement(12491..14005)
                     /gene="norR"
                     /locus_tag="JN749_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HAY7368747.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGNDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14192..15631
                     /gene="norV"
                     /locus_tag="JN749_002361"
     CDS             14192..15631
                     /gene="norV"
                     /locus_tag="JN749_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HAY7368748.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15628..16761
                     /gene="norW"
                     /locus_tag="JN749_002362"
     CDS             15628..16761
                     /gene="norW"
                     /locus_tag="JN749_002362"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HAY7368749.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16889..19141)
                     /gene="hypF"
                     /locus_tag="JN749_002363"
     CDS             complement(16889..19141)
                     /gene="hypF"
                     /locus_tag="JN749_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HAY7368750.1"
                     /translation="MAKNTFCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQVVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19294..19821)
                     /gene="hydN"
                     /locus_tag="JN749_002364"
     CDS             complement(19294..19821)
                     /gene="hydN"
                     /locus_tag="JN749_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HAY7368751.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISCDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
BASE COUNT         4669 a         5526 c         5058 g         4591 t
ORIGIN      
        1 tatcaagtaa gagatggtgc atccgggagg attcgaacct ccgaccgttc ggttcgtagc
       61 cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg attcgaaccc
      121 cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc ctctcggcca
      181 cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca tcgtcgctgc
      241 gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccca cacttttatc
      301 gtttgcacac ttcacgctca attagtctgt aaaaacgtca aaaagagtgt tttatctaca
      361 gaaaaacgga ggtctggcag atagcagtaa tgcaaaaaaa tggagactta agttgaatga
      421 acgggagtaa agcgaaaaga ctatagagtg aaggagaaat tttgagggtg cgtctcaccg
      481 ataaagatga gacgcggaaa gattagtaac tggactgctg ggatttttca gcctggatac
      541 gctggtagat ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg ccaatacgta
      601 cctggttgcc ctttacccct aaaactgtca cggtgacctc atccccaatc atgagggtct
      661 caccaactcg acgagtcaga atcagcattc tttgctcctt gaaagattaa aagagtcggg
      721 tctctctgta tcccggcatt atccatcata taacgccaaa aagtaagcaa cgacaaacac
      781 attacatgta agcagtcatg gcattacatt ctgttaaacc taagtttagc cgatatacac
      841 aacttcaacc tgactttatc gttgtcgata gcgttaatgc gaatgccgtg aagcgagtcc
      901 acggcattgc ctgacgctta tattattgca atttcgcgct gacccagcct ttcacactgg
      961 ctaacgctgc aggtaaggcc gcagcatccg taccaccggc ttgcgccatg tcaggacgtc
     1021 caccaccctt gccgcccacc tgctgagcga ccataccaat cagttcccct gctttcacac
     1081 gatctgtgac gtccttagat acgcctgcaa tcagagaaac cttaccttcg gctaccgttg
     1141 ccagcacgat aattgtcgac cccagctgat tttttaaatc gtcaaccatg gtacgcaaca
     1201 ttttcggctc aacaccgcta agctcgctaa ccaacagctt aacaccatta acatcaattg
     1261 ccttactgga aagatttgcg ctctcctgtg cggcagcttg ttctttaagc tgttgtagtt
     1321 ctttttctaa ctgacgcgta cgttccagta ctgagcgcac tttatccgcc agattattgc
     1381 tatcgccttt cagcagatgc gcgacttcgc ttaagcgatc gctgtctgca tgaacggtgg
     1441 tgatagcgcc ttctccggtt accgcttcga tacgacgaac gcctgcagca gtacccgatt
     1501 cagagatgat gcggaacaga ccaatatcac cagtgcggct ggcgtgagta ccgccacaca
     1561 gctcggtgga gaaatcgccc atgctcagca cgcgtacacg ctcatcatac ttctcgccga
     1621 acagcgccat cgcacctttc gctttcgccg cttcgagatt catgatgttg gtttcgatcg
     1681 gcaaattgcg acgaatctgt gcgttcacca ggtcttcgac cgcacgaatc tcttccggtt
     1741 tcatcgcttc gttgtgtgag aagtcgaagc gcagcacctt gtcgttaacc agtgaacctt
     1801 tctgcgatac atgagtcccc agaacctggc gcagcgcagc gtgcatcagg tgcgttgcgg
     1861 agtgattcag acgaatacgg gcgcgacgag cctcatcaat atcagcctgc acagcgtcgc
     1921 ccactttcag agaacccgca gcaagtttac cgatgtgacc aatcgcctgg ccgtatttct
     1981 gcgtatcttc caccgcgaag gagaagttag cgcctttcag ttcgccttta tcaccaacct
     2041 gaccgccgga ttccgcatag aatggcgttt gatccagcac gaccacagct tcctggcctg
     2101 cattgatggc atcaaccgct ttaccatcaa caaacagcgc ggtcactttg ccgttcagtt
     2161 ccagatggtc atagccttta aattcagatg cactatcaac acggatcatt gcgttgtaat
     2221 cggcaccaaa gccgctggct tcgcgcgcgc gacgacgctg ctcttccatt gctgcttcaa
     2281 aaccagcttc gtcaactttg atgttgcgct cacgacaaac atcagccgtc aggtcaaccg
     2341 ggaagccata ggtgtcgtac agacggaaag ccgtttcacc atccagcgta tcaccagaaa
     2401 gttttgccag ctcttcatcc agcaacgcca gaccgcgctc cagagtacgg gcaaactgct
     2461 cttcttcagt cttcagcacc tgctcaacct gcgcctgctg gcgtttcagg tcttcacccg
     2521 cagagcccat aacgtcgatc agcggaccaa ccagtttgta gaagaaggtt tctttcgcgc
     2581 cgagcatatt gccgtgacgc actgcgcgac gaatgatacg acgcagtaca taaccacggt
     2641 tttcattgga cggcattacg ccatccgcga tcaggaacgc acaagaacga atgtggtcag
     2701 cgattacgcg cagcgattta ttgctcagat cggtcgcgcc agtgactttc gctaccgcct
     2761 ggatcaacgt gcggaacagg tcgatgtcat agttagagtt aacgtgttgc agcaccgcag
     2821 caatacgctc cagacccata ccggtatcta cagacggctt cggcagcggt tccatcgtgc
     2881 catctgcctg gcggttgaac tgcatgaaga cgatgttcca gatctcaatg tagcggtcgc
     2941 cgtcttcttc cggacttccc ggagggccac cccaaatgtg gtcgccgtga tcgtagaaga
     3001 tttcggtgca cgggccgcac ggaccagtgt cacccatctg ccagaagttg tcagatgcgt
     3061 atggcgcacc tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc ccgacttctt
     3121 tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac agacgctctt
     3181 tcggcagggc aaaccatttt tcgctggtca gcagttccca tgcaaactga atggcatcgt
     3241 gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta tggtgacgcg
     3301 cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg cagcgttggg
     3361 aagtggtagc gcgggaataa ttacgcttgt cgagcccaag gaacacatcc ttgaactggt
     3421 tcatcccggc gttggtaaac aacaaagttg ggtcgttatg gggtaccagg gagctgctgg
     3481 caactacctg atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg atctcagcgg
     3541 tgctcttgct cataattatc ctgaaatcaa gctaacgaaa tatcgccacc agctccagcg
     3601 tgtcttaacc gccgggctgg taactgaaaa gtgggaataa gataagtttt cttgactggg
     3661 aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg gatatcttcc
     3721 atcagatagc cacgatagag cagaaaacgc tggatcttaa ctttttctga aaagacagtt
     3781 ggcaaaggtt caccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg
     3841 tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg attcagttcc
     3901 tgacgaatac gtgcaggtcc ataacctttg cggctacggc tggcgataaa gcgcgcaaca
     3961 aatcggctgt catcgagata gccatgttca tggcaccagg caataacgcg ctcgtaatct
     4021 tctgccgtag catcaatctc ttctgggcca tttttgccca taatcggtgc cgcgagttta
     4081 cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac
     4141 aggcgagcat atgccgggcg acgggatgtt gattctgtca tggcatatcc ttacaactta
     4201 aaaaagcaaa agggccgcag atgcgaccca ggttttgctg ataaacagat ttaatagtca
     4261 atgagtatgc agaatgttcc cgcaggccca attcgttaga ggatcgaggt gagagtaatt
     4321 tttcacgcca gtgctggcct gttcccctca ccctaaccct ctccccaaag gggcgagggg
     4381 accgtccgtg cacacattat ctattatcat caataacgaa cgattaaaaa tcttcgttag
     4441 tttctgctac gccttcgctg tcatctacag agaaatccgg cgttgagttc gggttgctca
     4501 gcagcaactc acgtactttc ttctcgatct ctttcgcggt ttccgggtta tctttcagcc
     4561 aggcagttgc attcgcttta ccctgaccga tcttctcacc tttgtagctg taccacgcgc
     4621 ctgctttctc gatcagcttc tcttttacgc ccaggtcaac cagttcgccg tagaagttga
     4681 taccttcgcc gtagaggatc tgaaattcag cctgtttaaa cggtgcagcg attttgttct
     4741 tcaccacttt cacgcgggtt tcgctaccca ccacgttttc gccctctttc accgcgccga
     4801 tacgacggat gtcgagacga acagaggcgt agaatttcag cgcgttacca ccggtagtgg
     4861 tttccgggtt accgaacatc acaccaattt tcatacggat ctggttgatg aagatcagca
     4921 gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat cgcctggctc atcatacgtg
     4981 ccgcaaggcc catgtgagag tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg
     5041 ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg gcgtcacaga
     5101 tttccagtgc ctgctcgccg gtatccggct gggagcacag caggttatcg atatcgacgc
     5161 ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc atcgataaac gcacaggttt
     5221 taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta ccggaagatt
     5281 ccggtccgta gatttcgacg atacggccca tcggcagacc acctgcccca agcgcgatat
     5341 ccagtgaaag cgaaccggta gagatggttt ccacatccat ggaacggtct tcacccaggc
     5401 gcatgatgga gcctttacca aattgtttct caatctggcc cagtgctgcc gccaacgctt
     5461 tctgtttgtt ttcgtcgata gccattttta ctcctgtcat gccgggtaat accggatagt
     5521 caatatgttc tgttgaaaca attatactgt atgctcatac agtatcaagt gttttgtaga
     5581 aattgttgcc acaaggtctg caatacatac gcagtagcct gacgacgcac cgcatcacgg
     5641 tcgccgctga agcattcccg ccgggtaatg ccttcaccgc gggcagtggc aaaagcaaac
     5701 cagacggtgc cgacaggctt ctcttcactg ccgccatccg gcccggcgat accactaata
     5761 gacacggcat aatcagcacg agccgctttc agtgcaccta tcgccatttc caccacgacg
     5821 ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc gtacgccgat catctgcgct
     5881 ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc aggcggagct accggcaata
     5941 tcggtaatca ctttcgctac ccaaccaccg gtacaagact cggcagttgt tacggttgcg
     6001 ccacgggctt tcagcgcctg cccaacctgt tcgcttaact gcatcagttc actgtcagtc
     6061 atcacaacct ctgtcagtca aaaatttatg ccggacaaga tagcactttc gtcaggaaga
     6121 tggtgttggc gttgcaattt tacgaggagg attcagaaaa aagctgatta gccagaggga
     6181 agctcacgcc cccctcttgt aaatagctac tgtactcgcg ccagcgccac ggcttgtcct
     6241 aactgccaga ccgccattgc gtaatgggtg ctgtggttgt aacgggtgat ggtgtagaag
     6301 ttcggcagac cgtaccagta ctggtagccg gtgccaacat ccagacgcag caggctggct
     6361 tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg cggcggcaag ctgcgaaatg
     6421 ctgtacttag ttttgaaacc atttggcaag cctggagcct gaccgtttgc cattatcgcg
     6481 acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt tcgccacgct accgatcgca
     6541 tcaaccggat cccacaggtt gatatgcccg tcgccgctga aatctaccgc atattgtttg
     6601 taagacgacg gcataaactg tccgtagccc atcgccccgg caaaggaacc tttcagattg
     6661 agcggatcgt cctgctcgtc gcgcgccatc agcaggaagg tttccagctc gccagagaaa
     6721 tactccgcgc ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt
     6781 ttccccatca cgcgccccca gcgggtttca acgccgataa tcccgacgat aatttccggc
     6841 ggtacgccat acacctgcca cgcgcgattc aacgcatctt catactgatt ccagaaaacc
     6901 acaccgttct gcacgttgtc cggcgtaata aattttttgc gataacgcag ccatgcgccg
     6961 ttcggacctg atggcggttt caccgatgtg gttggtgcct ggttatccat cagccgcagt
     7021 accgaatcca gacgcttcgc ctgggagaga atttcctgca actgctgacg atcgaaaccg
     7081 tgtttgttca ccattttgtc gatgaactgc tgggcattcg ggttattagc gaaatcgccg
     7141 cccatctgca tcacattgtg ctgcggctca agcaggaagc cgccagacgg cgttccggtg
     7201 gtcgtttcag tctcagtagg ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg
     7261 ggaagcaatg ttacataacg acgcttgaac atgaggggtc catttaacag attcaaccag
     7321 gggcaagtat ggtaaagcat cacgccccgc acaaggaagc ggtagtcact gcccgatacg
     7381 gactttacat aactcaactc attcccctcg ctatactttt actcaaactt tcaaattaaa
     7441 atatttatct ttcattttgc gatcaaaata acacttttaa acctttcaac ctgattagat
     7501 taggttgccg tttggtaata aaacaataaa tcctgaagga gagaacaatg atagaaacca
     7561 ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa gggcggagag gtgtttaccg
     7621 gaatggtgac cggcattctt ccgctgttga ttagcctgct ggttatcatg aacgcactga
     7681 ttaattttat cggtcagcat cgtattgaac gttttgctca acgttgcgcc ggtaaccctg
     7741 tttcccgtta cctactgtta ccgtgcattg gcacgtttgt cttttgcaat ccgatgacct
     7801 taagcctagg tcgctttatg ccggaaaagt acaaacccag ctactacgcg gcggcctctt
     7861 atagctgcca ctcaatgaat ggcctcttcc cccatatcaa ccctggcgaa ctgtttgttt
     7921 atcttggcat tgccagcggt ctgacaacgc tgaacctgcc acttggccca ctggcggtga
     7981 gttatctgct ggttggtctg gtcaccaatt tcttccgcgg ctgggtgacc gatctgacca
     8041 ccgccatttt tgagaaaaag atgggcattc aacttgaaca aaaagttcac ctggcaggag
     8101 caacatcatg acgcgtattc ggatcgaaaa aggaacgggt ggctggggcg gcccgcttga
     8161 gctggaagct acgccgggca aaaaaatcgt ctatatcacc gccggtaccc ggcctgcgat
     8221 tgttgacaaa ctggcacaac ttactggctg gcaggctatt gacggattta aagaaggtga
     8281 acccgcggag gcggaaattg gtgtcgcggt aatcgactgt ggcggcacat tacgctgcgg
     8341 catctatccg aaacgacgta ttcccaccat taatatccac tcgacgggca agtccggtcc
     8401 gctggcgcag tacattgtgg aagatattta tgtctctggc gtaaaagaag aaaacatcac
     8461 tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc cgtgactatg acaccagtaa
     8521 gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg ggaatgggca tggggtccgc
     8581 cgttgccgtg ttgtttcagt ctggtcgtga caccatcgac actgtattaa aaaccattct
     8641 gccgtttatg gcattcgtct cggcgctcat tggcatcatt atggcttctg gccttggtga
     8701 ctggattgcc cacggtcttg ctccgctggc gagccatcca ctgggtctgg tcatgctggc
     8761 gctcatctgc tccttcccac tgctttcacc tttcctcggc ccaggcgcag ttatcgcaca
     8821 ggttatcggc gtattgattg gcgtgcagat tggtctcggc aatattccgc cgcatctggc
     8881 tttaccggca ctgtttgcca tcaacgcgca ggcggcctgc gacttcatcc cggtcggttt
     8941 gtcgctggcg gaagcccgtc aggacacggt tcgcgtcggt gtcccttctg tactggtgag
     9001 ccgcttttta accggcgcgc cgactgtact gatcgcctgg tttgtctccg gttttatcta
     9061 tcaatagagg ctgaaacatg accgttattt atcagaccac catcacccgt atcggcgcga
     9121 gtgccactga cgccctcagc gaccagatgc tcatcacctt tcgtgaaggc gcgccagcgg
     9181 acctcgaaga gtattgcttc attcattgcc acggcgagtt gaaaggtgca ctccatcccg
     9241 gtttgcaatt ttcactcggg cagcatcgct atccggtgac cgctgttggc agcgtggcgg
     9301 aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt cgatggttta aacgaagcgg
     9361 aatttccggg cactgtccat gtggcaggcc ctgtacccga cgatatcgcg ccgggatcgg
     9421 ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca ggttgccgtt gtcatcggtg
     9481 gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc tgccgagggg tatcgcgtcg
     9541 cggttgtcga tattcagagc gacaaagccg caaatgtggc acaagaaatt aacgccgaat
     9601 atggtgaagg tatggcgtac ggttttggtg ctgacgccac tagcgagcaa agcgttctgg
     9661 cgctctctcg tggggtagat gaaatctttg gtcgcgtgga tttgctggtc tatagcgccg
     9721 gaatagcaaa agcagccttt atcagcgact tccagctcgg cgattttgac cgttcgctac
     9781 aagtgaatct ggtgggttat ttcctgtgtg cccgtgaatt ttcgcgtttg atgatccgcg
     9841 acgggattca gggtcgcatt attcagatca actcgaaatc cggcaaagtg ggcagcaaac
     9901 acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg gctgactcaa tcactggcgc
     9961 tggatctggc ggagtacggc attacggtgc attcactgat gctcggtaac ctgctgaaat
    10021 cgccgatgtt ccagtcactg ttgccacaat acgcgaccaa gctgggtatc aaaccggatc
    10081 aagtcgagca gtattacatc gacaaagtac cgctcaaacg cggctgcgat tatcaagatg
    10141 tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc gtactgcacc ggacagtcga
    10201 tcaatgtcac cggcggtcag gtgatgttct gatcaacagc ggagatccgt taaggatctc
    10261 cgtgagacta tagaatgcct gatgcgctac gctcatcagg catacaggac ttccgccact
    10321 acattaagga aaagttatgg tatccgcact catcaccgtc gccgttatcg cctggtgtgc
    10381 gcaactggcc ttaggcggct ggcaaatttc tcgttttaac cgtgccttcg acacactatg
    10441 ccagcaaggg cgggttggcg tgggccgttc cagcgggcac tttaaaccgc gggtcgtggt
    10501 cgccatcgcg ctggacgatc agcagtgcat cgtcgacacc ttgtttatga aaggactgac
    10561 tgtcttcgcc cgaccgcaaa aaattcccgc aattaccggc atgcatgcgg gtgatttaca
    10621 gcccgatgtg atctttcccc atgatccact atcacagaat gctctatcat tggcgcttaa
    10681 actgaaacgt ggataatttc gttgtgaatg ttacttgctt gcgaagttat cattttgaaa
    10741 cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt caggccgcca ttctggagta
    10801 tctgcaaaag cagggtaaat gctcggttga agaattggcg caatactttg acaccacagg
    10861 cacaaccatt cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca ttcgtactta
    10921 tggcggagtg gtgttgaata aagaggaatc cgatccgcct atcgatcata aaacgctcat
    10981 caacacccac aagaaagagc tgattgcaga agctgccgtt agttttatcc atgatggcga
    11041 ttcgatcatt cttgatgctg gcagtaccgt tttgcagatg gttcccctgc tctcgcgctt
    11101 taataacatc acggtgatga ccaacagcct gcatatcgtc aatgcgctat ccgaactgga
    11161 taacgaacaa actatcctga tgccaggcgg aacatttcgc aaaaaatcgg cctcatttca
    11221 cgggcaactg gcagagaatg ccttcgagca tttcaccttc gataaattgt ttatgggcac
    11281 cgacggcatc gatctcaatg cgggcgtaac cacgttcaac gaggtttata ccgtcagtaa
    11341 ggcaatgtgc aatgccgcgc gcgaagtgat tttgatggcg gactcatcaa agtttggccg
    11401 taaaagcccc aacgtagttt gcagtcttga aagcgtcgat aagctaatta ccgacgcagg
    11461 tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg atcgatgtga tcataaccgg
    11521 agagagcaat gagtgaagca ctactgaacg cgggacgtca gacgttaatg ctggagttgc
    11581 aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt tgttcgcgcc gccaatatca
    11641 tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc cacattggta
    11701 agaaaatcgc cgcaacactt gccagtaccg gcacaccggc tttttttgtc catccggcag
    11761 aagcactgca cggcgatctg gggatgattg aaagccgcga tgtgatgctg tttatctctt
    11821 actccggtgg cgcgaaggaa ctggatctga ttattccgcg tctggaagat aaatctatcg
    11881 cgctgctggc gatgaccggc aaaccgacgt caccgctggg cctggcggcg aaagcggtgc
    11941 tggatatctc cgtagaacgc gaagcctgcc cgatgcacct tgcgccgacc tccagcaccg
    12001 tcaataccct gatgatgggt gacgcgctgg cgatggcggt catgcaggcg cgcggtttta
    12061 atgaagaaga ttttgcccgc tcccacccag ctggggcact gggcgctcgc ttgctgaata
    12121 aagtgcatca tctgatgcgt cgtgatgacg ctatcccaca ggtagcgtta accgccagcg
    12181 tgatggatgc gatgctggaa ctcagcagca ccggtctggg gctggtggcg gtatgtgacg
    12241 ctcaacaaca ggtacaaggc gtctttaccg acggcgattt acgtcgctgg ctggtaggcg
    12301 gcggcgcact caccacgcca gttaatgaag cgatgacgac aggcggcacc acattacagg
    12361 cacaaagtcg cgccatcgac gccaaagaag ttctgatgaa acgcaaaatc actgccgcac
    12421 cagtggtaga tgaaaacggc aaactcaccg gcgcaattaa cctgcaggat ttctatcagg
    12481 ccgggattat ttaatccttc aatcccagac gtttcgccag ccgatgcagg ttggcgacgt
    12541 cagtttccag cattcgcgca caggctgccc agttgtgatg attttgtgcc agtgcctggc
    12601 gaatagtttc acgctggaac gcttctgtcg cttcacgcag gttttgctta acaacgggca
    12661 ccgccgccgc ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt tgcgcctcaa
    12721 gaatcacttc atcgccgttg cgggtggctc tcgccagcac taccgcccga tgaatagcat
    12781 gttccagttc gcgcacgttt cccggaaaac tgtagtgttg cagtaaattt cgcgccccgg
    12841 cacttaatac cacgcgggag agcccctgcc gcaaacgaca ctgctcgcag aaataccccg
    12901 ccagcagaat gacatcatcg ccccgctcac gcagcggcgg caccgaaagt ggaaacacgc
    12961 tcaggcgatg aaacaaatcg gcgcggaatc gccctgccag cacctcttcg cgtaagtcgc
    13021 ggttagtcgc cgccagcacg cgcacatcga cccgcaaact acggtcattg ccaacgcgct
    13081 gaatatcgcc atactgcaac accctcagca gcttggcctg caatgccaac gacaactcgc
    13141 cgatctcatc gagaaacaga gtaccgttat ccgccatttc aaacttcccg ctgcggttac
    13201 tgatagcgcc agtaaacgct cctttcacat gcccgaacaa ctcactttcc gccacacttt
    13261 ccggcagtgc ggcacagttg agatagacca gcggattcac cgcccgtggc gaggcttcat
    13321 gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc accgctgatc agaacgttga
    13381 gatcggacgc cgccacaatc tcaatctctt ttttcagttg cgtcatgcca ggggacaagc
    13441 caatcatctg cgtctgtttc accgcttcaa acggcgtggc atcgcctggc agcatattct
    13501 ggctttccag ttgttcaatc agcaacgcat tgcttaacgc tcccgccgcc agcgcggcaa
    13561 tcagccgtaa ctcttcgtcg ctgaaaacat cgaactgatc gggctgcatc ccgtcgagcg
    13621 tcagtgcgcc gatcaggttt tgtccgacaa acaatggcag accaacgcag gcgtgaacct
    13681 tcagactctc ctgcccagga atcaaaccgt catagggatc gggcaattcg ctgtctgcgg
    13741 gaaagcgcac cacatccccg gcgcgggcaa tcgcttccag ccgtggatgc ccttccagcg
    13801 caaagcgtct accgagtaca tcctttgcca gaccgtcgat ggcaagcgga ataaactgcc
    13861 gcgaatcgta acgtagcaac gcagacgcat cgcactccag cacctgacgt agcgtggtga
    13921 tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg caattcgatg gcgatattcg
    13981 ccagcacatc aacggaaaaa ctcatctttg cctcactgtc aatttgacta tagatattgt
    14041 catatcgacc atttgattga tagtcatttt gactactcat taatgagcat aattttattt
    14101 atagagtaaa aacaatcaga taagaaactg gcacgcaatc tgcaattagc aagacatctt
    14161 tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa ataacattca
    14221 ttgggtgggt caacgtgact gggaagtgcg tgattttcac ggcacggaat ataaaacgct
    14281 gcgcggcagc agctacaaca gctacctcat ccgcgaagaa aaaaacgtgc tgatcgacac
    14341 cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa tcgatctggc
    14401 ggatatcgat tacatcgtga ttaaccatgc agaagaggac cacgccgggg cgctgaccga
    14461 actgatggcg caaattcccg atacgccgat ctactgtact gccaacgcta tcgactcgat
    14521 aaatggtcat caccatcatc cggagtggaa ttttaatgtg gtgaaaactg gcgacacgct
    14581 ggatatcggc aacggcaaac agctcatttt tgtcgaaaca ccaatgctgc actggccgga
    14641 cagcatgatg acttacctga caggcgacgc ggtgctgttc agtaacgatg ctttcggtca
    14701 gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc ttttcgagca
    14761 gtgccagcgt tactacgcca atatcctgac gccgttcagc cgcctggtaa cgccgaaaat
    14821 taccgagatc ctgggcttta acttaccagt cgatatgata gcgacctccc acggcgtggt
    14881 ctggcgcgat aacccgacgc aaattgtcga gctgtacctg aagtgggccg ctgattatca
    14941 ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca tgatggctga
    15001 cgctatcgcc cagggaattg cggaaaccga cccacgcgtg gcggtgaaaa ttttcaacgt
    15061 cgcccgaagc gataaaaacg aaatcctgac caatgtcttc cgctcaaaag gcgtgctggt
    15121 cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccgggctgg tggaggagat
    15181 gaccggatta cgcttccgta acaaacgcgc cagtgctttc ggatctcacg gctggagcgg
    15241 cggtgcggtg gatcgccttt ccacgcgcct gcaggatgcg ggtttcgaaa tgtcgctcag
    15301 cctgaaagcc aaatggcgac cagaccagga cgctctggag ttatgccgtg aacacggtcg
    15361 cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga atacggtagt
    15421 taaagaagaa acctctgcca cgacgacggc tgacctcggc ccacgaatgc agtgcagcgt
    15481 ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg cgccaggaac
    15541 gccgtggagt gaagtcccgg ataacttcct ctgcccggaa tgctccctcg gcaaagacgt
    15601 ctttgacgaa ctggcatcgg aggcaaaatg agcaacggca ttgtgatcat cggttcgggc
    15661 ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccactat tccattaacc
    15721 ctgattgccg ccgacagcat agatgagtac aacaaacccg acctcagcca tgttatcagt
    15781 caggggcaac gtgccgacga ccttacccgc cagacggcgg gtgaatttgc cgggcagttt
    15841 aatctgcgcc tgtttccgca cacctgggtg acggatatcg atgccgaagc ccatgtggtg
    15901 aaaagccaga ataatcagtg gcaatacgac aagttagtgc tggcaaccgg tgccagcgcc
    15961 tttgtcccgc ctgtgcctgg gcgtgagtta atgctgacgt taaatagtca gcaagagtat
    16021 cgcgcctgtg aaacgcaact gcgggatgcc cgacgcgtgt tgattgtcgg cggtggtttg
    16081 attggtagcg aactggcgat ggatttttgt cgtgcaggca aagcggtcac gctaatcgac
    16141 aacgctgcca gtattctggc gtcattaatg ccaccagaag taagcagccg cttgcagcat
    16201 cggttgacgg agatgggcgt tcatctgctg ttgaaatctc agttgcaggg gctggaaaaa
    16261 acggattctg gcattctggc aacgctggac caccagcgta gtatcgaagt ggatgcggta
    16321 attgctgcca ccggactgcg cccggaaaca gccctggcac gacgcgccgg gctgacgatt
    16381 aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgccgatat ttacgcgctg
    16441 ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc tgcagccgat tcaacttagc
    16501 gggatggtgc tggcaaaaaa tcttctcggc aataacacgc cgctgaaact accggcgatg
    16561 ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac ccagcgtcag
    16621 gatttacgct ggcagattaa taccgaacgc cagggaatgg tggcgcgcgg cgttgacgat
    16681 gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc atttgtattg
    16741 ttgaaaacgt tgccggtgta ggtgggctac tgtgcctaaa atgtcggatg cgacgctggc
    16801 gcgtcttatc cgacctacgg ggacgcatgt gtaggccgga taaggcattt acgccgcatc
    16861 cggcaagggt gtccaaatgc aacacgcttt atccgttctg gacttcaccc gctaaccaac
    16921 gcgccgcagc aataaccccc tgccccagag acaaaccgcc atcacccgcc ggtaaactct
    16981 gtggaaagag caatgtgaaa tcagcgagat aatgcgccag acgtgcacgc agcaaacggt
    17041 tatgaataac cccgccgcta aataccagcg tagtgatacc acgcatcgtg gcctgctcac
    17101 gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg cgttgattaa
    17161 ccggtgcctg ccagttcagc cactgctgcc agaaagtggc gagatccagt tgattgtcca
    17221 cccgcggcat cgtcaccgga tgcgtcactc cgtcgcatga ggctgcgagc gcctccagag
    17281 cacaagccgc ttcaccttca taacttaacg tggctggcgc acagcccagt gccgccgcca
    17341 ctgcatcgaa caaccgccca cacgatgatg ccagcggcgc gttaattcca cgctcaatgg
    17401 cccgcgccag tacgctccag ttttgctgtt gcacacttgc cgtttcaggg taattctgcc
    17461 actccggcac aaagcgcagg cactgcgcca gcaggtttcg ccacggctgc ttcgccgcca
    17521 aatcgccacc cggaagcgcc actgcaggca agccgcccag gtgctcacat tcgcgatagt
    17581 tcacccgcag gcactcgccg ccccacaaag cgccattctc ccccataccg ataccgtcga
    17641 gcgtcaaagc aatgacatca ccgccatcca gcggccactg atgctctgcc agacacgccg
    17701 ctgcatgggc atgatgatgc agtaccgttt gcgtcggcag attcatttcg ctcgcccact
    17761 ggctggagac atagcccgga tgcacatcat gcacaatgta ttgcggagtg aaatcgtaga
    17821 tgttttgcat caggcgtaac gcttcgcgcc actgcatctg gatgccatcg tcacttaaat
    17881 cgcccagatg ctgactcaac accacttgtt caccgcgcac caggcagaag gtatttttca
    17941 gatccgcgcc aagacacagc acaggcggaa catttttaaa gcccggaggc aaaaccagcg
    18001 catccggcac atacccccgc gaacggcgca gcatttcgcc gctttcgcgc accactgaat
    18061 catccatccg ctgcacgatg tcgcggttat gtagcaagaa tccgtcggca atgccctgca
    18121 aatccgccag cgcctgttcg ttgctgatag ctggtggttt accgctcagg ttgccggagg
    18181 tcatcaccag cgggcattgc agttcctgta acagcaaatg ctggagcggg ttcgcaggca
    18241 acatcacccc gacttcgtta aggccagggg cgatatcatc acaaagctca ggtacgtatt
    18301 ttttatccac cagcacaatc ggcgcagcgg gcgtggtaag caactggcgc gcagcgtctg
    18361 gtaaactttc tgccactggc aacatgactg ccagcggttt cgccgggcga tgtttgcgct
    18421 cccgcagtgt cgccaccgcg ttactgttac gtgcatcgca ggcaagatga aatccgccaa
    18481 tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagctgcc tgtaatgccg
    18541 cctcttgttc tgcatgttca ccatgactta cccattcaag atgcgggcca cactccgggc
    18601 aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc gcggtactct ttgtcacagg
    18661 ccggacatag cggaaacgcc gccatcacgg taaacgggcg gtcgtaaggc atcgcgcgaa
    18721 taatggtgaa acgcgggccg cagtgggtac agttgataaa cggataacga taacgccgct
    18781 cgcctggggt attcatttcg gcaaggcaag cagggcaagt ggcggcatcc gggacaattt
    18841 gcgtattcat ggtgccgccg gtgctctggc ggatggtgaa ctcggtgggc agttgtgacc
    18901 agataaacgg ctcacgctcg acgctatcaa tacgcgccag tggcggacag tgctgatgca
    18961 attgaacaag aaacgtttcc ggatcttcca gcagccggac ttctacgcca tcgccgtcat
    19021 tacatacatc gccgtgaaga tttaattgct gtgccagctg ccagacaaac ggacgaaaac
    19081 cgacgccctg cactttgcca cgaatacgca gttggacacc gcaaaatgtg ttttttgcca
    19141 ttgagttatt cccgccatca tgaattgcgt aacccgccct gccggacacg acagcgtcgt
    19201 atccggcagt ctcaggtcgg cgataccgcc cgctccgtat tctacgaata tttccgggaa
    19261 ttcctttgat gccagaacag ttctgtaaga tttttagaac atcagcgccg tacggcggcg
    19321 tttttctgcg ctcagttgtt caagtttatt acgatcgaca caaatcagcg catgggtcgg
    19381 gcaagccgcc atacacgccg ggccgtcttc acgatggttg cacaggtcgc atttattggc
    19441 ttcggctttg tcagcccgta cattcagacc cgcgccgctg ttgcgaatca caggacgtac
    19501 caccacttcc atcgcgccat acgggcaagc caccacgcag gttttgcaac caatgcaacg
    19561 ttcctgcatc acatgaacaa accctttatc acagctgata gcaccattcg ggcagacgtt
    19621 agcgcacggt gcatcttcac actgacggca aactgtcgcc gtggaaatgt tcacaccttt
    19681 aatgacatgg atacgcggta aaaatgtttc cggggtcagc gatgcacagt cctgattttc
    19741 ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt tactcgcgtc
    19801 agcaatgatg aaacggttca tcaaattctc cagcaatgac agtt
//
LOCUS       DADLTF010000072        19787 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.072, whole genome shotgun sequence.
ACCESSION   DADLTF010000072 DADLTF010000000
VERSION     DADLTF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.072"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            10..276
                     /locus_tag="JN749_002365"
     CDS             10..276
                     /locus_tag="JN749_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7368752.1"
                     /translation="MNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVV
                     TLIDDNIREEILRPEWIAGETGMSVRSLYRMFADIRFGSRAIYS"
     gene            251..442
                     /locus_tag="JN749_002366"
     CDS             251..442
                     /locus_tag="JN749_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7368753.1"
                     /translation="MVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFS
                     TVFKQRFGMTPGEYRRKFR"
     gene            complement(614..943)
                     /locus_tag="JN749_002367"
     CDS             complement(614..943)
                     /locus_tag="JN749_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HAY7368754.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(948..1133)
                     /locus_tag="JN749_002368"
     CDS             complement(948..1133)
                     /locus_tag="JN749_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HAY7368755.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1130..3769)
                     /locus_tag="JN749_002369"
     CDS             complement(1130..3769)
                     /locus_tag="JN749_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HAY7368756.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3977..4966
                     /gene="ldhA"
                     /locus_tag="JN749_002370"
     CDS             3977..4966
                     /gene="ldhA"
                     /locus_tag="JN749_002370"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7368757.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5077..5424
                     /gene="hslJ"
                     /locus_tag="JN749_002371"
     CDS             5077..5424
                     /gene="hslJ"
                     /locus_tag="JN749_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HAY7368758.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEISFGEKMMISGSMCNR
                     FSGEGKLSNGELTAKGLAMTRMMCANPQLNELDNTISEMLKEGAQVDLTANQLTLATA
                     KQTLTYKLADLMN"
     gene            complement(5421..5687)
                     /locus_tag="JN749_002372"
     CDS             complement(5421..5687)
                     /locus_tag="JN749_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HAY7368759.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5961..9485
                     /gene="nifJ"
                     /locus_tag="JN749_002373"
     CDS             5961..9485
                     /gene="nifJ"
                     /locus_tag="JN749_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAY7368760.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9852..10981
                     /gene="ompN"
                     /locus_tag="JN749_002374"
                     /pseudo
     CDS             9852..10981
                     /gene="ompN"
                     /locus_tag="JN749_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11122..11556
                     /gene="uspF"
                     /locus_tag="JN749_002375"
     CDS             11122..11556
                     /gene="uspF"
                     /locus_tag="JN749_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HAY7368761.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11732..12667
                     /gene="ttcA"
                     /locus_tag="JN749_002376"
     CDS             11732..12667
                     /gene="ttcA"
                     /locus_tag="JN749_002376"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HAY7368762.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="JN749_002377"
     CDS             complement(12796..14163)
                     /gene="dbpA"
                     /locus_tag="JN749_002377"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HAY7368763.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTLALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="JN749_002378"
     CDS             complement(14641..15624)
                     /gene="zntB"
                     /locus_tag="JN749_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HAY7368764.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            15879..17111
                     /gene="dgcM"
                     /locus_tag="JN749_002379"
                     /pseudo
     CDS             15879..17111
                     /gene="dgcM"
                     /locus_tag="JN749_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="JN749_002380"
     CDS             complement(17132..17695)
                     /gene="smrA"
                     /locus_tag="JN749_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HAY7368765.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17949..19403)
                     /locus_tag="JN749_002381"
     CDS             complement(17949..19403)
                     /locus_tag="JN749_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7368766.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19400..>19787)
                     /locus_tag="JN749_002382"
     CDS             complement(19400..>19787)
                     /locus_tag="JN749_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7368767.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5203 a         5011 c         4665 g         4908 t
ORIGIN      
        1 caggagagca tgaataatcc ggcactttct gaaacagaaa gtgaagctgc gctacaggcg
       61 atggtgtgtc tgcttcgccc agtacttcat cagcgggaat ctgtccaacc tcgccgtgaa
      121 cgtcagtttc aaaaagtggt tacgttgata gacgataata ttcgcgaaga gatattacgc
      181 ccggagtgga tagccggaga gacaggtatg tcagtacgta gtttgtaccg aatgtttgcc
      241 gatataaggt ttggtagtcg cgcaatatat tcgtaaccgt cgtctcgatt tttgtgcaga
      301 tgcgattcgc catgccgcag atgatgaaaa actggcaggc atcggctttc attggggatt
      361 ttctgaccag agtcatttct caacggtatt taagcaacgc tttgggatga cgccaggcga
      421 gtatcgccgt aaattccgct aactaattgg cgttgcatgt actgcaacgc caattagtta
      481 aaacagatat ttgactatgc atttttcagg gcgaaaggca atttgacagg agtttttgaa
      541 tattcacctt gtttcaggaa aatagatcaa tcgctttctc ctgtagcgtt atcccgatat
      601 tgcggttttg ctattaaaac tttcgcaccc atttaccgtt tatcccttgt acatattccc
      661 ccggtttggc ccgagctacg agcttttgcc cggcgagttt cgcgatatca tctacagaca
      721 cattattttg ctttgcccgt tgttggtaac tcgctttacg ttcggcatta atttcggtta
      781 ctaatttctc tgtttcagca tccgttttca gcgcaaccag ataaccataa aatgtttcac
      841 ctacccgccc ctgagttctg gcttcatcca gagtcaatgc cattacattg ctgcttacca
      901 gcccaacaag aaacgcacaa agaagtaatg ttttcttcat catcgcctca gaaaagatcg
      961 ctacgagttt caagcagctc ttcgacatct ttgtctgcct tgatgatgat ctcatgctca
     1021 attttaacgt tcatattgat agtgatcggt tccttaggtg cagcgacttc aatgcgaggc
     1081 gtacagccaa ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgttttt
     1141 cctcacactc ttttccttgc ggacagtcat ttcccggcag acgtgcgttc tgctccaacc
     1201 atgcctggag attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat
     1261 gatggtaatt aaggtttacc gtaccgcttt taccatcaac gcgactggta cctgtaatgt
     1321 tggcctgcat ggttaataaa ccgagattat ctaagttaat ttttgtcgat gaccggctaa
     1381 tttccatata gcgcaaccag ttaattgctg aacccgcagt catattgtct ttcaccaccg
     1441 catctgcggt atctttatcc agccgcaagg tcattggccc gctatttgcc agccaaccat
     1501 ctttcactat ccatttttcg ttgtttagcc ataacggcaa tgcaccacta aatgccccgg
     1561 acatggcgaa ttgtttcgga ttgacggcgc taaccagttc gctggatgag agattattca
     1621 gccgcaacag cgccgggtca tgttgcggca tacgtaattg tttcatcagc acgttaccgc
     1681 ctaacacatc gacgctaaca tcagtcaaca gcaagggttc ttcttcagtc cacggataac
     1741 gcccttgcaa atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc
     1801 gcaacgtaac ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca
     1861 ggacaaaatc gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaaca
     1921 cgccgtgccc tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca acctgagcat
     1981 acagttcacc atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc tggaatacag
     2041 tcagtgattg ttttggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat
     2101 ttacccgaac cgggccaatc tcaccaacat gtaaatcgcc tttaaagagg aaataggtcg
     2161 gatcgcgccc atcgacgcta aattttaagg ttgatggcgg taacacactg ccgccagtga
     2221 acagcgtttg cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc tgtgcgtcac
     2281 gtacccagac gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt
     2341 attgtaactg gtcgaaaccg gtggagagat cggtcagcgt aatcgtgctg tcatgccact
     2401 caccttttcc tgcgacatcc caggtggcat tcatcggtgt aaaactccct tttccccagt
     2461 agcgccactg ccagcggcca gcgtcaggga gaaaatcatt cgccagccca tccatatgca
     2521 gcacgaaatc gcccagttca ttttcatgcg cctgcaaaat ggcctgcaaa cgtccgtcaa
     2581 caccacgttg ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg
     2641 aatcgatgac tcttcccttt gaacgaagta acgcgcctgg ctcaaaggtc agcgttgggt
     2701 cagacagact tccacttagc tgcgcaggta aacgggcata taaaatgagg tccgcctgtt
     2761 tcgcttcacc ggtcagctgc agaggcagtt gactgttatc catgcttaat tttcctgggc
     2821 caaaattaag caccgcgttg cccttacccg cttgcccctg ggtcagcaca ctcagtcgtc
     2881 cgctgaccag agcgttctca agccctgcct gccagttgtc aactttgaca cccagtcggc
     2941 cactcaaagg aaaacctgca tacagccagc tccagcgacc atcgcttacg gtcaattgtt
     3001 gacgagtaat ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacga
     3061 tcaattgccc gctattttcc tgccagtcca gctcggcatc caccagtgac ggttcctgcg
     3121 gcaagtttaa cgtcgcagta gcatgcccac ttacaggaag tccatccggc acgagcggca
     3181 tagtaaattc ccccaccagt ttaaccggcg gctgattttc aaacgcgacg acatccagtt
     3241 cgctgactgt aagttgttgc cctttcagct ggccttgaaa tttaactttt tcgccctgat
     3301 aacgcagttg ctggatatca gaggttaatg cgagagagag ttttccctgc cattcctgcc
     3361 agggagaaaa aatcagttta tcgatattga tccaggtgtt aggcagcatg gactgccact
     3421 gcgcgagggt ttttggagca gctggcgatt gctccgtctg cggcaatttc gccagacaag
     3481 cagaatcaag ttctaccgtg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg
     3541 cgttggtgat atgcgcaagc tgacaatctc ccaccagata acggagatcg ggaatgatta
     3601 aacctttacg cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc
     3661 agatgtccgc cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa
     3721 gaataatcag taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat
     3781 atcctgattt cgcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc
     3841 agttgaaggt tgcgcctaca ctaagcatag ttgttgatga atttttcaat atcgccatgg
     3901 ctttcaatta tatttgaaat tttgtaaaat atttttagca gcttaaatgt gattcaacat
     3961 cactggagaa agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta
     4021 cctgcaacag gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac
     4081 ggaaaaaacc gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga
     4141 cggcagccgc ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg
     4201 ctgtgccggt ttcaataacg tcgaccttga tgcggcaaaa gaactggggc tgaaagtagt
     4261 ccgtgttcca gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac
     4321 gctgaaccgc cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga
     4381 aggtctgacc ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat
     4441 cggtgtggcg atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc
     4501 gtatccaagt gcagcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt
     4561 ctctgaatca gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt
     4621 gaacgaagcc gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg
     4681 tgcattgatt gattctcagg cggcaattga agcgctgaaa aatcagaaaa ttggttcgtt
     4741 gggtatggac gtctatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt
     4801 gatccaggat gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccgggca
     4861 ccaggcattc ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt
     4921 aagcaatctg gaaaaaggtg aaacctgccc gaacgaactg gtttaatctt gccgctcccc
     4981 tgcaatccag gggagctgat tcagataatc cccaatgacc tttcattctc tattcttaaa
     5041 atagtcctga gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt
     5101 gcgctaagcc tgctgatggc gggatgtgta agtaatgaca aaattgctgt tacgccagaa
     5161 atcagctttg gtgaaaaaat gatgatttcc ggcagcatgt gtaaccgctt tagcggtgaa
     5221 ggcaaactgt ctaatggtga actgacagcc aaagggctgg caatgacccg tatgatgtgc
     5281 gctaacccgc agcttaatga actcgataac accattagcg aaatgctgaa agaaggtgca
     5341 caagtggatc tgaccgcgaa ccagttaacg ctggcgaccg cgaaacagac attaacttat
     5401 aagctggcgg atttaatgaa ttaatagctg ccacagctcc cggcggcaag tgactgttcg
     5461 ctacagcgtt tgccgttggg taatgcacac atcccaatcg ccgtaccatc cagttgacgg
     5521 gcaacagaaa gcgaaccgcc gatcattgca caatttgctt ctccactact ggacatcgac
     5581 gcttttaaac ttggcgctac gtgcgccgca gtcgcctgct gaacaggctc actactacac
     5641 gccgacaaca ataaagcggc acaccctacc caaaacgctg ctcgcatctc ttcttcctct
     5701 gatcttcaag ccaaacgaca ccgccataaa taataggcag cacagagggc ggcgtcgaga
     5761 gctgtcctgc gcgttgcccc gcatttttac ttttttatgg ctattttttt gccctctgtt
     5821 tgatcaaaac attcattact ctgatgtggg ggacacaaaa gcgaaaatgc agaagaaagc
     5881 catttgctaa aattgaagga ttactactgg gcgcgcagca atttcgtgcg cccctcattt
     5941 gcgcaatgta agggtgtcat atgattacta ttgacggtaa tggcgcggtt gcttcggtgg
     6001 cgtttcgcac cagtgaagtt atcgccatct accctattac ccccagttcc acgatggcag
     6061 aacaggctga tgcctgggcc ggaaacggct tgaagaacgt ttggggagac acaccacgcg
     6121 tggttgaaat gcagtcggaa gcgggtgcta tcgctaccgt gcatggcgct ttgcagacgg
     6181 gtgccctttc aacatcgttt acgtcatcgc agggtttgct gctgatgatc ccgacgctgt
     6241 acaaactggc aggcgaactg acgccatttg tcctgcatgt agcggcacgt accgttgcca
     6301 cacatgcact ttctattttt ggcgatcatt ccgacgttat ggcggtgcgc cagacgggtt
     6361 gcgcgatgtt gtgtgcggct aacgtccagg aagcgcaaga ctttgctctc atttcacaca
     6421 tcgcaacgct gaagagccgc gtgccattta ttcatttctt tgatggtttt cgcacgtccc
     6481 acgaaatcaa taaaattgtc ccgctggccg atgacactat tcttgatctc atgccgcagg
     6541 ccgaaattga tgctcatcgc gcccgggcgc tcaacccgga acatccggtg atccgcggta
     6601 cgtccgccaa tcctgacact tatttccagt ctcgcgaagc caccaacccc tggtacaacg
     6661 cggtctatga ccatgttgaa caggcgatga atgatttcgc tgccgcgaca ggtcgtcagt
     6721 atcagccgtt tgagtattac gggcatccgc aagcggaacg ggtgattatc ctgatgggct
     6781 ctgccattgg cacctgtgaa gaagtggttg atgaattgct aacccgtggc gaaaaagttg
     6841 gcgtgctgaa agttcgtctg taccgtccct tctccgccaa acacttgctg caagctctgc
     6901 cgggatccgt acgcagcgtg gcggtactgg acagaaccaa agaacccggt gcccaggcag
     6961 aaccgctcta tctggatgtg atgaccgcac tggcaggagc ctttaataat ggtgagcgcg
     7021 aaaccctgcc ccgtgtcatt ggtgggcgct atggtctttc atccaaagaa tttggcccgg
     7081 actgtgtact ggcggtattt gccgagctca acgcggctaa acctaaagca cgctttacgg
     7141 ttggtattta cgatgatgtg accaatctgt cactgccgtt gccggaaaac actctgccaa
     7201 actcggcgaa acttgaagcc ttgttttatg gccttggtag tgatggcagc gtttccgcaa
     7261 ccaaaaacaa tatcaagatt atcggtaatt ccacgccgtg gtacgcacag ggctattttg
     7321 tttacgactc caaaaaagcg ggcggcctga cggtttctca ccttcgcgtg agcgaacagc
     7381 cgattcgttc cgcttatctc atttcccagg ctgattttgt tggctgccac cagttgcagt
     7441 ttatcgataa atatcagatg gctgagcgtt taaaacctag cggcattttc ctgcttaata
     7501 cgccgtacag cgcagatgaa gtgtggtcgc gcttgccgca ggaagttcag gccgtgttaa
     7561 accagaaaaa agcgcgcttc tatgtgatta acgcggcgaa aatcgcccgc gaatgtggcc
     7621 tggcggcccg tattaatacc gtcatgcaga tggctttttt ccatctgacg caaattctgc
     7681 ctggcgatag cgccctcgca gaattgcagg gtgcgattgc caaaagttac agtagcaaag
     7741 gtcaggatct ggtggagcgc aactggcagg ctctggcgct ggcgcgtgaa tccgtagaag
     7801 aagttccgtt gcaaccggta aatccgcaca gcgccaatcg accgccagtg gtttccgatg
     7861 ccgcccctga tttcgtgaaa accgtaaccg cggcgatgct cgccgggctt ggtgatgccc
     7921 tccccgtttc ggcgctgccg ccagacggca cctggccgat gggcaccact cgctgggaaa
     7981 aacgcaatat cgccgaagag atccccatct ggaaagagga actctgtacc caatgtaacc
     8041 actgcgtcgc cgcttgccca cactcagcta ttcgcgcaaa agtggtaccg cctgaagcga
     8101 tggaaaacgc ccctgccagc ctgcattcgc tagatgtgaa atcgcgtgat atgcgcgggc
     8161 agaaatatgt cttgcaggtg gcaccggaag attgcaccgg ttgtaacctg tgcgtcgaag
     8221 tttgcccggc gaaagaccgt cagaatccag agattaaagc catcaatatg atgtctcgcc
     8281 aggaacatgt cgaagaagag aaaatcaatt acgatttctt cctcaacctg ccagaaatcg
     8341 accgtagcaa actggaacgt attgatattc gtacatcgca gctgattaca ccgctgtttg
     8401 aatattcagg tgcttgcccc ggttgtggcg agacgccgta tattaaattg ctgactcagc
     8461 tctatggtga ccggatgttg atcgctaacg ccaccggctg ttcttcaatt tatggcggta
     8521 acctgccctc tacaccgtat accaccgatg ccaacggtcg tgggccggca tgggcgaact
     8581 ctctatttga agataatgcc gaatttggcc ttggtttccg cctgacggtc gatcaacacc
     8641 gtgtccgcgt gctgcgtctg ctggatcaat ttgccgataa aatcccggcg gaattactga
     8701 cggcgttgaa atcagacgct acgccagagg ttcgtcgaga acaggtcgca gctttacgcc
     8761 agcaactcaa cgatgttgcc gaagcacatg aactgctgcg tgacgcagat gcactggtgg
     8821 aaaaatcaat ctggctgatt ggtggcgacg gctgggctta cgatatcggc tttggcggtc
     8881 tggatcatgt attgagtttg acggaaaacg tcaacattct ggtgctggat acgcaatgct
     8941 attccaacac cggtggtcag gcgtcgaaag cgacaccgct gggtgcagta actaagttcg
     9001 gcgagcacgg caaacgtaaa gcgcgtaaag atcttggcgt cagtatgatg atgtacggtc
     9061 atgtttatgt ggcgcagatt tctctcggcg cgcagctgaa ccagacggtg aaagcgattc
     9121 aggaagcgga agcgtatccg ggaccatcgc tgatcattgc ttatagcccg tgtgaagagc
     9181 atggttacga tctggctctc agccacgacc agatgcgcca actcactgct accggcttct
     9241 ggccgctata tcgctttgat ccgcgtcgtg ccgatgaagg caaactgccg ctggcgctgg
     9301 attcacgccc gccgtcagaa gcactggaag aaacgttact tcacgagcag cgtttccgtc
     9361 gtctgaattc gcagcagcca gaagtggcag aacagttatg gaaagatgct gcagctgatt
     9421 tgcaaaaacg ctatgacttc ctagcacaaa tggccggaaa agcggaaaaa agcaacaccg
     9481 attaaatgct ctggataagg attatccaat tctaaaaaaa aagcccggac gactgttcgg
     9541 gcttgtcttt ttatatgttg gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa
     9601 tctgtgacaa taaaggcata taacccgcgc agaataacgt atagaaaatt aaaagtataa
     9661 tttttatttt gtatgtatta tcttcgatac attcaccaga cttattatca tttcagagga
     9721 attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc aaactgaact
     9781 tctcctttag gatgttttca ctccccgaaa tggggataaa aggcaaataa aataactaaa
     9841 ggatttattc aatgaaaaga aaagtactgg cacttttaat ccctgccctg ctcgccgcag
     9901 gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat ctgtatggca
     9961 aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgac cagagctatg
    10021 cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca acttactggc tacggtcaat
    10081 gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca tggacccgtc
    10141 tggcatttgc cggactgaaa tttgcagatt acggttcttt cgattacgga cgtaattatg
    10201 gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc taaatttggc ggtgactctt
    10261 ataccaatgc agacaacttt atgactggtc gagccaatgg cgtcgcgact tatcgtaata
    10321 ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa ggtaacaacg
    10381 aaggagccag taatggtcag gaaggcacca ataacgaacg cgatgttcgt catgaaaacg
    10441 gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc gctggtgcgg
    10501 catacacctc ttctgaccgc accaatgacc aggttaacca tactgcggcg ggtggtgata
    10561 aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac ctagcaacca
    10621 tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgca gttgcgaaca
    10681 aaacccagaa ctttgaagtt actagtacca gtttgatttt ggtctgcgtc cggcagtctc
    10741 tttcctgatg tctaaaggcc gtgaccttca cgctgctggt ggtgcagaca acccggcagg
    10801 tgttgatgat aaagatctgg ttaaatacgc cgatgttggc gcgacttact atttcaataa
    10861 aaacatgtcc acctatgttg actataaaat caacctgttg gatgaagatg acagtttcta
    10921 cgctgccaac ggcatctcta ccgatgatat tgtcgcttta ggtctggttt atcagttcta
    10981 aatcctcctg cccgctgtta tggcgggctt tttctgctta ttcttcctcc tttgatctaa
    11041 attaaaaatg tgaactccgt cattacacaa aaagtgtcat ctggcgttac actttatgcg
    11101 gatactaaaa caggaggttt tatgaacaga acgattcttg tccctatcga tatttcagat
    11161 tcagaattaa ctcaacgcgt gattagccac gttgaggcag aggcaaagat tgatgatgca
    11221 gaggttcatt tcctgacggt aataccttca ctgccctact atgcctctct gggtttagcg
    11281 tattccgcag aattaccggc aatggatgac ctgaaagcgg aagccaaatc gcaactggaa
    11341 gagatcatta aaaaatttaa actgccaacc gacagagtgc atgtccatgt tgaggaaggc
    11401 tcgcccaaag accgcattct ggaattggcg aagaagatcc ccgctcatat gatcatcatt
    11461 gcttcccatc gaccggatat caccacttat ctgctcggtt ccaacgccgc agctgtagtg
    11521 cgtcacgcag agtgctccgt gctggttgtg cgctgacact aacgcccgca cattgctgcg
    11581 ggctttttga ttcatttcgc aaatgtgctg acattttccc ctctaatccg taccatacac
    11641 gccacagttt ttatatcaga tttcttatgc tgagcgttcc ggcatgacgc atactcttct
    11701 gatgccatat aacgaattga gtcgctttta aatgtcgcaa aatcaagaaa ttagtaagaa
    11761 agaacaatac aacctgaaca aattacaaaa acgtctgcgt cgtaacgtgg gcgaagccat
    11821 tgctgacttc aatatgattg aagaaggcga tcgcatcatg gtttgcctct ccgggggtaa
    11881 agacagctat accatgctgg agattctgcg caatttgcag caaagcgcgc caatcaattt
    11941 ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc ttcccggaac acgttctgcc
    12001 cgagtatctt gaaaagctgg gcgttgagta caagattgtt gaagagaaca cttacggtat
    12061 cgtgaaagag aagattccag agggcaaaac cacttgctca ctgtgttctc ggctacgtcg
    12121 cggtatcctt tatcgcaccg caacggaact ggggacgacg aagatcgcgt tgggtcacca
    12181 tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc tacggcggca agatgaaagg
    12241 tatgcctccg aaactgatga gcgatgatgg caaacatatc gttattcgtc cactggccta
    12301 ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa gcgttcccga ttattccgtg
    12361 caacctgtgc ggttcacagc ctaacctgca acgtcaggtg attgctgaca tgttgcgtga
    12421 ctgggataaa cgttatccag ggcgtatcga gacgatgttc agcgcgatgc agaatgtggt
    12481 gccgtcgcat ctgtgcgata ccaacctgtt cgatttcaaa ggcattaccc acggttctga
    12541 agtggttaac gggggtgatc tggcgtttga tcgcgaagag atcccactac aaccggcggg
    12601 ctggcagcca gaagaagatg aaaatcagtt ggatgagtta cggctgaatg tggttgaagt
    12661 gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg ctactcttat ggaggccgga
    12721 taagacgcgg ccagcgtcgc atccggcaat cccgaataag atgtttactc ttgcacccgg
    12781 caattcaaca tttcattatt ttaataaccg cacccggcac gtttttcctt taatcttccc
    12841 gccctgtaac tgtttccatg ctttatgagc aacagcctga cggaccgcga catagacatg
    12901 cgccggatgc acggcgattt tgccaatatc tgcgccatca agcccgatat ctcctgtcag
    12961 tgcacctaat acatcacccg ggcgcatttt ggcttttttc ccgccatcga tacacaacgt
    13021 tgccatttct gcttccagcg tcacaatgga actattagct ggcggcgttt gccagttaag
    13081 ttttatctgc aacatgtcag aaatgatatt ggcccgctgt gcttcttccg gagcacagaa
    13141 actgatcgcc agaccgctat ttcctgcacg agctgtacga ccgatgcgat gtacatgaac
    13201 ttcagggtcc cacgccagct caaagttcac caccagctca agcgatttaa tatccagacc
    13261 acgcgcagca acatcagtcg caaccagtac acgggcgcta ccgttagcaa aacgtaccag
    13321 ggtctgatcg cgttgctcca gatcgccgtg taacgacaat gcactttgct ctacttcatt
    13381 cagcgcgtcg cagacagcct ggcaatcttt tttggtattg caaaacacca cacaagagga
    13441 tggctgatgc aagcttaata accgttgcaa cagaggaatt ttgcctttac tggatgtctc
    13501 ataaaattgt tgttcaatgg gtggcaaagc atctgttgag tcaatttcaa tcgccaaagg
    13561 atcgcgttgc actcgtccgc tgattgcggc gatggcttcc ggccaggttg ccgaaaacag
    13621 aagcgtctgt cgagatgcag gcgcaaaacg gatgacatca tcaatggcat cgctaaatcc
    13681 catatccagc atgcggtcgg cctcatccat caccaaagta ttcagcgcat ccagtgatac
    13741 cgtgcctttt tgcaagtgat ccagcaaacg acccggcgtt gccacgataa tatgcggcgc
    13801 atgttgcaac gaatcacgct gcataccgaa cggttgacca ccgcacaacg tcaaaatttt
    13861 ggtatttggc agaaaacgcg ccagccgacg caattcacct gccacctgat ccgccagttc
    13921 acgcgtagga cacagcacta aagccagggt ttgaaatagc gacgcatcaa tttgctgtaa
    13981 caagccgagg ccaaaagccg ccgttttgcc gctgccggtt ttcgcctgca cgcgaacatc
    14041 ttttcctgca aggatcgccg gaagcgcggc ggcctgcacc ggcgtcatgg ttaaataacc
    14101 caactcatta aggttcgtga gttgggcggg aggcaaaaca ttcagggtag aaaaagcggt
    14161 cacaatctat tctcgtggtc atcgacgcaa agttagcagg cgcgtatcct cgcagatcta
    14221 cgctcacgat gcgacaattt aatcggttct tcatcgggtg gtgggtcagg catgggttgc
    14281 gggcgaggga tcggatcggg cactggaaca ggatcgccag gaatcggttc agggacagga
    14341 atttgcaaat aaataagtgt cgtcatattt ccctccggtc attgggtgga ctcttaaagg
    14401 gtagacgctg ataaataaca ggcaaaaaaa agccgactca tcaaagtcgg cgtcgtacga
    14461 atcaattgtg ctatgcagta attcaaaaaa ggaagtaaga caatatggag cgcaacgccc
    14521 atcgcttgac gttgcattca cctgcaaggg agatattgcc ctgaatgggt agagagttta
    14581 ttgacttcgc tcaaactttg cggcgttttt gtatacagac agccgaaaaa attgcttttg
    14641 ttacaaccat ttactacggt gcaaccataa agcaacacca ccaataagaa caactaacag
    14701 aatacaaaaa attgaaaatc cgaattgcca cccgccgcca gggatcccac caaggttgac
    14761 gccaaataac ccggtcagaa aggtactggg taaaaagacc attgccatca acgacattgt
    14821 ataggtacga cgagctaaat tttcctgcat cacctgagcg atttcatccg ccatcacgcc
    14881 agtccgtgct atacaggcgt cgatttcgtc aaggccgcgc ccaaggcgat cggcaatatc
    14941 ctgcatccga cggcgttggt catcgctcat ccacggcaaa cgttcactgg caagacgagc
    15001 ataaacatca cgttgcggtg ccatatagcg acgcatcaca attaattgtt tgcgcagcag
    15061 agccaggaat ccacgcggtg gaatttgctg atcaaggaga ttatcttcaa ggtcgataat
    15121 tttatcgtgc agctgctcga taaattcact ggaatgatcg gtcaacgcat cgcacacatc
    15181 caccagccat cccccgcaat cggtcggacc cgtgccctct tccagatcgc tcaccacatc
    15241 gtccagcgcc agcactttgc gttgtcgggt cgaaacaatt aaccgcccgt ccatatatac
    15301 acgcatggcg accagttgat cggggcgttc atcggtgctg ccgtttatac agcgcaatgt
    15361 aatcagcgtg ccttcaccga aacggctgac tcggggccgt gtgctctcgc ccgccagcgc
    15421 atcacgtacg ttattgggaa gcagcggtgt tgtcgccagc cattgggcgc tatcatggtg
    15481 tacataatta aggtggagcc agcagggatg cgcttcatca atcacatctg tattttccag
    15541 cggtttaacg ccgcctctac catccagcat ccaggcaaat actgcatccg ggacattaac
    15601 gtccgatccc ttaatcgctt ccacagtgcc tccatcatca acgcattatt ttgtagtcta
    15661 gccttctggc cctgttacgc aacatctcat caccccatta ccctgaaatg attaataaaa
    15721 ttctgtctaa attgaataca aaaagcaaaa tgcttttccg tatacaaacc gtgtgaagtg
    15781 ttaaatagcg tctatcatta tcagaattat ctgatcatat gacgtggctt ttttgcgatc
    15841 ggatagcaac aaaaattgat aaaaataacg ggatctcaat gattacgcac aacttcaata
    15901 ccctggactt actcaccagt cctgtctgga ttgtttcgcc ctttgaggaa cagttaattt
    15961 atgccaatag cgcggcgcga ctgttgatgc aagacctcac gtttagtcag ctacgaaccg
    16021 gaccctattc cgtctcctca caaaaagaac tgccgaaata cctctccgat ctgcaaaacc
    16081 aacacgatat tatcgaaatc ctcactgttc agcgtaaaga agaggaaaca gcattaagct
    16141 gtcggcttgt tttgcgagag ctgacagaaa cagaaccggt gattattttc gaaggtatcg
    16201 aagcgccggc aacgctgggt ttaaaagcca gtcgctcggc aaattatcag cgcaaaaaac
    16261 aaggttttta tgcgcgcttt tttctgacta actctgcacc aatgttgttg attgacccgt
    16321 cacgagatgg acaaatcgtc gatgctaacc tcgccgcgct caatttctat ggttataacc
    16381 atgaaacgat gtgccagaaa catacctagg aaataaatat gcttgggcgt cgcgtcatgc
    16441 ctatcatgca tgaaatttcg catttacccg gcggtcataa ccctttgaat tttgttcata
    16501 aactggcgga tggttctact cgtcatgtgc agacctatgc cggaccgatt gaaatttatg
    16561 gcgacaagct catgttatgt attgtgcatg atattactga gcaaaaacgg ctggaggagc
    16621 agctggaaca tgctgctcac catgacgcga tgaccggatt actgaatcgg cgacagtttt
    16681 atcacattac ggaaccaggc caaatgcagc atctcgccat cgctcaggat tacagcttgt
    16741 tgctcatcga caccgatcgt tttaaacaca ttaacgatct ctatgggcat tctaaaggtg
    16801 atgaggtgtt atgcgccctc gcccgccccc tcgaaagttg cgctcgcaaa ggcgatttgg
    16861 tgtttcgttg gggaggcgaa gagtttgtct tattgctacc aagaacccca ctggataccg
    16921 cgctttcgct ggctgaaact atccgcgtaa gcgtggcaaa agtgagtatt tcgggcttac
    16981 cacgctttac cgtcagcatt ggtgtggcgc atcacgaagg aaatgaaagc atcgatgaac
    17041 tgtttaaacg cgttgatgat gctttgtatc gggcgaaaaa tgatggacgc aaccgcgtgc
    17101 tggcggcata agccgcggat gcgtctcgag atcaacgact gcgcttagcg tggcgctccc
    17161 agttttcttg cttcgcctgc gccgttttac gtagtgcgac gtaacacgcc ccgctgccgc
    17221 catgatgcgg tagcgcggtg caatatgcct gaacatcatc aaattcggtc agccagcgcg
    17281 ccacatagct gcggacaata ttggcatgtg atttatcttc ccgcccttta ccatgaataa
    17341 tcagcacatt acgcaaacca tccgccaggg cttgttgaat gaaactgaac accattttgc
    17401 ggcactcttc caccggctgg cgcaaaagat tcaggctcgc ctgttgcgga tatttaccac
    17461 tgcgcagctt atccagcacc ccatgctgca acccttcctg ccgaaactcc agcggctgac
    17521 ttagcgggat gatgtcgaga aatccggtag tgaggaaatt atcaagctgc agcgtgtcga
    17581 tacgctgcgg cgcacgttgg ttacgcgttg gatgccagtg gacatctgta gcacgtttca
    17641 gcggctggac atcttccatg gcgtcaagaa acagcgattt gtcgtcaagg ttcatgtaac
    17701 ctcctatgca ccttcacacg tcgtgattca gacgggttaa ttgttcatac ttactgcatc
    17761 gttcccttca cagattgaaa caatactcta cttcatacct gtgagtatct gcgcaggcgg
    17821 ggcattttat actagcctgt tagcagtatc gaacagttta gcccgagttt aattcagcac
    17881 aggctccgat caggatgatt tattcatagc aggaaccaac aaaaatgcga taaataacag
    17941 accactgatt agcagaaagg taccgaaaat gccatagttt gcgcctccct cagcatcaat
    18001 tgactcaata atttggttga agccagtatt tccgatgtct ggtcacttct actgcgatta
    18061 aaaaataaag cacttctgga aatgaagtca ttcaccgtat ggggaacaga aaccattttt
    18121 tcacccattc tcgaaaaatg aaaattagtt cgattattga gaatagagcc acacagggca
    18181 ataccaatcg cgcccccaag attacgtgtt aaattaaata caccagaggc taacttcagc
    18241 cgttcttttg gaattccccc cagcgttaat gttacgatgg gggccatcgc aaactgctgc
    18301 aaaatccctc taattgcctg tggaaacagt aattcctgcc atccccattc gtgggttata
    18361 ggggtaaaaa gatacattga aaacacaaat ccgcccagcc ccgccatcaa cagccatcgc
    18421 agatttattt ttttacttaa ccagaagtaa aagggaacag aaaataactg gaaaatacct
    18481 gttgtgcaaa cggcaaaacc tatctcttcg gcatttaatc cgcgaatctg ccccaaaaac
    18541 acaggtatta aatatacagt agagaatatc ccaacccctc cagaaaaaga aaaaaaacat
    18601 cccagagtga aatttttatc cttaaaagca tgaagatcca ttatcggatt ggatattgta
    18661 agtgtacgcg cagcaaataa aataaaactc accaatgcca gaactgatgt cagcaaaata
    18721 gtattatcat ccagccatcc ccagcgcgct ccttcttcaa gggtatattc cagacatccc
    18781 agagtcatgg ccaacaggat aatactcgga taatcagcaa ctttcagtaa agagagatca
    18841 ggtttatcaa aattaaccag aaaaggaata ctcaaaacta aatatatgcc aggtaatata
    18901 ttgatataaa atagccatcg ccagtcgagg ttatcagtga gccagccccc cagagttggc
    18961 cctagcgtcg gtgacagtga ggccagcgca ctgactaccg cagcagccaa ccccaactct
    19021 ttaccctggt aataaataaa cgccgtcgtg aaaactaaag ggatcatgga tgcgccagca
    19081 gctccctgta aagcacgaaa caaaatcatg atctgaatat tccatgcaag tccacacgcg
    19141 atactcatta aagtaaatat tcccgcggat agagtaaaaa gccaacgagt ggaaaatacc
    19201 cgtgacagcc acccagagag cggaataaca ataatctcag caattaaata gctggtctga
    19261 agccaggcca tttcgtcttt acctgcggac agaccaccac ctatttcatt tagggatgac
    19321 gatacaattt gaatatcaag ctgagcaata aataatccca tacacatact ggcgaaagca
    19381 atatgtttac gcatagacat catctctcat caacactgac ggtgacggac agtcccgggc
    19441 gaagaagttg ctttaattcc tttgcgtcgt caaatgcaat ccgtaccggg acacgctgaa
    19501 caattttggt gaaattgcca gtggcatttt ctatcggaat caagctgaaa ctggctcctg
    19561 tcgctgggga aaggctttca atgtgaccgt gaaacacttc ccctttgaga atatcagcac
    19621 ggatctcagc cttcattcct ggcttcattc ccgctatttg gttttcttta taatttgcat
    19681 caacccacaa ctcactgact ggtactagtg aaactaacga tgtgccgcct tccacccaac
    19741 tgcctgtatg ggcgctgcgg tttgcgacaa tgccatcaat cggcgca
//
LOCUS       DADLTF010000073        19639 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.073, whole genome shotgun sequence.
ACCESSION   DADLTF010000073 DADLTF010000000
VERSION     DADLTF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.073"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1224
                     /locus_tag="JN749_002383"
     CDS             <1..1224
                     /locus_tag="JN749_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708695.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HAY7368768.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="JN749_002384"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="JN749_002384"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HAY7368769.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="JN749_002385"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="JN749_002385"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HAY7368770.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="JN749_002386"
     CDS             2714..3427
                     /locus_tag="JN749_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HAY7368771.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="JN749_002387"
     CDS             complement(3399..4076)
                     /locus_tag="JN749_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7368772.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="JN749_002388"
     CDS             complement(4070..4747)
                     /locus_tag="JN749_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAY7368773.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTCSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="JN749_002389"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="JN749_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
     gene            complement(5443..6021)
                     /locus_tag="JN749_002390"
     CDS             complement(5443..6021)
                     /locus_tag="JN749_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAY7368774.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="JN749_002391"
     CDS             complement(6044..6475)
                     /locus_tag="JN749_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7368775.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELEFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="JN749_002392"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="JN749_002392"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HAY7368776.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHSEAHIAAGAKKVLFSHPGSNDLGATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="JN749_002393"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="JN749_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAY7368777.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="JN749_002394"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="JN749_002394"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAY7368778.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /locus_tag="JN749_002395"
     CDS             10564..11424
                     /locus_tag="JN749_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HAY7368779.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="JN749_002396"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="JN749_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HAY7368780.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="JN749_002397"
     CDS             12291..13031
                     /locus_tag="JN749_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HAY7368781.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..13707
                     /locus_tag="JN749_002398"
     CDS             13198..13707
                     /locus_tag="JN749_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7368782.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPL"
     gene            13831..14093
                     /locus_tag="JN749_002399"
                     /pseudo
     CDS             13831..14093
                     /locus_tag="JN749_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451535.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JN749_002400"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="JN749_002400"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HAY7368783.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JN749_002401"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="JN749_002401"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HAY7368784.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JN749_002402"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="JN749_002402"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HAY7368785.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JN749_002403"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="JN749_002403"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HAY7368786.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
BASE COUNT         4716 a         4910 c         5279 g         4734 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct acaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagctcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaactcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat agcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcggcg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctc ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagatt
//
LOCUS       DADLTF010000074        19389 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.074, whole genome shotgun sequence.
ACCESSION   DADLTF010000074 DADLTF010000000
VERSION     DADLTF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.074"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(43..1872)
                     /gene="glmS"
                     /locus_tag="JN749_002404"
     CDS             complement(43..1872)
                     /gene="glmS"
                     /locus_tag="JN749_002404"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAY7368787.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="JN749_002405"
     CDS             complement(2034..3404)
                     /gene="glmU"
                     /locus_tag="JN749_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAY7368788.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGAGMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="JN749_002406"
     CDS             complement(3756..4175)
                     /gene="atpC"
                     /locus_tag="JN749_002406"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAY7368789.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="JN749_002407"
     CDS             complement(4196..5578)
                     /gene="atpD"
                     /locus_tag="JN749_002407"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAY7368790.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="JN749_002408"
     CDS             complement(5605..6468)
                     /gene="atpG"
                     /locus_tag="JN749_002408"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAY7368791.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="JN749_002409"
     CDS             complement(6519..8060)
                     /gene="atpA"
                     /locus_tag="JN749_002409"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAY7368792.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="JN749_002410"
     CDS             complement(8073..8606)
                     /gene="atpH"
                     /locus_tag="JN749_002410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAY7368793.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="JN749_002411"
     CDS             complement(8621..9091)
                     /gene="atpF"
                     /locus_tag="JN749_002411"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAY7368794.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="JN749_002412"
     CDS             complement(9153..9392)
                     /gene="atpE"
                     /locus_tag="JN749_002412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HAY7368795.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="JN749_002413"
     CDS             complement(9439..10254)
                     /gene="atpB"
                     /locus_tag="JN749_002413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAY7368796.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="JN749_002414"
     CDS             complement(10263..10643)
                     /gene="atpI"
                     /locus_tag="JN749_002414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HAY7368797.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="JN749_002415"
     CDS             complement(11260..11883)
                     /gene="rsmG"
                     /locus_tag="JN749_002415"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAY7368798.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="JN749_002416"
     CDS             complement(11947..13836)
                     /gene="mnmG"
                     /locus_tag="JN749_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAY7368799.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="JN749_002417"
     CDS             complement(14215..14658)
                     /gene="mioC"
                     /locus_tag="JN749_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HAY7368800.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="JN749_002418"
     CDS             complement(14748..15206)
                     /gene="asnC"
                     /locus_tag="JN749_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HAY7368801.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15358..16350
                     /gene="asnA"
                     /locus_tag="JN749_002419"
     CDS             15358..16350
                     /gene="asnA"
                     /locus_tag="JN749_002419"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HAY7368802.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="JN749_002420"
                     /pseudo
     CDS             complement(16355..17806)
                     /gene="viaA"
                     /locus_tag="JN749_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="JN749_002421"
     CDS             complement(17800..19296)
                     /gene="ravA"
                     /locus_tag="JN749_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HAY7368803.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4631 a         5126 c         4864 g         4768 t
ORIGIN      
        1 gcattacacc ggtcggcagc ccttttttat ctatgatagt gattactcaa ccgtaaccga
       61 ttttgccagg ttacgcggct ggtcaacgtc ggtgcctttg atcagcgcga catggtaagc
      121 cagcagctgc agcggaacgg tgtagaagat cggtgcaatc acctcttcca catgcggcat
      181 ctcgatgatg tgcatgttat cgctacttac aaaacccgca tcctgatcgg cgaagacata
      241 caattgaccg ccacgcgcgc gaacttcttc aatgttggat ttcagttttt ccaacagttc
      301 gttgttcggt gcaacgacga taaccggcat atccgcgtca atcagcgcca gcgggccgtg
      361 tttcagctcg cctgcagcgt aggcttcagc gtgaatgtaa gagatctctt tcagcttcaa
      421 tgcgccttcc agcgcgattg ggtactgatc gccacggccc aggaacagcg cgtgatgttt
      481 gtcagagaaa tcttctgcca gagcttcaat gcgtttatcc tgagacagca tctgctcaat
      541 acggctcggc agcgcctgca gaccatgcac aatgtcatgt tcaatggagg catccagacc
      601 tttcaggcga gacagcttcg ccaccagcat caacagcaca gttaactgag tggtgaatgc
      661 tttagtggac gccacgccga tttccgtacc cgcgttggtc atcagcgcca gatcggattc
      721 gcgcaccaga gaagaacccg gaacgttaca gattgccagc gaaccaaggt aacccagctc
      781 tttcgacaga cgcaggcccg ccagggtatc cgcggtttcg ccagactgtg acaaggtgat
      841 catcaggctg ttacgacgca cggcagattt acgatagcgg aattcagagg cgatttcgac
      901 gtcgcacgga atacctgcca gcgattcaaa ccagtagcgg gaaaccatac cggagttata
      961 agaagtacca caggcgagga tctgaatatg ctcaaccttc gacagcagtt cgtcggcgtt
     1021 cggtcccagc tcgcttaaat caacctgacc gtggctgatg cgcccggtaa gggtgttttt
     1081 tatcgcgttc ggctgttcgt agatctcttt ctgcatgtag tgacggtaaa tacctttatc
     1141 gcccgcgtca tattgcagat tggattcgat atcctgacgt tttacttccg cgccagtttt
     1201 atcgaagatg tttaccgaac ggcgagtgat ttccgcaata tcgccctctt caaggaagat
     1261 aaagcgacgg gtcaccggca acagcgccag ctggtcagaa gcgataaagt tttcgcccat
     1321 acccaggcca atcaccagcg gactaccaga acgtgccgcc agcagggtat ccgggtgacg
     1381 ggagtccatg atcactgtac cgtacgcacc gcgcagctgc gggatagcac gcagaacagc
     1441 ctcacgcaga gtcccgcctt gtttcagctc ccagttcacc agatgggcaa tcacttcggt
     1501 gtcggtttca gaaacgaagg tatagccacg cgcttttaac tcttcacgca gcggttcatg
     1561 gttttcgatg atgccattat gcaccaccac aatgtgttca gaaacatgcg gatgcgcgtt
     1621 agcttctgaa ggttcaccgt gggtcgccca gcgagtgtga gcaataccgg tgccgccatg
     1681 cagaggatgt tcttccgctg cctgagccag catctggact ttaccgaggc gacgcaggcg
     1741 agtcatatga ccttccgcat caacaacggc cagaccggca gagtcatatc cgcggtattc
     1801 cagacgacgt aaaccttcaa gaaggatttc tgctacatca cgttgcgcga tcgcgccaac
     1861 aattccacac atagtttttg attccgattt atatcgttgt cggtcaacct gtatgcccgt
     1921 tttttcgggc gccccgagcc ttgtagagag tggggttatt tttatagtta ctgcttgtgg
     1981 gagggagatt atgttatctc ctcatcccag atgaccggat tacccggcca gaattacttt
     2041 ttctttaccg gacgacgcca gccttctttc tgagtctgcg gcacacggct gatagctaat
     2101 gcattttcgc cgacattacg cgtcacagtt gtacccgcag caatggtcgc gcctttgcct
     2161 actgttaccg gggccaccag ctgagtgtcg gaaccgacaa acacatcgtc gccgataatg
     2221 gtcttaaatt tattcgcacc atcgtagttg caggtaatgg ttcccgcgcc gatgttaacg
     2281 ttatcgccaa tttccgcatc gcctaggtaa gtcagatgcc cggctttcgt gcctttaccc
     2341 agacgcgctt ttttcatctc aacgaagtta ccaacgtgtg caccttccag caactcagca
     2401 ccaggacgca gacgggcaaa cgggccaata gtacaggccg ctgccagatt cgcgtcttcc
     2461 acaacggtat acggactgat ttcgcaatca tcgccaatca cgctgttttt aatcacgcaa
     2521 ccggtgccga ttttcacgcg atgaccgaga gtcacgttgc cctcgatgat aacgttagta
     2581 tcaatttcaa catcgcgccc gtgagtaagc gtaccgcgca gatcaaaacg cgccggatcg
     2641 cgcagcataa cgcctgctaa cagcagtttt tcagcctgtt cggactggta aacgcgctcc
     2701 agacgggaga gttgcaggcg gttattcaca ccttctactt cgcttaaacg ttgcggatga
     2761 acggcgacga tttcacgccc ttcctgatac gccagcgcaa taatgtcggt gatgtagtat
     2821 tcgccctgag cattattgtt ggtcagcttc gccagccagc gtttcatacc tgcgccgttg
     2881 gcaatcagaa tgccggtgtt gatctcctga atctgacgct gctcgtcggt ggcatctttg
     2941 tgctcaacaa tgccggtaac tttgccattt tcacgggtga tacgtccata accggtcgga
     3001 tcatccagtt tcaccgtcag cagaccaatg ccaccctggg gtttagcatc acgcaggcgc
     3061 tggagtgttt cgacagagat cagcggcacg tcgccgtaga gcattaaaat gtcttcatca
     3121 tcggcaaaga aaggtgcggc ctgctgcatt gcatgacccg tacccagctg ctctgcctga
     3181 agcacccagt taaggttgtc gtctttcagc gcctgtttaa gcagatcgcc gccgtgaccg
     3241 tacaccaggt gaacgtgcgc tgcgcctaat tcattcgcag catcaatgac atgctgaacc
     3301 atcgctttcc cggcaagggt atgcagcact ttcggaagat cggaatacat gcgcgtgcct
     3361 ttgcctgcgg caaggatcac tacgctcata gcattattca acatacgcgt cctgactgta
     3421 atttgagaac gaatttaaac cgcttcacct tgaaaaaact acattttttt catcgtgaaa
     3481 tggacagagg ataaattgtt caataacgga ttatccccgc tgacgaaagt gccattttcg
     3541 accatcgtca cgccttttgt ctgcagaaaa ttaaggcagt accttcattt aagcgtcagg
     3601 tggatgtttt tgctcttatt tcgatcaatg aataaacggg aaaacagggg ggttttatat
     3661 cagcaggatc tatgtgaacg ctcttcagga cgggtcaacg cgtaaaaaaa agccagcctg
     3721 tttccagact ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg
     3781 ataacgcgca gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg
     3841 tcgccgtgag agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct
     3901 tcgtcgagat cctggccgcg aattgcagtg tcggccagaa cggtcacgtt gccaggctgc
     3961 acttcaagaa tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg
     4021 cgaatcatac caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt
     4081 tcaccttcgc tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct
     4141 gcgctgacga cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa
     4201 gttttttggc tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg
     4261 gcaggtgatc gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg
     4321 agacgtattt acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga
     4381 agcgctggat cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca
     4441 tacccaggat ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc
     4501 cacgcgcggt gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg
     4561 agtccagcgg gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca
     4621 cggttgcgtc aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag
     4681 gtacgtatac cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct
     4741 gcagaacgcc catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc
     4801 ccagcagtgc ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca
     4861 gcagaacgtc acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa
     4921 cgcgcagacg gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga
     4981 taacgttgga gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta
     5041 cgcccgcaaa cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca
     5101 tgtttacggt tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga
     5161 acggacacat caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca
     5221 gctcttcgta ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc
     5281 ctttcatgtc gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta
     5341 ccgggacttc gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg
     5401 aggaacccat tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca
     5461 ccagacgctc attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct
     5521 gagggaattc gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct
     5581 taaatcctct acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag
     5641 ttcctgagta atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag
     5701 gctgccgcca ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc
     5761 caggttttca accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc
     5821 cagcaacgcc ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc
     5881 atcatctgat gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt
     5941 aataaatttg ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa
     6001 catcactttt accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac
     6061 ctgggcaaca acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat
     6121 tgcgaggtcg cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt
     6181 gaacaggtta atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc
     6241 cacgcgttta acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc
     6301 aaggtgacca atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc
     6361 ctgcgattta cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt
     6421 gttctggacg cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc
     6481 aatgccttgc ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg
     6541 aaggaatcga ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg
     6601 ttgatctctt gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg
     6661 aagctgccaa ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga
     6721 accagagact gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc
     6781 actttctgac cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga
     6841 gagaacgctg ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt
     6901 ttcatgatct tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc
     6961 gcaggacgaa taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg
     7021 gagattacgt tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc
     7081 agtgcggtca gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat
     7141 tcggcgttaa cacgtgcagc acgctccagc agacgagagt ggaggtagaa aacgtcgccc
     7201 gggaatgctt cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca
     7261 gcctgtttag acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat
     7321 tcgcccattg cgcaaccggc atacggtgcc aggtattgca gtgcagcgga ttcagacgcg
     7381 gttgctacca caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg
     7441 ttagaaatgg tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg
     7501 cgctggttga tgatggcatc gatagccagt gcggttttac ctgtctgacg gtcaccgatg
     7561 atcaattcac gctgaccacg accgattggg atcatggagt caacggcttt ataaccggtc
     7621 tgtaccggct gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca
     7681 gagaagccgt cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc
     7741 acacggccca gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact
     7801 ttcatgcctt cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc
     7861 tcgaggttca gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata
     7921 caatcggcca ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg
     7981 ttgtgagctt cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt
     8041 tcggtggaat tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc
     8101 tcaagacgac cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct
     8161 gccattacag acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc
     8221 atcgcagcag aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct
     8281 acctcagcgg tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg
     8341 agcgcgttaa gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc
     8401 agttgctcac cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca
     8461 gagagaagct ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc
     8521 tgccagcgtt ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg
     8581 cgagctaccg taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt
     8641 tatccacgat gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc
     8701 cagcaacagc caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg
     8761 cttcaatttc agcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt
     8821 tcgcttcgtc cagaatctgc gagcggagtt tattcgcctg ctcgatgatt acctgggctt
     8881 ccgctttcgc ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat
     8941 gtgctcgttc tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg
     9001 ccattaatgg cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg
     9061 cctggccgag gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc
     9121 tgatattgct ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga
     9181 cccagaccta cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga
     9241 gtacgcagca gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg
     9301 aggatgccga taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc
     9361 atgtacagca gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa
     9421 cgtagtagtg ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag
     9481 aaccatgaag atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac
     9541 attcaggatc cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc
     9601 accggcatac atgttaccga acagtcgcaa accgagtgaa actggtttgg acagcaggct
     9661 taccccttca aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt
     9721 caactctttc gtgaagccgc cgatgccttt cattttgatg ttgtagaaca gaatcaggat
     9781 aaatacgccc agtgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag
     9841 tgcaggcaga cccagtacat gttcagcaat gtacggcagc aagtcgatag gcagtaaatc
     9901 catcagattc atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt
     9961 gcttttgcca tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat
    10021 cgcggtctga aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa
    10081 aaccaagaac aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca
    10141 gaaggtggct ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag
    10201 gttattcagg tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc
    10261 ttttaccctt tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc
    10321 cacgtaacga tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac
    10381 accaacatcg ccagaacttt gaaagcttcg ccaaatgcga atgtccaagc cacccggcct
    10441 ttcgctggtg tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga
    10501 aagactgcca ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac
    10561 agcaatccac ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt
    10621 cgactcacga gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc
    10681 gtgtcgtata aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata
    10741 cggtgcgccc ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat
    10801 ttttccagtg catacaattg cgacttttcc gctacccctg ttcgatcatg aaaaactgta
    10861 aataacgcgt aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg
    10921 cacagcatat ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta
    10981 aaaacagata ctgttttaat aaatgacatt tacacaacaa aaaccaccca ttgacatttt
    11041 taataatgtt ttaacagcca atgatggttc ttagcgccga tttttagcag actgatattt
    11101 tcactaatga cttattttct gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag
    11161 agtgaacgtg gcgttaaatg taaccagtta tatcagtaga aaacctggtt gttgttaaca
    11221 gtctaaccgg tcaatttttt atgatttttt tgataaaaat taaattttat ttgctttaat
    11281 caccaccaga tgacgttcgc catccagggc tggaacctga agtttaacca ctgattcgac
    11341 ctgatattct tcgggcaaca aagcgatttc atcttccggc atttgccctt tcagcgcgta
    11401 gaaacggcct tgctcaccag gaagatggtg gcaccagctc accatatcgt tcagagaggc
    11461 aaaagcgcgg ctaattacgc catcaaatgg cggctctgaa ggaaactctt ctaccctgct
    11521 ctgtactggt tcaatattct ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac
    11581 gcgtttacca aggctatcca acagagtgaa atgggcttca ggacgcacga tagagagtgg
    11641 aatgcctggc agtcctggtc cggtgccgac atcgataaac cgttcacctt gcagatacgg
    11701 tgccaccaca atgctatcga gaatatggcg taccagcatc tcattaggat cgcggaccga
    11761 agtcaggttg tacgctttgt tccatttatg cagcatattc acgtaggcaa taagctggtt
    11821 tttctggtga tcagtaagcg aaatacctgc gtctttcagc agtaaggaga gtttgttgag
    11881 cacggtgatt acctgttctt gatgcgttgc ttggtaagcg ggtgcttacc aggcattttt
    11941 aatgcgttat gcgctacgac gcagcatacc ctgttttttc agccacacca gcagaatgga
    12001 gatggccgca ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg ccggtttgtg
    12061 atcgttaagt ttggcgatca cttcgttaga aagaccggat acctggcggt aatccagtgt
    12121 cgcgggtagc agggtgttct cgttacgcag ctgcttttcg atctcatctt gctggcgcgc
    12181 gatataacct tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa
    12241 cgcaggggca aacggcgtca gcgtggttaa tttttcataa gtcatttccg gacgacgcag
    12301 cagatcttca ccactggctt cacgggaaag cggcgcagtc aggtgagcat tcacttcggc
    12361 tgcagcttcc gccgacgggg ttacccaggt cgatttcaga cgctgacgct cacgctcgat
    12421 attctcaagt ttctcgttaa agcgcgccca acgttcgtca tccaccaggc ccagttcacg
    12481 accgatttca gtcaaacgca gatccgcatt atcttcgcgc agcatcagac gatattctgc
    12541 gcgcgaagta aacatacgat acggttcttt ggttcctaaa gtgcacaggt catcaaccag
    12601 tacgccgaga tacgcctgag aacgtgccgg agcccaacct tctttgtcag cagacagacg
    12661 ggcagcgtta agaccggcca gcaaaccttg cgcagcggct tcttcgtaac cggtagtgcc
    12721 gttaatctga ccagcaaaga acagcccctg gataaacttg ctctccagcg tcggtttcag
    12781 gtcgcgagga tcgaagaagt catactcaat ggcataaccc ggacgcacga tcttcgcgtt
    12841 ttccatcccc tgcatagagc ggacgatttg catctgcaca tcgaacggca ggctggtgga
    12901 gataccgttc ggataaattt cattagaggt cagtccttcc ggttcaagga agatctgatg
    12961 ctgatttctg tctgcgaagc gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc
    13021 gacaccttcg atcacccctg catacattgg gctacgatcg aggttactgc ggatcacatc
    13081 atgggttttc tcgttggtat gagtgatata acacggcacc tgctggggat gcagggacgc
    13141 attgcccata aacgagaata ccggcattgg gttatcgcca tgttgttgcg ccagcacgct
    13201 aaagtcgatg gttcgagcat caatacgcgg cggagtaccg gttttcagac gaccaacgcg
    13261 cagcggcagt tcacgcaaac ggcgagagag cggaatggat ggcggatcgc cagcacggcc
    13321 accgctgtaa ttatccagac cgatatgaat tttgccgtcg aggaacgtcc caacggtgag
    13381 caccacagct ttggcacgga acttcagtcc catttgggta acagcaccga ccacgcgatc
    13441 gttttcgaca ataagatctt caaccgcctg ctggaagatc atcaggttcg gttggttctc
    13501 cagcgccgta cgtaccgcct gacggtagcg cacacgatcc gcctgagctc gggtagcgcg
    13561 aaccgccggt cctttgcttg cgtttagtat cctaaactgg atacccgctt gatcgatcgc
    13621 tttcgccatc agaccgccga gtgcatccac ttcttttacc agatgtccct tcccaatacc
    13681 gccgatcgcc gggttgcagc tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag
    13741 agtctgttga cccatacgcg ccgcagccat cgcggcctcg gtgcctgcat gacccccgcc
    13801 aatgatgatg acgtcaaaag gatccggata aaacatggtg attgcctcgc ataacgcggt
    13861 atgaaaatgg attgaagccc gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga
    13921 cctgggatcc tgggtattaa aaagaagatc tatttattta gagatctgtt ctattgtgat
    13981 ctcttattag gatcgcactg ccctgtggat aacaaggatc cgacttttaa gatcaacaac
    14041 ctggaaagga tcattaactg tgaatgatcg gtgatcctgg accgtataag ctgggatcag
    14101 aatgaggggt tatacacaac tcaaaaactg aacaacggtt gttctttgga taactaccgg
    14161 ttgatccaag cttcctgaca gagttatcca cagtagatcg cacgatctgt atacttattt
    14221 gagtaaatta atccacgatc ccagccattc ttctgccgga tcttccggaa tgtcgtgatc
    14281 aagaatgttg atcttcagtg tttcgcctgt ctgttttgca ccggaatttt tgagttctgc
    14341 ctcgagttta tcgatagccc cacaaaaggt gtcatattca cgactgccaa taccgattgc
    14401 gccaaagcgg accgcagaaa gatcgggctt ctgttcctgc aatgcttcat agaaaggaga
    14461 aaggttgtcc ggaatatctc cggcaccgtg ggtggagctg ataaccagcc agatccccga
    14521 ggcagataaa tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc
    14581 cagcttttca gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc tgccgctgat
    14641 aagagtgata tctgccataa accgccacct ttattaagag tggcgtattg tacgctgtga
    14701 acgcgttggg atctacctgt ggaaaagtat gggattaaaa aagccgatca gggcttgatg
    14761 gtacgcatga tcgggttttg caggacgatc aatgtctcgg tggactgaat ttcatcaatt
    14821 gtttggatct tgttgataag tacatgctgg agagcgtcga tcgaacggca cataactttt
    14881 ataaagatgc tgtagtggcc ggttgtgtaa taggcttcag tgacttcatc aaggctttcc
    14941 agctttgcca gcgcggaagg gtagtctttg gcgctcttta atataatgcc gataaagcag
    15001 cctacgtcat aaccgagctg cttcgggctg acatcaatac gcgccccggt aatgatcccc
    15061 gcctgcttca ttttctctac tcgaacgtga atcgtccccg gactgacgcc aaattgtttc
    15121 gccagttcgg cgtaagcggt gcgcgcattg cccattaatg cttccaggat gccacggtcc
    15181 agattgtcga tcagataatt ttccatagga ttttcttatg cagattgatg attcattcta
    15241 ttttagcctt cttttttaat gaatcaaaag tgtgttaggc tttttattga atgattattg
    15301 catgtgtgtc ggtttttgtt gcttaatcat aagcaacagg acgcaggagt ataaaaaatg
    15361 aaaaccgctt acattgccaa acaacgccaa attagcttcg tgaaatctca cttttctcgc
    15421 caactggaag aacgtctggg gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg
    15481 gatggcacgc aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct
    15541 ctgcctgatg cccagttcga agtggttcat tcactggcga agtggaaacg tcagacctta
    15601 gggcaacacg acttcagcgc gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc
    15661 gatgaagacc gtctttctcc gttgcactcg gtctatgttg accagtggga ctgggaacgc
    15721 gtaatgggcg acggtgagcg tcaattctcg actctgaaaa gcacggtaga ggcgatctgg
    15781 gcgggaatta aagcaaccga agctgcggtt aacgaagagt ttggcctggc accgttcctg
    15841 ccggatcaga ttcacttcgt acacagccag gagttactgt ctcgttatcc ggatctcgat
    15901 gccaaagggc gtgagcgggc gattgcgaaa gatcttggcg cggtattcct cgtcgggatt
    15961 ggcggcaagt tgagtgatgg tcatcgtcac gacgtgcgcg caccggatta tgatgactgg
    16021 agcaccccgt cagagctggg ccatgcgggc ctgaacggcg atattctggt gtggaacccg
    16081 gtactggaag atgcgtttga gctttcttcc atggggatcc gcgtggatgc cgacacgctg
    16141 aagcatcagc tggcgctgac cggtgatgaa gatcgtctgc agctggaatg gcatcaggcg
    16201 ctgctgcgcg gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc tcgtttgact
    16261 atgctgctgc tgcaactgcc gcatatcggc caggttcagt gtggagtatg gccagttgct
    16321 gttcgcgaga gcgtcccttc tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc
    16381 ggcttcgcat cccggtatca aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt
    16441 tgccgtgtgc cgacatcgcc acggcatgaa agcgatgctg atgtacccgc tgcagctctt
    16501 tcactttact cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg
    16561 catcggcatc aaaccattcc ctgctttgca agcgttccat aatggcgcga aaacaactgg
    16621 caagatcggt gccgccacga aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt
    16681 gtgggcctga aagctcataa cggacgatct cggtggaaaa tagcataata taacagcgcc
    16741 ggttttcagc gagagcaatg cgcatcaaag ccaggcagaa cgctttcgca cactgttcat
    16801 taaagccgcc cattgagccg gaagtatcca cacagacaat aaacggcccg cgcggctgtt
    16861 catcgtaatc tttatgtacc accggacgtt cgagcacttt ttcacgccac gactcaccgt
    16921 gcaggcgata ggtgagcaac tgtttttcca ccagccgacg gtaaaactca tactccagtt
    16981 ccgttatccc tagtgtcgcc agttctggcg gcaggagacg taaaatatca tcgctttgtt
    17041 gcagaccatc aacctgctca ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt
    17101 ccatctacgc atcgttgcgc ggtattgatt tggcttcccg agaacgcccc ggctgctctg
    17161 ccaggcgttt cagttccggc tgttcgttaa gaaattcacc gtatttcaca ataaactgat
    17221 agtcgccacg tttaagctga ccggcgctca tatcccacag acgaccagct gcggtattgt
    17281 tatctgcgag aatcggttca agttgtccgc tcagcgtcat gcgttcctga acttcactta
    17341 acagttgttc gcgttcttct tctaatagct gttgatttaa cgtcgttgct tgcacgatca
    17401 gacttaaacg ccaacgctgg agaaaaagcg tgtgtaacgc tgaagttatt gtgctgttag
    17461 catcaaccaa ctgccgggct tgttctgccc atggggaatt cagacgatgc agtaagtcta
    17521 ggatctgtgg tagctgcaca ataaactgcg gcgtggagag gagctggctt tgctgatagc
    17581 acatcacctc ttcggtgagt tccggcggga ctcgggcatc tttcagccga ctgcgcagcg
    17641 cctcacgcca gcggggaaca tcatcagtga ttgccgcctt cagtcgtggg aatttttcaa
    17701 agaagaccgc cagctgcggt gaggccagca gtgcgatgat catctcttcg atcaatccct
    17761 cttcgctgac ggccagcatc acattaagcg tatccagcgt tagcattgtt gtgcctggcg
    17821 aatctgtgca ccgacatcct gtaggctggc ttcaatgcga cctaaccagt cgccagggat
    17881 aaacagacat ttttgctgtt cgctgaataa agcgtgttgc ttacgccagt cgctttcaag
    17941 ctcttccagt tgttgtttta tttcacctgg cagaccttca gccgatgaac cgggaagtgc
    18001 cagcgtactg ccttgtaaac ttacatcgcg tacaacaaga tgttgggtgc tatcaacttc
    18061 cagattcagt ttctgggcaa agccgatacc gttcagtttg ccgcgaattt caccaccttt
    18121 actcagccac tgttccagcg cgctacgctc aaaggagata tgaaccactt ccatatcatg
    18181 cagttttaac ggtttttgca gcagcagagt cagagtggaa gcagtaacgt taacaggaag
    18241 ttgatactgc tgacgacggc tgaaaatgcc gcccagacga attaacgtta aggctgtttt
    18301 atcgctttgt tgctgctgga gttgcaggtg acgttgcaca atcgcgccca ggcgggtcaa
    18361 catcccttgc tgttgccagg catgaccggt catcaataca tcaatttgtt gttgtatcaa
    18421 attcaggctt tgcgcgtcat accacaggca atctttcagc aaaatgagat caaccggggc
    18481 aacagcactg cgaccgctaa aaaaggcgct ggcctgcaat aagcggatcg cttttttcca
    18541 gcgacgatcc gagacataag gcgcatccgg taatttatcc agttgctggc gcagcataaa
    18601 aatcagctca aatacatgat cgggcagcgt aatttcacca atctctttct gccagcgttc
    18661 atattcttca tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg
    18721 actggtcagc atggagcgga aattcgcttt atcttgcact ttatctaacc atagacgaat
    18781 cagcatgcgg tcatataacg cttccagact gctgtctgct tccggcagct cgttggaggc
    18841 cgccaccagt agacgcatcg ggattttttc tacgtgtgca ccgttgcgga actggcgctc
    18901 gttaatggcg gtgagcaagg tattaagaat tgccgggccc gctttccaga tctcatccag
    18961 aaagacgatt tctgcttccg gcaggtaacc gctggttaaa cgttcatagc gcccttcatc
    19021 ttttagcgcc tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat
    19081 cagatattca aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg cgatcaaact
    19141 tttggcaata cctggcgggc caaggaggaa cacactttca ccacttaatg ccgctaataa
    19201 acacaagcgg atggcgtggc tacgttcata aagccccttt tccaacgaac tgctcaggcg
    19261 ggaaattctt tccgctaata aatgagggtg agccataatg aagtggcgtc ctttcgtcaa
    19321 aagttctgcg taaattgcga gtatagacgt ttcttgctgg tggctaaaat agtctcaaag
    19381 gggggtaat
//
LOCUS       DADLTF010000075        18732 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.075, whole genome shotgun sequence.
ACCESSION   DADLTF010000075 DADLTF010000000
VERSION     DADLTF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.075"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN749_002422"
     CDS             complement(<1..1168)
                     /gene="emrB"
                     /locus_tag="JN749_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HAY7368804.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSG"
     gene            complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN749_002423"
                     /pseudo
     CDS             complement(1185..2357)
                     /gene="emrA"
                     /locus_tag="JN749_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN749_002424"
     CDS             complement(2484..3014)
                     /gene="emrR"
                     /locus_tag="JN749_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HAY7368805.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            2985..3086
                     /locus_tag="JN749_002425"
     CDS             2985..3086
                     /locus_tag="JN749_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368806.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN749_002426"
     CDS             complement(3105..3440)
                     /gene="ygaH"
                     /locus_tag="JN749_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HAY7368807.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(3430..4167)
                     /locus_tag="JN749_002427"
     CDS             complement(3430..4167)
                     /locus_tag="JN749_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAY7368808.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(4291..5470)
                     /locus_tag="JN749_002428"
                     /pseudo
     CDS             complement(4291..5470)
                     /locus_tag="JN749_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(5662..6654)
                     /gene="proX"
                     /locus_tag="JN749_002429"
     CDS             complement(5662..6654)
                     /gene="proX"
                     /locus_tag="JN749_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HAY7368809.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(6712..7776)
                     /gene="proW"
                     /locus_tag="JN749_002430"
     CDS             complement(6712..7776)
                     /gene="proW"
                     /locus_tag="JN749_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HAY7368810.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(7769..8971)
                     /locus_tag="JN749_002431"
     CDS             complement(7769..8971)
                     /locus_tag="JN749_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HAY7368811.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(9326..10285)
                     /gene="nrdF"
                     /locus_tag="JN749_002432"
     CDS             complement(9326..10285)
                     /gene="nrdF"
                     /locus_tag="JN749_002432"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAY7368812.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(10295..12439)
                     /gene="nrdE"
                     /locus_tag="JN749_002433"
     CDS             complement(10295..12439)
                     /gene="nrdE"
                     /locus_tag="JN749_002433"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAY7368813.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(12412..12822)
                     /gene="nrdI"
                     /locus_tag="JN749_002434"
     CDS             complement(12412..12822)
                     /gene="nrdI"
                     /locus_tag="JN749_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAY7368814.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(12819..13064)
                     /gene="nrdH"
                     /locus_tag="JN749_002435"
     CDS             complement(12819..13064)
                     /gene="nrdH"
                     /locus_tag="JN749_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAY7368815.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(13312..13641)
                     /locus_tag="JN749_002436"
     CDS             complement(13312..13641)
                     /locus_tag="JN749_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7368816.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     QLSMRKS"
     gene            13793..14143
                     /locus_tag="JN749_002437"
     CDS             13793..14143
                     /locus_tag="JN749_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HAY7368817.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
     gene            complement(14180..14617)
                     /gene="alaE"
                     /locus_tag="JN749_002438"
     CDS             complement(14180..14617)
                     /gene="alaE"
                     /locus_tag="JN749_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HAY7368818.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            15285..15689
                     /gene="stpA"
                     /locus_tag="JN749_002439"
     CDS             15285..15689
                     /gene="stpA"
                     /locus_tag="JN749_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HAY7368819.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(15736..16260)
                     /gene="ygaP"
                     /locus_tag="JN749_002440"
     CDS             complement(15736..16260)
                     /gene="ygaP"
                     /locus_tag="JN749_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HAY7368820.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(16270..16569)
                     /locus_tag="JN749_002441"
     CDS             complement(16270..16569)
                     /locus_tag="JN749_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7368821.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            16752..16910
                     /locus_tag="JN749_002442"
     CDS             16752..16910
                     /locus_tag="JN749_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HAY7368822.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            16994..17443
                     /gene="kbp"
                     /locus_tag="JN749_002443"
     CDS             16994..17443
                     /gene="kbp"
                     /locus_tag="JN749_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HAY7368823.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(17444..18102)
                     /gene="csiR"
                     /locus_tag="JN749_002444"
                     /pseudo
     CDS             complement(17444..18102)
                     /gene="csiR"
                     /locus_tag="JN749_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(18123..>18732)
                     /locus_tag="JN749_002445"
     CDS             complement(18123..>18732)
                     /locus_tag="JN749_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7368824.1"
                     /translation="PGLKAVGSYRSVLELLNIPHAKLIMDCVILLSVTSCLNSALYTA
                     SRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDS
                     SGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVVMLFR
                     PAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
BASE COUNT         4790 a         4751 c         4722 g         4469 t
ORIGIN      
        1 aaccagacag cgtaatggtg gtcagcggca taaagaagca ggccaccgca aacccctgga
       61 taaactgcgg ccaggccgac gcgccaaaat ccatacctgg ttcaaaggta taggcacgcc
      121 agtagaagca gacggcatac ataataaagc tgaaggttac cagccgccgc atatccagtt
      181 tatgcgcgaa gcggccgata atcggcgaca gaatcaccgg aataatccct accggcgcag
      241 aggccaaacc tgcccaggtc gccgtgtaac cgtagacctc ctgcaacaac tgcggcagca
      301 gaacaatagc gccgaagtag agcatatacg cgaggctgat acacaagcag ccgatggtga
      361 agttgcgcga cttaaacaac gacagatcga ctatcgggtt atcgtcggtc agctcccaga
      421 caatcaggaa gcagatagcc accaccgcca ccacggtaag aatgataatt tcctgtgatg
      481 aaaaacagtc cagctcttta ccgcggtcga gcataatctg caggctgccg ataccaataa
      541 ccagcagtgc cagccccacg gcatcaatcc gccgccgttc ggtgcgggtt tcacgtccgc
      601 gcagagtttg cagtgtcatc aacaccaccg ccacgccaat cggcacgttg atgaagaata
      661 tccagcccca gtggtaatta tcgctgatat aaccgccgag gatcgggccg caaattggcg
      721 cgacaatcac cgtcatcgac cacaacgcca gcgcgatcga gcgtttggct ggcgggtagt
      781 tattcagcaa tagactttgc gaaagcggga tcaacggccc ggcgacaatc ccctgaatca
      841 cgcggaagaa gatcagcata ttcaggctgc tggagacacc acacgcccac gacgcaatag
      901 caaaggcgat ggtggaccaa aggaacagtt tcacttcccc gacgcgcttt gccagccagc
      961 cggtaagcgg gatcgagatg gcattcgcca ccccgaaaga agtgattacc cacgttcctt
     1021 ggctgagcga tgagcccaga ttcccggcga tagtggggat cgccacgtta gcaatggtgg
     1081 agtccagcac ctgcatgaat gtcgccagtg acagcgcaat cgtcataatg accagttgcg
     1141 cgccttccag cggtttttgc tgttgcatca cacgcacctc tggattagcc agcgttagct
     1201 tttacgatat cgtcgatcag tttattgaca ggtgctaggc tgatttcacg cgcggtactt
     1261 accgcgaccg gtgttgaacg tactttattt gccagtacct gaccgtcacg gttagtggta
     1321 ttgacgctca ccagcgtgga caaaccaatg cgcagcgggt attgctccag ctgtttctgg
     1381 tccagttcga tacgcacagg tagacgctga acgactttga tccagttacc ggtcgcattt
     1441 tgcgccggaa gcagtgagaa cgcgctacct gtgcccatat ccagaccaac cactttaccg
     1501 gtgtatttca catcatcgcc gtaaatatcg gtggtgatgg tgaccggctg accgatgcgc
     1561 atattggcaa tctgcgtctc tttaaagttg gcatccaccc acatattggt ggctggaacg
     1621 accgccatca gcggcgtcgt tgggctaatt tgcgcacccg gttgcacggc acgacgggaa
     1681 acataaccgg tcatcggact gacaatacgg gtacgctcca gcgccagcta ggcgttacgt
     1741 acttcggtgg cagcctgttg cacggcaggc tgatcttcca gtttagtccc cagaatcatc
     1801 gcctgattgg cattgtattg ttgaatagcg acgtccagtt gcgcctgggc actggtgacg
     1861 gcgtcgcggg cgtgttgcag ctcttcgcga ccaatcaggt tggcattgcc cagcggcaca
     1921 cggcggttgt agtcgctttg tgctttcgcg agggcgattt tttgcacctc aatattcgcc
     1981 tgcaactgtt tgctgttaat catcagctgg tgggtttggc gaacgctgga agccagtgca
     2041 gttttggctt tttcaaacgc ctggcgagca tctgtcgggt cgagagtgac cagaacatcg
     2101 ccttctttta caaaatcggt gttatcggcc cagactttcg tcacgctgcc agacacctga
     2161 gacataattt gaatctgatt ccctgccacg tatgcgtcat cggtttcttc gaaatgacgc
     2221 agtaccaaaa accaataaat ccctatcgct acggcaataa ttataaagag caaggtgaga
     2281 aggaggagca gacgcttacg tttgccgctc ttctttaccg gttgctgcgg ggtttgagtc
     2341 tccgcatttg cgctcatatt gttctccacg atcttcttaa ctcatcggct gagccgacct
     2401 gcttgccaaa aaggccagca tgttgcgatg ctggccggtc attttttctt ttataaatct
     2461 ggatttttga gcgagatgac gcgttagctc atcgcttcga gaaccacacc gtcttgttcc
     2521 atctggtcga gacgggagag caatttgcgg gtgatttgct cgagctgatc tttttctgtt
     2581 gtgctgagcg cggaccagag ttgatgcagg cagttatgct gcggtggtaa aacctcgcgc
     2641 aaaaactcgt gacctttttc cgttaattgc agatgcaggc agcggcgatc gttatcgctt
     2701 tcacgacgtt cgatccaacc gcgtttttcc agttcatcgg caatacgcgt cgcgttggta
     2761 cgggatgatc caagagcaca acttaattca gaaggctgaa tactgtggtt ttcctgagac
     2821 tccagcgtaa tcaacgccat aaacaacgtc tcgttaatcc cctgagcctt cagcatttta
     2881 ttgcggttct ccagcagctt gctttgcatg tgcatgcaaa gacgagtcag aaggatctcc
     2941 tgataaggaa aatcttcgtg gcggctggcg cgaaatttta gcatttgttc aatgggcgta
     3001 aacgaactat ccatttgggt atgacctcat taatttcagc cgatatagta acgacagtga
     3061 caaataaagt aaatgtattg ttttagaaaa atgattcttg tgggttatat aatcgccatc
     3121 actttccagg cgagcccata ggccagcgca ctaagcagtg ttgggatgat aatgctgcgt
     3181 gttttataga aactggcacc cagtaccgcg aagccgacca gcgtgggcac gaaacggcgt
     3241 gtatcgtgca tcacttctgg tgcggtagag acaaccagca gagcgcatat cgaggcgatg
     3301 ccaatggtgt cgagcaaaat acctaccgcg ccacgtttgg ttgggcgggc attacccaca
     3361 cgcaggcgca gcggcaaata gcggaagcaa taattcgccg cgccaaccag taacccaagc
     3421 agcagaacct catagctcat cgggcgctcc ttgccagaat gcctggatta acgcagtgag
     3481 gcagccacag acaatgcctg ccagaatggc gacgggaata gaaaatagcg ttacgcctgc
     3541 aagggcacca actaaagctg cggtaacgca aagagattgt ttgcgctgga aagaggcgag
     3601 caggaaactc ataaagagtg ccggaagcat aaaacctaat gcagcttcaa cggcgggata
     3661 accttgcagc aagccgctgc cggagaatgc ccctattacc gtaccaaata cccacgatga
     3721 ccatgaactg aaggcaatgc cgatcatcca gttctcgctc cagcggcgat tattgcgtac
     3781 cagttttgcg gtggcggcgg caaaaacctc atccgtcagg ccaaacgccc atagggcagt
     3841 tttcgatttt tgcagacgct gaataatacg gctacgcagt gacgggccat acaaaacatg
     3901 gcgaacatcc attgccatga cggtcagtgc agcaacccac aaactactcc cggctgccag
     3961 catcgcggta atgacgaact ggctcgcgcc tgcataaatg atgcaggaga aaaaaacgct
     4021 ttcgagagga gagaatccca gacgggtcgc attcagaccg aacgcaaagg ccaccggaat
     4081 ataactaata acaatcggta aactgtcttt gcatccttcc atgaaggtcg ccgaaccagg
     4141 agcaggctgt ggagtagggc tttccataga gtgtacgctt aacgattgtt aatattataa
     4201 atagactgaa tgaatatctt aaccttatca gactgatggg cttcttaaca cccttataag
     4261 tgtaaagcca cgaaaacggt tgctgattgc ttaatttgcg gcttcctgac gatgaaaacc
     4321 tcgccaccag accagtaagt taaccagggc aatcgtcgcg ccagccagac aaacgccagc
     4381 ccaaccgcca tgttgccagg ctgaggctga aattagcgaa ccggcggcac cgccaataaa
     4441 gtagctggtc atgtaacctg cggtcaggcg attacgcgca tcaggatgta ttcgataaat
     4501 taccgtctgg ttagtgatat gcacgccctg tacggtgagg tccagcacca gaatgccgat
     4561 aatcaacgcc agtacggaag tgtgaccaaa ccagattgcc agccatgaaa gtaacagcag
     4621 aagcagaccg aaagttgtgg tgcgatttgc ccttgtcggc aaaaccgccc gccggacgag
     4681 cgcccaacgc tccggcagct cccgcaagcc caaacagacc aatgtcacca tcgctgtagt
     4741 taaaaggtgg agcggcaagc aaaaaggcca ttgaggtcca gagaatgctg aagttggcaa
     4801 aggtcatgca gcccagcaac gcgcgggtgc gcaggatctt atcgctgatg aacatgctga
     4861 aaacggaacc caacaactgt gggtagttga ggtgggtttc tgatttcatt tgtggcagac
     4921 cacgccataa tgccagcgcc atcagtgcca ttaacaccga agcaacccaa aagacggtgc
     4981 gccagccgcc gaggtttgcc agtaacccgg caacggttcg tgccagcaat atccccaaca
     5041 gcagaccgct cataatggtg ccaaccactt tgccgcgttt atccggtgaa gccagcgtcg
     5101 ctgccagcgg aaccagaatt tgtgccacga ctgagaataa accggttaat gccgtaccga
     5161 ggatcatcat cgccagtgac tgactgctgg cagtgattaa cataccgccc gctgccagta
     5221 aggtcatcga gacaatcagg cggcggcgtt caaacatatc accgagggga acaagaaaca
     5281 gtagacctgc ggcatagccc aactgcgcgg cggtaacaat aaagcctgcc gaactggcgg
     5341 aaagggaaaa gttacgcgcg atggtgtcga gcaatggctg ggcgtaatag ttactggcta
     5401 ccgccagacc ggtggcgata gacatcagca cgatcagcgc cgggctaagc tcatgattag
     5461 gtttagtcat tatttttgtt catcaggtga tgattaagct ggaaatcaat aatagcggat
     5521 gttgatgaat atgggatgaa agatagcttg ttgaaatgtg cgatgtggtt tgtaggcatg
     5581 ataggacgcg tcagcgtcgc atcaggcatt gtgtacagag cggcggatga ggcgaaaacg
     5641 ccttatccgc ccgaataaaa attacttctg cgctgccagc gcctcattca cccagccatc
     5701 gaactgctgc tggtgggctt tgatccagcc atcaacgtgg ccctgaatat cgccttctga
     5761 ggctttgccg tcatgcataa tggcgttctg ggcgttaata tcggccactg gcaactgcat
     5821 aatggcaaac agtttggctg ccgccggatt tttctcggtc caggctttgt tggcaacgat
     5881 atgcatggtg ctgaccggga agccgtaatt cgcgccattc ggcagtttgg tatcggcatt
     5941 tttatcgccc ggcagtgcgg agaacggcac ctgcaaccag accacatctt tccctggctt
     6001 cagctcatta ctcacccagt acggcgtcca ggtgtaataa aacaccggtt tgccctcttt
     6061 gtagcgactg atggtgtcgg ccatcatcgc tgcgtagttc ccctgattat gcgtcacggt
     6121 gttggtcagt tcatacgcgg caagctggtg gttgatcgca ccttcgcagc cccagccagg
     6181 gttacaaccg gttaaatccg ctttaccgtc gccgttggta tcgaacagtt tggcgatctt
     6241 cggatctttc agttgtgcga tgttggtgat tttgtattgg tcggcggttt tcttatcgat
     6301 caggtaaccc tgtgctgcgc cgttaacaaa taccccttca cgataaaatt tcttatcgcc
     6361 accggcagct tcgtacatgt tgtcatggag tggcgtccag ttcacggcgg tgaaggttgc
     6421 atcgccggaa gcaagcgagg tgtagccaac gttgtaatcc acttcgctgg gtttgttgac
     6481 ggtataacct aatttctcca gcgcacgact gaccagcagc gtctggaagg tttcttcagt
     6541 gatggtgctc tgaactggat taacagtaat gcctttgccc ggcagatcgg cagcaaaagt
     6601 ttgtgtagag ataagcgtgg caaacgctgt cgcaaaaagt acgctatgtc gcattgttat
     6661 tccttttttt ggcagggtga taatgcccgg cgtcaccggg caagtgcaga gttacttaat
     6721 gaatgggcgg gtcagcagac caacagggcc agtggtgtac cagcgacggt tgccgcgact
     6781 gcgtgagtcg cgcccaacgg cctgcgtcag gcgatcgagg ataatggcga ggatcacaat
     6841 cccgacgccg ccaacggtgg caagccccat atccagacga ccgataccgc gaagtaccat
     6901 ctgacccagc ccgccgacgg caatcatcga ggcaatgacc accatagaaa gggccagcat
     6961 cagcgtctgg ttaacgcccg ccataatggt cggcatcgcc agcggtaact gaactttgaa
     7021 cagcatctgg cgcgggctgg caccgaatga gcgcgaggct tcaatcagat ccgccggaac
     7081 ctggttaatc cccagaatgg tcagacggat aatcggcggc agcgcaaaga tgatcgtcac
     7141 caccacgccc ggcacgttac cgataccaaa tagcatgacg attggcacca gataaacaaa
     7201 cgctggcgtg gtctgcatgg catcaagcag tggacgaata attttcgccg ctcgcggact
     7261 tctcgccagc catatcccca acggcaaacc gatgacgata cagaacagca gggcagttaa
     7321 caccagcgcc agagtcacca ttgcctgcga acaggcaccg attgcgccga tggcaatcag
     7381 cgagaccagc gtcgccacgc ccattccgac cccggaaatc tgccaggcga tgagagcgaa
     7441 aacgataatc gccactggcg ctggcatacc cagcagcaat tgctggaaac cgttgaggat
     7501 ataatcaacc ggaacgcgca cgccctggaa gacgggacgg aaatgggtaa caacccagtc
     7561 gatcccttca gtgacccaac tgtcgagcgg gatcagcgtt ttatggaacg gatcgagaat
     7621 attaaaatgc tcgacgtttg gcgcaggcgt actggtcagc cagtcagcac caccgccgtc
     7681 agccggtgca gtcgtcggtg tcccccaggc gtctgcggat tgcgcggcac tgtccgccgc
     7741 tggcgtggta tcccacggat tattttgatc agccattatt taccccctca cgatctaaag
     7801 cgcgcagcag cattcctttc gaaatgatgc cgacatactg ttggtcctcg tcgaccacgg
     7861 gcaccgcaca gggtgcctgt ccgacatgag agagcaactc gctaagaggc gtttgtgcat
     7921 cgactgctaa cggcgcatca atcagcgccg catcaagacc ttgctgctgc gttaacgcgg
     7981 ttttaagcga atcgatggag actgcgccga caaacttatt accgcgttcg ataacgtagc
     8041 catattcgcg atcttcatcc tgcaataatt tcagtgccga acgtgggccg aagccaggag
     8101 ttttacgaat taagccattc ggtgtccggc gggcaatatc tttcgcactg aacacctgac
     8161 taatatcaac gccacggaag aaggtacgga cataatcatt cgccggatta ttgagaattt
     8221 catccggtgt gccgacctgt accacttcac cattttgcat aatggcaatt cggtcgccaa
     8281 tacgcatggc ttcatcaaga tcgtgggaaa taaagacaat ggtgcgctga tgtttcgcct
     8341 gtaattttac cagctcatcc tgcatctcgg tgcgaattaa tggatcgagc gccgagaagg
     8401 cttcgtccat taataatata tccggattaa tcgctaacgc gcgggctaat cccacacgtt
     8461 gacgcatccc gccagagagt tcatccgggt agctgtgggc ataattttcc agcccgacct
     8521 gacgcagtgc atcaagggct ttttcccggc gttcttcggc attaattccg gccaattcca
     8581 taccgaacgc agtattgtcc agcacggtca tatgcggcat taaggcaaag gactggaaga
     8641 ccatcgcaat cttttttctg cgcacctcac ggagttcggc gtcggatatt ttggcaatat
     8701 ccacaccatc aatcagcact tgcccgcggg tgggttcaat caggcgattg agaaggcgta
     8761 ccattgtgga tttacccgag ccggataatc ccatgatgac aaatatctcg ccttcttcaa
     8821 tggccagact ggcgtctttt acgccaagcg atagcccagt tttttccaga atttgttctt
     8881 ttgaaagtcc ttgttcgata tatttgaacg ctcgctgtgg atgctcacca aatattttat
     8941 aaagattttt aatttctaat ttaatggcca tgcaatagaa tgattccttt atttgtttat
     9001 gtcgatatat tacccaatgg gaatagtcac ttttttctac cctaacatac tgagaatctg
     9061 aggcaacccc tgagggcaaa tgtggcatga atattgatgc gagcaaatcg ccggaatttt
     9121 ccgtgatata agggctgaga gcaaatcgag aaaagttgat attttttgtc atagatagcg
     9181 aaaaaacgcc tgaatattgt agtttcaggc gaataatgca acagagtatc agtgtagatc
     9241 actacagaat atttgcgtga taaaaagcta tttggcggga attatttccc tgctgcgggt
     9301 agtgatattt ttgaaaataa caccctcaga aattccagtc ttcatcttct gtttcaaccg
     9361 ctttccccat cacataagag gagcctgaac cggaaaagaa atcgtgattt tcatcggcat
     9421 tcggcgaaag cgcggcgagg attgccggat tcacttccgc catttctgcg ggaaataacg
     9481 gttcgtagcc cagattcatc aaagccttat tggtgttgta acagagaaac gctttcacat
     9541 cgtcagccca cggggtttcg gcgtacagct catcggtgta ttgcaactcg ttgtcgtaga
     9601 gttccagcaa caaatcgaag gcgaaactct tcaactcttc acgttgtccc agagatatct
     9661 tttccatgtt tttctgatat ttatagccta tgtagtaacc gtggactgct tcatcgcgga
     9721 taatcagacg gatcaggtcc gcggtattgg tcagctttcc gcggctggaa aaatacatcg
     9781 gcagccagaa accggaatag aacaaaaaag attcaagaaa cacactggcg attttctttt
     9841 tcagcggatc atcaccgcga taatgttgct gaataatctg agcttttcgc tgcaacggtg
     9901 cgttttcttc actccaggcg taggcggcat cgacatcttt ggtctggcac agcgtcgaga
     9961 aaatcgaact gtaagagcgg gcatgaaccg cttccataaa gctgatattc gataataccg
    10021 cctcttcatg aggcgtgagc gcatcgggca tcagcgaagg cgcgccgata acattttgca
    10081 gcgtgtcgag cagcgtcagg ccagtaaaaa cgcgcatcgt cagttgttgt tctacgacag
    10141 ttaatgtctg ccaggcggga atatcgttcg acagcggcac cttttctggt agccagaaat
    10201 tgctggtcag gcgattccac acctccagat ctttatcgtc agatatcttg ttccagttga
    10261 tggcgctgat acgtgagagt ttcatagata ttccttaaag tgcacaggag acgcagcctt
    10321 caatttcagt gccttccagc gccatctgac gcaggcggat gtaatagagc gttttgatac
    10381 ctttgcgcca ggcgtaaatc tgcgctttgt tgatatcgcg agtggtggcg gtatcgggga
    10441 aaaacagcgt cagcgacagc ccctgatcga catggcgagt cgcttccgcg taggtgtcga
    10501 tgatcttttc tgcgccaatt tcgtaagcgt cctgatacag cgccagattt tcgttagtca
    10561 taaacggggc agggtagtaa acgcgtcctg ttttgccctc tttgcgtatc tctactttcg
    10621 ccacaatcgg atgaatactc gacgtagcat ggttgatata agagatagac ccggttggcg
    10681 gcaccgcctg gagattctgg ttgtatatgc cgtagcgcat cacgtcgtcg cgcagttgcg
    10741 cccacatctc acgggtaggt aacgtaatac cgctgcgggc aaacagttcg ccaactttcg
    10801 ccgttttcgg ctgccagtta ccttgcagat attggctgaa atattcacca ctggcatagc
    10861 gtgactgttt gaacccggcg aaggtttcac cgcgttcgcg cgccagcaac atcgaggtac
    10921 gcagtgcgtg ccaggtgatg gtatagaaat agagattggt gaaatccagg gcttccggcg
    10981 aaccataagc gatgccttcc ctcgccagat aaccatgtaa attcatctgc cccagtccga
    11041 tggcgtgcga ggcggcattt ccggcttcga tggacggcac gctgcggata tgactcatat
    11101 ctgacaccgc cgttaaaccg cgcacggcag tctctaccgt gcgggaaaaa tcgggggaat
    11161 ccatggtgtg cgcaatattc aacgaaccta aattgcagga aatatcatgg cctgtgcggg
    11221 catagtcgag attctcgtca tactctgagg cgctgttaac ctgcaaaatt tctgagcaga
    11281 gattactcat atttatgcgc ccggcgatag ggttagcacg gtttactgtg tcttcataca
    11341 tgatgtaggg atagccggac tcaaactgga tttctgccag tcgctggaag aaatcacggg
    11401 cgttgaggta ttttttgcgg atgcgttcat cggcaaccag ttcgtcatag tgttcgctaa
    11461 tggcgatatc ggcaaacggc ttgccataaa ctcgctcaac gtcatagggc gaaaacagcg
    11521 ccatctgcgc attctctttt gccagatgga aagtgatatc cgggatcacc acgcccagcg
    11581 acagtgtttt aatgcggatt ttttcgtcgg cattttcccg tttcgtgtcg agaaaacgca
    11641 gaatatcggg atgatgagca tgtaaataga ctgcacctgc cccctgacgc gcgccgagtt
    11701 ggttggcata ggaaaatgcg tcttccaaca ttttcatcac cggaattacg ccagaagatt
    11761 gattttcaat acgtttaatt ggcgcgcccg cttcccgcag attcgacagc aaaaatgcta
    11821 cgccgccgcc gcgtttcgac agctgcagtg cggaatttac cgcccgacca atcgactcca
    11881 tattgtcttc aatacgcagc aaaaaacagg aaaccagttc gccgcgctgc tgcttaccgc
    11941 agttgaggaa tgttggcgtg gctggctgaa agcgtcctga cagcatttca tcggtcagtt
    12001 gcaacgccag cgtctcatcg ccttgtgcca gcgtcagcgc caccatcgtg actcgatcgg
    12061 caaaatcttc cagataacgt ttaccgtcga atgtcttcaa cgtatagctg gtgtaaaact
    12121 tccatgcccc gaggaatgtc tggaaacgaa aaccgctggt gtgtgcgtgg gcaaacagcg
    12181 taatgacaaa gtcgcgagag tagcgattaa gaacgctttc atcatagtaa ccttcgttga
    12241 ccagccagtt caggcgctgc tgctggctac tgaaggtcac actgttcgga cgcacatgcg
    12301 tcgcaataaa ggcgtcaacg gcctggcggt ctttatcgaa ctgaatgcga cctgcgctat
    12361 cgtaaagatt aagcatcgca ttcagcgcgt ggtaatccat cgtttcctgc gtcaggcatt
    12421 ctgcggttgt cgttgccaaa attcggttac tcctttacga acgttttcga tatcgctttg
    12481 cgtacccatg agttcaaaac ggtacagcca cggcacgccg catttccggg caatcacatc
    12541 tccggcgcgg ccatacgcct caccaaagtt gcgattacca gaagcaataa cgccgcgaag
    12601 caacgcccgg ttgtgctcgt cgtttaaaaa gcgaattacc tgtcgtggca ccgcgccagc
    12661 cgtaccgccg ccgccgtaag agggcacgat caggatgtaa ggctcgtcta cctgaatccg
    12721 ttcccgctca ttgagcggga tgcgcaccgc gggcagacct aaacgttcga taaaacgctg
    12781 cgtgttttcg gagctgctgg agaagtagac gagctggctc atgcactggc cgcgtgtggc
    12841 gctggatgca gacggttaat catgtccgga cggaaaccag accagctaag atcgccagca
    12901 atcactaccg gcaactgacg aaagccctga gcacgcaacg cttccgctgc ttcaggaacg
    12961 cgatcgacat taatcatttc aaaatcaaag ccccggtttt ccatcgcccg tttggtggcg
    13021 tggcactgaa cgcaatcgtt acgagtataa atagttatgc gcatgattcg tatttctgtt
    13081 taaaatgaag atacggcgcg atgatacgcg tcgggttgtc tctctgttga tacagagata
    13141 ctagatgtag ttgaaaaaag attcaaccac acaatatata gcaaatagtg atcgaaatta
    13201 ccctggatat gagcgtgacg ggggaggggg atttttgtga ttcaccaggc aaaaagaaac
    13261 cccgaagaca ggcttcgggg tcaaagacgc gtatttatta tcatttttgc actacgattt
    13321 gcgcatgctt aactgtgcgc cgataaaaat acctaccgca gctgctgtac ccacgctaca
    13381 ccagggtctt tcacgaacaa aagaatccgc gcagccaacg gcatcgcgcg cggcttgctg
    13441 gacgcgagta cgaccatgca ttcgcgccct ggtttctttc agcaacgcct gagctttact
    13501 gcgtgcggct tcagcttccc ctttggcgtc gctgccccag gacttcaata cagattcgag
    13561 gctgtcagct aattgattga catcattctg gatatcctgc acgccatcat caacgtcgtt
    13621 gcggttcggt cggttaaaca tatgatcccc cattagaatt cgatgtaatt ttcagtgtag
    13681 accataattt tcttaactga ggttcatcgc ggctttatgg acttttctga aagcgttgtg
    13741 aatgcgcaat caggtaaggt aggcccgcgg ttgaaaataa cgaggatcaa atatgtattt
    13801 acgaccagac gaggtggcgc gcgtacttga aaaagtcggt tttactgtcg atgtggtaac
    13861 ccagaaagcg tatggttacc gccgtgggga aaattatgtc tatgttaatc gcgaagcgcg
    13921 aatgggacgt accgctctgg taattcatcc aacattaaaa gaacgcagtt caacactggc
    13981 tgaacccgct tccgatatta aaacttgcga tcattaccag caatttccgc tctatttagc
    14041 aggcgagcga cagcgacacg agcattacgg tatcccgcat ggctttagtt cgcgtgttgc
    14101 gcttgaacgt tatttgaatg gtttatttgg cgaagccagt taaagacgcg actggcgatg
    14161 ccagtcgcga aaagaagagt caggctttta cctgctggta acggctgact ttaaacagtc
    14221 ggcggcaata atcgaggaag tagccataaa ccgcccccat caacatcgaa acaacgatgt
    14281 ttgaactgac cgccgccata atctgatgcc agtctgcgcc caccactaac aagatcgcca
    14341 catacaccgg tgactggaac gtcacataag ccaggatatc agccagattt tttatccagc
    14401 ctttgcgtgc cgcgcgcata aacagatcgc ggtacatacc gtatggccat gcaattaaga
    14461 tgttcaccgg aatcgctacc aatctggaat aaaaagactg ttcgaagctc attccggaga
    14521 ggaaaacttc aatacacatg ttcacgacag aacagtaaac aaccatcgcg aacgtatctg
    14581 caactgcatg acgcaagcgt gactgcggtg agaacatgct aatgctcctt aataaaagcg
    14641 taatgggatg ttaatggaga tgaaagcagt ggatctcatt gccatctgta ttttcgcata
    14701 gcgtatatcg ctggctatga ctaataaatt agtgaaaaat ttttatttag tcagcttttg
    14761 tagataaaat attccgtaac ggctcgtttt ttgtacattg tggattttta catgccgata
    14821 tgatttattt tcaattaaat caattggata tggccttgcc tttaatttca ctaatgaaag
    14881 tttggggggg aggtaacgct ataagcgtag agaattcggt gtttgtcgtg accgggtcat
    14941 ttacagcatt tttgatgcca gaaaatgtac attaacagca gaaagcacca gtgaggggtg
    15001 ttttctttta atctgttata ttgcattttt attcacttcg gttttgtcag gcttacggag
    15061 ttagcgagca gagagcgcca ctctgttcac caataaccta aaatactcat caaaatggct
    15121 aatatataat cttgaaatta ttctgagagt ttcagaaaat aacagctgaa ataatctcgc
    15181 gcaggactgt aaatagatta aattttgtgg aaatataata agtgatcgct tacactacgc
    15241 gacgaaatac ttttttgttt tggcgttaaa aggttttctt tattatgtcc gtaatgttac
    15301 aaagtttaaa taacattcgc accctccgtg cgatggctcg cgaattctcc attgacgttc
    15361 ttgaagaaat gctcgaaaaa ttcagggttg tcactaaaga aagacgtgaa gaagaagaac
    15421 agcagcagcg tgaactggct gagcgtcagg aaaaaattag cacctggctg gagctgatga
    15481 aagctgacgg aattaacccg gaagagttat tgggtaatag ctctgctgct gcaccacgtg
    15541 ctggtaaaaa acgccagccg cgtccggcga aatataaatt cactgatgtt aacggtgaaa
    15601 ctaaaacctg gaccggtcag ggccgtacac cgaagccaat tgctcaggcg ctggcagaag
    15661 gtaaatctct cgacgatttc ctgatctaat tactgaggcc ggatgtcgct gctagggcgc
    15721 gtccggcgtc gcttctcaag ctcgttggtt ccacggtatc ttatctaaca accttgccat
    15781 tccacaaaaa ccgctgattc ctgcaaacag taacccggca ccaacaaagc cacttaataa
    15841 gaagaaaccg ctatttacgg tataacccag tacaacgccg attaatatta aaccacccgc
    15901 agcgatctgc acctggcgca ttaacggcaa gggttgagat ttatttaccg ctactggcaa
    15961 tccagctctt ttccagccat caatcccatc ttcgagtaaa aatatttctg cgggggcggc
    16021 aatcgctgct aatttatcgg cgttattact ggtgcgttta cctgcctggc agtggaatat
    16081 aatttgctcg cgacgtaatt taggtggaag acctgactgt tccagcacgg ataatggagc
    16141 cagatctgct tcaggaatat gttcacgaag atattcatca gcgtcacgaa tatcgattaa
    16201 ctttgcgccg cgtgcgatta attcttgtgc atcatgcggc gaaatggttg tcaaagccat
    16261 agtgactcct tacggacaat agacattttt cagggtggcg ataatggcat ttaccgcctc
    16321 atttttaatg gaatatagaa tgcgttgggc atcccgttgg ctgttgataa gcccttcgtc
    16381 ccgcatacga gcgagatgct gtgatgtcgc agaggcgctc agtccggtaa tgcgcgtcag
    16441 ctctcccgcg ctggtgccgg gggaaccgct aagcatgcac agaatcagca accgtttagg
    16501 gtggctcatt gctttcaata aggccgctgc ctgttcggta ctggcctgta attgcgcgag
    16561 ttcagtcata tttatttaag tattttctaa attaagtaaa ctctaaacta aaaatgcaac
    16621 atataccagc ctcagcagcg taaatgagag taaaagcgta agctgaaact ggcaggctcc
    16681 gctaaaatta ctacgcttaa gagataaaat ctctttttaa acaatgagta attttcttat
    16741 agggagtaca tatgggtttc tggagaatcg tcatcaccat cattctgccg ccgctcggcg
    16801 tgctgctcgg taaagggttc ggttgggcgt tcattattaa tattctgctg acactgcttg
    16861 ggtatatccc cggtttgatt cacgcgttct gggtgcaaac ccgcgattaa acctgtgctc
    16921 acccccgcgg cgttaacgcg ccgcgttcct ctgctacact ttctgagtgt ttccgttaac
    16981 gaatgaggta gctatgggtc tgttcaattt tgtgaaagat gccggagaaa aactctggga
    17041 tgcggttaca ggtcagcacg ataaagacga tcaggcgaag aaggtgcagg agcatctgag
    17101 caaaaccggt atacctgatg ccgataaagt gaacattcaa attgccgacg gcaaagcgac
    17161 ggtcactggt gacggcctga gtcaggaggc gaaggagaaa atccttgttg cggtggggaa
    17221 tatttccggt attgccagtg ttgatgatca ggtgaaaacg gcgacaccag ccactgccag
    17281 ccagttttat accgttaagt ctggcgacac tctgagtgcc atttccaaac aggtctacgg
    17341 taacgctaat ctgtacaata aaatcttcga agcgaataaa ccgatgctaa aaagcccgga
    17401 taaaatttat ccggggcaag tgttgcgtat tccggaagag tagttaattg ccagccatcg
    17461 cctgctggat aatgggaatt ggcgtcagta aatgctggcg cattaactca ctgacgcgcg
    17521 ctgtatctcg cgccagtacc gccgcagtca gggtctggtg ctgatcgtgt ttatcctcca
    17581 gcatttccac cgaaagcacc gttcgccgca gccagataaa tcgataacgc gccgccagat
    17641 caaacaaccg ttcacgcatt tgcagcaaat agtgagaacc acagcccgcc acaattgctg
    17701 tatgaaacgc ctgatgacgc agatcccact catcaagcat tttctcgctg gcgtcacagg
    17761 cttccagctt attgagcaga tgcgcttttg cgagaacgtc tgcctcccat tcatcgccac
    17821 cgcgggcaat cgccagactg accagcatcg cttccatatc gggtggcgtc tatgtcagag
    17881 cagctcctgc tctgacatag acgccacccg ataccctttt tgattcacca ccgtgaccag
    17941 ccgttccgcc accagttgag aaagtgcttc ccgcaacggc ccaacgccaa gtgcataacg
    18001 cgatgtcagt aaactcatcc gtaatttttc atccggttga aaattaccgc gaataatatc
    18061 gttcttcagc cagcgatagc catccagaga cgtaatggtc atggtgtgac tcctgatcat
    18121 tatcagcgcg tattatgaac gggtgttttt tgccacaata ccagcttttt ccagcgagcc
    18181 ataattggca cggtacagat aatccctatc gctaataagc cggtggagat cacttctaac
    18241 tgttgcgccg gacggaatag catcactacc aacacaaagg taataaagcc gatgaccagc
    18301 caggtgagcc agggataaag ccacattcgc aagcgaattt cgcttccttc tgctcgcaga
    18361 attttgcgca tccgcaactg tgaaacggcg atgactaaat aaaccagcag ggcgatagcg
    18421 ccggagctgt cgatcagaaa tttaaatacc ttcgcaggcg cgtaatagtt caccaccacc
    18481 gttaagaatg ctgcgccggt ggagagtaac accgccacgt acggggtttt actgcggttg
    18541 attttaccca ttaccgcggg agcatcaccg cgacggctta aggagtagag catccttgag
    18601 gcggtataca gggccgagtt caggcaactg gttacggaaa gtaatatcac acagtccatg
    18661 attaatttcg catggggaat attgagcaat tccagaaccg agcgataaga tccaacggct
    18721 ttcagccccg gc
//
LOCUS       DADLTF010000076        18264 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.076, whole genome shotgun sequence.
ACCESSION   DADLTF010000076 DADLTF010000000
VERSION     DADLTF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.076"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(66..824)
                     /gene="aroD"
                     /locus_tag="JN749_002446"
     CDS             complement(66..824)
                     /gene="aroD"
                     /locus_tag="JN749_002446"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAY7368825.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="JN749_002447"
     CDS             complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="JN749_002447"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HAY7368826.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1733..2947)
                     /locus_tag="JN749_002448"
     CDS             complement(1733..2947)
                     /locus_tag="JN749_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368827.1"
                     /translation="MYFSYFLHGISVITLAQNMTSLAEKFSTDNAGIAYLISGIGLGR
                     LISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLTLAYGLAVCVGIANSALD
                     TGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYMLLNNIWYGYGLIIPGILF
                     VLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSSVLFGVAAFSTFYVIVVW
                     MPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKMVRPIWANVFNSALATIT
                     AAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFPKSKAKVTSIYMMMGGLA
                     NFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRYYRVFIIPENDVRFGERK
                     FSTRLNTIKHRG"
     gene            complement(3187..4331)
                     /locus_tag="JN749_002449"
                     /pseudo
     CDS             complement(3187..4331)
                     /locus_tag="JN749_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707589.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4490..4846)
                     /locus_tag="JN749_002450"
                     /pseudo
     CDS             complement(4490..4846)
                     /locus_tag="JN749_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4982..5089)
                     /gene="rprA"
                     /locus_tag="JN749_002451"
     ncRNA           complement(4982..5089)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="JN749_002451"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5275..6387)
                     /gene="ydiK"
                     /locus_tag="JN749_002452"
     CDS             complement(5275..6387)
                     /gene="ydiK"
                     /locus_tag="JN749_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HAY7368828.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAILAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6776..9832
                     /locus_tag="JN749_002453"
     CDS             6776..9832
                     /locus_tag="JN749_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAY7368829.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVVLIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9829..10239
                     /gene="menI"
                     /locus_tag="JN749_002454"
     CDS             9829..10239
                     /gene="menI"
                     /locus_tag="JN749_002454"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HAY7368830.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10339..10527
                     /locus_tag="JN749_002455"
     CDS             10339..10527
                     /locus_tag="JN749_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368831.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11075..11443
                     /gene="sufA"
                     /locus_tag="JN749_002456"
     CDS             11075..11443
                     /gene="sufA"
                     /locus_tag="JN749_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HAY7368832.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11452..12939
                     /gene="sufB"
                     /locus_tag="JN749_002457"
     CDS             11452..12939
                     /gene="sufB"
                     /locus_tag="JN749_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAY7368833.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12949..13695
                     /gene="sufC"
                     /locus_tag="JN749_002458"
     CDS             12949..13695
                     /gene="sufC"
                     /locus_tag="JN749_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAY7368834.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13670..14941
                     /gene="sufD"
                     /locus_tag="JN749_002459"
     CDS             13670..14941
                     /gene="sufD"
                     /locus_tag="JN749_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAY7368835.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14938..16158
                     /gene="sufS"
                     /locus_tag="JN749_002460"
     CDS             14938..16158
                     /gene="sufS"
                     /locus_tag="JN749_002460"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HAY7368836.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQAQGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16171..16587
                     /gene="sufE"
                     /locus_tag="JN749_002461"
     CDS             16171..16587
                     /gene="sufE"
                     /locus_tag="JN749_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HAY7368837.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16736..17740
                     /gene="ldtE"
                     /locus_tag="JN749_002462"
     CDS             16736..17740
                     /gene="ldtE"
                     /locus_tag="JN749_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HAY7368838.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17804..18040)
                     /locus_tag="JN749_002463"
     CDS             complement(17804..18040)
                     /locus_tag="JN749_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HAY7368839.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4811 a         4403 c         4689 g         4361 t
ORIGIN      
        1 aaatacggtt aaaaatactt tttttatttc ggcgggggag ggtatttccg ccgaaatatt
       61 attgcttatg cctgatgtaa aatagttaat accgtgcgca aatcatttac cgagatttgc
      121 cctggcgcag aggctttttt taccgcacca aaagttgccg ccgagccaaa tacttcacca
      181 gccagacgag aaattacgcc agtttttgcc atcgacatcg tgatgattgg acgatcggca
      241 tactgctctt gcatctccag ggtcgcggca agcaacgtca gcacatcgct ggtactttgc
      301 ggcatcagcg caatcttagg aatatcggcg tcgaaggact gcattttgcg cagacgggca
      361 atgatttctt cggcttccgg cgttttatgg aagtcatggt tggacatgac aactttcaca
      421 tcatgcgcgt gggcgtaggc gacggtttct ttgacctgat catcgccggt aaataactcc
      481 agatcgatca tatcaaccag gccgctgtcg atggctgcac gattgagagc aatataagcc
      541 tcggtggaaa tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa cagcagcggt
      601 ttttctggca tggtttcacg gagaattttt gccgccgcca tgacagactc cacattggag
      661 aggtcggcaa agtggtccac acgccattcc agaatatcaa agtccgcttc acgataggcg
      721 agagcttcgg atttcacgcg ggcgatatct ttcgccatca gcgagacgat gattttaggt
      781 gcgcccgcac caatgacgag atcttttaca gttacggttt tcatttttta ccctttctgc
      841 acgcggtcag cctgtcaggc accgaacccc atgacctgtt taacatattc cagagggaaa
      901 tctttgccag tccataacgt gaactgttca gccccttgcc acaacaacat gccgtatcca
      961 tcaatcgttt tgcaaccagc ttgttgtgcc tgctgcaata acttcgtcat atgcgggtta
     1021 tacacgcatt cagtgaccag cagtcccgga tgtaacagac tgatatcatt aaccaatgat
     1081 tcattctcaa ggggtttcat acctactttt gtgccattgg ttaaaatgtc ggcggaagcc
     1141 agggcttcag caaaggcttg ctgatcggcg agatcggtaa ccgtgacgac acaatcggtg
     1201 ttttcgttaa cccgctgcgc gaaggcgagg gctttatcga agaactcatc ccgacggtta
     1261 aagagtttaa tttcttttaa accttcaatt gccccctgcg cgccaatcgc cgttgaggca
     1321 cctccggccc ctaacagcac catcgttttg cctttgatat caaaaccgct ctctttaatg
     1381 gcgcgaatat ggcccgtgcc gtcggtgtta tagccacgca gatagccatc atcattaacg
     1441 atggtgttga tggcacccac cagtttggct gctggcgtta attcatcaac atattcacac
     1501 gccagttgtt tgttcggcat cgacacacca gttccgcgca ttttgagggc ttttaatcct
     1561 tcaattgctc cgggaaagct atcgttatcc acttcgaagg ccatataggt aaatggcaat
     1621 cccgcttttt ctaaggcttt attctgcatt tcaggcgata aactgtggcg gataggataa
     1681 gccatcaatc caatcaattc gtattttgcg gtaacatcca taattaactc ctttaacctc
     1741 tatgcttaat tgtgtttaac cgggtgctaa atttgcgttc gccaaaccgc acatcattct
     1801 caggaataat aaaaacgcgg taatagcggc taaaaacaat aattgcggta atcagggcca
     1861 gcagcgcgaa agtaaaatcg agaacaatga tatattgcag gccgatgttc gacagataac
     1921 cggtaatcag cggaataaca aagttagcca gtccacccat catcatataa atactggtga
     1981 ctttggcttt gcttttgggg aaaaactctg acatgaccga aacaccgagc tgtaaaatgc
     2041 cgccagctgc tgagaaaccg ataacaaagg cgccggcatt acacaccagt ggagaagggt
     2101 acaggtagat aatggcagct gttattgttg ccagcgcaga gttaaataca ttagcccaga
     2161 tgggccggac catttttttc agtagtgcgg caaaaataaa gacacagacc aacgagccca
     2221 tactgtaata agagatggtt tttaatgcct cagcttctga cataccagca aaagccatcg
     2281 catatttggg catccacacc acaatcacat aaaaggtcga gaatgcggct acaccgaaca
     2341 gtaccgatga aacaccttcc agccagacta acggtttgct gttcatttgc ggtaattcat
     2401 tagctacgct ggcgtccacc aactggctgg ggaatttgct tttcaacagc atcagcgtga
     2461 tcagtacaaa cagaataccc ggaataatca gcccatagcc gtaccagata ttattgagca
     2521 acatatagct caccagcatt gggtagaaca tttgcccaaa tgacaccatc gctttaacca
     2581 gtatgaccgc cgaaccagag gctttcggaa agcattccat gagcgcgggg tagccacccg
     2641 tatccagcgc tgagttagcg atacctacgc acactgccag accgtaggcg agagttaaat
     2701 tcgggcaagc gggaatacca aagaagaata gcagatacat tattactgcc attaatatca
     2761 ccgcccgacg accaaactta tcggagatca caccgaagaa taaaatactg atcaatcgcc
     2821 ccaaaccgat accggaaatt aaataggcaa tgcccgcgtt gtcagtggaa aacttttctg
     2881 ccagagatgt catattttgg gcaagcgtaa taacactaat gccgtgcagg aaatagctga
     2941 agtaaataca aagaacagcc aggataaatg gcgtgctgaa agataatttc gttaccgtac
     3001 gtattgttct ccgacggttt acataattac tatactgaag atgattttcc atgggtacat
     3061 ggcataaagt ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt
     3121 tccttagtta gcgcagaaag agttatttat ccgtaagatc ttgcatgacc tgaaaagaat
     3181 attgcattac ccacccggag cgacattttc ctttagcgag tgatgccgcg ttttttgcgg
     3241 ctgcgtaagc cgataaacag tgccagtaaa aaaccgatgg cagtatcgaa ccacataata
     3301 tcggcaatac ttctttggga cagatgagcc gtaatcaacg gaatagtaaa ggtcgcaata
     3361 ctgcccgcac tgtaatagat ccctgtagct ttacctttag cgtaagggaa acgttcagct
     3421 attaacgtca ggccaatttg cacaacacct ccagcagagg taaaaccaat gacaaaagca
     3481 aatattatca ccacataaaa tgtgggatgc aggcagacgg taaacagagc aataaatgag
     3541 ataaaggtgt acagcatcag taatgttgtt gggcgaacgg tattacgaat gagtggagcg
     3601 gtaataaata cacaaagcag cgaacccacg gtgtagatac tgagtagttt gatcgacata
     3661 gtgtatgaca tgcctgcaac aaattgtccg tactgtgcca gccactggct aaccagataa
     3721 aacgttgcca ttgagatata gccatataag gtataactgg ctaaatcgat aattgaacag
     3781 cgatgttccg tagagctggt ggttttcttt atgacaggta agcgacgacc cggatggggt
     3841 gggaacgtac aacggtataa aaacagagcg ttaataaaca taatgcctgc agcaatcata
     3901 aaggaccaac cgaaccacag ttcagcccac actaacaggc taatgattag cggtaataaa
     3961 aattgtccgc tggaaacaaa tgctttaatt aaaatattgg ctgtcccagg tgagcgtgga
     4021 aaagcttcca tcaaactggg ataagtgcct gcatcgagaa aactgtttgc cattcccgcc
     4081 agaaagccaa aaacataagc gataatgatg ttattggtct gcaggatgcc aaaaaagaag
     4141 gccatatagc agcacattcc gagcatgata aaagggcggc gaccaaagcg atcggataat
     4201 aatcctgcaa aaagcaagac acttaatcga ccaatgccca gcgatgagat aactatcgag
     4261 acacccgcgg cattagtctg acaaagtgtc tccagcgagg ccatattcag gctcatcaaa
     4321 atgacgccca taccatgcac caggtaataa aatacaaccc aagtgcggta gggtaatagg
     4381 gatttttcat aggttctact tagcaaaggt gctttaaaaa gtgtagcgat gttgtgtcag
     4441 tatccattca ttattgttgt aacaacgaca attcctttct gtggaaaaat taacaaagcc
     4501 gctccagatc gtgtgcatat aattcagcgg ctaccgattg atagatcttc cagtcaataa
     4561 aattgccatc agggtagact tgttgaaacg cggttaagtc ggggaaaaaa cgcatggtgt
     4621 tgttgccgat gcggttattt attttctcta tgatggcatg aagatgtttt tttcgttact
     4681 gacgcatagc caacagttgc tcgactacac aatcaggaat ggcgcatttg ccattttccc
     4741 actgacgcca gctttcactg tcaccggtct gggcaatcca ggtcgcgcat tcatcaatag
     4801 tcatggcaaa aatatggcgg agtgcctgga gttcataagc gttcatcgtg taatggctgc
     4861 atgaaataaa caataaaata taaataacat tatcagcctg ctgacggctt gaagagagtc
     4921 acagtatctt gtgcaacatt attggaaaga gtgaggggcg aggtagcgaa gcggaaaaat
     4981 gttaaaaaaa agcccatcgt gggagatggg caaagactac acacagcaat tcgttgtttc
     5041 actcagggga tttccatgct tataaatcaa tatgttgatt tataaccgtg agctaatagt
     5101 aggcatacgg actttttgcg tcgatcagat tcgtctcatt agtttaaaaa ctgtaaagaa
     5161 tttgcgacag gtactaccag ctgaatggcg gtaagcagga aaatatgggg aagagcaagc
     5221 aaatatcaat tagcggtgat taacaatcag gcggcagagc cgcctgatga gaaattactt
     5281 attcggtttc tcgatttcgc caagttcttc gagaatttct tccggttggt ccgtcggcgg
     5341 cgggacttct tccacccacg cggcaaacag acgccaggaa acggctaaca gaaccggacc
     5401 aataaacaga ccgatcatcc cgaaagcaat caaaccacca ataacgccag agaggatcag
     5461 gatcagcggt aaatcggcac ccatgcgaat taacattggg cggatgacgt tatccagcgt
     5521 gccaaccaca ccgctccaca ctaacaatac cgttccccag gtggtatcgc cggtccagta
     5581 gagccagata atcgccggaa tcagtaccgg caacgggcca agctggacaa ggcaggagag
     5641 gatcattaac accgttagca gcgttgcata aggtacgccg gatacggcga ggccgatacc
     5701 gccaagcact gcctgtacta acgccgtcac caccacaccc agcgccaccg cgaggatagc
     5761 ctgtgccgcc agcagcacgg cggcatctcc gcgaacgcct gccagacggg ttgcaaaatg
     5821 gcgaatgcct tgtgccacct gttcaccgcg ccagtacagc agggcactga agagaagcat
     5881 cagcgcacaa tgcaccataa agcgcccgat atgcgccgcc tgcccaacga accaagtggt
     5941 ggtggtgcca atataagggc ggactttcgc catgatcgcc gtacccccca tatccagcaa
     6001 gttgtgccag cctgcataca gcttcgcgcc aatcaccgga atggtattaa gccacgccag
     6061 atcgggtaac gtcatgtcgc cgctggaaat ggctttaatt agcgggccgc tgccgtcgac
     6121 gatactgtta accagcaaag caatagggat gataaacacc atcactaata acagcgtcat
     6181 caccagaacg gcgagagagc ggcggccaaa catgatcttt tgcaaacgta acaataccgg
     6241 ccaggtggcg ataaccaccg taccggccca tgcaaagccg agaataaagg gttgaacaat
     6301 ccacagacat gccacaatca tgatggctaa aaacagcacc gaaagcagaa tttgtgcgac
     6361 atccctgggc tgacgaacat ttaccataac tacttttcac cttttttgtg cgtcaaatcg
     6421 tcgacgcgac gtggacacgc gaaactcacc tgcataatga tgagcaattt cagcggtttt
     6481 taacaggccg attctgtcct taattctgat gggcgtgtta gaaaaaaatg tggtacaaca
     6541 actcaggcaa cacgcaaacg atttactcgt cgtatttcaa gttgcatgtg ctgcggctac
     6601 gttcgttcac cccagtcact tactattgta agctcctgga gttcactcgc ttgccgcctt
     6661 cctgcaactc gaaatccata gagtaaataa acatcacctt tcagtgcgct attgcagccg
     6721 cctgaaagaa tagtgtatcg acctacatca cagaccgcag gaaagggtca atataatgat
     6781 tccacagatt tcccaggcac ccggcgtcgt tcaactggtg cttaattttt tgcaagagct
     6841 ggagcaacaa ggttttaccg gcgatgcggc gacaagttat gccgatcgtc tgacaatgtc
     6901 gaccgacaac agtatttacc aacttctccc cgatgcggtg gtatttccgc gttcaaccgc
     6961 agatgtggtg ctgatcgccc gtcttgccgc gcaggaacgc tattcatcgc tgatctttac
     7021 cccccgcggc ggcggcaccg gcactaacgg tcaggcgctc aaccagggga ttattgttga
     7081 tatgtcccgc catatgaacc gcatcatcga aattaaccct gaagagggct gggtgcgcgt
     7141 tgaggccggg gtgataaaag accaactcaa tcagtacctg aaaccgttcg gctacttttt
     7201 tgcgccggaa ctttcgacca gcaaccgggc aacgctcggt gggatgatca ataccgatgc
     7261 atccggtcag ggatcgctgg tctatggcaa aacgtcagat cacgtacttg gcgtacgcgc
     7321 ggtgttgttg gggggcgata ttctcgatac gcaaccttta cccgtcgagc tggcggaaac
     7381 gctgggtaaa tccaatacca caatcgggcg aatttataac acggtttatc aacgttgccg
     7441 tcagcaacgc cagttaatca tcgacaactt ccccaaactt aaccgctttc ttaccggtta
     7501 cgatctgcgt catgtcttta acgatgagat gaccgagttc gacctgacgc gcattctgac
     7561 gggttcagaa gggacgctgg cctttattac cgaagcgcgg ctggatatta cgcgcttgcc
     7621 taaagtgcgc cgtctggtga atgtcaaata tgactctttt gactctgcgc tgcgtaacgc
     7681 gccgtttatg gttgaggcgc gggcgctttc ggtagagacg gtggactcaa aagtgctgaa
     7741 tctggcgcgg gaagatattg tctggcattc cgtcagcgag ttgattaccg atgtgcctga
     7801 caaagagatg ctcggactga acattgtgga atttgctggt gatgatgagg cgctgattga
     7861 cgagcgagtt aatgcactct gtgtgcggct tgatgagctg attgctagcc aacaggcagg
     7921 tgtgattggc tggcaggtgt gccgcgagct ggcgggggtt gaacgtatct atgcgatgcg
     7981 caaaaaagcc gttggtctgc ttggcaatgc caaaggtgcc gctaagccaa ttccgtttgc
     8041 tgaggatacc tgcgtaccgc cggaacatct ggccgattat attgctgaat ttcgcgcgct
     8101 gctcgacagc cacggcttaa gctacggtat gttcggtcac gtcgacgcag gtgtcttgca
     8161 cgtccgtccg gcactggata tgtgcgatcc gcaacaagag attttgatga agcaaatctc
     8221 tgatgacgtg gtggcgctga ctgcgaaata cggtggtttg ttgtggggcg agcacggcaa
     8281 aggttttcgc gctgaataca gcccggcgtt tttcggtgag gaactttttg cagaactgcg
     8341 caaagtgaaa gcggcatttg acccgcataa ccgactcaac ccagggaaga tttgcccgcc
     8401 agaaggtctc gatgcgccga tgatgaaagt ggacgcggtg aagcgcggta cattcgatcg
     8461 gcagatcccc attgcggtac gccagcagtg gcgcggtgcg atggagtgta acggcaacgg
     8521 tttatgcttc aactttgatg cccgtagtcc gatgtgtccg tcgatgaaga tcacccagaa
     8581 ccggattcat tcaccgaaag ggcgcgcaac gctggtgcgt gaatggctgc gcttgttggc
     8641 ggatcgcggc gttgacccac tcaaactgga acaagaactg cctgaatccg gcgtcagttt
     8701 gcggacgtta attgcccgca cgcgcaatag ctggcatgcg aataaaggcg aatatgactt
     8761 ctcacacgaa gtcaaagagg cgatgtcggg ctgtctggcc tgtaaagctt gttcgaccca
     8821 gtgccccatc aaaattgatg tgccggagtt tcgctctcgt tttctgcagc tctatcacac
     8881 ccgttattta cgcccgcttc gcgaccacct cgttgctacg gtcgagagct acgcaccgct
     8941 gatggcacgc gcgccgaaaa cctttaactt cttcattaac cagccgctgg tgcgcaaact
     9001 ctcggaaaaa catatcggca tggttgattt gccactgctg tcggtcccct cgctacaaca
     9061 acaaatggtg gggcatcgct cggcaaacat gacgctggaa cagcttgaag ctctcaatgc
     9121 agagcagaaa gcgcgcacgg tgttggtggt gcaggacccc tttaccagct attacgatgc
     9181 gcaagtggtg gcggattttg tccgtctggt tgaaaaatta ggtttccagc ctgtgttact
     9241 gccattttcg ccaaatggca aagcccagca tatcaaaggc tttcttaatc gttttgcgaa
     9301 gacggcgaaa aagacggcgg atttcctcaa ccgtatggct aagcttggga tgccaatggt
     9361 gggcgtcgat ccggcgctgg tactttgtta tcgcgatgaa tataaactgg cgctgggcga
     9421 ggaacgtggc gagtttaacg tcttacttgc gaatgaatgg ctggcaagcg cacttgagtc
     9481 acatccagtg gctacagtca gcggtgaatc atggtatttc tttggtcact gtaccgaggt
     9541 taccgctttg ccgggcgcgc cagcacaatg ggccgcgata tttgcccgtt ttggcgcgaa
     9601 actggaaaat gtcagcgtgg gttgctgcgg catggcaggg acttacggac atgaagcgaa
     9661 aaaccatgaa aattcgctcg ggatctatga gttatcctgg catcaggcta tgcagcgact
     9721 gccgcgtaac cgctgtctgg cgaccggata ttcctgccgt agccaggtaa aacgggttga
     9781 aggcacgggg gtacgccatc ctgtgcaggc tttactggag attattaaat gatatggaaa
     9841 cggaaaatca ccctggaagc actgaatgct atgggtgaag gaaacatggt ggggttcctg
     9901 gatattcgct ttgaacatat tggtgatgac acccttgaag cgacaatgcc agtagactcg
     9961 cggacaaagc agcctttcgg gttgctgcat ggaggagcat ccgtggtact ggccgaaagt
    10021 atcggttccg ttgccggtta tttatgtacc gaaggtgagc aaaaagtggt tggtctggaa
    10081 atcaatgcta atcacgtccg ctcggcacga gaagggcggg tgcgcggcat atgtaaaccg
    10141 ttgcatctcg gttcgcgtca ccaggtctgg cagattgaaa tcttcgatga gaaagggcgt
    10201 ttgtgctgtt cgtcacgatt gacgaccgcc attttgtgac gttgtcacca gaaaagtgtg
    10261 acgcatattg cttttggtca ttatctgcac agtaaacggt ggtatactgt gcaggtcttg
    10321 tctaaaagcg aggatgctat gtctactcaa cttgatccca cccagctggc cattgaattt
    10381 ttacgccgtg accagagcaa tctttcacct gcccaatatc tcaaacgtct gaaacaacta
    10441 gaacttgaat ttgctgacct gcttactctc tcttctgctg aattaaaaga agaaatctac
    10501 tttgcctggc gtctgggcgt gcattgatcc tgacttaatg ccgcaagttc gccaaatcga
    10561 cctggattaa cctccgtcca ggttcgatga ccttcttaat actgcttatc taccgcctga
    10621 tattgttcaa aaataatact aatcgcagtt tgtgttaaaa cggcgggtta gctttatgag
    10681 attattctta tcgccccttc aagagctaag ccactgagag tgccggagat aagcgccgga
    10741 tggggtagaa acccttaagc ctgtgtcgca cagacttaag ggttttctta tttctgaacg
    10801 ctgcactttt ttacctatct cttaaatagt cattttcaat acaacttttt ctttttcatc
    10861 acagccttaa tgctccttag ataataacca ttatctaaca atgagatacc taattcttag
    10921 cgtgcctgtt aacccacaca tcaggggcta tgcttattaa aagcaggcaa aaagcatgtt
    10981 ttacggtaaa gcccctgcgt ttgctgggtt gaactgataa tcattatcac taacatgctg
    11041 ttatacgctg aaagcgatga agtgaggtaa atagatggac atgcattcag gaacctttaa
    11101 cccacaggat ttcgcctggc aaggcttaac gctgacaccc gcagcggcga tacacatccg
    11161 cgagctggtg gcaaagcagc caggtatggt cggcgtgcgc ttaggtgtga agcaaacggg
    11221 ctgcgcgggc tttggctatg tgctcgacag tgttagcgag ccggacaaag acgatctgct
    11281 gtttgaacac gacggcgcga agctgtttgt cccgctgcaa tcgatgccgt ttattgatgg
    11341 cacggaaatc gatttcgttc gtgaaggact taatcagata ttcaaatttc acaaccctaa
    11401 agcccagaat gaatgtggct gtggcgaaag ctttagggta taggcggtac tatgtctcgt
    11461 aatactgaag caactgacga tgtcaaaacc tggaccggcg gcccgctgaa ttataaagaa
    11521 ggattcttca cccagttagc caccgatgag ctggcaaagg ggataaacga agaggtggtg
    11581 cgcgcaattt cggcgaagcg taatgagccg gagtggatgc tggagtttcg tctaaacgcc
    11641 tatcgcgcat ggctggagat ggaagaaccg cactggttga aagcgcacta cgacaagctg
    11701 aattatcagg attacagcta ctactcagca ccatcgtgcg gtaattgtga cgacacttgc
    11761 gcgtctgaac ctggcgcggt gcagcaaact ggcgcgaacg cctttttaag taaagaggtg
    11821 gaggcggcgt ttgagcagtt gggcgttccc gtgcgggaag gcaaagaggt ggcggtggat
    11881 gccattttcg actctgtttc ggttgccacc acttatcgcg aaaaactggc ggagcaggga
    11941 attattttct gttcctttgg tgaggcgatc cacgatcacc cggaactggt gcgtaaatat
    12001 ctcggcaccg tggtgccggg gaatgacaac ttctttgcag cacttaatgc ggcggtagcc
    12061 tctgacggta cgtttattta tgtgcctaaa ggcgtgcgct gcccgatgga actttccacc
    12121 tattttcgca ttaacgcgga aaaaaccggg cagtttgagc gcacaattct ggtggccgac
    12181 gaagacagct acgtcagcta cattgaaggc tgttccgctc cggtgcgtga cagctatcag
    12241 ttgcacgcgg cagtggtgga agtcatcatc cataaaaacg ccgaggtgaa atattccacg
    12301 gtacaaaact ggttccctgg cgataacaac accggcggta ttctcaactt cgtcaccaag
    12361 cgtgctttgt gcgaaggcga aaacagcaaa atgtcatgga cgcaatcaga aaccgggtca
    12421 gcgattacgt ggaaatatcc cagctgcatt ttgcgcggcg ataactccat tggtgagttt
    12481 tactcagtgg cgctgaccag cggtcatcag caagcggata ccggcaccaa gatgatccac
    12541 atcggtaaaa acaccaaatc gaccattatc tcgaaaggga tctctgccgg acatagtcag
    12601 aacagttatc gcggcttagt gaaaatcatg ccgacggcaa ccaatgcgcg caatttcact
    12661 cagtgcgact caatgctgat tggcgctaat tgtggggcgc ataccttccc gtatgtcgaa
    12721 tgtcgcaata acagcgcaca actggagcac gaggcaacga catcacgtat tggtgaagat
    12781 caactgtttt actgcctgca acgcgggatc agcgaagaag acgccatctc gatgattgtt
    12841 aacggtttct gcaaagacgt gttctcagaa ctgccgctgg aatttgccgt tgaagcacaa
    12901 aaactcctcg ccatcagtct tgaacacagc gtcggataag gaataaacat gttaagtatt
    12961 aaagatttac acgtcagcgt ggaagataaa gctatcctgc gtggattaag cctcgacgtt
    13021 cgtcccggcg aagttcacgc cattatgggg ccaaacggtt cgggcaaaag tactttatcg
    13081 gcaacgcttg ccgggcgcga agattatgaa gtgacgggcg gcacggttga gttcaaaggc
    13141 aaagatttgc ttgcactgtc gccggaagat cgtgcaggcg aaggcatctt tatggccttc
    13201 cagtatccgg tggagattcc aggcgtcagt aaccagtttt tcctgcaaac ggcacttaat
    13261 gcagtgcgca gctatcgcgg ccaggaaacg cacgaccgct ttgattttca ggatttgatg
    13321 gaagagaaaa tcgctctcct gaagatgccg gaagatttat taacccgttc ggtaaacgtt
    13381 ggtttttccg gcggcgagaa aaagcgcaac gatattttgc aaatggcggt gctggaaccg
    13441 gagttatgca ttcttgatga gtcggactcc gggctggata ttgacgcatt aaaagtggtc
    13501 gccgatggcg tgaactcgct gcgtgatggc aagcgctcat tcatcattgt tacgcactac
    13561 caacgcattc tcgactacat caagcctgat tacgttcatg tgctatatca gggacgaatt
    13621 gtgaaatccg gcgatttcac gttggtcaaa caactggagg agcagggtta tggctggctt
    13681 accgaacagc agtaatgcgc tgcaacagtg gcatcacttg tttgaagctg aagggacaaa
    13741 acgctcaccg caagcacagc agcatttaca acaattgctg cgtaccggac tgccgacacg
    13801 taaacatgaa aactggaaat atacgccgct ggaagggctg accaatagtc agtttgtcag
    13861 cattgcggga gatatatccc cacagcagcg tgatgcctta gcgttaacgt tagacgccgt
    13921 gcggctggtg tttgtcgatg gacgttacgt gcccgcgctg agcgatgcaa ttgaaggcag
    13981 cggatatgaa gtgagcatta acgacgaccg tcaggggtta cccgacgcta ttcaggcgga
    14041 agtgtttctg catttgacgg aaagcctggc acaaagcgtg acgcatatcg ccgtgaagcg
    14101 cggtcaacgg ccggcaaagc cattgctgtt aatgcatatc acccagggcg tggcaggtga
    14161 agaggtgaac actgcccatt accggcatca tctggatctg gcggaaggtg ccgaagcaac
    14221 ggtgatcgaa cattttgtca gccttaatga tgctcgtcat tttaccgggg cacggttcac
    14281 tatcaatgtc gcagcgaacg cccacttgca gcatatcaag ctggcgtttg aaaacccggt
    14341 cagtcaccac tttgcccata acgatttgtt gctggctgac gatgccaccg catttagcca
    14401 tagtttcctg ctgggtggcg cagtgttacg acacaacacc agtacgcaac tcaatggcga
    14461 aaacagcacg ctgcggatca atagcctggc gatgccggtg aaaaacgagg tgtgtgatac
    14521 ccgtacctgg ctggaacaca ataaaggttt ttgtaacagc cgacagttgc acaaaactat
    14581 cgtcagcgac aaaggccgcg cggtatttaa cggtttgatc aacgtcgcgc agcacgccat
    14641 caaaacggat ggtcagatga ccaacaataa tctgctgatg ggcaaactgg cggaagtgga
    14701 tacaaaaccg cagctggaaa tctatgcaga tgatgtgaaa tgcagccacg gcgcgacggt
    14761 ggggcgtatt gatgatgaac agatgttcta tctgcgctcg cgcgggatca atcagcagga
    14821 tgcccagcag atgatcattt acgcctttgc tgccgaactg acggaagcac tgcgtgatga
    14881 ggggcttaaa cagcaggtgc tggcccgaat cggtcaacgg ctgccaggag gtgcaagatg
    14941 actttttccg tcgacaaagt gcgggccgac tttccggtgc tttcgcgtga ggtaaacggt
    15001 ttgccgctgg cttatctcga cagcgccgcc agtgcacaga aaccgagcca ggtgattgac
    15061 gccgaggccg agttttatcg tcatggctac gcggcggtgc atcgcggtat tcatacctta
    15121 agcgcccagg cgaccgagaa aatggagaac gtgcgcaagc gggcatcgct gtttattaat
    15181 gcccgttcgg cggaagagct ggtgttcgtc cgcggcacga cggaagggat caatctggtc
    15241 gccaatagct ggggcaacag caatgtgcgg gcgggcgata acatcatcat cagtcagatg
    15301 gagcaccacg ctaacattgt cccctggcag atgctttgcg cacgcgttgg cgcagagctg
    15361 cgtgtgatcc ctctcaaccc cgacggtacg ctgcaactgg agacgctgcc tacgctgttt
    15421 gatgagaaaa ctcgcctgct ggcaattact catgtctcca acgtgcttgg cacagaaaat
    15481 ccactggcgg aaatgatcac gcttgcgcac cagcatggcg caaaagtgct ggtggatggc
    15541 gctcaggcgg tgatgcatca tccggtggat gttcaggcgc agggttgcga cttttacgtg
    15601 ttctccgggc ataaactgta tggccccacc ggaattggca ttctttatgt gaaagaagcc
    15661 ttgttgcagg agatgccgcc gtgggaaggg ggcggttcta tgatcgccac cgtcagcctg
    15721 agtgaaggca ctacctggac caaagcacca tggcggtttg aagccggtac acccaatacc
    15781 gggggcatca ttggtcttgg cgcggcgctg gagtatgttt cggcgctggg gcttaatagc
    15841 atagccgagt atgaacagaa tctgatgcat tatgcgctat cacagctgga atctgtaccg
    15901 gatctcactc tctatggcca acaaaacagg cttggcgtta ttgcttttaa tctcggtaaa
    15961 caccacgcct atgatgttgg cagttttctc gataattacg gcattgctgt gcgtaccgga
    16021 catcactgcg ctatgccatt aatggcctat tacaacgtcc ctgcgatgtg tcgggcgtcg
    16081 ctggccatgt ataacaccca tgaagaagtg gatcgtctgg tgaccggcct gcaacgtatt
    16141 caccgtctgc tgggataaca gggaggcact atggctttat tgccggataa agaaaaattg
    16201 ctgcgtaatt ttttacgctg cgccaactgg gaagagaaat atctctacat tattgagctg
    16261 ggccagcgtc tgccagaatt acgcgccgaa gacggaagcc cacaaaatag cattcagggc
    16321 tgccagagtc aggtgtggat tgtcatgcgc cagaatgcgc agggaattat tgaattgcag
    16381 ggcgacagcg atgcggcgat tgtgaaaggg cttattgcgg tcgtctttat tctctacgat
    16441 cagatgacgc cgcaggatat tgtcaatttc gatgtgcgtc cgtggtttga aaaaatggcg
    16501 ctcacccaac atctcacccc atctcgttca caaggtctgg aagcgatgat tcgcgcaatt
    16561 cgcgccaaag ccgctgcact tagctaaact aaagggacag ctttcatccg gttggctcac
    16621 attgccggat ggtcaatgtt tacactgacc tgcacaggag tgttttggtt tcaggtgaac
    16681 ataagcacca tccgacgttt cagcgtgagt ctccggcaaa tacaggaggt ttacaatgaa
    16741 acgcgcgtct ttgcttacac tcacgcttat cggcgctttt agcgccatcc aggctgcctg
    16801 ggcggttgat tatccgctac caccaaccgg aagccgactg gttgggcaaa atcaaacgta
    16861 tacggtgcaa gaaggggata aaaaccttca ggctattgcc cgacgttttg atactgcggc
    16921 aatgttgatc cttgaagcca ataacactat cgccccggtg ccaaaacctg gtacgacgat
    16981 aactattcct tcacaactgt tattacctga tgcaccgcgt caggggatta tcgttaacct
    17041 tgcagagctg cgcctttatt attatccgcc gggagaaaat attgtgcagg tttatccaat
    17101 aggtattgga ttgcaggggc tggaaacgcc ggtgatggaa acgcgtgttg ggcagaaaat
    17161 ccctaaccca acctggacgc ctacggcagg cattcgtcag cgttcgctgg agcgtggcat
    17221 taaattaccg ccagtcgttc ctgccggacc aaataacccg ctaggacgtt acgcactgcg
    17281 cctcgcgcat ggtaatggcg aatacctcat tcatggcacc agtgcgccgg acagcgttgg
    17341 tttgcgcgtc agttcagggt gtattcgcat gaatgcaccg gatattaaag ccttgttctc
    17401 cagcgtgcgg acgggaacgc cggtgaaagt gatcaacgaa ccggtgaaat attccgtgga
    17461 acctaacggg atgcgttatg ttgaagtaca tcgaccacta tcagcagaag aacagcaaaa
    17521 cgttcagaca atgccataca cactgccagc aggctttacg caatttaaag acaataaggc
    17581 tgtagatcag aagttagtcg ataaagcgtt gtatcgtcgg gcagggtatc cggtttcggt
    17641 gagcagtgga gcaactcccg cagccagcaa tgcgccttca gtagagtcag cgcagaatgg
    17701 tgaaccagag caagggaata tgttacgcgc gacgcagtag tggtaaatag cagacaaaaa
    17761 aaatggcgca caatgtgcgc catttttcac ttcacaggta ctattacttg cggtatttag
    17821 tagccatgtt gtccagacgc tggttagcac gagctgcgtc atctttagca gcctgaacgt
    17881 cggaacgcat tgcgttcacg tcgttgctca gctggtcaac tttagcgttc agagtctgaa
    17941 cgtcagaaga cagctgatcg attttagcgt tgctggagca acctgccagc agagtagaac
    18001 ccaggattac cgcgcccagt accagtttag tagctttcat tattaatacc ctctagattg
    18061 agttaatctc catgtagcgt tacaagtatt acacaaagtt ttttatgttg agaatatttt
    18121 tttgatggga aggcacttat ttttgatcgt tcgctcaaag aagcatcgaa cgctgggttt
    18181 tagataaaaa aattgagaga aaacaggatg cttccatcgg attcatctta aataaagtga
    18241 gattatatag tgaaatccga tttg
//
LOCUS       DADLTF010000077        17458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.077, whole genome shotgun sequence.
ACCESSION   DADLTF010000077 DADLTF010000000
VERSION     DADLTF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.077"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            564..1334
                     /locus_tag="JN749_002464"
     CDS             564..1334
                     /locus_tag="JN749_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HAY7368840.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="JN749_002465"
     CDS             1325..1798
                     /locus_tag="JN749_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAY7368841.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="JN749_002466"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="JN749_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HAY7368842.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPVSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="JN749_002467"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="JN749_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HAY7368843.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="JN749_002468"
     CDS             3233..3727
                     /locus_tag="JN749_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HAY7368844.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="JN749_002469"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="JN749_002469"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HAY7368845.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /locus_tag="JN749_002470"
     CDS             complement(6410..7432)
                     /locus_tag="JN749_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAY7368846.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8485
                     /locus_tag="JN749_002471"
     CDS             7571..8485
                     /locus_tag="JN749_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7368847.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGILTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8699..10060
                     /locus_tag="JN749_002472"
     CDS             8699..10060
                     /locus_tag="JN749_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368848.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JN749_002473"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="JN749_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HAY7368849.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JN749_002474"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="JN749_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HAY7368850.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /locus_tag="JN749_002475"
     CDS             12610..13899
                     /locus_tag="JN749_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HAY7368851.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPQVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="JN749_002476"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="JN749_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HAY7368852.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVDDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLHQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="JN749_002477"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="JN749_002477"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HAY7368853.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="JN749_002478"
     CDS             16225..16605
                     /locus_tag="JN749_002478"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HAY7368854.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17458
                     /gene="hpaH"
                     /locus_tag="JN749_002479"
     CDS             16673..>17458
                     /gene="hpaH"
                     /locus_tag="JN749_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HAY7368855.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGHPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSI"
BASE COUNT         4294 a         4627 c         4374 g         4163 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccggtaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttgccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcat
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacggttt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc
     8281 agtcatcttt cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat
     8341 tgccgtcgat gaacatatga ccattctcag cgctctgatt tgccgcagtg accttgatgc
     8401 cacccttgcg ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa
     8461 tgagaacacg cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc
     8521 acggttttat caaaagataa ataccggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agttcggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccaccagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg caggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtggacg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcac cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgagct gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcat ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccatt
//
LOCUS       DADLTF010000078        17414 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.078, whole genome shotgun sequence.
ACCESSION   DADLTF010000078 DADLTF010000000
VERSION     DADLTF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.078"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            132..575
                     /locus_tag="JN749_002480"
     CDS             132..575
                     /locus_tag="JN749_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7368856.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /locus_tag="JN749_002481"
                     /pseudo
     CDS             811..1630
                     /locus_tag="JN749_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JN749_002482"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="JN749_002482"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HAY7368857.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTCIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JN749_002483"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="JN749_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HAY7368858.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JN749_002484"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="JN749_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7368859.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /locus_tag="JN749_002485"
     CDS             complement(4321..4470)
                     /locus_tag="JN749_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HAY7368860.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="JN749_002486"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="JN749_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HAY7368861.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="JN749_002487"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="JN749_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HAY7368862.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="JN749_002488"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="JN749_002488"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAY7368863.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="JN749_002489"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="JN749_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HAY7368864.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="JN749_002490"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="JN749_002490"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAY7368865.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="JN749_002491"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="JN749_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HAY7368866.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="JN749_002492"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="JN749_002492"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAY7368867.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="JN749_002493"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="JN749_002493"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HAY7368868.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13936..14316
                     /locus_tag="JN749_002494"
     CDS             13936..14316
                     /locus_tag="JN749_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368869.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="JN749_002495"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="JN749_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HAY7368870.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /locus_tag="JN749_002496"
     CDS             complement(15368..16087)
                     /locus_tag="JN749_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HAY7368871.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="JN749_002497"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="JN749_002497"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HAY7368872.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
BASE COUNT         4443 a         4546 c         4323 g         4102 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 accacttgta atcaattgat tataaaggat taattcataa agagcattta cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgcag gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gatgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag atgacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggcgga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tataccaacc gcatcgccaa tagtcgttat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattacagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagtatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcgccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatga gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgtcgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctaga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctttcctaca atttctaact gtaattcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca cgccaccatt cagatggaat
    15301 atcaaccttg tcatgggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 accgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggcca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaattcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgtttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgcaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcgc attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttac
//
LOCUS       DADLTF010000079        17401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.079, whole genome shotgun sequence.
ACCESSION   DADLTF010000079 DADLTF010000000
VERSION     DADLTF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.079"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JN749_002498"
     CDS             complement(768..1964)
                     /gene="hemY"
                     /locus_tag="JN749_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HAY7368873.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1967..3178)
                     /gene="hemX"
                     /locus_tag="JN749_002499"
     CDS             complement(1967..3178)
                     /gene="hemX"
                     /locus_tag="JN749_002499"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HAY7368874.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3200..3940)
                     /gene="hemD"
                     /locus_tag="JN749_002500"
     CDS             complement(3200..3940)
                     /gene="hemD"
                     /locus_tag="JN749_002500"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HAY7368875.1"
                     /translation="MSILVTRPSPAGEELVRRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3937..4878)
                     /gene="hemC"
                     /locus_tag="JN749_002501"
     CDS             complement(3937..4878)
                     /gene="hemC"
                     /locus_tag="JN749_002501"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HAY7368876.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5265..7811
                     /gene="cyaA"
                     /locus_tag="JN749_002502"
     CDS             5265..7811
                     /gene="cyaA"
                     /locus_tag="JN749_002502"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HAY7368877.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7851..8171)
                     /gene="cyaY"
                     /locus_tag="JN749_002503"
     CDS             complement(7851..8171)
                     /gene="cyaY"
                     /locus_tag="JN749_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HAY7368878.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8219..9532)
                     /locus_tag="JN749_002504"
     CDS             complement(8219..9532)
                     /locus_tag="JN749_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HAY7368879.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9529..9834)
                     /locus_tag="JN749_002505"
     CDS             complement(9529..9834)
                     /locus_tag="JN749_002505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368880.1"
                     /translation="MFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFD
                     GAQLWRYRTADPKQVRLIWKEQHSGYVYGVNYKTGYLDGKITDDGEFIPAPRNEVTK"
     gene            complement(9856..11007)
                     /locus_tag="JN749_002506"
                     /pseudo
     CDS             complement(9856..11007)
                     /locus_tag="JN749_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005064826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11196..11399
                     /locus_tag="JN749_002507"
     CDS             11196..11399
                     /locus_tag="JN749_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAY7368881.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11436..12260
                     /gene="dapF"
                     /locus_tag="JN749_002508"
     CDS             11436..12260
                     /gene="dapF"
                     /locus_tag="JN749_002508"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAY7368882.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            12257..12964
                     /locus_tag="JN749_002509"
     CDS             12257..12964
                     /locus_tag="JN749_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HAY7368883.1"
                     /translation="MKQLGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12961..13857
                     /gene="xerC"
                     /locus_tag="JN749_002510"
     CDS             12961..13857
                     /gene="xerC"
                     /locus_tag="JN749_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAY7368884.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13857..14573
                     /gene="yigB"
                     /locus_tag="JN749_002511"
     CDS             13857..14573
                     /gene="yigB"
                     /locus_tag="JN749_002511"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HAY7368885.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14657..16819
                     /gene="uvrD"
                     /locus_tag="JN749_002512"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14657..16819
                     /gene="uvrD"
                     /locus_tag="JN749_002512"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HAY7368886.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16966..17358)
                     /locus_tag="JN749_002513"
     CDS             complement(16966..17358)
                     /locus_tag="JN749_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368887.1"
                     /translation="MRLGGVFYVAGDKVGIVRLDAFKASKDIQFAVQSGAMLMENGVI
                     NPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVEQLLYLGGT
                     ISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT         4059 a         4482 c         4606 g         4254 t
ORIGIN      
        1 gttgacgggg tatctgctgc gctggccatg agaggaagag cggcggcgac agcaacaaca
       61 agacgtttgg gtgaaaacga aaattccatg caaaatgctc cggtttcatg tcatcaaaat
      121 gatgacgtaa ttaagcattg ataattgaga tccctctccc tgacaggatg attacataaa
      181 taatagtgac aaaaataaat tatttattta tccagaaaat gaattggaaa atcaggagag
      241 cgttttcaat cctacctctg gcgcagttga tatgtaaggc aggtttatta tatcgcgttg
      301 attattgatg ctgtttttag ttttaacggc aattaatata tgtgttatta attgaatgaa
      361 ttttatcatt cataataagt atgtgtagga tcaagctcag gttaaatatt cactcaggaa
      421 gttattactc aggaagcaaa gaggattaca gaattatctc ataacaagtg ttaagggatg
      481 ttatttcccg attctctgtg gcataataaa cgagtagatg ctcattccat ctcttatgtt
      541 cgccttagtg cctcataaac tccggaatga cgcagagccg tttacggtgc ttatcgtcca
      601 ctgacagatg tcgcttatgc ctcatcagac accatggaca caacgttgag tgaagcaccc
      661 acttgttgtc atacagacct gttttaacgc ctgctccgta ataagagcag gcgttttttt
      721 atgcatcaga aaggtcccgg aggtgctttc ctccgggtga gaaggaacta ctgtggcggg
      781 ttattctgca acgttaacat caaaccgtcg cgacgcatcg ctgcagcttc ttccggcttg
      841 tgcagtctgt ccagcgcgtc ggcaagccat gcgtaatcgt aggcgtccgg acgttgtttc
      901 agcgccgcgc ggaaggcgag cgatgcttcc tgccattcac cgtgcttcat cagtgactga
      961 cccaatgtgc tccacaacag cgggcgatca ccgacgtttt tgatttgctg gcgtagcact
     1021 ttttccagct gttccgggtt attggttttc agacgcggga tcggcagcag taggcgatcg
     1081 tcatactggc gtttcaggcc gtcgatgata atttgctggg cagtatcatg atcgtcacat
     1141 tcaataagat gttccgccat tgccacctgc aacgctacct gatgacgcgt tttccggctt
     1201 tggtttttcc accagttacg caaaccttca ctaccgttat cggcacgcgc ctgatccatc
     1261 aggccaatcc atgcctgttg ttccagcatt gcacgatgtt cttcatcacc aacatgggct
     1321 ttcgccattg atgggatgat atccagcaga gaactccatg cccctgtgcg gatatacgcc
     1381 tgttccgcca gacgtaatac ttcaggatgg cgtggcgtaa cttccagcag cttatctacg
     1441 ccgtggcgtg cagcatggtt ttcattacgg gccagttgca gacgtacacg ggtgatttct
     1501 accggaatgg tgtcattgcc ggccagctcc gctgcgcgtt ccagatgttg gttggcgcgt
     1561 gcttcatcac cacgttgttg cgcggcttcg gcagccagta gatagttcac caccggttgt
     1621 tcagcgtgat cggcattttt cgccatcagc ttttcaactt gctgataatc accttccgcc
     1681 agtttcagca gcgcctgttc ggtctgctta cgcgcacggc gacgcttacg tccgacaaac
     1741 catccacggg tgtgcgcgcc agtgcggaag atccgccgca gtagccactc aatggcaaac
     1801 agcactacca tcgccagaat caatatgatc gccaggcccg tgacgctggt ttcgatattg
     1861 tagttgtcgg tctggatcag cacataaccc tgatgaccgg caatcatcgg gccaaccact
     1921 atccccgcaa tcagcaaaac aaagagtaat aacactttta gcatggttat tctccttgcg
     1981 gcgcggctgc cggagtatca ccttgcggtg caggtgcagg tgcaggtgca ggtgcaggtg
     2041 caggtgcagg tttagcttcc gttgtccccg ctgccggttg tgccagcagg ttacgcacgc
     2101 gagtctgcat cagtttttcc agcatcgcct ggctttgcag ggtttccgga agatccatcg
     2161 agatattttg ctggcttaac tggtccacct cgtcgaggaa cgctttggtg gtggcatcat
     2221 cagtatcgta gtaagcacgt acccaggtgg agacgttctc cagcgcctgg cgataagtct
     2281 cttcctgatg acgcggtaca gcttgtgctg cgaccagcag gcgagagcga atattttcgc
     2341 gcagatagat atcctgattt ggcgctaaca gcggtacggc ggtgtcatca cgacggcgaa
     2401 tcgtaatgaa gttgtccata aagttctgcc agcttttttg cagattgata cgccattcgc
     2461 tgatggaact ggaaagctct tcaccgtctg aatccatcgg cgaaccatcg ctgtcattat
     2521 cggccagacg caggttatct acctgatttg aaagctgatt aagcttaagg atgatgccgt
     2581 cataatccac ctgcgatact gcagaaaggc tggcgatatc atcggtaatt gcccgacgaa
     2641 cggtaatcag actcgggtca ttcatatccg ccaggctggc gtctgcactt ttcagcaacg
     2701 ctgcagaggt cgtgacgtcc tgatcgctcc acagcttccg tccggcgagt ttcaccagaa
     2761 aatcggcctg agccagcagc caggttttag catcgctgcc ggaaatggtg gcgatctttt
     2821 gttggacttc atccaactgt tttgccagcg tttcttgctg acgattcgcc tgcttaagct
     2881 gcgcagcttg ttgcttaata atgccttcca gctcggcttt ttggctctcc tgggcttttt
     2941 gcaatgccgt cagttggtta gccagggcat cgctggtggc ggtctgattg acggcctgtt
     3001 gtttacccca gccatacaaa ccgacacccg ccgccagagc aatagcgata gccaccgcgc
     3061 tgagaatcaa tgcagtattg ttcttactct ttttttctgt tgcgacaggt tgtgacgtgg
     3121 tgtccacggc ctccctggtc tcttcaacca cggcggaggt tttttcttgt tccgtcatta
     3181 tggcttcctg ttatgagagt tattgtaatg cccgtaaaag cgcatcgttg tcagcgttat
     3241 cggcgacctt aatgtcttgc cagcccagtt cccgggcgag tttcgccaaa cgctcactga
     3301 cgaccaatag tcgacagtgt agtaaccagt gctcacgata ccattgtggg atcagcgacc
     3361 agagttgctg caacatttca ccgctggtaa caacgaccat cgtcacctcg cgggattgcc
     3421 agcgcatcgc ttcttctgca ccatcgtaat ggattgcgca tcgttgataa cattcacaaa
     3481 aagtgacctc agcaccgcgc accgtcaggg tatccccaat tagctcacga ccgccattgc
     3541 cacgtaatat cagcgcacgt ttgcccgcaa tattttgtaa ttcaggtaat tgtagcaaga
     3601 cttcgctgat ttcccgatcc tgtgggtaga gaatcttctg tccgcttacg gtatgtagcg
     3661 ccagtgcggt ggtgcgtcca atggtgaaat aagtaggtag ttggggccat ttacgatctt
     3721 gctgatgcag ctgtgattgg gcaaaagcaa ccgcgtgttg cgagagggca aacaacagat
     3781 cgctctcccc cagcgctgcc agttgatcag caagttgcgg taattgtcga cccggagaaa
     3841 actcaatcag cggaaaatgc caggccacct gccccagtgt gcgcagacgt ctcactaact
     3901 cttctccagc gggagacggg cgggtgacca ggatactcat gccggggcct ctccgttata
     3961 gacttcagcg aggatctcgc gcgcgccgtt attcagtagc tcttctgcca gcgaaatccc
     4021 catttgttcg gcatcttgcg gcgcaccgcg gcgttcaccg cgaataatct gcgaaccgtc
     4081 cggcgcgccg accagcgcac atagccagat ttcgccatca ataagctcgg cgtagctacc
     4141 aattggcacc tgacatccgc cttcgagacg ggtattcatg gcgcgttctg cggtaacgcg
     4201 cagtgcagtt tcgtggtgat tcagcgcggc aagcagctcg cgagtgcgtg tatcatcaag
     4261 gcggcattca ataccgactg caccttgtcc taccgccgga agagaaatct cgggtggtaa
     4321 cgcggcgcga atgcgggact ccagacctaa acgttttagt ccggctacgg caagaatgat
     4381 ggcatcgtat tcgccgttat ctagtttgct caggcgagtg ccgacgttgc cgcgcaggga
     4441 gcggataatc agatccgggc ggcgttcagc cagttggcac tggcgacgta aactggacgt
     4501 cccgacgata ctgcctgctg gtaacgcatc cagactgtca tagttattgg acacaaaggc
     4561 atcgcgagga tcttcacgct cgcaaatagt gaccagcccc agaccttgcg ggaattcaac
     4621 cggcacatct ttcattgaat gtacggcgat atcagcgcga ttctccagga gcgcgacttc
     4681 cagctctttt acaaataagc cttttccgcc tactttcgcc agcggcgtat caagaatcac
     4741 atcgccgcgc gtcaccatcg gtaccagttc aacgaccagg cccggatggc tcgccatcaa
     4801 cttgtctttg acatagtgtg cctgccagag tgcaagtggg ctttggcgtg tggcaattct
     4861 taaaacattg tctaacatgc ttgttaccgt cattatcatc cgtggtccat cctaacatcc
     4921 ttgccagagt gatgtcagtg ttgtggtgaa acgtagacgc ctgcgcaaac cgtaaaatga
     4981 ggtctggcag tggatcctga caggcgtttc acgccgtcgt aataaggaat ttacagagaa
     5041 taaacggtgc tacacttgta tgtagcgcat ctttctttac ggtcaatcag caaggtgtta
     5101 aattgatcac gttttagacc attttttcgt cgtgaaacta aaaaaaccag gcgcgaaaag
     5161 tggtaacggt tacctttgac atacgaaata tcccgaatgc cgcgtgttac cgttgatgtt
     5221 ggcggaatca cagtcatgac gggtagcaaa tcaggcgata cgtcttgtac ctctatattg
     5281 agactctgaa acagagactg gatgccataa atcaattgcg tgtggatcgc gcgcttgctg
     5341 ctatggggcc tgcattccaa caggtctaca gtctactgcc gacattgttg cactatcacc
     5401 atccgctaat gccgggttac cttgatggta acgttcccaa aggcatttgc ctttacacgc
     5461 ctgatgaaac tcaacgccac tacctgaacg agcttgaact gtatcgtgga atgtcagtac
     5521 aggatccacc gaaaggtgag cttccaatta ctggtgtata caccatgggc agcacctcgt
     5581 ccgtagggca aagttgttcc tctgacctgg atatctgggt ctgtcatcaa tcctggctcg
     5641 atagcgaaga gcgccagtta ctacaacgta aatgcagcct gctggaaagc tgggccgcct
     5701 cgctgggtgt ggaagtcagc ttcttcctga ttgatgaaaa ccgcttccgt cataatgaaa
     5761 gcggcagcct ggggggcgaa gattgtggct ccacccagca tatactgctg cttgacgaat
     5821 tttatcgtac tgccgtgcgt ctcgccggta agcgtattct gtggaatatg gtgccgtgcg
     5881 acgaagaaga gcattacgac gactatgtga tgacgcttta cgcgcagggc gtgctgacgc
     5941 caaatgaatg gctggatctc ggtggcttaa gctcgctttc tgctgaagag tactttggtg
     6001 ccagcctttg gcagctctac aagagtatcg attccccata caaagcggtg ctgaaaacac
     6061 tgctgctgga agcctattcc tgggaatacc cgaacccacg tctgctggcg aaagatatca
     6121 aacagcgttt gcacgacggc gagattgtat cgtttggcct cgatccatac tgcatgatgc
     6181 tggagcgtgt tactgaatac ctgacggcga ttgaagattt cacccgtctg gatttagtac
     6241 gtcgctgctt ctatttaaaa gtgtgcgaaa aactcagccg tgaacgcgcc tgtgtaggct
     6301 ggcgtcgcgc agtgttgagc cagttagtga gcgagtgggg ttgggacgaa gctcgtctgg
     6361 caatgctcga taaccgtgct aactggaaga ttgatcaggt gcgtgaggcg cacaacgagc
     6421 tgctcgacgc gatgatgcag agctaccgta atctgatccg ctttgcgcgt cgcaataacc
     6481 ttagcgtctc cgccagtccg caggatatcg gcgtactgac gcgtaaactg tatgccgcgt
     6541 ttgaagcatt gccaggtaaa gtgacgctgg taaacccgca gatttcaccc gatctctcgg
     6601 aaccgaatct gacctttatt tatgtgccgc cgggccgggc taaccgttca ggttggtatc
     6661 tgtataaccg cgcgccaaat attgagtcga tcatcagcca tcagccgctg gaatataacc
     6721 gttacctgaa taaactggtg gcgtgggcat ggtttaacgg cctgctgacc tcgcgcaccc
     6781 gtttgtatat taaaggtaac ggcattgtcg atttgcctaa gttgcaggag atggtcgccg
     6841 acgtgtcgca ccatttcccg ctgcgcttac ctgcaccgac accgaaggcg ctctacagcc
     6901 cgtgtgagat tcgccatctg gcgattatcg ttaacctgga atatgacccg acagctgcgt
     6961 tccgcaatca ggtggtgcat tttgatttcc gtaagctgga tgtcttcagc tttggcgaga
     7021 atcaaaattg cctggtaggt agcgttgacc tgctgtaccg caactcgtgg aacgaagtgc
     7081 gtacgctgca cttcaacggc gagcaatcga tgatcgaagc cctgaaaact attctcggca
     7141 aaatgcatca ggacgccgca ccgccagata gcgtggaagt cttctgttat agccagcatc
     7201 tgcgcggctt aatccgtact cgcgtgcagc aactggtttc tgagtgtatt gaactgcgcc
     7261 tttccagcac ccgccaggaa accgggcgtt tcaaggcgct gcgcgtttct ggtcaaacct
     7321 ggggcttgtt cttcgaacgc ctgaatgtat cggtacagaa gctggagaac gccatcgagt
     7381 tttatggcgc tatttcgcat aacaaactgc acggcttgtc agtgcaggtt gaaaccaatc
     7441 acgtcaaatt accggcggtg gtggacggct ttgccagcga agggatcatc cagttctttt
     7501 tcgaagaaac gcaagacgag aatggcttta atatctacat tctcgacgaa agcaaccggg
     7561 ttgaggtgta tcaccactgc gaaggcagca aagaggagct ggtacgtgac gtcagtcgct
     7621 tctactcgtc atcgcacgac cgcttcacct acggctcaag cttcatcaac ttcaacctgc
     7681 cgcagttcta tcagattgtg aaggttgatg gtcgcgaaca ggtgattccg ttccgaacaa
     7741 aatcgattgg caacctgccg cccgccaatc aggatcacga tacgccgcta ttacagcagt
     7801 atttttcgtg atgagcgtgc cggaaagcga ggcttatccg gcatgcaatc ttagcggaaa
     7861 ctgactgttt cacccgcctg ttgcgtcgcc gcctgttcca gcaaatccca gaaggtttcg
     7921 ccgctgcgat cgcaaatcca ttcatcgcct ttcaggtcaa aatggtagcc gccctgtttg
     7981 gttgccagcc acacctggtg cagcggctcc tggcggttaa taatgatttt gctgccgttt
     8041 tcaaaggtaa tggtcagtac gccgccgttg atttcgcagt cgatatcgct gtcgccatcc
     8101 ctgtcgtcca gacgctcttc aatggtcagc cacagttgat cagccaggcg atgaaattca
     8161 ctgtcgttca ttgttgtacc ctgtttttaa gtgatggcgg cagtatagcg gcatggggtc
     8221 agggcttcaa agtttgcacc tctgctgctg cgttccggca tgattcatcc gtcaccggaa
     8281 taatgatgtc tctgttttga acttcacgta cgttctcctc tctgacacga gttttcgccg
     8341 ggcaggggga gggcgtaatc cttacctctg tatcggtcca tgtataaaca ttgcgatcgt
     8401 cataagcgaa gcgatcgttt ttagttagcg gaccgttaac tggaataatt tgcaagctct
     8461 ttgaccgtaa ttggcggata aagtaagcat gctgtttgcc gctattaaat gagcgatcaa
     8521 tgaccaagtc tggcgtatcc agcgggatta tctcaagctg gtcttcacca aatgtcgata
     8581 attttgttac atacagccga tctttatatt gcgcaatatt gccagtaatc agatggaatt
     8641 gttcaggttc aatacctggt aggtctatcc acttgctcca ctgacggtcg ggcgtgagct
     8701 gtttacgcca gcgaacgcga ctttccccta cttcaaaaga gacgtcatca tctacgggta
     8761 tcgctttgcc tgataattga ccaaagggat aacgatgcag acccttattg tcgcgaaaaa
     8821 ccagcagtga atgaggtatc cagcgaatta tctcaaatga gtctgcattc gccgtaaggt
     8881 gaacgccatt taaataaata ttctgcccac tgcggataag cagatcattg gaatatgaat
     8941 ttctgtttac actcataaga cgtggattaa taccccacca tcttttttct aatacagtaa
     9001 catcactgct gctcccctga cgtcgaccgg acaagtagag atttttacta tccaccagcg
     9061 tggtagagtt accttcgata gcttttagtg tcgccagatc aacacgtgac gcgccgctat
     9121 tactttcggt gcgctgtccg tcaaaataga ggctatattt atcgaccgcg aagcggccca
     9181 tagcctgaaa gctggcaata tcgacagtgg gtgttcccgg tggattgcta acgattttgc
     9241 cacgccagag aataacgcgg ccgtctgtca cccagtggta ttcctcattt tctatgctta
     9301 tttcatcgga aggtatataa cccgttttcc cagagatatt tttcacaagg cgcaaaccgg
     9361 ccctgctcat ctccggaaac tgggcaatga ttttcttatc cccccaataa cctttaccgt
     9421 aagacataac accgtgatca ttgtaatagt cgaaccctat ggcctgtaca gcaggcgtca
     9481 gaaatgtgtg tcccaacagg atgagtgcaa ctgtgattat ttgacccttc attttgtcac
     9541 ctcattgcgc ggggcaggaa taaattcgcc gtcatcagta atttttccgt ccagatatcc
     9601 cgttttgtaa tttacgccat agacatatcc cgaatgttgt tccttccaga ttaagcgaac
     9661 ttgtttcgga tcagcggtgc ggtatcgcca gagctgcgcg ccatcaaagc tgtaaatgta
     9721 gcggttatcg cgggcatacc atctgcgcaa aaagggatgg tcattaagat gggcttcgcg
     9781 ttcatccgga gaacggttat ccagtaagat cagcggtccg tcagattcaa acacgtgtaa
     9841 accggaatgg tatggctacc cttcggcacg cgtaagtaaa tagccgtggc gtttcccggc
     9901 gttgaaacgt ttgttgataa taagattggg atcatcaagt ttcacgatct ccagcttacg
     9961 gtcaccagac tctgtttcga tgatggtata aagcgagtcc tgatactggg cgacagcatc
    10021 actgatgggt tgaaactgct ccggatcgag tccgggtaat tcttcctgtt tacaagcagg
    10081 tcctttacgc cagaacactt tcttttccag cagagaaaaa tccagacagt gctttgttaa
    10141 atcttcatcc cacgccagat tttccttgtc gaggacttta cgctgcagcc cgtttttatc
    10201 acgccaggtc aagagtgagc caggcatcca gcgcacgacg gaaaaggtgt ccgggtcggc
    10261 atcaagctgt tcgccgttga gcaggatttt ggtccgtgta cgagccaggg tatcccaggg
    10321 gccaaatggt acggcaatgc aggggttaaa cgtggtggaa aactttcctc gctgatccca
    10381 ggacttctgc gccagcacac ggaaactctc ggggtcagcg aggcgatgcc cattgatata
    10441 caagaaatta gcgtcacgta atatcagccc cagtaagtca ggatcccagg gaggccggaa
    10501 ttccacctga tgcagcgttt tgatatccaa gctatttcca ccaccattgt catcggtgcg
    10561 cttgccttca aaatagagac tgtgtttatc tgcggcaaaa tcaccccatg ccttgaacga
    10621 ggcgacatcg acgggaggtg tgccgggagg gttttgcaca acttcacccg cgtagaggat
    10681 attgcgacca tcgctatgcc atgaataatg aggatactag gcctgtgcgc cctcatagag
    10741 gtagtcggtt atcaaatcag tcagaggcgg ttctttatcc cattcgtcta gtgttgtcgg
    10801 gtctattaat aaattgggta accgacgcaa actcttgtaa ttcacgtcag gtagagaaag
    10861 atttgtttct ttataagggc gcaggtacac cacgcctttt ccctgtttct ggtaaatatt
    10921 atgttcaggt gcggaaagcc gacatcccca tgccgacgat aaaaacaatg aacaagccaa
    10981 caaacaaacc acattgcgat agtgcataaa gccatcctgg cgcgaggtgt tgatcacgaa
    11041 actgccagca gaacataaat cctcacgagt aagcgttata ctcgcagcat ttcctcactt
    11101 ttcagacttc ataaagagtc gctaaacgct tgcttttacg ccttctcctg cgatgataga
    11161 aagcagaaag cgatgaactt tacaggcaat ccataatgaa aaacgtgttt aaggcactca
    11221 ctgtattact tactctcttc agcctgacgg gctgcggtct gaaaggtccg ctctatttcc
    11281 cgcctgcaga taaaaacgca ccgccgccga ccaaaccggt agagacgcaa acgcaatcca
    11341 cggtgccgga taaaaacgat cgcgccactg gcgatggtcc atcccaggtg aattactaaa
    11401 agtcagtttc tgtacccgcg tgattggagt aaatgatgca gttctcgaaa atgcatggcc
    11461 ttggcaacga ttttatggtc gtcgacgcgg taacgcagaa tgtctttttt tcaccggagc
    11521 tgattcgtcg cctggcggat cggcacctgg gggtagggtt tgaccagctg ctggtggttg
    11581 agccgccgta tgatcctgaa ctggattttc actatcgcat tttcaatgct gatggcagtg
    11641 aagtggcgca gtgcggtaac ggtgcgcgct gctttgcccg ttttgtgcgt ctgaaaggac
    11701 tgaccaataa gcgtgatatc cgcgtcagca ccgccaacgg gcggatggtt ctgaccgtca
    11761 ccgatgatga tctggtccgc gtaaatatgg gcgaacccaa cttcgaacct tccgccgtgc
    11821 cgtttcgcgc taacaaagcg gaaaagacct atattatgcg cgccgccgag cagacaatct
    11881 tatgcggcgt ggtgtcgatg ggaaatccgc attgcgtgat tcaggtcgat gatgtcgata
    11941 ccgcggcggt agaaacgctt ggtcctgttc tggaaagtca cgagcgtttt ccggagcgcg
    12001 ccaatatcgg ttttatgcaa gtggttaagc gcgagcatat tcgtttacgc gtttatgagc
    12061 gtggggcagg agaaacccag gcctgcggca gcggcgcgtg tgcggcggtg gcggtaggga
    12121 ttcagcaagg tttgctggac gaagaagtac gcgtggaact ccccggcggt cgtcttgata
    12181 tcgcctggaa aggtccgggt cacccgttat atatgactgg cccggcggta catgtctacg
    12241 acggatttat tcatctatga agcaactagg ggaagaactg caggaaacac tcacggagct
    12301 tgatgaccgg gcggttgtcg attatctgat taaaaatcct gagtttttta tccgcaatgc
    12361 gcgcgcagta gaagcgatac gtgtgccgca tccggtacgc ggcaccgttt cgttggtcga
    12421 gtggcacatg gcccgcgcac gtaatcacat tcatgttctg gaagagaaca tggcgttgtt
    12481 gatggagcag gctatcgcca acgaagggct gttttatcgc ctactctacc tgcagcgcag
    12541 tctcaccgcc gccagcagtc tcgacgatat gctgatgcgc tttcaccgct gggcgcgcga
    12601 tctcggcctg gctggtgcga gtctgcgcct gtttccagat cgctggcgct taggtgcgcc
    12661 gtcgaaccac actcatctgg cattaagccg tcagtctttc gaaccgctgc gtattcagcg
    12721 tttggggcag gaacagcact atcttgggcc gcttaacgga ccggagctgc tggtggtgct
    12781 accggaagcg aaagcggtgg gatcggtggc gatgtcgatg ctgggaagcg atgctgattt
    12841 gggtgttgtg ctgtttacca gtcgcgatgc cagtcactat caacaagggc agggaacgca
    12901 gttacttcat gaaattgcgc tgatgttgcc ggagcttctg gagcgttgga ttgaacgcgt
    12961 atgaccgatt tacacaccga tgtagaacgc tacctacgtt atctgagcgt ggagcgtcag
    13021 cttagcccga taaccctact taactaccag cgtcagcttg aggcgatcat caattttgcc
    13081 agcgaaaacg gcctgcaaag ctggcaacaa tgcgatgcag cgatggtacg caattttgct
    13141 gtacgcagtc gccgtaaagg gctgggagca gcaagtctgg cgttacggct ttctgcgcta
    13201 cgtagctttt ttgactggct ggtcagccag aacgaactca aagctaaccc ggcgaaagga
    13261 gtttcggcac cgaaagcgcc gcgtcatctg ccgaaaaata tcgacgtcga cgatatgaat
    13321 cggctgctgg atattgatat caacgatccc ctcgctgtac gcgaccgtgc aatgctggaa
    13381 gtgatgtacg gcgcgggtct gcgtctttcc gagctggtgg ggctggatat caaacacctc
    13441 gacctggagt ccggcgaagt gtgggtgatg gggaaaggca gcaaagagcg ccgcctgccg
    13501 attggtcgca acgctgtggc gtggattgag cactggcttg atttgcgcga cctgtttggt
    13561 agcgaagacg acgcgctttt tctgtcgaaa ctgggcaagc gtatctccgc gcgtaatgtg
    13621 cagaaacgct ttgccgaatg gggcataaaa caagggctga ataatcacgt tcacccacat
    13681 aaattacgtc actcgttcgc tacgcatatg ctggagtcga gcggcgatct tcgtggtgtg
    13741 caggagctac tgggtcatgc caacctctcc accacgcaaa tctatactca tcttgatttt
    13801 caacaccttg cctcggtgta cgatgcggcg catccacgcg ccaaacgggg gaaataatgc
    13861 gtttttaccg gcctttgggg cgcatctcgg cgctcacctt tgacctggat gatacccttt
    13921 acgataaccg tccggtgatt ttgcgcaccg agcgagaggc gcttaccttt gtgcaaaatt
    13981 atcatccggc gctgcgcagc ttccagaatg aagatctgca acgcctgcgc caggcggtac
    14041 gggaagcgga acccgagatt tatcacgacg tgacgcgttg gcgttttcgt tcgattgaac
    14101 aagcgatgct cgacgccggg ctgagtgccg aagaagccag tgcaggcgca cacgcagcaa
    14161 tgattaactt tgccaaatgg cgcagccgaa tcgacgtccc gcagcaaact cacgacacct
    14221 taaaacagct ggcgaagaaa tggccgctgg tggcgatcac caacggtaac gcccagccgg
    14281 aactgtttgg tttgggggat tattttgagt ttgtgctgcg cgctggcccg cacgggcgct
    14341 caaaaccgtt cagcgatatg tactttttgg ctgcggaaaa actcaacgtg ccgatcggcg
    14401 agatcttaca tgttggggac gatctcacca ctgacgtggg tggggcaatt cgcagcggaa
    14461 tgcaggcttg ttggatcaga ccggaaaatg gcgatctgat gcaaacctgg gacagccgtt
    14521 tactgccaca tctggaaatt tcccggttgg catctctgac ctcgctgata taatcagcaa
    14581 atctgtatat atacccagct ttttggcgga gggcgttgcg cttctccgcc caacctattt
    14641 ttacgcggcg gtgccaatgg acgtttctta cctgctcgac agccttaatg acaaacaacg
    14701 cgaagcggtg gccgcgccac gcagcaacct tctggtgctg gcgggcgcgg gcagtggtaa
    14761 gacgcgcgta ctggtgcatc gtatcgcctg gctgatgagc gtggaaaact gctcgccata
    14821 ctcgattatg gctgtgacgt ttaccaacaa agcggcggcg gagatgcgtc atcgtatcgg
    14881 acaactgatg ggcaccagcc agggcggcat gtgggtaggc accttccacg ggctggcgca
    14941 ccgcctgctg cgtgcgcacc atatggacgc caatctgccg caggatttcc agatcctcga
    15001 tagcgaagac cagctacgcc tgcttaagcg tctgatcaaa gccatgaacc tcgacgagaa
    15061 gcagtggccg ccgcgccagg caatgtggta catcaacagc cagaaagatg aaggcctgcg
    15121 tccgcatcat attcaaagct acggtaatcc ggtggagcag acctggcaga aggtgtatca
    15181 ggcgtatcag gaagcgtgcg atcgcgcggg cctggtggac ttcgccgagc tgctgctgcg
    15241 cgctcacgaa ctgtggctta acaagccgca tatcctacaa cactaccgcg aacgttttac
    15301 caatatcctg gtggacgaat tccaggatac caacaacatt cagtacgcgt ggatccgcct
    15361 gctggcgggc gacaccggca aagtgatgat cgtcggtgat gacgaccagt caatctacgg
    15421 ctggcgcggg gcgcaggtgg agaatattca gcgtttcctt aatgatttcc ccggtgccga
    15481 aattattcgt ctggagcaaa actaccgctc taccagcaat attctgagcg ccgctaacgc
    15541 cctgattgaa aacaataacg ggcgtctggg taaaaaactg tggaccgatg gcgcggacgg
    15601 tgagcctatt tccctctatt gcgcttttaa cgaactcgat gaagcgcgtt ttgtggttaa
    15661 ccgcatcaaa acctggcagg acaacggcgg ggcgcttgcc gagtgcgcca ttctctaccg
    15721 cagtaacgcc cagtcgcgtg tgctggaaga agcactgttg caggcgagta tgccgtaccg
    15781 tatttacggc gggatgcgct tcttcgaacg ccaggaaatc aaagatgcgc tctcgtatct
    15841 gcgcctgatt gccaaccgta acgacgacgc ggcctttgag cgtgtggtga atacgccaac
    15901 gcggggtatt ggtgaccgga cgctggacgt ggtacgtcag acatcgcgcg atcgccagtt
    15961 aacactctgg caggcatgta gtgaactgtt gcaggaaaaa gccctcgccg gacgtgctgc
    16021 cagcgcctta cagcggttta tggaactgat cgacgcctta gcgcaggaaa ctgccgatat
    16081 gccgctgcat gtacagactg accgggtaat taaagactcc ggcctgcgta ccatgtacga
    16141 gcaggagaag ggcgaaaaag gtcagacgcg tatcgaaaac ttagaggaac tggtgacggc
    16201 aacgcgccag ttcagctaca acgaagaaga cgaagattta atgccgctgc aggcgttcct
    16261 atcccatgcg gcgctggaag caggcgaagg gcaggcggat acctggcagg atgcggtaca
    16321 actgatgacc atgcactcgg cgaaaggtct ggagttcccg caggtgttta tcgtcggtat
    16381 ggaagagggc atgttcccaa gccagatgtc gctggatgaa ggcgggcgtc tggaagaaga
    16441 acgccgtctg gcctacgttg gcgtaacccg cgcgatgcag aaactgacgc tgacctacgc
    16501 ggaaactcgc cgtctgtatg gcaaagaggt ttaccatcgc ccgtcgcgct ttattggcga
    16561 gttgccggaa gagtgtgtgg aagaggtgcg cctgcgcgcc acggtaagcc gcccggtcag
    16621 ccatcagcgg atgggtacgc cgatggtcga gaacgacagc ggctacaagc tcggccagcg
    16681 tgtacgccac gctaagtttg gtgaaggcac catcgtcaat atggaaggca gcggtgaaca
    16741 tagccgtttg caggtggcat tccagggcca gggaatcaaa tggctggtgg cagcttacgc
    16801 ccggctggag tcggtgtaac gttgccggat gcggtgctgc gcaccttatt tggcctaaaa
    16861 aatcattcag attcaataaa ttgcaacgtc atgtaggcag gataaggcgt ttacgccgca
    16921 tccggcatca gcagcatatt tagtatctga cacaaagcta tcattctaac ctttccgctc
    16981 gacggaaatc atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata
    17041 catatgtgaa atcgtgccac caagataaag cagctgctca acgttcagtt tcgctttggc
    17101 ataacaggca aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc
    17161 atgtttatta atcccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg
    17221 cggattaatt acaccgtttt ccatcaacat tgctcctgac tgcaccgcaa actgaatatc
    17281 tttactggct ttaaaagcat ccagacgaac gatgccgact ttatctcccg cgacataaaa
    17341 cacgccgcca agacggataa agaaattccc ttcacctgaa gcgagattta acgctacctt
    17401 c
//
LOCUS       DADLTF010000080        17105 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.080, whole genome shotgun sequence.
ACCESSION   DADLTF010000080 DADLTF010000000
VERSION     DADLTF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.080"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..172
                     /locus_tag="JN749_002514"
     CDS             <1..172
                     /locus_tag="JN749_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7368888.1"
                     /translation="CSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGA
                     VFGGELTLVQLQ"
     gene            complement(173..448)
                     /locus_tag="JN749_002515"
     CDS             complement(173..448)
                     /locus_tag="JN749_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HAY7368889.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDVYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(469..705)
                     /locus_tag="JN749_002516"
     CDS             complement(469..705)
                     /locus_tag="JN749_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HAY7368890.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDARIMRLA"
     gene            complement(823..2262)
                     /gene="rsmF"
                     /locus_tag="JN749_002517"
     CDS             complement(823..2262)
                     /gene="rsmF"
                     /locus_tag="JN749_002517"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HAY7368891.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2342..4840)
                     /gene="yebT"
                     /locus_tag="JN749_002518"
     CDS             complement(2342..4840)
                     /gene="yebT"
                     /locus_tag="JN749_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HAY7368892.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTITIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLMTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKV
                     TGEMTVDPSVVTLLRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVV
                     VPGEKALLQEPDVLTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAI
                     EPQHRELVKGDSKFVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASY
                     PLYANLEKALENSLSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAF
                     DIDLHIKPEYRNLLTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGA
                     SASQRKGDKRILYASETAARAVGRQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLI
                     TARNEVQAKAVLYPEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGN
                     PRRDFELEEATITDSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLS
                     DRVMIAMRISKRYQHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATP
                     PGTPLAPKAQEGKHFLLQESEPKEWREWGTALPK"
     gene            complement(4809..6093)
                     /gene="yebS"
                     /locus_tag="JN749_002519"
                     /pseudo
     CDS             complement(4809..6093)
                     /gene="yebS"
                     /locus_tag="JN749_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6223..6720
                     /locus_tag="JN749_002520"
     CDS             6223..6720
                     /locus_tag="JN749_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAY7368893.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6817..7515
                     /gene="proQ"
                     /locus_tag="JN749_002521"
     CDS             6817..7515
                     /gene="proQ"
                     /locus_tag="JN749_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HAY7368894.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7535..9583
                     /gene="prc"
                     /locus_tag="JN749_002522"
     CDS             7535..9583
                     /gene="prc"
                     /locus_tag="JN749_002522"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HAY7368895.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9775..10656
                     /gene="htpX"
                     /locus_tag="JN749_002523"
     CDS             9775..10656
                     /gene="htpX"
                     /locus_tag="JN749_002523"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HAY7368896.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10702..12075)
                     /locus_tag="JN749_002524"
     CDS             complement(10702..12075)
                     /locus_tag="JN749_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368897.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12252..13043
                     /gene="kdgR"
                     /locus_tag="JN749_002525"
     CDS             12252..13043
                     /gene="kdgR"
                     /locus_tag="JN749_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HAY7368898.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13186..13425)
                     /locus_tag="JN749_002526"
     CDS             complement(13186..13425)
                     /locus_tag="JN749_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368899.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13584..13727
                     /gene="mgrB"
                     /locus_tag="JN749_002527"
     CDS             13584..13727
                     /gene="mgrB"
                     /locus_tag="JN749_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HAY7368900.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13802..14089
                     /locus_tag="JN749_002528"
     CDS             13802..14089
                     /locus_tag="JN749_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368901.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14759..14902
                     /locus_tag="JN749_002529"
     CDS             14759..14902
                     /locus_tag="JN749_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HAY7368902.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14915..15124
                     /gene="cspE"
                     /locus_tag="JN749_002530"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14915..15124
                     /gene="cspE"
                     /locus_tag="JN749_002530"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HAY7368903.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15290..16099
                     /gene="rlmA"
                     /locus_tag="JN749_002531"
     CDS             15290..16099
                     /gene="rlmA"
                     /locus_tag="JN749_002531"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HAY7368904.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16096..16662)
                     /gene="mntP"
                     /locus_tag="JN749_002532"
     CDS             complement(16096..16662)
                     /gene="mntP"
                     /locus_tag="JN749_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HAY7368905.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4522 a         4134 c         4315 g         4134 t
ORIGIN      
        1 gtgtagccgc tcgcgattag gtgaacgccc aaaagggcag ggaattacag gtgctgatca
       61 tttctggttt ggtcatacac cgttgcgaca tcgcgtggat attggcaacc tgcattatat
      121 tgataccggt gctgtctttg ggggcgaact gactcttgtg caactgcaat aattaaaaat
      181 caccatactc ctgtgctggt cgccagaaac catctataaa atcctcaatc ggaaaacaac
      241 cgccatggcg gatccgttga tcgctcatag aataaagaca ctgctgttcc gtgttgtaga
      301 catccacaac aatatcttca caaccgccat ccaggtagca aacaaaaagt accagcgcga
      361 acatttcatc cccgaagtgt ggtgccgtac cgttaagttt aggagagatt ttacaacggg
      421 ggaataacca ggacaaataa cccgccatgc cgacgggttc tttttggatc aggcaagacg
      481 cataatcctg gcatcagatt gttgatcgta acgggtacgg taaaggacag agtgttcgcc
      541 atttagcagg gcagggatac tggtttcagc atcccaggca tccagttgca tacataaatc
      601 ggggtcagat ttacaaggaa tggttaacgt tcgatcaccc ggcgattcac cgtgtaattc
      661 gccgtcgtcg atttcaaaag cgcctatgcg cacactggtt ttcatcttcg tgctctccat
      721 ttagctgctg attgtggtac gaccagtccg atcgcacaat ttttcagcct agttgaggcc
      781 gccgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcatgcgt tactggtaaa
      841 aagtttcccg tcccgcacca gttcacgcgg gtagctgttt ttcagtcgcg aaccaatccg
      901 tttggctaaa ccaatcggct gatgctggaa agtaaccaac acgtcatccg ccactggcgc
      961 ggcttgcggg taaacatcgc gcccgcgata ccactcctcc gcttcctgcg gtgtcagttc
     1021 aaaagcgttt acattgtcgg gggaggcatg ggcaataacc gcttcatgct gccagcgata
     1081 acctttgtta tgcgtttcgg caagtttaat ccctaagcga gaaaatcgga ctttaccgat
     1141 cagggcttca atgcctaccg ggaacaacca cagttctttg tcgcgttgcc agagtcgcag
     1201 gtttccatcc cagtttaagc caacacttgc agccgcctga cgaatttgtc cggcttcgcg
     1261 atctttcacc gggctgaacg ggaaattgcc cactttgtat tttggggcgg gtaaggcggg
     1321 gatcgcctga gttttacgca gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg
     1381 gaaaacatgc aaaaaacctt cttcggtcag cgccttgttt gcaccaggga agagatcgcc
     1441 aagtggtaaa aactctactt cgtcggggta agtctctttc agccaccggc aaacggcttc
     1501 gttttcttcc cggtttaagg tacaggtcga gtaaaccagc gtaccgccag gacgtaatgc
     1561 atgaaaggcg ctgtcaatca gttcccgttg ggtagctgcg atttcctgat tgctttcggg
     1621 tgaccagttt tttagcgcat cgggatcttt acgcaccacg ccttcgccgg agcagggagc
     1681 gtccagcaaa atggcatcaa acatttctgg cactgacgca ccaaacacgc ggccaccaaa
     1741 atgtgtgagc gcaacattac tgatgccaca gcggctgata ttggcatgta acacttttac
     1801 ccgactggcg gaaaactcat tggcaaggat tgccccttcg ttattcatcc gcgcggcaat
     1861 ttgcgtcgtt tttgagcctg gcgcagcagc gacatccatc acccgctgtg gtgcattacc
     1921 gtcagcaaac aaggcggcga cgggcaacat tgaactggct tcctgaatat aaaacaggcc
     1981 acttaaatgc tcggtggtac tacccaatgg caatgcatct tcattgtcgc gttcaatcca
     2041 gaaaccttct tcacaccacg gaattggcgt aagcgtccag ccataaggag cggttaattg
     2101 caggaaatca gcaacggaga ttttcagcgt attaacgcga atgctgcggc gcaacgggcg
     2161 ctgacaggcg gcaagaaaat catcaaatga gagcgtcgaa ggcatagctt cgcgcatttg
     2221 tgtcagaaac gcgtccggga aataaacggc gtgttgggcc acgagcatgt accactggca
     2281 aaaaaacagg cgcgcagttt agcataaacg ctccggcgca ggcacgccgg agcagtgggc
     2341 attatttggg aagcgcagtt ccccattcac gccactcttt cggttcactt tcctgcaaca
     2401 ggaagtgttt gccttcctgg gctttcggtg caagtggcgt acccggaggc gtggcgaagg
     2461 cgatgccgcc acggataaat tggttaaagg tgccggtttt cactacgccg cccgtcagac
     2521 caaagtccag actgtaaccc gatgccaacc agaagacgga attgttacgc accaggtgtt
     2581 gatagcgttt actgatgcgc atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca
     2641 ttccggtaac cgtacctact tccagaccac ggaacagcac aggcgtaccg atgcctaacg
     2701 aaccggcttc cggcgcttca acaataatgc ttaaaccatc taggtaacgc gaatcagtaa
     2761 tggtggcctc ttctaattca aagtcgcgac gaggattgcc acggcctggt tcgacgttga
     2821 tatacggctg gaggatagta tctagatgct caacgccagc tgccgagatt tgcggtgtga
     2881 ccactgagaa gcgcgtacca ccgcgagcaa aggtctggac atattccgga tagagcaccg
     2941 cctttgcctg tacttcattg cgtgcggtaa tcagatccag cgtctggatt tgcccgatat
     3001 caataccgag atagcgaatt ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa
     3061 gcgtaatctg cctgccaacc gcacgggccg ctgtttcgga agcatacaga attcgcttgt
     3121 cgcctttacg ctgactggcg ctggcaccac tgaggttatc gaagctaatg gcccccttta
     3181 atgctctgga gagcggggat gcctgtacgg tcaggccact accattcagc tgaactttcg
     3241 ccccgccttc tgcccagaac acgctgttgc tggtcaaaag gttgcgatat tccggtttaa
     3301 tgtgcagatc gatatcgaac gcgttagctc gcggacgcac ggtaataact tcaccaactt
     3361 caaatttacg gtacagcact accgatcctg cctgcacatc cggcagcgtc tctgcactca
     3421 aactcacggt tgtggtgggt aaatcgctaa ggctgttctc cagcgctttt tccagattgg
     3481 catacagtgg atagctggct ttcatctcgc ctttatcgcc cggcagaata cgtatcccgc
     3541 cgttaatcca ttctgaggcg ctggcaccga gaaactcaac gccatccagc cccaccttca
     3601 cgtcgacacg gctgttgacg acaaatttgc tatcgccttt taccagttct cgatgctgag
     3661 gctcgatggc gacggtaaag gtgacgcctt tgctggtgag tttacgatcg ataacctggc
     3721 ctacctgcac gccgtgaaga atgagcggct gacccgcatc aataccgtaa ctttccggtg
     3781 ctgtcagggt cagtgtcaga acgtccggct cttgcagcag ggctttttcg cccggcacaa
     3841 caacgaactc tttgcgtggc tcgccatcgc cgggcaccaa ctcaaaggtt tttccggtca
     3901 gcagggcgct gagattagca tcgctaaggg ataatttcgg gttgcgtaat tctatgcggg
     3961 tattttcacg aagcagggta acgacgctgg gatcaacggt catttcccca gtgactttac
     4021 caccaggatt taaatccagt ttagtcagct gtccgacttc cagcccctga tacattaacg
     4081 gcgtcgagtc ggcggttaat cctgctccac tcggcagttc cagtttcacc ttcgcgccac
     4141 tgatactgac gttggcatca acgccggaaa cgttccagaa acggctacct tttttcacca
     4201 gatcggtaaa acgccgctcg atcaggacat caatcaccac gccttgcttg ttgggattga
     4261 tggcatagtc gtagactttt cccaccggga tcttgcggaa atagaccaat gaaccgctgt
     4321 tcagcgaacc gagatcgggg gcttgcaggt ggatcatcag atcgccattg tccagccgat
     4381 atttcggttg ggtatcgagc gcgacaaagt gatcctgctc tttaccttta cccggcatca
     4441 tgccgatata gttcccaccg acgagggcgt ccagcccgga gacacctgcc aacgatgctt
     4501 ttggcgtcat cagccagaac tgagtctctt cgcgcagcgc atctttcata tcggacttga
     4561 tgctgacctt gacttcaatc ttacgaagat cgtcgctgag gctgatatcc tgcactgttc
     4621 cgacttcaac gccctgataa cgaacaggcg tacggcccgg aacaatacca tccgccgaca
     4681 taaagtcgat ggtgatggta ttaccccggt cctgataact gtcccaaatc agccaaccgg
     4741 caatcattag cgcgatgaaa ggcagcagcc agaaaggtga gatacggcgt ttatttttaa
     4801 tctgcgcttc agtcgtcgaa gcgggcgttt cctgactcat gtgcatccca aagtaagcgg
     4861 ctgtccagcc attccacagc aagaatagtc aaaattacgg cagcgccaaa ataaaacgca
     4921 gccggtccca tagtaaaagc gaggatctga tcgcgattaa tcagcgacat ggttaaagat
     4981 atgacaaaca ggtcgagcat cgaccagcgt ccaatccagg tcaccatccg cagtaacaga
     5041 atgcgtgtgc gtaaaccttg ctggcattta aaatgaatgc tcaacagtaa agtgaacatg
     5101 acgatcactt tagtaaacgg taccagaata ctggcgataa acacgattcc cgcaaccgca
     5161 atgttgctac ttgccagcga cataattccg gaaagaatcg tatcttcctg ccggccacca
     5221 ttcagataaa tgatagaaat aggcaatagg ttggctggta acaacaaaac gattgacgct
     5281 aacagcgccg cccagcattt ttgcaaacta tgacgacgac gcaggcgtag cgggatatgg
     5341 caacgcgggc agagaccacg ctgatcagga tagccggtaa aatggcaccc aagacagaca
     5401 cgaagtttct cgtccctacg cgtagcgggg cgctgcggat aatatcgctc ccacagctcc
     5461 tcaacattaa gatgtgacaa cgtcaccgtc gttaaaatca ccaacgccac aaaagagaac
     5521 aagccgacac ccgcctggat atgggcataa tcctgtacct tatatagaag caacgccaat
     5581 gccgaccagg tagatatcca gcattaccca ctctttaagt cgctcaagca tcagcagtac
     5641 cggacgtaaa ttcattccca gtcggttacc aaaccataaa tatgctatgg aggacaccag
     5701 aatgaggggg gcaccaataa cgcagaaaaa gaccattgat cccgttatcg catcgccctg
     5761 tttggtcatt tgccagatgc cttgcatgac gttggcgtca atacgaatgc ctaacagcca
     5821 gatatgcaac agcggttcgc cccaggcaaa cggcataagc aacagcatgg tgaaggccat
     5881 tgccgccagg cgcgttagcg accagtcgcg cccgtcgcga atttttgcct gacagcgcgg
     5941 acaataggca ctttgatgag aatttatctc gggcaggcta aacagcatgt cacattgcgg
     6001 gcaacgttgg taatcaccac gcggcagttc ctcgccgatt gccctcactg ttatcttttt
     6061 tgtcggcgta atttgtggtg tgttaagagc catgacgtgc caaatgtaat aagaattaac
     6121 attaatctta acccatgatg ggaatgatct tgagcattaa cgcatttaat gcttatttta
     6181 ataggctggc agttccaggt aagacaacca agtgattaaa taatgaacaa aacagaattt
     6241 tacgcggatt taaatcgcga ctttaacgcg ctgatggcgg gagaaaccag ttttctggca
     6301 acgcttgcga acaccagtgc gttgttatac gagcgtctca ctgacgttaa ctgggcaggt
     6361 ttttatttgc ttgaggacga tacactggta ctcggaccat ttcagggcaa aattgcctgt
     6421 gtccggatac ccgtcgggcg cggggtgtgc ggcacagcgg ttgcccgcaa tcaagtgcag
     6481 cgtatcgagg atgttcacgc gtttgacggg catattgcct gtgatgcggc gagtaattct
     6541 gaaattgttc tgccgctggt ggtgaaaaat cagattattg gtgttctcga catcgatagt
     6601 accgtcttcg ctcgctttac agacgaggac gagcaaggct tacgtcagct tgtggcacag
     6661 cttgaaaaag tgcttgcaac gacggattac aaaaaattct ttgcgagcgt cgcaggataa
     6721 tcaacggata acgtagcatt tactggtagc gtcattataa tgacgcctgt tcatgcctgc
     6781 gcttgttggc tacgtccgtt gtaatcagga aatttcatgg aaaatcaacc taagttgaat
     6841 agcagtaaag aagtaatcgc gtttctcgcc gaacgttttc cccactgttt cagtgcggaa
     6901 ggtgaagcgc gtccgctgaa aattggtatt tttcaggatt tggtcgatcg tgttgccggg
     6961 gaaatgaacc tgagcaaaac gcaattacga tccgctttac gtctctacac ttcgagctgg
     7021 cgttatcttt acggtgttaa acccggcgca acgcgtgtcg atcttgacgg caacccatgc
     7081 ggtgagctgg acgagcaaca tgtagagcat gctcgcaagc agcttgaaga agcgaaagcg
     7141 cgtgttcagg cacagcgtgc tgaacagcaa gcgaaaaaac gcgaagctgc cgcagctgct
     7201 ggtgagaaag aagacgcacc gcgccgcgaa cgcaagccac gtccgaccac gccacgccgc
     7261 aaagaaggcg ctgaacgtaa acctcgtgcg caaaagccgg tagagaaagc gccaaaaaca
     7321 gtaaaagcac ctcgcgaaga acagcacacc ccggtttctg acatttcagc tctgactgtc
     7381 ggacaagccc tgaaggtgaa agcgggtcaa aacgcgatgg atgccaccgt attagaaatc
     7441 accaaagacg gcgtccgcgt ccagctgaat tcgggtatgt ctttgattgt gcgcgcagaa
     7501 cacctggtgt tctgaaacgg aggccgggcc aggcatgaac atgtttttta ggcttaccgc
     7561 gttagctggc ctgcttgcaa tagcaggcca gaccttcgct gtagaagata tcacgcgtgc
     7621 tgatcaaatt ccggtattaa aggaagagac gcagcatgcg acggtaagtg agcgcgtaac
     7681 gtcgcgcttc acccgttctc attatcgcca gttcgacctc gatcaggcat tttcggccaa
     7741 aatctttgac cgctacctga atctgctcga ttacagccac aatgtgctgc tggcaagcga
     7801 tgttgaacag ttcgcgaaaa agaaaaccga gttaggcgat gaactgcgtt caggcaaact
     7861 cgacgttttc tacgatctct acaatctggc gcaaaagcgc cgttttgaac gttaccagta
     7921 cgctttgtcg gtactggaaa agccgatgga tttcaccggc aacgacactt ataaccttga
     7981 ccgcagcaaa gcgccctggc cgaaaaacga ggctgagttg aacgcgcagt gggacagtaa
     8041 agtcaaattc gacgagttaa gcctgaagct gacaggaaaa acggataaag aaattcgtga
     8101 aaccctgaca cgccgctaca aatttgccat tcgtcgtctg gcgcaaacca acagcgaaga
     8161 tgttttctcg ctggcaatga tggcgtttgc gcgtgaaatc gatccgcata ccaactatct
     8221 ttccccgcgt aataccgaac agttcaacac tgaaatgagt ttgtcactgg aaggtattgg
     8281 cgcagtgctg caaatggatg atgactacac cgttatcaat tcgatggtgg caggtggtcc
     8341 ggcagcgaag agtaaagcta tcagcgttgg tgacaaaatt gtcggtgttg gtcaaacagg
     8401 caagccgatg gttgacgtga ttggctggcg tcttgatgat gtggttgcct taattaaagg
     8461 gccgaagggc agtaaagttc gtctggaaat tttacctgct ggtaaaggga ccaagacccg
     8521 tactgtaacg ttgacccgtg aacgtattcg tctcgaagac cgcgcggtta aaatgtcggt
     8581 gaagaccgtc ggtaaagaga aagtcggcgt gctggatatt ccgggcttct atgtgggttt
     8641 gacagacgat gtcaaagttc aactgcagaa actggaaaaa cagaatgtca gcagtgtgat
     8701 catcgacctg cgtagcaatg gcggtggggc gttgaccgaa gcggtatcgc tctcaggtct
     8761 gtttattcct gcgggtccca ttgttcaggt ccgcgataac aacggcaagg ttcgtgaaga
     8821 cagcgatacc gacgggcagg tgttctataa aggcccactg gtggtactgg ttgaccgttt
     8881 cagtgcttcg gcttcagaaa tctttgccgc ggcaatgcag gattacggtc gtgcgctggt
     8941 tgtgggtgaa ccgacgtttg gtaaaggcac cgttcagcaa taccgttcat tgaaccgtat
     9001 ttacgatcag atgttacgtc ctgaatggcc agcgctgggt tctgtgcagt acacgatcca
     9061 gaaattctat cgcgttaacg gcggcagtac gcaacgtaaa ggcgtaacac cagacatcat
     9121 catgccgacg ggtaatgaag aaacggaaac gggtgagaaa ttcgaagata acgcgctgcc
     9181 gtgggatagc atcgatgccg cgacttatgt gaaatcagga gatttaactg cctttgaacc
     9241 ggagctgctg aaggaacata atgcgcgtat cgcgaaagat cctgagttcc agaacatcat
     9301 gaaggatatc gcgcgcttca acgctatgaa ggacaagcgc aatatcgttt ctctgaacta
     9361 cgctgtgcgt gagaaagaga ataatgaaga tgatgcgacg cgtctggcgc gtttgaacga
     9421 acgctttaaa cgcgaaggta aaccggagtt gaagaagctg gatgatctac cgaaagatta
     9481 ccaggagccg gatccttatc tggatgagac ggtgaatatc gcactcgatc tggcgaagct
     9541 tgaaaaagcc agacccgcgg aacaacccgc tcccgtcaag taatatcaat caggcacaag
     9601 aaattgtgcc tgatttttta acagcggcaa gatgccgtaa atcagatgct acaaaatgta
     9661 aagttgtgtc tttctggtga cttacgcact atccagactt gaaaatagtc gcgtaaccca
     9721 tacgatgtgg gtatcgcata ttgcgttttg ttaaactgag gtaaaaagaa aattatgatg
     9781 cgaatcgcgc tcttcctgct aacgaacctg gccgtaatgg tcgttttcgg gctggtactg
     9841 agcctgacag ggatacagtc gagcagcgtt caggggctga tgatcatggc cttgctgttc
     9901 ggttttggtg gttccttcgt ttcgcttctg atgtccaaat ggatggcatt acgatctgtt
     9961 ggcggggaag tgatcgagca accgcgtaac gaaagggaac gttggctggt caatactgta
    10021 gcaacccagg ctcgtcaggc ggggatcgct atgccgcaag tggctatcta ccatgcgccg
    10081 gacatcaacg cttttgcaac cggtgcgcgc cgtgatgcct ctctggttgc tgtcagcacc
    10141 ggtttgctgc agaacatgag cccggatgaa gccgaggcgg taattgctca cgaaatcagc
    10201 cacatcgcca atggtgatat ggtcaccatg acgctgattc agggcgtggt gaacaccttc
    10261 gttatcttta tttcccgtat tctggcgcag cttgccgcgg gttttatggg cggaaatcgt
    10321 gatgaaggtg aagagagcaa cggcaacccg ctgatctact ttgcggttgc aacggttctg
    10381 gaactggtgt ttggtattct ggcgagcatt atcaccatgt ggttctcgcg tcatcgtgaa
    10441 ttccatgctg atgccggttc ggcaaaactg gttggtcgcg agaaaatgat tgccgcgctg
    10501 cagcgcctga aaaccagcta tgaaccgcaa gaagcaacca gcatgatggc tttctgcatt
    10561 aacggtaagt cgaaatcgct cagtgagttg ttcatgactc acccgccgct ggataaacgt
    10621 attgaagctc tgcgtacggg tgaatacctg aagtaatatg aagtaaatac taaaaagcgc
    10681 gtcctgatcg acgcgctttt tttatgccct ggctcgtggc tgagtgatac gtaaaccact
    10741 gacacaggca gcaatcactg ccagaatagc cgcagccatc agcgagacgt gcgtaccatt
    10801 atcaccaaac tgatttagca tcagcgccac cagcgccgcg ccactactct gacccagtag
    10861 acgagccgtt cctaacatgc cactggcccc accgctacgt tcgcgcggag cggaggtaat
    10921 aatggtgtgg ttattgggtg actggaataa gccaaatcca gcgccacata agatcatcgg
    10981 ccagataata ttgatatccg caggtgacgc gggcagcaga accagggaaa aaagccccgc
    11041 agccatgatg aacaacccta aagcacccag caatcctgca tgtacgcgtt caatcaaata
    11101 gcctgccagc ggagccatca ccatcgttgc taacggccac ggtgtcagaa gtaaacctgt
    11161 ttcgacttca ctacgcccga gcacggtttg caggtaaaag ggcagggaaa ccattgccag
    11221 catttgtgcg cagaaagagc aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg
    11281 cagtaaatcc accggtagca gcggtacggg aagagaaagc tggcggcgaa taaagaaaat
    11341 accaacaaca accattacca ccagttccgc agcaattaac gtcagcgatt gcccctgagc
    11401 gaaaccactc aacgcagtga taagcaggcc gaaggttaac gcgttcatca cggcgctggg
    11461 caggtcgaaa cggggtttac tggcgcgaga accattgggt ggcagaaaac gcatcgccag
    11521 aagcagggcg ataataccta acggtacgtt gattaaaaat aaccatttcc aggatgcgat
    11581 ggagaggatt gctgcagcaa ttgtcggccc ggcagcagaa gagacggcaa caataaacga
    11641 gtttatgccc atccctctcc ccagaaaacg ttgtggatag atcaggcgga taagtgcggt
    11701 attaacgctc atcaacgctg caccgccgaa accttgtatg acacgcgcaa gggtgagcat
    11761 ttgcagcgaa tcagaaaggg cgcagaacag tgaagacaac agaaaaacga ccagaccgca
    11821 tttataaata cgtcgatagc caaacatatc gcccagaaac gaaaacgaga gcagggagat
    11881 gacaatggcg atttgatagg cgttcactac ccagatggaa ctggctggtg tggcatgaag
    11941 gtccgtggcg attgttggca gggcgacgtt ggcgattgcg ccgtcaagga cagccatcga
    12001 aataccaatc acaatggtta atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc
    12061 ctgaactttt ggcataggaa ttttatatct ttggtgaata atcaacaggg gtatgattct
    12121 agcactgata ttcacggtgg atgtcgcaga attgtaacta accggcaaaa gatggattgc
    12181 gaccaccctg gtgcgaaatt ataataaaaa acagttctga tttttataaa acactcgcaa
    12241 tgaggtgata aatggctaac gcagatctgg ataaacagcc tgattctgta tcttccgtgc
    12301 taaaagtttt tggcattttg caggcgctgg gtgaagagcg cgaaataggg ataaccgagc
    12361 tgtcgcagcg cgtcatgatg tcaaaaagca ccgtttatcg ctttttacag accatgaaaa
    12421 ccttaggtta tgtggcgcag gaaggggagt cggagaaata ttccctgacc ctgaaattgt
    12481 ttgaactggg cgctcgcgcg ttacaaaacg tcgatttaat tcgtagcgca gatatccaga
    12541 tgcgtgagat ctcccgcctg accaaagaaa ctatccacct cggcgcactg gacgaagaca
    12601 gtattgttta cattcacaaa attgactcta tgtacaattt gcgcatgtat tcacggattg
    12661 ggcgtcgtaa tccgctgtac agcaccgcga ttggtaaggt actgctggca tggcgcgatc
    12721 gcgatgaagt gaagcaaatt cttgagggcg tggagtataa acgcagtacc gagcggacca
    12781 tcaccagtac agaagcgtta ttacccgttc tggaccaggt gcgcgagcag gggtatggcg
    12841 aagataacga agagcaggaa gaagggctgc gatgcattgc ggtaccggta tttgatcgct
    12901 ttggcgtggt cattgccggt ttgagcatct ccttcccgac gttgcgtttc tctgaagagc
    12961 gtttacagga atatgtcgca atgttgcata ccgcagcgcg caaaatttct gcccaaatgg
    13021 gttatcacga ctatccgttc tgatgagagt aagaacctgt cggaatatca aacagacagg
    13081 ttctttattt agcatgagaa aaataaagtt gaaggtgcgt tatattaaac gcgcttgcta
    13141 taagaatatt ttactcatga gtgagaatct ggttatttat tacccttaac cattatcgac
    13201 cacgatattg cttttacgta acagtgggca atctgttatc cccaagaaac cacttttagt
    13261 gtgcaagtat tgtaccgtgc tggtgcctct ggccgtcaga taggtacatt gcaaacctaa
    13321 tcctgcggca ttctctttgc ttccaatcaa aacgccatat ccgctgagta ataatcctat
    13381 ccataccagt gctatcagca taactgtgcg aatgatgaat cgcattacaa tctcttctct
    13441 ttttatgttc gcttaatcgt agcggcaata tgcgctgaag caagctactc attccgaaaa
    13501 agcacgaata tcgacatagt taggcgctgt ttaactaacg catgctagtt taatgacata
    13561 aggtaggtga agcggagatt ggagtgaaaa agtttcgatg ggtcgttctg gttgtcgtgg
    13621 tgttggcttg cttgctgctt tgggcgcagg tattcaacat gatgtgcgat caggatgtac
    13681 aatttttcag cggaatttgt gccattaacc agtttatccc gtggtgatat cattttttca
    13741 gcactagatt ttcttcctct ccagcagtgg tagaatagcg cctctactca gaggtggtga
    13801 aatgaacgaa gttgtaaatt caggcgtgat gaacattgcg tctttggttg tatcggtggt
    13861 ggttcttctt atcgggctca tcttgtggtt ttttatcaat cgtgccagtt cacggactaa
    13921 cgaacagatt gaactgcttg aggcgttgct ggatcagcaa aaacgtcaaa atgcactgtt
    13981 acgtcgtttg tgcgaagcaa acgaaccaga gaaagcagat aaaaagacca ttgagagtca
    14041 aaaatcggtt gatgacgaag atattattcg cctggtcgcc gaacgataat ccttcctcaa
    14101 tcttgagtaa ccccgtcgtt gaagggttac tcaattcatc ctgattatac gcactgtcat
    14161 ggtgcaactg cttcaaatta aaactctggt ctgacttcaa ataattatca tattatttat
    14221 ttgcgtaacg ggatatcaga aaaagatatt ttcctcatta atttattagg atgtttacat
    14281 cagatttgtg attaagcgtg gtattattta ttagcgaaac gtttctctct tgattttttt
    14341 gctcactcat caatttttct tattttaaat ttacaatccc tttggggatt gacttctctt
    14401 tagggtaatt gatagccgtt aactgactgt tttatgagaa aaagtgatat aactttttat
    14461 tcattgcata gcagaaaatg tgatattgca cgcactatgt aataacttct ccctctggcc
    14521 tggaacaact gaacttattg aactatgtta gaaactacgc cagtttaagt atctgcctga
    14581 actggcaagg ttaagcacaa tgatatatcg gcgcgtattc cgttgcataa gtgtgcaaaa
    14641 aaagtggaag acgtatcgag atttgtgcgt ctgatcgaga catgtttaaa aatggcttgc
    14701 cataattaac gttgtatgtg ataaaagatt tcgggttaaa cgaggtacag ttctgtttat
    14761 gtgtggcatt ttcagtaaag aagtcctagg taaacacgtt gacgttgaat accgcttctc
    14821 tgccgagcct tatattggtg cctcatgcag taatgtgtca gttttatcta tgttatgcct
    14881 gcgggcgaag aaaacaatct aaggaatttt tcaaatggca aagattaaag gtcaggttaa
    14941 gtggttcaac gagtctaaag gttttggctt cattactccg gctgatggca gcaaagatgt
    15001 gttcgtacac ttctccgcta tccagggtaa tggcttcaaa actctggctg aaggtcagaa
    15061 cgttgagttc gaaattcagg acggccagaa aggtccggca gctgttaacg taacagctat
    15121 ctgatcgaat ccactgatct gaagtgtgaa tacgcttcaa tctcgctata aagcctcgtc
    15181 gaatgcgagg ctttttacta tgctttatct tcgctcctgg cgttcggata tttgccagcc
    15241 acgtgattcg cgttacactt gcggccttta gtatcctgcc ggagttgtca tgtcgttttc
    15301 ctgtccactt tgccatcagc ctctttcgcg tgaaaaaaac agctatatct gtccccaacg
    15361 acatcagttt gatatggcga aagaagggta tgtcaatctg ctgcccgttc agcataaacg
    15421 gtctcgtgat ccgggcgaca gcgcggaaat gatgcaagca cgccgcgcat tcttagatgc
    15481 cggacattat cagccgctgc gtgatgcaat tgtcgcccaa ctgcgggaac ggcttgatga
    15541 aaaggccacg gcggtgctgg atattggctg tggtgaaggg tattacacac acgcatttgc
    15601 cgatgcgttg cccgaaatca ccacgtttgg cctggatgtc tcgaaggtag cgataaaagc
    15661 ggcggcgaaa cgctatccgc aggtcactct ttgtgtcgct tccagccacc gtttgccgtt
    15721 ttccgatacc agtatggacg ccataatacg tatttacgcg ccgtgtaaag cagaagaatt
    15781 agcacgagta gtgaagcccg gcggctgggt cattactgcc acgccgggac cgcgacattt
    15841 gatggagctg aaggggctga tttacaatga agtacatctt catgcacctc atgcagaaca
    15901 actggaaggt tttacattac agcagagtgc ggagttgtgt tatccgatgc gtcttcgcgg
    15961 tgatgaagcc gtcgcattat tgcagatgac gccgtttgcc tggcgtgcga agccagaagt
    16021 ctggcaaaca ctggcagcaa aagaagtgtt cgactgccag acggacttta atattcacct
    16081 ctggcagcgt tcttattaac cgtggaagtg cgtccagagg atctggacgc cgatgccgat
    16141 cagcaccagc ccgccgagaa tttccgcttt tttcccaata attgagccga taaagcgacc
    16201 aaccatcatc cctaatgtcg acataatcaa ggttgcacaa ccaatggcca atgcggtcgc
    16261 gataatgttg acctgaagga aagcaagacc aacacccaca gccatggcat ccaggctggt
    16321 ggcaatcgcg gtggttacca gtagccagaa accgtgccga cggcgcggct cttcatcttc
    16381 atcatctgag ccacgaaaac cctcaataat cattcgcccg ccgaggaata tcagcagcac
    16441 aaacgcaatc cagtgattcc attcaaggac aaaccggctg gctaacatgc ccattcccca
    16501 gccgatcagc ggcgtcaggg tttcgacggc accaaaaata aggccggttc gcaatgcttc
    16561 agaaaatttc ggtttatgga ggggggcacc tttaccgatt gatgcagcaa atgcatccat
    16621 cgacatacca aacgcaagaa gaacagtagc agtgatattc atgacaacgt cctgaccggg
    16681 gtatccataa aacacatcat cgcccccagc aaacatgcat acatgaacaa caagccgcga
    16741 tgggttttcg cgttcatgca ggcatagcga ttgatgtgca tatggtctcg cctgactgaa
    16801 agtcttcagt ccgtacgtgc cacgttttgc aacgagtatg ttgacacgta catttccgga
    16861 atctggaaat cggctactcc ccaaatgagg gcgcaacctt aacatatttt gagaatataa
    16921 aacaactgtt ggaatatatt atttcattgc tttattgata acgattttca tttatgttta
    16981 tatatcaatg aaacgttaaa ataaataaca aataagggaa gagataatat agcctaagct
    17041 atgtctgatt attaaaaata cactaaacat agctttggct aaattcataa gttattgagt
    17101 tgata
//
LOCUS       DADLTF010000081        16825 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.081, whole genome shotgun sequence.
ACCESSION   DADLTF010000081 DADLTF010000000
VERSION     DADLTF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.081"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(186..872)
                     /gene="kptA"
                     /locus_tag="JN749_002533"
     CDS             complement(186..872)
                     /gene="kptA"
                     /locus_tag="JN749_002533"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HAY7368906.1"
                     /translation="MWKRFCAGLSCRKFINPVTMTLVNKNRKSRDENAMAKYNEKELA
                     DTSKFLSFVLRHKPEAIGIVLDREGWADINKLILCAQKAGKRLTRALLDTVVATSDKK
                     RFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQFLYHGTASRFLDEIKKQGLIAGDRH
                     YVHLSADEATARKVGARHGSPVVLTVKAQEMAKRGIPFWQAENGVWLTSTVAVEFLEW
                     SFMPTGVQKQ"
     gene            1019..1702
                     /locus_tag="JN749_002534"
     CDS             1019..1702
                     /locus_tag="JN749_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HAY7368907.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1699..2160
                     /locus_tag="JN749_002535"
     CDS             1699..2160
                     /locus_tag="JN749_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368908.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2173..3345
                     /gene="iadA"
                     /locus_tag="JN749_002536"
     CDS             2173..3345
                     /gene="iadA"
                     /locus_tag="JN749_002536"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAY7368909.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTLFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3410..4321
                     /gene="hypT"
                     /locus_tag="JN749_002537"
                     /gene_synonym="qseD"
     CDS             3410..4321
                     /gene="hypT"
                     /locus_tag="JN749_002537"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HAY7368910.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4314..4706)
                     /gene="iraD"
                     /locus_tag="JN749_002538"
     CDS             complement(4314..4706)
                     /gene="iraD"
                     /locus_tag="JN749_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HAY7368911.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4703..4786)
                     /locus_tag="JN749_002539"
     CDS             complement(4703..4786)
                     /locus_tag="JN749_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide"
                     /protein_id="HAY7368912.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            5379..6209
                     /locus_tag="JN749_002540"
     CDS             5379..6209
                     /locus_tag="JN749_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7368913.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(6350..7123)
                     /gene="uxuR"
                     /locus_tag="JN749_002541"
     CDS             complement(6350..7123)
                     /gene="uxuR"
                     /locus_tag="JN749_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HAY7368914.1"
                     /translation="MKSVTSVQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7338..8798)
                     /locus_tag="JN749_002542"
     CDS             complement(7338..8798)
                     /locus_tag="JN749_002542"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HAY7368915.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLTLGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8879..10063)
                     /gene="uxuA"
                     /locus_tag="JN749_002543"
     CDS             complement(8879..10063)
                     /gene="uxuA"
                     /locus_tag="JN749_002543"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAY7368916.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10403..11746
                     /gene="gntP"
                     /locus_tag="JN749_002544"
     CDS             10403..11746
                     /gene="gntP"
                     /locus_tag="JN749_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HAY7368917.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11997..12899)
                     /gene="fimH"
                     /locus_tag="JN749_002545"
     CDS             complement(11997..12899)
                     /gene="fimH"
                     /locus_tag="JN749_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HAY7368918.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12919..13422)
                     /gene="fimG"
                     /locus_tag="JN749_002546"
     CDS             complement(12919..13422)
                     /gene="fimG"
                     /locus_tag="JN749_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HAY7368919.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(13435..13954)
                     /gene="fimF"
                     /locus_tag="JN749_002547"
                     /pseudo
     CDS             complement(13435..13954)
                     /gene="fimF"
                     /locus_tag="JN749_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
     gene            complement(13964..16600)
                     /gene="fimD"
                     /locus_tag="JN749_002548"
     CDS             complement(13964..16600)
                     /gene="fimD"
                     /locus_tag="JN749_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HAY7368920.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSIMAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQFYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(16667..>16825)
                     /locus_tag="JN749_002549"
     CDS             complement(16667..>16825)
                     /locus_tag="JN749_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone FimC"
                     /protein_id="HAY7368921.1"
                     /translation="ELNAGTRVLENALVPPMGESTVKLPSDAGSNITYRTINDYGALT
                     PKMTGVME"
BASE COUNT         4086 a         4332 c         4095 g         4312 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaacaatgg ggattgttat
     1021 gacacaacaa ggggatgctg tagccgggga gctggcgaca gaaaaagtgg gcattaaagg
     1081 gtatctggct ttttttctca ccataatctt tttttccggt gttttttccg gaacagacag
     1141 ctggtggcgc gtttttgatt tttccgttct gaacggatcg tttgggcaac tgcctggtgc
     1201 gaatggagcc accacctcat ttcgcggggt gggtggcgcg ggggctaaag atggctttct
     1261 ctttgcgctg gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga
     1321 tggtcttggt ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt
     1381 gggcattcca gggatctgct cgctggcact gatcgctaac ctgcaaaaca cggatgccgc
     1441 cgccggtatg accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat
     1501 tttcgccgca tatcaaacca gcggtagcgc catcatcacc aactacttct cttccggcgt
     1561 cgcggtgttt gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt
     1621 taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc
     1681 aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc
     1741 gatggcgcgc gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg
     1801 gcgtttgtca tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc
     1861 tgtgaacccg taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc
     1921 gcattaatga gtatgggcgg cgcagtcggt gttgccgcca gtctggcaac cgctgacgca
     1981 ttaaccggcc acgatgtcac cgttttactg cccgcaatgt atttaatggg caacccggtg
     2041 caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc
     2101 attacggtct gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa
     2161 caaggagtta ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat
     2221 ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc
     2281 gccgttgcca gcaatatccc ttctgacatt gtaccgaact gcacggttgt cgatctcagc
     2341 gggcagatcc tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc
     2401 gaagcaggtc ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc
     2461 gtcacgtcag tggttggtct gctgggcacc gactctatct ctcgccacct ggaatccctg
     2521 ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct
     2581 tatcatgtcc cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat
     2641 cgtgtgattg gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat
     2701 cacctggcta atatggcggc agaatcccgc gttggcggtt tgctcggcgg taaacctggc
     2761 gtcaccctgt tccacatggg cgacagtaaa aaggcgttaa agcctattta tgacctgctg
     2821 gaaaactgcg atgtgccgat cagcaagctg ctgccgaccc acgttaaccg caacgtaccg
     2881 ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc
     2941 attgacgaac cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattctg
     3001 ctggcacgcg tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa
     3061 gggaatttaa cccatatcgg tgttgcaggt tttgaaacgt tgctggaaac cgtgcaggtg
     3121 ctggtcaaag actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta
     3181 gccggtttcc ttaacatgag cgggaaaggc gaaattctgc caggcaatga cgcagactta
     3241 ctggtcatga cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc
     3301 aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg cttaaacggt tgtcagccgc
     3361 cctaatggac gtagaatgcc cccaagggcg gctgacagag taaaacgtaa tggatgactg
     3421 tggtgcggtt ttgcataata ttgaaaccaa atggctttat gattttctga ctctggaaaa
     3481 atgccgcaat ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg
     3541 ccgcatccgt gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc
     3601 actgcaactc tcagaacaag ggaaaatctt ccattcgcag atccgccatc tgttgcaaca
     3661 gttagaaagc aaccttgcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa
     3721 gatagccgct gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc
     3781 gccgctcttt acttgggcaa ttgaagccat tgatgtcgat gaagcggtcg ataaactgcg
     3841 tgaagggcaa agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt
     3901 tgaccacatt cgcttatttg aatctcaatt gttccctgtc tgcgccagtg acgaacacgg
     3961 agaagcactt tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc
     4021 ctacatgggg cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcgc
     4081 cttttttgtc tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat
     4141 tgcctggctg ccggagtatg ccatccaaca agaaattcgc agcgggcagc tcgttgtgct
     4201 gaatcgggac gaacaggtga tcccgattca ggcctacgca taccggatga atacccgcat
     4261 gaatccagtt gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg
     4321 acattctcaa gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc
     4381 ggttcatcgt cgtggtacag cattatctca aggctgatcg ccagagtgtg gcagtacgct
     4441 tctttcaaat taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc
     4501 agctgacggc aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg
     4561 ggcatgcctg aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtaagg
     4621 agatcgctgc ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa
     4681 acagcctgaa gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg
     4741 cttgccctga acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt
     4801 gttcattaca tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc
     4861 ttacttgcct atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc
     4921 atcaaactca aaaaaaaacc gctagtatat tctgaatatt ccgaatagtt acgtagatga
     4981 aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc cgcataatca
     5041 gccatgtatt atttgaaaca attagtttgt aaataatata aattgataac atatcaaata
     5101 catcaaaaat accattcaca ggcattatta atatagacct caatatagca acatcaaatt
     5161 ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat gagcgttttt
     5221 tgatacttta tagtgtttcc taccctttaa tatcactgtg tagagttgat atcactacaa
     5281 cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt tatctatttt
     5341 tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca ttaagcaatg
     5401 ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa gtcgacagtg
     5461 agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat cttctgcctc
     5521 ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt agaaaactta
     5581 atattggaaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga gaattcaatg
     5641 gtattacaac aacaggacgt atgctctatc gaaacttgaa aatcacctca gctttagacg
     5701 aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca gaaggtttac
     5761 ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca gttcatgaaa
     5821 taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata ggatatattt
     5881 caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga attgatatct
     5941 atattgatca gataataact gtctcagggg tagcaaattc ttccggattc gcccttgccg
     6001 cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata gaagcctatc
     6061 ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt gatgaagatg
     6121 cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt gaattaaaaa
     6181 atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact aatattcagg
     6241 ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt gcgacttctt
     6301 aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt caggcgtcga
     6361 ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg tcaacgttcg
     6421 agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc agcttcgccg
     6481 ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc aaccactctt
     6541 tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga ttgttttccc
     6601 gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt gcttcagcaa
     6661 tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg gaagccagtt
     6721 cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg gtagcctgca
     6781 gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac agctcaaaag
     6841 gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg cctgagctat
     6901 caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc tccagcatga
     6961 tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt tcacgctccg
     7021 gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgcgg atcatcgccc
     7081 cgacttcctg gtaaggtctt tgcacagagg tgacagattt catggtgttg tgtccgttag
     7141 gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc ctacaacggg
     7201 caattttacg gcccccgtta atctcaaact ataccattag cactggaagt tggcacgtac
     7261 aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac cagtaagtct
     7321 gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg ttcgcacagc
     7381 tgctgatatg ccgccgtcac cgcgccaaca aagtcggcat tctgcggcag atcatcggca
     7441 aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata ctgcgcgtta
     7501 atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc ctgctcatcc
     7561 acgccctggg tgtaacgcat ccagccagcc acgcccagcg tcaggtgacg ccagctgccg
     7621 ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt ctggctgccg
     7681 tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg ctcgatcagc
     7741 agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg cgcttgttcc
     7801 tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt gtcggcaatg
     7861 gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc gttcagcata
     7921 cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgcgcc cactttatcc
     7981 cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa cggttcgcag
     8041 gcaatggcgc atgggtcgta aacacccagc tggtcagcaa tttcctgtaa ggtttctggc
     8101 gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt ctcttcaatc
     8161 catgccgcta gttgcgggtc acgcgcctga gccagcccca gtaccgcgac cttcgccaca
     8221 tgaccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag ccctttttca
     8281 cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc agtcgggttt
     8341 tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc cgcatcagcg
     8401 cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg cgccatcgcg
     8461 ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat cgaaccgata
     8521 attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag ttgctgtttt
     8581 ttcaggtttt cgatcagcat acggtcgttg cctggcatca ggttaacttc gcagatgccc
     8641 cagtcgctgt cggtgctttc cagcagatga tgggtataca acgcctggtg cgcgcggtga
     8701 aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc ccatgacggg
     8761 cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag aatccgtctt
     8821 cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc ggtgggtatt
     8881 aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg gccaggcctt
     8941 tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg tccagcatct
     9001 gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct ttacgacggt
     9061 gttcttcttc aacaatcgct ttcaccactt cgtacatatc aacgtcaccg ttcaggtgcg
     9121 ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga tgggtgaagt
     9181 aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca cgcacgccgt
     9241 aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca accatccact
     9301 gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc ggcggatcgt
     9361 ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc gctttcagga
     9421 agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc tccaggtgct
     9481 tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca atgatgttac
     9541 gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct gcctgagcaa
     9601 tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc atttcgaatg
     9661 cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc acgtattcga
     9721 ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg cgaatgccac
     9781 actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca tagttgccag
     9841 tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac accaggcctg
     9901 cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact tcgccgttcg
     9961 ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca tcagctaaag
    10021 aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt cctctttcac
    10081 atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta tccggatgaa
    10141 caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt ctaccttttg
    10201 gtaagacatg tcaacccgaa ctctgaaatt ggttaaccac atcacaagaa tttcactctc
    10261 atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca gaggatgtga
    10321 cattcatcgc aacaatggtt gaccaatttg catgacatgt cgcccaaaat aacaacggtt
    10381 caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg tattcggcat
    10441 tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg ctttgttggt
    10501 ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc tgcacaccat
    10561 gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt tcggtgcggt
    10621 catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata cgctgctggc
    10681 gcgtctaggt ctgcgctatg ttcagctgtc ggtgattatc atcggcctta ttttcggtct
    10741 ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg ttattgccgc
    10801 cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg ccactaccgc
    10861 acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt atggcgcgga
    10921 tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa tctgcgcagg
    10981 tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat tcctgaaagc
    11041 agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc tggtgccgct
    11101 gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg taaaagatac
    11161 ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt ttattgcgat
    11221 ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt gggtgatgaa
    11281 cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg gtgcgggtgg
    11341 cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca tgttgatgtc
    11401 ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa ttcgtctggc
    11461 gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg ctgcaatcct
    11521 tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac tggcgacggc
    11581 tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt tcaaaggtta
    11641 ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc tggtcaactc
    11701 cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga acaccagagc
    11761 ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg acagtcccct
    11821 cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc gctttgttta
    11881 tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt gcaggttttg
    11941 taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta atttttttat
    12001 tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac ctgccctccg
    12061 gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac tgctcctaac
    12121 gataccgtgt tattcgttgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc
    12181 tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg
    12241 ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag aggaattggc
    12301 actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcacaaccg
    12361 ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg aaaatcatcg
    12421 ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc agctttaatc
    12481 gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc
    12541 gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg atagctactg
    12601 ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc cgagcctcgt
    12661 tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc
    12721 gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt tacataaaca
    12781 ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca ggcgaatgac
    12841 caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc tcgtttcatt
    12901 acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt
    12961 tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga
    13021 ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg tgttgccact
    13081 gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt
    13141 gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg gacaattagt
    13201 caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa gactgaaaga
    13261 ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg gtttggcgac
    13321 gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac ttgcgagcgc
    13381 caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct ccagttactg
    13441 atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag gcacctgtgt
    13501 cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca gggtcgtcca
    13561 cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat tcagaagctg
    13621 tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg
    13681 gctatctgca acgccggtaa acccaacttt tacggcagaa acagggtgac agcaaaatac
    13741 gaaatggaac gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca
    13801 tcagatcaac agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat
    13861 agccgcggat agtaatcgtg ctatccgcag ccaaaacgcg acttaccgcc agccacaaaa
    13921 aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg
    13981 atagctgggt taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt
    14041 gagcattttc ctcttctccc catttcacct gaacttttcc cgctaaaggc attccgctga
    14101 ggtaaaactg accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca
    14161 tcgccccaaa cggcagcggc ttattattgt gagttagcgt catgagcagt tttatcccaa
    14221 cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg
    14281 cgttatctaa atcgacgtta tcagccaagg tattggtatc cagcgccact ctattttccc
    14341 gatattcagt ggcataaggc agcacggcat aaccacgcca gtcggtacgc acccccgtct
    14401 ggttttcgac ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta
    14461 acggctgccc cagcgttacg ccattggcat gagccaatac cccaccgctg actccgtaat
    14521 agagctgctt aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc
    14581 gataattcag cgtggcgtag cctgtacttc cgctattacc atcgcctccc ccggcatagc
    14641 cggtttgcac gctataactg aggttgttgt cttccagcaa cgtaccgtat acaccagcca
    14701 gattggtcat ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc
    14761 actgagattt actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttaagcgcta
    14821 acatctgatc ccgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt
    14881 tgatatcttc gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc
    14941 cccaataagt ttgatggcta ccactcaaat acagtgttga tgtgcgcccg agttgctgag
    15001 taacggtgag ctgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt
    15061 tcggcttaac ctgaataact ccatcctgtg tttcgatgtt gtagccattc attcgactgt
    15121 atgttgtatc agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa
    15181 tattcgtgcc tgattcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat
    15241 gctgactgtc atcgggaagt gtggaattag cctgcgtcat atccacagac agagcgccca
    15301 gtgcctccat gtttttcccg ataccgaaat taaaagcacg ataacgatcc gccagttgcg
    15361 ttccaccata tattgtccag ccagccggaa ggccgtggag taatgtactc tggaaaaagc
    15421 ggggtttttc ctgctgcgca tttccactac ggtattctcc tgccataatg gaataacgag
    15481 tatgcccttc acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc
    15541 tgccgtcagc ctctttgatc gttacctgca agtcaccact attacctgcg gcatagatat
    15601 cgttgatggt aaaaggcccc ggtggcaccg tactattata aatgccatat ccattttgtt
    15661 taatagtgac ctgtgcagta ccatgagcaa taccgtggat caccggggca aatcctcttt
    15721 ggctatcggg taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat
    15781 cgaaaatatc gccctgggta taaccatcac ccaacgtcag ccgggaacgt aaaggaatta
    15841 tgtctcgctc aatccaggta atgatatgct gccatttatt tttgctacct gatgaactgt
    15901 cgctactgtt ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc
    15961 cactctgtag gtttaaatat gcataatggc tgttaccccc aatccgattc tgtacactat
    16021 ttccgctgaa attataattg agcaatccgg cattaatacc gggatcccat aactcaggag
    16081 gaatataacc acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttcagtcgct
    16141 gctgaccaac atccagatgc gcagtagcgt cctggaccat tgtggttaat ggcacacagg
    16201 catcatccgc cagcagattc ataccggcga cagaagccgt attcagcccc atactggcga
    16261 gttgcgcgcg tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga
    16321 catcacgcgt tgccatataa ccattattca aatagatatc gacgcgatac gtccctggcg
    16381 gtaattcttg cccattttca aaacgcgata aatcggccac agcctgggga tcatccgcta
    16441 aaaagcgcag attaaaatag aggtcggcag atgacaaagg tgcctgtgcg gcaaaagcac
    16501 aggcgacaac gagtcggaca aaaaaaccag ccaaacgatg cttacgaata tgcaagcatt
    16561 gtgtgtttcg ctggtaaagt cttaaattca gatatgacat tattcctccg gtttaaaatc
    16621 acccggcagt caattctttt tattcaggcg aaaaattccc cctgcgttat tccattacgc
    16681 ccgtcatttt gggggtaagt gcgccataat catttattgt tcggtaagta atattgcttc
    16741 ctgcatcaga aggcaattta accgtgcttt cgcccattgg aggcaccaat gcattttcaa
    16801 gaacccgggt tccggcattc aactc
//
LOCUS       DADLTF010000082        16809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.082, whole genome shotgun sequence.
ACCESSION   DADLTF010000082 DADLTF010000000
VERSION     DADLTF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.082"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..942
                     /locus_tag="JN749_002550"
     CDS             <1..942
                     /locus_tag="JN749_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HAY7368922.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
     gene            942..4091
                     /gene="mdtB"
                     /locus_tag="JN749_002551"
     CDS             942..4091
                     /gene="mdtB"
                     /locus_tag="JN749_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HAY7368923.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVALSTLLLSVLLWVFI
                     PKGFFPVQDNGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTN
                     PSLNSARLQINLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRT
                     QYQFTLQATSLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLG
                     ISMADVDNALYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGV
                     VPLSSIAKIEQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVD
                     ITTQFQGSTLAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGA
                     LLALMIAGSELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLL
                     RFRPILMTTLAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLL
                     FDRLVLWTKSRFARHEEEA"
     gene            4092..7157
                     /gene="mdtC"
                     /locus_tag="JN749_002552"
     CDS             4092..7157
                     /gene="mdtC"
                     /locus_tag="JN749_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HAY7368924.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLRITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            7158..8573
                     /locus_tag="JN749_002553"
     CDS             7158..8573
                     /locus_tag="JN749_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7368925.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            8570..9973
                     /gene="baeS"
                     /locus_tag="JN749_002554"
     CDS             8570..9973
                     /gene="baeS"
                     /locus_tag="JN749_002554"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HAY7368926.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            9970..10692
                     /gene="baeR"
                     /locus_tag="JN749_002555"
     CDS             9970..10692
                     /gene="baeR"
                     /locus_tag="JN749_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HAY7368927.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            10883..11215
                     /locus_tag="JN749_002556"
     CDS             10883..11215
                     /locus_tag="JN749_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HAY7368928.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            11362..12723
                     /locus_tag="JN749_002557"
     CDS             11362..12723
                     /locus_tag="JN749_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HAY7368929.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(13065..13382)
                     /locus_tag="JN749_002558"
     CDS             complement(13065..13382)
                     /locus_tag="JN749_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368930.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            13788..14687
                     /gene="yegS"
                     /locus_tag="JN749_002559"
     CDS             13788..14687
                     /gene="yegS"
                     /locus_tag="JN749_002559"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HAY7368931.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(14769..15548)
                     /locus_tag="JN749_002560"
     CDS             complement(14769..15548)
                     /locus_tag="JN749_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAY7368932.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(15648..16688)
                     /gene="gatD"
                     /locus_tag="JN749_002561"
     CDS             complement(15648..16688)
                     /gene="gatD"
                     /locus_tag="JN749_002561"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7368933.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(16736..>16809)
                     /locus_tag="JN749_002562"
     CDS             complement(16736..>16809)
                     /locus_tag="JN749_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7368934.1"
                     /translation="TGIFMTWRRARGFTKQEKAVLAE"
BASE COUNT         3886 a         4500 c         4407 g         4016 t
ORIGIN      
        1 accgggcttg gcaccattac cgccgctaat actgttacgg tgcgcagccg cgtggacggt
       61 caactgatgg cgttacattt ccaggaaggc cagcaggtca aagcaggcga tttactggca
      121 gaaattgacc ccagccagtt caaagttgca ttagcacaaa cccagggcca actggcaaaa
      181 gataaagcca cgcttgctaa cgcccgccgt gatctggcgc gttatcagca actggcaaaa
      241 accaatctcg tctcccgtca ggagctggat gcccaacagg cgctggtcag tgaaaccgaa
      301 ggcaccatta aggctgatga agcaagcgtc gccagcgcgc agttgcaact cgactggagc
      361 cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc aagttgatgt tggtaaccaa
      421 atctccagtg gtgataccac cggaattgtg gtgatcaccc agacgcatcc tatcgattta
      481 gtctttaccc tgccggaaag cgatatcgct actgtagtgc aggcgcaaaa agccggaaaa
      541 ccgctgatgg tagaagcctg ggatcgcacc aattcgaaga aattaagtga aggcacgctg
      601 ttaagcctcg ataaccaaat cgatgccaca accggtacga ttaaagtgaa agcacgcttt
      661 aataatcagg atgatgcgct gtttcccaat cagtttgtta acgcgcgcat gttagtcgac
      721 accgaacaaa acgccgtagt gatcccaaca gccgccctgc aaatgggcaa tgaaggccat
      781 tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac atctggtgac gccgggcatt
      841 caggacagtc agaaagtgga agtggtggaa gcccagagcg ccaccactcc ggaagagaaa
      901 gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg ttacccccga
      961 gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc acgctgctga
     1021 tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt tcggcgctgc
     1081 cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcc agcccggatg
     1141 tcatgacctc tgccgttacc gcgccgctcg aacgccagtt cgggcagatg tctggcctga
     1201 aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttgcag ttccagctaa
     1261 cattaccgct caatgtcgcc gagcaggaag tgcaggccgc gattaacgct gcgaccaact
     1321 tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg gcagatccgc
     1381 cgatcatgac gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg gaagatatgg
     1441 tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg cgtcggcctg gtgacgcttt
     1501 ccggcggtca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt gccgccctcg
     1561 gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac tcggcaaaag
     1621 gtagcctcga cggcccttcc cgtgcggtca cgctttccgc gaacgaccag atgcaatccg
     1681 ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccaatt cgtctgggcg
     1741 atgtagcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg gcgaacaaag
     1801 aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc tccaccgccg
     1861 acagcattcg gcagatgctg ccacagctca ctgagagtct gccgaaatcg gtgaaggtga
     1921 cagtgctttc cgatcgcacc accaatatcc gcgcatccgt cgatgatact cagtttgaat
     1981 tgatgatggc tatcgcgctg gtagtcatga ttatctacct gtttttgcgc aatattccgg
     2041 cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc gcggttatgg
     2101 tgtttctcga tttttcaatc aataacctga cactgatggc gttaactatc gccaccggat
     2161 tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc ccgctatatc gaaaaaggcg
     2221 aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat cggctttacc attatctcgc
     2281 tgaccttctc actgattgcg gtgttgatcc cactgctgtt tatgggcgat atcgtcgggc
     2341 gactgttccg cgaatttgct attaccctgg cggtagcgat tttgatctca gcggtggtgt
     2401 cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg ttgcgtaaac
     2461 agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc gcctatggtc
     2521 gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggca ctcagcacgc
     2581 tgctgcttag cgtggcactc agcacgctgc tgcttagcgt gctgctgtgg gtgttcattc
     2641 cgaaaggttt cttcccggta caggacaacg gcattattca gggcactttg caggcaccgc
     2701 aatccagctc ctttgccaat atggcccagc gacaacgcca ggtcgcggac gtgattttgc
     2761 aggatccggc agtgcaaagc ctgacctcat ttgttggcgt tgatggcact aacccgtcgc
     2821 tgaatagtgc acgtttacaa atcaacctca aaccgttgga tgaacgtgat gatcgggtgc
     2881 aaaaagtcat cgcccgtctg caaacggcgg tagataaagt gccgggcgtc gatctcttcc
     2941 tgcaaccaac gcaggacctg accatcgata ctcaggtcag ccgcacccag taccagttta
     3001 ccttgcaggc cacgtcactg gatgcgctca gtacctgggt gccacagttg atggaaaaac
     3061 tccagcaact gccacagctt tctgatgtct ccagcgactg gcaggacaaa gggctggtgg
     3121 cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg gatcagcatg gcggacgtcg
     3181 ataacgccct gtacaacgcg tttggtcagc gactgatttc cactatttat actcaggcta
     3241 accagtatcg cgtggtgctg gagcacaata ccgaaaatac cccaggcctc gcggcgctgg
     3301 ataccattcg cctgaccagc agcgacggtg gcgtggtgcc gttaagctca attgccaaaa
     3361 ttgagcagcg ttttgcgccg ctttccatca accatctgga tcagttcccg gtaacaacca
     3421 tctcctttaa cgtgccggat aactattcgc tgggcgatgc ggtgcaggcg attatggaca
     3481 ccgaaaagac gctgaatctg ccggtggata tcaccacgca attccaaggc agcaccctcg
     3541 ccttccagtc ggcgctgggc agcactgtct ggctgattgt cgcggcggtg gtggcgatgt
     3601 atatcgtgct cggcattctg tacgagagct ttattcaccc gatcaccatt ctctcgacgc
     3661 tacccaccgc aggggtcggc gcgctgctgg cattaatgat tgccggtagc gaactggatg
     3721 tgattgcgat tatcggcatt attttgctga tcggtattgt gaagaagaac gccatcatga
     3781 tgatcgactt cgcgctggct gctgagcgcg agcaaggcat gtcgccgcgc gatgcaatct
     3841 accaggcttg tctgttgcgt tttcgtccga tcctgatgac caccctggcg gctctgcttg
     3901 gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga actgcgtcgt ccgttaggta
     3961 tcggcatggt cggcggtctg attgtcagcc aggtgctgac gctgttcacc acgccggtga
     4021 tttatctgct gttcgaccgc ctggtattgt ggaccaaaag tcgctttgct cgccatgaag
     4081 aggaggcgta agtgaagttt tttgccctct tcatttaccg cccggtggcg acgattttac
     4141 tgtcggtcgc cattaccctg tgcggcatac tgggtttccg tatgctgccg gtcgccccgc
     4201 tgccgcaggt cgattttccg gtgattatgg tcagcgcctc gctgcccggt gcgtcgccgg
     4261 agacaatggc gtcttccgtt gccacgccgc tggagcgctc acttgggcgc attgccgggg
     4321 ttagtgaaat gacctccagc agttcgctcg gcagcacgcg cattattttg cagtttgatt
     4381 ttgaccggga tatcaacggc gcagcgcgtg atgtgcaggc ggcgatcaac gctgcacaaa
     4441 gtttgctgcc cagcgggatg cccagccgcc cgacctatcg caaagcgaac ccgtcggatg
     4501 cgccaattat gatcctcacg ctgacatccg atacttattc gcagggtgaa ctgtacgatt
     4561 tcgcctcgac gcagctggct ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg
     4621 tcggtggcag ctcgctgccc gccgtgcgcg tcgggctgaa cccgcaggcg ctgtttaatc
     4681 agggcgtgtc gctggacgac gtacgcactg ccatcagcaa tgccaacgtg cgtaaaccgc
     4741 agggggcgct ggaagatggc actcaccgct ggcagatcca gaccaacgat gaactgaaaa
     4801 ccgccgctga atatcagccg ctgattattc actacaacaa cggcggcgcg gttcgtctgg
     4861 gcgatgtggc gacggtgact gactcagtgc aggatgtgcg caacgccggg atgaccaacg
     4921 ccaaaccggc tattttactg atgatccgca aactgccgga agccaatatt atccagacgg
     4981 tagacagcat ccgggcaaaa ttaccagagt tgcaggagac cattccggcg gcgattgatc
     5041 tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc gctggaagaa gtcgagcaaa
     5101 cgctgattat ctcggtggcg ctggtgattc tggtggtctt tttattcctg cgctcgggtc
     5161 gcgccactat tattcccgcc gttgcggtgc cggtttcgct gattggtacg tttgcggcga
     5221 tgtacctgtg cggattcagt ctcaataatc tttcgctgat ggcgctcacc attgctactg
     5281 gtttcgtggt ggatgacgcc attgtggtgc tggaaaacat tgcccgtcat ctggaagcgg
     5341 ggatgaaacc actacaggcc gcgctgcaag gtactcgcga agtcggcttt acggtgctgt
     5401 cgatgagtct gtcactggtg gcggtgttcc tgccgctgct gttgatgggc ggattgccgg
     5461 gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc cattggtatt tcgttgctgg
     5521 tttccctgac gctaacgcca atgatgtgcg gctggatgct aaaagccagc aagccgcgtg
     5581 agcaaaagcg actgcgtggt tttggccgca tgttggtagc cctgcaacaa ggctacggca
     5641 agtcgctgaa atgggtgctc aatcataccc gtctggtggg cgtggtgctg cttggcacca
     5701 ttgcgctgaa tatctcgatc ccgaaaacct tcttcccgga gcaggacacc ggcgtactga
     5761 tgggtgggat acaggcggat cagagtattt cgtttcaggc gatgcgcggc aaattgcagg
     5821 atttcatgaa aattatccgt gacgatccgg cagtggataa tgtcaccggc tttaccggcg
     5881 gttcacgagt taacagtggg atgatgttta tcaccctcaa gccacgcgac gaacgcagcg
     5941 aaacggcgca gcaaattatc gaccgtctgc gcgtgaagct ggcgaaagaa ccgggggcga
     6001 atctgttcct gatggcggta caagatattc gcgttggtgg gcgtcagtcg aacgccagct
     6061 accagtacac gttgttatcc gacgacctgg cggcactgcg cgaatgggag ccgaaaatcc
     6121 gcaaaaaact ggcgacgttg ccggaactgg cggacgtgaa ctccgatcag caggataacg
     6181 gcgcggagat gaatctggtt tacgaccgcg acaccatggc acggctggga atcgacgtac
     6241 aggccgccaa cagtctgtta aataacgcct tcggtcagcg gcaaatctcg accatttacc
     6301 agccgatgaa ccagtataaa gtggtgatgg aagtggatcc gcgctatacc caggacatca
     6361 gtgcgctgga aaaaatgttc gttatcaata acgaaggcaa agcgatcccg ctgtcgtatt
     6421 tcgctaaatg gcaaccggcg aatgccccac tatcggtgaa tcatcaggga ttatcggcgg
     6481 cctcgaccat ctcgttcaac ctgccgaccg gaaaatcgct ctcggacgcc agtgcggcga
     6541 tcgatcgcgc aatgacccag cttggtgtgc cttcgacggt gcgcggcagt tttgccggca
     6601 cggcgcaggt tttccaggag acgatgaact cgcaggtgat cctgattatc gccgccatcg
     6661 ccacggtgta tatcgtgctg ggtatccttt acgagagtta cgtacatccg ctgacgattc
     6721 tctccaccct gccctcggcg ggcgttggag cgctgttggc gctggagctg ttcaatgccc
     6781 cgttcagcct aatcgccctg atagggatca tgctattaat cggcatcgtg aagaaaaacg
     6841 ccattatgat ggtcgatttt gcgcttgaag cccaacggca cggtaacctg acgccgcagg
     6901 aagctatttt ccaggcctgt ctgctgcgtt ttcgcccgat tatgatgacc accctggcgg
     6961 cgctgtttgg cgcgctgccg ctggtattgt cgggcggtga cggctcggag ctgcggcaac
     7021 ccctgaggat caccattgtc ggcggtctgg tgatgagcca gctcctgacg ctgtatacca
     7081 cgccggtggt gtatctcttt ttcgaccgtc tgcggctgcg tttttcgcgt aaacctaaac
     7141 aaacggtaac cgagtaaatg acagatcttc ccgacagcac ccgctggcaa ttatggattg
     7201 tggctttcgg cttctttatg cagtcgctgg acaccaccat cgtaaacacc gcccttccct
     7261 caatggcgca aagcctcggg gaaagtccgt tgcatatgca catggtcatt gtctcttatg
     7321 tgctgaccgt ggcggtgatg ctgcccgcca gcggctggct ggcggacaaa gtcggcgtgc
     7381 gcaatatttt ctttaccgcc atcgtgctgt ttactctcgg ttcactgttt tgcgcgcttt
     7441 ccggcacgct gaacgaactg ttgctggcac gcgcgttaca gggcgttggc ggtgcgatga
     7501 tggtgccggt cggcaggttg acggtgatga aaatcgtacc gcgcgagcaa tatatggcgg
     7561 cgatgacctt tgtcacgtta cccggtcagg tcggtccgct gctcggtccg gcgctcggcg
     7621 gtctgctggt agaatacgcg tcgtggcact ggatcttttt gatcaacatt ccggtgggga
     7681 ttatcggtgc gatcgccaca ttgatgttaa tgccgaacta caccatgcag acgcggcgct
     7741 ttgatctttc cggcttttta ttgctggcgg ttggcatggc ggtattaacc ctggcgctgg
     7801 acggcagtaa aggtacaggt ttatcgccgc tggcgattgc tggcctggtc gcagttggcg
     7861 tggtggcact ggtgctttat ctgctgcacg ccagaaataa caaccgcgcc ctgttcagtc
     7921 tgaaactgtt ccgcactcgt accttttcgc tgggcctggc ggggagcttt gccggacgta
     7981 ttggcagtgg catgttgccc tttatgacac cggttttcct gcaaattggc ctcggtttct
     8041 cgccgtttca tgccggactg atgatgatcc cgatggtgct tggcagcatg ggaatgaagc
     8101 gaattgtggt acaggtggtg aatcgctttg gttatcgtcg ggtactggta gcgaccacgc
     8161 tgggtctgtc gctggtcacc ctgttgttta tgactaccgc tctgttgggc tggtactacg
     8221 ttttgccgtt cgtcctgttt ttacaaggga tggtcaactc gacccgtttc tcctccatga
     8281 acaccctgac gctgaaagat ctcccggaca atctggcgag cagcggaaac agcctgctgt
     8341 cgatgattat gcaattgtcg atgagtatcg gcgtcaccat cgccgggctg ttgctggggc
     8401 tttttggttc gcagcatgtc agcgtcgaca gcggcaccac acaaaccgtc tttatgtaca
     8461 cctggcttag catggcgttt atcatcgccc ttccggcgtt catctttgcc agagtgccga
     8521 acgatacgca tcaaaatgta gctatttcgc ggcgaaaaag gagcgcgcaa tgaagttctg
     8581 gcgacccggt attaccggca aactgtttct ggcgattttc gccacctgca ttgtcttgct
     8641 gatcagtatg cactgggcgg tgcgtatcag ttttgagcgt ggctttattg attacatcaa
     8701 acacggcaat gaacagcgat tacagctgtt aagtgatgcg cttggcgagc agtatgcgca
     8761 gcatggtaac tggcgcttcc tgcgcaacaa cgatcgcttt gtctttcaga tcctgcgttc
     8821 atttgaacac gataattcgg aagataaacc cggcccgggt atgccgccgc acggctggcg
     8881 tacccagttc tgggtggtcg atcaaaacaa caaagtactg gttggtccgc gagcgccgat
     8941 tccacctgac ggtacacggc gacccattct ggtcaacggt gcggaagtgg gcgcggtgat
     9001 tgcctcccct gttgagcggc tgacccgcaa tactgatatc aatttcgata aacaacagcg
     9061 gcaaaccagc tggctgatcg tcgccctggc aacgttactc gcggcgctcg ccacctttct
     9121 gctggcgcgc ggtttgctgg caccggtaaa acgacttatc gatggcacgc acaaactggc
     9181 ggcgggcgat ttcactaccc gcgtaacgcc caccagtgaa gatgaactgg gcaaactggc
     9241 gcaagacttc aaccagctcg ccagcacgct ggagaaaaac cagcaaatgc ggcgcgattt
     9301 tatggccgat atttctcacg aactgcgtac gccattagcg gtgctgcgcg gtgaactgga
     9361 agccattcag gatggcgtgc gcaaattcac gccggagacg gtggcgtctt tacaggcgga
     9421 ggtcggtaca ctgaccaaac tggttgacga tcttcatcag ttgtcgatgt ctgatgaagg
     9481 cgctctcgcc tatcaaaaag caccggtaga tttgatccca ctgctggaag tggcgggcgg
     9541 cgcatttcgc gaacgattcg ccagccgtgg cctgaaactg caattttccc tgccagacag
     9601 tatcaccgta tttggcgatc gcgaccgttt aatgcagtta ttcaataact tactggaaaa
     9661 cagcctgcgc tacactgaca gcggcggcag cctgaaaatc tctgccgagc agcacgacaa
     9721 aacggtgcgc ctgacctttg ccgacagtgc gccaggtgtc agtgacgatc agctacaaaa
     9781 attgtttgaa cgtttttatc gcaccgaagg ttcccgcaac cgcgccagcg gcggttccgg
     9841 gctggggctg gcgatttgcc tgaacattgt tgaagcccat aatggccgca ttattgccgc
     9901 ccattcgcct tttggcgggg taagcattac agtagagtta ccgctggaac gggatttaca
     9961 gagagaagta tgaccgagtt accaatcgac gaaaacacac cgcgtatttt gatcgtggaa
    10021 gatgaaccga agctggggca gttgctcatt gattatctgc gtgctgcgag ctatgcgccg
    10081 acgcttatca gccacggcga tcaggtactg gcgtatgtgc gccagacacc accggatctg
    10141 atcctgttgg atctgatgct ccctggcacc gatggcctga cattatgccg ggaaattcgt
    10201 cgtttttccg atgttccgat cgtgatggtg acggcaaaaa tcgaagagat cgaccgcctg
    10261 ctggggctgg agattggcgc cgatgactac atctgcaagc cgtacagccc acgggaagtg
    10321 gtagcgcgtg tcaaaaccat tttgcgccgt tgtaaaccgc aacgcgaact tcagcaacag
    10381 gatgccgaaa gcccgttgat tatcgacgaa ggtcgttttc aggcttcatg gcgcggtaaa
    10441 atgcttgacc tgacgcctgc ggaatttcgt ctgcttaaaa cgctctctca cgaaccagga
    10501 aaagtgttct cccgcgagca attgctcaat catctttatg acgactaccg cgtagtaacc
    10561 gaccgcacca ttgacagcca cattaaaaat ctgcgccgca agctggaatc tctcgacgcc
    10621 gaacagtcat ttatccgcgc cgtttacggc gttggttacc gctgggaagc cgacgcctgc
    10681 cgcatcgttt agttttagcg acattacttt gttagccgga gatggcgttc agacccgatc
    10741 cggcaacatt attccacgca tggtcagcaa actgacatta tctctccgcc ctggcaggca
    10801 tcagaaaatc gctcatactt taatcagtaa acagcacctt tagatgctgt tttcgataag
    10861 caatttcaat caaggagtca ttatggctgg ttggtttgaa ctcagtaaga gcagtgataa
    10921 tcagttccgg tttgtgctaa aagcgggcaa tggtgagact atcctcacca gcgagctgta
    10981 tacctcaaaa gcctctgcgg aaaagggcat cgcgtcggtg cgtagcaaca gcccgcaaga
    11041 agagcgctat gagaaaaaaa cggcaagcaa cggcaaattc tatttcaatc tgaaagccgc
    11101 taatcatcaa attatcggct ccagccagat gtacgccacc gcgcaatctc gtgaaaccgg
    11161 aattgcctcc gttaaagcca atggcacaag ccagacggtg aaagacaata cgtaattacg
    11221 atgccgggcg tgggttgttg gcgtccggcg ctttaccaga tcccccctac agcgctacaa
    11281 tgcccgccct taaagtgggg gcctccccta accgcttcat caggtgaagc ggatctgacc
    11341 tgtcatcaga acgagagaat tatgtttaaa ccggaactcc tttccccggc gggaacgctg
    11401 aaaaatatgc gttacgcttt cgcttatggc gcagatgctg tttatgcggg ccagccgcgt
    11461 tactccctac gtgtgcgcaa caacgaattc aaccacgaaa atcttcagct cggcatcaat
    11521 gaagcccacg cgctggggaa aaagttttat gtcgtggtca acattgcacc acacaacgcc
    11581 aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg aaatggggcc ggatgcgctg
    11641 attatgtccg atccagggct gattatgctg gtgcgtgagc acttcccaga aatgccgatc
    11701 cacctctcgg tgcaggctaa cgccgtgaac tgggcgacgg tgaaattctg gcagcaaatg
    11761 ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg aagagattga agagatccat
    11821 aatcaggtgc cggatatgga gatcgagatc ttcgttcacg gcgcactgtg catggcctac
    11881 tccggtcgct gcctgctctc tggctatatc aacaagcgcg acccgaacca gggcacctgc
    11941 accaacgcct gccgctggga gtacaacgtc caggaaggga aagaagatga cgttggcaac
    12001 atcgtacaca agtacgagcc gattccggtg caaaatgttg agccgacgct gggtatcggc
    12061 gcgccaaccg acaaagtgtt tatgatcgaa gaagcccagc gtccgggcga gtatatgacc
    12121 gcgtttgaag atgagcacgg cacttacatc atgaactcga aagatctgcg cgccatcgcc
    12181 catgtagaac gcctgaccaa aatgggcgtg cattcgctga aaatcgaagg tcgtaccaaa
    12241 tctttctact attgcgcacg caccgcacag gtttaccgca aagctatcga tgacgccgct
    12301 gcgggaaaac cgttcgatac cagcctgctg gaaactctgg aaggtctggc gcatcgtggc
    12361 tataccgaag gtttcctgcg tcgtcatact cacgacgatt atcagaacta cgaatacggt
    12421 tattcagttt ctgaccgcca gcagtttgtt ggtgagttta ccggtgagcg caagggggac
    12481 ctcgcagcgg tagcggtgaa aaataaattc tccgttggcg acagtcttga gctgatgacg
    12541 ccgcaaggca acattaactt tacccttgag cacatggaaa acgccaaagg cgaagctatg
    12601 ccggtcgcac caggcgatgg ttatactgtg tggctcccgg tcccgcagga tcttgagcta
    12661 aattacgcgc tgctaatgcg taatttctcc ggggaaacca cgcgtaaccc ccacggtaag
    12721 tgattaattt cgattatttt tcccggatgg aaaattctta gaaaccgatc acatacagct
    12781 gcatttatta aggttatcat ccgtttcgct gaaaaacata atccataaaa tgctagctgt
    12841 accaggaacc acctccttag cctgtgtaat ctcccttaca cgggcttatt ttttgtacat
    12901 aagctgtaaa gaaggattga tccctgataa ccgtcttcca ttgaccacat cgatagaatc
    12961 ccccttcata gcacaatgcc tttcacgtaa cggtatcgtg ctcgcccagg ttccggctac
    13021 gcacagccag aacgcgcatg tttgacgctt accaaaaaat attctcactc tccacatttg
    13081 aatgtcagac gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc
    13141 atttttttgc cctgagtaac acagaaatgg gctgcatcat tttttactat attttctgca
    13201 ccagatattc tgcccctggc taaagaagct tcggcttcgg tgtagtattg gttatcgagt
    13261 ttacgctgaa tattactttt atatgcaaga ccaaatttac cgatacttgt ttcatcatta
    13321 tgcacagcac aaccagatat aagaaaaata ctaattaatg atatagcagc tatctttttc
    13381 atctcacctt cccccattaa ataccaacga cactctctag tgtttaaata taataatggc
    13441 atgattatta taattgaata ggattataat aaatgttctg tacaacattt cctacataag
    13501 taggaattac ggacattgag gcccttcagg gtaactccat gggggcttta atatattata
    13561 ttgaagatgc cactgtttag ttgaatatta ggtatatgct cttttttgaa atttataggt
    13621 ggcagccgtt agtattcgct gtccccattg caagctcctg gtggtaacca ctgaatcctc
    13681 catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc accttttgtt
    13741 ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg gcagaatttc
    13801 ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg cgcgaagcaa
    13861 ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg gagaaaggtg
    13921 atgccgcacg atttgtagag gaggcccgga agttgggcgt cgcaacggtg attgccggtg
    13981 gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag ggggatgaca
    14041 tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc agtgtaggga
    14101 ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaatgcc attgcgatag
    14161 atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc ggatttggga
    14221 cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc gtctcttaca
    14281 tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa atccgcggtg
    14341 aaaactttca ctggcaaggt gacgccctgg tcattggtat tggtaacggg cgtcaggccg
    14401 gtggcggtca acaattgtgc ccgaacgcgt taattaacga tggcttgctg caactgcgca
    14461 tttttaccgg cgatgaaatt cttccggctc tcgtatcaac cttaaaatct gacgaagata
    14521 acccgaatat tatcgaaggc gcttcgtcgt ggtttgatat acaagcccca cacgaaatca
    14581 cttttaatct tgatggcgaa ccgttgagtg ggcaaaactt ccatattgaa atacttccgg
    14641 cggcgttgcg ttgtcgatta ccaccagatt gtccattatt gcgttaatca aactattctc
    14701 tcttgaacca gggagaatcc gtaatgccgg atgcggtgta aacaccttat ccggcataca
    14761 gaacaatact acaccgtaat taattttacc cgtgtcttct gcaatgccaa tttatccacc
    14821 tctgacacag catcatcggt aacaataatg tcaatttttt cgattggcag tacctgatta
    14881 aaaccgcgac gattaaattt cgatgaatcg agaacggcaa tcactttatt tgcggcggtg
    14941 accatcgccc cgctaatcga ataaccttca ttaaaggtcg taataccatt aaccgcatca
    15001 ataccatcag caccgacaaa cattaaatcg gcattaatat cctgcaatga acgctcggca
    15061 atggaaccat gcatcgagcg cgttttatgg cggaccgtgc caccacagac gaccagagta
    15121 atgtctttat tttcggaaag ggcaaacgct gccgggagac tgttggtaat caccgtgata
    15181 tttttagcgg tcattaatcc ttcggcgata agcattgtgg tacttccgct gtcgagaata
    15241 acggtcatcc cttcatggat cattttgact gccgcctgcg ctattctgtt ttttggcgcg
    15301 ctggcgagct gaaatcgctc tttaaacccg acttcctggg tctcggtttc actattaccg
    15361 gacattattt tcgccgcacc gccatgaaag cgcgtaacca cgcctttttg ttcgagaaag
    15421 cgcaaatcgg cacggattgt cgcttccgag gcagcaaata ctcccgccag atcctgaaca
    15481 agcacggttc cctgttcatt cactaattgg atgatcttat tccttcgctc gaatgaattc
    15541 atcgtcattt acccatattc aattgtggct agtgtaaacg aagggggacc attggtgaat
    15601 gatctgtttt gcacaatacc gggccagcat cacgctggcc cgcggtttca tggaatgagc
    15661 aacactttgc ccggcatggc attacgagcg atgtcacgca ccgcctgggt gaagctttca
    15721 aagcttccac ggtgagcgat taatggatcc aggcttaact tacgttctgt cagcaaccgg
    15781 ctcgccgttt cccactcctg ccccggccaa gggctggaat agttcatcca actgccgata
    15841 accgtcagct ctttacgcaa tattttgcca aacgttgccg atgttaaatg cagatcctga
    15901 tgcaacgtgc ccaccagcgc cagttgggca tgaggaccgg caatctctac cgccagttcg
    15961 acagtttgcg gtacgccagc cgtctcgagg ataagctgat taaagcgcag ttcgcgtaaa
    16021 acgctctgca tttgcggcgc gctcatttcg ctactgttaa atgtttgcat cgcaccgaaa
    16081 gattttgcca gtgccagttt ttctgagcta atgtcgatcg ccgtcacact ctttgctccc
    16141 agcgcgacag cgcactgaat agccagcagg ccaatggttc cggcaccaat aataataacg
    16201 tttttattct cacaaccttg cgctaaatga aaagcatgca ggccaacggt aatcggctca
    16261 ataaaagccc catcctcaat aggcatatcc gtgggtagag caaagacatt ttttcgctta
    16321 acgacaatat attcagcaaa tccaccatca cgccgcgagc caataaaatc atattttgcg
    16381 cactgggaat aaaacccttt caaacactct ggacaagtaa aacagggtaa taacggcaca
    16441 caggcaaccg catcgccagg gtgtaaatca tcaacaccgg atcccaccgc atcaatatag
    16501 ccgctaaatt catggcctaa cgttattgga taataatgtg caccattttt aaatatcctg
    16561 ggtaaatcgg aaccacataa gcccgagctg gcaattttta cccgcacctc atcctgatgt
    16621 ttaatttcag gaatgacgct ttttgcaacg cgcacgatac catcagtatc attcactact
    16681 gatttcataa aaactcctga ttgttaaggg ggataacccc cccctcaggt aaaaattatt
    16741 ctgcgagaac ggctttctct tgtttagtaa aaccacgcgc tctacgccag gtcatgaaaa
    16801 taccggtca
//
LOCUS       DADLTF010000083        16755 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.083, whole genome shotgun sequence.
ACCESSION   DADLTF010000083 DADLTF010000000
VERSION     DADLTF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.083"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="JN749_002563"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="JN749_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7368935.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="JN749_002564"
     CDS             2219..2767
                     /locus_tag="JN749_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HAY7368936.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="JN749_002565"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="JN749_002565"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HAY7368937.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /locus_tag="JN749_002566"
     CDS             complement(3664..4854)
                     /locus_tag="JN749_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HAY7368938.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN749_002567"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="JN749_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HAY7368939.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN749_002568"
     CDS             complement(6729..8129)
                     /gene="asnS"
                     /locus_tag="JN749_002568"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAY7368940.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN749_002569"
     CDS             complement(8299..9501)
                     /gene="pncB"
                     /locus_tag="JN749_002569"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAY7368941.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9767..12382
                     /gene="pepN"
                     /locus_tag="JN749_002570"
     CDS             9767..12382
                     /gene="pepN"
                     /locus_tag="JN749_002570"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HAY7368942.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISPA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JN749_002571"
     CDS             complement(12589..13356)
                     /gene="ssuB"
                     /locus_tag="JN749_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HAY7368943.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13353..14000)
                     /locus_tag="JN749_002572"
     CDS             complement(13353..14000)
                     /locus_tag="JN749_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7368944.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNI
                     DRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGI
                     GYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JN749_002573"
     CDS             complement(14012..15157)
                     /gene="ssuD"
                     /locus_tag="JN749_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HAY7368945.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15154..16113)
                     /locus_tag="JN749_002574"
     CDS             complement(15154..16113)
                     /locus_tag="JN749_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7368946.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTIGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JN749_002575"
     CDS             complement(16106..16681)
                     /gene="ssuE"
                     /locus_tag="JN749_002575"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HAY7368947.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4184 a         4322 c         4291 g         3958 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agtcgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgttgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg agaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcag ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaattatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agctttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt tttatgacac ctgccactgc cgtcaataag
     6181 ttctgtcaat aaaaatttac ggaactattg atgagagttt ggtgtcttta tgtgtctgca
     6241 ggcatctttc cattcaaact aacgtttcgc taccgtgaaa gtgctacaaa gataaagatt
     6301 tagtttcaaa aagaaaaaat atgtaaccaa aagtaaaaat taaggagctt tgtgaacacc
     6361 gtcatatttc catagaaacg tgatgatatt tacagcaatt tttatctatt tatatgattt
     6421 acttatattt aaattaacta aacggaaatt ttgtttctga tggaaacttt atcgacctgg
     6481 cacaaaatcc tctttcagac atccagaatg ccaaaaaata gtatgaaaat tgtgctatca
     6541 gctaagaaaa aactaatccg caataagaat gcgggttatt ttttgtgcgt aaatgttgca
     6601 atgtaatatt ccgtaacagg atgatcgtta tggcgggggt gcaaatagat tgcttgactt
     6661 aacaaacaga taacttgaca gaaaggataa aaaagagcca gcaggcgctg gctctttgag
     6721 gaagaaaatc agaagctggc gttgcgcgga gtacgtggga acggaatcac atcacgcacg
     6781 ttttgcacgc cagttacgta agcaatcagg cgttcaaaac caagaccgaa acctgaatgc
     6841 ggaacagtac cgtagcgacg cagatcgcga taccaccagt aatcttcttt attcaggccc
     6901 atttccagca tacgctcgtc cagaacgtcc agacgttctt cacgctggga gccaccaatg
     6961 atctcaccga tgcccggagc cagaacgtcc atagccgcaa cggttttacc gtcttcgttg
     7021 aggcgcatat agaacgcttt aatatctttc ggatagtttt taaccactac cggtgcttta
     7081 aagtgttctt ccgccagata acgctcatgc tcagaagaga gatcgacgcc ccagtaaacc
     7141 gggttttcaa acttcctgcc gcagttttcg agaatggtca ctgcgtcggt gtaatccacc
     7201 tgtgcgaaat cggcttcaat gaagcgttcc agacgtgaaa cggcatcttt atctacgcgt
     7261 tcagcgaaga atttcatgtc gtctgcgcgt tcttcgagaa ccgctttgaa gacatatttc
     7321 agcatggctt cagccagacc cgcgatatcg ttcaggttag caaacgccac ttccggctcc
     7381 agcatccaga attccgccag gtgacggctg gtgttggagt tttcagcacg gaaagtcggg
     7441 ccgaaggtat aaattttgga taatgcgcaa gcgtaggttt cgccgttcaa ctggccagat
     7501 acggtcagga aagactcttt accaaagaag tctttgtcga aatccacttt gccctgatcg
     7561 ttacgcggca ggttttccag atccagcgta gaaacgcgga acatttcgcc tgcaccttcg
     7621 gtatcagatg cggtaatcag tggcgttgaa acccagaaga atccctgctc gttaaagaag
     7681 cgatgcagcg cctgcgccag cgtatggcga acgcgcgcga cggcaccaat caggtttgtg
     7741 cgcggacgca ggtgagcgac ttcacgcaga tactcaatgc tgtggcgttt tgccgccatc
     7801 gggtaagtgt ctggatcttc aacccaacca gcaacttcaa ccttgctggc ctgaatttca
     7861 aattgttgcc cctggcccgg cgatgccacg actttacccg tcacaatgac cgagcagccg
     7921 gtggtcagac gcaggacgtc ttcattgtaa ttgggcagag aattattgat gacagcctgt
     7981 acaggatcaa agcaggaacc gtcataaacg gcgaggaagg agatgccagc ttttgaatct
     8041 cggcgggtac gtacccatcc gcgtacggtg acttcgctgt caacggctac gcggccctgg
     8101 agtacgtcgg ctacaggcac aacgctcata atattctctc tgttaatagt cggaaaaaat
     8161 aaacacttgt ccacccgtaa atgggggggg atacctatgt tacctggcat ctgcaatcag
     8221 acaagcagaa ttcgcaaatg cagcgaaaga tttcggaatt aaaggtaaaa aagggagccg
     8281 attagctccc tgatgatatt aactggcttt tttaatatgc ggaaggtcga acgctttgcg
     8341 cagcgcccga acaaacgctt tatcatggca gatagttttg ccagggctgt cagaaagttt
     8401 cgccaccggt ttaccgttac actctaccaa cttaatcaca atattcaggg gttttacctg
     8461 ggggatatcg caggtcaggc gagtcccaat accaaaactt aattgcacgc gggaagagaa
     8521 gtggcggtat agctcaaccg ctttgcgtaa atccagattg tcagagaaaa ccagcgtttt
     8581 actctgtgga tcaattccca gcttttcata atgtgcaatg gctttttcac cccattcaac
     8641 cgggtcgcca gagtcatgac gcaggccctg ataccgacta gcgaactcga caccgaaatc
     8701 acgcaggaaa gcatccatag tgatgcagtc ggttaatgca atgccaagtt ggtcgggata
     8761 ctcttccagc caggcagcaa gtgcagctcg ctggctgttg gctagatccg ggctgatttg
     8821 ctgatgtgcc tggaaccatt cgtgtgcctg tgttcccatc ggcgtgaggg aaagccgacg
     8881 cgccagatcg tagttgctgg tgcccacgaa ccaggattcc tgttgcagac gcttaacgat
     8941 ggtttcttgt acttcgcgag aaaaacggcg acgggtgcca aaatccatca gatggaagcg
     9001 cgacatatca agaccggcgg ttaacgccga gaagtcgact aatttgcttt ccagcgtgtc
     9061 gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga tgtaccattt cactgataac
     9121 cgccagcaaa ggaacttccc agaggatgac ttcacgccac ggaccgctta agcgaatatc
     9181 cagcttgcca ttatcgttgg acacggtgac ttgttccggg ttaaagcgga actcgcgtaa
     9241 ccagttaaga tagtcggcct taaagaaagg cagggcagag agccactgat attcatcatc
     9301 ctgcaggcgc aggtgctgca tcgcctgaat ctgttcacga atagcatcgg cataaatacc
     9361 cagcagatcg tcacctcggc aacgaaactc cgccgcgaca tgcacatcgt aatagtgatg
     9421 aaacacggct tgctgcatat gcaacttata agcatctgta tccagcaacg agtgcagaac
     9481 aggagaagcg aattgtgtca taggtgcgca gtagcgtcct cgtacaggag cgtttagtac
     9541 aataaacatc ttcaggaact gctggagtat accttgtttc gcaatttatt gaaccccgat
     9601 cacaccatat gccacctttc tggtcgatgg cattacgcgg tgtatgttat aaaaatgtag
     9661 caataaaggc gtttgtacct gaaaagatga agattctgca tagcgcgatt tacgcaacag
     9721 gaatagactg aacaccagac tctataaaag atgctaaagg ttatttatga ctcaacagcc
     9781 acaagccaaa taccgtcacg attatcgtgc gccggattac cagattactg atattgactt
     9841 gacctttgac ctcgacgcgc aaaagacggt cgttaccgcg gtcagccagg ctgtccgtca
     9901 tggtgcatca gatgctccgc ttcgtctcaa cggcgaagac ctcaaactgg tttctgttca
     9961 tattaatgat gagccgtgga ccgcctggaa agaagaagaa gagggcgcac tggtcatcag
    10021 taatttgccg gagcgtttta cgcttaagat cattaatgaa ataagcccgg cggcgaatac
    10081 ggcgctggaa gggctttatc agtcaggcga tgcgctttgc acccagtgtg aagccgaagg
    10141 tttccgccat attacgtatt atctcgaccg cccggacgtg ctggcgcgtt ttaccaccaa
    10201 aattattgcc gataaaatca aatatccctt cctgctttcc aatggtaacc gcgttgcgca
    10261 aggcgaactc gaaaacggac gccattgggt acagtggcag gacccgttcc cgaaaccgtg
    10321 ctacctgttt gcgctggtgg caggcgactt tgatgtactg cgcgatacct ttaccacgcg
    10381 ttctggtcgc gaagtagcac tggagctgta cgtcgatcgc ggcaaccttg atcgcgcgcc
    10441 gtgggcgatg acctcgctga aaaactccat gaaatgggat gaagaacgct ttggcctgga
    10501 gtatgacctc gacatctata tgatcgtcgc ggtggatttc ttcaatatgg gcgcaatgga
    10561 gaataagggt ctgaatatct ttaactccaa atatgtgctg gcccgcaccg acaccgccac
    10621 cgacaaagat tacctcgata ttgaacgcgt tatcggccat gaatatttcc ataactggac
    10681 cggtaaccga gtgacctgcc gcgactggtt ccagctcagc ctgaaagaag gtttaaccgt
    10741 cttccgcgat caggaattca gctctgacct tggttcccgc gcagttaacc gcatcaataa
    10801 tgtgcgcacc atgcgcggat tgcagtttgc ggaagacgcc agcccgatgg cgcacccaat
    10861 ccgcccggac atggtcattg agatgaacaa cttctacacc ctgaccgttt acgagaaggg
    10921 cgcggaagtg atccgtatga tccacaccct gctgggcgaa gaaaacttcc agaaagggat
    10981 gcaactctat ttcgagcgtc atgatggtag cgcagcgacc tgtgacgact ttgtgcaggc
    11041 gatggaagat gcgtcgaatg ttgatctctc ccatttccgc cgttggtaca gccagtccgg
    11101 tacgccggtt gtgaccgtca aagacgacta caatccggaa actgagcagt acaccctgac
    11161 catcagccag cgcacgccag ccacgccgga tcaggcagaa aaacagccgc tgcatattcc
    11221 gtttgccatc gaattgtatg acaacgaagg caaagtgatc ccgttgcaga aaggcggtca
    11281 tccggtgaat tccgtgctga acgtcaccca ggcggaacag acctttgttt ttgataatgt
    11341 ctacttccag ccggtgcctg cgctgctgtg cgaattctct gcgccagtga aactggaata
    11401 taagtggagc gatcagcaac tgaccttcct gatgcgtcat gcgcgtaatg atttctcccg
    11461 ttgggatgcg gcgcaaagtt tgctggcaac ctacatcaag ctgaacgtcg cccgtcatca
    11521 gcaagggcag ccgctgtctc tgccggtaca tgtggctgac gctttccgcg cggtgctgct
    11581 cgatgagaag attgatccgg cgctggcggc agaaatcctg acgctgcctt ctgtcaatga
    11641 aatggctgaa ctgttcgata tcatagaccc gattgctatt gccgaagtac gcgaagcact
    11701 cactcgtact ctggcgactg aactggcgga tgagctgctg gctatttaca acgcgaatta
    11761 ccagagcgag taccgtgttg agcatgaaga tattgcaaaa cgcactctgc gtaatgcctg
    11821 cctgcgcttc cttgcttttg gtgaaacgca tctggctgac gtgctggtga gcaagcaata
    11881 ccatgaagca aacaatatga ccgatgcgct ggcagcgctt tctgcggcgg ttgctgcaca
    11941 gctgccttgc cgtgacgcgc tgatgcagga gtacgacgat aagtggcatc aggacggtct
    12001 ggtgatggat aaatggttta tcctgcaagc caccagcccg gcggcgaatg tgctggagac
    12061 ggtgcgtggt ctgttgcagc atcgctcatt taccatgagc aacccgaacc gtattcgttc
    12121 gttgattggt gcgtttgcgg gcagcaaccc ggcagcgttc catgccgaag atggcagcgg
    12181 ttaccagttc ctggtggaaa tgcttaccga cctcaacagc cgtaacccgc aggtagcttc
    12241 acgtctgatt gaaccgctga ttcgcctgaa acgttatgat gccaaacgtc aggagaaaat
    12301 gcgcgcggcg ctggaacagt tgaaagggct ggaaaatctc tctggcgatc tttacgagaa
    12361 gataactaaa gcactggctt gatacttttg agccaaaatg cctgatgcga cgcttgacgc
    12421 gtcttatcag gcctacaact ccgttgaaat tgctggtttt gtaggctgga taaggcgttc
    12481 acgccgcatc cggcagccgt gcccaactgc ctgatgcgat gcttgacgtg tcttatcagg
    12541 cctacaagtc cgttgaaaat gctggttttg tgcgccacat ccggcatatt aaccatgacg
    12601 ccgaatcagt tgttcagaat gcccacgccg cattacccgt tgcaatactt ccgcttccag
    12661 ctccgctaat cgcaccgacc ctaatcgacg cggacggggg atatccactg tcaaatccag
    12721 actaattttt ccctcttcaa ttaacaacac ccggtcagcc attgctaccg cttcgctcac
    12781 atcatgcgtc accaacagta cggtaaagcc gtgctgctgc caaagtgaca caatcaaatc
    12841 ctgcatctcg agtcgcgtta aggcgtccag tgccccgagc ggttcatcaa gcaacaataa
    12901 acccggtcga tgaatcaacg ctcttgccag cgccacacgc tgtttctgcc cgccagaaag
    12961 tgcggcaggc cattccccgg cgcgattctc cagtcctacc gcagccaacg cccgacgcgc
    13021 ggcatcgcgc cactgacctt taaggcctaa cccaacgtta tcaatcaccg atttccacgg
    13081 cagcagacgc gcatcctgaa acatcattcg cgtatcatcc tgaatttcag ccagcggtgt
    13141 ggtgcccgct aacacatcgc ctgcggttgg cgtttccaga ccggccagca ggcgcagcag
    13201 ggtactttta ccaccaccgc tgcggcccac caccgccaca aactgacctg ccggaatatg
    13261 taaatccagt tggttcagga cgatattttc cgcgtaatgt ttgcttactg cattgagcaa
    13321 caatggtgtg ccctggttca gacgagcagt attcatatcg tggcctcctt caaatgataa
    13381 gccgggttcc agctcaacca gaggcgctct aacagctgcg cgctgacgtc agcaagtttg
    13441 ccgagcaggg cgtaaagaat aatagcgact accaccacgt ctgtttgcaa aaactctcgc
    13501 gcattcatcg ccagataacc aatgccggaa ttggcagaaa tggtttcggc aacaatcagc
    13561 gtcagccaca tcaggcccaa cgcaaaacgc acgccgacca taattgaggg cagggcatcg
    13621 ggcaggatca catggataaa cagcggtatg ccggataatc catagctacg cgccatctcc
    13681 accagcccgc gatcgatatt acggatgcca tgccaggtgt tgatataaat ggggaacagc
    13741 gtaccgagcg ccaccagaaa gattttcgcg gattcatcaa tgccaaacca caaaatcacc
    13801 agcgggatca gcgccagatg ctgccacagt tcgccgctgg cggagagcgt ccagaacgcc
    13861 gtcaccactc cttccggtga aggcaaaata cgcgtcgaca gccagccaac cgaggaggcc
    13921 agttgccaca ccgccacgat gcccaccggt aaaaaccagg gggcaacgcg caataaccac
    13981 ttcttcactg gcgttgccat catcgtgttc cttagctttg cgcgacttta cgggggataa
    14041 aatcattcgc caccgcttcg ccttgcggat tcagcggctg cggctgggga atttccggaa
    14101 tggcgacatc cagatgtggg aacagcaact cgccaacccg atacgcttct tccagatgcg
    14161 ggtagcccga aagcacaaag ctgtcgatgc caagcgcggc gtattcgttg attcgcgcgg
    14221 cgaccgtagg accatcgccc accagcgccg ttccggcacc gccgcgcact aagccaacgc
    14281 ccgcccataa attggggctg atctccagat tgtcgcgctt gccgttatgt agcgccgcca
    14341 ttcgctgttg tcctacggaa tccgtccggg cgaatgcggc ttgtgctttg gcgatagtct
    14401 catcatcaag atgcgagatt aaccgctcgg cggcctgcca cgcttcgtcg ttagtttcac
    14461 gaacaatcac atgcagacga ataccgaaac gaattttgcg cccatacgcg gcagctttcg
    14521 cccgcacttg ttcgattttc tctttaacaa gttccggcgg ttcgccccag gtgaggtaga
    14581 gatcaacttg ttctgccgcc agctcctggg cgacatctga cgatccgcca aagtaaagcg
    14641 gaggataagg ctgttgaatc ggcgggaaga gcagttttgc tccgcgcaca tgaatatgtt
    14701 taccgttgaa attgacggtt tcccccagca ataagcgccg ccagacctgg gtaaattccg
    14761 ccgaggcttc gtagtgctcg ctatgatcaa ggaacacgcc gtcgcctgcc agctcttgtg
    14821 gatcgctgcc tgtgaccaga ttaaacaacg cacgaccatt tgagagacgg tcaagcgtgg
    14881 cggcctggcg ggcggcaaca gtaggtgagg ttacgctggg acgcagggcg acaagaaact
    14941 tcagccgctg cgtcaccggg atcatcgacg ctgcaaccag ccacgcatct tcacacgatc
    15001 gccccgttgg gatcagcaca ccggtatagc ccagacgatc cgccgcttgc gcaatttgtt
    15061 gcagataacc gtgatcaacc gggcgtgaac cttcttccgt tcccagatag tgcccgtcac
    15121 cgtgggtcgg taaaaaccag aacatattca gactcataat tgttttcctt ccagttgagt
    15181 gggctgccag atgcgctggc gaatatcgac ttttttcggc accagacggt tttcataaaa
    15241 cagatctgcc gtttgctgct gtaaggcggc aacctcggcg ttaaccggtt tgatggtggt
    15301 aggagggcga tgatctaagt aagaggcaat caccggtgcc ggtaagccaa tcgtttttgc
    15361 cagtaaagcg atgctttgtt cgcgctggct gcgggttaac gcatcggcct cactaaaggt
    15421 tgccagtacg ccctgaataa aagcgccgtt tttttctgca tagggacgag ctgccagata
    15481 aaacgatcca gtttgattga gatcggtgcc gtctttcagc acccgcacgc cgccctgtaa
    15541 taacgcagcg gagtagtagg gatcccagat agcccaggcg tcaacgttac cttgctggaa
    15601 cgcggcgcgg gcaccggcag gcgtcaggta agtgggctgg atatcggtga atttaagtcc
    15661 ggcctgacgc agtgcacgca gtaaaaggtt gtgtgaactg gaacctttct gaaaggcaac
    15721 tttgtgacct ttaagatcgg ctacggtttt gatcgggctg ttttctgcca ccagaatcac
    15781 ttcggctttg ggcttcggtg gctcgacgcc cacatatacc aaatcagccc cggcagcctg
    15841 ggcaaagatt ggcggaatat ccccggtact gccgagatca atactgccaa cgtttaacgc
    15901 ttccaacatt tgcggacccg cggggaactc cacccaggag atttttgatt gcggatagcg
    15961 tttttccagt aactggtggc tttttgccag caccatacca atactgcctt tctgatagcc
    16021 tatacgtaac gcttcaggcg aggattctgc agcaaccgca aaagtagaga cgctaagcaa
    16081 tcccgccagc gccagtttaa tgatgttacg catgggcatt acctcgcaga gacagaaggt
    16141 caggaacctg aacatcgcgg cggtgcaatg cctgccagaa agtttctagc gcggtatcaa
    16201 gatggggttg cagatttggc gtgaactggg gtctgtgatg gtaatcaatt aattgtgagt
    16261 catcggcaaa cacgccgtgc aggatctcct gagctttcag tgcgcttaaa actggtttaa
    16321 gggcataatc gaccgccagc agatgggcca cggtaccgcc cgtcgccagc ggtagcacca
    16381 ctttgccttg caaagcgcgc tctggcagca ggtcgagcag ggttttcagc gcgccggaat
    16441 aggcggcttt atacacaggc gtggcgacaa tcagcccatc agcctgttgc agctgttcgg
    16501 tgaaggtctt gagtgccgga ctatcgaaac gagcataaag cagatcttcc ggggcgaagt
    16561 tttgcagatt ccagtgataa acctctacat ctaggccatt tagtttttcc cgcgcatatt
    16621 ccagcaagga gctggagcga gaaggaaagc gaggactacc cgccagggtg atgacacgca
    16681 tactctctcc ttataaccaa ttgttctttt ttttgttaac attgataaca attcggtcag
    16741 tctgtcggag agaca
//
LOCUS       DADLTF010000084        16683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.084, whole genome shotgun sequence.
ACCESSION   DADLTF010000084 DADLTF010000000
VERSION     DADLTF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.084"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..2081
                     /gene="pbpC"
                     /locus_tag="JN749_002576"
                     /pseudo
     CDS             1..2081
                     /gene="pbpC"
                     /locus_tag="JN749_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708358.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2231..2662
                     /gene="ndk"
                     /locus_tag="JN749_002577"
     CDS             2231..2662
                     /gene="ndk"
                     /locus_tag="JN749_002577"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAY7368948.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="JN749_002578"
     CDS             2812..3966
                     /gene="trmG/rlmN"
                     /locus_tag="JN749_002578"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HAY7368949.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4251..5264
                     /gene="rodZ"
                     /locus_tag="JN749_002579"
     CDS             4251..5264
                     /gene="rodZ"
                     /locus_tag="JN749_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HAY7368950.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5291..6409
                     /gene="ispG"
                     /locus_tag="JN749_002580"
                     /gene_synonym="gcpE"
     CDS             5291..6409
                     /gene="ispG"
                     /locus_tag="JN749_002580"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HAY7368951.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6520..7794
                     /gene="hisS"
                     /locus_tag="JN749_002581"
     CDS             6520..7794
                     /gene="hisS"
                     /locus_tag="JN749_002581"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAY7368952.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7812..8432
                     /locus_tag="JN749_002582"
     CDS             7812..8432
                     /locus_tag="JN749_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HAY7368953.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8443..9621
                     /gene="bamB"
                     /locus_tag="JN749_002583"
     CDS             8443..9621
                     /gene="bamB"
                     /locus_tag="JN749_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HAY7368954.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9739..11211
                     /gene="der"
                     /locus_tag="JN749_002584"
     CDS             9739..11211
                     /gene="der"
                     /locus_tag="JN749_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAY7368955.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11281..11496
                     /locus_tag="JN749_002585"
     CDS             11281..11496
                     /locus_tag="JN749_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368956.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="JN749_002586"
     CDS             complement(11493..12863)
                     /gene="xseA"
                     /locus_tag="JN749_002586"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAY7368957.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13025..14491
                     /gene="guaB"
                     /locus_tag="JN749_002587"
     CDS             13025..14491
                     /gene="guaB"
                     /locus_tag="JN749_002587"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAY7368958.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14560..16137
                     /gene="guaA"
                     /locus_tag="JN749_002588"
     CDS             14560..16137
                     /gene="guaA"
                     /locus_tag="JN749_002588"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAY7368959.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16232..>16683)
                     /locus_tag="JN749_002589"
     CDS             complement(16232..>16683)
                     /locus_tag="JN749_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368960.1"
                     /translation="EAEQQGKNEEQRQHDEWVAERNREIQQEKQRRANAQAAANKRAA
                     TAAANKKARQDKLDAEATADKKRDQSYEDELRSLEIQKQKLALAKEEARVKRENEFID
                     QELKHKAAQTDVVQSEADANRNMTEGGRDLMKSVGKAEENKSDSWFN"
BASE COUNT         3844 a         4170 c         4671 g         3998 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc gttacgttgc aggggatcgg tgcggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgcccag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaactg gaaagggcgg ttgccaccgc
      661 gcagctcact ggcgatgatc gtggttaatc ataccgatat gcgtgttcgc ggctgggtgg
      721 gatcggttga tctcaacgat gattcacgct ttggtcatgt tgatatggtc aatgcgatcc
      781 gatcgccagg atcggtgctc aaaccgtttg tttatggtct ggcgctggat gaaggcttga
      841 tccacccggc atcactgctg caagacgtcc cccggcgcac cggtgattat cgaccaggta
      901 actttgatag cggttttcat ggcccgatca acatgagcga ggcgctggtg cgctcgctga
      961 acttacctgc tgtgcaggtg ctggaagcct atggaccgaa acggtttgcg gcaaagttac
     1021 gcaatgttgg attgccgtta tatttgccca acggcgctgc gccgaatctt tcactcattc
     1081 tcggcggcgc tggtgcaaaa ctggaagata tggcggcagc gtataccgcg tttgctcgcc
     1141 acggcaaggc aggcaaattg cgcttacagc ctgacgatcc gctgcttgaa cggcctttaa
     1201 tgtcgtcggg ggcggcgtgg atcattcgcc ggattatggc tgatgaagcg caacccttgc
     1261 cggatagtgc cttgccgcgc gtcgccccac tggcatggaa aacgggcacc agctatggct
     1321 atcgtgacgc ctgggcgatt ggggttaacg ctcgctatgt cattgggatc tggactggca
     1381 gacctgacgg cacgcccgtt gttggtcagt ttggctttgc cagtgccgta ccattgttga
     1441 atcaggtcaa taatatctta ctgtcgcgca gtgcgaatct gccggaagac ccgcgcccga
     1501 actcagtcac tcgtggcgtt atctgctggc cgggcggaca gtctttgccg gaaggtgacg
     1561 gtaactgtcg ccgccgcctg gcaacctggc tgctggatgg cagccagccg ccaacgctgt
     1621 tactgtcaga gcaggaaggg attaatggca ttcgcttccc catctggctg gatgacaaag
     1681 ggcagcgcgt cgccgctgac tgcccgcagg cgaagcaaga aatggtaaat gtctggccgc
     1741 taccgctgga accctggctg cccgcatcag aacgtcgcgc tgtgcgctta ccaccggcct
     1801 cgacaatttg cccgccatac ggtcacgatg cccagctccc tctgcagctg acaggcgttc
     1861 gcgatggcgc gattattaaa cgtttaccgg gcgcagcgga agcgactttg ccgttgcaat
     1921 caagtggagg ggcaggtgaa cgctggtggt ttctgaatgg cgaaccgtta actgaacgcg
     1981 ggcgcaacgt gaccctgcat ttgacggata aaggtgatta tcaattgctg gtgatggatg
     2041 atgtggggca aatcgcgaca gtgaaatttg tcatgcaata gtctgttttg tccctgattg
     2101 ttgctaaaac tcattttatt ttaaaaaaaa tgttaccttc atcaatagtc aacggccctg
     2161 ttgctcatta taatccgcgc catctcgtac gctggtacag acaacaacag aacaatttac
     2221 agaggtaaaa atggctattg aacgtacttt ttccatcatc aaaccgaacg cggtagcaaa
     2281 aaacgtcatt ggtaatatct ttgcgcgctt tgaagctgca gggttcaaaa ttgtcggcac
     2341 caaaatgctg cacctgaccg ttgaacaggc acgtggcttt tatgctgaac acgatggaaa
     2401 accgttcttt gatggtctgg ttgaattcat gacctctggc ccgatcgtgg tttccgtgct
     2461 ggaaggtgaa aacgccgttc agcgtcaccg cgatctgctg ggcgcgacca atccggcaaa
     2521 cgcactggct ggtactctgc gcgctgatta cgctgacagc ctgaccgaaa acggtaccca
     2581 cggttctgat tccgtcgaat ctgccgctcg cgaaatcgct tatttcttcg gcgaaggcga
     2641 agtgtgcccg cgcacccgtt aataatttcg taaatgccgc gtgcaaacgt ggcatccgtg
     2701 cgccagaatt tgtacaatgc agcgcccccg gacgagcagc cgctcaccgg ggcgtttctt
     2761 ttttcaaccc tccaggggcc ataacgtgta ataacgaggc cggaatagca tatgtctgaa
     2821 caattagtca cacctgaaaa cgtcaccacg aaagatggaa aaatcaacct gctggatctc
     2881 aaccgtcagc agatgcggga gttttttaaa gatttaggtg aaaaaacctt ccgcgccgat
     2941 caggtgatga agtggatgta tcactattgc tgcgacaact ttgatgagat gaccgacatc
     3001 aacaaagtgt tgcgcggcaa actgaaagag gtggcggaaa tccgcgcacc ggaagtggtt
     3061 gaagaacagc gttcatccga cggcaccatt aaatgggcga tcgccgttgg cgatcagcgc
     3121 gtcgaaacgg tgtatatccc ggaagacgac cgtgccacgc tctgcgtctc ttcgcaggtg
     3181 gggtgtgcgc tggagtgtaa attctgttcc accgcccagc agggctttaa ccgcaacctg
     3241 cgggtatcgg aaattattgg ccaggtgtgg cgtgcggcga aaatcgtcgg cgcagcaaaa
     3301 gtcaccggtc agcgcccaat cactaacgtc gtgatgatgg gcatgggcga gccgctgctc
     3361 aacctgaaca acgtcgttcc ggcgatggaa atcatgcttg atgatttcgg ttttggcctg
     3421 tctaaacgtc gcgtgacgct ttccacttcc ggtgtagttc cggcgctgga taaactgggc
     3481 gatatgatcg acgttgcact ggcaatttcc ctgcacgcgc cgaacgacga aattcgtgac
     3541 gaaattgtgc cgatcaacaa aaagtacaat atcgaaacgt tccttgctgc ggtgcgccgt
     3601 tatctggaga aatccaacgc caatcagggc cgagtcacta ttgaatacgt gatgcttgac
     3661 cacgttaacg acggcactga acacgcgcac caactggcgg aactgctgaa agatacgccg
     3721 tgtaagatca acctgatccc gtggaacccg ttcccgggcg cgccgtatgg acgcagttcg
     3781 aacagccgta tcgaccgttt ctcaaaagtg ctgatgagct acggtttcac cactattgtg
     3841 cgtaaaacgc gtggtgatga tatcgatgct gcctgtggtc agttggcggg cgatgttatc
     3901 gaccgtacga aacgtactct gcgtaaacgg atgcagggtg aagccatcga tattaaagcg
     3961 gtctgatagc cattacgcca cggttacttt atgtaccgtg gcgtaatctt ttatgaatga
     4021 agtattaagt tgcaattgtc tggtcaatat taacggtgcg tttttgctga ctttaaggca
     4081 gtatgtaacg atgcaacagt aagttagcct cagtgaatgt gggctttgtc acgagcacac
     4141 agacggtctt atactgtatg ataacggtta acttaacgga tgtttcgcgg tgtgggtgag
     4201 catgatggtt caccggcatc tcaattctca tttaaacgta cctgcagcga atgaatactg
     4261 aagccacgca cgaccaaaat gaagcactta ctaccggcgc tcgcctgcgt aatgctcgcg
     4321 aacaactagg acttagtcag caggccgttg ccgagcgact ttgcctgaag gtttccacgg
     4381 tacgcgacat tgaagaagat aaggcacccg ccgatcttgc ttcaacattc ctgcgcggat
     4441 atatccgctc ttatgcgcgt ctggtacata ttccagaaga agaactgctg ccagggctgg
     4501 aaaagcaggc tccacttcgg gctgcaaaag ttgcgccaat gcagagtttt tccctcggta
     4561 aacgccgcaa aaaacgcgac ggctggctga tgaccttcac ctggctggtg ttgtttgtgg
     4621 ttatcggcct gagcggtgcc tggtggtggc aagaccacaa agctcagcag gaagagatca
     4681 ccactatggc cgatcaatct tcggcggaac tgagcagtaa tagcgagcag gggcagagtg
     4741 ttccgttaaa tacgtcgaca actacagacc cggctacaac gtccacgccg ccagcgtctg
     4801 tggatactac cgcaaccaac acgcaaacac ctgccgtaac tgcgccagca ccagctgttg
     4861 atccgcaaca gaatgcggtt gtttcgcctt cgcaggcaaa tgttgatacc gccgcgaccc
     4921 cggtaccaac ggcagcaaca acgccagatg gtgctgcgcc gttgccaacc gatcaggctg
     4981 gcgtgaccac gccggcggct gatccgaatg cgctggtgat gaactttact gccgattgct
     5041 ggctggaggt cactgatgct accggtaaaa aattgtttag cgggatgcag cgtaaagacg
     5101 gtaatttgaa tttaaccggc caggcaccgt ataaactgaa aattggtgcg ccagccgcag
     5161 tacagatcca gtatcaaggg aaacctgtcg atctgagtcg ttttatcaga actaaccagg
     5221 ttgcgcgtct gaccctcaat gccgaacaat caccggcgca gtaacagacg ggtaacgcgg
     5281 gagatttttc atgcataacc aggctccaat tcaacgtaga aaatcaacac gtatttacgt
     5341 tgggaatgtg ccgattggcg atggtgctcc catcgccgta cagtccatga ccaatacgcg
     5401 tacgacagat gtcgaagcaa cggtcaatca aatcaaggcg ctggaacgcg ttggcgctga
     5461 tatcgtccgt gtctccgtac cgacgatgga cgcggcagaa gcgttcaaac tcatcaaaca
     5521 gcgggttaac gtgccgctgg tggctgacat ccacttcgac tatcgcattg cgctgaaagt
     5581 agcggaatac ggcgtcgatt gtctgcgtat taaccctggt aatatcggta atgaagagcg
     5641 tattcgcatg gtggttgact gtgcgcgcga taaaaacatt ccgatccgta ttggcgttaa
     5701 cgccggatcg ctggaaaaag atctgcaaga aaagtatggc gaaccgacgc cgcaggcgtt
     5761 gctggaatcc gccatgcgtc atgttgatca tctcgatcgc ctgaacttcg atcagttcaa
     5821 agtcagcgtg aaagcgtctg acgtcttcct cgctgttgag tcttatcgtt tgctggcaaa
     5881 acagatcgat cagccgctgc atctggggat caccgaagcc ggtggtgcac gcagcggggc
     5941 agtaaaatcc gccattggtt taggtctgct gctgtcggaa ggcatcggcg acacgctgcg
     6001 cgtatcgctg gcggccgatc cggtcgaaga gatcaaagtc ggtttcgata ttttgaaatc
     6061 gctgcgtatc cgttcgcgag ggattaactt catcgcctgc ccgacctgtt cgcgtcagga
     6121 atttgatgtt atcggtacgg ttaacgcgct ggagcaacgc ctggaagata tcatcactcc
     6181 gatggacgtt tcgattatcg gctgcgtggt gaatggccca ggtgaggcgc tggtttctac
     6241 actcggcgtc accggcggca acaagaaaag cggcctctat gaagatggcg tgcgcaaaga
     6301 ccgtctggac aacaacgata tgatcgacca gctggaagca cgcattcgtg cgaaagccag
     6361 tcagctggac gaagcgcgtc gaattgacgt tcagcaggtt gaaaaataat aacgtgatgg
     6421 gaagcgcctc gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag
     6481 cgttgagggt tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc
     6541 attcgcggca tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc
     6601 acactgaaaa acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag
     6661 cagaccccgc tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg
     6721 tacacctttg aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc
     6781 tgtgtacgcg ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat
     6841 atcgggccga tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag
     6901 ttgggctgcg aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc
     6961 actgcccgct ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct
     7021 atcggttcgc tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag
     7081 cataaagaaa agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg
     7141 ctggattcaa aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat
     7201 tatctggacg aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg
     7261 gggatcgctt acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc
     7321 gttttcgagt gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt
     7381 tatgacggtc ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg
     7441 ggcctcgaac gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct
     7501 gttgtcgata tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta
     7561 gctgagcgtc tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc
     7621 aactttaaga aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg
     7681 ggtgagtctg aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa
     7741 acggcagttg cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg
     7801 agaaggacag cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct
     7861 tttttgctga aaatggcaaa gcactggctg ttggggtgat tttgggcgtt ggcgcactga
     7921 ttggctggcg ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct
     7981 atcaaaatgc ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa
     8041 aatttgctgc tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc
     8101 aatttgttga caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag
     8161 acacgagcga tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac
     8221 agctcaagca ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg
     8281 ctgccattgt tgccgacctg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg
     8341 cgcgtagtgc atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga
     8401 tgcagatgaa aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta
     8461 ctgctgccag gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa
     8521 gaagatgtgg taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg
     8581 gcgtggagca cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca
     8641 ctggcggaca acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg
     8701 gatgatggca aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa
     8761 gagcctgcat tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc
     8821 gaaaaggcgc aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaca
     8881 gtcgcgggtg aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc
     8941 agtaacggtc agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac
     9001 ctcgatatgc cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg
     9061 gctgtcgtgg gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg
     9121 atttggcagc agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat
     9181 gttgacacga ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac
     9241 ctgacggcgc ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg
     9301 aatgatttca tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg
     9361 gcgttgacca ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg
     9421 ctgacttctc cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg
     9481 cactggatta acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt
     9541 ttccagactg aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc
     9601 gtgtactcta ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag
     9661 gggccgtttt cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat
     9721 aaaaatgagg ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga
     9781 aaatccacgt tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg
     9841 ggtctgactc gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt
     9901 attgataccg gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag
     9961 tcgctgctgg cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc
    10021 ctgatgccgg cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc
    10081 ctggtggcaa acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg
    10141 cttggtttag gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg
    10201 ctggagcatg tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa
    10261 gacgctgaat actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac
    10321 gacttcgacc cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt
    10381 aagtctacac tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct
    10441 ggcacgacgc gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc
    10501 attgacaccg ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc
    10561 gtaatcaaaa cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg
    10621 cgcgaaggta tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc
    10681 tcacttgtca ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg
    10741 aaagaaacgc tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct
    10801 gccttgcacg gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc
    10861 tccacccgtc gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat
    10921 caccaaccgc cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt
    10981 tataacccgc cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac
    11041 aagcgctact tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt
    11101 attcagttca aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc
    11161 cagatgcgta aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta
    11221 agtattttat ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat
    11281 atggaactgc attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt
    11341 tgcccatcct gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg
    11401 ttgcaggtgt taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg
    11461 atatcgaaaa aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt
    11521 accggctgga tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc
    11581 atttcacccg ctttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta
    11641 acgctgtatc cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca
    11701 ttaccgaaac gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat
    11761 tccagttgct gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc
    11821 agccgctgtg ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca
    11881 aagctcatcc gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc
    11941 ggatgctgct gctgcaagcg atgatgaatc tgcgtaaagc gacgcgtacg gttggcgaga
    12001 taataatcca tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct
    12061 tgctgattac ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca
    12121 acaaaatcgg caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg
    12181 ctggcaaaaa tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa
    12241 ccgccgccgc gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg
    12301 gcgcgaacaa tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc
    12361 ggtagagaag gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt
    12421 tttgaggtga tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga
    12481 tcgaacaaac cttcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc
    12541 ccttcaccgg ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag
    12601 agcgtaatat tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg
    12661 cggttgctgt tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag
    12721 taccagtgac cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt
    12781 cccatctcat gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt
    12841 gcaggggatt gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca
    12901 gtcgatagta acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca
    12961 atcggttacg ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt
    13021 gcccatgcta cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc
    13081 tcattctacc gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg
    13141 tctgaatatc cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat
    13201 tgctctggct caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca
    13261 ggcagaagaa gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac
    13321 tgttctgcca accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc
    13381 gggctatccg gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt
    13441 gcgttttgtt accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct
    13501 ggtcaccgtg cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg
    13561 cgttgaaaaa gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa
    13621 agacttccag aaagcggaac gtaaaccgaa cgcctgtaaa gacgagcaag gccgtctgcg
    13681 tgttggtgct gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc
    13741 cgctggcgtt gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca
    13801 gcgtatccgt gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc
    13861 aacagctgca ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat
    13921 cggccctggt tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac
    13981 tgccgttgct gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg
    14041 tggtattcgc ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat
    14101 ggtaggttcc atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg
    14161 ccgttcttat aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc
    14221 tgaccgttat ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg
    14281 tcgcgtagct tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg
    14341 ctcctgtatg ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt
    14401 tgtacgtatc agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa
    14461 agagtccccg aactaccgtc tgggctcctg attctcttcg cccgacttca tgtcgggcga
    14521 tttatattat ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag
    14581 catcgcatcc tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg
    14641 cgtgagctgg gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt
    14701 gacttcaatc caagcggcat tattctttcc ggcggcccgg aaagtactac tgaagaaaac
    14761 agtccgcgtg cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat
    14821 ggcatgcaga ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa
    14881 tttggctacg cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat
    14941 gcgctgaccg cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt
    15001 accgctatcc cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt
    15061 atggctaacg aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc
    15121 cgccagggta tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg
    15181 tggacgccag cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac
    15241 gataaagtca tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg
    15301 caccgtgcta tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc
    15361 aacgaagcag agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac
    15421 gtaccggcag aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa
    15481 cgtaaaatca tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgca gaaactggaa
    15541 gacgtgaagt ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct
    15601 gcaaccggta aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag
    15661 atgaagatgg gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt
    15721 ggtctggagc tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc
    15781 cttggcgttc gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct
    15841 gacgccatct tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg
    15901 ttcaccgtct tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac
    15961 tgggttgtct ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat
    16021 ctgccgtacg atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt
    16081 tcccgcgtgg tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt
    16141 tgatccctgc actatgaatg aacaaaaccc tccgttacta cagagggttt tttatcttca
    16201 agaatcatag gcttgaagtt actaacatcg attaattaaa ccagctgtcc gatttgttct
    16261 cttctgcttt gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg
    16321 cgtcagcttc agattgcacc acatcggttt gcgcagcttt gtgcttcagt tcctgatcga
    16381 taaattcgtt ttcgcgctta acgcgggctt cttctttcgc cagcgccagt ttttgtttct
    16441 gaatctctaa gctgcgtagc tcatcttcat aactttgatc gcgttttttg tccgcagtgg
    16501 cttcggcgtc cagtttatcc tgacgagctt tcttatttgc cgctgccgtt gccgctcttt
    16561 tgttcgcggc ggcctgggca tttgcgcgac gttgcttctc ttgctggatt tccctgttgc
    16621 gctccgcgac ccattcgtca tgctgccttt gctcttcatt tttaccttgc tgttccgctt
    16681 ctg
//
LOCUS       DADLTF010000085        16384 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.085, whole genome shotgun sequence.
ACCESSION   DADLTF010000085 DADLTF010000000
VERSION     DADLTF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.085"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            7..375
                     /locus_tag="JN749_002590"
     CDS             7..375
                     /locus_tag="JN749_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005012176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368961.1"
                     /translation="MFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSER
                     PIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIGFF
                     GILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="JN749_002591"
     CDS             complement(411..1367)
                     /locus_tag="JN749_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HAY7368962.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JN749_002592"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="JN749_002592"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HAY7368963.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="JN749_002593"
     CDS             complement(2605..3366)
                     /locus_tag="JN749_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7368964.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="JN749_002594"
     CDS             3499..3909
                     /locus_tag="JN749_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368965.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="JN749_002595"
     CDS             complement(3871..4977)
                     /locus_tag="JN749_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7368966.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="JN749_002596"
     CDS             complement(4988..6121)
                     /locus_tag="JN749_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7368967.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="JN749_002597"
     CDS             complement(6114..7850)
                     /locus_tag="JN749_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7368968.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JN749_002598"
     CDS             complement(7843..8841)
                     /gene="hlyD"
                     /locus_tag="JN749_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HAY7368969.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JN749_002599"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="JN749_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HAY7368970.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10928
                     /gene="rhlE"
                     /locus_tag="JN749_002600"
                     /pseudo
     CDS             9741..10928
                     /gene="rhlE"
                     /locus_tag="JN749_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JN749_002601"
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="JN749_002601"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HAY7368971.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="JN749_002602"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="JN749_002602"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HAY7368972.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14877
                     /gene="ybiB"
                     /locus_tag="JN749_002603"
     CDS             13915..14877
                     /gene="ybiB"
                     /locus_tag="JN749_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HAY7368973.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15018..16103
                     /locus_tag="JN749_002604"
     CDS             15018..16103
                     /locus_tag="JN749_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HAY7368974.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHKETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
BASE COUNT         3825 a         4341 c         4441 g         3777 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggcgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggatct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gccctgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gacgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cttggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gcttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggatccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtggac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggccagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aagcagagat gctcggcttt
    14101 tacgaagcca tgcaaaatca caccatcaag ctgacgccgc ctgcaggcaa gccgatgccg
    14161 attgtcatcc ccagttacaa cggcgcgcgt aaacaggcta acctgacgcc attattggcg
    14221 attctgctgc ataaactcgg ttttcctgtg gtggttcacg gagttagcga agatccaact
    14281 cgcgtgctga cagaaaccat tttcgaactg atgggtatta caccaacgtt gcacggcggg
    14341 caggcgcagg cgaagctcga cgaacatcaa ccggtgttta tgcctgtcgg cgcgttttgc
    14401 ccgccgctgg aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat
    14461 accctggcga aactggcgac gccatttgcc gaaggtgagg cactgtgtct ttccagtgtt
    14521 tcgcatccgg aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggtgggcg
    14581 ttactaatgc atggtactga aggcgaagtg tatgctaatc cgcagcgctg cccgcagatc
    14641 aatctcattg accgtgaagg gatgcgggtg ctgtatgaaa aacaggaccc tgctggtagc
    14701 gagttactgc cacaagcaaa agatccggaa accacggcgc agtggactga gcgttgcctg
    14761 gctggcagcg aagcgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct
    14821 acgggtgaag cggcaactat cagcgatggc ctggcgcgcg ttaatcaggc attttaattt
    14881 ttatctcagc cggatgagcc acgtcatccg gcttttaccc caccgcaata tgaaattcct
    14941 gcatctttat tgaccttccc gcgcccggcg tgcagcataa aaaaacaaca aacacataac
    15001 ataaacagga gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt
    15061 ttattcaggc tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg
    15121 atcatttaat cgcggcaaac ctggcagggc atgattctca tggtattggc atgatcccaa
    15181 gctatgtgcg ttcctggagt caggggcatc tgcaaattaa ccatcatgcc aaaatcgtta
    15241 aagaggcggg ggcggcagtc acgctcgatg gcgatcgcgc attcggtcag gtcgcggcac
    15301 acgaagcgat ggcgctgggg attgagaaag cgcatcagca cggtattgct gccgtggcgc
    15361 tacataactc gcatcatatc ggccgtatcg gttactgggc ggagcagtgt tcagcggcgg
    15421 ggtttgtctc tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg
    15481 gtcgcgacag ccgctttggc accaatccct tctgtgtggt tttccctcgt aaagataatt
    15541 tcccgctgtt gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct
    15601 ggcataaagg cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga
    15661 ccaatccggc ggtaatgcag gagtcgccgt tggggtcgct gttgaccttt gccgaacata
    15721 aaggctacgc ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttct ggcggtaaaa
    15781 cgacgcataa ggaaacgttg caaaccagcc ccgatgccat tcttaactgc atgaccacta
    15841 tcatcatcaa cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg
    15901 ccgagtgggt gaaagcctcg ccgcatgatg acgataagcc gattttgcta ccgggcgagt
    15961 gggaagtgaa cacgcgtcgc gaacggcagg agcaggggat tccattggat gcgggaagct
    16021 ggcaggccat ttgtgatgcg gcgcggcaga ttggtatgtc ggaagagacg ttgcaggctt
    16081 tctgtcagca gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg
    16141 taacatagga tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata
    16201 cttgtgctgg acgtagcgta aacgcctgat ccgtccaacg gttcggattt tgtaggcctg
    16261 ataagacgcg gtagcgtcgc atcaggcatc tgttgccgga tgcggcataa acgccttatc
    16321 cgtcctacga attacttata aattaccgca gtaccgctta atttattgtt gttggtggcg
    16381 gaag
//
LOCUS       DADLTF010000086        16321 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.086, whole genome shotgun sequence.
ACCESSION   DADLTF010000086 DADLTF010000000
VERSION     DADLTF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.086"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(85..2061)
                     /gene="speA"
                     /locus_tag="JN749_002605"
     CDS             complement(85..2061)
                     /gene="speA"
                     /locus_tag="JN749_002605"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HAY7368975.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="JN749_002606"
     CDS             complement(2070..2201)
                     /gene="yqgB"
                     /locus_tag="JN749_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HAY7368976.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2337..2552
                     /locus_tag="JN749_002607"
     CDS             2337..2552
                     /locus_tag="JN749_002607"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7368977.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2856..4010
                     /gene="metK"
                     /locus_tag="JN749_002608"
     CDS             2856..4010
                     /gene="metK"
                     /locus_tag="JN749_002608"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAY7368978.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4447..5841
                     /locus_tag="JN749_002609"
     CDS             4447..5841
                     /locus_tag="JN749_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAY7368979.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWRTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5918..6415
                     /locus_tag="JN749_002610"
     CDS             5918..6415
                     /locus_tag="JN749_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HAY7368980.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6510..7217
                     /gene="endA"
                     /locus_tag="JN749_002611"
     CDS             6510..7217
                     /gene="endA"
                     /locus_tag="JN749_002611"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HAY7368981.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7297..8028
                     /gene="rsmE"
                     /locus_tag="JN749_002612"
     CDS             7297..8028
                     /gene="rsmE"
                     /locus_tag="JN749_002612"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAY7368982.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8041..8991
                     /gene="gshB"
                     /locus_tag="JN749_002613"
     CDS             8041..8991
                     /gene="gshB"
                     /locus_tag="JN749_002613"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HAY7368983.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9028..9663
                     /locus_tag="JN749_002614"
     CDS             9028..9663
                     /locus_tag="JN749_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HAY7368984.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9663..10079
                     /gene="ruvX"
                     /locus_tag="JN749_002615"
     CDS             9663..10079
                     /gene="ruvX"
                     /locus_tag="JN749_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAY7368985.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10255..11235)
                     /locus_tag="JN749_002616"
     CDS             complement(10255..11235)
                     /locus_tag="JN749_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HAY7368986.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11253..11957
                     /gene="yggS"
                     /locus_tag="JN749_002617"
     CDS             11253..11957
                     /gene="yggS"
                     /locus_tag="JN749_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HAY7368987.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11975..12541
                     /gene="yggT"
                     /locus_tag="JN749_002618"
     CDS             11975..12541
                     /gene="yggT"
                     /locus_tag="JN749_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HAY7368988.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12538..12828
                     /locus_tag="JN749_002619"
     CDS             12538..12828
                     /locus_tag="JN749_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HAY7368989.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANNHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12836..13429
                     /locus_tag="JN749_002620"
     CDS             12836..13429
                     /locus_tag="JN749_002620"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HAY7368990.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITHEPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13422..14558
                     /gene="hemW"
                     /locus_tag="JN749_002621"
     CDS             13422..14558
                     /gene="hemW"
                     /locus_tag="JN749_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HAY7368991.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14872..15835
                     /locus_tag="JN749_002622"
                     /pseudo
     CDS             14872..15835
                     /locus_tag="JN749_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15880..>16321
                     /locus_tag="JN749_002623"
     CDS             15880..>16321
                     /locus_tag="JN749_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAY7368992.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFY"
BASE COUNT         4039 a         4207 c         4107 g         3968 t
ORIGIN      
        1 cagctagatt atttttaaga atatataaaa agaaagcggg ccgatttacg taaataaatc
       61 gacccgccag cggaataatc ttaattattc atcttcaaga taagtataac cgtacaaacc
      121 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
      181 ttgatcgcgg aactgggtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      241 cgccacggta tcgccttcgt cagacagttc tacttctacg ctaccgtcag ggaacacgaa
      301 cacgtcaact gcttcggtat caccgaacag gttgtgcata ttgccaagga tctcctgata
      361 tgcaccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcattgg
      421 cattgtcgtg gcaataccat caccatcaat atagtggtcg atagcaccgt cagagtcaca
      481 ggtaatatcc agcagcacag cgcgacgttc cggcacttga tccagccctt ccagcggtag
      541 aaccgggaac aactggtcga tcccccatgc gtccggcatt gactggaaca gcgagaaatt
      601 gacgtacatt ttgtccgcca tacgttcctg cagttcgtcg attatcggac gatgcgcacg
      661 gttttgcgga tccagttgct tctgcacttc atggcacatg ctcagataga gttgttctgc
      721 ccatgcacgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      781 atccatctgg ctgtcgtgta accattcacg caaagaacgg cgagttcccg gttcgtgcat
      841 ttcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttctg cgggcgcggt
      901 cggcaccgta tattcgttac gttccacgcc gatgatattc gacaccagca cggtatggtg
      961 cgcagtcacc gcacggcccg attcggtgat taccgtcgga tgcggcagac cattttcttc
     1021 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
     1081 ggagcagtca gactgcgaac gggtgccttc ataatccacg cccagaccgc cgccgacgtc
     1141 gaagcactga atattgacgc ccagcttgtg cagttctaca tagaaacgcg cggattcacg
     1201 aacgcctgtc gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1261 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1321 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1381 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1441 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1501 ctcgccaatt aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1561 gctacgagtc atgccagcat gtgccagcac tgccatcaac tcggctttgg aaccggcttc
     1621 cagacccagc ggttcacccg aatgaatcag ggactcgatc acgcggcggt gctggttaac
     1681 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc tcgcacgttt
     1741 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1801 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1861 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1921 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1981 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     2041 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     2101 acgactgtcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2161 gtatgctgac gctccgactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2221 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2281 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2341 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca
     2401 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2461 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2521 cacgaaaaaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2581 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2641 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2701 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2761 tgtttttaac ctgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2821 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2881 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2941 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     3001 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     3061 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3121 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3181 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3241 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3301 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3361 tgactttcca gtatgacgac ggcaaaatcg ttggtattga tgctgtcgtg ctttccactc
     3421 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3481 agccaattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3541 gtcgtttcgt tatcggtggc ccaatgggtg actgcggtct gactggtcgt aaaattatcg
     3601 ttgataccta cggcggcatg gcgcgtcacg gtggcggtgc attctctggt aaagatccat
     3661 caaaagtgga ccgttccgca gcctacgcag cacgttatgt cgcgaaaaac atcgttgctg
     3721 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3781 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3841 tggtacgtga gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc
     3901 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3961 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg cctgaagtaa tctttcttca
     4021 cctgcgttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     4081 gcgttcacgc cgcatccggc aaaaaaactg cccgcacaat aacatcattc ttcctgatca
     4141 cgtttcacgg caaattacca tcacaattga aaccgattac accaaccaca ccagcaaaat
     4201 atttgtaata ttttcatatt attattcagc tttcacagtt gttacatttc ttttcagtaa
     4261 agtcttaatt gcagatgaca gcgtttaatc tatgatgata taactcaatt attttcatgc
     4321 acttaaatca taactaagat aaatgttagt gtaagcgatt acactgatgt gatttgcttc
     4381 acatcttttt acgtcgtact cacctatctt aattcacaat aaaaaataac catattggag
     4441 ggcatcatgc ctgacgctaa aaaacagggg cggtcaaaca aggcaatgac gtttttcgtc
     4501 tgcttccttg ccgctctggc gggattactc tttggcctgg atatcggtgt aattgctggc
     4561 gcactgccgt ttattgcaga tgaattccag ataacttcgc acacgcaaga atgggtcgta
     4621 agctccatga tgttcggtgc ggcagtcggt gcggtgggca tcggctggct ctcctttaaa
     4681 ctcgggcgca aaaagagcct gatgatcggc gcaatcctgt ttgttgccgg ttcactgttc
     4741 tctgcggctg cgccaaacgt tgaagtactg attctttcgc gcgttctgct ggggctggcg
     4801 gtgggtgtgg cctcttatac cgcaccactg tacctgtcag aaattgcgcc ggaaaaaatt
     4861 cgcggcagta tgatctcgat gtatcagttg atgatcacta tcgggatcct cggtgcttat
     4921 ctttctgata ccgccttcag ctacaccggt gcatggcgct ggatgctggg tgtgattatc
     4981 atcccggcaa ttttgctgct gattggtgtc ttcttcctgc cagacagccc acgttggttt
     5041 gccgccaaac gccgttttgt tgatgccgaa cgcgtgctgc tacgcctgcg tgacaccagc
     5101 gcggaagcga aacgcgaact ggatgaaatc cgtgaaagtt tgcaggttaa acagagtggc
     5161 tgggcgctgt ttaaagagaa tagcaacttc cgccgcgcgg tgttccttgg cgtactgtta
     5221 caggtaatgc agcaattcac cgggatgaac gtcatcatgt attacgcgcc gaaaatcttc
     5281 gaactggcgg gttataccaa caccaccgag caaatgtgga ggacagtgat tgtcggcctg
     5341 accaacgtac ttgccacctt tatcgcaatc ggccttgttg accgctgggg acgtaaacca
     5401 acgctaacgc tgggcttcct ggtgatggct gctggcatgg gcgtactcgg tacaatgatg
     5461 catatcggta tccactctcc gtcggcgcag tatttcgcca tcgccatgct gctgatgttt
     5521 attgtcggtt ttgccatgag tgccggtccg ctgatttggg tactgtgctc cgaaattcag
     5581 ccgctgaaag gccgcgattt tggcatcacc tgctccaccg ccaccaactg gattgccaac
     5641 atgatcgttg gcgcaacgtt cctgaccatg ctcaacacgc tgggtaacgc caacaccttc
     5701 tgggtgtatg cggctctgaa cgtactgttt atcctgctga cattgtggct ggtaccggaa
     5761 accaaacacg tttcgctgga acatattgaa cgtaatctga tgaaaggtcg taaactgcgc
     5821 gaaatcggcg ctcacgatta atctcctcag gcttcctccc gtctcggagg aagccgcctc
     5881 ttgcagtcat cttttcttcg ctctatcctc tgccgctatg aaaacatccc gtctccctat
     5941 agccatccaa caggccgtta tgcgccgcct gcgggaaaaa ctcgcccagg ccaacctgaa
     6001 gctagggcgt aactacccgg agccaaaact ctcttacacc cagcgcggaa cctccgccgg
     6061 aacagcctgg ctggaaagct atgaaatccg cctcaatccc gttttgctgt tggaaaacag
     6121 tgaagctttt attgaagaag tggtaccgca cgaactggca catttgctgg tatggaaaca
     6181 tttcggccgc gtagcgccac atggcaaaga gtggaagtgg atgatggaaa gcgtgctggg
     6241 cgttcccgcc cgtcgtacgc atcagttcga actgcaatcc gtgcgtcgca acaccttccc
     6301 ctaccgctgt aagtgccagg aacatcagct taccgtacgc cgccataatc gcgtagttcg
     6361 tggcgaggcc atctatcgct gtgttcactg cggtgaacag ctggttgcga aataaccacc
     6421 tgaactatca ggaactttcc tgatctggct gattgcatac caaaacaact ttcgctacgt
     6481 tgctggctcg ttttaacacg gagtaagtga tgtaccgtta tttgtctatt gctgcggtgg
     6541 tactgagcgc agcattttcc ggcccgacgt tggccgaagg tatcaatagt ttttctcagg
     6601 cgaaagccgc ggcggtaaaa gtccacgctg atgcgcccgg tacgttttat tgcggatgta
     6661 aaattaactg gcagggcaaa aaaggcgttg ttgatctgca atcgtgcggc tatcaggtgc
     6721 gcaaaaatga aaaccgcgcc agccgcgtag agtgggaaca cgtcgttccc gcctggcaat
     6781 tcggtcacca gcgccagtgc tggcaggacg gtggacgtaa aaactgcgct aaagatccgg
     6841 tctatcgcaa gatggaaagc gatatgcata acctgcagcc gtcagtcggc gaggtgaatg
     6901 gcgatcgcgg caattttatg tacagccagt ggaatggcgg cgaaggccag tacggtcaat
     6961 gcgccatgaa ggtcgatttc aaagaaaaag ctgccgaacc accagcgcgt gcacgcggtg
     7021 ccattgcgcg cacctacttc tatatgcgcg accaatacaa cctgacactc tctcgccagc
     7081 aaacgcagct gttcaacgca tggaacaaga tgtatccggt taccgactgg gagtgcgagc
     7141 gcgatgaacg catcgcgaag gtgcagggca atcataaccc gtatgtgcaa cgcgcttgcc
     7201 aggcgcgaaa gagctaacct acactagcgg gattcttttt gttaacccct accccacgcg
     7261 tacaaccgcg tggggagacg acgcggattt ttaactatgc gtatcccccg catttatcat
     7321 cctgaaccac tgaccagcca ttctcacatc gcgctttgcg aagatgccgc caaccatatc
     7381 gggcgcgtac tgcgcatggg gccggggcag gcgttgcaat tgtttgacgg tagcaaccag
     7441 gtctttgacg ccgaaattac cagcgccagc aaaaaaagcg tggaagtgaa ggtgctggaa
     7501 ggccagatcg acgatcgcga atctccgctg catattcacc tcggtcaggt gatgtcgcgt
     7561 ggtgaaaaaa tggaatttac tatccagaaa tcgatcgaac tcggtgtaag cctcattacg
     7621 ccactttttt ctgagcgctg cggcgttaaa ctggatagtg aacgtctgaa caagaagctt
     7681 cagcagtggc agaagattgc aattgctgcc tgtgagcagt gtggtcgtaa ccgggtgccg
     7741 gaaatccgtc cagcgatgga tctggaagcc tggtgtgcag agcaggatga aggactgaaa
     7801 ctgaatcttc acccgcgcgc cagtaacagc atcaatacgt tgcctttacc ggttgaacgc
     7861 gtccgcctgc tgattggccc ggaaggcggt ttatcggcag atgaaattgc catgactgcc
     7921 cgctatcaat ttactgatat cctgttggga cctcgcgttt tgcgtacaga gacaactgcg
     7981 ctcaccgcca ttaccgcgct acaagtacga tttggcgatt tgggctaacg gagaagaata
     8041 atgatcaagc tcggcatcgt gatggacccc atcgcaaaca tcaacatcaa gaaagattcc
     8101 agtttcgcta tgttgctgga agcacagcgt cgtggttacg aacttcacta tatggagatg
     8161 ggcgatctgt atctgatcaa tggcgaagcc cgcgcccata cccgcacgct gaacgtgaag
     8221 cagaactacg aagagtggtt ttcgttcgtc ggtgaacagg atctgccgct ggccgatctc
     8281 gatgtgatcc tgatgcgtaa agacccgccg tttgataccg agtttatcta cgcgacctat
     8341 attctggaac gtgccgaaga gaaagggacg ctgatcgtta acaagccgca gagcctgcgc
     8401 gactgtaacg agaaactgtt taccgcctgg ttctctgact taacgccaga aacgctggtt
     8461 acgcgcaata aagcgcagct aaaagcgttc tgggagaaac acagcgacat cattcttaag
     8521 ccgctggacg gtatgggcgg cgcgtcgatt ttccgcgtga aagaaggcga tccaaacctc
     8581 ggcgtgattg ccgaaaccct gactgagcat ggcactcgct actgcatggc gcaaaattac
     8641 ctgccagcca ttaaagatgg cgacaaacgc gtgctggtgg tggatggcga gccggtaccg
     8701 tactgcctgg cgcgtattcc gcaggggggc gaaacccgtg gcaatctggc tgccggtggt
     8761 cgcggtgaac ctcgtccgct gacggaaagt gactggaaaa tcgcccgtca gatcgggccg
     8821 acgctgaaag aaaaagggct gatttttgtt ggtctggata tcatcggcga ccgtctgact
     8881 gaaattaacg tcaccagccc aacctgtatt cgtgagattg aagcagagtt tccggtgtcg
     8941 atcaccggaa tgttaatgga tgccatcgaa gcacgtttac agcagcagta acccacctta
     9001 gcgagaagga tctcgttgag actctgagtg acagcgccct tctttccacg catactgggc
     9061 gctgttgctt ttttgaacca ggaaacagaa cctctgacaa tgaatttaca gcatcacttt
     9121 cttattgcca tgcctgctct ccaggatccg attttccgtc gttccgtggt ctacatttgc
     9181 gaacataata ccaatggtgc aatggggatc atcgtcaaca agccgctgga aaatctcaaa
     9241 attgaaggga ttctggaaaa gctgaagatc acgccggagc cgcgtgatga atcaatccgt
     9301 ctggataaac cggttatgct cggcggccca ctggctgaag atcgcgggtt tattttgcat
     9361 actccgccct ccaattttgc ttccagcatt cgcatttcag acaacacggt gatgaccact
     9421 tcccgcgatg tgctggaaac gctcggcacc gataaacaac cgtctgacgt attggtggct
     9481 ctgggctatg cctcctggga aaaaggtcaa ctggaacaag aaattctcga taacgcgtgg
     9541 ctaacggccc cggcggatct gaatattctg ttcaaaacgc cgattgccga ccgctggcgc
     9601 gaggcggcaa aactgattgg tgtggatatt ctcaccatgc ctggtgtggc aggacacgcc
     9661 tgatgagtgg aaccttactc gccttcgact tcggcaccaa aagcattggc gtagcggtcg
     9721 gccaacgcat taccggcacc gctcgccctt tgcctgcaat taaagcgcag gacggtacgc
     9781 cggactggaa cctcatcgaa cgtttactga aagagtggca gcctgacgaa atcatcgtcg
     9841 gtttgccgct gaatatggac ggcaccgaac agccgttaac cgccagagcg cgcaaatttg
     9901 ccaaccgtat tcatggtcgt ttcggtgttg aagtaaagct ccatgacgag cgtcttagca
     9961 ctgtggaagc ccgttccggt ctttttgaac agggcggcta tcgggcgctc aacaaaggca
    10021 aagttgactc tgcctctgcg gttattattc tcgaaagcta tttcgagcag ggatattaag
    10081 gcgatctaaa agcctggcgg atagtcgtta ccgccatctt gccggatgcg gcgtgaacgc
    10141 cttatccagc acacatctgg cagcggctgt aggtctgata agacgcgtta gcgtcgcatc
    10201 agacatttat tgcctttata ggccctataa acttgcgcat cgggcatggc aacgtcacaa
    10261 acgcccttcc cccacccgct gctgataact ctgctgaaac gttaacatcc ccacctgctg
    10321 cccggtttga ataacatgcg gtaactggtg ggttttccct tcgcgaatca aattccccac
    10381 cgcgggagtg ttaatcagta attcaaacag cgccacgcgt ccttcctgtt tatccacttc
    10441 cagcttttgt gacagcactg cccgtaaact acccgcaagt tgattacgta cgggatcttt
    10501 ttcctgcgcc ggaaatgaat ccaccagtcg ctcaactgcc tgcgcggcac cacgtgtatg
    10561 taaggttgcc agcaccagat gcccggtttc tgccgccgtc agcgccagac ggattgtctc
    10621 actgtcacgt agctctccga gcaaaatcac atcaggatct tcccgcaatg cagcccgcaa
    10681 ccccgatgcg aacgtcatac agtgcaaacc aatttcccgc tgctggatca aacatcgctg
    10741 gctggcatag agatattcca caggatcttc cagcgtcaga atatgcgcat cggcatgttg
    10801 attaagatag ccaaccatcg ccgccagcgt ggtagatttg ccactccccg tagcccccgt
    10861 caccagaatc aggccattct cgctcttgag taattccggc aataccggtg gcgcaccaag
    10921 ctgttcgagc tgtggacagt gcgaaggtaa caaccgtaat gccagagaaa taccttgccg
    10981 ttgcgcgaac gcactgccac gcaaccgctg gttttccgcc agcgacacgg caaaatccag
    11041 ctggccattt tccagcaata ttgcccgctg atcgtcatcc agccactccc gcagtagctc
    11101 ttcgacgtcc ggcgcatcaa acggcgcagc ttccattctg ccacgaatgc gccatcgtgc
    11161 gggccaggcg ctgcacaggt gtagatccga gacgttatgc tttacactaa gggccacaat
    11221 ttcttccata ttcatactaa gatcctcgga aaatgaacga tattgcgcat aacctggcac
    11281 aggtccggga caaaatctca gcggctgcaa cgcgttgcgg ccgttctcca gaagaaatta
    11341 cgctgcttgc agtcagtaaa acaaaacctg cgagcgccat cgcagaagcc attgatgccg
    11401 ggcagcgtca atttggtgaa aactacgttc aggaaggggt agataaaatt cgccactttc
    11461 aggaactggg cgtaacagga ttagaatggc attttattgg cccgttgcag tctaataaaa
    11521 gccgcctagt ggcagagcat ttcgactggt gtcataccat cgaccgtttg cgcattgcta
    11581 cccgcctcaa cgatcagcgc ccggcagaac ttccccctct taacgtactg attcaaatta
    11641 atattagtga tgaaaacagt aagtccggga ttcaactggc agaactggac gagctggcag
    11701 cggcggtcgc tgaactaccg cgtttacgtc tgcgcgggct gatggcaatc cctgcgcctg
    11761 agtcagaata tgtaaggcag tttgaagttg cacgccaaat ggctgtagca tttgccggac
    11821 tgaaaacgcg ctacccgcat atcgacacgc tctctctggg aatgtcggac gatatggaag
    11881 ccgccattgc ggcaggtagc acgatggttc gtatcggcac tgcaattttt ggtgcgcgtg
    11941 attactctaa aaaataagga attaaaggaa cgccatgaat acgttgactt tcctgctttc
    12001 aacggtcatt gagctgtata ccatggtgct gttattacgc atctggatgc agtgggctca
    12061 ttgtgatttt tacaacccct tctcacagtt tgtagtgaag gtagcgcagc caattatcgg
    12121 gccactgcgc cgcgttattc cggcaatggg tcccattgac agcgcctcgc tgctggttgc
    12181 ctatattctc agttttatca aagccattgt gctgtttgaa gtggtgacct tcctgccaat
    12241 catctggatt gccggtttac tgattctgct gaaaaccatc ggcttgctga ttttctgggt
    12301 cctgctggtg atggcgatta tgagctgggt aagccagggt cgtagcccga ttgaatacgt
    12361 gctgattcag ctggccgatc cgctgctgcg cccgattcgc cgcttgctac cggcaatggg
    12421 tgggattgat ttctcgccga tgatcctcgt tctgctgctg tatgtcatca atatgggtgt
    12481 cgcagaagta ttacaggcaa ccggaaacat gctgctgccg gggctgtgga tggcgttatg
    12541 agtgccgtaa cagttaatga tgacggtctg gttttacggc tctatattca gccgaaagcc
    12601 agccgtgatt ctattgtcgg tttacatggc gacgaagtta aagtcgccat taccgcgccg
    12661 ccggttgacg gccaggccaa caatcatctg gtgaagtttc tcggtaagca attccgggtt
    12721 gccaaaagcc aggtggtgat tgaaaaaggc gaacttggcc gccacaaaca aattaaaatc
    12781 attaatccgc aacaaatccc gccagaaatc gcggcgttaa ttaattaggt atcctatgca
    12841 aaaagttgtc ctcgcaaccg gcaatgccgg taaagtgcgt gagctggcgt cgctgcttag
    12901 cgactttggt cttgatatcg tggcccaaac agacctcggc gttgattccg ctgaagaaac
    12961 cggcctaacc tttatcgaaa acgcgattct gaaagcgcgc catgcggcaa aagtgaccgg
    13021 tttaccggca attgccgacg actctggtct ggcggtagat gcgctgggtg gcgcgccggg
    13081 tatttactcc gcgcgttatt ccggtgaaga cgcgaccgat caaaagaatc tgcaaaaact
    13141 gttggaaaca ctgaaagacg taccggacga ccaacgtcag gcgcgtttcc actgcgtgct
    13201 ggtatatctg cgtcacgcgg aagatccgac tccgctggta tgccacggca gctggccggg
    13261 cgtgattact cacgaaccag cgggcactgg tggctttggt tatgatccaa tcttcttcgt
    13321 accttccgaa gggaaaaccg ccgccgaact gacccgcgaa gaaaagagcg ccatttccca
    13381 ccgtggtcag gcgttgaaac tgctgctgga cgctttacgt aatggttaaa ttaccgccgc
    13441 tgagtctcta cattcacatc ccgtggtgcg tgcagaaatg cccgtactgc gatttcaact
    13501 ctcacgcgtt gaaaggagaa gtgccgcacg acgattatgt tcagcatctg ctttgcgatc
    13561 tggacaatga tgtggcttac gctcagggcc gtgaagtaaa gacaatcttt attggcggtg
    13621 gtacgccgag cctgctttcc ggcccggcga tgcaaacgct gctagacggc gtgcgttcgc
    13681 gtttgccgct ggcagcggat gcagaaatta ctatggaagc gaacccaggc acggtagaag
    13741 cggatcgctt tgtcgattat cagcgtgctg gtgtaaaccg catctctatt ggtgtgcaga
    13801 gttttagcga agaaaagctg aaacgacttg ggcgtattca tggcccgcaa gaagcgaaac
    13861 gagcggcaaa gctggcgagc ggtttagggt tacgtagctt taaccttgat ttgatgcatg
    13921 gactaccgga tcaatcactg gaagaggcgc ttggcgatct acgccaggcc attgaactga
    13981 atccgccgca tctttcctgg tatcaactga ccatcgaacc taatacgctg tttggctcgc
    14041 gaccgccggt gctgccggac gatgacgcgc tgtgggatat tttcgaacag gggcatcagt
    14101 tattaaccgc agcgggttat cagcaatatg aaacttccgc ttacgccaaa cccggttatc
    14161 agtgccagca caatctcaac tactggcgct ttggtgacta catcggtatt ggctgcggcg
    14221 cgcacggcaa agtgaccttc ccggatgggc gcattctgcg taccactaaa acgcgtcatc
    14281 ctcgtggttt tatgcaagga aggtatctgg aaagccagcg tgatgtcgaa gccgcagata
    14341 agccgtttga gttctttatg aatcgcttcc gtctgctgga ggccgcgccg cgcgtggagt
    14401 ttattgcgta taccgggctt tgcgaagatg tgattcgccc acagttagac gaggcgattg
    14461 cccagggtta tctcaccgaa tgtgcggatt actggcagat aacggaacat ggtaaattat
    14521 tcttaaactc acttcttgaa ttattcctcg ctgagtaatc attaatgcgc ctgcgggcgc
    14581 attaatcact tcttttatcc ccccgttaat taacctcggc agaaatatat ttcttactga
    14641 ataaatatcg acgacattta ttatatatat ttagtgaata tttactaatc attcattgat
    14701 tataaatatg tacaacctgg cgcacaaatt taatatttaa tttctgactt tatttcaagt
    14761 tgtgataaac atagacaagt tgtcagacag gttaaccaat ttgtcgcaca aagcactgat
    14821 aagcacaatt agatatcaac caatttccat ccttcaacag ggagtttttt tatgaaaatg
    14881 aggtcattta cccgctcatt agtctgcgca tccttgctgg ctctggttag cactggcgta
    14941 aacgccgcag aaaaagtgac gttaaagtta gcacacaacc tggaaagaag tcatgttgta
    15001 catcagagtt ttgaagagct ggcgaaagag gtaaaacagc tgtcaaaagg taatatggtg
    15061 atccgtattt atcccagcag ccagatgggt aatgcgcgag aaactatgga attgttgcaa
    15121 aatggcgcac tggatatgac caaaggttcc gccagtgatc tggagtcttt tgataatatt
    15181 tatgctatct ataatttgcc gttcttatta agttgttttt ggtgaagttg gtaaagaaat
    15241 tatggactca acaaaagaca aaggcttttt tgctttatca gcttatgtag cgggtactcg
    15301 cagtttctat gcgaaaaaac cgattacaaa gccagaagat cttaaaggac tgaaaattcg
    15361 tgttcagcca agtccgacca ccatcaaaat gattgaactg atgggtggtt cacccactcc
    15421 aatttcattt ggcgaagttt atactgcgat gcagcaaggt gtagtagatg gcgcagaaaa
    15481 taacgtgcct tcctggatgc agactcgaca tattgaaatt gccaaagttt tttctgaaga
    15541 tgaacacgcc tcaattcctg actttttggt tatctccacc aaaacatgga ataaattacc
    15601 cccagaacag caacaaatcc tcgaaacagc ggcgaagaaa tccgaagctt atcaacaaaa
    15661 attgtgggaa aaaattgacg ctgacacacg tgctcaggcg aaagctatgg ggggtgaaat
    15721 tgtaaaagtg gataaagcgc cattccgtaa agccgtgcaa cctctgtttg acgacttcaa
    15781 aaaagatcca aaacaagctg ctttgttaga gaaattcgac aacgctgctc aataatattc
    15841 cgggccagcc tctggccctg attttccagg atgcttacaa tgatatttaa ccgcctgaaa
    15901 cttgcggtag accgcgtcat tgccgcgttt tctgtcgccg taatgcttgc tctggttgta
    15961 tgcgtagtct ggcaagtatt tagccgctac gtacttaacc agccaagtac attaaccgat
    16021 gagctggcac gctttttgat gatctgggta ggattattag gtgctgcata taccgtcggc
    16081 gcgcagcgac atctctctat tgatttattt gctctcgccc tgaataagcg caaacaatta
    16141 cttcttagta tagtcattaa cgttctcatt ttaggttttg ccggttcggt cattgtgact
    16201 ggtggcctga aattaattga taaaacactg gcaacatcac aggtatctgc cgctatgcaa
    16261 ataccgatgg gatacgtata tatcattctg ccattaagtg gagtggtaat gatgttttat
    16321 g
//
LOCUS       DADLTF010000087        16148 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.087, whole genome shotgun sequence.
ACCESSION   DADLTF010000087 DADLTF010000000
VERSION     DADLTF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16148
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.087"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(188..520)
                     /gene="emrE"
                     /locus_tag="JN749_002624"
     CDS             complement(188..520)
                     /gene="emrE"
                     /locus_tag="JN749_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HAY7368993.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(865..1179)
                     /gene="fliE"
                     /locus_tag="JN749_002625"
     CDS             complement(865..1179)
                     /gene="fliE"
                     /locus_tag="JN749_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HAY7368994.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1393..3051
                     /gene="fliF"
                     /locus_tag="JN749_002626"
                     /pseudo
     CDS             1393..3051
                     /gene="fliF"
                     /locus_tag="JN749_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
     gene            3044..4039
                     /gene="fliG"
                     /locus_tag="JN749_002627"
     CDS             3044..4039
                     /gene="fliG"
                     /locus_tag="JN749_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HAY7368995.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4032..4718
                     /gene="fliH"
                     /locus_tag="JN749_002628"
     CDS             4032..4718
                     /gene="fliH"
                     /locus_tag="JN749_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HAY7368996.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            4718..6091
                     /gene="fliI"
                     /locus_tag="JN749_002629"
     CDS             4718..6091
                     /gene="fliI"
                     /locus_tag="JN749_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HAY7368997.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRSQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6110..6553
                     /gene="fliJ"
                     /locus_tag="JN749_002630"
     CDS             6110..6553
                     /gene="fliJ"
                     /locus_tag="JN749_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HAY7368998.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6550..7677
                     /gene="fliK"
                     /locus_tag="JN749_002631"
     CDS             6550..7677
                     /gene="fliK"
                     /locus_tag="JN749_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HAY7368999.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSASLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGDDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7782..8246
                     /gene="fliL"
                     /locus_tag="JN749_002632"
     CDS             7782..8246
                     /gene="fliL"
                     /locus_tag="JN749_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HAY7369000.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8251..9255
                     /gene="fliM"
                     /locus_tag="JN749_002633"
     CDS             8251..9255
                     /gene="fliM"
                     /locus_tag="JN749_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HAY7369001.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDILPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9252..9665
                     /gene="fliN"
                     /locus_tag="JN749_002634"
     CDS             9252..9665
                     /gene="fliN"
                     /locus_tag="JN749_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HAY7369002.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9668..10033
                     /gene="fliO"
                     /locus_tag="JN749_002635"
     CDS             9668..10033
                     /gene="fliO"
                     /locus_tag="JN749_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HAY7369003.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            10033..10770
                     /gene="fliP"
                     /locus_tag="JN749_002636"
     CDS             10033..10770
                     /gene="fliP"
                     /locus_tag="JN749_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HAY7369004.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10780..11049
                     /gene="fliQ"
                     /locus_tag="JN749_002637"
     CDS             10780..11049
                     /gene="fliQ"
                     /locus_tag="JN749_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HAY7369005.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11058..11762
                     /locus_tag="JN749_002638"
     CDS             11058..11762
                     /locus_tag="JN749_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HAY7369006.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFNGHLWLISLLVDTFHTLPIGSEPLNSNAFLAPTKAGSLIFLNGLMLA
                     LPLITLLLTLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFS
                     EIFNLLADIISELPLI"
     gene            12047..12670
                     /gene="rcsA"
                     /locus_tag="JN749_002639"
     CDS             12047..12670
                     /gene="rcsA"
                     /locus_tag="JN749_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HAY7369007.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12714..12956)
                     /gene="dsrB"
                     /locus_tag="JN749_002640"
     CDS             complement(12714..12956)
                     /gene="dsrB"
                     /locus_tag="JN749_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HAY7369008.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDSGPRRPGVVLAVEE
                     FSEGTMYLVALEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            13065..13292
                     /gene="yodD"
                     /locus_tag="JN749_002641"
     CDS             13065..13292
                     /gene="yodD"
                     /locus_tag="JN749_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HAY7369009.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13590..14405
                     /locus_tag="JN749_002642"
                     /pseudo
     CDS             13590..14405
                     /locus_tag="JN749_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="JN749_002643"
     CDS             complement(14402..16096)
                     /gene="yedQ"
                     /locus_tag="JN749_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HAY7369010.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDHA"
BASE COUNT         4022 a         4155 c         4236 g         3735 t
ORIGIN      
        1 aaacgataag ttttttcata aaaacctcat tgttgtgacc tatcgttatt ttatttgatg
       61 tgccttgatc tcattatgca taaaggcaca atgatcgcgc aaatgcaatc ataattataa
      121 aggtgccccg aacattagcc ataatatgca caaatcagat tcctgttatc tcaaggagaa
      181 ttatttttta atgcggtgca cttcgtgaca acaagttaat gactaacaca ccggcacaaa
      241 tcaacatcat gcctatgata gctggcaggt ccagtcgttg accaaaaaat ccccaggaaa
      301 gcaaactaat cagaacgata ccgactcctg accagatagc ataagcaatc cctgtaggta
      361 tataagccag cgtctgagct aataaccaga atgatgcaca atagcaaata attgtgccaa
      421 cagagggcca taaccgtgta aacccctctg aaaacttcat taaggttgta ccaatgacct
      481 ctgcaagtat tgcgccacca agataaatat aagggttcat agcatattct ttcctgttca
      541 tactggagag aattgtacta cagtttgaac acaactcacc gttttcatcc tagtgtatgt
      601 gtatccaagg ggctctggcg tggcaagaga taaaggcagg aacaataatg atcagaagga
      661 accccaggct aacgaatgag ttcactcttc ctgggagcaa agcgaagaca aaacatgtta
      721 tcgcgtttac ggatcgtgga tggcagaaaa caaccaggat caggtatcca ttttaaacca
      781 gaaattgagt gagtttgccc catccatgcc ccacgctgtg ggatcggata taattaaaca
      841 agcatagtca acaggttaca caacctacac ctgcatagac atcacttcct gatacgccgc
      901 caccagctta ttacgcacct gaatccccat ttgcatagaa actgaggctt tttgcatatc
      961 ggtcatcaca tcgtttagcg ccacgccggg ttcaccgagg gtgaattttt ctgcctgcgt
     1021 gcgggcgact gtttgtgtat cgcttatgcg atcgatcgcg gcgtgcagct gcccggcaaa
     1081 actaatggtc ggttgcggca gtgattcctg cgcacgcgca ctcatcgccg tagcctgtaa
     1141 ctggctgata accccttcaa tcccctgtat cgctgacatt ttcgtctccc ggataatttc
     1201 tggtaacaaa gcctaccagt aagtcaataa gacaaaggcg ctaaatagca acaaaaaaac
     1261 gggtttattg gcggatagaa aaaacgaaag cacaaataat gggagcgtca atttttcgag
     1321 tttgctgacc cgagagtgag tcttgttcca ctttgccaat aacgccgtcc ataatcagcc
     1381 acgaggtgcg cgatgaatgc gactgcagcc cagacaaaat ctcttgagtg gcttaatcgc
     1441 ctgcgtgcga atccgaaaat tccattgatt gttaccggtt ccgcggcagt ggcggtcatg
     1501 gtcgcactga tcctgtgggc gaaagccccc gactaccgca cattattcag caatctttcc
     1561 gatcaggatg gtggcgcaat tgtcagccaa ctgacgcaaa tgaatattcc ttaccgcttc
     1621 agcgaagcca gcggcgctat tgaggttccg gcagataaag ttcacgaact gcgcctgcgc
     1681 ctggcgcaac aaggtttgcc caaaggcggc gcggtcggtt tcgaactgct cgatcaggaa
     1741 aagtttggta tcagccagtt cagcgaacag gtgaattatc agcgggcgct ggaaggcgag
     1801 ctttctcgta ccatcgaaac tatcggcccg gtaaaagggg cgcgcgtaca tctggcaatg
     1861 ccgaaaccgt ctttattcgt ccgtgaacaa aaatcccctt ctgcatcggt gacggtaaat
     1921 ctgttaccca gccgcgcact cgatgaaggg caaattagcg ctattgtaca tctggtttcc
     1981 agcgcggttg ctggtctgcc gcctggaaac gtcacgctgg tggatcaggg cgggcatctg
     2041 ttaacccagt ccaataccag cgggcgcgat cttaatgacg ctcagttgaa atatgccagc
     2101 gatgtcgaag gccgtattca gcggcgtatt gaagcgatcc tgtcgcctat tgttggtaac
     2161 ggtaatattc acgcctaggt cacggcgcaa ctggacttcg ccagtaaaga acaaacggaa
     2221 gaacagtatc gccctaacgg tgatgaatct catgcggcgc ttcgttcacg ccagcttaat
     2281 gagagcgagc aaagcggttc cggttatccg ggcggcgtac cgggggcgtt gtcgaatcaa
     2341 ccggcacctg cgaataacgc gccaatcagc acgcctccga caaatcaaaa taaccgccag
     2401 cagcaggcga gcaccaccag caatagtggg ccgcgtagca cacagcggaa tgaaaccagt
     2461 aactacgaag tcgatcgcac cattcgtcat accaaaatga acgtgggcga tgtgcaacgt
     2521 ctgtcagtcg cggtagtggt gaattacaaa accttgccag acggtaaacc attgcctctc
     2581 agcaacgaac agatgaagca aattgaagct ctgacccgcg aggcgatggg cttttctgaa
     2641 aaacgcggtg attcgctcaa tgttgttaac tcgccgttca atagcagtga cgaaagcggc
     2701 ggagcactgc cgttttggca acagcaagtg tttatcgatc agttgctggc tgccggtcgc
     2761 tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta
     2821 acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg
     2881 gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     2941 caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     3001 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     3061 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     3121 caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca
     3181 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
     3241 tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
     3301 tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg
     3361 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
     3421 tccgcaaatt atcgccacca ttctggtgca tctgaagcgc gcccaggccg ccgatattct
     3481 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
     3541 cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca
     3601 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
     3661 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
     3721 gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
     3781 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
     3841 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
     3901 gcgcgacgat ctcgccaacc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa
     3961 agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg
     4021 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
     4081 caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
     4141 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc
     4201 aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg
     4261 cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc
     4321 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag
     4381 cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa
     4441 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
     4501 tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc
     4561 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg
     4621 gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
     4681 aagaactctg ccgtctggca gcactaggag tggtgtaatg accacgcgcc tgactcgatg
     4741 gctaaccacg ctggataact ttgaagcaaa aatggcgcag ttgcctgcgg tacgtcgcta
     4801 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
     4861 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
     4921 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt
     4981 cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa
     5041 gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct
     5101 cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
     5161 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
     5221 cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa
     5281 aagtgtgctg ctggggatga tggcacgtta cacccgcgcc gatgtcattg tcgtgggttt
     5341 gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
     5401 acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
     5461 aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtagtc agcatgtgtt
     5521 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
     5581 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
     5641 ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
     5701 gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga
     5761 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
     5821 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
     5881 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
     5941 cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct
     6001 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
     6061 gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaca
     6121 tggtgcgctg gcgaccctga aagatctggc agaaaaagag gtagaggatg ccgctcgcct
     6181 gctgggtgaa atgcgtcgcg gatgtcagca ggcggaagaa cagctcaaaa tgctgatcga
     6241 ttatcagaat gaatatcgca ataacctcaa tagcgatatg agtgccggga tgaccagcaa
     6301 ccgctggatc aactatcagc agtttatcca gacgctggaa aaagccatta ctcagcatcg
     6361 ccagcaactt aatcagtgga cgcagaaagt tgacattgcc ctgaacagct ggcgagaaaa
     6421 gaaacaacgt ttgcaggcct ggcagacact gcaggaacgg caatccacgg cggcactgct
     6481 tgcagaaaac cgcctcgatc agaaaaagat ggatgagttc gcccagcgcg ccgccatgag
     6541 gaaacctgaa tgattcgctt agcgcccttg attaccgccg acgttgacac caccacattg
     6601 cctggcggca aagccagcga tgctgcacaa gattttctaa cgttgttgag cgaagcatta
     6661 gcaggcgaga caacaactga caaagcggcc ccccagttgc tggtggcaac agataagccc
     6721 acgacaaaag gcgagccgct ggtcagcgat attcttgccg atgcgcaaca agcggattta
     6781 ctgatccctg tggatgaaac acttcctgtc atcaacgacg aacaatccac atcaacaccg
     6841 ttaaccaccg ctcagacaat gacgttggct gcggtggctg acaaaaatac gacaaaagac
     6901 gaaaaagcgg atgatcttaa tgaagacgtc accgcaagtc tgagcgccct ttttgcgatg
     6961 ttgccgggtt ttgacaatac gcccaaagtg actgatgcac cgtcaaccgt gttaccggca
     7021 gagaaaccaa cgctcttcac aaaactgact tctgcgcaac tcactacagc acagcctgat
     7081 gacgcccccg gcacaccagc tcagccatta acaccgctgg tagcagaagc ccagagtaaa
     7141 gcggaagtca tcagcacgcc ttcgccggtg accgctgacg ccagcccgct aatcactcca
     7201 caccagacac agccactgcc caccgtcgcc gcgcctgttt tgagtgcatc gctgggttct
     7261 cacgaatggc aacaatcatt gagccagcat atttcgctgt tcacccgcca ggggcaacaa
     7321 agtgcagagt tgcgtctgca cccacaggat ttaggtgaag tgcaaatttc cctcaaagtg
     7381 gatgataacc aggcgcagat ccagatgatt tcaccgcatc agcacgtacg cgccgccctg
     7441 gaagcagcgc tgccggtact gcgtacgcag ctggccgaaa gtggcattca gttagggcaa
     7501 agcaacatta gtggcgaaag ctttagtggt cagcagcagg ccgcttccca gcaacagcaa
     7561 agccaacgca cagcaaacca tgaacctctg gcgggggatg acgacgatac gcttccggtt
     7621 cccgtctctt tacaagggcg tgtaacaggt aacagcggcg ttgatatttt cgcctaacgt
     7681 cagaggtagc accgtaatcc gcgtcttttc cccgctttgt tgcgctcaag acgcaggata
     7741 attagccgat aagcagtagc gacacaggaa gaccgcaaca catgactgat tacgcgataa
     7801 gcaagaaaag caagcgatcg ctttggatcc cgattctggt attcattacc ctcgcggcct
     7861 gtgccagcgc aggttacagc tactggcatt cgcatcaggt tgccgctgac gacaaagcgc
     7921 agcaacgcgt cgtgccctcc ccggtcttct atgcgctgga tacctttacg gtcaatttgg
     7981 gcgatgcgga tcgcgtgctt tatatcggca taaccctgcg actgaaagat gaagcaaccc
     8041 gctcgcggct gagtgagtat ttgccggaag tccgtagtcg cttgctgtta ctgttttcac
     8101 gtcaggatgc tgccgtactg gcgacagaag aaggcaagaa aaacctgatt gccgagatta
     8161 aaaccacact ttccaccccg cttgttgccg ggcaaccgaa acaggatgtc accgacgtgc
     8221 tgtataccgc ttttattctg cgataacgac atgggcgata gtattctttc tcaagctgaa
     8281 attgatgcgc tgttgaatgg tgacagcgaa gtcaaagacg aaccgacagc cagtgttagc
     8341 ggcgaaagtg acattcgtcc gtacgatccg aatacccaac gacgggttgt gcgcgaacgt
     8401 ttgcaggcgc tggaaatcat taatgagcgc tttgcccgcc attttcgtat ggggctgttc
     8461 aacctgctgc gccgtagccc ggacattacc gtcggggcca tccgcattca gccataccat
     8521 gaatttgccc gcaacctgcc ggtgccgacc aacctgaacc taatccacct gaaaccgcta
     8581 cgcggcacag ggctggtagt gttctcgccg agtctggtgt ttatcgccgt ggataacctg
     8641 tttggcggcg atggacgctt cccgaccaaa gtggaaggtc gcgagtttac ccatactgaa
     8701 cagcgcgtca tcaaccgtat gttgaagctg gcgctggaag gctatagcga cgcctggaaa
     8761 gcgattaatc cgctggaagt tgagtacgtg cgttcggaaa tgcaggtgga atttaccaat
     8821 atcaccacct cgccgaacga cattgtggtt aacacgccgt tccatgtgga gattggcaac
     8881 ctgaccggcg aatttaatat ctgcctgcca ttcagcatga tcgagccgct acgggaattg
     8941 ttggttaacc cgccgctgga aaactcgcgt aatgaagatc agaactggcg cgataacctg
     9001 gtgcgccagg tgcagcattc acagctggaa ctggtggcca actttgccga tatctcgcta
     9061 cgcctgtcgc agattttaaa actgaaaccc ggcgacatcc tgccgataga aaaacccgat
     9121 cgcatcatcg cccatgttga cggcgtcccg gttctgacca gtcagtacgg cactctcaac
     9181 ggtcagtatg cgttacggat agaacatttg attaacccga ttttaaattc tctgaacgag
     9241 gaacagccca aatgagtgac atgaataatc cggccgatga caacaacggc gcaatggacg
     9301 atctgtgggc tgaagcgttg agcgagcaaa aatcaaccag cgaaaaaagc gctgccgacg
     9361 cggtgttcca gcaatttggc ggtggtgatg tcagcggaac gttgcaggat atcgacctga
     9421 ttatggatat tccggtcaag ctgaccgtcg agctgggccg tacgcggatg accatcaaag
     9481 agctgttgcg tctgacgcaa gggtcagtcg tggcgctgga cggtctggcg ggcgaaccgc
     9541 tggatattct gaacaacggt tatttaatcg cccaaggcga agtggtggtt gttgccgata
     9601 aatatggcgt gcggatcacc gatatcatta ccccgtccga gcggatgcgc cgcctgagcc
     9661 gttagtgatg aataaccatg ctactgtgca atcttccgcg ccggtttctg ctgagccact
     9721 gctgcaggtg agcggcgcac tcatcgccat tattgccctg atcctcgctg ctgcctggct
     9781 ggtaaaacgg ctgggatttg cccctaaacg cactggcgtt aacggtctga aaattagcgc
     9841 cagtgcttcg ctgggcgcgc gtgaacgggt cgtggtggtc gatgtggaag atgcacggct
     9901 ggtgctcggc gttaccgcag ggcaaatcaa tctgctgcat aaacttcccc cttcagcacc
     9961 aacggaagag ataccgcaga ccgattttca gtcggtcatg aaaaatttgc ttaagcgtgg
    10021 cgggagatcc tgatgcgtcg tttgttttct gtcgcaccgg tccttctctg gctgattatg
    10081 cccctcgcct tcgcgcaact gccgggtatc accagccagc cgctacctgg cggtggacaa
    10141 agctggtcgc tcccggtgca gacgctggtg ttcatcacct cgttgacgtt cattccagcg
    10201 attttactga tgatgaccag tttcacccgc atcattattg tttttggttt attgcgtaac
    10261 gcgctgggaa cgccctccgc gccacctaac caggtattgc tggggctggc actgtttttg
    10321 acctttttta ttatgtcgcc agtgatcgac aaaatttatg tcgatgcgta ccagccattc
    10381 agcgaagaga aaatctcaat gcaggaggcg ctggaaaaag gggcgcagcc gctgcgtgag
    10441 tttatgctgc gtcagacccg cgaggcagat ttagggttgt ttgccagact ggcgaatacc
    10501 ggcccgttgc aggggcctga agctgtaccg atgcgcattt tgctcccggc ctacgtgacc
    10561 agcgagttga aaaccgcatt tcagataggc ttcacgattt tcatcccttt tttgattatc
    10621 gacctggtga ttgccagcgt gttgatggca ttggggatga tgatggttcc ccccgccacc
    10681 attgctctgc cctttaaact gatgctgttt gtactggtgg atggctggca attgctggtc
    10741 ggttcgctgg cgcagagctt ttacagctag agaggcaaaa tgacacctga atcggtcatg
    10801 atgatgggaa ctgaagcgat gaaagtcgcg ctggcactgg cagccccgct gttgctggta
    10861 gcgctggtca cgggccttat catcagtatt ttgcaggcag ccacgcagat taacgaaatg
    10921 acgctgtcgt ttattccgaa aatcatcgcc gtatttatcg ccatcattat tgccggaccg
    10981 tggatgctca atctgttgct ggattacgtc cgcaccttgt tcactaacct gccgtatatc
    11041 atcgggtagc ccgtacaatg atgcaggaga caagcgatca atggctttcc tggttaagcc
    11101 tgtacttctg gccgttactg cgcgtgctgg cgctcatttc taccgcgcca attctgagcg
    11161 aacgcagcgt gccgaaacgg gtaaaactgg gtctggcaat gatgatcacg ttcgccattg
    11221 ccccatcatt acctgccaac gatgttcctg ttttttcgtt ctttgctctg tggctggccg
    11281 tgcagcagat cctgatcggc attgcgcttg gttttaccat gcaatttgcc tttgccgctg
    11341 tgcgaaccgc tggcgaaatt atcggtctgc aaatggggct gtcatttgcg acatttaacg
    11401 gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg attggtagcg
    11461 aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg attttcctta
    11521 acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat ctggcattag
    11581 gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt ccattaactc
    11641 tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct ttttgcgaac
    11701 atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta ccattaatat
    11761 aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata tctgcgaaca
    11821 tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat atcgcaacat
    11881 ttactttaag acaattccag gcaaattata caacacttta cgggatagta agtccgcctg
    11941 aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc atgatgaaat
    12001 attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt caacgattat
    12061 tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga gtagaggggt
    12121 taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag cttgtgattc
    12181 acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg cttctaacag
    12241 tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg tttttatggc
    12301 aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat tgatcagttc
    12361 taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga aaaaagagac
    12421 aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg aatcgagtat
    12481 gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa tgaatatcaa
    12541 agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa cgcataataa
    12601 acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta tttttgtcaa
    12661 catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata agattactct
    12721 gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca gatgccgagc
    12781 gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc aactgccagt
    12841 accactccag gacgacgcgg accgctatcc gttttgactg ttacccgatc attcaccttc
    12901 atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc
    12961 aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc aggcggactt
    13021 aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag
    13081 tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga
    13141 tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa cacgttagcg
    13201 tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa ctggatattc
    13261 atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga
    13321 gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt acaccaggaa
    13381 atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa tattcgcgct
    13441 atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct tggtggctct
    13501 ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg ttttgagaat
    13561 catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca ctactggttt
    13621 ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa ccggcagccc
    13681 cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc agtaaaactt
    13741 cagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg ctgattgcag
    13801 agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt tttccacgca
    13861 tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta cgcgagaaag
    13921 aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc gaatggacgg
    13981 gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta acgctaatct
    14041 ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa ctgggcttgc
    14101 agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga aaagatcagg
    14161 ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca accacacttg
    14221 gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac gcggtgattg
    14281 tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc cgcgtctggc
    14341 gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt ttctccgccc
    14401 gttaagcgtg atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg aggtaaagcc
    14461 gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc tcgctgctac
    14521 ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc atctcttttt
    14581 catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga ttcgcgcctg
    14641 gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg
    14701 aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc
    14761 ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa ggatgttggt
    14821 gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca cggttatata
    14881 aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga acatacatgt
    14941 tgctgaccat ccggcgaatc acataccagg aaaggagtaa catggtggta aagagcgccc
    15001 acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg
    15061 tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta acatagcgac
    15121 tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc aaggccagtt
    15181 cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc aaaaatatca
    15241 gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg tttcttaaaa
    15301 attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag tagttattac
    15361 tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg
    15421 aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg ataaaccagg
    15481 gttgggtgac atagccgtaa taacgcgttg gtacattgcg cataaacaag gtcggctgcg
    15541 tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc gacgaggagt
    15601 tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc
    15661 tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat acgccattga
    15721 ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg cgatgctgtt
    15781 cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg agagcttcgc
    15841 gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg
    15901 cgttggctac gttttccaga tgattacgct ggctggagat ataggcatct tccagcacca
    15961 ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag cagagattaa
    16021 cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg ttttccattt
    16081 ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct ggcataaccc
    16141 ggcaatag
//
LOCUS       DADLTF010000088        16040 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.088, whole genome shotgun sequence.
ACCESSION   DADLTF010000088 DADLTF010000000
VERSION     DADLTF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16040
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.088"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1079
                     /locus_tag="JN749_002644"
     CDS             <1..1079
                     /locus_tag="JN749_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HAY7369011.1"
                     /translation="ECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPI
                     EAIIEARNKGGYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGD
                     ALKAADQHAKAEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLT
                     GHPINQYLKEIERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLD
                     DRSGRLEVMLFTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEARE
                     KYARGLAISLTDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRV
                     SPSDRLLNDLRGLIGSEQVELEFD"
     gene            1092..2051
                     /gene="accA"
                     /locus_tag="JN749_002645"
     CDS             1092..2051
                     /gene="accA"
                     /locus_tag="JN749_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAY7369012.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            2150..4291
                     /gene="ldcC"
                     /locus_tag="JN749_002646"
     CDS             2150..4291
                     /gene="ldcC"
                     /locus_tag="JN749_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HAY7369013.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            4348..4737
                     /locus_tag="JN749_002647"
     CDS             4348..4737
                     /locus_tag="JN749_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7369014.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            4802..6100
                     /gene="tilS"
                     /locus_tag="JN749_002648"
     CDS             4802..6100
                     /gene="tilS"
                     /locus_tag="JN749_002648"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAY7369015.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(6149..6409)
                     /gene="rof"
                     /locus_tag="JN749_002649"
     CDS             complement(6149..6409)
                     /gene="rof"
                     /locus_tag="JN749_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HAY7369016.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(6396..6596)
                     /locus_tag="JN749_002650"
     CDS             complement(6396..6596)
                     /locus_tag="JN749_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HAY7369017.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            6762..7295
                     /locus_tag="JN749_002651"
     CDS             6762..7295
                     /locus_tag="JN749_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HAY7369018.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAW"
     gene            7304..7726
                     /gene="arfB"
                     /locus_tag="JN749_002652"
     CDS             7304..7726
                     /gene="arfB"
                     /locus_tag="JN749_002652"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7369019.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            7740..8450
                     /gene="nlpE"
                     /locus_tag="JN749_002653"
                     /gene_synonym="cutF"
     CDS             7740..8450
                     /gene="nlpE"
                     /locus_tag="JN749_002653"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HAY7369020.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(8605..9429)
                     /locus_tag="JN749_002654"
     CDS             complement(8605..9429)
                     /locus_tag="JN749_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HAY7369021.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRNIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(9482..11200)
                     /gene="proS"
                     /locus_tag="JN749_002655"
     CDS             complement(9482..11200)
                     /gene="proS"
                     /locus_tag="JN749_002655"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAY7369022.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(11311..12018)
                     /gene="tsaA"
                     /locus_tag="JN749_002656"
     CDS             complement(11311..12018)
                     /gene="tsaA"
                     /locus_tag="JN749_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HAY7369023.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(12015..12419)
                     /gene="rcsF"
                     /locus_tag="JN749_002657"
     CDS             complement(12015..12419)
                     /gene="rcsF"
                     /locus_tag="JN749_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HAY7369024.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(12537..13352)
                     /gene="metQ"
                     /locus_tag="JN749_002658"
     CDS             complement(12537..13352)
                     /gene="metQ"
                     /locus_tag="JN749_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HAY7369025.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(13392..14045)
                     /gene="metI"
                     /locus_tag="JN749_002659"
     CDS             complement(13392..14045)
                     /gene="metI"
                     /locus_tag="JN749_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HAY7369026.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(14038..15069)
                     /gene="metN"
                     /locus_tag="JN749_002660"
     CDS             complement(14038..15069)
                     /gene="metN"
                     /locus_tag="JN749_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HAY7369027.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            15257..15832
                     /gene="gmhB"
                     /locus_tag="JN749_002661"
     CDS             15257..15832
                     /gene="gmhB"
                     /locus_tag="JN749_002661"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAY7369028.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3902 a         4030 c         4297 g         3811 t
ORIGIN      
        1 atgagtgctg gcggatgggg ctgaaaatcc tgccaccaga tataaactcc ggtctttacc
       61 atttccacgt caacgacgac ggcgaaatcg tgtatggtat tggcgccatc aaaggggtag
      121 gtgaaggtcc gattgaggcc atcatcgaag cccgtaataa aggcggctac ttccgcgaac
      181 tgtttgatct ctgcgcccgt accgacacca aaaagttaaa ccggcgggtg ctggaaaaac
      241 tgatcatgtc cggggcgttt gaccgtcttg ggccacaccg cgcggcgctg atgaactcgc
      301 tgggtgatgc gttaaaagcg gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg
      361 atatgttcgg cgtgctggcc gaagagccgg aacaaattga acaatcctac gccagctgcc
      421 aaccgtggcc ggagcaggtg gtattagatg gggaacgtga aacgttaggc ctgtacctga
      481 ccggacaccc tatcaaccag tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc
      541 tgaaagacat gcacccgaca gaacgtggta aagtcatcac agctgcgggg ctcgttgttg
      601 ccgcgcgggt tatggtcacc aagcgcggca atcgtatcgg tatctgcacg ctggatgacc
      661 gttccgggcg gctggaagtg atgttgttta ctgacgccct ggataaatac cagcaattgc
      721 tggaaaaaga ccgcatactt atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg
      781 ggcttaaaat gaccgctcgc gaagtgatgg atattgacga agcccgggaa aaatatgctc
      841 gcgggcttgc tatctcgctg acggacaggc aaattgatga ccagctttta aaccgactcc
      901 gtcagtctct ggaaccccac cgctctggga caattccagt acatctctac tatcagaggg
      961 cggatgcacg cgcgcggttg cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt
     1021 tattaaacga tctccgtggc ctcattggtt cggagcaggt ggaactggag tttgactaat
     1081 acaggaatac tatgagtctg aatttccttg attttgaaca gccgattgca gagctggaag
     1141 cgaaaatcga ttctctgact gcggttagcc gtcaggatga gaaactggat attaacatcg
     1201 atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact gacgcgtaaa atcttcgccg
     1261 atctcggtgc atggcagatt gcgcaactgg cacgccatcc gcagcgtcct tataccctag
     1321 attacgttcg cctggcattt gatgaatttg acgaactggc tggcgaccgt gcgtatgcag
     1381 acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg tcgtccggtg atgatcattg
     1441 gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg taactttggt atgccagcgc
     1501 cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta
     1561 tcatcacctt tatcgacacc ccgggggctt atcctggcgt gggcgcagaa gagcgtggtc
     1621 agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg ccttggcgta ccggtagttt
     1681 gtacggttat cggtgaaggt ggttctggcg gtgcgctggc gattggcgtg ggcgataaag
     1741 tgaatatgct gcaatacagc acctattccg ttatctcgcc ggaaggttgt gcgtccattc
     1801 tgtggaagag cgccgacaaa gcgccgctgg cggctgaagc gatgggtatc atcgctccgc
     1861 gtctgaaaga gctgaaactg atcgactcca tcatcccgga accgctgggc ggcgctcacc
     1921 gtaacccgga agcgatggcg gcatcgttga aagcgcaact gctggcggat ctggccgatc
     1981 tcgatgtgtt aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg
     2041 gttacgcgta attcgcaaaa gttctgaaaa agggtcactt cggtggccct tttttatcgc
     2101 cacggtttga gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat
     2161 tgccattatg ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc
     2221 ggcgctggtg gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa
     2281 atttatcgaa cataaccctc gaatttgcgg cgtgattttt gactgggatg agtacagtct
     2341 cgatttatgc agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa
     2401 cacccactcg acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga
     2461 atatgcgctg gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccaacgaata
     2521 tcttgataac attacgccgc cgttcacgaa agccttgttt acctacgtca aagagcggaa
     2581 gtacaccttt tgtacgccgg ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg
     2641 ctgtctgttt tatgattttt tcggcgggaa tacccttaag gctgatgtct ctatttcggt
     2701 caccgagctt ggttcgttgc ttgaccacac cgggccacac ctggaagcgg aagagtacat
     2761 cgcgcggact tttggcgcgg aacagagtta tatcgttacc aacgggacat cgacgtcgaa
     2821 caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaactg
     2881 ccataaatca ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc
     2941 gacgcgtaat gcgttgggga ttctgggggg gatcccgcgc cgtgaattta ctcgcgacag
     3001 catcgaagag aaagtcgctg ccactacgca agcacaatgg ccggttcatg cggtgatcac
     3061 caactccacc tatgatggtt tgctctacaa caccgactgg atcaaacaga cgctggatgt
     3121 cccgtcgatc cacttcgatt ccgcctgggt gccgtatact cattttcatc caatctacca
     3181 gggtaaaagt ggtatgagcg gcgagcgtgt tgcgggaaaa gttttcttcg aaacgcaatc
     3241 gacccacaaa atgctggcgg cgttatcgca ggcttcactg atccacatta aaggcgagta
     3301 tgacgaagag gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc
     3361 cattgttgct tcggttgaga cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct
     3421 gattaaccgt tcggtggagc gggcgctgca ttttcgcaaa gaggtccagc ggctgcggga
     3481 agagtctgac ggttggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg
     3541 ctggcccgtt gcgcctggcg aacaatggca cggctttaac gatgcggatg ccgatcatat
     3601 gtttctcgat ccggttaaag tcactatttt gacaccgggg atggacgaac agggcaatat
     3661 gagtgaggag gggatcccgg cggcgctggt agcaaaattc ctcgacgaac gtgggatcgt
     3721 agtagagaaa accggccctt ataacctgct gtttctcttt agtattggca tcgataaaac
     3781 caaagcaatg ggattattgc gtgggttgac ggaatttaag cgctcttacg atctcaacct
     3841 gcggatcaaa aatatgctgc ccgatctcta tgctgaagat cccgatttct accgcaatat
     3901 gcgtattcag gatctggcgc aagggatcca taagctgatt cgtaaacacg atctttccgg
     3961 tttgatgttg cgggcattcg atactttgcc ggaaatgatc atgacgccac atcaggcatg
     4021 gcagagacag attaaaggtg aagtagaaac cattgcgctg gaacaactgg tcggtagagt
     4081 atcggcaaat atgatcctgc cttatccacc gggcgtaccg ctgttgatgc caggagaaat
     4141 gctgaccaaa gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagggca
     4201 acattacccc ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggcgttta
     4261 ccgcgtacga gtcctaaaaa tggcgggata acttgccaga gcggcttctg ggcgagtaac
     4321 gtgctgttaa caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg
     4381 attattgcga cggattatgc ggtgagcaaa gctttctact gcgatattct tggtttcacg
     4441 ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat
     4501 gggcaatatg tgattgagct tttctctttt ccgttcccgc cggagcgccc cagccgaccg
     4561 gaagcttgcg gcctgcgtca tctggctttt agcgttgatg acatcgatgc ggcagtggcg
     4621 caccttgaaa gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa
     4681 cgcttcacct tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc
     4741 ttgtcatcgc cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat
     4801 catgacactc acgctcaata gaccacttct cacctcacgc cagattctgg tggcctttag
     4861 cggcgggctt gactccaccg ttctgctgca tcagttggtg cagtggcgga cggaaaatcc
     4921 gggcgtcact ctgcgcgcta ttcatgtgca tcacggttta agtgccaatg ccgatgcctg
     4981 ggttacgcat tgcgaaaacg tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt
     5041 acaacttgcg caagaggggc tggggattga ggcccaggcg cggcaggcgc gttatcaggc
     5101 atttgcccgt accctattgc ccggcgaagt gctggtcacc gcgcaacatc tcgacgatca
     5161 atgtgaaacc tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat
     5221 ggcggaagtt tcggagtttg ctggaacacg gcttatccgc ccgttgcttg cccgcacgcg
     5281 gggggaactg gagcagtggg cattggcgca tggtttacgc tggattgaag acgaaagtaa
     5341 tcaggacgac tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca
     5401 gcgttggccg cattttgcgg aagcaacggc ccgcagcgcc acactttgtg ctgaacaaga
     5461 gagtctgttg gatgaactgc tggcggatga tttagcacac tgccaaacgt cgcaggggac
     5521 gctgcagatt gcgccaatgc tggcgatgag tgatgcccgt cgcgcggcga ttattcgccg
     5581 ctggctggca gggcagaatg caccgatgcc ttcccgcgac gcgctggtgc gcatctggca
     5641 ggaagtggcg ctggcgcggg aagatgcttc cccctgttta cgtttgggcg cctttgaaat
     5701 ccgccgctat cagtcgcaac tgtggtggat taaatccgtc accgggcaaa gcgaaaccat
     5761 tgtgctgtgg cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaaccgtaca
     5821 gcttactgcg ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt
     5881 caaagcgcca ggattgctgc atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat
     5941 ctggcaagag ttaggcgtgc cgccgtggct acgtgacacc acgccactgc tgttttatgg
     6001 agaaacgctg attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga
     6061 gaatggcgta agttttgtct ggcagaaaac gcttagttaa gtgaaagccg gataagacgc
     6121 atcaaacgtc gcatccggcg aaagtcaatc aggattcgct taccaccacc gtaccgattt
     6181 cagggtggct aaagctggta attttatcca gacgcagctc gcgggtttcg ccagcggcct
     6241 cgacgaccag atactccaca tttttgcggg agactaaatc actggctttt gcctgcaatt
     6301 tttcgccatc tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat
     6361 tatcgtaatc atcacaatta attggttgat acgtatcatt cattgacata atcgctcacc
     6421 agtaagtttg ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg
     6481 gcagccattt cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag
     6541 tctccgctgg cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc
     6601 cctcctgtca gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc
     6661 tgtttcgcaa ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt
     6721 attccgttac aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa
     6781 tttataaagc gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc
     6841 tgacgctggc gcgccaccct tcagaaaccc aggagcgtat gatgctgcgc ttgctggcgt
     6901 ggctgaaata tgctgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc
     6961 cggaagcgtg gctgcgtaac gatcacctgg gcattgattt gtggattgag ctggggctgc
     7021 cggatgagcg acgaattaag aaagcctgca cccaggccgc agaagtggcg ctgtttacct
     7081 ataatagtcg ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gtgcagtttg
     7141 ccaatctttc cgtctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg
     7201 atcgtaccat gacgctgcag gcaacgattc aggatggtgt tatctggtta tcggatgata
     7261 agaataatct ggaagttaac ttaaccgcct ggtaacaacc ttcatgattg tgatttcccg
     7321 acatgttgct attcccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgctgg
     7381 cgggcagcat gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgcc
     7441 cagcctgcca gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag
     7501 tgatggcgtg attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc
     7561 cgctctggcc cggttggtgg cagtgattaa agatttaaca aaagaacaaa aagcccgacg
     7621 acccacgcgg cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc
     7681 aagcgtgaag gcgatgcgcg gcaaagtacg cagcggtcgg gaataaaaag aaggaatgga
     7741 tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat
     7801 gtaataatcg ggccgaagtc gatacgcttt ctccggcgca ggctgccgaa ctgaaaccga
     7861 tgccgcaaag ttggcgcggc gtgctaccgt gtgccgattg cgaaggaatc gaaatctctc
     7921 tgttcctcga aaaagacgga acatgggtga tgaatgagcg ttatctcggt gctcgtgaag
     7981 aaccttcctc cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa
     8041 ccgacagcaa aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg
     8101 atcgtgaagg caatccaatt gaatcgcagt tcaactatac gctggaaccg gcacaatcca
     8161 gtttacctat gacgccgatg accctgcgag gcatgtattt ttatatggct gatgcggcga
     8221 ccttcactga ttgcgcgacc ggaaaacgtt tcatggtagc gaataacgca gagctggagc
     8281 gtagctacct ggctgcgcgc ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc
     8341 actttacgct tgaggctaat ccggatactg gtgcgccaac caaagtatta gcgcccgata
     8401 cggcgggtaa attttacccg aataaggatt gcagtagttt ggggcagtaa acctgcttaa
     8461 ggcagaaaca aacgcaaaat tgcctgatgc gctgcgctta tcaggcctac agcatataat
     8521 gcaacatttt gaattagcac gattttgtag gccggataag gcgtttacgc cgcatccggc
     8581 attttgtgca cgaggctggc tttatcactg agtcagccca acaaagcgac ccgccttgat
     8641 ataaatccca ggcttcaggt gcccaacata ctgtaactgc gtttctggct taaacgcaga
     8701 gacatcacca atacgcatat gcatcagatc ggcagggcta atccagcccg attgcgccag
     8761 tgtaagtgga tgcccggctt tggcaaatgc atcagtgaca aattccgaac aaaaccacga
     8821 ctttttgtct ccttcgcaca cactgcttaa ctgcgctttc gccaggtcgc tgacgcactg
     8881 ttggcgaaaa tcctcggaga acggattcag tgagcacatc tggcgagtca ccataaaggg
     8941 aataaattcg acaatgttgc gatagttata accgctatct ttgattttat tggcaaacgc
     9001 ggtgatatct gtggcttgtt gcggggtaag atccgggact cgtaagacga aaagcttatc
     9061 actatgcttc atcgcttttt taagggaaac aatctggacg ccagcgcctg tcgcttctgc
     9121 aacgttatta tcacccagaa agattgcaac gtgactcaca gaggaagtgc tgaagacgcg
     9181 gattccaaat gaggttaccc caaggcttga ggagaacagc aaatcgccgg gtttgagatc
     9241 aggtgccgtt atttctttta ttgattgttc ggtgaaagag ctttgatgct ggaatttgac
     9301 agcccatgtt tttgcctcgg catctactgc tgtggctgat gggtctggct ggctgatatc
     9361 aacggtacaa gccgaaagta aaagaaagct ggggagaagc aggcggcagt acgcctttgg
     9421 tttatccatt ttatacaatc catgtaaaaa agggccctga aattcaggac cctttctggc
     9481 atcagccttt aatctgtttc accagatatt cgacgatgtc accagtctta attaactgtt
     9541 tctcgccgtt gcgacgatat ttatattcga tatcgtcgtt gtcgaggtta cggtcgccca
     9601 gcacaatagt gtgcggaata ccgatcagtt ccatatcagc aaacatcacg cccggacgct
     9661 ctttgcggtc atccagcagc acttcgatac cttgtgcacg cagttcgctg tacagtttct
     9721 cagcaagctc ttgtacgcgg aaggatttgt gcatgttcat cggcagaatc gccacctgga
     9781 acggcgcgat agcgtcaggc catacgatgc cgcgttcgtc gtagttctgc tcaatcgccg
     9841 cagctaccac acgcgttacc ccgataccgt agcaacccat cgtcaggatt tggttacggc
     9901 catcttcacc ctgtacggag gctttcagtg cttcggagta cttggtaccc agctggaaga
     9961 tgtgaccaac ttcgatacca cgtttgatca gcagcgtacc ctggccatcc gggcttggat
    10021 cgccagccac cacgttacgg atatctgcaa cttccggggt agcgacatcg cgatcccagt
    10081 tgataccgaa gtagtgttta ccatcgatgt tagcaccagc agcgaaatca ctcatcgccg
    10141 caacggtacg gtcaatcacc accggaatcg gcatgtttac cggacccagt gaacccggac
    10201 cggctttaac cacagcacga atttcttctt cggtcgcgaa agtcagcggg cttgcaacct
    10261 gcggcagttt ttctgcttta acttcgttca gctcgtgatc accgcgcacc agcagcgcaa
    10321 ccagcgggaa gctactgcct tcaaccgctt taaccagcag agtcttaacc gttttctcaa
    10381 tcggcagatt gaactgttca accagttccg cgatggtttt cgcgttcggc gtatcaacca
    10441 gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg cgcgatagct tctgccagtt
    10501 caatgttcgc tgcatagtca gaggtgtcgg agaagaccac atcgtcttca ccgctctgcg
    10561 ccagcacctg gaattcgtga gaggcgctgc cgccgataga accggtgtcg gcctgtacgg
    10621 cgcggaaatc cagccccatg cggctgaaga ttttgctgta ggccgcatac attgcatcgt
    10681 aggtttcctg cagggattcc tgagaagtat ggaaagagta agcatctttc atcaggaatt
    10741 cgcgggaacg catgacgccg aaacgcggac gcacttcgtc gcggaacttg gtctggatct
    10801 gatagaagtt cagcggcagc tgtttgtaag agctaagctc gttacgaatc aggtcagtga
    10861 taacttcttc atgagttggg ccgagtacga acggacgctc gccacggtca acaaaacgca
    10921 gcagttccgg accgtactgt tcccaacgac cactctcttg ccacaaatcg gctggctgaa
    10981 ccaccggcat cgacacctcg atcgcaccgg cgttgttcat ctcttcacgc acgatgtttt
    11041 cgactttttt cagaacgcgc acgccggtcg gcagccaggt atataacccg gaggccagct
    11101 tgcggatcat cccggcgcgc agcatcagct gatggctgat cacctcggcg tcggcaggtg
    11161 tctccttgag agtggagagc aggtattggc tagtacgcat gttgttacgg ttccagttgg
    11221 aaggtagaac aggctcaagg cgagcctggg acaaaaaaag tgatttagtt taccagtgca
    11281 aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt tccagcgcaa agacttcaaa
    11341 acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgt agccagacgg cataggtttt
    11401 gcccgtttcc tcacctttac gataggccgg gcgcgggtcc tgcgccagta cttcgcggat
    11461 aaacagcgtt aactgcggat aacgcttctc cagcgtcaaa agctgctttt cgacctctgc
    11521 ggtaaaactc accgccatct ctgcagctgg cgcactttgc gcatagctgg cactggcatc
    11581 gggaagcgat tcggcaaagg ggagatacgg tttgatatcc actaccggcg taccatcgac
    11641 cagatccaga ctgccgagct tcagaatcac gctgtcttta tggcaaacaa cctctttcag
    11701 ctctaccagc gacatgccaa ttgggttagg gcggaaagta gagcgtgttg cgaaaacccc
    11761 cattctggcg ttaccgccga ggcgcggtgg acgcacagtc ggacgccagc cgccttccat
    11821 cgtttgatga aagacgaaaa ggatccataa atggctgaat gcttccaggc cgcgaacggc
    11881 gtcggcctgg ttgtaggaag caatgagatg cagttcgccg ttggcgcttt ttaccagacc
    11941 tggctggcgc ggaacggcga atttttcttt atagggcgag cgaataacgc ctatttgctc
    12001 gaactggaaa ctgctcattt cgccgtaatg ttaagcgcag agccgataca tacagcctga
    12061 cgatagcagc ccggcgtacc gctggtgact tcgcagctat gcagtaatac agcattggct
    12121 ttcattttag aggcattgat ttgcatctgc ttacgtgcgg ttggaatgct cggcggagag
    12181 tcctgattag aggcctggca agagtcgcca ctgacttcac cgagatcgcg gaacggtttg
    12241 ccgactaatt cttctgcatt ggtataaatt cggaccggcg tggcgcgcgg cgctttcggt
    12301 tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa cgggttcgac aggggatctg
    12361 cttaacatgg aacagccgct tagcatgagt gctactaaac agatcggtaa agcacgcata
    12421 gtatttcctc aatgtatgat caaaacgtca atattgaatc aggagcttgt aaaaatgaca
    12481 agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt ggaaacagcc taaaaattac
    12541 cagcctttaa cagcgccgcc gttaaacact ttgtttgctg cttcgtaaac ttcatcagac
    12601 tgataagcct ggacgaattt cttcacgttc tcggcgtctt tgttatcttc acgcgttacg
    12661 atcaggttta cgtatgggga gtctttatct tcgacaaaga taccgtcttt cgccggagtc
    12721 aggccaatct ggctggcata ggtggtattg ataactgcca gagcgatttg cgcgtcgtcc
    12781 agagaacgcg gcagttgcgg tgcttccagt tcaacaattt tcagattttt tgggttctca
    12841 acaacatcaa gaactgtcgg cagcaggcca acgccatctt tcagtttgat caagcccact
    12901 ttttgcagca gcagcagtga acgaccaaga ttagttggat cgtttggcac ggcaacctgc
    12961 gaaccatcct gcagttcatc cagtgatttg attttcttgg agtaaccagc aatcggataa
    13021 acaaaagtgt tgcctactgc gaccagtttg tagccacgat ctttcagttg ctgatcaagg
    13081 tacggtttat gctggaaggc gttggcgtcg atatcgcctt tgctcaatgc ttcgtttggc
    13141 agaacatagt cgttgaaggt taccagctca acgtccaggc catatttgtc tttcgcaact
    13201 ttctgcgcga cttctgctac ctgctgttcg gcaccaacaa tcacgccgac tttaatgtgg
    13261 tttggatctt tttcatcctg accgcagcct accagtgcca gtgatccaat cagggctccc
    13321 actgccgcaa aggttttgaa tttgaacgcc ataccttatt ccttcttcaa ttatttatgt
    13381 tgtgttgaac gttacttgcg agtgacagcc cggacgatgc ggtcgcctgc gaactgaatt
    13441 aaataaacca gaatgaccag caataccagt accgtattca tcaccgtcgc gttgtagccg
    13501 atgtagccat actgatagcc aatctgacct aaaccaccgg caccgactgc accacccatc
    13561 gcggaataac cgaccagggt aatcagggtg atagttgccg cattcaccag acccggcaac
    13621 gcttccggta acagcacttt acggacgatc tgcattggcg tggcacccat tgcgcgggaa
    13681 gcttcaatta acccggttgg gatctccagc agagcgttct cgaccatacg ggcaataaac
    13741 ggtgctgcac caacggttaa cggaacaatt gctgcctgca aaccaatcga tgtaccgaca
    13801 ataacgcggg taaacggaat catccatacc agcaagataa tgaacgggat ggaacggaaa
    13861 atgttcacaa tcgcagaaac ggtacgatac agcttcgcgt tagcaataat ttgccccgga
    13921 cgcgtgacat aaagcagaac gccaaccggc agaccaatca caaacccaaa aaatccggat
    13981 acgaaggtca ttgccagcgt ttcccatacg ccacgaacca gcagccacat catcggctca
    14041 gacataaccc agtacctcta cttttacatg gtgttcctgc agccaggcaa tggcggcttg
    14101 cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg ccgaatttaa cgccaccggc
    14161 gtagtccatc tgcgcgctaa taatgttgtt gttgacgttg aaacgacgcg cggtttcaga
    14221 aagcagtggg gcatcgaccg attgaccggt aaactccaga cgcagcatcg gcacgcagtc
    14281 agtaaatggc tccgtttgca gacgttcctg gtaatcttcc gggatatcca gatgcagggt
    14341 cgactgaata aacttctgcg ccagcggcgt tttcggatgc gagaacactt cacttaccgt
    14401 gtcctgctcg atcagttcgc cattgctgat gaccgccacg caatcacaaa tacgcttcac
    14461 aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc agacggcggt taatgtcttt
    14521 cagcagttcg agaatagaac gtgtcgttgc cgggtccagc gcgctggtgg cttcatcaca
    14581 cagcaatact ttgggattgc tggctaatgc acgggcaatc gccacacgtt gtttctgccc
    14641 accggaaaga ttcgacgggt agctatcatg cttatcgcca agaccaacca atgacagcaa
    14701 ttccgtcacg cgacgtttga tctcgtcttt cggtgtgttg tccagctcca gcggcagagc
    14761 cacgttgcca aaaacagtac gcgaagagag caggttaaaa tgctggaaaa tcataccaat
    14821 ctggcggcga gctttggtca actcggattc tgacagcgtg gtcaattcct ggccatcgac
    14881 cagcacgcta ccctcggttg ggcgctccag caggtttaca caacgtataa gcgtactctt
    14941 acccgcgcct gaggcaccga taacgccata aatttgtcca gctggcacat gcaggctgac
    15001 gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac actttggtga tattcgaaag
    15061 ttttatcatt gattatttat tatcgtcatt aagttagtcg tagcatctcg aatgcctgaa
    15121 acgggcaacg ccgtcaatga aatggatgtt aaggcatcca gacgtctaaa tcaatcaggt
    15181 ttatgcgaag agcactttct tgcaggtcga aacatgcgat actagcgtcc catgccttat
    15241 taaggagcta taaaaggtgg cgaagagcgt acccgcaatt tttcttgacc gtgatggcac
    15301 cattaatgtc gatcacggct atgtccatga gatcgacaac tttgaattta tcgacggtgt
    15361 tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg ctggtggtag taaccaacca
    15421 gtctggcatt gctcgcggta aatttaccga agcacagttt gaaacgctga ccgagtggat
    15481 ggactggtcg ctggcggacc gagatgtcga tctggatggt atctattatt gcccgcatca
    15541 tccgcagggg agtgttgaag agtttcgcca ggtctgcgat tgccgcaaac cacatccggg
    15601 gatgcttttg tcagcacgcg attatttgca tattgatatg gccgcttctt atatggtggg
    15661 cgataaatta gaagatatgc aggcagcggt tgcggcgaac gtgggaacaa aagtgctggt
    15721 gcgtacgggt aaacctatta cacctgaagc agaaaacgcg gcagattggg tgttaaatag
    15781 cctggcagac ctgccgcaag cgataaaaaa gcagcaaaaa ccggcgcaat gattaaaaga
    15841 tgaacggttg aaataaaaat gcatttttcc gcttgtcttc ctgagccgac tccctataat
    15901 gcgcctccat cgacacggcg gatgtgaatc acttcacaca aacagccggt tcggttgaag
    15961 agaaaaaatc ctgaaattca ggggtgactc tgaaagagga aagtgtgata tacgccacct
    16021 cgcgacagtg agctgaaagc
//
LOCUS       DADLTF010000089        15952 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.089, whole genome shotgun sequence.
ACCESSION   DADLTF010000089 DADLTF010000000
VERSION     DADLTF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.089"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JN749_002662"
     CDS             complement(16..1011)
                     /gene="yjfF"
                     /locus_tag="JN749_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HAY7369029.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(998..2020)
                     /locus_tag="JN749_002663"
     CDS             complement(998..2020)
                     /locus_tag="JN749_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7369030.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNLVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2034..3536)
                     /locus_tag="JN749_002664"
     CDS             complement(2034..3536)
                     /locus_tag="JN749_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAY7369031.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDMREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLNTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JN749_002665"
                     /pseudo
     CDS             complement(3676..4633)
                     /gene="ytfQ"
                     /locus_tag="JN749_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4943..5473
                     /gene="ppa"
                     /locus_tag="JN749_002666"
     CDS             4943..5473
                     /gene="ppa"
                     /locus_tag="JN749_002666"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HAY7369032.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5853..6194)
                     /locus_tag="JN749_002667"
     CDS             complement(5853..6194)
                     /locus_tag="JN749_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HAY7369033.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JN749_002668"
     CDS             complement(6197..9976)
                     /gene="tamB"
                     /locus_tag="JN749_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HAY7369034.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JN749_002669"
     CDS             complement(9973..11706)
                     /gene="tamA"
                     /locus_tag="JN749_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HAY7369035.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11912..12550
                     /gene="msrA"
                     /locus_tag="JN749_002670"
     CDS             11912..12550
                     /gene="msrA"
                     /locus_tag="JN749_002670"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAY7369036.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12873..14216
                     /locus_tag="JN749_002671"
     CDS             12873..14216
                     /locus_tag="JN749_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAY7369037.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14278..14484)
                     /locus_tag="JN749_002672"
     CDS             complement(14278..14484)
                     /locus_tag="JN749_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HAY7369038.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14809..15363
                     /locus_tag="JN749_002673"
     CDS             14809..15363
                     /locus_tag="JN749_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HAY7369039.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPRSQFIVDSNGVARGAWQLDDESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15426..>15952)
                     /locus_tag="JN749_002674"
     CDS             complement(15426..>15952)
                     /locus_tag="JN749_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369040.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3798 a         4373 c         3920 g         3861 t
ORIGIN      
        1 cacggagagg catttttatc gctgcgcaat gttgactctt gtcactggcg aactctgacg
       61 attctcccac agcaccgtta acccacgctg taatgctata aaaataaaca acaaaatacc
      121 gatggcgatt ttcgtccacc aggaactcag cgtgccgtca aagttgatgt aagtctggat
      181 cagtccctga attgccacgc caaaaagcgt cccaagcacc gtcccgacgc caccacttaa
      241 caacgtaccg ccaatcacca ccgacgcgat ggcgtccagt tccacaccta cgcccgccag
      301 tgcatatccg gcttgggtat aaatcgagaa gacaatcccc gccagtgttg ccagtccggt
      361 ggagagcata taaatgcgaa tagtggtgct gcgagtagaa atccccatca ggttcgccga
      421 cgtcgcgttg ccgccaatag cgtacacctg attgccaaaa cgggtacgat gcgcaaggaa
      481 aataccgata accaccaccg ccagcatcaa cagccccatc gcactaaggc gaccgccgcc
      541 ggggattttc cacgcaaggc ttgagagcgt gtcataaatg ggatggttta tcggaatcga
      601 ctcttccgaa acgagatagc tgacgccgcg caaaaagaac atccccgcaa gcgtaatgat
      661 aaatgccggg atcttcaggg cgtcgatcag aagccccata aatgcgccga aggcacagcc
      721 catcaccagc actagcggaa acgccagcag cggcgagagg ccgaaatcgc caaccacttt
      781 tgccagaaac acgccagtaa aggcgatcac cgaaccgacg gagagatcga taccgccaga
      841 gaggatcaca aaggtcatgc caacggcgat tatcccaaga aaggcgttat cggtcaggat
      901 attgcagatc acccttgtgg aagcaaaacc gggaaactgg gtcaggcagt agagatagcc
      961 cagcacaaag acgccgatgg tgatcatcag cggcaagtta cgttttatca tggctacgta
     1021 ctcctttaat cagactgata aagcgttgcg actgaacaat cagcacacaa agcaccacca
     1081 ccgctttcac caccaggttc atctccggcg gaaagcccga aagcaaaatt ccggtgttca
     1141 tcccctgaat aatcagcgcc cccactaccg agagcagcag gttaaagcgc ccgcccatca
     1201 gcgatccgcc gccaatcacc accgcgagaa tggcgtccag ctccagccat aatccggcgt
     1261 tgttggcatc ggcaccgcaa atatccgccg ccacgataat gcccgcaatc gccgcacata
     1321 gcccgctcaa cacgtaggtg agcatgacga tgattcgcgt gtttaccccg gcattttttg
     1381 ccgcccgaat gttgatacca acgacttcga taaacatccc cagcgccgtt ttgcgggtca
     1441 acagccagaa caggagaagc gtcagcaccg cgataatgac cggcgttggc aggaacaaca
     1501 gcgatccgct gccaaaccat gagagatccg gcgagttaaa cgtgacgatc tgcccggcgg
     1561 tgatcagttg cgccacgccg cgcccggcga ccatcaggat cagggtggcg acaaacggct
     1621 gaattttgag gatcgctacc agtatgccgt tccacaatcc cgccaggatg ccggtgccca
     1681 gggcgcttaa caaaacaatc ggcaggctga atcccgcgac cgtcatcgca gccgttgtgg
     1741 ctccggcgat agccatcacc gcccccacag agagatcgat accaccggtg gcgatcacca
     1801 gcgtcatacc aatcgccagt aacgcaacgg gagctgcacg gttaagaatg tcgatagggc
     1861 taccgaacaa acgcccatcc tgaagcacca cctgccagaa atgcggggct accaggctat
     1921 cgaccagcag caccagcaac agtgccgcca gctgcggcat ccccgtcggc cagtgaaaac
     1981 gcctttttgg cggcgtagtg tcagggagag attgaggcat cacactgttc tccttacgcc
     2041 gcaatggcgt tcatgatcgc cggaacggaa agctgtgcca gcgggatctc cgccacctgt
     2101 ttgcgatcgc gcatgatgat cacccggtcg gcatagccca ccagctcttc cagttcggag
     2161 gagatcacca gcagcgccag accgtcagca cacagcgttt caatcaggcg gatgatctcg
     2221 gcgtgggcac caacatcaat gccacgggtt agctcatcaa ggatcagaaa ttgcggtcgg
     2281 gtcagtagcc aacgtgaaag cagcactttt tgctgattgc caccggagag aaattcaatc
     2341 ggttgttcag ttgaaggtgt gcgaatgcca agctggcgga taaagcgttc ggcaatctct
     2401 tgctgctctt tgcgggaaat gggtcgtagc cagccgcgct gggcctggag agcaaggatg
     2461 atattttccc gcaccgaggc ggcggcgatg atgccatcgg ttttcctgtc ttccgggcag
     2521 aatccgatgc ccagtactga agcctgatgt ggcgatcgca gggtttgcgg tttgcctttg
     2581 atcaacgccg tgccgctgtc agcaggtttg ataccgaaga tcacttcggc ggtttcggta
     2641 cgtccggatc ccagcaagcc agccagaccg acgatctcgc cggggcgcac ttcgagatca
     2701 aacggtgcta tcgttccttt tttgccgtaa tttttgaacg cggcaacggg tttgtcgctc
     2761 aacaatgttc gcccggcacg ctgtagcgcg tgggtatcca gctcgcgccc cagcatcatt
     2821 tttaccagtt cgatctgcgg tagctcgcgc gtttcccgac agcctacgaa actgccgttg
     2881 cgtaagacgg tgatccgatc gctgacctga tagacctgat cgagaaagtg agtgacaaag
     2941 atcaggctga cgccgcgatc gcgcaactga cgcatcaggc caaataatag ctccacttct
     3001 tgggtgttga ggctggcggt gggttcatcg aggatcagca ctttggcgga gagatcgata
     3061 gcccggcaaa tggcgacgat ttgctgcatc gccaccgaaa aacggttaag cggttcgcgc
     3121 atgtcgaggg agaaaccgta agatgccatc agttcggtgg cgcgcttttc catctcttta
     3181 cggcgtagaa ggccgaaacg tttgggttcg cgacctatga atagattatc agcaaccgac
     3241 atattgggta gcaggttgac ttcctgatag acggtgccga taccgagttg ttgtgcatgg
     3301 gcggtatttt tcggtgagat agtctggcct tccagccaga tggtgccgcg atcggcgtgg
     3361 tatacaccag ttaatgcttt gattagcgtt gattttcccg ccccgttttc accaagcagc
     3421 gccatgattt cgccacggcg caggctgaaa tcaacgttgt ctaacgcttt gacgccgggg
     3481 aaaaatttac ttaatccttc ggtgcggagg atctcctggt gttggtcggt cgtcatggtt
     3541 tcccccggtg taaagcgtgc tcccagtagg catgataaga cgcggtaagc gtcgcatcag
     3601 gcttcagtgc accatcgccg gatgcggcgc gagcgcctta tccggcctac gaatcggcat
     3661 caggcatagc aaccctcaat accccatatt tttcttcttc tctaactctt cttttgcggt
     3721 atcaggcagg taaagggtgg atttggttaa cgtcagcttt tcaggcatgg tgccgtcttt
     3781 tttgtatttc tccagcgcat cgaaagcggg tcccgccatg ttcggcgtca gttcaacact
     3841 ggcgttcgct tcgccatcca tcatcgcttt gtagatgtcc ggtacaccgt caatggaacc
     3901 cgtgaggata tctttgcccg gtttcaggcc cgcttcttta attgcctgaa ttgcaccaat
     3961 caccatgtcg tcgttatggg cgtaaaccat gcagatgttt ttgccgttgt tttccgcttt
     4021 gataaagctc tccatgactt ctttgccttt actgcgggtg aagtcacctg actgcgagcg
     4081 gataattttg atatttggcg cattcttaat ggcttcggca aagcctttct taacggtcaa
     4141 tggcgacgct ggccccaacg gtgccctgca gctccaccac gttgcatggt ttgccattca
     4201 cttcttttac cagccagtca ccgatcaact taccttcgag gatgttgtcg gcagtaacgg
     4261 tggtcatata gagagatttg tctttcacat cgatggaacg gtcgagcaag aagaccggga
     4321 tttcggcatc tttcgcctct tttaataccg gttcccaacc cgtcgcaacc actggagcga
     4381 taaagatcgc atccacccct tgtgcaacga aggaacgtac cgctttaatc tggttttcct
     4441 gcttttgctg accatcggca attttcaacg taattccgcg tttttcggcc tcacttttcg
     4501 ccacattggt ttctgcggcg cgccagccgg attccgatct gacctgcgaa aatcctacgg
     4561 ttaatggagc ggccaacgcc atagacgaca tggctgccga gactgcagag actacaagta
     4621 agcgtttcca catagggttg tcctcgtcgg ggtaattatt attggtgaaa agatgttcgc
     4681 gaaaaaacta tagacaattc gttatgtaac ggattgcgtt acatcacact tcaaaaaagt
     4741 aaataaaacg ttaatcacaa gtttgtaatc gctttcatct cactatgaaa aatgcggcta
     4801 cggttatgga ttttcctgct ctgtataccg tcttaaaact ggcgaaaaag gaaaatgaag
     4861 acgaaaacaa gcaaagacat tcggcgcgag ttggctataa tacttggcac ttgtttgcca
     4921 catattttta aaggaaacag acatgagctt actcaacgtc cctgcgggta aagatctgcc
     4981 ggaagacatc tacgttgtta ttgagatccc ggctaacgca gatccgatca aatacgaaat
     5041 cgacaaagag agcggcgcac tgttcgttga ccgcttcatg tccaccgcga tgttctatcc
     5101 gtgcaactac ggttacatca accacaccct gtctctggac ggtgacccgg ttgacgtact
     5161 ggtcccgact ccgtacccgc tgcagcctgg ttctgtgatc cgttgccgtc cggttggcgt
     5221 tctgaaaatg accgacgaag ccggtgaaga tgcgaaactg gttgcggttc cgcacagcaa
     5281 gctgagcaaa gaatacgatc acattaaaga cgtaaacgat ctgcctgaac tgctgaaagc
     5341 gcaaatcgct cacttcttcg agcactacaa agacctcgaa aaaggcaagt gggtgaaagt
     5401 tgaaggttgg gaaaacgcag aagccgctaa agctgaaatc gttgcttcct tcgagcgcgc
     5461 aaagaataaa taagttcttc tagcgcaata accctgaacg ccgggcttcg gttagtaagg
     5521 gtttttttat gcccgcgata aataaactct ctattccacc atcattattc tcagcggttg
     5581 caaggcttga acggtaagaa caagcaaacc cgaccaccat tttgctgttc atagccactt
     5641 gctggaagtt agccgacctc actcatactc accgttcaaa gcgtgccccc gttttacccc
     5701 tgcacatttg tgaacgccag cagagttttt tatgcgattg cagatatgga aagcggctcg
     5761 gagagaaaag caaaagatgg aaaagttaca aaaaaaaatc gcgagataca tcgcataaaa
     5821 caacgccacc cgaaggaggc gtgcttttat tattacttat ccctgtctaa ccagtcgccg
     5881 ctttcaatta gctttaatcc atcgacgggt cgttgataaa cgtacatcca tgcactccca
     5941 tacggcgtct gaatcaactg gcgcgcgtat tcaccgcccc tggtgcgcaa ggcatcaagt
     6001 tcggccagcg tggcgttgtc aatacgataa acttcaccgt gtaccgttcc gttccccgga
     6061 actgcgcctg gatagtggcc caggctatac aactggtagt tatcgatact gaaatcgccc
     6121 agtaactggg cattggtcat ccagtgactg ttgccttgtt tgtggcgtaa actgccgtag
     6181 acaaatattc gcattgctaa aactcgaact gatagagcaa atccagtgcc tggtctacac
     6241 cagacacggc ttccagatat agcttaggca tcaggcgata acgtaacgtg agtgttgcta
     6301 tagagtcaaa tatacccacg ccgtatttca cttgcagacc tggcaataca tagccgctga
     6361 ccactacctg ggaggagtcg cctactccca gggtgtcgag cgctaaattg cttacgccaa
     6421 acgtctcgcc gattttaccc acaatctggc cactttgcgc aacccccaga ccaatcagca
     6481 tcgaggtcat tgccgcactg tcgctctgat cgctctccag cccttgtcca cgtagcaaat
     6541 aagacaatgc agcttgttgc gacatcgccg ggtcagagaa gatctccgct ttcggttcgt
     6601 ccgccagacc agtgacgcga actccggcga ttacatcgtc ttctgtagca tccgggttac
     6661 gaatagcttc aatattaaga tacggttgat ccggcggacc agagaacagt aactcaccct
     6721 tacgcacaat cagatcctga ccataggcat ggaagcgccc ttcagggatg ttgatctgcc
     6781 cgttcaggcc cagcccttgt ttgtcctgta cgacgttaag atcgcccgtc agccgcgctt
     6841 tcaggccaaa ggcgtcaatg cgcacattgt tgccaacgtg gacaatcagg ttactgttaa
     6901 tcggaatcga cgccgttttc ggctcttccg gttgcaggtt atcgttaagc atcaccacat
     6961 cgctggagac gcctactgcg ctttccggca gatcgtgcac cacgatgcgc gcccacggga
     7021 catccacgcg accatcgagg gtaaacaggt ttggtgtagc ctcgaataca acatctggcg
     7081 atacatccat tcgtaccatc ggcggcacgg tgatccgcac tttactgcct ttcgccgtta
     7141 ctcgtgcccg ccagttttca atttggctcc agtcggcatc accgttcagg tagatttcac
     7201 cctgctgggt ccgtactgta ccggcaagcg tcgagcgcat accgttaaag ttgaccgcaa
     7261 gctggctcgg ctgcatatca aacggcataa agttgccgtc gatatccaca cccgtaacct
     7321 gaagctgacc aaacaactgc gggctttgca catcaccacc cagacgcaag ttggcactca
     7381 ccatccccgc tgctttttcc ccacgggtaa agatggggtt tatcatcgca aggttgaagt
     7441 tacggatatt gacgttgcca ccaagattac ggcggccttg cggatcggtc acctgcacct
     7501 gtccatcaaa ctggccgtta ttggtcaggc ggatggtcca gcccaattcg gcacggttgt
     7561 tacgcaattc cgccgtcaga ttcagtgtct gaaacgccac cggcagcgcc gcatcgttga
     7621 cggtttgcgt tacctgcacg ttacgccccg aaagggtgat actgccctgc ggcagcccct
     7681 ctttcgtggt gtcccaggca acatccgctt tacccgtgaa gataccgctg gcctgagtgg
     7741 tttctggcat aaacggtttc agcatggcga ggtcgaagcg gttgagattc accaccgcac
     7801 gcccttcggc ccccgcatcg atagtttgcg gcacgcacag ttccgcattc gggttaagcc
     7861 agcagtgtgg cccgatgctg attttttgct ccttattgcg gtaatccagc gcaatatcgc
     7921 gggtcagcga ccacgggccg accggcgtct ggaagcgggt attgctaaga gttcccttcc
     7981 agcgttcttc tttgcgatca aaacttcctg ccagattaag ctgcccggag acaggctcgc
     8041 cctgaatccg caactgtagc tcgtgctgct tttcgctgcc tttggcattc agggtgacga
     8101 ggttgatatt tacatccggc tgcgaaattt gctcaacgcg tacgtcgagt ttcccggcga
     8161 tctgatcggt ggatttaatg tcgccttcca cgcgaacctg cgccacggaa agttcctgcc
     8221 agcgcaggcc gcgcgcggtg atatctgcca gtagttgtgg cgcttccacc gtgccgcgta
     8281 ctttcaccag ccctttcgct gtaccgccaa gccccggcag cgcgttatcc agccccggcg
     8341 cgttgatggt ggcatcaaga ttgagatctt ttacccccag ctcgcctttc acttcggcac
     8401 tgtttggccc gagttccaga tgaagcccag ggatcatcca ctgcatataa ctgttgcctt
     8461 tcagcgtgcc gtcaacgttc actttgttct gtttgacgtt accggtcagc ttcagttctg
     8521 gcacctccat ctgccaggtg ccgccgtaca ggctaccgcg ggttttaatc aagccattga
     8581 gtttcgacgg ccactccggg aactctttgg cggtgtaaat gccattaagc gttagctcac
     8641 cgcgccaact aatggcctgc tgccagtcga gcaacgcctt gagttcagtt ttcccttcca
     8701 gcgccgcgac ggtgagtttg tcgagattga cctgctgttc attacctttg gcatcaaggg
     8761 taatggtggc tggcgggatc tcctgtccct tcactgccgt acgcatagag agcgtgtaat
     8821 cggtcatttt gccggtaagt ttcagtttca gatcatccgc ctgatactgc ttctcaccag
     8881 tgaacggcca gtaaagctgt ttgctgttca cttccacgtt gagtggcaat ccggcttccg
     8941 ccagtcgcgt ctgggcgcgt aaatccatat ccaccggacc ggaaaggtta acgcctatct
     9001 ccagctgttc gcgcagcgcg ccgcccactt tcagcttcac cttttcacct ttcaacggct
     9061 ccacgttcag tgtgctgttg agggtgatat ccaccggcca gttgtctgac agctgcgccg
     9121 tgccgctggc gttgacgatc ccctggttgg aatcgatatc caaggcgtcc agtttagtat
     9181 tgccgtcaat gctgctcact ttcagcagca tggtgcgcac ggtgatgtcc gtgtcgcccg
     9241 tcacgcgcag ctgctcgccc ttaaactctt caatgttcag gttaagcggc aaatgcacgt
     9301 cggtcatttc cggcaatacc gggcgagaaa agagatcttt cagcgtttcg ccgagcggct
     9361 tttcctccgg ctgcggattt tcaattttcg gttcgaccac ttcttcctgc gccacttcca
     9421 ccactttcgg cagagcaatc agcaggcctt tcagcgacgt cggtttcagg gtcagggttt
     9481 tctcctgcca gttcaggccg gaggtgaagt ccatcaccga caccgtggtg tcatcaatct
     9541 tgatgttgac gttgtccagc gccacccgtg tcagggtgat gggatacggc gtggagagat
     9601 ccagcggacc gctatcttcc tcttcttcaa cctgttcaga aggaggcatt tttttactgt
     9661 cgatgttgac ctgaatgtct ttcagcgcca ggtcattaat acaaacgcta ctgttccaca
     9721 ggcactcaag accgaccgcc agatgcagat tacccgcttt taccgccacg cctagctgct
     9781 cataacgaac gtcagacaag gtgagatcgc gccagccgcc ggtgaccttg ccaatatcca
     9841 gtcctggcac ccagcgatcc gccgctttaa atacaagatg caggccgctg gtggtgccca
     9901 ccagaaacgc caccgatccc agcaacagta agataacgat aaccacgccg aggctgattt
     9961 ttttccataa actcataatt ctggccccag accgatgtaa aactgtaacc cgtgttcgtc
    10021 tttatccgcg accggtacgg caaaatcgag tttgattggc ccgaccggcg attcccagcg
    10081 tacgccgacc ccggtaccgg ttttaaagtc gctgcggcga atatcgctta ccgcttcgcc
    10141 actatcgaca aacaccgcgc cccaccattt tccggtcacg ttgtactggt attccagcga
    10201 tccggttatc aacttcgagg cccctttcag gtcaccgttg gcgtatttcg gggcgataga
    10261 tttgtatttg tagccgcgaa tactgcgatc gcccccgacg aagaaacgca gatccggcgg
    10321 tactttgtcg aaatcaccgg tttcaatcca gcccagcgta ccgcgtgtca caaagcgatg
    10381 gcgatcgtac agtgtgcgga tccagacgtt ctgcgcctgg aaaacggaga aatcgacatc
    10441 tgaaccccag gccgtgttgg agtagtcgat agagtagcgt tgcgagtctc cccaggttgg
    10501 catcaggcca ccacgagaac gcgtgcggct aatcatcacc ccaggataaa acagcatcgt
    10561 ggtgttggta atttcaccct gggtaaagtg gtcgaggctc cagcgcaggt taatggcacg
    10621 ctgccagccg ctggagagat cccagtagcg agaagccacc agcgtagtgg agtcagattc
    10681 ggtatcgttc aggtcagtgc gcttaaaacc gccctgcacc aaataatatt gttccagtgg
    10741 attcttcagc agcggcattt tatagctgaa gtcgagggtc tgttccggcg cggaaatact
    10801 ggtactggtg gtcaaactgt gaccgtatga gttcatccac ggctttttcc acgtcgcttt
    10861 cacgcgcggt cccacgtccg tagagtaacc gcccccggtt tcgatggtgt tttctgttcg
    10921 cggcgaaacc acgcccgtca atggtaatac tttcgtttcg cgcgctttat caaattgtgg
    10981 agccaccacc accgagttaa accagccggt agcagaaagt cggcggttca gttccgccaa
    11041 atctttcgat tcatactcat cgccctcttt aaacggcacc agattttgca ggtattcatc
    11101 gcggatttgt gatccttcaa aggtcacatg cccaaagcgg taacgttcgc cactgttata
    11161 atcaatatcc cagaaggctt tatgcaggcc gagcgcaatg cccagctgcg ctttggtaaa
    11221 ttcgctatcg aaataacctt tacgcaacgc aatactggtt aaggactttt tgaaattttc
    11281 ataatcgccc tggttcagta ccgtaccaat agccgggcga gtatcgagca atttcaaata
    11341 gtctttatcg gtccgcgcgc cgccgcgcaa taccacatcg gtgccgccaa ttaacaccgg
    11401 cacacctggc gtgactttgg cgatcaatac ctgccgccct ttctttggcg gtggacggag
    11461 atcaaattca atggtcggct ggtaataacc cagcgctttc agaccttcac ggatggcatc
    11521 atcgacgcgt gcgcgaaagc gacggtctgg cgtcacttca tcactttcaa tcgtagaaag
    11581 ctgcgcacga acgttctttt ccagctgtcc cgataacccc tcgacctgta gacggacgtt
    11641 cgcggcgacg gcagatccgc ttaagcagag taagcttaca cagcataact gtcggatata
    11701 gcgcacattt tctcctgaat atcctttttt cctgcccctg gaacgccgtt aaacggctta
    11761 acaaaaatcc agtaatatgg attaaaaagc agactaaacc ccaaatattt cttatgtttt
    11821 actttagacc tattcacggt ggttattgtg tgcaaatacg cctcttgtta caaccttaac
    11881 cccaatgacc gattttcggg agagcgacac catgagttta tttgataaaa agcatctggt
    11941 ttcccccgcc gatgccctgc ctggacgtaa caccccgatg cccgtagcca cgctgcatgc
    12001 ggtcaacggt cactcaatga ccaatgtacc tgacggaatg gagattgcca tttttgcgat
    12061 gggttgtttc tggggtgtgg agcgtctgtt ctggcagtta cccggccttt acagcaccgc
    12121 cgcaggctat accggcggct atacgccaaa tccgacttat cgggaagtgt gctccggtga
    12181 tacgggtcat gccgaagcgg tacgcattgt ttacgatcct tccgtcatca gctatgagca
    12241 gttgctacag gtattttggg agaatcacga tcccgcccag ggaatgcgtc agggcaatga
    12301 ccacggcacg cagtatcgtt cagcgattta tccgctgacc ccagaacagg atgccgcagc
    12361 tcgcgccagt ctggaacgtt ttcaggcggc gatgcttgcc gccgatgacg atcgtcacat
    12421 caccacggaa atcgctaacg ccacaccgtt ttattatgcc gaagatgacc accagcaata
    12481 tctgcataaa aacccatatg gttactgtgg aattggcgga attggcgtct gtctgccgcc
    12541 ggaagcatag cgttacgggt acaaatgtag tttgttgata aagtgctctt tgtctatgcc
    12601 tgatgcggct taaacgactt accaggccta caaaatcgtc caaattcaat atattgcaag
    12661 gactgcgtag gcctgatcgg catagcgcat caggcagtat tgcatttatc agcagtctga
    12721 attttaaccc tctggcgact ttacagtacc ttacgctata ctagcgactg aaaatgccgg
    12781 ttcactttct tcgaatcggc tttcaatgtg tatttcacac aaattaatca actttccctt
    12841 ccgaggatct ggcctgaaag gtcggataag atatgttaaa cagtatttta gtcatactct
    12901 gcttgatcgc tgtaagtgcg ttcttctcga tgtccgagat ctcgcttgcc gcctcacgca
    12961 aaatcaaact taaactgctg gctgatgaag gcaatataaa tgcccaacgc gttctgaata
    13021 tgcaggaaaa tcccggcatg ttctttaccg tggtccaaat cggtctgaac gcagtggcga
    13081 ttctcggcgg tatcgtcggt gatgcggcat tttctccagc ttttcacagc ctgttctccc
    13141 gctatatgtc ggcagagctc tctgagcaac tgagctttat tctctctttc tcgttagtga
    13201 ctggcatgtt tatcctgttt gcggatttaa ccccgaaacg catcggtatg attgcgccag
    13261 aagcggtggc tttgcgtatc atcaacccga tgcgcttctg cctgtacgtt tgcaccccgc
    13321 tggtgtggtt cttcaacggc ctggcgaaca tgatcttccg tattttcaaa ctgccaatgg
    13381 tacgtaaaga tgacatcact tctgatgaca tctacgcggt agtggaagcc ggtgcgctgg
    13441 cgggcgtgtt acgtaaacag gaacacgagc tgattgaaaa cgtctttgag ctggaatccc
    13501 gtaccgttcc gtcttcaatg acaccgcgtg aaaacgtgat ttggtttgat ctccacgaag
    13561 atgagcaaag cctgaagaat aaggtggcgg aacatccgca ctctaagttc ctcgtctgta
    13621 atgaagatat tgaccacatc atcggttatg tcgattctaa agacctgctg aaccgcgtgc
    13681 tggctaacca aagcctggca ctgaacagcg gcgtacaaat tcgcaacacg ctgattgtgc
    13741 cggatacgtt aaccctttca gaggcgttgg aaagttttaa aaccgcaggt gaagacttcg
    13801 cggtgatcat gaacgagtac gcgctggtgg tggggatcat caccctcaac gacgtgatga
    13861 ccacgctgat gggcgatctg gtcggtcagg ggctggaaga gcagattgtc gcccgtgatg
    13921 agaactcatg gctgattgac ggcggcaccc caattgacga cgtcatgcgc gtgctggata
    13981 ttgacgagtt cccgcagtcg ggcaactacg aaaccatcgg cggctttatg atgtttatgc
    14041 tgcgtaagat cccgaaacgc accgattcgg tgaaattcgc cggctacaaa tttgaagtgg
    14101 tggatatcga taactaccgc atcgaccagc tgctggtgac ccggatcgac agcaaggcca
    14161 ccgccctttc gccaaaactg cctgacgcta aagataaaga agaaagcgtc gcgtaacccc
    14221 cagaaacatc aacggctcct gaatcaggag ccgtcttatt actgcatagc actttggtta
    14281 agccatctct gtttgcagac gcataacctg acggttaact tcggacatca ctgacaaatg
    14341 cagcttatcc ttcacttttg ggataagaat cttaccctta tcaaattcaa aagcgccaac
    14401 gtccttgatg tataaccgtc cacggaacag gatctttacg tacttcgcca cctgaagtgg
    14461 gttgtaccgt tggaaaattt tcattgttat ctcctgctga gtattacgcc tgtgcggtgc
    14521 cacaatcggc ccaactatta tgaggcgcaa attttaatgc ttagtgacta tagactatcc
    14581 gggcaatgtt tccaccgcgt ataacttttt ttacctaaag gttacaatta ttcagaatta
    14641 tctttttacc gaagcgcggt cttcagtata agcattcatt tttcatatga tttgtgcgct
    14701 tgaccgcaaa ctggcatcac acttgcggga aattcgataa atagcacata tgattaaaac
    14761 tcagacccaa gtggtcggat cacctgcata tcataagaag gaaacaccat gaccctacgc
    14821 aagattctgg cactcacctg cctgctgttg ccgatgatgg cttccgcaca tcagttcgaa
    14881 atcggtcagc gagtgccgcc gattggcatt accgatcggg gcgagttggt gcttgataaa
    14941 gatcagttta gctacaaaac ctggaacagc gcgcagttag tgggaaaagt gcgagtactg
    15001 caacatattg ctggtcgcac ctctgcaaaa gagaaaaacg cgacgctgat tgaagcgatt
    15061 aaatcagcga agttaccgca cgatcgttac cagaccacca ccattgttaa caccgacgac
    15121 gcgattccgg gttccggcat gtttgtgcgc agcagtctgg agagtaataa aaagctttat
    15181 cccaggtcgc agtttattgt cgatagcaat ggcgtcgcgc gcggtgcctg gcagctggat
    15241 gacgaaagtt ccgctgtagt ggtgctggat aaagacggtc gcgtgcaatg ggccaaagac
    15301 ggggcgctca caccggaaga ggtgcagcaa gtgatggacc tgctgcaaaa gttgctcaaa
    15361 taatggcaat aacataaatt ctggaaaaat aattttcccc tatcgttatc tatgggatag
    15421 gggaattaag tctctgagtt cttacaccga actaatactg gtaacgctag ttgctctttt
    15481 ttaaacgcct caatgatcga tttatatttc tcgcaaatct tttctttggt aatattacta
    15541 tctgcggcca gattcgcact tactatcgca tccacttgtt cggctaattc gacttgttgg
    15601 caacctgcat taaccagaaa attataaata agagagtaat cttcatcctc caaaacaaca
    15661 gaggaaccat tacgaaaacg ttcatcccct ccatagattt taagcgcaaa gcccaaagat
    15721 tctatgacta tggcaaaatt atcatcaatt attttttcgt aaatatacca tccatcacat
    15781 tctttaattc caccgcctgg tatttggagt tcaaatttac cccgttttaa tttgcttact
    15841 acatcctcca ataaggcata accgaaagat acgtcaccaa acgcctggcg aatataacct
    15901 ttaatccttt ttggtatgtc agactgtttt ttctgcaaaa tatcacaggc at
//
LOCUS       DADLTF010000090        15914 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.090, whole genome shotgun sequence.
ACCESSION   DADLTF010000090 DADLTF010000000
VERSION     DADLTF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.090"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..396)
                     /locus_tag="JN749_002675"
     CDS             complement(<1..396)
                     /locus_tag="JN749_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HAY7369041.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTED"
     gene            complement(393..3077)
                     /locus_tag="JN749_002676"
     CDS             complement(393..3077)
                     /locus_tag="JN749_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7369042.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNTDVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            complement(3169..3669)
                     /locus_tag="JN749_002677"
     CDS             complement(3169..3669)
                     /locus_tag="JN749_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7369043.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            complement(3699..4415)
                     /locus_tag="JN749_002678"
     CDS             complement(3699..4415)
                     /locus_tag="JN749_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HAY7369044.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            4711..5487
                     /gene="exuR"
                     /locus_tag="JN749_002679"
     CDS             4711..5487
                     /gene="exuR"
                     /locus_tag="JN749_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HAY7369045.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            5832..6494
                     /locus_tag="JN749_002680"
     CDS             5832..6494
                     /locus_tag="JN749_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7369046.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            6498..6881
                     /gene="mzrA"
                     /locus_tag="JN749_002681"
     CDS             6498..6881
                     /gene="mzrA"
                     /locus_tag="JN749_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HAY7369047.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            7028..7382
                     /locus_tag="JN749_002682"
                     /pseudo
     CDS             7028..7382
                     /locus_tag="JN749_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            7420..7725
                     /locus_tag="JN749_002683"
     CDS             7420..7725
                     /locus_tag="JN749_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HAY7369048.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            7728..8132
                     /locus_tag="JN749_002684"
     CDS             7728..8132
                     /locus_tag="JN749_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369049.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            8122..8421
                     /locus_tag="JN749_002685"
     CDS             8122..8421
                     /locus_tag="JN749_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369050.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            8517..8999
                     /locus_tag="JN749_002686"
     CDS             8517..8999
                     /locus_tag="JN749_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HAY7369051.1"
                     /translation="MILSIDSNDANTAPLHKKTISSLSGAVESMMKKLEDVGVLVAHI
                     LMPILFITAGWGKITAYSGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTT
                     LFTAGFTLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIDRLLNKKW
                     "
     gene            9069..10055
                     /locus_tag="JN749_002687"
     CDS             9069..10055
                     /locus_tag="JN749_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HAY7369052.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            10348..10716
                     /locus_tag="JN749_002688"
     CDS             10348..10716
                     /locus_tag="JN749_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAY7369053.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(10923..11819)
                     /locus_tag="JN749_002689"
     CDS             complement(10923..11819)
                     /locus_tag="JN749_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7369054.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            11924..12625
                     /locus_tag="JN749_002690"
     CDS             11924..12625
                     /locus_tag="JN749_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HAY7369055.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            12648..12812
                     /locus_tag="JN749_002691"
     CDS             12648..12812
                     /locus_tag="JN749_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369056.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(13024..14316)
                     /locus_tag="JN749_002692"
     CDS             complement(13024..14316)
                     /locus_tag="JN749_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HAY7369057.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(14344..15675)
                     /locus_tag="JN749_002693"
     CDS             complement(14344..15675)
                     /locus_tag="JN749_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7369058.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTCWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
BASE COUNT         4146 a         4045 c         3682 g         4041 t
ORIGIN      
        1 atcttctgta catgaagatc tattagaatc cttaagattc gtctgaagcc ctttgccccc
       61 cttgcatcct agcggcgctt catcgctact tacaatggct gttttaaatc ctttgagttt
      121 taccggccaa cgcatcccac tgcgttgttc agttaaatag atttgtactg tcgatatatt
      181 tccattagct gtacaggttt gttggaggtc aggatacgaa cattcatacc acatatatcc
      241 attatttcct gccgacgctt catccttcca gaagtaataa tccgctctac cagatgccaa
      301 atcatacgta atttcactgg tcacgtcagg agctgcctga acgccagtga gcgtgccaaa
      361 caagcccaat atcgccgcaa tcaatcgatt tctcatggtg caacctccct ttgcgccgtc
      421 tgaacgtttc cacgtaattt aggctgtttc aactgtgcct gaacctgaat cgactccggt
      481 aattgtgaat aagtcaccac actacaagta acatctccgc tgtaacgaat gctggcgcgt
      541 ttctttacaa cactcagtgg acacttaagg aattgctgcc cggaaagcag atagagctct
      601 ttttcgctgc cgttaatttc aagtgtaaat ccgccatctt cagctaccag taacatggcg
      661 ctgttcagcc ccacaatcgg gaattttttc accgctgccg gcagtaataa acgaccgata
      721 tagttgtagt taaagctggc agacacttcg cgatagcgca gcttgccagg caacaacatt
      781 aattcacgat tccctgcacc gttaatgatg tttgttgtcc cctgctgatt gccctggcta
      841 ctgtcgttaa cctcaacaaa accagactga aatccaggat aagggaacaa cacactgtca
      901 ctttgaccaa gctgcaaagt tcggctgcct gccacccgcg cttcgagttg cagatcctga
      961 ctctcttcat cctgttcaga gaagcctttc acatccacca gtaccgcacc cagcaaatcg
     1021 tctgtaccac tggctcccaa attaacgcca tagcgactga cagccagcgt agagctataa
     1081 gtgcccgtaa aggccgaaag atgatcatga ttcttacggt cataactatc agatacggta
     1141 gcgtagacat ttccgtattg cccatcccaa cgcccgttaa ccgcgctgtt tatggtatcg
     1201 gtgttcaggc ctccgacggt tacgccaagt tctttatggc tgaatgaatc gttataaaag
     1261 gtatgcgata actgccatga cgtttgatcg ccatcctgtt cgctataacg ataatccgta
     1321 gacacattgg ttgaatggct gttgttattg ctatccatcg ttggcgtgtc agataacgaa
     1381 aacgataagt acaagccgtt gtcgtcgtta tcgccaccgt tgtcattacg atggaaaaca
     1441 ccgactgacg tactcaaaat ccagtcacgc atcacaatag cgcgggaagc gctaagttgt
     1501 acagtttcag agatactttg cgctgttgtc tggcgaagat agttattttc aaagctctgc
     1561 tgtttatccc aacgcaaact atcatcagtg ttattttgtg tgcaggtata acccagcatc
     1621 atattccagc cgacaaaatt cagtgaaact gtcgcggaaa tactttcata acagcttaag
     1681 gcgttatagc tctctctgct gtcggttgta caagcatcgc cgtcagagtt ggtccgataa
     1741 aagcccaacg tcggccatcc cggattactc cagttggcct gttgcatatc acctttgccg
     1801 ccgtcatcgt tacggaacac gctggcgctg attgcaagat tccccacccc gcctaaatct
     1861 gccgtccagt tattacctaa ctcgaaagca ctcacatcat cggcattcgc cagccctgcg
     1921 tagagctcat attgcggatg taatggcagg cgtactccta gctgataagc tgaactttca
     1981 tcatcagaaa cctgtgatgt agttttacct gcctgtaaga accattgcgc atttccgtca
     2041 gtcagaccgc cggttttggt aaacggcaca agctcggtgc gggtgagttg gttattttcg
     2101 tagactttta atgccacgct gtagctgcct ggcggaaagg aactggtatc aataaattgc
     2161 gaaccactat tgagataaaa cgatcccaaa agttgctcat tacgataagc gtcaacacga
     2221 gaattgcgcg aaagcagaac cattactggg gttccttgct ggctttgcgc ctggtttaaa
     2281 tagctcagtg tcgacccgat acgcatccca tcgattgcac cgagtggcag aaagttaaag
     2341 gtaaagttcc cgccttgtgc attaaatagt gtgcggttgt ccatgcgacc cgcctgcaca
     2401 taataacgac gtaaaaaatc ataacgataa tagagatcgc tgacgtcagt attactgtca
     2461 ctgacatcat ctgcatcata accgttgaaa ttccagtgtg caccaatata gctattttct
     2521 gttattccca gcgcaccgtt tccctgaata gacaacgatt ggtaatcatc ctgtgccaga
     2581 acattgatgt cttgctgatg tataaaagcg ttaacagtat ttttgtctgg atttaaatat
     2641 aatgacttcg catcgaaagc tgaattccat tgcggattaa taaacaacgt tgccgaactt
     2701 tcttcatcat tatagattat ttccagcgta tcggtattca gaaaaccaca ccctgagtca
     2761 gtacgtacat aaccgcaagc caattcaccg tggcgagcta gcggctgcga taatttttct
     2821 ttcactatat gcgcgatttt ctgatcgtct acatccagtt ccagcttgtt atatagactt
     2881 gctggatcaa gaaatgttac ggtatcaaga ttaaccattg cataatattt accgagactt
     2941 ttccctgaaa aataaacctc gatatactcc tgctgtccct gagcaataac ctcaaaccct
     3001 gcgggaattt tggttaatgt cgcctctgcc gggctgagac tcgccaggat caaaagagcc
     3061 agtaattttt tatccatggc aatcctgaaa tccataacaa atactaaaac agttaaaata
     3121 agggagggct acccctccct ttgttacgtg ttaattctgt tatccggatt aaatatcctg
     3181 agacagataa atgcttacta caccacggta gatgcctgtt tccagaacac ctttggtggt
     3241 ttgagcaaac atcaggtttt tagccagaga accgttagtt aacgcatcgg aagcaaagat
     3301 tttggttgcg gtaaaagtgg ttgccgcatc agctttaatt tcttcaccac cccaggttac
     3361 tgtcagcgga acaatcttgc tagcatccag gctttgaacc agttgcgctg gggacactaa
     3421 ctgcattttt acatctttgg taacgtcgtt agaccagatt ttggtcatca actggtaaga
     3481 ttgcagaccc tgccccggca gatattgcat ttctactgct ttcggcaacg cggtgttatc
     3541 ggtctgtgtc atatccagag cggcatcaac gttggccgtt acagtaatat ccttttgtac
     3601 tgccaatgcg gagcctgcaa cagaaaacat cgctgcgacc agaagagatt ttgcaaatac
     3661 ctttttcatt actattcctt cgtttattac aatgataatt aatggaccgc cagttcaaac
     3721 tcgccattat ctttggtgat ccagttatta tatttgacaa cgattttgtc gtagccagat
     3781 atgctttcaa gatttctttc ctgaccaggg aatatgtttt tatttacggt ctgtatctta
     3841 cagttttctt tgctattatt tttacaataa gcgatcgtta aaagctttaa gcgttgattc
     3901 cctgcattaa ttaatttttg ggcagaattt acttctaact tagctatagg ttgctgaggt
     3961 ggaacactaa caacgattcc ccaaattaag ttaactgtta actgcataga gagatctttt
     4021 ttatctgtgg caacgtcatc aatactggga accgcttcaa atttaacgcg ataatttttc
     4081 tctttttgct ctggttccat cgcaacaaaa cgtacggttt tgctactacc ggctggtaaa
     4141 gcgaatttag gtggcataac aaccagatga tttgcatcac cggatttaat ctcaacttca
     4201 ttttcctgag gcgtcgatgg attatcgata cggaatactg tcgccttgat gtactgaact
     4261 tcattacttt tagatattac gcgaacgttg ccttcaccct gactattaat ggaaacaggc
     4321 ataggataaa cagtcatatt tgactgtgcc aaaaaaggag cgaacacgaa taaaagcggc
     4381 gtaagccgta ataagagatt aattttcgcc accatataat tttgatctta caatatcgtt
     4441 tcaaaaaagt tggtgtatta gagatgtaga tttattgtaa gtcaatataa aaaagcattt
     4501 ttagttaaca ttaaatttaa aggctgggaa ttaacttact gaaatttagt agctttcaca
     4561 atgacgatac ttatttttta ggaatttata aatattcaca atattaattt aatacattaa
     4621 ttcaatcatc aaatttatat atgaccaatg gcatacccat gaataaagtg gtatgataac
     4681 ttcgtccgca ttccactccg ggagacgcat atggaaatca ctgaaccacg ccgtttgtat
     4741 caacaacttg ctgctgacct gaaagagcgc attgaacagg gcgtctatct ggtgggtgat
     4801 aaactgcctg ccgaacgctt tattgccgat gaaaagaacg tcagccgaac ggtagttcgt
     4861 gaagccatca tcatgctgga agttgaaggc tatgtggagg tgcgtaaagg ttcaggtatt
     4921 catgtggttt ccaaccagcc gcgtcatcag caggcggctg acaataatat ggaattcgcc
     4981 aattacggtc cgtttgagtt gcttcaggct cgccagctca tcgaaagtaa tattgccgag
     5041 ttcgcggcaa ctcaggtaac gaaacaagac atcatgaaac tgatggccat tcaggaacag
     5101 gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca cattcaggta
     5161 gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg gacccagcgt
     5221 agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac cgttgataac
     5281 tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc tcatgctgct
     5341 aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa cgaaaccagc
     5401 gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt agtgcatctc
     5461 gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc aatgcgcctg
     5521 tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc tctcgccacc
     5581 ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa cgaacaacgt
     5641 tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt tcttatctca
     5701 cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga acataccaaa
     5761 acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa ccgattcacc
     5821 aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg caggattttg
     5881 aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt gtaattttgt
     5941 tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta ctggtattgg
     6001 tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg ctgctgaccg
     6061 ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg ggcaataccc
     6121 gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc gcacaccatc
     6181 tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca tttgtcagaa
     6241 cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt cagttcttca
     6301 actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac atgctcggca
     6361 aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg ctgctcccgg
     6421 tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa aagaaatatg
     6481 gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta gcctggtccg
     6541 gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt cgccagcaag
     6601 agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac ggtttttcga
     6661 tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc aaaaacgaca
     6721 ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg gtcctcgaca
     6781 gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag gctatgcagt
     6841 ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt atccgaaaca
     6901 tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg gtggttaaca
     6961 ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca caatggaagg
     7021 ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta gtgccggtag
     7081 ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga tcagctatgc
     7141 cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga gtgaagtccg
     7201 ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg caaagcagaa
     7261 agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag gcgatgccga
     7321 tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg cgcgactact
     7381 aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga acacactacg
     7441 gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt gcttagctca
     7501 tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga gcaggcactg
     7561 aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca aacccgtgtc
     7621 gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt gggaattggc
     7681 gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg gcggacactc
     7741 atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca
     7801 tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag
     7861 ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct gcatttggtc
     7921 ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc ctgaatgcga
     7981 tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc tggacgttac
     8041 gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca aacgatcggc
     8101 aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg taaggcgcaa
     8161 ctgcttagcc agatccagca acaacggctg gatctttccg ccagtcgtcg tgaatggctg
     8221 gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg ctcctgggcg
     8281 ctggttggca gtagcgtgat ggcgatctgg acgattcgcc atcctaatat gctggtccgc
     8341 tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac gaccctcaag
     8401 cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct tatccctcaa
     8461 attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc acgtttatga
     8521 ttctctccat cgacagcaac gacgctaata ccgcgccctt gcacaaaaaa acaatcagca
     8581 gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt gtactggtag
     8641 cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc accgcttact
     8701 ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg ccgctggtga
     8761 ttctgcttga gttcggtggt ggtctggcaa tcctattcgg tttcctgact cgcaccacaa
     8821 ccctgtttac tgcgggcttt acgctgctga cggcattttt atttcacagc aactttgctg
     8881 aaggcgtcaa ctcgctgatg ttcatgaaaa acctgacaat ttctggcgga ttcctgctgc
     8941 tggcaattac cggtccgggc gcgtatagca tcgaccgcct gctgaataaa aagtggtaag
     9001 cacgcgctat acttaacgat aaaaaacgag gaggaagctc ctcgtttttg ctattggagg
     9061 agagaaaaat gggtcaactg attgacggcg tctggcatga cacctggtac gataccaaat
     9121 ctaccggcgg taaatttcaa cgttcagctt ccgcatttcg taactggctc actgccgatg
     9181 gcgcacctgg ccccactggc acaggcggtt ttatcgcaga gaaagatcgt tatcatctct
     9241 atgtttcact cgcctgcccg tgggcgcacc gcacgctgat catgcgcaaa ctcaaaggac
     9301 tggaaccgtt tatttccgtt tccgtagtga acccgctgat gctggaaaac ggctggacct
     9361 ttgatgacag ttttccggga gcaaccggag atacgctcta tcaacatgaa tttctgtatc
     9421 agctttatct ccacgccgat ccacactaca gcggacgagt tactgttccc gtgctgtggg
     9481 acaaaaagaa ccacaccatc gtcagcaacg aatcagcaga aatcatccgc atgttcaata
     9541 ccgcgtttga tgcgctgggc gcaaaagcgg gtgattacta cccaccagcc ctgcaaacga
     9601 aaattgacga acttaacggc tggatttatg acaccgttaa caacggcgtg tataaagctg
     9661 gttttgccac cagccagcaa gcctacgacg aggcggtggc gaaagtgttt gaatcgctgg
     9721 cgcgactgga gcagatttta ggccagcatc gttacctgac cggcaaccag ctaaccgaag
     9781 ccgatattcg cctgtggact acgctggtgc gttttgatcc agtgtatgtg acccacttca
     9841 agtgtgataa gcaccgcatc agcgattacc tgaatctgta tggcttcctg cgcgatatct
     9901 accagatgcc ggggatcgcc gaaactgtca atttcgatca catccgtaat cattacttcc
     9961 gcagccataa gaccatcaac cctacgggga ttatttcaat tggtccgtgg caggatctcg
    10021 atgaaccgca tggacgagat gttcgcttcg gttaaaaaaa ggcaccttac gaggtgcctc
    10081 tgataattag ctaagtgcat gttgttcatg tcggatgcgg cgtgaacgcc ttatcaaacc
    10141 tacaaagcgt ttatattcaa tatgttacaa tgaccgtgta ggccagataa gcgtagcgca
    10201 tctggcgatt ttgcgcttgc catcagtctt agcgcgcctt cacggcactt tattaattcc
    10261 ttgaacgaat atttactgcc atttcattca cgtctattct taatttgctg ctttaaaaac
    10321 aagtgattga gcaaaattga ggcaaaaatg gactggtatc tgaaagtact aaaaaattat
    10381 gtcggtttcc ggggtcgtgc gcggcgcaaa gagtactgga tgtttattct ggtcaacatc
    10441 atctttacgt tcgtgctggg gctgctggat aaaatgttag gctggcaacg cgctggtggc
    10501 gaaggcatcc tgacgacaat ctacggtatt ctggtgtttt taccgtggtg ggcggttcag
    10561 ttccgccgcc tgcacgacac cgaccgctcg gcgtggtggg cactgctatt cttaatcccg
    10621 tttatcggct ggcttattat catcgtcttt aactgccagg cgggtacgcc aggcgaaaac
    10681 cgctttggac cagatccaaa actggaacaa gaataatgat taagcacctt gtaaaaggtg
    10741 cttcaaattg ttgacaaagt gcgctttgtt tatgccggat gcggcgtaaa cgccttatcc
    10801 agcctacaaa ttgccaaaat tcaatatatt gcagtgactg cgtaggcctg ataagcgtag
    10861 cgcatcaggc agttttgcgt ttgtcagcag tcagaagcac cttgtgaaag gtgcttaata
    10921 gattattttc cagcgaacaa ctttggaatt tcccgcagac accaggattt cgcttccccc
    10981 atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc cgggctgacg
    11041 acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat cgtcgccacg
    11101 ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt taagcgcggc
    11161 tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc cgctaccgca
    11221 attccacgat atttcacacg cgtcacttca gataacggtt ccggctcctg atgaatcggg
    11281 tgatcaggcg cggcaacgta gacgttcatt aacgtataga gcttgcgcga gttgatctcc
    11341 gacgaggaac gaaaatgcat atccggcgcg ataacgatat ccgcccgccc ctgctccagc
    11401 cgttcccacg cccccgccag cacttctgtg atgattgcca gttgggtatt ggcttttgcc
    11461 gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcttc ggtcacaatg
    11521 gtgagatgcg tttcccaacc gcgcgcgaga gcttccgcat cagtagtcag tttatctgcg
    11581 gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt gaatttggtg
    11641 cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt ttgcatgatg
    11701 tagctaagtg cggaaggcac gcgtcccagc tcatccgctg ccgccgcaaa actgccccgg
    11761 cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc tttggccatt
    11821 tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac aatgtagcgt
    11881 ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta ctacccgaac
    11941 tgccaggcag tgtggacaag cagactacgg atggttgcag gcccggtata ctttttcctt
    12001 tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgtgtgc ttaaccagga
    12061 agtgctggcc ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg atattttaaa
    12121 tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg ttcaggccag
    12181 cgccggagag gcgttgctgc tctctaccca gccgggtgtc agctatagcg aacacaatct
    12241 cagcaaagac aaaccgttaa cgcgaatgca gctttggctg gatacctgcc cgcagcgaga
    12301 gaatccgctg attcaaaagc tggcgcttaa tatggacaag cagcaattaa tcgcctcgcc
    12361 agagggtgcg atgggaagcc tgcaattacg ccaacaagtg tggctgcacc atatcgtgct
    12421 cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt tgcaatcgat
    12481 tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctaa cctgcggtga
    12541 tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc cactgcgcgc
    12601 tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg agtaaaaaat
    12661 cgaccaaaaa gcgccagcta gcgaagcccg tggtggcgaa agaacctgct cgcaccgcca
    12721 aaaattttgg ctatgaagag atgttgagcg agctggaagc tatcgtcgcg gatgctgaaa
    12781 cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc ggcgtaaacg
    12841 ccttatccgg cctacaaaac acaacaacat caacaaatta aacaccccgt aggccggata
    12901 agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca acaagttgag
    12961 caccccgtag gccggataag gtgcgccagc atcgcatccg gcacaatact caaaacctgg
    13021 cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt gctgcatcga
    13081 atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg ccacgatccc
    13141 ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc acgcagccga
    13201 agcactggtc gaaaccttcg caccatcgca aatcatgccg ctgacatcgc cgatcatact
    13261 gctgatcgcc atcgagatag tttcataacg cccatccacc agccatgcca tcccggcggc
    13321 ggcccccatt gctgcggtcg ttgcggcaca aagtgcagac aaacgcggta actggttatg
    13381 gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagtcgtt catcatccgc
    13441 tccgaagtgt tctgctacaa ccaccacagg cattgttgcg gtgatcccct ggttacccga
    13501 gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg atgccgcgct
    13561 ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct cgcactgttt
    13621 ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct gcgataacgc
    13681 acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaatggga cttcattgac
    13741 gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggcg actcttgctc
    13801 gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct caatatgcac
    13861 aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt tccagacttt
    13921 ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacagaga ctttccccgc
    13981 cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca gcacttccag
    14041 cccggcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca gccccaccat
    14101 tcccgtgccg ggaacggtga tgcccagacc gttcttcatc agatttggcg aaacccaggc
    14161 ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg ccagcgccag
    14221 tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg cgaggatgta
    14281 acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa aatttcaggt
    14341 aaatcaagaa aatgccagga acagagaaac acaaagcaac aaaccagtga cgataatcag
    14401 gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca gccacgccgg
    14461 gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc tcaacaccgg
    14521 tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga tgccgcgctg
    14581 aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt tcattacgat
    14641 cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca tgacagcaaa
    14701 gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc taataaactg
    14761 tgcggcaatc gccagcgcgg aaatattctg ctcgtaggct tttaccgctt cgtcatgtcc
    14821 catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa tgccaaacgc
    14881 gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt cccgcgagcg
    14941 ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca gggtaaacgg
    15001 cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac cgacgttata
    15061 cagatgccac attcccacca tcgacacgcc cagcgcagcg actaccagca gcttggtcag
    15121 caccatgtcg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga tcgccaccag
    15181 aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt ccgtcacgcc
    15241 gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga cgaacatcca
    15301 aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt tacctaaata
    15361 accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg tattaataaa
    15421 caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca aaaataccca
    15481 caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa tcgccatccc
    15541 gatactcata atcacccagc aggtgtcagt actgtcgaat ttgatcgctt ctcgccactc
    15601 gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga cgcctttatt
    15661 cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt tttttccgag
    15721 ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa taaagaaatg
    15781 atacgcgcga ggtcagagaa aatcttcaca atcaagactt taaaataaca gagatggata
    15841 agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg gcagatcaca
    15901 aaaaaaaggt gcac
//
LOCUS       DADLTF010000091        15827 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.091, whole genome shotgun sequence.
ACCESSION   DADLTF010000091 DADLTF010000000
VERSION     DADLTF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.091"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..880)
                     /locus_tag="JN749_002694"
     CDS             complement(<1..880)
                     /locus_tag="JN749_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAY7369059.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAG"
     gene            987..1283
                     /locus_tag="JN749_002695"
     CDS             987..1283
                     /locus_tag="JN749_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7369060.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1417..1842)
                     /gene="arsC"
                     /locus_tag="JN749_002696"
     CDS             complement(1417..1842)
                     /gene="arsC"
                     /locus_tag="JN749_002696"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HAY7369061.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1855..3144)
                     /gene="arsB"
                     /locus_tag="JN749_002697"
     CDS             complement(1855..3144)
                     /gene="arsB"
                     /locus_tag="JN749_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HAY7369062.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3198..3551)
                     /gene="arsR"
                     /locus_tag="JN749_002698"
     CDS             complement(3198..3551)
                     /gene="arsR"
                     /locus_tag="JN749_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HAY7369063.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4296..4379
                     /locus_tag="JN749_002699"
     CDS             4296..4379
                     /locus_tag="JN749_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HAY7369064.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4433..5785)
                     /gene="gorA"
                     /locus_tag="JN749_002700"
     CDS             complement(4433..5785)
                     /gene="gorA"
                     /locus_tag="JN749_002700"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAY7369065.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5857..6699)
                     /locus_tag="JN749_002701"
     CDS             complement(5857..6699)
                     /locus_tag="JN749_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HAY7369066.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6902..8944
                     /gene="prlC"
                     /locus_tag="JN749_002702"
     CDS             6902..8944
                     /gene="prlC"
                     /locus_tag="JN749_002702"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HAY7369067.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8952..9704
                     /gene="rsmJ"
                     /locus_tag="JN749_002703"
     CDS             8952..9704
                     /gene="rsmJ"
                     /locus_tag="JN749_002703"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HAY7369068.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9753..11222)
                     /gene="dtpB"
                     /locus_tag="JN749_002704"
                     /pseudo
     CDS             complement(9753..11222)
                     /gene="dtpB"
                     /locus_tag="JN749_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(11528..11962)
                     /gene="uspA"
                     /locus_tag="JN749_002705"
     CDS             complement(11528..11962)
                     /gene="uspA"
                     /locus_tag="JN749_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HAY7369069.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12353..12688
                     /gene="uspB"
                     /locus_tag="JN749_002706"
     CDS             12353..12688
                     /gene="uspB"
                     /locus_tag="JN749_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HAY7369070.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12759..14258)
                     /gene="pitA"
                     /locus_tag="JN749_002707"
     CDS             complement(12759..14258)
                     /gene="pitA"
                     /locus_tag="JN749_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HAY7369071.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14490..15689
                     /locus_tag="JN749_002708"
     CDS             14490..15689
                     /locus_tag="JN749_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAY7369072.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPVLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITT
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3981 a         4048 c         4154 g         3644 t
ORIGIN      
        1 cgccggcact gccgatgatt aatgccatta atgctcccag acccattcct tttgtcatca
       61 gaacagatgc cagagggata accgcctcag cccggatata cagcggaata ccgacaacgg
      121 cgcttaatgg gatggcgaag ggattatctg ctcctgcatg agcggcaatc cacgctgagg
      181 gaataaagcc ataaataaaa gatcctatta atactcctaa caaaagatat ggcagtacat
      241 ctttaaactg ttgcaacgca tctttggtgg ctagttttat gggattaata cttactacag
      301 ctttggtact gcaacaggta actacggggt tctcaatggc tttagccgct ggactacaac
      361 aactcacttt tatgggcgta tatgtcgttc ccggcgaagt ttttgctgga gcacaacaat
      421 ttgctgatga gcttttactg gcaatgatat gacgttcaaa ccccagagaa tccaggataa
      481 tactggcaag aacggagacg ccggctgcga taatcgcgta caacaaggtg actttccaac
      541 caaaggtcat ccacattagt ccgacgataa tgggattaag taatggtgaa acaaataaaa
      601 aagtgagagt cggaccaaac ccggctttcg ctgacaacaa tccacgtaac atgggtattg
      661 tcgagcaact acagaacggg gtaacggctc ccaacagagc agccaggaga tagcctctcc
      721 cttttctggc ccccatcatc tgctggattt tatggtctgg caccttttgt cttatcaggc
      781 tgacaccggc actaatcact ataaacagca acgaaagctc gacggcgaga aacacaaaca
      841 tctctgcggc atcttgcagc atcgcaagcc aactactcat acgtaacctc attgggtaaa
      901 tctatatttc cagaataatc gaaatgaaat tcagatcaag atatttctgg tattatcgaa
      961 atatcaacca gacagagggg tgtcatatgc agttggaaga agtcgctaaa gcgctgaaag
     1021 agctaggtca cccgacccgt ttgttcatat ttaaacatct tgtcaaagcc ggggaacaag
     1081 ggttgccggt cggtgagttg caaaagcagc ttgggatacc tggctccaca ctcagccacc
     1141 atatatcagc actggtatcg gtaggactgg tcaaacaaaa ccgtgagagt cggacgctga
     1201 tgtgtgtttc gcaatacgca atgctggaag cgataattga attcttacgc gaggagtgct
     1261 gcgtaaatag caaaattgcc tgatgcgcta cgctctacat atttcatgcc atatattgaa
     1321 tttgcacggt tttgtaggtc ggataagacg cgttagcgtc gcatccgaca taaacaaagc
     1381 gcactttacc aactatcggc agtggatatc gccgctttat ttcaggcgct tacccgcttc
     1441 atcaaccact ttctcgccat cttccttgga gaatgcgcct ttttgcgcat ctggcagaat
     1501 ttccagcacc acttctgaag ggcggcacag gcgagttccc agcggcgtca ccacaatcgg
     1561 gcgattaatc agaatcgggt gctgaagcat aaagtcgatt aaccgatcgt cagtaaattt
     1621 atcttccgca aggcccagct cctcatacgg ttcgacgttt ttacgcagca gcgcgcgtac
     1681 ggaaatcccc atatcggcaa tgagtttgac cagttcatcg cgcgttggcg gagtttccag
     1741 ataatggata atggtcggtt ctgtgccgct gttgcggatc atctccagcg tattacgcga
     1801 cgtgccgcag gccgggttgt gataaatggt aatgttgctc atatcagtat ctcattacaa
     1861 agtgaaagag agacgtagcg ccagcgcagc cagcgtcaca aacagcacag gcagggtcat
     1921 gataatccct gtacggaagt aatatcccca gctgatagtc atattcttct gcgaaagtac
     1981 gtgcagccag agcagcgttg ccaggctacc aatcggggta attttcggcc ccaaatcgca
     2041 gccaatcacg ttggcataaa ccatcgcttc tttgataacg ccagatgccg tgctgccatc
     2101 aatggacaac gcgccaacca gtaccgtcgg catattgttc ataatagaag agaggaaggc
     2161 ggtgaggaat ccggtgccga gcgtcgcggc ccacaggccg ttatccgcca gcacgttgag
     2221 tacgccagaa agatattccg ttaatccggc attgcgcagg ccataaacca ccagatacat
     2281 gccgagcgag aagatgacaa tctgccaggg ggcaccgcgc aggactttac ccgtattaat
     2341 cgcatgaccg cgtttagcga cgacaaataa tatcagtgcg cccacagctg caatggcgct
     2401 caccggaatg ccgagcggtt ccaggacgaa aaatcccacc agcagaagca gtaaaacaac
     2461 ccagccagtt ttgaacgtag caggatcttt gatcgcttct gcgggagatt tcagcagcgc
     2521 catatcgtag ttctgcggaa tatctttgcg aaaatagaga tgtagcatca ccagcgtggc
     2581 aacaatcgcg gcgatatcca ccggcaccat caccgaggcg tattcgcgaa agccgaggcc
     2641 aaagaaatca gcggaaacga tattcaccaa gttggagaca ataagcggca ggctggcggt
     2701 atcggcaatg aatccggccg ccatcacgaa cgccagcgtc gtgcctttac tgaatcctaa
     2761 agccagcagc atggcgatga caatcggcgt caaaataagc gccgcgccat cattggcaaa
     2821 cagggccgca acggcggcac cgagcaggac aatccaggta aacagcaagc gaccgcgtcc
     2881 attgccccag cgtgaaacgt gcagcgctgc ccattcaaaa aagccggact catccagcag
     2941 caggctgatg ataatgacgg caataaacgc cgccgtcgcg ttccagacga tattccacac
     3001 caccggaata tcacccggat gaaccacgcc cgtaaccaac gccagtaccg cgccgagcgt
     3061 tgcactccag ccgatgccta aacctttcgg ctgccagata accaatacga tggtcaggac
     3121 aaagatagcg cctgccagta acataatgcc tcccggataa aacacatgtg caaatccata
     3181 tgtgtttagc taaattttta actgcaaatg ttcttactgt ctccggaaca gttttgtctg
     3241 gccaggttgc ggacaattgc ctgaatcttt tcctgttcac atcgccaggc ttcatcaata
     3301 attttcgccg cccatgctgg aatatgcggc gataagcggt aatgaaccca cttaccttgc
     3361 ttgcggtcca gcaatagccc gctttcacgc agcaatgcca gatggcggga tatcttgggc
     3421 tgcgactgat cgagggcagt gcagagatcg cagacgcata actctcccag ttcgctaagc
     3481 agtaaaacga tgcccagacg ggtttcatca gcaagaattt tgaacaattg gatgggtaac
     3541 agaaatgaca tattgcgctc ctgattgttg caggtagtgt ctctcttcga agcggataag
     3601 tcaaaaacat atatgactta acgaatgtgt aagtgcagag gaaggtaata ggtgtgaatt
     3661 ttgagttggc tattcatttg aaaggcggtt tgaatcagga cgtaaaaaaa accgactttg
     3721 cgtcggtttt tttactcttc agcctgagtt ggtggctctg gctggagtga gaatgcccac
     3781 atctaacggt tcaatactat tagccagtgg ctaacattgc aagcgttagc gatttcttca
     3841 gctggagaag ggacagtttg ctatacatgg tggtgaaaga ggatttatat actttcatcg
     3901 ggtcgctgcc gtaccaagcc ttgtccgaat ccggacgtaa aaaaaccgac ttcgcgtcgg
     3961 tttttttact cttcagccca gagttggtgg ctctggctgg agtgagaatg ccatcatcta
     4021 acgcatcaat gctaatacca tactgaaact attgcaaggt tatgctggtt atttagccta
     4081 attacactgt atgattatcc agttagctct gaggcatttt cactctggca atgcgcataa
     4141 acgctttcaa agtcctggtc agaagtacgg gtggtgccgt taactgatgc tctggccgga
     4201 gtgagattgc ccacatctaa caatgaggca tgcgccgtga caggcagtgg atgagtaagc
     4261 ggatgcattc tcactccatc gcatggagaa aacgggtgat tgataaagca atcatcgttc
     4321 ttggggcgtt aattgtgctg ctggaactga tccgctttct gcttcagctt ctgaactgat
     4381 agcggaaacg taattaaggg ctaagagcac actactctta gccctttaac atttaacgca
     4441 tggtcacgaa ctcttctgcc gccgttgggt gaatggcgac ggtattgtcg aagtcttttt
     4501 tggttgcgcc catcttcagc gccaccgcga agccctgcaa catttcgtcc ataccaaagc
     4561 caatgccgtg aataccgaca atcttctctt ctggcccaac gcacaccagc ttcatgcggc
     4621 acggctggcg gtgagtggtg acggcggtat acatcgcggt gaaagaggat ttatacactt
     4681 tcacctgatc gtcgccgtac tgctcgcgcg cctgcggttc cgttaaacca acagtaccaa
     4741 tcggcggatg gctgaagacc acggtcggaa tgttgctgta atccagatgc tcatccggct
     4801 tgttattaaa caagcgttca gagagacggc gacccgctgc aactgccacc ggtgtcagtt
     4861 ccactgcacc cgtgttatcg cccaccgcgt aaataccttc aatattggtg ttttgatatt
     4921 tatcgacgac gatatagcct ttttcgttgg ttttaacgcc agcggcttcg aggttgatgt
     4981 tgtcattggc aggctcgcga ccaatcgccc aaatcaggca atccaccgtt tcactgcgac
     5041 catcttccag ctccagcgtc aggctaccat cggcattttt cactaccgct ttcgggatgg
     5101 cgttggtatg caactgtggg ccttcggcgt tcatcacttc gaccagcgtt tcagagatca
     5161 tcgggtcgaa gctgcgcagc ggcgcatgtt tacgcacaaa cagatgcgtt ttcgcgccga
     5221 ggccgttaat cacgccagcc agctcaacgg cgatgtaacc cgcgccaaca accgccacgc
     5281 gctctggcaa agcaggaagg gcgaagaagc catcggaatc aataccgtat tccacgcctg
     5341 gaatatccgg gtggctcgga cgaccgccgg tggcgatcag aatatgatcg gccgtgatgg
     5401 tttcgccgtt aacttccagc gttttggcat caacgaagcg ggcaaagcct ttgattacat
     5461 caacgttatt tttaccgagc acgttttcat aggaagtatg aatacggtcg atataggcgg
     5521 tacggctggc gatcaacgtt tcccagttga acttgttgat ggtggtatcg aaaccataat
     5581 cagggccgta catatggatc gcttcacgga tttgcgccgc gtgccacatc acttttttcg
     5641 gcacgcagcc gacgttcacg caggtgccgc ccagctcttt ggcttcaatc agtgcgcatt
     5701 tctggccgta catagccgcg cggttgatgg aggcgatacc gccgctgccg ccgccgatgg
     5761 cgatgtaatc atagtgttta gtcatgacat attgtcctta tcgttgatta ccgcgattgt
     5821 agcgcgagac gtaataggtg ccagcaatgg ctgcaattac tccggcacga tccagcttac
     5881 ggtggcgtgc ccggtacctg ccggaaccag tttgctgtgc agccacgaca gcacgttatt
     5941 catctgctgt tccagcttcc acggcgggtt aatcacaatc atgccggaag cggtcatgcc
     6001 acggcgatcg ctgtctggca gcaccgccag ttcaatttgc agaattttgc gaatgccggt
     6061 cgcttccagg tcgtggatca tgcgcttaat ttgctggcgc agcaccaccg gataccacaa
     6121 tgcgtaagtg ccagtggcga aacgtttgta accttctgct atcccgctga ccaccgcctg
     6181 atagtcggtt ttcatttcat acggcgggtc gataaggatt aaaccacggc gagaaaccgg
     6241 cggcagtttg gccttaagtt gctggaaacc gtcggctttt tcgacgcgtg cacggctgtc
     6301 tttctgaaat ccagaacgca gcaacgggta atcgctcggg tgcagttcgg tcagttgcag
     6361 gctgtcttgt tcgcgcagta gctggcgagc aatcaacggc gaaccggggt agtaacgcaa
     6421 ctggccgcta cggttgaagt gttttaccac attgatgtac gcctccagtt ctgcgggcaa
     6481 atcgtcctgc tgccagatac gggcgatacc ttcgagatat tcgccggtac gctcggcatg
     6541 ttcgctacct aactgataac gcccggcccc tgcgtgggtg tcgagataga gaaacggttt
     6601 atctttctct ttcagcgact cgatgatcag gctctgaacg gtatgtttaa ggacgtcggc
     6661 gtggttgcca gcgtgaaagc tgtggcgata actgagcatg ggtaaaggtg ttccggtaag
     6721 taaaaattgg cccgtattcg gggcgcacaa aagcctatca ggacagtata ccgaaaagag
     6781 gccgccgccg cgaaagcgta acgtttccca ttgaaattca ctacacttaa ccccatgcta
     6841 cacacattat gtaaagcgcc tgttgagcgc ttccttaacc tctttaacca ggactgcgct
     6901 aatgacgaat ccgttactga ctccctttga attgcctccg ttttctaaaa ttctcccgga
     6961 acatgtcgtt ccagccgtga ctaaggcatt gaacgactgc cgcgaaaatg tggagcgcgt
     7021 agtagcgcaa ggggcaccgt acacctggga aaatctctgc cagccgttgg cggaagtgga
     7081 cgatgttttg gggcgtatct tctccccggt cagccacctg aactcggtga aaaatagccc
     7141 ggaactgcgt gaagcgtacg aacaaaccct gccgctgctg tcggaataca gcacctgggt
     7201 agggcaacat gaagggctgt ataaagcgta ccgcgacctg cgcgatggcg atcattacgc
     7261 cacgctgaac acggcgcaga agaaagcggt tgataacgcc ctgcgtgatt ttgaactctc
     7321 tggcataggt ctgccgaaag agaaacagca gcgttacggc gaaattgcta cccgtctttc
     7381 tgaactgggc aaccagtaca gcaacaacgt cctcgatgcg acgatgggct ggaccaaact
     7441 cgttaccgac gaagcggggc tggcggggat gccagaaagc gcgctggctg cggcaaaagc
     7501 ccaggccgaa gcgaaagagc tggaaggcta cctgctgacg ctggatatcc caagctacct
     7561 gccggtaatg acctactgcg acaaccaggc tctgcgcgaa gagatgtatc gcgcttacag
     7621 cacccgcgcc tccgatcaag gcccgaacgc cggtaagtgg gataacagca aggtgatgga
     7681 agagatcctc gcgctgcgtc acgaactggc gcaactgctg ggctttgaaa actacgcctt
     7741 taaatccctt gccactaaaa tggcagaaaa cccgcagcag gtgctggatt tcttaaccga
     7801 tctggcaaaa cgcgcgcgtc cgcaaggcga aaaagagctg gagcaactgc gtgccttcgc
     7861 taaagccgaa tttggtgtcg atgagttgca gccgtgggat atcgcttact acagcgaaaa
     7921 acagaaacag cacctctaca gcatcagcga cgagcagctg cgtccgtact tcccggaaaa
     7981 caaagcggtt aacggcctgt ttgaagtggt gaaacgtatt tacggcatca ccgctaaaga
     8041 gcgtaaagat gttgatgtct ggcatccgga tgtacgtttc ttcgaactgt atgacgaaaa
     8101 taacgaactg cgcggcagct tctacctcga tctgtatgcc cgtgaaaaca agcgcggcgg
     8161 ggcgtggatg gatggctgcg taggccagat gcgtaaagct gatggttctc tgcaaaaacc
     8221 ggtcgcgtat ttgacttgta acttcaaccg cccggtaaat ggtaaaccgg cgctgttcac
     8281 tcacgacgaa gtgatcaccc tgttccacga gttcggtcac ggcctgcacc atatgctgac
     8341 ccgcatcgaa accgctggtg tttccggtat cagcggtgtg ccgtgggatg cggtcgaact
     8401 gccgagtcag tttatggaaa actggtgctg ggagccggag gcgctggcgt ttatctctgg
     8461 tcactatgaa accggcgaac cgctgccgaa agagttgctg gataaaatgc tggcggcgaa
     8521 gaactaccag gcggcgctgt ttattctgcg tcagctggag ttcggcctgt ttgatttccg
     8581 ccttcatgcc gagttccgcc cggatcaggg ggcaaaaatc ctcgaaactc tggcagaaat
     8641 caagaaactg gttgccgtgg tgccatctcc gtcctggggc cgtttcccgc acgctttcag
     8701 ccatattttc gccggtggtt atgcggcagg ttactacagc tacctgtggg ctgacgtact
     8761 ggcggcagat gctttctcgc gctttgagga agagggcatt ttcaaccgtg aaaccgggca
     8821 gtcgttcctc gacaacattc tgagccgtgg cggttcagaa gagccgatgg atctgttcaa
     8881 acgcttccgt ggtcgtgaac cgcagctgga tgcgatgctg gagcattacg gcattaaggg
     8941 ctgatcattc agtgaaaatc tgcttaattg atgaaacagg cgccggagac ggcgccttat
     9001 ctgttctggc ggcccgctgg gggctggagc acgatgaaga caacctgatg gcgctggtgt
     9061 taacgccgga acatctggaa ttgcgcaagc gtgatgagcc aaaacttggc ggcatctttg
     9121 ttgattttgt tggcggagcg atggcgcacc gacgcaaatt cggcggtggt cgcggtgagg
     9181 cggtggcgaa agcggtgggc attaaaggcg attatttgcc ggatgtggtg gacgccaccg
     9241 cagggctggg gcgcgatgcc tttgtactgg cttcagtcgg ctgccgcgtg cggatgctgg
     9301 agcgtaatcc agtggttgcc gcgctactcg acgacggcct ggcgcgtggt tatgcggatg
     9361 cggaaatcgg cggctggttg caggagcggt tgcagttaat tcacgcctcc agcctgacgg
     9421 cgctaaccga tattacccca cgcccgcagg tggtctatct cgatccgatg ttcccgcata
     9481 agcagaaaag cgcgctggtg aaaaaagaga tgcgtgtctt tcagtcgctg gtggggccgg
     9541 atcttgatgc cgatggatta ctggagcctg cacgcttgct ggcgaccaaa cgcgtggtgg
     9601 taaagcgccc ggactacgcg ccaccgctgg cgaatgtcgc cacgccaaac gcggttgtga
     9661 ctaaagggca tcgctttgat atctatgcag gtacgccagt gtaaaagtaa agcagggtag
     9721 cgataacgct accctgcttt ctgcaagaat aattaatggc tttccggcgt cgcaatcatg
     9781 cgtttcagcc acggcaccat caacagcatc actactgcaa cgcccagcgt gaccagacca
     9841 atcttaccaa acacgttggt atagacgggc aacgtctcaa gcggatcggt aatgttgtcc
     9901 ggcaccgcgg taaatgttgc cacatagccg cccagcaaga acgcggcagc ctgcgtcagg
     9961 aaccacatcc cgagaataaa gcccatcaaa tgctgcggca ccagggcagc aatcatcgcc
    10021 aggccaaggg cgctaataaa caattcaccc aggctctaga ataaatacac cagcacgata
    10081 aaccatggcg atgtcagccc ctggtcatcc gcaaaccaca ttcccgcagc agcagtcgtc
    10141 aaaaagccca gtgagcacat aaacatgccg agagtaaatt tcatcggcat cgagaggtct
    10201 ttgcctttgt tacccagatg cgtgtaaatg cctgccagta ttgggctggc gagtaccacc
    10261 cagaacgggt taagcgcctg gaagctgacc gggttgatgg aaaaaccgag aatttcatga
    10321 tgcacgttgt tgatggcaaa gaagttcagc gatgttggca tctgggcgta gagaatgtaa
    10381 aacaccaccg cttcgagcat caggacaaag gcgacaaaca ttttattgcg cccggtttta
    10441 tccagcttga atgcctgacg aaagaagatg atggtgacga cgatggagag aacaatcagc
    10501 accagattgg cgacttctac gttgtgcatc agccatgcac atacgaagat catcaccacg
    10561 ctgccaagta atacgtacaa cagtttgctg aagctcatcg gcttgaagtc gggttcagaa
    10621 ccaatgtctt tcaccattcc acgacaggcg atgtaaacca gtaatgcgat aattaacccc
    10681 gccccgcaca ggttgtaggt gactgaataa ccgaatctat cagcgatcac aggggccagc
    10741 gataacgcta tcaacgagcc gatattgatc gacatataga acagggtgaa tgcgccatca
    10801 agccgcggat ctttcggcgg atagcacttc gaaagcaagc tggctgggtt agctttaaac
    10861 aggccgttac cgacagcgat agtccccagg gcgatgaaaa tcaggtcagg cttaagtagc
    10921 gacatgccgg tcatgaagta gccaatcgcc agcacaagtg ctccaagaac aatggtgcgt
    10981 ttggtcccca acaggtggtc gccgacatag ccgccaatgg aaatgaggcc atagaccagt
    11041 gcagcaaaag caccaaaagt gacaaaagcc tgctcttgcg agaatccaag ctgtttaacg
    11101 aagaaaaccg ccagtacgcc ctgcacgccg tagtagccga atcgctccca taactcgaca
    11161 aaaaagatca tgaaaaatgg gcgaggttgc tgcagcatcc ccatgggtgt tgttgtatta
    11221 atatttatta accttccaat accatcctga aaagtatgaa cgcgtaaggg ataacgcgcg
    11281 aaaaagttta catctgtcag agattcttaa tattgatgcg gtgaatagat taatacacta
    11341 ttacaacaga aaataaccag ctgatcacac taatgatggt gttaaatttt gtattggcag
    11401 gagattttga ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta
    11461 acaaggcgcg ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga
    11521 gggatgatta ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt
    11581 tgatcagctg acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa
    11641 ccaccagatc catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc
    11701 tgccgctcag ggtttcagtg attggatagc ctgcattagt ggaaagctcg gtcagcgcat
    11761 gatgtgtctc ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg
    11821 tgtataggtc agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc
    11881 gagccataga gactgctttc tctaccagaa ctttgctttc cggggagaga tcgactgcga
    11941 tgagaatgtg tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca
    12001 gctagcgttt cttgtgccgt tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa
    12061 cccagccgta aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg
    12121 tcaatccgcc ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg
    12181 gttaaccttc tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat
    12241 gttggtcacc acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg
    12301 ggagcggtag ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag
    12361 caccgtcgca ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt
    12421 ctcatcacta cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata
    12481 tgttgatgga gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt
    12541 ttggtatatc tatgcccaac gttatcgcga tcatcacgat gatgagttta ttcgccgctg
    12601 tgagcgggtg cgtcggcagt ttattctgac tagcgcattg tgtggtctgg tggtggtcag
    12661 cctgattgca ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc
    12721 gggccagtaa actgacccgc tcgttttggt gcgtacgatt acaggaactg caaggagaac
    12781 cagtacagcc cgccggaaag tagtaccgca gccggaaggg taaacaccca agccatcaga
    12841 atactggtta cggttttacg ctgtaagccg ccgccatcta ccaccatcgt ccccgcgaca
    12901 gaagaggaga gtacgtgagt ggtggaaacc ggcatcccgg tataactcgc caggccgatt
    12961 gacactgccg ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg
    13021 attttctcac cgatagtcgt tgccacacga cgccagccaa tcatcgtacc gatacctaac
    13081 gccagcgcga ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg
    13141 gacttcagtt tcttcaacag gcgctgatcg tcagcactca caccaggcat cttcaccact
    13201 ttgtcgatag tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc
    13261 gacagcttgt cgtagctttc cacatcggtg gtcaacatac ctttcaggcg gttgagcgcg
    13321 ttaatggtat tcgacggatg gcagtggaac tccgcaggtt gcgttgcgcc agcttccgga
    13381 gccggtacta actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag
    13441 taagcttcga cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc
    13501 atgttcacca cgaagcctgc tggcgcgacg ccaatcaata ccaacataac cagaccaatg
    13561 cctttctgac catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc
    13621 gcgatacgcg tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg
    13681 gtcaggtgga tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc
    13741 agaccgccag caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt
    13801 aatactttcg ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt
    13861 aaaccaatcc cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag
    13921 taccaggtac ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg
    13981 ccatgagacg atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag
    14041 gcaacactca gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg
    14101 gcgagctgag aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca
    14161 tggaaaccat tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc
    14221 ccggtatgca aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg
    14281 acatgaacgc ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt
    14341 tttttgattt gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg
    14401 caaatacttc cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc
    14461 acgcccgttt tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg
    14521 ctggtgcggc gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc
    14581 tgatcgataa tggtaaaata ctagggcgca aaatccttat gtctggcggt gggcgctgca
    14641 actttaccaa cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta
    14701 agtctgcact cgcacgtttt acccagtggg atttcattga tctggtcaat aaacacggta
    14761 tcgcctggca cgagaaaacg ttagggcaac tcttctgcga tgactccgcg cagcagattg
    14821 tcgacatgct ggtggatgag tgcgagaagg gcaatgtaac cttcagattg cgtagcgaag
    14881 tgctgagtgt ggcgaaggat gaaacaggct tcacgcttga tctgaacggc atgactgtcg
    14941 gttgcgaaaa gctggtcatc gcgaccggtg ggctgtcaat gccggtgctg ggcgcatcgc
    15001 cgtttggtta taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc
    15061 tggtgccatt cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg
    15121 cggtgccttc cgtgattacc actgaaaacg gcaccgtttt ccgtgagaac ttactcttca
    15181 cccaccgcgg cttatctgga ccggcggtgt tgcagatttc aagctactgg caaccgggtg
    15241 aatttgtcag tatcaatctg ctaccggatg tggacctcga aaccttcctg aatgagcagc
    15301 gtaacgcaca tccgaatcaa agcctgaaaa acacactggc ggttcatcta ccgaagcggt
    15361 tggttgaacg cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg
    15421 tgtgtgacca acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca
    15481 ctgaaggcta tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactttctt
    15541 cacggacgat ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg
    15601 ttaccggctg gctggggggc tataacttcc agtgggcgtg gtcgagtgca tgggcttgtg
    15661 cgcaggattt ggcgtcaatt gtgaaataac catgacgttt tcccgtcagc cgtttgtata
    15721 tgtcgttatt aagtcatttt ggtacggttc tgcatccgac attctggtgt taactcgctg
    15781 ttttgttagc gaattaacgc tattgccgtt gtggttattt tatagtg
//
LOCUS       DADLTF010000092        15788 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.092, whole genome shotgun sequence.
ACCESSION   DADLTF010000092 DADLTF010000000
VERSION     DADLTF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.092"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JN749_002709"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="JN749_002709"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAY7369073.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JN749_002710"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="JN749_002710"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAY7369074.1"
                     /translation="MSQDPFQGREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JN749_002711"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="JN749_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HAY7369075.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JN749_002712"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="JN749_002712"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAY7369076.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="JN749_002713"
     CDS             complement(5464..5661)
                     /locus_tag="JN749_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HAY7369077.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JN749_002714"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="JN749_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HAY7369078.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JN749_002715"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="JN749_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HAY7369079.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JN749_002716"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="JN749_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAY7369080.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JN749_002717"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="JN749_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HAY7369081.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JN749_002718"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="JN749_002718"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAY7369082.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JN749_002719"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="JN749_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAY7369083.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JN749_002720"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="JN749_002720"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HAY7369084.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JN749_002721"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="JN749_002721"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAY7369085.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..>15788)
                     /gene="nnr"
                     /locus_tag="JN749_002722"
     CDS             complement(14434..>15788)
                     /gene="nnr"
                     /locus_tag="JN749_002722"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7369086.1"
                     /translation="LCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLPEEAALAR
                     EAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSHPAPIVAV
                     DIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLGLDSWLAG
                     QETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEAALRAGAG
                     LVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQEWGKKALQ
                     KVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIESDRLHCAK
                     RLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGALLGQKLS
                     PYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHDESSNSAP
                     "
BASE COUNT         3530 a         4561 c         3956 g         3741 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt ccctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccaa cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgcttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgaaca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accacgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcagcatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 tgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgtgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaataaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct acctcctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgccgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac caatacggaa
    13141 gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gtgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccacaaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg ctccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagca
//
LOCUS       DADLTF010000093        15600 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.093, whole genome shotgun sequence.
ACCESSION   DADLTF010000093 DADLTF010000000
VERSION     DADLTF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.093"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..587
                     /locus_tag="JN749_002723"
     CDS             <1..587
                     /locus_tag="JN749_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HAY7369087.1"
                     /translation="SSSWGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWK
                     ALIAGGSERGLIYTQFVAQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWL
                     QSRVYARAHHYYHSWMHYFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFE
                     ELAAGSDRFYATLPWQPLTVQPECPVTLKDVSDL"
     gene            584..2035
                     /gene="djlC"
                     /locus_tag="JN749_002724"
     CDS             584..2035
                     /gene="djlC"
                     /locus_tag="JN749_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HAY7369088.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2095..3765)
                     /locus_tag="JN749_002725"
     CDS             complement(2095..3765)
                     /locus_tag="JN749_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HAY7369089.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="JN749_002726"
     CDS             complement(3849..4784)
                     /gene="rihA"
                     /locus_tag="JN749_002726"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HAY7369090.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="JN749_002727"
     CDS             complement(4902..5627)
                     /gene="gltL"
                     /locus_tag="JN749_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HAY7369091.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="JN749_002728"
     CDS             complement(5627..6301)
                     /gene="gltK"
                     /locus_tag="JN749_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HAY7369092.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="JN749_002729"
                     /pseudo
     CDS             complement(6301..7041)
                     /gene="gltJ"
                     /locus_tag="JN749_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
     gene            complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="JN749_002730"
                     /pseudo
     CDS             complement(7211..8118)
                     /gene="gltI"
                     /locus_tag="JN749_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="JN749_002731"
     CDS             complement(8515..10053)
                     /gene="lnt"
                     /locus_tag="JN749_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HAY7369093.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(10078..10956)
                     /gene="corC"
                     /locus_tag="JN749_002732"
     CDS             complement(10078..10956)
                     /gene="corC"
                     /locus_tag="JN749_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HAY7369094.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="JN749_002733"
     CDS             complement(11046..11513)
                     /gene="ybeY"
                     /locus_tag="JN749_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAY7369095.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(11510..12589)
                     /locus_tag="JN749_002734"
     CDS             complement(11510..12589)
                     /locus_tag="JN749_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAY7369096.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AREEAEQKRKAALAAERKREEQEQK"
     gene            complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="JN749_002735"
     CDS             complement(12703..14127)
                     /gene="miaB"
                     /locus_tag="JN749_002735"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HAY7369097.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14273..15448
                     /gene="ubiF"
                     /locus_tag="JN749_002736"
     CDS             14273..15448
                     /gene="ubiF"
                     /locus_tag="JN749_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HAY7369098.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         3777 a         3992 c         4059 g         3772 t
ORIGIN      
        1 tctcatcatc ctggggaata aataatcgtg atgatttaat tcagactatt tatcagatga
       61 ctgatgatgg tcatgccaat gatttagctg gcttatatct gacatggcaa cgctcttccc
      121 ctgaagagtg gaaagcatta attgctggtg gctcggagag agggttgatt tatacccaat
      181 tcgttgccca aaccgctatg tgctgtggag aagggggaat caaagcctgg gattatgttc
      241 gaatggggtt tctgtcacgg gccggtgtgc tcaacaactg gctgacggaa gaagagagtt
      301 tatggctgca atcgcgcgtc tatgcacgag ctcaccatta ctatcacagc tggatgcact
      361 atttttctgc ctattcacta gggcgtctct actggcaatc ttctcagtgc gaagacaacg
      421 cttcgctacg cgaggcgcta accctgtaca aatacgatag cgccgggagt cggatgttcg
      481 aagagctggc agcgggaagc gaccgtttct atgccacgct tccctggcag ccattaactg
      541 tccagcctga atgcccggta acgcttaagg atgtgagcga cctatgaaaa catgttggca
      601 aatcctcgaa attgaaagca cgacgcaaat agacattatc cgccaggctt atcttgctcg
      661 cttaccgttg tgtcatcccg aaaccgatcc gcaggggttt aaagcattac gccaggccta
      721 tgaagaggcg ctgcgactgg cggtaaatcc tgtcgaggaa gcagatgatg aagaaaaaga
      781 tgccaccgct gaacatgaaa tactacgtgc attcaggaca ttactggatt cagaaagtga
      841 tcgttttcag ccctccgcct ggcagaaatt tattcagcaa ttaaatacct ggaacatgga
      901 ggatgtcgat caattacgct ggccgctgtg tgcaatcgct atagaagcgc gatatctgtc
      961 attaaattgt gcttctttgt tggcagagcg tttgaactgg cattcgttta atgacagcga
     1021 aggaatggat gaggaagaaa gggaggcttt tcttgaggcc attcaggctg gtgattgttt
     1081 cgatttcctt agccttctgg aatatcccgt cgcgttacag aaccagactg ttgaatatta
     1141 cttcgcgctg gaacgttgct gccgttacca tcctgactat gtcactgcgt ttttggcgat
     1201 ggaaggaccg tggttcattc ctgatgatgc aaagttacat cgcaaactgt tgcgctggta
     1261 cagctcggtg caaacaggta tggtggaact cattcctgta gccaaacagt ggcaagcgga
     1321 agaaccagaa agcgaagatg cccggtatta ccagtgtgca caacgtttgt actgcggcga
     1381 gggggaaagc ctgcttgctg atctcggcgc gtactgggaa agttacccat ctacacaagc
     1441 tgataatttg ttgttgcagt ggagcaagcg tcattgcccg gattatttcg cgttattagt
     1501 gatggttatc gaagcgcgga gcatggtaga tgcgcaaggt caaccgctga aatatgttcc
     1561 tggtgagagc gcccggacgc ggctgttatg ggcggaaatt ttacatagcg gaaaattatc
     1621 gccgttaggt caatcgttta ttgagtcgtt attcttcaaa cgcaaagcat gggcgtggtg
     1681 gaaatcgaga gtcggtagcg agacagaaga agattcaccg ttactggatt tgtatcgggt
     1741 agcggaacag gtagtacttg aagcgtttcc gaaacaagag atgctggccc gtcttaatac
     1801 aaggctggaa ggcggagatg ctcatccatt agaggccatt atcacccgga tgctgttgac
     1861 gaaagtaaaa ctcgagccgg aggatgaaga tgtcgatgag ccaacacctg aaaaccatga
     1921 agaaaaaaat gatgagggtg aaaaaccaca gagcattacc agcattatca aaatcagttt
     1981 aacggtgctg gttataggtt atgctctcgg caaaatcgcg atgttgttta gttgacgatg
     2041 tgttttttgc gccgatgccg gatgcagcat ttacgccgca tccggcagcc gtgattatga
     2101 ggtctcaatg gctaaataaa cgctcgcccg ccgcctgacc tcatcgacgc gggccatatc
     2161 gtttgactgc agcgccgcat caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc
     2221 acccagcgcc cgcgcccaca actcttccag tttggcttta aaggtgcgat tgatgagcat
     2281 atcgcgcgga taaattttca atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc
     2341 gctcaatgtt accgggctgt ggttaatcac tctggactta acgctgccgt cttccagaag
     2401 cacgtcaact tcaagcaacc cgttgatatc ataactaaag cgaatatcaa tcgactgata
     2461 agccccggtt ttcttcaacg gcacatcgaa ggattccacc agaatgttgt ttttaacttt
     2521 gtggttttct ccctgataga cgttaaccgt aattgaatcc tgttccgggt gcatggttga
     2581 ataagtttct acacgcgaca cgggcacagt ggtgtttcgt tcaataatcg gcgagaaaat
     2641 gccggaaacg ccctggcggt taacctcaac gcccaacgag taagggcaaa tatcagtgag
     2701 gatcacttct tcaatatctt cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag
     2761 cgcgacaatg gtgctcggat cgtaactttg atacggtaat ttgccaaaca gacgcacggc
     2821 gattcgctgc accagcggca tttgtgacgc gccgccaacc agcaccagac tgtcgatttg
     2881 actcggcttc agacgcgcat cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag
     2941 cagcggcaac cacaaatctt ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcatc
     3001 ctggtactgc cagcgaatgt gcattggcga ttgattgcta cattttgccg cttccacaca
     3061 ggcgtacaag gctgccagtt cactctcgtt aagcgtggtc cttgtgacat ccgcgcgctt
     3121 taaaacgtca tcgaccaaca tatgggtaaa atcttcgcca ccgaggaagt tgtcgccagc
     3181 ggaggcgtga acttcaatca ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc
     3241 gccaccgaga tcaaacacca gcgaacgggt attttgttgg gtatgcaggc catacgccat
     3301 cgcagctgct gtgggttcat taattaagcg taccgcattt aacccggcta agtccgctgc
     3361 taaacgggta tgcttgcgtt gtttatcgct gaaataagcc ggaacggaga tcaccacatc
     3421 tttaatcgga cgttgcagaa attcttcggc atcttctttt aatgagcgta ataccaaaga
     3481 ggacagttct ggcgcgttaa atgtgtcgct gcctaaccgc cagttggtat tactgcccat
     3541 tgcacgttta aataacgctg ccgttttatc cggatgcgaa gtacgccgtg atacagccgg
     3601 ttttccgact aaaatatgat tattttcatc catgctaatt atggatggtg ttaaatattc
     3661 accgaactta tttggaatta attgcgcggc accgtctttc cagacggcaa ttaaactatt
     3721 ggtagtgccg agatcgatac ctatggcgag ttctgcatta tccattgcat ttatatcttg
     3781 aaagaaaaag agataaatag attaagacca cccaggtaac gcgtggtgat cttaatcaat
     3841 gacgagtgtt aagcgtaaaa tttcagacga tcagccagta aatcaacaaa gccctgacga
     3901 tcaacatcga caattacagt ggcattcggt ttattgccag tcagataata ataatcaaca
     3961 accgtcatac ccagggtata tttcccctgt gtttccacgc caacccagcg ctcaacagtg
     4021 gtaaacaact cgggtttcaa cagccaggcg atggtgcatg ggtcatgcag cggtgcgccg
     4081 acaaagcccc atttttcgtc tttatgatat tcgaggaaga aatccagcag ttcggcaaca
     4141 atggttgaaa cagggttgcc aatcgcgcgg aaacgctcgg tgtcttcaac gtggatttgc
     4201 gctttatgag taacatccag accggccatc accaccggga tccctgactg gaagacaatt
     4261 tctgccgctt ccgggtcaac gtaaatatta aattcagccg caggcgtcca gttaccaagc
     4321 cccattgcgc cgcccataat cacgatacgg gcaattttgc tatgcagttc cggatggcta
     4381 ttgagcagca aggcaacgtt agtttgcggt ccggtagaaa caatcgtgac aggttccgca
     4441 ctttcgcgca gcgttttcgc catcagctcc accgccgtac agttttgcgg tgcgaacgtc
     4501 ggttccggta atgccgggcc gtcgagaccg ctttcgccgt gcacattgtc cgcgataatc
     4561 aactcacgca ttaacggttt taccgcgccg cctgccaccg gaatatcggt gcgattaagc
     4621 aaggtcagca tacgcagaac attgcgtaag gttttttctg gtgtctggtt tccggcggaa
     4681 gacgtaattg ctttgacatc aagctctggt gaggcgaggg cgagaactat tgcgatagcg
     4741 tcatcatgac ctgggtcgca atctaacaga attggcagtg ccattgttgc tccttgttgt
     4801 gtgcttcttt gcgacaaggg taacgccagg atgtaacaga tacgaggggc gaaacgataa
     4861 agcgtgagat ggcgcgcaat tgggtatgcg cgccagagtg attaatgcag gattttcgcg
     4921 aggaagtctt ttgcgcggtc cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct
     4981 tcgacaattt taccctcgtc cataaagatc acccgattcg ccactttgcg ggcaaagccc
     5041 atttcgtggg tcaccaccat catggtcatt ccttcgttcg ccagttccac catcacgtcc
     5101 agcacttcgt tgatcatctc cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca
     5161 ataggagcca tacacaatgc gcgagcgatt gccacacgct gctgctgacc gccggaaagc
     5221 tgcgccggaa atttattggc atgtgcggaa agtcccacgc gctccagcaa tttcaatgct
     5281 ttttcacgag cgggcgcttt atcgcgttta agcactttca cctgcgccag ggtcaggttt
     5341 tcgataatcg acagatgagg gaacagctcg aaatgctgga ataccatccc gacgcgggag
     5401 cgcagctttg ccagatcggt tttcttgtcg ttaaccacga taccatcgac ggtgatttca
     5461 ccttgctgca ctggttcgag gccgttgacg gttttaatca gcgttgattt gccggaacca
     5521 gacgggccgc aaaccaccac cacttcgcct tttttcactt cggttgagca gtcggtcagc
     5581 acctgaaagt gaccatacca ttttgaaaca tttttcaggg taatcattat gctgtccttc
     5641 ttttcaagta gctgaccaac aacgacgcgc taaggctaat aacgaaataa acaaatccgg
     5701 caaacaggat catctcgacc tgcgtaccat cacgctcacc aatggttgag gcggtacgga
     5761 agaaatcggc cagagataac acatacacca gcgaggtatc ctggaacagt acgatgccct
     5821 gagtgagcag cagcggcacc atcgcgcgga aagcctgcgg cagaataatc agtttcatcg
     5881 actgccagtg agtcatcccc aacgccagcg cggcgctcga ctgaccacgg gaaatacttt
     5941 gaataccggc acggataatc tctgaatagt aggccgcttc aaacatcgaa aacgccacca
     6001 tcgccgaaat taaacgaata tcattttttg gcgataatcc cagcacattt tgcagaaatc
     6061 ccggcacaat caagtaaaac cacagcaaaa ccatgactaa aggaatcgag cggaatacgt
     6121 taacgtaggc tttggcaaac cacgaaacag gcgcgaagct ggacaagcgc atcaccgcca
     6181 gcatggtacc ccacaaaata ccaatcacga ctgccgtgac ggtgattttc agggtgatca
     6241 ccagcccgtc gagcagatat ggcagggaag gaacaatgga actccagtca aactcgtaca
     6301 ttatttgccc cccatgttgc cgggcaggcg aactttacgt tcaaccagcg tcatcaccag
     6361 catgataaaa gcgttaatca acacatacgt aagcgtgatg gcggtaaacg actcccaggc
     6421 gtgagccgag tagtcgagca atttacccgc ctgcgccgcc atatccacca gaccgatggt
     6481 cgaggcgatg gcggagtttt tcaccaggtt catcatctcg gaagtcatcg gcgggacgat
     6541 aacgcgataa gcattaggca gcagtacgta tcgataagtc tgcggtagcg tcaggcccat
     6601 cgccagcgcg gcattcttct gcccacgcgg tagcgactga atagcggcgc gtacctgttc
     6661 gcaaacacgg gaggcggtaa acagccccag gcagagcatg gatgaaagga aaaactgaat
     6721 attgggatcc agctcggcct taaaccacat gccgattttc tccggcagca gctccgggat
     6781 caccagatac caggtaaaga attgcacaat caacggcacg ttgcggaaca gttcgacata
     6841 caacgtaccc agaccggaga gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga
     6901 accgacgagg aaagcgataa ttcaggcgca gatcgacaaa gcgatcgtca cctgaaaacc
     6961 actccagacc caaccaagat aggtggtatt gccgaacggg gcttgttgta aaaaaatacc
     7021 ccagttccag tctatagaca taaatctact ccagaaaaaa gagggtagca gcgttaactg
     7081 ctaccctcga agattgttac ccagcgtatt gcggttttca ggcccgatgg ggaacgacca
     7141 tcaggcgtat agtctgtccg tgctacgtaa caatcgagag ggctggaatt tccgcctctg
     7201 gttcttgtaa ttagttcagt gccttgtcat tcggttcttt gaacagtgct ttcatttcgt
     7261 ctgacagttc gaaattcatg ttcaggtttt tcggcggaat tggatttttg aaccatttat
     7321 caaaccattt ttccgcttca ccggaggtct gcacctgagc gatagtgtca tccatcagct
     7381 ttttgaactg cggatcgtct ttacgcaaca tgcaaccgta ggcttcctga gactgcggct
     7441 tgccgacgat atcccagttg tctggtttct tcgctttcgc acgttcaccg gccagcagag
     7501 cgtcatccat cataaaggca acggcacgac cgctttccag ggtgcggaaa gagtcaccgt
     7561 gatctttggc gctgatgatg cgcatattca ttttttgctc ttcattcagt ttgttgagca
     7621 aaacttcaga ggtagtgccg gaagtgacga ctacggcttt gcctttcagg tcggcaaaat
     7681 ctttgatatc gccgcccttt ttggtcaaca ggcgcgtacc gaccacgaaa atagtgtcag
     7741 agaaagccgc ctatttttgg cgttcgacgt tgttggtggt agaaccacat tcaaaatcga
     7801 aagtgccgtt ttgcagcagt ggaatacggt ttgtgaggta atcggaatca gttttacctg
     7861 caagtccggt ttgttgagtt tctttttcac tgcttcaaca atggcgttgg agtaatcctg
     7921 cgagtaaccc accacttttt gctgattgtc gtaataagag aaaggtactg aagattcacg
     7981 gtgaccgacg acaatcacac cgtttttggc gattttgtcc agcgtactgc ctgctgccgg
     8041 ggcggcgtca tctgcctgtg ccagtcctgc ggaaagcgcc agggcgagga ttgctgtggc
     8101 aggtttacgt aattgcatat ccaactcctt tatcttctgc gttaagaacg catggatacc
     8161 cgttgtgagt gtttgtgttg ttatcgtctg caactttatt gtgcagtgtt gtgcctgtta
     8221 ggtaacattt agtttggcta aatgtaaaga tattgatgtt ttattgtttg tttttgcgag
     8281 gtgcaccgca ccattctgaa gcaaaattct taaaatgcac tcttttggtg ctaccggtgg
     8341 attgctgtgg tgcaaccggg ttgtactgat gctggttcaa gctttgcctt gtataacaaa
     8401 gcaatagatg tgccaaagtt ggataggaga atattgttat ccggataatg cactgatgcc
     8461 gcatccggtg agcgtgccga aatatgggat gtattccggc acgataagaa gggattattt
     8521 acgtcgctga cgcagactca tcaacacagc agcaaaacca aacagtgccg tcagcaccca
     8581 cagcggccag ttgccggtac gtgcgtatgg tgtgagtccg gtggtcggcg tcacgttagt
     8641 ggttaacacc tcgcgggtga actgcgggat catcgcctga atctcaccct gcgggccaat
     8701 caccgccgta atgccgttgt tggtgctgcg caacagtggg cgcgccagct ccagcgcacg
     8761 cattcgcgcc atctggaagt gttgccatgg accaatagat ttaccaaacc acgcatcgtt
     8821 ggagatagtc agcaggtagt cggtatccgg gcggaagtta tcgcgcactt gctcgccgag
     8881 aatgatctcg tagcaaatag ccgcagtaag ctcaatacca tttgccgaca gcggcggctg
     8941 gatatatggc ccacggctga acgacgacat cggcagatca aagaacggtg ctaacggacg
     9001 cagaatcgac tccagcggga caaactcgcc aaacggcacc agatggtttt tgttatagcg
     9061 atcggctgat tcgtagctgt acggtgcacc tttacccagc gtgatgatgg tgttgtaggt
     9121 atcgtagcgg ttctgcttat tgagacgcgc gtcgacaatc ccggttacca gcgagctacc
     9181 tttatcacgc aactcaccgt ccagtgcttt gaggaacggt tgctgattaa tttccagatc
     9241 ggttatcgcc gactccggcc agataatcaa cgatgatttg cccatcagcg gtgccgttgc
     9301 gttgtagtaa atctttagcg tattaagaag ctggccttcg tcccatttca gcgattgcgg
     9361 aatatcgccc tgaaccatcg aaacctgaat ggttttctcc ggttgcgggg taaaccattg
     9421 gatgtaacgc agcgggaagg gtagggcaaa cagcacgacg gccaccacca gcggacgcca
     9481 gttgcgtttg accaacgcca gtgccagcag gccactaacc atcatcagca ggaagttaat
     9541 ggcttccacg cccattagcg gtgccagccc ttttaacggg ccatcaatct ggctatagcc
     9601 gaactgtaac cacgggaagc cagtcagcac ccaaccgcgc agaaactcgg tcacttgcca
     9661 gagagcaggg gcggcaatcg ccacgcgcag ccaggtggtt ttcggccaca gacgcgacag
     9721 cacgccagca aacagtccgg tatacagcga caaatacgcc gccagcagca ccactaggaa
     9781 gatgttaacc gggccaggca tcccgccaaa ggtcgcgatg ctgacataga cccagttaat
     9841 accgctgcca aagaggccaa atccccagca aaagccaata gcggcagact ggagtgggcg
     9901 acggttaaag gtcaacgcct gaagccccat cagcgaaata atcgccgcag gccagacgtc
     9961 gtaaggagag aaggccagcg ttccgcaggc accgaataat aacgccagca gcaggcgaat
    10021 gcgctggcgt tcaattaatg aggcaaaagc catgtagtta tctatccagt ttcggtttta
    10081 ttcatccagc tttggctggg gtgagtcatc cgggattttg acatgaacct gaataatacg
    10141 ccgactatcg gccatcgcca ctttgaactg gtaaccgtcg atgtcgatag tttcgccacg
    10201 cgccggaaga tgcccaaacg cctgcatcac cagaccaccg atagtgtcga cttcttcatc
    10261 gctaaagtgg gtgccgaacg cttcgttgaa gtcttcaatg gaagccagtg cgcgcacggt
    10321 ccaggtatga cgactcagct gacggaagtc gatatcatct tcttcgtcat actcgtcttc
    10381 aatctcacca acaatcagtt ccaggatgtc ttcaatggtc accagaccgg aaaccccacc
    10441 gaattcgtca ataacgatcg ccatgtggta acgctgagag cgaaactctt tcagcatccg
    10501 gtctacgcgc ttactttcag gaacgacaac cgcctgacgt aacactttgt ccatgccgaa
    10561 ggcttcagca tcgctgcgca taaacggcag caagtctttc gccatcagaa tcccttcaat
    10621 gtgatctttg tcttcgctaa tcaccgggaa acgtgagtgg gcggactcga tgatgacatc
    10681 aaggcattcg tccagcgtct ggttgcgttt cagggtaatc atctgggagc gggggatcat
    10741 gatgtcgcga acgcgttggt ctgcgatgtc catcacccct tcgagcatat cgcgcgtatc
    10801 ttcgtcgata aggtcgttct gcccggaatc acggatcagc gccagcagtt cgtcacggtt
    10861 tttcggttca ccgtggaaaa gttggctgag taacagggag aaaaatccct tcttgttgct
    10921 tatcgtgtca ctactgtgtg aattgtcgtc gctcatggcg tcgtatgggt tctcatgtta
    10981 gttaatcaaa acgccgtcgt taatcaccaa cggcggggac gtctgccagt caaatgcctg
    11041 gcaaattatt ctttctcggc aatgtacgga tcctcatagc ccagagcaag cataatctct
    11101 gtttcgaggg cttccatttc ttctgcttcg tcatcttcaa tgtgatcgta acctaacaaa
    11161 tgcagactgc cgtgcaccac catatgcgcc cagtgcgcct ccagtggttt gccttgctcc
    11221 tgagcttcct tctcaaccac ctgacggcag ataaccagat cgcccagtag cgacatctcc
    11281 atgccaggcg gcacttcaaa cgggaaggag agcacgttgg tcggcttatc cttaccgcgg
    11341 taggtcagat tcagactgtg gctttcggcg gtatcgacca cgcgaatagt cacttccgac
    11401 tcttcctgaa actgcgggat caccgcattc agccatgttt gaaactggct ctcttccggt
    11461 aacccggaat tatcttcaca tgccagttgt aaatcgagga tcacctgact catttttgtt
    11521 cctgttcttc gcgcttgcgt tctgctgcca gcgccgcttt acgtttttgt tctgcttctt
    11581 ccctggcttc ataagcgtta acgatacgcg ccaccaccgg gtgacgaacc acgtcttcgc
    11641 tgtggaagaa gttaaagctg atctcttcga catcggccag cacttcaatg gcatgacgta
    11701 agcctgattt agtattacgc ggcaggtcga tctgtgtgac gtcgccggtg ataaccgctt
    11761 ttgagttaaa accgatacgg gtcaggaaca tcttcatctg ttcgatggta gtgttctggc
    11821 tctcatcgag aatgataaac gcgtcgttca gcgtacgacc acgcatatag gccagcggcg
    11881 cgacttcaat aacgttgcgc tcaatcagtt tctcgacttt ctcaaagccc agcatttcaa
    11941 acagcgcgtc gtacagtggg cgcagatacg ggtctacttt ctggcttaaa tcgccaggca
    12001 ggaagcccag tttctcaccg gcttcgaccg ccggacgagt cagcagaata cggcgaattt
    12061 cctgacgctc cagggcatca actgccgcag ccactgccag gtaggtttta cccgtacccg
    12121 ccgggccaac gccgaaggta atgtcatggt cgagaatatt ggcgatgtac tgcgcctggt
    12181 ttggcgtgcg cggcttaatt acgccgcgtt tagttttgat attgaccgct ttgccgtact
    12241 ccggcacgct ctccgcgctt tgctccagta cccgcgcttc tttaatcgca aggtggatct
    12301 gttccggttc gatatcctga atctgaccgc gcatcggggc agtatcgaca tacaggctac
    12361 gcagaatgtc tgccgcagcg gtgacgcaaa tcggacggcc ggtcagttta aagtggttat
    12421 cgcggcgatt gatctcgatg ccgagacggc gttcgagctg cttgatgttg tcatcaaacg
    12481 ggccgcacag gctcaacaga cgcgcattgt ctgctggctc cagggtgatt tcgcgagtgt
    12541 ctatgttcaa accgttcctc ttatctgtat gccgccggaa gctgaacatt caccggccta
    12601 taaggaaatt attcacgcca caggaaaaag gcgcaagcat tgcgataaag atggggataa
    12661 agagagaaaa aacaaggccc accggaacgg caggcctgag aattacggct gataataacc
    12721 cacgccaagg tcgttttctt tgcgggtgcg ggcaatcacc gactccggtg tttctgccat
    12781 gcgcagaccc atttcatctt cagttcgcac cactttaccg cgcagagagt tcgggtagac
    12841 gtcggtaatt tctacatcga cgaatttacc gatcatatcc ggtgtgccct cgaagttgac
    12901 cacgcggtta ttttcggtac gcccggaaag ctccatgatg ctcttacgcg aagtcccttc
    12961 taccagaata cgctgggtgg tgccgagcat ccgacggctc catgccatcg cttgctgatt
    13021 gatacgttct tgcagaatat acagacgctg cttcttctct tcttccggaa catcatcaac
    13081 catatcggcg gcaggcgttc ctggacgtgc agagaagata aagctgtagc tcatgtcgaa
    13141 attgacgtcg gcaatcagct tcatcgtttt ctcgaagtct tcggtggttt cgccagggaa
    13201 gccaacaatg aagtcagaac tgatctgaat atccggacgc gccgcacgca gtttacggat
    13261 gatcgctttg tactccagcg ccgtatgggt acgtcccatc aggttcagaa tgcgatcgga
    13321 accgctctgt accggcagat gcaggaagct caccagctca ggcgtgtcgc ggtacacttc
    13381 gatgatatcg tcggtgaatt cgatcggatg gctggtggta aagcgaatac gatcgatccc
    13441 gtcgatcgca gcaaccagac gcaacagatc ggcaaacgat ccggtggtgc cgtcgtagtt
    13501 ctcaccacgc caggcattca cattctgacc gagcaggttg acttcacgca cgccctgagc
    13561 cgcaagctgg gcaatctcaa acagaatatc gtcagacgga cggcttacct cttcaccacg
    13621 ggtgtaaggc accacgcagt aggtgcaata tttattgcag ccttccatga tggagacaaa
    13681 cgcggtcggc ccttcggcgc gcggttccgg cagacggtca aacttctcga tttccgggaa
    13741 gctgatatct acaaccgggc tgcggtcgcc gcgcacggag ttgatcatct ccggcagacg
    13801 gtgcagcgtt tgcggcccaa aaataatatc gacatagtgg gcgcgctggc gaatgtgctc
    13861 gccttcttgc gatgccacgc agccaccgac gccgataatc aggtctggat tcttctcttt
    13921 taacagtttc cagcgaccca actgatggaa gactttttcc tgagccttct cgcggattga
    13981 gcaggtgttc agcagtagca catccgcttc ttccgccacg tcggtcagtt gatagccgtg
    14041 ggtggcatcc agcagatcgg ccatcttcga tgaatcgtac tcgttcatct gacagcccca
    14101 ggttttaata tggagttttt tggtcatcga cttgctcttg cgaaatagta gccaggaatg
    14161 cagggcgcat agtgtaatgc tttgctgccg ttgtgaccag tatgagcgtt atcagccctt
    14221 gggggtaaaa atcctgtaaa cttaaagcat attgctaaca ggatgattga ccatgacaaa
    14281 tcaaccaacg gaaattgcca ttgtcggcgg aggaatggtc ggcggcgcac tggcgctggg
    14341 gctggcacag cacggatttt cggtaacggt gatcgagcac gcagaacctg caccgtttgt
    14401 cgctgacagc caaccggacg tgcggatctc ggcgatcagc gcggcttcgg tatcattgct
    14461 taaaggggta ggggtctggg atgcagtaca ggctatgcgt tgccatcctt accgcagact
    14521 ggaaacctgg gagtgggaaa cggcacatgt ggtatttgac gcagccgaat taaagttgcc
    14581 gttgcttggc tacatggtgg aaaacacagt cctgcaacag gcgttgtggc aggcgctgga
    14641 agcgcatccg aaagtaacgt tacgtgtgcc aacctcgctg attgcattac atcgagataa
    14701 tgatcttcag gagctggaac tgaagggcgg tgaaacgatc cgcgcgaaac tggtgattgg
    14761 tgccgacggc gcaaattctc aggtgcggca gatggcggga attggcgttc atgcctggca
    14821 gtatgcgcag tcgtgcatgt tgattagcgt acagtgtgag aacgatcccg gcgacagcac
    14881 ctggcagcaa tttaccccgg acggaccgcg tgcgtttctg ccgttgtttg ataactgggc
    14941 atcgctggtg tggtatgact ctccggcgcg tattcgccag ttgcagaata tgaatatggc
    15001 gcaactccag gcggaaatcg cgaagcattt cccgtcgcgt ctgggttacg ttacaccgct
    15061 tgccgctggt gcgtttccgc tgacgcgccg acatgcgttg cagtatgtgc agccggggct
    15121 tgcgctggtg ggcgatgccg cgcataccat ccatccgctg gcggggcagg gggtgaatct
    15181 tggttatcgt gatgtcgatg ccctgattga tgttctggtg aatgcccgca gctacggtga
    15241 agcgtgggcc agttatcctg tcctcaagcg ttaccagatg cggcgcatgg cggataactt
    15301 cattatgcaa agtggtatgg atctgtttta tgccggattc agtaataatc tgccgccgct
    15361 gcgttttgtg cgtaatctcg ggttaatggc ggcagagcgt gctggcgtgt tgaaacgtca
    15421 ggcgctgaaa tatgcgttag ggttgtagcc ttacaacatt gccggatgcg tgccaaccgt
    15481 aggtcggata agacgcggca gcgtcgcatc cgacattgaa ggataagacg tgtcaacatc
    15541 gcattcgaca ttgaatgaac gcagaaaagc aaaaagctcg ccgaagcgag cttttttaat
//
LOCUS       DADLTF010000094        15541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.094, whole genome shotgun sequence.
ACCESSION   DADLTF010000094 DADLTF010000000
VERSION     DADLTF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.094"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            216..410
                     /gene="bfd"
                     /locus_tag="JN749_002737"
     CDS             216..410
                     /gene="bfd"
                     /locus_tag="JN749_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HAY7369099.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            483..959
                     /gene="bfr"
                     /locus_tag="JN749_002738"
     CDS             483..959
                     /gene="bfr"
                     /locus_tag="JN749_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HAY7369100.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(956..1423)
                     /locus_tag="JN749_002739"
     CDS             complement(956..1423)
                     /locus_tag="JN749_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAY7369101.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            1802..2113
                     /gene="rpsJ"
                     /locus_tag="JN749_002740"
     CDS             1802..2113
                     /gene="rpsJ"
                     /locus_tag="JN749_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAY7369102.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2146..2775
                     /gene="rplC"
                     /locus_tag="JN749_002741"
     CDS             2146..2775
                     /gene="rplC"
                     /locus_tag="JN749_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAY7369103.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2786..3391
                     /gene="rplD"
                     /locus_tag="JN749_002742"
     CDS             2786..3391
                     /gene="rplD"
                     /locus_tag="JN749_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAY7369104.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3388..3690
                     /gene="rplW"
                     /locus_tag="JN749_002743"
     CDS             3388..3690
                     /gene="rplW"
                     /locus_tag="JN749_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAY7369105.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3708..4529
                     /gene="rplB"
                     /locus_tag="JN749_002744"
     CDS             3708..4529
                     /gene="rplB"
                     /locus_tag="JN749_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAY7369106.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4546..4824
                     /gene="rpsS"
                     /locus_tag="JN749_002745"
     CDS             4546..4824
                     /gene="rpsS"
                     /locus_tag="JN749_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAY7369107.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4839..5171
                     /gene="rplV"
                     /locus_tag="JN749_002746"
     CDS             4839..5171
                     /gene="rplV"
                     /locus_tag="JN749_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAY7369108.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5189..5890
                     /gene="rpsC"
                     /locus_tag="JN749_002747"
     CDS             5189..5890
                     /gene="rpsC"
                     /locus_tag="JN749_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAY7369109.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5903..6313
                     /gene="rplP"
                     /locus_tag="JN749_002748"
     CDS             5903..6313
                     /gene="rplP"
                     /locus_tag="JN749_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAY7369110.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6313..6504
                     /gene="rpmC"
                     /locus_tag="JN749_002749"
     CDS             6313..6504
                     /gene="rpmC"
                     /locus_tag="JN749_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAY7369111.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6504..6758
                     /gene="rpsQ"
                     /locus_tag="JN749_002750"
     CDS             6504..6758
                     /gene="rpsQ"
                     /locus_tag="JN749_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAY7369112.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6923..7294
                     /gene="rplN"
                     /locus_tag="JN749_002751"
     CDS             6923..7294
                     /gene="rplN"
                     /locus_tag="JN749_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAY7369113.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7305..7619
                     /gene="rplX"
                     /locus_tag="JN749_002752"
     CDS             7305..7619
                     /gene="rplX"
                     /locus_tag="JN749_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAY7369114.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7634..8173
                     /gene="rplE"
                     /locus_tag="JN749_002753"
     CDS             7634..8173
                     /gene="rplE"
                     /locus_tag="JN749_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAY7369115.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8188..8493
                     /gene="rpsN"
                     /locus_tag="JN749_002754"
     CDS             8188..8493
                     /gene="rpsN"
                     /locus_tag="JN749_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAY7369116.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8527..8919
                     /gene="rpsH"
                     /locus_tag="JN749_002755"
     CDS             8527..8919
                     /gene="rpsH"
                     /locus_tag="JN749_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAY7369117.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8932..9465
                     /gene="rplF"
                     /locus_tag="JN749_002756"
     CDS             8932..9465
                     /gene="rplF"
                     /locus_tag="JN749_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAY7369118.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9475..9828
                     /gene="rplR"
                     /locus_tag="JN749_002757"
     CDS             9475..9828
                     /gene="rplR"
                     /locus_tag="JN749_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAY7369119.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9843..10346
                     /gene="rpsE"
                     /locus_tag="JN749_002758"
     CDS             9843..10346
                     /gene="rpsE"
                     /locus_tag="JN749_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAY7369120.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10350..10529
                     /gene="rpmD"
                     /locus_tag="JN749_002759"
     CDS             10350..10529
                     /gene="rpmD"
                     /locus_tag="JN749_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAY7369121.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10533..10967
                     /gene="rplO"
                     /locus_tag="JN749_002760"
     CDS             10533..10967
                     /gene="rplO"
                     /locus_tag="JN749_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAY7369122.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10975..12306
                     /gene="secY"
                     /locus_tag="JN749_002761"
     CDS             10975..12306
                     /gene="secY"
                     /locus_tag="JN749_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAY7369123.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12338..12454
                     /gene="rpmJ"
                     /locus_tag="JN749_002762"
     CDS             12338..12454
                     /gene="rpmJ"
                     /locus_tag="JN749_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAY7369124.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12601..12957
                     /gene="rpsM"
                     /locus_tag="JN749_002763"
     CDS             12601..12957
                     /gene="rpsM"
                     /locus_tag="JN749_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAY7369125.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12974..13363
                     /gene="rpsK"
                     /locus_tag="JN749_002764"
     CDS             12974..13363
                     /gene="rpsK"
                     /locus_tag="JN749_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAY7369126.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13397..14017
                     /gene="rpsD"
                     /locus_tag="JN749_002765"
     CDS             13397..14017
                     /gene="rpsD"
                     /locus_tag="JN749_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAY7369127.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14043..15032
                     /gene="rpoA"
                     /locus_tag="JN749_002766"
     CDS             14043..15032
                     /gene="rpoA"
                     /locus_tag="JN749_002766"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAY7369128.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15073..15456
                     /gene="rplQ"
                     /locus_tag="JN749_002767"
     CDS             15073..15456
                     /gene="rplQ"
                     /locus_tag="JN749_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAY7369129.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4003 a         3650 c         4140 g         3748 t
ORIGIN      
        1 ggcgcgggcg ttgttgctaa agttctgggc taattacacg ttaattagtt ttgaattgaa
       61 aagggcgctt cggcgccctt tttgctgccc gtaatttgag ctcgaaatat ttagtcgtaa
      121 ttttgctcgc tgaactatgg cgcaaataaa actgtgctat tgtaatcata accattctca
      181 tttacacttt gtgcagaatt tgaacgggag caatcatgta cgtttgtttg tgtaatggtg
      241 taagtgacaa aaaaattcgc caggcagtcc gtcagtttca tccgcaatct ttccaacaat
      301 tgcgtaagtt tattcctgtg ggaaatcaat gcggtaagtg cattcgcgcc gcgcgcgaag
      361 tgatgcagga cgagttgatg cagatgccag aatttaaaga gattgcctga agcccacgct
      421 tattttttga catcccgcta gccccatcta cgctctaata gtggaagcgg agggactata
      481 aaatgaaagg tgatgttaaa atcataaatt atctcaataa actattggga aatgagcttg
      541 tcgcaatcaa tcagtatttt ctccatgccc ggatgtttaa aaactggggc ctgatgcgtc
      601 tcaatgacgt cgaataccat gaatccatcg atgagatgaa acacgctgat aaatatatcg
      661 agcgtatttt gtttttagaa ggaatcccca acttacagga tttaggcaaa ctggggattg
      721 gtgaagatgt cgaggagatg ctgcaatctg acctgcgatt agaactggaa ggcgcgaagg
      781 atctgcgtga ggctatcgca tatgccgaca gcgttcatga ctatgttagc cgcgatatga
      841 tgatcgagat ccttgctgat gaagaaggtc atatcgactg gctcgaaact gaactggatc
      901 tcatcggtaa aatcggccta caaaattacc tgcaatcaca aattaaagtt aaagattaac
      961 ggcctatcag caaactatcc cagcctataa cgaaacccgc agccgccaga aatggcccaa
     1021 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
     1081 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
     1141 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
     1201 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgcc ccccataacg
     1261 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
     1321 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
     1381 ataaacaagc gtacaaaatt agaaagggaa gggtagtgtc cataaaacat cctctgtaat
     1441 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
     1501 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
     1561 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
     1621 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
     1681 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
     1741 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
     1801 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
     1861 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
     1921 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
     1981 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     2041 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     2101 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     2161 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     2221 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     2281 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     2341 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     2401 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     2461 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     2521 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     2581 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     2641 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     2701 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     2761 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     2821 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     2881 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     2941 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     3001 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     3061 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     3121 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     3181 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     3241 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     3301 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     3361 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     3421 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     3481 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     3541 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     3601 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     3661 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     3721 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     3781 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     3841 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     3901 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     3961 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     4021 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     4081 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     4141 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     4201 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     4261 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     4321 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtactgcga
     4381 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     4441 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     4501 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     4561 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     4621 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     4681 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     4741 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     4801 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatt gctaaacatc
     4861 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     4921 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtacaggtca
     4981 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     5041 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     5101 gtgcaaaagg tcgtgcagat cgtatcctga agcgcaccag ccacatcact gtggttgtgt
     5161 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     5221 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     5281 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     5341 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     5401 tcgcccgggt atcgttatcg gtaagaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     5461 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     5521 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     5581 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     5641 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     5701 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     5761 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     5821 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     5881 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     5941 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     6001 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     6061 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     6121 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     6181 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     6241 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     6301 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcattgaaga gctgaacacc
     6361 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     6421 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     6481 ctgaacgaga aggcgggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     6541 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     6601 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     6661 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     6721 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatatg
     6781 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     6841 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     6901 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     6961 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     7021 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     7081 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     7141 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     7201 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     7261 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     7321 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     7381 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     7441 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     7501 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     7561 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     7621 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     7681 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     7741 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     7801 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     7861 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     7921 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     7981 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     8041 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     8101 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     8161 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     8221 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     8281 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     8341 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     8401 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     8461 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     8521 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     8581 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     8641 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     8701 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     8761 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     8821 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     8881 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     8941 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     9001 attacgatca aaggtaaaaa cggcgagctg acccgtactc tcaacgatgc tgttgaagtt
     9061 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     9121 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     9181 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg caatgtgatt
     9241 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     9301 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     9361 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt acaaaggcaa gggtgttcgt
     9421 tacgccgacg aagtcgtgcg taccaaagag gctaagaaga agtaaggtaa cactatggat
     9481 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     9541 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     9601 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     9661 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     9721 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     9781 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     9841 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     9901 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     9961 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
    10021 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
    10081 ctctacaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
    10141 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
    10201 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
    10261 ctattgatgg cctggaaaat atgaattctc cagaaatggt cgctgccaag cgtggtaaat
    10321 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
    10381 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcttg ggtctgcgtc
    10441 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
    10501 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
    10561 aaggctccaa aaaggcgggt aaacgcctgg ggcgtggtat cggttctggc ctcggtaaaa
    10621 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
    10681 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
    10741 aagcagcgat tacagccgaa gttcgtctat ctgacctggc taaagtagaa ggcggtgttg
    10801 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
    10861 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
    10921 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
    10981 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    11041 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    11101 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    11161 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    11221 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    11281 gcagaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgttac
    11341 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    11401 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    11461 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    11521 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    11581 attgcccata ctatcgagca agcgcgtcaa ggtgacctgc acttcctcgt gttgctgttg
    11641 gttgcagtat tagtatttgc agtgacgttc tttgttgtgt ttgttgagcg tggtcaacgc
    11701 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    11761 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    11821 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    11881 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    11941 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    12001 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    12061 gcgaagtata tcgataaagt aatgacccgc ctgaccttgg ttggtgcgct gtatattacc
    12121 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    12181 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    12241 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    12301 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa
    12361 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    12421 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    12481 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    12541 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    12601 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    12661 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    12721 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt
    12781 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    12841 atggatcttg gttgctatcg cggtttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    12901 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    12961 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    13021 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    13081 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    13141 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    13201 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    13261 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    13321 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    13381 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    13441 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    13501 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    13561 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    13621 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    13681 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    13741 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaaca tcgcttctta
    13801 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    13861 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    13921 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    13981 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    14041 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    14101 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    14161 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    14221 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    14281 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    14341 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    14401 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    14461 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    14521 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    14581 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
    14641 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    14701 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    14761 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    14821 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    14881 atctggtaca gcgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    14941 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcttggaaa
    15001 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    15061 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    15121 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    15181 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    15241 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    15301 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    15361 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    15421 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    15481 ccccggcggg tttttttata cccgtagtat ccccacttat ctacaatagc tgtactcttt
    15541 t
//
LOCUS       DADLTF010000095        15442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.095, whole genome shotgun sequence.
ACCESSION   DADLTF010000095 DADLTF010000000
VERSION     DADLTF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.095"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(210..2051)
                     /gene="rpoD"
                     /locus_tag="JN749_002768"
     CDS             complement(210..2051)
                     /gene="rpoD"
                     /locus_tag="JN749_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAY7369130.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(2246..3991)
                     /gene="dnaG"
                     /locus_tag="JN749_002769"
     CDS             complement(2246..3991)
                     /gene="dnaG"
                     /locus_tag="JN749_002769"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAY7369131.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(4102..4317)
                     /gene="rpsU"
                     /locus_tag="JN749_002770"
     CDS             complement(4102..4317)
                     /gene="rpsU"
                     /locus_tag="JN749_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAY7369132.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4555..5568
                     /gene="tsaD"
                     /locus_tag="JN749_002771"
     CDS             4555..5568
                     /gene="tsaD"
                     /locus_tag="JN749_002771"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAY7369133.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(5611..7008)
                     /locus_tag="JN749_002772"
     CDS             complement(5611..7008)
                     /locus_tag="JN749_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HAY7369134.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALMLLLRIISWDDIVSNKAAWNVFFWLASLITLATGLNN
                     TGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRYFFASATAYTSALAPMMIAAALA
                     MPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSGYLPTADYWRLGAIFGLIFLV
                     LLVITGLLWMPVVLL"
     gene            complement(7056..7661)
                     /gene="ttdB"
                     /locus_tag="JN749_002773"
     CDS             complement(7056..7661)
                     /gene="ttdB"
                     /locus_tag="JN749_002773"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HAY7369135.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(7658..8501)
                     /gene="ttdA"
                     /locus_tag="JN749_002774"
                     /pseudo
     CDS             complement(7658..8501)
                     /gene="ttdA"
                     /locus_tag="JN749_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            8708..9639
                     /gene="ttdR"
                     /locus_tag="JN749_002775"
                     /gene_synonym="dan"
                     /pseudo
     CDS             8708..9639
                     /gene="ttdR"
                     /locus_tag="JN749_002775"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            complement(9652..10269)
                     /gene="plsY"
                     /locus_tag="JN749_002776"
     CDS             complement(9652..10269)
                     /gene="plsY"
                     /locus_tag="JN749_002776"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAY7369136.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            10374..10742
                     /gene="folB"
                     /locus_tag="JN749_002777"
     CDS             10374..10742
                     /gene="folB"
                     /locus_tag="JN749_002777"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HAY7369137.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10833..11654
                     /gene="bacA"
                     /locus_tag="JN749_002778"
     CDS             10833..11654
                     /gene="bacA"
                     /locus_tag="JN749_002778"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7369138.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11817..13055)
                     /gene="cca"
                     /locus_tag="JN749_002779"
     CDS             complement(11817..13055)
                     /gene="cca"
                     /locus_tag="JN749_002779"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HAY7369139.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(13119..13739)
                     /locus_tag="JN749_002780"
     CDS             complement(13119..13739)
                     /locus_tag="JN749_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HAY7369140.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            13981..15282
                     /locus_tag="JN749_002781"
     CDS             13981..15282
                     /locus_tag="JN749_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HAY7369141.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15233..>15442
                     /locus_tag="JN749_002782"
     CDS             15233..>15442
                     /locus_tag="JN749_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369142.1"
                     /translation="MRQTIRNRSGCTAENVNQARIKSLMKPLSSPLQQYWQTVVERLP
                     ELLAEESLSAQAKSVLTFSDFVQDSV"
BASE COUNT         3528 a         3977 c         4192 g         3745 t
ORIGIN      
        1 gttacgcaga gaatcgtaac gggataccag atcaagagcc tgtccaatgt gcataaaaaa
       61 atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg tcaactgatc
      121 cttaactgat cggcattaca ccgaaaaggt ggcgtttgtg cagagagact agtgccgggt
      181 gcggcgtaac gcctgatccg gcctaccgat taatcgtcca ggaagctacg caacacttca
      241 gaacggctcg ggtgacgcag tttgcgcagc gccttcgctt cgatctgacg gatacgttcg
      301 cgggtaacgt cgaactgttt acccacttct tccagcgtgt ggtcggtgtt catatcgata
      361 ccgaaacgca tacgcagaac ttttgcttca cgcgcggtca ggccagccag cacgtcgtgc
      421 gttgccgcac gcaggctttc ggtggtcgca gaatccagcg gcagctcgag ggtggtatcc
      481 tcgatgaaat cccccagatg cgaatcttca tcatcaccga tcggcgtttc catggagatt
      541 ggctctttgg cgatcttcag cactttgcgg atcttgtctt ccggcatcag catacgttca
      601 gccagttctt ccggcgtcgg ttcacggccc atctcttgca gcatctggcg agaaatacgg
      661 ttgagtttgt tgatagtctc aatcatatgc accggaatac ggatggtgcg cgcctgatcc
      721 gcgatagaac gggtgatcgc ctgacggatc caccaggttg cgtaggtgga gaacttgtaa
      781 ccacggcggt attcgaattt atcaaccgct ttcatcaggc cgatgttacc ttcctgaatc
      841 aggtcaagga actgcaagcc acggttggtg tatttcttgg cgatagaaat aaccagacgt
      901 aagttcgctt caaccatctc tttcttcgca cggcgggctt tcgcttctcc gatggacata
      961 cgacggttga tatctttaac ctgctcgatg gtcaggccgg tttcttcttc aatctgctgc
     1021 agtttttgca gggcgcgatg cacttcttca gagacatcgt gcagtttttc cgaccacggc
     1081 ttgttcatcg caattgccgc gttgaaccag gtatcgctgg tttcgttgcc ggtaaacagg
     1141 gtaatgaagt ttttcttcgg cattttgcac tgctcaacgc agagcttcat gatcagacgt
     1201 tcttgcgtac gaacgcggtc catcatgacg cgcatgctgt tgaccaggta gtcaaactgc
     1261 ttcggcacca ggcggaactg tttgaatact tcagacagtt tcaggatctc ttcctgagcg
     1321 gcagcgtgac tgcgaccttt cgctttgatg gtgtcacgcg ttacaacgta ctgagcgcgt
     1381 agttccgcaa atttttcgcg agccagttcc gggtcgatgc tgttgtcatc atcggcgctg
     1441 tcgtcatcgc catcttcttc gtcttcatct tcgtcatcgt ccagatcttc ctgggaaagc
     1501 tcagaaccga cgtgagtggc ggtaggtgcc agatcttctt ctgcgttcgg gtcaacaaag
     1561 ccggtgatca gatcggacag acgcgcttct tctgcttcaa cacgatcgta ctgttccagc
     1621 agataggtga tcgcttccgg atattcagca acggagcatt gaacttggtt gatcccgtct
     1681 tcaatacgct tagcgatgtc aatttcgcct tcgcgggtca acagttcaac ggtgcccatt
     1741 tcacgcatgt acatgcgtac cgggtcagtc gtgcgcccga tttcagattc cacgctggaa
     1801 agcacctgcg cggcggcttc ggcagcatct tcgtccgcgg tgttttcagc cagcatcaga
     1861 tcatcggcat ccggtgcttc ttccatcacc tgaatgccca tgtcgttgat catttggatg
     1921 atgtcttcga tctgatctga atcgacgata tcttccggca gatggtcatt gacctcggca
     1981 taggtcagat agccttgctc cttaccacgg gtgacaagaa gtttcagctg tgactgcggg
     2041 ttttgctcca taagacggta tccacactta tttcatgagg ttggtgttgg gcgattgacc
     2101 cgatgtcctt cagccgttaa cgctgaaatc caacggttgt ttaccgccaa cgataattac
     2161 gagggcgtac ttatatattt gccgctgcct ctcagtgcgg ctgtcggggg cttcccgatc
     2221 gctcttcggc acttaagccg ttaaatcact ttttcgccag ctcctggttt aatgtccaga
     2281 gctccaggcg ttcttcgttg cttaaaccat gcgtgcgctc acgagcgatt aactcttcct
     2341 ggcgcagttc aagcagcgaa tcaaacatat ggttgagtga gtcggtgaag gtttgctcag
     2401 caatattctt atctgctata tcgtcccaca tcgacagttt ttcaagggtg gcagcattat
     2461 ttgtaccacg atagtgctct aaaagttgcc cggaggtcag acctggctgg gagagacaag
     2521 tgttgaccag ttctctgaat aagccaagtc cagggagctt attttcatcc agattctcaa
     2581 gcggcgggac caacgtcgct aattctggat tttgcaccag caaccctata agtatacgca
     2641 tggtcgtgcg ttttagctgc ggaacagggc gagaaacgcc gctctctgcc gcttttggca
     2701 ttaatcgttc aagctggctg tcatcaagta tgcctaattt gttgcctaat tcctgacgaa
     2761 gatatattcg cagcgtttcg cccggcactt gcgatatcaa tggcagtgcc agcgtactca
     2821 aacgtgcgcg cccgtcaggg gtactcagat caacttgcgg catcagactg ttaaacagaa
     2881 atgcggagag tggcatcgcc tgctccatcc gcgcttcaaa cgcttcttta ccttcttttc
     2941 gtaccagcgt gtcagggtct tcgccatccg gcaaaaacat aaagcgtagc tgacggccgt
     3001 ctgtcatgta aggcagcgcc gtttccagcg ctcgccatgc ggcatcgcgg cctgcacggt
     3061 cgccgtcata acagcaaatg acattgttgg tcgcgcggaa caacagttgt atgtgatcgg
     3121 cggtggttga cgtacctaac gacgcaacgg cgtaattaat gccgtattgc gccagcgcca
     3181 ccacgtccat atagccttcg accacaagca gacgattggg ttcagcgtta tcctgctgcg
     3241 cttcataaag accgtaaagc tggcggcctt tatggaaaat gtctgtttcc ggcgagttca
     3301 ggtatttggg ggtatcgttg cccagcacgc gcccgccaaa accaatcacc cgaccgcgtt
     3361 tatcgcgaat ggggaatatc acccgttcgc ggaaacgatc gtaactgcgt ccctgatcgt
     3421 tagtgaccaa catccccgca tcaatcaatg actggcgatt ttctggattg ccgccaaacc
     3481 gcttcaggac gttgtcccag ccggggggcg caaaaccaat cgcaaagcga gcgataacct
     3541 cgtggcttaa tccgcgtttt tccagatact ggcgcgcaga cgtggcaaca ggttgttgta
     3601 aagattgttg gtaaaacgta ttcagaccgt ccatcaactg ataaagcgtt tgcctctgat
     3661 ggcgctcgat ctggctgggg ccgctgcctg cttcaaatgg cacttcaaga ttgtgcattg
     3721 ctgccagctc ttcgaccgtt tcgacgaact cgagcttgtc gtagttcatc aggaagtcga
     3781 tcgcgttgcc gtgcgcgcca catccaaagc agtggtaaaa ctgtttctca ccgttaacgg
     3841 tgaatgacgg ggttttctcg ttgtggaatg gacaacacgc gtggaaattc ttgccctgct
     3901 ttttcagctt cacacgggca tcgatcagat cgacgatgtc agtgcgtgcc agcagatcat
     3961 taatgaatac gcgtgggatt cgtccagcca taggccccga tttttagcaa ttcataaacg
     4021 aaaataagcc gcgcattcct ttcggaagca cggccttaca actacaactc ggtctgatcg
     4081 gagagcaacg ctctcgggga attagtacag acgagtgcgg cgtgcgtttt cgcgagccag
     4141 tttcttcgcg tgacgtttca ctgcagaagc tttagcgcgc ttacgttcgg tagtcggttt
     4201 ttcatagaac tcacgacgac gaacttccgc cagaacacct gctttttcgc aggaacgctt
     4261 gaagcgacgc agagctacgt cgaacggctc gttttcacgt actttaatta ccggcatgtg
     4321 cctctcacct ttgattaatt cggtttgccg ctggcatcaa cgccagctta tttcaaaatg
     4381 gtgcggaatt ttactgcaat tgctgctgct ttgtaaagca ccgcggcctt ttttgaaagg
     4441 gacttttata agggtgagga gtatacacga ggctttctcc aggggcgaac aaagttttac
     4501 atcaacccgc attggtccta cactgcgcgg taataaagcg aggtaaaaca agtcatgcgt
     4561 gtactgggta ttgaaacttc ctgcgatgaa accggcatcg ccatttacga cgatgaaaaa
     4621 ggtttgttag ccaaccaatt gtatagtcag gtgaaattgc acgctgacta cggcggcgtc
     4681 gtgcctgaac tggcctcccg cgatcatgtg cgtaaaaccg taccgttgat ccaggcggcg
     4741 ctaaaggagt ctggtttaac ggcaaaagac attgatgctg tggcctatac cgcaggccct
     4801 ggattagtcg gcgcactgct ggttggcgca accgtggggc gctctctggc gtttgcctgg
     4861 aacgttccgg caatcccggt acaccatatg gaagggcatc tgttagcgcc gatgctggaa
     4921 gataacccgc cggaatttcc gtttgtcgcg ctgctggtgt ccggcggtca tacgcagtta
     4981 atcagcgtga ctggcattgg tcagtacgag ctgctcggcg agtctatcga tgatgccgcc
     5041 ggtgaagcgt ttgataaaac cgcgaagctg ctggggctgg attatcctgg cggaccgtta
     5101 ctgtcgaaaa tggcggctca gggtactgcc gggcgctttg ttttcccgcg tccgatgacc
     5161 gaccgtccgg ggctggattt cagcttttct ggtctgaaaa cctttgcggc gaacacgatt
     5221 cgtgacaaca gcaccgacga ccagacgcgt gctgatatcg cccgcgcctt tgaagatgcg
     5281 gtggtcgata cgttgatgat taagtgtaag cgagcgttgg atcagacggg ctttaagcga
     5341 ctggtcatgg cgggcggcgt gagtgctaac cgcacgttac gggcgaagct ggctgaaatg
     5401 atgaaaaaac gccgcggcga agtgttctac gcgcgtccgg aattttgtac tgataacggc
     5461 gcgatgatcg cctatgccgg aatggtgcgg tttaaagcag gcgcgacggc ggatctcggc
     5521 gttagcgtgc gtccgcgctg gccgctggcg gagttaccgg ctgcgtaaaa ctattgggtg
     5581 ccggagagca atttccggca ccgtcctcac ttaaagcaac accacgggca tccacagtaa
     5641 gccggtaatc accagcaaca cgaggaatat cagcccaaaa atcgccccca gtcgccagta
     5701 atccgccgtt ggcagataac cactaccgta gtaaatcgga ctgggtccgg tggcgtatgg
     5761 tgtaagaatg ctccccagac caattgccgc gccaaccatc aggcagaaca ccggcagcgg
     5821 gatttccggc atcgccagcg cggcggcgat catcatcggt gcgagggcgg aggtatacgc
     5881 cgtggcgctg gcgaaaaagt agcgcagtag ataaaacacc acaatcaacg tcaccatcac
     5941 catcgtcggc gaataaccac ttaagctgcc tgctaacagt ttgccaaacc agctaataaa
     6001 accggtattg ttgagtccgg tcgcgagggt gataagcgag gccagccaga agaaaacgtt
     6061 ccatgccgct ttattactga caatgtcgtc ccaactgata atgcgcagaa gcagcatcag
     6121 cgcgcctacc atcagcccca gcatccgttt ttcacgcgaa cagagcgggc ccattgcctg
     6181 cagttccgtc tctgcccagc gcggcacctg atcaccagac ttcagtaccg gcgggtatag
     6241 cacgtaagcc agccagggaa ccagcagaag cagtaaaatg ctgagcggca acatcccgag
     6301 gaaccagtcg ccccaactca gcgtggcgtg agatgcgctt ttcatcagtc caattaacag
     6361 caagttaggt gccatcgccg tcaggaaaat ggcgctggtc acgcagtcgg caacaatccc
     6421 catccacatg atgtacgagc caatgctgcg cgaactgctg tcgtttggtt gtgattgata
     6481 gagcggtggc aggttacgga tgatgggata gataataccc gcaccacgcg ccgagttgga
     6541 cggtgttaca ggtgccagga tcagctcgga gaacatcacc gcatagccga gaaacagcgt
     6601 gcgatgcccc atctttttca ccagaatcag cgcaatgcgg cgtccaagcc cggttttttc
     6661 atagcctgtg ccaaacataa aggcggcgaa aatgagccag ataaccgaat tagaaaaacc
     6721 agaaactgcc caggagaggg atttggcggt gaacttaaag cctggctggg cgagctgttc
     6781 ggggctgaac agcagccagg gagagagtat ggcgatgatg gagataccca ccatcgccac
     6841 cacggcaccc ggcacgggtt cgaggatcag tccaacgatc acgccagtaa aaacggcaaa
     6901 gtagagccag gtatggttct ccagccctgc gggaaccgga agtagagcaa taatggcgat
     6961 gaccgccagc ggtgcaagat atcgccacca ttcagtggaa ggtttcatcg tgttatgcct
     7021 gcgaaaaagg agaggggtga gcctctccgg gagggttatt tgatgtaatg gacatgctcg
     7081 cagatctctt ccacgatggg atcgcggcgt tcggcgaaca gctttttgtt ttcggctatc
     7141 aggttgttgc cgtgggtatc aatagagaca atcagcgggc cgaactcttt gacccggcag
     7201 acccacagtg actccggcat cccgagttct gtccagtgca cttcttcaat ctcttctacc
     7261 tgggttgccg ccagcaccgc gcagcctgcc gggaaaatca catgtaacgc cttgaatttc
     7321 tggcagcctt cttcggtcag cggccccata ccacctttgc caaccaccag ttttacgccg
     7381 gtctgctcaa taaattcacg ttcaaaactt tccatacgca tactggttgt cgggccgacg
     7441 gagaccatct cccatttgtc gccgtttttg cgcacgatgg ggccagcgtg gaaaatcgct
     7501 ttgccgttga gatcgtaagg gatcggacgt ttcagttcga tcaaacggcg gtgacaaacg
     7561 tcgcggcagg tcaccagcgt accggtcagg tagatcacat cgccgacgcg aatatcttgc
     7621 agatcttcag ctttgatcgg ggttgtcagg atctttttca taacgcgctc cgggtgtgag
     7681 acagattttc aaaggtgaga tcggcatgaa ccagcagcgt gccgcgacga tgcgcccatc
     7741 actgaactgt tgccggtcag cccttgtgga ccaatcccca gacggttgag tccttcttcc
     7801 aggcgtagct ccagttctgc cgcttttgga ttgggatggc ggctgccaat cgggcgcaga
     7861 atggctttac gcgagagtac ggcggcggtt tccaccgagg tggcgatgcc cacgcccacc
     7921 agtaccggtg gacaggcgtt tacggcgagg gtggagatat tttcgaagac gaatttcacc
     7981 acgccttcgt agccttctga cggcattaac actttcgagc ggccaggtag cgtgcagccg
     8041 ccgcctgcca tgtaaacttc gatttccgca tcgtcattgt cggggatgat gtcccaggtg
     8101 acccacggta cgccgctacc ggtatttttg ccggtgttta cttcgtcaaa aatttctacc
     8161 gcattgtgac gtagtggcgc tttgacggtt gcctcttcca cggcttgttt gagtatgctt
     8221 tgcagctcgc caagcagtgg gaagcgggaa ccgactttaa cgaagaacat aatctccccg
     8281 gtgtcctgac aggcaggacg attcaggtca atcgcttttt gcatgttgtc gaacatcgta
     8341 tggtagataa ttttccccat cgacgacgtt tcggcatcct ttagctgttt tagtttatcc
     8401 accacgtcat caggcattcg ggtagaaatc atggcggtaa agttagcgac aatctctgtc
     8461 aacttattca ctgcctgttg cttattactt tcgctcatca tggcaactcc agtttttgta
     8521 tttgtctgga aaaattcacc gccacaaact accactaaaa atgtggtttt atattttttg
     8581 ccaccgtgaa tatgtaatta acagttcgtg aatcaacgcg aaaataagaa tgaatattgc
     8641 gccagagcag ggaatggcaa tgttttgcga aatacactgc gcttatcaca actgcggata
     8701 attaacgatg ctgaatagct ggcctttagc caaagacttg caggtactgg tagaaatcgt
     8761 ccacagcggg agttttagtg ctgcagcggc gacgcttggg caaacgcctg catttgtcac
     8821 caagcggatc caaatccttg aaaatacgct ggcaacaacg ctgctgaacc gctccgctcg
     8881 cggcgtggcg ctgactgaaa gtggccaacg ttgttacgaa catgcgctgg aaatcctcac
     8941 ccaatatcag cgactggtcg atgacgtcac gcaaatcaaa acgcgcccgg aggggatgat
     9001 tcgtattggt tgcagttttg gttttgggcg cagccatatt gcgccagcca ttaccgaact
     9061 tatgcgcaat tatcctgagt tacaggtgca ttttgaattg ttcgatcggc aaattgattt
     9121 agtgcaggat aatattgatc tggatattcg tattaatgac gaaattcctg attattatat
     9181 tgcgcatctg ttaacgaaaa ataaaagaat attatgcgca gcacctgagt atctgcaaaa
     9241 atatccgcag ccacaatcat tacaggaatt aagtcgtcat gactgtctgg tgaccaaaga
     9301 gcgcgatatg acccacggaa tatgggagtt ggggaacggt caggagaaaa aatcggtgaa
     9361 ggttagcggg catctttcct ccaatagcgg cgagatagtg ttgcaatggg cgctggaggg
     9421 caaagggatt atgctgcgtt ccgagtggga tgtgttgccg tttctggaga gtggcaaact
     9481 ggtgcaagta ttgccggagt atgcacaaag cgccaatatc tgggctgttt accgggagcc
     9541 gctctatcgc agcatgaaat tcgtgtctgc gtggaatttc tggcggcatg gtgccagcaa
     9601 cggctgggca agcccgatga aggctatcag gtcatgtaga tccaccagaa atcactcggg
     9661 atccttttcg cgctttcttt tgaatttcgt ccagattttt gtctcctgac gacgccacag
     9721 acgctggatg ttgtcatgat gacgcagcag gatcaggcaa gagagcatcg aaaccgggaa
     9781 ggtgaattgt ggcttaaacc accagacata aaacggagca atcagtgcac tgacaatcgc
     9841 tcccagcgac gagtatccgc tcaatagcac ggtcagtaac caggttcccg ccattactcc
     9901 ggtgagatcc cagccaattg gtgcgattgc tccaaaagcg gtagcaacgc cttttcctcc
     9961 tttaaaaccg aagaaaacgg gccagatgtg tccaagacag gcggcgatgg caattaagcc
    10021 tagccagaag gggctgacac ctaattcata cgcgccccag acaggcaaca ttcctttcag
    10081 aacgtcgaaa atcagtactg ctacggctgc tcccttgcca ccgatacgta acacattggt
    10141 tgcgcctgga ttgccggagc cgctggttcg cggatcgggc agcccacaca agcggcaaac
    10201 cagaatggca ctggaaatgg agccgcagag gtacgcgatg aggatcattc caggcgcgat
    10261 tgcactcata agctgttccg ttttgaaaat tcgtgttcaa cgatgaatct gtggataata
    10321 cgcacatttc gccggaagtg gtatccggtt agccaaaaag caggcaggac gtgatggata
    10381 ttgtatttat agagcaactt tcggtaatca ccactattgg tgtttacgac tgggaacaga
    10441 ccatcgaaca gaagttagtg ttcgatatcg aaatggcatg ggataaccgt aaagcggcga
    10501 aaagtgatga tgtggcggat tgcctcagtt acgctgacat tgcagaaacg gtggtcagcc
    10561 acgtcgaggg ggcgcgtttt gcgctggtgg aacgcgtggc tgaagaggtg gcggagctgc
    10621 tgttagcacg cttcaactcg ccgtgggtgc gtatcaaact cagcaagcca ggcgcagtgg
    10681 cgcgggcggc gaatgttggc gtaatcattg agcgtggcaa taatctgaaa gaaaataatt
    10741 aattttacag ctgttaaacc aaacggttat aacctggtca tactgcagta gttcggacaa
    10801 gcggtacatt ttaataattt aggggtttat tgatgagcga tatgcactcg ctgctgatag
    10861 cggcaatatt gggtgtggtc gaaggattga cagaatttct gccggtatcc agcacgggcc
    10921 atatgattat tgtcggtcac ttgttggggt ttgagggcga cacggcgaaa acctttgaag
    10981 ttgtgatcca gttaggatca attctggcgg tagtagtgat gttctggcgg cgtctgtttg
    11041 gcctgattgg catccacttt ggccgcccgt tgcagcacga aggtgaaagc aaaggtcgtt
    11101 taacgctgat ccacattttg ctggggatga ttccggcggt ggtactgggg ctattattcc
    11161 acgacacgat taagtcattg tttaacccga taaatgtgat gtatgcgctg gtcgttggcg
    11221 gtttgttgct gattgccgcc gaatgcctga agccgaaaga gccgcgtgcg ccgggtcttg
    11281 atgatatgac ctatcgtcag gcgtttatga ttggctgttt ccagtgtctg gcgctgtggc
    11341 cgggtttctc ccgttccggg gcgaccattt caggtgggat gctgatgggc gtgagccgtt
    11401 acgctgcttc cgagttctcg ttcctgctgg cggtaccgat gatgatgggc gcaacggcgc
    11461 tcgatctcta caaaagctgg ggcttcctga caaccggcga tatcccgatg tttgccgttg
    11521 ggtttatcac cgcttttgtg gtggcgctga tagcgattaa aaccttcctg caattgatta
    11581 agcgcatttc gtttatcccg ttcgccattt atcgctttat tgtggcggct gcggtgtatg
    11641 tcgtgttctt ttgattgaga aaaggttcgg gagcaggccg ggttttctcc ctcgctctgt
    11701 agggagaggg ccggggtggg ggtgattgtt cgcaccggtg ctggtctgtt cccctcaccc
    11761 taaccctctc cccgaagggg cgaggggacg gttcgtgcta acgtttagtt ttatactcag
    11821 tcaggctttg ggcaacgttg ttccttccag ctggctaccg ccgcaatccg ccgtcgggtc
    11881 agctcctcgc gaatctccac gcctttaaat cccgcttcaa cgaccgcttt tgtcggcact
    11941 gactgcgcca cttcccagcc ttcgcgcaac caacggcctt gcgggtagtc cgctgattca
    12001 aaaccggttc tgccgcgcac gtcagcctcg ctggtcagcg ccagttgctc gacacgctgc
    12061 ggtttacgcc aggcatcgat ggaatcaaat aatttgacga tggtttttgg gttcagcatt
    12121 gggaaggtgt ggatgagatc gtgaaactca gccaccagtc tggctaaatc gcgaatttca
    12181 tttggcacgc gcagacgctg gcatagttgt tccactaact taacacccgc cgggccatgc
    12241 ccatgatgac gcggccaaag ttctggcggc gtcagtcctt taccgagatc gtggcataaa
    12301 gttgcgaaac ggacatcaac ctgcggactc agcatcgccg ccattgagag cgtcattaag
    12361 gtatgaatac ccgtgtcgat ttccggatgc cacttggcag gtgccggaac gccaaacagt
    12421 gcgtcaattt ccgggaataa aacgcgaagt gcgccgcaat cgcgcagtac ctggaagaac
    12481 acctggggat tgcgggtggt gagggcgctt tccgtctctt tccatacccg ttcaggcgtc
    12541 aggtgttcca gttcacccgc atgggtcatc tcgcgcatca gcgtcagagt ttcatcggca
    12601 atacgaaaac cgaggtgggc ataacgcgca gcaaaacgcg ccacgcgcag tacgcgtaac
    12661 ggatcttcgc caaaagcggg ggaaacatgg cgcaacagac gattttgcag atcgcccaga
    12721 ccgttgtacg ggtcgataat ctcgccgtta tcgtcctggg ccagcgcatt aatggtcaga
    12781 tcgcgacgct taagatcatc ttccagcgtg acatccggtg cggcatagca agtaaaaccg
    12841 gtgtaaccgg aaccggattt ccgttcggta cgtgccagcg catactcttc atgcgtttgc
    12901 ggatgcagaa acacaggaaa atcgcggcct acctgctggt agcccgcgtc gagcatctcc
    12961 tgtggcgtac tgccgaccac cacccaatct ctgtctttga ccgatagccc taacaatgca
    13021 tcccgaacag caccaccgac cagataaatc ttcacgccat catcccttcg tcaaatacac
    13081 aattcctaat aataagacag tgcaacgaag aaggcgattt agttcatcca gcgatctttg
    13141 cgtttgcggc ttgggatcag gtgcggcagt accagaccga gcagcaagcc aagccccagc
    13201 acgccaccgc catacataaa ccattgcatg atgatggtgc gctgtttgtc atccagctgt
    13261 acgctggcgg catcgacctt tttctgcgcg acaatcagct cgtttttcag tttctgattt
    13321 tcttctttta acccgttgat cacgctgtcg ctctgcgcca ctttttgctg catttctgcg
    13381 gtgcgctgat tccaggtgtt atcgatattg gtgagtttat cggtcagggt tttgacctga
    13441 ttttccagat ctggcacacg ggagcgcagg cttggctcag tgctaagttg tttcaacggg
    13501 atccaggcgg tacggccaga gctgtctttc acctgggcat aattggtgtt ggcgtcagtt
    13561 tgtaataagg tcacttcctc gccggcgtta accgtgccca cgaggcgata atgatctccc
    13621 ggaccgctac ggacccaggt attcagttcg tcagaaacat agcgcgtttc ttcagcgtgt
    13681 gagacggcag tcgcgctaag tgcgagtaaa gttaatccga tcaggcgtaa ttttggcatc
    13741 aggctgtcgt tattgtcatg gaaagtggaa cgatagtagt ggcatcagtg tcgctacgca
    13801 aagcattcga catcaatcgg aatcctctgt gtcaggtctt gccactgctt caaactttta
    13861 cgcccgtcaa attgcccgtt gcatggcaat ttggcgcaaa atactatcta ctgacaaaaa
    13921 agatcgcatt aagttcgccg ttcatcgtca cttatgtgac ataaccgata agtaaaggcc
    13981 atggctcagg aaatcgaatt aaagtttatt gttaatcaca gtgccgttga ggcgttgcgt
    14041 gaccatctca atacgctggg cggcgagcac catgaccccg tgcagttgct gaatatttac
    14101 tacgaaacgc cggataactg gctgcgtagg cacgatatgg gcttacgtat tcgtggcgaa
    14161 aacggtcgct atgagatgac catgaaagtt gcaggaagag tgacaggcgg cttacaccag
    14221 cgcccggaat ataacgtggc gttgagcgaa ccgacgctcg acctggcgca gttaccgacg
    14281 gaaatctggc cgaacggcga attgcctgcc gatctcgctt cccgcgtgca gccgctgttt
    14341 agcaccgatt tttatcgcga aaaatggcta gtagagatcg atggcagccg aattgaaatc
    14401 gctctcgacc agggggaagt gaaagcgggt gaatttgctg agcctatctg cgagctggaa
    14461 ctggagctgc ttagcggcga cacgcgcgcg gtgctgaaac tggcgaacca actggtatcg
    14521 caaaccggat tacgccaggg cagcctgagc aaagcggcgc gtggctatca tctggcgcag
    14581 ggcaatccgg cgcgtgaaat caaaccgacc accattttgc atgttgcggc aaaagccgat
    14641 gtggaacagg ggctggaagc ggcgctcgag ctggtgttag cgcaatggca gtatcatgaa
    14701 gaactgtggg tacgcggcaa cgatgcggcg aaagagcagg tgctggcagc cattagccta
    14761 gtccgtcata cactgatgct gttcggtggc attgtgccgc gtaaagcgag cactcactta
    14821 cgtgatttgc tgactcaatg cgaagcgacc atcgcttctg cggtttctgc cgtgacggcg
    14881 gtctactcta ccgaaacggc aatggcgaag ctggcgttga ccgaatggct ggtaagcaaa
    14941 gcatggcagc cgtttttaga tgccaaagcg cagggcaaaa ttagcgactc cttcaaacgc
    15001 tttgccgata tccatctttc ccgccatgcc gctgaactga aaagcgtttt ctgccagccg
    15061 ttaggcgatc gctaccgtga ccagttgcca cgcctgatgc gtgatattga ctcaatactg
    15121 ttgctggcgg gttactatga tcctgtcgtc gcgcaagcct ggctggagaa ctggcagggg
    15181 ctgcgtcacg ctattgcgac cgggcaacgc attgaaattg aacatttccg taatgaggca
    15241 aacaatcagg aaccgttctg gttgcacagc ggaaaacgtt aatcaggcaa ggataaaatc
    15301 gcttatgaag ccgctctctt caccgttaca gcagtactgg cagaccgttg ttgagcggct
    15361 gccagagctt ttagccgagg aatcacttag tgcacaggcg aagtcagtac ttactttcag
    15421 tgattttgtg caggacagcg tg
//
LOCUS       DADLTF010000096        15410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.096, whole genome shotgun sequence.
ACCESSION   DADLTF010000096 DADLTF010000000
VERSION     DADLTF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.096"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1175)
                     /locus_tag="JN749_002783"
     CDS             complement(<1..1175)
                     /locus_tag="JN749_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7369143.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRL"
     gene            complement(1374..1458)
                     /locus_tag="JN749_002784"
     tRNA            complement(1374..1458)
                     /locus_tag="JN749_002784"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1422..1424),aa:Leu,seq:caa)
     gene            1654..2673
                     /gene="ahr"
                     /locus_tag="JN749_002785"
     CDS             1654..2673
                     /gene="ahr"
                     /locus_tag="JN749_002785"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HAY7369144.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2802..4304
                     /locus_tag="JN749_002786"
     CDS             2802..4304
                     /locus_tag="JN749_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HAY7369145.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="JN749_002787"
     CDS             complement(4423..5505)
                     /gene="lptG"
                     /locus_tag="JN749_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HAY7369146.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="JN749_002788"
     CDS             complement(5505..6605)
                     /gene="lptF"
                     /locus_tag="JN749_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HAY7369147.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPIRRLRASFSRKGAV"
     gene            6872..8383
                     /gene="pepA"
                     /locus_tag="JN749_002789"
     CDS             6872..8383
                     /gene="pepA"
                     /locus_tag="JN749_002789"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HAY7369148.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRTGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8517..8960
                     /gene="holC"
                     /locus_tag="JN749_002790"
     CDS             8517..8960
                     /gene="holC"
                     /locus_tag="JN749_002790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HAY7369149.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8960..11815
                     /gene="valS"
                     /locus_tag="JN749_002791"
     CDS             8960..11815
                     /gene="valS"
                     /locus_tag="JN749_002791"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAY7369150.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11869..13065)
                     /locus_tag="JN749_002792"
     CDS             complement(11869..13065)
                     /locus_tag="JN749_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HAY7369151.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSAGMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNSVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILTFLDLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13258..13761
                     /locus_tag="JN749_002793"
     CDS             13258..13761
                     /locus_tag="JN749_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7369152.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="JN749_002794"
     CDS             complement(13807..14223)
                     /gene="rraB"
                     /locus_tag="JN749_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HAY7369153.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14385..15398
                     /gene="argF"
                     /locus_tag="JN749_002795"
     CDS             14385..15398
                     /gene="argF"
                     /locus_tag="JN749_002795"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAY7369154.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3972 a         3929 c         3969 g         3540 t
ORIGIN      
        1 atcaacctac gctccccaag atgttccgcc ttatggatgt aagctgctcg cacagagcta
       61 cgctcctggt gactcatctg ccgctctacc gcatccctcg accataatcc cgactcaatc
      121 aacgaactac aagccatcgt cctgaagcca tgcccacaaa cctcgacctt cgtgtcatac
      181 cccattacgc gcaaagcctt gttcaccgta ttctcactca tcggcttacg tggatcgtga
      241 tcgcccacaa aaatcagctc tcgatttccg ctcatgcttt tgatcttttc caaaatactt
      301 agggcctgcc gcgacaaagg tacaaggtgt ggtgtccgca tcttcgagcc acgctgagaa
      361 tgcttgactc cttccagttg ctcacgctca cctgggattg tccacatagc cgtttcaaaa
      421 tctacttctg accagcgggc aaaacgcagc tcgctcgaac ggatgaaaac caataaagtg
      481 agttcaaccg ccagtcgagt taacggtctg ccagaatagt gatctatgcg atgaagtaat
      541 tcaggtatac ggttcagttc aagagctgca cggtgctgtc ttttcgccgt agcaaccgca
      601 cctgcaatct cttgcgcggg gttgtagtcg attaagccgc tctgaacggc aaagcgcata
      661 atcgcggtag tacgctgctg taaacgggcg gcaacttcga gccgcccgga tgactcgact
      721 gctttaatgg gtacaagaag atcccgcgtc ttcagttccg caatgttccg cttaccgata
      781 gcagtaaaga gattatcctc cagacttttc aaaacacgag cgctatgcga tgccgaccac
      841 ttctgattgc tggcgtgcca gtctctggcg actacttcaa acgttatcgc ctcttgttcc
      901 tgttctacct taacagcttt cttgttttca ctgggatcga caccattagc taaaagctta
      961 cgagcctcat cccggcgtgc cctggcatcc gccaacgaca cttcggggta tttccccaat
     1021 gcaagcatct tctctttgcc gccaaagcga taacgtagcc gccagtattt ggagccgtta
     1081 gggtgaacca gcaaaaccat gccttcaccg tcggtcagct tataggcttt tgcttcaggc
     1141 ttagccgaac gaaccttcac atcactcaga gccatgatga gtatcctttc aagggttctg
     1201 tgtgggtaca aacattatcg aaccgggaaa tacccgcagt tgtacccgca tcagtaagtt
     1261 gatgtagatt gaatcaggtt gacttaggtt gagtgaaaaa gcgaggaaag ccttgcggat
     1321 actggatttt aggcacaaaa aaagacgtcc gttgacgtct attgatgttc cgatggtgcc
     1381 gaaggccgga ctcgaaccgg cacgtatttc tacggttgat tttgaatcaa ctgcgtctac
     1441 cgatttcgcc acttcggcac tgaagaggta tgcggaaaac gttgtggatt atacctgtcg
     1501 cacgccacca tgcaagcgcc agcttgcacc cacctcgcta agtgctgaaa ttttcagcat
     1561 tacactcagc gatatgtaac ctttgtcaca ctccaggcac cccgccctgc catgctctac
     1621 acttcccaaa caacaccaga gaaggaccca aaaatgtcga tgataaaaag ctatgccgca
     1681 aaagaagcgg gcggcgaact ggaagtttat gagtacgatc ccggtgagct gaagccacaa
     1741 gatgttgaag tgcaggtgga ttactgcggg atttgtcatt ccgatctgtc gatgatcgat
     1801 aacgaatggg gattttcaca atatccgctg gttgccgggc atgaggtgat tgggcgcgtg
     1861 gtggcactcg ggagcgccgc gcaggataaa ggtttgcagg tcggtcagcg tgtcgggatt
     1921 ggctggacgg cgcgtagctg tggtcactgc gacgcctgta ttagcggtaa tcagatcaac
     1981 tgcgagcaag gtgcggtgcc gacaattatg aatcgcggtg gctttgccga gaagttgcgt
     2041 gcggactggc aatgggtgat tccactgcca gaaaatattg atatcgagtc cgccgggccg
     2101 ctgttgtgcg gcggtatcac ggtctttaaa ccactgttga tgcaccatat cactgctacc
     2161 agccgcgttg gggtaattgg tattggcggg ctggggcata tcgctataaa acttctgcat
     2221 gcaatgggat gcgaggtgac ggcctttagt tctaatccgg cgaaagagca ggaagtactg
     2281 gcgatgggtg ccgataaagt ggtgaatagc cgcgatccgc aggcactgaa agccctgtcg
     2341 gggcagtttg atctcattat caatactgtg aacgtcagcc tcgactggca gccttatttt
     2401 gaggcgctga cctatggcgg taatttccat acggtcggtg cggttctcac gccgctgtct
     2461 gttccggcct ttacgttaat tgcgggcgac cgcagcatct ctggttctgc taccggcacg
     2521 ccttatgagc tgcgaaagct gatgcgcttt gccgcccgca gcaaggttgc gccgacaacc
     2581 gaactgttcc cgatgtcgaa aattaacgac gccattcagc atgtacgcga tggtaaagcg
     2641 cgctaccgag tagtcctgaa agccgacttc tgatcccttt gccgggtgct gtcgcccggc
     2701 acttaaatct ctttggaagg cgtctcgcaa tctgctgaat ttagcggtga agagtgcaca
     2761 gccgttgcct atactccagg caggaaactg gaggaaatct catgagcgaa cccctgttaa
     2821 ttgcccgcac gccggacaca gaactgtttt tactgccggg aatggctaac cgtcacgggc
     2881 tgattactgg cgcaacgggg acgggtaaaa ccgttacgct gcaaaaactg gcagagtcat
     2941 tgtcggaaat cggcgtgccg gtgtttatgg ctgatgtgaa aggcgatctg accggtatcg
     3001 cgcaggcagg aacggcgtcg gaaaaactgc tcacaaggct taaaaatatc ggcgtcaatg
     3061 actggcaacc gcatgccaat ccggtggtgg tgtgggatat ctttggcgag aaaggccaca
     3121 cggtgcgggc gacggtttcg gatctggggc cgctgttgct ggcgcgactg ttgaatctca
     3181 acgatgtgca atctggcgtg ctgaatatta tcttccgcat tgcggacgat cagggattgt
     3241 tgctgctcga ctttaaagat ttgcgggcga ttacccagta catcggcgat aacgccaaat
     3301 ccttccagaa tcagtacgga aatatcagta gcgcatcggt tggtgccatc cagcgcggat
     3361 tactgtcgct ggaacagcaa ggcgcagcac acttctttgg tgagccgatg ctggatatca
     3421 aagactggat gcgcaccgat gccaacggta aaggcgttat caatatcctc agcgccgaga
     3481 aactttatca gatgccgaaa ctgtacgccg ccagcctgct gtggatgctt tcagagttgt
     3541 atgaacaatt gccggaagcg ggcgatctgg agaaaccaaa actggtgttt ttcttcgacg
     3601 aggcacatct gctgtttaac gatgcaccgc aggtactgct ggataagatt gagcaggtga
     3661 taaggcttat tcgctcaaaa ggcgtgggcg tctggttcgt ttcgcaaaac ccgtctgata
     3721 ttccggataa tgtgctcggc cagctcggta atcgtgttca gcacgctttg cgagctttta
     3781 cgccaaaaga tcagaaagca gtgaagacag cggcgcaaac catgcgggcc aatccggcat
     3841 ttgataccga aaaggcaatc caggaactgg ggaccggcga ggcgttaatc tcgtttctcg
     3901 atgcaaaagg aagtccttct gtggtggaac gggcgatggt gatcgcacct tgttcgcgaa
     3961 tggggccggt gacggaagat gagcgtaatg gcctgattaa tcactccccg gtgtatggca
     4021 aatatgaaga tgaggtggac cgcgagtccg cctatgagat gctgcaaaaa ggctttcagg
     4081 ccagtaccga gcagcaaaat aatccccccg tgaaagggaa agaggtggcg gtggatgacg
     4141 gtattcttgg tggattgaag gatattttgt ttggcactac cggaccacgc ggcgggaaga
     4201 aagatggtgt ggtgcaaaca atggcgaaaa gcgccgctcg ccaggtgacg aatcagattg
     4261 tacgcgggat gttggggagt ttgctggggg ggagaaaaag ataagtcaga cgtgatctgt
     4321 gcccctcacc ctaaccctct ccccaaaggg gcgaggggac cgttcgaact cgtatttgac
     4381 tttgtcaaca ataataaagg gagctttcgc tcccttgtta gattacgatt ttctcattaa
     4441 cagccacagg ctgattaaga agaagctggc acttggcaac agtgcgccga tgatcggcgg
     4501 gatgccgtaa accaacgtca gcgggccgaa gatctggtcc agtacgtaga agacaaaacc
     4561 gaagctgata cctgtgacca cacgcacgcc catcggtacg ctacgcagtg ggccaaagat
     4621 aaacgacagc gccatcagca tcatcaccgc cacggatagc ggctggaaga ttttgctcca
     4681 catgttgagc tgataacgtc cggcatcctg accgctcgac ttcagatact tcacgtagtt
     4741 gtgcaaacca ctgatggaga gtgcatccgg gtccagcgcc accacgccca gtttgtctgg
     4801 cgtgaggtcg gttttccagg tgccgctcac cgtctgcgaa ccggtaatct gtttcggatt
     4861 ggtcagatca gattcatcaa cctgcgacag acgccagact ttatgttccg ggtcaaactt
     4921 cgcggtagcg gcataacgta cggattgcag acgacgattc tcgttaaagg cataaatact
     4981 gatgccacct aacacctcgt cacctttaac ccgctcaatg tagacgaagt tgttgccatc
     5041 tttcgcccat aaaccttgct gggtagagag caacgagccg ccgtacatcg cctgcgcacg
     5101 gtagttacgc gccatctgtt cgccctgcgg cgcgacccat tcgccaatcg ccatcgtcag
     5161 caagaccagt ggaatggcgg ttttcatcac cgacagcgct acctgcatac gggtaaaacc
     5221 agaagcctgc atcaccacca gttcgctgcg ctgcgccagc atcccaagac caagcaacgc
     5281 cccaagaaga gccgccatcg ggaagaagat ctgcacatct ttcggcacgc tcagcaaggt
     5341 atacattcct gcgcctaacg cgtcgtaact cccctgcccg gcttttttca gctgatcgac
     5401 aaatttgata atgcccgaca gcgacaccag catgaacagc gtcatcatga tggtggtgaa
     5461 aatagtttta ccgatatagc ggtcaagtac gccaaaaggt tgcatcacac cgctccttta
     5521 cgcgaaaaac tggcgcgcag gcggcggatc ggcacggtgt cccaaaggtt gagaacaatc
     5581 gccaaagcca gataaatcag gttaacggtc cacatccaca gtgtcgggtc cagcttacct
     5641 ttaccgccgt tcgatttcag ggaggtctgg atcaggaaga aaagtagata cagcagcatg
     5701 gctggcagca tcgacagtac gcgtccctga cgcgggttaa ccacgctcaa cggtacaacc
     5761 ataagtgcca tcataaacac ggtgaatacc aacgtgatac gccagttcag ttctgcgcga
     5821 gcacgatcgg tgtcagtgtt ccacaatgtg cgcatgtcca tctggtcggt atcgttcggg
     5881 tcgagcgcca ccgcctggtg accaatgatc gcctgataat cctggaaatc cgtaatgcgg
     5941 aaatcacgta acagtgcagt gccttcgaag cgtgttccct ggttgagagt gacgacctgg
     6001 gagccgtcgc gcagctgggt taaatgtccg gaatcggcca ccaccacaga aggacgtgca
     6061 ttaccttttg gtcgaatttg tgcgaggaac acatctttga aatcgctgcc gtcaacgctt
     6121 tcgatgaaca gcaccgagct gccattagtc gcttgctgga attgcccttg cgccagcgcc
     6181 gccatgccag ggttcgcttt cgcttctgct aacacttcat cctgatgacg cgatgaccac
     6241 ggtcccgccc acatcacgtt aaccgccgcg acgattgccg tgaataccgc aaggatcatt
     6301 gccgctttca ccagaaccgc tttgctcaag ccgcaggcat gcattaccgt aatttcactt
     6361 tcggtataca gtttgcccag cgtcatcagc agcccgagga acaggcttaa tggcaggata
     6421 agctgcgcca tttccggcac gcccaacccg agaagggaga gcaccagatt cgccggaata
     6481 tcgccgtcaa ccgctgcgcc gaggatcctc actaactttt gacagaagaa gatcaaaagc
     6541 aagatgaaga gtatcgccag ctggcttttg agcgtctccc gcaccagata tcttatgatt
     6601 atcactttaa atacgcccgt aaaaactcgt cttttgcagg attttagctt gtttcatggc
     6661 ttaaacgtca tttattctct tgagtcgtcg aaatcgtcgc taagataatt atactcaacg
     6721 gattcacctc tcagattttg ttctgacgtg ccaatgccgt aataacgtta agattaacac
     6781 gaagtcatcg caacagcgga catgagttac gaaagcttgc aattctatct gtagccaccg
     6841 ccgttgtctt taagattcag gagcgtagtg catggagttt agtgtaaaaa gcggtagccc
     6901 ggagaaacag cggagtgcct gcatcgtcgt gggcgtcttc gaaccacgtc gcctttctcc
     6961 gattgcagaa cagctcgata aaatcagcga tgggtacatc agcgccctgc tacgccgggg
     7021 cgaactggaa ggaaaaccgg ggcagacatt gttgctgcac catgttccga atgtactttc
     7081 cgagcgaatt ctccttattg gttgcggcaa agaacgtgag ctggatgagc gtcagtacaa
     7141 gcaggttatt cagaaaacca ttaatacgct gaatgatact ggctcgatgg aagcggtctg
     7201 ctttctgact gaactgcacg ttaaaggccg taacaactac tggaaagtgc gtcaggctgt
     7261 cgagacagca aaagagacgc tctacagttt cgatcagttg aaaacgaata agagcgaacc
     7321 gcgtcgtccg ctgcgtaaaa tggtgttcaa cgtgccgacc cgccgtgaac tgaccagcgg
     7381 tgagcgcgcg atccagcacg gtctggcgat tgccgccggg ataaaagcag cgaaagatct
     7441 cggtaatatg ccgccgaata tctgtaacgc cgcttatctc gcctctcagg cgcgccagct
     7501 ggctgacagc tacagcaaga atgtcatcac ccgcgttatc ggcgaacagc agatgaaaga
     7561 gctgggaatg cactcttatc tggcggtcgg tcagggttca caaaacgaat cgctgatgtc
     7621 ggtgattgag tacaaaggca acgcgtcgga agatgcacgc ccaatcgtgc tggtgggtaa
     7681 aggtttaacc ttcgactccg gcggtatctc catcaagcct tcagaaggca tggatgagat
     7741 gaagtacgat atgtgcggcg cggcggcggt ttacggcgtg atgcgtatgg tcgcggagct
     7801 gcaactgccg attaacgtta ttggcgtgct ggcaggctgt gaaaacatgc ctggcgggcg
     7861 tgcctatcgt ccgggcgatg tgttaaccac catgtccggt caaaccgttg aagtgctgaa
     7921 caccgatgcc gaaggccgcc tggtactgtg cgacgtgtta acttacgttg aacgttttga
     7981 gccggaagca gtgattgacg tggcgacgct gaccggtgcc tgcgtgatcg cgctgggtca
     8041 tcatattact ggtctgatgg cgaaccataa tccgctggcc catgaactga ttgccgcgtc
     8101 tgaacaatcc ggtgaccgcg catggcgctt accgctgggt gacgagtatc aggaacagct
     8161 ggagtccaat tttgccgata tggcgaacat tggcggtcgt actggtgggg cgattaccgc
     8221 aggttgcttc ctgtcacgct ttacccgtaa gtataactgg gcgcacctgg atattgcagg
     8281 aaccgcctgg cgttctggta aagcaaaagg cgcaaccggt cgtccggtag cgttgctggc
     8341 acagttcctg ttaaaccgcg ctgggtttaa cggcgaagag taagtttgcg ttttaccctc
     8401 accctaaccc tctccctaag ggagagggaa ccgatcgagc gcaatagaaa ttcattgctc
     8461 atctttcagg gtgaggaatg cagcaatatt taaatccacc acaagaagcc ccatatatga
     8521 aaaacgcaac gttctacctt ctggacaatg acaccaccgt cgatggctta agcgccgttg
     8581 agcaactggt gtgtgaaatt gccgcagaac gttggcgcag cggtaagcgc gtactcatcg
     8641 cctgtgaaga tgaaaagcag gcttaccggc tggatgaagc cctgtgggcg cgtccggcag
     8701 aaagctttgt tccgcataat ttagcgggag aaggaccgcg cggcggtgcg ccggtggaga
     8761 tcgcctggcc gcaaaaacgc agcagcagcc cgcgcgatat attgattagt ctgcgaacaa
     8821 gctttgcaga ttttgccacc gctttcacag aagtggtaga cttcgttcct tatgaagatt
     8881 ctctgaaaca actggcgcgc gaacgctata aagcctaccg cgtggctggt ttcaacctga
     8941 atacggcaac ctggaaataa tggaaaagac atacaaccca caagatatcg aacagccgct
     9001 ttacgagcac tgggaaaagc agggctactt taagcctaat ggcgatgaaa gccaggaaag
     9061 tttctgcatc atgatcccgc cgccgaacgt caccggcagt ttgcatatgg gtcacgcctt
     9121 ccagcaaacc atcatggata ccatgatccg ctatcagcgc atgcagggca aaaacaccct
     9181 gtggcaggtc ggtactgacc acgccgggat cgctacccag atggtcgttg agcgcaagat
     9241 tgccgcagaa gaaggtaaaa cccgtcacga ctacggccgc gaagctttca tcgacaaaat
     9301 ctgggaatgg aaagcggaat ctggcggcac cattacccgt cagatgcgcc gtctcggcaa
     9361 ctccgtcgac tgggagcgtg aacgcttcac catggacgaa ggcctgtcca atgcggtgaa
     9421 agaagttttc gttcgtctgt ataaagaaga cctgatttac cgtggcaagc gcctggtaaa
     9481 ctgggacccg aaactgcgta ccgctatttc tgacctggaa gtggaaaacc gcgaatcgaa
     9541 aggttcgatg tggcacatcc gctatccgct ggctgacggc gcgaaaaccg cagacggtaa
     9601 agattatctg gtggttgcga ccactcgccc ggaaaccctg ctgggcgata ccggtgtagc
     9661 tgttaacccg gaagatccgc gttacaaaga tctgattggc aaatatgtca ttctgccgct
     9721 ggttaaccgt cgtattccga tcgttggcga cgaacacgcc gacatggaaa aaggcaccgg
     9781 ctgcgtgaag atcaccccgg cgcacgactt taacgactat gaagtcggta aacgtcacgc
     9841 cctgccgatg atcaacatcc tgacctttga cggtgatatc cgtgaaagcg cccaggtgtt
     9901 cgataccaaa ggtaacgaat ctgacgttta ttccagcgag atcccggcag agttccagaa
     9961 actcgagcgt tttgctgcac gtaaagcagt cgttgctgcg gttgatgcac tcggcctgct
    10021 ggaagaaatt aaaccgcacg acctgaccgt tccttacggc gaccgtggcg gcgtggttat
    10081 cgaaccgatg ctgaccgacc agtggtacgt gcgtgccgat gtactggcga aaccggcagt
    10141 tgaagcggtt gagaacggcg acattcagtt cgtaccgaag cagtacgaaa acatgtactt
    10201 ctcctggatg cgcgatattc aggactggtg tatctctcgt cagctgtggt ggggtcaccg
    10261 tatcccggca tggtatgacg aagcgggtaa tgtttatgtt ggccgcaacg aagaagaagt
    10321 gcgcaaggaa aataacctcg gtgccaacgt tgccctgcgt caggacgaag acgttctcga
    10381 cacctggttc tcttctgcac tgtggacctt ctctaccctt ggctggccgg aaaataccga
    10441 cgccctgcgt cagttccacc caaccagcgt gatggtatct ggtttcgaca tcatcttctt
    10501 ctggattgcc cgcatgatca tgatgaccat gcacttcatc aaagatgaaa atggcaaacc
    10561 gcaggtgccg ttccacactg tttacatgac cggtctgatt cgtgacgacg aaggtcagaa
    10621 gatgtccaaa tccaagggta acgttatcga cccgctggat atggttgacg gtatttcgct
    10681 gccagaactg ctggaaaaac gcaccggcaa tatgatgcag ccgcagctgg cggacaaaat
    10741 ccgtaagcgc accgagaaac agttcccgaa cggcattgag ccgcacggca ccgatgccct
    10801 gcgcttcacc ctggcggcgc tggcgtctac cggtcgtgac atcaactggg atatgaagcg
    10861 tctggaaggc taccgtaact tctgtaacaa gctgtggaac gccagccgct ttgtgctgat
    10921 gaacacagaa ggtcaggatt gcggcttcaa cggcggcgaa atgacgctat cgctggcgga
    10981 ccgctggatt ctggcggagt tcaaccagac tatcaaagcg taccgcgaag cgctggacag
    11041 cttccgcttc gatatcgccg caggcatttt gtatgagttc acctggaacc agttctgcga
    11101 ctggtatctg gagctgacca agccggtaat gaacggtgga acagaagccg aactgcgcgg
    11161 tactcgccat acgctggtga ctgtactgga aggtctgctg cgcctcgcgc atccgatcat
    11221 tccgttcatc accgaaacca tctggcagcg tgtgaaagta ctttgcggta tcactgccga
    11281 caccatcatg ctgcagccgt tcccgcagta tgatgcatct caggttgatg aagccgcact
    11341 ggccgacacc gagtggctga aacaggcgat cgttgctgta cgtaacatcc gtgcagaaat
    11401 gaacatcgcg ccgggcaaac cgctggagct gctgctgcgt ggttgcagcg cggatgcaga
    11461 acgtcgcgta aatgaaaacc gtggcttcct gcaaacgctg gcgcgtctgg aaagcatcac
    11521 cgtgctgcct gccgatgaca aaggtccggt ttccgttacc aagatcatcg acggcgcaga
    11581 gctgctgatc ccgatggctg gcctcatcaa caaagaagat gagctggcgc gcctggcgaa
    11641 agaagtggcg aagatcgaag gtgaaatcag ccgcatcgag aacaagctgg caaatgaagg
    11701 ctttgttgcc cgcgcaccgg aagcggttat cgcgaaagag cgtgagaagc tggaaggtta
    11761 tgcagaagcg aaagccaagc tgattgaaca gcaggctgtt atcgccgcac tgtaatcaac
    11821 acagtgatga aaacgaaggc cggagcatgc tccggccttt tttatctctt acagaaatgg
    11881 taaagaaggc atgactcccc gagaaatact ggctaagaag cctttatctg gttgttcttc
    11941 tttattgatt aaaggaaggt catccaaatc gcccagcaaa tacgtatttt ttgcctgcca
    12001 gtcaatcatc catattttca gcagtgggta agccagacca cccgtaatcc cggatatcac
    12061 caccaacgca cacatgcgat aaagcatacc gtggtacgtt aaagttgagc gaaaacgaat
    12121 acgcccatca ttcagtgaca ggttgctcat aaaatgattt cgcaacgaca ccgttaaata
    12181 actggtgcta acagcaatcc caaaataata aattaactgc gcgattatca ttttacgttg
    12241 catttccata aattgctgta aattctcaat atcatcattt gcatacccag aactatcata
    12301 ctcatttaat atttgatcga agataatata accagcaaca gcgagaaaag gtaataaatc
    12361 taaaaatgtg aggatggcat attttataca atacgcagta tctaatttca ccttgaaacg
    12421 atgtataccg aaactggtat tgctccagag aaaactcatt accaggctat ataaagtacc
    12481 attaaaaata ccaatggaaa caataccaac tattgccatc agaaccacag atataataac
    12541 actacttgaa ctattggcat gtagcatctt tgtcgagata aagaaaacag tccccatccc
    12601 aatggccatt aaaatcggca agaaaaaggt tacccaccag aaccctttca aagagcaatt
    12661 aaaactaaat cttacgctgt tgagactggt cattaaggcc tgataacgca gtccttttgc
    12721 tgccataaat ataagcaaaa ccaacagtaa taaagtcaaa acacagccaa caagcggcat
    12781 acctgctgac actgtcatta agattgcgaa atagaaaaaa acaaaaacaa gacaactaac
    12841 aaaaacattc ccaccggtaa ttccataaga aaatcgttgt ccgttaactt ccatattagc
    12901 ataaagataa cgcttacatt tcattaatgc ccatggtaaa tagataccta gcgttataat
    12961 cgttaacaac acattcacca cacaaataag aaaatatctc tcacctgtac catgaaaaac
    13021 aaaagaatgg gacggaacat ccatttcatt aataacttga gccataaaaa tcccctgaat
    13081 taaaaaatcc attgatataa tttatagata cagacagtaa aatgttctgc cacgttacaa
    13141 tactcttatc agtaataggc tggcaaaaac tttattgctt tataagacgc ttgcgcacgt
    13201 aagaagagag tggtattaaa caaatgtata attactttat ttttattggg ttaaactatg
    13261 aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgcagga taatcctgct
    13321 atcgccagcg tcatccgtca ggtttacgca gagtatggtc ttaccgccga taaaggctac
    13381 accgttgccg atccgaatct cgacgaactg tatcaagtgt acagccagcc tggccatgct
    13441 tattgggtgg ttgaatacaa tggcgaagtg gtcggcggcg gcgggattgc gccattaaca
    13501 ggtagtgaag cggacatttg cgaactgcag aaaatgtact ttcttcccgc tattcgcggt
    13561 aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggctcgaga gatgggcttc
    13621 aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct ttatgagcat
    13681 ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga ttgtgaagtg
    13741 cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg cattttaatc
    13801 aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat cgtcgccatc
    13861 ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt caaatttctc
    13921 cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca acgcgcactc
    13981 gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct cttctggatc
    14041 ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag tttccagatc
    14101 gtctgcggaa agatggtgtt cgatggtgta cagcgcgtcc ggatcgctgc catcttccag
    14161 taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt ccgggtttgc
    14221 catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc actgacattg
    14281 cctccacctt tgtgacaaag atttatgctt tagacttgca aatgaataat catacacata
    14341 aatttaattt taattcattg aggcgttagc cacaggaggg atctatgtcc gggttttatc
    14401 ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc ctgctgcagt
    14461 tagccgcgaa gctgaaagct gataagaaaa gcggtaaaga agaagccaaa ctcactggta
    14521 aaaacatcgc gctcatcttc gaaaaagact cgactcgtac ccgatgctct ttcgaagttg
    14581 ccgcatatga ccagggtgct cgcgttactt atcttggccc aagcggtagt cagattggtc
    14641 ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac ggtattcagt
    14701 atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc gtgccggtat
    14761 ggaacggcct gaccaatgag tttcacccca cgcagttgct ggcggatctc ctcaccatgc
    14821 aggagcattt acccggtaaa gcattcaacg aaatgacgct ggtctatgca ggtgacgcac
    14881 gtaacaacat gggcaattcg atgctcgaag ctgcggcgct taccggtctg gatttgcgtc
    14941 tggtcgcgcc acaggcatgc tggccagaag ctgcgctggt tacggaatgc cgcgccctgg
    15001 cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga gttgaaggtg
    15061 ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagaa aaatgggctg
    15121 aacgcattgc attactgcgt gattatcagg tgaacagcaa gatgatgcag ttgaccggta
    15181 acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa acgacgcttg
    15241 gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact gatgaggtct
    15301 tcgagtctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat accatcaaag
    15361 cggtgatggt cgcgacgctc agtaaattaa ataattaata tctctataaa
//
LOCUS       DADLTF010000097        15027 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.097, whole genome shotgun sequence.
ACCESSION   DADLTF010000097 DADLTF010000000
VERSION     DADLTF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.097"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..236
                     /locus_tag="JN749_002796"
     CDS             <1..236
                     /locus_tag="JN749_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HAY7369155.1"
                     /translation="LGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPA
                     LSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            246..884
                     /locus_tag="JN749_002797"
     CDS             246..884
                     /locus_tag="JN749_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HAY7369156.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLGRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            887..2050
                     /locus_tag="JN749_002798"
     CDS             887..2050
                     /locus_tag="JN749_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HAY7369157.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2134..3758
                     /locus_tag="JN749_002799"
                     /pseudo
     CDS             2134..3758
                     /locus_tag="JN749_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="JN749_002800"
     CDS             complement(3875..4150)
                     /gene="yjfN"
                     /locus_tag="JN749_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HAY7369158.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="JN749_002801"
     CDS             complement(4299..4628)
                     /gene="bsmA"
                     /locus_tag="JN749_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HAY7369159.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4810..5559
                     /gene="yjfP"
                     /locus_tag="JN749_002802"
     CDS             4810..5559
                     /gene="yjfP"
                     /locus_tag="JN749_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAY7369160.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="JN749_002803"
     CDS             complement(5556..6311)
                     /gene="ulaR"
                     /locus_tag="JN749_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HAY7369161.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="JN749_002804"
     CDS             complement(6419..7483)
                     /gene="ulaG"
                     /locus_tag="JN749_002804"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAY7369162.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7838..9235
                     /gene="ulaA"
                     /locus_tag="JN749_002805"
     CDS             7838..9235
                     /gene="ulaA"
                     /locus_tag="JN749_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAY7369163.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9251..9556
                     /gene="ulaB"
                     /locus_tag="JN749_002806"
     CDS             9251..9556
                     /gene="ulaB"
                     /locus_tag="JN749_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HAY7369164.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9566..10030
                     /gene="ulaC"
                     /locus_tag="JN749_002807"
                     /pseudo
     CDS             9566..10030
                     /gene="ulaC"
                     /locus_tag="JN749_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
     gene            10044..10694
                     /gene="ulaD"
                     /locus_tag="JN749_002808"
     CDS             10044..10694
                     /gene="ulaD"
                     /locus_tag="JN749_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAY7369165.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10704..11540
                     /locus_tag="JN749_002809"
     CDS             10704..11540
                     /locus_tag="JN749_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAY7369166.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARMAKAGMVE
                     AA"
     gene            11540..12226
                     /locus_tag="JN749_002810"
     CDS             11540..12226
                     /locus_tag="JN749_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HAY7369167.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12355..12630)
                     /locus_tag="JN749_002811"
     CDS             complement(12355..12630)
                     /locus_tag="JN749_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7369168.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            12957..13352
                     /gene="rpsF"
                     /locus_tag="JN749_002812"
     CDS             12957..13352
                     /gene="rpsF"
                     /locus_tag="JN749_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAY7369169.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13359..13673
                     /gene="priB"
                     /locus_tag="JN749_002813"
     CDS             13359..13673
                     /gene="priB"
                     /locus_tag="JN749_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HAY7369170.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13678..13905
                     /gene="rpsR"
                     /locus_tag="JN749_002814"
     CDS             13678..13905
                     /gene="rpsR"
                     /locus_tag="JN749_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAY7369171.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            13947..14396
                     /gene="rplI"
                     /locus_tag="JN749_002815"
     CDS             13947..14396
                     /gene="rplI"
                     /locus_tag="JN749_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAY7369172.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14467..15024)
                     /locus_tag="JN749_002816"
     CDS             complement(14467..15024)
                     /locus_tag="JN749_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7369173.1"
                     /translation="MNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDVE
                     SFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQLR
                     TVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRSD
                     RMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3509 a         3662 c         4050 g         3806 t
ORIGIN      
        1 tgttaggtta cgttatccca ttatccagtg cggcaatcaa tgcggtgagt attccagact
       61 atttcgcctg gggcgggatt gcactggtga ttcagttact cgtttttgct ggtgtcagac
      121 tttatatgcc cgcattaagc gaaaaaatta ttaatcacaa taccgcagca ggaatgttta
      181 tgggaaccgc cgcgctggct ggcggtattt ttaacgcagc ttgtatgaca tggtaatgga
      241 cgatcatggc cagaaaacgc aaatcaagaa ataacagtaa aattggtcac ggagcgatca
      301 gtcgcattgg tagaccgaat aatccttttg aaccgcgtcg caatcgctac gcacaaaaat
      361 acttaacgtt ggcgctaatg ggcggtgccg cttttttcgt attgaaaggt tgtagcgata
      421 gtagcgatgt cgataacgac ggcgacggaa cgttttacgc gacagtgcag gattgtattg
      481 atgacggtaa taatgccgac atttgcgcgc gtggctggaa caacgccaaa gcggcatttt
      541 atgccgatgt tccaaagaat atgactcagc agaactgtca gtctaagtac gaaaattgct
      601 actacgacaa tgttgaacag agttggatcc cggtcgtttc tggatttttg ttaggccggg
      661 ttattcgtaa agatcgcgat gagccgttcg tctataacag cggcggatcc tcatttgctt
      721 cccgccccgt ctggcgcaac acttccggtg attactcctg gcgcttcggt tctggcaaaa
      781 aagagtctta ctcttcaggc ggctttacca ccagaaaagc gtcgaccgtt tcgcgcggcg
      841 gctatggtcg ttcttccagc gcccgtgggc attggggagg ctaatcatgc tgagacacaa
      901 cgttcctgtg cggcgggatc tggaccagat cgccgctaac aacggtttcg actttcatat
      961 catcgacaat gaaatctatt gggacgagag tcgggcttac cgttttactc tgcgccagat
     1021 tgaagagcag atcgaaaaac cgactgcgga actgcatcag atgtgccttg aggtggtgga
     1081 tcgcgcggtt aaagacgaag agatcctgac gcaactggcg atcccgccgt tgtactggga
     1141 tgtgatcgcc gaaagctggc gcgcacgcga tccttcgctg tatggccgga tggattttgc
     1201 ctggtgtggc aatgcgccgg tgaagctgct ggagtacaac gccgatacgc cgacctcgtt
     1261 gtatgagtcg gcttatttcc agtggttgtg gctggaggat gcccggcgca gcggcattat
     1321 tccgcgtgat gccgatcagt acaacgccat tcaggaacgc ctgatttcgc gctttagtga
     1381 gctttacagt cgggaaccgt tttatttttg ctgctgtcag gacaccgatg aagacaggag
     1441 taccgtgctg tacttgcagg actgcgccca gcaggcaggg caggagtcgc ggtttatcta
     1501 cattgaagat ctcggtttgg gcgtcggcgg cgtactgacc gatcttgatg ataatgtcat
     1561 ccagcgtgca tttaagctgt atccgctgga gtggatgatg cgtgacgata acggtccgct
     1621 gctgcgcaag cgtcgcgagc aatgggtgga gccgttatgg aaaagtatct tgagtaataa
     1681 agggctaatg ccgctgcttt ggcgcttctt ccctggtcat cctaaccttc ttgcatcctg
     1741 gtttgagggt gaaaaatcgc agattgccgc tggcgaaagc tatgtgcgta aaccgatcta
     1801 ctcgcgcgaa ggcggaaacg tcaccatttt cgacggtcag aataacgtcg ttgaccacgc
     1861 tgatggtgat tacgccgatg aaccgatgat ctaccaggcg tttcaacctc tgccgcgatt
     1921 tggcgatagc tacacactca tcggtagctg gattgtcgat gatgaagcgt gcggaatggg
     1981 gatccgtgaa gataacacac tgatcaccaa agacacctca cgtttcgttc cgcattacat
     2041 tgctggataa gaatgtttta gcaatctctt tctgtcatga atccatggca gtgaccatac
     2101 taatggtgac tgccattgat ggagggagac acagtgcact ggcaaactca caccattttt
     2161 aatcaaccta taccattaaa taacagcaac ttatacctgt ctgatggcgc gctttgcgaa
     2221 gcggtaacgc gtgaaggtgc tggctgggat agcaattttc tagccagtat tggtcagcag
     2281 ttaggaacgg ctgaatccct tgaactgggg cggctggcga atgtaaaccc gcctgaactg
     2341 ttgcgctacg atgcccaggg gcggcgtctg gacgatgttc gtttccatcc cgcatggcac
     2401 ctgctgatgc aggcgttatg taccaatcgg gtgcacaatc ttgcctggga agaagacgct
     2461 cgctccggcg catttgtggc gcgcgcggcg cgttttatgt tacatggcag gttgaggctg
     2521 ggtcgttatg tccaataacg atgacctttg ccgccacgcc attgttgtta cagatgttac
     2581 ccacgccgtt tcaggactgg accacgccgc tgctgagcga tcgctacgat tctcacttat
     2641 tgccaggtgg gcaaaaacgc ggtttgttga ttggcatggg aatgacggaa aagcagggcg
     2701 gttccgatgt catgagcaac accatccgtg cagagcgttt ggaagatggc tcttatcggc
     2761 tggtggggca taaatggttt ttctcggtgc cgcaaagcga tgcgcatctg gtgctggcgc
     2821 agaccgcggg cggtctgtcc tgcttttttg tgccgcgctt tttgcctgac gggcaacgca
     2881 acgcgattcg cctcgagcgg ctgaaagata agctgggtaa tcgctctaac gccagttgcg
     2941 aagtggagtt tcaggatgcc attggctggt tattggggca ggaaggggaa ggaattcgtc
     3001 tgatcctgaa aatggggggg atgacgcgtt ttgattgcgc cctgggtagc catgccatga
     3061 tgcgccgtgc attttcgctg gcgatttatc atgcacatca acgccatgtt tttggtaatc
     3121 cattgatcca acagcccctt atgcgtcatg tcttaagtcg catggcactt cagcttgaag
     3181 ggcaaacggc gttgctgttt cgtcttgcgc gagcgtggga ccggcgtgcc gatgccaaag
     3241 aagccctgtg ggcgcgttta tttacgcctg cggcaaaatt tgtgatctgc aaacgaggta
     3301 tgccgtttgt ggccgaagcg atggaggtgc tgggcggcat tggttattgc gaggagagcg
     3361 agctgccgcg gctttaccgg gagatgccgg taaacagtat ttgggaaggt tccggcaata
     3421 ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac gacttattgt
     3481 cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg gttcgtcgtt
     3541 tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact catcagctat
     3601 tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg gcgcaggcgt
     3661 ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag atccagaatg
     3721 atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact gatgcgacgc
     3781 tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg cgctcgcgcc
     3841 gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc gcctgtacgc
     3901 gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat gacgcacctt
     3961 gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct gaaatattgc
     4021 taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg gcgaattctg
     4081 cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt gaggcaagta
     4141 attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca ttccatcgcg
     4201 gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac gcatagggac
     4261 gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta caaaatggct
     4321 tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac gtaataatca
     4381 gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg agaaccccga
     4441 accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg gcgaatctct
     4501 tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc gctacaggca
     4561 cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt acgtttcctg
     4621 ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg aattgttacc
     4681 tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc aacattatga
     4741 actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc agacaaaaag
     4801 gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt tcttcatgct
     4861 tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca cggctttact
     4921 tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg tttgcgggtg
     4981 atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc gcggcggtta
     5041 aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt acgtgcggca
     5101 atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc gtcgatgggc
     5161 gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac cgccagcatg
     5221 atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct gatacctgaa
     5281 acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga gtgggaagcg
     5341 acaaaccacc tagaacaact tggggacaga cctctactgc tgtggcatgg cctcgacgat
     5401 gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga gacgggacgg
     5461 gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac gcctgaggcg
     5521 ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat gctgacccct
     5581 tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat cagcatatcg
     5641 atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact gctatcaacc
     5701 agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc tgtcagcata
     5761 tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat ccagtgcccg
     5821 gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact tttgttgtac
     5881 tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt tgccagcggt
     5941 agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag cagaaacgcg
     6001 gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga ggccgcttta
     6061 gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg cgtccagcgc
     6121 gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt gccgctttcg
     6181 ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg ctcaacgact
     6241 ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg atgtctttgt
     6301 gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt tgaaatagcc
     6361 aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa acgacagatt
     6421 acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg aaaccgcgcg
     6481 gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg ccaacctgcc
     6541 agataaacgg cttgaagcca tacttcagac gatctttttt catctcccac agcacgcgga
     6601 tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg ataaccactt
     6661 tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg tcggtaatac
     6721 cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg ttaccgtgct
     6781 tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg ccaggggttt
     6841 tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca gccgcttttt
     6901 gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca tgaatttcaa
     6961 tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct ttcggtacgc
     7021 cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg tcatcagcac
     7081 agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga tcgtgagtcg
     7141 ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg gtggtgcgta
     7201 ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc tgtttcatta
     7261 acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa acgttggtgc
     7321 caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca aatgtgccag
     7381 gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc gggaaagtgc
     7441 tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt taccttcatg
     7501 atagttcaat tcgaatcaat atgtgattgg ttttgattaa tcctgacact attttttcag
     7561 gaaggcaatg accatttttt gactttttgc cagggaagtt gttgttgatt ttgagtgtag
     7621 aaagatttaa tggaatgtat agttaaatta actaaatgaa tttaaataaa taattttttc
     7681 gttgtgtgaa tcccactcta tccgtgtgga attatttgcg ggtcgcgtca catttaatca
     7741 taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca ttccgggtgt
     7801 gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct acaacatctt
     7861 taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta ttgtgacctg
     7921 tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca cgattaaaac
     7981 cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca ccttcaaacc
     8041 ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg atacttacgc
     8101 gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag gttacgcggt
     8161 gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta ccggtattcg
     8221 taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg ccgttacgtt
     8281 gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc tggtttcgct
     8341 ctactggggc attacctcca acatgatgta caagccgact caggaagtga cggacggctg
     8401 cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata aagttgcgcc
     8461 gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct ggctgaatat
     8521 tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg gtgccattct
     8581 gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact ggacggtcta
     8641 tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc agggtgtgcg
     8701 catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc tgatccctgg
     8761 tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg ccgtggtctg
     8821 gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg tcgcctgcgg
     8881 ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg ccaccatcgg
     8941 cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc tggtgatggg
     9001 gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga gtgcctggat
     9061 ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct tctccatcgg
     9121 tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct tcgctggccg
     9181 cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg cttaataagg
     9241 agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag gcagttccat
     9301 gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc acacggtaaa
     9361 tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca tcatcgcttc
     9421 tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg gcgtgcgcaa
     9481 catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag cgcatttccc
     9541 gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg agaataaatc
     9601 catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg gcgttgacct
     9661 gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg atgcggttga
     9721 acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg gtcgcccgga
     9781 agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc tggagttcaa
     9841 ccacgaagat aacgacccgg tggatatcct catcaccatg gcggtggtcg atgccaacac
     9901 gcactaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag aaaatttcga
     9961 ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc gcaccaacgc
    10021 ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg ctggacaacc
    10081 agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc gacattatcg
    10141 aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat ctgaaagcgc
    10201 tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc aaaattcttt
    10261 ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt gcggatatca
    10321 acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg cagatcgaac
    10381 tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt attcagcagg
    10441 tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt gaagccgata
    10501 tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc ggtggcctgg
    10561 cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc gccggtcgca
    10621 gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt tccatcgccg
    10681 aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc atctatgaaa
    10741 aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa acgttgggct
    10801 tcgattttgt cgagatgtcg gtagatgaaa ctgacgagcg cctgtcgcgc ctcgactgga
    10861 gccgcgagca gcgtctggcg ctggtgaatg cgattgttga aaccggcgtg cgtgtgccgt
    10921 ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac gcggtgcggg
    10981 cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt ggcattcgcg
    11041 tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa acgcgtcgtc
    11101 gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcacag gtgacgctgg
    11161 cgatggagat catggattat ccgttgatga actccatcag caaggcgctg ggttacgcgc
    11221 actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg tcggcgtggg
    11281 acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg gtgcatgtga
    11341 aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg gtggatttcg
    11401 aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg attgagatgt
    11461 ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgcgcatg gccaaagcag
    11521 gcatggtgga ggcggcataa tgcaaaagct aaaacagcag gtgtttgaag ccaacatgga
    11581 tctgccgcgc tatgggctgg tgacctttac ttggggcaac gtcagcgcta tcgaccgcga
    11641 acgcgggctg gtggtgatca agcccagcgg cgttgcctac gaaaccatga aagcagacga
    11701 tatggtggtg gtcgatatga gcggcaaggt ggtggaaggg gagtatcgcc catcttccga
    11761 cactgcgacg catctcgaac tctaccgtcg ttacccgtcg cttggcggca ttgtccatac
    11821 ccactccact catgccaccg catgggcgca ggcggggctg gcgatcccgg cattaggcac
    11881 cacgcacgct gactacttct ttggtgatat cccgtgtacg cgtggattaa gcgaagaaga
    11941 ggtgcagggt gagtatgaac tgaacactgg caaagtgatt atcgaaacgc tgggcaacgc
    12001 cgagccgctg catacgccgg gaattgtggt gtatcagcac ggaccgttcg cctgggggaa
    12061 agatgcccac gacgcggtgc ataacgcggt agtgatggaa gaagtggcga aaatggcgtg
    12121 gattgcgcgc agtattaatc cacaactcaa tcatatcgac agctacctga tgaataaaca
    12181 cttcatgcgt aagcacggtc ctaacgctta ttacgggcag aagtaaaaca cgcgctgcgg
    12241 aaatttcctt cctcgggaga taactggtct aattccgcag ccgtttttca aaaaaagccc
    12301 cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg
    12361 ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaattac
    12421 gtggtagtac gttgcgcctt gcgcatctgt ttcttcattt aatgcctgac gtgcttcgct
    12481 ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat cgtccatatt
    12541 tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac
    12601 agccagaagg gctaaaactc gactgaacat agatacctcc tcgacggctg actttgtgtg
    12661 ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag ttgatggtga
    12721 ccatttctga tgcagttgtt caaaaaaaca ctgtgacgga atgtgatgaa cttcaaatca
    12781 gcggtttaga ggttatttgc gaaaggggag atttatttct gctccgccct tgagtttagc
    12841 ggggtatgtc agtactataa cgcgtcattt ttcagccgac ctttaacacg ttccttgcct
    12901 ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc aattcgatgc
    12961 gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt ccgggcatga
    13021 tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt ctggaagact
    13081 ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac tacgttctga
    13141 tgaacattga agctccgcag gaagtgatcg atgagctgga aactaccttc cgcttcaacg
    13201 atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa gcatctccga
    13261 tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg
    13321 atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt ctggtgttgt
    13381 ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc
    13441 agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ctttcaccgg caggcatggt
    13501 gtcaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac agtataacgg
    13561 tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag aacggactga
    13621 gcaaaatggt tttgcacgcc gagcagattg aattgataga ttctggagac tagccatatg
    13681 gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt tcaagagatc
    13741 gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa gattgtccca
    13801 agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc
    13861 gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt cacggtccat
    13921 taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa gtagcaaacc
    13981 tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac
    14041 cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa gcacgtcgcg
    14101 ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc gctgagaaaa
    14161 tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg
    14221 gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt gaagtggcta
    14281 agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgaacac gaagtgagct
    14341 tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg
    14401 ttattcaacg agacgtaaaa acgccgacca ttggtcggcg ttttgctttc tatttttcgt
    14461 caggcattaa tttcgcaagt agatccattc accattctct tgtgtccata agtcaggatg
    14521 gtttgctggc gttagatgca tacgatcact ccttatgtct cggtcctgcg gcactcgctc
    14581 gaaacctaaa cccgagtaga ttgcggctga atttgataag gaacgtagac caattttcaa
    14641 atcatggggt aaatcagaat acgcgtttag caatgcagca atgatgaatc cgagggcatt
    14701 agaaacgcct gtaacagtgc gaagttggtc gatataaaga tcgattgttg gtagttcaga
    14761 tgaccagcaa ttaggcattt gggcaggaag ggttacaacg tatcctatcg tttggccggt
    14821 atagtctgga gtactataac caggtcccaa aacaacctcg tgtattgtgt atttactgtt
    14881 gggttcgaag gattcgacat cttgcaataa atatttcagt tctgcggtta acctcgcacc
    14941 cgcgtaagct ttattgtatt tctgggcgtg aatattatct aatgtagctt catggttcca
    15001 tccaaaagaa ttatcaccat tcattgt
//
LOCUS       DADLTF010000098        14955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.098, whole genome shotgun sequence.
ACCESSION   DADLTF010000098 DADLTF010000000
VERSION     DADLTF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.098"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..347
                     /locus_tag="JN749_002817"
     CDS             <1..347
                     /locus_tag="JN749_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HAY7369174.1"
                     /translation="QVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPASL
                     LTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRKI
                     RSEARHRLEEKQ"
     gene            344..799
                     /locus_tag="JN749_002818"
     CDS             344..799
                     /locus_tag="JN749_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369175.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="JN749_002819"
     CDS             complement(877..1788)
                     /gene="rdgC"
                     /locus_tag="JN749_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HAY7369176.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            1913..2821
                     /gene="mak"
                     /locus_tag="JN749_002820"
     CDS             1913..2821
                     /gene="mak"
                     /locus_tag="JN749_002820"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HAY7369177.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="JN749_002821"
     CDS             complement(2964..4145)
                     /gene="araJ"
                     /locus_tag="JN749_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HAY7369178.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="JN749_002822"
     CDS             complement(4271..7414)
                     /gene="sbcC"
                     /locus_tag="JN749_002822"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HAY7369179.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="JN749_002823"
     CDS             complement(7411..8613)
                     /gene="sbcD"
                     /locus_tag="JN749_002823"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HAY7369180.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            8803..9492
                     /gene="phoB"
                     /locus_tag="JN749_002824"
     CDS             8803..9492
                     /gene="phoB"
                     /locus_tag="JN749_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HAY7369181.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            9550..10845
                     /gene="phoR"
                     /locus_tag="JN749_002825"
     CDS             9550..10845
                     /gene="phoR"
                     /locus_tag="JN749_002825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HAY7369182.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            11252..12571
                     /gene="brnQ"
                     /locus_tag="JN749_002826"
     CDS             11252..12571
                     /gene="brnQ"
                     /locus_tag="JN749_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HAY7369183.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            12647..14020
                     /gene="proY"
                     /locus_tag="JN749_002827"
     CDS             12647..14020
                     /gene="proY"
                     /locus_tag="JN749_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HAY7369184.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            14176..>14955
                     /locus_tag="JN749_002828"
     CDS             14176..>14955
                     /locus_tag="JN749_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HAY7369185.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWF"
BASE COUNT         3316 a         3803 c         4024 g         3812 t
ORIGIN      
        1 tccaggtatt ttttgcggaa ctggctacga taaaagataa atcagatgat gaatggtggc
       61 gtgcattgct gcaaagtcgc aatgatcatt tgcgccaaac ggcattacgc aacgcacata
      121 ccccggcgtc gttgttaacg acattgactg agcctcagga tcggtcgctg gctattaata
      181 atccacagct ggctgccgat gtgaaaacgg cgtggttaaa agaggatcca tcattactct
      241 tatttgtcga acaacccgat ctttcgcagt tacgtgattt agtgaaaacc ggggcaacgc
      301 ggaaaattcg cagtgaagcg cgtcaccggc ttgaggaaaa acaatgacac aacgtccatg
      361 gagcaagtta cagcgggaga tctatgactt actgacgcca acgatcaacc tgcaaattca
      421 ctgtacccgt tatcccatgc gcagccaaaa tggtggaagt accgatttac cgcgttattg
      481 gatcacgttg gataaaaacg tgatctggga ttacccgaaa gactttattg caggtaacgc
      541 tggagtgcgt aattttcacg gtgaaacttg ctggtatcct tacctgacgg atatttgttc
      601 aatttctgat ttattgcgtg aatatattga taccccaaaa gcggagctgt taacaaaaca
      661 atttaccagc gataaatggg ggttagtgaa tattctgcgt gctgctgaca ggcgaatagg
      721 gatgaggcga ttagatcagt tacggcgtaa aacgcataat attgctgcgc taaaaattat
      781 tgctcgccgt agcgaataat tatatgcctg gtgtggcttc gtacgccgga taagacgcgg
      841 caggcgtcgc atccggcatt aaaggaaaat cagcaattaa cgttgtgctt cgccacctaa
      901 tccttcaatc aggttttgaa ttaacgctgc cagttcacca gtcatcagga tgaaatcggc
      961 atcaaaacgc tgggcgaaat cttcacggtc gatatcttcg ttttgatcgt gcagctcgtc
     1021 gcagaactta agacgcttga gcgaaccatc gtcgcacatc acaaactgaa tgcgctgctg
     1081 ccagtcgagc gccagtttag tcaccacttt tccggcttca atgtgattgg tgatctcttc
     1141 gctggtcaga tcttgtttct tcgcgcggat cacgccgcca tcttccagca acgatttcag
     1201 ctcggcttca tcaagcagct ggaagccctg tgccgcacta ccggagcgaa cccattcggt
     1261 cagcgtcagt tcaatcgggt tttccatgct caatggtaca accggtaacg accccaggct
     1321 tttacgcagt aatgccagcg tatcttccgc ttttttggca ctggcgcagt ccaccataat
     1381 caaaccgtta accgtgtcga gccacatcat tgtctggcta aaacggctga aagcacgcgg
     1441 cagcagagag tgcagcactt cgtctttcag cgaatctttt tcggttttct tcagcttacg
     1501 cgcctgttcc gcttccagtt tggcgatttt cgcttccagc gcctgtttaa tcaccggaga
     1561 cgggaggatt ttttcttctt tgcgcgcgca gataacaatt tgaccattgg caacgtgcgt
     1621 taacgcatcg ctgtgcgatc ccatcggagg aacccagccc atcttcgcca tgtcctggct
     1681 gccgcatggg gtaaatgcca tcgaggctag ctgtttttcc atctcttctg cacgcagcga
     1741 aatctcgcgg ctaagacggt aaaccattaa atttttgaac cacagcatga taatttccac
     1801 ggccttgtcg ttaaatttag cgggcatgat aacgaattgt cggcggcctt gcattgccaa
     1861 tccggttgtc cgtctctacg ctattgatat tgaaaaaaat aaggagagta cagtgcgtat
     1921 aggtatcgat ttaggcggca ccaaaactga agtgattgca ctgggcgatg caggggagca
     1981 gttgtaccgc catcgtctgc ccacgccgcg tgatgattac cggcagacta ttgaaacgat
     2041 cgccacgttg gttgatatgg cggagcaggc gacagggcag cgcggaacgg taggtatggg
     2101 cattccaggt tcgatttcgc cctacaccgg tgtggtgaag aatgccaatt caacctggct
     2161 caacggtcag ccattcgata aagacttaag cgcgaggttg cagcgggaag tgcggctggc
     2221 aaatgatgcc aactgtctgg cggtttcaga agcagtagat ggcgcggcag cgggagcgca
     2281 gacggtattt gccgtgatta tcggcacggg atgcggcgcg ggcgtggcat tcaatgggcg
     2341 gacgcatatc ggcggcaatg gcacggcagg tgagtgggga cacaatccgc taccgtggat
     2401 ggacgaagac gaactgcgtt atcgcgagga agtcccttgt tattgcggta aacaaggttg
     2461 tattgaaacc tttatttcgg gcacgggatt cgcgacggat tatcgtcgtt tgagcggaca
     2521 tgcgctgaaa ggcagtgaaa ttatccgcct ggttgaagaa agcgatccgg tagcggaact
     2581 ggcattgcgt cgctacgagc tgcggctggc aaaatcgctg gcacatgtcg tgaatattct
     2641 cgatccggat gtgattgtcc tggggggcgg gatgagcaat gtagaccgtt tatatcaaac
     2701 ggttgggcag ttgattaaac aatttgtctt cggcggcgaa tgtgaaacgc cggtgagtaa
     2761 ggcgaagcac ggtgattcca gcggcgtacg cggcgctgcg tggttatggc cacaagagta
     2821 aaaaacgtag gcaattggcg catcatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     2881 tacaaaatgt gccagaaccg cgtagggcgg ataaggcgtt cacgccgcat ccggcaataa
     2941 gcaacgcgca ccgtgacagt aatctacccc agtggtttcg ccagcaccgg actatccgcc
     3001 gcttgctggc gcttatagcg accatacagc agcaacgatg acatcgcagc aaacgaaaac
     3061 agggcggcag gcagtgccac gtaattatat gccagcccca gcgtcagcat catacctccg
     3121 caatatgcgc cgacggcgct accgaggtta aacgctattt gcccacctgc ggcacctaat
     3181 aactctccgc ctttggcatt ttgcagtaac aatatttgca gaggtgctga aagggcaaat
     3241 aatcccgcgc aacaaataaa agcaaaaata agcgacgttg ttttcatgcc gccgcagaaa
     3301 aagagcatca gcagtgccag tacaatgata aagtcagtca ctgctgcaat gcgcagtggt
     3361 gaataacgtc ctgaaatcct gccacttagc atatttccca gcaccatccc tagcccaact
     3421 aacatcataa taaaggtcat cgccgtttcc gaaaaaccgg aaataaacat catgtatggc
     3481 tttacgtagc tgaaccaggc aaacacacct gcgttgccaa acatcgtggc ggcgaaaatt
     3541 aaccacgggg ccgggctgcg caaaaagtga aattgttcgc gcagatttcc tttcgcctcg
     3601 tcgcgaatat ctggcaccca aaaatagacc gatgccatca ccgcaatatt aaaaacagcg
     3661 atcaataaaa aggtgtaacg ccagcattcc tgacttaaat acgttcccag cggaatgccc
     3721 agcaaattgg cgactgtcat cccggaaacc atccccgcca cggcggcggt gacttttccg
     3781 ggtttgataa tttttgataa cacgatcgct ccgacgccaa aaaatgcgcc atgcggaaag
     3841 ccggatacca gccgaccaat ggcgagcatc aggtaagacg aagagagcgt gaacatggcg
     3901 ttgccaatga cgcacaacgc caccagaaac aacaagatat gtttgagtga gtagcggctg
     3961 gaaaagagtg cgatgattgg cgcaccgacc accaccacca gtgcataata cgagatcata
     4021 tgcccggcag caggaatcga aattcctacg ttatgcgcca gctccgtgag cacgctcata
     4081 atgccaaatt cggctatccc caaaccaaac gtgcccagag ccaaagataa aatgactttt
     4141 ttcataccac catcacaacc accagaacgg ctaccaatca ccgtagccaa taaagtgata
     4201 gcttgcagga taacgaccag caaggccaag agataattcg cccctctgta ttcattatcc
     4261 tgctgaatag ttatttcact gcaaacgtac tttccagttt gctgtagccc aggccgttga
     4321 tctttttcac tttgatctgc accggaatac gctctttcat cgcttctacg tggctaatca
     4381 caccgatggt tttgccactg gcgttcaggg catccagcgc atcaagggcg gtatccagcg
     4441 tttcgctatc cagcgtgcca aaaccttcat caaggaacag cgagtcaata cgtgttttat
     4501 ggctgaccag atccgaaagc gccagcgcca gcgccagact aacgaggaaa ctttcgccgc
     4561 cggaaagggt acgggtatcg cgtaccgcat ctgcctgcca ggtatcaaca acctcgactt
     4621 ccagcgcctc gctggctttg cgctgtaaca gatagcgccc gtgcagccgg gtaagttgct
     4681 gattagcgag atggactaaa ttatccagcg tcagcccctg ggcaaacttg cggaatttat
     4741 cgccctcttt ggaacctatt agcgaattca gatatcccca gtcctcaacc tgctgcgtca
     4801 tttgagcaat ttgctgcatt aaggtttgtt gttgctgacg gttatctgca tcctgcttca
     4861 gctgctggcg aatctcgcct tgactcgtgg tgttttcacg caacttttgg tgagtttgcg
     4921 ctaactcttg ctgaatctgc tccaccgtca cagtgagagc caacccgtca ggtcggtgtt
     4981 gttgatgctg tgccagcgtt tctgctgtct gagtgaccag cgtttgcgcc tgacggcgct
     5041 ggttttccag attctgcttg agctgttcca gctgcgttag tgtttgttca tccattagcg
     5101 ccgcaaggaa cgcctgctga tcgtcaaaga cgctggcctg tagcgtggtg tcaaactgcg
     5161 cttgggcttt ttgcagactt tgcgccgcca gaacatcctg ttgctgtaac gtctgctgct
     5221 ggctgtgtaa tgcgagacat tgttcatgaa cctgccgcca gttatccagc gccacagttt
     5281 cttcgctgtg cgggagatca tcactttgcg gcaacgtttc cagaatcggc gtcagctgct
     5341 gaatacggtt ttgcagcgcg gttaattcgt tctggcgttg ctgccagctc tgcgcttctt
     5401 gctgacgtgt cgccaaccag ctctcttctt catcttcctg tggcaatgtc agtgcataac
     5461 ccgccaatgc cgttaagagc tgctgctgac gttgttcaat ttgctgttga tactggataa
     5521 tttgctgatt atgcgcggca atctgccctt gtaattcatg tcgttggctg agtaaccgca
     5581 gctggcgttc gtgctcatct tgtgcatcca gccacggttg aatatcgtcc tgtggctgca
     5641 aggtgatatt gaggctggcc gtgacggctt gccattgttg agtaagtgct tgctcatctt
     5701 gtcggaggct ttgcgcttcg ttttcatcgc gctgaagctg cttcgttaat gcatccagtt
     5761 gcccgcgcag cgccgcacct tcttcgccga gctttttaac ttcgttttcc agcgccagta
     5821 atcgagactg attaacacca ggctccagcg cctgatacgc ctcgaccgcc gggtggctgg
     5881 tggaaccaca aagtggacaa ggctgacccg cctgtaactg tgcacgctgg gcttccagcg
     5941 ttttgatgcg cgcttcctgc tcgcaaatgg ttttcacatc ggcaagttgc tgcgtctttt
     6001 ctttataacg ctggcgcatt tcgttaagtg cgacattacg ttgcgtctgt tccagagtga
     6061 cattctggat agtgaccatt aactgcgcca gacgtttttg ttggggaaca atctgcccat
     6121 gcagcgcgac caggcgctga cgcagtgggc gttgctcggc atgttgcgcc agggcggtag
     6181 caacttcatc ggcggttaac gtcaacgtga tcgccgcaag cgcattaagt ttttgctcag
     6241 catgggttaa ctgttgctgc cattgccgca gatgctcgcg atcgctggtt tgttgggaga
     6301 actgcgcacg ccaacccgcc agttcgttgt tccactgacg gaagcggtcg tgttcctgta
     6361 accaggtatt caggctttgt tgctgctgct gtaattctgc tgactgcttc gccgcgtggt
     6421 ggcgaatgct cgcgcgaagc gccattgtgc tctgtaagcg agtatttact tcttcaatct
     6481 gctggcgaat atgcgccagc gccgcgctgt gttctgcgat gcgttcccag tgtggacgaa
     6541 gatttcgtgc cggttgtgcc agactaagcg ccgccagttg aggttgcgct ttttcttctt
     6601 cggctaacgc ctgttgcaag gcctgctgac ggcggctggc ttcttgctgc aattcgtcct
     6661 gacgcgttaa ccagtttagc gattgttgtt cttgctgctg cgcggtaatt aactgttttt
     6721 cttcgtcagt aagtacctgc aaactcgctg tcagcgattg cacttgttcc ggcgtgagca
     6781 acgtgacgcc gctggcctgc gcttgcagct tctccagctc agtacgggcc gatttgtgct
     6841 gctcaaaaac catcgccgag atttgcccgt agatttcagt gccggttaac tcctcgagca
     6901 attccgcgcg ttctttgggt ttggcattca ggaaggcagc aaattgcccc tgcgaaagca
     6961 gcatcgaacg ggtgaagcgc ccgtaatcca gcccggttaa cgtcgctgtc agttccagct
     7021 tatctttcac tttgtcggcg agaattttgc cgtcggcgca gcgcgccagc tctacgcgag
     7081 gcacctgcaa attaccgtcc ggctggttac gcgcccgatt ctggctccag aacgcgcgat
     7141 acgcttcacc tttcacttca aactccacct ccgccagaca ttcagcggta tcgcgggtca
     7201 tgagatcatt ttgcgattgt gaaacgttag agagacgcgg agtttcgtga tacagcgcca
     7261 gacaaatggc gtccagcagg gtggttttcc ccgcaccggt tgggccggta atggcaaaca
     7321 gcccgttgct ggcgaacggc tcgcgggtga aatcaatctt ccattcgcct tttaatgagt
     7381 tcaggttttt caggcgcagg ctgagaattt tcatgcttcg tgttctccgg cgagggtatg
     7441 caacgtcgtg gtgaaaagat gctgcagacg ttgctgctgc gattcatcca gttcttccag
     7501 tgccagacgg cgattgaaca cctcttcgac gctgagttcg ctgagggttt cacgctgttg
     7561 gctggctaac acacgctcgc gctgttcacg actccgacgt accagcaata cttcgacagg
     7621 caatgattcg gttaatgcct ggattttgcg ctgaatatca tgcagatact catcagtagt
     7681 gatttcgata tccagccaga caggtggctc ctgcgataca tcgcgccact gttccagctg
     7741 tgcggtaatc gacgccagat cgcctttcag caccgccatg ggttgagtta ccggtacgtt
     7801 caggttttcc acgctctcta atttgccgtt tgaaaatgtc accagatgga catatttact
     7861 cttaccgcat tcatcaaaac tcagtggaat gggggaaccg caatagcgaa catgttccat
     7921 gccgccaata atctgtgcgc ggtgaatatg cccgagcgcg atgtagtcgg ctggtggaaa
     7981 gttttgtgcc ggaaacgcgt ccagcgtgcc aatataaatg tcacgcacgg cgtcactttt
     8041 actggcaccc acggtcgtta aatgtcccgt ggcgatgatg ggcagaggct gatcgccgcg
     8101 cagtttgcag gcatcggcat agtgttgttg gtaataatcg gtaatcgctg ccagtaaatg
     8161 ctgctgtttt tcaataccgt taagccccgc ctggctggaa ataatgtcac gcgggcgtaa
     8221 aaacggaatg gggcacagca ctgcgcctgg cgtcccgtcg cgacgaggca agatttgcgg
     8281 cgcatgtccg gcgctggcga ccacggttgt attgaggaac gccatgatat cgcgcgattc
     8341 attcagcgtg gcgaccgagt catggtttcc tgccagtacc accagatgac agccagtttg
     8401 ctgtaaattg acaacaaaac ggttgtataa cgtgcgggcg taactgggcg gcgagccggt
     8461 atcgaaaaca tcaccagcaa caataatcgc atccacctga tgggtttgtg ctgtctccag
     8521 cagccagtca agaaaagcct gatgttcagc ttcgcggctt ttactgtaga agttctggcc
     8581 gagatgccag tctgaggtgt gaaggatgcg cataacggtt ccctggcgaa aaagcatggt
     8641 cgcgattata cccaaacaga tgtgccattt gcttttttct gcgccacgga aatcaataac
     8701 ctgaagatat gtgcgacgag cttttcataa atctgtcata aatctgacgc ataatgacgt
     8761 cgcattaatg atcgcaacct atttattaca acagggcaaa tcatggcgag acgtattctg
     8821 gtcgtagaag atgaagctcc aattcgcgaa atggtctgct tcgtgctcga acaaaatggc
     8881 tttcagccgg tcgaagcgga agattatgac agtgctgtga atcaactgaa tgaaccctgg
     8941 ccggatttaa ttctccttga ctggatgtta cttggcggct ccggtatcca gttcatcaaa
     9001 cacctcaagc gcgagtcgat gacccgggat attccagtgg tgatgttgac cgccagaggg
     9061 gaagaagaag atcgcgtgcg cggccttgaa accggcgcgg atgactatat caccaagcca
     9121 ttttcgccga aggagctggt ggcgcgaatc aaagcggtaa tgcgccgtat ttcgccaatg
     9181 gcggtggaag aggtgattga gatgcaggga ttaagtctcg acccgacatc tcaccgagtg
     9241 atggcgggcg aagagccgct ggagatgggg ccgacagaat ttaaactgct gcactttttt
     9301 atgacgcatc ctgagcgcgt gtacagccgc gagcagctgt taaaccacgt ctggggaact
     9361 aacgtgtatg tggaagaccg cacggtcgat gtccacattc gtcgcctgcg taaagcactg
     9421 gagcccggcg ggcatgaccg catggtgcag accgtgcgcg gtacaggata tcgtttttca
     9481 acccgctttt aacgccttgc tcatcggacg cagagcaggg cttatgattt cttaactgga
     9541 gtatcttacg tgctggaacg gctgtcgtgg aaaaggctgg tgctggagct gctactttgc
     9601 tgcctcccgg ctttcatcct gggtgcattt tttggttacc tgccctggtt tttgctggca
     9661 tcggtaacag gactgcttat ctggcatttc tggaatttat tgcgcctttc atggtggctg
     9721 tgggtggatc gcagtatgac cccgccaccg gggcgtggta gctgggaacc gctactatac
     9781 ggcttacacc agatgcagct gcgaaataaa aaacgccgcc gtgaactggg caatctgatt
     9841 aaacgctttc gtagcggcgc ggagtcgctg cccgacgcgg tggtgctgac cacggaagag
     9901 ggcggtattt tctggtgtaa cggtctggcg caacaaattc ttggtttgcg ctggccggaa
     9961 gataacgggc agaacatcct taacctactg cgttacccgg agtttacgca atatctgaaa
    10021 acgcgtgatt tttctcgccc gctcaatctg gtgctcaaca ccgggcggca tctggaaatt
    10081 cgcgtcatgc cttataccca caaacagttg ttgatggtgg cgcgtgatgt cacgcaaatg
    10141 catcaactgg aaggggcgcg gcgtaacttt tttgccaacg tgagccatga gttacgtacg
    10201 ccattgaccg tgttacaggg ttacctggag atgatggatg agcagccgct ggaaggcgcg
    10261 gtacgcgaaa aagcgttgca caccatgcgc gagcagaccc agcggatgga agggctggtg
    10321 aagcagttgc taacgctgtc gaaaattgaa gcctcgccga cgcaattgct caatgaaaag
    10381 gttgatgtgc cgatgatgct gcgcgttgtt gagcgcgagg ctcagactct gagtcagaaa
    10441 aaacagacat ttacctttga gatagataac ggcctcaagg tgtctggcaa cgaagatcag
    10501 ctacgcagtg cgatttcgaa cctggtctat aacgccgtga atcatacgcc ggaaggcacg
    10561 catatcaccg tacgctggca gcgagtgccg cacggtgccg aatttagcgt tgaagataac
    10621 ggaccgggca ttgcaccgga gcatattccg cgcctgaccg agcgttttta tcgcgttgat
    10681 aaagcgcgtt cccggcaaac cggcggtagc ggattagggt tagcgatcgt gaaacatgcg
    10741 gtgaatcatc acgaaagtcg cctgaatatt gagagtacag taggaaaagg aacacgtttc
    10801 agttttgtta tcccggaacg tttaattgcc aaaaacagcg attaatccgc ctttgtcatc
    10861 ttttattgcc ataagccagc cgatgctggc ttattttctt tgcagtcaaa atacgggcgt
    10921 tagattttac aacggttggt gattttttgt tcgcatgatt agccatgtct ttttcacgga
    10981 aatagtgttt tatactggtt ggtgatttct tatcgctata tacctctggt ttttagatcc
    11041 ctccttgctt taaaacgtta taagcgttta aattgcgctt caggtgctgt catactgact
    11101 gcattaacgc ggtaaatcga aaaactattc ttcgccgcgc ctggttggga gtatttcccg
    11161 ctaaaattgt ttaaatatac cgctgtatca tccccaggga ttggcacaaa aatttaacgt
    11221 tacaacacca catccacagg cagtatgatt tatgacccat caattaagat cgcgcgatat
    11281 catcgctctg ggctttatga catttgcgtt gttcgtcggc gcaggtaaca ttattttccc
    11341 tccaatggtc ggcttacagg caggcgaaca cgtctggact gcggcattcg gcttcctcat
    11401 tactgccgtt ggcctgccgg tattaacggt agtggcgctg gcaaaagttg gcggcggtgt
    11461 tgacagcctc agcacgccaa tcggtaaagt cgctggcgta ctgctggcaa cggtttgtta
    11521 cctggcggtg gggccgcttt tcgctacgcc gcgtacagct accgtttcct ttgaagtggg
    11581 gattgcgccg ctgacgggtg attccgcgct gccgctgttt atctacagcc tggtctattt
    11641 cgctatcgtt attctggttt cgctctatcc gggcaagctg ctggataccg tgggcaactt
    11701 ccttgcgccg ctgaaaatta tcgcgctggt catcctgtct gttgtcgcga ttgtctggcc
    11761 ggcgggttct atcagcacgg cgactgaggc ttatcaaaac gctgcatttt ctaacggctt
    11821 cgttaacggc tatctgacca tggatacgct gggcgcaatg gtgtttggta tcgttattgt
    11881 taacgcggcg cgttctcgtg gcgttaccga agcgcgtctg ctgacccgtt ataccgtctg
    11941 ggctggcctg atggcgggtg ttggtctgac tttgctgtac ctggcgctgt tctgtctggg
    12001 ttcagacagc gcgtcgctgg tcgatcagtc tgcaaacggc gctgctattc tgcatgctta
    12061 cgttcagcac acctttggcg gcggcggtag cttcctgctg gcggcgttaa tcttcatcgc
    12121 ctgtctggtc acggcggttg gcctgacctg tgcttgtgca gaattctttg cccagtacgt
    12181 accgctctct tatcgtacgc tggtgtttat cctcggcggc ttctcgatgg tggtgtctaa
    12241 cctcggctta agccagctga tccagatctc cgtaccggtg ctgaccgcta tttatccgcc
    12301 gtgtatcgca ctggttgtat taagttttac acgctcatgg tggcataatt cgtcccgcgt
    12361 gattgctccg ccgatgttta tcagcctgct ttttggtatt ctcgacggga tcaaagcatc
    12421 tgcattcagc gatatcttac cgtcctgggc gcagcgttta ccgctggccg aacaaggtct
    12481 ggcgtggtta atgccaacag tggtgatggt ggttctggcc attatctggg atcgcgcggc
    12541 aggtcgtcag gtgacctcca gcgctcacta aatcactgaa catttgtttt aaccacgggg
    12601 ctgcgatgcc ccgtggtttt ttattgtgtt gatgggttag gaattgatgg aaagtaagaa
    12661 taagctaaag cgtgggctaa gtacccgcca catacgcttt atggcactgg gttcagcaat
    12721 tggcaccggg ctgttttacg gttcggcaga cgccatcaaa atggccggtc cgagcgtgtt
    12781 gttggcctat attatcggtg gtatcgcggc gtatatcatt atgcgtgcgc tgggggaaat
    12841 gtcggtacat aacccggccg ccagctcttt ctcgcgttat gcgcaggaaa accttggccc
    12901 gctggcaggt tacattaccg gatggaccta ctgctttgaa atccttattg tcgccatcgc
    12961 cgatgtgacc gcttttggta tctatatggg tgtcttgttc ccgacggtgc cgcactggat
    13021 ttgggtactg agcgtggtgc tgatcatttg cgccgtaaac ctgatgagcg tgaaggtctt
    13081 cggtgagctg gaattctggt tctcgttctt taaagtcgcc accatcatca tcatgattgt
    13141 cgccggtttc ggcatcatca tctgggggat tggcaacggc gggcaaccga ccggtattca
    13201 taacctgtgg agcaacggcg gcttcttcag taacggctgg cttggcatgg taatgtcgtt
    13261 gcaaatggtg atgtttgctt acggtgggat cgaaattatc gggattaccg ccggtgaagc
    13321 gaaagatcct gagaaatcga ttccgcgtgc gattaactcc gtgccgatgc gtattctggt
    13381 gttctacgtc ggtacgctgt tcgtcattat gtctatctac ccgtggaatc aggttggcac
    13441 tgccggtagc ccgttcgtgc tgacgttcca gcatatgggc attacctttg ccgccagcat
    13501 tcttaacttt gttgtgctga ccgcttcgct gtcggcaatt aacagtgacg tatttggcgt
    13561 aggccgtatg ctccacggta tggcagagca gggcagcgca ccgaaaattt tcagcaaaac
    13621 gtcgcgtcgc ggtattccgt gggttacggt gctggtgatg actaccgcgc tgctgtttgc
    13681 ggtgtatctg aactacatca tgccggaaaa cgtcttcctg gtgatcgctt cgctggcaac
    13741 cttcgccacg gtatgggtgt ggattatgat cctgctgtcg caaatcgcct tccgtcgccg
    13801 tttgccgcca gaagaagtta aggcgctgaa atttaaagtg ccgggtgggg tagcaacgac
    13861 catcggcggg ctgattttcc tgctctttat tatcgggttg attggttatc acccggatac
    13921 gcgtatctcg ctgtatgtcg gtttcgcgtg gattgttgtg ctgttgattg gctggatgtt
    13981 taaacgccgc cacgatcgtc agctggctga aaaccaataa tacttgcgtt ctgtaagccg
    14041 gttcaggagg aaatcctgat ccggctattc cgaaagttat ccgcccccgt cctcctcccc
    14101 caaatatcct tcagatgatg agtgatcctg cattaggcta tggcaaggtg atcagatttt
    14161 catcacaggg gaattatgat gttaaatgca tggcacctgc cggtgccccc atttgttaaa
    14221 caaagcaaag atcaactgct cattacactg tggctgacgg gcgaagaccc accgcagcgc
    14281 attatgctgc gtacagaaca cgataacgaa gaaatgtcag taccaatgca taagcagcgc
    14341 agtcagccgc agccaggcgt caccgcatgg cgtgcggcga ttgatctctc cagcggacaa
    14401 ccccggcggc gttacagttt caaactgctg tggcacgatc gccagcgttg gtttacaccg
    14461 cagggcttca gccgaatgcc gccggcacga ctggagcagt ttgccgtcga tgtaccggat
    14521 atcggcccac aatgggctgc ggatcagatt ttttatcaga tcttccctga tcgttttgcg
    14581 cgtagtcttc ctcgtgaagc tgaacaggat catgtctatt accatcatgc agccggacaa
    14641 gagatcatct tgcgtgactg ggatgaaccg gtcacggcgc aggcgggcgg atcaacgttc
    14701 tatggcggcg atctggacgg gataagcgaa aaactgccgt atctgaaaaa gcttggcgtg
    14761 acagcgctgt atctcaatcc ggtgtttaaa gctcccagcg tacataaata cgataccgag
    14821 gattatcgcc atgtcgatcc gcagtttggc ggtgatgggg cgttgctgcg tttgcgacac
    14881 aatacgcagc agctgggaat gcggctggtg ctggacggcg tgtttaacca cagtggcgat
    14941 tcccatgcct ggttt
//
LOCUS       DADLTF010000099        14928 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.099, whole genome shotgun sequence.
ACCESSION   DADLTF010000099 DADLTF010000000
VERSION     DADLTF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14928
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.099"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            84..365
                     /locus_tag="JN749_002829"
     CDS             84..365
                     /locus_tag="JN749_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369186.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVIAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHGKKPERLRR"
     gene            complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="JN749_002830"
     CDS             complement(1162..2622)
                     /gene="phoQ"
                     /locus_tag="JN749_002830"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HAY7369187.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="JN749_002831"
     CDS             complement(2622..3293)
                     /gene="phoP"
                     /locus_tag="JN749_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HAY7369188.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3462..4832)
                     /gene="purB"
                     /locus_tag="JN749_002832"
     CDS             complement(3462..4832)
                     /gene="purB"
                     /locus_tag="JN749_002832"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAY7369189.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="JN749_002833"
     CDS             complement(4836..5477)
                     /gene="hflD"
                     /locus_tag="JN749_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HAY7369190.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="JN749_002834"
     CDS             complement(5513..6619)
                     /gene="mnmA"
                     /locus_tag="JN749_002834"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAY7369191.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGHEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="JN749_002835"
     CDS             complement(6673..7134)
                     /gene="nudJ"
                     /locus_tag="JN749_002835"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HAY7369192.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="JN749_002836"
     CDS             complement(7144..7797)
                     /gene="rluE"
                     /locus_tag="JN749_002836"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HAY7369193.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7969..9219
                     /gene="icd"
                     /locus_tag="JN749_002837"
     CDS             7969..9219
                     /gene="icd"
                     /locus_tag="JN749_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HAY7369194.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9322..9645)
                     /gene="iraM"
                     /locus_tag="JN749_002838"
     CDS             complement(9322..9645)
                     /gene="iraM"
                     /locus_tag="JN749_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7369195.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
     gene            complement(10178..10288)
                     /locus_tag="JN749_002839"
     CDS             complement(10178..10288)
                     /locus_tag="JN749_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369196.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
     gene            complement(10341..10745)
                     /locus_tag="JN749_002840"
     CDS             complement(10341..10745)
                     /locus_tag="JN749_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HAY7369197.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(10966..11697)
                     /locus_tag="JN749_002841"
     CDS             complement(10966..11697)
                     /locus_tag="JN749_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7369198.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(11902..13113)
                     /gene="bluF"
                     /locus_tag="JN749_002842"
     CDS             complement(11902..13113)
                     /gene="bluF"
                     /locus_tag="JN749_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HAY7369199.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            13427..13663
                     /locus_tag="JN749_002843"
     CDS             13427..13663
                     /locus_tag="JN749_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HAY7369200.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            13706..13978
                     /locus_tag="JN749_002844"
     CDS             13706..13978
                     /locus_tag="JN749_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HAY7369201.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            14007..14273
                     /gene="ariR"
                     /locus_tag="JN749_002845"
     CDS             14007..14273
                     /gene="ariR"
                     /locus_tag="JN749_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HAY7369202.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            14386..14634
                     /locus_tag="JN749_002846"
     CDS             14386..14634
                     /locus_tag="JN749_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369203.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         4006 a         3400 c         3478 g         4044 t
ORIGIN      
        1 gcatgaggat tttgcttttg atataagcga cagccaccgc cagaaactgg aagaatttag
       61 cggggtgtaa tgaggtcagc agcatgacag aagccgaaat aacgggatta atccgccgcg
      121 ttgccggaat cagccagcag gctgacgaac aggccacgca gccggacagc gtgatagccg
      181 aaaactatgc gcgtgttgtt gctgaggtga tgcgccgtga tggtatagaa cttaatggcg
      241 tggatatgcg caacatacga accagagttc ttgagttgct ggcctaccgt cgccgcgtgg
      301 agatgtatcg ggagaaggag aaaatcacgt accacgggaa gaagccggag cggttacggc
      361 ggtaactcac tgagattaaa gcaaagcgca aaattgcgct ggctacatcc ctcacatctg
      421 aggactgtta accagcaggc cggaatatat tcagaaacta cgaaattttc gcagtttaat
      481 tactcgtcag cttaatcgct aacccgctct gatattttcg gaaacctcaa tttgaggaag
      541 tcggcgaggt aactgtttga tattcccgat agcgcaaaaa tgcgcggtcg ggcagaatga
      601 ccaccacctc aatcgtgacg gagagtgaca accagtgaca caccgtgaca aaaagacggt
      661 ttaaccgttc attaaaaatc gacgtgattt tgtgctttcc ggatgacgtg gccaacgtca
      721 ttttttagcg aaaaattctg attaaggtca ctggctgaac ggtcataatg accacgcaga
      781 ccatcgaggc aaccatagcg atcatcgtta cggttggagc ttttccgcca ttggtggaaa
      841 aggtgcacat ctggatcatc attacggtga tcaagggtat gtaaaaccca ccagcctgat
      901 taaaggttaa ccggaaaaaa aatccggtag ccccgttatg gggatcccca tatcgacatt
      961 aacgcccctc atgaattggt gcgcttcccc ctggaaagat tacccgcctg tatatttctt
     1021 gtgtctattt gttccacgtt gtttcataca gtgcaccgaa cggtgtagtt aatggtgtag
     1081 tcaatttgcg atttttggca ctttttcaat gaaaattgtt taatcgaatc aatgacttga
     1141 tgtagtggta aaaggacata tttattcatc tttcggcgca gaatgctggc gaccaaaaat
     1201 cacctccatc cgcgcaccgc ccagcatgct ctctccggcg acgattttac cctcatattg
     1261 ctcggtgatt tcgcgggcta ccgccagccc tacaccttgc ccagggcgta aagtatcaac
     1321 ccgttgacca cggtcgaaaa tgacctctcg cttgcttaat ggaataccgg ggccatcatc
     1381 ctcgaccaca atatagagat gctcgtcggt ttgccttgca gaaatttcga caaactcgag
     1441 gcaatattta caggcattat ccagcacgtt gcccatcacc tcgacaaaat cgttctgctc
     1501 accgacaaag ctgatctctg gcgaaatatc gagagagata ttgacccctt tgcgttgata
     1561 cactttgttc agcgctgagg tgagattgtc cagcagtggg gcgaccggat gcagctcgcg
     1621 gctgagcaat gtcccgccgc gcatactggc acgatgcagg tagtagccaa tttgctgtga
     1681 aatgcggctg atttgctcca gcattaccgg ctcagcatca ctgacgctca tcttttcact
     1741 acgcagagaa cgcagcgtac tttgcagcac cgccagtggc gttttcagac tatgggtcag
     1801 gtcggtgagc gtcgtacggt atttgtcgta acgttcgcgt tcacttttta acaatcggtt
     1861 caggtttcgt accagactgg tcagttctcg cgttgtggct ggattgagca attcgcggtt
     1921 atgttcttcc agttcgcgga cttcttttgc cagggcttcg atggggcgta aactccacca
     1981 ggcggcgacc cacagcagcg ggatcactaa cagcagattg gctgagagta catagataaa
     2041 ccagctccag accatatagg aactttttag ctccaccgga atggtatcca ccaccacaat
     2101 ggttaatttt ggcatccgcg atgttgccgg gtagacgttt accgccaccg agtgggtcat
     2161 ctccgcgtcg tcatcatctt cccgcacttc ctgcaactgt tgctgtatcg aatgatctcc
     2221 actcagcaag aggctggtat cgttaacatc cgcttcaatt tcatgaaaac cattcgattt
     2281 cagccagtca ggctggatca ttttcatcag ccagggcacg tcacgttgcg cccataaaag
     2341 ctgcccgttc tcatcataaa ttagcgtcat ggtggggctt tgcttgtcga tattttcggg
     2401 taactcgaca tgcaacttat tgttttccca ctgcgcaagg gtatagaaca gattgctctc
     2461 gccacgtaac agccgaaacg tagttttatc gaaactgacg ctataaccga tcagcgcgac
     2521 cattccgtag gcaagcgaaa gcaccagtac taccgctgcc gttgccaaca gaaaacgtac
     2581 ccgcagcgag agcgggaaaa aaagacgcag taattttttc atcagcgcaa ttcgaacagg
     2641 tagccctggc cgcgaacggt ggtaatgact tcttgcggat attgtgcctg aatttttttg
     2701 cgcagacgtc ccatcagtac atcaatggta tggctttccc gcagctccgc atccggatag
     2761 agttgtagca ttaacgaatc tttgctgacc actttgccat tattgcgtat caacgtttcc
     2821 ataatggtgt attcgaacgc ggtcagtttg atcacttcgt cattaataga taattcacgg
     2881 cgagagagat caacctgaaa cggggggagc gaaatgacct gtgaagccag accgctatta
     2941 cgccgcatta atgcctgcat tcgcgccatc acctcttcaa tatgaaacgg tttagtcacg
     3001 taatcatcag caccggcact taatacttcg actttgtcct gccagctttc acgggcggtt
     3061 aataccagaa tcggcagtga aacatcgttg ctacgccagc ggcgaatcag tgacagaccg
     3121 tcctcgtcag gcaatccgag atcgacaatc gcaatatccg gtaaatgttc attgagataa
     3181 taatcggctt ctttggcatc ttctgcgtca tcgacctgat gaccagcatc ctgaatctga
     3241 actttaaggt ggtgacgtaa caacgcattg tcttcaacaa ccagtacgcg catttttatt
     3301 tctccctgtc ttaattatta aaatagtgta acgcgattat gtgtttatgg gggtaaacat
     3361 taaataaacc agcggggagg ggaggtaaag tgaaaaaaga aaaagtggat aatcttaata
     3421 agcaggccgg acagcatcgc catccggcac tgatttgagg cttatttcag ctcatcaacc
     3481 atcgtgatag ctcgaccaat atagttagcc ggcgtcatcg ctttcaggcg ggctttctct
     3541 tcttctggca acgccagacc atcgataaac tgcttcatgc cttcggcgtc aacgcgctta
     3601 ccgcgagtaa gctctttcag cttctcgtac ggtttttcga tgccatagcg acgcataact
     3661 gtctggattg gttctgccag cacttcccag ttgtgatcca gctcatccag cagatggtca
     3721 cggttcactt ccagtttgct cacgcctttc agggtggatt gatacgcaat cagcgcataa
     3781 ccgataccca cgccgaggtt acgcagcacg gtggagtcgg tcaggtcacg ctgccagcgg
     3841 gaaaccggca gtttgcttgc cagatgctgc aataccgcgt tggaaaggcc cagattccct
     3901 tcggagtttt cgaagtcgat cgggttaact ttatgcggca tggtggaaga accaatctca
     3961 ccagcaatgg ttttctgttt gaagtggtta agggcgatat aaccccagac gtcacggtca
     4021 aagtcgatca gaatggtgtt gaagcgcgca acgcaatcaa acagttcggc aatgtagtcg
     4081 tgcggttcaa tctgggtagt gtacgggttc cattgaatac ccagcgaggt gacgaactct
     4141 tcgctgaact gatgccagtc aacttccggg taagcggcga tgtgggcgtt atagttaccg
     4201 accgcgccgt tgattttgcc gaggatctcc acctggttaa gctggcggtg ctggcgctcc
     4261 atacggtagg cgacgtttgc catctcttta ccgatggttg acggcgtggc tggctgaccg
     4321 tgggtacgag acagcagcgg gatatcgcgg tactgaacgg cgagatcttt aatgccatca
     4381 atcagttgac gccagtacgg caggatcact tcatcacgcg cggttttcag cattaatgcg
     4441 tgggagaggt tattgatatc ttccgaagta caggcaaagt ggatgaattc agaaaccgcg
     4501 tgcagttccg ggatctccgc cactttttct ttcaggaaat actcaaccgc tttaacgtcg
     4561 tggttagtgg tacgctcgat ggttttgatg cgtgcggcat cttcttcgct gaaattggcg
     4621 acaattgcat caaggtaacc gattgcgtcg gcagcaaaag caggaacttc cttgatcgct
     4681 gcgtgcgcgg ccagtttttg cagccaacgt acttcaactt gtacacggaa tttcagcaaa
     4741 ccatattcgc tgaaaatccc gcgcagcgcg ctgagtttat cgccgtagcg tccatcgaca
     4801 ggggaaacgg cggtcagtga ggataattcc atagatcaca actccggggt taaatgagca
     4861 agaatttgtt ttgcctgagt ggtcaggcga ttacgagaaa acatcagttg tagacgtccg
     4921 ccgccgacct ggtgccagag cacggcggcg cgaatgcctg ccagcagggt tgcgcgaact
     4981 ttcgcctgca cttgtgggct ttgcagtaca gcaggggaac cggtgacctg aatgcgcggg
     5041 ccaagcgggc tgatcacatc aacatagata gcagccatcg cgctcatcag cgtttcggac
     5101 tgtaaatcga agtgttcgag ctggcgttgc aggccgttga tccggttgcc cagagtgtcg
     5161 agcgcgcctt tcgctgagga gagtttgcgc tcaagcacca tcaagctgag tgtgtagcgg
     5221 gttaattcgg cgtttaagcc ctggcaactg ctggcattga gcacgccgag cagggtttcc
     5281 agcccgacgc gcaggttggc ttcgctaccg ccaaaaaccg ccagcgtcga gctggggttc
     5341 atatcaataa tactgttgag tgagacgtgt agcgcatcgg catcacaatg cccctggtga
     5401 gcgagttgtt gcaccaggcg tgccgactga caaataccgg ccagggcgag ggtgatgtca
     5461 tagtaattct ttgccacgtt cactgcttcc ttgttttaag taaagataat aatcagaccg
     5521 gcagcggcag acgctgctca ataataccgc caccgaggca cacttcaccg ttatagaaga
     5581 cggcagactg gcccggcgtc acggcggcaa ccggctcatc gaaaatcact ttaatgcgat
     5641 cgtcgtccag cgccttgacg gtgcacggaa tgtcggtctg gcgatagcgg gtttttaccg
     5701 tgcaacgcat agtgccggtg aatggttcgc gatcgaccca gtgcagctgc tgggcaatca
     5761 acccgacaga catcagccgc gggtgttcat ggccctgagc gacaaccaga atgttgtttt
     5821 cgacgtcttt gtccaccaca taccacggtt cttcggtgcc ttctttggtg ccaccgatac
     5881 ccagaccttt acgctgaccg agagtgtgat acatcagccc ctggtgctca ccaatttcat
     5941 ggccatcgac ggtaatgatt ttgcccggtt gcgccgggag ataacggccc aggaactcgc
     6001 ggaatttacg ttcgccaatg aagcaaatgc cggtagagtc ttttttcttc gcggtgacca
     6061 gaccaagatc ttcagcaatc ttacgcacct gcggtttttc cagttcgccg accgggaaca
     6121 ggctttgcgc aatctgctca tggctgagcg tataaaggaa gtagctctgg tctttattgc
     6181 tgtccagacc acgcagcagg cggctcttgc catcgacatc ggcccgacgt acgtaatgac
     6241 cggtagcgat ataatcggca cctaaatctt cggcggcaaa ttcgaggaag gctttaaatt
     6301 tgatctcctt gttgcacaga atatccggat tcggcgtgcg accggcttta tattcggcaa
     6361 ggaacagttc gaagacgttg tcccagtact cggcagcaaa gttaacggtg tgcagttcaa
     6421 tgccgagctt gtcgcagacc gcctgggcat cagccagatc cgctgccgct gtgcaatatt
     6481 cctcaccgtc gtcttcttcc cagttcttca taaacaggcc ttcgacctga tatccctgtt
     6541 gttgcaacag ccaagcagaa acggaggaat cgacaccgcc ggacatgccg acgattactt
     6601 tttttgcggt ttcagacatt ggatcactca cgacattgaa cttcaaggcg gcgtattcta
     6661 tcacgcagcc ttttagatga caccctttgt aaaaggccag ttaaaatcgc caatcatctc
     6721 cagcggataa cgttgcccgc tttgataaca acgaatactt tccgccacca gcggcgaacg
     6781 aagatttgac gcctgtaaaa tttcttcggc gctgacccaa cggcagcagt cgatatcgct
     6841 gtcatgaggc tgggtcgggc atatttgctc aagctcaatg gcaaagagga aacgcaaaaa
     6901 cggcgtttta tctggcgcaa tccactgatg catacgaata aagtgttgcg gctgcgcgct
     6961 gatgccggtt tcttcccaca gttcacgggc ggcggcttcc actaaggttt catcggcttc
     7021 cagatgcccg gcaggttggt tccataacgc tttaccatta atcgtctctt caacgactaa
     7081 aaatttgcct tctgcgtgca ccacgcaagc aacggtaacg tgcggtttaa acattttgca
     7141 tccttaatct gtcacttctc gccattcgcc attggcaaga ttatccaaag agtaatctcc
     7201 catcgcatag cgaatcagtc gcagcgtggg gaaaccaaca tgggcggtca tgcggcgcac
     7261 ctggcgatta cgtccttcat ataaggtgat cttcagccag ctggtgggaa tacttttgcg
     7321 ttcacgaatt ggtggattcc gcggccataa ccacgcgggt tcgtcaacca gctccgcgcc
     7381 tgcgggcagg gtagggccat catttaaggt tacgccattg cgcaaggctt caagtgcgtc
     7441 ttgtgtggga ataccttcca cctgcacata atagattttt ccggtgcgtt tacccggctg
     7501 ggttaaacgc gcctgcaacg cgccgttatt ggtcagcacc agcaaccctt cgctatcgcg
     7561 gtcaaggcga cctgctgcat aaacgccctg aaccgggatg aattctttta atgttttgcg
     7621 tccggcttca tcggtgaact gcggaagaac atcgtagggt ttattgaaga ggatcacacg
     7681 cgtgggctgg ttttcatgtt tacgcctggt agaacgttgc gagctgaatc gcttaacctg
     7741 gtgatttcta aaagaagttt tttgcatggt attttcagag attatgaatt gccgcattat
     7801 agcctaataa cgcgcatctt tcatgacggc aaacaatagg gtagtattga caagtcaatt
     7861 acaaatcatt aacaaaaaat tgctctaaag catccgtatc gcaggacgca aacgcatatg
     7921 caacgtggtg gcagacgagc aaaccagtag cgctcgaagg agaggtgaat ggaaagtaaa
     7981 gtagttgttc cggcacaagg caagaagatc accctgcaaa acggcaaact caacgttcct
     8041 gaaaatccga ttatccctta cattgaaggt gatggaatcg gtgtagatgt aaccccagcc
     8101 atgctgaaag tggtcgacgc tgcagtcgag aaagcctata aaggcgagcg taaaatctcc
     8161 tggatggaaa tttacaccgg tgaaaaatcc acacaggttt atggtcagga tgtctggctg
     8221 cctgctgaaa cccttgatct gattcgtgaa tatcgagttg ccattaaagg cccgctgacc
     8281 actccggttg gtggcggtat tcgttctctc aacgttgccc tgcgtcagga actggatctc
     8341 tacatctgcc tgcgtccggt acgttactat cagggcactc caagcccggt taaacaccct
     8401 gaactgaccg atatggttat cttccgtgaa aactcggaag acatttatgc gggtatcgaa
     8461 tggaaagctg actctgccga cgccgagaaa gtgattaaat tcctgcgtga agagatgggc
     8521 gtgaagaaaa ttcgcttccc ggaacattgc ggtatcggta ttaagccgtg ttctgaagaa
     8581 ggcaccaaac gtctggttcg tgcagcgatc gaatacgcaa ttgctaacga tcgtgactct
     8641 gtgaccctgg tgcacaaagg caacatcatg aagttcaccg aaggcgcgtt taaagactgg
     8701 ggctaccagc tggcgcgtga agaatttggc ggtgaactga tcgacggcgg cccgtggctg
     8761 aaagttaaaa acccgaatac cggcaaagag atcgtcataa aagacgtgat tgctgatgca
     8821 ttcctgcaac agatcctgct gcgtccggct gaatatgatg ttatcgcctg tatgaacctg
     8881 aacggtgact acatttctga cgctctggca gcgcaggttg gcggtatcgg tatcgcccct
     8941 ggagcaaaca tcggtgacga atgcgccctg tttgaagcca cccacggtac tgcgccgaaa
     9001 tacgccggtc aggacaaagt aaaccctggc tctattattc tctccgctga gatgatgtta
     9061 cgccatatgg gctggactga agccgcagac ctgattgtta aaggtatgga aggcgcgatc
     9121 aacgccaaga ccgtaaccta tgacttcgag cgtctgatgg aaggcgctaa actgctgaaa
     9181 tgttcagagt ttggtgatgc gatcatcaag aatatgtaat cactacatgt gttaaatatt
     9241 taaacgggcg tataacacgc ccgttgtttt atttatgtgg atattattaa tagcatatcg
     9301 agcatattta tataaaacct attacttgag cccatatggg catattttta taatacaact
     9361 cttatgcaaa catttatttg ttatttcgct ttctcctgga ggacactctt gactgctttt
     9421 gagtaaactc cataaattct tgttgaatgg tgcaatgtta taaatagtaa taggatattc
     9481 tttttcctta aggataatac caagcttaac cggtgtaaat atccttccag gaggaagaaa
     9541 tatagtagat tgataccaga tgatcatttt catatcaccc catatggctg aaaaagatat
     9601 gccgcatgta ggttgaatta ccgtgtcaat tactacccac ttcatttgtt atgtcttatc
     9661 ccacggtatt taatatagtt catttggatg ttcatttctt tattttgcat atgagtatat
     9721 taccccttca aaaaataaat taattaaaac gattgcttat ataaaacaaa atttaaatca
     9781 aggaatctca atggatgtta aacaaaatga gatttagtga aaacaataaa ttattcactt
     9841 cgttttagat ttgtttagct ataatgttat acattcaaat gactgaacat cctgtaatta
     9901 aaacatagcc tttatgctac ttggtgccaa tttgctaaac attatggttg cctttttata
     9961 taacgataat aatgaatata aacatgacat gagaataagg tttcaatttt tgagttatat
    10021 aggaaaggtt taacctgttc ctggctaaaa tacatataac tggatgatga ctaaaccaaa
    10081 acacatatgc gttaagtatt gaaacgggcg tatggcacac ccgttgtttt tataaatata
    10141 ttaaccgtta taaaataacg tataaaaagt caagtgatca catttcaaat atcaatttga
    10201 tagtatgggc ttggtgatta tttatgggta gcaataaaag gacagtattt atcatccata
    10261 gggatagtct ctgtactttt attcccatta tgctaatgcc ttactgaatt atgaagcatt
    10321 tcttaagcat ccaactttag ctagattaat ggtttattat tttctacatc ttcaatatat
    10381 aaaagcgtat tatcaatggc gtagtaactg cgtttgttat gattaacatc agtaacccaa
    10441 cggaaaacgc ccgcgcctgc tagtgttgaa cagtattccc gaaatgtaga ttttccgcaa
    10501 atatgaagca atgcggcctc ttttatttta gcaggattct tggtcgtact aacttttaac
    10561 tggttcctgg ttcctcttaa taacaaaacc gtgtcatcgt gagtaataat tctgatgtta
    10621 tccgtagcca gataataaat gtaatgtgca atacggtgat gttttaattc tgaataaaac
    10681 caggagaagt tttgctcttt tctcacttgc tcaaacatct tttgaaaaac aacgacctga
    10741 tccatcagga taataacctc ttgttagttg tgagactgcg tagtgtgcac gatcggtttt
    10801 accacttcaa tctggtctgt cctttggctg tgatatgtac agagtgtgat agagggatta
    10861 cctgaattct cccggtgagc attttgcaac ggaccagctc cggtacaaat gctgttgtgg
    10921 gttcagatta taacattctg tctaaggggc gggataaagg tgaaattagg gggcatgaaa
    10981 gatgacttta taaccttgct caccccaatg ttgtaaaagt tcgttttgcc ttctcgttgg
    11041 tgccatgcct gtccagacaa tcaatgtttg cgtcgggaac agttccgggc gcggcgattc
    11101 aatgggatca gcaagaacag aaatgtgcca tcctgacaat gataaacgcc atgcttccag
    11161 ccacaatcgt gctctctctt caacattcca cgccatcaga atggcttctt taccgggctt
    11221 acggcgcgtt tcgaaaagcg aggttgctgc gtattcaatt aatgcgccgt caaacatact
    11281 gctcataatg cgggaggtgt tgtgatcaag cacgagacgc tggcgaacag gaaggtaaac
    11341 atgattaatc aattgatcta ctgggtactc tcgacccagt gaaataattc tcgcgcgtag
    11401 tttggcagga ttagccatac gaagaattga catcatctct tcttgcaggc ggctccagtc
    11461 atcttccgta tcctggctgg tggtttccag taatgcttta actttgccta cagggacgcc
    11521 attactgatc caacgcttga tctcttcgat gcgttgtatg tcttcttcat caaagagtcg
    11581 gtgtccgcct tcactgcgct gtggttttaa caaaccgtag tggcgttgcc aggcccggag
    11641 agtgacagga ttaatcccgc aacgttcagc aacatcacca atgctgtaat aagccacaat
    11701 tcctccttgc ggtcacaaat ctccgtcgcc ggtacacgac ccaataatac tttgtacaat
    11761 atacgctaaa attgtacaaa gtataaataa gatttcagct aaattggatg aaacattatt
    11821 tttaatgtag attaaattta aacataacgt attcattttc acgatgattt actgaaatca
    11881 tgtgaaagaa tgtgctgaaa attatttttt ctctggccac gctatggaag ggataccatt
    11941 caatttagct ttagcaaaca gatctccctg aaacatctca attcctgcgg attcaagcca
    12001 catccactct tctggtgttg ccacgcccat agcactgact tgaatttcaa gtgatgtaca
    12061 gcattttatg atcgcttgaa taattgcctg ccgtggcccg cttttatgaa cattggtaat
    12121 caattcctga ctgattttaa ttctgtcagg ctggaagcgt gacaggagta acaaaccggc
    12181 aaaacctgcg ccaaaatgat caattgctac actgataccg gcagccttaa gcgatttaat
    12241 cgcttctgca aactcatcaa accgagatat aacttcactt tcagtaaatt caacgatgat
    12301 ttgttcaggc accagagcat tggcctttat ttcatcaagt aaaaaagaga ctgcgtcagg
    12361 ttcgttaacc agggtcatag gtaatagatt gattgaaatc attttatcac cgagctcaag
    12421 tgcgtgtgtc atcgcgaatg caagcgcctt acttttgaga tccgctgtgt agatttcccc
    12481 gtctttacgc tgcccaaccg ctatggctga tgggctatct tcattttttt gcacaatggc
    12541 ttcaaaagcg attatccgcc gcgataaggg atcgacaata ggatgaaagg caaagtggtc
    12601 gtttatagtt ggtgaaaggg tacaggaatc aagttcttta tcagatccgt cagcgataaa
    12661 aagccaggag tcttcggcag ggatctcgaa ataggttgat tgttcggttg caaggacaaa
    12721 agtacgaaaa aattgtagcg ctctgtcatc ataagttagc tgaaattttg atgtgccttt
    12781 gtcgaatacg gcctgtaaaa cgtcatctcg ctcgtgcagg cgcaaatcaa ataattccat
    12841 tcccgctttg ccaaaacggc gagcaggcgc gtaatcgcac agcagctcaa caatattata
    12901 gtgccgtgga tcttggcata tagcccgata tatcatttta acctgttctt ccggaccttc
    12961 cagaagctgg aaaaaatgag aaccattaaa cagtaagatc cctgttacgt cagactgcat
    13021 gttcctgcga tttgctatcg aaaccatttc ttcgattttt ttgacaggtt cgtcgtcacg
    13081 tatatggcta cgataaataa gggtggtaag cattaacaat ccagggtaat gggtgaggcg
    13141 agagtaagac ggtaacagac atatcttctt gtgtctttct tttaatacca aaacataacc
    13201 gtttctttac attgataaaa aatggaaaaa gttgaacacg agttggcgaa aaatcttgta
    13261 tagattgtca gttaaatgat gcaatatgtt ttatcataac acattgtttt atatacatta
    13321 gtactaattg caaaaaatta atttatcatt ctgtacacat atttcgtaca agtttgctat
    13381 tgttacctca cttcacattg attaacattt ttaacagagg cgtagcatgc atcaaaattc
    13441 agtgacttca gattctgcgg gagcaatcac tcgttacttt gcaaaagcta acttgcctac
    13501 tcagcaggaa actctcgggg aaattgtgac tgagattttg aaagatggac gtaatctgag
    13561 tcgaaagtcg cattgtgcca aacttctttg ccgactggaa caagcgacag gggaagagga
    13621 acagaaacat tataatgcac taattgggtt gctttttgaa taatgcaaca gcctgattat
    13681 ttaacaggtt agctatgaag tcgttatgaa gacatctgat aatgaacgta taaaatatga
    13741 aattactggc caggcggtgc tccagatact gcgcatgaag ataaattttt cattgcagac
    13801 tcttattaag caattactcg taatgaaatc ggctgaggaa gatgctttcc gacgcgattt
    13861 aattgacagc ataattcgtg attttagcaa tagtgattca ggagggccaa accgaagaac
    13921 agcaacagct gacaataaaa gtatgttcaa tggtaagaaa ataaacagaa tacattaaaa
    13981 tttcataagt aagatgagag gttaccatgc ttgaagatac tacaattcat aatgcaataa
    14041 ctgataaagc gttagcaagt tactttcgca gttcgggtaa tttgttagaa gaagagtcag
    14101 cagtgttagg gcaggctgtc accaatttaa tgctttcagg cgataatgtt aataataaaa
    14161 atattatctt aagtctgata cactccctgg aaacaacaag tgatattctc aaagctgatg
    14221 tgattagaaa aacactggaa atcgtgttgc gatacacagc tgatgatatg taacttctaa
    14281 ttattaagta taagtttata gaaaactcat tcatcttatt tttgtctgtc gctttagact
    14341 atacacagca taattttatt gggttaatat ttctacgagg ctgacatgaa taattcaatc
    14401 ccagagagat ttatttttca atgtgctttg tttaaaaatc tcgagaggga ggtgttcatg
    14461 acgcatgggt atgttgatag tcatattatt gatcaggctt tacgcttgcg tttaaaagat
    14521 gaaaccagtg tgattctttc tgatctctat ctgcaaatat tgcagtacat tgaaatgcat
    14581 aagactacac taacggatat cattattaat gacagggaat ccgtgctctc ttagaacttg
    14641 agtaaggttt tagaataaac atcagctgta tcaccatgct gatgcaaagt gagcatttca
    14701 ggcgttatgc tttcttatta tgtccgcaat atcaggtgtc aagaatggag agttctcgct
    14761 ctccattctt gacgcctgat atcccgccta acttattttg tactgcctac attatgctca
    14821 ataattaatg ggttagatca agatggagaa aattttatct tcggcgtgga tatttcttat
    14881 gctctctacc atgcatctct gtagatgcca gcgcgagcga atgaatgg
//
LOCUS       DADLTF010000100        14925 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.100, whole genome shotgun sequence.
ACCESSION   DADLTF010000100 DADLTF010000000
VERSION     DADLTF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.100"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            597..794
                     /locus_tag="JN749_002847"
     CDS             597..794
                     /locus_tag="JN749_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369204.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="JN749_002848"
     CDS             complement(744..1937)
                     /locus_tag="JN749_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7369205.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="JN749_002849"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="JN749_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HAY7369206.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="JN749_002850"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="JN749_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HAY7369207.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="JN749_002851"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="JN749_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HAY7369208.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7800
                     /gene="iucD"
                     /locus_tag="JN749_002852"
     CDS             6484..7800
                     /gene="iucD"
                     /locus_tag="JN749_002852"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HAY7369209.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7806..10001
                     /gene="iutA"
                     /locus_tag="JN749_002853"
     CDS             7806..10001
                     /gene="iutA"
                     /locus_tag="JN749_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HAY7369210.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            10945..14832
                     /gene="sat"
                     /locus_tag="JN749_002854"
     CDS             10945..14832
                     /gene="sat"
                     /locus_tag="JN749_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HAY7369211.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSTTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4020 a         3554 c         3707 g         3644 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatggctcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg tcgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagaa gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccc tgcttcttta actcactata cagcaaatat tcaggaataa
     7801 cgatgatgcg caaaaagtat atgccccggg ctcttggtcc gctgcttctt gtcgtgctgt
     7861 caccagctgt cgcccagcaa aacgatgata atgagatcat agtgtctgcc agccgcagca
     7921 atcgaactgt agcggagatg gcgcaaacca cctgggttat cgaaaatgcc gaactggagc
     7981 agcagattca gggcggtaaa gagctgaaag acgcactggc tcagttaatc cccggccttg
     8041 atgtcagcag ccaaagtcga accaactacg gtatgaacat gcgtggccgc ccgctggttg
     8101 tcctgattga cggtgtgcgc ctcaactctt cacgttccga cagccgacaa ctggactctg
     8161 tcgatccttt taatatcgac catattgaag tgatctccgg cgcgacggcc ctgtacggtg
     8221 gcgggagtac cggagggttg atcaacatcg tgaccaaaaa aggtcagccg gaaaccatga
     8281 tggagtttga ggctggcaca aaaagtggct ttaacagcag taaagatcac gatgagcgca
     8341 ttgccggtgc tgtctccggc ggaaatgacc atatctccgg acgtctttcc gtggcatatc
     8401 agaaatttgg cggctggttt gacggtaacg gcgatgccac cctgcttgat aacacccaga
     8461 ccggcctgca gcactccaat cggctggaca tcatgggaac cggtacgctg aacatcgatg
     8521 aatcccggca gcttcaactg ataacgcagt actataaaag tcagggagac gacaattacg
     8581 ggcttaatct cgggaaaggc ttttccgcca tcagcgggag cagcacacca tacgtcagta
     8641 aggggctgaa ttctgaccgc attcccggca ctgagcggca tttgatcagc ctgcagtact
     8701 ctgacagtga tttcctggga caggaactgg tcggtcaggt ttactaccgc gatgagtcgt
     8761 tgcggtacta cccgttcccg acggtaaatg cgaataaaca ggcgacggct ttctcctcgt
     8821 cacagcagga taccgaccag tacggcatga aactgactct gaacagccaa ctgatggacg
     8881 gctggcaaat cacctggggg ctggatgctg agcatgagcg ctttacctcc aaccagatgt
     8941 tcttcgatct ggctcaggca agtgcttccg gagggctgaa caaccataag atttacacca
     9001 ccgggcgcta tccgtcatat gacatcacca atctggcggc cttcctgcaa tccagctatg
     9061 acattaatga tatttttacc gttagcggtg gcgtacgcta tcagtatact gagaacaggg
     9121 tagatgattt catcgactac acgcagcaac agaagattgc tgccgggaag gcgatatctg
     9181 ccgacgccat tcctggtggt tcggtagatt acgataactt tctgttcaat gctggtctgc
     9241 tgatgcacat caccgaacgt cagcaggcat ggttcaattt ttcccagggg gtggcattgc
     9301 cggatccggg gaaatattat ggtcgcggca tctatggtgc agcagtgaac ggccatcttc
     9361 ccctgacaaa gagcgtgaac gtcagcgaca gtaagctgga aggcgtgaaa gtcgattctt
     9421 atgaactggg ctggcgcttt atcggtgaca acctgcggac tcaaatcgcg gcatattact
     9481 cgctttccaa taagagcgtg gaaaggaata aagatctgac catcagtgtg aaggacgaca
     9541 ggcgccgtat ttacggcgtg gaaggtgcgg tggactacct gatcccggat actgactgga
     9601 gtaccggtgt gaacttcaat gtgctgaaaa ccgagtcgaa agtgaacggt caatggcaaa
     9661 aatatgacgt gaaggaatca agtccatcga aagcgacagc ttacattaac tgggcgccgg
     9721 aaccgtggag tctgcgtgta cagagcacca cttctttcga cgtaagcgat gcagagggta
     9781 acgatattaa tggttacact accgtcgatt ttatcagtag ttggcagctt ccggtgggaa
     9841 cactcagctt cagcgttgag aacctcttcg accgtgacta taccactgtc tggggacagc
     9901 gtgcacctct gtactacagc ccgggttacg gccctgcttc actgtacgac tacaaaggcc
     9961 ggggccgaac ctttggtctg aactactcag tgctgttctg acggggatgc ttttgcaaca
    10021 agggtatgct gtaaacaaca tggtccttgg tggccatgtt gtttacagcc agtataatat
    10081 acagccttaa caagatattc cgtctccgga atgggctgtt caccactgaa taacattatg
    10141 tctgttttat ggatctttga ctgaaattta tcgcatatcc tcatttcatt gtccgtctga
    10201 aaactaacct tacaagtaag cgtcgtctca gcaccgtctg gcagatcctg aaattcctga
    10261 gaggatgatc tgcgcccaat atcctgagcc ggaactttgc tgcaatgaag gccaacgaaa
    10321 aatgggggag tatgttatag agtacttgct acagggtaaa aagaggtacc tgtcgctggt
    10381 tctccgttca gtttaaccgg gagattttct cctgcgcctg tcataaagtc cggtaatgga
    10441 gattgttaat acgatggtgc acgatgcatc gtcagtgccc ggcccggagg atgccccgta
    10501 gctacatacg gatcagggtt ggcaatatcg aatggcaggc tataggcaaa gttaaagccc
    10561 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggag
    10621 aacttcttcg gcacactgca aaatcgcatc gtggtcgatc gctaattcgt cgtgaatcac
    10681 tgaacgatgt tccatgcgaa cagatattcg atcatatcga aatattaaac attacccctc
    10741 cccacattgg gcaaaaaaaa acaaaaaacg atcaattaac gatcaattag tattgattca
    10801 cataatctct catcattaat gagaacattg tcacatattt acaatataga actgttactt
    10861 ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata ctttgttcat
    10921 tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata tagtgctgcc
    10981 actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa aacaaaccga
    11041 aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc agcaaatatt
    11101 gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa aggtattttc
    11161 cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt ctctttccat
    11221 aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac cgcaatagga
    11281 ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc agaaactcag
    11341 gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga ttttgagatt
    11401 cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga gaccaaccct
    11461 acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg ttcaaaaaaa
    11521 atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga atccaaaatc
    11581 tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga ggtcacccaa
    11641 tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata
    11701 ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta
    11761 gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa
    11821 tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat
    11881 ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc
    11941 actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat
    12001 gttctgttta aggataattt ggattttggt agcggtggta ttatctttga cgaaggccat
    12061 gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga tatcggaaaa
    12121 gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc
    12181 cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat
    12241 gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag
    12301 gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact
    12361 aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc
    12421 actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt tctggcaatc
    12481 aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac
    12541 aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac
    12601 acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca tgcaacagag
    12661 catgcaatat tcagaagcac aacgagtcat tgctccctgg tatttctttg tggaacggac
    12721 tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac
    12781 aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa
    12841 tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa
    12901 ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat
    12961 aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact
    13021 aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata
    13081 gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct
    13141 ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt
    13201 attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta
    13261 acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc
    13321 attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt
    13381 actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca
    13441 gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct
    13501 acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta
    13561 gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca
    13621 gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct ggcccaagca
    13681 gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca accttctgtt agtcgacttc
    13741 attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc accaaaagga
    13801 actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga tgtaacaccg
    13861 gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg ctataaatct
    13921 gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg ctataaagcc
    13981 ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga cattaacggt
    14041 gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg tggattcagt
    14101 gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga tggccttgac
    14161 ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga tgccttcagt
    14221 ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga gtccggagca
    14281 tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc aactttcgcc
    14341 ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga agtcggttac
    14401 cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt ttacggtgct
    14461 gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat gaaggataag
    14521 gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt ctccggtaag
    14581 gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt tgccaacggt
    14641 gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa agacggtcgt
    14701 atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt cggtcttgag
    14761 tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc caacttccgt
    14821 tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg gagagaatat
    14881 gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DADLTF010000101        14845 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.101, whole genome shotgun sequence.
ACCESSION   DADLTF010000101 DADLTF010000000
VERSION     DADLTF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14845
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.101"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..434)
                     /locus_tag="JN749_002855"
     CDS             complement(<1..434)
                     /locus_tag="JN749_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7369212.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTG"
     gene            442..942
                     /gene="tnpA"
                     /locus_tag="JN749_002856"
     CDS             442..942
                     /gene="tnpA"
                     /locus_tag="JN749_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAY7369213.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(981..1328)
                     /gene="grxD"
                     /locus_tag="JN749_002857"
     CDS             complement(981..1328)
                     /gene="grxD"
                     /locus_tag="JN749_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HAY7369214.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1663..2490
                     /locus_tag="JN749_002858"
     CDS             1663..2490
                     /locus_tag="JN749_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HAY7369215.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2618..3199
                     /gene="sodB"
                     /locus_tag="JN749_002859"
     CDS             2618..3199
                     /gene="sodB"
                     /locus_tag="JN749_002859"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HAY7369216.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3345..4514)
                     /locus_tag="JN749_002860"
     CDS             complement(3345..4514)
                     /locus_tag="JN749_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7369217.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="JN749_002861"
     CDS             complement(4680..4769)
                     /gene="ynhF"
                     /locus_tag="JN749_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HAY7369218.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5068..6093
                     /gene="purR"
                     /locus_tag="JN749_002862"
     CDS             5068..6093
                     /gene="purR"
                     /locus_tag="JN749_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HAY7369219.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6090..7022)
                     /locus_tag="JN749_002863"
     CDS             complement(6090..7022)
                     /locus_tag="JN749_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7369220.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7135..8346
                     /locus_tag="JN749_002864"
     CDS             7135..8346
                     /locus_tag="JN749_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HAY7369221.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8637..9785
                     /gene="cfa"
                     /locus_tag="JN749_002865"
     CDS             8637..9785
                     /gene="cfa"
                     /locus_tag="JN749_002865"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HAY7369222.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="JN749_002866"
     CDS             complement(9825..10466)
                     /gene="ribE"
                     /locus_tag="JN749_002866"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HAY7369223.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10681..12054
                     /gene="mdtK"
                     /locus_tag="JN749_002867"
     CDS             10681..12054
                     /gene="mdtK"
                     /locus_tag="JN749_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HAY7369224.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12095..13351)
                     /locus_tag="JN749_002868"
     CDS             complement(12095..13351)
                     /locus_tag="JN749_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369225.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLKTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13659..13735
                     /locus_tag="JN749_002869"
     tRNA            13659..13735
                     /locus_tag="JN749_002869"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13693..13695,aa:Val,seq:gac)
     gene            13740..13816
                     /locus_tag="JN749_002870"
     tRNA            13740..13816
                     /locus_tag="JN749_002870"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13774..13776,aa:Val,seq:gac)
     gene            13924..14229
                     /locus_tag="JN749_002871"
     CDS             13924..14229
                     /locus_tag="JN749_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HAY7369226.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14355..>14845
                     /locus_tag="JN749_002872"
     CDS             14355..>14845
                     /locus_tag="JN749_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAY7369227.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATD"
BASE COUNT         3640 a         3823 c         3524 g         3858 t
ORIGIN      
        1 cccgtgacct gacggctgtt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcagcatt ctgtccccgc
      121 tttttgaatc ggggaaaagc agcccgcttc cggaagaagt ttttgtaagc ccgctcaagg
      181 tctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg
      241 gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttatttccg
      301 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc
      421 tgtaatcgtt tcacaggtca aatgatactt tggtctgtga aaaaagaaac cgatatccgg
      481 cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga
      541 cgccagattt ttgaccatga cacgacagaa aaactacgca cttacttttc aaatgtatgt
      601 gctgattttg cagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta
      661 attaactatc ctcccaaact ggcgatatcc agtctggtaa acagcctcaa aggcatatcg
      721 aataggttac tgcgacgaga tcgaccagat attgcagtca ggtattacta caaaggcgtt
      781 ttgtggagtc ctggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc
      841 caatacattg aacaacagca aacacctggt caggtggaaa accgcgcctt atatccccgc
      901 cctgaaggac gaggttttac ggcgcaccgg ataaaggact gacggatgcc cgttcgcatc
      961 cgtcagtatt gcaggacgga ttattccgcg tccggctctt cagacttgta tttagcggca
     1021 gtttctttga tcagctgctg cagttcgcca cgctgataca tttcgatcac gatatcacaa
     1081 ccgccgacca gctcgccgtc aacccacagt tgcgggaagg tcggccagtt agcatatttc
     1141 ggcagttccg cacgaatgtc cggattctgc agaatatcaa cataggcaaa acgttcgcca
     1201 catgcggcaa gcgcctggac tgcctgggca gagaaaccgc agctcggcag tttcggtgaa
     1261 cctttcatgt acagcaggat cgggttttca gcaatctggc gttggatttt ttcgatagtg
     1321 gtgctcattg tcttgcttcc tcaaacttct ttacggcagt aatctgacat tgtagcgggt
     1381 cagtgcggca tcggaaaata acattttcat cacgcttttg ctattttatc cctttgctct
     1441 atttgttgca tttcaaatat tcgtttcttc tctgatgcgc ggcaaactgg cgtatttata
     1501 accattttta gctttcactg ctgcattttt ttgcgctcgc caacgaaacg tattttttaa
     1561 caataaaagc tattaacttt ctcttcttct atgcattaga atcatcaagt tttgtaaatc
     1621 agacgcaggc atgatagacc tgccttaaca gagggacgct cagtggcgcg gataaaccgt
     1681 atttcgatca cgctctgtgc tttacttttt accaccctgc ctttaacgcc tatggcccat
     1741 gcttcaaagc aagccaggga gagttctgct accgctcata tcaccaaaaa agcagataaa
     1801 aagaaaagca cggcaaccac caaaaagacc cagaaagcca gccagaaaac agcgaaaaaa
     1861 gccgccagta aaagtacgac caaaagcaaa accgcttctt ccgttaaaaa atcttccatt
     1921 accgcttcta aaaacgccaa aactcgcagc aaacacgccg tcaataaaac ggcctcagcc
     1981 agctttaccg aaaagtgtac caaacgtaag ggctataaat cgcattgtgt gaaagtcaaa
     2041 aatgccgcgt caggaaccct tgccgacgcg cacaaagcga aggtgcaaaa agccacgaaa
     2101 gtggcaatga ataaactgat gcagcaaatt ggtaagccat atcgttgggg cggcagctca
     2161 ccgcgtaccg gttttgattg cagcggcctg gtttattacg cttataaaga tttggtgaaa
     2221 attcgtattc cgcgtacggc gaatgaaatg tatcacctgc gtgatgcagg acctatcgaa
     2281 cgtagtgagc tgaaaaacgg cgacctggtc tttttccgta ctcagggacg cggtacagcc
     2341 gatcatgtcg gcgtgtatgt cggcaacggc aaatttattc agtcaccgcg cacaggtcag
     2401 gaaattcaaa tcacttctct cagtgaagac tactggcagc gccactatgt tggcgctcgt
     2461 cgggtaatga ccccaaaaac acttcgctaa aactttaccc tgttgttacg gcaacagggt
     2521 aagttcatct tttgtctcac cttttaattt gctaccctat ccatacgcac aataaggcta
     2581 ttgtacgtat gcaaattaat aataaaggag agtagcaatg tcattcgaat tacctgcact
     2641 accatatgct aaagatgctc tggcaccgca catttctgcg gaaactatcg agtatcacta
     2701 cggcaagcat catcagactt atgtcactaa cctgaacaac ctgattaaag gtaccgcgtt
     2761 tgaaggtaaa tcactggaag agattattcg cagctctgaa ggtggcgtat tcaacaacgc
     2821 agctcaggtc tggaaccata ctttctactg gaactgcctg gcaccgaacg ccggtggcga
     2881 accgactgga aaagtcgctg aagctatcgc cgcatctttt ggcagctttg ccgatttcaa
     2941 agcgcagttt actgatgcag cgatcaaaaa ctttggttct ggctggacct ggctggtgaa
     3001 aaacagcgat ggcaaactgg ctatcgtttc aacctctaac gcgggtactc cgctgaccac
     3061 cgatgcgact ccgctgctga ccgttgatgt ctgggaacac gcttattaca tcgactatcg
     3121 caatgcacgt cctggctatc tggagcactt ctgggcgctg gtgaactggg aattcgtagc
     3181 gaaaaatctc gctgcataat aactgatggc aaatgcagca ttgcctgatg cgcttcgctt
     3241 accatgccaa cgcggatgag gcgttaacag cgcattcggc aagataaatc gcacgttgtc
     3301 agcaactgta acgcagaagg ttatccttct gcgtttttgt ttaattagct gttagcaacg
     3361 caaactgttt caggttgttt tctggctgac ataaacacca gcaataatgc cagtcccgcg
     3421 acaatcgccc ccgtcaccgg cacaaagctg tatcccagcc cagcggaaat taccgcacca
     3481 ccagcagctg ctcccagcgc atttccaaga ttaaaggcac caatattgac tgatgaagac
     3541 agacctggcg cttcactggc gacatgcatc acgcgcatct gtaacggagg tacaaccgca
     3601 aaggttgctg cgccccacac caccatgcta atagctgcgc cgaactcatt gcgggccagg
     3661 aacgggattg ccagcataat caccatcagc agcaacaaaa agcctttcaa cgtgccgtta
     3721 actgaacgat ctgccagttt gccgccgaga tagttaccga tagagaatcc gacaccaatc
     3781 agcaccagca ttgccgtgac gaacaccggt gttgcgtggg taatactttg cagtaccgga
     3841 gagatatagg tgtagagggt aaacattgca ccagctccca gtaccgtcgt cagcaatgca
     3901 gacagcacct gcggacgcat taataccgcc agctcttttt tcacttcagg tcgtgcccct
     3961 gtaccacctt taggtaatga gaagaacaga cttaccattg aaatcactcc cagccccgcc
     4021 gttgccagaa atgacatccg ccagccgatg gtttcaccca accaggtcgc cgccggcacg
     4081 ccaccgatat ttgccagggt taaccccata aacatagtgg caactgcgct ggcctgttta
     4141 tgttttggca ccacgcttgc ggccacgact gaacccaaac caaaaaatgc tccgtgattc
     4201 aggctggtca aaatacgtga aagcatcagg gtcatataat ccggcgcgat ggcggaaaga
     4261 acgttgccta gcgtgaaaat tgccatcagg aaaatcaacg cactgcggcg ggcacgatga
     4321 gaaagtagaa gcgtcatcag cggcgcgccc accattacgc caactgcata ggcactgatt
     4381 aacattccgg cagcgggaat cgagacatcc acaccgcgcg caatgacggg caacaagccc
     4441 attggcgaga actccgttgt cccgatacca aacgcgccaa tcgccagcgc cagcaacgga
     4501 tagttaattt tcatgcctta tctccacctc ttcgcgtcat tacgcgatat tcattaaagt
     4561 ggcgaaagca tgacagcaat cacaaaaaaa tgaaaataac aaaaagagaa aacacttttg
     4621 ccattttgct aacaaacagg aaggagatgc gagggagaac gcgctccctc gagaggaaat
     4681 cagtgcagcg cggcagtcaa acccacggct acgatcaaac cgaggacgat aatcgttgtt
     4741 accagtgaaa atttaaggtc ggtgctcatc aagttttctc cttttttatt accacacaaa
     4801 aagtgatatt acgcattttt acacactgtt atgaaaaaat ctcccgtcat ttataatgat
     4861 aagtgttttt accacttccc cttttcgtca agatcggcca aaattccacg cttacactat
     4921 ttgcgtactg gccattgacc ccttcctgac gctccgtgtc gtttttccgg cgtaccgcaa
     4981 cacttttgtt gtgcgtaagg tgtgtaaagg caaacgttta ccttgcgatt ttgcaggagc
     5041 tgaagttagg gtctggagtg aaatggaatg gcaacaataa aagatgtagc gaaacgagca
     5101 aacgtttcca ctacaactgt gtcacacgtg atcaacaaaa cacgtttcgt cgctgaagaa
     5161 acgcgcaacg ccgtgtgggc agcgattaaa gaattacact actcccctag cgcggtggcg
     5221 cgtagcctga aggttaacca caccaagtct atcggtttgc tggcgaccag cagcgaagcg
     5281 gcctattttg ccgagatcat tgaagcagtt gaaaaaaatt gcttccagaa aggttacacc
     5341 ctgattctgg gtaatgcgtg gaacaatctt gagaaacagc gggcttatct gtcgatgatg
     5401 gcgcaaaaac gcgtcgatgg tctgctggtg atgtgttctg agtacccaga gccgttgctg
     5461 gcgatgctgg aagagtatcg ccatatccca atggtggtga tggactgggg tgaagcaaaa
     5521 gctgacttca ccgatgcggt cattgataac gcgttcgaag gcggctacat ggccgggcgt
     5581 tatctgattg aacgcggtca ccgcgaaatc ggcgtcatcc ccggcccgct ggaacgtaac
     5641 accggcgcag gccgccttgc cggttttatg aaggcgatgg aagaagcgat gatcaaggtg
     5701 ccggaaagct ggattgtgca gggtgacttt gaacctgaat ccggttatcg cgccatgcag
     5761 caaatcctgt cgcagccgca tcgccctact gccgtcttct gtggtggcga tatcatggca
     5821 atgggcgcac tttgtgctgc tgatgaaatg ggcctgcgcg tcccgcagga tgtttcgctg
     5881 atcggttatg ataacgtgcg caacgcgcgc tattttacgc cggcgctgac cacgatccat
     5941 cagccaaaag attcgctggg tgaaacagcg ttcaacatgc tgttggatcg tatcgtcaac
     6001 aaacgtgaag aaccgcagtc cattgaagtg catccgcgtt tgattgaacg ccgctccgtg
     6061 gctgacggcc cgttccgcga ctatcgtcgt taatcacccg ttgcgggagt ctcttccggc
     6121 tcccgcagcc actccttatt cagcgtctca ctatcgccga gatactcaag cagccaggtt
     6181 aacgcaggcg acatatcatt ttgctgccat gtcagacaac atgccgaatc cgggaagggg
     6241 ttttccagtt caagtgctac ccacttcccc tcattaagcc acggtttggc gaaatgtgtt
     6301 ggcaccatcc ctatgcataa tcctgccgag atacaggttg ccgatgattc ccagtcaggt
     6361 acgacaagtc ttttttggtt atccagcaac caggtaatac gtttaggtag cgttcgcgag
     6421 gtgtcttcgc gcaccaacga cggccagttg cgcaacgtat catcgctgaa cgggccatcc
     6481 atcaacgcca gcgggtggtg actggcaaca acgcaactcc agcttagcat ccccatatcc
     6541 cggaaggcat aacgaccgcc taccggaatc gcgcgtgttg cgccaattgc cagttccacg
     6601 cgcccgtcgg aaagcgcatc ccagacaccg ttgaacactt cctgaaagac aagaagttca
     6661 acatcatcaa aatggcgata aaaatcaacg atcatctgcc gtgtacgttc tggcctgaca
     6721 atattatcca ctgcgatagc taactgaccg cgccagccgt tcgctatctg ctgacattgc
     6781 tggcgggtga tctgcatttt tttgacaaca gagcgccctt ctttgagaaa ccacgctcca
     6841 gcagcggtca gctccacatc acggtgccgt cgttcaaaga gcggtaccgc cagccactct
     6901 tccagctgac gcacggtata gctgaccgca gaaggaacgc gatgcagctc ctgtgccgca
     6961 gcgctaaaac taccattacg cgctaccgca tcaacaactt cgagtgaata ttctgaccac
     7021 atagtcagcc tgcaaaattt ttgaaaccag tcatcaaata ttaccgtttc acaacactaa
     7081 tttcactccc tacactttgc ggcggtgttt aattgagaga tttagagaat atacatgcaa
     7141 cctgggaaaa gatttttagt ctggctggcg ggtttgagcg tactcggttt tctggcaacc
     7201 gatatgtatc tgcctgcttt cgccgccata caggccgacc tgcaaacgcc tgcgtctgct
     7261 gtcagtgcca gccttagtct gttccttgcc ggttttgccg cagcccagct tctgtggggg
     7321 ccactctccg accgttatgg tcgtaaaccg gtgttattaa tcggcctgac aatttttgcg
     7381 ttaggtagtc tggggatgct gtgggtagaa aacgccgcca cgctgctggt attgcgtttt
     7441 gtacaggctg tgggggtctg cgccgcggcg gttatctggc aagcattagt gacagattat
     7501 tatccttcac agaaagtcaa ccgtattttt gcgaccatca tgccgctggt gggtctgtct
     7561 ccggcactgg ctcccctgtt aggaagctgg ctgctggtcc atttttcctg gcaggcgatt
     7621 ttcgccaccc tgtttgccat taccgtggtg ctgattctgc ctattttctg gctcaaaccc
     7681 acgacgaagg cccgtaacaa tagtcaggat ggtctgacct ttaccgacct gctacgttct
     7741 aaaacctatc gcggcaacgt gctgatatac gcagcctgtt cagccagttt ttttgcatgg
     7801 ctgaccggtt caccgttcat ccttagtgaa atgggctaca gcccggcagt tattggttta
     7861 agttatgtcc cgcaaactat cgcgtttctg attggtggtt atggctgtcg caccgcactg
     7921 cagaaatggc aaggcaagca gttattaccg tggttgctgg tgctgtttgc tgtcagcgtc
     7981 attgcgacct gggctgcggg cttcattagc catgtgtcgc tggtcgaaat cctgatccca
     8041 ttctgtgtga tggcgattgc caatggcgcg atctacccta ttgttgtcgc ccaggcgctg
     8101 cgtcccttcc cacatgcaac cggtcgcgcc gcagcgttgc agaacactct gcaactgggt
     8161 ctgtgcttcc tcgcaagtct ggtagtttcc tggcttatta gtatcagcac gccattgctc
     8221 accaccacca gcgtgatgtt atcaacagta gtgctggtcg cgctgggtta catgatgcaa
     8281 cgttgtgaag aagttgactg cccgaatcat ggcaatgccg aagtcgctca tagcgaatca
     8341 cactgatcta tatcgatata cttatactta ggctgctaac aaaattttgt tgtatgattg
     8401 aaattagcgg cctatactaa tttcgagttg ttaaagctac gataaatatt atgtttttac
     8461 ggggacagga tcgttcccga ctcactatgg atggtcattt cggcaagggt tcctcctttc
     8521 cctctgttct acgtcggatt atagactcgc ggttttttct gcgagatttc tcacaaagcc
     8581 caaaaagcgt ctacgctgtt ttaaggttct gatcaccgac cagtgatgga gaaactatga
     8641 gttcatcgtg tatagaagaa gtcagtgtac cggatgacaa ctggtaccgt atcgccaacg
     8701 aattacttag ccgtgccggt atagccatta acggttctgc cccggcggat attcgtgtga
     8761 aaaaccccga tttttttaaa cgcgttctgc aggaaggctc tttggggtta ggcgaaagtt
     8821 atatggatgg ctggtgggaa tgtgaccgac tggatatgtt ttttagcaaa gtcttacgcg
     8881 caggtctcga gaaccaactc ccccatcatt tcaaagacac gctgcgtatt gccggcgctc
     8941 gtctcttcaa tctgcagagt aaaaaacgtg cctggatagt cggcaaggag cattacgatt
     9001 tgggtaatga cttgttcagc cgcatgcttg atcccttcat gcaatattcc tgcgcttact
     9061 ggaaagatgc cgataatctg gaatctgccc agcaggcgaa gctcaaaatg atttgtgaaa
     9121 aattgcagtt aaaaccaggg atgcgcgtac tggatattgg ctgcggctgg ggcggactgg
     9181 cgcactacat ggcatctaac tatgacgtaa gcgtggtcgg cgtcaccatt tctgccgaac
     9241 agcaaaaaat ggctcaggaa cgctgtaaag gcctggatgt caccattttg ctgcaagatt
     9301 atcgtgacct gaacgaccag tttgatcgta ttgtttctgt ggggatgttc gagcacgtcg
     9361 gaccgaaaaa ttacgatacc tattttgcgg tggtggatcg taatttgaaa ccggaaggta
     9421 tattcctgct ccatactatc ggttcgaaaa aaaccgatct gaatgttgat ccctggatta
     9481 ataaatatat ttttccgaac ggttgcctgc cctctgtacg ccagattgct caatccagcg
     9541 aaccccactt tgtgatggaa gactggcata acttcggtgc tgattacgat actacgttga
     9601 tggcgtggta tgaacgattc ctcgccgcat ggccagaaat tgcggataac tacagtgaac
     9661 gctttaaacg gatgtttacc tattatctga atgcctgcgc aggtgctttc cgcgcccgtg
     9721 atattcagct ctggcaggtg gtgttctcac gcggtgttga aaacggcctt cgagtcgctc
     9781 gctaaaggct attctatcgc cccctcttcg ggggcgagtt cagatcaggc ttctgtacct
     9841 ggttgattca tggcattttc tcgtgccgcc agcacacgtt ctaccgtatc taccactgcc
     9901 tgagtttgtg gatcgatttc aatgttgacg cgtgcgccaa gttttttctt cccaagagtc
     9961 gtgcgttcca gtgtttccgg aattaaatgg acgcaaaaac gcgttggcgt gacttcgccg
    10021 acggtcaggc taataccgtc gatgccaata aatcctttgt acaggatata tttcatcaac
    10081 tgactatcct ggactttaaa ccagatctgg cgattatttt ccgaggttaa tattttcgcc
    10141 acttcagcgg tggtcataat atgacctgac attaagtgtc cgccaatttc atcactgaat
    10201 ttcgccgcac gctcaacgtt tacccaatcc cccactttta aatcgccaag attggtaatg
    10261 cgtaacgttt ctttcatcag gtcaaaactg acatggttgc cgttaatttc cgtcacggtc
    10321 aggcagcaac cgttatgcgc cacggaagca ccggtttcca ggccgtccag catgtggtcg
    10381 ggtaactcca ccacatgcgt acgaaaattt ggtttctcgt caatcgacac cagttttgcg
    10441 gtgccctgta caatccccgt aaacatactt acaactcctg aaatcagtta agacattctg
    10501 ttcagcacaa tagcaggtgg aaaacgccct taccagtgaa ggggtaagaa tggctatttt
    10561 ttcactggag aattaataaa tcctcgctac aatagactga atttcccctg cttcttcttt
    10621 ttgctgccca ttcaggcggc tttttagtct ctcatataac tacaaataaa aggtgttcac
    10681 gtgcagaagt atatcagtga agcgcgtctg ttattagcat tagcaatccc ggtgattctc
    10741 gcgcaaatcg cccaaactgc gatgggtttt gtcgataccg tgatggcggg cggctatagt
    10801 gccaccgaca tggcggcggt cgctatcggt acttctatct ggcttccggc gatcctcttt
    10861 ggtcacggac tgctgctggc attaacgccg gttatcgcgc aattaaatgg ttccggtcga
    10921 cgtgagcgca ttgcgcatca ggtgcgacaa ggtttctggc tggcaggttt tgtttccgtt
    10981 ctcattatgc tggtgctgtg gaatgcaggt tacattatcc gctccatgga aaacatcgat
    11041 ccggctctgg cggacaaagc cgtgggttat ctgcgtgcgt tgttgtgggg cgcgccggga
    11101 tatctgttct tccaggttgc ccgtaaccaa tgtgaaggtc tggcaaaaac caagccgggt
    11161 atggtaatgg gctttatcgg cctgctggtg aacatcccgg tgaactatat ctttatttat
    11221 ggtcatttcg gtatgcctga gctcggtggc gttggttgtg gcgtggctac tgcggcggtg
    11281 tattgggtca tgttccttgc catggtttct tacattaaac gcgcccgctc catgcgcgat
    11341 attcgtaacg aaaaaggcac cgctaagccc gatcctgcgg ttatgaaacg actgattcaa
    11401 ctcggtttgc cgattgcgct ggcactgttc tttgaagtga cactgtttgc cgtcgtggct
    11461 ctgttagtgt ctccgctcgg tattgttgat gtcgcaggac accagattgc cctgaacttt
    11521 agttcactga tgtttgtgct tccaatgtcg ctggcggcag cggtaactat ccgcgtaggt
    11581 tatcgtctgg gtcagggctc aacgctggat gcgcaaaccg ctgcgcggac cgggcttatg
    11641 gtgggtgtct gtatggcaac cctgacggcc attttcacgg tttcactgcg ggagcaaatc
    11701 gccctgttgt acaacgacaa tcccgaggtt gtaacgctgg ctgcgcattt gatgttgctg
    11761 gcggcggtat atcagatttc tgactcaatc caggtgattg gcagtgggat tttgcgtggt
    11821 tataaagata cgcgttccat tttctatatc acctttacag cttactgggt gctgggcttg
    11881 ccaagcggct atattctggc actgaccgat ctggtcgttg aacctatggg gccagcaggc
    11941 ttctggatag gctttattat cggcctgaca tcggcagcca ttatgatgat gctgcgtatg
    12001 cggttcctgc aacgtatgcc gtcagccatc attctgcaac gagcgtcccg ctaataaaga
    12061 caaggcgcaa ccttcacggg ttgcgcctgt gtttttacgc aggctggagc gttgcgccaa
    12121 tcccgtcttc gtctggctgt aatttcagag cgttacacag agtttgccga ctggcatctg
    12181 ttatctcaac gcgtatatcc ccgttaactt cccctttcac attgatgaag tcaggtgaaa
    12241 tttctttcgc actggttttc aggcaaatcg ctccctctcc gcgcaggcgt ttcactttca
    12301 gttgcacatg cgttgccgca ccagtgatca gccacagttt tccgccattc agattgatgt
    12361 taccaacaat gaaagtgacg ctgtcacctt ttaccgtact gccgtcgaga atcaacacac
    12421 cgccctgctg tacagtaatc gttgattgtt gcagctctgc gccataacca ataatcattg
    12481 aagcgccgtc cagtaatgca acactcccgg tcagttcaac cggtgcgcga tcttcctcta
    12541 cgacaaaatt ttcgtccgcg ctggtgggtg acaacaggat cactgttcca ccttgtatta
    12601 ccgcattttc cagcgtaccg gcatgcactt cagcccgatc ccccacattc accgacaggt
    12661 tttgcgtctt accggaactg tagagctgaa ggccatccgt ccccagacga taaatggatc
    12721 cattctcaac aatagtgtca gagtcttcac caccgagatt attcatggtg ccaccagcaa
    12781 gcaacgtatc actggctttc ccggccagca taaaaacgcc gccctgatta atctcacaat
    12841 tgctggcttc tccacttgga tacaccaaca attcaccacc ttcatctacc acgaccgctg
    12901 aggttgtccc attaaccaac aggccgccct cttgatcgag aatgattttt tccgcgcgat
    12961 gtccttccag tacacgcagg ttaccgccat tttccagcaa caaaccgtag gcataaccct
    13021 gatcaacgct gaattcacct tcgggatggc ggccattaac cgtagcgagc gtagagagac
    13081 taattgcgcc tccctcggac tgaacaatat ctgtggcaat ggcaccgtcg ctgaccgata
    13141 gcgtgccatt gttaccgtac tgtgtgcatt tggcgatccc accggttgcc actttcaacc
    13201 agccagcccc cgtgaccacg gtattttcgg ctcttccgcc agcttcaaca atcaactcgc
    13261 cttcagtaag ttgcgtcgcg ccaatgacct cgccggtctt aacaatttgc acattcccgt
    13321 cgctcatcaa gtttttcgca tcagatccca tgatttattc ctttgctgca tctgtgtgcc
    13381 tttattgcta cctaagtgta aaggctacgg aggatttatc cacgacagat ttgagatggt
    13441 ggcaaacaac tctgtttaaa ctctgataca cgaattattg ggttgtatca gatgtaaatg
    13501 cgatcctgaa taaaaatcgc ccttgcaaat caacaaaata tagccaattt gcttaaaagc
    13561 tctgcaaacg gtcgaaatag cgcagaaaat tacgttttgc ctcttgccac ctttccactc
    13621 tgccgctaat attcgtcccc gttgtcacct acaacgttgc gttcatagct cagttggtta
    13681 gagcaccacc ttgacatggt gggggtcgtt ggttcgagtc caattgaacg caccatcctg
    13741 cgtccgtagc tcagttggtt agagcaccac cttgacatgg tgggggtcgg tggttcgagt
    13801 ccactcggac gcaccagatt ttcttaatct ggtcttttcc tttttccctc tgtttcttct
    13861 ctgtatccaa tacgttaaaa gatttacact gtcttcgtat gcgttatcag aaggagaatc
    13921 gctatggcaa ctttgttaca atttcatttt gcttttaatg gcccatttgg cgacgcaatg
    13981 gctgagcagc ttaaaccact tgctgagtcg attaatcagg aacctggttt tctgtggaag
    14041 gtatggacag aaagtgaaaa gaaccacgaa gccggtggga tctacctttt cactgatgaa
    14101 aaaagcgccc ttgcctatct ggaaaaacat actgcccgac tgaaaaatct cggcgttgag
    14161 gaagttgtcg ccaaagtttt cgatgtcaat gagccactta gtcaaatcaa tcaggcaaaa
    14221 ctcgcctgac agaatttaat caagggcggt tagcgccctt ttcatccctg tctgaaattt
    14281 ctcaaattct aaaaatctca accaaactta tctgataaca ctaaattcga aagaatgcgt
    14341 acaggtaagt aacaatgaaa aaaattgcta ttgtgggtgc cgggcctacg gggatctaca
    14401 ccttattctc gcttctacag caacaaactc cactttctat ttctatcttc gagcaggctg
    14461 acgaggccgg tgtcgggatg ccatacagtg atgaggaaaa ctcaaaaatg atgctggcaa
    14521 atattgccag tattgaaata ccgccgatta attgtacgta tctcgaatgg ctacaaaagc
    14581 aagaagccag ccatctccag cgttatggcg ttaaaaaaga aaccttgcac gatcgtcagt
    14641 ttttaccgcg aattctgctg ggcgaatatt tccgcgatca atttttacga ttagtagacc
    14701 aggcacgaca gcaaaaattt gcagtggctg tttatgaatc atgccaggtt accgatctgc
    14761 aaattacaaa tgctggcgtc atgctcgcta caaatcagga tttacccagc gagacgtttg
    14821 atttagcggt gattgccacg gatca
//
LOCUS       DADLTF010000102        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.102, whole genome shotgun sequence.
ACCESSION   DADLTF010000102 DADLTF010000000
VERSION     DADLTF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.102"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            116..1417
                     /gene="rstB"
                     /locus_tag="JN749_002873"
     CDS             116..1417
                     /gene="rstB"
                     /locus_tag="JN749_002873"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HAY7369228.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1493..2422
                     /gene="tus"
                     /locus_tag="JN749_002874"
     CDS             1493..2422
                     /gene="tus"
                     /locus_tag="JN749_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HAY7369229.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JN749_002875"
     CDS             complement(2419..3822)
                     /gene="fumC"
                     /locus_tag="JN749_002875"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HAY7369230.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3965..5611)
                     /locus_tag="JN749_002876"
     CDS             complement(3965..5611)
                     /locus_tag="JN749_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HAY7369231.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5810..6985
                     /gene="manA"
                     /locus_tag="JN749_002877"
     CDS             5810..6985
                     /gene="manA"
                     /locus_tag="JN749_002877"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAY7369232.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLRDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANKSPVTVKGHGRLARVYNKL"
     gene            7086..8594
                     /locus_tag="JN749_002878"
     CDS             7086..8594
                     /locus_tag="JN749_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HAY7369233.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JN749_002879"
     CDS             complement(8820..10085)
                     /gene="uidC"
                     /locus_tag="JN749_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HAY7369234.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JN749_002880"
                     /pseudo
     CDS             complement(10124..11497)
                     /gene="uidB"
                     /locus_tag="JN749_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JN749_002881"
     CDS             complement(11494..13305)
                     /gene="uidA"
                     /locus_tag="JN749_002881"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAY7369235.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13693..14283)
                     /locus_tag="JN749_002882"
     CDS             complement(13693..14283)
                     /locus_tag="JN749_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7369236.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14512..>14685)
                     /locus_tag="JN749_002883"
     CDS             complement(14512..>14685)
                     /locus_tag="JN749_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7369237.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3685 a         3820 c         3495 g         3685 t
ORIGIN      
        1 atacatccgt ctgttccgga cattatcttc caaacaaggt cttatccagt tggcggttta
       61 cattgctcca cagtcgtgaa ttaatacgaa tagtattctg ttgagggcga tgccaatgaa
      121 aaaattgttt atccagtttt acctgttatt gtttgtctgc ttccttgtga tgtctctgct
      181 ggttgggctg gtgtacaaat ttaccgccga acgcgcgggc aaacagtcgc tggatgattt
      241 gatgaacagt tcgctgtatc tgatgcgcag cgaattgcga gagatccccc cacacgactg
      301 gggtaagacg ctgaaagaga tggatttaaa tctctctttc gatctgcgtg tcgagccact
      361 gagtaaatac catcttgatg atatttccat gcaccgactg cgtggcggcg aaattgtcgc
      421 cctggacgat cagtacacgt ttttgcagcg tatcccgcgc agccactacg tgctggcagt
      481 tggtcctgtt ccttatcttt attacctcca tcaaatgcga ttgctggata tcgccctgat
      541 cgcttttatt gctatttccc tcgcctttcc ggtgtttatc tggatgcgtc cgcactggca
      601 ggatatgtta aaactggaag cagcggcgca acgatttggc gatgggcatc tcaatgaacg
      661 tatccacttt gatgagggtt cgagctttga acgacttggt atcgcattta accagatggc
      721 ggacaatatc aacgccttaa ttgccagcaa aaaacagctt attgacggta tcgctcacga
      781 actgcgaaca ccgttagtgc gcctgcgtta tcgactggag atgagcgata acctgagcgc
      841 cgccgaatcc caggcgttga atcgtgatat cagtcaactt gaagctttaa ttgaagagct
      901 gctgacttat gcccgactcg atcgcccaca aaacgagctt catcttagcg aaccagacct
      961 gccgttgtgg ctgtcaacgc atctggcaga tattcaggca gtaacgcccg ataaaacggt
     1021 acggataaaa acgctcatgc aaggccatta tgcggcgttg gatatgcgct taatggagcg
     1081 cgtgctggat aatttgctca ataacgccct gcgctactgc cattcaacgg ttgaaaccag
     1141 cctgctactg tcggggaata gagcgacatt aattgttgag gatgatggcc cagggattgc
     1201 cccagaaaac cgcgaacata tctttgaacc ttttgttcgc ctcgatccca gccgggatcg
     1261 ctcaaccggc ggctgcgggc tggggctggc aattgtccac tctatagcac tggcaatggg
     1321 cggtacggtt aattgtgaca tcagcgaact gggtggtgct cgcttctcgt ttagctggcc
     1381 gttatggcat aacatcccgc aatttacctc tgcctgacac tacgcgcacg atggtcaagt
     1441 cacaacgact gtgctataaa ataagtatgt tgtaactaaa gtggttaata ttatggcgcg
     1501 ttacgatctc gtagaccgac tcaacactac ctttcgccag atggaacaag agctggctac
     1561 atttgccgct catcttgagc aacacaagct attggttgcc cgcgtgttct ctttgccgga
     1621 ggtaaaaaaa gaggatgagc ataatccgct taatcgtatt gaggtaaaac aacatctcgg
     1681 caacgacgcg cagtcgctgg cgttgcgtca tttccgccat ttatttattc aacaacagtc
     1741 cgaaaatcgc agcagcaagg ccgctgtccg tctgcctggc gtgttgtgtt accaggtcga
     1801 taacctttcg caagcagcgt tggtcagtca tattcagcac atcaataaac tcaagaccac
     1861 gttcgagcat atcgtcacgg ttgaatcaga actccccacc gcggcacgtt ttgaatgggt
     1921 gcatcgtcat ttgccggggc tgatcaccct taatgcttac cgcacgctca ccgttctgca
     1981 cgaccccgcc actttacgct ttggttgggc taataaacat atcattaaga atttgcatcg
     2041 tgatgaagtc ctggcacagc tggaaaaaag cctgaaatca ccacgtagtg tcgcaccgtg
     2101 gacgcgcgag gagtggcaaa gaaaactgga gcgagagtat caggatatcg ctgccctgcc
     2161 acagaacgcg aagttaaaaa tcaaacgtcc agtgaaggtg cagccgattg cccgcgtctg
     2221 gtacaaagga gatcaaaaac aagtccaaca cgcctgccct acaccactga ttgcactgat
     2281 taatcgggat aatggcgcgg gcgtgccgga cgttggtgag ttgttaaatt acgatgccga
     2341 caatgtgcag caccgttata aacctcaagc gcagccgctt cgtttgatca ttccacggct
     2401 gcacctgtat gttgcagatt aacgcccggc ttttatactg ccgaccatct gttctggccg
     2461 tacccagctg tcaaactcgg cttcgctaag ataccccagc gcaagggccg cagcttttaa
     2521 ggtcagccct tctttatgcg cttttttggc gatctccgcg gctttgtcat aaccaatgtg
     2581 ggtgttaagc gcagtcacca gcatcagcga ttcattgagt aattgattga ttcgctcacg
     2641 attcggttca atacccactg cgcagtgttt gttaaaactt tccatgccat ctgccagcaa
     2701 gcgcaccgat tgcaggaaat tatggatcac catcggacgg aagacgttca gttcaaagtt
     2761 accggaagcg ccacccatgt tgatcgccac gtcgttcccc attacctgac agcagagcat
     2821 ggttaatgct tcacactggg ttgggttcac tttccccggc atgattgagc tgcccggctc
     2881 attttccggg attgagattt caccaattcc gcagcgcggg ccagaggcca gccagcggac
     2941 atcattggcg attttcatca gtgacgcagc caaccctttc aacgcgccgt gcgcctgaac
     3001 cagggcatca caggtcgcaa gcgcttcaaa tttgttcggc gcggtaacaa acggtgcaca
     3061 ggtaatgact gccagttcat ctgctacgcg ccgcgcatac tccggatggg tatttagtcc
     3121 agtacccacc gctgtaccgc caagagccag ttccgctacg tgaggcaggc tgtattcgat
     3181 atgtttgaga ttatgctcga gcatcgctac ccagccggaa atctcctgcc ctagtgttag
     3241 cggcgtggcg tcctgcaagt gggttcgacc gattttgacg atatcggcaa atgcacgcga
     3301 tttttcactc agcgtctgtg tcagggtttt aagctgcgga atgagttgct tgcgcagtgc
     3361 cagcagcgcc gcaacgtgca tcgccgtcgg aaagacatcg ttggaacttt ggcttttgtt
     3421 cacgtcgtcg ttagggtgaa ctttacgttc catcccgcgc acaccgccga gtaattcact
     3481 ggcccggtta gccagcactt cgttcatgtt catgttactt tgcgtgccgg agccggtctg
     3541 ccagatagcc agcgggaatt cgtcgtcatg ctgtcctgcc agtacttcat ccgccgcctg
     3601 ccgaatggcg ctcgctttct cttcagacaa caagtctaaa tcttcattaa cttttgccgc
     3661 tgcgcgcttg gttagcgcca gcgcatgaat cagtgaggtg ggcattttct ccgtcgaaat
     3721 gcggaaatgc tccagcgagc gttgagtttg tgcgccccac agcttatctg ccgggacatc
     3781 aatcgccccc atcgaatctt tttcgctgcg tactgtattc atggcctgct cctcacctga
     3841 ttaatttttt ctttctgttt tgctttcgtt aagcaacttt tagctcactc attatttact
     3901 atttgataac aaatgtttgg tctttcgtgc catgtaaaaa aaccgccccg aagggcggct
     3961 ctgtttattt cacacagcga gtgcactgtg tgagttgtat ctgctggaag aagtcatttc
     4021 ctttatcatc cacaaggata aacgccggga aatcttccac ttcaattttc cagatggctt
     4081 ccattcccag ttccggatat tcaacacatt ccaggctctt aatacttccc tgcgccaata
     4141 cagcggccgg accaccgata ctgccaaggt agaagccgcc gtgtttttta caggcatccg
     4201 tcacctgctg gctgcggttg cctttcgcca gcatgatcat acttccgccc tgcgcttgca
     4261 gttgatcgac ataagaatcc atccgtccgg cggtcgttgg gccaagagaa ccggaggcat
     4321 aaccttccgg cgttttggcc ggacccgcgt agtaaatcgg atgatcttta atgtattgcg
     4381 gcagcccttc accgttatcc atccgctctt tcagtttggc gtgagcaata tcacgaccaa
     4441 cgataatcgt gccgttaagc gataagcgtg tagaaacggg atactgcgac aactgtgcga
     4501 ggatctcttt catcggacgg ttaaggtcaa cgcgcaccgc ttcgccttct cccgctttac
     4561 gcagctcttc agggatatat ttacctggat tatgttccag tttttcgatc cagatcccct
     4621 gacggttgat cttcgctttg atattacggt cagcagagca ggaaacaccc ataccaaccg
     4681 gacaggatgc gccgtgacgt ggcaggcgaa tcacgcggat gtcgtgagcg aagtatttac
     4741 caccaaactg cgcacccaga ccgagatttt gcgcttcgat cagtaattct ttttccagtt
     4801 ccacatcgcg gaacgcctga ccgtgctcat tcccttccgt tggcagttca tcatagtatt
     4861 tcgcggaagc cagtttcacc gttttaaggt tcgtttctgc agaagttcca ccaataacga
     4921 acgcaatatg atacggagga caggccgcgg tacccagcgt gcgcatcttc tcaaccaggt
     4981 aatttttcag tttccccggc gtcagtaacg ctttggtttc ctgatagaga tacgtcttgt
     5041 ttgccgaacc accacctttt gcgatacaga ggaatttgta ttcgtcgcca tcaacggcat
     5101 aaagatcgat ctgcgctggc agattggtgc cggtattcac ttctttatac atatccagcg
     5161 gcgcgttttg cgagtagcgc agattatctt cgatataagt gttatagaca ccgcgcgcca
     5221 gcgccgcttc atcaccacca ccggtccata cacgctgccc ttttttacca acaataatcg
     5281 cggtgccggt atcctgacag gttggcagaa cgcctttcgc cgcgatgtcg gagttacgca
     5341 ggaattgcag cgccacatat ttatcatttt cgctggcctc cgggtcacgc agaatgtcgg
     5401 ccacctgttg ttggtgagcc ggacgcagca taaacgacgc atcatgaaac gcctgacgcg
     5461 ccaacagagt taacgcttcg ggtgcgactt tcaaaatctc ctgcccttca aattcagata
     5521 cgctaacgtg ttcactggtt agcaggtaat actcagtatc atcttttttg agtggaaaag
     5581 gagactgata atgaaagggt ttgtttgaca ttgttctctc acttactgcc tggtttggtt
     5641 atgctctggg cgggtgttcc gttgccctgt taaaagcgag taacaatatc ctacacagtt
     5701 ttttaacaaa aactgagact agtacgactt tttgcggctc caggttactt cccgtaggat
     5761 tcttgcttta atagcgggat taatttccac attaaaacag ggattgatca tgcaaaaact
     5821 cattaactca gtgcaaaact atgcctgggg tagcaaaacg gcgttgactg aactttatgg
     5881 tatggaaaat ccgtccagcc agccgatggc cgagctgtgg atgggcgcac atccgaaaag
     5941 cagttcacga gtgcagaatg ccgccggaga tatcgtttca ctgcgtgatg tgattgagag
     6001 tgataaatcg actctgctcg gagaggccgt tgccaaacgc tttggcgaac tgcctttcct
     6061 gttcaaagta ttatgcgcag cacagccact ctccattcag gttcatccaa acaaacacaa
     6121 ttctgaaatc ggttttgcca aagaaaatgc cgcaggtatc ccgatggatg ccgccgagcg
     6181 taactataaa gatcctaacc acaagccgga gctggttttt gcgctgacgc ctttccttgc
     6241 gatgaacgcg tttcgtgaat tttccgagat tgtctcccta ctccagccgg tcgccggtgc
     6301 acatccggcg attgctcact ttttacaaca gccacatgcc gaacgtttaa gcgaactgtt
     6361 cgccaacctg ttgaatatgc agggtgaaga aaaatcccgc gcgctggcga ttttaaaatc
     6421 ggcgctcgac agtcagcagg gtgaaccgtg gcaaacgatt cgtttaattt ctgaatttta
     6481 cccggaagac agcggcctgt tctccccgct gttactgaat gtggtgaaat tgaatcctgg
     6541 cgaagcgatg ttcctgttcg ctgaaacacc gcacgcttac ctgcaaggcg tggcgctgga
     6601 agtgatggcg aactccgata acgtgctgcg tgcaggtctg acgcctaaat acattgatat
     6661 tccggaactg gttgccaatg tgaaattcga agccaaaccg gctaaccagt tgttgaccca
     6721 gccggtgaaa caaggtgcag aactgggctt cccgattcct gtggatgatt ttgccttctc
     6781 gctgcatgac cttagggata aagaaaccac catcagccag cagagtgccg ccattttgtt
     6841 ctgcgtcgaa ggcgatgcaa cgttgtggaa aggttctcag cagttacagc ttaaaccggg
     6901 tgaatcagcg tttattgccg ccaacaaatc accggtgact gtcaaaggcc acggccgttt
     6961 agcgcgtgtt tacaacaagc tgtaagagct tactgaaaaa attaacatct cttgctaagc
     7021 ttattaaagg cttataacac cttcaggcgg ccagtccgcc tgatttcatt ttatggataa
     7081 tcattatgaa taaatcgctg gtagcggtag gcgtcattgt tgcgctaggc gtagtctgga
     7141 caggcggcgc atggtataca ggcaagaaga ttgaaaccca tctcgaagac atggtcgcgc
     7201 aggcgaacgc gcaactcaaa ctgacagctc ctgaatccaa cctggaagtg agttatcaaa
     7261 actatcatcg cggcgtattc agcagccagt tgcaactgtt ggtgaaaccc attgctggga
     7321 aagaaaatcc gtggattaaa agcggtcaga gcgtcatctt caacgaatcg gttgatcatg
     7381 gtcccttccc gcttgcccag cttaaaaaac tgaacctgat cccgtcgatg gcatcaattc
     7441 aaaccacgct ggttaataac gaagtaagca aaccactgtt tgatatggca aaaggtgaaa
     7501 cgccttttga gattaactcg cgcattggtt acagcggtga ttccagttcc gatatttcgc
     7561 tcaatccact gaattacgag caaaaggatg aaaaagtcgc ctttagcggc ggcgagttcc
     7621 agttaaatgc tgacagagac ggcaaagcca tctccctttc cggggaggcg caaagtggtc
     7681 ggatagacgc agttaacgaa tacaaccaga aagtgcagtt gacctttaat aatctgaaaa
     7741 ccgacggttc cagcacgctg ggaagttttg gtgagcgcgt aggaaaccaa aaactgtcac
     7801 tggaaaaaat gaccatttca gtggaaggca aagaactggc actgctggaa ggcatggaga
     7861 tcagcggtaa atcggatctg gtcaatgacg gtaaaacgat caatagccaa ctggattact
     7921 cgctaaacag cctgaaggta cagaatcagg atctgggcag cggcaagctg actttaaaag
     7981 tcggccagat tgatggtgaa gcctggcatc agtttagcca gcaatataac gcgcaaactc
     8041 aggcgctgct ggcacagcca gaaattgcca acaatcccga actttatcag gagaaagtga
     8101 cggaagcctt ctttagcgcc ctgccgctga tgttgaaagg cgatccggtg attactatcg
     8161 cgccgctaag ctggaaaaac agtcagggtg aaagtgcgct gaatctgtcg ctgttcctga
     8221 aagatccggc aacgactaaa gaagcgccgc aaacgctggc gcaggaagta gatcgttcgg
     8281 ttaaatctct ggatgcgaaa ctgaccattc cggtggatat ggcaactgag ttgatgactc
     8341 aggtagcgaa gctggaaggt tatcaggaag atcaagcgaa aaaactggcg aaacagcaag
     8401 ttgaaggtgc atcagcaatg gggcagatgt tccgtctgac caccttgcag gacaatacca
     8461 tcaccaccag cctgcaatat gctaacggtc agataacgtt aaatgggcag aaaatgtcgt
     8521 tagaagattt tgttggcatg tttgcgatgc cagctcttaa cgttccggct gtaccggcaa
     8581 ttccgcagca gtaattcacg gccaaaacgc cggagaattt tcactccggc gttttcatct
     8641 gaaaacaaac cagagattgc ctgatgcgct acgcttatca ggcctacctt ttcctgcaac
     8701 actttgaatt tatgagtttt tgtaggctgg ataaggcgtt tacgccgcat ccggcataaa
     8761 aaacgcgcac tttgtcaaca atctgaaacg ccggaggttt ttctctccgg cgttatttct
     8821 tacttcagca taaagtcata ctgaatactc attttcacat cccagtaatt caggcgatcg
     8881 cccagcgtac tgccatcgcg cttttcccgc cctttatcgg cagcaagaaa gaaattcatc
     8941 ccttgtaacg gcccttcgct gacccgatag ttcataccac ctaagatacc cgcgccttca
     9001 atccgcacat ctcgtttact ggattcatag ccgcgagtca caatcggtgc aaatgtcagc
     9061 gtaaattgcg gagttacacg gtagttgaca cctaattgcc aggactgcat tccttcacgg
     9121 ttacgatcaa gacttaatga aaagggatag ccaatgtcgg tatccaccac ggcaccattc
     9181 attgcatggc gcaaataacc accactgcca aatacgcttc cccacggcgc attccaggtc
     9241 aattgtccac taaccaacgc cgtttcattg ggctggctgg tattacgact taaaccttcg
     9301 agctgcgcgt aaaagccgag caactcccct ttcaacgtat aattcgggtc gagcggtatg
     9361 cttttctcga gaatactttg tatctgattg gaaacatctt tctgtaccga tgcccccaga
     9421 gaatagcgac tggtatcatc atgccaactg gcggctaaaa aagaacgtgc gcgatgttta
     9481 tcgtattttc ccgtctggct gttatacacc ccttcatcac gaataccggt tgttgaatca
     9541 gtccataaaa atactcgtga acgccacatt ccccacagat caatatttgg tgttaaagcc
     9601 caataaccta atgctccttc agaaactgtc ggtaatgtac gttgcgaact gccagccatt
     9661 aacggaatac gataaggtaa gctaccataa ctataatccg tcccgaagcg acctaattta
     9721 agcaggctat tttcactggg ttttattttt actgccccca gggcaaagcc aaaactttta
     9781 tcaccatcaa gataccaccg ggtactcata tcagcacgag cacctaattt ataaacataa
     9841 tacgctccgc cttcaacgcc aataatattg gaataataac cactattgaa atcgagcaat
     9901 cctccctgta cccatgcgta aatatctctt cccgcgccag cactgggctt atcagccctg
     9961 cgcaattcgt ttttcagtct gatgcgtagt cccgcttcat cgtcagccag ttgcgccacg
    10021 taagcagagg taaggccaga ggcagccgtc aggcaaataa cggccatggc cactattttt
    10081 ctcatagttg cacctttgat gttctgatta tttttattga atattaatta gtgatatcgc
    10141 tgattaattg ctgctgcact tttttacgat tatcaatttc aaccacgatt tctttgaatt
    10201 ttttatccgt gagcggataa aaccagataa taacgaatgc cagtagcata aatccgcaag
    10261 gtactaaggc aattgatgtg cggatgccca taataacttc cggcgtttac acctgattgg
    10321 cgatatatcc gcttaatccc aaaataaagg caggaattga acctccgatt gcctgaccgc
    10381 atttacgggt aaatgagaat agtgaatagg tgagcccttc aattcgcacg ccggtcaggt
    10441 attcaccgta ttctacggta tcagcttcca gtgcccacat cacagtcata gtaacgccct
    10501 gaccaattga agcgatggcc aacgcaacca acgccaccgg cagtgaccag acggaaaccc
    10561 agaagaatag cagataaccg caggttccca gcaaagcgcc aatcaggaag gtattctttt
    10621 taccgatcct cgcgaccatc cccggcacaa gcggtgccga tgccacagta ccaaccaggt
    10681 tttgcaccag taccagcaca gtgaacagcc cggtatcatt taacacatag cgcacgtaga
    10741 acaacgacga ggcgctgacc gcgaaggtcg aaatcagcac acacagcgca ccgatgcaca
    10801 acataaacag cgggcgattc cgtttcaggg tttgcaaact gatcttcaat gacggctgcg
    10861 ccacgatacg taccacattt tcacgcgtcg atttgaagca gatgaagtaa agcaccattc
    10921 cggcaatcgc cagcacaatc gtccagaaat ggtataccga caccatctct tccgggctgg
    10981 agttcttaat gctcggtcct atcagaaatg ccaggcatac aaaggtcaat gaagcggcaa
    11041 tcccacgagc cgcgcccaga cgggcgcgag attgtggttg ttgggtcatc gcggtagcaa
    11101 gtgaaccata aggaatattc accaggctgt agcaaagccc gaggcccatg taggtcaaat
    11161 atgcatacac cactttgcta ccatggctcc agtcggtcgg tacccagaat accagcacgc
    11221 tgaagatcat taacggcgca gtaccgaaga gtaaaaacgg gcggaatttt ccccagcggg
    11281 tattcacact gtccaccact cgtccggcaa agacgtcggc gaaggcatca aatacccgca
    11341 ccagtaacag catggtgcct gccgcagcgg caccgacgcc agcgacgtcg gtgtagtaac
    11401 tcaataggaa gagcgccccc attgcgaagg cgaagttatt ggcgacgtca ccgaggctgt
    11461 agccgacgat ggtgcgccag gagagttgtt gattcattgt ttgcctccct gctgcggttt
    11521 ttcaccgaag ttcatgccag tccagcgttt ttgcagcaga aaagccgccg acttcggttt
    11581 gcggtcgcga gtgaagatcc ctttcttgtt accgccaacg cgcaatatgc cttgcgaggt
    11641 cgcaaaatcg gcgaaattcc atacctgttc accgacgacg gcgctgacgc gatcaaagac
    11701 gcggtgatac atatccagcc atgcacactg atactcttca ctccacatgt cggtgtacat
    11761 tgagtgcagg ccggctaacg tatccacgcc gtattcggtg atgataatcg gctgatgcag
    11821 tttctcctgc caggccagca gttctttttc cagtaccttc tctgccgttt ccaaatcgcc
    11881 gctttggaca taccaaccgt aataacggtt caggcacagc acatcaaaga gatcgctgat
    11941 ggtatcggtg tgagcgtcgc agaacattac attgacgcag gtgatcggac gcgtcgggtc
    12001 gagtttacgc gttgcttccg ccagtggcgc gaaatattcc cgtgcacctt gcggacgggt
    12061 atccggttcg ttggcaatac tccacatcac cacgcttggg tggtttttgt cacgcgctat
    12121 cagctcttta atcgcctgta agtgcgcctg ctgagtttcc ccgttgacag cctcttcgct
    12181 gtacagttct ttcggcttgt tgcccgcttc gaaaccaatg cctaaagaga ggttaaagcc
    12241 gacagctgca gtttcatcaa tcaccacgat gccatgttca tctgcccagt cgagcatctc
    12301 ttcagcgtaa gggtaatgcg aggtacggta ggagttggcc ccaatccagt ccattaatgc
    12361 gtgatcgtgc accatcagca cgttatcgaa tcctttgcct cgcaaatccg catcttcatg
    12421 acgaccaaag ccagtaaagt agaacggttt gtggttaatc aggaactgtt cgcccttcac
    12481 tgccactgac cggatgccga cgcgcagcag gtagatatca cactctgtct ggcttttggc
    12541 tgtgacgcac agttcataga gataaccttc acccggttgc cagaggtgcg gattcaccac
    12601 ttgcaaagtc ccgctggtgc cttgtccagt tgcaaccacc tgttgatccg catcacgcag
    12661 ttcaacgctg atatcaccat tggccaccac ctgccagtca acagacgcgt ggttacagtc
    12721 ttgcgcgaca tgcgtcacca cggtgatatc gtccacccag gtgttcggcg tggtgtagag
    12781 cattacgctg cgatggatcc cggcatagtt aaagaaacca tggaagtaag actgcttttt
    12841 cttgccgttt tcgtcggtaa tcaccattcc cggcgggata gtctgccagt tcagttcgtt
    12901 gttcacacaa acggtgatac gtacactttt cccggcaata acatacggcg tgacatcggc
    12961 ttcaaatggc gtatagccgc cctgatgctc catcacttcc tgattattga cccacacttt
    13021 gccgtaatga gtgaccgcat cgaaacgcag cacgatacgc tggcctgccc aacctttcgg
    13081 tataaagact tcgcgctgat accagacgtt gcccacataa ttacgaatat ctgcatcggc
    13141 gaactgatcg ttaaaactgc ctggcactgc aattgcccgg ctttcttgta acgcgctttc
    13201 ccaccaacgc tgatcaattc cacagttttc gcgatccaga ctgaatgccc acaggccgtc
    13261 gagttttttg atttcacggg ttggggtttc tacaggacgt aacataaggg actcctcatt
    13321 aagataaaaa tactggtcaa cctttaatct gattagttaa ccaattctgt gttgcacaca
    13381 gttatctgtg agaaacccat ggaagagaaa tggaaataaa aagaactgac accgcgatca
    13441 aaaaaccaaa aactgcaata cccacagcgt gatatggatc gcattaatct ttaaaacagc
    13501 gttgacagaa gccataaaac aagcaatcga gcgttcgttc gagagtattg ggaacgataa
    13561 actctcatga catatggcta cggtgaatat tttggggggg aattctccga acacggggag
    13621 gataattttt gcacagagaa attacgatat gaaacgcggg cacagttatc accagcccgc
    13681 gaaatgagag gatcaggatg cggttaatat accgccaatc atcgcgcgta atccctgagc
    13741 aaacgacgct tcacgcgcca ggtcttccgc ttcgatatcg gccagtacac cgtaggtcag
    13801 atccagcagt aaacgtgaag tcattgccgt gttgatgtct gggcttatct cgcctttttg
    13861 ctgggcatca cgcatccgct gggcaacaaa ttcagtgatc gtcatatgct tatttttaag
    13921 catggcggcg acctgtgggt tacgcatccc ttccgccata atttcgacca ccagcgcccg
    13981 ttgcccaaag gcttcatggg tgagagagac aatagactcg accatatagt caacaaaatg
    14041 aatcccttca atcggttccc ggaaacgagc cagcgccctc tcctggtcct gtaagataat
    14101 cgcctgaatc aaggcttctt tggagatgaa atggtgatag agcgtcccag gactaatggc
    14161 gcaagattta cagatggctt tcatcgaggc actgtgaaat ccattttctg aaaaaatctc
    14221 tctggcagca ttgaggatcc gggtccgtgt cggttgtgct tcagtctgca tgttatccat
    14281 cattaaacca ttatagttag cgtgaataac tagtttaccg cgttgtcagg tgagcgccta
    14341 tcattgctgt tgaatagtgc gaaggtacac tctattcgta tacttttcac ccagcaattg
    14401 gttaaccaat tgccgtgatt tggctgccag ttgtttagca gagacgttga tgaattgcac
    14461 acgctatccc ggtaaacaac acatacgacc gtttaccggt tcgttagtgt attaattgag
    14521 ctcctgtacc ccaccaccgg agacggtgag aatttgtccg cttacccagc tggctgcagg
    14581 cgagcaaagg aacagcgccg cgttagcaat atcttgcggt tggcccagac gtctgatcgg
    14641 cgtgtgctgt aacatttttt gttcaatttc tgggtaatga ctcca
//
LOCUS       DADLTF010000103        14685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.103, whole genome shotgun sequence.
ACCESSION   DADLTF010000103 DADLTF010000000
VERSION     DADLTF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.103"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            156..1196
                     /locus_tag="JN749_002884"
     CDS             156..1196
                     /locus_tag="JN749_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HAY7369238.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1229..2422)
                     /gene="lplT"
                     /locus_tag="JN749_002885"
     CDS             complement(1229..2422)
                     /gene="lplT"
                     /locus_tag="JN749_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HAY7369239.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2415..4574)
                     /gene="aas"
                     /locus_tag="JN749_002886"
     CDS             complement(2415..4574)
                     /gene="aas"
                     /locus_tag="JN749_002886"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HAY7369240.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            4963..5994
                     /gene="galR"
                     /locus_tag="JN749_002887"
     CDS             4963..5994
                     /gene="galR"
                     /locus_tag="JN749_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HAY7369241.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6001..7263)
                     /gene="lysA"
                     /locus_tag="JN749_002888"
     CDS             complement(6001..7263)
                     /gene="lysA"
                     /locus_tag="JN749_002888"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAY7369242.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7385..8320
                     /gene="lysR"
                     /locus_tag="JN749_002889"
     CDS             7385..8320
                     /gene="lysR"
                     /locus_tag="JN749_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HAY7369243.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8307..8999)
                     /gene="ygeA"
                     /locus_tag="JN749_002890"
     CDS             complement(8307..8999)
                     /gene="ygeA"
                     /locus_tag="JN749_002890"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HAY7369244.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAS
                     IHAEDAVAFMLS"
     gene            complement(9128..10516)
                     /locus_tag="JN749_002891"
                     /pseudo
     CDS             complement(9128..10516)
                     /locus_tag="JN749_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
     gene            complement(10831..11592)
                     /gene="kduD"
                     /locus_tag="JN749_002892"
     CDS             complement(10831..11592)
                     /gene="kduD"
                     /locus_tag="JN749_002892"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HAY7369245.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11622..12458)
                     /gene="kduI"
                     /locus_tag="JN749_002893"
     CDS             complement(11622..12458)
                     /gene="kduI"
                     /locus_tag="JN749_002893"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAY7369246.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(12778..13926)
                     /locus_tag="JN749_002894"
     CDS             complement(12778..13926)
                     /locus_tag="JN749_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HAY7369247.1"
                     /translation="MKDVVVVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            14181..14339
                     /locus_tag="JN749_002895"
     CDS             14181..14339
                     /locus_tag="JN749_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter"
                     /protein_id="HAY7369248.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTY"
BASE COUNT         3803 a         3979 c         3472 g         3431 t
ORIGIN      
        1 gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca
       61 gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat
      121 attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca
      181 gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg
      241 aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg
      301 ttgccgaaat gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg
      361 tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga
      421 gcggaccgtc gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga
      481 atatccgcga agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt
      541 atcaggtgca ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga
      601 cggattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac
      661 gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct
      721 acctgcatct ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca
      781 gtctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg
      841 aactgctggc ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg
      901 caaaacccgc tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc
      961 aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatggc cttgatcctg
     1021 ctcagatggc gctcgcgttt gttcgccgtc aaccgtttgt tgccagcact ctgctgggcg
     1081 caaccacgat ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag
     1141 acgtattagc tgaaattgaa gcggtacatc aggtttatac ttatcctgca ccataaagat
     1201 taacgccggt taaaaccggc gttaaatatt aatgacggcg ctgccagatc cacagcgccg
     1261 ttattgccag cgcaaatagc gcaccgaagc caatgccaat gggcacgacc gggatgccta
     1321 ccattaccgc cagcgagtaa atgcccagca tcaacaacat ggcgctgttt tcgccaaggt
     1381 tttgtactgc aatcgcattc cccgccccga cgcttttttt accccgctcc tgtagcaacg
     1441 cattgagcgg aacgacaaaa aagcccccca gcacgccaat cagcatcaac aaggcatagg
     1501 ctggcagcag ctcgtgttgc agggagaaaa tcagtaccac cacgccaatc aaaatcccgg
     1561 ctggcataca gcgtgacacg gtttccagcg taaccagctt cgctgccgca cctgcgccaa
     1621 ccacgatacc aatcgctacc atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc
     1681 ccagcgccac cggtacccac agcaccaaca ggaaacgcag cgtgacaccc gctccccaga
     1741 ataaactggt gcccaccagc gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg
     1801 cattcaggaa actgcgggtc atgttgatga gattccagga ctgccccgga cgcgccgccg
     1861 ccagtttggg aatgtagata ttggcaacga ccgcaccacc gtaggccagt gcgcatgcgg
     1921 ccagggcgac caggacatgc cagtcagcca gcacaccacc ggctacggaa ccgagtaaaa
     1981 tcgccgctat ggcagaagct tccattaaac cgttagcttt tactaactta ctacccgtgg
     2041 ttaattcgcc gagaataccg tatttcgccg gtgaataggc tgcagcacca acacccacca
     2101 gcgtatagcc gagaaacgga ttgataccaa agcagatact ggctgcgccc agcagcttca
     2161 ggccgttggc aaacatcatc acccggcctt tggcgaagct atccgccacc tgcccgacaa
     2221 acggcgccaa aagaatgtaa gcacctacaa acaccatttg caggatgggc tggctccact
     2281 ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa cagtagcgcg ttatcgccaa
     2341 acgcagagag aaactgcgcc acgataaccg ctttcatccc cttcgaccac aacgaagtgt
     2401 tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt ctacccagct tttcaacgtg
     2461 acaaagtcag gtttgccgct gccaagtaat ggcatctgtt tcagatagcg aatatcgcgc
     2521 ggtacagcaa gctccggcac gccgtgctcg cgggcatact gttgcaactt atcgcgcgtc
     2581 agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt tgctggcatc gctcttaatc
     2641 gcagtggcat ggactttatc tggcgaaaca ccaagtgcca gttgttccac catttccagc
     2701 gacaccattt cgcctgcaat tttggcaaag cgttttgcgc ggccctgaat ctgcacaaag
     2761 ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc agccgcgctc catttcgccg
     2821 cgaacattct cggcggtggg cacttccagt acacctggct tctccacccg cagatagccg
     2881 ttcattatgt tcggcccttt cagttgcagg cgtccgccct cttcgatacc agggaccgac
     2941 aacaggcgtg catccattcc tggtagaata cgccctaccg taccgggttt cgccgccatc
     3001 ggtacgttga tagaaacgac aggcgcgcat tcggtcacac cgtagccttc aaggatgcgc
     3061 aggccaaatt tatcctgcca aagctgtttg gtactttctt gtagtttttc tgcgcctgcc
     3121 accacatagc gtagacgata gaagtcatac gggttggcga aacgcgcgta gtgaccgagg
     3181 aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat acaccaagtc cggcacaatg
     3241 cggtaatgca gcgggcttgg ataaaggaac acttctgcac ctgtaagcag tggcgtaaac
     3301 aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg ccgacataaa gcgatcgttg
     3361 gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg ccagaatgct tttatggcta
     3421 tggacgacgc cttttggatg gccttcagaa ccggaggtaa agaggatcag cgcctcttct
     3481 tccggctgct gttttacctg cgccaggcgc ggcatcagca aatgagcgaa gatccatact
     3541 ttgtcggcag tggtgacatc tgcttttaaa tcttccagat agacccagcg cacctgagta
     3601 agttgctccg gcagatgcca gagtttgcct ttatcgagaa actggcggga agtgaagatg
     3661 gttttgattt cagccgccgt aatagcactg gtcagccctt ttaccccggc agtgtagttc
     3721 atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa tcactgccgc actgatgcct
     3781 gcattgggca gcattaagcc gatgcgttcg ccttcaacac tgtatttttc aaggatgcgt
     3841 ccaacaaaca gcgttttcgt aagcaatttg cgataggagt ctggggtaaa gttgacgtct
     3901 tcgacacatt tcttcccggc tccgaagcgg tacattgcac tcagtaaaga ttcgtacaga
     3961 gtttcacgcg ggcgcaccgc catacgcgct tccatcatta tttgatgcag catttcgcca
     4021 gcgattttgc gacggtcacg ggcacgcggc gcatccggca tcgccacctg cgttggaggc
     4081 aaaatatgca gagtaatttg cgggaacaag cgacgtttaa ccagaccttt caggcggctg
     4141 aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa ccgttgcacc agacttcgcc
     4201 gcgacaaaac ccgcgccatc gtagattttc atcagcgagc ctgtagtggt gatgcgtcct
     4261 tcagggaaaa tcaccactgg tcggccctgt tccaccagac gtaccagatg tttaatagcc
     4321 ataggttgcg tcgggtcgag aggaacaaag tcgataaatg atttcagcca acgcatatac
     4381 cactgttggc ttattgaggt gtaaacggca aacactggac gcacaggtaa aaacagtcca
     4441 agcaaaatgc catcaataaa agagacgtga ttaggcgtaa ttagaacgcg ctcgcccttc
     4501 agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa cacggcacaa atttcgaaaa
     4561 aagctaaaaa gcattccaac tccctttgct ctgattcagt aaaagcgaat ggagggagat
     4621 tacacgagat aaagaacgcg agcgacagta aattaggtgc gaaaaaaaac ctgcgcatcc
     4681 gcgcaggttg gtgcaagaga cagggtacga agagcgtacc gaataatctc accaatcaat
     4741 acctctggga tcaccacttt agcaacctga agccaaacgc caccagcggt caatcgcaac
     4801 agcgtttcgc aaagtgtaac caaaggtttg caatctcttt ttttgtctta cttatttgcg
     4861 tttggctcac attcccacga tgaaaacacg ccaccccttg aaccaacggg cgttttccgt
     4921 aacactgaaa caatgtaagc gtttacccac taaggtattt tcatggcgac cataaaggat
     4981 gtagcccgac tggcaggcgt ttcagtcgcc accgtttccc gcgtcattaa taattcaccc
     5041 aaagccagcg aagcttcccg gctggctgtg catagtgcaa tggagtctct tagctatcac
     5101 ccgaacgcca acgcccgtgc gctggcgcag cagaccactg aaacggtcgg tctgatcgtt
     5161 ggtgatgttt ccgatccgtt tttcggtgca atggtgaaag cggtcgaaca ggtggcttat
     5221 cacaccggta atttcttatt gattggcaac ggttaccaca acgaacaaaa agagcgtcag
     5281 gctattgagc aactgatccg ccatcgctgt gctgcgttgg tcgtccatgc caaaatgatc
     5341 ccggatgccg atttagcctc attaatgaaa caaatgcccg gtatggtgct gatcaaccgt
     5401 atcctgcctg gctttgaaaa ccgttgtatt gctcttgacg atcgttacgg tgcctggctg
     5461 gcaacgcgtc atttaattca gcaaggtcat acccgcattg gttatctgtg ctctaaccac
     5521 tctatttctg acgccgaaga tcgtctgcaa gggtattacg atgcccttgc tgaaagtggt
     5581 attccggcca atgatcggct ggtgacattt ggcgaaccag acgaaaacgg cggcgaacag
     5641 gcaatgaccg agcttttggg acgaggaaga aatttcactg cggtagcctg ttataacgat
     5701 tcaatggcgg cgggcgcgat gggcgttctc aatgataatg gtattgatgt accgggtgag
     5761 atttcgttaa ttggctttga tgatgtgctg gtgtcacgct atgtgcgtcc gcgcctgacc
     5821 accgtgcgtt acccaatcgt aacgatggcg acccaggctg ccgaactggc tttggcactg
     5881 gcggataatc gccctctccc ggaaattact aatgtattta gtccgacgct ggtacgtcgt
     5941 cattcagtgt caactccgtc gctggaggca agtcatcatg caaccagcga ctaaccgcag
     6001 ttaaagcaat tccagcgcca gtaattcttc gatggtctgg cgacggcgaa tcaaccgcgc
     6061 ctgaccatta tcaaacagaa cttctggtaa cagcggacgg ctattgtagt tggatgacat
     6121 tgatgcgcca tatgcccctg tatcatgcag taccagataa tcacctgcct tcacttccgg
     6181 caaggcgcgg gtttcaacat ttcccccttc ctgctgggta aagacatcgc ccgattcaca
     6241 taacggcccg gcgacgacgg tttccaccgt tggcgcgtgt tccagagaac gaccatcagc
     6301 tgccagggca ctgatatggt ggtaactacc gtacattgcc gggcgcatca gatcgttgaa
     6361 cccggcatca accagcacaa agtggcggct acccatttgt ttgacgctcc gcacctgagt
     6421 aattaatacg ccagactgcg ctaccaggaa gcgacccggt tcaatttcca gtttcacagg
     6481 gtggcccaaa tggcgagcga tttgctcacg cgcggcattc cacagaccat aataatgttc
     6541 ggtatcaacc gcctcttcac cctgttgata aggaatagaa agcccaccgc ccgcagaaat
     6601 agcctgtaaa tcctgaccga attcgatgac ctgacgcacc atagcaccac acacctgctc
     6661 cagatgggca taatcaacgc cagaaccaat gtgcatgtga atgccgacta gctgcagatg
     6721 atgacgttgt atcacgtcca gtgcggcggg cagatcggtg taccagatac cgtgcttgct
     6781 gttttcgcca ccggtattgg ttttttggct atgtccgtga ccaaaccccg gattaacgcg
     6841 cagccatacc cgatgccctg gcgaaacctg gcccagttgg tcgagcatat caacagaacc
     6901 cgcattcacc ggaatttgca attcactgac gcgttcaagc gtcgcctgat cgataacatc
     6961 tgccgtaaaa acaatatcat cggggtgcgt ttgcggattg taacccgccg ccaacgcacg
     7021 ctctatttcg cctaacgaga cggaatccac tttcacgccc tgctcacgca ttaagcgcaa
     7081 aatatgaata ttggaacagg ctttctgtgc aaagcgcacc acttcaaact gtttcagcgc
     7141 tgctatctgc cgacgaataa tttgcgcatc gtagacccac accgggcagc caaattcagc
     7201 gggcaaacgc agcagatttt cggcggtgag atcggtatcg gtgctgaaca gtgaatgtgg
     7261 cataacaaac tccagatgag tgctttttta tgattacgcc acatcataaa aagaataaaa
     7321 aatatcgatt tatgtcgaat ctatgcaaaa atgatatgga ttaccggatt gcgagagagc
     7381 gctaatggcc gccgttaact tacgtcatat tgaaattttt catgcggtaa tgaccgccgg
     7441 aagcctgact gaggcagcac acctgctaca cacctcacag ccaaccgtca gccgcgaact
     7501 ggcgcgcttt gagaaggtga tcgggctgaa attgtttgag cgcgtacgtg gacgattaca
     7561 tcctaccgtg caaggactgc gtctgtttga agaagtgcaa cgatcctggt acggactgga
     7621 tcgcattgtc agcgccgcag aaagtctgcg cgagtttcgc cagggagaac tgtctattgc
     7681 ctgcctgccg gtcttttcgc aatctttttt accgcagctc ctgcaaccct ttctggcacg
     7741 ttatcccgat gtcagcttaa atatcgtgcc ccaggaatca ccgctacttg aagagtggct
     7801 ctcggcccag cgtcatgatt taggactcac tgagacactc catacgcctg cgggaacaga
     7861 acgtaccgaa ttactctctt tagatgaagt gtgtgtgtta cctccgggtc atccgctggc
     7921 ggtaaaaaag gtattaacgc cggatgattt tcagggtgag aattacatca gcctttcccg
     7981 tactgacagc tatcgccagt tgctggatca gctatttact gaacatcagg ttaaacgacg
     8041 catgatcgta gaaacccaca gcgccgcgtc agtctgcgca atggtacggg cgggggtagg
     8101 tgtttcggtg gttaacccgc tcaccgcact agattatgcg gcaagcggtt tagtggtgcg
     8161 gcggttcagt attgcggttc cgttcaccgt cagcctgatc cgccccctgc accgcccgtc
     8221 atcagcgctg gttcaggcgt ttagtgggca tttacaagcg gggttaccga aactggtcac
     8281 ttctcttgac gctattttgt cgtcagctac gacagcataa aagcgacagc atcctcggca
     8341 tggatcgacg cggtatcaaa cacaggcaaa acactgcgct cttctggcac cagtaaacca
     8401 atttctgtgc agccaaaaat gacgccctgt gcgccctgtt ctgcaaggcg agcaatcact
     8461 tgcgcataat aagcgcgtga cgcttcggta aatttcccca gacacagttc ttcaaaaata
     8521 atctgattaa ttttcgcccg ttcatccgct tcaggaataa gacaattgat ggaaaattgt
     8581 tccgtcagcc gcccgcgata aaaatcctgt tccatggtgt aacgcgtacc cagcagcgcc
     8641 acacgagtca ttccggcccc ggtaattgca cgtccggtgg catccgcaat gtgtaaaaaa
     8701 ggcagagtgc aacgtgactc aatggcatcc gccactttat gcatcgtatt ggtacataac
     8761 acaatacctt ctgcgcccgc ccgctgtaag ccaagcgccg cctcagccag aatgtccccg
     8821 gttttatccc attccccgcg acgctggcac tcttctattt catgaaaatc gacgctatgt
     8881 agcagcattt gcgcagagtg aagcccacca agccgctgtt taatgccttc atttatcaga
     8941 cgatagtaag gaatggtgga ttcccagctc attcctccca gcaaaccaat tgttttcatt
     9001 cctctctcct gatgtgtgtt accgcaacaa ggctgaataa ggaacgagac aaacgcctca
     9061 acggccaggt gcccaatctc tattaacgaa aaaagggccg gatgtacagc acatccggcc
     9121 cgtgaaatca gacgccgata tttctcaact tctcgcctgc catcagtttg cgttcgatat
     9181 gttccagcgt gacatttttg gtttccggaa tgagccagaa agtaatgccc acaaacgcaa
     9241 tgttcaacgc agtgtagagc cagaacgtac cggcagcgcc aatgctatca agcagtgtca
     9301 ggaaggccgc gccgataatc atattcgaca cccagtttgt cgtcgtcgaa caggtaatac
     9361 cgaaatcgcg gcatttcagc ggctgaattt cagagcacag gatccacacc actggcgcgg
     9421 cgctcatcgc ataaccggca atacacatca tcgtcatgcc aacagagagc caggacaagc
     9481 cactggaagc cgtaccgtta tcaaactgca tcaggcaata gcccagcacc agagtgccta
     9541 acgccatcac gctgaaacca attttcagag ccggtttacg acctgcttta tctaccgtaa
     9601 agaccgcaat gaaggtcgcg aacataaagg tcagtcccac gaccagagtc gcaatcatct
     9661 gttgttctgt ggtcgtaaag cccgccattt tgaagatacg cggcgcgtag tacatgatga
     9721 tgttcatacc ggtaaactgc tgcatcgcct gcaacaacat accgagaaac acagcacgac
     9781 ggacgttacg gttgatctta aacagtgccc aaccgccctg ttttaacttc aggctttcac
     9841 gaatttcgtt gagttcttct cgcgcttttt ccgacgtatc gcgcagcata cgcaacactt
     9901 cttccgcctc aatatgacgc cccttttccg ccagccagcg cgggctattt ggcaggaaga
     9961 ccaccagaat aatcagcaga actgctggta aagcaagaac cccaacattg cgcgccagtt
    10021 accgctataa ctgaacgctg tatcggataa aaacgccagc acgatgccga gtgtgaccat
    10081 caactggtac atactgatca tcttaccgcg aacgtacttg ccatttcaga aagatacaga
    10141 ggagcggtgt aagacgcgat cccgacagca atgcccagca ccacacgagc ggcgattaac
    10201 atctctacgc tggtcgcaaa agcggaccct atagaaccga gtacaaacag gatggcccca
    10261 ggcggaacga cagccaacca ttaaacagcg caccaattgc tgcgccgagc atcatgctac
    10321 taaccaccca ttcctgcaaa cgactggtca gcacaaagtg atcggtaatg aacggcaacg
    10381 ctccggcgat tacgccgata tcaagaccaa ataacaatcc tgcgaccgca gcagctaccg
    10441 aaacaaacat attcatacgc cgcgtatcac gcaaagaacg tggcgttaaa gcagattccg
    10501 tattgatagt aaccattttt tcctgccagc agagagtaag acattgtgaa aaaatacgtg
    10561 aacaattcac acaggtgtca ggtcggaaac agcataaata tggattaaat tgctgcgaca
    10621 tgccgttatg tgatggatat tccaatttta aaattaagtt gaattattga gattattatt
    10681 aaccacctaa ttttaaagta gataaaattc ataaagttca ttaattgata attgatatgg
    10741 attatttcat aaccatgata tggattatga tgatctagag atataaaaaa ccctgccatg
    10801 cggcagggtc ataaaagtaa gaggaatgaa ttaacgcgcc agccaaccgc catccacagc
    10861 aatggtataa ccatttacat aatctgaagc gctggaggca agaaacacta ccggccccat
    10921 caggtcactc ggcagtcccc aacggccagc tggaatgcga tcgagaattt ccgcgctacg
    10981 ttgttcatct gcccgcagtt gttgggtatt gttggtcgcc atgtaacccg gagctatcgc
    11041 attaacatta atgttgtgtt tagcccattc gtttgccatc aatcgcgtca cacccatcac
    11101 tccgcttttt gatgcggtat aagaaggcac acggatcccg ccctggaagg agagcattga
    11161 cgcgatattg ataatcttgc cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg
    11221 agacatgaag aatacgctct tgatattcag gttcatgaca tcgtcccagt ctttttcgct
    11281 gaactcgaga gcgtcttcgc ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg
    11341 accaaattca gctaccgcgc gatccagcag tccaggaatg ccatcaatct ttcgcagatc
    11401 ggcggtcagg cttaaaaaac gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg
    11461 ttcaacgatg ttaatgccaa caatgtcaca gcccgcttgc gccagcccca acgccatccc
    11521 ctggcccagc ccagtatcac aaccagtgac gaccgcaact ttaccttgga gagaaaatgc
    11581 acttaaaatc ataacaacac cttattcgtt tatgcccaca actagcgcaa atctttaacg
    11641 gccacatggt ccatatcatt aaagacctgg ttttcaccga ccatgcccca gataaaggta
    11701 taagctttgg ttccgacgcc ggaatggatc gaccagctcg gggagatcac cgcctgctcg
    11761 ttatgcatca caatgtgacg cgtttcttgc ggctgcccca tcatgtggaa aacgcaggcg
    11821 tcatcatcca tattgaaata gaaataaact tccatccggc gctcgtgggt gtgacacggc
    11881 atggtgttcc acaagttacc tggagccagc tccgtcagcc ccatactcaa ttgacaggtt
    11941 tccagtacat ccgggacaaa atatttgtta atcgtgcgac ggttactggt gaggttatcg
    12001 cctaacgtga ctggagatac ttcgtccggt gtgacttttt tggtgggata cgtcgtatgc
    12061 gcgggtgcgc aattgtaata aaacttcgcc ggagtggcgg tatcaatact ggcaaagaca
    12121 acttcttttg cacctttacc aacatacagg gcgtcgcggt gaccgatttc atagcattga
    12181 ccatcgacag taatcgtacc ggcaccgcca atattgataa cacctaactc gcgacgttca
    12241 aggaaatagc ttacgccgag ttgtttacca acttccccgc caacggaaac cgttttagtt
    12301 atcggcataa tgccgccaac aataattcgg tcaatgtggc tgtaaaccat ggtgtactca
    12361 tcggcgacaa ataccttttc aaccaaaaat tcattacgca gcccttgggt atccagcgtt
    12421 tttgcgtgcg caccgtggat gctctgtctt acgtccacat caacctccga taatcagtga
    12481 agcgtgaaaa tagaaaacga tagtgaaaca ctgtttcgtt ttctctataa agggatgata
    12541 tgcccatggg ctagggtttt caattaaaaa taaaacaatg ttttattttt aatttaagga
    12601 ttaaaaaaag tgcagtgtcg atcacgaata tgtcatgaga atggaaaaat gagggagccc
    12661 agaaaatgta tgtcgacttg ccagccatca ttattcaaca gattaatgac ggcagatatt
    12721 tatcggtaag gaggaaatgc agagctactc gtcacgttca atggtcaatg ccacgcccta
    12781 gcccccgccg atacaaagcg ttgccagccc tttgcgggca ttacgtttca ccatttcatg
    12841 aaccagagaa accaggattc ggcaaccgga agcgcctatc gggtgaccga gtgcgatcgc
    12901 gccaccattg acattgaccc gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc
    12961 ctgtgcagca aacgcttcat tagcctcgat aagatcgaca tcagccaact gccagcctac
    13021 acgctccagg caacggcggg tcgcatacac cggcgcaatt cccatcaatg ccggatctac
    13081 accaacgctg gcaaatgcgc ggatgcgggc cagcacgggt aaattcaacg ctcgtgcttt
    13141 ggcttcgctc atcatcatta ccgcagctgc gccatcgttt atggatgatg cattccccgc
    13201 ggtcacagaa ccgagactat caaatgaagg atttaaacgg gctaaacctt ctgcactggc
    13261 gtcagtgcgt ggctgttcat cggtatcaac aaccaacgtc tgaccgttac tttggatcat
    13321 taccgggacg atctcatctt taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg
    13381 cgagctaagt gcgtaagcat cctgcaactg acggctgatg ccatattcgc gagccagatt
    13441 ttcggcggtg acaccaatat gataatcatt gaaggcatcc cacaacccat catgcaccag
    13501 actgtcaacc aactggctat tgccaagctg tgcaccggtg cggctatcag tcagaacatg
    13561 tggtgcgcgg ctcatgtttt cctggccacc ggcgatgaca atatcagcct cgccacactg
    13621 tatcgcctga gtagccagat gcagtgcttt aagaccggaa ccgcaaacgt cattaatcgt
    13681 gattgcagaa acgctgttag gaagaccacc tttaatagcc gattgccttg ccggattctg
    13741 ccctgcccct gcagtcaaca cctgaccaag aattacttca tccaccgcat atgcaggaac
    13801 gccggtacgt tctattaacg ctttcacgac cagactacca agttccacgg cggaatgacc
    13861 cgctaacgca ccacgaaagc agccgatagg tgtccgtaac gccccgacaa ccacaacgtc
    13921 tttcatcaca acctcgcacc aattaacagt gcaatagtaa atgattgtta attacagtta
    13981 tcttaattgt ttaaaaaaag tgatttttat cacaaaggaa atatgcctga gcagtagtca
    14041 gagtcataac tggcacgtaa ggtttgcaac cactaaccca ccaatagatg ggtagatagg
    14101 gcgttaatct cccatactta acttggttta tggtaaattg ccctccattt tgtttaattt
    14161 gtagatgata cgttcagata atgtctaata tttggtctaa agaagaaact ctgtggagtt
    14221 tcgcgctcta cggcacagcc gttggtgcag gcacgctatt ccttcctatt cagttaggtt
    14281 cggcaggggc tgtggtcctg tttattactg ctctggtcgc ctggccttta acatattagc
    14341 cacataaagc cttatgccag ttcatcctct catcaaaaac atcagcaggt gaagggataa
    14401 cgggcgcggt aacacactac tatggcaaga agattggtaa tctgattacc acgctgtact
    14461 tcatcgcctt ttttgtcgtc gtgttgatat atgcagtggc aattaccaac tcacttacgg
    14521 aacagctggc aaagcatatg gttattgatc ttcgcatccg tatgttggtg agtctgggtg
    14581 ttgtattaat tctgaatctc atttttctga tgggacgtca tgccactatt cgggtaatgg
    14641 gatttttggt attcccattg attgcctatt tcttatttct ttcca
//
LOCUS       DADLTF010000104        14252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.104, whole genome shotgun sequence.
ACCESSION   DADLTF010000104 DADLTF010000000
VERSION     DADLTF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.104"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..106)
                     /locus_tag="JN749_002896"
     CDS             complement(<1..106)
                     /locus_tag="JN749_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HAY7369249.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="JN749_002897"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="JN749_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HAY7369250.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="JN749_002898"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="JN749_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HAY7369251.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="JN749_002899"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="JN749_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HAY7369252.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="JN749_002900"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="JN749_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HAY7369253.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSVLQNNSTDF"
     gene            2868..3643
                     /locus_tag="JN749_002901"
                     /pseudo
     CDS             2868..3643
                     /locus_tag="JN749_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="JN749_002902"
     CDS             complement(3707..4198)
                     /locus_tag="JN749_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HAY7369254.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /locus_tag="JN749_002903"
     CDS             complement(4300..4854)
                     /locus_tag="JN749_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7369255.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /locus_tag="JN749_002904"
     CDS             complement(4878..5615)
                     /locus_tag="JN749_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HAY7369256.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="JN749_002905"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="JN749_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6842..6929
                     /locus_tag="JN749_002906"
     tRNA            6842..6929
                     /locus_tag="JN749_002906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6876..6878,aa:Ser,seq:gga)
     gene            complement(7079..7360)
                     /locus_tag="JN749_002907"
                     /pseudo
     CDS             complement(7079..7360)
                     /locus_tag="JN749_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7642..8646)
                     /locus_tag="JN749_002908"
     CDS             complement(7642..8646)
                     /locus_tag="JN749_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HAY7369257.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8718..9278)
                     /locus_tag="JN749_002909"
                     /pseudo
     CDS             complement(8718..9278)
                     /locus_tag="JN749_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9363..9560)
                     /locus_tag="JN749_002910"
     CDS             complement(9363..9560)
                     /locus_tag="JN749_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HAY7369258.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9572..10063)
                     /locus_tag="JN749_002911"
     CDS             complement(9572..10063)
                     /locus_tag="JN749_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369259.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(10060..10434)
                     /locus_tag="JN749_002912"
     CDS             complement(10060..10434)
                     /locus_tag="JN749_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAY7369260.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10481..10855)
                     /locus_tag="JN749_002913"
     CDS             complement(10481..10855)
                     /locus_tag="JN749_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HAY7369261.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(11018..11239)
                     /locus_tag="JN749_002914"
     CDS             complement(11018..11239)
                     /locus_tag="JN749_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HAY7369262.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(11302..11778)
                     /locus_tag="JN749_002915"
     CDS             complement(11302..11778)
                     /locus_tag="JN749_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HAY7369263.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11794..12267)
                     /locus_tag="JN749_002916"
     CDS             complement(11794..12267)
                     /locus_tag="JN749_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HAY7369264.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12609..13430)
                     /locus_tag="JN749_002917"
     CDS             complement(12609..13430)
                     /locus_tag="JN749_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HAY7369265.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13530..13703)
                     /locus_tag="JN749_002918"
     CDS             complement(13530..13703)
                     /locus_tag="JN749_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HAY7369266.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAEI
                     DAGRFASDGLLRQ"
     gene            complement(13823..>14252)
                     /locus_tag="JN749_002919"
     CDS             complement(13823..>14252)
                     /locus_tag="JN749_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369267.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3641 a         3494 c         3408 g         3709 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtctttcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc ggttttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattattgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gcaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa acccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcatcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaagtttgaa taaacgcgcg attctttcaa aaagtttgtt
     6781 gacctcaggt catgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt
     6841 tggtgaggtg tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat
     6901 cgggggttcg aatccccccc tcaccgccag attataagtg catgttattg tttaacatgc
     6961 gcttttttat ttttccttcc agacagtatg ccatcgggta tgccatctga aaccggttta
     7021 tatgccccgc gatcatatct tatcggacaa tttccgatct ttgagcatac tggcaaagct
     7081 atactgccag catatcagga tagtgttttg cgataatatc attcagtctg tcaaccagtt
     7141 ctggttttct gaacgtgata tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
     7201 catcttcata gctctcttta ccctgaacag actgaatgtg atcatccaga cgaagggtga
     7261 tgtttccccg gttgtcgggg attggtttac cgctgaacag gtgcaacatt ctttccagat
     7321 cggcgagtcg gtcagcctgt tgtccggtga taagatgaaa tctatggtca cccccgtttt
     7381 tgcaacactg atttttgatt aatggttggc ttgcttaaat ctatccggcg tctctgtggg
     7441 caaggatgcc cgcgccccga tgagttccgc acctgataat cctaaaaatc ccctcggctt
     7501 cagtgagcca tttcatctgt taggttttta atcaggtcga tttgccgttc atgtcatcaa
     7561 taatcagtct tcgcaaaacc agaaaggtaa cttgtatctt attgtttaaa ataaactaat
     7621 tgagttatat cagcatctat atcaggcctg atattctttt ttctgagtag tgattgccca
     7681 ggcaattcga gccagtttat ttgccagagc gcaggtaacc acattcgaat gcttcttgtt
     7741 gagttgctcc cgaacccact cagcaagttt tccatgcttg cgttcaagtc gcattatgaa
     7801 tgagcgggca cactgaacca gtaaccggcg taaattttta tcacctcgct tactgatacc
     7861 caacagagtc gattttcctc cagtgctata ctgccgggga acaagtcctg tggaagctgc
     7921 aaaatccctg ctgcatgaga actgtttccc gtcaccaagt tgcgacgata gtaagctggc
     7981 agtaatcgga ccaacaccag gaattgtcat aatgcgctga gcggtatcat cagcgttgat
     8041 tgactgactt aattctgatt ccagctctgc tatttgctca acaaggtaaa agtagtgggt
     8101 atgcaacctc accaaaaggc ggctcagata ttcggggatt tcgtgttcag ccaacacgag
     8161 agacaaccgt tttattacag catcacctat cggcaggctg attccaaatt ccagcaagaa
     8221 accgtggatc tggttggttg ttttcacctt atctctgatg agcgattctc tgacccggtg
     8281 taacgccctc atggcctgct gggtctcagt tcttggctgg acaaaacgca ttgatggtcg
     8341 tgaagccgct tcgcatatgg cttctgcatc aacaaaatca ttcttattac ttttgacaaa
     8401 cgggcgaacg aactgtggag aaatcagctt agtttcatgc ccaaaatcag agatacagcg
     8461 agccataaag tgtgcacccg cacaggcttc cattacaaca actgagggcg gacatgtcgc
     8521 cagaaactgc atcagtttag tacgggtaaa ttttttacgc agaagagcct taccagactt
     8581 gtcttgacaa tgaatgtgga aggagtgctt tccgagatcg ataccaataa gtgtgatgtt
     8641 ctgcatgatg gtctctccgt taaagggatt taccttaaca gcgtactgct gttaaggtgg
     8701 gggtgaccat atcattatcc ccaccggtcc catctcacca gattgttgac gataatttta
     8761 ctgccaaatt tacaggggca attggttttg taattccagt tcagccctct gaagacgttg
     8821 atcacccctc gctcaaacac ctcatcctta ttctggtgca gttgttcaaa tgtgctcaga
     8881 atgttcgctt cgctgatttc cggaaaatta tcgtactcca gtgaccggta ccatgtatca
     8941 cgggcctgcg tatccattaa tgacagcatc ccggaacgtt gcatcaggtc acgccagatt
     9001 tcgcgatcaa gattccgggt aatggctttc attgctgttt cgggtttctc catcagccag
     9061 cagccgcagc gaaaatcctg tttcatggcc cagtccgggg cagttttacc tccgatgctt
     9121 tccgtcagca ccgagatttc agccagttgt cggataagtg tgtcgatttg caacagtgta
     9181 ctctttcgta cagccagaat gtggcgaatg ctggttgagc agataacgtc cgtatgcccg
     9241 ttgaccactt ccggacggga gattgtgata tctgacataa tcgatattcc tttttgcaga
     9301 taaatgaaaa cgccagccct gtgcaggctg gcgtcagagg aatgaagtct ggtgagtgag
     9361 cttcaccggt tctggtgcag aagctgcaga tgacgcaggg tacggacatc ctcccgggcc
     9421 tgttcgatac agggcaacag tgctgctgaa tccgttttat cctcatcgtt gtcaaagata
     9481 attcccgatc cgcagtcgat attgtcctgc agccacgcaa tcagaatatc cagcgctgtt
     9541 tccgtggtta atgatttcat gttgttcctg gttaccagtc gaaagtgggt aaacccggca
     9601 ggcattcagc gctggcatcc agatgaatga gactgacgcc ataacgccgg atgagtgtga
     9661 cgaccagacg acggaacgtt tttgacagcc ccaggcgttt aagccgcaga accgggtatg
     9721 accacgcatc ggtacgtaac agatatccgc tacctgtaaa atgaatccat tctgattcac
     9781 cgaagtcact ggtctggtgt gacagcgagt acaaccaggc gttgtccgtt tccgtgatat
     9841 gtgcggtact gcagcgtatg ccggtaaggg tagcaaatgg tggcgtgcat tctgctgagg
     9901 tctggtcaga ttcatcaaca acacgaaggg agtagccgtt atcgcagacc atgttaatca
     9961 gttcagcgag ctcaatacca tcgacgtcaa cgacaatgcg ccctatgttc agtgcctgta
    10021 cgttaacgct gtcggcttcc agtgtcaggg ccagtttcat catttcgcct ccggatactt
    10081 acccagggta atgttattta ccgttctgta attgtcgcgg gtcatcaggc cggtcgcccg
    10141 gcgagcccgg aggatatcaa tactgtttat taactgagag cggggacagg tgctgaatcc
    10201 cggctggtcg gtacgcacca gcgcgtattt ttccacgaga aaattaactg catcacacag
    10261 tgaaatgcct gcctcaatat gctgctcaat cacacgttca tcgacaaacg gtgtgtcatt
    10321 cagtgtgagg ccataatgct ggtccagcag tcggatgagc agtgtctgcc agatggtgac
    10381 gggagacggg cagcgagata cctcccgtac atgtgtgtcg gataatgttt tcatcttaag
    10441 ttctctgtaa ctggtagtgc taaaggtgag agcttcgggg ttatgaggtg gttgcgggtg
    10501 ctggtgtcgg ataaacagcc agataaacgt aaccacagga gccgagggtg tcggcttcgc
    10561 aggtcagccc ttttgcatac agcgtgacgg tatgctgata tcggggattc agttcaccgc
    10621 tggtgagcat gagttccagt tgtttcatca gcagcggaaa ggcctggtcc agatggtatg
    10681 catccgcatt gctgaaccgg cctctgatac cggcgcggtc tgcaaggtaa tacaggcggt
    10741 taccctcttg caccagacga gcgccaaaac agggcctcac tgtgcaggga agcccccacc
    10801 aggggcggtc gtgattgtca tcgggatgag ttatcccgga gagtttgtct gacacgataa
    10861 aatcctctct gaaaagtggt taagaatggt ttaacgatga caggtcagcc ggcgcgctga
    10921 ccggtttcgg tgaagatgta tgcattgatg agaatggcct catccacagc ggcgtctgag
    10981 gtcatctggt cgtactcgtt tcccgggtga cggaacatca gttcagggtg acactcatca
    11041 gccggacata cgggccaaaa ctgtccttac ggcgttctgc aaacacggca agaacgccgg
    11101 gaatatcctg cacttcacga ccagtatacg cttcagcact gccgtgccag cgatacttac
    11161 cggtacagaa cggaaaaaga cgggatgcag gatgctgttg gtgaatacgc atggcttcac
    11221 cacgggtgat gattctcatg atgtgatacc tctgaagaca gaagataaaa gtgaaaacag
    11281 gtgtgatgtg gttgtgacgg gttaaagcag accatgttcg gcaaaggaga aaacctggtt
    11341 gccaccgact atcagatggt ccggcacccg gatatccacc aggcccagtg cctgtaccag
    11401 acgttcggtg ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg
    11461 cgccagcacc acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg
    11521 aacttccgta cggttgatgg tgccggtgaa gagggtttca ccggcaatca gctgattctg
    11581 gttgttcaga tacagcaccc ggaactcttc ccgctccagt cccgccatgt tcagaatcag
    11641 ccattcccgt gccgcacggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc
    11701 cagggtttgc agggcccgca gaatgagact gcgctcgccg ggcgtcatgt ttccgggcag
    11761 aaaggaaagc tgctgcattg tccctgcctc cattcagtcg atgatgcgca taatggcgct
    11821 gcattccgga tgctgcaggg cgtaatcccg cagccggtaa taatggaccg tcatggcata
    11881 acactccgta cgacaggcat gatgactgta cgtcatcaga caggcagcaa tgccggcggc
    11941 ttccgggctc atttcagcgc ggttaccgtt catggcattg aacagtaccc atgtttcgtc
    12001 atcatcgtca tccggttcgg gtgccataaa tgccccgccg ttgttcaggg tgtacagatt
    12061 ccagatacca ccgcagtagt cttcacacag acggtccatc cagccgaaga cacggggttc
    12121 cagggtgacc cactgtggaa tgaggccaaa atgctgcggc cagaagctga tgcgctgttc
    12181 atcagggacg ggagtggcaa ccagttgttg caggccagtc tccggtgcac aggccgaagc
    12241 ggtggtgttc tgagacaatg ttttcatgta tgaaatcctt gtaatgtgaa taaatgagag
    12301 ggagtaaagg tcgggagctg gcaacatcac gttcatcact gaccgtgatg acccggtaat
    12361 atcagggatg aatgatattc ggctgaaatt ccggacagcc gtacgcgtaa ccgttccggt
    12421 cacagacgga tggccagatg ccattgcctg tatactcagt ctgcacagac tgatttctcc
    12481 gggacggtaa agcagacggg tacaccaggc gcacaggtca tcatcagaaa tggaacgaca
    12541 ccccggcagc gcctccgggg cgtcgggtga ttctggtgcc tgaatcacgc cttcagggac
    12601 atgctgcctc agcgcaggct ctccagcagc gtttctgcca ttacccacaa tgcgcggttg
    12661 agcttaatat cggtgtcgat gttgtgaatg gcacgggtgt ggatacgttt tccttttgca
    12721 ctgcggccgg aaatcccgcc tttcagcata ttctcctgga tggtctgata tgcgctccac
    12781 aggtctttac cgtaatcctc cctgcgtcgt ggtgtcagaa tgtcagcggt ggtgactggc
    12841 tgatgttcgt caccataacg gtaagtcagt gccgcctgtg ccagcgcctg gcgtgccggt
    12901 ggcggcagga ccagcgactg catggcatca cgcttctcct ctatccggtc aaacacgccc
    12961 accacctcgt aagccccttc aataactttc tccaccacat ttccccggtg tggaacacgc
    13021 aattctccca gagactgacc gcagaccagt ccattggtac aaatggcacg aaaatatccg
    13081 ggtaacatct ggtagctgga tgaaccgtca tgagagttga gcagaataat ttcagggaca
    13141 tgctgaccgg ttatctgtcc ggcccgccgc agacgcagca tgtgttttgt gtattcccgg
    13201 cgacccgggt cacgcacacg tgtctggcag gcgaagaatg gctgaaagcc ttcccgctgc
    13261 aggctttcca gtacggtgat ggtgggagtg tacgcatagc gtttactgcg ggaagtatgc
    13321 cggtcttcac cgaaaatact gggaacatag tgcatcagtt cttcgtgtgt cagcggacgg
    13381 tcacggcgta tctggtttgc agcataacca aaacgactgg ctagtcgcat aatttgctcc
    13441 ttatcggtgg ttaagattta ctggtgtaat aaatgaaaaa gccacgtctc ccggagaaga
    13501 cgcggcctga tggacgaaat taatgaggtt tattgtctga gaagaccgtc actggcgaac
    13561 cttccggcgt caatctctgc gttgcggata acccgacgga aatgagttcc ctggtcatcg
    13621 tcgataaaca cattgcggta agctgttgtg atgacaacag cctgctcgcg tgagaaaggg
    13681 acttcgggca ataaacgtga catacaacca gacctgtatt tggttccctg aaaatataag
    13741 tccctgtcat catggcagag ccgtatttac ttttgatatc aataaattaa taacgcgaag
    13801 atgaaccgca taacttacgc ctttaatttg ttttgtttac gtagtccctg accaggtacg
    13861 cgaattcgtg aacgccagtt tcaccaaacc aaatcaggta accagggtcg atgttgtaat
    13921 agtaaccaac gactttatgc gtttttgggt tatagagtgt tacgatatag tcccagccgc
    13981 aggcgtgggc tttacaaccg ccaccttcta cccaggtcgt tccgttaatg tttacaggat
    14041 acgcaccacc atcaggacca tccatatagc cgtcttcccc aaacattccg tttaacccaa
    14101 cagggtttaa tgctttgtcg atagcagctc tgtaggtagc gtcaccttcg tataactcgt
    14161 acgttgttgg acatgcacca ggctggtcag cccgggcttt tgagtcgcat ttaagtgtct
    14221 gatttacagt ggcggcgttt gtgtcaggac tt
//
LOCUS       DADLTF010000105        14000 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.105, whole genome shotgun sequence.
ACCESSION   DADLTF010000105 DADLTF010000000
VERSION     DADLTF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.105"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..170
                     /locus_tag="JN749_002920"
     CDS             <1..170
                     /locus_tag="JN749_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7369268.1"
                     /translation="RINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPK
                     RYRKKLTEFNQ"
     gene            275..1627
                     /locus_tag="JN749_002921"
     CDS             275..1627
                     /locus_tag="JN749_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAY7369269.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1640..2398
                     /gene="chbG"
                     /locus_tag="JN749_002922"
     CDS             1640..2398
                     /gene="chbG"
                     /locus_tag="JN749_002922"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HAY7369270.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JN749_002923"
     CDS             complement(2445..4706)
                     /gene="katE"
                     /locus_tag="JN749_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HAY7369271.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIREHVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4889..5152
                     /gene="cedA"
                     /locus_tag="JN749_002924"
     CDS             4889..5152
                     /gene="cedA"
                     /locus_tag="JN749_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HAY7369272.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5429..6245
                     /locus_tag="JN749_002925"
                     /pseudo
     CDS             5429..6245
                     /locus_tag="JN749_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6249..7639)
                     /locus_tag="JN749_002926"
                     /pseudo
     CDS             complement(6249..7639)
                     /locus_tag="JN749_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
     gene            complement(7772..8362)
                     /locus_tag="JN749_002927"
     CDS             complement(7772..8362)
                     /locus_tag="JN749_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7369273.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="JN749_002928"
                     /pseudo
     CDS             complement(8525..9192)
                     /gene="hxpB"
                     /locus_tag="JN749_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            9339..9875
                     /locus_tag="JN749_002929"
     CDS             9339..9875
                     /locus_tag="JN749_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369274.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(9916..10776)
                     /locus_tag="JN749_002930"
     CDS             complement(9916..10776)
                     /locus_tag="JN749_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAY7369275.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPSFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="JN749_002931"
     CDS             complement(10882..11172)
                     /gene="ghoS"
                     /locus_tag="JN749_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HAY7369276.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="JN749_002932"
     CDS             complement(11273..12202)
                     /gene="pfkB"
                     /locus_tag="JN749_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HAY7369277.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            12488..13246
                     /locus_tag="JN749_002933"
     CDS             12488..13246
                     /locus_tag="JN749_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HAY7369278.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(13299..13406)
                     /locus_tag="JN749_002934"
     CDS             complement(13299..13406)
                     /locus_tag="JN749_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369279.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(13578..>14000)
                     /locus_tag="JN749_002935"
     CDS             complement(13578..>14000)
                     /locus_tag="JN749_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369280.1"
                     /translation="HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLL
                     KGATAQGPPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSA
                     VHIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
BASE COUNT         3612 a         3217 c         3456 g         3715 t
ORIGIN      
        1 tccgtattaa ttttgccaaa aaacaactgg aaatgaccaa ctattccgtg acggatattg
       61 cgtttgaggc cggttatagt agcccgagtt tgtttattaa aacgtttaag aaattaacgt
      121 cctttacgcc taagagatat cgtaagaaat tgactgaatt taatcagtaa gtcgttatac
      181 ctgacaattc acatatctgt cctgttgctg gattatttat gtccgggggg gcagatatgc
      241 cagatatcag tattctgtac tgaagggaga aattatgagc cagaaattaa aagtcgtcac
      301 tattggtggc gggagcagct ataccccgga gttactggaa ggatttatta agcgttatca
      361 cgaattgccg gtcagcgaat tatggctggt ggatgtcgag ggtggcaaag ctaaactgga
      421 tattattttc gatctctgcc aacggatgat tgataacgct ggcgtcccga tgaagcttta
      481 taaaacgctg gattgccgcg aagcattgaa agatgctgat ttcgttacta cccaactgcg
      541 cgttggccaa ttaccggcgc gtgaactgga tgaacgtatt ccattaagtc atggttatct
      601 tggtcaggaa accaacggcg cgggcggttt gtttaaaggt ctgcgtacca ttccggtgat
      661 ttttgacatc gtaaaagatg tcgaagaact ttgtccgaat gcatgggtga ttaacttcac
      721 taacccggcg ggaatggtta ctgaagccgt ttatcgtcat accggattta aacgctttat
      781 cggcgtgtgt aatattccga tcggcatgaa gatgtttatt cacgatgttc tgatgctgaa
      841 agacagcgat gatttatcta tcgatttgtt tggcctcaac catatggtgt tcattaagaa
      901 tgtgctggta aatggcaagt cgcgctttgc cgaattgctt gatggtgtgg catccgggca
      961 gttaaaagca tctggcgtta aaaatatttt cgatctgcca tttagcgaag gcttaattcg
     1021 ttctctgaat ctgttgccgt gttcttattt gctttattac ttcaagcaaa aagagatgct
     1081 ggctattgaa atgggcgaat actacaaagg cggcgcacga gcgcaggtag tacagaaagt
     1141 cgagaaacaa ctttttgagc tgtataaaaa cccggagttg aaagttaagc cgaaagaact
     1201 ggaacagcgc ggtggggctt attactctga tgcagcgtgc gaagtgatca acgctatcta
     1261 caacgacaag caagctgaac attacgttaa tatcccgcat catgggcata ttgataatat
     1321 tccggcagac tgggcggtag aaatgacctg taagctgggg cgcgatggcg cgacgccaca
     1381 tccgcgcatt acgcatttcg atgataaagt gatggggctg attcacacca ttaaaggctt
     1441 cgagattgct gccagcaacg ccgcacttag cggagaattt aacgatgtgt tactggcgct
     1501 aaaccttagt ccgttggtgc attccgatcg cgatgctgag ctgctggcac gcgagatgat
     1561 tctggcgcac gagaaatggc tgccaaattt tgccgactgc atcgcagagc ttaaaaaagc
     1621 acattaaccg aggctgatta tggaacgctt actgattgtt aatgccgatg attttggctt
     1681 aagcaaaggt cagaactacg gcattatcga ggcctgtcgc aatgggattg tcacatcgac
     1741 gacggcgctg gtgaatgggc aggctattga ccatgcggtg cagttgagtc gtgatgaacc
     1801 gagtctggcc atagggatgc actttgtcct tactatgggc aagccactga cagctatgcc
     1861 ggggttaacc cgcgatggtg tgctgggaaa atggatctgg cagttggcag aagaagatgc
     1921 tttaccgctg gaagaaatta ctcaggagct tgccagtcag tatttgcgtt tcattgagct
     1981 atttggacgc aaacctacgc atcttgatag ccatcatcat gtgcatatgt tcccgcagat
     2041 tttcccgatt gttgccaggt ttgcggctga agaggggatt gcgttgcgca tcgaccgtca
     2101 gccgctatct aacgctggtg atttaccggc taatctgcgc agttcgcagg gattcagtag
     2161 cgcgttttat ggtgaagaga ttagcgaggc actgttcctg caagtgctgg atgacgcaag
     2221 ccatcggggt gatccttcgc tggaggtgat gtgtcatccg gcgtttatcg ataatactat
     2281 ccgtcagagt gcttactgtt tcccacggtt aacggagctg gaagtgctga catcagcgtc
     2341 gttgaaatat gcaattgctg agcgtggtta tcggttgggt agttatcttg atgtgtaagt
     2401 aaggtggtaa ggtcactccc caggggacgt gaccttatcc atatttacgc cggaattttg
     2461 tcaatcttag gaatgcgtga ccacacgcgg tgttctgcca tcagcgttaa cagttcatcc
     2521 ataaaactac cgtcggtgct gtcagcttcc acaatccctt cttcaccctg gtcagctacc
     2581 ttgattgttg ctttaaactt gcgcgcgtct cccgccagcg caatcggttt aaggtgtttg
     2641 taggcttcca tcagatagta gttggcatcg ccgttgtcag cgatatccgc gatattgccg
     2701 caagggacaa tgaccgcatc gaccgtcagc gaaggtgcac cggcaaaggt agcggctata
     2761 ggcaacaccg taccgtcatc cgcagtcact tcacccattc gggagtagag cagtttggca
     2821 tgaacgcctt tggccttcag ggccttgaga atggcaagaa ggtctgccga tctcacttca
     2881 tcattaagta aaatcgctac cacgcgacct ttcacatcac cgtcaggaat ggcgtacaaa
     2941 cttaaggatg gatccttttt cagaccgttg acgtccggag gtggggtgat attcagctgg
     3001 tcgtcagtca gttcgatacc gagatttttc gccaccgcct gggccagagt gagatcaata
     3061 tgcgccagct ggtcaacaac gtgctcacga atatacggac gaacgacttt gcttaactca
     3121 aaactgaaac catcgacaat atggcgctgc tcgaatggcg tctgacttag ccagaacaga
     3181 cgcggatggg aataatattc gccaaacgat gggctgcgct cgcgaacttt attgccttcc
     3241 acgcgctctt ggtatgattc aaaaccgccg cgtttcggcc ccggtggtgt ttcgcgcggc
     3301 cagttatcgt tgatcgagtt cggttcataa ttcgccgggt tagtgtcgat ccccatacga
     3361 tgcatgccgt cacgctggaa attatggtaa gggcaggtcg ggcggttaat cggaatctca
     3421 tggaaattcg gcccaccaag acgactgatt tgtgtatcgg tataggagaa caaacgtccc
     3481 tgcaacaggg gatcgttggt gaagtccaga ccaggaacaa tatgacctgg atggaatgct
     3541 gcttgttcgt tttcggcaaa gaagttatcc ggattgcgat tgagcaccat tttgccgaca
     3601 cgctgaacgg gcaccagttc ttccgggatc agtttggttg gatcgagaag atcgaagtcg
     3661 aacttgaatt catcttcttc aggaatcaac tggaagccca gttcgtattc cggaaaatcg
     3721 cctgcttcaa tggcttccca caactcgcgg cggtggaagt ccgggtcacg tccggtgagt
     3781 ttttgtgctt catcccaaac gagtgaggct ttacctgcca gtggtttcca gtggaaacgt
     3841 acaaacgttg ccttcccttc ggcattaatc aggcggaagg tgtgaatacc gaagccttcc
     3901 atggtgcggt aactgcgggg gatgccgcga tccgacatcg cccacatcac gttgtgcaga
     3961 gtttcaggtt gcagagaaac ataatcccag aaagtatcgt gggcactttg cccttgtgga
     4021 attgcccagt gcggttctgg ttttaccgca tgaacaaaat cggggaattt atgcgcatcc
     4081 tggataaaga agattggcgt gttattgcca acgaggtcaa aaataccctc ttcagtatag
     4141 aacttggtgg caaaaccacg gatatcacgc acggtatcgg cagagccagc gccaccctga
     4201 acggtagaga acctaacaaa tactggggtg attttgttcg gatctgagag gaaatccgct
     4261 ttggtgatat cgcttaagct tttatatggc tggaaataac cgtgagctgc tgatccgcgt
     4321 gcatgaacaa tacgttccgg gatgcgctca tggtcaaagt gggtgatttt ctcgcgcaga
     4381 ataaaatctt ccagcagcgt tggaccacgg ttaccggcac gcagtgagtt ttgatcatcg
     4441 gcgatgcgca cgccctgatt agtggtcagc gcataatttt cactgccttt gcgtacgtct
     4501 tccagagaat taagtttttc gttacgtgta tcaggggctt tcaggctccc aggggcggta
     4561 ggttgtgcac ctggcggtgt tggttcagcc gctggacgat gagatccgtc ctcaggtgcc
     4621 agtgagtcca tccccggttt cgcttcgctg gaatcgtgta gtggtgactg gtgctgatgt
     4681 gggttctttt cgttatgttg cgacattgaa ctcgtctcct taatttatta ctgaaagggc
     4741 cgctgttttg gtcaaaaaac tctctaacta tagaccacca gccagattcc gcttcggaga
     4801 ctattctgga aacggtgtta tgaaatacac gcaaagcggg acgtacaggg gctaaatcgg
     4861 ctaaactaca gtttttctaa tttttaatat gcgtttagtg aagccagtaa tgaagaaacc
     4921 gctccgccag caaaaccgcc agattattag ctatgtccca cgcacggaac ccgcgccgcc
     4981 agaacatgcg ataaagatgg attcgtttcg tgatgtctgg atgctgcgtg gcaaatatgt
     5041 tgcgtttgta ctgatgggag agtcatttct gcgctcaccg gcgtttactg tgcctgaatc
     5101 tgcccaacgt tgggcaaatc agatccgcca ggaaggggaa gtgactgagt aaggattaca
     5161 tacctcctgg ttttgccggg agttacagga cctgaaattc tgcatttcgt tgctacattc
     5221 attcaaacca cctgtaacaa aaagctgcct ttgacgagct attgctataa ataagtgatc
     5281 ttttttcttt aattgtttat ttattttaaa atggttaagt tttgctaatc atgatgagtt
     5341 atttttgata gttcatcctg tggcgtcgct cttaaactta tattgtaaat ggcttagatt
     5401 taaataagat aataattttg ttaacaatat gaggcgggag atgttttcta caaacttaat
     5461 acagagtaat tatggtgatt taaatattaa aagcctggct ttcgactctt ttaaagaaag
     5521 gcttgagtct accatggcgg cactcacttt ctttatatca acgggtcaat gtgactgcga
     5581 tgaaatagca gagtcgaact tcaatcgtat gattgcctat atgtctaaca ttaactatga
     5641 tgcctcaaag ctaggagcgc cagcacttag ttttgatacg tatctgcagg acaatgttaa
     5701 atatagagtg ataattaata atctttatgg cagtgagttt cggataagag atattaatga
     5761 ggattttgtt gggatggatt ttacctgtga attccgaccc gaaaaaatga cgaatttgat
     5821 caatataaaa aataggttgg ttattcatta tttaaggact atatactatg aaaaaaatta
     5881 tatacatcct gccgggagca tttttgctgc gattcaaaaa aaatgagagt ctcctaaagt
     5941 ttccttcgat aatatcaatg cttaatatca atcttctttt taatccacta aatcttccgg
     6001 gaatgggaag tggtgtgctt gaagatatta tgagtattcc agatagttca cttagaaaac
     6061 gtttcgggta tgaggtttta agcttctcac tacaggcaca ttcattgagc caggaatgta
     6121 ttgataagtt ggacattttt ttcgctgacg atctgtttaa atatgagtca gtatgcatcg
     6181 ccgcgatgga acatttaaaa agtaaagcaa cggcccctat acaaaacgga ccgttgccag
     6241 cataagaatt aacggtgtgc cagttcggcg tcgtcttcgc tatccagaat ggctttatcg
     6301 gtttgcttca gccactggct ggtcagcgtg ccagctgtca tcgagccact aacgtttagc
     6361 gccgtacggc ccatgtcgat aagcggttca acggagatta acagcgccac cagggttact
     6421 ggcaggccca tcgcaggcag cacaatcagt gcggcgaaag ttgcaccacc accgacaccg
     6481 gcaacgcctg cggaactaac ggtaacaata ccgaccagcg tcgcaatcca catcgggtcc
     6541 agcgggttaa tgccaaccgt aggcgcaacc atcaccgcca gcattgccgg atacaaaccg
     6601 gcgcagccgt tctgaccaat ggttgcaccg aaagaggcgg cgaaactggc gatggattca
     6661 ggaacgccca gacgacgcgt ttgtgcttcc acattcagtg ggatagacgc agcactggaa
     6721 cggctggtaa aggcaaacgt cagcacaggc cataccttac ggaagtactt cagcggactc
     6781 acgccattaa tgcccagcag aatgccatgc acagcaaaca taatcagcag accgaggtag
     6841 gacgcgacaa cgaaacttcc cagtttgatg atgtcttgca agttagaacc tgcaaccact
     6901 ttggtcatta gagccagaac gccgtaaggg gtcaactgca tgacaaggcg aaccagtttc
     6961 atcacccagc tttgtagggt atcgatagcg gttaagacgc gttcaccttt cggcgcatca
     7021 tccttcagca gtttcaatgc agctacgccg aggaatgcgg caaaaattac cacgctgata
     7081 attgacgtcg gattggctcc ggtaagatcg gcaaacgggt ttttcgggat aaaggacaag
     7141 accagctgcg gaacgctcag atcagagact ttaccaacat agttactttc aatggcgttc
     7201 agacgtgcag tttctgcacc accctgaacc agaccttcag ccgtcaaacc aaacaggtgg
     7261 tgaccagcac accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccgatggtca
     7321 gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag
     7381 aggcgaagac taacggcata acgatcattt gcagcagttg aacattgccg ttaccaacga
     7441 tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca
     7501 gggcaaggcc aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc
     7561 actgtttatg gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg
     7621 caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca
     7681 gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat
     7741 tgcttattcc agaaaggtga tttttgcctt tttagtattc aacctgatgc ttaataagcc
     7801 gatgaaactg gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgttct
     7861 cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt
     7921 ccagttggtt gcccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc
     7981 agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc aaataaccca
     8041 gcaccattcc ttgtagcgca tcagccggaa tgaaccagcc ttccggaacc ttaaggtaaa
     8101 aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag
     8161 cgcgggcgat cggttttgat atccatttta atcgctgccc aagaaacgac tttgggtgat
     8221 cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct
     8281 gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga
     8341 gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg
     8401 atttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta
     8461 atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg
     8521 gtcattaacc gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg
     8581 ctaatacaaa acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc
     8641 aggctgcttt agaagcgatc atgccattta ccgagtcttc cagcgccacg caggtcagtg
     8701 ggtcaacgcc cagttttgct gcgcagtcga gatatacttg cgaatgcggc ttgctgtaag
     8761 gcagtttttc agccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca
     8821 ctttttccag catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt
     8881 tgcataacgc aacagcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg
     8941 aaatggcacg ggcaataacc cgttctacta cttcctgacg gcttggccca ttccacggtt
     9001 gccgggcgta caaagatcga ccaccatatc gatgcgtaaa cctaaggtgt ccggcagctc
     9061 gttacgacgg gagatatcta cccccaggct tgccatcaca tccagttcgg ctcgatccca
     9121 taaaggttct gagtcgataa gtaatccatc catatcaaaa attgcagcaa gaatctgacg
     9181 cggggttgac attacaacct ctcctttaga gcattatgtg taaggtatta agccgtgctt
     9241 atgattaagc gcctatcata tcgcctttaa agaatgggcg aaaacagata tctacaggta
     9301 aactaagtga aacgacgcgt cttaatcaag gagaactcat gacgtatcaa caagctggac
     9361 gcattgctgt tttgaaaagg attttgggtt gggtgatttt tatacctgca ttgatctcta
     9421 cattgatttc tttgctgaaa tttatgaata cccggcagga aaaccaggaa ggcattaatg
     9481 cggtcatgct cgactttgct catgtcatga tcgatatgat gcaggcgaat acgccttttt
     9541 tgaatctgtt ctggtataac tccccgaccc ctaattttaa tggtggtgtg aacgttatgt
     9601 tctgggtgat tttcatcctg atttttgtcg gactggcgtt gcaggattcc ggtgcccgga
     9661 tgagtcgcca ggcgcgcttc ttgcgggaag gtgttgaaga tcaactcatt ctggaaaaag
     9721 ccaaagggga agaggggcta acgcgtgaac aaattgagtc ccgtattgtt gttccgcacc
     9781 atactatttt cctgcagttt ttctcactat atatcctgcc ggttatttgt attgctgcag
     9841 gttatgtgtt cttttctctg cttgggttta tttaatcaaa aggccgcacg gagcggccat
     9901 tatcctcaac ccatatcatg ctgctaataa tctatccaat gactgctgag caataaccaa
     9961 atgctgaccg ccaaataatc ttgcacgatt taacagcgtg tagagttggt aaaccggttg
    10021 acgttcaagg aaatcggcag gtagcgggga tactgactga tagccgtcat agatttgtgg
    10081 cggctgttca gtatgcagcg gtaacatcgc caggtcgcac tctcggtcac cccagtagca
    10141 ggcagggtcg aaaatgtacg ggccatccgg acccagcgca cagttgccgg accataaatc
    10201 gccgtgcaac agagaaggct gcggttgatg cgaggccaga cgctgctgaa tatgctcgac
    10261 gatggcgtcg atattgccga aagcgatccc tttctctgct gccagttcca actgccagcc
    10321 aatccgttgt tcagcaaaaa acgttgacca gcgacgttgc caggtgttgg gctgtggagt
    10381 tgtggagagc gagttatcaa aatcgaggcc aaattgtggt tggtcactcc attgatgtaa
    10441 acgcgcaatt tgctgaccaa gaataaatgc gctatgcgca tccagcggac gaggtgggag
    10501 ataatccatc accagaaaac tgtagtcacg gtcagcgcct actgcccaaa ccttaggcac
    10561 ggtgacggtt ttactacgcg acagtaactc cagttggtcg gcttcggcgg taaaactggg
    10621 aagcagttcc ctttcatcac atttgacgaa aaagtcatgt cctgcatagc gcaaatgcca
    10681 tgcggcatgg acttctccgc caggcagttc attacgcagt tcgatttcgc cttcacctaa
    10741 ctgctcgctc aaaagacgac tgattgcctg ccacatgatg tctctcccat gttgtctgcc
    10801 agatcataag gttagcgcaa gaatgcggtg taaaaacacg agctaacgca caccacagac
    10861 tgattattgc ccaaaaacca tttattttct gttgctattc cattcctccc aggtcgtgat
    10921 ggtgatttca ggatctttgc cggttgcact ttgggtaagc cccgaaacca gttctctaat
    10981 ttcttcttca ctttgggtac tgataagtcc aaaagtgtta gtccccagct catggatatt
    11041 tccctcatca tccgtcatgg tgagcaaaaa accgtcgcga gtcaggtaat tattcaactc
    11101 gttaatttca gtcaatgtat cctcatgcaa catgacggtt atgacataac ggacaagatc
    11161 gccactggcc atagttcacc cctttgttat cacatgactt ttgagcatag tcggagaaac
    11221 gcgttgccga caggttggtg gtgattcccc caatgctggg ggaatgtttt tgttagcggg
    11281 aaaggtaagc gtaaattttt tgcgtatcgt catgggagca cagacgtgtt ccctgattga
    11341 gtgtggctgc actccccgca gctacgccaa aacggaccat ctcttcaaga gaggcatttt
    11401 ctgccagttt cagtgtcatc gcgccgacca tgctgtcacc agcgccaacg gtactctggc
    11461 ttttcaccgg tggtggcacc acctgaatgc agttttcact atcaacaccc agcgctcctt
    11521 gtggaccaag ggaaacgaca acccgtttgg ccttgccgct attaacgatt tcctgcgcgg
    11581 ctttgcggac atcgtctggc tgggtgagtt cgcgactcac cagcgcactg agttcttttt
    11641 ggttaggctt aaccaactcg atgttaccaa tcgccagtgc tgcacttaac gcttcgccag
    11701 aactgtcgat gatgcagcgg atcccttggt tttgcgcagc ggaaatcagt tgggttaatt
    11761 tttccagctt cacacctggc ggcaggcttc cgcttatgac caggatggcc ccggattcaa
    11821 tttccagaac ttgctcttca agctggcgaa actcatcttc atttaatgcc gcgcctggca
    11881 taacaaaacg atactgttca ccgcttgctt ccacatgtac gtgtaaattc tgccgggtcc
    11941 agtctttggc ttctacagta gcgacgggga cattttcatc cgccaacagt gaaaccaggt
    12001 gttcgccggt cgcgccaccc gccgggaaga tcgctgtggc actgcctcca agatgggcaa
    12061 tggcgcgggc gacgttgatg ccgccgcccc cgggttcgaa caccggtgcg gtacagcgca
    12121 gttttccttc gggataaatt tgcggggtaa ttgttgcgct atcgagagag ggcgcaagtg
    12181 tcaacgtata gatacgtacc atcatttcct cctataggct gatttcagtc tggcaccgaa
    12241 tcagcggaaa gtgaagaaat taacaatatg atttattaga gctttattta aaattttgca
    12301 gataaatata tataaataaa aatctgtttt cgccacatgc ttattcaaaa aatgaaataa
    12361 gaatacattg ctaagttatt cgcgcctttt tataattcgt tacctttcca gggacgctaa
    12421 tctttgatcc ttaagaaagt ttccgggacc gtgatggtct cttttttgtt gtacggactc
    12481 ttgataaatg aagcttttga agacagttcc tgccatcgtt atgttggcgg ggggcatgtt
    12541 tgcgtcactg aatgcagctg ccgatgattc cgtttttact gtcatggacg atcctgccag
    12601 cgctaaaaaa cctttcgaag gtaatctgaa cgcaggatat cttgcacaat caggcaatac
    12661 gaaaagctcc tcacttacgg ctgacaccac catgacctgg tatggacaaa ccactgcctg
    12721 gtcgctgtgg gggaacgcca gtaatacttc ttctaatgat gagcgttctt cagaaaaata
    12781 tgcggcgggc gggcgtagcc gctttaattt aactgattat gactatttat ttggtcaggc
    12841 aagctggctg acagaccgtt ataacggcta tcgagagcgc gatgtgttga ccgcgggtta
    12901 tggtcgtcag tttttgaatg gcccggtaca cagtttccgc tttgaattcg gtccaggtgt
    12961 gcgttacgac aaatataccg acaatgccag tgaaacccag ccgctgggct atgcctccgg
    13021 ggcttatgca tggcagttga ctgacaacgc taaatttacg cagggtgttt cggtctttgg
    13081 cgcggaagac acaacgctga actccgaaag cgcattaaat gttgctatta atgaacattt
    13141 tggactgaag gtggcttaca acgtcacatg gaactctgaa cctccagagt ctgcaccgga
    13201 acataccgac cgtcggacaa ccttatcgct gggctacagc atgtaaaatt atctgccgga
    13261 ccgtgttatg cggtctggct gtcttcgttt gtcactgctc agccccaaat cattgctgcc
    13321 caacgtagca acaacatagc gccacagatt gccagtagca ccaccaccat cttccagtgt
    13381 tttttatcaa agcgtttagt cggcatacca gtgtcctgca tatctcttgt gcgtgataac
    13441 ggagtgtaac aaaagggaca ggcatcaagg gattttttgt tattacagcc gtcgggtggc
    13501 gttgtgcaca tggtcactgt ttctgcgttt tatgaaaaaa ataaaattat gtatcgatat
    13561 atatcgtaaa tagctggtta taaatacgtc tttatttcat cagcactaaa actcaggctt
    13621 tggctgatga catttatagc agcataatat gtttctacag ttttataatg cttatgatga
    13681 atggctatat gaacagctga catgttgtca tgatttttag cggccaatag tgtaaataac
    13741 tgatgttgac taaaagaatg tttttttgca aaagcaccca gcgtcgatat ataagataac
    13801 acgacgtctt cattaccctt gctcatggcg atgtataatg cagggggtcc ctgtgctgta
    13861 gctcctttta ataaatccat gatgttagct ttgctcaact tgtatttaaa ggcgatgcca
    13921 ttaattttag agaggaaccg tgtgacacac aaagggtgat tattttccat tgcggcgtat
    13981 aatcctggct caccatcatg
//
LOCUS       DADLTF010000106        13956 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.106, whole genome shotgun sequence.
ACCESSION   DADLTF010000106 DADLTF010000000
VERSION     DADLTF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.106"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            296..1549
                     /gene="kch"
                     /locus_tag="JN749_002936"
     CDS             296..1549
                     /gene="kch"
                     /locus_tag="JN749_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HAY7369281.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1604..1777)
                     /locus_tag="JN749_002937"
     CDS             complement(1604..1777)
                     /locus_tag="JN749_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HAY7369282.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1920..3380
                     /gene="cls"
                     /locus_tag="JN749_002938"
     CDS             1920..3380
                     /gene="cls"
                     /locus_tag="JN749_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAY7369283.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3415..3744
                     /locus_tag="JN749_002939"
     CDS             3415..3744
                     /locus_tag="JN749_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HAY7369284.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JN749_002940"
     CDS             complement(3797..4801)
                     /gene="oppF"
                     /locus_tag="JN749_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HAY7369285.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JN749_002941"
     CDS             complement(4798..5811)
                     /gene="oppD"
                     /locus_tag="JN749_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HAY7369286.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGAATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JN749_002942"
     CDS             complement(5823..6731)
                     /gene="oppC"
                     /locus_tag="JN749_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HAY7369287.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JN749_002943"
     CDS             complement(6746..7666)
                     /gene="oppB"
                     /locus_tag="JN749_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HAY7369288.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JN749_002944"
     CDS             complement(7752..9383)
                     /gene="oppA"
                     /locus_tag="JN749_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HAY7369289.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JN749_002945"
     CDS             complement(10121..10768)
                     /gene="ychE"
                     /locus_tag="JN749_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HAY7369290.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11245..13920
                     /gene="adhE"
                     /locus_tag="JN749_002946"
                     /gene_synonym="adhC"
     CDS             11245..13920
                     /gene="adhE"
                     /locus_tag="JN749_002946"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAY7369291.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3657 a         3455 c         3248 g         3596 t
ORIGIN      
        1 agatccaata tttaagggca cggtttttgc aaccgtgttc ttgtttggcc tttccttata
       61 gccggtcagt gcattcacta ccacaagcag ataataactt tatctgtatt tctttatatg
      121 atgtgagcat gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt
      181 tgattactgc ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt
      241 tcagaaaatg tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag
      301 tcactgggct acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat
      361 tctcgcgctg gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc
      421 ggtcaatttg cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct
      481 ggcaaacgcc ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact
      541 gttccgggca aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta
      601 caccctgcat ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt
      661 gtttttgctg atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt
      721 tgcatttatt agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag
      781 cgaaggtttt aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac
      841 catgtcaacc gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac
      901 tatttcggtc attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg
      961 tccgcttatc cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg
     1021 taaagatcat tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact
     1081 gaatcaacgc ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca
     1141 acttgagcaa cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc
     1201 agtattaaag aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga
     1261 tgcagataac gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt
     1321 tctcgccgtc agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat
     1381 tctctcaccg caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat
     1441 taataatgat atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa
     1501 cgatgaacaa gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac
     1561 ggaagagtaa acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg
     1621 caaacaataa cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca
     1681 tatttcggcg cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca
     1741 tgcgccagcg ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca
     1801 gaatggtcat tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg
     1861 ccagatgaat ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta
     1921 tgacaaccgt ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg
     1981 caggcgtaac tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc
     2041 tgttgattat ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc
     2101 tccatttagg caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat
     2161 ggcttaacga ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg
     2221 cgccattatt caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc
     2281 tacaactgat gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg
     2341 cgcgccataa tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg
     2401 tggctgaatc attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact
     2461 ccgccgggag tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta
     2521 ttgaagtggt cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc
     2581 tgcgccaaca tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga
     2641 atatggtcga tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga
     2701 tggcgcgtat ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg
     2761 agattgaaac cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg
     2821 aacaggccag tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc
     2881 tcattcacca ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca
     2941 cgccctactt tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg
     3001 gggtggatgt cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca
     3061 gtcgcgcatt ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg
     3121 ggttactgca taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta
     3181 accttgatat gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag
     3241 gttttggtgc agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg
     3301 atgcccgttt atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact
     3361 tcttcagccc gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat
     3421 atggatctaa acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt
     3481 ctcgaactgg ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt
     3541 gaagagcgcg gcggcgcgga gttattcgat ccggcggaag actggcagga acatgttgat
     3601 ttcgatttga acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat
     3661 ggtgaaatca acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt
     3721 tgccatatca tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg
     3781 tcagccctta ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac
     3841 tcccctccag aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac
     3901 ggaaaacaca accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga
     3961 tggttttgtt cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg
     4021 tgtaaggatg tagtggattg tggtagacct catcataggt ccccagttct actgcatggc
     4081 cgagatacat caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa
     4141 taaaaattaa tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg
     4201 cctgaattga cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa
     4261 gaataagagc acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat
     4321 agcggttaat caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc
     4381 gaacttcctg gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc
     4441 cgatggtcat acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac
     4501 tgcgaacggc acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa
     4561 catgaccgtc ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc
     4621 atcccgattc ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat
     4681 cgacggcttt gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa
     4741 agtgcacttt aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca
     4801 taacagttcc tccaccggtt taaagcaagc acgcagacgg ccaggcgtaa actcttccag
     4861 cggcggtgcg ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc
     4921 tttcggtaat cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc
     4981 cgcatcaaga cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata
     5041 aaagacatcg cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt
     5101 gtcacagatc cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa
     5161 ttcccgcttc agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc
     5221 tgtagtgggt tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat
     5281 gactcgctga cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc
     5341 ttccggcatt tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct
     5401 catgttctta tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt
     5461 caacgaagtc attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag
     5521 ttcacgctct ggcaaattga ggatttcacg cccattgaag gttgccgctc cgccaatgcg
     5581 tccgttggca gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga
     5641 ctcacctaca atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc
     5701 cgtgacgtcg ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc
     5761 gtcagcctgt tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact
     5821 ccttaacgat ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa
     5881 aacagtgtca ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc
     5941 gagttcgcgc catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac
     6001 cccaggaagc taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag
     6061 accaccacca caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat
     6121 acaccgccaa cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca
     6181 cgcacaatac gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg
     6241 atgttttggc cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg
     6301 aggatttcca gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc
     6361 gaaccataaa gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt
     6421 gagatacgcc cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca
     6481 aagtagtgac cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca
     6541 taggcaaact gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact
     6601 atcagactgg caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag
     6661 ctgcgccctt cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta
     6721 cttaacatca tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata
     6781 gcacatcgac aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta
     6841 acactaagga atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca
     6901 aaccataaat ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca
     6961 tataggagag cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca
     7021 taggtaaccc tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag
     7081 agccacgggt aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca
     7141 atatcatgaa tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga
     7201 tcgcaaatat catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca
     7261 ttgccagccc catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc
     7321 cagcactaac acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa
     7381 cgggaaaact ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac
     7441 cgaaatcgcc atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat
     7501 taagatgata tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg
     7561 taaaagggct tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata
     7621 gcgtcggaat cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg
     7681 gcctgtgcct tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc
     7741 acgtattgcc attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga
     7801 tctttgccgg tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa
     7861 acaggaacaa tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg
     7921 cgctgcgcct cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc
     7981 ttataatgcg cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt
     8041 tcgttgtagt ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg
     8101 tcgaggaacg ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc
     8161 cacaatgaag aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg
     8221 ttgatggtca acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct
     8281 tcgttacgtt tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca
     8341 gtatacggtg gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca
     8401 atgatatcgc ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc
     8461 ggtttctggt tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg
     8521 tccgggatct cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg
     8581 tcgatttcac cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag
     8641 gttacctggt taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg
     8701 attcgttcgt ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca
     8761 ggctgggtcc atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat
     8821 gggtgaacaa gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg
     8881 tgatcatcaa tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca
     8941 tcaataccgg cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga
     9001 tcaacagaac gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac
     9061 catttcgcat ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat
     9121 tcagcgacgc caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg
     9181 tctcggctga tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact
     9241 tctgaaccat tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca
     9301 tcagctgcca gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa
     9361 cttctcttgg tgatgttggt cattgttttt tggactccct cattataatt actggtatta
     9421 accagcatgt gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag
     9481 gttactttat cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat
     9541 caccttatgt cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc
     9601 tctttaattg ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag
     9661 taccaaatgc ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt
     9721 attgaccgta tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa
     9781 tcaaatagag attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg
     9841 cacaataatt taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt
     9901 actaatatca tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca
     9961 tgatggtgag gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata
    10021 tttttaccta ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga
    10081 agtaaacgga gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga
    10141 aaatcccctt aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca
    10201 taatacgcgt aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca
    10261 tgcggaacaa tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca
    10321 gatagctaat gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc
    10381 ccgccatcaa cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg
    10441 atttttcttg cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta
    10501 tcgtcaccac caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt
    10561 gtagaatcgt gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg
    10621 ctgtaaggtt agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga
    10681 cgggaataat ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa
    10741 cgggaaaatc aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt
    10801 agccataaat aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc
    10861 tcctaactac ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta
    10921 atgtagccac caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat
    10981 gctgaaaggt gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt
    11041 gagtaatctt gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta
    11101 aactctttta gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcatcggtt
    11161 aagcaaatca tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa
    11221 gtttaacatt atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc
    11281 gtagagcgtg taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac
    11341 aaaatcttcc gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg
    11401 gccgttgccg aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct
    11461 tctgaatata tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac
    11521 gacacttttg gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg
    11581 accactaacc cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac
    11641 gccattatct tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc
    11701 gttctgcagg ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa
    11761 ccttctgttg aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg
    11821 actggtggtc cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta
    11881 ggcgcgggta acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca
    11941 tctgtactga tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt
    12001 gttgttgttg actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat
    12061 ctgttgcagg gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg
    12121 aacgcggcta tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta
    12181 ccagaaaaca ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc
    12241 gcacatgaaa aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg
    12301 gtagaaaaag cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac
    12361 actgaccagg ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct
    12421 cgtatcctga ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa
    12481 ctcgcacctt ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac
    12541 gttggtccga aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg
    12601 tggcacaaac ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat
    12661 gaagtgatta ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac
    12721 aatggttatg ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc
    12781 ttcttcgaag tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca
    12841 aactccttca aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg
    12901 aagatcatgt gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc
    12961 tttatggata tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg
    13021 atcgctgtca ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact
    13081 gacgacgcta ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg
    13141 attgtcgacg ccaacctggt tatggacatg ccgaagtccc tgtgtgcttt cggtggtctg
    13201 gacgcagtaa ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat
    13261 ggtcaggctc tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa
    13321 gggtctaaaa atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc
    13381 gcatttgcga acgccttcct gggggtatgt cactcaatgg cgcacaaact gggttcccag
    13441 ttccatattc cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat
    13501 gcgaacgaca acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc
    13561 cgtcgttatg ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct
    13621 gctaagatcg agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg
    13681 aaatctatcc gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg
    13741 tctgaagatg cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc
    13801 gagctgaaac agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact
    13861 gcagcgaaaa aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa
    13921 tcagtagcgc tgtctggcaa tataaacggc cccttc
//
LOCUS       DADLTF010000107        13954 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.107, whole genome shotgun sequence.
ACCESSION   DADLTF010000107 DADLTF010000000
VERSION     DADLTF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.107"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..494)
                     /locus_tag="JN749_002947"
     CDS             complement(<1..494)
                     /locus_tag="JN749_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369292.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JN749_002948"
     CDS             complement(525..1217)
                     /gene="fimC"
                     /locus_tag="JN749_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7369293.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JN749_002949"
     CDS             complement(1418..1960)
                     /gene="fimA"
                     /locus_tag="JN749_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein subunit FimA"
                     /protein_id="HAY7369294.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2441..3307
                     /gene="folD"
                     /locus_tag="JN749_002950"
     CDS             2441..3307
                     /gene="folD"
                     /locus_tag="JN749_002950"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HAY7369295.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3309..3521
                     /gene="ybcJ"
                     /locus_tag="JN749_002951"
     CDS             3309..3521
                     /gene="ybcJ"
                     /locus_tag="JN749_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HAY7369296.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3629..4150
                     /locus_tag="JN749_002952"
     CDS             3629..4150
                     /locus_tag="JN749_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAY7369297.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JN749_002953"
     CDS             complement(4186..5571)
                     /gene="cysS"
                     /locus_tag="JN749_002953"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAY7369298.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5745..6239
                     /gene="ppiB"
                     /locus_tag="JN749_002954"
     CDS             5745..6239
                     /gene="ppiB"
                     /locus_tag="JN749_002954"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HAY7369299.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6242..6964
                     /gene="lpxH"
                     /locus_tag="JN749_002955"
     CDS             6242..6964
                     /gene="lpxH"
                     /locus_tag="JN749_002955"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HAY7369300.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7082..7591
                     /gene="purE"
                     /locus_tag="JN749_002956"
     CDS             7082..7591
                     /gene="purE"
                     /locus_tag="JN749_002956"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAY7369301.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7588..8655
                     /gene="purK"
                     /locus_tag="JN749_002957"
     CDS             7588..8655
                     /gene="purK"
                     /locus_tag="JN749_002957"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAY7369302.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8792..9685)
                     /locus_tag="JN749_002958"
     CDS             complement(8792..9685)
                     /locus_tag="JN749_002958"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAY7369303.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRWVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9682..10329)
                     /locus_tag="JN749_002959"
                     /pseudo
     CDS             complement(9682..10329)
                     /locus_tag="JN749_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10332..11564
                     /gene="allC"
                     /locus_tag="JN749_002960"
     CDS             10332..11564
                     /gene="allC"
                     /locus_tag="JN749_002960"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HAY7369304.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11575..12360
                     /gene="allE"
                     /locus_tag="JN749_002961"
     CDS             11575..12360
                     /gene="allE"
                     /locus_tag="JN749_002961"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HAY7369305.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="JN749_002962"
     CDS             complement(12588..13733)
                     /gene="glxK"
                     /locus_tag="JN749_002962"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HAY7369306.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDALFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(13755..>13954)
                     /locus_tag="JN749_002963"
     CDS             complement(13755..>13954)
                     /locus_tag="JN749_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HAY7369307.1"
                     /translation="QITFTERNIYRLALPLFVGIFLMALPPVYLQDLPLTLRPLLSNG
                     LLVGILLAVLMDNLIPWERIE"
BASE COUNT         3386 a         3680 c         3496 g         3392 t
ORIGIN      
        1 tgagcgctac tcaacaacgc tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa
       61 tcaaagttaa ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc
      121 tgctgggcta actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg
      181 ttaaaacagg gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat
      241 acgatatcct gtgaaccaat gaactcatca ttacgccaga gatccacccg ataaacgccc
      301 gcaggttgat gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct
      361 aataaaaatg ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca
      421 agagatgaat aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta
      481 gtgggtattt tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact
      541 tctctgaccg gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc
      601 actcctgagg gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac
      661 ttttggctgc ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca
      721 ttgatatagt taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct
      781 tgtaattgaa ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca
      841 ttcctgccgt tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac
      901 agagactcac gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa agtattttcg
      961 cttttgggtt cactaacaaa tagtggcggt gtaatgatga atgacttttc ttttacaccg
     1021 ctgctgtttt caatccacga attgaccaga aagagctcat tggtatggct atttttaatc
     1081 cataccgcag tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc
     1141 cctccggcag catgggcgct ggccgaaatg attaaataaa atatcatgag catcaataac
     1201 tttattttag tcatcatagt taatacgcct tgtgtattcc tgacggcaaa agattgcttt
     1261 ggcttcttac aacacatcat tcccttttca tttaataaag cggcatgata caaagtgaac
     1321 gtggtttcga tttacattgc caccataccg cttcagacaa ccggtacatt actttgttat
     1381 aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc ataataaaag
     1441 tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta taacgtgcag
     1501 aaaaatgaag aacgttggtc ccggggatca agttctggtt ggttgagaag ctattcccat
     1561 caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc gttgctgttg
     1621 ttgtatttgt actggatgca atggccaata aattatcatt gattgcatca gcctgaccag
     1681 aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac tgaatggtga
     1741 atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga tattgaccga
     1801 gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc accagttcgc
     1861 cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta gcggcgaata
     1921 gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct tcaatctcat
     1981 ttatcgactc cacatccgta tataaccgat tactttattt aagacactga tagtagtaaa
     2041 attccttttt atcctctaag aatgtcttaa ttgaaaatat gcactctatt ctaaaaaata
     2101 gagagccccg ttagatgaat acttccgcgc aaaatatatt caacacaaat atagacctga
     2161 agcggtaaat taccaggctg aaaattcttt ttatattgtc aggtatttct taaattatct
     2221 taatccttaa acaaggaaat aaatcagttc cagatttaca acgccatcat gtacgaaaaa
     2281 tgaagctttc aatctcagcg acctcagcga cggtgcgcct aaccttcgca agaggtcgct
     2341 tcacgcgata aatctgaaac gaaacctgac agcgcgcccc gcttctgaca aaatagacgc
     2401 atccccttcg acctacgtaa cagatggaat cctctctctg atggcagcaa agattattga
     2461 cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct caaaaagttc aggctcgtat
     2521 tgcagccgga ctgcgggcac caggactggc cgttgtgctg gtgggtagta accctgcatc
     2581 gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa gtcgggttcg tctcccgctc
     2641 ttatgacctc ccggaaacca ccagcgaagc ggagctgctg gagcttatcg atgcgctgaa
     2701 tgccgacaac accatcgatg gcattctggt tcaactgccg ttaccggcgg gtattgataa
     2761 cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg gacggtttcc atccttacaa
     2821 cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc tgcaccccgc gcggtatcgt
     2881 cacgctgctt gagcgttaca acattgatac cttcggcctc aacgccgtgg tgattggcgc
     2941 atcgaatatc gtcggtcgcc caatgagcat ggaactgttg ctcgcaggtt gcaccaccac
     3001 cgtgactcac cgcttcacta aaaatctgcg tcatcacgta gaaaatgccg atctgttgat
     3061 cgttgccgtt ggcaagccag gctttattcc cggtgactgg atcaaagaag gcgcaattgt
     3121 gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt gtgggcgacg tcgtgtttga
     3181 agacgcggct aaacgcgcct catacattac gcctgttccc ggcggcgttg gcccgatgac
     3241 ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt gaatatcatg atccacaggg
     3301 tgagtaacat ggcgacattt tctttaggta aacatccgca cgttgagctg tgcgacttgc
     3361 tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa aatcgcgatt gccgaaggcc
     3421 aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg caaaatcgtc gccggtcaga
     3481 cagtgagttt tgcaggacac agcgtacagg ttgttgcctg atccctttcc cggcctgtat
     3541 caacaggccg gttctctttt cagacttttg ccctctacgc cccgttcatc catgctgata
     3601 gcatcgacat tttgccgaag cgaccatcat gccaaccgtt attacgcacg ccgccgttcc
     3661 cctttgcatt ggtttagggt tgggttcgaa agtcatccct ccgcgtctgt tatttgccgg
     3721 aatcatccta gcgatgctgc ccgatgccga cgtattgtcg tttaaatttg gcgttgctta
     3781 cggcaatgtt tttggtcatc gcgggtttac ccattcgctg gtgtttgcgt ttgttgtccc
     3841 gctcttatgt gtgctgattg gacgtcgatg gttcagggct gggctgattc gctgctggct
     3901 gtttttaacc gtctcgttgc tatcgcacag cttactggat tcggtaacca ctggcggtaa
     3961 gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt ttcgctccct ggcaggtgat
     4021 taaagtcgcg ccgtttgcat tgtctcgtta caccacagcg tacgggcatc aggtgattat
     4081 ttccgaattg atgtgggtgt ggttgccggg gatgctactg atgggaatgt tgtggtggcg
     4141 cagacgataa ccggatgcga aaactcgcat ccggcaattg cgcaattact tacgacgcca
     4201 ggtggtccct tgcgggccat cttccagcac gatccccatc tcgttaagac ggtcacgtgc
     4261 cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc agacgctgtt gaattaacgc
     4321 ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg ctttgcagga acgcttccgg
     4381 ttcttgctcc agcaggccca atacagcgga aagtttacgc aggtgagatg ccattgcatt
     4441 cgccgctgcc atatcttctg ctttcagacg gttcacttca cgcgccatat caaacagcac
     4501 ggaataggct tccggggtgt tgaaatcgtc gtccatcgct tcaataaagc gcgcttcaaa
     4561 cgcttcgcca ccggcaggcg caacggtttt atctgtgccg cgcagcgcag tgtagaggcg
     4621 ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg tagttcagct ggctgcgata
     4681 gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg tagtatttca gcacatcgcg
     4741 cacggtaaag aagttaccca gcgatttgga catcttctcg cggtcaacca tcaccattcc
     4801 ggagtgcatc cagtagttca catactgacc atcatgggca caggtggact gcgcgatttc
     4861 gttctcgtgg tgcgggaaca tcaggtctga accgccgccg tggatatcaa agtggttacc
     4921 cagctgcttg cagttcatgg ccgaacattc aatgtgccag ccaggacgac ccgcgcccca
     4981 cggagacggc cagctcggtt cgccctcttt cgacatcttc cacagaacga agtccatcgg
     5041 gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc tgcagctggt cgagatcctg
     5101 acgcgacagc acgccataag ttggatcggt cgggacgtcg aacatcacgt cgccgttgtc
     5161 cgccacataa gcgtgacctt tggcgatcag ttgttcagtg agttcaataa tttctgcgat
     5221 atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg ttcaaagcat caaaatcttt
     5281 gtgcatttcg gcaatcatgc gatccaccag cgccacaaag ctttcgccat tttcattggc
     5341 gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc acatacttca gtttatagcc
     5401 gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca aaggtacgcc cgtgaccgat
     5461 atgacagaga tcgtaaacgg tgattccaca cacgtacatg ccgacttccc cggcgtgaat
     5521 aggcttaaat tcctcttttt ggcgtgtcag agtattgaag atttttagca tcgaagattc
     5581 cgtttagaca tgtgtgggta attgagttgc gtataatacc catatttccc gcgcgaatca
     5641 gcatacattg cgtgatgatc cgatcgtgcg gttatgctat aacaccaccc tatatatgac
     5701 ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc aaaaatggtt actttccaca
     5761 ccaatcacgg cgatattgtc atcaaaactt ttgacaataa agcacctgaa acagttaaaa
     5821 acttcctgga ctactgccgc gaaggttttt acaacaacac cattttccac cgtgttatca
     5881 acggctttat gattcagggc ggcggttttg aacctggcat gaaacaaaaa gccaccaaag
     5941 aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac ccgtggtacg ctggcaatgg
     6001 cacgtactca ggctccgcac tctgcaactg cacagttctt catcaacgtg gttgataacg
     6061 acttcctgaa cttctctggc gaaagcctgc aaggttgggg ctactgcgtg tttgctgaag
     6121 tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt tgcaaccggt cgtaacggta
     6181 tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag cgtgaccgtt agcgagtaat
     6241 cgtggcgaca ctctttattg cagatcttca tctctgcgtg gaagaaccgg cgatcaccgc
     6301 cggttttctg cgttttttag cgggggaagc ccgcaaggcc gacgcgctgt atattcttgg
     6361 cgatctgttt gaagcatgga ttggcgacga cgatcccaac ccactccatc gccagatggc
     6421 ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt tatttcattc atggcaaccg
     6481 tgattttctg ctcggcaaac gctttgcccg tgaaagcggc atgacattat tgccggaaga
     6541 aaaggtgctc gaactttatg gtcgccgggt gttgattatg catggcgaca cgctgtgcac
     6601 cgatgacgcg ggttatcagg cttttcgcgc caaagtccac aaaccctggc tgcaaatgct
     6661 attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc gcgcgaatgc gcgcgaacag
     6721 caaagaagcc aacagcagta aatcgctggc gatcatggac gttaaccaaa acgctgtggt
     6781 cagtgcgatg gaaaaacatc aggtgcaatg gctaatccac gggcataccc atcgcccggc
     6841 ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc gtggtactgg gtgcctggca
     6901 tacggaaggt tcgatggtga aagtcacggc ggatgacgtt gagctgattc attttccgtt
     6961 ttaaaaaacc cacaactttg ctgatttcac agccacgcaa ccgttttcct tgctctcttt
     7021 ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc accacaggag ttttaagacg
     7081 catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg atggggtcca aaagcgactg
     7141 ggctaccatg cagttcgccg tcgaaatctt cgaaatcctg aatgtcccgc accacgttga
     7201 agtggtttct gctcaccgca cccccgataa actgttcagc ttcgccgaaa gcgccgaaga
     7261 gaacggttat caggtgatta ttgcgggcgc aggcggcgca gcgcacctgc caggcatgat
     7321 tgccgccaaa acgctggtgc cggtgctggg cgtgccagta cagagcgccg cactgagcgg
     7381 tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc attccggtgg gtacgctggc
     7441 gattggtaaa gctggcgcgg caaacgcggc attactggca gcacaaattc tcgcgactca
     7501 cgataaagag ctacaccagc gtctgaatgg ctggcgcaaa gcccagaccg acgaagtgct
     7561 ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt gcgtcctcgg taacgggcag
     7621 ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca ttgctgtctg gccggtcggg
     7681 ctggacgctg aaccggcggc ggtgcctttt caacaaagcg tgattaccgc tgagatcgaa
     7741 cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc atccggcctt tgtgaaccgc
     7801 gatgtgttcc cgattattgc tgaccgtctg actcagaaac agcttttcga taagctccac
     7861 ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg agtggcctgc ggtgtttgat
     7921 cgtttaggtg aactggcgat tgttaagcgt cgcactggtg gctatgacgg tcgcggtcaa
     7981 tggagtttac gcgccaatga aaccgaacag ttaccggcag agtgttacgg tgaatgtatt
     8041 gtcgagcagg gcattaactt ctctggtgaa gtatcgctgg ttggcgcgcg cggctttgat
     8101 ggcagcaccg tgttttatcc gctgacgcat aacctgcatc aggacggtat tttgcgcacc
     8161 agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc aagccgaaga gatgctgtcg
     8221 gcgattatgc aggagctggg ctatgtgggc gtgatggcga tggagtgttt tgtcaccccg
     8281 caaggtctgc tgatcaacga actggctccg cgtgtgcata acagcggtca ctggacacaa
     8341 aacggtgcca gcatcagcca gtttgagctg catctgcggg cgattaccga tctgccgtta
     8401 ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc tgattggtag cgatgtgaat
     8461 tatgactggc tgaaattgcc gctggtgcat ctgcactggt acgacaaaga agtccgtccg
     8521 gggcgtaaag tggggcatct gaatttgacc gacagcgaca catcgcgtct gactgcgacg
     8581 ctggaagcct taatcccgct gctgccgcca gagtatgcca gcggcgtgat ttgggcgcag
     8641 agtaagttca gttaagcagg tgctcatttg attgccggat gcggcgtaaa cgccttatcc
     8701 ggcttaccgc tccggtaccc attgtaggcc tgataagacg cgtcaagcgt cgcatcaggc
     8761 atggtgcacc gattgccgga tgctctcatc actacagcga aatacaggtc cccgcttcgc
     8821 ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca cgcgggttta ctacggcttc
     8881 tgacataacc actcaccgcc gttaccttcg gccccatcga accatcggct ttggcaaatg
     8941 gcgctaactc atccggtgtg gcatggcgaa tggcacgttg ctgcggcgtt ccccagtttt
     9001 catataccgc atcagcatcg gtgaggatca ccagtccatc ggcattaatc tgctcggcga
     9061 gcaacgcagc ggcgagatct ttatcaatca ccgcttcact ccctgcccca tcttccgtca
     9121 caggcacacc gccgccgcca ctgcaaatca ccacatgccc ctctttgagc aacaactcga
     9181 tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc caccacccag cgcaaatatt
     9241 taccatcacg tttcatctgc cagccgtaag ccgcttccag tgcctcttgt tcttctggct
     9301 gataaaccgg accaataaat ttctctggct gcaaaaacgc cggatcatca ggcgaaacct
     9361 caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg ctgtgcgctc aagctctgcg
     9421 ccagcatata gccaatcatc ccctggcttt ccgccaccag cacatccagc ggatacggtt
     9481 ctacctcttt ccacgccaga ttctgtaacg ccagcagccc cacctgcggc ccgttgccgt
     9541 gaacaatcgc cagccgataa gaacgggcca ggcgtgccag cgcgggtaca gcactggcga
     9601 tattgcgata ttgattttcc gccgtcagcg cctcaccgcg ctggagtaag gcgttgcccc
     9661 caagagctac aaccagtgtt ttcatggttt tccttgtaat aattgttggc caagccagaa
     9721 ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc agcagcgaat caatcgcaac
     9781 agcggtacgt ttcgggcaac tcagagcatg gacaaagtgc aggagtggcg aggcgaaata
     9841 tccttgcgcg gcataacgta aataactgac gctcaccgct gtggtaacga gttgaagatt
     9901 gtcggaacag gcaaaaaacg ggcgaccggc gcgcgcatct aaagcgccat aataccaggc
     9961 cgccagcagc attccgctca acgtgtcatc atgacttggc gttaatccgg ggcctttacc
    10021 cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg gcctgaaaac agtgacgaaa
    10081 ctggcgtaat tcagcgggca gcggatcgct tgccgccatc gccagtggcc cgaaaagtcc
    10141 ggtttcctcc gcgcgttgca tccatgcagc tgcaagcggt tgaggatgcg aaggcggcgt
    10201 aatacgcagc aaacaataac gctgtggctg tttaaccgtg aaacgcccga ggcgaatccc
    10261 tttagccaca acctgtggtc gttcattgcc gcataatcca ccgcataacg catcgaattg
    10321 cgtgcggcga aattacacat tcccgtcaag ctatagaaga aacgctgccc tggctttcct
    10381 cttttggcgc tgacccaacg ggtgggatga cccgtttact ttattcgccg gaatggctgg
    10441 aaacccagca gcaatttaaa aaaagaatgg cagcaagtgg gctggaaaca cgtttcgatg
    10501 aagtggggaa tttatacggt cgcctgagtg gcaccgaata tccacaggaa gtggttctga
    10561 gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa ttcggcgcgc
    10621 tggcggcgtg gctggcaatt gactggttga aaacgcaata cggcgcaccg ttacgtacgg
    10681 tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc ttctggggca
    10741 gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt gatgccaaag
    10801 gaaatagttt tgccgatgcg atgaaggctt gcggatttac tctgcctaac gccccactaa
    10861 ctccgcgtca ggatataaaa gcctttgttg aactgcatat tgaacagggc tgtgtgctgg
    10921 aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt cgctatacgg
    10981 tgacgctgaa cggcgagtca aaccatgcag gcaccacgcc gatgggttat cgtcgtgata
    11041 cggtttacgc tttcagtcgc atttgccatc agtcgatcga aaaagcgaaa aagatgggcg
    11101 atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta aatgtggtgc
    11161 cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg ctgcgcgatt
    11221 tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac attggtattg
    11281 atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg gtcgccaccc
    11341 tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt ggtgccgggc
    11401 acgacgcgca aattttcgcg cctcgcgtac caacctgcat gatttttatc cccagcatca
    11461 acgggatcag ccataacccg gcggaacgca ccaatattac tgaccttgcc gaaggggtca
    11521 aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc ataaatggga
    11581 tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc gattgttaaa
    11641 cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat tccgggcttt
    11701 gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt tgttgattat
    11761 ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga aggcattgaa
    11821 acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa aacatttgcc
    11881 ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt tgttaacgcc
    11941 caggccgaag acagccaaat ctttttatat aagcgccgct atgttccggt agaaggccat
    12001 gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta tgaaggtatg
    12061 gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat gaacatgcat
    12121 atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacacgt tcaggaacat
    12181 ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg gatcccggtg
    12241 aaaaaaggcg attacatctt tatgggcgct tattctttac aggctggtta tggcgtaggg
    12301 cgtggtgaag cgttcagcta tatttactcg aaagattgca accgcgacgt agagatttaa
    12361 gtcataccac tcgcctgatt atttttagct atgttggatt ttgccacggt ttataccgtg
    12421 gctttttttg taacattgcc tgatgcgctt cgcttatcag gcctacatga cctctgcaat
    12481 atatagaatt tgcgcgattt tcaggaagga tatagtactc atgccgcatc cggcatgagt
    12541 actgcgctct tatacgaaat aaatcgcatc cgctttaaag gcaagagtca gtttttaatt
    12601 ccctgaccta ttttaatggc gcaggcaata tttcgcgcgc tgttgaagag attggtttca
    12661 ccgctggcga gcacttcggc taaaggtgcc agacgaggca aaatgctgaa tagcgcgtca
    12721 atgccgtact ggtgcaccac ttccacgcca tcacccaata caccagcaat cccaatcacc
    12781 ggtacattaa actgcttcgc caccgacgca acgcccagcg gcgctttacc gcctgccgtt
    12841 tgcgagtcaa tgcgcccttc cccggtgatc accagtgctg ccccctgcac cgcctgcgca
    12901 agattgaccg cattcaacac aatctcaatg cccggtttaa tatccgcatt gagaaatacc
    12961 gccgccgcaa tccccatacc gcccgcagcg ccgccgcccg ccatctgaca gacattaatt
    13021 tcagtttgct gttgtaaaac acgggcgtaa ttttgcagcc cctgttccag ctcttcaacc
    13081 atctccggcg ttgccccttt ttgcgggcca aataccgccg ccgcgccgcg tgcccctacc
    13141 agcgggttat caacgtcaca tgccacttca atatggctgt tcgccagacg cggatcgcat
    13201 tcctccatct caatgcttgc cacgcgcgct aaattgccgc cgttagccgc cagcacctgg
    13261 ccgtcggcat caaggaaacg cacaccaagc gcctgcgcca tgcccatacc gccgtcgacc
    13321 gtcgcactgc cgccaatgcc gagaataata tggcgaatgc cgttatccag cgcatggcga
    13381 attaactccc ccgtaccaaa actggaggcc agcaacggat tacgcttttc aggcgcgacc
    13441 agcatcaggc cacttgctgc cgccatctca atcaccgctg ttttcccgtc tccggtaagg
    13501 ccataaaaag cattcacttt ttcgcccatc ggcccgcaga cttcaagcgt cacgatgttg
    13561 ccacccgtcg cggcgaccat cgcatccacc gtcccttcgc cgccatccgc tatcggcaaa
    13621 cagatatagt gcgcatcggg aaagagggtc gaaaacccgg ctttaattgc ctgacaacat
    13681 ttttctgcac ttaagctctc cttaaaagag tctggcgcaa tgacaatctt cattatcaat
    13741 cctttttcaa caaattattc gatgcgttcc cacggaataa gattatccat aagaacagcc
    13801 agtaaaattc cgaccagtaa gccgttactg agcagaggac gaagcgttaa tggaaggtct
    13861 tgcaaataca caggcggtaa tgccattaaa aatatgccga caaataacgg caatgcgagt
    13921 cgataaatat tgcgctcggt aaacgttatt tgct
//
LOCUS       DADLTF010000108        13814 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.108, whole genome shotgun sequence.
ACCESSION   DADLTF010000108 DADLTF010000000
VERSION     DADLTF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.108"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..454
                     /locus_tag="JN749_002964"
                     /pseudo
     CDS             1..454
                     /locus_tag="JN749_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            514..621
                     /locus_tag="JN749_002965"
     CDS             514..621
                     /locus_tag="JN749_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HAY7369308.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JN749_002966"
     CDS             complement(602..1333)
                     /gene="yehW"
                     /locus_tag="JN749_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HAY7369309.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JN749_002967"
                     /pseudo
     CDS             complement(1338..2264)
                     /gene="yehX"
                     /locus_tag="JN749_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JN749_002968"
                     /pseudo
     CDS             complement(2257..3415)
                     /gene="yehY"
                     /locus_tag="JN749_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JN749_002969"
                     /pseudo
     CDS             complement(3422..4339)
                     /gene="osmF"
                     /locus_tag="JN749_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
     gene            complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JN749_002970"
     CDS             complement(4591..6888)
                     /gene="bglX"
                     /locus_tag="JN749_002970"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HAY7369310.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7084..8799
                     /gene="dld"
                     /locus_tag="JN749_002971"
     CDS             7084..8799
                     /gene="dld"
                     /locus_tag="JN749_002971"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HAY7369311.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JN749_002972"
     CDS             complement(8837..9769)
                     /gene="pbpG"
                     /locus_tag="JN749_002972"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HAY7369312.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9943..10530)
                     /locus_tag="JN749_002973"
     CDS             complement(9943..10530)
                     /locus_tag="JN749_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HAY7369313.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10700..11278
                     /locus_tag="JN749_002974"
     CDS             10700..11278
                     /locus_tag="JN749_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7369314.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11395..12156)
                     /locus_tag="JN749_002975"
                     /pseudo
     CDS             complement(11395..12156)
                     /locus_tag="JN749_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JN749_002976"
     CDS             complement(12209..13735)
                     /gene="mdtQ"
                     /locus_tag="JN749_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HAY7369315.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHRFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQNAPDMQVAEQRIQLAEAQAKAVATQDGPQIDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEVTARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVDGMETDINASKTRQQ
                     LNDVVGKMKIIEARLNALTNHQTKSLKLKPVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIRLTGALGGGYKR
                     "
BASE COUNT         3284 a         3875 c         3491 g         3164 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca gttggcaaga
      421 acaaggccat gatattttcc cactcggtat ttaatgattc gttaacaaat gcgctttact
      481 gtacaatcct ttcgttaaca taaggagtgc attatgcgca tagctaaaat tggggtcatc
      541 gccctgttcc tgtttatggc gttaggcgga attggtggcg tcatgctcgc aggttatacc
      601 tttattttgc gtgctggcta agcgcctgca ccagcctttc aaacaggcgg tctgcgatga
      661 tcgccgccag tgccaccagt aacgccccct ggatcacata cgcggtatta aatccgctaa
      721 gcccgatgat gatgggcgta cccagcgtac tggcccctac cgttgaggcg atcgtcgccg
      781 taccaatgtt gataatcacc gaagttcgca cgcccgccag aatcaccgga gccgccagcg
      841 gtagctcgac cttacgcagt cgctgacctc gactcattcc catacctttc gcaacttctg
      901 tcacgctggg atcaatcgct cccagcccgg caagtgtcac ctgcaggatg ggcagcacac
      961 cgtaaaggat caaggcgata atcgctggtt gcagaccaaa gccgatcacc ggaacggcaa
     1021 tcgccagcac tgcgacaggc ggaaaagtct gtccaacggc ggcaatagtt tccaccagtg
     1081 ggcgaaattc cgcgccccac gggcgagtaa cagcaattcc ggcacctgtg ccaatgatca
     1141 ccgcaaacaa actcgaaatt cccaccagcc agaaatgagc cagtgccaga gctgcaaaac
     1201 tttcttgctg ataaacgggt cgtggcagtt gtgggaacaa ggcagcaaac agcggctggc
     1261 tgtaaggcag ccagaaaatc agcgccacaa aaagagcaat gagccagaac agcggatcgc
     1321 gcaacatctt catacgctta cgcctccacc agcagatcct gaaaatgcag cgtgccgcaa
     1381 ggctggccct gcgtgttcac caccggcagc acctcgcatc cccgcgcgac aaacagggag
     1441 agcgcatcgc gtagcgtcat ctcttctgcc agtgcctcac cttctgcccg ttcttcgcga
     1501 cgcacgtaat ccgccacgct acgtaacgaa agcaggtgca cccccagttc actacgtcca
     1561 aaaaactggc ggacaaaatc attagccgga cgagtcagca tcgtcagcgg attcccctgc
     1621 tgcaccactt caccgtgatc catcaatacc agatgttctg ccagccgtag cgcctcatca
     1681 atatcatgag tgaccagcac aatagtgcgc cccagcaaac ggtgaatgcg cgtcatctct
     1741 tgttgcaacg cgccgcgcgt taccgggtcc agtgcgccaa aaggttcatc catcagtaag
     1801 acttgcggat cggcagccag tgcacgcgcc actcccacac gttgctactg accaccggaa
     1861 agctgatgcg gataacgctc acgcaaattt gactccagcc ccagtagcgc cattaattcg
     1921 tcgatacgat cgtcaatccg cgcccgcgac catttttgta attgcggcac ggtagcgatg
     1981 ttttgcgcca cgctccagtg ggggaacagg ccaatagatt gaatggcata gcccatccgg
     2041 cggcgcaact ccagtactgg cagcgagcga atttcttctc cggcaaagcg gatcactccg
     2101 ctgtcatgct ccaccaggcg gttaatcatt ttcagggtgg tggatttgcc ggagccagat
     2161 gtgccaatca gcaccgaaaa actcccttcc tgaaaattga gattgagatc gttaacggct
     2221 ttttgtgcgc cgaacagttt gctgacatgg ctaaattcaa tcattacgtt tcaccttcag
     2281 cagtgcgata agcaaatcga acagcgcgtc ggtaagcacc gccagaacaa ttaccgggat
     2341 cacccccagc aacactaaat caatggcgct gcttagcagc ccctggaaaa ccagcgcacc
     2401 aaaaccgcct gcgccgatta acgccgcaat caccgccata cctacagttt gcaccatcac
     2461 cacccgcagg ctgcgcagaa ataccggtaa cgccagcggt aactgaacat gcaggaaacg
     2521 ctgcgccccg ctcatcccca tcgctctggc gctctccagc acatcgcgcg ggatctggtt
     2581 caaaccgact accacgccgc gcaccagcgg cagcaaggca tagagcacca gcgcaatcag
     2641 tgcgggtgtc attccggttc ctgctatgcc gagcttcccc agccacggaa aggccgtaac
     2701 cagcgcggca agcggcgcaa tcaacaggcc aaagagcgcc accgaaggca cggtctgaat
     2761 gacattgagc agagaaaaaa ttgccccctg ccgcgcagtg gaaaagtagc accagatgcc
     2821 caacggcaca ccaatcacta acgcaggcag caccgcacca aacagcaacg tcagatgttg
     2881 tgccagcgcg tcgtcaaaca catcctgacg gttggcgtat tctttcattt agtgagagat
     2941 cgttaagcgt gccggagtac agcaaccaca gcggaataat ggcaatctgc atatgcaaca
     3001 accagcgcca cagcggatgc gtggagattc ggcggatggc atcgctacag gccagcaatg
     3061 ccagcgccgc cgccagccag aaaccactgc cgaggctggt acgcgccagc gcactgccat
     3121 tttgcgccag ttgggtcgcc gcctttccag ctccccacac caacaatacg aagacgaatt
     3181 gcgccagaat gagtgcaaaa atgctgcctt ttttagcggg gacaaaacag gctgtcagcc
     3241 aggcgcaacc aacgcccacc agcatccaga tcgtttgcgg ccacagttgc cagagatgaa
     3301 gcccttcacc cgagactaaa cgattaggcg cgtaactgat aaacggcagc gccgctgcga
     3361 ttgccgtcag caacaacagc agcgccagaa caggattaat acgtagataa gtcacgggaa
     3421 attacttcgc ccaccctttt tgtttcaggt agtcggcagc cacttttttg gcatccagtc
     3481 cttccactgc aatgctggca ttcagttgct gcaatgtttt tgcatcgagg ctggcgaaga
     3541 ctggctgtag ccactgtgcc atttgcggat actccttcaa caccgattca cgcaccactg
     3601 gtgcaggcgc gtagataggt tgcacgcctt gcggatcgct taaggtttgc agccccagcg
     3661 ccgcgaccgg agcgtcagtg ccgtaagcca ttgcggcatt aacgccagag gtttgctggg
     3721 cagcggcttt aatcgtcacc gccgtgtcgc caccagccag tgacagcaac tgatcctgac
     3781 cgagcttaaa gccataggct ttttcaaacg cgggtaacgc atcggcgcgt tcgataaact
     3841 ctgccgaggc tgccagtttg aaggtgccgc cctcttgcag ataacgactc aggtcggcaa
     3901 gcgaagtgag tttgtttttc tctgccacat cctgacgcac ggcgatagtc caggtgttat
     3961 ttgcaggcgc gggcgtcagc cagattaact tgttgtgctc cgcatcgagc tttttgactt
     4021 tctcgtagcc ctgcccggca tttttccacg ctgcatcgtt ttcatcttta aagaagaaag
     4081 cgccattgcc ggtatattcc ggatagatat ccagttcacc ggaagtaatc gccccgcgca
     4141 ccacaggagt cgttccgagt tgcactttat tcaccgtcgg tacgccgtgg ctttcgagta
     4201 cctgcaagat gatgttgccg agcagcgcgc cttcggtatc gatttttgaa ccgactttaa
     4261 cgggggaagc cgcctatagc agcaggctca cggctgccaa cataaccagt gaacctgccc
     4321 agagctttaa gagtggcatg atgctttcct cattctttta ctgttgtttt cagcgaatta
     4381 agagaaaagc atagttgata atgacagagt tagcctgagc ggaaggattc agttgcagaa
     4441 tcagataaat acttaagagg catattcggt ccggcgtttc ctgccggatg cggcgcgagc
     4501 gccttatccg gcctacaaag ggcgaaaatt cagcaagtta agatatgaac atcaggcaaa
     4561 gggcgcaaac gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac
     4621 gcgtgcggaa tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat
     4681 ctgttgattc cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc
     4741 gcccggtttc agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga
     4801 agccgtcaca tcctgcaagt acatctgcac taccgtggcc ccctcgcgct taccggtgtt
     4861 cgtcacctgc acgctggccg tcactttgcc gtcacgcttc atggtcggcg cagaaagttt
     4921 cacatcagag acggtgaaag tggtatagct cagaccatag ccgaacggat aaagcgcccc
     4981 gttagcttca tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacggacg
     5041 accggtgttc agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat
     5101 tggcagcttg ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc
     5161 ttcagtcccc gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag
     5221 cgccagcgga cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc
     5281 cgcaatcaag tcacgttggc cttgcggaat agtgatatcg gtccggctgg aggcttcgtg
     5341 cgccatcccc tgtgcttcac cgactacagc caccaccaca tcagattgtt tcgccgtctg
     5401 caccgcttca tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata
     5461 ctgattcagg aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag
     5521 cactttaccg ttttcaccga cggaattttt aatcccggtc agtacggtca cggattgatc
     5581 ggcaacaccg gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc
     5641 aaccaccgca atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa
     5701 cactaggctt tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcggcatt
     5761 ggtatccacc gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaaccc
     5821 catatcatat ttaacgttca gtacatggcg ggcagcatcg tccagctctg ccatggtcac
     5881 tttgccggat ttaatcaacc caggcagata cttcgagtag tactcgtcgc tcatactcat
     5941 gttgattccg gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg
     6001 tttaatcagc tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg
     6061 gtcgcgcaga acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga
     6121 gttcagcgcc accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat
     6181 ataatcatta aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc
     6241 taccgcgccg tatgcggcaa agtgtttgac gctggtcatc accgagtaac gatctgccgg
     6301 gcttttaccc tgcatcgctt ccaccatggt tttacccatt attgaggtga gatacgtatc
     6361 ttcgccaaaa ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg
     6421 tgcccaggtc atattcaggc catcatctgc cgcttcataa gcagagacac gcccgaccgt
     6481 tttcaccgca tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg
     6541 ctgaccgtgc agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat
     6601 cacctgatcc tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac
     6661 ctgaccgtct ttgatcatct cgcggatcgc ctctttcgga ttatccgggc ctacgctgat
     6721 taagcgcagc tgaccaattt tctcatcaac catcattttc ttgagcagat cggtgacgaa
     6781 cgcatcccgc gcttcaggcg ttaatggatg gttaccgaat aaatcatccg ccagtgctgg
     6841 ctgcaggacc agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct
     6901 tcctttgttg cccgacgtgg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat
     6961 gatggagaga gaaaaagaat actcagttta ttgtctgaat tttcaaaata ttcactcgct
     7021 gaattgtcat acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg
     7081 aaaatgtctt ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg
     7141 gtcggtcatt cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc
     7201 cgttctggtc agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg
     7261 cgggtgctga aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca
     7321 ggcctgaccg aaggatcgac gccaaacggt aacgactatg atcgcgatat cgtgatcatc
     7381 agcaccctgc gtctcgacaa gctgcacgtt ctcggcaagg gcgaacaggt gctggcctat
     7441 ccgggcacca cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcat
     7501 tcggtgattg gatcatcgtg tataggcgca tcggtcatcg gcggtatttg taacaactcc
     7561 ggcggctcgc tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata
     7621 gatgaagatg gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg
     7681 gagcagatcc ttagcaagct ggatgacgat cgcatcaaag atgacgatgt gcgtcacgat
     7741 ggtcgtcacg cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg
     7801 cccgcacgtt ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag
     7861 ctggccgtct ttgcggtacg tcttgatacc ttcgaagcgg aaaaaaatca gcaggtgttt
     7921 tatatcggca ccaaccagcc ggaagtgctg accgaaatcc gccgtcatat tctggctaac
     7981 ttcgaaaatc tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa
     8041 aaatacggca aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc
     8101 ttctttaatc tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg
     8161 cattttactg accgtgcaat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg
     8221 cgcatgaaaa actggcgcga taaatacgag catcatctgc tgttaaaaat ggcaggcgat
     8281 ggcgtcggtg aagccaaatc gtggctggtg gattatttca aacaggccga gggcgatttc
     8341 tttgtctgta cgccggagga aggtagcaaa gcgtttttac accgtttcgc cgctgcgggc
     8401 gcagcaattc gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcactggat
     8461 atcgctctgc ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc
     8521 cagctggtgc acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac
     8581 atagtgaaaa aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag
     8641 cagcgcggcg cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag
     8701 acgttgcaga agttctatcg cgagaacgat ccgaccaata gcatgaatcc ggggatcggt
     8761 aaaaccagta aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat
     8821 cgccatccga attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg
     8881 ctttttgttt tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa
     8941 tccaggtacg caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca
     9001 ctaccagcgc caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg
     9061 cattggtaaa gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat
     9121 tagtattgcg gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg
     9181 tagtacttaa ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt
     9241 cacgggcagt tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt
     9301 tcattccgag cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg
     9361 gataatggtg cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca
     9421 gcatatcttt acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct
     9481 ccggcgtctg gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca
     9541 caaccatcgc ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt
     9601 tcgaatagat cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt
     9661 ccggttgtga agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gcaaaaggca
     9721 cagccagcat cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc
     9781 agatagtggt tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc
     9841 gacgccatca taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag taaatgctgc
     9901 attgctgaca tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc
     9961 gtagccccat agtataacgg ttagggtgag cagcacttcc agtaccagca cgccaatcgc
    10021 cagcgtcgaa ctggagaagc taaggccttc ctctttgttg atattcagga agctcggaat
    10081 accaaggtaa agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa
    10141 ccagaccagt ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc
    10201 cgcgaagacc atgcagtgcg caagagacgg acgctgcggg taattgcgcg ccatccacca
    10261 gatgacccgc cccatcaccg caaccccagc cagcataacg ccgtaaaaca agacagccag
    10321 cgccagtccg gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat
    10381 ctgtgtagtg ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg
    10441 ggtgtaatgg tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg
    10501 atgggaaaac agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct
    10561 gacagttcaa gtataagcca gcttgtgatt attttttgtt catccatgta atttgcaccg
    10621 tctgatgtat gtcctttttc agcgtgaatg attcacaggg tgtatgctta caggcaacca
    10681 aagggagaca gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc
    10741 gctggttatc ggtagcctag cggaaggtga aaccgtgact ttgctgggag gcgttgcggc
    10801 acatcagggg ctattaaagt tcccactggt ggtactttct gtggcgcttg gtggcatgat
    10861 tggcgaccag gtactctatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt
    10921 ctcgaaacat caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct
    10981 gtttgtcatt ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg
    11041 tgccagccag ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg
    11101 ggcgttgatt tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca
    11161 caatctcgac cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt
    11221 gggcgtgcgc tggtggctga aacggcgcgg gaagaaaaag ccggataatc aggcgtaaag
    11281 cagttgccac agataagcat tcactccaca tccggcaccg ggtgttccat gcctgatgcg
    11341 acgctggtgt cttatctggc ctaacccatt gaaatatcca cgcacctcac tgcgctattc
    11401 tgggttgaac tgcggattcg ccaacataaa gccgccatcc actatcaacg actgccctgt
    11461 cgtgtaattt gcgccctccg aacaaagcca caccaccagg ctggcaatct catgcgttgc
    11521 gccaaaacgc cgtagaggaa tcgaaggctc cgcgtcgggc ttcacgtcgc tatcatccat
    11581 gctgttcatt ggcgtggcta tcgccccagg cgcgactgcg ttcaccaaaa tcttatgcct
    11641 gaccaactcc agcgccatcg ttttggttaa cccgccgagc gcatgtttag cggctgtgta
    11701 ggcgctggca tccggcagcg gcgtatgttc atgtaccgac gtaatgttga tgatgcgacc
    11761 gccctgccct tatttcacca tctgacgagc cgcaatttgc gagcataaaa atgcaccatc
    11821 aacatcaacg gtaaaaatct tgcgccactc atcaaaagcc atatcaagaa acggcgcttt
    11881 ggtcattgca cccgcattat tcaccagcac atcaatacgc ccaaatcgct ggatgagtgt
    11941 ctccagcgcc tgcgcacctt ctggcagatt gccgagatcc agttgcacga tctccgcacg
    12001 tacgccgtgg gtaaccacct cacgcgcggt atcttttgcc ccttcttcat ctgagtgcca
    12061 ggtaatgcca atatcaaacc cctgatgcgc cagtaataac gcgcactctt tgccgatccc
    12121 cgaatcggaa gcggtaatta tcgcgaccta tgccatcgag ttctccactt aacgctgaat
    12181 aaacgttaag tatagaaggc gcatatcatc agcgtttgta acccccgccc agcgcaccgg
    12241 tgagtcgaat ggaggcatcc agccactgcc cttgcaataa caggccattg gcccgctcac
    12301 gcagcgccgg gattctggct tcgctgacgc gggaaccagc aatgatgccc gcgttaaagc
    12361 gggcctgcgc aagacctacc acacgcaagg catcgcgctc aatttgcgcc tgatgctggt
    12421 ttttctccgc cagtgtctga acctgactgg ctgcccgcgc cacgtcattc actgcttcaa
    12481 ccaccgcttt gttgtagctg gcgatagaca agttgctttc ggcttttgcg atatcgagat
    12541 tggcgttaag acgaccacta tcgaaaatag gtagcgtcag gcctgccgta acgcccattt
    12601 gctgcgcgga atgacggaac agatcgctta agtgcaacgc atcctgttgc aggaaggcca
    12661 tcaggttgat gtcaggataa aatgccgctt ttgccgcatc aatagtgctt aacgatgact
    12721 caacgtacca gtgcgccgcc tgcaaatctg cccgccgggc cagtaaggag taccccagtt
    12781 catcaggaag ctggcttgcc actttcggca acgcgaccgg tttaagcttc aatgacttag
    12841 tctggtgatt tgtgagtgca tttaaccgtg cctcaataat tttcattttc cccacgacat
    12901 cgttgagctg ctgccgggtt ttgctggcat taatatcggt ttccataccg tcaactgaag
    12961 aagtaatccc gttctgatat agctggcgat cggtcgcgat aatggtgttc tgctcttttt
    13021 ctatttgctg caagaccgtg tttaacgccg cctgggtttg ccactcccag tacaggcggg
    13081 ctacgctgcc agccagcaat tggcgggttt gctcgcgttc cgccgcccgt gctttaaccg
    13141 tacccaggcg ggcagtaacc tccgcccgat tctttcccca gatatcgaga tgccagcccg
    13201 ccgttaagcc aaaagttccg ttggtgtacc acggaccggt cgtacctgcg gccggatcgt
    13261 tcagagcaaa cggccccatt agcccttctg ccgacatttt ttgccgctcc atatccgccg
    13321 aaaagtcgat ctgcggacca tcctgagtgg caactgcctt cgcctgggct tcagctagct
    13381 gaatgcgctg ttcagccacc tgcatatccg gtgcgttctg tagtgcattg ttaattaatg
    13441 aagtgagctg attatcgtga tactccagcc accattggct gtctggccaa ccatttttca
    13501 gcgccgtggg taatgcggtg tcaacttgtg cagcgggcgt ttgctggctt aacgcctggc
    13561 gggtttcatg cataggcgca cacccggcca gcatcagtaa cagcggaaaa caggcgatgg
    13621 ctggataaaa ggaatcacga ttcatggggg aataatcagg taagaaacgg tgcgcggaga
    13681 ttaccgtgtg ttgcgatata ttttttagtt tcgcgtggca atacatcagt agcaataaaa
    13741 cgacataccc agaaaaatat agactaagta aatgatatct tccgatttat cttaatcgtt
    13801 tatggataac ggca
//
LOCUS       DADLTF010000109        13544 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.109, whole genome shotgun sequence.
ACCESSION   DADLTF010000109 DADLTF010000000
VERSION     DADLTF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.109"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..892)
                     /locus_tag="JN749_002977"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="JN749_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            1133..1933
                     /locus_tag="JN749_002978"
     CDS             1133..1933
                     /locus_tag="JN749_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAY7369316.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2011..2781
                     /locus_tag="JN749_002979"
     CDS             2011..2781
                     /locus_tag="JN749_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7369317.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="JN749_002980"
     CDS             complement(2829..4187)
                     /gene="mltD"
                     /locus_tag="JN749_002980"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HAY7369318.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="JN749_002981"
     CDS             complement(4259..5014)
                     /gene="gloB"
                     /locus_tag="JN749_002981"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HAY7369319.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5048..5770
                     /locus_tag="JN749_002982"
     CDS             5048..5770
                     /locus_tag="JN749_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7369320.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="JN749_002983"
     CDS             complement(5767..6234)
                     /gene="rnhA"
                     /locus_tag="JN749_002983"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HAY7369321.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6299..7030
                     /gene="dnaQ"
                     /locus_tag="JN749_002984"
     CDS             6299..7030
                     /gene="dnaQ"
                     /locus_tag="JN749_002984"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAY7369322.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7163..7239
                     /locus_tag="JN749_002985"
     tRNA            7163..7239
                     /locus_tag="JN749_002985"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7197..7199,aa:Asp,seq:gtc)
     gene            complement(7370..8416)
                     /locus_tag="JN749_002986"
     CDS             complement(7370..8416)
                     /locus_tag="JN749_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HAY7369323.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8427..9302)
                     /gene="tssG"
                     /locus_tag="JN749_002987"
     CDS             complement(8427..9302)
                     /gene="tssG"
                     /locus_tag="JN749_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HAY7369324.1"
                     /translation="MEKFRLGQTPALTFAPREIASVGWQEGRLHISLYSLGLWGPNGP
                     LPLHYTELARNRTESRRDPTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRF
                     AFYVASLSGQDLRESADSPLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQE
                     YVLHWIAVADDEITRLGMPAPSSVIGNSALIGQAVPDMQYKFRLVIGPLTLEDYRRFL
                     PGSNNLPVLTELVRAFSGYEYCWEIELQLKPHAAPPAVTGGPYQLGWTAWAGKAMHDN
                     PVTGMIFEPEHYLAH"
     gene            complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="JN749_002988"
     CDS             complement(9419..11302)
                     /gene="tssF"
                     /locus_tag="JN749_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HAY7369325.1"
                     /translation="MDPRLLGYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRPLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAT
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="JN749_002989"
     CDS             complement(11318..11812)
                     /gene="tssE"
                     /locus_tag="JN749_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HAY7369326.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11809..12639)
                     /locus_tag="JN749_002990"
     CDS             complement(11809..12639)
                     /locus_tag="JN749_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HAY7369327.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="JN749_002991"
                     /pseudo
     CDS             complement(12626..13540)
                     /gene="tagK"
                     /locus_tag="JN749_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3102 a         3521 c         3617 g         3304 t
ORIGIN      
        1 ggagctgttt ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc
       61 gcacggtcgc tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc
      121 attaactcca ccaattatcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac
      181 gcaatcccgt tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa
      241 ccgtacttca cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa
      301 gcgggttcag tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc
      361 ccgtagcggg aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc
      421 ctggcacgta agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt
      481 tcgatcaaat taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt
      541 tcacggaaag gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg
      601 atccgtaaca gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc
      661 atctcctgca aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta
      721 agttgccgcg tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc
      781 gcccggctta ccgctgagtt tgcttgccct aaaattgttc cgctgcccgg ctaaagctgc
      841 cgctttcgac gaccgaaaca aaaatggcga gttcttccga cgtggctttc atttttgctc
      901 ctgttgcaaa atagatgaga tattttgaat ttatttgtca ttaaaccatc aggatgcgtg
      961 atatgtcatc cgatttaatg ttctcaataa tgagcaaatt tctgaccggt gtaagcactt
     1021 gcttacataa caatatacaa ttgctcgttg aaagagtgag ctaaaatccc tataacagta
     1081 gaaccctccc gagtgcggaa gggttgacgt aatagaggtt tcaaagtcaa aagtgcgaaa
     1141 aaacacctat gccatgcgct atgttgccgg acaacctgcg gaaaggatct taccgccggg
     1201 gtcttttgcg agcatcggcc aggcattacc acctggagaa ccgttaagta ccgaagagcg
     1261 tattcggatc ctggtgtgga acatatacaa acagcaacgc gctgaatggt tgtcggtatt
     1321 aaagaactac ggcaaagatg cacatctggt gttattgcag gaagcgcaga caacgccaga
     1381 gttagtacag tttgcgaccg ctaactatct tgccgccgat caggtgcccg ctttcgtgct
     1441 gccacaacat ccttccggtg taatgaccct ttcggcggcg catccagtgt attgctgccc
     1501 gttacgtgaa cgagaaccca ttttgcgtct ggcgaagtcg gcactggtga cggtctatcc
     1561 attacctgac acccgcctgt tgatggtggt taatatacac gccgtcaact tcagtctggg
     1621 cgtggatgtc tatagtaagc agttacttcc tattggtgat cagatagctc accacagcgg
     1681 cccggtcatt atggcgggag atttcaatgc ctggagccgt agaaggatga acgcgttata
     1741 tcgctttgcg cgggaaatgt cgctgcgcca ggtgcgtttt accgatgatc agcgccgtcg
     1801 ggcgtttggc cgcccgctcg attttgtttt ctaccgtggt ctgaacgtca gcgaagcttc
     1861 tgtattggtt acgcgcgctt ccgatcacaa tccgctactc gttgaattca gtcccggcaa
     1921 gcctgataaa taaggtatgt caggtctgcc acagggcaga ccaacgtttg gcgctgtcct
     1981 tttatactca aacaacaaaa ggacagcgca atgacaacac aatcccacca tgaccacgta
     2041 gaaaagcagt ttagctctca agcctgcgaa tatctgacca gtaccgtaca tgcatccgga
     2101 cgagatttac agcgcctggc ggtgcgtctg gctgattatc ctggtgcaag tgtacttgat
     2161 atgggctgcg gagcagggca tgccagcttt gttgctgcgc aaaacgtgag cgcggtggtg
     2221 gcgtatgact tatctgccca aatgctggat gtcgtggcac aagctgccga agcccggcaa
     2281 ctgaaaaata tcaccacccg ccagggatat gctgaaagtc tgccatttgc cgataacgca
     2341 tttgatattg ttatcagccg ttattctgcc catcactggc atgatgttgg tgcagcactg
     2401 cgtgaagtga accggatatt gaaacctggc ggtaggatga ttgtgatgga cgtaatgtct
     2461 ccgggtcatc cagtgcgcga catctggtta cagacggtag aagcattacg cgatacctct
     2521 cacgtacgaa actacgccag cggtgagtgg ttgaggttaa tcaatgaagc caatctgata
     2581 gttgataatt taattacaga taagttaccg ctggaatttt gttcatgggt cgcgagaatg
     2641 cgtacgccag aagcgttagt agacgctatt cgcatttacc aacagagcgc atcgacagag
     2701 gtgagaacgt attttgcctt gcagaatgac ggctctttca ccagtgatat catcatggta
     2761 gaagcacata aagcggcata aataaaaaag gcaccggggg aatcggtacc tttttattat
     2821 ctggtttgtc aggaatctgg catgttgttg tttttcacaa acaacgtcag cttgtcgcct
     2881 ggttgcagat tcgcagtgtc gctgttccag cgcatcacat ctttgatgtt cacgccgtgg
     2941 cgtttagcaa tgcttgaaag cgaatcgcct ttgcgcacac gatacgtaat gctatcgctg
     3001 ttgtttgcca gtcgctgtgc gctactgcct gcaccaatcg tcaaactttg gcctggcttc
     3061 agcttagatc cgcgcagttt gttccactgc tgcaaatctt tggtgcttac gccgagacgt
     3121 gaagcgatgc ttgaaagcgt gtcgccagag cgtacggtgt aaacacggct gttaagcggc
     3181 gtattgtcgg caaccagcgt cgactgtaca gcagcaattt cgcctgaagc gagagattca
     3241 cgcagttgat ctgcatgctt ctttggcacc atcacgtact gcggcccact tgcgcccagc
     3301 gtggagcctt tcacgccagc gttgaatgtc ttcagcttgc tgacggaaat ccccgccata
     3361 tctgcaacct tcgccatttc aaccgggcta ctcaggtgca cacgcgccag agcacggctt
     3421 tcatcggtcg ttggcagacg tacgccataa cgcttgctgt ttttgagaat atcactcaat
     3481 gccagcattt taggcacgta ctgcttcgtt tcctgcggca acggtaacga ccagaagtcc
     3541 gtggatttcc cacgcgcttt gttcgtttta attgccttca tgacccgacc ttcgccgctg
     3601 ttataagccg ctacggtcag aagccagtcg ccgtcaaaca ttttgttcag acgctgcatc
     3661 atattcagcg cggcagttgt tgaagcaaca acatcgcgac gcgcgtcata attgcgggtc
     3721 tgtttcaaac cataattgcg ccccgtgctc ggaatgatct gccagatgcc tgcggcattg
     3781 gcgccagacg ttgcgtgagg atcaaaagcg ctctccacta tgggtagtag taccagttcc
     3841 ataggcatgt tacgtttttt aacttgccct gctatccagt acatatacgg ctctgcccgt
     3901 aaagttacat cgtggagata gctcttattg cgtaaatatt tctgtttctg ttcgcgaatc
     3961 cggtcatttt ccggaattcc catctttagc tcgtcgccaa tgaaagccca caagtcacca
     4021 tctggcgcga tagacgtccc atcgtccatc catcgtgcct gacttgtaaa ctttgctgct
     4081 tccccttgac cagctgcaga aaggctctgt gcgtgctgtt gaacgttgcc ggtactctgg
     4141 caacccacga gcaggacaga ggcgagtaat atcgcttttg ccttcatgtg tgtgtcaata
     4201 gttgcttaaa agacgaccga tcataacggc gaacggagcc gatgacaaga aagttttatc
     4261 agaacctatc tttctttgac cttaaccatg caaaacgctc ttcaggttgt tgcaataatg
     4321 tttcttcatt aattacatta attaaatcaa tatcttccgt tcttaaaaaa acattaattt
     4381 gccgctcatt tttcagaatt acgggtagtg ttatttgatt ttttgcccgt aactccttaa
     4441 ctttacgata ataatcattt atggacaaat cgtgcggaaa aatactcaaa gcaaacttca
     4501 tatttgataa cgtatattca tgagcacaac ataccaatgt atcgtcaggt aacgcactta
     4561 gctttttaag tgattgatac atttgtaaag ctgtcccttc aaacaaccga ccacacccac
     4621 cagaaaacag cgtgtcgccg caaaatagat aaggtttact gaagtaacag atatgtccta
     4681 aagtgtgacc cggtgtggta attacactaa attcatgccc taaaacgaag gcagtttcgc
     4741 catctttgac tacctgtgtt gttcccttat cttgtgtctc ttgtggacca tacaccacaa
     4801 tttgcggaaa cttttccacc agttctttta cgccgccaac gtgatcgtgg tgatggtggg
     4861 ttagaaatat ggcctccggt tgccagttat tggcggtaat ggcgtttaat actggctctg
     4921 cgtctccggg atcgacaatc aggcagcgac ctgcctcatc attcaaaacc cagatgtaat
     4981 tgtcatcaaa ggcgggaata ctgttaagat tcatagatta cctctcagtg taaaacggaa
     5041 ggttgtgatg aaaccggcaa gagtccctca aactgtcgtg gctcctgatt gctggggcga
     5101 tttgccctgg ggagagcttt atcgcaaggc gctggagcgc cagctcaacc cgtggttcac
     5161 taaaatgtat ggttttcatc tgcttaagat tggcaattta agcgcagaaa tcaattgcga
     5221 agcgtgcgcg gtttctcatc aagtgaatgt ttctgcgcaa ggaatgcccg tccaggtgca
     5281 ggcggatcca cttcatcttc cttttgccga taaatccgtt gatatttgtc tactggcaca
     5341 taccttgccg tggtgcaccg atccgcatcg tttattacgt gaagccgatc gggtactgat
     5401 tgatgatggc tggctggtga ttagtggctt caaccccatc agtttaatgg gattacgcaa
     5461 acttgtgccg gtattgcgca aaacctcgcc ctataatagc cggatgttta ctctgatgcg
     5521 gcaactggac tggctctctt tgttgaattt tgaagtgctg cacgccagcc gtttccacgt
     5581 tctcccgtgg aacaaacacg gaggaaaact attgaatgcg catattcctg cgcttggttg
     5641 cttacaactt attgttgccc ggaaacggac tattccttta acgctaaatc cgatgaaaca
     5701 gagtaaaaac aagccacgaa ttcgccaggc ggttggagcc acccggcaat gtcgtaaacc
     5761 acaggcttaa acttcgactt ggtagcccgt atcttccagt gtgggattca tcgccgcggc
     5821 acgagccagt tcatcacagc gttcgttttc cgggtgtccg gcatggcctt taacccattc
     5881 ccatttgatt tgatgctgcc ccaatgcagc atcaagacgt tgccagagat cgacattttt
     5941 tactggtttt ttgtctgctg ttttccagcc acgttttttc cagttatgga tccactgggt
     6001 gataccctgg cggacatact ggctgtcggt actcaaaatg acttcgcaat gttcttttaa
     6061 cgcctccagc gcgacaatag cggccatcaa ctccatacgg ttgttggtgg tgcgggtgta
     6121 gcctgcgcta aaggttttct cgcgtccgcg atagcgtaaa atagcgccgt aacccccagg
     6181 tccaggatta cccagacacg aaccatcggt gaaaatttct acctgtttaa gcatctctgg
     6241 tagacttcct gtaattgaat cgaactgtaa aacgacaagt ctgacataaa tgaccgctat
     6301 gagcactgca attacacgcc agatcgttct cgataccgaa accaccggta tgaaccagat
     6361 tggtgcacac tatgaaggcc acaagatcat tgagattggt gctgttgaag tagtgaaccg
     6421 tcgcctgacg ggcaataact tccatgttta tctcaaaccc gatcggctgg tggatccgga
     6481 agcctttggc gtacatggta ttgctgatga atttttgctc gataagccca cgtttgccga
     6541 agtagccgat gagtttatgg actatatccg cggcgcggag ttggtgatcc ataacgcagc
     6601 gttcgatata ggctttatgg actacgagtt ttcgttgctt aagcgcgata ttccgaagac
     6661 caataccttc tgtaaggtca ccgatagcct tgcggtggcg aggaaaatgt ttcccggtaa
     6721 acgcaacagc ctcgatgcgc tatgtgcccg ctacgaaata gataacagta aacgaacgct
     6781 gcacggggca ttactcgatg cccagatcct tgcggaagtt tatctggcga tgaccggtgg
     6841 tcaaacgtcg atggcttttg cgatggaagg agagacacag cagcaacaag gtgaagcaac
     6901 aattcagcgc attgtacgtc aggcaagtaa gttacgcgtt gtttttgcga cagatgaaga
     6961 gctggcagca catgaagccc gtctcgatct ggtgcagaag aaaggcggaa gctgcctctg
     7021 gcgggcataa atacatgtaa aaggcgctaa aaatagcgac ttgggcgatt tttgcagcaa
     7081 acgattcaaa agatgagaaa aatcgttgac gaaggtcgag gcaatccgta atattcgcct
     7141 cgttcccaac ggaacacaac gcggagcggt agttcagtcg gttagaatac ctgcctgtca
     7201 cgcagggggt cgcgggttcg agtcccgtcc gttccgccaa tattcagaaa gcccgaatca
     7261 gtaatgattc gggctttcgt cgttttggtg aagtgagaat ttttcactcc gaagtaaaac
     7321 ggatatgatt taaaaaatgg ccagacatta taaattctgg ctacttctgt tactccagcg
     7381 catcccattt ctccagcagt tctactggaa tttcatttat tacctccgag acgcgttttc
     7441 cgaccaggcg ttctgcctgg cgcagcaaag gaatggtcgg gctgctcggt tcgctgtgct
     7501 caaaccagcg acggatgaca cgcaggcgtt ccagcgcgtc gtttcggtcg cggatctgcc
     7561 ggggttctgc gccggtcatc ggtgatattt cctccgcgat atgcaccggt agcgcaatct
     7621 gctctgcctg catctgttca gtatgaacga tggtggcgtt ttccggctgc tcagcctgtg
     7681 gttgtggcag gatctccggc gtggtgctct gcactgctcc cggtaacagg gccagcagtt
     7741 gcgtcagacg ggagaagtcc ggtgcctggt cattcagcgt ttcacgcgca tggcgttgca
     7801 gccgctcagt ggtctctgct gcctggcgaa aggcatccag cggcagtgtg ccgcgcgctt
     7861 ccaggtctgc cagttgctgg cgtaccgatt ctggtgccag cgcatccgcc gggcgtaatg
     7921 cagatagcga acgctccaca tcccgcacct gaaggcgcat ggcagcatta tttgacagtg
     7981 taatgccacg aatatccgcc attacacctt cgtggtccag caacgccgcc agcgcgttac
     8041 tgcgtgccac cgcagcatct tcggcggtga tatcttcagt cgcgagcagt tgaggatgga
     8101 tggcgtccgg gtacgtggag cacaacgttt ccagcagcgt gagcgcctcc gacagtcccc
     8161 tggcaccggc ctgctggata cggcagcgca gcaggacaac cagaatgcgg atatctttgc
     8221 tgcgcatcag cagacgatgg gcgtcccgct caagctccgc ccagttaatg ttttccggcg
     8281 tactgacaaa atcgccatat tgcgcttctt cacgcggtgc ggcgcgggtg aacagcagca
     8341 ggtattccgg atcgtactcc agatccgggc cgcaggggca ttccgccgaa attgggtcag
     8401 acaggaatat ttccatttaa ttatcctcag tgcgccaggt agtgttctgg ttcgaaaatc
     8461 atgcccgtca ccgggttgtc gtgcatggct ttacccgccc atgcagtcca acccagttgg
     8521 tacggaccac cggtaacagc cggaggggcg gcgtggggct ttagctgtag ctcaatttcc
     8581 cagcagtatt cataaccact aaaggcgcgg accagttctg tcaggacggg caggttattg
     8641 ctgccaggca gaaaacgtcg gtaatcctcc agcgtcagcg ggccgataac cagccggaac
     8701 ttatactgca tgtccggcac ggcctggccg atgagtgcac tattgccaat aacggaagag
     8761 ggggcaggca tccccagccg ggtgatctca tcgtccgcca cggctatcca gtgcaggaca
     8821 tactcctgta tccggaaagg tacaccgaaa tagtgcgcca gcgtggtggc cagaccgtcc
     8881 ggattgcggg actcacgcac cagatgggcc gatgccgcca gacgagcgtg atctggcaac
     8941 gggctgtcag cgctctcccg caaatcctgc ccgctgaggc tggcgacata aaaggcaaag
     9001 cggtcatgtt gcggtttatc cagaccgccc ccagcggact gggcgctgcg ccatgcctgg
     9061 taaaactgcg tcagccagcg gtgatgaaaa agatcagaaa aacgggtcag cgtcggatcg
     9121 cgtcggcttt cagtccggtt acgcgccagt tcggtgtagt gcagcggcag tggaccgttc
     9181 ggcccccaca gcccgaggct gtacagactg atgtgcaggc gcccttcctg ccagcccaca
     9241 ctggcgattt ctcgtggcgc aaaggtcagc gcgggcgttt gtccgaggcg aaatttttcc
     9301 atccgtggtt gctaggtgtt gcccgccgga acggtacaca actgcacatc cacgcggcgc
     9361 atcaggttca gaaaaccgta atgccagggg ggttcctgcg cctgagtgaa atcgttgctc
     9421 atacgcttcc ccgctgtccc gccctgacag gccaggtcat aattttgccg cgctgcatgc
     9481 tgtgcagcgt catctgcgag aaggtattaa tggaaacatg gcgggcgata tagtgttcca
     9541 gcaccaggcc gaacagatac ggactgatgc cggaaaaacc ttcttcatcc accgtcagtt
     9601 cacaactgac gccacgccca tagaccagta gtccggaacc gggcagacgg cgggtgaccg
     9661 gggtcgtttt acaaccaatc aggctgcgca cctggcgcga ttgcggactg tcatgagcag
     9721 gaataaacag gttcagcaga tctcgcaacg cctggccgcc ggtacggtga tccagatcgg
     9781 ccagcggcag ataattaaaa gataactggc ggatcaaccg ccatgccatc tcacgttcag
     9841 ccagcggggg ctgcggcgag cgtggcgggc ggataagccc tacgcccgcc accgggatgg
     9901 ctgcatccac ggttagatcg tcccggccat tgcgggcaat aagacagggc agatcgcggt
     9961 tagtgaccat ggccgtgatg gtgatgtggc gcagattttc cgggtacggc gcttcatact
    10021 ggtcaaccag cgacaggaac acttccgagc ccgtataggg ggtacgggtg ccgtaacggc
    10081 gggcgttttc tgataagcgg cggggctccc ggcgcaggga aaaataacgt ccgtgattgc
    10141 cttcatcatt attgcgggtg tggtagagcg ggcgaaaggc catcttccgt gtggtgtcgg
    10201 tttccagccc ttccacttcc tggaccgaga acacctcata atccagcgga tgggttctgt
    10261 ccaccaccag atgctgttcc gtcgtgctgt gcgtgacatc gatccgggcg gtgacgcggg
    10321 gaaacaggtt gataaccggg gtacagaaca ggctgaactg tgccgcattt gtctggtgga
    10381 tcagccagtc cggcggcagg cggttcagca ggataacaat ctccgccacg ccaccgtcaa
    10441 ttttctgtaa tccggcagac aggcctgtcg gggtgaaaaa atagaagcgt tccggacagg
    10501 caaaatattc atgcagcagg ttatgaccgt gaaacacgtt ccacgccagc ggcagcagcc
    10561 cctgatccgg ctccaggcct tcgtacataa cgggctgttg cagattcaca tccagcgcgc
    10621 cgtcaaaatg ccccggaatg ccagccaggg tcgcgatggc gctggtatgt agaagttcta
    10681 acaggtgtga ggccgtacgc tcctcgccgc agagataaaa cggcagcctg tccagtccgg
    10741 caagctgact gaacgttagt tcaccaaagg tgcgcagggt aatacgcagc gccccggcga
    10801 cgtgaatgtt ggccggcagg tagcggtgca gggcaggcat atccggtggc gcggcggtca
    10861 gtcgcgcctc ttcgatggcg agcggccaca gcgttacatc ctggctgctg cgaaactggc
    10921 aggcagtgct ttcgccttcc ggaatggcgg agaaaaaggc tgtgtcgcgc gggacggtaa
    10981 atcctttcgc cagatcgcct tcttccgtat cgggatgcaa ctgcgccacg ctcattgagg
    11041 gcgtgggtgt gacgtaattg gggctgacca cttccagcgg gcgctgggtg aaacggggga
    11101 attcagcgtc aatttttagc tgcgtgcggg cggtcaggaa gctgaaggcc tcgaccattc
    11161 gctctacata cggatcggca atatccgttc cctgcatgcc cagccgggcg gcgactttgg
    11221 gatgacgggc ggcaaattcg gctcccgttt cccgcagata gctcagttcg cggttgtagt
    11281 atcccagtag ccgtggatcc atcggtctct ctctgaatta aaaaaacgta atgcgactct
    11341 gctccatatc cagcgcgcta cgtacccgga atgcagtcgg atacggctgc gtgaggatct
    11401 cgccgcgaat ttcaaactgt aatgtgttat ggccactctg gcggcgctca tcctgaagtg
    11461 ggatgatttg cagggtggac gctttcagcc ggggttcaaa ccgggtgatg gttcgcaaaa
    11521 tggccctgcg gatatcgtcc cacttgtgct catacagaaa gctgcccgcc agtggcggca
    11581 ggccgtagtt aagtacggac gctgccgcgt acggataacg tgcagcatca atatcatctt
    11641 catggctgac ggtgttgagc agaaatgaca aatcccggcg gatgatctcc ttaatctgaa
    11701 ccgggctgac gctgataccg tggtcgcgtt tctggtttgg ggcgtcgtca cacaggcggt
    11761 caaacagcgt gggcagcagg tgattgcctg gacgctggcg ggaagcgctc acgcgtcgct
    11821 tccttcagcg gtgttaaagg tacaggtgga caaatccagc aggctgatat cgccgtgacc
    11881 ggtgagccac accctctgac ccagcgcctg taccagtgtc tctcccggac cgtcctgcca
    11941 gaccgtttcc cggcacagac gcagggagtc ggcagccttt tcagagccgc tgtagcgggg
    12001 aaacagccag gcactgtgtg tatcgccatt gttcagggtg acgttgacgg gtttccagat
    12061 caaatcgatc agatgcgcag gccgcggcga tttcagcgaa cagatttgtg aaaacggcag
    12121 ccagatatat gctccgcccg cgatcagttc gagtaccgga ccggtacggg agtcgctgtc
    12181 gctaatccag tcaaatgcgc cgccattcca ctgcccggaa gtgtctgtta tggcctcaag
    12241 cgcgcggctg cggtgagcat cagcttcctg cgcttcacca cgggcgttac aggcgagggt
    12301 ggtcagcaga tcctccatcc aggcgggcgg cgggagaata acaccgggcc gctgttcgcc
    12361 ctgaaagcag gcgtggcggt atatctcaca gcggatcagc tcgccaaata cctgcgcttc
    12421 gcgagtataa tttgcgtcca tccgggcgca tagctggatc tgttgcagtg cccgcgacca
    12481 gtcgcctgcc acgcacaata actgaaacag gctgtggcgg cgggaggcct gcgctgggtg
    12541 catcctgacc tgttgctctg ccatctgaat gccctccacc acggaataat cccgcagcag
    12601 cgccgacaag gtaatcggta gctcgttatt gtttgtcatg atatatctct ccatgttgtg
    12661 ccgggtttat ccggtaatgg ttgtcaatgc caatgatgcg atgctctttc cgtgtcagat
    12721 ctggcagtac ggtgttatgc gctgtttccg gggcgtattc cggggaaagt aaatgcagga
    12781 tatcaggggg agtcttttcg acaggccaga tagtttcatc ttcgccagtg gcatccagta
    12841 cattgagaat agcgtcaatg cttaacccac cagtgaccat atcctgcaat gtctcggctt
    12901 cggtgaccag gtcacgcaat gaatccagat cctgcgagga gggctcttcg ttaaaaggaa
    12961 aaggatcagc atggcgatta agatcttgtt cgggagataa caacgcctcg cggtattcct
    13021 gataaagtcg ggtcagggca tttccttttt ccgcaccttc tccggtggag gaggctccat
    13081 gaacaggaat gatatcgaat ggattttgtg atgtcagatg ctgctgcctg aaccaatcca
    13141 gatccagata ttcggttatt gcttccgacg tgatggcagt ctgcgatatc gtttttactg
    13201 tgctcaggtc cggagatgat ggattgccat gtacaaggca ctatgaagat aacccccatt
    13261 ctatcaggtc gccttcattc agacgcatac ggtgatgagg ttccacaatc tggtcgttta
    13321 ccgtacagca atattcggta gactgattga taatccacca tgcttcttcg tggcgggtca
    13381 gagtgagggc aatctttaca tcatcattca ccttaaaaac aggaatccag ggagaggtaa
    13441 cggtgaagat aatttgtgtt ccttcagtcg cgtaaattgt gttatcggcg atcccgtgcg
    13501 cctgtatttt ttggagtccc cacgtaatat ttggtttcat tgtg
//
LOCUS       DADLTF010000110        13475 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.110, whole genome shotgun sequence.
ACCESSION   DADLTF010000110 DADLTF010000000
VERSION     DADLTF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.110"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1278)
                     /locus_tag="JN749_002992"
     CDS             complement(<1..1278)
                     /locus_tag="JN749_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAY7369328.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFE"
     gene            complement(1303..2340)
                     /gene="ypdE"
                     /locus_tag="JN749_002993"
     CDS             complement(1303..2340)
                     /gene="ypdE"
                     /locus_tag="JN749_002993"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAY7369329.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(2340..3423)
                     /gene="ypdF"
                     /locus_tag="JN749_002994"
                     /pseudo
     CDS             complement(2340..3423)
                     /gene="ypdF"
                     /locus_tag="JN749_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(3438..4685)
                     /locus_tag="JN749_002995"
     CDS             complement(3438..4685)
                     /locus_tag="JN749_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAY7369330.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(4707..5033)
                     /locus_tag="JN749_002996"
     CDS             complement(4707..5033)
                     /locus_tag="JN749_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HAY7369331.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(5252..6217)
                     /gene="glk"
                     /locus_tag="JN749_002997"
     CDS             complement(5252..6217)
                     /gene="glk"
                     /locus_tag="JN749_002997"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HAY7369332.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            6421..7677
                     /locus_tag="JN749_002998"
     CDS             6421..7677
                     /locus_tag="JN749_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HAY7369333.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            7792..8118
                     /locus_tag="JN749_002999"
     CDS             7792..8118
                     /locus_tag="JN749_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HAY7369334.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(8259..9497)
                     /gene="mntH"
                     /locus_tag="JN749_003000"
     CDS             complement(8259..9497)
                     /gene="mntH"
                     /locus_tag="JN749_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HAY7369335.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            9833..11035
                     /gene="nupC"
                     /locus_tag="JN749_003001"
     CDS             9833..11035
                     /gene="nupC"
                     /locus_tag="JN749_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HAY7369336.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(11085..13274)
                     /locus_tag="JN749_003002"
                     /pseudo
     CDS             complement(11085..13274)
                     /locus_tag="JN749_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
BASE COUNT         3340 a         3669 c         3336 g         3130 t
ORIGIN      
        1 ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca tttcggtacg
       61 gaacagcccg acaccttccg cgccgttggc aaacgcgcct ggcgcttcca gagcggtgcc
      121 gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct gcgcggcagc
      181 ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgc gcgacctgat aataaccgct
      241 caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca ctgcaggttg
      301 cccggcataa cgggcaatcg catccagcgg caagccactc agtaccggga tcgccgaggc
      361 acgggccaga atcagtgtat gcgaggtgcg cccggttttc tccagaatca tacccgcaag
      421 atttttcaaa tcaaggctca aaaactggct tggggttaaa tcttcagcca ccagaatggt
      481 cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga tatgcagcaa
      541 ctgttcgctg atatcgcgaa tgtcgctgac ccgctcgcgc agataatcgc tggcagaagc
      601 agatagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca gtccctgatg
      661 ctgttctgtc atcaggcgac ggatattgcc tgcaaattca tcatcctgaa tcagcgacaa
      721 atgagcgctg aggatagttt tgctttcgcc gtcacgctca cgcaattgct gattcagttg
      781 ctcggcaagc gttgccaggc tgtgctccag ccgggtggaa tcttgcgcac tggcggggat
      841 tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca cgccgacgcc
      901 gcttgccagc acattgccgt acagtaaatc cgggttaagg cggcttaatg aacgcggcag
      961 cggatgcgcc gtcagttctg cctgcgtcgg ctgaacgctg tcgctgtcga taaagcgcac
     1021 caggatgtac tcttccagca cgcgccgcgc ctgctcttca tcgctgccgc taatgttcag
     1081 gctgcaactg tcattaaata aggtgccagt tccaatcaac gccagcgagc ttttagcatc
     1141 ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact ggctgcactg
     1201 ttctttaagt tcccgcgccg gacgagcatg tagaccgtta ggcataggca aaggacagag
     1261 aaattgaatc gttaacataa ccgctccggg ttaatcagga gatcatctga aatccgtcag
     1321 ttgaacaacc gtctcacgcg taagacgttg aataagggca gataaaagtt gctccatctg
     1381 caaaatgtcg cggcaatcgg caatcgatgc cgcgcaatgt ccgtggcggg tggcaggccc
     1441 cattaccaat gtgggtacgc cagtaccggt taagtgcacc gctccaccgt ccgtgccgcc
     1501 gttactgaac atatccgcct gcaacggaac gccaatttct gccgccacgg tttcaatcca
     1561 cgcggtaagt tttggcggcg caatcagcga tttatcgctt aacaccagca tcggcccgtt
     1621 accaatctga cgatggttag ccgcgccata atcaaagttt ttcgcccagc aggcggtatc
     1681 aagcacaatg gcgacgtccg gcgacaccgc gcgggtagca gtttgcccgc cgcgtaatcc
     1741 cacctcttcg ctggaacttg ccaccagcca cacttccgca ggcagttcgg cgtcgtgcag
     1801 ctcacgtagt aacgtcacca gcagatagca accgaggcga tcatcaaagg ctttgcccat
     1861 cacccgctgg tgagggagga cctgaaaagt ggtatcgaag gtgacgcgat cgcctggacg
     1921 aattcccgcc tgcatcactt cgtcataggt gcgcgcgcca atgtccacgc gcatggcgct
     1981 aacgtcattc ccctgccggt cgccgtcaag caggcctaga attttgcact cttcacgggt
     2041 ggtgatgcgc accggctgca gctggcgggc agccatgcgc acgttgccaa ccggcagcac
     2101 gtcaatagcg ccttcgcggg agatgctgcg caccataaat cccacttcgt ccatatgggc
     2161 gcagatcatc accttcggac ctgtcgattc attgaggcgg atcagcaccg atcccagacc
     2221 atcaaatcgc acttcttttt gcaggcgagc cgcttcttcc agcaggatct gccgcacttc
     2281 ctgttccgag gaggcgatcg catctgcctc gctcaacgct tttaatagcg ataaatccat
     2341 tatgcctctc ccgtgagcaa cactgttttc ggcatggcgt agagcacttc tgcgccttgc
     2401 ggggtaacca gcacaacatc ttcgatgcgc acgccccctt gccctagcaa ataaatcccc
     2461 ggctccacgg tcagcaacat gcctggctgt agcgtcgtgg tgtcccgcgg tgaaaaacgc
     2521 ggatcttcat ggacttcaat gccgatagcg tgaccagtgt tatgaccgaa atagtcgcca
     2581 aatccagact cggtaatgac ccggcgcgcg gcgtcgtcaa tctgctggca gcgcacgccg
     2641 gggcgaattg cagagattgc cgcgagctgt gcctgcagga caatctgata gacgtcaaac
     2701 agcgggtgag attcggcgct caccccttcg ccattcacca gcaaggtgcg cgtcatatca
     2761 gcagtagccc tgatacagcg caccaaaatc gagagtgaca aactcgcccg ctgcaacaat
     2821 cttgtcgctg gctttgccgt gcggcagcgc cccgcgccag ccgctggcga caatggtgtc
     2881 aaaagaggct ttttctgcgc cctgctggcg cataaaccac tccagttcag cggctatctc
     2941 gcgctcgctc atccccgcct gaataaagcg gcgaatatgc tccgctccgc aatcggcaat
     3001 cccacaggcg aggcggatta tctccacctc ctctggcgtt ttgatttgcc gcagcacatc
     3061 cggcgtggcg ctaaccagtt tcgcattgag ttcagacttc cagcggtgcg cggtttccca
     3121 actcacctgc tggccttcaa aaccgagcgt ttgcaactgc tcatcggcaa tgatttgatt
     3181 gacgatagtg gtaagcgtgt tcgtcgcgtc aagcaaatgc agctggtagc cttgcgcacg
     3241 ggcttccacc tcaacgaaat agcgcgaatc taccagaatg tgcgcacttt cacggctaat
     3301 aaccacatag cctgatccgg tggagatccc cagatgcggc tgtttgttct gccgtgagga
     3361 gagaagcacc gcatccagtt gttgcacctt aagccagttg cgcagcgaag cgagtaatgt
     3421 catctggatt ttccttctta caggctatcg attaacaatt tgcctttgcg gaacatcatc
     3481 agacgcagga acaccaccat cagcgcagta atgattgccc ccagcgcgat ccccgccata
     3541 taaacgccca ggttagtgac cagcggccat gcccagatag cggattccgg gaaccattgc
     3601 actgcgccca gccagacagc tgcggttgag ccgacaatcg cgccgaccat atatgacgga
     3661 atggcggtga tggggctttc cagcgcaaac ggaatcgcac cctcactaat ccccataaag
     3721 gccaggaaca tcgcggtctt accctgcgga taaagttgag cgttgaacag gcgtttgccg
     3781 gttaaacgac ggtcaataat ggtcgccaga cccagaccaa tcggcggaat aacgatagcg
     3841 atagaacgag cggtgaccgg taatacgtgg tcagtggtaa agctgaaggc aacgaaacct
     3901 gcggctttgt tgatcgggcc accaaggtcg attgctgtcg ccgcggcgat ccccattgcg
     3961 tacatcagcg cgcccttctc acctgcggca gtcagcacag tacggatacc gccgttgatc
     4021 cagccaccaa agggggtgat gacgtagtac atcgccagca tgacaaaaat ggcggaaaga
     4081 atcggtagca aaaatgtggt tttgaacgct aacaggaaat ccggtaactg gattttctgg
     4141 ttcatccatt tcacaaggta accggcaacg atagaaataa tcagcgcacc aatgaaggtg
     4201 gacggcaccg gcgaagaggt cgcccattgc atagtgctgg gatcgaagtt cagcagttgc
     4261 gtcggctggg tagacatcaa cccgccgata aaaccagccg ggaacgccag tttgccaccg
     4321 atagagttcg ccacaaatgc agcaaacatc ggaatggcaa aaccaaacaa cacgccgccg
     4381 aaggactgcg acagccaggc aaatttaagt aaggagaggt cgaaaccgga gaatttcccg
     4441 ctattaagag catccatgat gccgatctca gcaggaattt taagccagct ataagcaatc
     4501 agctggctga aagcgaggat cacaccgccc ataattaagg tcggcaccat gcgtgaaatc
     4561 cctgacatca catgctgcgg gagttcaccc cagaagctgc ttttagaggc ctgcggattc
     4621 ttaactgccg ctgccgcacc ggatgcgcca ggcacaacgg ttgcactgcg ttttttaatg
     4681 gccataacaa ttatccctgt cgaatattac tgttgttcag aggcaatcat ctcttcgatt
     4741 tctttgatga tgcccgcagc gtttttaatt gcgtcctgca aagtgatttc ataaacgtcg
     4801 cgtgattcga aacgttcgtt atcttccggg gtaactgcca cggaatggat gatgatggtc
     4861 gcttcggcga tatcctgcgc cgtcaggcga ttctggatgc cgtccgcgcc ctgggtttca
     4921 attttcactt cataaccggc ttctaccgcc gcttcttcca gcgcctgagc ggccataaaa
     4981 gtgtgagcca ggcccattgg gcaggcacat aaggcaatca gtttcttact catcgttaat
     5041 tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt gcaaaacaaa tcgccgttac
     5101 cagacaaata atgcatttac tggagaatta atcccgctgt acggaagtgt gacgcaggtc
     5161 ggccttgtga ggggacacaa agaaaaattg aattaactgt atgatttaaa atattatcgg
     5221 gagagttacc tcccgatata acaggaagga tttacagaat gtgacctaag gtctggcgta
     5281 aatgtgcacc ggaaccgaga agacccggat agtcatggac gatgagatac accggaatat
     5341 catggacata ttctttaaag cgccctttat cttcaaatgc ggcacggaaa ccggaggctt
     5401 tgaagaactc aaggaagcgc ggcacgatac cgcccgcaat aaacacgccg ccaaatgtcc
     5461 cgagattgag cgccagattg ccgccaaaac ggcccataat gacgcaaaac agcgacaatg
     5521 cgcggcggca atcggtgcag ctgtcagcca gcgcgcgttc ggtaatatct tttggcttga
     5581 gattttctgg caggcggttg tcagctttca caattgcgcg atacaaattc accagcccag
     5641 ggccagaaag cacgcgctcc gccgaaacat gaccaatttc cgcacgcaat atttcgagga
     5701 taatggcctc ttcttcacta ttcggcgcaa aatcaacgtg accgccttcg cccggcaagc
     5761 ttacccaacg cttatcgaca tggaccagat gcgcaacccc aagccccgtt ccggcaccgt
     5821 aaaccgcaat aggcttacct tcgaccggtt ctgcgccacc aaactgaatc agatgctctt
     5881 ttttcagcat cgggatcgcc atcgatacag cggtaaaatc gttaataatt tccagatggc
     5941 taaaaccgag attctttttc atttcggcaa ttgagaacgc ccaggtatgg ttggtcattg
     6001 ccacccagtc accggtaatt gggcaagcga tggcaataca gccgtctttc acctcgacct
     6061 tatgttcttc aagataaacg cgaatgaccg cttcgaggct ggggtaatca agccctgaat
     6121 aggtcttagc ctgcgagatt tcaccactgg caatatcaca cagagcaaga cgtgcgttgg
     6181 tgccgcccac atcaccgact aatgcatact ttgtcattct tcaactgctc cgctaaagtc
     6241 aaaataattc tttctcacac tgtaaatact tgggggcata acaacaacgc cggaaaggca
     6301 ggctccctgt aaatatcgat ctgggtcaca caattacttt atcgtttcag caccaattgc
     6361 agcgatgcct ttttgcaagc tgggcaaact aagtatctga ccccgcataa ggaatagaac
     6421 atgctccatc cgcgagccag aaccatgttg ttattatcgc tccccgccgt ggcaattggg
     6481 attgcgtcca gtcttattct gattgtggtg atgaaaatcg cctcggtatt acagaatttg
     6541 ctctggcaac gactgccggg aactctgggg atagcccagg attcccccct ctggatcatc
     6601 ggtgtattaa cgctaacggg tattgcggtg gggttggtta tccgtttcag ccagggtcat
     6661 gccggaccag accccgcctg tgaaccgctg atcggcgcac cggttccgcc ctctgcgcta
     6721 cctggactta tcgtagcatt aattctcggt cttgctggcg gcgtcagcct ggggccggaa
     6781 catccgatca tgaccgtcaa tatcgccctt gccgtcgcca ttggcgctcg tctgttaccg
     6841 cgcgtcaacc gaatggagtg gactatttta gcctctgccg gaaccatcgg cgcgctgttt
     6901 ggtacacctg ttgcggcggc gttgatattt tcgcaaacct taaatggcag tagtgaagtt
     6961 ccgctatggg atcgtctttt tgcgccgtta atggcggcag cagctggtgc acttactacc
     7021 ggattatttt tccatcccca tttttcactg cccattgcgc attacggaca gatggaaatg
     7081 accgatattc tcagcggtgc aattgtcgcg gcgattgcca tcgcagcagg gatggtcgcc
     7141 gtatggtgct taccacggtt gcacgcgata atgaatcaaa tgaaaaatcc ggtgctcgtg
     7201 ctgggtattg gcggatttat tctcggaatt ctgggggtta ttggtggacc agtttcgctg
     7261 tttaaagggc tggatgagat gcagcagatg gtggcaaatc aggctttcag caccagcgat
     7321 tactttttgc tggcggtaat taaacttgcc gccctggtcg ttgctgccgc cagtggcttt
     7381 cgcggtgggc gaatcttccc ggcagtgttt gtcggcgtgg cattagggtt gatgctgcat
     7441 gagcacgttc ccgccgttcc agcggcaata accgtttcct gcgctattct cggcatcgtg
     7501 ctggtggtaa cacgcgatgg ctggttaagt ctttttatgg cggcagtcgt tgtacccaat
     7561 accacattgc taccgctgct ctgtatcgtc atgcttccgg catggctgtt attagcaggt
     7621 aagccgatga tgatggtcaa tcgtccgaag caacagccac cccacgataa tgtttagcaa
     7681 aaaatgctct cctttatgat ttaagagtta tggcgatgat acggggcttt acctccccgt
     7741 aatattgcgt taacaggccg ctgacggctt cgatcgtgaa ggagaataac aatgttcagg
     7801 tcactgtttc tggcggccgc cctgatggca tttaccccgc ttgcagcaaa tgcaggtgaa
     7861 atcaccctac tgccatcgat caaattacaa attggcgatc gcgatcatta cggtaattac
     7921 tgggacggtg gtcactggcg agatcgtgat tactggcatc gcaattatga atggcgcaaa
     7981 aaccgctggt ggcgtcatga taatggctat catcgtggct gggataagcg taaagcatat
     8041 gagcgtggct atcgtgaagg ctggcgcgat cgtgacgatc atcgcggaaa aggccgcgga
     8101 catgggcacc gccattaaac gcgtcctcca atggagcaca atgcctgatg cgacgctagc
     8161 gcgtcttatc aggcctacaa accggctctt taacgcgtag ggcggataag gcgtttacgc
     8221 cgcatccgcc aatagtgcca gatacgacgc tcattcaact acaatcccag cgccgtcccc
     8281 accaacaacc agatattcag cgccacgacc agcactacaa tcacccagcc tgtctgtttt
     8341 acgcgtttgc tgttcaccag atcgcccatc aacttgctgt cactggtgaa aatcagcagt
     8401 ggaaccagcg ccagggcgat accaaaactt aacagcacct gactcataac cagaatccgt
     8461 gtcggatcta atcccatcag aatgacaata aatgacggca acatggtgac tgtacgacgc
     8521 acccacagcg ggatatggaa gcgaatgaat ccctgcatca ccacctgccc cgccagtgtc
     8581 cccaccaccg ttgaggacag tccggcagca accagactta acccaaagac cgttgccgca
     8641 gcatggctta acagcggttg cagcgtcaga taagcctcat caagatcggc aacaccagta
     8701 tgaccagaaa agtggaacgc cgccgcagct gtagccatca tcgccagatt gacaaaaccg
     8761 gcaatcgtca tggcgatagc cacatcccat ttggtggcgg aataacgttg ttgacgcgaa
     8821 ccgccatgta aatgctgagt gagcgaagag tgcaaataaa tcacatgcgg cataatcgtc
     8881 gcccctaaca cgcctgctgc caggaagacc gcctccgaag taggtaaact cgggatcacc
     8941 attcctttac ccagctgcgc caggttaggc tgggagaaaa tcaactcgac aatgtaagcc
     9001 gcggcaacaa acaacagtaa cccgccaatc actttctcca gcggtttttg cccgcgacgt
     9061 tgcagcatta aaatcaggaa agtcgcgatc cccgtcagca ccgcgccctg caacaacgaa
     9121 acaccaagaa tgagtttaaa accgatcgcc gcaccaataa attccgccag gtcggttgcc
     9181 atcgcaataa tttctgcctg aacccaatag aaccacacta cgggacgcgg atagtgatca
     9241 cgaatctgct ccgccagatt tttaccggtg gcaatcccta gtttggcaga gaggatctga
     9301 attagcatcg ccatcaggtt ggcccaaacg acaacccaca gtagctgata gccgaagcta
     9361 gcacccgcct gaatattggt cgcaaagtta ccgggatcga tataaccaat cgccgcaatg
     9421 aacgcaggtc ccattaatgc gagcctcatc ttgcgcgccg cccgtccgct gctactctca
     9481 acgcgatagt tcgtcatctt gtgcctctaa aacatagcct ttgctatgtt tcatgctatt
     9541 ctaaacgaga atgattatca aattcattta aatggattgt ggtgatttct ctgatagacc
     9601 aagattacga ttacgaaaag attgatgact tgaatgttat ggttatgttt aatgttagca
     9661 catttacata actttcagct tccatacaca acatagcaga aatgtatgac agatcactat
     9721 ttttgaagcc tgtcacagga cgtcattata gtgtgtgtca gatctcgttt tccttaacca
     9781 tgttacatag aatgtgcacg gaaatttaac ctgcctcata tttggagcaa atatggaccg
     9841 cgtccttcat tttgtactgg cacttgccgt tgttgcgatt ctcgcactgc tggtaagcag
     9901 cgaccgcaaa aaaattcgta tccgttatgt tattcaactg cttgttatcg aagtgttact
     9961 ggcgtggttc ttcctgaact ccgacgttgg tttaggcttc gtgaaaggct tctccgaaat
    10021 gttcgaaaaa ctactcggat ttgccaacga agggactaac ttcgtctttg gtagcatgaa
    10081 tgatcaaggc ctggcattct tcttcctgaa agtgctgtgc ccaatcgtct ttatctctgc
    10141 actgatcggt attctccagc acattcgcgt gttgccggtg attatccgcg caattggttt
    10201 cctgctctcc aaagtcaacg gtatgggcaa actggaatcc tttaacgccg tcagctccct
    10261 gattctgggt cagtcagaaa actttattgc ctataaagat atcctcggca aaatctcccg
    10321 taatcgtatg tacaccatgg ctgccacggc aatgtccacc gtgtcgatgt ccatcgttgg
    10381 tgcatacatg accatgctgg aaccgaaata cgtcgttgct gcgctggtac tgaacatgtt
    10441 cagcaccttt atcgtgctgt cgctgatcaa tccttaccgt gttgatgcca gtgaagaaaa
    10501 catccagatg tccaacctgc acgaaggtca gagcttcttc gaaatgctgg gtgaatacat
    10561 tctggcaggt ttcaaagttg ccattatcgt tgccgcgatg ctgatcggct ttatcgccct
    10621 gatcgccgcg ctgaacgcac tgtttgccac cgttactggc tggtttggct acagcatctc
    10681 cttccagggc atcctgggct acatcttcta tccgattgca tgggtgatgg gtgttccttc
    10741 cagtgaagca ctgcaagtgg gcagtatcat ggcgaccaaa ctggtttcca acgagttcgt
    10801 tgcgatgatg gatctgcaga aaattgcttc cacgctctct ccgcgtgctg aaggcatcat
    10861 ctctgtgttc ctggtttcct tcgctaactt ctcttcaatc gggattatcg caggtgcagt
    10921 taaaggcctg aatgaagagc aaggtaacgt ggtttctcgc ttcggtctga aactggttta
    10981 cggctctacc ctggtgagtg tgctgtctgc gtcaatcgca gcactggtgc tgtaaaacca
    11041 tacataaaaa agccggggat cattccccgg cttttttacg cctgttaatc agctaatggc
    11101 tgcgggcgac caatcaaata cccttgcaga tattgcaccc cgagcttatg caatagcgcc
    11161 tgctgctgtt gcgtctcgac aaactccgcg accacactca atgacttcgc tttcgccaga
    11221 tcggtaattg atctcacaat catcgcatcc agcgtattcg tgacaatatc tttcacaaag
    11281 acgccatcaa ttttgatgat atcagcctgc aaacgcttta accgttcgta gttggcatat
    11341 ccggtgccaa agtcatcaat cgcaatccgg aaaccaaact tatgcagctg ctcgatgttg
    11401 tacatgctgc tttctgcgtt agaaaacgcc tgctcctcgg tgatctcaag aatgaccgcc
    11461 tgcggggaga tgtgataacg cttaaacaga cgaataatcc gtccggcaat attcttttgc
    11521 agcagcgtga gcggcattaa attgactgaa aagcgcggtc cttttttgtc gcaagggtgt
    11581 gttgccagcc acttcaacaa ggattccagc acttgcaaat caaaacgcgc gctaaggtta
    11641 aactgagcaa taaggggcag aaacttatcc ggggtcataa tgccgccgtc atatttcagt
    11701 cgcgcgagga tctcatcata accttcaccc tctttgttgc gaattggctg ggcgtagagc
    11761 agcaaatcac cctgatctaa cgccgtgcga atggtattca gcaatagcac ctgtttagtg
    11821 gtctgcccgg aaaccatctc ctctctgcta tccagcgcca gcacatgatg atgtgcgcag
    11881 gattgctccg ccagccagct taactgcccc aacaagggtt gcagggtttc ctgatttcca
    11941 tcaaaacgcc cccaggcagc accatagccc atatccagcc cggtattgtt ccagtgaatt
    12001 tgccgactat tcaggatgtt aaccatatgc tggagtcgcc cttccgtttc cggcccgctc
    12061 agcactaaca gcagttcact acccggcaat tgatacaact tttcgttttc ctgcatcaac
    12121 ggcagcagcg tacggcaaat tgagcggata cagtgaacgc gcatcattaa gccgtaatga
    12181 cgactcataa actcaagatt atcaatgcgc aggcagcaaa aactcttgcc cgcctcttgc
    12241 tccggcgctt gttccaacgc acgaaagttg ggtaggagcg ttaacggatc tgttagcgcc
    12301 tgcaaatgcc agcggcgatt aagccattca ctgcgatgat aaatgcgcac catatagagc
    12361 aggcaaacgc taaaggaaat cagcaccgcg agaataaatg ccagcgaata ttcggtttcc
    12421 accccttgca aaaagttctg gttgtaattc agaaggcaaa gcgttgaaac agcctaggtg
    12481 agatttaaaa acggatagcg aagattaccg accccgaggg taaagatgat gaagaaaacg
    12541 ggtaccaggt aaccggcaat aaagtcgttt tcataaggtg tgcacaacag aagtagcagc
    12601 acgcttaacg ctgccagcca gcttaaggta aatgcgcgtt tctctttgcc caacgacgga
    12661 gcgatatccc tgcgccacaa tatctgcgca aagtggggac ttacaatcat gcgagtgaga
    12721 tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa ggcttagtaa atcaacgacc
    12781 gtgaaaatgg catccgcatc gccgaaaaag gtggatatct ttagcgggaa atcaaagaag
    12841 ctccccacaa gatacatgct gcatttgatg ccgatcggcg tcaccaaacc aagccagaac
    12901 agacgttgcc agacatatcg gctggtcaat ccataacgcc aacgcgtccc cagctgccag
    12961 cgtaatattg cgcaggcaca caacacgaca aacgtctggc tgccgagcat gaccgcagtc
    13021 tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa acatcccgca taaaacggga
    13081 acgacaccgc gccagccaaa gataaacaag attgacagca ttacgcacag tggcatccac
    13141 gccagaaaga tataactgga atggataatg gcaagtggcg aaataaaacg ggatagctga
    13201 ataagtacca cggtgagcgt aaacgctagt gtgaatatct tgatattttt tatcaggtta
    13261 tgctccacaa acatgagtac actactctca ctgatcgggg ttttcaggca cacaaataat
    13321 aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt tgccgatttt tcatccaatg
    13381 atgctttcac caggcggcgc taacccggac gtaaactgta ttacaaaagc ggcaaaaagc
    13441 agagacaaaa aacccccgct ttgcagcgag ggttg
//
LOCUS       DADLTF010000111        13438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.111, whole genome shotgun sequence.
ACCESSION   DADLTF010000111 DADLTF010000000
VERSION     DADLTF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.111"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..352
                     /locus_tag="JN749_003003"
     CDS             <1..352
                     /locus_tag="JN749_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7369337.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            509..1258
                     /locus_tag="JN749_003004"
     CDS             509..1258
                     /locus_tag="JN749_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369338.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1280..1846
                     /gene="yieF"
                     /locus_tag="JN749_003005"
     CDS             1280..1846
                     /gene="yieF"
                     /locus_tag="JN749_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HAY7369339.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1900..3237)
                     /locus_tag="JN749_003006"
     CDS             complement(1900..3237)
                     /locus_tag="JN749_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAY7369340.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3402..4067
                     /gene="yieH"
                     /locus_tag="JN749_003007"
     CDS             3402..4067
                     /gene="yieH"
                     /locus_tag="JN749_003007"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HAY7369341.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4134..4607
                     /locus_tag="JN749_003008"
     CDS             4134..4607
                     /locus_tag="JN749_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HAY7369342.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4656..5243
                     /locus_tag="JN749_003009"
     CDS             4656..5243
                     /locus_tag="JN749_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369343.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5305..6027)
                     /locus_tag="JN749_003010"
     CDS             complement(5305..6027)
                     /locus_tag="JN749_003010"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAY7369344.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPILGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNMLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6029..7204)
                     /locus_tag="JN749_003011"
     CDS             complement(6029..7204)
                     /locus_tag="JN749_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAY7369345.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYLLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7231..8847)
                     /locus_tag="JN749_003012"
     CDS             complement(7231..8847)
                     /locus_tag="JN749_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HAY7369346.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8933..10330)
                     /locus_tag="JN749_003013"
     CDS             complement(8933..10330)
                     /locus_tag="JN749_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAY7369347.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="JN749_003014"
     CDS             complement(10346..12223)
                     /gene="bglF"
                     /locus_tag="JN749_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HAY7369348.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="JN749_003015"
     CDS             complement(12357..13193)
                     /gene="bglG"
                     /locus_tag="JN749_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HAY7369349.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         3547 a         3373 c         3080 g         3438 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg gcgctggaca atgtgttaca
       61 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
      121 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
      181 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
      241 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
      301 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
      361 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
      421 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
      481 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
      541 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
      601 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
      661 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
      721 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
      781 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
      841 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
      901 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt
      961 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     1021 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     1081 cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     1141 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     1201 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     1261 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     1321 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     1381 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     1441 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     1501 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     1561 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     1621 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     1681 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     1741 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     1801 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     1861 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
     1921 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     1981 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
     2041 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     2101 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
     2161 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
     2221 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     2281 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     2341 actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat ccgccagacc
     2401 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa
     2461 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     2521 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     2581 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     2641 ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag
     2701 attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc
     2761 catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat
     2821 gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc
     2881 ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa
     2941 agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa
     3001 tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt
     3061 gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag
     3121 gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt
     3181 aaaaacgcgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat
     3241 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc
     3301 gcgtttgtga gagacttttt tattggattt acttatacgg caacgattgc gttgcgcaaa
     3361 tcggctctac gaaaacgtta aactgattaa aaaggaaagg catgtcccgg atagaagcgg
     3421 tatttttcga ctgcgacggt acgctggtcg acagtgaagt catttgctcc cgcgcatatg
     3481 taacgatgtt tcaggaattt ggtattacgc tcgatcctga agaggtattc aaacgtttca
     3541 aaggtgtaaa actgtacgaa attatcgata ttgtttccct tgaacatggt gttacgttag
     3601 cgaaaacaga agctgaacac gtttaccgtg cagaagtcgc tcggctgttc gattcagaac
     3661 tggaagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca atgtgtgtgg
     3721 tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat atgttgcact
     3781 acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca gacccggcgt
     3841 taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt ctggttgatg
     3901 actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc tacttctgcg
     3961 ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc catctttcgc
     4021 agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc ttcacactcc
     4081 cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt gatatgtctg
     4141 tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg ttaattggtg
     4201 ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt ttggtaataa
     4261 tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt cctgcattgt
     4321 taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa aattatcgtt
     4381 gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca gttattgtac
     4441 atattaaggg catggcttcc tcggagttgt ttgaaaacat tctttctggt gacagtctcg
     4501 ttgtccgcat cattcctgca ttgctggcag gtgtggtgat gagcagaatc attacccgtc
     4561 tgcccggatt ggatatttca tgtcctgaaa cagactcttt aagttaagcg ggatacttta
     4621 tctttgggct actcaaaagc agacaggatg ttactatgac tcaaaatatc aggccgttac
     4681 cccaattcaa atatcatccc aagccactgg aaacaggcgc atttgaacag gataaaaccg
     4741 tagagtgcga ttgctgtgaa caacagacgt cagtttatta ctcgggtccc ttttattgcg
     4801 ttgatgaagt tgaacatctc tgtccgttgt gtattgcgga cggttctgct gctgaaaagt
     4861 ttgcaggtag ttttcaggat gatgccagca tagaaggtgt tgaatttgag tatgatgaag
     4921 aggacgaatt tgccggtatt aagaacacat atcctgatga aatgctgaaa gagttggttg
     4981 aacgcacgcc aggttatcat ggatggcaac aggaattctg gcttgcgcat tgtggcgatt
     5041 tctgtgcttt tatcggctat gtgggctgga atgatataaa agatcgcctc gatgaatttg
     5101 ccaaccttga agaagattgt gagaatttcg gtattagaaa ttctgatcta gctaaatgcc
     5161 tgcaaaaggg tggtgattgt cagggttatc tcttccgctg tctccactgc ggcaagctga
     5221 gactgtgggg tgatttttcg tagttattta aataatgaga acaggccgga gcgtaattca
     5281 tacatccggc cttatttctt cagcttaacc gaacgctaat tctgcggcag cggctttatc
     5341 agcgattacc atcagcgaag gatgcagctg caacacggaa gcaggaacat cttcggtaac
     5401 cggaccttgc agcatatttt tcagtgcctg cgctttacct gcgccactga caattatcag
     5461 caagtttttc gccgccatga tgcttttcgg ccccatcgtc acgtagctgt caggcactaa
     5521 cgaaaagtcg ccgcccaact caccatgcgc cacaatatca accatctctc ccaggatcgg
     5581 gaactccacg gtttgctcat ggaaatgggt cgtattcggc aaattgccac aaaaatgacc
     5641 atctgcacct aatcccagca ccaccaaatc caggccgcct tcacgcgcca gtttctgatc
     5701 atgctcgcgg tagttatcaa tagtgagctt ctggatattc tcttctttga tccccgcagg
     5761 ggtgaaaaac agattacgca gattggtaat cgttacgcct tctccctctt tgccgcgaaa
     5821 gggaatttca tcgaaattat agaaatagca gttatcgtac caaggcttac ctttaaccag
     5881 ggtggtgagg tattcataca tgcctttggg cgtgctaccg gcggtaattg ccaggttaac
     5941 acgacgcgtc ttcgacatat aaccaagcag atggtgtgcc gcgacacggc tcatttcctg
     6001 gtaatcttcg gtaatgatta atttcatctt aatgccttat tttaaatttc tgtacgaagg
     6061 ctgcataggc aggccgccag acatccattt catggttcag gccgggatat tcctggtaat
     6121 caaacttaat ttttttctgc tcaagctcag ttttcagccc ggcgatatcc ttgccggtta
     6181 cgacatcttt atcaccaacg accacagtaa aattacgtag ttgctggtta atagctgccg
     6241 gatcgttcag ccgggccgcg acaccttcat ccggtacggt tgtcgtggta acaccactga
     6301 atgtggccag ccagccaaag ctttccagat gattcattcc ggaaaccagc gcctggtacc
     6361 cgccttgtga aagccctgcc agcgcgcgac catcggcatc tttacggaca ctaaaacgct
     6421 tgctaatcag cgggataata tcgttcatca gttcgcgatc tgccgcttta gcattcagca
     6481 gataaaagac tttacgtctt tcctgaggca cgaaatcttc gggaataatg cccttcgcat
     6541 cggtttcggt atccgggatc accaccagca tcggtttaat tttcccttca gccagcaggt
     6601 tatccatgat ttgcgggata cgcccctgat cgatagcgga acgtccagta tcaccaaaac
     6661 cgtgatagaa atagagcact ggcaaaggct cgcccatgcc ggtgtatcct ggcggggtcc
     6721 agacatacat ctgacgttca gattgcaatg cgttggagtg gtaagttatg gcgatcaaat
     6781 caccatgcgc aacagaacgc gtatccagat aactgcctag caccagaatc atactggagt
     6841 taacctggcg ctgaggctta gtcattgcgg tgcctgtatc aatgctgcgt acaccatcaa
     6901 cattgaaaaa atactcgtac aggttgcctt tcaggatagg tgtgcgccac gaccagactc
     6961 ctgcttcgtc tttggtcatc gggtgaatat tgtccggaac cggaacaccc accactacag
     7021 agacattttt tgccccaggg gcaaagtagc gaaaggtcac gctgttatcg gcattgacct
     7081 gagtcacata ctgtttcacc gggatagtgg gtgccgggct ggctggcata tccgctgcta
     7141 tggcccactg agcagaaata ccgctggcaa tagccagcgt taaagcagca atttttatat
     7201 tcatcgtaat attcctttca ttaagttcca ttaccaccag atttcagcct gggcaccgac
     7261 agcaaactgg tcgtctttat tgtcttcgaa ggtgaagcca tccagttcgt tttccagggc
     7321 tttgatatac gtggcgtaga aacgaatttc cagacgcgag gtcaacatac tggtattgac
     7381 tttaaaggta tggaagagcg tggttttata accagactca ttaaatttat tactattcgc
     7441 atctttgttt tgctgggtga aatagcccag ttcaacacct gtctggttat attggtccca
     7501 aatataggcc gggcgaacaa ccgcacgaat agattcgaaa tcagagtgtg cgcctgtttc
     7561 gtagctataa atatcgttac cgaaggagta aacaatcgcg ttagccacaa taaaatgatc
     7621 gccgatatag gcttcgccct gcgaggtcag acgaactgct gttccgccgg tgtgatcacc
     7681 ataataacga ccattaaagg tggtaaatgg acttgcgcca gcataacggc caaagttcct
     7741 ggcgatagag ttattcgcaa ccaggaagga gaattcgttg aagccacctt tatcaaattt
     7801 ctgcgttaaa gatgtgccga acatccaggt atctttccag tcataatacc cgttattatc
     7861 ctgattatct ttttccgatg cgctttcgtt tgccgtgaca taacgaccac tcaccattaa
     7921 ggtcgcttta tcccataacg ggatatcttt atagcgtaaa tcaatagtat gggtattaat
     7981 ctgctgtttg ttttgcaggc tgcgatcgta atcatcgaca tcttcgcgaa ccagcgcgat
     8041 atcaatttta cccggaccga ctttccagtt ttccagacct acacccgctg cggcatcagt
     8101 acgctgcgtt ttccagtcca gcatctggat ttcaattttc ggcgcaccgt gtttacccac
     8161 ccagaaatca gcctctggcg caaagggcag gaaacctttg gtggtaacgt acatatcgga
     8221 gaactgcata aagttctcgc caccggcgtt atcgccaaac cagccggtag agtactgctg
     8281 accaacgtta ccatccatca tcacaacggc atcaacccgt ttgccgtttt cgttgtagac
     8341 acgttgtttt aactgcaaat caaaccagcc ggagtattcg ttaccaaagc ggcccagaga
     8401 accaatcgcc catgatttag gtgaaccatg agaggcggtc ccccaaccag aacggtaata
     8461 gccgttgtaa ctaaagccaa tttcatcttt cacaaactta ctgaaatctt tcagagtcat
     8521 ggaagaataa atcgagcctg tctcactggc atttttctct tcatttttga ccagtacagc
     8581 atcaggtttt gcggcactac tggtcgggaa cggattggcg gcataccctt gatcattcgt
     8641 acttacgcta cgattcaccg tcgctggcgt atattttttc ttctcttcat ctttatactt
     8701 tttgagttcg ctttgcgttt ctcttaacgc tttttctaat aactcaaggc gttgttccac
     8761 cgttaatgat tcagccaatg attgtgcaga aaaggctaac ggtgccatta ataagatggc
     8821 agaggtaata agatttcgtc taaacataat atccctttat ggtgcaaaga aagaattaac
     8881 gtatcgcatc cagactgttc tgaatgcgac gataattaag gtgctttatt ggttattttt
     8941 ttaatgacag cccccgcgtt ttgatcactt ctgcatacca tccgaagctt ttcttacgcg
     9001 ttcttgtgag gcttccttcg ccattatcat cacgatccac ataaataaag ccgtagcgct
     9061 tagacatttg tgaatgagat gcactgacta aatcaattgg cccccaactg gtgtacccca
     9121 taatatccac accatcggca atcgcttcat ttacctgtac caggtgatcg tttaaatagg
     9181 caattcgata atcgtcctgt atcgaaccat ccgcttcaac gctgtctttt acgcctaatc
     9241 cattctcgac aataaataac ggtttttgat aacgatccca aagcgtattt aacagaaccc
     9301 gtaatccaac cggatcaatt tgccaccccc tctctgaact tttcagatgc ggattgggga
     9361 tcatattcag tatgttgccc tgcgcatttt tattaatgct ttcgtcgtgg gaaacacaac
     9421 cagtcatgta ataactaaaa gagatgaaat ctacagtatg ttttaaatct tctgcgtcac
     9481 tttcagtcat ctcaatggtg atattgtggt cgcggaagaa acgctgcata tagccgggat
     9541 actggccacg cgcctgaaca tcaccaaaga acatccagcg ccggttctct tccatggcct
     9601 gcaacatatc ctgtggctgg caggtgaggg ggtaaaccag cccaccgaga agcatattgc
     9661 cgattttcgc ttcggggatc aggctatgat aggctttaac tgcccgcgca ctggcaacca
     9721 gttgatgatg gatcgcctga taaacttccg cctcgccact ctcttctgcc agccccacgc
     9781 ccgtgaatgg cgcgtgtaac gacatattga tttcattaaa cgtcagccat aacgctactt
     9841 tatgttggta gcgggtaaag accgtgcggg cgtaatgctc gaagtgatcg atgaccgccc
     9901 gattagccca accgccgtag tttttcacca gcccatatag catttcgtaa tgggataacg
     9961 ttaccagcgg cttgatcccc gcctgcgcca tttcatcaaa cagccgatcg taaaacgcta
    10021 accccgcttc attcggttcg gcttcgtcgc cctgagggaa aattcgcgcc caggcaatgg
    10081 aaatacgcag acaggtgaag cccatctcgg caaataacgc gatatcttcc gggtaacggt
    10141 gataaaaatc gatggcgaca tctttgatat tctctttccc caggatgcgc ggttccattt
    10201 ttcccattac gccatgaggc tgtaaatctg aggtcgtgat ccctttgcca tcttccagcc
    10261 aggcaccttc cacctgattg gcagctgttg cgccacccca aagaaatgtt tctggaaatg
    10321 ctttcgtaat taactccttt tatcgttagc gaatgatgga taacagcggt tcacctgcgc
    10381 ttatctgcgc cgtgccgtgg ggtaatacgt ctgtaaaatc atcgctatta ctgattaata
    10441 ccggcgtcgt cagatcaaat ccggcctcgc gaatagcagg gatatcaaaa gaaatcagcc
    10501 gatcgcctgt attgaccttg tcacccacgt tgacgtgagc ggaaaagaat ttgccgtcca
    10561 gttttacggt gtcgataccg acatgaatca ggatctccac accatcatct gactcaatgc
    10621 caatggcgtg taatgtggcg aacaacgaag caattcgacc cgcaaccgga gaacgcactt
    10681 caccaaccga gggcagaatg gcgatacctt tacccagcag gccactggca aacgtggtat
    10741 cagcgacgtg aatgagcggc acaatctctc ccgtcatcgg tgaacagata ccgccctgct
    10801 caggtggtgt aataacctct ggtgttttct cttgcggggc accctgcgct ggctgacgtt
    10861 tactggtgat gaaatgaagc atcaacgtac caacaaatgc gcaacctatg gcaatgacac
    10921 cgccaataac gctggcccag acggtgaaat caatccccgt tgacgggatg gtctgcatga
    10981 aggtgaaaat acttggcaaa ccaaaggagt aaactttcgt ttgcgcgtag ccaatgatgg
    11041 tggcccccaa tgctccactg atacaggcga taacaaaggg gtacttacgc ggcaggttga
    11101 cgccatatac cgctggttcg gtgataccaa acagacccgt caacgccgct gatcccgcca
    11161 ccactttttt ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacctgcg
    11221 ccataatggc gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat
    11281 tgatacacag cggcaccagg ccccagtgca gtccgaacat gacgaagatt tgccagaagc
    11341 cgcccattac cgcgcccgca aatgcaggaa ccgcctgata aagccagaga taaccggcgg
    11401 caatcatttc gcttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga
    11461 taaccatcag acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat
    11521 taagtcggcg ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg
    11581 acgagtaatt caacaatgtg accggaatac ccaggaaatc cagccccggc gcatccgctt
    11641 tttgcccgtt ctcgaaggca gtcagaatta atggatgcac taacgctcca ccaatcacca
    11701 tggcagtaaa tggattaccg ccgaagcgtt tgcccgcggt gtatcccagg attatcggga
    11761 agaacccaaa caaggcatca ctggcgctga ataaaattaa ataagtacca ctttgttcgg
    11821 tcgtccactg aaaagtgagc gccagagcca gcataccttt caggatcccg gttgccgcca
    11881 tcagaccgat cagaggcgta aaaatacctg aaataacata aacaaagcgg tttagcagat
    11941 tacctttctc atcattttcc ggtgcctgtt gcgctttttc gccaaggcct gccacgctgt
    12001 taaccgccag gaagacatcg gccacatggt tacctatgac cacctgaaac tgaccaccgc
    12061 tttccaccac cataataata ccgggggtct ttttcagtac ctcagcttgc gctttgcttt
    12121 catcctttaa cttaaaacgt aatcgcgttg cacaatgcat cagactcaca atgttatctg
    12181 cgcccccgac tcctgcgact atttttctgg ctaactccgt cataacttgc cctctaccgc
    12241 tttgcggcaa aactccaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt
    12301 tttttcaggt tttgcccgct tagtgcggta acaatccttt actcagtaat aatatttcag
    12361 tgttctttgc gcacgcgctc tatatttatg gctaaaaaca taatctctgc gggtgaaatt
    12421 ttacgttgat actgcaaacc aataaaaatg gcgatccgtt ccgcacattg ccatgcttgc
    12481 gggtaatttt gttttactgc ttgttgtaat gattcatcac tatcgttaat tgaagcatgt
    12541 tcaagaatac gccaggataa aaacttcaga tgtgtaacca gtcgctgata actcaagctt
    12601 tcttcctggt aattaaggct gaactgaaat tttattaatt gcagcatttc gcgcattaac
    12661 tgcgtgacac ctgcaacatc ctccatattt ccgctcattt gggcactgac cagatgcatg
    12721 gcaataaagc ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggtt
    12781 aacgcttctt cccctagctg gaactctttc gggtaaagcc gctggatatc ccacagcaac
    12841 gggttgggca gcaacacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt
    12901 agcgagatat aaatactgtc ctgtaatttt cccaagcgct cctgcgctaa agagataaga
    12961 cgatcacagg ttgccatcac ctcaagagga atatgactta agagttcgct taatcgcccg
    13021 ttcagttcat gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg
    13081 ccagcgcgtt tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca
    13141 tcaataacca ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg
    13201 acctttttta taaacaaaaa aacccgactt caccagtatt ctctggttat gtcaggtttt
    13261 gcctgcgaat gcagtaacaa tccagtcatt tattaatggt ttttataacg aacatccagg
    13321 ttcggaaatt aatttaattg cgtgcagaga aaatagcaat gcgctattga taaaaatatg
    13381 accatgctcg cagttattaa ctttgtgtaa ttttaggaat ttataaagtt atatataa
//
LOCUS       DADLTF010000112        12948 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.112, whole genome shotgun sequence.
ACCESSION   DADLTF010000112 DADLTF010000000
VERSION     DADLTF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12948
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.112"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(506..1597)
                     /gene="ychF"
                     /locus_tag="JN749_003016"
     CDS             complement(506..1597)
                     /gene="ychF"
                     /locus_tag="JN749_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAY7369350.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1714..2298)
                     /gene="pth"
                     /locus_tag="JN749_003017"
     CDS             complement(1714..2298)
                     /gene="pth"
                     /locus_tag="JN749_003017"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAY7369351.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2576..2854
                     /gene="ychH"
                     /locus_tag="JN749_003018"
     CDS             2576..2854
                     /gene="ychH"
                     /locus_tag="JN749_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HAY7369352.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="JN749_003019"
     CDS             complement(2909..4588)
                     /gene="dauA"
                     /locus_tag="JN749_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HAY7369353.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4713..5660)
                     /locus_tag="JN749_003020"
     CDS             complement(4713..5660)
                     /locus_tag="JN749_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HAY7369354.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="JN749_003021"
     CDS             complement(5811..6662)
                     /gene="ispE"
                     /locus_tag="JN749_003021"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAY7369355.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="JN749_003022"
     CDS             complement(6662..7285)
                     /gene="lolB"
                     /locus_tag="JN749_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HAY7369356.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPTNMELTDGGQRIKLKMDNWIVK"
     gene            7499..8755
                     /gene="hemA"
                     /locus_tag="JN749_003023"
     CDS             7499..8755
                     /gene="hemA"
                     /locus_tag="JN749_003023"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HAY7369357.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8797..9879
                     /gene="prfA"
                     /locus_tag="JN749_003024"
     CDS             8797..9879
                     /gene="prfA"
                     /locus_tag="JN749_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAY7369358.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9879..10712
                     /gene="prmC"
                     /locus_tag="JN749_003025"
     CDS             9879..10712
                     /gene="prmC"
                     /locus_tag="JN749_003025"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAY7369359.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10709..11101
                     /locus_tag="JN749_003026"
     CDS             10709..11101
                     /locus_tag="JN749_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HAY7369360.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11105..11914
                     /locus_tag="JN749_003027"
     CDS             11105..11914
                     /locus_tag="JN749_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HAY7369361.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11950..12804
                     /gene="kdsA"
                     /locus_tag="JN749_003028"
     CDS             11950..12804
                     /gene="kdsA"
                     /locus_tag="JN749_003028"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HAY7369362.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
BASE COUNT         3235 a         3256 c         3353 g         3104 t
ORIGIN      
        1 cgcttgatgt atctgcataa caaaaacaat atgcgccatt tttgcattac taataagaaa
       61 aagcaaaaaa catcgatatt catttttggt caatggattt tctggcctat cgttattatt
      121 gtgctactgg ttatcatttt catcttgcca ggatcgcacg taatcacgtt attcggggac
      181 tttatctgcg ggatattacc cttcaggtaa gtccttttct cgccagcata ttggagcgag
      241 atcaatattg atcagctgtg aagagggctg atgtgtagtg gaacaaaaac tgacgttata
      301 tcactgctaa tgagatatca gtcatctgaa cacttatgtc tttaattatt ctcgtggttc
      361 actgtaggca ataagcacaa aagtgtagga tgttacaaga atgattagga ctcggtgaaa
      421 tgaaaaatcc acgcaattgc gtggatttta tatacttttg cgctcttcat gagatttagc
      481 gaaacctcat gagacaataa attaattaga cgttgaaaag gaagttcatc acatcgccat
      541 ctttcacgat gtaatcttta ccttctgcac gcattttgcc tgcttctttc gcgccttgtt
      601 cacctttgta agtgatgaaa tcttcaaacg agatggtttg tgcacggata aagccttttt
      661 caaaatcagt atggattttg cccgctgcct gcggcgcggt tgctccaacc ggaatggtcc
      721 atgcacgcac ttctttcacc ccagcggtga agtaagtttg caggttcagc agcttataac
      781 cggcacggat cacacggttc aggcccggtt cttccagccc aagctcctgc ataaactcgt
      841 cacgttcttc gtcgtccagt tcggcaatgt ctgcttcaac agcagcacaa accggaacca
      901 caacagaacc ttctttcgcc gcgatttcac gcacctggtc aagatatggg ttgttttcaa
      961 aaccgtcttc gttgacgttg gctatgtaca ttgttggttt cagcgtcagg aagctcaggt
     1021 aacgaatagc cgctttctct tcagcgctta aatccagcgc gcgcagcata cctgcgtttt
     1081 ccagctgggg caggcatttt tccaggaccg ccagctcagc tttcgcgtct ttatcgccac
     1141 ctttggcttt cttctgtacg cgatgaatcg cacgttcgca ggtgtcgagg tctgccagcg
     1201 ccagttcggt gttgataact tcaatatcgt cagccgggtt aactttgccg gaaacgtgaa
     1261 tgatgttgtc attttcaaag cagcgaacaa cgtgaccgat cgcttcggtt tcacggatgt
     1321 tggtcaggaa ctggttaccc agaccttcgc ctttcgatgc gccttttacc agaccggcga
     1381 tatcgacaaa ttccatggtc gtgggaagcg tacgttgcgg ttttacgatt tcagccagtt
     1441 gatccaggcg agggtcaggc attggtacga cgcctgtgtt cggctcaatg gtgcagaatg
     1501 gaaagttggc cgcttcaata ccggctttgg tcagcgcgtt gaacagggta gatttcccga
     1561 cgttgggcaa accgacgata ccgcatttga atcccatgat ttaaatcacc ttaatatctt
     1621 aataatcaac ctgttattgc taacagattg cagaaatgga aataactttg cctattatac
     1681 acggcactcg gcaaaaatgc cgcagacaac gacttattgc gctttaaagg cgtgcaatcg
     1741 gttcgttgct ttggtcaagc catctgtaaa ccacatttca gtacaacgcg ccgcttcgtc
     1801 gatggcttca tcaattaact tctgttcact aacaggcggt ttgcctaaca caaaaccgac
     1861 aactttattt ttatcgcccg gatgaccgat tccgatgcgt aaacggtgaa agttagggtt
     1921 attacccaat ttactgatga tgtctttcag tccattgtga ccaccatggc caccgcccaa
     1981 cttaaatttg gcgacgccag gaggcagatc cagttcgtcg tgggccacca gaatttcgtc
     2041 cggattaatg cggaaaaaac tggccatcgc cgcaacggct ttgccgctga gattcataaa
     2101 tgtagtcggg actaacaggc ggacatcttc gcctccaagg gtgactcgcg aagtataacc
     2161 aaagaattta gcctcttcgc gcagcggagc gcgcaaacgc tctgccagta agtcaacgaa
     2221 ccaggcacca gcattatgtc gcgttgcggc gtattcagca ccggggttcg ccaggccgac
     2281 aatcaattta atcgtcacgt ttttttgtcc tgagtgtgta cataactggc gcgtagttta
     2341 ctggttgcgg ccccgcttga caaaaaactg cgtatcaaat gcagataacg taataattgc
     2401 ctgagtggac tattagaaag tcaaggtgtt caggcgttta tttgtaaagt tttgttgaaa
     2461 taagggttgt aattgtgatt acgcccgcac ataacccaca gggtgttgtc tatactttac
     2521 acataaggaa gaggggtatt ccctgttaca acccagaaag ttccggaggt gacatatgaa
     2581 acgcaaaaac gcttcgatac tcggtaacgt gctcatgggg ttgggtctgg tggtaatggt
     2641 ggtcggcgtg gggtattcaa tcctcaacca gttaccacag tttaatatgc cccagtattt
     2701 cgcacatggt gcagtgctaa gtattttcgt cggtgccatt ctctggctgg cgggtgcccg
     2761 tgttggcggg catgaacagg tgtgcgaccg ttactggtgg gttcgtcact atgacaaacg
     2821 ttgccgccgt agcgataatc gccgtcatag ctaacaaata atgcagtttg ctgaccagtc
     2881 agtttgcgct gactggtcaa tctcgtactt ataaatccgc catcgccgcg cgacgattcg
     2941 ggaagaacgc gaggcgtccc gggatcggtt gaatgcctgc gcgagccata gttcgcagtg
     3001 gctggaattc cacgttgcac acgcgcagtt cacatccttc gggcagacgc ttcacaaaac
     3061 gctggaacgc atcaagacca ccagcatcaa gtaccggaac ggcatcccac ttcagaatca
     3121 caatccgttt gccttcaaga cgtgactcca ggtccgtgaa taagccttca gcagcagcaa
     3181 aaaacagcgg gccaataacg cgcagaacca ggacatcgtc tggaacatct acgactaccg
     3241 gtgccaggcg agtcatacgt gcgatacgac gcataaacag cagcgatgcc agcacgatcc
     3301 ccacgctgat ggcaataacc atatcaaaca gcacggtcag tgacatacac agcagcatga
     3361 cgatgatgtc atctttcggc gcatgacgca gcaagtcgac cactttgtgc gcttcactca
     3421 tgttccacgc caccatcaac agcagggctg ccatggcgga aagcggcagc caggagagca
     3481 gtggtgccag taccagcagg gcgagaataa ccagaataga gtggatcacc gccgagatag
     3541 gggaagttgc cccggcacgg acgttagcgg cagaacgcgc gatggcagca gtagcggtaa
     3601 taccaccaaa gaatggagcg atgatattcc ccagcccctg accgaccagt tcactattcg
     3661 ccttgtgctt agtcccggtc ataccatcca gtaccacagc gcagagcaga gattcgatcg
     3721 ccccgagcat tgccattgag aatgccgcag gcagcagggt gcgaatagaa tcccaggtta
     3781 gcgtgaattc tgaattaggc agatcccacg gcagcaccag ttgcggcagc agttgcggaa
     3841 taccgttacc ctgagaacca tcggccagga cgtagtggaa ttgcgaaccg atggtagcaa
     3901 catgtccgcc gagcaggtta acaatcccca tcaccgcgca accagccagc aaggccggaa
     3961 ggtgaccggg taaacgaatg cccagacgcg gccagaaaac cagaatgcca agcgtcacaa
     4021 tgccaatggc agcatcaccc acattaatgg tcggcagcgc cataaataat gcgccgactt
     4081 tttgtagata atgttccggg acatgggcca tttgcagacc gagaaaatct ttaatctgca
     4141 tggtaccgat ggtgatcccg atacccgagg tgaaacctaa ggtgacggaa accggaatat
     4201 actcaatcag acgaccaaag cgtgccagcc ccatcagaat caaaaagatt cccgacaaca
     4261 aggtcgcaac cagcagtcct gccagtccaa actgttgcga aacgggatag agaattacca
     4321 caaatgccgc agtcggaccg gaaacgctaa agcgtgaccc acccgttaga gcaatgacaa
     4381 tccccgcaac agctgcggta tataaaccgt actggggtgc cacaccacta ccaatagcca
     4441 acgccatcgc cagcgggata gcaataatcc cgacggttat cccggcaatc aggtcacggg
     4501 taaaccgtgc ggcagtatat ttttctttcc agcaagcgtc gatcagagcg cggaaaggca
     4561 tcacatgtga ggaaaatatt ttgttcacaa taatgtttca tccgtgagcg catcatctgt
     4621 caactaaatg gcaggtgaag gaggcatagg tcatacaaat ggatattaca gacaaaaaaa
     4681 cccgccgcag cgggtctttg agccgggttc gattagtgtt cgaacatggc agagatcgat
     4741 tcttcgttgc tgatacgacg aatcgcttcg gccagcatac ctgacagggt cagagtacgc
     4801 acgttcggca gtgatttgat ttcatcgctc agcggaatgg tatcgcagac aacgacttca
     4861 tcaattacag agttacgcag gttgttcgcc gcgttgccag agaagatcgg gtgagtcgcg
     4921 tacgcaaata cacgtttagc accacgttct ttcagcgctt cagcagcttt acacagcgta
     4981 ccgccagtgt cgatcatatc atcgaccagt acgcagtcac gacctgcaac gtcaccgatg
     5041 atatgcatca cctgggaaac gttcgcacgc ggacgacgtt tgtcgatgat tgccatatcg
     5101 gtatcgttca gcagcttagc gatagcgcgg gcacgcacaa cgccgccgat gtccggagaa
     5161 accacaattg ggttatccag attcagctgc agcatgtctt ccagcaggat cgggctacca
     5221 aatacgttat caaccggaac gtcgaagaaa ccctgaatct gttcagcgtg cagatccact
     5281 gtcagcacac ggtcaacacc gacgctggag aggaagtctg caaccacttt cgcagtgatt
     5341 ggtacacgag cggaacggac gcgacggtcc tggcgcgcat agccaaagta ggggataaca
     5401 gcggtgatac gacctgcgga agcacgacgc agggcatcaa ccataacgac taattccatc
     5461 aggttgtcgt tagtaggggc acaagtggac tggatgatga aaatatcacc accgcgtaca
     5521 ttttcgttaa tttgtacgct gacttcgccg tcgctaaagc gacctacagc ggcgtcgccg
     5581 agtgaagtgt acaggcggtt ggcaatacgt tgtgctagta ccggggtggc gttaccagca
     5641 aaaagcttca tatcaggcac gagaagaacc tcaggcatgc gtccattggt ggaaagaatc
     5701 tgccgaaaac tgtgcgggcc aggcatgatc ctttccaggc ggtgtattaa agagcgcgat
     5761 gcaacgtctg gaacaaggtg acgttgtcac cgaaactcag cttgcccggc ttaaagcatg
     5821 gctctgtgca atggggaaag attaacgcct ttcgccacaa agccattgag ccattccggg
     5881 gcttgctcta gcacctggcg ggcttcagac tctgtatcaa attcagcaaa gacacaggcc
     5941 cctgtcccag tcaggcgcga cggggcgtat tttaacagcc aggaaagcac cgcatcaacc
     6001 tcgcgaaaac gttttcttgc gataacctcg caatcattgc tgaattcaca ttttagcaac
     6061 gtttctattg acctttttgg cgtattgcgc gggagttcag gatctttaaa aatcaccgga
     6121 gtcggaatac ttacaccagg gtgcgccacc agataccact tctctggcgg atccaccggc
     6181 gttagtattt caccaacgcc ttcggcaaac gcggcatgcc cccgaacaaa gacaggaaca
     6241 tctgcgccca gcgtcagccc catttccgcc agctcatcca tgcttagccc gcattgccag
     6301 agatgattta atgccaccag gaccgtcgcg gcattggatg aaccaccgcc gagaccgccg
     6361 cccatcggca aacgcttgtc aatgctgata ttcgcaccgc ttcccgtcgg aagacgcccg
     6421 ctgtctgccg cagttttcat caacaaccgc gctgcgcgaa cgatcaggtt atcttcatgt
     6481 tccacgcctt caacgggcgt taacagacga atatccccat cgtcacgaag ctcaatgctg
     6541 atggtgtcgc cgtaatcaag aaactgaaac agcgtttgca gcgtgtggta accatccgca
     6601 cgctgaccgg taatgtataa aaacagatta agttttgccg gagagggcca ctgtgtccgc
     6661 attatttcac tatccagtta tccattttta acttgatgcg ttgaccaccg tcggtgagtt
     6721 ccatattggt tggcatcgca ggttgcgttt tggtgtcata accaccataa acaaccttcc
     6781 agtttttgcc attctggctg taggtaattt cgctcaggcg gtactggtcg tccagtttgt
     6841 agtcggttgc atcacccggt aaacctaaaa tccactggcg caagctgttg agcggaattg
     6901 gcattccggt caatttgcca atcatctctt cggcgtcatc ggcggtataa cgctgacctt
     6961 tattgtcgac taactgcacg ttacccggtt gagcattcag ctccagttcc gtgctgccca
     7021 atgggttagt gagcagcaga cggtagcgat cctggccggt ttgctgccag aaaaagcggg
     7081 cgtacacttt ttgttggtca gaaatataag cgaacgcgcc gcgagtctga tactgattaa
     7141 gattgcgcac gtcttgctga tgctgacgcc attgtggcga atccgggctt ttgccaggac
     7201 ctttgggcgt ggtaacggaa caggcagtga gcacaagagc agccagcggt agcaggcgga
     7261 taagacgaaa atcgggcagg ggcatagtga tgacaagtcc ttgagatacg ttgcagttat
     7321 aacccttaat gctagcgtta ccgtccgcta tcgtctatgt tcaagttgtc ttaattgcca
     7381 gaatctaacg gctttcggca attactccaa aagggggcgc tctcttttat tgatcttacg
     7441 catcctgtat gatgcaagca gactaaccct atcaacgttg gtattatttc ccgcagacat
     7501 gaccctttta gcactcggta tcaaccataa aacggcacct gtatcgctgc gagaacgtgt
     7561 atcgttttcg ccggataagc tcgatcaggc gcttgacagc ctgcttgcgc agccgatggt
     7621 gcagggcggc gtggtgctgt cgacgtgcaa ccgcacggaa ctttatctta gcgttgaaga
     7681 gcaggataac ctgcaagagg cgttaatccg ctggctttgc gattatcaca atcttaatga
     7741 agaagatctg cgtaaaaacc tctactggca tcaggataac gacgcggtta gccatttaat
     7801 gcgtgttgcc agcggcctgg attcactggt tcttggggag ccgcagatcc tcggtcaggt
     7861 taaaaaagcg tttgccgatt cgcaaaaagg tcatatgaag gccagcgaac tggaacgcat
     7921 gttccagaaa tctttctctg tagcgaaacg cgttcgcact gaaacagata tcggtgccag
     7981 tgctgtgtct gtcgcttttg cggcttgtac gctggcgcgg cagatctttg aatcgctctc
     8041 tacggtcaca gtgttgctgg taggcgcggg cgaaaccatc gagctggtag cgcgtcatct
     8101 gcgcgaacat aaagtacaga agatgattat cgccaaccgc actcgcgaac gtgcccaaat
     8161 tctggcagat gaagttggcg cggaagtgat tgccctgagt gagatcgacg aacgtctgcg
     8221 cgaagccgat atcatcatca gttccaccgc cagcccgtta ccgattatcg ggaaaggcat
     8281 ggtggagcgc gcattaaaaa gtcgtcgtaa ccaaccaatg ctgttggtgg atattgccgt
     8341 tccgcgcgat gttgagccgg aagttggcaa actggcgaat gcttatcttt atagtgtgga
     8401 cgatctgcaa agcatcattt cgcacaacct ggcgcagcgt aaagccgcag cggttgaggc
     8461 ggaaactatt gtcgctcagg aaaccagcga atttatggcg tggctgcgag cacaaagcgc
     8521 cagcgaaacc attcgcgagt atcgcagcca ggcagagcac gttcgcgatg agttaaccgc
     8581 caaagcgtta gcggcccttg agcagggcgg cgacgcgcaa gccattatgc aggatctggc
     8641 atggaaactg actaaccgct tgatccatgc gccaacgaaa tcacttcaac aggccgcccg
     8701 tgacggggat aacgaacgcc tgaatattct gcgcgacagc ctcgggctgg agtagcagta
     8761 catcattttc tttttttaca gggtgcattt acgcctatga agccttctat cgttgccaaa
     8821 ctggaagccc tgcatgaacg ccatgaagaa gttcaggcgt tgctgggtga cgcgcaaact
     8881 atcgccgacc aggaacgttt tcgcgcatta tcacgcgaat atgcgcagtt aagtgatgtt
     8941 tcgcgctgtt ttaccgactg gcaacaggtt caggaagata tcgaaaccgc acagatgatg
     9001 ctcgacgatc ctgaaatgcg tgagatggcg caggatgaac tgcgcgaagc taaagaaaaa
     9061 agcgagcaac tggaacagca attacaggtt ctattactac caaaagatcc tgatgacgaa
     9121 cgtaacgcct tcctcgaagt ccgtgccgga accggcggcg acgaagcagc gctgtttgca
     9181 ggcgatctgt tccgtatgta cagccgttac gccgaagccc gccgctggcg ggtagaaatc
     9241 atgagcgcca gcgagggtga acatggtggt tataaagaga tcatcgccaa aattagcggt
     9301 gatggtgtgt atggtcgtct gaaatttgaa tccggcggtc atcgcgtgca acgtgttcct
     9361 gctacggaat cgcagggtcg tattcatact tctgcttgta ccgttgcggt aatgccagaa
     9421 ctgcctgacg cagaactgcc ggacatcaac ccagcagatt tacgcattga tactttccgc
     9481 tcgtcagggg cgggtggtca gcacgttaac accaccgatt cggcaattcg tattactcac
     9541 ttgccgaccg ggattgttgt tgaatgtcag gacgaacgtt cacaacataa aaacaaagcg
     9601 aaggcgcttt cagtgctcgg tgctcgcatc cacgctgctg aaatggcaaa acgccaacag
     9661 gccgaagcgt ctacccgtcg taacctgctg gggagtggcg atcgcagcga ccgtaaccgt
     9721 acttacaatt tcccgcaggg gcgcgttacc gatcaccgca tcaacctgac gctctaccgc
     9781 ctggatgaag tgatggaagg taagctggat atgctgattg aaccgattat ccaggaacat
     9841 caggccgacc aactggcggc gttgtccgag caggaataat ggaatatcaa cactggttac
     9901 gtgaagcaat aagccaactt caggcgagcg aaagcccgcg gcgtgatgct gaaatcctgc
     9961 tggagcatgt taccggcaga gggcgtactt ttattctcgc ctttggtgaa acgcagctga
    10021 ctgacgaaca atgtcagcaa cttgatgcgc tactgacacg tcgtcgcgat ggtgaaccca
    10081 ttgctcattt aaccggggtg cgagaattct ggtcgttgcc gttatttgtt tctccagcga
    10141 ccttaattcc gcgcccggat acggagtgtc tggtggagca ggcactggcg cggttgcctg
    10201 aacaaccttg ccgtattctc gatctcggga cggggaccgg ggcgattgcg cttgcgctgg
    10261 ctagcgagcg cccggactgc gaaattatcg ctgtagatcg tatgcctgat gctgtctccc
    10321 tggcacaacg taatgcccag catctggcga tcaaaaatat ccacattctg caaagcgact
    10381 ggtttagcgc gctagccggg cagcagtttg cgatgattgt cagcaatccg ccgtatattg
    10441 acgagcagga cccacatctt caacaaggcg atgtccgctt tgagccgctc actgcgctgg
    10501 ttgcggcaga cagtggaatg gcagacatcg tgcatatcat cgaacagtcg cgtaacgcgc
    10561 tggtatccgg cggctttctg cttctggaac atggctggca gcagggcgaa gcggtgcgac
    10621 aagcatttat cctcgcgggg tatcatgacg tcgaaacctg ccgtgactat ggtgataacg
    10681 agcgcgtaac gctcggccgc tattatcaat gacaagtttt tctacactgc ttagtgttca
    10741 tcttattagt atcgcgcttt ctgttgggct attaacctta cgtttctggc tacgttatca
    10801 gaagcatcca caggcatttg ctcgctggac gcgcattgtg ccgccggttg tcgatacggt
    10861 gttattgtta agcggcattg cgttgatggc taaagcgcac atcctgccat tttccgggca
    10921 ggcacagtgg ctgactgaaa agctgtttgg agttatcatt tatatcgttt tgggttttat
    10981 tgcactcgat tatcgtcgta tgcacagtca gcaggcgcgc attattgcct tcccgctggc
    11041 gttggtggtg ctgtacatca tcattaaact cgccaccaca aaagtaccgt tactggggta
    11101 agttatgaga tcgttagctg atttcgaatt taataaagcg ccattgtgcg aaggcatgat
    11161 cctggcttgc gaagcaatcc gccgcgattt tccctcgcaa gatgtttacg acgaactgga
    11221 gcgtctcgtt agtctggcga aggaagaaat cagccagctt ctgcctttag aagagcagct
    11281 ggaaaaactg atcgcgctgt tttacggcga ctggggattt aaagcctcac gcggcgttta
    11341 tcgtctttcc gatgcattat ggctggacca ggtgttaaag aatcgacagg gcagtgcggt
    11401 atcattaggt gcggttttat tatgggtcgc gaatcgtctc gatttgccgc tgctgccggt
    11461 gattttccct acgcagctga tattgcgcat tgaatgtccg gatggcgaaa tttggctgat
    11521 taatcctttt aacggtgaat cgttaagcga acatatgctg gacgtatggt taaagggaaa
    11581 tatcagcccg tcggcggaac tgttttatga agaccttgat gaagctgata acattgaggt
    11641 aatccgcaaa ttgctggata cactcaaagc ctcgttgatg gaagaaaatc agatggagct
    11701 ggcgttacgc accagcgaag ctttattaca attcaaccct gaagatccct atgaaattcg
    11761 cgatcgcggg ttgatttatg cgcaactgga ttgcgaacac gttgcgttga acgatttaag
    11821 ttatttcgtt gaacagtgtc cggaagaccc gatcagcgaa atgatccgtg cgcaaataaa
    11881 taacatcgcg cataaacata ttgtgctgca ttaattaatc gacattttac tcaagattaa
    11941 ggcgatccta tgaaacaaaa agtggttagc attggcgaca tcaacgtagc aaatgacctg
    12001 ccgttcgtac tgtttggcgg tatgaacgtg ctggaatctc gcgatctggc gatgcgcatt
    12061 tgcgagcact acgtaactgt gacccagaaa ctgggtatcc cttacgtgtt caaagcctct
    12121 tttgacaaag ccaaccgctc ctccatccac tcttatcgtg gaccgggcct ggaagaaggg
    12181 atgaaaatct tccaggagtt gaagcagact tttggcgtga aaattatcac cgacgttcac
    12241 gaaccaagtc aggcacagcc cgttgctgat gtcgtggatg tgattcagtt gccggcgttt
    12301 cttgctcgcc agactgacct ggttgaagcc atggcgaaaa ccggtgcggt aattaacgtc
    12361 aagaaaccac agtttgtcag cccggggcag atgggtaata tcgttgataa attcaaagaa
    12421 ggcggcaacg aaaaagtgat tctttgcgat cgtggtgcta acttcggcta tgacaacctg
    12481 gttgtcgata tgctgggctt cagcattatg aagaaagtgt ctggtaactc gccggtgatt
    12541 ttcgacgtga cccacgcact gcaatgccgc gatccgtttg gcgcagcttc cggtggtcgt
    12601 cgtgctcagg tggctgagct ggcacgagcc ggtatggcgg taggtctggc ggggctgttt
    12661 attgaagcgc atccggatcc ggaacatgcg aaatgtgatg gtccatccgc gctgccgctg
    12721 gctaaactgg aaccgttcct caagcagatg aaagcgattg atgatctggt gaaaggtttc
    12781 gaagaactgg ataccagcaa gtaatctttt ttgcttgaaa aataaagtct tagcgttctg
    12841 cgttaagact tttttcatgg gtgccagata caaaaaaggc cgcaggctgt tacccctgcg
    12901 gccggtttcg ggcgcatatt gccatcacgg cagcctgacg cccgtttt
//
LOCUS       DADLTF010000113        12786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.113, whole genome shotgun sequence.
ACCESSION   DADLTF010000113 DADLTF010000000
VERSION     DADLTF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.113"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(2..361)
                     /gene="frdD"
                     /locus_tag="JN749_003029"
     CDS             complement(2..361)
                     /gene="frdD"
                     /locus_tag="JN749_003029"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HAY7369363.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(372..767)
                     /gene="frdC"
                     /locus_tag="JN749_003030"
     CDS             complement(372..767)
                     /gene="frdC"
                     /locus_tag="JN749_003030"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HAY7369364.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(778..1512)
                     /locus_tag="JN749_003031"
     CDS             complement(778..1512)
                     /locus_tag="JN749_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HAY7369365.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(1505..3313)
                     /gene="frdA"
                     /locus_tag="JN749_003032"
     CDS             complement(1505..3313)
                     /gene="frdA"
                     /locus_tag="JN749_003032"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HAY7369366.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3638..4615
                     /gene="epmA"
                     /locus_tag="JN749_003033"
     CDS             3638..4615
                     /gene="epmA"
                     /locus_tag="JN749_003033"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HAY7369367.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            4834..6336
                     /gene="yjeM"
                     /locus_tag="JN749_003034"
     CDS             4834..6336
                     /gene="yjeM"
                     /locus_tag="JN749_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HAY7369368.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(6487..9798)
                     /gene="mscM"
                     /locus_tag="JN749_003035"
     CDS             complement(6487..9798)
                     /gene="mscM"
                     /locus_tag="JN749_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HAY7369369.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSIAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(9820..10788)
                     /gene="psd"
                     /locus_tag="JN749_003036"
     CDS             complement(9820..10788)
                     /gene="psd"
                     /locus_tag="JN749_003036"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HAY7369370.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(10885..11937)
                     /gene="rsgA"
                     /locus_tag="JN749_003037"
     CDS             complement(10885..11937)
                     /gene="rsgA"
                     /locus_tag="JN749_003037"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HAY7369371.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNIKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDG"
     gene            12032..12577
                     /gene="orn"
                     /locus_tag="JN749_003038"
     CDS             12032..12577
                     /gene="orn"
                     /locus_tag="JN749_003038"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HAY7369372.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         2905 a         3334 c         3393 g         3154 t
ORIGIN      
        1 gttagattgt aacgacacca atcagcgtga caactgtcag gatagcagcc agaccgtaga
       61 aaacccattt gcccgcaggc acgtggattt tcagatcgtg catcgcgtgg tgcatacggt
      121 gtaaaccaca ccacagcggc agaacgatca tcaggaacag gaatacgcga ccaatgaagc
      181 tctgcgcgaa cgccagaacg cgctcgtagc tcagcgcatc acccggaaac agccccagtg
      241 gcagcagaat acccaccagc aggatcatca ccggcgcaat aattgcgctc cacataccac
      301 cggccccgaa gaggccccag aataccggtt cgtcagaacg ctttggattt ggattaatca
      361 tctcaggctc cttaccagta cagggcaaca aacaggatta cgatggtggc aaccacagtt
      421 accgcccaga gacttttgat aattggctct ggtcccattt tttcgtcttt tacaatgata
      481 ttggccgctt tcggtgccag ttcaaaccag gttttggtgt gcagcagagc tgccgtcaga
      541 gtgatcaggt taatgatcac gataaccggg ttttgtaaaa agtcgacgaa tcccgcccag
      601 gcttccgggc catttttcag ggcaaacagc ccgaaaatca gttcaatgct gaaccacaca
      661 gccggaaccg ccgtgccttc gcgcagcatg taaaagcgat aaaacggcaa ttttttccac
      721 caggtggacg tcattggccg tacatacggt ttacgtttag tcgtcatgtt gcactcctta
      781 gcgtggtttc agggtcgcga taagaaagtc tttcgaactt tctactttgc cctgctgaat
      841 ggccgcagcc ggatcgacgt gtttcgggca gacttcggag caatagccca cgaaagtaca
      901 gctccataca ccgttctggc tgttcaactg cgccatacgc tccttcttac cgtggtcgcg
      961 gctatcttcg ttataacgat gcgccagcgt aatggcagcc ggaccgatga actctgggtt
     1021 caggccaaac tgcgggcacg cggcgtagca cagaccacag ttgatgcaac cggagaactg
     1081 gtgatacttt gccatctgcg ccggggtctg gatgttagta ccctgatccg cggtgcggga
     1141 gttgccgatg atgtacggtt tgatcgcttc cagactttcg atgaagtggg tcatatcgac
     1201 caccagatcg cgttcaatcg ggaagttagc taacgcttca accttcatac cgtcggtgta
     1261 atcacgcagg aaggttttac atgccagttt tggcacgttg ttaaccatca tgccgcagga
     1321 accacaaatc gccatacggc aggaccagcg gtaactcagg tccggtgcca ggttgtcttt
     1381 gatgtagccc agcgcatcca gtaatgaggt agttgcgtca taaggcactt catagaatgc
     1441 gctatgcggt gcggtatcga cttccgggtt atagcgcacc acctcaattt tcaggttttt
     1501 catctcagcc attcgccttc tccttattat tggctgcttc cgccttatcg gctgcatccg
     1561 cttcgccacc gtaaacgcgt ttagctggcg gcagcgtagt aatcttcacg tcgctgtact
     1621 ccaggcgagt cgtgccatca gcatcgcgga aggcgagggt gtgtttgaga aagttgacgt
     1681 cgtcacgctc ggtgcaacct tcgtccagac gctggtgtgc gccgcgggac tctttacgtg
     1741 ccattgcgga gtgcgccata cattcagcaa cgttcagacc gtggctcagt tcaatggtgt
     1801 agagcaggtc ggtgttgaac acgctggagg tgtcggtgat gcgcacgcgc ttgaagcgtt
     1861 cctgcagctc tgccagcttg tcgatggttt tctgcatcag ttccggcgta cggtagatac
     1921 cgcaaccttc ttccattgcc aggcccattt cgtcgcggat cttcgcccag ttttcgccgc
     1981 catcctggtt aaccagatct ttcagacgtt gttcaacgcc agctgcctgc gcttcaattg
     2041 ccgcttcgtt gccattaccg gcagttgctg cacgctctgt cgcttgttca ccggccagac
     2101 ggccgaagac caccagttcc gccagggagt tggagcccag acggtttgca ccgtgcagac
     2161 caacagagga acattcaccc acggcgaaca gacctttaat gcgggtttca cagttctgat
     2221 cggtttcgat accgcccatg gtgtagtgtg cagtcggacg taccggaatc ggttctttaa
     2281 ccggatcgac gccaacgtac gctttcgcca gttcgcagat gaacggcaga cgttcatgca
     2341 gttttttctc gccgaggtga cgcaggtcga gataaaccac atcaccacgc ggcgtggaga
     2401 tggtgttgcc tttacgccat tcgtgccaga aggcctgaga aactttgtcg cgtggaccca
     2461 gttccatata tttgtttttc ggctcgccca gcggagtttc cgggcccatg ccgtaatctt
     2521 gcagataacg gtagccattt ttgttgacca gaataccgcc ttcaccacgg cagccttcgg
     2581 tcatcaggat accggaacct ggcagaccgg ttgggtgata ctgaacgaat tccatgtcac
     2641 gcagcggaac gccgtggctt agcgccatac ccataccgtc accggtaacg atgccgccgt
     2701 tggtgttgta acgataaacg cgacccgcac cgccggtagc cataacgacc gcgttagcac
     2761 ggatctgcac caacgtgcct tccatcatgt tcattgctac caggccgcga acatgaccat
     2821 catcaaccag aatatccagc acgaaatgtt cgtcaaaacg ctggatctgc gggaattgca
     2881 gagaggtctg gaacagcgtg tgcagcatat ggaagccggt cttatcggcg gcgaaccagg
     2941 tgcgctcgat tttcatgccg ccgaagcgac gtacgttgac gctaccatcc gggcgacggc
     3001 tccatgggca tccccacagt tccagttggg tcatttcggt tgggcagtgg tggacgaaat
     3061 aatccacgac atcctgctca cacaaccagt cgccacccgc tactgtatcg tgaaagtgat
     3121 attcgaagct gtcatgatcc tgcgcgacag cggcggagcc cccttctgca gcaacggtat
     3181 ggctacgcat cgggtatact tttgagatta gtgcgatttt tgcatttgga tttgcctgcg
     3241 cggcagcaat tgcagcacgt aatcccgcgc caccggcgcc tacaatggca agatcggctt
     3301 gaaaggtttg cacgacattc ctccagattg tttttatccc acagccccgt acttcagggt
     3361 aagtgcctga aagttacggt ctgcgaacgc tattccactg ctcctttata ggtacaacag
     3421 tatagtctga tggataagtc tgaaatttga cgagatcgat ttttttagtg cgcaagaggg
     3481 ctaaattatc actgaagatg attaatttaa ttactaaacc atcagatcgt gcttttttta
     3541 gtcactgccc accgctgttt gattcctgcg taaaatttgt ctcgccgtcg cgttgcgagt
     3601 agacttcgtg cccttgtcaa aaactggaga tttaactatg agcgaaacgg catcctggca
     3661 gccgagcgca tccattccta acttattaaa acgcgcggcg attatggcgg agatccgtcg
     3721 tttctttgcc gatcgtggag tgctggaggt ggagacgcct tgtatgagcc aggcgacggt
     3781 aaccgatatt catttggtcc cgtttgagac acgtttcgtt ggccccgggc attcgcaggg
     3841 gatgaatctc tggttaatga ccagcccgga ataccatatg aaacgcctgc tggttgctgg
     3901 ttgtgggccg gtattccagc tgtgccgcag cttccgtaat gaagagatgg ggcgttatca
     3961 caaccctgag ttcactatgc tggagtggta tcgaccgcac tatgatatgt accggttgat
     4021 gaacgaggtg gacgatctct tacaacaggt gctggactgc ccggcagcag aaagcctttc
     4081 ttatcaacaa gctttcttgc gttatctgga aattgacccg ctctctgccg acaaaacgca
     4141 actgcgggaa gtcgcagcga aactggattt gagcaatgtt gctgataccg aagaagaccg
     4201 cgacacgttg ctacaattgc tgtttacctt tggcgtagag ccaaatattg gtaaagaaaa
     4261 accgaccttt gtgtaccact ttccagccag ccaggcatca ctggcgcaaa tcagtaccga
     4321 agatcatcgg gtcgctgaac gctttgaggt ttattataaa ggtattgagc tggcgaatgg
     4381 tttccatgaa ttaacggatg cccgtgagca gcaacaacgc tttgaacaag ataaccgtaa
     4441 gcgcgcggcg cgcggtttgc cgcagcaccc cattgaccag aatctgattg aagccttgaa
     4501 agtcggtatg cctgactgct ccggcgtggc attaggtgtt gatcgtctgg tgatgttggc
     4561 gctgggcgcg gagacactgg ctgaagtcat cgcctttagc gttgaccggg cataattctg
     4621 aaaaattact gaattaacag cgaagaatgg cgtgataagc gccattctgt agcatatgtt
     4681 ttcatttttc cgttaaaggc cacaatttaa gactggatat ttgatatcat caaggtatca
     4741 attctgtatt ggtttttacg ctgcctgctc aatgttgcgc agtgcagttc agtgggcacg
     4801 tgttatacac gcgctgaaat gaaggatggt ttcatgcctc acacgataaa aaagatgagt
     4861 ctgataggac tcatattgat gatctttact tccgtatttg gatttgccaa tagcccatcg
     4921 gcttattact taatgggtta tagtgcgatt cccttttata tattttctgc attgttattc
     4981 tttattccat tcgccttaat gatggctgaa atgggagctg cttatcgcaa agaagaaggt
     5041 ggtatctatt cctggatgaa taatagtgtc ggaccacgtt ttgccttcat tggtacgttt
     5101 atgtggtttt cctcttatat catctggatg gtgagtacct ccgcgaaagt ttgggtaccg
     5161 ttctcaacat tcctctatgg tagtgacatg acccagcact ggcgtattgc cggactggag
     5221 cctacgcagg tggttggtct gctggcagta gcatggatga ttctggtcac cgtcgttgct
     5281 tcaaagggga ttaataaaat tgcccgcatt actgcggtgg gcggtattgc agtaatgtgt
     5341 ctgaatttag tattgctgtt agtaagcatt actattttgt tattaaatgg tgggcatttc
     5401 gcgcaggata ttaatttcct tgcatcaccg aacccgggtt atcagtccgg tctggcaatg
     5461 ctatcgtttg tggtatttgc tatttttgcc tatggcggaa ttgaagcggt tggtggtctg
     5521 gtcgataaaa cggaaaatcc agaaaagaac tttgccaaag gtattgtttt tgccgctatt
     5581 gttatttcaa tcggttattc gctggcaata tttttatggg gcgtcagcac aaactggcag
     5641 caggtattaa gtaatggttc cgttaacctt ggcaatatta cctatgtgct gatgaagagc
     5701 ctcggggtga cgctgggtaa cgcactgcat ttgtcacctg aagcgtcatt gtcgctgggc
     5761 gtatggtttg cgcgtattac cggactttcg atgttcctcg cttataccgg tgcgttcttt
     5821 acgctttgct attcaccgct gaaagccatc atccagggga cgccgaaagc gttgtggccg
     5881 gaaccgatga cgcgcctgaa tgcgatgggg atgccgtcta tcgccatgtg gatgcagtgc
     5941 gggttggtta ctgtcttcat tctgctggtt tcgtttggtg gcggtaccgc atcggcgttc
     6001 tttaacaagc tgacgctgat ggcgaacgtg tctatgacgc ttccttacct gttcctcgcg
     6061 ctggctttcc cgttttttaa agcacgtcag gatctcgaca gaccatttgt gattttcaaa
     6121 acgcgtatgt cggcaatgat tgcgacggtg gttgtcgttc tggtggtgac atttgcgaac
     6181 gtcttcacca ttattcagcc tgtggttgaa gccggagact gggacagcac attgtggatg
     6241 attggcggcc ctgtcttctt ctcgctgtta gcgatggcga tttaccagaa ctattgcagt
     6301 cgcatggcga ataaacctga gttagctctc gactgatcat atgaaatcct gcaactcatc
     6361 gtctctccgg cgatgagttg tcaggaagta atgcgtatgg atgatactct ccagggccgg
     6421 tgataactga agatgatgtt aagctgctaa atgagccacg ttgacatcat ttggcagctt
     6481 catgcattac aaacttcccg cctgacgacc tttgcccgca gacgtcagcg ttctccccgt
     6541 ttgtttaccg ttgaggcttt ccagacgcat ctggaagggc gggaatggca tatcgatacc
     6601 gtgggcgtgg aagcctgcca gaatcagctg gtggatctca tggcgtagcg gcatacggtg
     6661 acccatctca gcggcgtaaa tacgcagctc gaaaatctga atcccctgtt gcagatccac
     6721 caggaagact tccggtgccg ggttgtcgat caccagcgag cagcgacgcg ctgcggtgag
     6781 caggatttcc gtcacttctt cactgttggc atcggcaggg gccggaatcg tcaacactac
     6841 gcgcgtgacc gagtcagaga gcgaccagtt gataaactgc tcggtaataa acgccttgtt
     6901 cggcacgatt atctctttac ggtcccagtc gctgatggtg gtggcgcggg tgttaatttt
     6961 tgtcacgcta ccagtaagat cgcggatcgt taccgtatcg ccaatgcgaa tcggtttttc
     7021 gaacaggatg atcaggccag agataaagtt ggcgaaaatt tcctgcaaac caaaaccgag
     7081 accaacaccg agcgcggcaa ccagccactg caatttcgac cactcaatac caatcattga
     7141 gaagccgacc agcccgccaa tcagcatcag cagatatttt gtgatggtgg tgatggcgta
     7201 acccgtaccc ggcgttaaat ccaggtgctg caaaatcgcc agttccagca gcgcgggcag
     7261 gttgcgcacc agctgcgtgg tgatgataaa taccagaatg gcaatcagca ccgcaccgag
     7321 ggtaattggc tccagacttt ctacgccctg taccgtggag gtgacatccc acagcgaaat
     7381 attttcgagg aagccgaaag cggaatggat ttctgaccac agcacaatga ccgaaagcag
     7441 ggcgatcaac atcaaaattg agcgcaccag ccgcaaggat tgcgcactga tggcatcgag
     7501 atcgacttcg ctttcatcga cttcaattgc tccttccggg ctactgtgat gatgcgcttc
     7561 ctcttcgcca cgcgcacgtt gcgctaacat ctctgcccgg cgatgcttcg cccggtcaaa
     7621 cgccagcctg cgacgctgga tcagcatcca gcggcggata acgtgataaa ccaccagtag
     7681 caggaaccag atggcaatcg aggtttcaag cctcgctaac agtgcctgcg ccgttgccag
     7741 ataacccacc gccgacgcca gaatggcaac cagtggtgcg ccaatcatca tgttccacag
     7801 catatggttg gtaatgttgt cgccgctgcc ttctttgttg agatacaacg gaataccggc
     7861 ttttttcagg cttagggtga ccaccgccag cgcaccgcaa atgaggataa agcaaagccg
     7921 tcccagcgaa ccggaaaatt cgcggtcgtc gaggttatcg aacatcatca gcgccataat
     7981 cagcccgatg ctcatcaggt agtagcgcat cccacgggaa acacgttcgc gcggccagcc
     8041 aaaatgagcg ataaacaagc cgttcgggcg ggcaaaggtg gcgcaaatca tcactaccca
     8101 cagcagcggc acggtggccg ttacaccatc gccaatcgcg accgccagcg gataaggcca
     8161 cgcctcgcgc aagccgtaac ccagcgtcat ccacagcacc ggtaacggtg acgcgacgag
     8221 aatcgaccag aaaagagtgc gcaacgtcag ccagaagtga tcctgagtca ctttgccgac
     8281 tttcgccgcc gaacgttcaa gaaaacgggt gaaatagcgg cgcgagtaaa tactgcaacc
     8341 gaccagaatc aacgcgccaa acagcggcaa aattgtctct ttgctggtca gcatcatcac
     8401 actggctttg cccaactgac tgaaggtgtc cagcgaaatg agacgacgca gatcctgggc
     8461 gatttccagc ggccaggcga tggtcatcgg gcgcacgtca gaggtccaga acagatagcg
     8521 gtgcgttgct tcgttcacct ctttcagcgc atcctccagt tgcccgttgg agactttcag
     8581 cttggtcagt tccaatagta gcgtgtcgcc accctgcaac aatgagttca gcaactcacg
     8641 ctgagtgcgc agttgtgctt ccagaatacg gttttgctcg gcggtcagcg gctgaccgtc
     8701 ggcctgatgg atttgccgta gcagcggctg tttattaagt aaatcctcat aacgtaaccg
     8761 ttgcacacgc aactgcgcca tttcggtatc aagctgttgt ggttttggca tttctggtag
     8821 ccgtgccacc tgcgcccgca gcgcttcgcc gagcaggttg gacgatccca gccattgcga
     8881 ctgttcacgc agcgtattca acgcctgccg gacctgtaac gtctggctgg cagcctgacg
     8941 ctgttgcgag gcaacgagct ccatccgctg cgcctgttga ttcagcgctg ccgatagttc
     9001 gcggttaatt ttgaattgcg cgacgatatc tttcggcaaa tcggcactgt tttctgccag
     9061 taattcggta ctttccaacg cccgctccgc ctcaagctga cgttggctat tcaactgatt
     9121 acgtaagccc tgcaaatacg catccagttg ctggctctct ttttccgcca gctctgagcg
     9181 taagcgcgct aattcctggc ggttattggc agacagctgc gccagctcca gttcatcaac
     9241 gagcgcctta agacgtgcag agtcagactg caacgcgaaa ttttgtgcct gattgagcgg
     9301 agtattgccg gtaagcgttc ccaggcggcg ctcgatctca tttaactgac ggcgggcgtc
     9361 ggtttgctgt tgcggcagtt gattcagcga atcggcaatc tcgcgggcgc gctcctgctc
     9421 ttgctgggcc tgacggcttt tatccagcaa ctggctgctg acctggagaa tttcctgatt
     9481 cagcgcgtcg gtagacattc ccggcgacac gctgcgcggc tcgtcacgca tgttgtttaa
     9541 ttgtgcgcgc agagtagcgg agagtttcgg ataattatcg ataacttgct gatattgttt
     9601 gatgcgctca agggaacctt ttcgttcctc aagcgcattt aaggcagact ggagcgcctc
     9661 tacgacttcc ggctgtgcgg gtttcgccgc ttttgcctgc tccagttcct gagtgatttg
     9721 tttgctatcg ggggccgtcg cggcgtacgc cccccaactg aggcaccagg ccatcagaaa
     9781 agtgataatc aggcgcacgt cagcgtttcc tttgatggat tagacctggt cttttttatc
     9841 gtcaaccaat ggcctggcgt cgtgttctgc ttcgatctct tcagcaggaa gtggggcggg
     9901 ttcagcgtct ggcgtaacaa aggtttcggt agatactgcc agcggctggc caattttcgt
     9961 gacagacagg ctttccagtt gctcaaccag attcacttta cccggtgcaa acaggttgat
    10021 aacggtggaa ccgagtttaa agcgacccat ttcctggcct ttcagcagtg ccacagaacc
    10081 gtcgttttcc ccggcaggcc aggtccagcg cttgatgata ccttcgcgcg gcggcgtaat
    10141 ggtgcccgcc cagaccgtct caatgctgcc aacaatcgtc gctccgacca gaatctgcgc
    10201 cattgggcca aattcggtat cgaaaaggca aatcacgcgt tcgttacggg caaacagatt
    10261 cggcacgttc tgagcggtga gatggttaac ggaaaagaga tcgcccggca cgtagatcat
    10321 ctcacgcaga ataccgttgc acggcatgtg tacgcgatgg tagtcacgcg gggagaggta
    10381 agtggtcaca aacgtaccgt tgcggaacag gtccgccatc agatagttgc ctgccagcag
    10441 ggcttcgagg ctgtagttgt ggcctttggc ttgcaggatt ttatcttctt cgattttacc
    10501 caactggctg ataacgccat cggcaggcat gaccagtaca ttcggatcgg tatcgattgg
    10561 gcgtacttcg tcacgcagcg gacggacaaa gaattcgtta aaggtgcggt agctggcggt
    10621 gtccggcttt tgcgcctctt tcatgtcgac cttgtagtat ttaacgaaca gatcgataac
    10681 cagttttgtc agccatcctg cccgcttgct tgcgccccaa cccgccaggc gagtaagcca
    10741 tagtttcggc agaatgtact gtagcgaaag tttaaatgaa tttaacaagg tagcctccag
    10801 gccattgttt tgtcgttcct gatccggcca acatgccgga tcctgaaaaa aaggggacga
    10861 ttctaacgac ggttagctta attgtcagcc atccgtatca gaaaagtttt tacgcgtttt
    10921 tacctgcgcc atgctttcca gaatacggtg atagttttcg aaacgggttt ccgcgatttt
    10981 cccttcctca accgcttccc ggatagcgca gcccggatcg gtatcgtgtt tgcaatcgcg
    11041 atatttacac agacctaaat agtcatggaa ttcgacaaag ccctgagtga tttgttccgg
    11101 ctccaggtgc cagaggccga actcacgcac accaggggaa tcaatcacat caccgccgtg
    11161 cgggaagtga tacagccgag cggcggtcgt ggtgtgctga ccgagccccg agttgtcaga
    11221 gacatcgttg gtcaggatct ctttttgcag ccccagcaac gcgttcagca ggctggattt
    11281 accgacgcca gactgcccgg caaaaatgct gatgcgcccg gtcaacgcct cttccagcgg
    11341 tttcagccca tcctgagtat ggctggaaac catcaatacg cgataaccga tgttgcggta
    11401 gatatccatc tgctcgttga cgaacgccat gccttcgtcg tccagcaggt ctatcttgtt
    11461 gagcacgata atcggctcaa tctgcaaggt ttcgcaggcc accaggtaac ggtcgataat
    11521 attgagcgac agctccggca aaatggcgga gacaatgaca atctggtcga tgttggcggc
    11581 tatgggtttc acgccgtcgt agaaatccgg acgcgttaac accgaggtgc gctcatgcac
    11641 cgcttccacg atccctttga tatttacgcc ttccgccgcc ggtttacccg gacgccagac
    11701 tacgcggtcg ccggttacca gcgaacggat ggtacggcga atattgcagc ggtgaacgtc
    11761 gccatcggcg gattccacat cagcgtgcat accaaagcgg ctgatgacga taccttcatc
    11821 aggctcgcca aacagattgt cgtcgtagtc gggcttctcc ttagacgttt taagacgacg
    11881 ctggtgattg gcgttcacgc ggcgctgctg gcctttggag agtttatttt tactcaatcg
    11941 tactgactcc tggtcgcccc tgatgggcaa aacatctatg atacacgcaa ttgtggatca
    12001 atatagtcac tgtgaatggg tggaaaatag catgagtgcc aatgaaaaca acctgatttg
    12061 gatcgatctt gagatgaccg gtctggatcc cgagcgcgat cgcattattg agattgccac
    12121 gctggtgacc gatgccaatc tgaatattct ggcagaaggg ccgaccattg cagtacatca
    12181 gtccgatgaa cagctggcgc tgatggatga ctggaacgtg cgcacccata ccgccagcgg
    12241 gctggtagag cgcgtgaaag cgagcacgat gggcgatcgg gaagctgaac tggcaacgct
    12301 cgaattctta aaacagtggg tgcctgcggg aaaatcgccg atttgcggta acagcatcgg
    12361 acaggaccgt cgtttcctgt ttaaatacat gccggagctg gaagtctact tccactaccg
    12421 ttatctcgat gtcagcaccc tgaaagagct ggcgcgccgc tggaagccgg aaattctgga
    12481 tggttttacc aagcagggga cgcatcaggc gatggatgat atccgtgaat cggtggcgga
    12541 gctggcttac taccgcgagc attttatcaa gctgtaaaat ttttcctggt cacgtaagcg
    12601 ccggaatcca cttgcggcgg cgtgattttg acgctaaatc gaacgtcttg ctgataattt
    12661 cagcacttga gataaaaacg caaaaaaaac ttttttgggg ggttgcagag ggaaagattt
    12721 ctcgtataat gcgcctcccg taacgacgca gaaatgcgaa aattacgaaa gcaaaattaa
    12781 gtagta
//
LOCUS       DADLTF010000114        12584 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.114, whole genome shotgun sequence.
ACCESSION   DADLTF010000114 DADLTF010000000
VERSION     DADLTF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.114"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(66..2015)
                     /locus_tag="JN749_003039"
     CDS             complement(66..2015)
                     /locus_tag="JN749_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7369373.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2100..2436)
                     /locus_tag="JN749_003040"
                     /pseudo
     CDS             complement(2100..2436)
                     /locus_tag="JN749_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2436..2792)
                     /locus_tag="JN749_003041"
     CDS             complement(2436..2792)
                     /locus_tag="JN749_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369374.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2792..4288)
                     /locus_tag="JN749_003042"
     CDS             complement(2792..4288)
                     /locus_tag="JN749_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369375.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAH
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4285..4446)
                     /locus_tag="JN749_003043"
     CDS             complement(4285..4446)
                     /locus_tag="JN749_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HAY7369376.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4456..5019)
                     /locus_tag="JN749_003044"
     CDS             complement(4456..5019)
                     /locus_tag="JN749_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369377.1"
                     /translation="MKLTTIIAALRERCPRFEERVGGAAQFKAIPDAGKLRLPAAYVV
                     PSDDAPGEQKSQTDYWQDLTEGFSVIVVLSNERDEKGQWAAYDAVHDVRRELWKALLG
                     WMPDPQGGEIVYVGGTLLDLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLS
                     IDVDYIDPGTGPDGDIEHHLEMRFPQN"
     gene            complement(5016..5540)
                     /locus_tag="JN749_003045"
     CDS             complement(5016..5540)
                     /locus_tag="JN749_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369378.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRDARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="JN749_003046"
     CDS             complement(5506..5922)
                     /locus_tag="JN749_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAY7369379.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="JN749_003047"
     CDS             complement(5919..6242)
                     /locus_tag="JN749_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAY7369380.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..8017)
                     /locus_tag="JN749_003048"
     CDS             complement(6287..8017)
                     /locus_tag="JN749_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAY7369381.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGHGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNGEVETLNGK
                     LTELENLKSDLEAELAEVKRPAGGTQNKVAGEHKEAFIGFMRKGREDGLRELERKALQ
                     VGNDEDGGYAIPEELDRTILTLLKDEVVMRQEATVITLSGSDYKKLVNLGGTTSGWVG
                     ETEARPETATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEDWINSELALEFAEQEE
                     IAFTSGDGSKKPKGFLAYESTDEDDKTRAFGKLQHIASGAASGVTADAIIKLIYTLRK
                     AHRSGAKFMMNNSSLFAIRLLKDNDGNYLWRPGIELGQPSSLAGYGIVENEQMPDIAA
                     DAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLMKI
                     GAATRQKAAA"
     gene            complement(7995..9236)
                     /locus_tag="JN749_003049"
     CDS             complement(7995..9236)
                     /locus_tag="JN749_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAY7369382.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="JN749_003050"
     CDS             complement(9236..9418)
                     /locus_tag="JN749_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369383.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="JN749_003051"
     CDS             complement(9430..11187)
                     /locus_tag="JN749_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAY7369384.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSAGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="JN749_003052"
     CDS             complement(11187..11669)
                     /locus_tag="JN749_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAY7369385.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="JN749_003053"
     CDS             complement(11817..12167)
                     /locus_tag="JN749_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7369386.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..>12584)
                     /locus_tag="JN749_003054"
     CDS             complement(12210..>12584)
                     /locus_tag="JN749_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7369387.1"
                     /translation="ITYKEQRDKNARELKLANATITDMQQRQRDVNALDAKYTKELAD
                     AKAENETLRADVAAGRRRLHIKAVCQSVREATTASGVDNATSPRLADTAERDYFTLRE
                     RLMTMQMQLEGAQEYIRTQCIK"
BASE COUNT         2827 a         3733 c         2919 g         3105 t
ORIGIN      
        1 aacaaactcc attgttagca tgttacgcga taattttgcc tcatctttca ccacaaaaaa
       61 agaactcacc ttttaaatgg agaataccca acatcatggg tgattttcat aaagggatcg
      121 cctgttttcg gcaaatcgat aacgcgcatt ccaggcggcg cgttgtcaaa cgtcacctta
      181 agttcgctgc gggttgccgg tgaagatgca aggcttaaca gctgattcct ttccagtgat
      241 acattcgggg tgtagcgttt atcgggagat aaatacccat caccacgtgg catctgccat
      301 ccggtcatgt catagacaaa atcatggaac tgtttgcccc actcgtcgaa cttatcattc
      361 agaccaaatc cactgttcag tgcatcagcc acaaaatttt taacaagcca gggatgttct
      421 ttttcaaact cctgcgccat catgcccagt tcaagcaatc caccaatcag actgatcttt
      481 ccgaggccct ttaatttcag ccctgttttt cctgccgccg ttgtccattg attctgggcg
      541 actgttgccg cacgaatact tgccaccgcc ttcacaccaa tatacagggc agaaaccgtt
      601 gctattgttt ttactgcctg ttcccatccc cccattgctc tgacaacctg atcaacctcc
      661 tgccagactt ttttcacaac cggaccgacc gtttcccagt tatcaattat gagatatgca
      721 ccaccgacga gaagcgcaat taatcccttt gccggcgtca tattcatcac gctacccatg
      781 atttttgtta tgcgtgtcaa tgtgccaatc gccacgccca tcgtcagtaa tgccgcacca
      841 gttttcgcga tggtttttac gacctcagga ttttctctga caaacgtgcg cacttcttcc
      901 agaaaaggct tcatctcttt tatgccttca ttgagtgaag gcaggaaggt ttcccccagc
      961 gtggaagaaa tcgcattgat ctggttctgc agcagcaaca actgattttc tgtcgtcgcg
     1021 gcacgggcgg catattcttt ctgcattgag ccgccatact gctgcgcatc cgccacacga
     1081 ttaaagttgg ttcgcaacaa atccagattc gtgagcagcg gcgcaatggc ccccagggat
     1141 tccttcccga acagcgcttt cagcacagca gcctgttttt ctttcggcac ttttgccaga
     1201 gaatccagta cgtgcagcat ggccccgcgg gcgtctttct gcatatccgc cgccagtttt
     1261 ttcgggctaa tgcgcaacga gcggagcact tttttctgcg atttggtggc agaatccctc
     1321 gctgtcagcg acaacataaa attctttatc cccgtcgcag caatttctga ctccaccccc
     1381 attccggcaa tggttgcccc cattgcggca atctctccgg aggccacacc cgcaacactg
     1441 cctaaagggc caatacgggt cacaacatca gaaatctttt tcgcacttgc aggaccggta
     1501 ttgccaagat agttaatctt atccgcaagc cctgccacct ctccctgtgt cagtttaaag
     1561 gcagtgcgcc actgcgccat catctgaccg gattcttctg ccgtggcgtc aaaggccacg
     1621 cccatcttca cggcatcgtc agtaaactgc atcagctcgt cacgggcgat accagcctga
     1681 ccacccgccg ccacaatttc ggcgatacct tccgccgaca tgggcagttc tgttgacagg
     1741 tcgcgcacct gctccgtcat tgccttaaac gcttccggcg tatccagacc atccaccact
     1801 ttccggacat ccgccatttt tgattcaagg gcaatggcag atttgaccgg gagcgccagc
     1861 gcccccagta ctgcggttcc ggcacccgct gcgcccagtg acaggctggc aaactccttt
     1921 ttaaaacctt tcagctggcg ctgcattccc ttcagcggtg ccgatacctt atccacggca
     1981 gtgatgatcg ccttcagctg aaaactgtca gccatgcttc atctcctcgt taatgcggac
     2041 ggcctcggct tccagttctg taaactgcga aacagccacc cgtcgcagct ccagtggatt
     2101 cagtttccag aaccaggcaa cattgtagag tcgtttcccg gaggtgcttc ccgtctccga
     2161 ctgggtaaaa aaacgcagga tctgcatgct ggtcttaaaa atatccagtt ttgccatctg
     2221 cgccgccgat gaacgcggga tccccgccag caacgggata tatttcagtg ccacctggct
     2281 gtccagttta atactgcctt caccggaaat aatgaaagga aaacccagcg cctcgatttc
     2341 gtcatacgtg ggctcgcgca actccagcac atgcagcatt tcgttatgcg ctgtcaccgg
     2401 tttttttaaa actatttctg taacgttcat tgccattact gataccctcc ctcttcgccg
     2461 tggaattcaa gatctgccgt gccttcttcg gcattatgat tagcctctcc gtgcagccat
     2521 gccgccgaaa gcacatacac catgccgttt gccagctcag cggtaatcgt catctggtct
     2581 gaggtggtaa ttttgtcgac cggaaagttt ttgggcactt taaacgtgcc cttaacgtaa
     2641 ggtgaccgcc aggtctcctt gtaatccaca tcccctgcca tgccgacgat gtcatcccgg
     2701 acattggtgt tcatcggcac ttcaatgcca cctgtcagcg ataactgctg accatccact
     2761 ttaaaaaaac aggttccggc gatctttgcc attatgctga ctcctctgaa tactgaagac
     2821 ggaactggtt aaccaccgca aagacacgta actgattaac ataatccggc gggaacagcg
     2881 tattcagtcg gttcgggtta tctgcatcac gctcaactat cagatactgt ttaaacagat
     2941 cgtaattttc cacaataccg gcacgctcca tctgacgata tgtcgccaga agttcccctt
     3001 tgataacggc aggagtgaca atcgcctgcc ccggaccaaa acgggtacca tcatttgcca
     3061 gcttgtgacg tccgtacttg cttgtgatga ccgatttcag tttgcgaaga acatgtgcac
     3121 tggtatgcag agtttcactg tccagatagc tgttgtctgc cacgccatac gcattctttt
     3181 tgtaggtggt tacggaacgc tggatctgca acgtgccgcc ttccacataa gccgtcgcca
     3241 cgccgtgaga taaaagggtc tgctgctctg tcatgatgaa tcgcttacct ttcggtgccg
     3301 gaagcatccc caccagctcc cccgtctgtg tcggacgggc aggatcattc cggataaata
     3361 ccgcttcacg ggcaaggcga ctggcaacca gttcatcgac aggcgactgg gtttcttttt
     3421 cgtaaccggc aagcgtgata tgttgctgat tatgcatatc tccggcacca accagctctg
     3481 acagcgttcc cagttttgcg gtatagacat gcccgtataa ctggcgcgca taactccagc
     3541 gaccgctgct gtcattcatt tcggtcatca tcatattgat ggaggcggca tcgttgaacg
     3601 gcagaccgat aaagtcgaat gcctcatcgc ccatagcagc aacagcggcg gtaagatcag
     3661 gcgctccgct accggcagca ccgacttccg tcaccacccg aagccctgct ggcagaatct
     3721 caccaccacc agaaccataa taattcagac agaccggtaa ctcattacca tacagcccct
     3781 tatgacgggc agtcagcgtc accacgcctg catcagatga cgccgtaaac ggcagggtga
     3841 taaccccgtt taccgcttcc ttaatcgcgg tggcaaccgc agtggcatca tcgccattca
     3901 ccacaggcac ctgtacacgg gagcgcccga catacagact cagggtgccg ctttcctctg
     3961 cttctccggc aaccgtcacc ctgaccgtcg ccgccgcccc tctggcttcc ggtactgcaa
     4021 taacatacag ttcaccgaaa ggatctgtct gacggtagac ttccaccata cgcgccagct
     4081 ggctccccgc cccacaaatc tggcgggcat aatctgccga tggcatcagc accaggctgt
     4141 taacctcaat ggctgcatcg ttgctggcat gcccgatcag taatgcaggc gcgctggtca
     4201 ccgctgtatt tgccgcagaa ttgttcatct cggcgtagaa caacggcacc agcgtattcg
     4261 acggaacagt attaaaactt atcgtcatga ttttttagcc ttatcctgtt taacacgtac
     4321 cacatcaccc gccgctatac ggcggaacca gtagctgctt tcttccacat ttcgcccttc
     4381 ggaaggcaaa aggtctccac gggcagggtc aggaaccgac cgccctttca ggggtttcac
     4441 aaacatgagg gactcttaat tctgagggaa acgcatttcc agatggtgct caatgtcacc
     4501 atccgggcca gtaccaggat cgatgtaatc cacatcaata ctcagcaggg ataaatccgg
     4561 cagggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcatca cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttccgg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccactcagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgccca ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacgtcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat tccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgctgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccgctcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcgcccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtctctgc tgcatgtcag taatggttgc gttcgccagc
    12541 ttcagttctc ttgcgttttt gtcgcgctgc tctttgtagg taat
//
LOCUS       DADLTF010000115        12427 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.115, whole genome shotgun sequence.
ACCESSION   DADLTF010000115 DADLTF010000000
VERSION     DADLTF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.115"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JN749_003055"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="JN749_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HAY7369388.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JN749_003056"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="JN749_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HAY7369389.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JN749_003057"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="JN749_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HAY7369390.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JN749_003058"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="JN749_003058"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HAY7369391.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="JN749_003059"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="JN749_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HAY7369392.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="JN749_003060"
     CDS             complement(8217..9110)
                     /locus_tag="JN749_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HAY7369393.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JN749_003061"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="JN749_003061"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HAY7369394.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /locus_tag="JN749_003062"
     CDS             complement(10007..10189)
                     /locus_tag="JN749_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HAY7369395.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="JN749_003063"
     CDS             complement(10241..11473)
                     /locus_tag="JN749_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAY7369396.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..>12427)
                     /gene="lpxK"
                     /locus_tag="JN749_003064"
     CDS             complement(11510..>12427)
                     /gene="lpxK"
                     /locus_tag="JN749_003064"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HAY7369397.1"
                     /translation="GLVSGAIRLCYKLKLKRAWRAPVPVVVVGNLTAGGNGKTPVVVW
                     LVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTTTAQAGDEPVLIYQRTDAPVAVSP
                     VRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVEIVVIDGVRRFGNGWWLPAGPMRE
                     RAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNLRTGTRCDVAQLEHVVAMAGIGHP
                     PRFFATLKMCGVQPEKCVPLADHQSLNHADVSALVSAGQTLVMTEKDAVKCRAFAEEN
                     WWYLPVDAQLSGDEPAKLLTQLTSLASGN"
BASE COUNT         2867 a         3538 c         3084 g         2938 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gactcggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagacc
//
LOCUS       DADLTF010000116        12312 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.116, whole genome shotgun sequence.
ACCESSION   DADLTF010000116 DADLTF010000000
VERSION     DADLTF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.116"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..490
                     /locus_tag="JN749_003065"
     CDS             <1..490
                     /locus_tag="JN749_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369398.1"
                     /translation="VSVATRQVAGNTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTA
                     RIRVNRGNLPAIKLNTTRRRAGEGLRVGKYFFRGAFVQQLANGRWHVLRCLPEARFAT
                     GHDHQGRLRKNRLPVEVVKIPMSGPLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHL
                     SK"
     gene            487..886
                     /locus_tag="JN749_003066"
                     /pseudo
     CDS             487..886
                     /locus_tag="JN749_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            897..1637
                     /locus_tag="JN749_003067"
     CDS             897..1637
                     /locus_tag="JN749_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369399.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1696..2082
                     /locus_tag="JN749_003068"
     CDS             1696..2082
                     /locus_tag="JN749_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HAY7369400.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2091..2420
                     /locus_tag="JN749_003069"
     CDS             2091..2420
                     /locus_tag="JN749_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HAY7369401.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2392..5331
                     /locus_tag="JN749_003070"
                     /pseudo
     CDS             2392..5331
                     /locus_tag="JN749_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5331..5660
                     /locus_tag="JN749_003071"
     CDS             5331..5660
                     /locus_tag="JN749_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369402.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5660..6358
                     /locus_tag="JN749_003072"
     CDS             5660..6358
                     /locus_tag="JN749_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HAY7369403.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYDRFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6363..7106
                     /locus_tag="JN749_003073"
     CDS             6363..7106
                     /locus_tag="JN749_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369404.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7043..7644
                     /locus_tag="JN749_003074"
                     /pseudo
     CDS             7043..7644
                     /locus_tag="JN749_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            7705..11184
                     /locus_tag="JN749_003075"
     CDS             7705..11184
                     /locus_tag="JN749_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HAY7369405.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLERWDTPKVVKGVSFLLRLTVAADDGSERLVSTGRTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDYAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKTLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTTNKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            11252..11851
                     /locus_tag="JN749_003076"
     CDS             11252..11851
                     /locus_tag="JN749_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HAY7369406.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11915..>12312
                     /locus_tag="JN749_003077"
     CDS             11915..>12312
                     /locus_tag="JN749_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005047577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369407.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPWYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAA"
BASE COUNT         2884 a         2921 c         3940 g         2567 t
ORIGIN      
        1 ggtctcggtt gccacccggc aggttgccgg gaataccgtt gcgggcgata accaggtgaa
       61 agggatcccc ctgaaactgg tacgtcagcg tgtccgggtg tttaaagcca gtccgtcagg
      121 aaaaatgacg gccaggatcc gcgttaaccg gggcaatctg cccgccatca aactgaacac
      181 aacacggcgg cgtgctggtg aaggactgag agtgggaaaa tactttttcc ggggggcatt
      241 tgttcagcaa ctggcgaatg gccgctggca tgttctgagg tgtcttcctg aagcgcgttt
      301 tgcaacaggg catgaccatc agggcaggct aagaaaaaat cgtcttcctg tggaggtagt
      361 gaaaatcccg atgtccggac cgctgacaca ggcatttgaa gatgcccgcg atcgcatcat
      421 tgctgcggaa atgccgaaac agctggggta tgccctgaaa caacaactga ggttacatct
      481 gagtaaatga accggcacac acaaatccgt cagagcgtgc tggcacgcct tcgggaacag
      541 tgtggagaca gcgccacgtt ttttgacggg cttccggcat ttattgatgc gcaaactgcc
      601 tgccgtggcg gtgtggctga gtgatgctca gtacaccgga aaaatgacgg atggagatga
      661 ctggcaggct gttctgtata ttgccgtctt catccgggca caggcaccgg attcagagct
      721 ggatatgtgg atggagagca ccattttccc ggccctgaat gatgtaccgg cactttccgg
      781 actcatcgac accctgaacc cactcggttt taactatcaa cgtgataatg agatggctac
      841 ctgggcgatg gcggaaatca cgtaccagat cacgtacacg aattaaggag gtggtaatga
      901 ccacaccaaa tccactggca aaaacgaaag gtgcgggaac gacgttatgg atgtataccg
      961 gcaacggcga cgcatttgcg aaccctttgt cggacactga ctggctgcgt cttgcgatgg
     1021 tgaaggatct gcaacctggc gagatgaccg ctgatgcaga agatgacact tatctcgatg
     1081 atgaagatgc agactggaaa acgacaaccc aggggcagaa atccgtcggt gatacttcgg
     1141 cgacgctggc ctggcgtccg ggtgacagcg ggcagaaaaa actggttcag ttgttcgatt
     1201 ccggtgaagt ctgcgcgttt cgtatcaaat accccaacgg tacggttgat gttttccgtg
     1261 gctggctgag ttcactgggt aaaaccatta cctcaaaaga ggtgatgacc cgtactgtaa
     1321 aaatcagcgg tgtggggcgt ccgtatctgg cagaggaggg cgctgaaacc gtgggtgtta
     1381 ccggactgac ggtggcaccg gcatctgtca gtgtcaaagc gggagcaacc accacactga
     1441 cctttacagt aaaacctgac ggggccagtg acaaagcgat cagtgtgcat tcgtcagatc
     1501 ctcagactgc ttcggtgccc ctgagcgggc ttgtggccac ggtgaaaggc gtgcagcagg
     1561 gcagtgtcag catcgtgggc atgacctctg acggagagtt tgtggcagtg gctgcggtga
     1621 ccgtcagcgc accataacag gacgatactc atcattgccc cggttatccg gggctttttg
     1681 catccggaga acatgatgtt tctgaaacag gatacgttta attatgaaaa acagtccgtg
     1741 gtgctcagtg agctgtccgg gctgcagaga attgaatatc tgacgtttgt tcagcagcga
     1801 aaggcaaagt ttgatgcaca ggagggagaa ctgccggagg ctgaacgaca gattgctttt
     1861 ctgcgtatgg gaatggatat caatgcctgg ctggtttccc gctcactgtg gaatgctgag
     1921 cagtctcagg atgttgagac gctttgcgca tccattatga caacatggtc gtatgatgcg
     1981 ctgggcgcgg gggcggagag ggttctgtcg ctgagcggta tggggaccat tgagaatgcc
     2041 ggggatgatg atcatgaggc gctgacgccg gaaaagtcct gacgcgggaa atgcagtttg
     2101 tcatgcggct tgcccgggag ttccggcggg cagactggcg gcagatgctg tcggatatgt
     2161 cggccactga gcttggtgag tggggcgatt atttccggat gcagagcttc agtgatgtgt
     2221 ggatggatgc gcagtttgcc tcgctgaagg cattgatcgt gagaatggtg tccggcagca
     2281 gcgatgctgc ggtggctgat ttcagccttt taccggaaga gaacgggata ccggagcgaa
     2341 cggacgaaga actgatgcat cttggggaag gtatttccgg aggtgtgcgt tatggaccag
     2401 atagccaacc tggtcattga tttggggatt gatgcggcag agtttaaaaa tgaaattccc
     2461 cgtatcaaaa accttctgaa tggtgcagcc agcgatgcag aacggtcttc tgcccgtatg
     2521 cagcgtttta tggagcgtca gactcaggcg gcccggcaga caatgcaggc ggcttcttcg
     2581 gctgcaacag cagcatcagc ccatgcgcag acggtggaga agaatgcacg ggctcatgaa
     2641 cgcatggccc gtgaggtgga acaaacccgt ctgcgcgtgg atgccctgaa tcagaaaatg
     2701 cgcgaggaac aggcgcaggc cagggcactg gcggaggcgc aggataaagc ggctgccgcc
     2761 ttttatcgcc agattgacag tgtgaaacag gccggtgcgg ggcttcagga attacagcgt
     2821 attcagcagc agatccgaca ggccagaaac agtggcgggg ttggtcagca ggattatctg
     2881 gcgctgattt cggagatcac ggcgaaaacc cgtgccctga cgcaggcaga ggaacaggcc
     2941 acccggcaga aagcggcgtt tatccgccag cttaaagagc aggcaacccg ccagaatctg
     3001 tcgtcttctg agttgcttcg cgccagggcg gctcagctgg gggtgttaat cagtcagatg
     3061 gcgcgcggca attttggtgc gctgagggga tccgggataa cgctggctaa ccgtgccgga
     3121 tggacaggcg cactgatgtc gccgaaaggc atgatgactg gcggcgttat tggcggactt
     3181 gtcgcggcgg tcctgggtct gggtaaagcc tggcatgacg gcggaaggag ggcgaggaat
     3241 ttaaccgtca gctggcgctg acgggacatt atgccggtgt cactgtcggg cagttgtgga
     3301 aactcagccg ggccatatcc gggaatggta tcacgcaaca tgcggcagcc ggtgcgctgg
     3361 cacaggtggt gggcagtggg gcatttcatg gaaacgatat tggtatggtg gcgaaagctg
     3421 ccgcacagat ggagcgatcg gttggccagt cggtcagcga taccataaat cagtttaagc
     3481 ggctgaagga tgatcctgta aatgccgcga aggcgctgga caatgcgctg cattttttga
     3541 ctgccactca gcttgagcag atacgcgtcc ttggggagca ggggcggtcc agtgatgcgg
     3601 cccggatcgc catgtctgcg ctggcagagg aaaccggtaa acgcacatct gatattgata
     3661 ataatctcaa tgcgctgggt agtacgctgc aaaccttgtc tgactggtgg aagcagttct
     3721 gggatgcggc catgaatatt ggtcgtgaag actcgctgga tgcgcagatt gatgcgttac
     3781 aggaaaaaat tcagcgcgcg aaaaaatatc cgtggacaaa cgcctccaca caggtggagt
     3841 acgatcagca gcgtcttaac gatcttcagg agaaaaaacg ccggaaggat ttgcaggatg
     3901 caaaagcgca ggcagaacgg aattaccagg agcaacagaa acgccggaat gctgaaaatg
     3961 ccgcgctgaa ccggatgaat gaaacggaag ctgcacgaca tcagcgggaa attgcgcgta
     4021 ttaatgccat gcagtacgcc gaccaggctg tcagggatgc ggcgatacag cgtgaaaatg
     4081 aacgttacga aaaagccatt aagaaaaata cacgggcaac ccgtaatgat gaggccaccc
     4141 ggttattgct gcagtacagt cagcagcagg cacaggtgga agggcagatt gctgctgcca
     4201 gacagtcagc aggcattgcc actgaaagga tgactgaagc gcataaacag cttctggccc
     4261 tgcagcagcg catcagcgac ctggacggga aaaagctgac ggcagatgaa aagagtgtgc
     4321 tggcccgtaa gaatgagctg attcaggcgc tgacgctgct ggatgtgaaa cagcaggagc
     4381 tgcagaaaca gacagcgctt aacgacctga gaaaaaaaac ggttcagctg accagccagc
     4441 tggcagacaa agaacgtgca ctgcgtgagc agcacaatct ggatattgcc actgcaggta
     4501 tgggggataa gcagcggcag cgctaccagg cacagttgcg catccggcag gaataccggc
     4561 aacagttgca acagcttgag aatgacagtc gccagaaagg cacttacggg acggaggact
     4621 accggagggc tgaggaggtg ctgaagggga gcctgaagcg acaactgaat gaaaacaaac
     4681 gctactggca ggaactggaa gtggcgcagg gcgactggaa aaacggtgcc atgcgggcgt
     4741 ttcagaattt tacggcggat gcggataatg cggcgggaac ggcagaacag atgtttacag
     4801 tggcattcag cagtgccggt aatgcactgg cgacattctg taccaccggt aagctgaatt
     4861 ttaaatcctt cacctcttcc ctgttgtcag atatggccag aattatggca cagatggcca
     4921 tgatgcaggc ggtaaagggc gtcggttctt tattcggctt cacgactaat gctgatggcg
     4981 gtgtttacca gtctgctgat ttgagtcgct acagtggcac ggtggttaac cgtccgacgt
     5041 tttttgcttt tgcaaaaggc gcgggtgtga tgggggaagc gggacctgaa gccattctgc
     5101 cactgcgtcg cggtgctgat ggtaagctgg ggggttgtgg cggatactgg tggttcaggc
     5161 atggtgatgt ttgccccgca gtacaacatt gagatcaata acgacggcac gaacgggcag
     5221 ataggtccgg ctgccctgaa ggtggtttat gacctcggga aaaaagcggc cgcggacttt
     5281 atgcaacagc aggcccgtga tggtggccgg ttaagtggag cataccggta atggagacgt
     5341 ttcactggaa agtgcgcccg gatatgaatg tggtatcaga gccgaaagtg gtgacagtga
     5401 agctgggcga tggttatgaa cagcgtcgtg cggcgggact gaataaccag ttgtcgactt
     5461 acagcgtgac gatacgtgtt cgtaaaggtg aacacccatc tttaaaagcc tttctggaac
     5521 ggcacggtgg cgtccgcgca tttcagtgga cgccacctta tgactggaaa ctgatcaggg
     5581 aggtctgtcg taaatggtcg gcaagcgtgg gggcgttatg ggtgactgta acggccgatt
     5641 ttgaacaggt ggtgaactga tgcaggatat ccggcaggaa acactgaatg aatgcacccg
     5701 tgcggagcag tcagcccggg tggagctctg ggaaatcgat ctgacagagg tcggtggtga
     5761 gcgttatttt ttctgtaatg agcagaacga aaaaggtgag ccggtcacct ggcaggggcg
     5821 gcagtatcag gcatacccca ttcagggggc ggggtttgaa ctgaacggca ggggctgtgc
     5881 tgcccgtccg acactgacgg tctctaacct gcacggcatg gtcaccggga tggcggaaga
     5941 tctgcagagt ctggtcggcg gaacggtggt ccggcgtaag gtttacgacc gttttctgga
     6001 tgcggtgaac ttcgtcaacg gaaacagcga cgccgatccg gaacaggagg tgatcagccg
     6061 ctggcgcatc gagcagtgca gcgaactgag cgcggtcagt gcctcttttg tgctggccac
     6121 accaacggag acggatggcg cggttttccc ggggcgtatc atgctggcga atacctgtat
     6181 gtggacttac cgttctgatg agtgtggtta cacgggcagg gcagtggctg acgagttcga
     6241 caaaccaacg acggatatcc ggaaggacaa atgcagcaag tgtatgcgcg ggtgtgagtt
     6301 gcgcaacaat accggtaatt tcggcggttt cctttccatc aataaacttt ctcagtaaat
     6361 ccatgacaca gacagaatca gcgattctgg cgcacgcccg gcgatgtgcg ccagcggagt
     6421 cgtgtggatt cgtggtgaga acgccggagg gagacaggta tcttcccagc gagaatatct
     6481 ccggtgagcc ggaggaacgg ttccggatgg ctccggagga ctggctgcgg gcacaaatgc
     6541 agggtgagat tgtggcactg gtccacagtc atcccggtgg tctgccctgg ctgagtgagg
     6601 ctgaccggcg gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cggggggcga
     6661 ttcacaagtt ccgctgtgtg ccgcatctca ccgggcggcg ctttgagcac ggggtgacgg
     6721 actgttacac gctgttccgg gatgcttatc atctggcggg gattgagatg ccggattttc
     6781 accgcgggga tgactggtgg cgtcacggtc agaatctcta tctggataat ctggaggcca
     6841 cagggctgta tcaggtgccg ttgtcatcag cacaaccggg cgatgtgctg ctgtgctgtt
     6901 ttggttcatc ggtgccgaat catgccgcca tttactgcgg cgatggtgag ctgctgcacc
     6961 atattcctga acaactgagt aaacgagaga ggtataccga caaatggcag cgacgcacac
     7021 actccctctg gcgtcaccgg gaatggcacg catctgcctt tacggggatt tgcaacgatt
     7081 tggccgccgc atcgaccttc gtgtgaaaac gggggctgaa gccatccgcg cactggccac
     7141 acagctcccg gcgtttcgtc agaaactgag tgacggctgg tatcaggtgc gcattgccgg
     7201 gcgtgatgca ggtgaaaccg aattgtctgc ccgtcttaat gagccgctgg caaatggtgc
     7261 agtgatccac atagtaccgc gtctggtggg agctaaaagt ggcggtgtgt ttcaggcggt
     7321 gctgggggca gctgttatgg cggttgctat atggatgccg gggtaggaat tatggcgagt
     7381 aatctgctgt tttctctcgg tgccagtatg acgcttggcg gtgttgcaca gatgctggca
     7441 ccgaaagcca gaactccccg tacacagaca acggataacg gcaaacagaa cacctgtttc
     7501 tcctcactgg ataacatggt tgcccagggc aatgttctgc ctgttctgta cggtgaaatg
     7561 cgcgtggggt cgcgggtggt atctcaggag atcagcacgg cagacgaagg ggatggtggt
     7621 caggttgtgg tgattggtcg ctgatgcaaa atgtttcatg tgaaaccgcc tgcgggcggt
     7681 tttgtcgttt atggagcgtg aggaatgggt aaaggcagca gtaaggggca taccccgcgc
     7741 gaagcgaagg acaacctgaa atccacgcag ttgctgagtg tgattgatgc catcagcgaa
     7801 gggccggttg acggtccggt ggatggatta aaaagcgtgc tgctgaacgg tacgccggtc
     7861 ctggacagcg aggggaagac aaacttttcc ggtgttacgg tggtgttccg cgccggcgag
     7921 caggagcaga caccgccgga ggggtttgaa tcttccggct cagagactgt gctgggtacg
     7981 gaagtgaaat acgacacgcc gatcacccgg accatcacgt cggcaaacat tgatcgtctg
     8041 cgcctgacct tcggtgtgca ggcactggtg gaaaccacct caaaggggga ccggaatccg
     8101 tcggaagtcc gcctgctggt tcagatacag cgtaacggtg gctgggtgac ggaaaaagac
     8161 atcaccatta agggcaaaac cacttcgcag tatctggcct cggtggtggt gggtaacctg
     8221 ccgccgcgcc cgtttaatat ccggatgcgc aggatgacac cggacagcac cacagaccag
     8281 ctgcagaaca aaacgctctg gtcgtcgtac accgaaatca tcgatgtgaa acagtgctac
     8341 ccgaacacgg cgctggtcgg cgtgcaggtg gattcagagc agttcggtaa ccagcaggtg
     8401 agtcgcaatt atcatcttcg cgggcgcatt ctgcaggtgc cgtcgaacta taacccgcag
     8461 acgcggcaat acagcggtat ctgggacgga acgcttaagc cagcatacag caacaacatg
     8521 gcctggtgtc tgtgggacat gctcactcat ccgcgctacg gcatggggaa acgtcttggt
     8581 gcggcagatg tggacaaatg ggcgctgtat gtcatcggcc agtactgcga ccagtcagtg
     8641 ccggacggat ttggcggcac ggagccgcgc atcacctgta acgcttacct gaccacacag
     8701 cgtaaggcgt gggatgttct cagtgatttc tgctcggcga tgcgctgtat gccggtatgg
     8761 aacgggcaga cgctgacgtt cgtgcaggac cgaccgtcgg ataaagtgtg gacctataac
     8821 cgcagtaatg tggtgatgcc ggatgatggc gcgccgttcc gctacagctt cagcgccctg
     8881 aaggaccgcc ataatgtcgt tgaggtgaac tggattgatc cggacaacgg tcatgagacg
     8941 gcgacagagc ttgtggagga cacgcaggcc attgtccgtt acggtcgtaa cgtcacgaag
     9001 atggatgcct ttggctgtac cagccgggga caggcgcacc gcgccgggct gtggctgatt
     9061 aaaacggaac tgctggaaac gcagaccgtg gacttcagcg tcggcgcaga agggcttcgc
     9121 catgtaccgg gtgatgtcat tgaaatctgc gatgatgatt atgccggtat cagcaccggt
     9181 ggtcgcgtac tggcggtgaa cagccagacc cggacgctga cgctcgaccg tgaaatcacg
     9241 ctgccgtcct ccggtaccac gctgataagc ctggttgacg gaaatggcaa tccggtcagc
     9301 gtggaggttc agtccgtcac cgacggcgtg aaggtgaaag tgagccgggt tcctgacggc
     9361 gttgcagaat acagcgtgtg ggggctgaag ctgccgacgc tgcgccagcg cctgttccgc
     9421 tgcgtgagta tccgtgagaa cgacgacggc acgtatgcca tcaccgccgt gcagcatgtg
     9481 ccggaaaaag aggccatcgt ggataacggg gcacactttg acggtgaccg gcgcggcacg
     9541 gtgaatggtg tcacgccgcc agcggtgcag catctgaccg ccgaagtcac cgcagacagc
     9601 ggggaatatc aggtgctgga gcgatgggac acgccgaagg tggtgaaggg tgtgagcttc
     9661 ctgcttcgcc tgaccgtggc agcggacgac ggcagtgagc ggctggtcag cacaggcagg
     9721 acgacggaaa ccacataccg cttcaggcag ctggcgctgg ggaattacag tctgacagtc
     9781 cgggcggtaa atgcccgggg gcagcagggc gatccggcgt cggtatcgtt ccggattgcg
     9841 gcaccggcag cgcctgtcac tattgaactg ataccggggt attttcagat aacggtggtc
     9901 ccgaaactgg ctgtatatga cccgacggtg cagtttgagt tctggttttc ggaaaagcgg
     9961 attgcggata tcaggcaggt tgaaaccagc gcccgctatc ttggcacggc gctgtactgg
    10021 atagccgcca gtatcaatat caggccgggc catgattatt atttttacgt tcgcagtgtg
    10081 aacaccgttg gcaaatcggc atttgtggag gccgtcggtc gggcgagcga ttatgcggaa
    10141 ggttatctga gtttttataa agggttgatc aataaaacgc atctcggcaa ggagttgtgg
    10201 acgcagattg ataacggtca gcttgcgccg gacctgactg aaatcaggac gtccattacg
    10261 aatgtcagca atgaaatcac gcaaaccgtc aataaaacac tggaaaatca gagtgctgca
    10321 atccagcaga tacagaaagt tcaggttgat acaaataata acctgaacag catgtgggct
    10381 gtgaagctgc aacagatgaa ggacggacgc ctttatattg cgggtatcgg tgccggtatt
    10441 gagaatacgc cagcaggaat gcagagtcag gtgctgctgg cggcagacag gattgcgatg
    10501 attaatcctg cgaatggcaa cacaaagccg atgtttgttg gtcagggcga tcagatattc
    10561 atgaacgacg tgttcctgaa acgcctgacg gctccgacca ttaccagcgg cggtaatcct
    10621 ccggcatttt ccctgacacc tggcggacgg ctgacggcga aaaatgccga tatcagcggt
    10681 aacgtgaacg cgaactccgg gacgctcaac aacgtcacga ctaataagaa ctgtcgggtt
    10741 ctgggaaaat tgtccgcgaa ccagattgaa ggcgatctcg ttaaaacagt gggcaaacct
    10801 ttcccacggg actcccgggc accggagagg tggccatcag ggaccattac cgtcagggtt
    10861 tatgacgatc agccgtttga tcggcaaatt gttattcccg cggtggcgtt tcgcggtgct
    10921 aaacatgagc ggaagaataa caatatttat tcgtcatgcc gcctgatagt gaagaaaaac
    10981 ggtgctgaaa tttataaccg aacgaccctg gataatacgc tgatatatac gggtgttatt
    11041 gatatgcctg ccggtcacgg tcacatgacg ctggagtttt ctgtatcggc atggctggta
    11101 aatggctggt atcccacagc aagtatcagc gatttgctgg ttgttgtgat gaagaaagcc
    11161 actgcaggca tcacgattag ctgaatttta taacccagat acgggcacca gaaatggtgc
    11221 cttttttatt gcagaaaagc gagaggtaat tatgcgtaaa gtttgtgcag ccattttgtc
    11281 cgcagccatc tgtctggctg tatccggtgc gcctgcatgg gcgtctgaac atcagtccac
    11341 gctgagcgcc gggtatcttc agccccatac tgatatgcca ggcagcgatg acctgaaggg
    11401 cattaacgtg aaataccgtt atgagtttac ggacacgctg gggctggtga cgtcattcag
    11461 ctatgccaac gctgaagatg agcaaaaaac gcattacagc gatacccgct ggcatgagga
    11521 ttccgtgcgt aaccgctggt tcagcgtgat ggcggggccg tctgtgcgcg tgaatgaatg
    11581 gttcagcgcg tatgcgatgg cgggcgtggc ttacagccgt gtgtcgacct tctccgggga
    11641 ttatctccgc gtaactgaca acaaggggaa aacgcacgat gtgctgaccg ggagtgatga
    11701 caaccgccac agcaacacgt ctctggcgtg gggggctggt gtgcagttta acccgaccga
    11761 atccgtggcc attgaccttg cttatgaagg ttccggcagt ggcgactggc gcactgacgg
    11821 tttcattgtg ggtgtcggtt ataagttctg attagccagg taacacagtg ttatgacagc
    11881 ccgctggttc aggcgggctt tttgtggggt gaatatggca gtaaagattt caggtgtact
    11941 gaaagatggg gcgggtaaac ctgtcgtaaa ttgtgcgatt gaactgcggg ccagaagaac
    12001 cagtccgacc gttgtggcac acgttgttgc cacttgcgtg acggacaata acggtgctta
    12061 tgtgattgag gctgagccgt ggtattacga ggttgcgctt cactgtaacg gctggcagcc
    12121 aacccgtgtc ggggatattg atgtggcacc gactgatgca ccggggacac tgaacgcgtt
    12181 tctgaatgca ccaaaggatg gtgatttacg tccggaggtg atgaagcgct ttgaggaaat
    12241 ggtggcgcag gcgcagcaga gtgccggggc tgcagccgga aacgcacagc agacggcgca
    12301 ggatgtggcg gc
//
LOCUS       DADLTF010000117        12225 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.117, whole genome shotgun sequence.
ACCESSION   DADLTF010000117 DADLTF010000000
VERSION     DADLTF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.117"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(124..291)
                     /locus_tag="JN749_003078"
     CDS             complement(124..291)
                     /locus_tag="JN749_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369408.1"
                     /translation="MHFRVTGEWNGEPFDRVIEAEDINDCYNHWMIWAQIAHADVTNI
                     RIEELKEHHAA"
     gene            complement(479..1318)
                     /locus_tag="JN749_003079"
     CDS             complement(479..1318)
                     /locus_tag="JN749_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7369409.1"
                     /translation="MTTITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVAYIFKHPAGKLFWALTDESNKEQADVIPVYAAAPASVVPDNASEPLAYAYKELT
                     PEIMRNHLAVFERYGIAPNDSSTTIQALRIALDGIERSDAMLHGAEPVSQTYKLPPLS
                     SNEVNDAAWKLHNMLTEHGPLNGRQFNNLKGCFYEALKVAMRNYPVTPDGWISCSERM
                     PEDTKMLLAFSQGQIVAAYWNWVMSPIDYKKYRAFTYLSGNILDDVTHWMPLLEPPEQ
                     DGE"
     gene            complement(1320..2006)
                     /locus_tag="JN749_003080"
     CDS             complement(1320..2006)
                     /locus_tag="JN749_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7369410.1"
                     /translation="MSEINYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVAQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEGKDSKIANLTAERDALREGEMGDARYSNTRAAADIYFQLVEECEIPAGGSLV
                     EYVDDMREKLESAEKRIAELEEREILLPERSSMLHRTDFHEDYQTVMAYKVSEVIDAI
                     RATGIRIKGE"
     gene            complement(1993..2307)
                     /locus_tag="JN749_003081"
     CDS             complement(1993..2307)
                     /locus_tag="JN749_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369411.1"
                     /translation="MTITKQRVEKIIYRHEMGLSSDVTAEEVYDLAVLALNLSNIANL
                     KRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLESPAKEATSE
                     RN"
     gene            complement(2304..2789)
                     /locus_tag="JN749_003082"
     CDS             complement(2304..2789)
                     /locus_tag="JN749_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369412.1"
                     /translation="MKQMTLIEMDGFLKGKCIPSDLKVNETNAEYLVRKFAEAEAKCA
                     ALAAENAGLKAALNHEVIPDTAVEAFNETAIIDHDWNDTSEWSWVENDTDVIRAVLEA
                     IKPETPATDAFLAEIERKAIRKFINSIEHILRDKLSPYDTEEMLETMRIFLEEQGGEQ
                     K"
     gene            complement(2786..2950)
                     /locus_tag="JN749_003083"
     CDS             complement(2786..2950)
                     /locus_tag="JN749_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7369413.1"
                     /translation="MRGLAYNLGILPAEMIIRQRVKPMPSREELLKRNSFPSVNQNKY
                     LNAMWRNGKK"
     gene            complement(2961..3254)
                     /locus_tag="JN749_003084"
     CDS             complement(2961..3254)
                     /locus_tag="JN749_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HAY7369414.1"
                     /translation="MPAPQYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="JN749_003085"
     CDS             complement(3268..3774)
                     /gene="ssb"
                     /locus_tag="JN749_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAY7369415.1"
                     /translation="MASRGVNKVIILGRVGQDPEVRYSPSGTAFANLTIATSEQWRDK
                     NTGEQKELTEWHRVAVSGKLAEVVGQYVKKGDQIYFEGMLRTRKWKDQSGQDRYTTEV
                     HVGINGVMQMLGGIGDSKQQAASRQSQKPQQQSSPAQHNEPPMDFDDDIPFAPVTLPF
                     PRHAIHAI"
     gene            complement(3775..4482)
                     /locus_tag="JN749_003086"
     CDS             complement(3775..4482)
                     /locus_tag="JN749_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HAY7369416.1"
                     /translation="MDLNKFDEPFSPEDIEWRIQQSGKTRDGKVWAMVLAYVTNRAIM
                     KRLDDVCGKAGWRNEYRDIPNNGGVECGISIRIDSEWVTKWDAAENTQVEAVKGGRSG
                     AMKRAAVQWGIGRYLYNLEEGFAQTSLDKKHGWHRAKLKDGTGFYWLPPSLPGWAIPA
                     SDNKPSPENTNQKSPSVDCEQILKDFSDYASTETDKKKLIERYQRDWQLMAGNEEAQA
                     KCVQVMNIRVNELKQAA"
     gene            complement(4737..4889)
                     /locus_tag="JN749_003087"
     CDS             complement(4737..4889)
                     /locus_tag="JN749_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HAY7369417.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PWYRRK"
     gene            complement(4874..5005)
                     /locus_tag="JN749_003088"
     CDS             complement(4874..5005)
                     /locus_tag="JN749_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HAY7369418.1"
                     /translation="MIYAIAGGARMGAFQLNESLLERITRKLRDGWKRVDVLLCAMK"
     gene            complement(5030..5998)
                     /locus_tag="JN749_003089"
     CDS             complement(5030..5998)
                     /locus_tag="JN749_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein TolA"
                     /protein_id="HAY7369419.1"
                     /translation="MSEVTDLVVIEKANAMTVFQSADQIEEILQKVEREVMSFVPDIT
                     TAKGRKEIASLAYKVAQTKTYLDGLGKDLVAELKEIPKLIDANRKTVHDRLDELKAKA
                     RQPLTDYEEEQARIKAEEEAKAAAEALAKQIESDHEIAILMDREFDRQREEARLKAEQ
                     EKREHEERLKREAEEKARAEAEAKAKAEIEAAARREAEAKAAAERAKRERIEAEQRAQ
                     REAKEAAERAEREKQAAIEAERRKAQEEAERIRREAEAKEQARIAEEKRIKDEEERRA
                     KDKAHRKEVNNKILADLIKVGASEDVAKNIITAIVKGEVFATKITY"
     gene            complement(6166..6408)
                     /locus_tag="JN749_003090"
     CDS             complement(6166..6408)
                     /locus_tag="JN749_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369420.1"
                     /translation="MRVKTMGASLLSGRIFQGTLNTEKGMWVGKREDVTDQAVRAVAE
                     HMMIKDQKYAYETKDGKWLIISHQLVDKLPEEFVDG"
     gene            complement(6611..6943)
                     /locus_tag="JN749_003091"
     CDS             complement(6611..6943)
                     /locus_tag="JN749_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7369421.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRTERKNKRTWYSKPRSEIGVTCSGRQKQRG
                     KSIPAYYD"
     gene            complement(7404..7808)
                     /locus_tag="JN749_003092"
     CDS             complement(7404..7808)
                     /locus_tag="JN749_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369422.1"
                     /translation="MIRIAALLSILLTTSANSECWIVTNLHGYGSMNGDRYEFTKDST
                     EDSVFHVTINGDKSSVYESVSGVYPEMKYTALSSNTMVGEYQSGGGITVETWSITTDK
                     KALYSKVMNIPGMQQLTSTKSFVGDVVGTCNQ"
     gene            complement(7805..8437)
                     /locus_tag="JN749_003093"
     CDS             complement(7805..8437)
                     /locus_tag="JN749_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HAY7369423.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            8541..8756
                     /locus_tag="JN749_003094"
     CDS             8541..8756
                     /locus_tag="JN749_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7369424.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            8876..9169
                     /locus_tag="JN749_003095"
     CDS             8876..9169
                     /locus_tag="JN749_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369425.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            9202..9348
                     /locus_tag="JN749_003096"
     CDS             9202..9348
                     /locus_tag="JN749_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2740 domain-containing protein"
                     /protein_id="HAY7369426.1"
                     /translation="MTKQLSPYQDKIHKHILRDRFLSSFKQPGRFRAELEKVKLMQKE
                     KGHE"
     gene            9341..10174
                     /locus_tag="JN749_003097"
     CDS             9341..10174
                     /locus_tag="JN749_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAY7369427.1"
                     /translation="MSNISNLAEAREARRLQQPHQSSGKGYALLHRKIMDVPFYKDAE
                     AAHLWVHLILKAKHTPEYVMTDAGEILVGRGKLLGGRNSLAFETGLKPDRVQYLLRKF
                     KKLGMIDWVSHGKFSVFSVEKYDDYQSNFVPADYQQITTDKEYNNIISNTDVLESTAA
                     DKKSDKKKPSVSCQDVVDAYHEILPEAPKIRALNDKRKNQIRTFWRKAGVITRQLDGH
                     GFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGFDFLLNDNTYLKVREGEHDD
                     R"
     gene            10164..11600
                     /locus_tag="JN749_003098"
     CDS             10164..11600
                     /locus_tag="JN749_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7369428.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPSETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11677..12117
                     /locus_tag="JN749_003099"
     CDS             11677..12117
                     /locus_tag="JN749_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7369429.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            12114..>12225
                     /locus_tag="JN749_003100"
     CDS             12114..>12225
                     /locus_tag="JN749_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7369430.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDV"
BASE COUNT         2969 a         3005 c         2721 g         3530 t
ORIGIN      
        1 ttttcagtcc acatgcgatt gaggcaagag ttgcgctact gatgccaaca tcgccagtcg
       61 attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccaa taaaaaaccg
      121 ccatcaggcg gcatggtgtt ctttcagttc ctcaattcga atattggtta cgtctgcatg
      181 tgctatctgc gcccatatca tccagtggtt atagcagtcg ttgatgtcct ctgcttcgat
      241 aaccctgtcg aatggctctc cattccattc acctgtgact cggaagtgca tttatcatct
      301 ccataaaaca aaacccgccg tagcgagttc agataaaaga aatccccgcg agtgcgagga
      361 ttgttagttg ctatctgctg cttccttggt cattaccata tccacccaat ttcaccagac
      421 atgattctcg caatcactat tatcaccaaa gtgataatca caactttaac tggcggcatc
      481 attcaccatc ctgctccggc ggttctagca gcggcatcca gtgggttacg tcatccaaga
      541 tatttcctga taaatatgtg aaagctctat attttttgta atcaattgga ctcattaccc
      601 agttccaata tgctgccact atttgacctt gactaaatgc cagtaacatt ttggtgtctt
      661 ccggcattcg ctcactacag cttatccaac catccggagt taccggatag ttgcgcattg
      721 cgacctttaa tgcctcatag aagcaacctt tcagattgtt gaactgacgc ccatttagcg
      781 ggccatgttc agtcagcatg ttgtgtaatt tccatgccgc gtcgtttact tcgttggatg
      841 acaggggagg caacttgtaa gtttggctta caggttcggc accatgaagc atggcgtcgc
      901 tccgctctat gccatccagc gcgattcgta gtgcctgaat tgtggtagag ctatcgtttg
      961 gggctattcc atatcgctcg aatacagcta aatggttgcg cataatctca ggcgtaagct
     1021 ctttgtaagc ataagcgaga ggctctgatg cattatccgg cacaaccgac gcaggcgcgg
     1081 cagcataaac aggaataacg tccgcttgct ctttattgct ttcatccgtt aaagcccaga
     1141 ataatttccc ggccggatgt ttgaaaatat aagcaactgg ctctgcttcc agcgatgcca
     1201 gtgcaattcg tgccagttct tccgcttctt ctgctggcag tacaacgttg ctaccaggtc
     1261 cgtatgtttc gcgccactgc ttgattgtca gtagtcgctc tttggtaata gtggtcatct
     1321 cactctcctt tgatgcgaat accagtagcg cggattgcat cgatgacttc agaaactttg
     1381 tatgccatta ccgtttggta atcctcgtga aaatctgttc gatgaagcat gctgctacgt
     1441 tccgggagca gtatttcccg ctcctccagt tctgctatgc gcttctctgc ggattccagc
     1501 ttctcgcgca tatcgtcaac gtactcgacc agagaaccgc cagcaggaat ttcgcactcc
     1561 tcgaccagtt ggaagtagat atcagctgcg gcccgtgtgt tgctatacct agcgtcgccc
     1621 atctcacctt cacgaagagc atcgcgttcg gcggtaagat tggctatttt gctgtctttg
     1681 ccttccagct caacgcgcag cttccctacc gtaagcgcaa tatcctcgtt ctcctggtcg
     1741 cggcgtttga tgtattgctg gttcctttcc cgttcatcca gtagtgcctg cgctacttca
     1801 gggttgaaag ctgcgatata acgagcgttg ttctctgcgt tgttctgtcc atcaaagccg
     1861 gtccatttga taacgtcttc acatcgttta tcaccaggtg tatgcaccgc atatgtacca
     1921 gtaccagacg aaataaatgc gacccattca ccctgtgttg ccagttgcgc cgctttacgc
     1981 agtgcctggt aattaatttc gctcactggt tgcctccttt gctgggcttt ctaacttttg
     2041 agtggttgta tcaaattcaa acaacttaac cacgtcatca aacaggacat aatcaccatc
     2101 agaatcttca gtcatgtcag cgccacaatc ctgaccgcat gagtcacaac cacccatatc
     2161 aagctcgtat cgcttcaggt ttgcgatatt tgataaattc agcgccagta cagccaggtc
     2221 ataaacctct tcggcagtga catcgctgct cagtcccatt tcatggcgat atatgatttt
     2281 ttctactcgt tgttttgtga ttgtcatttt tgctcgcctc cctgttcttc cagaaaaata
     2341 cgcatagtct caagcatctc ttcggtgtca tacggtgaca acttgtcacg caggatgtgt
     2401 tcaatgctgt taatgaactt gcggattgct ttgcgttcaa tttcagccag aaaagcatcg
     2461 gttgccgggg tttcaggttt aatggcttca agcacggcgc gaatgacatc ggtatcgttc
     2521 tccacccatg accattcgct tgtatcattc cagtcgtggt ctatgatagc tgtttcattg
     2581 aatgcctcga ctgccgtatc cgggattact tcatgattta aagctgcctt cagccccgca
     2641 ttctccgccg ccagcgccgc gcacttggcc tccgcttcag caaatttacg caccagatat
     2701 tcagcgtttg tttcgttaac ctttaaatca cttgggatgc atttaccttt cagaaatcca
     2761 tccatctcaa ttagtgtcat ttgtttcatt tcttcccatt ccgccacatt gcattcagat
     2821 atttgttttg atttactgac ggaaaagaat ttcttttaag caattcctct ctcgatggca
     2881 ttggctttac gcgttggcga ataatcattt ctgccggaag aatgccgaga ttgtatgcaa
     2941 gtcctctcat ggtaaattcc tcagtcatta ctgatagcgc catagcgtga gcggtaatta
     3001 cgcaggcgcg ggtcaatttc agggaagtgg gtatatgtgg ctttgcggaa tggtcggatt
     3061 gatgtctggt aaattcgctc gcgctcttct ttctctgcaa gccatatgca gtggcgaaat
     3121 tccttttcct ctttcgtttc ctgcggtagc gacattatcc ggtcgtagtt tttcctgaat
     3181 ttatccagca cctccgatac ggaattgccg gaacagcggc gcgggtcatc cgcaccatac
     3241 tgaggcgctg gcatgatttt ctcctgatta aattgcgtga atagcgtgac gagggaaggg
     3301 gagagttact ggtgcaaagg gtatatcgtc gtcaaaatcc atcggaggtt cgttgtgttg
     3361 tgctggtgat gattgctgct gtggcttctg tgattgcctg ctggctgctt gttgtttgct
     3421 gtcgccaatg ccgccaagca tttgcatcac gccattaatt ccgacatgaa cctcggttgt
     3481 gtaacggtct tgccctgact ggtctttcca ctttctggtt ctcagcattc cctcgaaata
     3541 aatctgatca ccttttttca catactgccc cacgacctca gccagtttcc cggatacagc
     3601 aacacgatgc cattcagtca attccttttg ctcgccagta tttttatctc gccattgttc
     3661 tgacgtggct attgtcaggt tagcgaacgc tgttcctgat ggtgagtatc gaacttccgg
     3721 gtcttgtcct acccgaccaa ggataatcac cttatttacg cctctgcttg ccatttatgc
     3781 cgcctgtttt agttcgttaa ctctgatgtt cattacctga acgcatttag cctgcgcttc
     3841 ctcgttgcca gccattaatt gccagtcacg ctgataacgc tcgatgagtt ttttcttgtc
     3901 agtttctgtt gacgcataat cgctgaagtc tttcaggatt tgttcgcagt caaccgatgg
     3961 agatttctgg ttggtatttt ctggtgatgg tttgttatct gatgctggga ttgcccatcc
     4021 cggcagcgat ggagggagcc agtaaaatcc tgttccatcc ttcagttttg ccctgtgcca
     4081 cccatgcttt ttatcgagag atgtttgtgc gaaaccttcc tcaaggttat acagataccg
     4141 accgattccc cactgaacgg cagcacgctt cattgcacct gaacgaccac ctttgacggc
     4201 ttctacctgc gtgttttcag cagcatccca tttggttacc cattcggaat caatcctgat
     4261 tgatatgccg cattcaacgc ctccgttgtt gggaatatcg cggtattcat tgcgccatcc
     4321 tgctttgccg caaacatcgt ccaggcgttt catgattgcc cggttcgtga cataagccag
     4381 caccatagcc cacactttgc catcgcgtgt tttaccgctt tgctgtattc gccattcgat
     4441 atcttcaggg ctgaatggct catcgaattt attcaaatcc ataaatcacc tcagaatgga
     4501 catggcccaa gaaaataacg ctgatttaat acttcgactc gggacaaatt aaggcatacc
     4561 cgcattcctt cgcggtcgcc attatggcga taccagagag ctttctgggt gtacatgcgt
     4621 ctctgtaact tgctctcctt cactgtggtt gcaagtgaca tgaatatctc cttcgtttcc
     4681 gattaattct ttcatctgac gaatgaattc ttcgtctgac cagttatctg taaaactcat
     4741 ttcctgcgat accacggaag gttgatagct gacttcatcg ctttatttgc ttcaagccac
     4801 attttggaat caccaataaa tctggctatt actgctttgt tctgtgcagc acgaagcatc
     4861 tggtgattga tggctatttc attgcgcata ataagacatc aactcttttc catccgtcac
     4921 gtaatttacg ggtgattcgt tcaagtaaag attcatttaa ttggaaggca cccatgcgag
     4981 cgcctcccgc gattgcgtaa atcatgggtg gttccttatg ttggttttat tagtaggtta
     5041 tttttgttgc gaatacttcg ccttttacga tggctgttat gatattttta gcaacatctt
     5101 ctgatgcgcc aaccttgata aggtcagcaa gtattttgtt atttacttct tttcggtgag
     5161 ctttatcctt tgctctacgc tcttcttcgt ccttgattct tttttcttct gctattctgg
     5221 cttgctcttt tgcttcagct tcgcgccgga ttcgttcagc ctcctcctgt gcttttcggc
     5281 gttctgcttc aattgccgcc tgcttttctc tttcagctcg ttctgctgcc tcttttgctt
     5341 cgcgctgtgc tcgttgctcg gcttcaatgc gttcacgctt tgcacgttcc gctgcggcct
     5401 tagcttctgc ttctcgcctt gctgctgctt caatttcggc ttttgccttt gcttcggctt
     5461 ctgctctggc tttctcttca gcttctcttt ttaagcgttc ttcatgctct cgcttttcct
     5521 gctccgcttt gagtcttgcc tcttctcttt ggcggtcaaa ttcgcgatcc atcaaaatcg
     5581 ctatttcatg gtcagactca atttgctttg cgagagcttc agctgctgcc ttagcttctt
     5641 cttcggcttt aatccgcgcc tgttcctcct cataatcagt aagaggctgg cgcgccttgg
     5701 ctttcagctc atcaagtcga tcatgcactg ttttgcggtt ggcatcaatt agctttggaa
     5761 tttccttcag ttcagcaaca aggtctttgc caagaccatc gagatatgtt ttcgtctgcg
     5821 caactttata cgccagagaa gcgatctcct ttctgccctt tgccgttgtg atatcaggca
     5881 caaaggacat aacttcacgt tcaacctttt gaaggatttc ttcaatctgg tcggcagact
     5941 gaaatacagt cattgcattt gctttttcaa taacaactaa atctgttact tcactcatat
     6001 ttcccccgtc aaaaaaattg ccctcacact ggagggcaaa gaagatttcc aataatcaga
     6061 acaagtcggc tcctgtttag ttacgagcga cattgctccg tgtattcact cgttggaatg
     6121 aatacacagt gcagtgttta ttctgttgct tattccaaag caaatttaac catcaacaaa
     6181 ctcttctggt aatttatcaa ccagttgatg acttattatc agccatttgc catccttcgt
     6241 ttcgtatgca tatttctggt cttttatcat catgtgttcg gctactgccc tcactgcctg
     6301 atcggtgaca tcttctctct ttcctaccca cattcctttt tcagtgttta atgttccttg
     6361 aaaaatacga ccacttaata ggcttgcgcc catagttttt actctcatgt atcagtcctc
     6421 aaataagtgg tttgctgcga aagtaaatac gcttaagtta cctgttattt atcccaccaa
     6481 gttccgtatc tatctatcca gttacaccaa tcatcgacac tctattttgt tgtgtcgcat
     6541 tttggcaact ggcaagaata tctaccttct ttgtaaagtc ggcgtttgac tttcttgagc
     6601 atggctcacc tcaatcgtaa taagctggaa ttgattttcc gcgttgcttc tggcggcctg
     6661 agcaagtcac acctatttca ctgcgtggct tgctgtacca tgtgcgctta ttcttgcgct
     6721 cagtacgctg caggttgctt tcaatctgtt cgtggtattc agccagcacc gtaaggtcta
     6781 tcggattcag tgcgctttct actcgtgatt tcggtttgcg attcagcgag agaatagggc
     6841 ggttaactgg ttttgcgctt accccaacta acaggggatt tgctgctttc cattgagcct
     6901 gtttctctgc gcgacgttcg cggcggcgtg tttgtgcatc catctggatt ctcctgtcag
     6961 ttagctttgg tggtgtggtg gctggtagtc tagctccagc ttgttgagtc tcattcggag
     7021 gggtataacc ggcaccccag cgatttttcc atgcgacaac gtgcgcgtta tggcggcctt
     7081 atcgcccgcg gctccccatc tcgtccacgc tattgctagc gttgggagcg cttcaccgct
     7141 caacagtagg taagcacttg ccagtgacta gctggcttca ccacacccca aagccttctg
     7201 ctttgaatgc tgcccttctt cagggcttat tttttaagag cctcaccttc atggtggtca
     7261 gtgcgtcctg ctgatggctt aaaattacaa gaaagattgt atgttgtaaa caagaaatat
     7321 tgtgaaaagg ggcgtgaaaa acaaactcca ttgtttttaa atgaaaaata gtttgttttt
     7381 ggttatcgag attgaggtgg ggattactgg ttgcaggttc cgactacatc accaacaaat
     7441 gatttggttg atgtaagttg ttgcatacct gggatattca ttactttgga gtaaagagct
     7501 tttttgtctg tagtgattga ccaagtttca acggttattc ctcctccaga ctggtattct
     7561 cctaccatag tgttcgatga caaagcagtg tatttcatct ctggatagac gccagaaact
     7621 gattcataaa ctgatgattt atcgccattt attgttacgt ggaaaacgga atcttccgtg
     7681 ctgtcttttg taaactcgta acgatcgcca ttcattgacc cgtacccgtg caggtttgtg
     7741 acaatccagc attcagaatt ggcgctggta gttaagagta ttgagagtag cgccgcaatc
     7801 ctgatcatac gaattttacc ctcgcttcca cgacaacacc gataatcttg cagttcccgt
     7861 tgataggagt cataggccat gaagggttca ggcctttcag gtacttctgc ccgccatcta
     7921 taaccagttt cttgaatgtt gcttcgttcg cgtcagtcag tttggctaca acaaggcttc
     7981 cattcactgg ctcgcgtcca gtatctacta acaccatatg accttcaggg atgctttgac
     8041 ctacaggtga ggtcatggaa tcaccttcaa ccttcagcca gaatccatcg cctaataagt
     8101 taacgtcact gtcataccat tcatcaatgt ccttgatatc gtagggttca caagcttcac
     8161 accacgaacc agctctaacc atgctaatca atggatattt ccctttgggt tcaacatgcc
     8221 caacaaatct aacattcgaa tcagaggtgc cattgagcag ccagtcaaca cttacgccaa
     8281 gagctgacgc aagttctggt aaaaagcgtg gtcgcttagt tttaccgttt tcgagctgct
     8341 ctatagactg ctgggtagtc cccacctttt gagcaagttc agcctggtta agtccaagct
     8401 gaattctttt gctttttacc ctggaagaaa tactcataag ccacctctgt tatttacctc
     8461 caatcttcac aagaaaaact gtattttaca aacaagatac attgtatgaa aatacaagaa
     8521 agtttgttga tggaggcgat atgcaaactc tttctgaacg cctcaagaag aggcgaattg
     8581 cgttaaaaat gacgcaaacc gaactggcaa ccaaagccgg tgttaaacag caatcaattc
     8641 aactgattga agctggagta accaagcgac cgcgcttctt gtttgagatt gctatggcgc
     8701 ttaactgtga tccggtttgg ttacagtacg gaactaaacg cggaaaagcc gcttaagaca
     8761 ttcccgctct tacacatccc agtcctgaaa aagggcatca aattaaacca cacctatggt
     8821 gtatgcattt atttgcatac cttcaatcaa ttgttatcta aggaaatact tacatatggt
     8881 tcgtgcaaac aaacgcaacg aggctctacg aatcgagagt gcgttgctta acaaaatcgc
     8941 aatgcttgga actgagaaga cagcggaagc tgtgggagtt gataagtcgc agatcagcag
     9001 gtggaagagg gactggattc caaagttctc aatgctgctt gctgttcttg aatggggtgt
     9061 cgttgacgac gacatggctc gattggcacg acaagttgct tcgattctca ccaataaaaa
     9121 acgcccggct gcaaccgagc gttctgaaca aatccagatg gaattctgag gtcattactg
     9181 gatcaatcca caggagtaat tatgacaaaa caactcagtc cttaccagga caaaattcac
     9241 aaacacatac tacgtgatcg cttcctgtcc agcttcaagc agcctggtcg attccgggct
     9301 gagttggaaa aagtgaagct gatgcagaag gagaaaggtc atgagtaaca tatctaatct
     9361 agccgaagcc agagaggcca gaaggctaca acaaccgcat caaagcagcg gtaaggggta
     9421 tgccttgctg caccgtaaaa ttatggatgt gccgttttac aaggacgcag aagcagcgca
     9481 tctgtgggtt cacttaatcc tcaaagcaaa gcatacgcct gagtatgtaa tgactgacgc
     9541 aggagaaatt ctggtaggca gagggaagct acttggcggt agaaactctc tggcgtttga
     9601 aacaggactc aaaccagatc gcgttcagta cctgcttaga aagttcaaaa aactcggcat
     9661 gattgactgg gtttcacacg gtaaattctc agttttctcg gtagagaaat atgacgatta
     9721 tcagtcaaat tttgtaccag cagattacca gcaaattacc acagataaag aatataataa
     9781 tattatctct aatactgacg tattagagag taccgcagca gacaaaaagt ctgacaagaa
     9841 aaaaccttcc gttagctgtc aggatgttgt cgatgcttac cacgaaatcc ttcctgaagc
     9901 gccaaaaatc cgcgcactga atgacaagcg taaaaaccag atccgaacgt tctggcgcaa
     9961 agccggagtg ataacccgcc agcttgacgg gcatgggttc acgatgcagg actggagaaa
    10021 ttatttgagc tacgtaggcg aaaattgccg atggatgttc gaagagcgcc caaaccatca
    10081 gcgcggaacc gtctggcaca aaaagggatt tgatttcctg cttaacgata atacctacct
    10141 gaaagttcgt gagggtgaac acgatgaccg ataattttta tgcgccgccc catagcatcg
    10201 aggcagagca ggcggtgatt ggtggattgc ttctggatga tgacagcagt gagcgcgtcc
    10261 agaaggttct ggcgatgctg aagcctgatt cattttacag ccgaccacac aaaatcattt
    10321 tcgaagaaat aaccagaatg caccgggagc aaaagccagt agatggcctg acgcttttcg
    10381 atgaactgga gcgtaaatcg ttaacggcgt ctgttggcgg ttttgcttat atcgctgaga
    10441 tcgcaaagaa cacgccaagc gccgcaaaca tcgttgccta tgcaatgcag gttcgcgaaa
    10501 ccgcaatgga acgctacgcc atcaaccgca tgactgaagc gacggaattg ctctattccc
    10561 gcaacggaat gactgcgacg cagaagtacg aagctattca ggcgattttc acgcaactga
    10621 cagaccatgc aaaaacagga tcgcgtcgcg gccttcgctc atttggtgag gtcatggaag
    10681 actgggttag cgaccttgag aagcgatttg acccatcagg cgaacaacgg ggaatgagca
    10741 cagggatccc atcgctggac aggatgctgt caccgaaagg tctggtgaaa ggctctctgt
    10801 ttgtcattgg cgctcgacct aagatgggga aaacgacgct atacagccag atggcaatca
    10861 actgcgcagt gcatgagaaa aagcccgctc ttatgttcag ccttgaaatg ccaggtgacc
    10921 agatactgga aaaactggta gggcagaagt ctggtgttaa cccgaatatt ttttaccttc
    10981 cggcgacaaa tgacgctgat gacggctatc agggtgatta cgatggtgac ttcaacaggg
    11041 cgatcgaaac agccaatcgc ttgagtgaaa tcgacctgct ttacatcgac gacacgccgg
    11101 gattatctct ggctcaaatc gtcagcgaaa gccgtcgaat caagcgagaa aaaggatgtg
    11161 ttggcatgat tctggtcgat tacctgacac taatgaccgc tgaaaaggcc gatcgcaacg
    11221 accttgctta cggcatgatc accaaaggac tgaagaacct tgccaaagag cttgattgcg
    11281 ttgttgtgct tctgacacag cttaaccgcg cactggaaag ccgaaccaat aaacgcccat
    11341 taccaagtga ctcacgagat acagggcaga ttgaacagga ttgcgattat tgggtgggga
    11401 tccatcgtga aggtgctttt gatgacagtg ttccacctag tgaaaccgaa ctaatccttc
    11461 gtctcaatcg tcatggcaat accggcacgg tgtattgcat tcaggcaaat ggcgctattt
    11521 atgacacaga ccaacagtct gctgaaatgc gccgacgtga acgcgaggaa ccgcagtcca
    11581 agaagaaagg aggattctga tgaccatcta catcactgag ctaataacag gtctgctggt
    11641 aatcgcaggc ctttttattt gggggagagg gaagtcatga aaaagctaac ctttgaaatt
    11701 cgatctccag cacatcagca aaacgctatt cacgcagtac agcaaatcct tccagaccca
    11761 accaaaccaa tcgtagtaac cattcaggaa cgcaaccgca gcttagacca gaatcgaaag
    11821 ctttgggctt gccttggtga cgtctctcgt caggttgaat ggcatggtcg ctggctggat
    11881 gcagaaagct ggaagtgtgt gtttaccgca gccttaaagc agcaggacgt tgttcctaac
    11941 cttgccggga atggctttgt ggtaataggc cagtcaacca gcaggatgcg agtaagcgaa
    12001 tttgcggagc tattagagct tatacaggca ttcggtacag aacgtggcgt taagtggtca
    12061 gacgaagcgc gactggctct ggagtggaaa gcaagatggg gagacagggc ggcatgaggc
    12121 gacagcgacg aagtttcacc gacatcatct gcgaaaactg caaatacctt ccaacgaaac
    12181 gctccagaaa taaacgcaag ccaatcccaa aagaatctga cgtaa
//
LOCUS       DADLTF010000118        12153 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.118, whole genome shotgun sequence.
ACCESSION   DADLTF010000118 DADLTF010000000
VERSION     DADLTF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.118"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            127..996
                     /locus_tag="JN749_003101"
     CDS             127..996
                     /locus_tag="JN749_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369431.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALPLKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYARTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1199..1552)
                     /locus_tag="JN749_003102"
     CDS             complement(1199..1552)
                     /locus_tag="JN749_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HAY7369432.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1690..3336)
                     /gene="groL"
                     /locus_tag="JN749_003103"
     CDS             complement(1690..3336)
                     /gene="groL"
                     /locus_tag="JN749_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAY7369433.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3380..3673)
                     /locus_tag="JN749_003104"
     CDS             complement(3380..3673)
                     /locus_tag="JN749_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAY7369434.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKSLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3949..5205
                     /gene="yjeH"
                     /locus_tag="JN749_003105"
     CDS             3949..5205
                     /gene="yjeH"
                     /locus_tag="JN749_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HAY7369435.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="JN749_003106"
     CDS             complement(5221..5697)
                     /gene="fxsA"
                     /locus_tag="JN749_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HAY7369436.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6034..7470
                     /gene="aspA"
                     /locus_tag="JN749_003107"
     CDS             6034..7470
                     /gene="aspA"
                     /locus_tag="JN749_003107"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HAY7369437.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7588..8889
                     /locus_tag="JN749_003108"
     CDS             7588..8889
                     /locus_tag="JN749_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7369438.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9005..9343
                     /gene="cutA"
                     /locus_tag="JN749_003109"
     CDS             9005..9343
                     /gene="cutA"
                     /locus_tag="JN749_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HAY7369439.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9319..11016
                     /locus_tag="JN749_003110"
     CDS             9319..11016
                     /locus_tag="JN749_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HAY7369440.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11053..11628
                     /locus_tag="JN749_003111"
     CDS             11053..11628
                     /locus_tag="JN749_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7369441.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11735..11810
                     /locus_tag="JN749_003112"
     tRNA            11735..11810
                     /locus_tag="JN749_003112"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11768..11770,aa:Phe,seq:gaa)
BASE COUNT         2926 a         3137 c         2986 g         3104 t
ORIGIN      
        1 tttctgttta tatagagtga gcacggctat ttatgttatt ttaacaacta ttactaccaa
       61 gcccatttat ctggcttata aattaaaatt aattatttaa ttgagaaatg gttagggaga
      121 acctacatgg ccataagcat caagggtgtt aataccggtg ttattcgcaa gagtaataat
      181 ttcattgcac tgccgctgaa aattaaagaa cctcgcaata aagaatcgtt gttctttatg
      241 tctgtcatgg aactgcgcga cctgctgatt gcgctggaaa gtcgtctgca tcaaaagcat
      301 aaactggatg ctgctgcacg ccttcagtat gagcaagctc gcgataaagt cattaagaaa
      361 atggcagaaa acatcccaga aattctggtt gatgagctta aaaatgccga tattaatcgt
      421 cgggttaaca cgctggaact taccgataat caaggcgaaa atcttacctt cgtattaacg
      481 ctacatgacg gtagcaaatg tgagttagtc gtcaatgagt tgcagattga aatgctggcg
      541 cgagcaatca ttcatgcaat taataatgcg gaaatgcgtg aactggcatt acgtattacc
      601 tcactgttag atttcctgcc gttatacgac gttgactgcc aggacaatgg caatctggaa
      661 tatgacacct attcccaacc ggaatggaaa cataacttgt tcgaccatta tctggcggtg
      721 ctttaccgct ttaaagatga aagtggcaaa gaacaattta gcggcgcagt ggtgaaaacg
      781 cgtgaagcaa cgccgggtaa agaaatagag gcgatcactc gccgaatgct tgatttcagc
      841 ccacgactga aaaaactcgc gggtgtgcca tgtcaggtct atgctcgcac cgtagcggcg
      901 aataacgccc aaccgctaac ccaggatcaa tgcttacgcg cgctgcatca cttacgtgta
      961 caatccacca gcaaaactgc accacaggcg aaataatctc atctttgcta atgcgaggct
     1021 ggagccatcc agcctgaaat gactgataac gtgatcgttg gtcatgccgg atgcggcgta
     1081 aacgccttat ccggcctaca aattctcaag cattcaatca gttgcaatgc ctgctgtagg
     1141 cctgataagc gaagcgcatc aggcaataat acgtttgtca cccagcctct ggatcacatc
     1201 agttcgggca cttataaacc tgaccgataa tctggctatc cgtcgggaca aaactggaca
     1261 acattccctg cgacgggctg ctgatgccat aaatcacgtt accgcccatt gcagccgcct
     1321 ggttgcgcag atcgtttgct gcgccgcgca tagaaccgcc ctcttctccg tgttgcccgg
     1381 aaagccagtt gctttgctta cctgtcgcag taccaatcag ctggcactct gcgcctggct
     1441 gctcgtccac aatgcgtaca ctctgaccgg cagcactcaa ttcgttgctg gagctacaac
     1501 ccgccatcag tagcgcggca ccgacaatcc ctgctaagta ttttacgtgc atgttattcc
     1561 ccataatcaa tgagctggac gccactcgcg tcgtccgtgt ctgaatctta tactagaaag
     1621 atgaccaaaa gaaaaacccc cagacatttc tgcccggggg tttgtttatt tctgcgaagt
     1681 gcagggcaat tacatcatgc cgcccatgcc acccatgccg cccataccgc cagcagcgcc
     1741 taagtcagct gcatcgtttt tcggcaggtc ggtaaccatg cattcggtgg tgatcatcag
     1801 gccagccaca gaagctgcgt actgcagagc agaacgggtt actttggttg ggtccaggat
     1861 acccatgtcg atcatgttgc cgtattcttc ggttgctgcg ttgtaaccgt agttgccgtc
     1921 gccgccttta acggtgttag caacaacaga cggttcttcg ccgcagttca ggacgatctg
     1981 acgcagcgga gcttccattg cacgcagtgc aactttgata cccacgttct ggtcttcgtt
     2041 ctgaccacgc aggtcagcca gtttagacgc tacgcggatc agcgcaacac caccaccagc
     2101 aaccacgcct tcttctaccg cagcacgggt cgcgtgcagg gcatcttcaa cgcgtgcttt
     2161 tttctctttc atttcaactt cggtagcagc acctactttg ataactgcaa cgccgcctgc
     2221 cagtttcgct acgcgctcct gcagtttttc acggtcgtag tcagaagttg cttcttcaat
     2281 ctgctgacgg atctgagcaa cacggccctg gattgcagct tcttcaccca cgccatcgat
     2341 gatagtggtg gtgtctttgt tgatcacaac acgtttagcc tgacccaggt cttccagggt
     2401 tgctttttcc agctccatac cgatctcttc agagattacg gtaccgccag tcagggttgc
     2461 gatatcctgc agcatagctt tacgacgatc gccgaagccc ggagctttaa ccgcagcaac
     2521 tttcacgatg ccacgcatgg tgttaacaac cagagttgct agcgcttcgc cttcaacatc
     2581 ttcagcgatg atcagcagcg gtttgcctgc tttggcaaca gcttccagaa ccggcagcat
     2641 ttcgcggatg ttggagattt tcttgtcagc cagcaggatg aacgggcttt ccagttctac
     2701 tgcgccagtt tccggcttgt tgatgaagta aggagacagg tagccacggt cgaactgcat
     2761 accttcaacc acgtccagtt cgtcctgcag accggtaccg tcttcaacgg tgataacgcc
     2821 ttctttaccg actttgtcca tcgcttcagc gatcagttta cctacggttt cgtcggagtt
     2881 agcggagatg gtaccaacct gagcaatcgc tttagaatca gagcacggta cggacagcgc
     2941 tttcagttct tcaactgcag cggtaaccgc tttgtcgata ccacgtttca ggtccatcgg
     3001 gttcatgccc gcagcaacag ctttcagacc ttcagtgatg atagcctgag ccagtacggt
     3061 tgcagtggtg gtaccgtcgc ctgcagcgtc gttcgctttg gaggcaactt ctttcaccat
     3121 ctgcgcaccc atattttcga acttgtcttc cagttcgatt tcacgagcaa cggaaacacc
     3181 atctttggtg atggtcggtg caccgaaaga tttatccaga actacgttac ggccttttgg
     3241 accgagggta actttcactg catctgccag tacgtttacg ccgcgcagca ttttcacacg
     3301 agcgtcgtta ccgaatttta cgtctttagc tgccattatc tttattcctt aaattcgtat
     3361 gttcagtgtc gtgcgcggat tacgcttcaa caattgccag aatgtcgctt tcggacatga
     3421 tcaacacttc ttcattgtcg atcttctcgg atttcacacc gtagccatcg ttgaaaataa
     3481 cgatgtcgcc aactttcacg tccagcgact tcacttcgcc attttcaagg atacggccat
     3541 tgccgacagc cagcacttcg ccgcgggtgg atttagccgc tgcagagccg gtcagaacga
     3601 tgccgccagc agatttagtt tcaacttctt tacgcttgac gatcacgcga tcatgcaatg
     3661 gacgaatatt cattgataac tctcctttga gaaagtccgt atctgttatg ggtgacgccg
     3721 gagcttacgt ggtttcccgg ctggtgacca gagaaatggg gatgaggctt cgccccttca
     3781 agggggaaaa agaaaaaaaa ttctgatcag cacaaaatcg ggtgaaaacc ctgattcacc
     3841 tcacatttca tcgcaatttc ttcatcgccg tcataagcga atctgattgt gctaccatcg
     3901 aaaatctacg cagttgccca aaatttgggc gcaatcggat atcaacccat gagtggactc
     3961 aaacaagaac tggggctggc ccagggcatt ggcctgctat cgacgtcatt attaggcact
     4021 ggcgtgtttg ccgttcctgc gttagctgca ctggtagcag gcaataacag cctgtgggcg
     4081 tggcccgttt tgattatctt agtgttcccg attgcgattg tgtttgcgat tctgggtcgc
     4141 cactatccca gcgcaggcgg cgtcgcgcac ttcgtcggta tggcgtttgg ttcgcggctt
     4201 gagcgagtca ccggctggct gtttttatcg gtcattcccg tgggtttgcc tgccgcacta
     4261 caaattgccg ccggattcgg ccaggcaatg tttggctggc atagctggca actgttgttg
     4321 gcagaactcg gtacgctggc gttggtgtgg tatatcggta ctctcggtgc cagttccagt
     4381 gctaatctac aaaccgttat tgccggactt atcgtcgcac tgattgtcgc tatctggtgg
     4441 gcgggcgata tcaaacctgc gagtatcccc ttccccgcgc caggaaatat cgaacttacc
     4501 gggttatttg ctgcgttatc agtgatgttc tggtgttttg tcggtctgga agcatttgcc
     4561 catcttgcct cggaatttaa aaatccagag cgtgattttc ctcgtgcttt gatgattggt
     4621 ctgctgctgg caggattagt ctactggggc tgtacggtag tcgtcttaca cttcgacgcc
     4681 tatggtgaac aaatggcggc ggcagcatcg cttccaaaaa ttgtagtgca gttgttcggt
     4741 gtaggagcgt tatggattgc ctgcgtaatt ggctatctgg cctgctttgc cagtcttaac
     4801 atttatatac agagcttcgc ccgcctggtc tggtcgcagg cgcaacataa tcctgaccac
     4861 tacctggcac gcctctcttc tcgccatatc ccgaataatg ccctcaatgc ggtgcttggc
     4921 tgctgcgtgg tgagcacgtt ggtaattcat gccttagaga tcaatctgga ggctcttatt
     4981 atttatgcca atggcatctt tattatgatt tatctgttat gcatgctggc aggctgtaaa
     5041 ttattgcaag gtcgttatcg actactggcg gtggttggcg ggctgttatg cgttctgtta
     5101 ctggcaatgg tcggctggaa aagtctctat gcgctgatca tgctggtggg gttatggctg
     5161 tttctgccaa aacgaaaaac gccggaaaat ggcataacca cataatccgg cgtttcgaca
     5221 ttaatcctgg cgatcgtctt tatgatcaag gcggtcgcgg tcatcatcct ttcgctgata
     5281 ctcaccatca aaagtattac cgccaccggt cccggcgcta aaaccgccgc ctggcatgcg
     5341 agaaaagcgc aaatgtggca tcaacttcac tgtcagatgc ttttgcaccg gcggcaataa
     5401 aagcagaaga ccgaggaagt cggtgaaaaa gcccggtaat aaaagcagca aaccggcaat
     5461 aatcagcgaa acacttttaa tcatctccgc cgcagggttt tcacccgccg ccattttttg
     5521 ctgcatcagc acgaaattct taaagccctg gttacgtacc agtgacatac cgataactga
     5581 cgtgaatata accagcacga gggtcagcaa tacccccaat acatgggcaa cctgaataaa
     5641 gattgaaatc tcaatataga cataaaggaa aatggcaata aaaggtaacc agcgcaaagg
     5701 tttctcctgt aatagcagcc ggttaaaccc ggctacctga atgggttgcg aatcgcgttc
     5761 agcttatatt gtggtcatta gcaaaatttc aagatgtttg cgcaactatt tttggtagta
     5821 atcccaaagc ggtgatctat ttcacaaatt aataattaag gggtaaaaac cgacacttaa
     5881 agtgatccag attacggtag aaatcctcaa gcagcatatg atctcgggta ttcggtcgat
     5941 gcaggggata atcgtcggtc gaaaaacatt cgaaaccaca tatattctgt gtgtttaaag
     6001 caaatcattg gcagcttgaa aaagaaggtt cacatgtcaa acaacattcg tatcgaagaa
     6061 gatctgttgg gtaccaggga agttccagct gatgcctact atggtgttca cactctgaga
     6121 gcgattgaaa acttctatat cagcaacaac aaaatcagtg atattcctga atttgttcgc
     6181 ggtatggtaa tggttaaaaa agccgcagct atggcaaaca aagagctgca aaccattcct
     6241 aaaagtgtag cgaatgccat cattgccgca tgtgatgaag tcctgaacaa cggaaaatgc
     6301 atggatcagt tcccggtaga cgtctaccag ggcggcgcag gtacttccgt aaacatgaac
     6361 accaacgaag tgctggccaa tatcggtctg gaactgatgg gtcaccagaa aggtgaatat
     6421 cagtacctga acccgaacga ccatgttaac aaatgtcagt ccactaacga cgcctacccg
     6481 accggtttcc gtatcgcagt ttactcttcc ctgattaagc tggtagatgc gattaaccaa
     6541 ctgcgtgaag gctttgaacg taaggctgtc gaattccagg acatcctgaa aatgggtcgt
     6601 acccagctgc aggacgcagt accgatgacc ctcggtcagg aattccgcgc tttcagcatc
     6661 ctgctgaaag aagaagtgaa aaacatccaa cgtaccgctg aactgctgct ggaagttaac
     6721 cttggcgcaa cagcaatcgg tactggtctg aacacgccga aagagtactc tccgctggca
     6781 gtgaaaaaac tggctgaagt cactggcttc ccatgcgtac cggctgaaga cctgatcgaa
     6841 gcgacctctg attgcggcgc ttatgttatg gttcacggcg cgctgaaacg cctggctgtg
     6901 aagatgtcca aaatctgtaa cgacctgcgc ttgctctctt ctggtccacg tgccggcctg
     6961 aacgagatca acctgccgga actgcaggcg ggctcttcca tcatgccagc taaagtaaac
     7021 ccggttgttc cggaagtggt taaccaggta tgcttcaaag tcatcggtaa cgacaccact
     7081 gttaccatgg cagcagaagc aggtcagctg cagttgaacg ttatggaacc ggtcattggc
     7141 caggccatgt tcgaatccgt tcacattctg accaacgctt gctacaacct gctggaaaaa
     7201 tgcattaacg gcatcactgc taacaaagaa gtgtgcgaag gttacgttta caactctatc
     7261 ggtatcgtta cttacctgaa cccgttcatc ggtcaccaca acggtgacat cgtgggtaaa
     7321 atctgtgccg aaaccggtaa gagtgtacgt gaagtcgttc tggaacgcgg tctgttgact
     7381 gaagcggaac ttgacgatat tttctccgta cagaatctga tgcacccggc ttacaaagca
     7441 aaacgctata ctgatgaaag cgaacagtaa tcgtacaggg tagtacaaat aaaaaaggca
     7501 cgtcagatga cgtgcctttt ttcttgtgag cagtaactta aaaataacaa tctaatatca
     7561 acttgttaaa aaacaaggaa ggctaatatg ctagttgtag aactcatcat agttttgctg
     7621 gcgattttct tgggcgccag attgggggga ataggtattg gttttgcagg cggattgggg
     7681 gtgctggttc ttgccgctat tggcgttaaa cccggtaaca tcccgttcga tgtcatttcc
     7741 attatcatgg cggttatcgc cgctatttct gccatgcagg ttgctggcgg tctggactat
     7801 ctggttcatc agacagaaaa gctgctgcgc cgtaacccga aatacatcac gatcctcgca
     7861 ccgatcgtga cctatttcct gactatcttt gctggtactg gcaacatctc tctggcgaca
     7921 ctgccagtta tcgctgaagt tgcgaaggaa caaggcgtta aaccttgccg tccgctctct
     7981 actgcagtgg tatccgcgca gattgcgatc accgcatcgc caatctcagc ggcagtggtt
     8041 tacatgtctt ccgtgatgga aggtcatggc atcagctacc tccatctgct ctccgtggtc
     8101 atcccgtcca ccctgctggc ggttctggtg atgtccttcc tggtcactat gctgttcaac
     8161 tccaaactct ctgacgatcc gatttatcgc aagcgtctgg aagagggctt ggttgaactg
     8221 cgcggtgaaa agcagattga aatcaaatcc ggtgcaaaaa cgtccgtctg gctgttcctg
     8281 ctgggcgtag ttggcgtggt tatctatgca atcatcaaca gcccaagcat gggtctggtt
     8341 gaaaaaccgc tgatgaacac caccaacgca atcctgatca tcatgctcag cgttgcaact
     8401 ctgaccaccg ttatctgtaa agtcgatacc gacaacatcc tcaactccag caccttcaaa
     8461 gcaggtatga gcgcctgtat ttgtatcctg ggtgttgcgt ggctgggcga tactttcgtt
     8521 tccaacaaca tcgactggat caaagatacc gctggtgaag tgattcaggg tcatccgtgg
     8581 ctgctggccg tcatcttctt ctttgcttct gctctgctgt actctcaggc tgcaaccgca
     8641 aaagcactga tgccgatggc tctggcactg aacgtttcac cgctgaccgc tgttgcttct
     8701 ttcgctgcgg tgtctggtct gttcattctg ccgacctacc cgacgctggt tgctgcggta
     8761 cagatggatg acacgggtac tacccgtatc ggtaaattcg tcttcaacca tccgttcttc
     8821 atcccgggta ctctgggtgt tgccctggcc gtttgcttcg gcttcgtgct gggtagcttc
     8881 atgctgtaat gatcaatcgc ggggcgttca cgccccgctt tctttcccgc cgactaacat
     8941 ccttcccccg tccgttgtat agtgacctct ctcttgcggt tccatctgtt cttgcgaggt
     9001 gtttatgctt gatgaaaaaa gttcgaatac cgcgtctgtc gtggtgctat gtaccgcacc
     9061 ggatgaagcg acagcccagg atttagccgc caaagtgctg gcggaaaaac tggcggcctg
     9121 cgcgaccttg atccccggcg ctacctctct ctattactgg gaaggtaagc tggagcaaga
     9181 atacgaagtg cagatgattt taaaaactac cgtatctcac cagcaggcac tgctggaatg
     9241 cctgaagtct catcatccat atcaaacccc ggaacttctg gttttacctg ttacacacgg
     9301 agacacagat tacctctcat ggctcaacgc atctttacgc tgattctgct actttgcagc
     9361 acttccgttt ttgccggatt attcgacgcg ccgggacgtt cacaatttgt ccccgcggat
     9421 caagcctttg cttttgattt tcagcaaaac caacatgacc ttaatctgac ctggcagatc
     9481 aaagacggtt actacctcta ccgtaaacag atccgcatta cgccggaaca cgcgaaaatt
     9541 gccgacgtgc agctgccgca aggcgtctgg catgaagatg agttttacgg caaaagcgag
     9601 atttaccgcg atcggctgac gcttcccgta accatcaacc aggcgagtgc gggagcgacg
     9661 ttaactgtca cctaccaggg ctgtgctgat gccggtttct gttatccgcc agaaaccaaa
     9721 accgttccgt taagcgaagt ggtcgccaac aacgcagcgt cacagcctgt gtctgtttcg
     9781 cagcaagagc agcacaccgc gcaattgccc ttttccgcgc tctgggcgtt gttgatcggt
     9841 attggtatcg cctttacgcc atgcgtgctg ccaatgtacc cactgatttc tggcatcgtg
     9901 ctgggtggta aacagcggct ctccactgcc agagcattgt tgctgacctt tatttatgtg
     9961 caggggatgg cactgactta cacggcgctg ggtctggtag ttgccgccgc agggttacag
    10021 ttccaggcgg cgctacagca cccatacgtg ctcattggcc tcgccatcgt ctttaccttg
    10081 ctggcgatgt caatgtttgg cttgtttact ctacaactcc cctcttcgct gcaaacacgt
    10141 ctcacgttga tgagcaatcg ccaacagggc ggctcacctg gcggtgtgtt tgttatgggg
    10201 gcgattgccg gactgatctg ttcaccatgc accaccgcac cgcttagcgc gattctgctg
    10261 tatatcgccc aaagcgggaa catgtggctg ggcggcggca cgctttatct ctatgcgttg
    10321 ggcatgggcc tgccgctgat gctaattacc gtctttggta accgcttgct gccgaaaagc
    10381 ggcccgtgga tggaacaagt caaaaccgcg tttggttttg tgatcctcgc actgccggtc
    10441 ttcctgctgg agcgagtgat tggtgatgta tggggattac gcttgtggtc ggcgctgggt
    10501 gtcgcattct ttggctgggc ctttatcacc agcctacagg ctaaacgcgg ctggatgcgc
    10561 gtggtgcaaa taatcctgct ggcagcggca ttggttagcg tgcgcccact tcaggattgg
    10621 gcatttggtg cgacgcatac cgcgcaaact cagacgcatc tcaactttac acaaatcaaa
    10681 acgatagatg agttaaatca ggcgctcgtt gaagccaaag gcaaaccggt gatgttagat
    10741 ctttatgccg actggtgcgt cgcctgtaaa gagtttgaga aatacacctt cagcgacccg
    10801 caggtgcaaa aagcgttagc agacacggtc ttacttcagg ccaacgtcac ggccaacgac
    10861 gcacaagatg tggcgctgtt aaagcatctt aatgtccttg gcctaccgac aattctcttt
    10921 tttgacggac aaggccagga gcatccacaa gcacgcgtca cgggctttat ggatgctgaa
    10981 accttcagcg cacatttgcg cgatcgccaa ccgtgaacga cacttgcagt gggaaagacg
    11041 gaggaaaata gcgtgcaacg tgaagatgta ctgggagaag ccctgaaatt attagaatta
    11101 caagggattg ccaacaccac gctggagatg gttgctgaac gtgtggatta tccactggac
    11161 gagctacgcc gcttctggcc agacaaagag gcaatcctct acgatgcgct gcgttatctt
    11221 agccaacaga tagatgtctg gcgtcgtcag ctgatgctgg acgaaacgca aaccgccgaa
    11281 caaaagctgc tggcacgtta tcaggcgtta tcggagtgcg ttaaaaacaa ccgctatccg
    11341 ggctgtctat ttatcgctgc ctgtacgttt tatcccgatc ctggccaccc tattcatcaa
    11401 ctggccgatc agcaaaaaag cgcggcctac gatttcaccc acgaactgtt aaccacgctg
    11461 gaagttgacg atccggcgat ggtagcaaag cagatggaac tggtgctgga aggctgttta
    11521 agccgaatgc tggtgaatcg tagccaggcg gatgtcgaca ctgcacatcg gctggcggaa
    11581 gatatcctgc gcttcgcccg ctgccgccag ggtggtgcat tgacctgaca gaaacacaga
    11641 aaagaagcga tttgccgcaa tcttaagcag ttgaatcgct tttactgaaa ttaggttgac
    11701 gagatgtgca gattacggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca
    11761 ggggattgaa aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc
    11821 aacggacctc cacggaggtc cgtttttcgt ttcaggacgc cacgatttaa gcgttctgcc
    11881 tccaaatcaa ttctaccgaa ctcaaccaga ttctccccac atcaccagca atttgcgagc
    11941 atatcccaat ttgggaaaat ttgttcctga gctatagcac taactgacgt gaaatgtcgt
    12001 gcggccccgt gatgctgttg aacgtcaaat gacgtcatca ggagcgtaac gtatccataa
    12061 agcacaacac cggtctgaac gccgactgat gagattttct gaatgagaac aaagagaaat
    12121 gtgtcagtcc gcttgctcat gcaaagacta aca
//
LOCUS       DADLTF010000119        12128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.119, whole genome shotgun sequence.
ACCESSION   DADLTF010000119 DADLTF010000000
VERSION     DADLTF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.119"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="JN749_003113"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="JN749_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HAY7369442.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTVLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="JN749_003114"
     CDS             complement(2005..2928)
                     /locus_tag="JN749_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7369443.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGHAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINTIRKSMPF"
     gene            3145..4488
                     /locus_tag="JN749_003115"
                     /pseudo
     CDS             3145..4488
                     /locus_tag="JN749_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            4713..6368
                     /locus_tag="JN749_003116"
     CDS             4713..6368
                     /locus_tag="JN749_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HAY7369444.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTLQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="JN749_003117"
     CDS             6508..6732
                     /locus_tag="JN749_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HAY7369445.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="JN749_003118"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="JN749_003118"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HAY7369446.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="JN749_003119"
     CDS             complement(7326..8306)
                     /locus_tag="JN749_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HAY7369447.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="JN749_003120"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="JN749_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HAY7369448.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="JN749_003121"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="JN749_003121"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HAY7369449.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10983
                     /locus_tag="JN749_003122"
     CDS             10315..10983
                     /locus_tag="JN749_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HAY7369450.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(11001..11507)
                     /gene="tnpA"
                     /locus_tag="JN749_003123"
                     /pseudo
     CDS             complement(11001..11507)
                     /gene="tnpA"
                     /locus_tag="JN749_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11515..>12128
                     /locus_tag="JN749_003124"
     CDS             11515..>12128
                     /locus_tag="JN749_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7369451.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSF"
BASE COUNT         3135 a         2836 c         3044 g         3113 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg tgttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggccgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatacag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag tgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agcagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagact
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgt gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgtaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg ctgttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcactag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaagaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgctgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact cgcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cccgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga ggaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgcgcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatattct ggttttttaa acaattactc tgatttaaaa
    10441 gaaacaacct cagcaacagg taaacctgtt ttacgttggg tagacccgag ttttgatcaa
    10501 agcaaatatg acagcatcgt ctggaaccca atcacttatt atccggtacc aaaaccttct
    10561 acccaggtag ggcagaaagt tctggataaa attttgaact ataccaacac cgaaatgaaa
    10621 gaagcgatag cgcagcgtaa accagtggtt accaccgctg ggccgcgtag tctgattttc
    10681 cgtggggcca ttaccggtgt agataccagc aaagaagggc tgcaattcta tgaagtggtt
    10741 cctgttgcat tagtggttgc ggggacgcaa atggctacag gccaccgtac catggatact
    10801 cgcctctatt ttgaaggtga gctgattgat gcagcgacta ataaaccggt tatcaaagtc
    10861 gttcgtcagg gcgaaggtaa agacctgaat aacgaaagta cgccaatggc tttcgaaaat
    10921 attaaacaag ttattgatga catggcgacc gatgccacca tgtttgacgt taacaaaaag
    10981 tagttccaga cgcgccatcg ttagatggcg ctttttatcc ggtgcgccgt aaaaccccat
    11041 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    11101 atgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa atagccagga
    11161 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    11221 aacctacccg atacgccttt gaggctgttt accagactgg atatcgccag tttgggaggt
    11281 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagcttca
    11341 aaataagcac atacatttga aaagtaagtg cgtagttttt ctgtcgcgtc atggtcaaaa
    11401 atctggcgtc ggtatctggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc
    11461 cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa
    11521 cgattacagg catttaaatt ccagttaaga ccaggtggtc aacaggagtg tgaaatgagg
    11581 cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcacgtca gaatgagaat
    11641 catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg
    11701 aaaaatgcca ctgaaacgca atggcttaaa gattctccct cacagccatt gcaacagtca
    11761 ctgaaagacc ttgagcgggc ttacaaaaac ttcttccgga agcgggctgc ttttccccga
    11821 ttcaaaaagc ggggacagaa tgatgcattc cgctacccgc agggcgttaa gctcgatcag
    11881 gaaaacagcc gtatttttct gccgaaactg ggctggatgc gctaccggaa tagccgtcag
    11941 gtcacgggtg ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt
    12001 attcagacag aaagtgaagt atccactccg gctcaccctt cagcatcaat ggtcgggctg
    12061 gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac
    12121 agttttca
//
LOCUS       DADLTF010000120        12053 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.120, whole genome shotgun sequence.
ACCESSION   DADLTF010000120 DADLTF010000000
VERSION     DADLTF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.120"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..136)
                     /locus_tag="JN749_003125"
     CDS             complement(<1..136)
                     /locus_tag="JN749_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAY7369452.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     A"
     gene            349..1812
                     /gene="bepA"
                     /locus_tag="JN749_003126"
     CDS             349..1812
                     /gene="bepA"
                     /locus_tag="JN749_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HAY7369453.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSNLLDEWAKGNVLQQRAAQYGRALQAMKANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            1833..2192
                     /gene="arsC"
                     /locus_tag="JN749_003127"
     CDS             1833..2192
                     /gene="arsC"
                     /locus_tag="JN749_003127"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HAY7369454.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2325..3071)
                     /gene="hda"
                     /locus_tag="JN749_003128"
     CDS             complement(2325..3071)
                     /gene="hda"
                     /locus_tag="JN749_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HAY7369455.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(3121..4410)
                     /gene="uraA"
                     /locus_tag="JN749_003129"
     CDS             complement(3121..4410)
                     /gene="uraA"
                     /locus_tag="JN749_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAY7369456.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(4496..5122)
                     /gene="upp"
                     /locus_tag="JN749_003130"
     CDS             complement(4496..5122)
                     /gene="upp"
                     /locus_tag="JN749_003130"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAY7369457.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            5447..6484
                     /gene="purM"
                     /locus_tag="JN749_003131"
     CDS             5447..6484
                     /gene="purM"
                     /locus_tag="JN749_003131"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAY7369458.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            6484..7122
                     /gene="purN"
                     /locus_tag="JN749_003132"
     CDS             6484..7122
                     /gene="purN"
                     /locus_tag="JN749_003132"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAY7369459.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            7294..9360
                     /gene="ppk1"
                     /locus_tag="JN749_003133"
     CDS             7294..9360
                     /gene="ppk1"
                     /locus_tag="JN749_003133"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HAY7369460.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            9365..10906
                     /gene="ppx"
                     /locus_tag="JN749_003134"
     CDS             9365..10906
                     /gene="ppx"
                     /locus_tag="JN749_003134"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HAY7369461.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPSTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(10945..>12053)
                     /gene="pdeF"
                     /locus_tag="JN749_003135"
     CDS             complement(10945..>12053)
                     /gene="pdeF"
                     /locus_tag="JN749_003135"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7369462.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         3099 a         3083 c         3041 g         2830 t
ORIGIN      
        1 tagccaccag caacggagca agcaggccac taaagaaaaa gataatgcca aatccggcaa
       61 ctaaaataac cagcaaggca atcgcttccg ggtcgctaaa acggcggcga taccattgca
      121 tcaacatttc gagcataaag accttccctg aacctcaaga gcgggattgc gatccgcaat
      181 tgtatcgaaa tgtcacaaaa aagacttcgc tttttatgac ggattcagga aactgaaaag
      241 tcatttgagt gggctaatct tcgccgttac actcaaaggc ggcgaggtgg gaacgatatt
      301 tcacagtatc ggtcaaatga ctatcttcag aaatacagga tagaggttat gttcaggcag
      361 ttgaaaaaaa acctggttgc aaccctcatt gctgctatga ccattggtca ggtagccccg
      421 gcgtttgccg acagcgcaga caccttgccg gatatgggaa cctccgcagg aagcacgctt
      481 tccattggtc aggaaatgca gatgggcgac tattatgtcc gccagctacg cggcagcgcg
      541 ccgttaatta atgacccgct gttaacgcaa tatattaatt cgctggggat gcgtctggtt
      601 tcgcatgcca attcggttaa gacaccgttt catttttttc tgatcaacaa cgacgaaatt
      661 aacgcctttg ctttctttgg cggcaacgtg gtgctgcact ctgccctgtt ccgttattcc
      721 gataacgaaa gtcaactggc ttcagttatg gcgcacgaaa tctcccacgt cacccaacgt
      781 cacctggcgc gagcgatgga agatcaacaa cgcagcgcgc cgctgacctg ggtcggcgcg
      841 ttaggttcta ttttactggc gatggccagt ccgcaggcgg ggatggcggc gctgaccggt
      901 acactggcgg gaacgcgcca ggggatgatc agtttcaccc agcaaaatga acaggaagcg
      961 gaccgcattg gtattcaggt gctgcaacgc tcgggattcg atccacaggc gatgccgacc
     1021 ttcctggaaa aattgctcga tcaggcgcgt tactcctcgc gcccgccgga aattttattg
     1081 actcacccgt tgccggaaag tcgtctggca gatgcccgca accgtgctaa tcagatgcgc
     1141 ccgatggtgg tgcagtcgtc ggaagatttc tatctggcga aagcgcgcac actggggatg
     1201 tataattccg gacgtaacca gctcaccagt aatttgctgg atgaatgggc gaaaggaaac
     1261 gttcttcagc aacgagcggc gcaatatggt cgtgctttac aggcgatgaa agccaataaa
     1321 tacgacgagg cgcgtaaaac gctgcaaccg ttactggcgg cagaacctgg caacgcatgg
     1381 tatctcgatc tggctaccga tatcgatctt gagcaaaaca aagccaatga ggcaatcaat
     1441 cgcctgaaaa atgcccgcga tttacgcacc aatccggtgt tgcagctcaa cctggcgaac
     1501 gcttatctgc aaggcggtca accacaagaa gcggccaata ttctgaatcg ctacaccttt
     1561 aataataaag atgacagcaa cggctgggat ttgctggcac aggcggaagc cgcgctaaat
     1621 aaccgcgatc aggagctggc tgcgcgagca gaaggttatg cgctcgccgg acgactcgat
     1681 caggccattt cgctgttgag tagcgccagt tcgcaggtga aattaggcag cctgcaacaa
     1741 gcgcgttacg atgcgcgcat cgaccagttg cgccagctgc aggaacgctt taagccttat
     1801 accaagatgt aattcgcatt aaaggaagaa caatgaccaa acaggtaaaa atctaccata
     1861 acccacgctg ttcaaagagc cgggaaacgc tgaacctgct gaaagaaaac ggcgtggagc
     1921 cagaagtggt gctctacctt gagacacccg ccgatgcggc aacgctgcgc gatttgctga
     1981 aaatgctggg gatgaatagc gcccgtgaat tgatgcgcca gaaagaggat ctttataaag
     2041 aactaaacct ggctgacagt tcgcttagcg aagaggcgct gattcaggca atggtggata
     2101 atccgaagct gatggagcgc ccgattatgg tcgcgaatgg caaagcgcgg attggtcgtc
     2161 caccggagca ggtactggaa atcgtgggtt aagatgtgct ccatcgctag ttgaagcaca
     2221 ttgccggatg cgacgcttgc cacgtcttat ccggcctacg aatactatgg attaacttcg
     2281 cgtaggtcag gcgttcgcgc cgcatccgac aataaacacc ttatctacaa cttcagaatt
     2341 tctttcacaa acggaatggt cagcttacgt tgcgcggtaa tcgacgcacg atccaactga
     2401 tccaacgtca taaatagcgt gcgcatttct ctgtcgagcc gcttcagcaa gaaacgcccc
     2461 acatcttccg gcagttcaaa accacgcaaa cgcgcgcgta actgtagcgc ctgcaactta
     2521 tcttcatcag aaagtggctg caatttgtag atctgccccc agtcgagacg cgacgcgaga
     2581 tccggtaatc ccagattcaa ctgccgcggt ggacgatcgc cggtgatcaa caaccgtgtt
     2641 ttgcccgatt ccagaattcg attgtagaga tcgaaaatcg ccatctccca caactcatcg
     2701 cctgcaatac actcaatgtt gtcgatacag accagcgaca aatgctccat accgtcgagc
     2761 acttccggaa caaaccaggt gcgtttatcc agcgggacat agcccaccgc atcgccacgc
     2821 tgcgacaatt ccgcgcaagc cgcgtgcagc agatggctgc gccccgcgcc ttcgcgtgcc
     2881 cagagataga tgtaaccgct atgttcctga cgcagcacgt tttgcagcgc ggccagtaaa
     2941 gaggagttat cccccggcca gaaacttgca aaggtttcgt cgtcaggaag ataaagtggc
     3001 aaagagagct gtgccggtgt gttcagagat acctcaacca ggatttcaca aaatcgcgag
     3061 aagtttacca cagaatccca tgatgtttga accgggcagc aacactgccc ggtcggtaca
     3121 ttatttgtct gttatatccg cgtcttctgc gtccagcacc acttcttcag ggcgcagcac
     3181 actgatcagt ttgaaaatca aacttaagcc gataccgacg atggtcgcca gcgccatacc
     3241 ttttaactcc gccgcgccga tgtttacctt cgcgccactg acgccgatga tcaaaatcac
     3301 ggaagtcagg atcaggttct gtgctttgtt gtaatccact ttcgattcga tcaaaacacg
     3361 aataccggaa gcaccaatga caccataaag cagcagcgaa acgccgccca tcaccggcaa
     3421 tgggatcatc tggatagcgg cagccagttt accgacgcag gaaagcagga tagcgaaaat
     3481 cgccgccccg ccgataaccc aggtactgta aacacgggtg atcgccatca cgccaatgtt
     3541 ttctccgtaa gtggtatttg gcgtagagcc aaagaagccg gagatcacgg tcgacaggcc
     3601 gttagcaaac atcgaacggt gcagacctgg atcgcgcagc agatcttttt tgacgatatt
     3661 agccgttact accaggtgcc ctacgtgttc ggcaataacc actaacgccg ctggcagaat
     3721 agtcagaatg gcaaaccact cgaagcgcgg cgtatagagg gttggcagtg caaaccagtg
     3781 agcattaata atcggcgtgg tatcgacaat tcccattgcg aaagagagcg cgtaccccac
     3841 cagcacgcca attaaaatcg ggataattgc caggaaacca cgaaacagca cggaacctaa
     3901 aaccgtgacc gccagggtgg taatagagat gatgatggtt ttggagtttg gcgtttgccc
     3961 ttcagccggg agtaaacccg ccataccggc agctacgccc gccagctcca gaccgatgac
     4021 ggcaacgatt gcgcccattg ccgcaggtgg aaacagcacg tccaaccagc cggtccccgc
     4081 tttcttcacg ataaaagaaa ccaggcagaa cagcacgccg cacataataa agccgcccag
     4141 cgcgacttca taccctaacg gcaacagtaa caataccggt gaaataaagg caaagctgga
     4201 accaagataa gccggaattt tccctttaca gatgaagaga tacagcagcg ttccaatacc
     4261 gttaaataac agtacagtcg ccgggttaat atgaaataag acgggcacca ggacggttgc
     4321 accaaacatg gcgaacaaat gttgcaaact aagcgggatt gtctgtaaaa gtggcggtct
     4381 ttcactcacc ccgatagcac ggcgcgtcat agtattatcc tctgtattat gtgttatagg
     4441 cgctttactc aaaaaaaagc cgactcttaa agtcggcttt aattattttt attctttatt
     4501 tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg aataatgtat ccgtgctcgt
     4561 tcagtccctg gtcaatcgat gcagtataca gttcgacgtc cgggtgcgct ttttccagcg
     4621 cagcgatacc ttctggcgca gcgaccagca ccagaacttt gatgctgctg cagcccgctt
     4681 ttttcagcag gtcgatggtc gcgataacgg aaccaccggt tgccagcatt gggtcaacga
     4741 tcagcgccat acgctcatcg atgttagaaa ccagtttctg gaagtacggt accggctcca
     4801 gcgtttcttc attacggtac ataccgacaa cgctgatgcg cgcgctcgga acgttttcca
     4861 gcacaccgtc catcatacca agacccgcac gcagaattgg cacaacggta attttcttac
     4921 ctttgatctg gtcgatttct accgggccgt tccagccttc gatagttact ttttccgttt
     4981 cgaggtcggc ggtcgcttcg taagtcagca ggctacccac ttcggaagcg agttcgcgaa
     5041 agcgcttggt gctgatatct tgctcacgca tcagtcccag cttgtgtttg acgagtgggt
     5101 gtttgacttc cacgatcttc atactctttc tcctttgagg ggcagccaca aaaaaaatcg
     5161 aaggattata cctcctttct tcaaggcggc aatattattt tcgttgactt tagtcaaaat
     5221 gataacagtt tgagataaag ttattttata ttcaaatggt tatgaaagaa gattattcca
     5281 tccgaaaact aacctttgcc ctggcacaag tcttctttcg ccgcgcgccc ggggaaaaga
     5341 cgtgcaaaaa ggttgtgtaa aacagtctcg caaacgtttg ctttccctgt tagaattgcg
     5401 ccgaatttta tttttctacc gcaagtaacg cgtggggacc caagcagtga ccgataaaac
     5461 ctctcttagc tacaaagatg ccggtgttga tattgacgcg ggtaatgctc tggttggaag
     5521 aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg atgggcggtc tgggcggctt
     5581 cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaaccc gtgctggttt ccggcactga
     5641 cggcgtaggt accaagctgc gtctggcaat ggacttaaaa cgtcacgaca ccattggtat
     5701 tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa ggtgcggaac cgctgttttt
     5761 cctcgactat tacgcaaccg gaaaactgga tgttgatacc gcttcagcag tgatcagcgg
     5821 catcgcggaa ggttgtctgc aatcgggctg ttctctggtg ggtggcgaaa cggcagaaat
     5881 gccggggatg tatcacggtg aagattacga tgtcgcgggt ttctgcgtgg gcgtggtaga
     5941 aaaatcagaa atcatcgacg gctctaaagt cagcgacggc gatgtgctga ttgcactcgg
     6001 ttccagcggt ccgcactcga acggctattc gctggtgcgc aaaattcttg aagtcagcgg
     6061 ttgtgatccg caaaccaccg aacttgatgg taagccatta gccgatcatc tgctggcacc
     6121 gacccgcatt tacgtgaagt cagtgctgga gttgattgaa aaggtcgatg tgcatgccat
     6181 tgcgcacctg accggcggcg gcttctggga aaacattccg cgcgtattgc cagataatac
     6241 ccaggcagtg attgatgaat cttcctggca gtggccggaa gtgttcaact ggctgcaaac
     6301 ggcaggtaac gttgagcgcc atgaaatgta tcgcaccttc aactgcggcg tcgggatgat
     6361 tatcgccctg cctgctccgg aagtggacaa agccctcgcc ctgctaaatg ccaacggtga
     6421 aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc gaacaacgcg tggttatcga
     6481 ataatgaata ttgtggtgct tatttccggc aacggaagta atttacaggc aattattgac
     6541 gcctgtaaaa ccaacaaaat taaaggcacc gtacgggcag ttttcagcaa taaggccgac
     6601 gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa cgcatacgct catcgccagc
     6661 gcgtttgaca gtcgtgaagc ctatgaccgg gagttgattc atgaaatcga catgtacgca
     6721 cccgatgtgg tcgtgctggc tggttttatg cgcattctca gcccggcgtt tgtctcccac
     6781 tatgccgggc gtttgctgaa cattcaccct tctctgctgc cgaaatatcc cggattacac
     6841 acccatcgtc aggcgctgga aaatggcgat gaagagcacg gtacatcggt gcatttcgtc
     6901 accgatgaac tggacggtgg cccggttatt ttacaggcga aagtcccggt atttgctggt
     6961 gatacggaag atgacatcac cgcccgcgtg caaacccagg aacacgctat ttatccactg
     7021 gtgattagct ggtttgccga tggtcgtctg aaaatgcacg aaaacgccgc gtggctggat
     7081 ggtcaacgtc tgccgccgca gagctacgct gccgacgagt aatgcccccg tagttaaagc
     7141 gccagctctg ccgctggcgt ttttcaattc acctgtaaat cgcaagctcc agcagttttt
     7201 ttcccccttt tctggcatag ttggacatct gccaatattg ctcgccataa tatccaggca
     7261 gtgtcccgtg aataaaacgg agtaaaagtg gtaatgggtc aggaaaagct atacatcgaa
     7321 aaagagctca gttggttatc gttcaatgaa cgcgtgcttc aggaagcggc ggacaaatct
     7381 aacccgctga ttgaaaggat gcgtttcctg gggatctatt ccaataacct tgatgagttc
     7441 tataaagtcc gcttcgctga actgaagcga cgcatcatta ttagcgaaga acaaggctcc
     7501 aacgctcatt cccgccattt actgggcaaa attcagtccc gggtgctgaa agccgatcag
     7561 gaattcgacg gcctctacaa cgagctattg ctggagatgg cgcgcaacca gatcttcctg
     7621 attaatgaac gccagctctc cgtcaatcaa caaaactggc tgcgtcatta ttttaagcag
     7681 tatctgcgtc agcacattac gccgatttta atcaatcctg acactgactt agtgcagttc
     7741 ctgaaagatg attacaccta tctggcggtg gaaattatcc gtagcgatag catccgttac
     7801 gcgctgctgg agatcccatc agataaagtg ccgcgctttg tgaatttacc gccagaagcg
     7861 ccgcgtcgac gcaagccgat gattcttctg gataacattc tgcgttactg ccttgatgat
     7921 attttcaaag gcttctttga ttatgacgcg ctgaatgcct attcaatgaa gatgacccgc
     7981 gatgccgaat acgatttagt gcatgagatg gaagccagcc tgatggagtt gatgtcttcc
     8041 agtctcaagc agcgtttaac tgctgagccg gtgcgttttg tttatcagcg cgatatgccc
     8101 aatgcgctgg ttgaagtgtt acgcgaaaaa ctgactattt cccgctacga ctccatcgtc
     8161 cccggcggtc gttatcataa ttttaaagac tttattaatt ttcccaatgt cggcaaagcc
     8221 aatctggtga acaaaccact gccgcgttta cgccatattt ggtttgataa agcccagttc
     8281 cgcaatggtt ttgatgccat tcgcgaacgc gatgtgttgc tctattatcc ttatcacacc
     8341 tttgggcatg tgctggaact gctgcgtcag gcttcgttcg acccgagcgt actggcgatt
     8401 aaaattaaca tttaccgcgt ggcgaaagat tcacgcatca tcgactcgat gatccacgcc
     8461 gcacataacg gtaagaaagt cactgtggtg gttgagttac aggcgcgttt cgacgaagaa
     8521 gccaacattc actgggcgaa gcgcctgacc gaagcaggcg tgcacgttat cttctctgcg
     8581 ccggggctga aaattcacgc caaactgttc ctgatttcac gtaaagaaaa cggtgaagtg
     8641 gtccgttacg cacacatcgg gaccgggaac tttaacgaaa aaaccgcgcg tctttatact
     8701 gactattcgt tgctgaccgc agatgcgcgt atcaccaacg aagtacggcg ggtatttaac
     8761 tttattgaaa acccataccg cccggtgaca tttgattatt taatggtgtc accgcaaaac
     8821 tctcgccgtc tgttatatga aatggtagac cgcgaaatcg ccaacgcgca gcaagggctg
     8881 cccagtggta tcaccctgaa gctaaataac cttgtcgata aaggcctggt tgatcgtctg
     8941 tatgcggcct ccagctccgg cgtaccggtt aatctgctgg ttcgcggaat gtgttcgctg
     9001 atccccaatc tggaaggcat tagcgacaac attcgtgcca tcagtattgt tgaccgttac
     9061 cttgaacatg accgggttta tatttttgaa aatggcggcg ataaaaaggt ctacctttct
     9121 tccgccgact ggatgacgcg caatattgat tatcgtattg aagtggcgac accgctgctc
     9181 gatccgcgcc tgaagcagcg ggtgctggac atcatcgaca tattgttcag cgatacggtc
     9241 aaagcacgtt atatcgataa agaactcagt aatcgctacg ttccccgcgg caatcgccgc
     9301 aaagtacggg cgcagttggc gatttacgac tacatcaaat cactcgaaca acctgaataa
     9361 ccctatgcca atacacgata aatcccctcg tccgcaggag tttgctgcgg tcgatcttgg
     9421 ttcaaacagt tttcacatgg tcatagcccg tgtggtagat ggtgccatgc agattattgg
     9481 ccgcctgaaa cagcgggtgc atctggcgga cggcctgggg ccagataata tgttgagtga
     9541 agaggcaatg acgcgcggtt tgaactgtct gtcgctgttt gccgaacggc tacaagggtt
     9601 ttctcctgcc agcgtctgta tagtcggcac ccatacgctg cgtcaggcgc tgaacgccac
     9661 tgactttctg aaacgcgcgg aaaaggtcat tccctacccg attgaaatta tttccggtaa
     9721 tgaagaagcc cgtctgattt ttatgggcgt ggaacatacc caaccggaaa aaggtcgcaa
     9781 actggttatt gatattggcg gcggatctac ggaactggtg attggtgaaa atttcgaacc
     9841 tattctcgtt gaaagccgcc ggatgggttg tgtcagcttt gcccagcttt attttcctgg
     9901 cggggtcatc aataaagaga attttcagcg cgctcgcatg gcggcagcac aaaaactgga
     9961 aactttaacc tggcaattcc gtattcaggg ctggaacgtt gcaatgggcg cttccggtac
    10021 cataaaagcc gcccatgaag tgttaatgga aatgggcgag aaagacggga taattacccc
    10081 ggaacgtctg gaaaaactgg taaaagaaat tttacgtcac cgtaatttcg catcgctgag
    10141 tttaccgggt ctttccgaag agcggaaaac agtcttcgtt cctggactgg cgattttatg
    10201 tggtgtgttt gatgctttag ccatccgtga actgcgcctt tctgacgggg cgttacgcga
    10261 aggcgtactg tatgaaatgg aaggacgttt ccgtcatcag gatgtgcgta gtcgcaccgc
    10321 cagcagcctc gccaaccagt atcacataga cagcgaacag gcccgacgag tgctggatac
    10381 cactatgcaa atgtacgaac agtggcggga acagcaaccg aagctggcgc atccgcaact
    10441 ggaggcgcta ctgcgatggg ccgccatgct gcatgaggtc gggttgaata tcaaccacag
    10501 cggtttgcat cgccactccg cttatattct gcaaaacagt gacttgccgg gttttaatca
    10561 ggaacagcag ctgatgatgg cgacactgct gcgctatcac cgtaaagcga ttaagctcga
    10621 cgatctgccg cgctttacct tgtttaagaa gaaacagttc ctgccactga tacagctatt
    10681 gcgccttggc gtattactaa acaatcaacg tcaggcaacc accacaccgt caacattgac
    10741 actgattact gatgacagtc actggacact gcgtttcccg catgactggt ttagtcagaa
    10801 tgcgctggta ctgcttgatc tggaaaagga gcaagaatac tgggaaggcg tggctggctg
    10861 gcggttgaaa attgaagaag aaagtacccc agaaatcgcc gcttaatgct ttgcgggccg
    10921 acgagaaatg tcggcccgca ttattcaggc actttcgcga atgggttcga tttcattcag
    10981 cgtatcaatt aacggttgcg gcttaccaat aagataaccc tgcatataat cgatccccaa
    11041 agagagcacc gcctcgcgga tctcttcgtt ttcaacgtac tctgccacta cccgcatttt
    11101 cttcattcgc gccagatggc aaatcgacgc cacaatctga taatccagac tatttgacac
    11161 aatattgcgg ataaagctgc cgtcaatttt aagcagatcg gcattcacat ttttaagccg
    11221 tgcatagctg gcgtagccgg tgccgaaatc atcaatcgca atctggcagc ctaattcctg
    11281 aagatgctgc aaggtgattt gcgcctgctt aacattggtc agagcattac tttcggtgac
    11341 ttcaaaaata agttgccacg cttcaatctg atatttagcc agcaactgac tgacttcaac
    11401 aggaaaacga gcctgacata ccgaggttgg agacagatta atagcaaaac ggtgagcggg
    11461 catcttcgct ctgttttcag ccataaattg cagcgtatgc tcaatgaccc acatgtcgat
    11521 actcgacgat aaaccaaatt cgtgcgcgac cggcaagaag ctatcggggc cgatcagttc
    11581 atcattctca cctttcatgc gcagaagaat ttcatggtaa acatcaccac gcataccggt
    11641 aatcggctgg gccatcagga aaaaatggtt gtgctccagc gcctgctgta gtcgattcat
    11701 catcgcgact ttatctttca attcgcgttg caaatacatt gccccgcaac gctgcatatt
    11761 ttccggggcg ttggtcacga tggaaagttc ggcgaccgta tttagctctc ccagcagcag
    11821 gtagatatga ttcactggcg agcgcacata gcagtaactg acgccaatct gcggttgcat
    11881 cggcatccca tcccaaaaga aacgaaattg cttgagatga ctatccagtg cggtaatgcg
    11941 ctcctggtgc gattctgtat tcagtcgcag cgcgagatcg ttacccgaaa gctgataaac
    12001 atcttcaccc ggttccagca agggtgacag ccagtgagaa agtttttgct tgt
//
LOCUS       DADLTF010000121        11847 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.121, whole genome shotgun sequence.
ACCESSION   DADLTF010000121 DADLTF010000000
VERSION     DADLTF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.121"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1019
                     /locus_tag="JN749_003136"
     CDS             <1..1019
                     /locus_tag="JN749_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7369463.1"
                     /translation="ELEEKWLDLKRMEISANAFNRYESVARTMVPKIGGGRLVSSVTK
                     EELLYIRKDLLTGYQNSTKNKAAAKGRSVVTVNYYMTTIAGMFQFAADHGYLEANPFQ
                     GIKPLKRARAEPDPLTRDEFIRLIDACRHQQTKNLWSLAVYTGMRHGELVSLAWEDID
                     MKAGTITIRRNYTKLGEFTLPKTEASTNRVVHLIQPAISVLKNQAEMTRLGKQHHIKV
                     QLREYGRSVNHECTFVFNPQVVRKSKQVGFIYKVDSIGDSWETAIKRAGIRHRKAYQS
                     RHTYACWSLSAGANPSFIASQMGHASAQMVFNVYGAWMTDSNAEQIAMLNQKLADYVP
                     WSPK"
     gene            1032..1802
                     /locus_tag="JN749_003137"
     CDS             1032..1802
                     /locus_tag="JN749_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAY7369464.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            1855..2250
                     /locus_tag="JN749_003138"
     CDS             1855..2250
                     /locus_tag="JN749_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369465.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2291..3034
                     /gene="cmoA"
                     /locus_tag="JN749_003139"
     CDS             2291..3034
                     /gene="cmoA"
                     /locus_tag="JN749_003139"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HAY7369466.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3031..4002
                     /gene="cmoB"
                     /locus_tag="JN749_003140"
     CDS             3031..4002
                     /gene="cmoB"
                     /locus_tag="JN749_003140"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HAY7369467.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4167..6596)
                     /locus_tag="JN749_003141"
     CDS             complement(4167..6596)
                     /locus_tag="JN749_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HAY7369468.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMAAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6621..7721)
                     /locus_tag="JN749_003142"
     CDS             complement(6621..7721)
                     /locus_tag="JN749_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HAY7369469.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="JN749_003143"
     CDS             complement(8109..8855)
                     /gene="cutC"
                     /locus_tag="JN749_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAY7369470.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8869..9435)
                     /locus_tag="JN749_003144"
     CDS             complement(8869..9435)
                     /locus_tag="JN749_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAY7369471.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9651..11384
                     /gene="argS"
                     /locus_tag="JN749_003145"
     CDS             9651..11384
                     /gene="argS"
                     /locus_tag="JN749_003145"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAY7369472.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            11561..>11847
                     /locus_tag="JN749_003146"
     CDS             11561..>11847
                     /locus_tag="JN749_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HAY7369473.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         2939 a         3005 c         2866 g         3037 t
ORIGIN      
        1 aggaacttga agaaaagtgg ctggatctga aacgaatgga aatctctgca aatgcattca
       61 atcgctatga atccgttgca agaacgatgg ttccgaaaat tggaggcggc agactggtgt
      121 catcggtaac caaagaggaa ttgctgtata tcaggaaaga tttgctgacc gggtatcaga
      181 attcaacgaa aaacaaagca gcagcaaaag gacggagcgt cgttactgta aattattaca
      241 tgacgacaat cgctggaatg tttcagtttg ctgcagatca cggttactta gaagcaaatc
      301 ccttccaggg aattaagcct cttaaaagag ccagggcaga gccagatccg ttaactcgtg
      361 acgaatttat tcgcctgata gatgcttgcc gacatcagca gacgaaaaac ctgtggtcat
      421 tggctgtgta cacaggaatg cgtcacggtg aactggtctc cctggcctgg gaagatatcg
      481 atatgaaagc aggaactatt actatcaggc gcaattatac gaaactcggt gagttcactc
      541 tacctaaaac tgaagcaagt acaaacaggg ttgtgcatct tatccagccc gctatcagtg
      601 tcctgaaaaa tcaggctgaa atgacaagac tgggtaagca gcaccacatc aaggttcaac
      661 tacgtgaata tggacgttca gtgaatcatg aatgtacttt cgtatttaac ccccaggtgg
      721 ttagaaaaag caaacaggta ggttttatct acaaggtaga ttccattggc gactcatggg
      781 aaacagccat taagcgtgcg ggcatcaggc accggaaagc ataccagtca cgacacactt
      841 atgcgtgctg gtcattatct gccggagcaa acccaagctt cattgccagc cagatgggcc
      901 atgcaagtgc ccagatggtg ttcaatgtat acggagcatg gatgactgac agtaatgcag
      961 aacagatcgc aatgctgaat cagaagctgg cagattatgt cccatggtca ccaaagtgac
     1021 acaagagact tattaaaatc agtgggttag ccttcaatac ccgtcatgtt aactgcgtgg
     1081 agggcaacac cacgctttac gccctgccga aacccgaggt tgtcctgcgc tggcgtgaac
     1141 agaccacaga tgacttccgc ttctgtttta agtttccggc gaccatttcg catcaggcag
     1201 cattacgaca ttgcgatgat ttagtgactg aatttttgac ccgcatgtca ccgttggctc
     1261 cgcgcattgg gcaatactgg ctgcaactgc ctgccacatt cggcccacgg gagctgcctg
     1321 cgctttggca ttttctcgat tctctccctg gcgaatttaa ttatggtgtg gaagtccgcc
     1381 atccacagtt tttcgccaaa ggggaagagg aacaaacgct taatcgcggt ttacatcagc
     1441 gcggcgttaa tcgggtgatt ttagacagcc gcccggttca tgcatcacgt ccacacagtg
     1501 aagctattcg cgacgctcaa cgaaaaaaac ctaaagttcc ggtacatgct gtactgacgg
     1561 cgacaaatcc gctgatccgt tttatcggta gtgatgatat gacgcaaaac cgggaattat
     1621 ttcaggtctg gttacaaaaa ttagcgcagt ggcatcagac cactacgcct tatctttttt
     1681 tacatacgcc agatattgcc caggccccgg aactggtaca taccctgtgg gaagacttac
     1741 gtaaaacgct tccagagatc ggagcagttc cggctattcc acagcaatct tctcttttct
     1801 gaatttgcca cctatcatag acaggtgcca tcggccattt taaagggagt ttgtatggta
     1861 agcgcgctat atgccgtttt aagtgcgttg ttattaatga agttctcttt tgatgtcgtt
     1921 cgcctgcgaa tgcagtaccg cgttgcctat ggtgacggtg gttttagcga actgcaaagc
     1981 gctattcgca ttcatggtaa cgcggtggaa tatattccca tcgcgattgt gctgatgctg
     2041 tttatggaaa tgaatggcgc agaaacctgg atggtgcata tttgcggcat cgttttgctt
     2101 gctggtcgtc tgatgcatta ttacggtttt catcaccgtc tgtttcgctg gcgacgttcc
     2161 ggcatgagcg ccacctggtg tgcgctgttg ctgatggtgc tggcgaatct ttggtatatg
     2221 ccctgggagt tggttttctc cctgcgttag cgcacaatac gccactttct ttttcccgga
     2281 tttttacgtt atgtctcacc gcgacacgct attttctgcc cctatcgcca gactgggcga
     2341 ctggaccttt gatgaacggg tagctgaagt cttcccggat atgatccagc gttccgttcc
     2401 cggctattcc aatattattt ccatgattgg tatgttagcc gagcgcttcg ttcaacctgg
     2461 tacgcaggtt tacgatctgg gttgttctct gggcgcggcg acgctctcgg tgcgtcgcaa
     2521 cattcatcat gataattgca aaattattgc catcgacaac tccccggcga tgattgaacg
     2581 ctgccgtcgt catattaacg cctataaagc ccctacgcca gtagacgtca ttgaaggtga
     2641 tattcgcgat atcgccattg aaaacgcatc gatggtggtg ctgaatttta ccctgcaatt
     2701 cctggaacct tccgagcgcc aggcattact ggataaaatt tatcaaggac tgaacccggg
     2761 cggcgcgctg gtgctttcgg aaaaattcag tttcgaagat gccaaagttg gtgaactgtt
     2821 gttcaacatg caccacgact ttaaacgtgc caacggttac agcgaactgg agatcagcca
     2881 gaagcgcagc atgctggaaa acgtgatgct gaccgattcc gtggaaaccc ataaagcacg
     2941 cctgcataaa gccggttttg agcatagcga gctgtggttc cagtgcttta actttggttc
     3001 actggtggca ttaaaggcag aggacgctgc atgatcgact ttggtaactt ttattctctg
     3061 attgccaaaa atcatctttc acactggctc gaaacgctgc ccgcgcagat tgctaactgg
     3121 cagcgcgagc agcagcacgg gctgtttaag cagtggtcca acacggtgga atttctgcct
     3181 gaaattaaac cgtatcgtct ggatttattg catagcgtaa ccgccgaaag cgaagagcca
     3241 ctgagcaccg ggcaaattaa acgcattgaa acgcttatgc ggaacctgat gccatggcgc
     3301 aaagggccgt tctcactgta tggcgtcaac atcgataccg aatggcgttc cgactggaaa
     3361 tgggatcgtg ttctgcccca tctttctgat ttaaccggac gcaccattct ggatgtcggc
     3421 tgcggcagcg gttatcacat gtggcgcatg attggcgcag gcgcgcacct cgcggtgggg
     3481 atcgacccta cgcaactgtt tctgtgccag tttgaagcgg tgcgtaaact actgggtaac
     3541 gatcagcgcg cacatttgtt accgttaggt attgaacagc ttccggcact gaaagccttt
     3601 gataccgtct tttcgatggg cgtgctttac catcgccgtt cgccgctgga gcatctctgg
     3661 cagttgaaag atcaactggt gaatgagggt gaactggtgc tggaaacgct ggttattgat
     3721 ggcgatgaga acacggtgct ggtaccaggc gatcgttacg cgcaaatgcg taatgtctat
     3781 ttcatccctt ccgcgctggc gctgaaaaac tggctgaaga agtgtggttt tgtagatatc
     3841 cgcattgtgg atgtgtgcgt taccactact gaagagcagc gacgcactga atggatggtt
     3901 accgaatctc tgtccgattt cctcgaccca catgatccga gtaagacagt tgaaggttat
     3961 cctgcgccta aacgcgcggt gctgattgcg cgcaagccgt aaaggtctgg tattactgcc
     4021 ggatgcggcg tgaacgcctt atccggccta caaacccttg ctaattcaat ctattgcagg
     4081 gactatgtag gcctgataag catagcgcat caggcagttt tacgtttgca taacctcagc
     4141 gcccgtttcc gggcgctatt cacttcttac gcctgaacag caggcttatc aaatgccgtt
     4201 aacttcggtg cgttacccgt atatttttcg atatacacca gcgcagagtt accggcacag
     4261 gcatttgcca gctgcgagct ggggatatcc gccgttaaca cgttcgcact gccgttttta
     4321 cacaagccgt tttccagatc tggccatgca ccttcatgca cgcataccac gccttttttg
     4381 atcccgtcag tcaccaccgc gcctgtcaaa atctgaccac gtttgttcca gacacgcacc
     4441 agatcgccat tcgcaatacc aaatcgagca gcatcttcgg tgtgaatagt gatcggttca
     4501 cgatcggcaa ccgcatattt tttacgcagt tccgcatagt taagctgact gtgtaaacgg
     4561 tgtgccggat gtgcggtcag aagctgcaac tgcttctcgt cggcggtacc cttccactca
     4621 tcaggcgcaa gccaggttgg gtgtgccggg caatccttat agccaaattt ttccagcgtt
     4681 ttggagtaaa tctcaatttt gccgcttggc gtacccagcg catttttcac cggatcggcg
     4741 cggaaatcac cataacgaac gtactgttcg ttcttctcgc tgcggcgcat ttcgatcagt
     4801 ttattttgct gccagaaggc attaaacatt ggcatagtga cgcgttgcgc acgggcccct
     4861 ttctgagcgg catcatagaa aaatttcagc cacgccattt catctttacc ttcggtatag
     4921 atctcttttc cgccaggttt gagtaattcc gcaagatcgg caaacacgtc aaaatcgtta
     4981 cgcgcttcaa attgcggagc aacagcctgc ttcatcggca caatatgctg gttgctgtaa
     5041 tcaccggtca tcgtcaagtc attgcgctca aacgatgtgg tgatcggtaa tacgatatct
     5101 gcgtgtttgg ctgctgcggt ccagtagcat tcagaaacga cgatcatctc cggtttctgc
     5161 caggctttga tcagacgatt agtatcctgg tgatgggtaa agttaccgcc acccgcccac
     5221 cagatcattt tgatattcgg atacgtttgt tctttaccgt tatgctgata tttgccgcca
     5281 ggattttcca gcgcatcgac gatacgggca acaggaatcg ccgccattcc gccatcatca
     5341 gcggcctcgc tggcctgtcc ggcaatcgcc gcagacattt ccggcagcac gccgccaaca
     5401 cgcatcgggt tgccaccatt ggagtaatga taagagaaac caaaaccgcc gcccggtgta
     5461 ccaatctgcc ctaacatcgc cgccagcgtc accagcatcc agtgtttctg ttcaccatat
     5521 tgctggcgct gaattcccca gcctgccatc agaattgtgc gattcgccgc catcagttca
     5581 gcgagtttga ctatctgcgc ttccggtacg ccagtaattt ccgccgccca gaccgcgctc
     5641 ttcggcgtat tatcgctctt acctgtcaga tactcttcaa actgcggata cccggtagtg
     5701 tatttttcaa ggaacacttt atcgtgtttg ccttgtgtca tcagggtatg cgcaatccct
     5761 aacatcagtg ccacgtcggt gcccatattc ggcgcgatcc aggtggcatt atcgccaaag
     5821 aattcgatgg tttcggagcg gataggatca atggcaatca ctggtttgcc agattttttc
     5881 agctgatgga agtattccag cccttgctca tcggtactgc tccaggcaat ttttaaggta
     5941 ttcagcgggt tcattcccca cagcaccaca acctggctgt tttccagaat cagcggccag
     6001 gaggcctgct gttcatacac ctctacagaa ccgaccacat gcggcatgat cacctgtgct
     6061 gccccggttg aataatcgcc gctatgcccg gaataaccgc ccgccaggtt catataacgt
     6121 tgcagtaagg tttgcgcttt atgcaacacg ccagaagagc gccagccgta agatccggca
     6181 aaaatggccg atggtccgtt agctttacga atacgctcat gttgctcatg aatcagtttt
     6241 aatgcctgtt cccagctcac ctgtacatag gtatcttcgc cacgaccttt cgccggttgc
     6301 agtggattgt cgagatagct ttttctcacc atcggatgct gaatacgcgc cgtggtgtgt
     6361 acctgatccg ccgccgtaga ctgtaaggag ttcggtatgg ttttcgccag cgcgcctgtc
     6421 gaagaaacaa tcttgccgtc cttcacttct acgctcatcg ctccccaacg tcccgcggtg
     6481 aggattttac cgcccttctc ttctgcccat gcggggagcg gtgctgccga tgtcaccacc
     6541 agcgctccag cggcaatacc gctgtgttta ataaattcac gtcttgttaa tgccataact
     6601 tcctccctga tcaacgagga tcactgtttc tcggtaatat ctttggcgtt gtactggaaa
     6661 taccgcgtta aaatgtccag ttcgttttcg ctcatgctgg ttcgtgcccc cattcctttg
     6721 gcaatggacg gccacgcatt gacggtgtaa tggtcggcgg caatagaggc atgacaacca
     6781 gcgcaatagg tatcgtcaag tttttcagcg tattgccata gcggtttacg gtccgctaat
     6841 gcgggatcgg taagcgcacc ctgtaaagac gcctgacgcc attgattgcc gtattcgtca
     6901 gcctgccatt ccccgtttac cgtgagcgcc ttgatacctt cttcacttaa tgtggctagc
     6961 gccagccgtt gacctgccgc caggtagagc gtgttttcac tgccctgcat ttgataaccc
     7021 tgcaacagaa cgatcggctg tttgccactg gcatcaacga cggtgagatc ggtaccagga
     7081 ttcacggtag ccagctcgcc tatgcgagaa gttttgaaag gataaatatg tgcgccatta
     7141 gtcactgaag tagcggcctg actttccagc tcatgcgccg cgttgtcatc catttttatt
     7201 tctggcggaa aatgggcaat gcctttatga caatcgatac aggtttcgcc gcccttttgt
     7261 gctttgttat gcattttctg cgcagattca ctttgcgagg caatatccat ggcatcaaaa
     7321 gaatggcaac tacggcacgt tgcagagtca gttgctttta attctttcca tactgtttcg
     7381 gccatttcct ggcgatgagt ttcgaactta tcgtcactgt ctattttgcc gctaacaaat
     7441 tcatgataaa tatctttaga tgcttttaat ttagcaaata aataatccat ccctgacttt
     7501 ggaatatggc aatcggcaca ttccgcacgt atccctttct ggttcgaaaa gtggacagtt
     7561 ccctgatatt cctcaaaagg tttactcatc gagtggcagg aaaggcaaaa tgctgtatcc
     7621 gacgttttat gtaaggcttt ttgcgccagc aataacccgc cgccaccaac cacaaccgct
     7681 atcagcaaaa acaataaccc aatgcgtttt ttccctcgca tagattcctc taaataaatt
     7741 ccagaacacc gaaatcattg tataattaaa tacacaacga attaaccgct tgcataactc
     7801 ggcacaacac tgcctgaaat aatcgataaa gaatatgatt tattacaatg taatcattaa
     7861 ttgctaagga ataacccagt tgctatttag aatatatatt tatcagtttt agcaatttaa
     7921 atcccataat caatgtgaat ataaaaaata tttttagcac aacgctatgc gcactgtcac
     7981 ccgctcaaca gatcacggtt atcgttcgtt ttttatactg ctcagggata aaaaaaggcc
     8041 cctgttgaaa ttgcaggggc ctggtacgag caagcatcat attgggcgac atgatgcaac
     8101 ggtaaaaatc atttggcctg atggcgttca atgattcctt tcatttcagc aaccgccgcc
     8161 ccgtctacga tataacgcga atactcgtcc gcgtgttcat ctgatgacat ggacaatcct
     8221 tgattacgat aacgcatcgg tgaggcttgc cacgctcccg cggagctatg gacttccagc
     8281 actccggcat cgaggaagtg gtgcaggttt tctgcacgga ctcctgctcc agccataatg
     8341 attggagcat cacgatgggc aataagttcc ataatttttg ataaaccttg cagtgcgtct
     8401 gatttttgcc ctgatgtcag tacccgggca atgcccaatt ccgcaagatt attgagtgta
     8461 tataaagggt tagcgcacat atcgaaggcg cgatgaaaag tcactgccag cggaccggca
     8521 gcagccatta ttttttccat tcgtggcata tcgacattcc catcaacatc gagaacgccc
     8581 gtcaccagtc caggaaaacc taattcgcgg accgtgcgca catcctcaag aatggcggca
     8641 aactcaccgt cgctgtagca aaaatcacca ccgcgtgggc gaattatcgg atgcacaggg
     8701 atcgtcaccc gctggcgcac ggatttcagt ccccccaacg acggcgttaa gcccccctct
     8761 tttggggctg cgcataattc aactctgtct gcgccgtttt gctgcgccgt tagtgcacat
     8821 tccatgctgt aacagcaaat ttccagtaat gccatttttg ctccttaatt acgctgactg
     8881 ctcgctggca acgatctctt caatggacca cggatgaaac ttaatagttg ttttgccatc
     8941 ggtgaccgcc agcgtcggat tcggtaatcg ctcatgctca ccttttggtg aactggtttt
     9001 aactgagatc cccggtaggc tgagtccttc atcagaaagc aatgccagcg cacgggcatt
     9061 cagcgtttct ggatcacccg gcagaacgat ttcaatatgt tcccaacctt cgtgtgggta
     9121 acgtttttcc ccaggccacg gtagctccac attggaaaac tgccagtgcg caacctgtac
     9181 cggttcatgc aatttaaaca gacaaatcgg tctgccattg atcatttttt ctgacaaaag
     9241 ctcgccacac tgttcaaaac cgcgacgcca gcgctcagcg gtgacgtttt gatggcaacg
     9301 caaagaaatg tgatcggcag tcagcggagt gatatccaac cccagacggc gggaaagttc
     9361 atctaatgcg tgggtaaatc gcggtaaatc cgatgcaata tcctgcagct cgtcgataga
     9421 ttgccagttc gccataatca ctcttcgtct ttcagtaaaa gcgttaattt accctgttgc
     9481 cctgtgccaa ccaaccgctg atttcacgcc gcttctgatg caatagtgaa aacggcaata
     9541 cgccacgcgc acgttgctga cgaaaacagc catttgcagt atactcccgc cctaatttct
     9601 ttaactggtg cgggcaattt ttgctcgctt catcaatgta aggtattccg gtgaatattc
     9661 aggctcttct ctcagaaaaa gtccgtcagg ccatgattgc ggcaggcgcg cctgcggatt
     9721 gcgaaccgca gattcgtcag tcagcaaaag ttcagttcgg cgactatcag gctaacggca
     9781 tgatggcagt tgctaaaaaa ctgggtatgg caccgcgaca attagcagag caggtgctga
     9841 ctcatctgga tcttaacggt atcgccagca aagttgagat cgccggtcca ggctttatca
     9901 acattttcct tgatccggca ttcctggctg aacatgttca gcaggcgctg gcgtccgatc
     9961 gtctcggtgt tgctacgcca gaaaaacaga ccattgtggt tgactactct gcgccaaacg
    10021 tggcgaaaga gatgcacgtc ggtcacctgc gctctaccat tattggtgac gcagcagtgc
    10081 gtactctgga gttcctcggt cacaaagtga ttcgcgcaaa ccacgtcggc gactggggca
    10141 ctcagttcgg tatgctgatt gcatggctgg aaaagcagca gcaggaaaac gccggtgaaa
    10201 tggagctggc tgaccttgaa ggtttctacc gcgatgcgaa aaagcattac gacgaagatg
    10261 aagagtttgc cgagcgtgca cgtaactacg tggtaaaact gcaaagcggt gacgaatatt
    10321 tccgcgagat gtggcgcaaa ctggtcgaca tcaccatgac gcagaaccaa atcacttacg
    10381 atcgtctcaa cgtgacgctg acccgtgatg acgtgatggg cgaaagcctc tacaacccaa
    10441 tgctgccagg aattgtggcg gatctcaaag ccaaaggtct ggcagtagaa agcgaagggg
    10501 cgaccgtcgt attccttgat gagtttaaaa acaaggaagg cgaaccgatg ggcgtgatca
    10561 ttcagaagaa agatggcggc tatctctaca ccaccactga tatcgcctgt gcgaaatatc
    10621 gttatgaaac actgcatgcc gatcgcgtgc tgtattacat cgactcccgt cagcatcaac
    10681 acctgatgca ggcatgggcg atcgtccgta aagcaggcta tgtaccggaa tccgtaccgc
    10741 tggaacacca catgttcggc atgatgctgg gtaaagacgg caaaccgttc aaaacccgcg
    10801 cgggtggtac agtgaaactg gcagacctgc tggatgaagc cctggagcgt gcacgtcgtc
    10861 tggtggcaga aaagaacccg gatatgccag ctgacgagct ggaaaaactg gctaacgcgg
    10921 ttggtattgg tgcggtgaaa tatgcggatc tctccaaaaa ccgcaccacg gactacatct
    10981 tcgactggga caacatgctg gcgtttgagg gtaataccgc gccatacatg cagtatgctt
    11041 acacgcgtgt attgtccgtg ttccgtaaag cagaaattaa cgaagagcaa ctggctgcag
    11101 ctccggtaat catccgtgaa gatcgtgaag cgcaactggc agctcgcctg ctgcagtttg
    11161 aagaaaccct caccgtggtt gcccgtgaag gcacgccgca tgtaatgtgt gcttacctgt
    11221 acgatctggc cggtctgttc tctggcttct acgagcactg cccgatcctc agcgcagaaa
    11281 acgaagaagt gcgtaacagc cgtctgaaac tggcacaact gacagcgaag acgctgaagc
    11341 tgggtctgga tacgctgggt attgagactg tagagcgtat gtaatcgatt tttcgtgaga
    11401 gtgaagcctg atcagggtta gccgatcagg cttttttatt gccatctaaa tgtattctga
    11461 aaacggacat gccattgttt tctcactgtt gagtaagagg ccagaagcgt aatatccagg
    11521 cccaggaaaa cgataactgt tcaatttaag gaataccgaa gtgtttaaat ttcttgtatt
    11581 aacattaggc attatctctt gccaggctta cgcagaagat acggttatag taaacgacca
    11641 tgacacttca gccatcaaag attgttggca aaaaaattca gatgatgata ctgacattaa
    11701 cgtgatcaaa tcatgcctgc gacaagaata caatctcgtc gatacgcaat taaataaagc
    11761 ctatggtgaa gcttatcgtt atatagaaca agtgccacgc acaggtgcaa aaaaacttga
    11821 tactgaacaa cttaacttgc ttaaaaa
//
LOCUS       DADLTF010000122        11785 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.122, whole genome shotgun sequence.
ACCESSION   DADLTF010000122 DADLTF010000000
VERSION     DADLTF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.122"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            202..537
                     /locus_tag="JN749_003147"
     CDS             202..537
                     /locus_tag="JN749_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HAY7369474.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            630..713
                     /locus_tag="JN749_003148"
     CDS             630..713
                     /locus_tag="JN749_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369475.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="JN749_003149"
     CDS             813..1634
                     /locus_tag="JN749_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAY7369476.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JN749_003150"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="JN749_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HAY7369477.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JN749_003151"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="JN749_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HAY7369478.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="JN749_003152"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="JN749_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HAY7369479.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JN749_003153"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="JN749_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HAY7369480.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JN749_003154"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="JN749_003154"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HAY7369481.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /locus_tag="JN749_003155"
     CDS             7279..8223
                     /locus_tag="JN749_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HAY7369482.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="JN749_003156"
     CDS             8409..9791
                     /locus_tag="JN749_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7369483.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="JN749_003157"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="JN749_003157"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HAY7369484.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="JN749_003158"
     CDS             complement(10547..10882)
                     /locus_tag="JN749_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HAY7369485.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPIFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="JN749_003159"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="JN749_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HAY7369486.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2994 a         3012 c         2834 g         2945 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggcga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagattcgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tcttttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gcagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa agatcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaa
//
LOCUS       DADLTF010000123        11683 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.123, whole genome shotgun sequence.
ACCESSION   DADLTF010000123 DADLTF010000000
VERSION     DADLTF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.123"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(57..3065)
                     /locus_tag="JN749_003160"
     CDS             complement(57..3065)
                     /locus_tag="JN749_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7369487.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PGATTLVRLVSEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQRTSPLEQLKKG
                     PVTVSGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMASVKYIARMPQQ
                     RKLAVLTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRTLKDLDRAALIL
                     ARACSLLLDEQADDAELRETIFNSIPKSRLAESVSKVNELARPQNNNFHDEMVEQYGR
                     VKRFLPAVLRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCR
                     ALGHPTDGHKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEP
                     TSLHRLNSRVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLM
                     AEACNIGLEPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVD
                     KSANYGALDELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINM
                     LGHYTFTLPEDILKGELRELNFNLNNELTS"
     gene            3229..3786
                     /locus_tag="JN749_003161"
     CDS             3229..3786
                     /locus_tag="JN749_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7369488.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            3969..4829
                     /locus_tag="JN749_003162"
     CDS             3969..4829
                     /locus_tag="JN749_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7369489.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            5039..5578
                     /locus_tag="JN749_003163"
     CDS             5039..5578
                     /locus_tag="JN749_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7369490.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(5550..6386)
                     /locus_tag="JN749_003164"
     CDS             complement(5550..6386)
                     /locus_tag="JN749_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7369491.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(6386..7189)
                     /locus_tag="JN749_003165"
                     /note="aph(3'')-Ib"
     CDS             complement(6386..7189)
                     /locus_tag="JN749_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7369492.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(7250..8065)
                     /gene="sul2"
                     /locus_tag="JN749_003166"
     CDS             complement(7250..8065)
                     /gene="sul2"
                     /locus_tag="JN749_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7369493.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            8089..8166
                     /locus_tag="JN749_003167"
                     /pseudo
     CDS             8089..8166
                     /locus_tag="JN749_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8395..8571
                     /locus_tag="JN749_003168"
     CDS             8395..8571
                     /locus_tag="JN749_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7369494.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(8753..9757)
                     /locus_tag="JN749_003169"
     CDS             complement(8753..9757)
                     /locus_tag="JN749_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="HAY7369495.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLAT
                     CPATTIAMEACGGSHFMARKLEELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISR
                     LSTLLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCV
                     GTLTASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLL
                     VQCARVFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA
                     "
     gene            complement(9836..11602)
                     /locus_tag="JN749_003170"
                     /pseudo
     CDS             complement(9836..11602)
                     /locus_tag="JN749_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT         2662 a         3155 c         3182 g         2684 t
ORIGIN      
        1 ggtatgcctg attgttacaa gatggggttt gagggccaat ggaacgaaaa cgtacgctaa
       61 gaagttaatt cattgtttaa attgaaattt agttctctca gttccccctt caaaatatcc
      121 tccggtagcg taaacgtata atgccccagc atattgatat gcccgtgcat cagtggggac
      181 aaccgggcga tatcttcaac cccgatttct tctccattac tgcgtatcca gctcagggct
      241 tcctgcatat aaagtgtgtt ccacagtacc actgcgttag tgaccaggcc cagtgccccc
      301 agctgatctt cctgaccttc acgatagcgc tttctgatct caccgcgctg cccgtagcag
      361 atcgccctcg ccacagcatg gcggccttcc ccccggttta gctgcgtcag gatccgccga
      421 cgataatcct catcatcaat ataattaaga aggtacagcg tcttgttgac gcgccccact
      481 tccatgatcg cctgtgccag ccctgatggg cgcgagcttt tcagcaaaga gcgaatgagt
      541 tctgaagcat gaatggtgcc cagtttcagc gaaccggcgg ttcgcatcat ctcatcccac
      601 tgatcctcgg ctttcgacag atcggcacaa ccacgtgcca gttcgtccag tgcaccgtaa
      661 tttgccgatt tatccactcg ccagaatacc gcttcaccgg catcggcaag ccggggggaa
      721 aactggtatc ccagcagcca gaagaggcca aaaataatgt cgctggtacc ggctgtgtct
      781 gtcatgatct caaccggatt cagccctgtc tgctgctcca gaaggccttc cagcacaaaa
      841 atggaatctc gtaatgtgcc ggggacaacg atgccgtgga atccagagta ctgatcagag
      901 acgaagttgt accaggtgat gccacgtccg gaaccaaaat attttctgtt aggtcctgaa
      961 ttgacggttt tcaccggcgt gacaaagcgc atgccatcag ctgaagccac ttcgccacca
     1021 ccccagcggc cagcaagctc cagtgtggac tgaaaatcaa ccaggcgggc attggcgctg
     1081 accagcgttt ctgcccggag gtaattctgt tttacccagc taagccggtg gcgcgtcagc
     1141 gccggtatat tgtgctttat cagcggttcc agcccgatat tgcaggcctc tgccatcagg
     1201 accgcgcaca ggctgatgtg cagatcctgt gcccgggccc cggattcact gacatgcgta
     1261 aactcacgtg taaatcccgt tctggcatct atttcaagca acagttccgt caaatctact
     1321 ggcgggagta gcagccttac ccgactgttg agacgatgca acgatgttgg ctcctccagt
     1381 ttctccaggc tgctgatagt cagggaagga tgtttaccgt catggcaaat atgaacctcc
     1441 gcatttcctt caaagcggga tgcgacggtt ttccaggttt catctagctg gaccgccaac
     1501 tgttgcacgc ctttatgtcc atcagtggga tgtcccagcg cccggcaaac ggggacccgc
     1561 tgagcctgcc attcttcccc ctgcaacagc ttctcgcggg gatttcccca gcgatcgctg
     1621 ttttcgagcc agatgtccct tcgacgtagt gcatcctgaa ggcgctccag cagacaaagc
     1681 gagtaaccgg cacgctgtat ccggccctcc gcatcgtata ccaggcgttt ccaggggccg
     1741 gtaataatat gttcaggcgc attgtccagg atgcgctttt tcgagccgtt cagttcggtc
     1801 agataatgaa tggcggacaa cgtatgttct ccggctggcg ctgcctggaa atgcaggtcg
     1861 cgcaataccg ccggaagaaa gcgctttacc cgcccgtact gctccaccat ttcgtcatgg
     1921 aaattgttgt tctgaggccg ggccagctca tttaccttgc tgacggattc tgccagcctg
     1981 cttttcggta tgctgttgaa tatggtctcc ctcagttcag catcgtcagc ctgttcatcc
     2041 agcaacaatg aacatgcccg cgccaatatt agcgcagcac gatcaagatc tttaagcgtc
     2101 ctgagccgtt ttttctgccc cgttttcttc gccgcacgag tgatatccag tatcagcata
     2161 tcgagcacat caacggcctc atccagcgcc gcagtctcct gcgctttaac gaatgctgtg
     2221 agtacggcca gttttctctg ctgtggcatt cgcgcgatat attttaccga cgccatgcct
     2281 gcgtaacggg ccaaattacg cagttgtata gcaggcagac cggtaaaatt cagtcgggaa
     2341 aattccaggt ttcgcagccg gatataccgc tccagcgctt cggtaaatgc cgggccgctg
     2401 acggtgaccg gtcccttttt cagttgctcc agcggtgaag tacgctgccc ctcaggaatg
     2461 tccagaagct ccgtcacgcg ggcggtctgc cagctgtccg gcaacgcggc cagtttcttc
     2521 cacagccgct gatttgcccg ttcgcgtatt tcactgacga gacgtacaag tgtggttgct
     2581 ccgggcagca ggaccttatt ctgaagcagc cacgcggtgg caaaatcaaa cataaggccg
     2641 ggtcgctcat tactgagcca tgcgcgggtg tacagcaggc gcttcaggcg gaaagaccag
     2701 gggaaatcac caaattcatg gtaaccgtaa tattccttaa tcagcgcagt atgctctctg
     2761 agggtggtaa cccgttctgc gtagcgggta aggacttccg gacggcgaat attaagctgc
     2821 accgcgacaa attgctgaac acctggcaga acccgggtta agtctgtcag aaaggtgccc
     2881 agaaaacggg cggtggtgag ctgaagcgca attcccagcc ggttatgcct tccccgccgc
     2941 tggttaatga aggcaagatc ccgctcatca agatgaaaat agcgcgccag ctgcacgtca
     3001 ttgggttctg caacatagcg accataattc agttcctgct cagtggtcag aaaatcgacg
     3061 ggcataccgg cctccctgcc tgatggtcat gaattaacaa tttcgcaacc gtccgaaata
     3121 ttataaatta tcggacatcg taaaactgtt acattaatat gtctattaaa tcgtaaattt
     3181 gtaataatag acatgagttg tccgatattc gatttaaggt acatttttat gcgacttttt
     3241 ggttacgctc gggtctcaac cagtcagcag tctcttgatc ttcaggtcag agcactcaaa
     3301 gacgcaggtg tgaaagcaaa ccgtatattt accgataagg catccggcag ttcaacagac
     3361 cgggaagggc tggatttgct gaggatgaag gtggaggaag gtgatgtcat tctggttaag
     3421 aagctcgacc gtcttggccg cgacactgcc gatatgatcc aactgataaa ggaatttgac
     3481 gctcagggcg tggcagtccg gttcattgat gacgggatca gtaccgacgg tgatatgggg
     3541 caaatggtgg tcaccatcct gtcggctgtg gcacaggctg aacgccggag gatcctagaa
     3601 cgcacgaatg agggccgaca ggaagcaaag ctgaaaggaa tcaaatttgg ccgcaggcgt
     3661 accgtggaca ggaacgtcgt gctgacgctt catcagaagg gcactggtgc aacggaaatt
     3721 gctcatcagc tcagtattgc ccgctccacg gtttataaaa ttcttgaaga cgaaagggcc
     3781 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3841 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3901 caaatatgta tccgctcatg agacaataac cctggtaaat gcttcaataa tattgaaaaa
     3961 ggaagagtat gagtattcaa cattttcgtg tcgcccttat tccctttttt gcggcatttt
     4021 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4081 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4141 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggtgcgg
     4201 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4261 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4321 gagaattatg cagtgctgcc ataaccatga gtgataacac tgctgccaac ttacttctga
     4381 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4441 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4501 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4561 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4621 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4681 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4741 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4801 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4861 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4921 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gacccctaaa
     4981 agaactttcc gctaagcgat agactgtatg taaacacagt attgcaagga cgcggaacat
     5041 gcctcatgtg gcggccagga cggccagccg ggatcgggat actggtcgtt accagagcca
     5101 ccgacccgag caaacccttc tctatcagat cgttgacgag tattacccgg cattcgctgc
     5161 gcttatggca gagcagggaa aggaattgcc gggctatgtg caacgggaat ttgaagaatt
     5221 tctccaatgc gggcggctgg agcatggctt tctacgggtt cgctgcgagt cttgccacgc
     5281 cgagcacctg gtcgctttca gctgtaagcg tcgcggtttc tgcccgagct gtggggcgcg
     5341 gcggatggcc gaaagtgccg ccttgctggt tgatgaagta ctgcctgaac aacccatgcg
     5401 tcagtgggtg ttgagcttcc cgtttcagct gcgtttcctg tttggggtcg tttgcgggaa
     5461 ggggcggaat cctacgctaa ggctttggcc agcgatattc tccggtgaga ttgatgtgtt
     5521 cccaggggat aggagaagtc gcttgatatc tagtatgacg tctgtcgcac ctgcttgatc
     5581 gcggccgcga tagctagatc gcgttgctcc tcttctccat ccgcgttcca agctgcggaa
     5641 aggcacccat aagcgtacgc ctggtcgagc aggcgacgcg gatcgacgtc cagcgcacga
     5701 gagaatgcgt ccgccatctg tgcaatgcgt ctaggatcga gacaaaggtc gtctctgtca
     5761 gccggatcgt agaacatatt ggcggcgcca aagcccactt caccgaccag accgacggga
     5821 tctatcacca gccagccgcg actggagaac atgatgtttt catgatgcag atcgccatgt
     5881 agcccacgca gttccgaggc attgctcatc atttgatcgg ctataatcgc cgcgtggacg
     5941 tagtcagttt gacaacctgc gttttgatca tcgcgcgccc gctgaaacaa agctgcaaag
     6001 cgatcccgga tcgggagaag ggcagaaggc aggggttcct cagatgcggc atacagcttc
     6061 gccattagtt ccgctgcaat ttcggtcgcc tggtagtcgc cgtgctcggc aacgatgtga
     6121 gagagcattc gctccccggc atattcgagc aacatcagat tgttctcacg accgagcaac
     6181 cggactgctc ccctcccatt gcgccatacc agatagtcgg ccccgcgcag ttcatcagca
     6241 atgtcttcta taggtttcaa tcccttgacg attgcaggag tcccgtctgg caatgaaact
     6301 ttccaaacga ggctggaaaa ggtgtccgca atgagaacag gttgcgaaac gtgccaatga
     6361 gcaggaaaaa caggcggcat gaacatcaac cccaagtcag agggtccaat cgcagataga
     6421 aggcaaggcg ttcgcggtcg ggggcttcga tccccaatac attgaatagg acagcgaagg
     6481 cgcgctctgc ttcatctggc gctgcccagt tctcttcggc gttagcaatc atgagtgcca
     6541 aatcggcata gcgatctgct gttccgagcc gcccaaggtc gatcagaccc gtgcattgaa
     6601 gagttttagg gtccaccatg aagttcggca tgcagggatc accatggcaa acaaccatat
     6661 cggtgcgctc ttggtcgagc cgcaccggta gctctcgttc gacacgagcc aaaagatcga
     6721 gctgcggcgt actcttgtcc tcgtccggta agaagtcggg attgacggca ttgcgggaca
     6781 ccacatcaac ggcgcgtccg aacattcgcg acagcctgcg ctcaaacgga cattgatcaa
     6841 ccgataggct gtgaacagcg ccaagttgct gccccattga cggccacgct ttgagcaaat
     6901 ccgctccaga cagatcagcc gccggtactc ccggaattgc cgttatcacc aagcatgcac
     6961 cctcctgttc ctcctgccag ttgatgacct cggggcaagc cacacctcga cctttgagcc
     7021 aaatgaggcg gtcacgctct ccagcgagct caccgcggcg ggaagcaggt gcgattttcg
     7081 cgaaggcatg cccgtcacca cgtcgaaaaa caaaatcacc agattctccg cctctgacag
     7141 gcaaccagtc agaatgcgat tcaccaaaaa aaatattagt tcgattcaat ggaggttcct
     7201 tcagttttct gatgaagcgc gaatatagag aaatatcccg aatgtgcagt taacgaattc
     7261 ttgcggtttc tttcagcgcc gccaataccg ccagcccgtc gcgcaagggg cgcggctcgt
     7321 gtgtgcggat gaagtcagct ccacctgcgg cggcggcaag ctctgcagcg agtgtcgcgg
     7381 ccccgacatc ccccggacca cggcctgtga gcgcgcgcag aaaggatttg cgcgaaacag
     7441 acagaagcac cggcaaatcg aagcgcagcc gcaattcatc gaaccgcgcc agcaccgaga
     7501 gcgaggtttc gggagcagcc cccagaaaaa accccatgcc gggatcaagg acaaggcggt
     7561 tgcgtttgat accggcaccc gtcagcgccg cgatgcgcgc gtcaaagaac gccgcaatgt
     7621 gatccatgat gtcgccagcg ggtgcctcgc gccgatctgc ctgcccgtct tgcaccgaat
     7681 gcataacgac gagtttggca gatgatttcg ccaattgcgg atagaacgca gcgtctggaa
     7741 aaccgcgaat atcattgaga taggccacac cacgcgacaa ggcataggct tgcgtcgcgg
     7801 gttgataact gtcgagcgag acgggaatgc catctgcctt gagcgcgtcc agcaccggcg
     7861 cgatacgcgc gatttctgtg tcggacgaaa caggcgcggc gtcgggattg ctggatgccg
     7921 gaccgaggtc gatcacatct gccccctcgg ccatcagctt acgcgcctgc gcaatggctg
     7981 cgtctggcgc cagataccgg cctccatcgg agaaactgtc cgaggttatg ttgacgatgc
     8041 cgaaaatgat gagcgattta ttcatggggg cttctataat aataataatc gagcatgagt
     8101 ctcatacgga tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt tgcggtgctc
     8161 cattagctgc aggagcagaa tagcatacat ctggaagcaa agccaggaaa gcggcctatg
     8221 gagctgtgcg gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc ttttctgagc
     8281 atggtatttt tcatggtatt accaattagc aggaaaataa gtcattgaat ataaaagata
     8341 aaaatgtctt gtttacaata gagtgggtat cggagacgaa gaaggacaag gtacatgctg
     8401 accgatacca agctgcgcaa tctcaagccc agggacaaac tctacaaagt gaatgaccgg
     8461 gaaggtctct atgtgggggt cgcctcagaa aacggaatct atggtcactc ccgtttttgc
     8521 aacaccgatt ttgacgacaa gttggcttgc ttgaatctat ccggcgtctg aatgggattt
     8581 tattcccgcg cctcgatgag ttccgcgcct gatgaacctc cagaaaatat acggcttcaa
     8641 tgagcctttc cgttttacag gttcctcaac aggccggtgg gccgttagta tcatcaatat
     8701 cagtattcgc aaaaccagat gaatgattgt ttaaactggt gtatttctgc ctttatgctt
     8761 cgtaagtttg ctgtcgcgcc gtcagtgccc aggctattct ggccagcttg tttgccagag
     8821 cacaggtgac gacaaagttg cttttccgac acaacaactc cctgacccag tcggccaact
     8881 tgccagactg gtgttccagt ttttgtatga ataccctggc acactgaacc aacaaagttc
     8941 ggatcttttt gttgccccgc ttgctaatcc ctaacaatgt cgtccgacct cccgtgctgt
     9001 actgtcgggg taccagccct gttgccgccg caaagtcacg gctgctggcg tactgcttcc
     9061 cgtcgccaat ctcagttgaa atagtactgg cagtcagcgt tccaacgcag ggaatactca
     9121 gcaagcgctg tccaacctca tcttcgtcca actttcgttt caactgagat tccagatctt
     9181 taatctgctc aacaagatag tgataatgct gttgtaattt cagcagtaac tggctgagat
     9241 aaagaggcaa actactgtcc tcaagaaggg tactcagtcg actaataacg gcagcacctc
     9301 gcggaacgct gataccaaat tccagcagaa aagcatgcat ctgattagtt gttttcacct
     9361 tatcctgaac cagggattca cggacacgat gcagagctcg cattgcctgc tgagattcgg
     9421 ttctgggctg cacgaaacgc atagatggac gtgatgctgc ttcacagata gcttcagcat
     9481 caacgaagtc atttttgttg cttttaacga atgggcggac aaattgcggt gatatcagct
     9541 ttggaaaatg ccctaactct tccagcttgc gtgccataaa gtgagaaccg ccacaggctt
     9601 ccatcgcgat ggttgttgcc gggcatgtcg ccagaaattc gattagcttt ggtcgggtga
     9661 attttttacg gtaaacggcc ttcccacgat gatcctgaca atgaatatgg aaagagttct
     9721 tacccagatc gataccaata agcgcaatgt tttccatgat ggttctccga atgaaagcct
     9781 gtcctcagca tagtactggg aaggagggag tgaccatctc attaaataaa gcacgctaag
     9841 ccggttgcag cggtcgtagc ggcctgaact tgcccgcgcc gatcttggcg ctgctgcgcc
     9901 agaggtaatc gccggtgagg ttgatgtgct cccaaccgag cggcgacagg tactgcaaca
     9961 gcgcgtcatc aacggcatgg ccgttgccac gcagcgcgtg cgcagcccgt tccagataga
    10021 ccgtgttcca caacacgacg gcagccgtta ccagattgag gccgctagcc cggtagcgct
    10081 gctgctcgaa actgcggtcg cggatttcac ccaggcggtt gaaaaacact gccctggcca
    10141 gcgcattgcg cgcctcgccc ttgttcaggc cggcatgcac gcggcggcgc agttccacgc
    10201 tttgcagcca gtccaggatg aacagcgtgc gctcgatgcg gcccagctcg cggagcgcca
    10261 cggccaggcc gttctggcgt gggtagctgc cgagctttcg gagcatcagg gaggccgtca
    10321 ccgtgccctg cttgatcgag gtggccagcc gcaggatttc gtcccaatgg gcgcggacgt
    10381 gcttgatgtt gagcgtgccg ccgatcatgg gtttcagcgc gtcataggcg gcgtcgccct
    10441 tcgggatgta gagcttggtg tcgcccaggt cgcggatgcg cggcgcgaag cggaagccca
    10501 ggaggtgcat cagggcgaag acgtgatcgg tgaagcccgc cgtgtcggtg taatgctcct
    10561 cgatccgcaa gtcggactcg tggtacagca ggccgtcgag cacgtaggtc gaatcgcgca
    10621 cgccgacgtt cacgaccttg gtgtgaaatg gcgcgtactg gtcagaaatg tgggtgtaga
    10681 acgtccgccc tgggctgctc ccgtatttcg ggttgatgtg gccggtgctc tcggccttgc
    10741 tgccggtgcg gaagttctgg ccgtccgacg atgaggtggt gccgtcgccc cagtgctcgg
    10801 cgaagggatg gcggaactgt gcgttgacca gatcggccag cgccgccccg taggtttcgt
    10861 cgcggatgtg ccaggcttgc agccaagcca gcttggcgta ggtcgtgccg gggcaagact
    10921 ccgccatctt ggtcaggccc aggttgatcg cgtcggcgag gatcgtggtc agcaacaggt
    10981 tcttgtcttt ggccgggtcg cccgatttca gatgcgcgaa atgccgagtg aagcccgtcc
    11041 attcgtccac ctccagcagc agttcggtga tcttgacgtg cggcaggatc attgccgtct
    11101 ggtcgatcag cgcttgggcg gtgtcgggta ccgccgcgtc gagcggcgtg atcttcaagc
    11161 ctgactcggt gatgatggcg tccggcagct cgttggccgt cgccatacgg ttgacggtgg
    11221 caagctgtgt ttccagcagc gtcagccggt cgttcaggta ccggttgcag tcggtggcca
    11281 cggccagcgg caattcgctg gcctgcttga ggctggcgaa tttcgcgggc ggcaccaggt
    11341 agtcctcgaa gtccttgaac tggcgcgacc cctgcaccca gatgtcgccg gaacgcaacg
    11401 cgttcttcat ctccgacagc gcgcacagtt cgtagtagcg ccggtcgatg ccggtgtcgg
    11461 tcatgaccag cttctgccag cgcggcttga tgaactcggt cggcgcgtcg gcgggcacct
    11521 tgcgggcgtt gtcgctgttc atgccgcgca gcacctcgat ggcgtcgagc acgtccttcg
    11581 cggcgggagc ggcccgcagc tggcactgtt gcaaagttag cgatgaggca gccttttgtc
    11641 ttattcaaag gccttacatt tcaaaaactc tgcttaccag gcg
//
LOCUS       DADLTF010000124        11662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.124, whole genome shotgun sequence.
ACCESSION   DADLTF010000124 DADLTF010000000
VERSION     DADLTF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.124"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(113..489)
                     /gene="rnpB"
                     /locus_tag="JN749_003171"
     ncRNA           complement(113..489)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="JN749_003171"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(522..1673)
                     /gene="garK"
                     /locus_tag="JN749_003172"
     CDS             complement(522..1673)
                     /gene="garK"
                     /locus_tag="JN749_003172"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HAY7369496.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHTWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(1770..2660)
                     /gene="garR"
                     /locus_tag="JN749_003173"
     CDS             complement(1770..2660)
                     /gene="garR"
                     /locus_tag="JN749_003173"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HAY7369497.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2690..3460)
                     /gene="garL"
                     /locus_tag="JN749_003174"
     CDS             complement(2690..3460)
                     /gene="garL"
                     /locus_tag="JN749_003174"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HAY7369498.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(3476..4810)
                     /locus_tag="JN749_003175"
     CDS             complement(3476..4810)
                     /locus_tag="JN749_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7369499.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5185..6756
                     /gene="garD"
                     /locus_tag="JN749_003176"
     CDS             5185..6756
                     /gene="garD"
                     /locus_tag="JN749_003176"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HAY7369500.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(6808..7632)
                     /gene="agaR"
                     /locus_tag="JN749_003177"
     CDS             complement(6808..7632)
                     /gene="agaR"
                     /locus_tag="JN749_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HAY7369501.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            7881..8681
                     /locus_tag="JN749_003178"
                     /pseudo
     CDS             7881..8681
                     /locus_tag="JN749_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="with KbaY catalyzes the formation of
                     dihydroxyacetone phosphate and
                     D-glyceraldehyde-3-phosphate from
                     tagatose-1,6-bisphosphate; subunit Z may act as a
                     chaperone for the Y subunit; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
     gene            8681..9145
                     /gene="agaV"
                     /locus_tag="JN749_003179"
     CDS             8681..9145
                     /gene="agaV"
                     /locus_tag="JN749_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HAY7369502.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9156..9935
                     /locus_tag="JN749_003180"
     CDS             9156..9935
                     /locus_tag="JN749_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HAY7369503.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            9925..10734
                     /locus_tag="JN749_003181"
     CDS             9925..10734
                     /locus_tag="JN749_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAY7369504.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            10821..11255
                     /locus_tag="JN749_003182"
     CDS             10821..11255
                     /locus_tag="JN749_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7369505.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11252..>11662
                     /locus_tag="JN749_003183"
     CDS             11252..>11662
                     /locus_tag="JN749_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAY7369506.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAH"
BASE COUNT         2783 a         3121 c         2976 g         2782 t
ORIGIN      
        1 ggtgaaactg accaataagc cgctttcttt tgggtatagc gtcgtggaca gtcattcatc
       61 tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt tttacgtaaa acaggtgaaa
      121 ctgaccgata agccgggttc tgtcgtggac agtcattcat ctaggccagc aatcgctcac
      181 tggctcaagc agcctacccg ggttcagtac gggccgtacc ttatgaaccc ctatttggcc
      241 ttgctccggg tggagtttac cgtgccacgg actgttacca gccgcgcggt gcgctcttac
      301 cgcacccttt cacccttacc tgatcccgct tgcacgggcc atcggcggtt tgctctctgt
      361 tgcactggtc gtgggtttcc cccccaggcg ttacctggca ccctgcccta tggagcccgg
      421 actttcctcc cctccgcccg tctcccccga agaggacgac gacaaagcgg cgactgtctg
      481 gtcagcttcg gcgcgcagta tagagggttt gcgcgccctt gtcaccccgt gttgcgcatt
      541 ccaatcgcca gtgtcgcggc aatattacgt gaagcacggc agatattgtc ataagccccg
      601 cggaatgctt cgtccaacgt acctatgctg gtcaatacgc tgaagaccgc atcaatgcca
      661 tgctgatgta caacgccaac atcatcggtc aggctacccg cacccgcaat gccaataacc
      721 ggtttatggt acttcttcgc cacgtttgcg acgccaatcg gtaccttgcc gtgaatactc
      781 tggctgtcaa tacgcccttc accggtgatc accagcgtac aatcgtgaat atgttcctcc
      841 agattcagcg ccgtagtgac gatttcaata ccacttttca gttccgcacc aagaaacgcc
      901 atcagcgccg cacccatacc gcccgcagct cctgcgccgg ggacatcttt tacatcaaca
      961 tgcagcgctt ttttaatgac atcggcatag tgagagaggt tattgtccag ctcaacaatc
     1021 atcgcttcac tggctccctt ttgtgggcca aagatgcgcg atgcgccgtt atcgcccacc
     1081 agcggattgg tgacatcaca agcgacgcga atgacgcaat cttttaagcg cggatcgagg
     1141 ccggaaatat caatatcatt cagagtatta agactaccgc cgccaaaacc aatttcattg
     1201 ccgttggcgt cgcataattt cgcccccagc gcctgtacca tgcctgcgcc gccatcattt
     1261 gtagcgctac cgccaatgcc gataataatg tttgtcgcgc cgctctccag cgcctgcagg
     1321 attaactcgc ctgtgccgcg tgaagtggtc acgagtggat cgcgtttttc cgcaggtacc
     1381 agctccagcc cactggccgc cgccatttca ataaacgcgg ttttgccatc gccggagatc
     1441 ccccaactgg cattcacttt ctcgcccagc ggccctgtaa cccaggtgtg acgttcagaa
     1501 ccctgggtgg ctgcaatcat cgcttccacc gttccttcgc caccgtcggc aaccggaata
     1561 gaaacgtact gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc ctgcgcaacc
     1621 tcgctggcag ataaactttc tttataagag tctggggcga ttacgatttt catacctatg
     1681 cctgttacca catgacgccg gagggcgttt ctcttattcg gcctggattc caggcccgga
     1741 ttgcaatacg ccatccgggc acgacgtcat taacgagtaa cttcgacttt cgccagtttt
     1801 tcgtagtagc acgccagggc gctatgatcc gccgttccta aaccatctgc tcgcagtgcc
     1861 tgcatcatct ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc gtgggaagta
     1921 tccagcgcat ttgccagatc cttaatatgc agatcaatac ggaagcccgg cttgaagttg
     1981 cggtccatca ccatcggcgc tttggcatcc agcacggtac tgcccgccag tccaccgcga
     2041 attgcctgat acaccaggtc cgggttaacg cccgctttag ttgccagcgt taacgcttct
     2101 gacatcgcgg caatattcag cgccacaatg acctgattcg ccagtttggt gacgttacct
     2161 gcaccaattg ccccggtatg caccacggaa cccgccattg ctttcatcaa atcatagtat
     2221 ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca gcgtaccgtc gatggctttc
     2281 ggttcaccgc cgctcaccgg agcatccagc atatcaatgc ctttcgcttt cagcgcttcg
     2341 ctgatttcac ggcttgccag cggtgcgata gaactcatat cgatcaatac cgtacctggc
     2401 ttcgcgcctt caataatgcc attctcaccc agcgccacct ctttcacatg aggggagttt
     2461 ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt agaggctgtt
     2521 tctgcacctg cagcaatcac gtcagcaata gcttctgggt tacggtcagc aaccaccagc
     2581 gagtaacctg ctttcagaag gtttttactc attggtttac ccataatccc taggccaata
     2641 aaaccaactt tcatagtcat atcaatcatc tctcttgttg cggtggtggt tattttttaa
     2701 aggtatcagc cagtttatga gtggcagagc ggaagacgcc gagatcgctg ccgacagcca
     2761 caaacgtcgc gccccattcc agataacgac gcgcatcggc ttcgaccggc gcgaggatac
     2821 cgctgggttt gccgtgcgcg ctggcacggt taaaaatgtg ctgaattgct ttttgtacat
     2881 ccgggtgtga tgcattgccg agatggccta atgccgcggc cagatcgctg gggccgacga
     2941 agatgccgtc tacgccttcg gtagcggcaa tggcatcgac gttatctacg ccctgctgac
     3001 tttctatctg gaccagaata gtgatgttct tgttcgactg agcgaaataa tccgccacgg
     3061 tgccaaacat attggcgcgg tgagaaacgg agacgccgcg aatgccttcc ggtgggtaac
     3121 gggttgatgc caccgcctgc tctgcttcct cttttgtttc tacaaaagga atcaggaagt
     3181 tatagaaacc gatatccaga agacgcttaa taattaccgg ctcgttggtc ggcactcgca
     3241 ctactggcgc gctggcgctg cctttcaagg ccattaactg cggaataaac gtggagatat
     3301 cgtttggcgc atgttcgcca tccagcacca gccagtcaaa cccagccaaa ccaagaactt
     3361 cagtgctaat cgggttagag agtgctgacc agcaaccaat ttgtacctgt ttcgcagcca
     3421 gtgcggcttt gaatttattc gggaaaacat cgttattcat cgcttatacc tttgcttatt
     3481 tctgcaattc catacgttta atgtcgccaa ctacgaagag gtagcagacc atcgccatca
     3541 gcgctgaaca tcccacgaaa accagtgctg cattgaagga gtgcagttca cttaccaggt
     3601 agccaatcac cagtggagtg acaatggagg caacattgcc aaagacgtta aagacgccgc
     3661 cacagaggcc aacaatctct ttcggcgcgg tgtcagaaat caccggccag cccagagcac
     3721 caaatccttt gccaaaaaaa gccagcgcca tcagcatgac caccagcgtg gtgttgttgg
     3781 tgtagttaca taagatgatg gtggaagcca gcaacattcc cagcacaatc ggtagcttac
     3841 gtgccagggt cagggataaa ccgcgtttga tcagataatc cgagaagaca cctcccagca
     3901 cgccgcccgc aaaaccacac agtgccggaa tcgaggcgac cagacccact ttcagaatcg
     3961 acatgccttt ttcctgcacc agataaatcg ggaaccaggt gaggaagaac caggtgatgg
     4021 tgttgataaa atattgtccg aaaaatacgc ccaacatcat gcggttagag agcaattgct
     4081 tgatgtaatg cagtttggga ccgcttgctg ccgcgctgcc cggctttttg tggtccatat
     4141 cgaccaccgc gccattttca gagataaact tcagctcttc ctcagacata cgtgggtgat
     4201 ctgtcgggtt atgaatcaac ttgatccaca gcgccgtcag cacaaaacca atcaccccca
     4261 taacggtaaa gacgtgctcc catccccagg cgaaagtcag ccagccaagc agaggcgaaa
     4321 agagcgccag cgagaaatat tgtgccgagt taaagatggc ggaggcagta ccacgttctt
     4381 tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg gaatgatgga gcttccgaga
     4441 atcccagcat aaagcgcata aagaacatgg agatccctgc ccaggccagc gggaacatat
     4501 caacaaagcc ttgcaggaag gtgaacagcg accagaaaaa gaggctgtag gtgtaaactt
     4561 ttttcgagcc aaacttatca agcaaccagc cgccggggat ttgcatcagc aagtaggccc
     4621 agccaaaagc ggagaagatg taacccatcg aaaccgcact taactgcaac tcttttgcca
     4681 cttcggtacc agcaatagac agcgttgcac gatcggcgta gttaacggcg gtaacaataa
     4741 aaataatcag taatattaaa tagcgggtat gcacaccttt ccttttttcg tcaacggtgt
     4801 ccagaatcat tttatttacc tcgggtactt atgctgattt ttattattat ggggaaggtg
     4861 ttatttataa gtttcattta tgccgtaacg acaatgaact cgggaattag tataagcagc
     4921 gcgagaataa taatcattgt gcaaatgcta atttaattaa tactttttaa atattatttt
     4981 gagcatatgc acataaggtt gcgcgctaaa gcacagattt gcgctttacc ttgccgggcg
     5041 gcactgcaat ccctgaaatg attgacattg atcacatttc tgcgtttaaa ctcctgacat
     5101 tcttatttca cccaatgaag tcatttattt ttaaatgaga ccaggtcctc attttaataa
     5161 cccctggctg gagaatattg cacaatggcc aacatcgaaa tcagacaaga aacgccaact
     5221 gcgttttata taaaagttca cgacacagat aatgtggcaa ttattgttaa tgataatggc
     5281 ctgaaagcag gaacgcgttt tccggatggg ctggaattaa ttgaacatat tccccagggg
     5341 cataaagtcg cattgctgga cattccggct aatggtgaaa ttattcgtta tggcgaagtg
     5401 attggttacg ccgtgcgtgc aatcccacgc ggaagctgga tcgacgaatc aatggttgta
     5461 ctaccggaag cgccgccgtt acacacgctg ccactggcaa ccaaagtccc ggaaccctta
     5521 ccgccgctgg aaggatacac ctttgagggc tatcgcaatg ccgatggcag cgtgggcacc
     5581 aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg caggcgtggt ggactatgta
     5641 gtaaaaatca ttgaacgcga tctgctaccg aaatacccga acgtcgatgg cgtggtgggg
     5701 ctgaatcatt tgtacggttg tggcgtggcg attaacgcac cggcggcagt tgtacctatt
     5761 cgtaccattc acaatatttc gctgaaccct aactttggcg gcgaagtaat ggtgattggc
     5821 ctgggttgtg aaaagttgca gcctgagcgc ctgctgactg gaacggatga tgtgcaagct
     5881 attccagtag aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt cggttttcag
     5941 tccatggtcg aggatatttt gcaggtcgcc gaacgccatc tacaaaaact gaatcaacgt
     6001 cagcgagaaa cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg tggcagcgat
     6061 gcgttttctg gcgtaacggc aaacccggcg gttggctatg cgtctgatct actggtgcgc
     6121 tgcggcgcaa cggtgatgtt ttcagaagtc acggaagtgc gtgacgcgat ccatctgctg
     6181 acaccacgcg cagtgaacga ggaggtcggc aaacggctgc tggaggagat ggagtggtac
     6241 gataactatc tcaatatggg aaaaaccgac cgcagcgcca acccttcgcc gggcaacaag
     6301 aaaggcggtc tggcaaacgt ggtagagaaa gcgctcggct ccattgctaa atcgggcaaa
     6361 agtgcaattg ttgaagtgct gtcgcccggt caacgcccga ctaaacgcgg attaatttac
     6421 gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac aggtggcttc gggtatcacc
     6481 gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt acccgtcatt
     6541 aaaatggcaa cccgcaccga gctggcgaac cgctggtttg atttaatgga tattaatgcg
     6601 ggcaccatcg ctaccggcga agaaactatt gaagaggtgg gctggaagtt gttccacttt
     6661 attctcgacg tcgccagcgg gaagaagaaa accttctcgg atcaatgggg actgcataac
     6721 cagctggcgg tgtttaaccc agcaccggtg acctgataat tcaattcgta ttatgcccga
     6781 tagcacacgt ttatcgggca tattgagtca cagcgaagac gcgtgatccc cgaccagaat
     6841 cacttcaacc ccagcctttc gcagtccttc cagactatcc gcaggaatgc cttcatcaac
     6901 aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactggaac gattgaattt
     6961 actggaatcg gtgaccacga tgatccgttc cgcaacttcg cacatccgac ggtttaaacg
     7021 ggcttcatct tcattatgtg tgctgacgcc gcgctccaga tcgatcgcat ctacaccaag
     7081 aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcgc cgtaaaaaga
     7141 ttgcgactga cggcgcaaat gcccgccggt catcagcagc tcaacgcctt ccgcttccag
     7201 caacgcatta gccacgttca taccgttggt catcgcaatt acgtcagtgt acttgcgcat
     7261 cagacgagca atctcaaaag tggtggtccc ggaatcgagg atcacccgat gacctggctg
     7321 aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca gtgcgctttt
     7381 atcttccact gatggctcga ctgacggcgt cgtgctatcg cagatcaacg cgccaccata
     7441 ggcacgcaca gcgatcccct gcttttccag aaaagccaga tcattgcgga tcgtcacagt
     7501 agatacgcca tacaatgccg acagatcgtt aacctgcaca ctcccttgct gtcgcagacg
     7561 ctgaatgatc tgttctcgtc gctcgctggt gcctgtcact cgcttctcac ctgaagcgtc
     7621 ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt tgcctattaa
     7681 cgcctttcta ttaagcaaat gcaagcccat cttgcccatt ggcgcaagct actctcgttt
     7741 cagtgacttt cattatgttt cttttgtgaa tcagatcaga aaactattat atttcgtttt
     7801 atttttatct caccatgatg cagtatcaat tgaaacaaaa cgaaagatta atatcgcagt
     7861 aatctgaact ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag cacaaagcgg
     7921 gcaaaacaaa tgcaatttat gccgtttgtt ccgcacatcc gctggtgctg gaggctgcaa
     7981 tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc aatcaggtag
     8041 accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt gtttgtcagc
     8101 tcgccgactc gttgaatttc ccgcaggatg cgctgattct gggtggtgac catctggggc
     8161 caaaccgctg gcaaaacctg ccagccgctc aggcaatggc caatgccgat gatttgatta
     8221 aaagctacgt tgcggcagga ttcaaaaaaa tccaccttga ttgcagcatg tcctgtcagg
     8281 acgatccaat tcccttaact gatgacatcg tggctgaacg cgccgcccgt ctggcgaaag
     8341 tggcggaaga aacctgtcgc gaacactttg gcgaagccga tctggagtat gtcattggta
     8401 ccgaagtgcc ggtacctggc ggcgcgcatg aaaccttaag cgagctggcg gtcaccacgc
     8461 cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ttttgaaaag caaggtttga
     8521 atgccatctg gccacgcatc attgccctgg tggttcaacc cggcgtcgaa ttcgatcaca
     8581 ccaacgttat tgattatcag cccgccaaag cgagcgcctt aagccagatg gtcgaaaact
     8641 acgaaacgct gattttcgaa gcgcactcta ccgattatca attgttttaa gccggattga
     8701 tgaacgcttg attcacggtc aggtcggcgt tcagtgggtc ggatttgcgg gggcaaatct
     8761 ggtgctggta gccaacgatg aggttgccga agatccggta caacaaaacc tgatggaaat
     8821 ggtactggca gaagggatcg ccgtacgttt ctggacgctg caaaaagtta tcgacaacat
     8881 tcatcgcgcc gccgatcgac agaaaatcct gctggtttgt aaaacacccg ccgatttcct
     8941 gacgctggtg aaaggtggcg ttccggtgaa tcgcattaac gttggcaata tgcactacgc
     9001 caatggcaaa caacaaatcg ccaaaacggt ttctgtggat gcgggcgata tcgcagcatt
     9061 taacgacctg aaagccgctg gggtggaatg cttcgttcag ggcgtcccga cagagcctgc
     9121 tgtggacctc tttaaattac tttgagggat tcatcatgga aatcagcctg ttgcaggcat
     9181 ttgcgttggg cattatcgcc tttatcgctg gcctagatat gtttaacggc ttaacccata
     9241 tgcaccgccc ggtggtcctc ggcccgttgg tcgggctggt acttggcgat ctgcataccg
     9301 gaattttaac cggcggtacg ctggaactag tgtggatggg gctggccccg ctggcgggcg
     9361 cacagccgcc taacgtgatt atcggtacta tcgtcggcac ggcgtttgcc attactactg
     9421 gcgtgaaacc cgatgtcgcg gtcggtgtcg ccgtaccttt cgctgtcgca gtacagatgg
     9481 ggattacctt cctgttctcg gtgatgtctg gcgtgatgtc tcgctgcgac cggatggcgg
     9541 agaacgccga tactcgcggc attgaacgcg tgaactatct ggcactgctg gcactcggca
     9601 ctttctattt tctctgcgct ttcctgccta tctacttcgg cacggaacat gcaaaaacca
     9661 tcattgatgt cctgccgcaa cgattaatcg acggcctcgg cgtcgcgggc ggcatcatgc
     9721 cagcaatcgg ttttgccgtg ctgctgaaaa tcatgatgaa aaacgtctac ataccctact
     9781 tcatcctggg ctttgttgcc gcagcctggc tcaagttacc ggtgctggct attgctgccg
     9841 ccgcgctggc gatggcgctg atcgatctgc tgcgtaaatc tcctgaaccg actcaacctg
     9901 cggcacagaa agaggaattt gaagatggca tctaatcaaa ccaccctgcc gaacgtcagc
     9961 gaaaatgaag aaacactgct gactggcgtc aatgaaaacg tgtatgaaga tcggagcatt
    10021 gccgcggagc tgacgaaaaa agatatcaac cgcgtcgcct ggcgttccat gctgttacaa
    10081 gcgtctttta actacgaacg tatgcaggct tctggctggt tgtacggtct gctgcctgct
    10141 ctgaaaaaga tccacactaa taaacgcgac ctggcgcgcg ccatgaaggg gcatatgggt
    10201 ttcttcaata cccatccgtt tctggtgacg tttgttatcg gcattatcct tgcgatggag
    10261 cgttctaagc aggacgttaa cagtattcag agcaccaaaa ttgccgtcgg tgcgccgctt
    10321 ggcgggattg gcgatgcgat gttctggcta acgctactgc cgatttgtgg cgggataggc
    10381 gcaagcctcg cgctacaagg ctctattctt ggcgcagtcg tctttattgt gctgttcaac
    10441 gtggtacact tggggctgcg ttttggtctg gcgcattatg cttaccgcat gggcgtggcg
    10501 gcgattcccc ttattaaagc gaataccaaa aaagtcggcc atgctgcgtc aatcgttgga
    10561 atgacggtaa tcggcgcact ggtggcaacc tatgttcgtt taagcaccac gctggaaatc
    10621 acggcgggcg acgcagtggt taagttacag gctgatgtta tcgacaaact gatgcctgcc
    10681 ttcttaccgc tggtctacac cctgaccatg ttctggctgg tacgccgcgg ctagagtccg
    10741 ctgcgcctga ttgcggttac cgtggttcta gggatcgtcg gtaaattctg ccatttcctt
    10801 taaatacaat gaggtattag atgttaagta ttattttgac agggcatggc gggtttgcca
    10861 gtggcatgga aaaagcaatg aagcaaatcc tcggcgatca atcgcagttt attgccatcg
    10921 attttcccga aacctcaagc gccgcgctgc ttacatcgca gcttgaagag gccatcgccc
    10981 aactggattg tgaagacggc attgtttttc tgacggattt gctgggcggc acgccgtttc
    11041 gcgtagcttc aacgctggcg atgcaaaaac cgggctgtga agtgattacc ggcaccaatt
    11101 tgcaactgct actggagatg gtgctggagc gcgaagggtt aagcggtgaa gaattccgtg
    11161 tacaggcgct ggagtgtgga caccgagggc tgaccagtct ggtggacgag ttaggccgct
    11221 gccatgaaga atatccggtc gaggaaggaa tatgacacac gttctgcgcg ccagaaggct
    11281 gctgactgaa gagggatggt tcgatgacca tcagttgcgt gttgctgacg gtgtcatcgc
    11341 agcaatcgaa ccgattccag tgggcgtgac tgaacgcgat gcggaactgc tctgccccgc
    11401 ttacatcgac acccatgtac acggtggtgc gggcgttgat gtaatggatg acgcgccgga
    11461 tgtactcgac aagctggcaa tgcacaaggc acgcgaaggt gtcggcagtt ggttaccgac
    11521 caccgtaacc gcgccgctta ataccattca tgcggcgctg aaacgtattg ctcaacgttg
    11581 ccaacgcggc ggacctggtg cgcaagtgct ggggagttat ctcgaaggac cgtacttcac
    11641 gccgcagaat aaaggcgcgc at
//
LOCUS       DADLTF010000125        11650 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.125, whole genome shotgun sequence.
ACCESSION   DADLTF010000125 DADLTF010000000
VERSION     DADLTF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.125"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="JN749_003184"
     CDS             complement(34..1398)
                     /gene="tdcG"
                     /locus_tag="JN749_003184"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7369507.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALHRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1470..1859)
                     /locus_tag="JN749_003185"
     CDS             complement(1470..1859)
                     /locus_tag="JN749_003185"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HAY7369508.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(1873..3980)
                     /gene="tdcE"
                     /locus_tag="JN749_003186"
                     /pseudo
     CDS             complement(1873..3980)
                     /gene="tdcE"
                     /locus_tag="JN749_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(3990..4166)
                     /locus_tag="JN749_003187"
     CDS             complement(3990..4166)
                     /locus_tag="JN749_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate acetyltransferase"
                     /protein_id="HAY7369509.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTEL"
     gene            complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="JN749_003188"
     CDS             complement(4200..5408)
                     /gene="tdcD"
                     /locus_tag="JN749_003188"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HAY7369510.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="JN749_003189"
     CDS             complement(5434..6765)
                     /gene="tdcC"
                     /locus_tag="JN749_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HAY7369511.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMAGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="JN749_003190"
     CDS             complement(6787..7776)
                     /gene="tdcB"
                     /locus_tag="JN749_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HAY7369512.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAILGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="JN749_003191"
     CDS             complement(7875..8813)
                     /gene="tdcA"
                     /locus_tag="JN749_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HAY7369513.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            9003..9347
                     /gene="tdcR"
                     /locus_tag="JN749_003192"
     CDS             9003..9347
                     /gene="tdcR"
                     /locus_tag="JN749_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HAY7369514.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            9603..10142
                     /locus_tag="JN749_003193"
     CDS             9603..10142
                     /locus_tag="JN749_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369515.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            10164..11340
                     /locus_tag="JN749_003194"
                     /pseudo
     CDS             10164..11340
                     /locus_tag="JN749_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
BASE COUNT         3291 a         2823 c         2530 g         3006 t
ORIGIN      
        1 ttgtgcaccc aaggatgaaa gctgacagca atgtcagccg cagaccactt taatggccag
       61 tcctccgcgt gatgtttcgc ggtatttatc gttcatatct ttgccggttt catacatcgt
      121 ctcgatcact ttatcgagtg aaacacgcgg tgccgaggtg cggcgcatcg ccatccgcgc
      181 ggcgtttact gctttcacgg cattaatggc attacgttca atgcacggga tttgtacctg
      241 tccggcaacc ggatcgcagg tcagcccaag gttatgctcc atcgcgattt ccgccgcatt
      301 gcatacctgc gccggactgc cgcccagtag ttcagttaac cctgccgctg ccattgaaca
      361 ggccacgcca atctccccct gacagccgac ctccgcgcca gagatggagg cgttcatttt
      421 atacagcgcg ccaatagccc ccgctgccag aaaatagcgg gcaattgacc gctcgtttac
      481 cggacgacgg aacttatcgt aataagccag cactgccgga ataatgccgc acgcaccgtt
      541 agtcggtgcc gttaccacgc gcccgccagc tgcgttttct tcactaaccg ccagcgcgta
      601 catgttgatc cagtcgatga cattcatcgg atcgttagag atgttatcgc tagaaaccag
      661 ctgacgatgc agcgctacgg cacgacgcgg cacattgagc ggaccaggca gcacgccttc
      721 agtgttcatc ccgcgttcaa taccgtcatg catcacttgc cagatacggg caaaccctgc
      781 gtcaatttcc gctttgctgc gcagcgccag ctcgttgtgc atcatcaggc cagatataga
      841 caggccgttg taatcacaca ttttcagcag ttcacctgct gagtggaaat cgtaaggtac
      901 ggacgtttcg acatcgtgcg acaggccgaa gtgttcttct tcgacaataa acccgccgcc
      961 gacagagtaa taggttttac ttaatagcgc ttcctgtccc ttccaggcag tgatccgcat
     1021 tccgttctca tggcgaggca acatttcggg atggaagata atgttctttg ctacaggaaa
     1081 atcaacaata tgcgcaccag atgccactgg cagccgcccg ctgcgcgtta ctaactctat
     1141 aaatgcaggg atctcatcaa tgacaacatc ctgcggactg tttcctgcca gtcccatgat
     1201 aatggcgaca tccgtggcat ggcctttgcc cgtcagtgac aacgacccgt acagatcgac
     1261 cacaatatgg ctcgtcgcgg ttaataagcc gctactttcc agccgatcaa taaaactttt
     1321 tccggcattc attggcccca cggtatgcga actggaggga ccaatcccaa ttttgaaaat
     1381 atcgaatgca ctaatcatat ccacaccctc ggattgccgt tcagtgaagt ggagcggaac
     1441 gaccttgcga ccgtcccgct cacgaggctt tacgcactac gtactgcgat agcttcaatt
     1501 tccagcttca catctttcgg caaacgcgca acctgcacac agctccgggt cgggtaggtc
     1561 gcctgatgct catcgaagaa ctgcttatag acttcattga tggtggcaaa atcattcaga
     1621 tcggtgatga acacggtcat cttgatgata tcgcccacgc tcagcccggc agcaaccacg
     1681 atcgctttga cgttttcgag gcttaaacgc gcctgatctt gcacatcagc cgggatctca
     1741 ccggtctgcg ggcaaaccgg aatttgcccg gaggtgaaga ccatgctgcc taaatcaacg
     1801 ccctgaacat aagggccgat tgcgcctagg gcacgttgcg tttcgataat ctttttcata
     1861 catcctccgg cgtcagagcg cctgggtaaa agtacgtgaa ataacatcct gttgctgttc
     1921 acgggtcagt gcgttgaagc gcacggcgta gccagagaca cggattgtca ggttaggata
     1981 tttttccggg tgctcgatgg catccagcag catttcccga ttcattacgt tgacgttgag
     2041 gtgttgaccg ccttcgacat ccgcttcgtg gtggaaatac ccatccagca ggccgacaag
     2101 gttggtttta cgtactggat cttctttgcc cagcgccgca ggaacgattg agaaggtgta
     2161 cgagatccca tctttggcgt aggtgaacgg cagtttcgcc accgacgtca atgaggccac
     2221 ggcacctttg cggtcacgac catgcatcgg gttagcgccc ggcgcgaacg gtgttccggc
     2281 gcgacgaccg tccggcgtat taccggtttt ctggccgtac accacgttag aagtgatagt
     2341 cagaatcgac tgggtaggga cggcgttgcg ataggttggc agcgctttaa ttttcttcat
     2401 aaagcgttca accaggtcgc aggcaatgct gtctacgcgc tcgtcgttgt tgccgtactg
     2461 cggatattca ccgtcgattt caaagtccac cgccaggccg ttttcgtcac ggattggttt
     2521 cacgcgggca tatttgatgg cagacaggga gtccgtcgcc accgacaggc ccgcgatgcc
     2581 gcatgccata gtgcgataga catcacgatc gtgcagcgcc atcagcgaag cttcgtagct
     2641 gtacttgtcg tgcatgtagt gaatgatatt cagcgcgctg atgtactgca ccgccagcca
     2701 gtccatgaag tgatcgaggc tgtccatcac tttgtcgtaa tccagcacgt cgtccatcag
     2761 cggtgctgtt ttcggcccga cctgaatctt cagcttctcg tccaccccgc cgttaattgc
     2821 gtagagcagc gttttcgcca ggttagcgcg tgcaccaaag aactgcattt gcttaccaat
     2881 caccattggg ctgacgcagc aggcaatcgc gtaatcgtcg ctgttgaagt cagtacgcat
     2941 cagatcgtca ttttcatact gcaaggaaga ggtgacgatc gacacctgcg cggcatattt
     3001 tttgaaggca atcggtaatt cttccgacca aagaatggtc aggttaggtt ccggtgccgg
     3061 ccccatagtg tgcaggtgtg caaatagcgg aaggagtttt tggtcaccag cgtacgaccg
     3121 tccagcccca tcccgccgat cacttccgtc gcccagattg ggtcgccgga gaacagcgaa
     3181 tcaaattccg gtgtacgcag gaagcgtacc atacggatct tcatgatgaa gtgatcgatc
     3241 agttcctgtg cctgctgctc attgaggacg ccagctttaa agtcgcgctc aatgtagata
     3301 tccaggaacg atgccgtgcg ccccagcgac atcgcgccgc cattttgcga tttcactgcc
     3361 gccagataag cgaagtagag ccactgcacc gcttcctgtg cattctgcgc cgggcgagag
     3421 atatcaaagc catatttcgc cgccatttcc tgaatctgca acagcgcatg acgatgctct
     3481 gccagctcct cacgcagacg gatggtggct tccagatcct cgcctttttc cagacgagac
     3541 tggagatcgg caaattgcag ttcgcgttca cgcaccagat aactgatgcc atacagcgct
     3601 acgcggcgat agtcaccgat aatgcgccca cgaccatagc catctggtaa accggtcagc
     3661 acgccagatt tacggcagcg cagcatatcc ggtgagtaaa catcaaatac gccctggtta
     3721 tgggttttac gcagatcggt aaacagatat tcaaattcac tgtccatttc acggccatag
     3781 gcgtggaatg aacttttaat catattaatg ccaccgaacg ggtgtagcgc acgtttcaac
     3841 ggcgcatccg tttgcaggcc aacaattttt tccagcggct ggttaatata tcccgcatca
     3901 tgagcggtaa ttgtggtggc aatattggta tcgaaatcaa ccggcgcgtg ggttgcgttt
     3961 tcgatacgga tgccttccat tactttttct cacaattccg tggtggcagg cgtcgcttcg
     4021 gcgagaaaag attcatcgcc ttcatacggt gtatagttat gttgaataaa atcgcggaca
     4081 ttaatttcgc ttttccagtc cgtaccttta aagccaagcc atgcgtcagc gtacagctta
     4141 tcgctggtat caatatctac cttcatgaaa aataatctct ctacaatact tcaactaaat
     4201 tatgcaaatt ctgcgggcgc gttaacttta cctaaatgaa tggcatccaa agcaatcatt
     4261 ttttcttcgt tagtcggaat aacgacgcaa atgacatgcg cattttcact ggaaacaatt
     4321 cgctcgccaa aagagttaga tcgattattc atttctgtat caatctctac gcctaataca
     4381 gccaaatgtt ccatgaccag acgacgaatt aaacttgaat tctctcctat tccgccggtg
     4441 aatataattc catccagccg acgtaatgaa gctgcatgtc cggcaatatg acgggcaatt
     4501 cggtgaacaa aggttttaat tgccagttgc gcgcgttcgt gaccttcatg ccaggctttt
     4561 tccagaacac gtaaatccga agaaagaccg gaaataccta ataatcccga ctctttattc
     4621 actacgcgtt ccaggtcacc caggctctgg ttggtttggc tggcgaccca ggacatcgca
     4681 ccaaagtcga catcgccact gcgggtaccc atcatcaagc cttccagcgg cgtcattccc
     4741 attgaggtgt caacactctg accgttgcga actgcgcaga ttgacgcgcc attgccaaga
     4801 tgcgccacaa ccaggccgga gtcatcttcc gccagattca gcagcgaatg tgcgcgctgg
     4861 gaaacatagc ggtgcgacgt gccgtggaaa ccataacggc gtacacctaa ctcttcataa
     4921 tatttccacg gcaggccgta taaataagct tccggagcca tcgtctggtg gaaactggta
     4981 tcaaatacgg ccacctgagt tacgcccgga aataattgct gcgccgattc aataccactt
     5041 aaatttgcgt aattatgcag gggtgccagt ggagaaacgc gacggatatt atcaatgact
     5101 tcatcggtaa taatggcgga ctcggtaaaa atactgccgc cgtgagcgat gcggtggcca
     5161 attaaggcca cgctgtcatt taaattccgt ttttccagtt caaatgcaat tgccttcaat
     5221 gcaccttcgt agctgtggag agccagcggt gctggctctc ccccatttac ggataagaat
     5281 gcattttccg agttaatacc gtcggcaata cctgacatta atacttcaca gtcgctggca
     5341 tcgagtacgg aaaacttaat cgaagacgaa ccacagttaa taaccaaaac aaccggaaat
     5401 tcattcatct cttttctcat cctgagttac ggattaaaac agtttgtata cgatgttcag
     5461 gatggtcagc agaccaatca cggtaacaaa cacgttatcc agacgaccac ggtatttcgc
     5521 cagagacggc gctttacgga tagcatacat cggcaacagg cacagcaggg atgcgataat
     5581 cggtgcgccc atggcttcaa tcaggtcgag gatgttcggg ttggcgtagg caacaaccca
     5641 ggtggagccc atgatgaaga tcatgctgag agtattcagt ttacccagcg acactttggt
     5701 tttgtcacct ttataaccga acttcagaat cagaccattc aagccttcca gcgtccccag
     5761 atagtgaccg aagaaagatt tgaagatagc cacgagtgcg atgatggaag ccgcatattc
     5821 cagtgtaatc gcgaacgttg ttttcgtacc ggccatggac gcaaagtggt tagccagata
     5881 agaaagcact ggaatattct gcactttggc ttccgccatg ttggccggag acagagtaaa
     5941 caggcagcta aaggcaaaga acatcaccac tgcaaccatc agcatgctgg cacgagaaat
     6001 gatttgggaa catttacgtt cggtgaagtc gcgaccgaag tctttctcat actcttcacg
     6061 tttagaaacc acgaaggaag agacgattgg cgagaagtta aaggagaaaa ccatgatgga
     6121 aatccccagc cagacagtga tcaggatacc gtcatgaccg gttaacgaca gcgaaccgag
     6181 gtcaacctgg tcgataactg cagagttcca gtaagggatc agcgacagag aaatcagcac
     6241 caggctggcg ataaacggcc ataccaggta gctcatcact ttaaccatca gatccttacc
     6301 aaaccagatg acgaaagcca tcagcagcaa caggaacagc gccacaaagc cgcgattcag
     6361 cggtgcaaat ccgagctggt tttcccagaa cgtcataaag gtattggtaa tagtaacgcc
     6421 ataaatccac agcagtgggc aaatcgcgaa gaagtacagg aacgtgataa ccacgccgcc
     6481 agttttacca aaatgctctt ccaccgtttc cgtaatgttg ccggaagggt tagagccaga
     6541 aagacacaga cgcgccagcg cccggtggca ataaaatgcg atggggtatg ccaataccaa
     6601 catcagaaga atcgggatca gtccgccaaa acctgcgcgg atagggaaga acagcacccc
     6661 ggcgccgatt gccgtaccaa acaagcctaa cgtccatgtg gtatctgatt tacgccagga
     6721 cgattgtttt gtctggctgg atacaatgct atctgaagta ctcatatcct atcctcaacg
     6781 aattaattaa gcgtcaacaa aaccggtgat ttgagagacg cgagaaagat cgatattgcc
     6841 gccggaaata atactgacgg tttttctgtt ttgaatatat tggtctaatt taccgcttaa
     6901 taatgcagca catgccagag cgcctgcgcc ttcggtgacg actttattgc gctgaattaa
     6961 ggcaatcata ctgtttctga tttcgtcttc gctgaccagc acgatgtcat cgactaattc
     7021 acgaacgatt tcgtaagtta aattacccgg gcgggagaca tcacaaccat ccgccagggt
     7081 gccggtagtt cggtgcgtgg ttatttctcc ggagtggaaa gaagccgcca tgccgtgaac
     7141 gttttctgac tgtacgccaa taacacgaat ggtcgggtta atagatttaa ttgccaccgc
     7201 aataccagca attaaaccac caccaccaat tggcacaatc acgttatcga catcatagag
     7261 atcttccata atttccagac caatcgttcc ctggccagca atcactttcg gatcatcgta
     7321 aggtgggata aaaatacgac cttccatttc gacaatttcg ctcactttag cgatagtgtc
     7381 gttgaagtta tcaccatgca gaacgacttc tgcggagtag tcgcacgttg ccgctacttt
     7441 ggattttggc gcaccttttg gcatcaccac tttaccgtcg atacccagta tcgcgcagga
     7501 gagcgaaacc ccttgcgcat ggttgcccgc agaacaggcc accacgcctt tgcgtttttc
     7561 cgcatcggtc agtgaactta atttattaaa tgcgccacga attttaaatg aacccgtacg
     7621 ctgcatattt tcaaacttca ggaatatttc acctttgcaa cgttcactaa aatagttgga
     7681 gcgaggcatg cctgttttat aaattcgccc agccagtcgt tgtttcgctt caataatatc
     7741 atcaatagca accggcagat cgtatgtaat atgcattata aaacctcttc gcctgaatta
     7801 aatatgtagg taaccgtaac cgacacctgc aaaacagaca ggtggattat ttatgtttaa
     7861 ataaaaacaa ataactaacc aacttctatt aattgccttc gtctacaccc attataagat
     7921 gaatactctt tggctaattc caccaaaacc gatgctgctt ttttaatacg ataatttttc
     7981 gaccataccg cggcatattg tgccacaggt aatgtttctt caaccggaat agtaataaat
     8041 tgattagaac caaaaggtga cgtcatatca caaggaatta cagttaagaa atcagcattg
     8101 agaacaagat tataaattgt cacgactgag tcggttttaa cgatgttttc aatactgatg
     8161 ccatttcttt gtaacgtagt aagcagttcg ctgtagtacc ccatattagt ttgtggcaac
     8221 acccactgtt cgttcttcaa cgactccagc gtggtggtgc cggtgcatgt tcgggactta
     8281 ctggctacca gcacaaactc ggactcgaac agcggctcaa catgtaaatc ctgaagcttc
     8341 atttctgcac ttaacgtacc aatcgcaaaa tccagtcgac cgtcgcggat tgccggtaag
     8401 aacgaagaca gttgcgcttc atacatagaa acctgcgctt tcgggaacac ctctttgaac
     8461 ttgttgatca tccctgacat aaaagtaaaa ccaatcaatg aaggaaaacc aaatgagact
     8521 tccaccaccg cctcagaaga cataccgctt atctcattaa ccatattttt catttcacgg
     8581 gtaatggatt cggaacggga gagtaacaat tgaccggcag gtgttaatgt tacaccggta
     8641 tttttccgca ccactaattc cacaccaaaa taatcttcaa tatcgttaat gattttactg
     8701 acggccggtt gagttaaccc taattctttt gcagccgagc cgatagagcc acttctaatg
     8761 acttcctgaa agactaccag gtgctgcgtt ttcggaagaa gaatagtgct cataacgacc
     8821 tacgttaatt acctcattga cggcatgaag tgtatcaaaa tgaaataaac aggatatgtg
     8881 cgaccactca caaattaact ttcattactt tccaaggtat cgttattaca attaaatacc
     8941 tttaaatatc aacaagttaa agcataaaaa tcagcataaa ccctgatttc tatcaaaaaa
     9001 aaatgacggg gataaccata ttttatggcg ataacatcat tcgttatgtg gttaatacaa
     9061 aaaaggggct gagaccatat ttcaagcaat taccggataa ttaccaggca aaatttgagt
     9121 taaatttaat gagcaagttc tcaaatttta ttataaataa accattttca gcgataaata
     9181 ctgcggcacg tcacattttt tcacgctatt tgttggagaa caaacattta ttttatcaat
     9241 attttaaaat ttcgaataca ggtattgatc atctcgaaca attgattaac gtcaactttt
     9301 tctcttctga caggacgtca ttttgtgaat gcaatcgttt tccataaatt cttctcccct
     9361 cataggcgac gaatagcatt ttgtgttgag gatcacaaaa cgaataattg ctggtcgccg
     9421 cgataaggtc agacgaagtc aacaaggggg attttcactg tgctttacat cagcgtagga
     9481 cacagaatgt tgcttcgttt actgtaacgc cgggtaaatg agcgtttttt gatagtgcgt
     9541 aagtgtcgtg cgaggaaaat aagcatctat tattgtttgt catgacgaca aggcaggcat
     9601 caatgaaagg cttcccaatt gcacatattt ttcacccttc aatcccgcca atgcacgcag
     9661 tggttaacaa tcacaataga aatattgatt attggacggt aaaaagaaag tttgcagaaa
     9721 ttgtctccac caatgacgtt aataaaattt acagtataag taatgaactg cggagagtat
     9781 tatctgcaat tactgcattg aatttctatc aaggcgatgt tccttctgtc atgatccgaa
     9841 tccaaccgga aaatatgagt ccattcatca tagatatttc tacaggagaa catgatgatt
     9901 atatcataca aacattagat gtaggcactt ttgcaccttt tggtgaacaa tgtacttgct
     9961 cagccgtcaa taaaaaagag ctggaatgta ttaaggagac gatttctaag tattgcgcaa
    10021 agttcaccca aaaagaagcc atcttaacac cccctaggca ctttaataaa acctgcatta
    10081 cttcagattg ttggcaaatt ctcttttttt cacccgatca ttttaataat gatttttatt
    10141 gattatttat aaaggataaa ataatgttcc ctgtttcatc aattggtaat gatattagta
    10201 gtgatttagt ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga aataacctcg
    10261 aagcactagc acccggtata gaaaaactaa aacagacctc tattcaaatg gtcactttac
    10321 ttaacgcgtt acaaccaagt ggaaaatgca ttatcactgg tgattttcaa aaagaattag
    10381 cgtacttaca aaatgtaatt ctttataatg actcgtctct tcgtctggat tttttaggtt
    10441 ataacgccca aattattcaa cgatcggaca atacctgcga acttaccatt aatgaaccgt
    10501 taaaaaacca ggaaatatct acaggcaata tcaatgttaa ttgcccatta aaagatattt
    10561 acaatgaaat caggaggttg aacgtaatct ttagttgtgg gactggagat atagttgatc
    10621 tatcctctct ggacttacgt aatgtcgatt tagattatta tgatttcaca gataaacata
    10681 tggctaatac tattctaaat cattttaaat tggattcaac aaattttact aatgccaaca
    10741 tgtttcaggt taattttgtt agttcaacac aaaacgccac aatctcctgg gattatttac
    10801 taaaaataac gcctgtttta ataagcatta gcgatatgta ttctgaagaa aaaatcaagt
    10861 ttgtcgaaag ttgtttaaat gagcttggag acattaccga agaacaatta aaaattatga
    10921 gatttgcaat tataaaatct ataccaaggg caactcttac agataaatta gaaaatgaat
    10981 taacaaaaga aatatataaa agctcatcga aaatcaacaa ttgcttgaac agaattaaat
    11041 taccagagat gaaagaattc tcatcaaaaa aatatatgat tacatcgata caatcattga
    11101 agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta attatactat
    11161 ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa agacgaaaat
    11221 tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata taactcagag
    11281 aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa tgcaatatag
    11341 acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct taccctgtgc
    11401 accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg
    11461 atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg cgtgcttttg
    11521 taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc aacaatctga
    11581 aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag ccgctttctt
    11641 ttgggtatag
//
LOCUS       DADLTF010000126        11555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.126, whole genome shotgun sequence.
ACCESSION   DADLTF010000126 DADLTF010000000
VERSION     DADLTF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.126"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..336)
                     /locus_tag="JN749_003195"
                     /pseudo
     CDS             complement(1..336)
                     /locus_tag="JN749_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
     gene            complement(652..1296)
                     /locus_tag="JN749_003196"
     CDS             complement(652..1296)
                     /locus_tag="JN749_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369516.1"
                     /translation="MMKQGMANSISLTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1317..1586)
                     /locus_tag="JN749_003197"
     CDS             complement(1317..1586)
                     /locus_tag="JN749_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HAY7369517.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPEKLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1665..2303)
                     /locus_tag="JN749_003198"
     CDS             complement(1665..2303)
                     /locus_tag="JN749_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7369518.1"
                     /translation="MGDSVTPEIIGNMIRQYFSQERPEEETIQALNHLRRVLHEVSPF
                     AHEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2288..3520)
                     /locus_tag="JN749_003199"
     CDS             complement(2288..3520)
                     /locus_tag="JN749_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HAY7369519.1"
                     /translation="MSDTLLTEKILTGENVLRAAIARIEWIFETFPSVCLSFSGGKDS
                     TVLFHLVAEVARRRKRHFSVLFIDWEAQYRCTIEHIQKMREMYHDVTETFYWVALPLT
                     TVNGVSQFQPEWICWEPGVTWVRQPPEEAITDMTYFPFFRYAMTFEEFVPAFSSWFAG
                     NRCGVAVLTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKAR
                     DIWIYNARACAIYNPLYDLMYRADVPLRNMRVCEPFGPEQRKGLWLYHILEPETWARM
                     CERVSGAASGALYANESGAYFALRKRISKPAHHTWRSYAMFLLDVMPERTAEHYRNKI
                     AVYLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRH
                     YERYLQRMKERRKEWGIL"
     gene            complement(4021..6189)
                     /locus_tag="JN749_003200"
     CDS             complement(4021..6189)
                     /locus_tag="JN749_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7369520.1"
                     /translation="MIFPFGLNESQLLAVKRAFSSQISVIEGPPGTGKTQTILNIVAN
                     ILIQNKTVAILSNNNSAVSNVYEKMDKQQLGYVMARLGSTENRQQFFSTSISRSEEVL
                     PDSPSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFS
                     HKYRFSSQKTTDLMAYIHYLSDRRIGFRNRIDLLLNFMILKVKPLMIPERRLALFTSL
                     QLSYYEKNIREKQISLNEYEEVFKKSDFKILLGRLTSWSMLYLKQHLRRNVSTRSSFS
                     AETYRDEFDRFIKRFPIIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCA
                     RNVIVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHY
                     RCHPKIIQFCNKQFYDNALIPLTVDSGEASLSLVITAKGNHTRNFSNLRELESLEGHY
                     WDEESSRGYIAPYNAQVNLAEKVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQH
                     SRNIAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFE
                     SPVISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHS
                     QIALNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQA
                     ERDELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6943..7989)
                     /locus_tag="JN749_003201"
     CDS             complement(6943..7989)
                     /locus_tag="JN749_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HAY7369521.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSAGTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            8664..8855
                     /locus_tag="JN749_003202"
     CDS             8664..8855
                     /locus_tag="JN749_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HAY7369522.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMRDGYPHPCM
                     QPMYNFLGKFKPEPREETE"
     gene            complement(8908..9141)
                     /locus_tag="JN749_003203"
     CDS             complement(8908..9141)
                     /locus_tag="JN749_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369523.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9237..9860)
                     /locus_tag="JN749_003204"
     CDS             complement(9237..9860)
                     /locus_tag="JN749_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7369524.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9949..10458)
                     /locus_tag="JN749_003205"
     CDS             complement(9949..10458)
                     /locus_tag="JN749_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369525.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            10916..11374
                     /locus_tag="JN749_003206"
     CDS             10916..11374
                     /locus_tag="JN749_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369526.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT         3232 a         2874 c         2311 g         3138 t
ORIGIN      
        1 tgattctgga aaaggacttt gtggattgtt gctattgcca gtgagattga atgaagtcgt
       61 tttaactcgc tttgaatacg tcttgccccc agatttcgct gagaacgtaa ctccagaatc
      121 agagcgactt cgcaagtgcc ggttttcgtt gatggtgaac gcttggggcg acggctgtgg
      181 ctttccagac cagcgatgcc ctgagccaga tatctgcgcc accattttac gcaatgtggg
      241 ccgagaaatg ccacagcgac ggcatacaaa tcccgcatca ccggacgttt catagagttt
      301 tacccactgt agccgctgct gaatttccct gttcataatt tggcattagt tcagctcaat
      361 cccgacttaa aaagtcccta caggatgttc cggacctgag gcacagttca tatccttatc
      421 cataaaaaat ttagctatgt ctatagcatt aatcttgaca agacgacgtt agggcctggc
      481 gatgaatcat ttcccctgcc agatatcctt tggggtgcac cagaactacc gttttatatc
      541 tatataactg aaacgtccct cgccatcctt catattttga tgtagtaaca ccattcactt
      601 tcatctaccc cccgttttat taacatatag taaaaatcac ctcgccgcat tttattcttt
      661 tgaagtaaaa tcatttacca gttcacttaa agctctcttt tgttcatcat catacagaga
      721 ttgaatccgg aatttaatct cttctgcaac cagtcttgca tcgggctcat aaaatatttt
      781 tgttattcgc tgtttctgat gttcaggctg gtcttccata tttcgtaaaa ccagatgaaa
      841 taatatatca taaagtgcgc aatgccatga ataaccatca ttggttaaaa taagctccca
      901 aacccgctcc tgctcggcaa taagctgcag aacaaaggac tcaacctgtt ctgtttgttc
      961 gggggcaaca cggtatagcg gagcaatcac agcaaaagat agttcattta accaggattc
     1021 cgcttccttc tcgctcctga acggtggaca gttttgtatt cttcgttcgt attcacgggt
     1081 aatctgctcc agaatatcct ccggcggtgt cagccactta ttcctgagca atacactggt
     1141 tgatggatga cagctggcta tttcagcaat aatttcacgt aatgtttccg ggctgaactt
     1201 cttcagctga ttgtagtgct cccgggtaaa accatgttca tcgcataaaa gttgccatgt
     1261 cagtgatata ctgtttgcca ttccttgctt catcattaat acctgttacc tgaatatcaa
     1321 cagaccggtg taccttttct gaatacctcc ccggacggag tggcaatccc cattcgcctc
     1381 agcgtggcat ctgcagcttt cgccacctcg ctgatatcca gcttttcagg tagcggcggg
     1441 gacgatatct gcgccagaca ataattccgg cgcaacaaca accactcccg ttgtgcttct
     1501 gtacattcaa catttcccat aacaaaaagc cagcggtcag aagctctgcg ccagcaacca
     1561 gcagcctcca gtttctgcgc aaccatgtct tccacaactt ccggggtact ctttttcctg
     1621 ctcaaaacat taactcactg aaataatata catatctgac ggtctcagcg aaccgtccat
     1681 gccgggctga aatcctcttc ctgaaacagc tccgtcagcc cgctaatctg tttcaatctg
     1741 agaacttcat cctggtccat acccagctcc gtaccaatgc gttcattcgt ccagcccagg
     1801 cgcgacaggt ccctgacaat atcagacatt gaggttatct gatgtttgcc cctggcccgg
     1861 ttatgtcgga tggtcgcagc aatacgtgag gcctgtccct ttctctccgg attaatacag
     1921 accaccggca gccagccctt cagacgtttt cccgtacctg cttccctgcc cagcacctgg
     1981 cgatgaaaac catccaccac caggtaatgg ttcttttcct cagacaccac caccggttgt
     2041 gtgaaaccat ctttctccag tgactgcttc agcagtttct tctcacccgg cgccatgaca
     2101 ttaggattgt aatcattggc caccacctca tctgctttta cccacagcac acagtccact
     2161 ggctcatgtg caaaaggact gacttcatga agcactctgc gcaggtgatt aagggcctgt
     2221 atggtctctt cttctggtcg ttcctggctg aagtactgac gaatcatgtt gccaattatc
     2281 tccggtgtca cagaatcccc cattccttcc tcctttcttt catacgctgc agataacgtt
     2341 catagtgccg gggtttgttc ggactgaagg agagggtccg gcaccagaaa tcattcttta
     2401 taagtgtctt acagatacgc cgccaggacg gaatatcccg gctccccagg tcattctcct
     2461 gttcatccgg gatgtcatcc gggaagcccc gcgtctgata ccagcgcagg tagacagcaa
     2521 ttttattacg gtaatgttct gccgttcttt ccggcatcac atccagcagg aacatcgcat
     2581 agctgcgcca ggtatgatgg gctggttttg atatacgttt acgcagggca aaataggcac
     2641 cgctttcatt ggcataaagc gccccgctgg cagcgcctga cacccgctca cacatcctgg
     2701 cccaggtttc cggctccaga atatggtaaa gccacagccc cttacgttgt tcaggaccaa
     2761 aaggttcaca gacacgcatg ttgcgtaacg gcacgtcggc acggtacatc aggtcataca
     2821 gaggattgta aatggcacaa gctctggcgt tatatatcca gatatcgcgg gctttccagt
     2881 catacaacgg atacatggtg taataaaacc cttcaggcga tgccgtggtc cagggcttat
     2941 catcggcata tctcagtttg cgctgagaca ccagccccat aaagcgattg agggattcat
     3001 ccgcacgaac accggtcagc actgccactc cacaccggtt accggcaaac caggaagaaa
     3061 atgccggaac aaattcttca aacgtcatgg cataccggaa aaagggaaaa tatgtcatat
     3121 ccgtaatggc ctcttccggt ggctggcgaa cccaggtcac tcctggctcc cagcatatcc
     3181 actccggctg aaactgcgag acgccgttta ccgtggtcag gggaagtgcc acccagtaaa
     3241 aggtttccgt cacatcatgg tacatttccc gcatcttctg aatatgttca atggtgcacc
     3301 gatactgggc ctcccagtca atgaacagaa cagagaaatg acgttttctc ctgcgggcca
     3361 cctctgccac aagatggaaa agcacggtgg aatcctttcc acctgaaaaa gacagacata
     3421 ccgacggaaa tgtttcaaaa atccactcaa tgcgggcgat tgcggcacgc aaaacatttt
     3481 ctccggtaag gattttttcg gttaaaagcg tatcactcat tttcctggct ttatgtcgaa
     3541 taattctgtt tatacattca ggcaaacttc attaataact caccgctttg gggataaata
     3601 ataacaacaa aaatcttcag atattaacct ggttgttgga tttgttatca catcctttat
     3661 ccacctgctt cttactggta acacatctgg aaaatccatg cattttcaac atatactatc
     3721 ctttcaactt gtaaataaga aaatatgatg cagatggtga acaattcaaa tatcattttg
     3781 caggaagtat tcactgtaat agaattaccc cctgataaag tttattcact aaggaattat
     3841 tcaacagaaa cattccctgt tagctttgag caatagcact tatttttata tgtatcagga
     3901 atacgtacgt tgatgtaacc aaaacaaaga agcctgtgtc catggggttc gtacaattgt
     3961 gtgtggcaca tgaccaaatc aggtttgaga cctcccgctg caatattttt tattcagatt
     4021 ttagcctgtc aggcctgaca gaaacgctcc caacttccct tctatatcac tgtctatagt
     4081 acgtaatctt agcaatggca ggccgcattt cttcagtata ctgtttttaa gttcatcacg
     4141 ttcagcctga acactggtca gatgataacc tccatccact tcaataaccc ctaatggagt
     4201 cttcccgacc ttatagtaaa caacaaagtc acaactggcc cggttacgca tgaataactg
     4261 ttcacggtgt gtaaaatccc cccgctccag caacaccaac tgattcagcg ctatttgtga
     4321 atgaaacatc atactccgat aactatcctg cgacaggata tcccgcaaca ggcaggccac
     4381 aatctgttct gacttaaagt gtgaatcatt tgaattcaat ctactattca gtcgttccag
     4441 tgatttgtca tactcactgt acagcaaatc aaatgctgat atgaccgggc tctcgaaaat
     4501 ctctccgtca tcagcgtagt atttgatgta acgaatcagc gccgcaatat gtccagcgtg
     4561 gcgttcaaat acatcattac cagtcaccag cgtaaattta tttcgggccc ttgaaacagc
     4621 gacattcacc agttcaggat tatcaacaaa cgcgatgttc cgactgtgct ggctgctgcg
     4681 cttcttatcc agcacagtgg aaaacacaat ttcatcgcac tcccgtccct gaaatttatg
     4741 caccgtcgat ttcacaaaat cagcaggcag cactttttca gccagattaa cctgtgcatt
     4801 atatggggca atgtatcccc tgctactttc ttcatcccag taatgacctt ccagagattc
     4861 aagctccctg agatttgaaa aattacgggt atggtttcct tttgccgtga tgaccagaga
     4921 taatgaggct tcaccggaat caaccgttaa cggtatcaga gcattatcat aaaactgctt
     4981 attacaaaat tgaatgattt taggatgaca acgataatgc tctttaagaa gggttactgg
     5041 caccatgttc ctgaacaaca tacaaaccga atcaagtaga ctatatttct cacaattata
     5101 gtattctgcc ggtggagatg ggctgttcgg gagtaataca ggaacatgtg gaagttgctt
     5161 acgatcccct acaacgatta cattacgggc acatcccagt cccagaatgc ccggaacaat
     5221 atcctgctgc gaggcttcat caataataac ataatcaaga agggccccct tgccgataga
     5281 gttaataatc gaatgcgtac tgctaccaat aatcggaaaa cgctttataa aacggtcaaa
     5341 ttcatccctg tatgtctctg ccgagaatga acttcttgtt gaaacatttc ttcgcagatg
     5401 ttgctttaaa taaagcatag accatgatgt caggcgcccc agtaaaattt taaaatcaga
     5461 ttttttaaat acttcctcat attcattcag agaaatttgt ttttcccgga tatttttttc
     5521 ataataagaa agctgaagag aagtaaatag cgccagcctt ctctccggaa tcatcaatgg
     5581 tttgactttc agtatcatga agtttaacaa caaatcaatt ctgtttctga atccaatccg
     5641 cctgtcagaa aggtaatgaa tataagccat caggtcggtt gttttttgtg aggaaaatcg
     5701 gtatttgtga gaaaaaagct cctccggtcg aagattctga ctctgccatt gctgcaggta
     5761 tttatactca atattcagct cattaatttc tgcttttaac gaagcgacct ggttaatggc
     5821 attcaggtgt tttttcactt gttgaagtac atcatcaatc gcatttgcag acggtgagtc
     5881 aggtaagact tcctcagaac gcgaaatcga cgttgaaaaa aactgttgcc tgttctccgt
     5941 actccccagt cgtgccatca catatcccag ttgctgtttg tccatctttt cataaacatt
     6001 actgacagct gagttattat tggacaaaat tgccactgtt ttattctgaa ttaatatatt
     6061 agcaacaata ttgagtatag tctgtgtttt acctgttcca ggtggtcctt caataactga
     6121 aatctgtgag gaaaatgccc gtttgacagc cagcaactga ctttcattaa gaccaaacgg
     6181 aaaaatcaaa ccccatggcg aatcacgttt tttactctgt cctgaacagt atgcatgcaa
     6241 ggcggtatcc ggatgaggaa ctattttctt tatctgccta aggatattct catcgatatt
     6301 tctgttatta ccacgggcat aaaaaatacg ttcattagcc accctacaca agtaattaaa
     6361 cacggcatgc tctgtaaatg ctgaacactg ttgaaagtca aggccggtcg ttttcatgat
     6421 gtagggtcgt tcaccctcgc ggtaataaac agcagcatat ttatcaccat atataataac
     6481 cagttcagcc cgggatgtta cagcgttccc tttcctttcg aggaaaacat ttcgcaaatt
     6541 tacagttggc gttatctgac atacagaaag cggacgagta tattttctcc cgctgcgaaa
     6601 atgacaggtc agcataacct ctctgtcgtc atttccagcc cacagcgtcc agtcggtaat
     6661 tttatgagtc ttattttcac cgtcaacaca aatcgtgacc atattctcac tcagaaatta
     6721 tcatttaact tacggaaaat tgttaaggca ctcccgatat tagctccgga ggattttcca
     6781 caactccggg actgttaaca tatttctgcc tgccatatat acattgaatt tagtcataac
     6841 agtcaatacc agaattacag gaatgcgatg ccgatacgct ggttattgaa tactggtagc
     6901 ccctgagcac taattcatca cgatactctg tatttactgt tttcactgct ttttacaacc
     6961 attcccccag gtaaaatgct gctcctccgg tgatggctgt gtcagaaact gccatcccgg
     7021 aatagccacg ctgaccgcca gcaatgccgc acagacacca gccacaaacg gtttcagaaa
     7081 cgtgggcggg gatttctgaa gggagggtga ctgctccgcc tgcactttcc ccttgcgatt
     7141 atcaaggctc agtgcatcca gacgcaccgc gccatttgcc gcaagcagcc cttcccggtt
     7201 atccacgctt cccggcagac tcagcgaaaa atcccctgtt cccgtctgaa tcagactgcc
     7261 tttacgattt gacaggcttt gtgccgatat ctgcagtgtg tcggcattta tcgtaccgcc
     7321 attactgctg aactgcccgg cagtccgggc ggaaagcgta ccggcactca gttgcgcatt
     7381 ctgtgtagac agatttccac tggttgcatt cagctcagta tgttgtccct gtatccggct
     7441 gttacctaaa tccagcccct gctcaatgtc ccgtaaatcc agcagagaac cttcgtgtgc
     7501 cgggaatgag gaatacaact gaatatagtc atcactgtcc ggtgtcagac gcacatgtcg
     7561 cacaacaccg ggaataatga ccagccggag gataccgctt tttaaatcct gcgaaggtgc
     7621 cagaacacgg gtggtgacat aaccatgatc gaccagacgg ttctgcaatg tactcatcag
     7681 cagattaatt cctttcgccc ccaggcaatg cccgaccgcc ccgttggcga ttttttgtaa
     7741 aggcagccag tgtggtaacg catcagcccc cttcagttcc gtctgtttaa tctgaaaaca
     7801 gggcgtttca acaggaaaat taatcttatg ggcaaaagag ccaggtgcag aaaggcgaac
     7861 atcaggggct gaaggcgtca gttgttgctc aagggcttcc tgacgctgct gctgaatgat
     7921 aaatgcttca tctccgctac gccggacatc cgctgcctga agagataaag gcgataataa
     7981 agcagacatc agtgtcagtg acagcaagga cggacagcgc cggaataagc tttgttcctc
     8041 agtcctctcg ttcctgcaac ataattttcc agtcaaacga aaaacctttt attcatgtca
     8101 taccaacaat caccagcctc ctgacgatac ctcccaccat gttttgcgat ggcacgctac
     8161 cgcccttccg gtatttaccc gcatcaaccg gtaccagaat gctgagtaac aaaccagcca
     8221 gaattgactt aattttgatc attatagaat aatgatctga actacagcaa catgacagat
     8281 ttatcgatcg atataacaca aatgaacagt tctttattct gccatatatc gcaaaaacag
     8341 cacattacaa aatttataac cataattact ggaaaagtgc agaaaattat tccactgaat
     8401 gcttttaata cagttggtta gcttaaaaaa tagcaatttt tgatttcagt cattgaaagt
     8461 tgcaatgttt gacgattcaa cagtatttaa gtgagtttca ggatatggga aggacatgaa
     8521 aggaggagcg caataaaata cacaggaaga aggggaaaat gtttcgtaaa aacagaaaat
     8581 taacagcgca catcaggaaa cgcagtctcc tgattcagtg ctaccacatg aaaatttagt
     8641 taactgttac cgtactaaaa tttatgtcag aagatgttcc attaccgaaa gtcaatcagc
     8701 gatacaaaga cgatcatggt gcgcttgtca ccctgaccag tgttgaggaa acccgggtgg
     8761 tgtttatgcg ggacggctat ccgcatccct gcatgcaacc gatgtacaac tttctgggaa
     8821 aattcaaacc cgaaccccgg gaagaaacag aataaaacga aatccctgcc ggcaatcaca
     8881 ctacctgata gcagcataat ccacttatta attctcatcc ccggagacaa gcccagaaaa
     8941 ccactccggg tgttcactgt atgccgtgtt cagcgcatcc ctggaaacgt ttatataaaa
     9001 gctgtcctgc aatttgcggg taacaacttc tgccaggcga ttccggttat ccaccagtcc
     9061 ggcataacct ctctctgcca gccactcatt cagttcatgt tccagagaca gattgataat
     9121 atcataatcg gaccttgcca ttcccgtttc cttagcacat gttttcaggc acggggataa
     9181 agtataaaga atgtcatact gctgctgcca gagcaatatt ccccctgatg gttgtatcag
     9241 agatggtatg ccggtacttt gcgggttgtt cccgtcaggt tatcgtgcac ctccacggtc
     9301 gtggtcacca ccggcattcc ggcctttctc agcctcagaa catcagccgc aatacgctga
     9361 ctgccaaacc agaacaggcc gtccagtgca gtcaccagca aacccgcctc cagtgcatac
     9421 ttcagccgtt cacggggcgc tttcacttcc cgggcaacct gctgatatgg caatgatgtg
     9481 ttttcattcc caaccacgcg acgaatacgg tgagacagat gataccggta tgtatccggc
     9541 acaccagaaa ggctggcctt caggctgtac acgcagccaa accgtttatc gttaaacacc
     9601 acatttttct ggctgatgcc ccattcttca cgcagcgcgg caatcaattg cggtgtacgg
     9661 gtaagcagca agcgaaaagg cagttcaaaa ctggtgacgt aatccacatt cagcagtgca
     9721 atacgcaacc gttcttctgg cccggcctct gtctgccggc actcctccag aacatcctgc
     9781 cactgccggc gaagacgggg ggattcgttc cgttctgtaa aacagtattt atccgccaga
     9841 tagtcaattc gtgtacgcat actgaaagga attccgtata aagattcagc agacaaaact
     9901 ttatcagcca gtaaaaatta acggaagcat tgatattcct cccgacaatc accggatgat
     9961 tgttgcaata acctgcgaca taaaggactg aacagccgtt tttaacgcca cgtattgccc
    10021 tgatgaacca ggccgggcaa cccgaaaaca tctttccacc cggcattgtc cgccagcact
    10081 tatcaccgac caggtctgtt gcagtaaatc caccagttgc gcacattctt catcagccat
    10141 gacgccgact cgcggatggc aaatactcgt ctggctgagc agcaacagac atcgtatctg
    10201 tgctccttca tgttgttccc gcaaccagcg taataccacc ggcgctgatg ggctgaccgt
    10261 gtgattatgc tccgtaatac gttcgtgtac ccgtcggtga aaggaactac aaaatgtctg
    10321 aatctgttga ccatagattc cttctgccga atagagcgtg aagtgaaatg ccaacagatt
    10381 tggccagtgg tccaccgcct gctgaacaaa acgccggatt tcccccggct ctgaaattaa
    10441 ggcttcggtt atttgcacga aatgttcctt ttcaacccct gttgaatttt gtcaagccgg
    10501 tgcagacaca gtaaagcgcg catcaacacc tgaaaggtac gatacaaaca caagttacaa
    10561 acaataagaa cggaaggaca attaatcagt gaggaataat aactaaatta accaaaagaa
    10621 tgttactgac aatactacca atacttaaat agcaatatat taattaatgt aagaacagat
    10681 gcccggtaag acatatacac aatacatcca caaacacact cttttcatta aatcaatcac
    10741 ctgattaaac catgaaagtg tgatgtaagt caaaattact gatactatca attgataatg
    10801 attatctcta tcattatcgt gaaattcgag aatgaaagga gaagtagtca ttataagcct
    10861 tttataaatc gtcatcatca tagtaaagta gaacactttt tatatgagtt actatatgaa
    10921 aatatactgg acaagaaaac gtattcctga actttcagca cttccacctt cattaagaaa
    10981 gaaaaatttt actgacgcat ataatgcagc atcttctcat atcgaatact ggataggtgc
    11041 tggcgtctca tttatctcga tgatgatttt atccagagta tatgactttc ttttacccgc
    11101 ccaagatact ttcccgggtg atatcatcag aagtttatgt gttgtgtgtc catccatatt
    11161 aatctggttt cagttttcgg tatatgtcat gaggaaatat tacagacata ttctggtgcg
    11221 aggaaaagaa accgaaacca tttcagaaag gttgatacgt gaggctgata cacgagaata
    11281 tgaactatgg cgtccggtcc gtcgcttttt ctccattgtt ttcctcattg ttctattagg
    11341 ttgcattcat tcactgataa caactataaa ataaaagtta tggctgttct gatacaggaa
    11401 aataaacgtt cattctatgg ttacctcttc ctgttatata gaagaatttt aaacgacaag
    11461 taagggaata taaatgaaaa taacaaaaaa taaaaatgca actataaaaa acagtcatca
    11521 gtcggctgct tttcaatgtt taccttatca ttttc
//
LOCUS       DADLTF010000127        11498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.127, whole genome shotgun sequence.
ACCESSION   DADLTF010000127 DADLTF010000000
VERSION     DADLTF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.127"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..708)
                     /locus_tag="JN749_003207"
                     /pseudo
     CDS             complement(1..708)
                     /locus_tag="JN749_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014166.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
     gene            complement(726..1010)
                     /locus_tag="JN749_003208"
     CDS             complement(726..1010)
                     /locus_tag="JN749_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HAY7369527.1"
                     /translation="MKLKTLTFIVLASVAALTGCEDKTSVDWYMAHHDDMLAKYRECI
                     FNHNFVPTDCQNARSALHREKAKPDVAEGYKQILKDLDKARVQEPIADLN"
     gene            complement(1022..1732)
                     /locus_tag="JN749_003209"
     CDS             complement(1022..1732)
                     /locus_tag="JN749_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein VirB5"
                     /protein_id="HAY7369528.1"
                     /translation="MKTTLTALTVALCLFHVPAQAGIPVFDEVANASNIQQWAEKLQQ
                     WQQTVQHYQSQMNAYKQQLATATGIRDVQAFLNQAKNLTADIKNLKNRGISLNDLLTG
                     SGGSYAGELNRLYDKYKLFDNCPENASEAYRDSCKQMLLNQAVSVEDTSDIQEKISDT
                     LSDISNLASRIENAKDSKESQDLANVVSARSIQLNALTTQWEMSVKQSEMRNQLLAEQ
                     RRKAQAEQMRTAPIADLN"
     gene            complement(1757..4507)
                     /locus_tag="JN749_003210"
     CDS             complement(1757..4507)
                     /locus_tag="JN749_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7369529.1"
                     /translation="MTILNKALTRPAALWGVPLVPLLFTLCILTLAAVWSRFYWLLLL
                     LIPAFIELRRLAKEDIHFFDLKLLAFKTRGNRAATRHFGTKALVANQYDAIDVTEFIK
                     HMRLNESIPLSRYLPWSSHLHEQVVKNKKGDFVSTWEIGGIIFECEDEHYLATLDMQL
                     NNVIRSFEGLPVTFYTHLVSDEFTDTLECESGIPFSDEIARLFYASREGKPFYQRRLF
                     FTVCFNPHTGIERATMKQKSLGERKTLLDNALQVMQEHRQAIGTALSRYAARPLGTYE
                     ENGHVFSSQLAFYNFLITGRWQKVRVTRTPFYDVLGGTDIFPSTDIGQVSHTDGQSYF
                     RGLEIKDYSTETITGLYDSLLYARCRYVFTQSYTCMGKDEARGCIRTIEKRLKSAEDD
                     AISQQEDLKVARDMLQSGIISFGKYHQTLIVSAPTSEQVIRDTNTLADTLKDLGFIMT
                     LSRESLSAAYLAQLPGVYHLRPRLVPVSSLNYAEMVSFHNFHPHKRDGNAWGEAIAVV
                     PTPSGGCQYVNLHNSQADVDEFSEKPPGNTMLLGSTGTGKSTLLMMFQHLMQKYRRPE
                     SFAPGATKKRFTSVVFDKDRSAEMSIRLQGGSYFRFRSGEKTGLNPFALAPTKRNRNF
                     LKKLMRMMCTRDGKPLDPRDEERLSEAVDTIMLDYPPELRRNGITRMLEVLHEAPTKD
                     ARMNGLRIRLQQWAQGGEFGWVFDNDVDTFDIDNIDNFGIDGTEFLNDKDVRSPLTFY
                     LLYRVTSLLDGRRLVIFMDEFWKWLQDPEFSKFALDMLKVIRKLNGIFFPATQSPEEM
                     CKHEISAAIIEQCSTRIFMANQQAKEYDYVDMLRVPKAVYDIVTTLDPYARQMVIMKS
                     PLRRGDVRPYIAQITLDLSGLGRYTKLLSASADNLKIFDEIWQEGMKPEDWKDEMLRR
                     AV"
     gene            complement(4517..4816)
                     /locus_tag="JN749_003211"
     CDS             complement(4517..4816)
                     /locus_tag="JN749_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HAY7369530.1"
                     /translation="MTLRTIKTAATRLIAAFSALSAVPAMAASADQAFQKGSDAINWV
                     FLGLTGMSVVTISCAVAFIAYKMLVDGKAWHDLQRVFWAGVLLAGASGFGAYFAA"
     gene            complement(4827..5489)
                     /locus_tag="JN749_003212"
     CDS             complement(4827..5489)
                     /locus_tag="JN749_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HAY7369531.1"
                     /translation="MLTTAALFQLAMQCAPAVHPDTIHDITRTESGLNPYAIAEIVPV
                     KGGRSRVISHLPSSKDEALKIVEAIKQKKHRYSVGLMQITSTNFPQFGVSAESMFNPC
                     DNMTVAAKIITDCYQRGGTLQRALSCYYSGNFETGQRPESAFGNTSYVQRIGYVVPST
                     RAEQQTASPSSGEAPAVPSDNTVYPDSVIRGVIPAPDTTPTSIAAYPPHVVRGGLAVS
                     SD"
     gene            complement(5492..5707)
                     /locus_tag="JN749_003213"
     CDS             complement(5492..5707)
                     /locus_tag="JN749_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369532.1"
                     /translation="MPDITPAPASLLEAVFTPMTEYTHNVYSVKGDRLAMTLTKIGVA
                     QANSQGDIQLFINPDCATGKVLLYRKD"
     gene            complement(5700..6008)
                     /locus_tag="JN749_003214"
     CDS             complement(5700..6008)
                     /locus_tag="JN749_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369533.1"
                     /translation="MSMQTVRRKALRQNPYSTLGKPPLRSSRDRITRAINGTWSLREL
                     PDNVRELLIERAALRAEITTLTVKAQGLKKSDVSVLIADRLRRIDAITSLLENRKCNA
                     "
     gene            6322..7236
                     /locus_tag="JN749_003215"
     CDS             6322..7236
                     /locus_tag="JN749_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAY7369534.1"
                     /translation="MLEMFEKIEKKYWQDKIKKISKFRVIKMQDLDDAKVIEVLAKVL
                     TKKGISKVKHNSYISKVLQITTSAGYKKLNGATKWEVQQLIKVVQSVGMDMIEFFRIY
                     YEDNKEIQDAIWNDGRSEHSCKVCLYPEGSEVNTEYSALKVADQWNIFPTNDLKDELL
                     YESKRSIESIVINPKVISSTKYRIALLDDDANITESLKEILSNDYYQVDTYDNLKKLT
                     QAVATNPYDAYVLDWIIGDETSFKLVKAIRSSKNKNAMIIVLTGQLSGIKDDEIVKSI
                     HDYDIVGPYEKPIKSGIIKSNIEKYFAR"
     gene            7240..8223
                     /locus_tag="JN749_003216"
     CDS             7240..8223
                     /locus_tag="JN749_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HAY7369535.1"
                     /translation="MRNLLEYSGSLISNDSLIVAPYDCQKLSYENILPLNNGLIKNWN
                     IQKFSSDYSEIEHLNVINGMGVTLGDSIVGISVLSAIKKKNPKIKIKLIRPETAPSYV
                     EELYELASSVIDELHYMPFHIQKLPCTALNIDVGNQLYSPDFHIMEMHDYFIQGLGVK
                     LDDVNHNELSNKWLGNIDLGAPVFENYTLFAPIASTKIRSIPSKFYYDIVDMLSRREG
                     KTVLGFVDVNHKNYINISKYSPHTKDFIALIKYASNVYTCDSSALHIAAGFGVPTECV
                     FNTIPPELRTKYYSKCKSIYVGNKRLEKIQSSEDFQLVKMVENNYREYLYG"
     gene            8216..8704
                     /locus_tag="JN749_003217"
     CDS             8216..8704
                     /locus_tag="JN749_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7369536.1"
                     /translation="MVKIISFLSLMLLSISAYSNIGDIVIDGSIANANSDKGYVITEK
                     QFDSFPRDTIVTTTPWTPTGKKVKFKGVDLSYVLKLAGANGAKLKFHALNDYEIIVDM
                     NDVEKYNILLATEMDGKRLQIRDFGPYFLVYPLDEHYIELNTPHYLARFIWQIDKITV
                     LK"
     gene            8701..10419
                     /locus_tag="JN749_003218"
     CDS             8701..10419
                     /locus_tag="JN749_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HAY7369537.1"
                     /translation="MKKNFFIILVALLLLIAFTVSSVVKYTSLLSEKNLYTVAGTQEN
                     YGFSVAKFIIQLNELYTLINSDNTIDDVRLKFDILYSRLNVIYVKSEATAPLYKQQGY
                     EETIDSINKKLEDIDGLLSHNHPDYKKISTIIADIKPLTKTVTNLADMAEIAQRNDAL
                     KDFRSKRQQLWTLLFITGGLISLLLFVLFIYISKINRLLLSERAAFASKNAFLGMVGH
                     ELRTSLQAIVSIIDVVTNNLSGGIKSGQIERLETAVSKMERQLNDLAEFAKIDNGSVE
                     IKNTYNSLQAIVTNAVQDCIAIYEKKDVTVKIKNNNDAVIFTDALRLNQVIENLTSNA
                     IKYTERGEVNVDYFIEKGKVLNIVISDTGKGIPKDKLKFIFKPFTRVVDSKSTVPGFG
                     MGLAIVAGIIRLLKGTIHISSEVNVGTTVTVRIPIKLGDSSLATAEVSASAPGDVFGV
                     LSLLVIDDNEMACSSLSSLLTNAGYIVEATTSPERALEKLLRKPYDVVLSDLQMPGMT
                     GDELYATVSSSQNPNRSTPFIFISAYSDAHSIGQAPLLTKPVRLKDINLKIHEVMSNQ
                     GRVKSE"
     gene            10683..10892
                     /locus_tag="JN749_003219"
     CDS             10683..10892
                     /locus_tag="JN749_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369538.1"
                     /translation="MLLTLRGMFFSYFSDTRELNTVVAKLYPLKMSQMPLYAVGVVPA
                     RRQPQPGRISALAGRRAAYERVPDN"
     gene            11101..11235
                     /locus_tag="JN749_003220"
     CDS             11101..11235
                     /locus_tag="JN749_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369539.1"
                     /translation="MGALSDVAEPARPSGRCKSGHSPEPVHRVGDEEVMCLQDLLKDL
                     "
     gene            complement(11278..11498)
                     /locus_tag="JN749_003221"
                     /pseudo
     CDS             complement(11278..11498)
                     /locus_tag="JN749_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2997 a         2639 c         2934 g         2928 t
ORIGIN      
        1 ctgaacggcc agagtcaggt agttggcatc ggatgctttt gtggcgcccg ccaggatgta
       61 gtccaggtaa tccattacca ccgtcagggc gatgccgctg aacagggatg taaggatgca
      121 gcccagaata attctgagcc agttgctgaa catatcccgg aaccacgacc acatcaggca
      181 gtagataaag atcggggcgg tcgccagcat cagcagcatc accagttcgg ctatcaggtt
      241 aatgatggcc gtgatgccta ccagtaacat gacgcctgcc cagaccagaa actgcgccag
      301 agtgcctttt agagcgacat aggttgaatc atcataagag taaagcttct ccccaagtgc
      361 ttgagccttt tcccagacgg tatccagccg cgcctagatc gacgcgtcgc cgctgaatcc
      421 gtcccgcagg ccggtgatgg catccgaaac cagatcgaga tagcctccgg cgttggtgac
      481 gaaaatcatg atgatgagca tccggctcac gtcccacacg atctcctcga aggggcggac
      541 tagcttgcct gccatcgtct ggtaggcgcg ccacgtgatg tacagcgaga gcaccgcgat
      601 ggagaacagg tgtgccatcc acccccccgt atcggcggcc acgctggtga gcacgctgtc
      661 aaacccctcc atgatgtggc tgtttaatcc gacgaatatt ccactcatgg tgcgttctcc
      721 tgtcgctaat tcaggtcagc aataggctct tgcacgcgag ctttatcaag gtctttcagg
      781 atttgcttat atccttcggc aacatcaggt ttagcttttt cacggtgaag ggcggagcgt
      841 gcattctggc aatcggtggg cacaaaatta tgattaaaaa tgcactccct gtatttcgcc
      901 agcatgtcgt catggtgtgc catataccag tctacggagg ttttatcctc gcagccagta
      961 agcgcggcca ctgaggccag aacaataaag gtcagtgtct tcagtttcat ggggtcacct
     1021 gttagttaag gtctgcgatt ggagctgtgc gcatttgctc ggcctgcgcc tttcggcgtt
     1081 gttcggcaag cagctggttg cgcatctcac tttgtttgac cgacatttcc cattgagtgg
     1141 tcagggcgtt cagctggata ctccgcgcgg acactacgtt cgccagatcc tgcgattctt
     1201 tactgtcctt cgcattctcg atgcgactgg cgagattact aatatcgctc agcgtgtcgg
     1261 aaattttttc ctggatgtca gaagtgtcct cgacggacac cgcctgattg aggagcatct
     1321 gtttgcagct atcccggtac gcctcgctgg cgttctccgg gcagttgtcg aacagtttgt
     1381 atttgtcata cagcctgttt agttccccag cataagaccc gcctgagccg gttaacagat
     1441 cgtttaagga aatccccctg tttttcagat ttttaatgtc agccgtcagg ttttttgcct
     1501 gattcaggaa tgcctggacg tcccgaatac cggtggccgt tgccagctgt tgtttataag
     1561 cgttcatctg cgactgataa tgttgaaccg tctgctgcca ctgctggagc ttctccgccc
     1621 actgctggat gttgctggcg ttggctactt catcaaaaac gggaataccg gcctgagccg
     1681 ggacgtgaaa gagacacaag gcgacggtca gcgccgtcag ggtggttttc agtttcatgg
     1741 tgttatcctt ttgtgattaa acggcacgtc tcagcatttc gtctttccag tcttccggct
     1801 tcataccctc ctgccagatc tcgtcaaaaa ttttcaggtt gtccgcactg gcgctgagca
     1861 gcttcgtgta gcgacctagc ccggagaggt cgagggtgat ttgtgcgata tacgggcgca
     1921 cgtcaccacg ccgcagcgga gatttcatga tcaccatctg gcgggcgtag gggtcgaggg
     1981 tggtcacgat gtcgtaaacc gctttcggca cccgcagcat gtccacgtag tcgtactcct
     2041 tcgcctgctg gttcgccatg aagatgcggg tactacactg ctcgataatg gccgccgaga
     2101 tctcgtgctt gcacatctcc tccggcgact gcgtggccgg gaagaaaatg ccgttgagct
     2161 ttcgaatgac cttcagcatg tcgagggcga attttgaaaa ctccgggtcc tgaagccatt
     2221 tccagaattc atccatgaaa atgaccaggc ggcggccatc gaggaggctg gtcacgcggt
     2281 agagcaggta gaaggtgagc gggctgcgca cgtctttgtc gttcaggaac tccgtgccgt
     2341 cgatgccgaa attatcaata ttgtcgatat caaaggtgtc cacgtcatta tcaaacaccc
     2401 agccaaactc cccgccctgc gcccactgct ggagacggat gcgcagcccg ttcatccgtg
     2461 cgtccttcgt cggggcttca tgcagcactt ccagcatgcg ggtgatgccg ttgcgacgaa
     2521 gctcaggcgg gtagtccagc atgatggtgt ccactgcctc gctgaggcgt tcttcatcgc
     2581 gcgggtcgag cggtttgccg tcccttgtgc acatcattcg catcagcttc ttgaggaagt
     2641 tgcggttacg tttcgttgga gcgagcgcaa acgggttaag gccggtcttc tcgcctgaac
     2701 ggaaacggaa gtacgagccg ccctggaggc gtatcgacat ttccgccgaa cggtctttat
     2761 caaagaccac cgaggtgaag cgctttttcg tcgcccccgg cgcaaacgac tcaggacgcc
     2821 ggtatttctg catcagatgc tggaacatca tcagcagggt cgatttcccc gtcccggtac
     2881 tgccgagcag catggtgtta cccggcggct tctcgctgaa ctcgtcaaca tcggcctggc
     2941 tgttgtgcag attcacgtac tgacatcccc cggacggggt cggaacaacg gcaatcgcct
     3001 ccccccaggc attcccgtcc cgcttgtgcg ggtgaaagtt gtgaaacgac accatttcgg
     3061 cgtagttgag gctgctcacc ggcaccagac gcgggcgcag gtgatacaca cccggcaact
     3121 gcgcgagata cgccgccgac agcgactcgc gggacagggt catgatgaaa ccgagatcct
     3181 tcagggtatc cgccagggtg ttggtgtccc ggataacctg ctcgctggtt ggggcgctga
     3241 caataagcgt ctggtgatat ttcccgaatg aaataatgcc ggactgaagc atgtcgcggg
     3301 cgaccttcag gtcttcctgc tgggagatcg catcatcctc ggcagacttc aggcgtttct
     3361 cgatggtgcg gatgcagccc cgcgcttcat ccttacccat gcaggtatag gactgcgtga
     3421 acacgtaccg gcagcgggca taaagaagcg agtcatacag cccggtgatg gtttcagtgc
     3481 tgtaatcctt gatttccagc ccgcgaaagt agctctggcc gtcggtgtgg ctgacctggc
     3541 caatgtcggt ggaagggaaa atatccgtgc cgcccagcac gtcataaaac ggcgtgcggg
     3601 tgacgcggac tttctgccac ctgccggtga tcagaaagtt gtaaaacgcc agctgggagg
     3661 agaacacgtg cccgttctcc tcataagtgc caagcggccg cgccgcgtag cgtgacagcg
     3721 ccgtgccaat cgcttgccgg tgctcctgca tcacctgcag ggcattgtcg agcagggttt
     3781 tacgttcgcc cagggacttc tgtttcatgg tcgcccgctc aatgccggtg tgcggattga
     3841 agcagacggt gaagaagagg cgacgctggt agaaaggttt gccctcgcgg gaggcgtaaa
     3901 acagacgcgc gatttcatct gaaaacggaa tcccggattc acattcaagc gtgtcggtaa
     3961 attcgtcgct caccagatgg gtatagaagg taacgggtag cccctcaaag gagcggataa
     4021 cgttgttgag ctgcatgtcc agggtggcga ggtaatgttc atcctcgcat tcaaaaatga
     4081 taccgccgat ctcccaggtg ctgacaaagt cacctttttt gttcttcacc acctgctcgt
     4141 gcaggtggga agaccagggg agatagcggg acagcgggat actctcgttg agccgcatat
     4201 gttttataaa ctccgtaacg tctatggcgt cgtactggtt tgcgacaagc gcctttgtcc
     4261 cgaaatgccg ggtcgccgcg cggttgccac gcgttttaaa cgccagcaac ttcaggtcaa
     4321 agaaatgaat gtcctctttt gccagccgtc tcagctcgat aaaggcaggt atgagaagca
     4381 ggagaagcca gtaaaaacgt gaccagacgg ccgccagggt aaggatgcac agggtgaaca
     4441 gcagggggac aagcgggacc ccccacagcg ccgccggtcg ggtcagcgcc ttgttgagga
     4501 tggtcatgct gcctccttag gcggcgaagt aggcaccgaa cccggacgcc ccggcgagca
     4561 ggacacccgc ccagaacacg cgctggagat cgtgccaggc cttgccgtca acaagcatct
     4621 tgtaggcgat aaaggccacc gcacagctga tagtgacgac tgacatacca gttaggccga
     4681 ggaagaccca gttaatggcg tcactgcctt tctgaaatgc ctggtcagcg ctggccgcca
     4741 ttgctggaac agcagacagg gcggaaaatg cggcaatcag gcgggttgca gcggttttga
     4801 tagtacgtaa agtcatgatc tcttccttaa tcagaagaca cagcgaggcc gccgcgcacg
     4861 acatgtggcg gataagcagc gatgctggtc ggggtggtgt cgggtgccgg aattacgccg
     4921 cgtatgacgc tatccgggta aacagtgttg tcggacggga cggcaggcgc ttccccggat
     4981 gagggagatg ctgtctgctg ttctgcccgc gttgagggca caacgtagcc aatacgctgt
     5041 acgtagcttg tgttgccgaa cgcgctttcc gggcgctggc cggtttcaaa gttgcctgag
     5101 taatagcagc tcagggcgcg ctgtaatgtt cctcctcgtt gatagcagtc ggtgatgatt
     5161 ttcgcggcaa cagtcatgtt gtcacagggg ttaaacatgg attcggcgct tacgccgaac
     5221 tgcgggaagt tggtgctggt tatctgcatc agccctactg aataacggtg ctttttctgc
     5281 tttatggcct ccacaatttt tagcgcctcg tctttcgacg aaggcagatg agaaatcacg
     5341 cggctcctgc cgccctttac cgggacaatc tcagcgatgg catatggatt taagccggat
     5401 tcggtgcggg tgatgtcgtg gatggtatcg gggtgaacgg cgggagcaca ctgcattgcg
     5461 agctgaaaca acgcggcagt ggttaacata gtcagtcctt tctgtagagc agtaccttac
     5521 cggtggcaca gtccgggttg ataaataact gaatgtcgcc ctggctgtta gcttgggcaa
     5581 caccgatttt tgtaagcgtc attgccagcc gatcaccctt caccgaatac acgttgtgcg
     5641 tgtactcggt catgggggtg aatacggctt caagcagtga ggccggagca ggcgtgatgt
     5701 caggcattac atttcctatt ttccagcagt gaggtgattg catcgatacg tcgcagcctg
     5761 tcggcaataa gtacggatac atcggatttt ttgagtccct gcgctttcac agtcagggta
     5821 gtgatttctg cccgcagggc tgcccgttca attagcaatt ctcggacgtt atccggcagc
     5881 tccctgagcg accaggtgcc attaatggca cgcgtgatac ggtcacggga ggagcgtagc
     5941 ggcggcttgc cgagagttga ataaggattc tgacgcagcg ccttacggcg gacggtttgc
     6001 atgctcatgg ctgtttctcc tgggtatgag taagccaccc gcgtgcggtg cacagtggga
     6061 gacagggaag gattaaacgg gaggcttaac gatacagagg agtgagagtg ttacccatta
     6121 caggcgcggc acgtcatctc tttacgcagt cacagaaaac acctccttat cctgaccggg
     6181 gaacgccgct gctaggttat taaagagcta atgatttgat taatacaata gtgcttataa
     6241 gttgggtatt caataaaatt ataacttata gtatcgattt aagaatgatt ccaattttta
     6301 ggactttttc agctgtgtaa aatgttggag atgtttgaaa aaattgaaaa aaaatactgg
     6361 caggataaaa tcaagaaaat ctccaaattc agagtaataa aaatgcaaga tctcgatgat
     6421 gcaaaagtga tagaggtact ggcgaaggta ttgaccaaaa aaggtatttc caaggtaaag
     6481 cacaatagtt acataagtaa ggtgctacag atcactacat cagcgggcta taagaagttg
     6541 aatggtgcga ccaagtggga ggttcagcaa ctcataaaag tagtccaatc cgttggcatg
     6601 gatatgattg agtttttccg tatttattat gaggataata aagaaattca agatgcgatc
     6661 tggaacgatg gccgcagtga acactcttgt aaggtatgcc tctatcctga aggttcggaa
     6721 gtaaacacag aatactcagc actgaaagta gctgaccaat ggaacatttt tccgacaaat
     6781 gatttaaaag atgaactgct ttatgaatca aaacgaagca tagaaagtat tgtcataaat
     6841 ccaaaggtaa ttagcagcac taagtatcga attgcactac ttgatgacga tgctaatatc
     6901 accgaaagct taaaagaaat cctttcgaac gattattatc aagtagatac ttatgataac
     6961 ttgaaaaaac ttactcaagc tgtcgcaaca aatccgtatg acgcatatgt tttggactgg
     7021 atcatcggtg atgaaacaag ctttaagttg gttaaagcta taaggtcgtc aaaaaataaa
     7081 aatgcaatga taatcgttct tacaggtcaa ctatctggta taaaagatga tgaaattgta
     7141 aagtcaattc atgattatga cattgtggga ccatacgaaa agccgattaa atcaggcatc
     7201 atcaaatcta acattgaaaa atatttcgcc agatagatta tgagaaattt acttgaatat
     7261 tccggtagct tgataagtaa cgatagtttg atcgtagcac cttacgattg tcagaagcta
     7321 agctatgaaa atattttacc attaaacaat ggtcttataa aaaactggaa tatacaaaaa
     7381 ttctcaagtg attactctga aatagaacac ctaaatgtga tcaatggcat gggagtgaca
     7441 ttaggtgatt ctatcgtcgg gatttctgtg ctgtcagcaa tcaaaaaaaa gaatcccaag
     7501 attaaaatta agcttattcg accggagact gcaccgtcat atgttgagga actgtatgaa
     7561 ttagcatcct cggttataga tgagcttcat tacatgcctt ttcatattca gaaattgcct
     7621 tgtactgcat tgaatattga tgtgggaaat cagctatatt ctccagattt ccatattatg
     7681 gaaatgcatg attattttat ccaaggtctt ggcgtgaaac ttgatgatgt taaccataat
     7741 gagctatcta ataaatggtt aggcaatatt gatctcggag cgccagtttt tgaaaactac
     7801 acactttttg cacctattgc tagcacaaaa ataagaagta taccgtcgaa gttctattat
     7861 gacatagttg atatgctttc taggcgcgaa ggaaaaacgg tactaggttt cgtggatgtg
     7921 aatcataaaa attatattaa catatcaaag tattcacccc atacgaaaga tttcatagcg
     7981 ctaattaagt atgctagcaa cgtttatact tgtgactcct ctgcattgca tatagccgct
     8041 ggatttggtg tacctactga atgcgtgttt aatacaatac cgccagaatt aagaacaaag
     8101 tattacagta agtgtaagtc gatttatgtt gggaataaaa gattagaaaa aattcagagt
     8161 agtgaagatt tccaactggt taaaatggtg gaaaataatt acagggagta tctttatggt
     8221 taaaataata tcattcttat ccctaatgct tttgtcaatt agtgcatata gcaatattgg
     8281 ggatatagtt atagatggtt caatcgctaa tgctaacagt gataaaggtt atgtgattac
     8341 tgaaaagcag tttgattcgt tcccacggga taccatcgta acgacaacac cttggacgcc
     8401 aaccggtaaa aaagtaaaat tcaagggcgt cgacctaagt tatgttttaa aactcgctgg
     8461 cgctaatgga gcaaagctaa aattccatgc tctgaatgat tatgaaatca ttgtagacat
     8521 gaatgacgtt gaaaaatata acattttatt ggctacagaa atggatggaa aaaggcttca
     8581 aataagggat tttggacctt attttttggt atatccttta gatgaacatt atattgagct
     8641 taacacgcca cactatcttg cacggttcat ttggcagata gacaaaatta cggtgctcaa
     8701 atgaaaaaaa atttctttat cattttggtt gctctgcttt tgttgatagc attcactgtg
     8761 tctagtgttg ttaagtacac cagtctttta tcggagaaaa atttatacac cgtcgctggc
     8821 acccaagaaa actacggttt ctcagtggca aaattcatta tacagttgaa tgagctttac
     8881 acgctaatca atagcgataa tactattgac gatgttaggc ttaaattcga tatcctttat
     8941 tctcgtttga atgttattta tgtaaagagt gaggcgactg cacctctata taaacagcaa
     9001 ggctatgaag aaacaattga ttcgataaac aaaaagcttg aagacataga cgggctgctt
     9061 tcgcataatc accctgatta taaaaaaata agtacaatca tagctgatat aaaaccgcta
     9121 actaaaaccg ttacaaatct tgctgatatg gctgaaatag cacaaaggaa tgacgctctt
     9181 aaagatttca ggagcaaacg tcagcagtta tggactctgc tttttattac aggtgggttg
     9241 atttccttac ttctttttgt cctatttatt tacataagta aaattaaccg attgttacta
     9301 tcagaacggg ctgcttttgc cagtaaaaat gcatttttag gtatggtagg ccatgaactc
     9361 agaacatctt tacaggcaat agtgtctatc attgatgtcg taaccaataa tctgagtgga
     9421 gggattaaat ctggtcagat agaacgactt gaaactgctg tttcgaaaat ggagcgccag
     9481 ttaaatgatt tggctgaatt tgcaaaaatt gacaacggat ctgtagagat aaaaaacacc
     9541 tataatagct tgcaagcaat tgttactaac gctgtgcagg attgcattgc tatttatgaa
     9601 aaaaaggatg tcacagttaa aataaaaaac aataatgatg cagtgatttt tacagatgct
     9661 ctacgcctta atcaagtaat tgaaaactta accagtaacg ctattaagta cactgagcgc
     9721 ggggaagtta atgtagatta ctttatagaa aaaggtaagg tattaaatat tgtcatttct
     9781 gataccggaa agggaatccc taaagacaaa ctaaagttca tatttaaacc atttacacga
     9841 gttgtagatt cgaaatccac tgtacctgga tttgggatgg gacttgctat tgttgcaggg
     9901 ataatcagac tattaaaagg tactattcat atatctagtg aagtaaatgt aggtacaact
     9961 gttacagtga gaatacctat caagttggga gatagcagcc ttgcaacagc tgaggtatct
    10021 gcttcagctc ccggagatgt cttcggcgtt cttagcttgc ttgttattga tgataatgaa
    10081 atggcctgtt cgtctctttc cagtctgctt actaatgctg gatacattgt cgaggctaca
    10141 acctcgcctg aacgagccct ggaaaagctt ctgcgtaaac cttatgacgt tgtcctgagt
    10201 gatttacaaa tgccaggcat gacaggtgat gagctgtatg caactgttag tagcagtcag
    10261 aatcctaatc gatccacgcc tttcatattc atcagcgcat acagtgatgc acactctatc
    10321 ggacaggctc ctttgttaac taaacctgtc aggttaaaag atataaacct taaaatacat
    10381 gaggttatgt caaatcaagg acgagtcaaa agtgagtagg cattgtcgta atatttgcga
    10441 ttatagcttt ggtgttctcg atcgttgcga attttgaaaa tttgtggagt caaatggcgc
    10501 cgaaagcccg gcgtagccgg ttacctggct ataaactctg ccaccctacc ccctgccctg
    10561 attagcctta acccgcttac agcaatgaag aagtaaaagt aatccctttt aaccccggtg
    10621 agcgggcttt acggcgttta cgacgtaaag ggcgtgattt ccggggcctt agcggcggca
    10681 gcttgctgct gacgcttagg gggatgttct tcagttattt cagcgacacc agggaactta
    10741 acacggtagt tgcaaagcta tacccgctaa aaatgtctca gatgcctctg tacgcggtcg
    10801 gagtggtccc ggcaaggcgt cagccgcagc cggggcgtat ctccgcgtta gcgggccgaa
    10861 gggccgctta cgagcgtgta cccgacaact gaccgcagct ccacccacag cgatgaagaa
    10921 gagtagttac aacacccatc attaaaagcg acactactcc cggccttcgg ccgggggcgg
    10981 cggcgctttt cagtggtggg gaggggcacc cggccggcaa accggcgccg gttttatgat
    11041 gagctttctt ttgaatgctg taactaattt ggttctccgg tcggggccct tcgcgaagcg
    11101 atgggagctc tttctgatgt tgccgaaccg gcgcgaccgt caggtcgctg caagagcggg
    11161 cattctccgg agcctgtcca cagggtaggc gatgaagagg ttatgtgttt acaggattta
    11221 ttaaaggatc tttaaaagag atacagaact ggtgtttaaa aggaactacg cgccctgaaa
    11281 gaccgacggc aggctcagaa gatgctccag cgtggccata gtacgttcac cgaagacgtg
    11341 tgcaacaacc gtcctccgta tcctgtcata cgcgtaaaac aaccagcgct ggcgtgattt
    11401 agcgccgacg taaccccact gttcgtccat ttccgcgcaa acaatgacgt cactgcccgg
    11461 ttgtatgcgt gagtttaccg actgcggcct gagaggcg
//
LOCUS       DADLTF010000128        11438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.128, whole genome shotgun sequence.
ACCESSION   DADLTF010000128 DADLTF010000000
VERSION     DADLTF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.128"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            9..461
                     /locus_tag="JN749_003222"
     CDS             9..461
                     /locus_tag="JN749_003222"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7369540.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            707..1751
                     /locus_tag="JN749_003223"
                     /pseudo
     CDS             707..1751
                     /locus_tag="JN749_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1867..2427
                     /locus_tag="JN749_003224"
                     /pseudo
     CDS             1867..2427
                     /locus_tag="JN749_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JN749_003225"
     CDS             complement(2484..3941)
                     /gene="pepD"
                     /locus_tag="JN749_003225"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7369541.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4203..4661
                     /gene="gpt"
                     /locus_tag="JN749_003226"
     CDS             4203..4661
                     /gene="gpt"
                     /locus_tag="JN749_003226"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7369542.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4753..5997
                     /gene="frsA"
                     /locus_tag="JN749_003227"
     CDS             4753..5997
                     /gene="frsA"
                     /locus_tag="JN749_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7369543.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6055..6456
                     /locus_tag="JN749_003228"
     CDS             6055..6456
                     /locus_tag="JN749_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7369544.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JN749_003229"
     CDS             complement(6495..7550)
                     /gene="phoE"
                     /locus_tag="JN749_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7369545.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7838..8941
                     /gene="proB"
                     /locus_tag="JN749_003230"
     CDS             7838..8941
                     /gene="proB"
                     /locus_tag="JN749_003230"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7369546.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8953..10206
                     /gene="proA"
                     /locus_tag="JN749_003231"
     CDS             8953..10206
                     /gene="proA"
                     /locus_tag="JN749_003231"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7369547.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10321..10396
                     /locus_tag="JN749_003232"
     tRNA            10321..10396
                     /locus_tag="JN749_003232"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10354..10356,aa:Thr,seq:cgt)
     gene            complement(10411..>11438)
                     /locus_tag="JN749_003233"
     CDS             complement(10411..>11438)
                     /locus_tag="JN749_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7369548.1"
                     /translation="WRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLH
                     FREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKT
                     RHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVV
                     MFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQG
                     LHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWAS
                     WLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEEN
                     VVYLNAR"
BASE COUNT         2744 a         2838 c         2946 g         2910 t
ORIGIN      
        1 tttatattat gaataacata caaataagag attatcagcc tggcgatttt cagcaactat
       61 gcgctatttt cattagagcg gttacgatga ccgtcagtca gcattattca ccacaacaaa
      121 ttgccgcctg ggcgcagatt gacgaatctc gctggaagga gaaactcgcg aaatcacaag
      181 tgcgggttgc agtcattaat gcaaaactgg tcggctttat tacctgcgtt gaacactata
      241 tcgatatgtt atttgttgac cctgaataca cccgccgtgg ggttgccagc gctttgttaa
      301 aaccttttat taagtctgaa tccgaactta cggtggacgc aagcataacc gcaaaaccct
      361 tttttgaacg ttatgttttt cagacagtta agcagcagcg cgttgaatgc cggggagcgt
      421 ggtttattaa tttttatatg cgttataaac cgcaacatta aatccagctt gcaatgaaaa
      481 taacgcccgc ctagtatgtg cgcgttttct tatttcacaa actgcaagga ggtaaatcat
      541 gacaaatcct ttatcaatga ctctttgcag acctttccag gattaattct ttttttcttg
      601 ccctggattc gtctgccatt tcctgatttt ttaattgtta ctctcaataa ttcaagtcgc
      661 agcacatgca acttgaagta tgacgagtct atttacggaa tggtatatgg gcaattatat
      721 tcgtccctta tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt
      781 agcgatccaa caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat
      841 gccagattta caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg
      901 tttttaccca gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga
      961 tattctcgct cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga
     1021 cgttgctgaa gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg
     1081 gcgcagctcg cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga
     1141 tcaaatcatc aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact
     1201 gctggttcat agcggctcga gggagctagg gcagtctatt ttacagcggc atattgcctc
     1261 gttttcgcat catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa
     1321 tcgccacctg atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct
     1381 ggatgtggcg cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca
     1441 tcgtaaaggt gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga
     1501 ttactccagg ctggttcagg cccgtcagga gtgaggaaac attgcattcg ctggcgcatg
     1561 gggctgggcg taaatggggg cgcaccgagt gtaaagggcg tctggcagcg aaatacacag
     1621 cgacgcaact ctcgcgtact gaacttggca gtcgggtaat ttgtcgcgat aaacaactca
     1681 tcttcgaaga agcgccacaa gcctataaat cggctgaaag tgtggtgcaa tgtctgggca
     1741 ggccggatta attattcctg tcgcgcggct gcgtccggtg ctaacgctca aaaacagtgg
     1801 agggaaaaaa ggatgatctt gctataactc tcctcagcgc aggggccgga agaatgttgt
     1861 ctcgcagtga aaaaggcgct ggacaggcta attaaagaag ctgcccgaca agacgtcgcg
     1921 gtaacggtgc tggaaacaga aacaggccgc tactctgaca cgctgcgttc ggcgctgatt
     1981 tctctggatg gcgacaacgc atgggcgtta agcgaaagct ggtgtggcac tattcagtgg
     2041 atttgcccga gcccgtatcg cccacatcac gggcgtaaaa actggtttct gggcattggg
     2101 cgttttaccg ctgatgagca ggagcaatcc gatgcgatcc ggtatgagac gctgcgttcg
     2161 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatttg
     2221 gcatccggta ttagcgtgaa ggttcagtca gagcgcagcc agcatgctaa caaacggctg
     2281 gcgcgattgc tgattgcctg gaagctggat cagcagcaat aggaaaatag cgcggtgctg
     2341 aaatcacagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     2401 acagggatgg cttttatcga aggataatga aggatgaaac tgccggaagg cgattaaacg
     2461 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     2521 agtgtccagt aatgacctac gctttcgatg tgaacttgct catccggaga gtgtggaccg
     2581 gtgatagttg gcccgataga aaccatgtcc atttccggat acggtttttt gaacagacca
     2641 cattccaggc ccgcgtggat aatctggatg ttcggcgtct tgttgaacag gcgctgatag
     2701 gtttcacgta ccagatgcat caccggagaa ttagcgtccg gctgccagcc aggatatgcg
     2761 cctttcgctt cggttttcgc gccagccagt ttacccagcg aatccagcat gctcaccacg
     2821 tagtctttac cgctgtcgat cagtgaacgg atcaggcagt gaatttctac gttattgtca
     2881 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggaa
     2941 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     3001 gcaatcaggg cagctttatc gttcgctaca gagtccagca acaaggccag atttttctct
     3061 ttctctgcca gctcgttttt caggatctcc tgataggtat tcaccagaga tttcagtgcg
     3121 tcgactttat cagctgtgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     3181 agtgtgccgc cgttgaaatc gataaggcgc aggtccagtt cttctgcatg acccgccagg
     3241 aagcgcacca gcagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     3301 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     3361 cgatctaaat gcaggttgga ggtgaagtcg ataccccccg cacaacccat gtagatttca
     3421 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     3481 ccgaacgcac cgtccatacc ggcttcttcg gttatggtca gcagcacttc cagcgggccg
     3541 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     3601 gcacccagcg tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga
     3661 tctttcgtga agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc
     3721 tgtaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     3781 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     3841 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     3901 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     3961 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     4021 ggcttgcgag atatgtattg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
     4081 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatatc tataatctcg
     4141 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
     4201 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
     4261 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
     4321 tggtaccggg ggcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta
     4381 tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg
     4441 atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact gctgttgcga
     4501 ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc
     4561 cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggatcgaa cagccgtggg
     4621 atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcat
     4681 tgccgggcgt tgttcttttt aacttcaggc aggttacaat agtttccagt aagtattctg
     4741 gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa
     4801 catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag
     4861 tcggctcttg atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg
     4921 atctggcgcg gtattgaccc acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc
     4981 gatgctgaac gtaccgacga tgatttatac gataccgtga ttggctaccg tggtggcaac
     5041 tggatttatg agtgggccac ccaggcgatg gtgtggcaac aaaaagcctg tacggaagaa
     5101 gatccgcaac tcagtggtcg acactggctg catgcggcta cgttgtacaa cattgccgcc
     5161 tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc
     5221 tatgaagagg ccgcccagcg tctgccgggc acgatgcggc agatggagtt taccgtaccc
     5281 ggcggtgcgc ccatcaccgg ctttttgcat atgccgcaag gcgatggccc gttcccgaca
     5341 gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct gtatgaacgt
     5401 tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt gggcttttct
     5461 tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtctt aaaggcgctg
     5521 cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg tttcggcgct
     5581 aacgttgccg tgcgtctggc ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt
     5641 ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt gccggaaatg
     5701 tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga agcgttacgc
     5761 gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg ctgcccaacg
     5821 ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga ctcacgctta
     5881 atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc ggtgtatcgg
     5941 aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa
     6001 tttgctaaat tttgccaatt tggtaaaaca gttgcatcac aacaggagat agcaatgacg
     6061 ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact aggcccgtat
     6121 attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc tgtatgcgtc
     6181 aacgtgaaac cggcaccgga agtgcgtgaa ttctggggct ggtggatgga gctggaagcg
     6241 caggaatccc gttttaccta cagctaccag tttggtctgt tcgataaagc aggcgactgg
     6301 aagagtgttc cggtaaaaga tactgaagtg gttgaacgac tggagcacac cctgcgtgag
     6361 tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga accggcggat
     6421 gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca
     6481 tccggcaata ttcattaaaa ctgatacgtc atcccaaccg cgacagtatc atcattatta
     6541 atattcagct tattatcgct atccagtttg ttgattttat aatcaacaaa cgctgacata
     6601 tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc ttcatcaccg
     6661 atgccctcaa tatctttccc tttcgataag acataaccca gcgatggacg cagaccaaag
     6721 tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt
     6781 attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt attggcatcg
     6841 tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct ttgcaggttc
     6901 tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc gctgccgcca
     6961 aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac gtcgcggttt
     7021 tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc gaagaagtcg
     7081 gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc ggtctgcgcc
     7141 gaggaatcgc caccaaattc cgggaacata tcggtccagg cttccacgtc atacaacgcg
     7201 cccaggttac gaccataatc gaaagaaccc aaatctttat atttcaaccc ggcaaaagcg
     7261 agacgcgttt tttgctgtgc agtatcactc tccgctttat ttccggcaaa ctccgcttcc
     7321 caccgaccat aaccagtcag ttgatcgtta atttgagttt cacctttaaa accaaaacgg
     7381 atataactct ggtcgccatc tttactggcg ttatcactca tataatgcat ggctttaact
     7441 ttgccataga catccagttt attaccgtct ttattatata tttctgcagc ctgtacagat
     7501 gcagatgcca caatgcccat caccactaat gccagagtgc tctttttcat tttcattcct
     7561 gattttaatt aacgcgcgaa tattcagcgg gagagccccg ttgaaaacag gaaagttttt
     7621 aacctgagat tgttaaagat atattacaga ttaatgatat tctaaaaatg tggtaattta
     7681 ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc gcgcaacaaa
     7741 acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa aacgttcgtt
     7801 tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc agacgctggt
     7861 ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc gtgcccatat
     7921 cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga ttgttattgt
     7981 gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac tgccagcgac
     8041 tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc aactgtggga
     8101 acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc gtgctgatat
     8161 ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc tcgatcacaa
     8221 tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta aggtcggcga
     8281 taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac tgttgctgct
     8341 gaccgatcaa aaaggtttgt ataccgctga tccgcgcagc aatccgcagg cagaactgat
     8401 taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca gcgtttcagg
     8461 cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt gccgtgcggg
     8521 tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg tgatggaagg
     8581 catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc gtaaacgctg
     8641 gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa ctgccgccat
     8701 tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg gcaatttctc
     8761 gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc acggtgtcag
     8821 tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag aaattgatgc
     8881 aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga ttacccgtta
     8941 aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc gtataaatta
     9001 gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga tgaactggaa
     9061 gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc gcgagccaat
     9121 ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct gaaaggcatt
     9181 gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt aatcgatggc
     9241 ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg ggttattggc
     9301 gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg cctgaaaacc
     9361 ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc ggcaacggtg
     9421 gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt gcaggcgatt
     9481 gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata catcgacatg
     9541 ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc gacaatcccg
     9601 gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagcgc agagatcgct
     9661 gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg taatacggtt
     9721 gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt aagcaaacaa
     9781 atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca gttgcaggca
     9841 ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt tctgtcatta
     9901 gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat tcgtgaacac
     9961 ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca gcgttttgtt
    10021 aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac cgacggcggc
    10081 cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc gcgtggccca
    10141 atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta caccattcgt
    10201 gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc attgacgcat
    10261 cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg tcctttcagg
    10321 gccgatatag ctcagttggt agagcagcgc attcgtaatg cgaaggtcgt aggttcgact
    10381 cctattatcg gcaccattta aatcaataag ttacctcgca tttaagtaaa ccacgttctc
    10441 ctcttgtgcc gtatttgtgc cattgcgact tataatcgca tcgattttgc ttgcgtgctc
    10501 ggtgagatgc ccggctgaaa ggtgggcgta tctttgaacc atttcgagag tttcccatcc
    10561 tcccatctct ttaagtgcaa gaagagagac accggactga accagccagc ttgcccaggt
    10621 atgcctcaga tcatggaagc ggaagttgct aatgcctgcc cgctttaacg ctcccttcca
    10681 tgccttgttg ctgtcggttc tcatcttcct taccgctgct gtttttgttc cgtcgcttcg
    10741 gtaggcaggt ttggtgtgga caaataccca tctcttatgg agcccctgct gttttcttaa
    10801 tatctggcat gcggtttcgt taagaggaac tccgatcgca ttgccagctt ttgtttcatc
    10861 agggtgcatc catgccattt tcttatccag atcgacctgt gaccactcaa ggtctgtaac
    10921 gttggaacgg cgaaggcctg tcgtgattgc aaacatgacc acagggaaga agtgaggagc
    10981 aatttctgca aacaggcgct tcgattcctc ctctgtaagc catctgatgc gtccattctt
    11041 aacgcgtggt gttgatattt tgggtgccct gtcaagccat ccccattcaa cagccatatt
    11101 gagaatggcg cgaagtattg ccagatgcct cgtcttcgtt cctttgcttg caagctttgg
    11161 tttatactcc ggcaccggat tgccaagtcg caaacacctg tcccggctca tctcccagtt
    11221 caggcgatgg cggcggtttt ccatcccgtc taccgcctcc attatttttt ctgttgttat
    11281 gtcagagaga atggcttctc tgaagtgcaa catccagaac gatataatgc tcttgtcatc
    11341 atcaatggac ttcttatccg atttctcacg cagccaccgt atgcaggctt ccttgaatag
    11401 ctttttcggt gattccccga gattttttac tctccacg
//
LOCUS       DADLTF010000129        11241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.129, whole genome shotgun sequence.
ACCESSION   DADLTF010000129 DADLTF010000000
VERSION     DADLTF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.129"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            4..435
                     /locus_tag="JN749_003234"
     CDS             4..435
                     /locus_tag="JN749_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369549.1"
                     /translation="MTMNLHTPTTAPKAAPATSVSPLEQSGSPKTKFPEIKTVKTAVV
                     KLISDIEGNLDQRLVYREAVEGLCQEAANRITQCMHSRSTGQTLRDLRELLSVTLDTI
                     IVVEFEGEQTRLNYGGRSAEDVIAMLKALHLEGRKAWLEVE"
     gene            complement(467..994)
                     /locus_tag="JN749_003235"
     CDS             complement(467..994)
                     /locus_tag="JN749_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HAY7369550.1"
                     /translation="MRKTTLLVLAAALCVPAFATAAPVLTAGFSPSDGRPALEIVLGA
                     INNARQSIDVAAYSFTSKPVATALAGANRRGVAVRVVADEKANSDRYTAVTYLINQGV
                     PVHLNGRYAIMHNKFMVIDGKNVQTGSFNYTASAVSRNAENVLLIEDAPQLAEAYQRE
                     FNRLWDEGIPLNALY"
     gene            complement(987..1733)
                     /locus_tag="JN749_003236"
     CDS             complement(987..1733)
                     /locus_tag="JN749_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HAY7369551.1"
                     /translation="MLISSYQERYARSTEKIRVLLNFLKEETYSDFQIIKLLFDFKSD
                     RPLYRLLEKVEKMGLIQKHVHESRASRISLWGITTDGLAVILTADDNIMPARFEPYRL
                     TGWSLDHHLDNQLVRLTLEKNGATGWINGDRSTFLSQYSVKHRPDGLISLPDGRLIAV
                     ETERRIKTKARYQSIMASHLLAISDKKWRYAFYVAPDEAKKRALKIIFDSIASVIVKN
                     QHVQLEAKHRDVFRFYTLDELKNLELDNYA"
     gene            complement(1748..3655)
                     /locus_tag="JN749_003237"
     CDS             complement(1748..3655)
                     /locus_tag="JN749_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAY7369552.1"
                     /translation="MIFDKLNHLLSPIANAVLAFIAFLQAHQLALALLSGVMLPFIFS
                     AKHGGQEKPTFLQRILILLSMICFVFGSIAPVAVWFLQRIYGREDIASPPLLTWTMAL
                     LFTVAGFILHVVLRRLISPEFDKVKCGMVKKTSMERDRRTDVRRVKEILPVTTEYDPM
                     DYIDLNKGIFIGLSREGEPQYIPLKEWQTQHADIIGTTGAGKGVVTGILLYQSILADE
                     GVFVLDPKNDEWAPHLYKKACADAGKPFVLIDLNKQEYQMNLIDGITADHLEELFVAG
                     FSLAEKGEAADFYRIDDRKAARTAAQFVKDNPGATVRDIYNGDYVQGIGETIKAFHGK
                     TEELAMLNSINAPGGFALKEIFDKGGCCYIIGSMRNSKIITAQRMILVRLFQLAELRD
                     RVAGNIRPIAIFKDEVKYHLSKPALEGLGAARDKGVHILMAHQSIADLRDCPADLNGD
                     AVVGAVVENCKFKLVYKLQDPDTAEWIARMSGTILVDDESRKVTTTAVLTEKVDNERM
                     LRQAESYYIDSNMLQNLPKFVSYIFSQNALAFPSLISPLKVKKAPLELFTLPPVAVAT
                     VAEVKPVLDLDFDKTAPAVPAPDIIPVSATTTKKPSATPIPTPPVQPDEEWLMASQPA
                     PNDEIHSLLDL"
     gene            complement(4364..4756)
                     /locus_tag="JN749_003238"
     CDS             complement(4364..4756)
                     /locus_tag="JN749_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HAY7369553.1"
                     /translation="MNRIQDELTSERALKKFLRESDFAFLEMLSARISAALDEKRAEH
                     IEQEKLRRQREEKRNELLALIESEGFSPTELVSTTPAKKSAKRKAKYQYFENGVHKKW
                     SGVGRVPVVIQQELDAGKPLESFLITTD"
     gene            complement(4753..6954)
                     /locus_tag="JN749_003239"
     CDS             complement(4753..6954)
                     /locus_tag="JN749_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HAY7369554.1"
                     /translation="MRLWIAEKPAVASDIAKALGGNFTRHDGYAESATDIVSYCVGHV
                     LEMVPPETINPAYATWSLDTLPLKLYPVQLQPKASVAKQANTLVKLIRRPDIKMVVHC
                     GDPDDEGQLLVDEVLEFAGNTAPVKRALINDNTAPAVKKAINSLRDNSDFRGMYLKAL
                     MRSAGDAIFGFSMSRCYTLKARDKGYKGTISVGRVQTPVLGMIVRRWRDNQAHSEAFY
                     YQLAGQFISGTDVVCARWQTSEYAPVDDKKRLTDKAWGEGLARALAGKPASVLAAATD
                     RGKTTAAPLPFNLLRLQVYMNRRHKLTAQQTLDITQKLKDSKYITYNRSDCSYLSDEQ
                     FNDAPQVVAALKTLDVFNGLTTDTAQKSAAFDSKKVTAHTAIIPTTNMPDLSRLTDKE
                     KAVYLTIAQFYLAQFVAKKRYDESIAEIKCGDEMFKVSARKITDAGFTTFLNDAEEDD
                     EEDENSTSFEAISRLQTGATLTCREVVISEKKTKPLPLFTEATLLAALVRVADFVADP
                     RIKKLLKEKDKDKKDEHGGIGTPATRAAIIETLKTRDYITVEKGKFIPTAAGLALIDA
                     LPDSVTQPDMTAVWAEKQAAVETGEMTIERFIDGLYAEVSRLVESADIAISATEKHPV
                     KTALNRLAVKCPSCGSELVMTPKTCACTGCQFKIWLEVRGKKLTEKQAEKLIGKGKTD
                     VIKGFKSKKNDDTYSMIVTLKNAQTGELGFEYPPRDPLKGLNVSMPKELKL"
     gene            complement(6951..7259)
                     /locus_tag="JN749_003240"
     CDS             complement(6951..7259)
                     /locus_tag="JN749_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369555.1"
                     /translation="MATKTGFIAPPWPHVRGDSVSAASLISRIEKEAHRGCGLHYEIY
                     QYRALCELHDVLTSLSVRDAETFMTVAADHGFSLSDNDFQESRMMYFETMDEIRRDQE
                     "
     gene            complement(7235..7570)
                     /locus_tag="JN749_003241"
     CDS             complement(7235..7570)
                     /locus_tag="JN749_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369556.1"
                     /translation="MINQTVSINNFSGLLKDEELPAGDLQTVLLVLSEISQKQDHFQA
                     NTSELVQLAAQHSPTFQALPAERQSFFRSVLSMPVFFYTVPLSDDEAAEWEKLNDKDA
                     LQWQQKPDL"
     gene            complement(7597..7914)
                     /locus_tag="JN749_003242"
     CDS             complement(7597..7914)
                     /locus_tag="JN749_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369557.1"
                     /translation="MKHTVITAALLCALTSPAFAADGQNADPNDPCAMVLCLAGKLDG
                     SSPAECDPMYKNFMSIKKKKKGSFLPDHTAAARQKKLSACPSADSAIVGKIISKFGRM
                     NGW"
     gene            complement(8005..8406)
                     /locus_tag="JN749_003243"
     CDS             complement(8005..8406)
                     /locus_tag="JN749_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369558.1"
                     /translation="MMLPKLTGLTAIVVLLAGCSSPPEPAAVDWSQPAQPVTTTRPVW
                     EPNRLIVASPVTEGHWSQVLTRFEPGGIYPAAQWYAVAHASRAVVNAPDGQRFFAAKT
                     WLREGGYKGVVEFRPRSGCLTCDTSEIVFYR"
     gene            complement(8393..9442)
                     /gene="virB11"
                     /locus_tag="JN749_003244"
     CDS             complement(8393..9442)
                     /gene="virB11"
                     /locus_tag="JN749_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HAY7369559.1"
                     /translation="MDDKLTDTPSEGNVALSHLRDLLFGDYLSREGLTEIAINRPGEL
                     HTKINGAWEMHASPVTLRQCSSFARALAVWNNDTIDETSPILSATLPTGERCQIVVPP
                     ACERNTISITLRKPDYFQRTHQSYIDAGFYDRVQGTEKVESKDGELSRIYLSGNIPLF
                     MEKCVEYGKTLFVVGETGSGKTTYMKMLLHYIPETLRLITIEDNPELKFYSQKNYVHL
                     FYPADAGEGAIVTSDRLVRAIYRMNPDRPLLAEVRGREAWDALKLIESGHEGFMSSLH
                     AGSPRECISGLIDRCYEHPACRGMPVDVLLRKVLHCVDVIVSVDVHGNVRRMSDVYFK
                     PLHMQAMKESFNDAA"
     gene            complement(9446..10642)
                     /locus_tag="JN749_003245"
     CDS             complement(9446..10642)
                     /locus_tag="JN749_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HAY7369560.1"
                     /translation="MMNEQEDIKTAAELEAEARERARAEIDGDKPAAGAVPPGQPQVV
                     QHAKRRSRRSLVLAAISLVLLVIIATGGDYLMAKLKGDGSGKSASHEPESHATSPARE
                     RNNLGRDLNPLGQLADTTGQEKTTPTDTAAPPQASPPPLTFDKVSALADSAPTTASSA
                     PKSTSSSNVSPAGSASAPGSATPAGQSQDNGLARITGVKRLGLDPDLYIPADRHIPCA
                     MMQRFVSDVGGRFNCLISEDIYSASNAVRLIPAGTEARGLYRTGTLKNGQGRLFLAIT
                     ELRTPEPGRLVIPMVDSQAVGALGENGVAGWIDNHWLERIGNTLLLGTVQDFAAAASG
                     SSPGKDRNTDYTENTRAATAEMAKTLLENSINIPPTMYLNQGDVIGLVTGADIDFSDV
                     YRLRMR"
     gene            complement(10639..>11241)
                     /locus_tag="JN749_003246"
     CDS             complement(10639..>11241)
                     /locus_tag="JN749_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAY7369561.1"
                     /translation="GKTVQKIFTPTQQDWQTNLFVTTTKRDYSLMLNVIDNDREAGNL
                     AFVVRFSYPDDTRRQNDAARLARQQELQAAQEKERIQFALKKSTAPRNWNYTRRVAAG
                     SESITPDFAYDDGRFTYLGFSPQKLIPSPTVIVGGSEQVITPTFSTEGNYRVMVLRSL
                     SPRFVLRYGSQVVGIENGSYGKVTVANGSTVSPDVKLESK"
BASE COUNT         2224 a         3202 c         3199 g         2616 t
ORIGIN      
        1 accatgacga tgaacctgca caccccgaca accgccccta aagctgcacc ggcgaccagc
       61 gtatccccgc tggagcagtc aggctcccca aaaacgaaat ttcctgaaat caaaaccgtc
      121 aaaacggcgg tggttaaact gatcagcgac atcgagggca acctcgatca gcgtctggta
      181 tatcgcgagg ccgtagaggg attgtgccag gaggcggcca accggataac ccagtgcatg
      241 cattcacgca gtacagggca gacgctacgt gatctccgcg agctgctgtc ggtgacgctg
      301 gacaccatta tcgtggtgga gtttgaaggg gagcagaccc gtctcaacta tggcggacgt
      361 tcagcagaag acgtgatcgc catgctgaaa gcgctgcatc ttgaaggacg caaagcctgg
      421 ctggaagtgg agtaaaaaaa aagccccgtc cgcagacggg gctgattcag tacagggcat
      481 tcagcgggat gccctcatcc cacaggcggt taaattctcg ctggtacgcc tccgccagct
      541 gcggcgcatc ctcgatcagc agcacgtttt ccgcgttgcg cgaaacggcg cttgccgtgt
      601 agttaaacga cccggtctgg acgtttttgc cgtcgatcac cataaacttg ttgtgcatga
      661 tggcgtaacg gccgttaagg tgcaccggca cgccctggtt tatcagatac gtcacggcgg
      721 tgtagcggtc gctgttcgcc ttttcgtcgg ccaccacgcg cacggccacg ccgcgccggt
      781 tagcaccggc gagcgccgtc gcgaccggct tactggtgaa gctgtaggcc gcgacgtcaa
      841 tgctctgcct ggcgttgttt atggcgccga gcacgatttc aagagcaggg cgcccgtcag
      901 acggcgaaaa acccgccgtc aggacgggcg cggcggtggc gaacgcgggc acgcagaggg
      961 cggcggccag caccagaagg gtggttttac gcatagttgt ccagctccag gtttttcagt
     1021 tcatcgaggg tgtaaaagcg gaaaacgtcg cggtgttttg cctccagttg cacatgctgg
     1081 tttttcacga tcacgctggc gatgctgtcg aaaataatct tcagggcgcg ctttttcgcc
     1141 tcgtccgggg cgacgtaaaa cgcgtaccgc cattttttat cgctgatagc cagcaggtgg
     1201 ctcgccatga tggactgata gcgcgccttt gttttgatac gtcgttcggt ttcaacggcg
     1261 atcagcctgc cgtccggcag gctgataagc ccgtcgggac ggtgtttgac gctgtactga
     1321 ctgaggaacg ttgaacggtc gccgttaatc caccctgtcg cgccgttctt ctccagcgta
     1381 aggcgcacta gctggttatc aaggtgatgg tcgagtgacc agccggtcag gcggtagggt
     1441 tcaaagcggg cgggcattat gttgtcgtcc gccgtgagga taacggccag cccgtcggtg
     1501 gtaatgcccc acagggatat tctcgacgcg cgggattcat gcacgtgctt ctgaatcagc
     1561 cccattttct ccaccttctc caacaaccgg tagagagggc ggtcagattt aaaatcaaaa
     1621 agcagcttta tgatttgaaa atcgctgtag gtttcctcct tgagaaaatt cagcagtacg
     1681 cgaatttttt cagtgctgcg tgcataacgt tcctgatacg acgaaataag catagaaact
     1741 ccttatttta taaatccagc aacgaatgta tttcgtcatt tggagcgggc tgtgatgcca
     1801 tcagccactc ctcgtcaggt tgcaccggcg gagtgggtat tggcgttgct gagggttttt
     1861 tggtggttgt tgcgctgacc ggaataatgt caggtgccgg aactgccgga gccgttttat
     1921 caaaatccag atccagcacc ggtttaactt ctgccaccgt agcgactgcg accggcggca
     1981 gggtaaacag ctccagtggg gcttttttaa ccttgagcgg tgagattaac gacgggaacg
     2041 cgagcgcgtt ctggctgaag atatagctga cgaacttcgg caggttctgg agcatattgc
     2101 tgtcgatgta atagctttcc gcctgccgaa gcatgcgctc gttatcgact ttttctgtca
     2161 gcaccgccgt ggtggtgact ttccggcttt catcatcgac taaaatcgtg cccgacatgc
     2221 gcgcaatcca ttcggcggtg tctggatcct gaagtttata taccagctta aacttgcagt
     2281 tttccacaac cgccccaacc acggcgtcgc cgtttaaatc cgccggacag tcgcgcaggt
     2341 cggcaattga ctggtgcgcc attaatatat ggacgccttt atcgcgtgcc gcgcctaacc
     2401 cttcgagcgc cggtttggac aggtgatatt tcacctcgtc cttaaagatg gcgatggggc
     2461 ggatattccc cgcgacgcgg tcgcgcaatt ccgcgagctg gaacaggcga actaaaatca
     2521 tgcgctgcgc ggtaatgatt ttagagttac gcatcgaacc gatgatatag cagcagccgc
     2581 ccttgtcgaa aatctccttc agcgcaaaac cgcctggcgc gttaatgctg ttgagcattg
     2641 ccagctcctc ggttttaccg tgaaaggctt taatcgtctc gccaatgccc tgaacataat
     2701 cgccgttata aatatcccgc acggttgcgc ccggattatc cttcacgaac tgcgccgccg
     2761 ttctcgccgc cttgcggtcg tcgatacggt aaaaatccgc cgcctcgcct ttttccgcca
     2821 gactgaaacc ggccacgaat aattcctcca gatggtcggc ggtgataccg tcgataaggt
     2881 tcatctggta ttcctgttta ttcaggtcga taagcacaaa gggttttccg gcgtccgcgc
     2941 acgctttttt atacaggtgc ggtgcccact cgtcgttttt ggggtcgaga acaaaaacgc
     3001 cctcgtcggc gaggatgctc tgataaagca gaataccggt tacgacgcct ttaccggcac
     3061 cagtggtgcc gataatgtcg gcgtgctggg tctgccattc ttttaacgga atatattgcg
     3121 gctcaccctc gcggctcagc ccgataaaaa tccccttatt cagatcgatg taatccatcg
     3181 gatcgtattc tgtggtgacg ggtaaaattt cttttacccg gcgaacgtcg gttctgcggt
     3241 cgcgctccat gctggttttt ttcaccatgc cgcatttgac cttatcaaat tcaggcgaga
     3301 tcaaacgccg taaaaccacg tgcagaataa aaccggcgac ggtaaacagt agcgccattg
     3361 tccaggtaag cagcggcgga ctggcgatat cttcccggcc ataaatgcgc tgtaaaaacc
     3421 agacggccac tggcgctatc gagccgaaaa caaaacagat catggaaagc agaattaaga
     3481 tcctttgcag aaacgtcggc ttttcctgac cgccgtgttt cgcagaaaat ataaaaggca
     3541 gcatgacgcc ggacagcagc gctaacgcca gttgatgcgc ctgcaaaaag gcgataaagg
     3601 ccaggacggc atttgctatc ggcgacagca aatgattcag tttgtcgaat atcacgatat
     3661 tctccttcac ttaaattcgg tcagcaccat cggtcagtca gaacggtcac aggttgtgat
     3721 cgttctgact gaccgattcc atgctgcaaa agaagtggcc gctaagcggc catgatcgca
     3781 ggaggtacac tcccgcgatc taacggcgtg cggcattacg cgcgggctgg acgcccttac
     3841 tgcatgtcgc atgggggtaa tgtcctggct gaacgcccgg ccaaaacccc caaggtcaaa
     3901 agaggccaca ccgtcgcgcg ctacgcgcga ccacccccta aaagacgcct gttttagccc
     3961 ttttcttcgc cttgttcgtt cctcacaaga cgaattaaag ggaaactagg ctttttaggg
     4021 ggtggtcgcg ccacgctacc ggcttttggc cggagactta ggcacgacgg tgtggggggc
     4081 aagcccccca aataaaccga cgcgtgggcg cgtctttttg ctcgctgggg cggcaaaatt
     4141 tattcggccc cccccggctt ttttcggctc acttggggtg agcgctcaaa aagtggaatc
     4201 ggcgacgcaa attagcaatt tcttcgaaat tgaatttact cgcctcctgc cacttcgcag
     4261 actcaggcgg cctgtcgatt agcaattttt tcaaaattga atcgccatgc tcgccagtct
     4321 tcgaagcccg aaaagccagg tcaaaaccga ccctgtgccg ttgttagtcg gtggtaataa
     4381 ggaaggattc aagaggtttt ccggcgtcaa gttcttgctg aattacaacc ggcacgcgtc
     4441 cgacgcctga ccatttttta tgcacgccgt tttcaaaata ctgatattta gctttgcgtt
     4501 ttgccgactt tttggcggga gttgtactaa ccagctctgt cggtgaaaag ccctccgatt
     4561 caatcagcgc cagcaattca ttgcgctttt cttcccgctg tcggcgtagc ttttcctgct
     4621 cgatatgctc tgcgcgtttt tcatcaagcg ctgctgaaat acgtgcactc agcatctcca
     4681 gaaaggcaaa atcactttca cgtaaaaact ttttcagcgc gcgctctgag gttaattcgt
     4741 cctggatacg gttcataatt tcagctcctt cggcatagat acgttcaatc ctttcagcgg
     4801 atcgcgtggt ggatattcaa aaccgagttc cccggtttgc gcgtttttca gcgtgacgat
     4861 catcgaatag gtgtcgtcgt ttttcttcga tttaaaacct ttaataacgt cggttttgcc
     4921 cttgccgatt aacttctcgg cctgtttttc cgtcagcttt ttgccgcgca cttccagcca
     4981 tattttgaac tgacagcccg tacaggcgca ggttttcggc gtcataacca gctcagaacc
     5041 gcacgacgga catttaaccg cgaggcggtt aagcgccgtt ttaacgggat gtttttccgt
     5101 cgcgctgatg gcaatatcgg cgctctcaac caggcgggaa acttccgcgt aaagaccatc
     5161 aataaagcgc tcgatggtca tttcgccggt ttcaaccgcc gcctgttttt cagcccatac
     5221 ggcggtcata tccggctgcg tcaccgagtc gggcagggca tcaattaacg ccagcccggc
     5281 ggcagttggg ataaatttgc ccttttccac ggtgatgtaa tcgcgggttt tcagcgtctc
     5341 aataatagcc gcgcgggtcg ccggtgtgcc gatgccgccg tgttcatctt ttttgtcttt
     5401 atccttctcc ttgagcaatt ttttaatgcg cggatcggcg acaaaatcgg ccacgcgaac
     5461 gagggcagcg agcagggtcg cctcggtaaa cagcggcagc ggtttagttt ttttctccga
     5521 aatcaccacc tcgcggcagg ttaatgtcgc cccggtttgc aggcggctga tggcctcaaa
     5581 cgaggtgctg ttttcgtcct cctcgtcgtc ttcttccgcg tcgttcagga acgtcgtaaa
     5641 acccgcatcg gtgattttac gcgccgagac tttaaacatc tcgtcaccgc atttaatctc
     5701 cgcgatcgac tcgtcatagc gctttttcgc cacgaactgc gcgagataaa actgcgcgat
     5761 ggtgagataa accgcctttt ccttgtcggt aaggcggctt aaatccggca tgttcgtcgt
     5821 cgggataatg gcggtgtggg cggtgacttt tttactgtca aacgccgcac ttttttgcgc
     5881 cgtgtcggtg gtaaggccgt taaaaacgtc gagcgttttt aaggccgcca ccacctgtgg
     5941 cgcgtcgttg aattgttcat cggacagata agaacagtca gagcggttgt aggtaatgta
     6001 tttactgtcc ttgagcttct gcgtgatatc gagcgtctgc tgcgcggtca gtttgtgcct
     6061 gcggttcata tacacctgga ggcgcagcag gttaaacggc agcggtgcgg ccgtggtttt
     6121 tccccggtcg gtggcggccg cgagcacgct ggccggtttt ccggccagag ccctcgcgag
     6181 tccttccccc caggccttat ccgtcagccg ctttttatcg tccaccggcg cgtattcgga
     6241 cgtctgccag cgggcgcaga caacgtccgt accgctgata aactgccctg cgagctggta
     6301 gtaaaacgcc tcgctgtgcg cctgattgtc gcgccaccgg cgcacaatca ttcccagcac
     6361 cggcgtctgg acgcggccaa ccgaaatcgt gcccttatag cccttatcgc gggctttgag
     6421 ggtgtaacag cggctcatac tgaagccaaa aatcgcgtct cctgcgctgc gcatcagggc
     6481 tttcaggtac atcccacgaa aatcgctgtt atcgcgcagg gagttgatgg cttttttcac
     6541 cgctggcgcg gtgttgtcgt taataagcgc ccgtttcacc ggcgcggtgt ttccggcgaa
     6601 ctccagcacc tcgtcaacca ggagctgccc ctcgtcgtcc gggtcgccgc agtggacaac
     6661 cattttgata tccgggcgcc gaatcagctt caccagggta ttagcctgct ttgccacgct
     6721 cgcttttggc tgaagctgta ccgggtacag cttcagcggc agggtgtcga gcgaccaggt
     6781 ggcgtaggcc gggttgatcg tctccggggg caccatttcc agcacgtggc cgacgcagta
     6841 gctcacgata tcggtcgccg attcggcata gccgtcgtgc cgggtgaagt tgccgcccag
     6901 cgctttcgcg atatcgctcg cgactgcggg tttttctgca atccacagtc tcattcttga
     6961 tctctcctga tttcatccat cgtttcgaag tacatcattc gactttcctg aaagtcgtta
     7021 tcagacagac taaagccatg atccgccgct actgtcatga acgtctcagc atcacggacg
     7081 ctgagactgg tcagaacatc gtgcaattcg cacagggcgc gatattggta gatttcgtaa
     7141 tgcaggccgc agccgcgatg cgcttctttc tcgatgcgag aaatcaggct cgcggcacta
     7201 acggaatcac cacgcacatg cggccacggc ggggctataa atccggtttt tgttgccatt
     7261 ggagtgcatc cttatcgttg agtttttccc attccgccgc ctcgtcgtcg ctcagcggaa
     7321 cggtgtaaaa gaagaccggc atactgagca cggagcggaa gaacgactga cgttctgctg
     7381 gtaatgcctg gaaggtcggc gagtgctgcg cggcgagctg gacgagttcg gacgtgttgg
     7441 cctgaaaatg atcctgtttc tggctgattt ccgacaaaac gagcagcacc gtctgcaaat
     7501 cacctgcagg aagctcctca tctttcagca gcccggaaaa gttattaata gaaacggttt
     7561 gattaatcac gtggtgtctc ctttacggct gccgttttac caaccgttca tacggccaaa
     7621 tttcgagatg atcttgccga caatggcgct gtcagcggac gggcaggcgc tcagtttttt
     7681 ctgccgcgct gcggcggtgt gatcgggtaa aaacgacccc ttttttttct ttttgatgct
     7741 catgaaattt ttgtacatcg ggtcacactc ggcgggactg ctgccgtcga gctttccggc
     7801 caggcacagc accatcgcgc acgggtcatt cgggtcggca ttctggccat cggcggcgaa
     7861 cgccggagaa gtaagggcgc acagcagcgc cgccgtgatt acggtgtgtt tcatgatgat
     7921 ttcctctgaa gggaagcagg gcgctaacgc ccgttggttg ttcaacgtcg gtgacggtct
     7981 gaaggactca gaccgtcacc gacgttatcg gtagaagacg atttctgagg tgtcgcaggt
     8041 caggcagccg gagcgggggc gaaactccac cacacccttg tagccgccct cgcggagcca
     8101 ggttttggcc gcaaagaagc gctggccgtc cggggcattt actaccgcgc gcgaggcgtg
     8161 cgcaaccgcg taccactgtg cggccgggta aatcccgccg ggttcaaaac gcgtaagcac
     8221 ctgcgaccag tgcccttccg tcaccggcga ggccacgata agacggttgg gttcccatac
     8281 cggccgggtg gtcgttaccg gctgcgccgg ttgtgaccag tcaacggccg caggctccgg
     8341 tggcgaagaa cagcccgcca ggaggacgac gatcgccgtc aggccggtca gtttaggcag
     8401 catcattgaa actctccttc atagcctgca tgtgcagggg tttaaaatag acgtcgctca
     8461 tgcggcgcac gttgccgtga acgtccacgc tcacaatgac gtcgacgcag tgcagtacct
     8521 tgcgcagcag cacgtcaacc ggcatgcccc ggcacgccgg atgctcgtag cagcggtcaa
     8581 tcagcccgga gatacactcg cgcgggctgc ccgcgtgcag ggaggacata aacccctcgt
     8641 gcccggactc aatcagcttg agtgcgtccc acgcctcgcg gccacgcacc tcggcgagca
     8701 gggggcggtc ggggttcata cggtaaatcg cgcgcaccag gcgatcagag gtgactattg
     8761 cgccctctcc ggcgtccgcc gggtagaaca gatgaacgta gtttttctgg ctgtaaaact
     8821 tcagctccgg gttgtcctcg atggtgataa ggcgcagcgt ctcgggaatg tagtgcagca
     8881 gcatcttcat gtaggtggtt ttcccagagc ctgtttcccc caccacaaag agcgttttgc
     8941 cgtactccac gcacttttcc ataaaaagcg ggatattccc ggacaggtat atgcggctca
     9001 gctccccgtc ttttgattcc accttttctg taccctgcac ccggtcataa aacccggcat
     9061 cgatatacga ctgatgggtg cgctggaagt agtccggctt acgcagggtg attgagatgg
     9121 tgttacgctc acaggccggg ggcaccacta tctggcagcg ttctcctgta gggagcgtgg
     9181 ccgagaggat aggcgacgtt tcatcaatgg tgtcgttgtt ccacacggcc agcgccctgg
     9241 caaaggacga gcactggcgc agcgtgaccg gcgaggcgtg catttcccac gccccgttga
     9301 tttttgtgtg cagctctccg gggcggttga tggcaatttc cgtcagcccc tcccggctca
     9361 ggtagtcccc gaacaacaga tcgcgcaggt gtgacagcgc gacgttgccc tctgacgggg
     9421 tatcagtcag tttatcgtcc atcgcttacc tcatgcgcag tctgtagacg tctgaaaaat
     9481 caatgtccgc ccccgtgacc aggccaatca cgtcaccctg gttgaggtac atcgtcggcg
     9541 ggatgttgat gctgttttcc aggagcgttt ttgccatttc cgccgtggcc gcgcgggtgt
     9601 tctccgtgta gtccgtgttg cgatccttgc ccggcgacga gccggaggcc gcagcggcaa
     9661 agtcctgtac cgtgccgagc aggagggtat tacctatccg ctccagccag tgattgtcta
     9721 tccagcccgc gacgccgttc tcgccaagcg cccccacggc ctggctgtca accatcggaa
     9781 taaccaggcg gccgggttcc ggcgtgcgca gctcggtgat ggcgaggaac agacgcccct
     9841 gcccgttttt cagcgtgccg gtgcggtaca ggccgcgcgc ctcagtgcct gccgggataa
     9901 ggcgcacggc gttgctggcg ctgtaaatgt cctcgctgat aaggcagtta aagcgcccgc
     9961 ccacgtccga tacaaagcgc tgcatcatcg cgcaggggat atgccggtcg gccggaatgt
    10021 acaaatccgg atcgaggccg agccgcttca cgccggtaat gcgcgccagc ccgttatcct
    10081 gcgactgccc ggcgggtgtt gcagagcctg gcgcggacgc gctgcccgca ggtgatacgt
    10141 tactgctgga cgtgcttttc ggggcgctgc ttgcagtggt tggggcgctg tctgccaggg
    10201 cgctgacctt gtcaaacgtc agggggggcg gtgatgcctg cggcggcgcg gcggtatcag
    10261 tcggagtagt cttctcctgg ccggttgtgt ctgccagctg tcccagcggg ttcagatccc
    10321 gtccgaggtt attgcgctcg cgggcggggg aggtggcgtg cgattccggt tcatggctcg
    10381 cgctttttcc gctgccgtca cccttcagtt tcgccatcag gtaatccccg ccggtggcga
    10441 taatgaccag taacaccagg ctgatggcgg ccagcacgag cgagcggcgg ctgcggcgct
    10501 tcgcgtgctg caccacctgt ggctgtccgg gtggcaccgc gcctgcggcg ggtttatcgc
    10561 cgtcaatctc tgcccgcgcc cgctcgcggg cttccgcctc cagttcggcg gcggttttaa
    10621 tgtcttcctg ttcgttcatc atttgctctc cagtttcaca tccggtgaga cggtgctgcc
    10681 gttcgccacg gtgactttcc cgtaactgcc gttttcaatc cccacgacct ggctgccgta
    10741 gcgcagcaca aagcgtggag aaagagagcg cagtaccatc acccggtagt taccctcggt
    10801 gctgaaggtc ggggtgatca cctgctcgct gccgccgacg atgacggtcg gcgacgggat
    10861 cagcttctgc ggtgaaaagc cgagataggt aaaccggccg tcgtcatacg caaaatccgg
    10921 ggtgatcgac tccgaacctg ccgccaccct gcgggtgtag ttccagttgc gcggcgcagt
    10981 ggatttcttc agggcaaact gaatgcgttc tttctcctgc gcggcctgaa gctcctgctg
    11041 acgggcgagg cgggctgcgt cgttctgcct gcgggtatcg tcggggtagc tgaagcgcac
    11101 cacaaaggcc agatttcccg cctcgcgatc gttatcgatc acgttcagca tcaggctgta
    11161 gtcgcgcttt gtggtggtca caaacaggtt ggtctgccag tcctgctgcg tgggggtgaa
    11221 aatcttctgg acggttttgc c
//
LOCUS       DADLTF010000130        11200 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.130, whole genome shotgun sequence.
ACCESSION   DADLTF010000130 DADLTF010000000
VERSION     DADLTF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.130"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            34..393
                     /locus_tag="JN749_003247"
     CDS             34..393
                     /locus_tag="JN749_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HAY7369562.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(513..692)
                     /locus_tag="JN749_003248"
     CDS             complement(513..692)
                     /locus_tag="JN749_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HAY7369563.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            766..2127
                     /gene="pabB"
                     /locus_tag="JN749_003249"
     CDS             766..2127
                     /gene="pabB"
                     /locus_tag="JN749_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HAY7369564.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2131..2709
                     /locus_tag="JN749_003250"
     CDS             2131..2709
                     /locus_tag="JN749_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HAY7369565.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2893..4257
                     /gene="sdaA"
                     /locus_tag="JN749_003251"
     CDS             2893..4257
                     /gene="sdaA"
                     /locus_tag="JN749_003251"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HAY7369566.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDNLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4388..5986
                     /locus_tag="JN749_003252"
     CDS             4388..5986
                     /locus_tag="JN749_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7369567.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="JN749_003253"
     CDS             complement(5990..7540)
                     /gene="yoaE"
                     /locus_tag="JN749_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HAY7369568.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENCDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            8003..8974
                     /gene="manX"
                     /locus_tag="JN749_003254"
     CDS             8003..8974
                     /gene="manX"
                     /locus_tag="JN749_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAY7369569.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFIPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9037..9837
                     /locus_tag="JN749_003255"
     CDS             9037..9837
                     /locus_tag="JN749_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAY7369570.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9850..10701
                     /locus_tag="JN749_003256"
     CDS             9850..10701
                     /locus_tag="JN749_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HAY7369571.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10756..>11200
                     /locus_tag="JN749_003257"
     CDS             10756..>11200
                     /locus_tag="JN749_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HAY7369572.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLL"
BASE COUNT         2749 a         2829 c         2827 g         2795 t
ORIGIN      
        1 cgtgccatat cgttataatc cctttgatat tagatgcaaa ttaaggtcat atatagcctt
       61 atcgataaca tggttaattt taaggataag aatatgcctg ctgtaataga taaagccctg
      121 gatttcattg gtgccatgga tgtatcagcg ccaacaccaa gttcgatgaa tgaaagcacg
      181 gcgaagggaa tctttaaata tttaaaagaa ctgggagtac ccgcgagtgc cgctgatatt
      241 acggcgcgag ctgaccagga aggctggaac cctgggttca cggaaaaaat ggttggatgg
      301 gcaaaaaaaa tggagacagg tgaacgctct gtgattaaaa atcctgaata cttttcaaca
      361 tatatgcagg aagaactaaa agcactggtc tgagttaaat ttatatcagc ataaatgggt
      421 caggacgctt ttaatcacat ataaaaagcg tcctttttct cacaatcaaa tgatcatcgg
      481 cttctataac gaaatcgata cggattagtg tgttactcgt cttcatcctc aaaacgcgcc
      541 acgattagct cgccggagtg gttggcacgc agctcttctg ccaccagcgc gattgcctgg
      601 ccgctactca tcccttgtgc catcagttcc tggatccgct cgacagcttt ttgctgctgt
      661 tcatgggtga gtgaaggtaa acctgcaaac attgttaact cctgctaaat tgttggcgct
      721 aattatttca tgctacccgg cacatagcca gtagagtcag gactgatgaa gacgttatct
      781 cccgctgtga ttactttacc ctggcgtcag gacgccgctg aattttattt ctcccgctta
      841 agccacctgc cgtgggcgat gcttttacac tccggctatg ccgatcatcc gtatagccgc
      901 tttgatattg tggtcgccga tccgatttgc actttaacca ctttaggtaa agaaaccgtt
      961 gttagtgaaa gcgaaaaacg cacaacgacc actgatgacc cgctacaggt gctccagcag
     1021 gtgctggatc gcgcagacat tcgcccaacg cataacgaag atttgccatt tcagggcggc
     1081 gcactggggt tgtttggcta cgatctgggc cgccgttttg agtcactgcc agaaattgcg
     1141 gaacaagata tcgttctgcc ggatatggca gtgggtatct acgattgggc gctcattgtc
     1201 gaccaccagc gtcatacagt ttctttgctg agtcataatg atgtcaatgc ccgtcgggcc
     1261 tggctggaaa gccagcaatt ctcgccgcag gaagatttca cgctcacttc cgactggcaa
     1321 tccaatatga cccacgagca gtacggcgaa aaatttcgcc aggtacagga atatctgcac
     1381 agcggtgatt gctatcaggt gaatctcgcc cagcgttttc atgcgaccta ttctggcgat
     1441 gaatggcagg cattccttca gcttaatcag gccaaccgcg cgccatttag cgctttttta
     1501 cgtcttgaac agggtgcaat tttaagcctt tcgccagagc ggtttattct ttgtgataat
     1561 agtgaaatcc agacccgccc gattaaaggc acgctaccac gcctgcccga tcctcaggaa
     1621 gatagcaaac aagcagaaaa actggcgaac tcagcgaaag atcgtgccga aaatctgatg
     1681 attgtcgatt taatgcgtaa tgatatcggt cgtgttgccg tagccggttc ggtaaaagta
     1741 ccagagctct tcgtggtgga acccttccct gccgtgcatc atctggtcag cactataacg
     1801 gcgcgactac cagaacagtt acacgccagc gatctgctgc gcgcagcttt tcctggtggc
     1861 tcaataaccg gggctccgaa agtacgggct atggaaatta tcgacgaact ggaaccgcag
     1921 cgacgtaatg cctggtgcgg cagcattggc tatttgagct tttgcggcaa catggatacc
     1981 agcattacta tccgcacgct gactgccatt aacggacaaa tatactgctc tgcgggcggt
     2041 ggaattgtcg ccgatagcca ggaagaagcg gaatatcagg aaacttttga taaagttaat
     2101 aagatattac gccaactgga gaagtaaaac gtggaatacc gtagcctgac gcttgatgat
     2161 tttttatctc gctttcaact tttgcgcccg caaattaacc gggaaaccct aaatcatcgt
     2221 caggctgccg tgttaatccc catcgtccgt cgaccacaac cggggttgtt gctgactcag
     2281 cgttcaattc atctgcgtaa acacgctgga caagtggcat tccctggagg tgcagtcgat
     2341 gacacggacg catcagttat cgccgccgcg ctgcgcgaag ctgaagaaga ggtcgctata
     2401 ccgccttccg ccgttgaagt tatcggcgtg ctgccgcccg tcgatagcgt aactggctat
     2461 caggtaaccc cagtggtcgg cattatcccg cccgatctgc cgtatcgcgc cagtgaagat
     2521 gaagtctcgg cggtgtttga aatgccgctc gcccaggcat tacatctggg tcgttatcac
     2581 cctttagata tctaccgccg tggcgattca catcgggtat ggctgtcctg gtacgaacag
     2641 tattttgtat ggggaatgac cgcaggcata attcgtgagc tggcgctgca aattggtgtg
     2701 aacccctgac tatacttatc tttacatcta caaaacacta cttgagacaa tcatcgcaat
     2761 attagttaaa tcgcggtttt tgattagttt aattcatgtg aatagttaag ccagtcgccg
     2821 cgttccctct tacactatgc gctgttatta gttcgttact ggaagtccag tcaccttgtc
     2881 aggagtatta tcgtgattag tctattcgac atgtttaagg tggggattgg tccctcatct
     2941 tcccataccg tagggcctat gaaggcgggt aaacagttcg tcgataatct ggtcgaaaaa
     3001 ggcttactgg atagcgttac tcgcgttgcc gtggacgttt atggttcact gtcgctgacg
     3061 ggtaaaggcc accacaccga tatcgccatt attatgggtc ttgcaggtaa cgaacctgcc
     3121 accgtggata tcgacagtat tcccggtttt attcgcgacg taaaagagcg cgaacgtctg
     3181 ctgctggcac agggacggca tgaagtggat ttcccgcgcg acaacgggat gcgttttcat
     3241 aacggcaacc tgccgctgca tgaaaacggt atgcaaatcc acgcctataa cggcgatgaa
     3301 gtcgtctaca gcaaaactta ttattccatc ggcggcggtt ttatcgtcga tgaagaacac
     3361 tttggtcagg atgctgccaa cgaagtgagc gtgccgtatc cgttcaaatc tgccaccgaa
     3421 ctgctcgcgt actgtaatga aaccggctat tcgctgtctg gtctcgctat gcagaacgaa
     3481 ctggcgctgc acagcaagaa agagatcgac gagtatttcg cgcatgtctg gcaaaccatg
     3541 caggcatgta tcgatcgcgg gatgaacacc gaaggtgtac tgccaggccc gctgcgcgtg
     3601 ccacgtcgtg cgtctgccct gcgccggatg ttagtttcca gcgataaact gtctaacgat
     3661 ccgatgaatg tcattgactg ggtaaacatg tttgcgctgg cagttaacga agaaaacgcc
     3721 gccggtggtc gtgtggtaac tgcgccaacc aacggtgcct gcggtatcgt tccggcagtg
     3781 ctggcttact atgaccactt tattgaatcg gtcagcccag acatctatac ccgttacttt
     3841 atggcagcgg gcgcgattgg tgcattgtat aaaatgaacg cctctatttc cggtgcggaa
     3901 gttggttgcc agggcgaagt gggtgttgcc tgttcaatgg ctgctgcggg tcttgcagaa
     3961 ctgctgggcg gtagcccgga acaggtttgc gtggcggcgg aaattggcat ggaacacaac
     4021 cttggtttaa cctgcgaccc ggttgcaggg caggttcagg tgccgtgcat tgagcgtaat
     4081 gccattgcct ctgtgaaggc gattaacgcc gcacggatgg ctctgcgccg caccagtgca
     4141 ccgcgcgtct cgctggataa ggtcatcgaa acgatgtacg aaaccggtaa ggacatgaac
     4201 gccaaatacc gcgaaacctc acgcggtggt ctggcaatca aagtccagtg tgactaatac
     4261 ttcttactcg cccatctgca acggatgggc gaatttatac ccgctttctc gtctgctgta
     4321 atattcccca ctacacttcc actgttgcgt caggcgtttg tcgccatacg cttacagggt
     4381 ggcccgcatg caaaaagcac agcggatcat taaaacctat cgccgtaatc gaatgattgt
     4441 ttgtacgatt tgcgcactcg ttacgctcgc ttcgaccctg agcgtgcgat ttatttcaca
     4501 gcgtaactta aatcaacaac gggtagtaca attcgccaat cacgctgtag aggaattaga
     4561 taaagtactg cttcccctac aggcaggtag cgaagtcttg cttccgctga ttggtctgcc
     4621 ctgctctgtc gcccatttgc cattacgtaa acaggcggca aaactccaaa ctgtgcgatc
     4681 cattggcctg gtgcaagacg gcacacttta ttgctccagc atttttggtt atcgcaatgt
     4741 gcccgtcgtg gacattctgg ctgaacttcc tgcaccgcaa ccacttttac gcctgacgat
     4801 cgaccgtgcc ctgattaaag gcagtccggt tttgattcaa tggacgcctg cagcaggcag
     4861 tagcaatgct ggggtcatag agatgattaa catcgactta ctgacggcaa tgctccttga
     4921 gccacaactg ccgcaaatca gtagcgccag cctgacggtg gacaaacggc atttgctcta
     4981 tggtaatggg ctggtagatt cccttccgca acctgaagac aatgaaaact atcaggtttc
     5041 ttcgcaaagc tttcctttta ccattaacgt aaatggtccg ggggctacgg cgctggcatg
     5101 gcactatctt ccaacacaat taccgctggc ggtgctgcta agtttactgg tgggctatat
     5161 cgcctggctg gcgaccgctt accggatgag cttttcccgc gaaattaatc tgggcctggc
     5221 gcaacatgag ttcgaattgt tctgtcagcc tttgcttaat gcgcgcagcc agcaatgtat
     5281 tggtgtagag attttgctgc gctggaataa tccgcgtcag ggctggattt caccggatgt
     5341 gtttattcct atcgcggaag aacatcattt aattgtgcca ctgacccgct atgtgatggc
     5401 agaaaccatt cgtcagcgcc atgttttccc gatgagtagt cagtttcatg ttggcattaa
     5461 cgtcgcaccc agccattttc gccgtggtgt gctgataaaa gatctcaatc agtactggtt
     5521 tagcgctcac ccgattcagc aactgatcct cgaaatcacc gaacgcgatg ccttactgga
     5581 tgttgattat cggattgccc gcgagctgca tcgtaaaaac gtcaaactgg cgattgatga
     5641 cttcggcacc ggcaacagct cgttttcctg gcttgaaaca ttacgtcctg acgtgctgaa
     5701 aattgataag tcatttaccg cagctatagg ttctgacgcg gttaactcga cggtgaccga
     5761 tatcatcatc gcgctggggc aaagactgaa tattgaactg gtggcggagg gcgtggaaac
     5821 gcaagaacag gcgaagtatt tgcgccgtca tggcgtgcat attttgcaag ggtatttgta
     5881 cgcacagccg atgccgctac gtgattttcc caaatggctg gcgggcagcc aaccgccgcc
     5941 cgcccggcat aatggacata tcacgcccat tatgtcgtta cgttaacgct tactcatctt
     6001 catcgtgcgc cggttgctct ttaacaatgc gaaccagatc aacacgataa tcattggctt
     6061 caaggatggt gatatgcagt ggccctacat caatcacatc gcccacacgg ggaatgtgac
     6121 catttgccga gatcacgagg cccgcgaccg tcgcgatatc atcgtcatcg gcaaggtgct
     6181 caacatcaag cgcctgctgc aaggcatgca aatctgtacc gccttttacc agccagccgt
     6241 taccatcggt aatgatttcc ggcgtttcgt cagcgtccgg gaattcaccc gcaatggctt
     6301 ccagcacatc cagcggcgtg accaagcctt gtaccacacc aaactcgttg gtcacgataa
     6361 caaagctccc gcgagcgcga cgcagcacgc ccagcaggtt gatcggatcg agggtttccg
     6421 ggacgataat cgccggagac gccgaagcaa tcgccgccac atcaacgccc tcttccagcg
     6481 ccaccagcag ttctttagca cgtacgatac cgatgatttc atccagttca ccgcgacata
     6541 ccgggaacag actgtgcggt gaagagagca gttgctcgcg gatttcatcg accccgagat
     6601 tagcgtcaac ccagcttatt tcaccgcgcg gcgtcatgat cccgcgcaga gaacgcgacg
     6661 ccagcgtcag tacgccgtta atcatgtacc gttcttcttc ggcaaatgca ccttccggga
     6721 tcggcatcgg gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac
     6781 gcaggatggc atcggcagta cgcgctcgca gcggcaaagt cgactggtga cgaataaagt
     6841 tgcgacgcgc aatctggtta aacacttcga taatgatcga gaagccaatc gcggcataca
     6901 ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta
     6961 acaggaagct cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg
     7021 gtttcgatgc cagcaacata accgccatcg caatcactac cgccgccatc atcaccggca
     7081 gatggttaac catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga
     7141 tgacaatctg tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat
     7201 gatcgcagtt ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc
     7261 ccccgaacaa cataatcaga tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg
     7321 gtttggtcag cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg
     7381 ccagtgataa ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa
     7441 gaatggcgat gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga
     7501 gtagccccgc ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga
     7561 atagctattc gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg
     7621 gcatagagtg ccagaaaggc aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg
     7681 atccatactg cgggctactg ccctatactc catggttgtt aaacgggagt taaacatatc
     7741 agagacgcct ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata
     7801 ttttaccttt cgaaatttct gataatcgaa agttaaatta cggatcttca tcacataaaa
     7861 taattttttc gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta
     7921 tctgaatcga ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta
     7981 acgataataa aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc
     8041 tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg
     8101 gatcgatttc attccaggtg aaaatgccga aacgctgatt gaaaaataca acgctcagtt
     8161 ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc
     8221 gttcaatgct gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt
     8281 taacattcca atgctcgtgg aaacgttaat ggcccgtgat gatgacccga gctttgatga
     8341 actggttgcg ctggcagtag aaacaggccg tgaaggcgtg aaagcactga aagccaaacc
     8401 ggttgaaaaa gccgcgccag cacccgctgc cgcagcacca aaagcggctc caactccggc
     8461 aaaaccaatg gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct
     8521 gattcacggt caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt
     8581 tgttagtgat gaagtggctg cggataccgt tcgtaagaca ctgctcaccc aggttgcacc
     8641 tccgggcgta acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc
     8701 gaaatatgct ggcgaacgcg taatgctgtt atttaccaac ccaacagatg tagagcgtct
     8761 cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg
     8821 taaaacccag gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaaaaa
     8881 actgaatgca cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa
     8941 aatgatggat ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcattcagtt
     9001 ttcacactta agtcttacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt
     9061 gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag
     9121 tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc
     9181 ggtattatta tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct
     9241 gcagttgcgc ctgacgccgc tctggcttct atcatttcta ccattctggt tatcgcaggt
     9301 catcagagca ttggtgcagg tatcgcactg gcaatccctc tggccgctgc gggccaggta
     9361 ctgaccatca tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct
     9421 gataacggca acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca
     9481 atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag
     9541 aacatgctga atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cgggggcatg
     9601 atcgtggtgg ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg
     9661 ttcttctacc tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt
     9721 gtgattggta ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta
     9781 gccggtgcgc ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag
     9841 gtgagcgaaa tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat
     9901 attcgtggcg tattcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg
     9961 caggcactgg gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac
    10021 aacgaagctc gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc
    10081 gtggctgcac cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca
    10141 gagatcgatg acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt
    10201 gtaggcgacc cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt
    10261 atcgcgatga gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg
    10321 cgtctggcaa cccgttacta cggcgtagct tatggttact ccaaaggtat cgatatcgtt
    10381 aaagatatgg gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg
    10441 tttgtcatgg gggcattggt taacaagtgg acacatgtca acatcccgct ggtggtttcg
    10501 accatcaccg ggcaggatgg acagacccgt gtcaccaccg tgcaaactat tcttgaccag
    10561 ttaatgccag gccttgtacc actgctgctg acatttgctt gtatgtggct gctgcgcaaa
    10621 aaagttaacc cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct
    10681 tgcggcctgc tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt
    10741 ttatctggag gattaatgac aatcacggac ctggtactga ttcttttcat cgccgcactc
    10801 ctggccttcg cgatctacga tcagttcatc atgccccgcc gtaacggccc caccctgctg
    10861 gcaattcctt tgctccgccg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc
    10921 attcttatct ataacaacgt cacgaatcat ggcgcgctaa taacaacatg gttattaagt
    10981 gcactggctc tgatgggttt ttatatattc tggatccgca ttccgaagat catctttaaa
    11041 caaaaaggtt ttttcttcgc caatgtctgg attgaatata gccgaatcaa agcgatgaac
    11101 ttgtcggaag atggcgtgtt ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt
    11161 cgaaatatcg acgatctgga aaaaatttat aagcttctcg
//
LOCUS       DADLTF010000131        11191 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.131, whole genome shotgun sequence.
ACCESSION   DADLTF010000131 DADLTF010000000
VERSION     DADLTF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.131"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JN749_003258"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="JN749_003258"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HAY7369573.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFSWRCDITSEQELSALADFAVSKLGKVDILVNNVGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /locus_tag="JN749_003259"
     CDS             complement(721..1749)
                     /locus_tag="JN749_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HAY7369574.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /locus_tag="JN749_003260"
     CDS             1924..3516
                     /locus_tag="JN749_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HAY7369575.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="JN749_003261"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="JN749_003261"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HAY7369576.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4801..5802
                     /gene="add"
                     /locus_tag="JN749_003262"
     CDS             4801..5802
                     /gene="add"
                     /locus_tag="JN749_003262"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAY7369577.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5838..6878)
                     /locus_tag="JN749_003263"
     CDS             complement(5838..6878)
                     /locus_tag="JN749_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAY7369578.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7121..7246
                     /gene="blr"
                     /locus_tag="JN749_003264"
     CDS             7121..7246
                     /gene="blr"
                     /locus_tag="JN749_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HAY7369579.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7519..7734
                     /gene="ydgT"
                     /locus_tag="JN749_003265"
     CDS             7519..7734
                     /gene="ydgT"
                     /locus_tag="JN749_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HAY7369580.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7820..8260
                     /locus_tag="JN749_003266"
     CDS             7820..8260
                     /locus_tag="JN749_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HAY7369581.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8337..8918
                     /gene="rsxA"
                     /locus_tag="JN749_003267"
     CDS             8337..8918
                     /gene="rsxA"
                     /locus_tag="JN749_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HAY7369582.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFATIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8918..9496
                     /gene="rsxB"
                     /locus_tag="JN749_003268"
     CDS             8918..9496
                     /gene="rsxB"
                     /locus_tag="JN749_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HAY7369583.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9489..>11191
                     /gene="rsxC"
                     /locus_tag="JN749_003269"
     CDS             9489..>11191
                     /gene="rsxC"
                     /locus_tag="JN749_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HAY7369584.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KA"
BASE COUNT         2602 a         2885 c         2816 g         2888 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgccattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccaccgc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cgacgttgtt aaccaggata tcaactttac ccagtttact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccagga
      421 aaatgcctga ccacccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 agaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttacc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctgc caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaagca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa tcgttgcgca atcgtggatt
     4741 tttaccctgc tttgttttta taatggtgcg cacttttata tccagaaaaa gagtgcgacc
     4801 atgattgata ccaccctgcc attaactgat atccatcgcc accttgatgg caacattcgt
     4861 ccccagacca ttcttgaact tggccgccag tataatatct cgcttcctgc acaatccctt
     4921 gaaacactga ttccccacgt tcaggtcatt gccaacgaac ccgatctggt gagctttctg
     4981 accaaacttg actggggcgt taaagttctc gcctctcttg atgcctgccg ccgcgtggca
     5041 tttgaaaaca ttgaagatgc agcccgtaac ggcctgcgct atgtcgagct gcgtttttca
     5101 ccaggctaca tggcaatggc acataagctg cctgtagcgg gtgttgtcga agcggtgatc
     5161 gatggcgtac gtgaaggttg ccgcaccttt ggtgtgcagg cgaagcttat cggcattatg
     5221 agccgaacct tcggcgaagc cgcctgtcag caagagctgg aggccttttt agcccaccgt
     5281 gaccagatta ccgcacttga tttagctggt gatgaacttg gattcccggg aagtctgttc
     5341 ctttctcact tcaaccgcgc gcgtgatgcg ggctggcata ttaccgtcca tgcaggcgaa
     5401 gctgccgggc cggaaagcat ctggcaggcg attcgtgaac tgggtgcgga gcgtattgga
     5461 catggcgtaa aagccattga agatcgggcg ctgatggatt ttctcgccga gcaacaaatt
     5521 ggtattgaat cctgtctgac ctccaatatt cagaccagca ccgtagcaga gctggctgca
     5581 catccgctga aaacgttcct tgagcatggc attcgtgcca gcattaacac tgacgatccc
     5641 ggcgtacagg gagtggatat cattcacgaa tataccgttg ccgcgccagc tgctgggtta
     5701 tcccgtgagc aaatccgcca ggcacagatt aatggtctgg aaatggcttt cctcagcgca
     5761 gaggaaaaac gcgcactgcg agaaaaagtc gccgcgaagt aacaaaaatg gatggtgcaa
     5821 attgcaccat ccatttttca cgcaaggcac aacgtcgcgc gatgtttggc ggattcgatg
     5881 cccagctcaa tcaactccat tacctggatt gcctggcttg ccggaaccgg attttcacca
     5941 tcgccattta acgcatcacg aatcgccgca tagtaagccg gataattccc aggcaccgtc
     6001 aacagcgttt cttcgacacg ttcctcacct tccacgcggg taagtacgcc atcacgcata
     6061 tcgtagcccc agtcttcctg cggcagacgc tcgccatttt tcagacgttc ttcctgtgga
     6121 tcgaggccat atttcacata actgcctcgg gatccatgca cgatataccg tgctgactca
     6181 gcggctgcca gcatggtacc gtgtaaaatg actcgccgct gtggatagga caagatggcg
     6241 tggaaataat cggtcgactg cgctccgggc cgtaactgtg ccaaatctac cgtcatgctg
     6301 accggtaaac caaatagcgt aatggcctga tcaagaagat gcggtgctaa atcgtaccag
     6361 ataccgctgc ctgggccgcc ctgttcacgc caacgatcgc gcacctgcgg acggaagcgg
     6421 tcaaaatgag actcaaagta agcaacttca cccagcacgc cttccgcgag caaacctttt
     6481 agcgtcaaga aatcgctatc ccaacgacgg ttatggaata cagacagcac acgccccagg
     6541 ctttttgcca gcgcatccag ctctcgcgct tgtgacagtg tcacggtaaa gggtttatca
     6601 acgaccacat gtttacccgc ctcaagcgcc gctttggcta acgggaaatg ggtatcgttg
     6661 ggtgtaggaa tgacaatcag gtctatgttg ggatcgttaa acagatgctt cggctcagag
     6721 acaaccgtaa ccgttggcca gtcggctttt acttttgttt catcactgct ggagattacc
     6781 gccagttcca gcccgggcgt gcccgcaatc aggggcgcat ggaaggtttt gctcgcataa
     6841 ccatacccaa tcaacccaac acggatgttg tcgctcatgt cattgcctct cattgaagta
     6901 tgatggctat ttgacactat cctttaccca cgctcaacag tttaataacc tgccagcaat
     6961 aagggatgtt gtttaactta agtcaaaaaa atagcgaatt ttccaacgac aaaagctaaa
     7021 tatcgcaaaa acctcagtaa aaatcttgct ggagctatta ttgctaagta acatttaccc
     7081 cctgaagtta atggatcaat caagagagat gtgggctgta atgaatcgtc ttattgaatt
     7141 aacaggttgg atcgttcttg tcgtttcagt cattcttctt ggcgtggcga gtcacattga
     7201 caactatcag ccacctgaac agagtgcttc ggtacaacac aagtaagctc tgcacttgtg
     7261 gagcgacatg ctgcccgtcc gggtgcatgt tttcacttgt cggatattaa accaggaatt
     7321 tattatcttg ttcgatgttg ttggtgattg tcagggatag taaagcgcgt tactcttttc
     7381 agagcagttt ttcatccctg aatatttgcc atcagcgcat tcttattatt gtaataacac
     7441 aagcgttcac ttttggtgaa attttatcat cactaataat acctacccct ttggtattaa
     7501 ttattttatg gaccttttat gactgttcag gactacttat taaaatttcg caaaatcagt
     7561 tcactcgaaa gtctggaaaa actctacgac catcttaatt acaccctgac ggacgatcag
     7621 gaactgatca atatgtatcg tgctgccgat caccgtcgcg cagagctggt ttctggcggg
     7681 cgtttgtttg acctcggcca ggtaccgaag tccgtctggc actatgtcca ataaagaaag
     7741 tagcgatcgg ttataaaagc cttataataa cgcccctaaa attagatttg cctgtttgcg
     7801 tcgctattct ggagaaccta tgaccaccac aacgccacaa cgtattggag gctggttgct
     7861 tggcccgttg gcctggcttt tagtcgctct gttgagtacg acgctggcgt tgttgctgta
     7921 taccgctgcg ttatcttctc cgcaaacatt tcaaacgctc ggcggacaag cactgacgac
     7981 acaaatctta tggggcgttt cttttattac cgctatcgcc atgtggtatt acacactgtg
     8041 gttgaccatc gctttcttta aacgtcggcg ttgtgttcct aaacactata ttatctggct
     8101 gcttatttcc gtattgctgg cagtaaaagc attcgctttt tcacccgtgg aagttggcat
     8161 agccgttcgc cagttgctgt ttactttgtt ggcaaccgca ttgatcgtcc cctatttcaa
     8221 acgctcgtcg cgggtaaaag cgacgtttgt gaatccgtaa taaccttaca gttaacctgt
     8281 tgtcgcctgc tctggattaa cggataatag gcggcttttt tatttcaggc cgaaaaatga
     8341 ctgactacct gttactgttt gtcggaactg tactggtcaa taactttgta ctggtcaagt
     8401 ttctcggtct ctgtccgttt atgggggttt ccaaaaaact ggaaaccgcg atgggcatgg
     8461 ggctggcaac aacgtttgtg atgacgctga cgtctatttg cgcctggctt atcgatacgt
     8521 ggattttgat cccacttaat ctgatttacc tacgcaccct ggcatttatt ctggtgattg
     8581 ctgtggtcgt gcagttcacc gagatggtgg tgcgcaaaac cagcccggtg ctttaccgtc
     8641 tgctggggat ttttttgccg cttatcacca ccaactgtgc agtgctcggc gtggcgttgc
     8701 tgaatatcaa tctcgggcac aatttcttgc agtcggcgct gtacggtttt tccgccgctg
     8761 tcggtttctc gctggtgatg gtgctcttcg ccaccatccg cgaacgcctt gctgtggctg
     8821 atgtcccggc accttttcgc ggtaatgcca ttgcgttaat taccgcaggt cttatgtctc
     8881 tggcctttat gggctttagt ggtttggtga agttgtaatg aatgctatct ggattgccgt
     8941 tgccgccgtg agcctgctgg gcctggcgtt tggcgccatt ctgggttatg cctcccgccg
     9001 ttttgcggtg gaagacgatc cggtcgttga gaaaattgac gaaatcttac cgcagagcca
     9061 gtgtggtcag tgcggttatc ccggctgtcg cccctacgcg gaagccatca gctgtaacgg
     9121 tgaaaaaatc aaccgttgcg ccccaggtgg cgaagctgtg atgctaaaaa ttgccgagtt
     9181 gcttaatgtc gagccgcagc cgctggatgg cgaagcgcaa gagctaacgc ctgcgcggat
     9241 ggtggcggtt attgatgaaa ataactgtat tggctgcact aaatgtattc aggcttgtcc
     9301 ggtagacgcc atcgttggcg ctacccgtgc catgcatacg gtaatgagtg atctctgtac
     9361 gggctgcaat ttatgtgttg atccgtgccc gacgcactgc atctcgttgc aaccggtcgc
     9421 agaaacacct gactcctgga aatgggatct gaacaccatt cccgtgcgta tcattcccgt
     9481 ggaacaccat gcttaagtta ttctctgcat tcagaaaaaa taaaatctgg gatttcaacg
     9541 gcggcatcca tccaccggag atgaaaaccc agtccaacgg tacccccctg cgccaggtac
     9601 cactggcgca gcgttttgtt attccactga aacagcatat tggcgctgaa ggtgagttgt
     9661 gcgttagcgt cggcgataaa gtattgcgcg gccagccgct tacccgtggt cgcggcaaaa
     9721 tgctgcctgt tcacgcgccc acctcgggta ccgttacggc tattgcgccc cactctacgg
     9781 ctcatccttc agctttagct gaattaagcg tgattattga tgccgatggt gaagactgct
     9841 ggatcccgcg cgacggctgg gtcgattatc gctctcgcag tcgcgaagag ttaatcgagc
     9901 gcatacatca gtttggtgtt gccgggctgg gcggtgccgg attcccgaca ggcgttaaat
     9961 tgcagggtgg tggagataag attgaaacgt tgattatcaa cgctgctgag tgcgagccgt
    10021 acattaccgc cgatgaccgt ttgatgcagg attgcgcggc tcaggtcgta gagggtattc
    10081 gcattcttgc gcatattctg cagccacgcg aaattcttat cggcattgaa gataacaaac
    10141 cgcaggcgat ttccatgctg cgcgcggtgc tggcggactc taacgatatt tctctgcggg
    10201 tgattccaac caaatatcct tctggcggtg ctaaacaatt aacctacatt ctgaccggga
    10261 agcaggttcc acatggcggg cgttcatccg atatcggcgt attaatgcaa aacgtcggca
    10321 ctgcttatgc agtgaaacgt gccgttattg atggcgagcc gattaccgag cgtgttgtaa
    10381 ccctgactgg cgaagcaatc gctcgcccgg gcaacgtctg ggcacggctg ggaacgccag
    10441 tgcgtcattt attgaatgat gccggattct gcccctctgc cgatcaaatg gtgattatgg
    10501 gtggcccgct aatgggcttt accttgccat ggctggatgt cccggtcgta aagattacca
    10561 actgtctgtt ggctccctct gccaatgaac ttggcgaacc acaggaagaa caaagctgca
    10621 tccggtgtag cgcctgtgct gacgcctgcc ctgtggatct tttgccgcaa cagttgtact
    10681 ggttcagcaa aggtcagcaa cacgataaag ctaccacgca taacattgcc gactgcattg
    10741 aatgtggggc ttgcgcgtgg gtttgcccga gcaatattcc cttggtgcaa tatttccgtc
    10801 aggaaaaagc tgaaattgcg gctattcgtc aggaagaaaa gcgcgccgca gaagccaaag
    10861 cgcgtttcga agcgcgccag gctcgtctgg agcgcgaaaa agcggctcgc cttgaacgac
    10921 ataagagcgc agccgttcaa cctgcagcca aagataaaga tgcgattgct gccgctctgg
    10981 cgcgggtgaa agagaaacag gcccaggcta cacagcctat tgtgattaaa gcgggcgaac
    11041 gcccggataa cagtgcaatt attgcagcac gggaagcccg taaagcgcaa gccagagcga
    11101 aacaggcaga actggagcaa actaacgacg cagcaaccgt tgctgaacca cgtaaaactg
    11161 ccgttgaagc agctatcgcc cgctccaaag c
//
LOCUS       DADLTF010000132        10876 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.132, whole genome shotgun sequence.
ACCESSION   DADLTF010000132 DADLTF010000000
VERSION     DADLTF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.132"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            126..1823
                     /locus_tag="JN749_003270"
     CDS             126..1823
                     /locus_tag="JN749_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAY7369585.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="JN749_003271"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="JN749_003271"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HAY7369586.1"
                     /translation="MKTAVITKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="JN749_003272"
     CDS             3113..3397
                     /locus_tag="JN749_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HAY7369587.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="JN749_003273"
     CDS             complement(3625..4278)
                     /gene="fdnI"
                     /locus_tag="JN749_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HAY7369588.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4271..5155)
                     /gene="fdxH"
                     /locus_tag="JN749_003274"
     CDS             complement(4271..5155)
                     /gene="fdxH"
                     /locus_tag="JN749_003274"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HAY7369589.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="JN749_003275"
     CDS             complement(5168..8215)
                     /gene="fdnG"
                     /locus_tag="JN749_003275"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7628..7630),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HAY7369590.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8447..9328
                     /gene="yddG"
                     /locus_tag="JN749_003276"
     CDS             8447..9328
                     /gene="yddG"
                     /locus_tag="JN749_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HAY7369591.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9587..10687
                     /gene="ompC"
                     /locus_tag="JN749_003277"
     CDS             9587..10687
                     /gene="ompC"
                     /locus_tag="JN749_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HAY7369592.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2674 a         2787 c         2688 g         2727 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacacttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tattacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 atttgaaact gatgacaaac gcaacattgc ctgatgcgct acgcttatca ggcctacacg
     3481 ttcacttcaa tctattgtat ttgaagggtt ttcatgccgg ataaggcatt cacgacgcat
     3541 ccgggatgaa caaagcccac gttgtctaca atatgaaaat ggcgcctttt acagcgccat
     3601 tttcagctta aagttcaaaa gagattatat cccttcttca ctctcttttt tcgcttctgc
     3661 cttctcgatt tcacgatacc agcgcggatg gtgtttcttc gcccagcgac ggctcacctt
     3721 cccttcgatc atccctttaa tcgacccttt cacccagaat gccatataca tatggatcag
     3781 gatggcgtgg atcaggatga tacccgcagc cgcgtggatc agcaggctgt agcgaacaac
     3841 ctgcatcggg aagtactgcg caaagtacgg acgccagata atcaccccgg tcaccagcag
     3901 cacgaaaatc atgctcatga tcgaccagaa catcatcttt tgcccggcgt tgtacttacc
     3961 aacatccgcc actttgtgct cattgccttt taatacttcg acaatgttca acagccacgg
     4021 aatatctttc ttatccggga tgttgtgatg cacaaaacgc acaaacataa acatcagtgc
     4081 gacgaaaatc gcaatgccga agaacgggtg caaaatgcgt cccatctgcg gcgtaccgaa
     4141 ggtttgcgtc agccattgca gcgtcgggaa gaagaacgaa atcccggaca gcgccaccag
     4201 gaagaagcaa atcaccacgg tccagtgaca ggcgcgatca ataaatttgg tgcgcacaat
     4261 cattttcgac ttactcatga tgatcctcct cgtcatcgtc cacttcctta ttcgggccaa
     4321 taccgatgta gtggaaaatc aacccggcaa aagtggcaat aaagccagcc gctgccagcg
     4381 gtttcaacgc gcctttccac aggcttaccg aggtgtcgat cttcggatct ttcggcagac
     4441 cgtgatacag ctccggctga tcggcgtgat gcagcacgta cataacgtgc gtaccaccga
     4501 ccccttccgg gttgtagacg ccagcatgtt cgtaaccacg cgctttcagt ttcgccacgc
     4561 gctgttccgc cagctccagc atctccttct tggtgccgaa gtggatagcc ccggtcggac
     4621 aggttttcac acaagccggt tcctggccga cgctgacgcg atcgacgcag agcgtgcatt
     4681 tatatacccg gttatcctct ttgttgaggc gcggaatatt aaacggacac ccggcaatgc
     4741 agtaaccaca gccgatgcag ttttccgact ggaaatcgac aatcccgtta gcgtactgaa
     4801 tgattgcacc agcagacggg cacgccttca ggcagcccgg atcttcacag tgcatacagc
     4861 cgtctttacg gatcagccac tccagcttgc cattctgttc ggtttcgcta aagcgcatca
     4921 ccgtccagga cttggcgctc agatcggcgg ggttatcgta aacaccgacg cagtgcccca
     4981 cttcatcacg gatgtcgttc cactccgaac acgccacctg acaggcttta cagccgatac
     5041 aggtggaaac gtcgataagt ttcgcgactt ctgctttgta atcacgcacc tgagaaggcg
     5101 gcgtgatgga gttagttgcg gaccttttga taatatcctg cgtttccata gccatctgtt
     5161 cgccccctta cgccttctcg atgttgacta agaacgcttt atattccggc gtttgcgagt
     5221 ttgcatcacc gacattcggc gtcagagtgt tagcgatata acctttacgc gcgacaccct
     5281 caaagcccca gtggattgga atacccaccg tttcaacctg ctgaccgttt acattcagcg
     5341 gtttcagacg gcgcgttacc actgccaccg cgcggataaa gcctcgcttg ctggagacag
     5401 tgacacgatc gccattatta atgcctttcg ccgccgcctg cgtttcgctg atttccacaa
     5461 actgttccgg ctgagcaatt gcgttgagca acgcgtgctt ggtccaggtg tggaagtgct
     5521 cggtcagacg ataggtcgta cccacatacg ggaactgctc ttttttaccc atccgcagcg
     5581 cgtcttgttc atacagacga acaaccgggt tagacaccac gttcggatgc agcgggttag
     5641 tgcccagcgg cgtttcaatc ggctcgtagt gttccgggaa cggaccttcc gccattttgt
     5701 tgatggcaaa caggcgtccc atcccttccg gctgcataat aaacggcccg gttggcgtac
     5761 ccggtgcggc attgccgaag tccggaatat cgttacccgt ccacttgctg ccgttccact
     5821 ggatcagcat ccgtttcgga tcccacggtt taccgttgat atccgccgaa gcacggttgt
     5881 agagtacgcg acggttgagc ggccacgccc aggcccatcc cagcgtattc cccagaccgg
     5941 acgggtcgga gttatcgcgg tttgccatct ggttaccctg ctctgtccag ctaccggtgt
     6001 agatccagca agaagatgcg gttgtaccgt catcacgcag atgcgcaaag ctactcagca
     6061 actgaccttt cttcgcaatc agcacgccat tagcatcata gagatcttcc aacgcatagc
     6121 cgttgttctc tttagctact tcgtcagatt gcggttcgtg cggctgcttg tagttccagc
     6181 tcatcttcat cagcggttct acgcctttac caccttcggc ctggtacaac tcgcgcagat
     6241 gatggtagat acccgccaga atttcgccgt cgttacgcgc ttcgcccggc gcgtcctgac
     6301 ctttccagtg ccactgcaac cagcgaccgg agttagcgat agaaccatcc tcttcagcaa
     6361 agcaggtcga aggcagacgg aatacttcag tctgaataga cgccggatcg acatcgttcg
     6421 actcgccgtg gttctgccag aaggtagagg tttcagtcac cagcggatcg ataaccacca
     6481 tgtacttcag tttgctcagg cagctcacca ctttgttttt atccgggaag gacgcaaccg
     6541 ggttaaagcc ctggcagaaa taaccggtga ctttgccttc atccatcatg ttgaaatact
     6601 tgatgacgtc gtaggtctgg tcccacttcg gcagccagtc atagccccag ttgttctctt
     6661 tctgcgcggc atcgccatag aaagatttca tcaggctaac gaagaacttc ggatagttgc
     6721 tccagtagtt cacctgatca gccagcgtcg ctttcggcgt gttcgcttcc agatacgact
     6781 gcaaatcaac ctgtttttct gacggcagcg tcagataacc tggcaggctg gtagagagca
     6841 gacctaagtc agtcagcccc tgaatgttgg agtgaccacg caatgcgttc acgccgccac
     6901 cggccatacc catgttaccg agcagcaact ggatcatcgc catagtacgg atgttctgcg
     6961 cacccacggt gtgctgcgtc cagcccagcg cgtacaggaa ggtggttgtg cgatccggtg
     7021 cgctggtgga ggccagcact tcacacactt tcaggaagtc ggcttttggc gtaccacaga
     7081 tgttttcaac aacgtccggc gtgtagcggg aaacgtgcgc tttcagcagg ttccacacac
     7141 agcgcggatg agtcagtgtt tcatcgcgtt tcgcatagcc gttttcatcg aactgatagt
     7201 tccaggacga tttgtcgtac tggcgttttt cagcgtcata accgctgaac aggccatctt
     7261 cgaaagcaaa atcatcacgc accagtaggc tggcgttggt gtaatgttta acgtattcgg
     7321 cgttgatttt gttgttttcg atcaggtagc gcaaaacgcc agacaggaac gtaatgtccg
     7381 taccggaacg aataggtgcg taaatatccg ccacagaagc ggtacgcgta aaacggggat
     7441 cgacaacgat caaggttgca tcgttgttgt ttttcgcttc catcgcccag cggaaaccga
     7501 cgggatgcgc ttcagcagcg ttaccgccca ttaccattac gacgttagcg tttttgatat
     7561 ccacccagtg gttggtcata gcacctcgac caaatgttgg agcaagactt gctaccgttg
     7621 gtccgtgtca gacgcgcgcc tggttgtcta ccgccagcat cccgagggag cgggcaaatt
     7681 tctgggtcag catcccggtt tcgttgctgg caccggaggc acacagcata ccggtagaaa
     7741 gccaacggtt taccgttacg ccctgctcgt tcttttcaat aaagttagcg tcacggtcag
     7801 ctttcatcag cttcgcaata cgggagaatg cttcttccca gctaatgcgc tgccatttgt
     7861 cagaacctgg cgcacgatat tccgggtagc gcagacggtt ttcactgttg acgtaatcca
     7921 gcaaaccggc ccctttcggg cacagcgcac cacggcttac cggatgatcc gggtcacctt
     7981 caatgtgata aatcgcttct ctggcgtttt ttgcgccatc acccaggcta tacatcaata
     8041 gcccgcaacc tacggaacag tatgtgcagg tgttacggat ctctttagcg cgtaataatt
     8101 tgtagtttcg cgcctgagcc agtgcttgct tcggggcaaa gcccaatgcc gctaccgttg
     8161 ttccagccat acctcccgcg cagattttaa aaaattgtct gcgactgacg tccattgctt
     8221 tcctcttttt tcaggggtat tactgcgcga ggaaactaac agtaaaattc tgaatctttt
     8281 tgcgcgaaat caaaaagaac acgtgtttgc cacaaaataa tccgcctgcg caaattgacc
     8341 tacctcaata gcggtagaaa aacgcaccac tgcctgacag gccagttaaa aaaatgctat
     8401 aaaattcagc ttaattttta acggcaagag agacaaaaca gcgagcatga cacgacaaaa
     8461 agcaacgctc atagggctaa tagcgatcgt cctgtggagc acgatggtag gattgattcg
     8521 cggtgtcagc gaggggctcg gcccggtcgg cggcgcagct gctatctatt cattaagcgg
     8581 gctgctgtta atcttcacgg ttggatttcc gcgtattcgg cagattccga aaggctattt
     8641 actcgccggg agtctgttat tcgtcagcta tgaaatctgt ctggcgcttt ccttagggta
     8701 tgcggcgacc cgtcatcagg cgattgaagt gggtatggtg aactatctgt ggcccagcct
     8761 gacaattctc tttgccattc tgtttaatgg tcagaaaacc aactggctga ttgtaccagg
     8821 attattatta gccctggtcg gcgtctgctg ggtgttaggc ggtgagaatg ggttacatta
     8881 tgatgaaatc atcaataata tcaccaccag cccattgagt tatttcctgg cgtttattgg
     8941 tgcgtttatc tgggcagcct attgcacagt aacgaataaa tacgcacgcg gatttaacgg
     9001 aattaccgtt tttgtcctgc taacgggagc atgtctgtgg atttactatt ttctcacgcc
     9061 acaaccagaa atggtattta gcacgcccgt catgattaaa ctcatctctg cggcatttac
     9121 cttaggattt gcttatgctg catggaatgt cggtatattg catggcaatg tcaccataat
     9181 ggcggtaggc tcgtatttta cgcctgtact ttcctcagcg cttgcagccg tgctgctcag
     9241 cgccccgctg tcgttctcgt tctggcaagg cgcgctaatg gtctgcggcg gttccctgct
     9301 ctgctggctg gcgacacgtc gtggttaaaa taattgccgg gtctacggcc cggcagttaa
     9361 tcagataaat tccgacaata ttaaaaaata aatttcaaat accagtaatt caccttatat
     9421 ctcatttcga cttcgatcac atatcgataa ataatattat ttcatctatt tttctcattg
     9481 tttttaatca ccattctatt ttaaaaacag tatgtcattt ataaatgacg ccgtttaaaa
     9541 ttcgtttaga aaatacgttt aacgataatt aaaaggattc tgttaaatga aattaaaaat
     9601 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
     9661 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
     9721 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
     9781 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
     9841 cgatgaagcc aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat
     9901 tagtgaattt ggctctctgg actatggtcg taactacggt gtcggctacg acattggttc
     9961 atggaccgat atgttgccag aatttggtgg cgatacctgg agtcagaaag atgtcttcat
    10021 gacataccgt accaccggtc tagcaaccta tcgcaactac gatttctttg gcttaattga
    10081 agggctgaac tttgccgcgc aatatcaagg caaaaatgaa cgtactgaca acagtcatct
    10141 ttatggtgct gactacacgc gtgccaacgg tgacggtttc ggtatctcct caacttatgt
    10201 ttatgatggc tttggtatcg gtgcggtata taccaaatcc gatcggacaa atgcgcagga
    10261 aagagccgct gctaatcctc tcaatgcctc cggtaagaat gcagaactat gggccacagg
    10321 tataaagtat gatgccaaca acatctactt tgcagctaat tacgctgaaa cattaaacat
    10381 gaccacctat ggggatggtt atatctctaa caaagcacaa agttttgaag tggtggcgca
    10441 atatcaattc gacttcggct tgcgcccatc actcgcttac ctgaaatcga aaggcagaga
    10501 tctgggccgc tacgccgacc aggacatgat tgagtatatc gacgttggtg cgacgtattt
    10561 cttcaacaaa aatatgtcga cctatgttga ttataaaatc aacctgattg atgaaagcga
    10621 ctttacccgt gccgtagata ttcgcaccga taacatcgtc gctacgggca ttacctatca
    10681 gttctaacgt cctctcctga ccagcccttc tctgggctgg tttttgattt aagtgctgct
    10741 gtatgactcc ttaagtaaaa atgagcaatt tttgatattt ctggtcattc acctcaatta
    10801 atattattcc atatcctccg gggacatctt actataatat ttattgatga gtgattatga
    10861 ccaatattaa tacagc
//
LOCUS       DADLTF010000133        10809 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.133, whole genome shotgun sequence.
ACCESSION   DADLTF010000133 DADLTF010000000
VERSION     DADLTF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.133"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            87..1172
                     /locus_tag="JN749_003278"
     CDS             87..1172
                     /locus_tag="JN749_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7369593.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1243..1851
                     /locus_tag="JN749_003279"
     CDS             1243..1851
                     /locus_tag="JN749_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HAY7369594.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1949..3340
                     /gene="selA"
                     /locus_tag="JN749_003280"
     CDS             1949..3340
                     /gene="selA"
                     /locus_tag="JN749_003280"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HAY7369595.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVTHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3337..5181
                     /gene="selB"
                     /locus_tag="JN749_003281"
     CDS             3337..5181
                     /gene="selB"
                     /locus_tag="JN749_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HAY7369596.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
     gene            5371..6522
                     /gene="yiaY"
                     /locus_tag="JN749_003282"
     CDS             5371..6522
                     /gene="yiaY"
                     /locus_tag="JN749_003282"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HAY7369597.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            6687..8225
                     /locus_tag="JN749_003283"
                     /pseudo
     CDS             6687..8225
                     /locus_tag="JN749_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(8444..8599)
                     /locus_tag="JN749_003284"
     CDS             complement(8444..8599)
                     /locus_tag="JN749_003284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369598.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRP
                     LTIETRD"
     gene            8770..9093
                     /locus_tag="JN749_003285"
     CDS             8770..9093
                     /locus_tag="JN749_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7369599.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            9099..10235
                     /locus_tag="JN749_003286"
     CDS             9099..10235
                     /locus_tag="JN749_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7369600.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(10232..>10809)
                     /locus_tag="JN749_003287"
     CDS             complement(10232..>10809)
                     /locus_tag="JN749_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7369601.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         2577 a         2656 c         2975 g         2601 t
ORIGIN      
        1 gcactttctg accgaatttc ccggctggag catcaactcg ccgccgagaa aaagactgac
       61 tattccacgt tcccggagat ctaattatgg atctattgat tgttttaact tacgtggcgc
      121 tggcgacggc ggcgctggga ggcgtgtttc tggtgagtgg tttgattttg ttgatgaact
      181 acaaccaccc ttacactttt accgcgcaaa aggcagtgat agcgatccct atcacgccac
      241 aggtgacggg aattgttact gaagtcactg acaagaataa tcagcttatt caaaagggcg
      301 aggtgctttt taagctcgac ccggttcgtt accaggcgcg agttgacaga cttcaggctg
      361 acctgatgac ggcgacgcat aatataaaga cgctgcgtgc gcagctcact gaagcgcagg
      421 ccaacaccac ccaggtttca gcggagcgcg accgtctgtt taaaaattat caacgttacc
      481 tgaaaggcag ccaggcggcg gtgaatccgt tctcggaacg tgacatcgac gatgcgcggc
      541 aaaatttcct cgcgcaggat gcgttagtga aaggctcggt ggcggagcag gcgcagatcc
      601 agagtcagct cgacagtatg gtcaacggcg agcaatcgca gattgtgagc ttaagagcgc
      661 aacttactga agcaaaatat aatcttgagc agactgtcat tcgcgcacca agcaatggct
      721 acgtcactca ggtactgatc cgcccaggta catacgcagc tgccttgccg ctgcgtccgg
      781 tgatggtctt catccccgag caaaaacggc aaattgtcgc ccaatttcgg caaaactcgc
      841 tgttacgtct gaaacctggc gatgatgcgg aagtggtgtt taacgcgcta cctgggcagg
      901 tgttccacgg caaattgact agtattttac ctgtcgtgcc aggcggttct tatcaggcgc
      961 agggggtatt gcaatcatta acggtcgttc ccggcacgga cggtgtgctg ggaaccattg
     1021 aactggaccc taacgatgat atcgatgcct tacccgacgg catctacgcc caggtggcgg
     1081 tttactccga ccatttcagc catgtttcgg tgatgcggaa agtgctgcta agaatgacca
     1141 gttggatgca ttatctttat ttggatcatt gagacttata tcacctgtgt tttgcgtgag
     1201 cctgagccat actgatattt ttcctcgcac aaaggactcg gcatgaaact cgtcggtagc
     1261 tacaccagcc cgtttgtacg caaactttct attctgttgt tagaaaaggg cataactttc
     1321 gaatttatta atgaactgcc ctataacgcg gataacggcg tggcgcaatt taacccgtta
     1381 gggaaagtgc cggtgctggt gaccgaagag ggcgaatgct ggtttgattc gccgatcatc
     1441 gctgaatata ttgagttaat gaatgtcgct ccggcgatgt tgccgcgcga tccgctggag
     1501 tcgttgcggg tgcgcaaaat tgaggcgctg gcggatggca ttatggatgc cgggctggta
     1561 tcggtgcgtg aacaggcgcg tccggcggcg cagcagtctg aagatgaatt gttacgccag
     1621 cgggagaaaa tcaaccgcag tctggatgtg ctggaaggat atctggtcga tggcacactc
     1681 aaaaccgata cggtcaatct ggcgactatc gccattgcct gtgctgtcgg atatctcaat
     1741 ttccgccgcg ttgcgccagg ttggtgtgtc gaccgcccac acttagtcaa actggtcgaa
     1801 aacctgttta gccgcgaaag ttttgctcgc accgaaccgc caaaggcttg atgcgcgata
     1861 tgtcctcctg acccatctca cgttacaatc cgtggttatg ttaaacgccc ttctccgtgt
     1921 gagagggcct tgatcagcca ggtttcctat gacaaccgaa acgcgttccc tctatagtca
     1981 acttccggct attgatcgct tattgcgcga tagctccttc ctttctttgc gtgatactta
     2041 tggtcacacc cgcgtggtgg aattgttgcg tcagatgctc gacgaagcgc gagaagtgat
     2101 tcgtggcagc cagacgctgc ctgcgtggtg tgaaaactgg gcgcaagaag tcgatgcccg
     2161 gttgacgaaa gaggcgcaga gcgcgctgcg tccggtgatc aacctgacgg gaaccgtgct
     2221 gcataccaac cttgggcgag ctttacaggc ggaagccgcg gtggaagccg ttgcgcaggc
     2281 tatgcgttcg ccagtgaccc tcgagtacga tctggacgac gccggacgcg gacatcgcga
     2341 tcgggcgctg gcgcagctgc tgtgccgtat tacgggggcg gaagatgcct gtatcgtcaa
     2401 taacaatgcg gcggcggtgt tattgatgtt ggcggccact gccagcggga aagaggtggt
     2461 ggtatctcgc ggcgaactgg tggagattgg cggcgcgttt cgtattcccg atgttatgcg
     2521 tcaggcaggc tgcaccctac acgaagtagg gaccaccaac cgcacgcacg cgaatgatta
     2581 tcgtcaggcg gtgaatgaaa ataccgcact gttgatgaaa gtacatacca gtaactacag
     2641 cattcagggg ttcaccaaag cgatagacga agctgaactg gtggcgctcg gcaaagagct
     2701 ggatgttcca gtggtgactg atttaggcag tggctcgctg gtcgatctta gccagtacgg
     2761 tttgccgaaa gagccaatgc cgcaggagtt gattgcggcg ggcgtcagtc tggtgagctt
     2821 ctccggcgac aagttgttag gcgggccgca ggcagggatt atcgttggta aaaaagagat
     2881 gatcgcccgc ctgcaaagcc acccgctgaa gcgtgcatta cgcgcggata aaatgaccct
     2941 cgcggcgctg gaagccacgt tgcgtcttta tttacaccct gaagctctga gtgaaaaatt
     3001 accgaccctg cgcctgctta cccgcagcgc agaggtcatt caaatccagg cacaacgttt
     3061 acaggctccc cttgtcacac attacggcgc ggagtttgcg gtacaggtta tgccatgtct
     3121 ttcgcagatt ggcagtggtt cgctgccggt tgatcgcctg ccgagcgcgg cattaacgtt
     3181 tacaccccat gatggacgcg gtagccacct tgagtcatta gccgcccgct ggcgtgaatt
     3241 gccagtgccg gtgattggtc gtatttatga cggacgattg tggctggatt tacgctgcct
     3301 tgaagatgag caacggtttt tggagatgtt gttgaaatga ttattgcgac tgccggacac
     3361 gttgaccacg gcaaaacaac cttattgcag gcgattactg gcgtaaatgc tgaccgtctg
     3421 ccggaagaaa aaaagcgcgg catgaccatc gatctcggct atgcctactg gccgcagccg
     3481 gatggtcgcg tgcctggttt tatcgacgtt cccggtcatg aaaagtttct ttccaacatg
     3541 ctggcgggcg ttggtggtat cgatcacgcg ctgttggtgg tggcgtgcga tgacggcgtg
     3601 atggcacaga cccgtgagca tctggcgatt ttgcagctga ccggtaaccc gatgctgaca
     3661 gtggcgctga ccaaagccga tcgtgtggac gaagcgcgtg ttgatgaggt tgaacgccag
     3721 gtaaaggagg ttctgcggga atacggtttt gctgaggcaa aactgtttat caccgcagca
     3781 accgaaggtc ggggaattga tgccctgcgc gagcatctgc ttcagttgcc ggaacgcgag
     3841 cacgccagcc aacatagttt ccgcctcgcg attgaccgcg catttaccgt aaaaggtgcc
     3901 gggctggtcg tcaccggtac ggcgttaagc ggggaagtga aggtaggcga ttcactctgg
     3961 ctgactggtg taaataaacc gatgcgtgta cgtgcgctgc atgcgcaaaa ccagccaaca
     4021 gaaaccgcca atgccgggca gcgtatcgcg cttgacatcg cgggtgatgc ggaaaaagag
     4081 cagattaacc gtggcgactg gctgcttgcc gatgtgccgc cagagccgtt cacacgggtg
     4141 attgtcgagc ttcaaaccca tgcaccgctg acccagtggc agccgctgca tattcaccac
     4201 gccgccagcc acgtcacggg acgcgtttca ctgctggaag ataaccttgc tgaactggtc
     4261 ttcgacaccc cgttatggct ggcagataac gaccgcctgg tattgcgcga tatctctgcc
     4321 cgcaacacgc tggccggagc gcgcgtcgtg atgcttaacc cgccgcgtcg cggtaaacgt
     4381 aaaccagagt atctgcaatg gctggcttcc ctcgctaggg cacagaatga tagcgaagcg
     4441 ttagccgttc acctgcaacg cggcgcggtt aatcttcctg atttcgcctg ggcgcgccag
     4501 ctcaacggcg aagggatgcg cgaactgctg caacagcctg gctatattca ggctggttat
     4561 agcttgttga atgcgccggt tgccgctcgc tggcaacgga aaattctcga cacattagcg
     4621 acttatcatg agcaacatcg cgatgaacct gggcctgggc gcgaacgtct gcgacgtatg
     4681 gcgttgccaa tggaagatga agcgctagta ctgttgctga ttgaaaagat gcgcgaaagc
     4741 ggcgacatcc tcagtcatca cggttggctg catctgccgg atcacaaagc gggcttcagc
     4801 gaagagcagc aggccatctg gcaaaaagca gagccgctgt ttggtgacga gccgtggtgg
     4861 gtgcgtgatc tggcaaaaga gacgggtacc gacgagcagg caatgcgcct gacgctacgc
     4921 caggcggcgc agcaaggaat aattaccgcg atcgtcaaag atcgttatta ccgtaacgat
     4981 cggattgtcg aatttgccaa tatgatccgc gatctcgatc aggagtgtgg ttcaacctgc
     5041 gcggcggatt tccgcgatcg cttaggcgta ggccgaaagc tggcaattca gattctggaa
     5101 tattttgacc gcattggctt tacgcgtcgt cgtggaaatg atcatttatt gcgcgacgca
     5161 ttattatttc cggaaagata aggaaatgat taattaattt ttaaaataaa ttaatacaaa
     5221 tttcttatga ttttaaataa agcacattgt ttaatgaata caatgtgctt tttattagat
     5281 taattttgat aattaataat aatgatcatg ttttcgaatg aaaatcctca gtaagctgcc
     5341 cacccttttt tacactttca ggagtgtgtt atggcatctt caactttctt tattccttct
     5401 gtgaatgtca tcggcgctga ttcattgact gatgcaatga atatgatggc agattatgga
     5461 tttacccgta ccttaattgt cactgacaat atgttaacga aattaggtat ggcgggtgat
     5521 gtgcaaaaag cactggaaga acgcaatatt tttagcgtta tttatgatgg cacccaacct
     5581 aaccccacca cggaaaacgt cgccgcaggt ttgaaattac ttaaagaaaa taattgcgat
     5641 agcgtgatct ccttaggcgg tggttctcca cacgactgcg caaaaggtat tgcgctggtg
     5701 gcagccaatg gcggcgatat tcgcgattac gaaggcgttg accgctctgc aaaaccgcag
     5761 ctgccgatga tcgccatcaa taccacggcg ggtacggcct ctgaaatgac ccgtttctgc
     5821 atcatcactg acgaagcgcg tcataacaaa atggcgattg ttgataagca cgtgactccg
     5881 ctgctttctg tcaatgactc ctctctgatg attggtatgc cgaagtcact gaccgccgca
     5941 acgggtatgg atgccttaac gcacgctatc gaagcgtatg tttctattgc cgccacgccg
     6001 atcactgacg cttgtgcact gaaagccgtg accatgattg ccgaaaacct gccgttagcc
     6061 gttgaagatg gcagtaatgc gaaagcgcgt gaagcaatgg cttatgccca gttccttgcc
     6121 ggtatggcgt tcaataatgc ttctctgggt tatgttcatg cgatggcgca ccagctgggc
     6181 ggtttctaca acctgccaca cggtgtatgt aacgccgttc tgctgccgca tgttcaggta
     6241 ttcaacagca aagtcgccgc tgcacgtctg cgtgactgtg ccgctgcaat gggcgtgaac
     6301 gtgacaggta aaaacgacgc ggaaggtgct gaagcctgca ttaacgccat ccgtgaactg
     6361 gcgaagaaag tggatatccc ggcaggccta cgcgacctga acgtgaaaga agaagatttc
     6421 gcggttctgg cgactaatgc cctgaaagat gcctgtggct ttactaaccc gatccaggca
     6481 actcacgaag aaattgtggt gatttatcgc gccgcaatgt aatcatcatt ccaacaacgg
     6541 ctggcaaatt gtcagccgct ttttcaacta tctctgtaat ccttgcccgt aaattcgtga
     6601 tagctgtcgt aaagctgtta ccgactggcg aagatttcgc cagtcacgtc tacccttgtt
     6661 atacctcaca ccgcaaggag acgatcatga ccaataatcc cccttcagca cagattaagc
     6721 ccggcgagta tggtttcccc atcaagttaa aagcccgcta cgacaacttt attggcggtg
     6781 aatgggtagc ccctgccgat ggtgagtatt accagaacct gacgccggtg accgggcagc
     6841 tgctgtgcga agtggcgtct tcgggcaaac gagacatcga tctggcgctg gatgctgcgc
     6901 acaaagtgaa agataaatgg gcgcacacct cggtgcagga tcgcgcggcg attctgttta
     6961 agattgccga tcgaatggaa caaaacctcg agctgttagc gacagctgaa acctgggata
     7021 acggcaaacc cattcgcgaa accagtgctg cggatgtacc gctggcgatt gaccatttcc
     7081 gctatttcgc ctcgtgtatt cgggcgcagg aaggtgggat cagtgaagtt gatagcgaaa
     7141 ccgtggccta tcatttccat gaaccgttag gcgtggtggg gcagattatc ccgtggaact
     7201 tcccgctgct gatggcgagc tggaaaatgg ctcccgcgct ggcggcgggc aactgtgtgg
     7261 tgctgaaacc cgcacgtctt accccgcttt ctgtactgct gctaatggaa attgtcggtg
     7321 atttactgcc gccgggcgtg gtgaacgtgg tcaatggcgc aggtggggaa attggcgaat
     7381 atctggcgac ctcgaaacgc atcgccaaag tggcgtttac cggctcaacg gaagtgggcc
     7441 aacaaattat gcaatacgca acgcaaaaca ttattccggt gacgctggag ttgggcggta
     7501 agtcgccaaa tatcttcttt gctgatgtga tggatgaaga agatgccttt ttcgataaag
     7561 cgctggaagg ctttgcactg tttgccttta accagggcga agtttgcacc tgtccgagtc
     7621 gtgccttagt gcaggagtca atctacgaac gctttatgga acgcgccatc cgccgtgtcg
     7681 aaagcattcg cagcggtaac ccgctcgaca gcgtgacgca aatgggcgcg caggtttctc
     7741 acgggcaact ggaaaccatc ctcaactaca ttgatatcgg taaaaaagag ggcgctgacg
     7801 tgctcacagg cgggcggcgc aagctgctgg aaggtgaact gaaagacggc tactacctcg
     7861 aaccgacgat tctgtttggt cagaacaata tgcgggtgtt ctaggaggag atttttggcc
     7921 cggtgctggc ggtgaccacc ttcaaaacga tggaagaagc gctggagctg gcgaacgata
     7981 cgcaatatgg cttgggcgcg ggcgtctgga gccgcaacgg taatctggcc tataagatgg
     8041 ggcgcggcat acaggctggg cgcgtgtgga ccaactgtta tcacgcttac ccggcacatg
     8101 ctgcgtttgg tggctacaaa caatcaggta tcggtcgcga aacccacaag atgatgctgg
     8161 agcattacca gcaaaccaag tgcctgctgg tgagctactc ggataaaccg ttggggccgt
     8221 tctgatataa gaagctggtc gcattgggta ttcattgcct gatgcgacgc ttacgcgtct
     8281 tatcatacct acgggaacct gaccgtaggc cggataaggc gtacacgccg catccggcat
     8341 aaacaaagcg cacgttgtta caatttacgt tttaaaccta cgtgcgcaat aacccagact
     8401 acgccaacag aagtgcgttt ctggggcgtg ttttgtcagg agtttaatct cgcgtttcga
     8461 tggtgagcgg gcgaaccagg ccgtaaatca ccattggtga tccgaggcca atcagcaata
     8521 aacggtagat aattgaaggt ttaatacgct taatctgtac tgtggatgtt ttactggagt
     8581 tattcattag tttggccaat agttccacta tttcaatatg ataaacctcg tcctgttgaa
     8641 aaaccagaca gcacatttca gaaagtatat aaaaaacaat atgtcgttat ataaataagg
     8701 aaattgcact gcatcatggc atttcataat atgcgttaat tgttcttttt tatattcttt
     8761 gagggaaata tgttcctgga ctattttgca ctgggagtgc ttatttttgt atttctggtg
     8821 atattctatg ggattattat tttacatgat attccctacc tgatttctaa aaaacgtaat
     8881 catcctcatg ccgacgccat tcatgttgct ggttgggtga gtctttttac gctgcatgtt
     8941 atctggccat ttttgtggat ttgggccacg ctttaccgcc cggagcgggg atggggaatg
     9001 caaatccatg actcctccgt tgtgcaacta caacagcgta ttgccgggct ggagaaacag
     9061 ctcgccgaca tcaaatcctc ttctgccgag taatacttat ggatttactg attattttga
     9121 cctatgtggc ttttgcatgg gcaatgttta agatcttcaa aattcctgta aataaatgga
     9181 ccatttccac agcggccctg ggtggcatat ttattgtcag tggtttaatt ctgttaatga
     9241 actataacca tccgtatacc ttcaaggcgc aaaaagcggt tatttctatt cctgttgtcc
     9301 cacaggtgac aggcgtggtg atcgaagtga cggataagaa aaatacgctg attaaaaaag
     9361 gtgaggtgct atttcgactg gacccgacgc gttatcaggc gcgggtggat cggctgatgg
     9421 cggatatcgt taccgcagaa cataaacagc gggcgttggg cgcagagtta gatgagatgg
     9481 cggcgaatac tcagcaggca aaggccacgc gggataaatt cgctaaagag tatcagcgtt
     9541 acgcacgcgg cagccaggcg aaagtaaacc cgttttcaga acgcgatatt gatgtggcgc
     9601 ggcaaaatta tctggcgcag gaagcctccg taaagtcatc ggcggcggaa caaaaacaga
     9661 tccagagcca gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc agcctgaaag
     9721 cgcagctcgc ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg ccgagcgatg
     9781 gttatgtgac ccaggtgctg attcgtccgg gtacctatgc cgcgtcgctg ccgctacgtc
     9841 cggtgatggt gtttataccc gatcagaaac gacaaattgt ggcgcagttc cgtcagaact
     9901 ccttgctgcg actggcccct ggcgacgatg cggaagtggt gtttaatgct ctgccaggta
     9961 aggtattcag cggcaagctg gcagccatta gtccagctgt tcccggcgga gcttatcagt
    10021 cgaccggcac cttacagacg ttaaacacag cgccgggttc agatggcgtt atcgcgacca
    10081 ttgaactgga tgagcacact gatttgagcg ctttaccaga cggtatttac gcccaggtgg
    10141 cggtctactc tgatcatttc agccatgtct cggtgatgcg caaagtgcta ttacgcatga
    10201 ccagctgggt gcattacctt tatctcgatc attaatcttc gctttccgcc gcttgttcag
    10261 gccgcagttg ctgcgactga gcaagctggt ggcgaatttc acgcagcatt tcatccagca
    10321 catactggac ccgccagggc aggtattccc gtttacggaa gatagccacc agatccagcc
    10381 accattcata cgggtgctga aagcagggca ccagcgtgcc gtcttgcaga tcgctctgca
    10441 cacttttgtc tggcgcaaac acaatcccca ggtgatttcg tgccagctcc agcgccgatt
    10501 gcgtgttatc gcaaacgtaa tttcccttca cgcgataatc ccgcacctct ttactcccgg
    10561 cgacgttgaa gcgccagata ttcgcgtcgt cgatcatcat cgagtcgatc aaaatacagg
    10621 agtgatgttc gagttcgtca ggacggctaa taggatgttt ctcaagatag cgctggctgg
    10681 cataagcggt tacggcatat tgtgtaataa aactggcaac cagcgattca tcttttggtg
    10741 gagcgtagct gattaaaaca tcacaatcat caggaaattc gacgccttca taaaaggcat
    10801 tacgctcaa
//
LOCUS       DADLTF010000134        10461 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.134, whole genome shotgun sequence.
ACCESSION   DADLTF010000134 DADLTF010000000
VERSION     DADLTF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.134"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="JN749_003288"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="JN749_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HAY7369602.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAEMVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="JN749_003289"
     CDS             complement(1151..1237)
                     /locus_tag="JN749_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HAY7369603.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JN749_003290"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="JN749_003290"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HAY7369604.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JN749_003291"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="JN749_003291"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HAY7369605.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JN749_003292"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="JN749_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HAY7369606.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     EMEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGCDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="JN749_003293"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="JN749_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HAY7369607.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="JN749_003294"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="JN749_003294"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HAY7369608.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="JN749_003295"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="JN749_003295"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HAY7369609.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="JN749_003296"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="JN749_003296"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HAY7369610.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="JN749_003297"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="JN749_003297"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HAY7369611.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..>10461)
                     /locus_tag="JN749_003298"
     CDS             complement(10298..>10461)
                     /locus_tag="JN749_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7369612.1"
                     /translation="ATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVAL
                     FLTAKVKDV"
BASE COUNT         2736 a         2743 c         2561 g         2421 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggcttagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccatc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccagcc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aagcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaatgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 acccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccatttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgtttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattatt ttatatattt aatgcattaa
     5041 ttcttaacat taattgatca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca ttaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tggacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccagg
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcgc caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaagctcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc ctgtgcgtgc gtgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccggtctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttccg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact cgcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgcgatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct g
//
LOCUS       DADLTF010000135        10413 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.136, whole genome shotgun sequence.
ACCESSION   DADLTF010000135 DADLTF010000000
VERSION     DADLTF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.136"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..182
                     /locus_tag="JN749_003299"
     CDS             <1..182
                     /locus_tag="JN749_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7369613.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            525..1241
                     /locus_tag="JN749_003300"
     CDS             525..1241
                     /locus_tag="JN749_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7369614.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLDAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1697..1772)
                     /locus_tag="JN749_003301"
     tRNA            complement(1697..1772)
                     /locus_tag="JN749_003301"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1737..1739),aa:Asn,seq:gtt)
     gene            complement(1873..3500)
                     /gene="yeeO"
                     /locus_tag="JN749_003302"
                     /pseudo
     CDS             complement(1873..3500)
                     /gene="yeeO"
                     /locus_tag="JN749_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            3505..3580
                     /locus_tag="JN749_003303"
     tRNA            3505..3580
                     /locus_tag="JN749_003303"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3538..3540,aa:Asn,seq:gtt)
     gene            complement(3618..4568)
                     /gene="cbl"
                     /locus_tag="JN749_003304"
     CDS             complement(3618..4568)
                     /gene="cbl"
                     /locus_tag="JN749_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HAY7369615.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4670..5587)
                     /gene="nac"
                     /locus_tag="JN749_003305"
     CDS             complement(4670..5587)
                     /gene="nac"
                     /locus_tag="JN749_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HAY7369616.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5914..5989
                     /locus_tag="JN749_003306"
     tRNA            5914..5989
                     /locus_tag="JN749_003306"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5947..5949,aa:Asn,seq:gtt)
     gene            complement(6045..6980)
                     /gene="ldtA"
                     /locus_tag="JN749_003307"
     CDS             complement(6045..6980)
                     /gene="ldtA"
                     /locus_tag="JN749_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HAY7369617.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7042..8121)
                     /gene="cobT"
                     /locus_tag="JN749_003308"
     CDS             complement(7042..8121)
                     /gene="cobT"
                     /locus_tag="JN749_003308"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HAY7369618.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(8133..8865)
                     /gene="cobS"
                     /locus_tag="JN749_003309"
                     /pseudo
     CDS             complement(8133..8865)
                     /gene="cobS"
                     /locus_tag="JN749_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
     gene            complement(8862..9407)
                     /gene="cobU"
                     /locus_tag="JN749_003310"
     CDS             complement(8862..9407)
                     /gene="cobU"
                     /locus_tag="JN749_003310"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HAY7369619.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(10034..10297)
                     /locus_tag="JN749_003311"
                     /pseudo
     CDS             complement(10034..10297)
                     /locus_tag="JN749_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2961 a         2631 c         2212 g         2609 t
ORIGIN      
        1 gtgtttcaga taaaccgtta catggtgaga ttaagcttcc cggccaggct aaccgttttt
       61 atgaaggcgc tatttccgaa cacctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg
      121 cagaaggcga ccgactgcat tcacgtaaat tacgaacctt taatgagccg ccgttccgat
      181 aataagttat gaaccttctt atcagaaata gatgtgaagg attaagtaag acatctggca
      241 aatagcctgc aataaacgcg ggctgcttgc ttccttgggc agatgcgagt tttattatcg
      301 tcttaatgat ttccacatat taaaagcaag tatgctttca aaacacaatt ataaaaaatc
      361 ccgccaacaa cattagtttt tataaaatta aatataagat tatggcttta gaatattttt
      421 atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa cacatgttaa
      481 cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga cgtaaatggg
      541 ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt tatgcaaaat
      601 tcggtgtaga aatctatgct gctgccaaac aaggtgaacc cgatccagaa ttaaacacat
      661 ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac gttattgata
      721 aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtacaggga cgttatgaag
      781 gctttggtcc taatggctca atgattattg ctgagacatt gacttcaaat gttaaccgta
      841 cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga gcggcaggtt
      901 ctgtcagcta tatgtttgac aatacgggtg tgattgtatt taaaggaaca gaccctgacc
      961 atatttttga aattttgctt gacgctgaag ttgatgttcg tgatgtaact gaagaagaag
     1021 gtaatattgt tatttatact gaacctgctg accttcataa aggaatcgcg gcgctaaaag
     1081 cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa tctgaagttg
     1141 agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt gaagatgacg
     1201 acgatgtaca aaaagtttat cataacgtcg caaatctcta attatatttt aaagaaatct
     1261 gtctttatgg cagatttctt taatctcata taattcttat aaaaaatata atgtttaact
     1321 cgtcatattg cttataaaaa atccccgttc ccagagaaat aatatttatt aaaatggcag
     1381 gtcttctttt tctgattacc gaagacaaag tggcaattac gtatagtttc ccgataaaga
     1441 cgcgacagct acatcccgca taaggcattt ttctctttat ctttgtacgg tacttcatgg
     1501 aacagaattt ttgaccttgt gaatcgtgat gtctgttggg gaggggcaat ttgctcactg
     1561 aagcgtgaga ctcgattaag cgcacgaaac acagaaatca aaaaacccgg ccactttttt
     1621 acaaagtggc cgggtaaaaa taatttttat tttttaactg tcctgagtct catagagatg
     1681 tctcaaaact aaaatttggc tcctctgact ggactcgaac cagtgacata cggattaaca
     1741 gtccgccgtt ctaccgactg aactacagag gaatcgtgag aacgaggcga atattagcga
     1801 tgcccaccca caatgtcaaa gcctgttttt taaatttgaa atcgtttgct gaaataatct
     1861 gcattttgtc gtttattccg acacaactgg ctttttttca cacttttgcg gctcggatcg
     1921 agggtatttc cacagccaac gtccagtaac cattcgccag taaaacagca cggcccgcac
     1981 agcccagtag gcaaacattc ccatccagac accaaccaca ccccagccaa gcatgattcc
     2041 ctgcacataa ccgactacaa cccggcaacc ccacatgctc aacatagaaa cccacatggc
     2101 gtaacgggca tcacgagcac ccttaaatcc agcgggtagc acccatgagg cggaccaaat
     2161 aggcataaat aaagcattta gctaaatcag aatcacaacg acgtgtttaa cctgtggatc
     2221 ctgggtgtaa aacgatgcca taaccccggc aaagggagcc gttagccagg cgatggccgt
     2281 taatccaaga gtggacagcc agaacacatg ccgcaactga atctctgctt gcgctatctg
     2341 ccctaccccc aaccttcggc ctgtaatgat cgtagaagca gaaccgagcg cacttccggg
     2401 taagttgata agagccgcaa ttgagaacgc gataaaattt ccggcaataa cgctggtccc
     2461 catcccggca acgaacattt gggttaataa ccgaccactg gtaaataaca ctgattcgac
     2521 actcgctgga ataccaatcc ccatgacttc ccagataatg ctaaaattca gcggtttaaa
     2581 atagctcttt aacgaaatcc ttagcgcagg attaaaacca atcgccagca cccacaaaat
     2641 tgcaactgcg ccaatataac gagaaatggt taaacccaac ccagccccga ccaggagaaa
     2701 aggccgtaaa tcaatatgcc gctaataata atattaagaa tattcaggct accgttaatc
     2761 agtagcggta ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat aagagtgatg
     2821 gcagcggctg gataactgag tacggtcagc tccagataag tcaacgccag tgctttaact
     2881 tctgtcgtgg catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaattagc
     2941 gttgccaaca gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc
     3001 gctcgtcgtc gatcccgctt accgagacta aatgctacaa cgacagtagt accaagatcg
     3061 atagctgcaa aaaaagccat aatgaccata ttgaagctgt ccgccaaccc cacgccggcc
     3121 atcgcatctt ttcccagcca gctgaccaga aaagtgctca ggccccccat caacaggaca
     3181 cagacgttct ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact
     3241 ttgtagctct tgcgtttaga gtgccagcga gtgccgtgaa caacctggcg taaagcagag
     3301 gagatattca aagccgacct taattgcaga aagtgaaacc acatttcaaa tgatgaggga
     3361 gaatcagcaa agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt
     3421 gatagaaacg gcaaacagtt gggaaattta aaaatcgggt ttgacaaaat atttttcgcc
     3481 gttaagatgt gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt
     3541 aatccgtatg tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt
     3601 ttatagcggg ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc
     3661 tggcgcttga tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccaaaca
     3721 tagttacgct gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga
     3781 tgccgcgtat ccaggcggat taaattctct tcctcttgtt cgccgctgga ttgttcggca
     3841 actaatccga tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgtgca
     3901 cttaatacaa tatctgccgg caaacctttg cgggtaaatg cgtcatcagt gcgtgagcgc
     3961 cccgtaattc cctgtcggta agtaattaac ggccacttcg ctattgattc cagcgtcaat
     4021 ggtgtaattt gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccaacaaaac
     4081 cacgggaagg cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca
     4141 gcttcgccat tttgcaacaa cgtcgcaatt tcctgtggcg aaccctggat tagctcgagc
     4201 cgaacctccg ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc
     4261 tgagtatgcg tcgttgcaat agtgagaaca ccagacgtat cgttggtaaa caggtctgca
     4321 agccgacgaa cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg
     4381 cccggttcag tcatccccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt
     4441 tcatcctcca gttcccgaat atgacggcta acgcctgact gtgaggtaaa aagcatattc
     4501 gcaacctctg tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg
     4561 aaattcacgg taaactccgg gcagttcaga tatcccgtta ttgttaaagt ctaatgcccg
     4621 gcataacaaa taataaaaac ccgcatctta ttccatcccg atataacgct tagctcacca
     4681 attgccactg ccttttttcc atcactggag aactaatcac tgacattaac aactctttca
     4741 ctgcctgtgc ctgtggtgac aagtttgctc tggcgggtaa atttaaggac aaagagagac
     4801 tcattgaagg agtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg
     4861 cagccgattc aggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg
     4921 caatagactc aatttcacca atgacttttg ccgtgagccg ccgtagggaa aaagcctcat
     4981 caacacgaag tctaacagcg ctgtaatcac tggggagaaa aaggttcatt tgcgcaatag
     5041 cattaacatc aacgttttgc cccgggcaat cttgagttcc taccaggaaa agatcttctt
     5101 tcagcaaagc ctgactggat accccagcca caggggaatg ctcataaatc accgccatat
     5161 cgagttggtg atttatcagt ttttcgttaa gcactgcgcc actattttca tgaagataga
     5221 taacgacctc cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg
     5281 cagcggttcc tggcgcaaag ccaatcgaga cttgccccga taatgactga ccaacgttat
     5341 gcaccgccag ttgggcctgc tcacactgac gtaaaatggc ccgtgcatgg gtatagagaa
     5401 tttttccggc gtctgttggc gtaacgcccc gttttgtgcg aatcaaaagc tgttgattta
     5461 actcaccttc cagtatggca acctgctggc tgagcgctgg ttgtgcgata tgcaatactt
     5521 cagcagcctg ggtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga
     5581 agttcatgtt gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag
     5641 tatgcaagat gcctgccagt tctattttgg cgttgatgcg caagctaacc aactgtcaaa
     5701 taagagatta tgatagattc gtcattggcc ctttttatca gcagtcgcat tcacctgccc
     5761 tataaaagga gggtatgcac cacgatggtt cattacccaa taagattgaa agctcaccac
     5821 tttgttgaaa ttggcagcaa acaaacaaaa aaatgcattt caccctttga catcaccatg
     5881 cactgccatt aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg
     5941 cggactgtta atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa
     6001 gcctgctttc gagcaggctt tttactttct aattaccaac gctcttaaaa catctgtctt
     6061 gaaccagaac taatttgcac aggcattccc gatcgatgtt gcaacgcagc atttgcgcga
     6121 tttacatcaa cttcttgccc gttgataaac gcccgcaaag atggggttac cggcaatggc
     6181 acttttcggt cagactcata ttctgcacga ttgcgtgaca gcggctcatg aacttccagc
     6241 cactttgaac catccggttc cgttgtgtat ttcactggct gatcaataat ttgtacacgt
     6301 gtgcctacag gaacattatc aaacagatat ttgatatcgt cattacgcag acgaatacag
     6361 ccctgactta cacggagccc aataccaaaa ttggcattgg taccgtggat ggcatacaac
     6421 ctgccaatat aaatcgcgta cagccccatg gggttatcgg gccccgcagg aacaaatgcg
     6481 ggcaaactct cccctcgttt cgcatattcg cgccgggtgt tcggcgttgg cgtccaggtt
     6541 ggtgcttctt gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc
     6601 tggccgatac caataggaaa gacttccact gtattactgt ctggcggata gtaataaaga
     6661 cgcatttcag cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc
     6721 tgcggaatgg tgagttgtga gccaggcttc ggtaaaaaaa catcagcgcc cggattcgct
     6781 tccagcatat tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgagta
     6841 ttgtgatcag gaacagttac agtaagcgac tgccccacta aacggctacc ctctggaggt
     6901 aatggataag ttaccgccag gctattttgg ctggcaaaaa gcagagcaaa tgagcaaaga
     6961 atatttacac gacgcatcat gtccctttcc taagtcgcga atactatccg ataagtatag
     7021 cttttatcag acttttcgtt tttagctgtt caaatcagaa gtcgtattcc ccggtagaac
     7081 aatattactg gcagcaagtt cgcccatgtt gttgtatatc gcacaagcag cttcgatgat
     7141 ggacatcgcc agagctgcac cactcccctc acctaaacgc atatccatat tgagataagg
     7201 ctccagcccc aaatgcgaga gcgctatacg cgcgcctttt tctgccgaca agtgagaagg
     7261 aatgagatac ggtttgattg caggagacat ctggcaggct gcgagcgcag cagcataaga
     7321 aagaaatcca tccagcaaca cgggtaaacc gcaggaagca gcacctaaca tcactccagc
     7381 tattccgacc aaatcaaatc cacccacttt tgccaggaca tcaacaccat cctgaggatt
     7441 tggttgattc aacgtaatcg cccgacgcac aacatcaatt ttattagcca gtttatctgt
     7501 cggcaggttt gcgccaatcc caaccacttc ttcaggatcc cggccagtga ttgtgctgac
     7561 tattgccgct gccggtgtcg tgtttgccat ccccagttca cctacaccaa acagcgtgac
     7621 accgtttttc gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttcagcctg
     7681 acggcgactc attgccggag ctgaagcaat attgccgcta cctcgtgcga cacgcatgtt
     7741 gataagcccg gggataggct cagcagtatc aataccaaca tcaattacgt ggacgttagc
     7801 gcccgcttgt gctgccagca cacacacgcc ggttgttcca cgggtcatat tttcagcctg
     7861 tatggctgtc acttcttttg gagaaatagc gaccccttcc tcccagacgc cgtgatcggc
     7921 acacataacc agtaccgctt ttttgcccac atgcggtatg ccattcaccc ccggcattcc
     7981 tgccagttgt atggcaagcg cctccagctt tcctaggcta ccaacaggtt tgagtaaccc
     8041 gtcaacatgc cgttgtgcac gcgacatagc ggcagaattg atggctggga tcgtattcag
     8101 taaatcggca attatttgca tgtcatgtct ctttatagca gtgccagcag gaagaccagt
     8161 tcaccaagct ctatggctgc tcccagcgta tcgcccgttt gcccgcccag cgtacgtttg
     8221 agaagctgac cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc
     8281 atacccagca ataatacggc agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt
     8341 cgcgccaatg aacacattgc caagcccttc ttcccgcgca taacgatgac gatacatcaa
     8401 taatgcggca gtcccgcggc tgaccgcaca tgctgccgcc agcgatgcaa ggatcggttc
     8461 gccacgcaga gccaactcgc ttaacaccag aatctttgcc agtaccacaa aaatcaatgc
     8521 cagaccgccg tgggtgccta aacggctatc acgcatgatt tccaacatgc gatcgcggct
     8581 acgtgcagaa aatacgccat cacaggtatc agcgaggccg tccagatgga attccccggt
     8641 catcagcgcc agcacaagta cgctaaacag tgccgccagt ggtgcgccac accatgcctg
     8701 cagcaccatg aagaccagcc cgctaatcgc gccaagcaat aatccaatca aaggaaaagt
     8761 aataataccg cgagaataat gctcgaaatc cagtccctgg gaccagcgac gcggtacagg
     8821 cagacgcgta ataaatgaga gcatcgccca aaataatttg ctcatttgat ttttactcca
     8881 atacccgaaa ccaccagcca tacttcattt gccgcagcgg ccaactgctg atttacccgc
     8941 ccggcaatat cacgaaaatg tcgtgccaga cgactctccg gcacaatccc cattcccact
     9001 tcgttagtca ctaatacaac ctttgcgggg caacgttggc aggcagcaat caacgactga
     9061 atctcagcat taatcgcctg ttccatcgcc tgataatccc attcatcagg gtctttatcg
     9121 ccgccataat caaacaacag attagtcacc attgtggtaa cgcattccag caacacagcc
     9181 tcattcgggt taatgtctgc atgaattaat tcatcaagat gttgccagcg ctccactgtg
     9241 cgccagtgct ccgggcggct ttgccgatga tgttctatcc gtgcagccat ctcatcatca
     9301 aggatttgcg aggtagcgat atacagaacc tgtgaagagt ccccaataag cacctctgca
     9361 tggcgactct tcccgctcca tgcgcctccc gtcaccaaaa tcatcatacc gactcctgat
     9421 tcaaatgtat gatggtatac attgaacaga gcaatggatc ccccaccgtt gctgaccgca
     9481 aaacatttac gcgattaata ctgcgcgtaa tataattaaa aatccagcat tctcaatcca
     9541 ttacgaaata agatggcatt acggaatagc cagactttct cgtgttaacg tccgacaaaa
     9601 tgcgtcattg acagcggttt ctgtggattg acacatttca ttcagataat gaattaatgc
     9661 tactgccgga ccaaccagca aacgggcatt gtgttctgaa atccaggcta ttgattcaac
     9721 gtcagcgaca gatctgcgct gtaaaagaac ttgtaaaaca acgcaataga attatccgga
     9781 atggtggcga ctatgcacta gaacaccaca atttcgctct ctcggtaagg cactgtcaca
     9841 gcattcaaca gaatgtgact tgccagattt attagcgcca ccagatgttt aaccgggtaa
     9901 ccacgaccca gtcggggata tattccatct tctcgctgac atcatcaaga ataaaaaggt
     9961 ttatcacact cagagcaacg ttcttcagca ggcgggataa ttcttcaaca tttaaaagat
    10021 gtgttattaa aggcatctca aattggacag ggctgccggt tttctgttgc aacaccgttg
    10081 ctctgcttga gacagaactg ccatatgcag catctgatac tttgagtttg accaaatcac
    10141 agccccgtag cttactgtcc aaagccatgt tgaacagagc tagatcgcgt gttttacctt
    10201 ccaactcaag tctaatccgg atcccccaga tatgagatat ctgaagtggc cttttttgac
    10261 cgatggtacg gtctttattc cacggtgacg ttttcatatt caaatctcct gcaatgtggg
    10321 agatttgagt atggttgtct ctcaagagcg aaaataagtc aactatcact agttgttgcg
    10381 catgtacgta ttctgtcaat taattattga caa
//
LOCUS       DADLTF010000136        10379 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.137, whole genome shotgun sequence.
ACCESSION   DADLTF010000136 DADLTF010000000
VERSION     DADLTF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.137"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            14..1280
                     /locus_tag="JN749_003312"
                     /pseudo
     CDS             14..1280
                     /locus_tag="JN749_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            1332..2487
                     /locus_tag="JN749_003313"
                     /pseudo
     CDS             1332..2487
                     /locus_tag="JN749_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(2453..2575)
                     /locus_tag="JN749_003314"
     CDS             complement(2453..2575)
                     /locus_tag="JN749_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369620.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2778..3839
                     /locus_tag="JN749_003315"
     CDS             2778..3839
                     /locus_tag="JN749_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HAY7369621.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLINKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTE"
     gene            3933..4463
                     /locus_tag="JN749_003316"
                     /pseudo
     CDS             3933..4463
                     /locus_tag="JN749_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362294.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            4501..6873
                     /locus_tag="JN749_003317"
     CDS             4501..6873
                     /locus_tag="JN749_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HAY7369622.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNTPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6918..9713
                     /locus_tag="JN749_003318"
     CDS             6918..9713
                     /locus_tag="JN749_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAY7369623.1"
                     /translation="MEIIMRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGL
                     RYMIYPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETF
                     ESMGLRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAE
                     RGVISEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQR
                     WYQPNNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDK
                     ENRVNGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTA
                     RSVKIAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWL
                     KNAVDQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQL
                     RKNQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQ
                     AEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIA
                     LANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLIN
                     QRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIA
                     QFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPL
                     TRATDNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIRE
                     QASGAYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQEL
                     NEYQQNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVK
                     QYLSHPVNTYTGVLLPK"
     gene            10075..>10379
                     /locus_tag="JN749_003319"
     CDS             10075..>10379
                     /locus_tag="JN749_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7369624.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
BASE COUNT         3068 a         2290 c         2241 g         2780 t
ORIGIN      
        1 ccgtgccgca ataatgaccg gtcgagctcc cgcccgcttt ggtgtctatt ccaataccga
       61 tgctcaggat ggtattccgc taacagaaac tttcttgcct gaattattcc agaatcatgg
      121 ttattacact gcagcagtag gtaaatggca cttgtcaaaa atcagtaatg tgccggtacc
      181 ggaagataaa caaacgcgtg actatcatga caacttcacc acattttctg cggaagaatg
      241 gcaacctcaa aaccgtggct ttgattactt tatgggattc cacgctgcag gaacggcata
      301 ttacaactcc ccttcactgt tcaaaaatcg tgaacgtgtc cccgcaaaag gttatatcag
      361 cgatcagtta accgatgagg caattggcgt tgttgatcgt gccaaaacac ttgaccagcc
      421 ttttatgctt tacctggctt ataatgctcc gcacctacca aatgataatc ctgcaccgga
      481 tcaatatcag aagcaattta ataccggtag tcaaacagca gataactact acgcttccgt
      541 ttattctgtt gatcagggtg taaaacgcat tctcgaacaa ctgaagaaaa acggacagta
      601 tgacaataca attattctct ttacctccga taatggtgcg gttatcgatg gtcctctgcc
      661 gctgaacggg gcgcaaaaag gctataagag tcagacctat cctggcggta ctcaccaccc
      721 caatgtttat gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg
      781 caatggattt ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta
      841 agctggatgg tgtttccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata
      901 aaaatctgac ctggataacc tcttattctc actggtttga tgaggaaaat attccattct
      961 gggataatta ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca
     1021 ctgaggactt aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata
     1081 cagtagaaaa caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc
     1141 ttgccgccgc caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg
     1201 acagcagcca gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag
     1261 cactaagcga agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga
     1321 gcgagatagc aatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg
     1381 attactgttt ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca
     1441 gcactctgaa agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta
     1501 cctggcaagg cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact
     1561 atcaacaacg ctatgcaggc caaaaacgta tttttaatgc attacaaacg aatggcattt
     1621 tattgaataa tgaatggtgt gcctttctca aagaacatga ttttctggtt ggtatctcga
     1681 tcgatggccc ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt
     1741 ttgcaaaagt gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt
     1801 taaccgtcat taataacgtt aatgtccatt accctcttga ggtttatcat tttttaacat
     1861 ctatcggcag taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg
     1921 atttcagtgg tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg
     1981 cttatggcaa gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa
     2041 ttttcattcg tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt
     2101 gtattttcca ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg
     2161 aatgcgacca ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca
     2221 aaacgatgaa cagtgtacaa ctgacagcgc aaaaaacgga tttcagcgaa atgtcagcaa
     2281 tgtgcatata aacctatctg caatggcggt tgtcctaagc atcgtattac taaagtaaac
     2341 aatgagactg tttcttattt ttgcgaaggt tataaaatcc ttttttcaac catggtacct
     2401 tatatgaacg ccatggttga gttagctaag aacagagtac cgctttacca cattatggat
     2461 gttgcaaaac aaatggagaa taattaataa cgctctcttt taactccatt cttagcctcc
     2521 ttcttatgac cttaacaagg cacccgacat aaattcctgt cgggtgtttc gtcatcatca
     2581 tcttcccttg cgaaaatcct ggcttacgtg gtatgcgtca gggcattagc aatacccttg
     2641 attatcaata gcataaccat aaccactgaa aattaaaaat ttctatatat ttcatattgt
     2701 taaatgatac ttaaataaat ttcatcagta aatcattgcc atagataatc attggatggc
     2761 acaatcaatt ttcgctaatg ataaccattc ccattacgtt ttgtatgttg atagcaaagt
     2821 atctctgtct gctgaagccc ttttggctgc gtaagaataa taaaacttca gtgttgttaa
     2881 tcattattat cctcgcaatg atccttggcg tggttaaaat tcaggtctgg ttaaacgact
     2941 ggaataatga ttttttcaat gcgctaagcc agaaagaaac cgataagctt tggcagcttg
     3001 ttctttggtt ccctgccctg ctggggatct ttgtattaat atccgtaaac aaaacatggt
     3061 taattaaact actgactatt cgctggcgcg aatggttaac agattactac cttaatcgct
     3121 ggtttgcaga taaaaactac tacttcacgc agatctacgg cgagcataaa aatacagata
     3181 accctgacca acgtatcgct gaagacattc tcttactgat caacaaaacg ctcagtcttt
     3241 catttggctt catccagtca cttagcatgc tgatcacctt taccgttatt ctttggcaaa
     3301 gcgcgggtac gctctcattc actgttggtg gaacagaatg gaatatccag gggtatatgg
     3361 tctataccgt cgtgctcatt gtgatcggtg gaactttatt tacccataag gtgggtaaac
     3421 gtattcgtcc gcttaatgtg gaaaaacaac gtagcgaagc gacatttcga actaatcttg
     3481 tgcagcataa taagcaggca gaactgattg cgctaagcaa tgctgagtct ctacaacgcc
     3541 aggagttgcg cgacaatttt cataccatta aagagaactg gcatcgttta atgaatcgtc
     3601 agcggtggct tgattactgg caaaatattt attcgcgctc gcttagcgtt cttccctact
     3661 ttctgttatt gccgcagttt attagcgggc agatcaatct gggcggactg atgaaatcgc
     3721 gccaggcatt tatgctagta tcgaacaatt taagctggtt tatttataaa tatgacgaac
     3781 ttgctgaact ggctgcggtt atcgatcgct tgtatgagtt ccatcaactc actgaatagc
     3841 gccctacgaa taagcctaaa aattgccaac atgcggtaca agtggctaat gcgagtattc
     3901 gtacgcctga taataagatc atattagaga acttgaactt tcatgtttcg ccaggcaaat
     3961 ggctattact gaaaggctac tctggtgcgg gaaaaaccac actgcttaaa acattatccc
     4021 actgctggcc gtgatttaaa ggtgatattt cttctcctgc tgacagttgg tatgtgtcac
     4081 aaacaccgtt aatcaaaagc ggcttactga aagagattat ttgtaaagca cttcccctgc
     4141 ccgtagacga taaatcgttg agcgaagtac tgcatcaggt tggtctgggg aaattggctg
     4201 cgcgtattca tgaccacgat cgctggggag atattctttc cagcggcgaa aaacaacgta
     4261 tcgccctggc acgattaatt ttacgacgtc cgaaatggat atttcttgac gaaactacct
     4321 ctcatcttga ggaacaagag gctatacgct tactgcgttt agtgcgtgaa aaactaccca
     4381 caagcggcgt cattatggtt acacatcaac ccggtgtctg gaacctggct gatgatattt
     4441 gtgacattag cacggtttta taacaacaat aaaagccctg ctgtacggag atataaataa
     4501 atgaagcgag ttcttattcc tggcgtcatt ttatgtggcg ctgatgtggc gcaggccgtc
     4561 gatgacaaaa acatgtacat gcattttttt gaagagatga cggtctatgc tcctgtccct
     4621 gtacccgtaa acggcaacac gcattacacc agtgaaagca tcgagcgttt accgaccggg
     4681 aatggcaata tcagcgatct gctgagaacc aaccctgcgg tacgcatgga ttcaacgcaa
     4741 agtacctcgt tgaaccaggg agatattcgc cctgagaaaa tctctattca cggtgcgtcg
     4801 ccctaccaga atgcctattt gattgacggt attagtgcaa ctaataacct gaacccagcg
     4861 aatgagtccg atgccagtag tgcaaccaat attagcggga tgtcacaggg gtattatctt
     4921 gatgtcagct tactggacaa tgtgacgctt tatgacagtt ttgtgccggt tgaatttggt
     4981 cgcttcaatg gcggggtaat tgatgcaaag atcaaacgct tcaacgctga tgatagcaag
     5041 gtgaaattgg gttatcgcac tacgcgtttg gactggttaa catcgcatat cgatgagaat
     5101 aacaagagcg catttaatca aggttcttca ggaagtacct atttctctcc agattttaaa
     5161 aagaactttt ataccttgtc gtttaatcag gagctggctg ataactttgg cgttaccgcc
     5221 ggtttatcgc gccgccagtc tgatatcacc cgcgcggatt atgtttcgaa tgacggcatt
     5281 gtcgccggtc gggcacagta taaaaacgtt atcgatactg cattgagcaa atttacctgg
     5341 tttgccagcg accgctttac ccacgattta accttaaaat ataccggctc cagccgtgat
     5401 tataatacca gcaccttccc gcaatctgat cgcgaaatgg gtaataaatc ctatggtctg
     5461 gcatgggata tggatacgca actcgcatgg gccaaactac gtaccaccgt tggttgggat
     5521 catattagtg attatacccg tcacgatcat gacatctggt acaccgaact ttcatgtaca
     5581 tatggtgata ttacagggcg ttgtacccgt ggcggattag gacacatttc ccaggctgta
     5641 gataattaca ccttcaaaac acgcctggac tggcaaaaat tcgccgtggg tgatgtttcg
     5701 catcaaccct acttcggcgc ggaatacatc tattccgatg catggactga acgccataac
     5761 cagtctgaat cctatgtgat taatgctgca ggaaagaaaa ctaaccatac catttaccat
     5821 aaaggtaaag gcagcctggg aattgacaac tacacgctgt atatggcgga tcacattagc
     5881 tggcggaatg tgtcgttaat gcccggtgtg cgttatgact atgacaacta tctgtcaaac
     5941 cacaatatct ccccgcgctt tatgacggaa tgggatattt ttgctgatca aacctcaatg
     6001 attaccgccg gttataaccg ttactatggc gggaatattc ttgatatggg attacgtgat
     6061 atccgcaata gctggacgga atcggtatca ggtaataaaa ccctgacgcg ttatcagaat
     6121 ttgaaaacgc cttataacga tgaactggca atgggattgc agcaaaaaat cgataagaac
     6181 gttattgcac gcgcaaacta tgtttaccgt gaagcgcatg atcaaatcag caaaagcagt
     6241 cgtaccgaca gcgcgactaa aaccaccatt actgaatata acaacgatgg caaaaccaaa
     6301 acgcattcgt ttaacctcag ttttgaactg gccgaacccc tgcatatccg ccaggtagat
     6361 attaacccgc aaattgtctt tagctatatc aagagcaagg gcaacttgtc gttaaacaat
     6421 ggttatgagg agagcaatac cggtgataac caggtggttt ataacggtaa tctggtctct
     6481 tacgatagcg ttccagtggc agattttaat accccattaa agatctcctt aaacatggat
     6541 ttcacgcatc aaccgagcgg gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt
     6601 aaagctcgca ttatccttgg taagacaaat gcgcaataca tcagcgaata ttcagattac
     6661 aagcagtatg ttgacgaaaa actggatagc agcctgacct gggacacccg cttgtcctgg
     6721 acgccacaat ttctgaaaca acaaaacctg acgatcagtg ccgatattct caatgtactg
     6781 gatagcaaaa ccgctgttga tacaacgaac accggtgtgg cgacctacgc cagtggccgt
     6841 actttctggc ttgatgtcag catgaaattt taatagttcg gattttactt aaccgggtaa
     6901 attcgcccgg ttttcgcatg gagataatta tgagaaacct ctgtttctta ctgacgttag
     6961 tggcaactct gttgctcccc gggcggctga ttgccgccgc cttacagcag gatgaaaagt
     7021 taattaccgg gcaactggac aatggcttgc gatatatgat ttatccgcat gctcatccaa
     7081 aggatcaggt aaatttatgg ctgcaaattc ataccggttc attgcaggaa gaagacaatg
     7141 agcgcggcgt ggctcatttt gtagaacata tgatgtttaa cggcgcaaaa acatggccgg
     7201 gtaataaagt catcgaaaca tttgagtcaa tgggcctgcg ttttggtctc gatgttaatg
     7261 cctataccag ctatgacgaa acggtgtatc aggtgagttt gccgactacg cagaaacaaa
     7321 atctgcaaca agtgatggca atcttcagtg aatggagtaa tgccgcaacc tttgaaaaac
     7381 tcgaagtaga cgctgaacgt ggcgtaattt ctgaggaatg gcgtgcccat caggatgcga
     7441 aatggcgcac ctctcaggcg cgccgccctt tcctgctggc taatacccgt aatttagacc
     7501 gtgaacctat cggcctgatg gatactgtcg ccacggtcac accggcacaa ttgcgccaat
     7561 tttatcaacg ctggtatcaa ccaaataata tgacctttat cgtggtcggc gatatcgaca
     7621 gtaaagaagc gctggcgctg ataaaggata atttaagtaa gtttccggct aacaaagcag
     7681 ctgaaaatcg cgtctggccg acaaaagccg aaaaccacct gcgctttaat atcattaatg
     7741 ataaagaaaa ccgggtgaac ggcatcgcac tctattatcg cctgccaatg gtacaagtga
     7801 acgatgagca aagctttgtc gaacaagctg aatggagcat gttagttcag ctgttcaatc
     7861 aacgtctgca ggaacgcata cagtcgggcg agttgaagac tatttctggc ggcactgcgc
     7921 gcagcgttaa aattgcaccc gattatcagt cgctgttttt ccgtgtaaat gcacgagacg
     7981 ataatatgca ggatgctgcg aatgcattaa tggcagagtt ggcaaccatt gatcagcatg
     8041 gtttttctgc tgaagaactc gatgatgtca aatctacccg cctcacctgg ctgaaaaatg
     8101 cggttgatca gcaagctgaa cgtgatttac gtatgctgac cagtcgcctg gcatccagct
     8161 cattaaataa tacgccgttc ttgtcgccgg aagagacata tcaactttcg aaacgtctgt
     8221 ggcagcaaat taccgtgcaa agtctggcgg aaaaatggca gcagttaaga aagaaccagg
     8281 acgcattttg ggagcaaatg gtaaacaatg agcttgccgc caaaaaagca ttgtctcctg
     8341 cggctatcct ggcgctggaa aaagagtacg ccaacaaaaa gctggcggct tacatcttcc
     8401 caggcagaaa tttatcgtta acagtagacg ctgacccaca ggcggaaatt agcagcaaag
     8461 aaacgctggc tgagaatctg acatcattaa cactttccaa tggtgccaag gttattctgg
     8521 caaaatccgc gggtgaagag caaaagctac aaattattgc cgtatcgaat aaaggcgatt
     8581 taagtttccc tgcgcagcaa aaatcactta tcgcgctggc aaataaagca gttagcggaa
     8641 gcggcgttgg cgaactctcc tcttccagcc tgaaacgctg gagtgcggaa aactcggtaa
     8701 ccatgagcag taaagtcagt ggcatgaata cgttgctctc cgttagcgcg cggactaata
     8761 accctgaacc tggtttccag ttgattaacc agcgaatcac ccacagcacg attaacgaca
     8821 atatttgggc atcgctacaa aatgctcaaa ttcaggcgtt gaaaacgctc gaccagcgtc
     8881 cagcggagaa attcgcccag ctgatgtatg agacgcgcta tgctgatgac cgcacgaaat
     8941 tactgcaaga aaatcagatt gcacagttta ctgccgcaga tgcgctggct gccgatcgcc
     9001 agttgttttc atctccagcg gatatcacgt ttgtcattgt cggtaatgtc gcagaagaca
     9061 aactcgtggc gttaattacg cgttacttag gctcaatcaa acactctgat tcgccattag
     9121 ccgcaggtaa accattaact cgcgcgacgg acaacgcatc ggttactgta aaagaacaaa
     9181 atgaacctgt ggcacaggtt tcacagtgga agcgttatga ttcccggaca cctgttaatc
     9241 tggcgacgcg tatggcgctc gatgctttta acgtcgcact ggcaaaagat ctacgcgtta
     9301 atattcgtga acaggcatct ggggcataca gcgtttcttc tcgcctctcg gttgatcctc
     9361 aggctaaaga tatcagtcat ttgctggctt ttacttgtca accagaacga catgatgaac
     9421 agttaacgtt agcgaatgaa gtgatggtta agcgtttgac taaggggatc agtgagcaag
     9481 aactgaacga ataccagcaa aacgttcagc gcagcctcga tatccaacag cgtagcgttc
     9541 aacaattagc gaacactatt ataaatagtc ttattcaata tgacgatcct gcagcatgga
     9601 ctgagcagga gcaattgttg aaacaaatga cgatagagaa tgttaacact gccgttaaac
     9661 aatatctttc tcatccggta aatacttata ccggagtatt attgccaaaa taataacagc
     9721 cccgtcaaca catcgttggc ggggatttta gcaatattcg ctatttttat gtaataattt
     9781 tataaatgcg ttcaaaataa taaccaagta ctaatagtga tattttaatg tctgattttt
     9841 acgtgataat tcaggaggca cagaaagcgc ataaaaataa caacataaaa caccttgcca
     9901 ccacccaaaa attctctatt gcattgtttt taatgaaaaa aatatcattc caacaatatc
     9961 tcacgataaa taacattagg attttgttat ttaaacacga gtcctttgca cttgcttact
    10021 ttatcgataa atcctacttt tttaatgcga tccaatcatt ttaaggagtt taaaatggat
    10081 aagaagcaag taacggattt aaggtcggaa ctactcgatt cacgttttgg tgcgaagtct
    10141 atttccacta tcgcagaatc aaaacgtttt ccgctgcacg aaatgcgcga cgatgtcgca
    10201 tttcagatta tcaatgatga attatatctt gatggcaacg ctcgtcagaa cctggccact
    10261 ttctgccaga cctgggacga cgaaaacgtc cacaagttga tggatttatc cattaacaaa
    10321 aactggatcg acaaagaaga atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaa
//
LOCUS       DADLTF010000137        10256 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.138, whole genome shotgun sequence.
ACCESSION   DADLTF010000137 DADLTF010000000
VERSION     DADLTF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.138"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            80..883
                     /gene="hpaI"
                     /locus_tag="JN749_003320"
     CDS             80..883
                     /gene="hpaI"
                     /locus_tag="JN749_003320"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HAY7369625.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            905..2260
                     /gene="hpaX"
                     /locus_tag="JN749_003321"
     CDS             905..2260
                     /gene="hpaX"
                     /locus_tag="JN749_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HAY7369626.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2270..3160
                     /gene="hpaA"
                     /locus_tag="JN749_003322"
     CDS             2270..3160
                     /gene="hpaA"
                     /locus_tag="JN749_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HAY7369627.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3268..4124
                     /locus_tag="JN749_003323"
                     /pseudo
     CDS             3268..4124
                     /locus_tag="JN749_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4351..5909
                     /gene="hpaB"
                     /locus_tag="JN749_003324"
                     /pseudo
     CDS             4351..5909
                     /gene="hpaB"
                     /locus_tag="JN749_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5927..6439
                     /locus_tag="JN749_003325"
     CDS             5927..6439
                     /locus_tag="JN749_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HAY7369628.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6832..8982
                     /gene="btsT"
                     /locus_tag="JN749_003326"
     CDS             6832..8982
                     /gene="btsT"
                     /locus_tag="JN749_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HAY7369629.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            9032..9235
                     /locus_tag="JN749_003327"
     CDS             9032..9235
                     /locus_tag="JN749_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HAY7369630.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9246..10202
                     /gene="yjiA"
                     /locus_tag="JN749_003328"
     CDS             9246..10202
                     /gene="yjiA"
                     /locus_tag="JN749_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HAY7369631.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2445 a         2670 c         2650 g         2491 t
ORIGIN      
        1 agccccgaaa aagagccagt cagttgctta actgactggc attactccat tagctgccgc
       61 tttgtttaag gagagaacga tggaaaacag ttttaaagcg gcgctgaaag caggccgtcc
      121 gcagattgga ttatggctgg ggctgagcag cagctacagc gcggagttac tggccggacc
      181 aggattcgac tggttattga tcgacggtga acacgcgccg aataacgtgc aaaccgtgct
      241 cacccaacta caggcgattg cgccctaccc cagccagcca gtggtacgcc catcgtggaa
      301 cgatccggtg caaatcaaac aactgctgga cgtaggcaca caaaccttac tggtgccgat
      361 ggtacaaaac gccgacgaag cccgtaaagc ggtacgcgcc acccgttatc cccccgccgg
      421 tattcgcggt gtgggcagtg cgctggctcg cgcctcgcgc tggaatcgca ttcctgatta
      481 cctgcaaaaa gccaacgatc aaatgtgcgt gctggtgcag atcgaaacgc gtgaggcaat
      541 gaagaactta ccgcagattc tggacgtgga aggggtcgac ggcgtgttta tcggcccggc
      601 ggatctgagc gccgatatgg gttatgccgg taatccgcag cacccggaag tacaggccgc
      661 cattgagcag gcgatcgtgc agatccgcga agcgggcaaa gcgccgggga tcctgatcgc
      721 caatgagcaa ctggcaaaac gctatctgga actgggcgcg ctgtttgtcg ccgtcggcgt
      781 tgacaccacc ctgctcgccc gcgccgccga agcgctggca gcacggtttg gtaatagttc
      841 ttctatttcg atggcgaagc aaaacaacaa ctctgtctac taaacgtacg atgtggagcg
      901 catcatgacg acctctaccc tgcaatataa agataataaa actgttgaaa ttgaacatcg
      961 tgtcattaat aaattatttc gtcgcctaat catttttctt tttattctgt ttgttttttc
     1021 gtttcttgat cgcatcaata taggatttgc cggactgaca atgggcaagg atcttggcct
     1081 gacgtccaca atgtttggcc tcgccgcgac actcttttat gtcacgtacg tcatgtgcgg
     1141 gatccccagt aatatgatgt tagcgaaaat tggcgctcgt cgatggatcg ctgggatcat
     1201 ggtggtctgg ggaattgctt ccacctgcac aatgtttgca acaagccccg aaacactcta
     1261 tctactgcgt atgctggttg gtattgctga ggcgggtttt ttacctggta ttttggttta
     1321 tctgacctgg tggttcccgg catatcatcg ggccagagcc aatgccttat ttatgatagc
     1381 gatgccagtc actatgatgc tgggattgat cctttcaggc tacattctgg ccttggatgg
     1441 actgtggaat ctaaaaggct ggcaatggct ctttttgctg gaaggtctgc catcggtcat
     1501 tcttggtgtc gtcacctggt tttatctcaa cgacacacca gaccaggcga cctggcttga
     1561 tgaaaacgaa aaacaggctt taaaaacgat gattctccgt gaacaagaga gtgcatttgc
     1621 ccatgcatct gttccacgct caaccttacg tgaagtcctg accccagcca tcatgctata
     1681 tacacttgct tatttttgcc tgactaatac tctgagtgcg attaatatct ggacgccgca
     1741 aatcctacag agtttcaata ctggcagtag caacatcatg attggtttgc tggcggccat
     1801 cccccaattc tgcacaattt ttggcatgat ctggtggagt cgtcgttccg accgtcttaa
     1861 agaacgcaaa aagcatacga ttcttcccta cctgtttgcc gctgccggat ggttactcgc
     1921 ttctgcgaca gatcacagcc tcattcaact gagcggcatc attatggcct caatgggatc
     1981 atttaccgcg atgactatct tttggacaac acaggatcaa gttataagct tgcaatcgcg
     2041 tgccgttgcg atggctgtca tcaatgctgt cggcaatgta ggttcagcag tcagtccatt
     2101 acttatcggt attttgaaag atgcaaccgg cagttttagc tcaggtttgt ggtttgtagc
     2161 aggattgctg gtactgggcg cgttagtcat gacccgaatc cctatgggga gtcgggaaca
     2221 ctctctttca tctgaactta tcgcaccaaa accacaataa ggagtcatga tgtccagtga
     2281 agctatcttt gaacatggtt atgaaaatat tgatttggtc cgggaatatg atgcccgcta
     2341 cgctggtgaa gatgtgcatt atgaaacatt tacccggtta gcgaatttct ttggacgcga
     2401 tatgcgccct cactggcacg atagatattt tcaactccat tatctggtca ctggacgcat
     2461 tactttacaa ctggatgagc acttctattc tgtgcgcgca ccgctttttg tactaacacc
     2521 accatcggtg ccacatactt tctttactga tcctgatagc gatgggcatg ttctgaccgt
     2581 acgtcaggag ttaatttggc ccctggtaga aaagctctgg cctggtagtg gagatgccgc
     2641 aatgctacaa ggtatctgtc tgtcaccgga gggaatggaa catacactgg cggcattaaa
     2701 tcattattgg ccgataatat ccctggagtt tcaacaaaat aagccaggac gggaaattct
     2761 attgcaatca ctggcgcaaa gtatttttac aatcctactg cgtgaagcac caccaaatga
     2821 tatttcaaca tgcagcgtgc gcggcgagat gtgtttattc cagaagttta atcggctgat
     2881 tgacgaaaat tatcgccaac atttaatggt ccctgaatat gcaacaatgc tcggtatgag
     2941 tgaatcacgg cttaccgagc tgtgccgacg ttttgccaat caatcgccga agagattgat
     3001 tttcgaacga gtgagccggg aagccagacg gcttctgctc tatagcgagc aaagcatcaa
     3061 ccagatagcc cttgatcttg gctacaaaga tcccgcctat ttcgcccgtt ttttcaaccg
     3121 aatgatgggt tgctcaccaa cacaattccg tggtcgctga ccatttatca acctgagaga
     3181 ctcgacatga aaatatcttt acgctcacta tttgtggcgt gcctgattgc gccagtggca
     3241 ttcacctacg cctccgaacc gcaataaatg aaaattgtac tacttggaac gaaaggtggc
     3301 ccttctttat taaaagctga ccgtttaccg atggcaacgg cacttattat tggcaataaa
     3361 acctggttaa ttgatgctgg ttacggggcc agcttacaac tggtaaaaaa taatattcca
     3421 ctgcgccaga ttgataatat tttactcacc catttacaca gtgatcatat tctggactat
     3481 ccatcagtct taatgaatgc atgggctagc ggtctgcagg acaaaactat tcatgtattc
     3541 ggtccgccag gcacccgagc catgaccgct gccagttggc aggtcttcga tcgggacatc
     3601 acactgcgta tggaagaaga aggaaaaccc gatcctcgca aactcgttac agctcaagat
     3661 gttggtgaag gcgtagttta taaagatgat accgtgaccg tgactgcatt aaaggtccct
     3721 cattctcctt tccctgacgg tgaggctttt gcttatcgct tcgacacaca aggcaagcga
     3781 attgtcttct caggagatac ctcgtacttt cccccactgg caactttctc agaaggcgct
     3841 gatatacttg tgcatgaggc cgtacatgag ccctccgtcg ctaagctggc agacagtatt
     3901 ggcaatggta aaacactggc aaaagccatt gcttctcacc atacgactat tgaggatgta
     3961 ggcaaaattg ccaatgaagc aaaagtgaaa aaactcatcc ttagccacct ggtaccaatc
     4021 accgttccgg atgaaacatg gaagcaagaa gcacagaaaa ctattcagtc cctatcgtcg
     4081 ttggtcatga aaatatgacg attaccattc cattcgcccc ataaaaaatt ccgtccccgt
     4141 tagcccttgt tagcggggaa tattatgatt tgccttactg taagttgatc acgatcgcag
     4201 tttactattt aactccacct tgctgtcgaa aagtaccaac gttcgcctga accgtctctt
     4261 cccgacaacc gcgttcaacg cttcaattaa ataacaaaaa caacacacaa aattaacaat
     4321 cgtattccga ttaatactgt agaggtcgac atgaaaccag aagatttccg cgccagtacc
     4381 caacgtcctt tcaccgggga agagtatctg aaaagcctgc aggatggtcg cgagatctat
     4441 atctatggcg agcgagtgaa agacgtcacc actcatccgg catttcgtaa tgcggcagcg
     4501 tctgttgccc aactgtacga cgcgctgcac aaaccggaga tgcaggactc tctgtgttgg
     4561 aacaccgaca ccggcagcgg cggctatacc cataaattct tccgcgtggc gaaaagtgcc
     4621 gacgacctgc gccagcaacg cgacgccatc gctgaatggt cacgcctgag ctatggctgg
     4681 atgggccgta ccccagacta caaagccgct ttcggttgcg cactgggcgc gaatccgggc
     4741 ttttacggtc agttcgagca gaacgcccgt aactggtata cccgtattca ggaaactggc
     4801 ctctacttta accacgcgat tgttaaccca ccgatcgtca tttgccgacc gataaagtga
     4861 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga
     4921 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     4981 aagtgatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg
     5041 gcgtgaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccat
     5101 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     5161 tactgatccc atgggaaaac gtgctgatct accgcgattt tgatcgctgc cgtcgctgga
     5221 cgatggaagg cggtttcgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     5281 aactcgactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     5341 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     5401 tgagtgactc gatgtgttca gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     5461 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     5521 ttatagaacg caacgttacc agtggcctga tctacctccc ttccagtgcc cgtgacctga
     5581 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaac ggtatggatc
     5641 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gaatttggtg
     5701 gtcgtcacga gctgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcaat
     5761 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     5821 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     5881 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
     5941 acaacgcctg cgctttcatg acgcgatggt cagcctgtcg gcagcggtaa atattatcac
     6001 caccgagggc gacgccggac aatgcgggat tacggcaacg gctgtctgct cggtcacgga
     6061 tacaccaccg tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
     6121 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcgcgcca
     6181 cttcgcgggc atgacaggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
     6241 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gagagatccg
     6301 cgatgtgcag gcgattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
     6361 cagtgcagaa ggtcacggac ttatttactt taaacgccgt ttccatccgg tgatgctgga
     6421 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
     6481 tgtgatctca atcacatatc tacccgatga ataattcttg cccaaccgct caccgccatt
     6541 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcacgt
     6601 atctctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
     6661 ctagaacggc ttcggcctat gattaacaaa ttactctatg gattttggtt tgcaggaagg
     6721 cagcaagtga gtgaatcccc gggagcttac aaaagtaagt gtcagaggtg aacgaacgca
     6781 gctgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
     6841 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
     6901 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
     6961 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
     7021 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
     7081 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
     7141 ctggtgggac cggttctcgc cgcgcagatg ggctacctgc ctggcacgct gtggctgctg
     7201 gcgggggtag tgctggccgg tgcggtacag gactttatgg tgctgtttat ctcctctcgc
     7261 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tggggccagt accggggact
     7321 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgttct ggcgctgatt
     7381 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
     7441 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
     7501 tctgttattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
     7561 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
     7621 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
     7681 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
     7741 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
     7801 aaaggcgctc tattcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
     7861 cacgcgctga tctcttccgg tacgacgccg aaactgctgg ctaacgaaac cgacgcgcgt
     7921 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
     7981 gcgtccatca tcgaaccggg tctttacttc gcgatgaaca ccccgcctgc tggccttggc
     8041 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
     8101 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
     8161 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
     8221 ggtggtgcgc cgacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
     8281 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
     8341 gcgctggatg cgggtacccg ttctggccgc tttatgctgc aagacctgct gggtaacttc
     8401 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
     8461 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
     8521 agcctgtggc cgctgttcgg tatctccaac cagatgctgg cagccgtagc gctggtactg
     8581 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
     8641 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
     8701 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
     8761 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
     8821 aacgcaggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
     8881 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
     8941 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtgttt agccccgctt
     9001 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
     9061 atatctcggc caggcggcga agatgttgat tggtattcca gactacgaca actacgtcga
     9121 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
     9181 acgccagaac gcgcgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
     9241 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacta
     9301 ccctgctgcg ccatattctt aacgaacaac acggctacaa gattgccgtg attgaaaacg
     9361 aattcggcga agtctccgtt gatgatcaac tgattggcga ccgcgccacg cagatcaaaa
     9421 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcactactcg
     9481 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtg attgaatgca
     9541 ccggcatggc cgatcccggc ccgattattc agaccttttt ctcccatgag attttatgcc
     9601 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
     9661 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
     9721 aaaccgacgt cgcaggcgaa gcagagaaac tgcgtgaacg cctggcgcgc atcaacgccc
     9781 gcgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
     9841 acggttttat gctggaagag aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
     9901 ataaacaaaa cgatatttcg tcaattgtgg tggaactgga ttacccggta gatatcagcg
     9961 aagtttcccg cgtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    10021 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    10081 tctacagcgc cgactgggac aggccgtggg gcgatgaaaa accgcatagc acaatggtgt
    10141 ttatcgggat tcagttgccg gaggaagaga ttcgggcggc gtttgcggga ttgaggaagt
    10201 aagttcggga aagatcaact ttttgagagt tccaccgcaa cattattacg agtgga
//
LOCUS       DADLTF010000138        10166 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.139, whole genome shotgun sequence.
ACCESSION   DADLTF010000138 DADLTF010000000
VERSION     DADLTF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.139"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..367)
                     /locus_tag="JN749_003329"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="JN749_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(511..1134)
                     /locus_tag="JN749_003330"
     CDS             complement(511..1134)
                     /locus_tag="JN749_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HAY7369632.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVINH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(1131..2819)
                     /locus_tag="JN749_003331"
     CDS             complement(1131..2819)
                     /locus_tag="JN749_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HAY7369633.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAVGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(2968..5595)
                     /gene="gyrA"
                     /locus_tag="JN749_003332"
     CDS             complement(2968..5595)
                     /gene="gyrA"
                     /locus_tag="JN749_003332"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HAY7369634.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5742..6464
                     /locus_tag="JN749_003333"
     CDS             5742..6464
                     /locus_tag="JN749_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HAY7369635.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(6592..10166)
                     /gene="yfaL"
                     /locus_tag="JN749_003334"
                     /pseudo
     CDS             complement(6592..10166)
                     /gene="yfaL"
                     /locus_tag="JN749_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2361 a         2826 c         2478 g         2501 t
ORIGIN      
        1 actgttccac caagggatat tttttcagcg gggaaaactg gttgttctgt acataacggg
       61 aaggtttaat aatggcattg ccgagatgta attcgggtaa tgcctgagtc acattctgcc
      121 gtgactgaga agagaaagta cgctgcatca cgcggcgaga tttgccgtac cagttatccc
      181 acagctaggt cagcgtattg ttcagcccgt cgcccagata ttgcggttgc accacaatgc
      241 gatgcaggtt tttctgctgg cgcaaaaatg ccatcgggtc aggaatacga tacaggcgaa
      301 cgtccacgcc gccgtactct tccatctgat aacgccgata atcacgcccc ggcgcttcca
      361 gtcgcacttt cgcctcttca ctgctgctaa aactgctgtc agcaagcaaa aagaatgttc
      421 ccccggcggg cggcgcatag ttactggaag gaagcgaatc atcagcttta gcaagccctg
      481 ttcccacaag agacagggac aagcacgctg ctatcgcgcc agaaaattta aacgataaat
      541 gccaatgaaa ttgggattgg aatcgttggg tatccatcgg gtgtccttcc atgtcataag
      601 ttgttgcaga ctgactgcgc gcattccgtt gtcagttttc gtggcgcttc cggtgtggtt
      661 gatgacgtaa cgccccatcc agaccattaa gtgctgggca tcgccctgat cgaaaaaaat
      721 catatcgcca ggcagcgcct ggtttatgtc ttggccaata aactggctgt tgtactgaat
      781 caaattaatc gcggtcacgt aggggccgtt tttcccgctc ccctgattcc agttttgcgc
      841 cagttgacgc tgttcaggtg ttagcgtcat ctctggcggc aaatactggc tagataaacc
      901 gttacttttt agccatttac tgtcgtgaac tttcagcgtc tcgttcgccg caaatcgcac
      961 caggcccgca caatcctgct gataccagcg tggacttggc ccctggcgga gctgttcttg
     1021 tgcaatgcgt acaaaccagg cgcgaaacag cctggattgt tcgacattca gcatttcgct
     1081 atgggcaaca acacaacaca gccagcaaat cagcgccagc agcccgtgtc tcatagcggc
     1141 tgccaggtta tgggtagcca ctgccaggcg acaccgggtt ccatctgggc cagcttcatg
     1201 acataacgcg gttgttgaga taaagcgtcc agcttcggca ttaataaagt ttgtgcggcg
     1261 ttataaaaaa ccggttcgag attcttcggc agactggtca acgtttcgtt acgcagcagt
     1321 ttcgccacgc cttgtggatt gatatagagc ggaacgatgc catcagtggg tattacatcc
     1381 accattgccg ggcgggtttt attcagtgtt tgcagggcgt tattaaccag cgtgtcatcg
     1441 agggagaaaa gcagcgtttt gttttgcatc gccagcgaca cacggaaaaa ataatccgac
     1501 attaattgat cgggttgcgc cgcctgcgct ttcggatact gaccgtatcg ggaactcact
     1561 tcgcgacacc agatttgcgc ttcgccctgc tgagtctggc ttaccggcaa aacaccttct
     1621 ggcgctttgc tttcgtgcgc accaatattt tgcgtaaaca gtttccctgg caattgcgcc
     1681 tgttcggcag tgccatcaaa ctgaccgaca aacagcgggg tttgcaattt tgagtcttca
     1741 taccagcaca gccccacggc accgtctaac gccccattca gcttgtcgtt ttcctggctg
     1801 atgtgcgaaa gcatctcttc ggcaataccg tgtgaatacg gcaccgccac acagaagctg
     1861 gctccggcag gcatactgtt ccagaccggc gtaaaatcga aactggcatc tacgctggcg
     1921 gattcatcat ttaacgccac aaaactgtgc cagccgtcgt tacccatttc gaagcgtacg
     1981 ccagcaaaag aaggcattaa ccgctggtag ccaaaaccca gcaacctggc gctggccacg
     2041 atgcgcaggc gtactggcgt tttttcggct gtacgctctt ccaggccaaa gctggcttgc
     2101 cagcgttttt taccgctcaa caaatcactt gcgatcgccg tggcttcggt atcctgctga
     2161 tcatctttaa acaacatatc cgtactggaa aacaccagca tcttgtcctg atatgtcgcg
     2221 aacatcaggg cgttattgcc gttatagcgc aactgataaa caggaatagt ttcactattt
     2281 atcttgatgc tactgatttc cgttttgctt aactggctgt cgctggtagc ggcaaacaac
     2341 aatggctcca gcagtttgct taaaccactg cgctggatca gcaccatgta atgtgaaaga
     2401 tggccttgtt tatcgtgcca tagcgccgcc tgcgcgggct gatctaagag tgacgaaaag
     2461 agcttatctt tcagcgtgag atcgtgttca tagacaatgc ggcgaatgct gccttcaatg
     2521 cccagacgat ctgcatgatt ctgataatag aaaacgaaat cctcgctcag aacatcgtgg
     2581 agaaacggaa tggtgaggag atctttggga agctggctca gagagtcgct gtctagaaag
     2641 aggtccggct cattgagatc gatttgcaga ttgttgtgca ccaccagcgg cgacaacgtt
     2701 ttttctggcc cactgccagc atattgcaac gcccagacgc cagcggaaag cagtgctatt
     2761 gcgccaaaac ctacaagacc atagaaccgc cagcctttcg cctttttttc accactcatt
     2821 gccacattcc ttgtgtatag ccagccattt tttacgggca cagccaaact ttaccgcgcc
     2881 ctaatacgac aaaagcccag actttgcagc ctggactttt caattcaaac aagggagata
     2941 gctccctttt ggcatgaaga agtaaaatta ttcttcttct ggctcgtcgt caacgtccac
     3001 ttccggagca atttcatcat ccccttccgc ggcactgccg tcgatggtat ccagatcttc
     3061 ctcgtcaacc ggttcagcaa cacgctgcag acccactacg ttttcatctt ccgcagtacg
     3121 gatgaggatc acgccctggg tgttacggcc cacgatgctg atttctgaaa cgcgagtacg
     3181 taccagcgta ccggcatcgg tgatcatcat gatctggtcg cagtcatcta cctgcaccgc
     3241 gccaaccact aaaccgttac gttcggtaac cttgatggag ataacccctt tcgtcgcacg
     3301 cgacttggtt gggtattccg ccactgcagt acgtttaccg taaccgtttt gcgttgcggt
     3361 gaggattgcg ccatcgccac gaggcacgat cagagagacg actttatcgc cttcacctaa
     3421 gcgaataccg cgcacaccag tggtattgca gcccatcgca cggacagaag actctttaaa
     3481 gcgcaccact ttaccttcgg cggagaacag cattacttca tcttcgccac tggtcagatc
     3541 aacgccgatc agctcatcgc cgtcaaccag tttgatcgcc actttaccgg cggtacgcag
     3601 acggttgaat tcggtgagga cggttttctt cacagtaccg ttagcggtcg ccatgaagac
     3661 tttcacgcct tcttcaaact cggtcaccgg caggatcgcg gtgatacgtt cgtcctgttc
     3721 cagcggtagc aggttgacga tcggacgacc gcgcgcgcca cgagtggctt ccggcaactg
     3781 gtaaactttc atcgaataga cgcgaccacg gctggagaag cacagaatat ggtcgtgcgt
     3841 gttcgccacc agcagtcggt cgataaagtc ttcttcttta atacgtgcgg cagatttacc
     3901 tttcccgcca cgacgctgcg cttcgtattc agaaagcggc tgatatttaa cgtagccctg
     3961 gtgagagagc gtcacgacca catcttcctg ggtgatcaga tcttccaggt tgatgtctgc
     4021 gctgttggcg gtgatttcag tacgacgttt gtcaccgaac tgttcacgaa ccagctccag
     4081 ctcttcgcgg atcacttcca tcagacgatc ggcgctacca agaatacgca acagttccgc
     4141 gatctgatcc agcagctctt tgtattcgtc gagcagtttt tcgtgctcaa ggccggtcag
     4201 tttctgcaaa cgcagatcca gaatcgcctg agcttgctgt tcggtcaggt agtacagacc
     4261 atcacgcacg ccgaactctg gctccagcca ttccggacgc gcagcatcgt cgccagcacg
     4321 ttccaacatg gcggcaacgt tgcccagctg ccacggatta gcaaccagcg cagttttcgc
     4381 ttctgcaggc gtcggcgcat gacggatcag ttcgatgatc gggtcaatgt tcgccagcgc
     4441 cacggctaat gcttcaagga tatgagcacg atcgcgagct ttacgcagtt cgaaaatagt
     4501 acgacgggtc accacttcac ggcggtgacg aacaaacgcc gcgatgatgt ctttcaggtt
     4561 catgatcttc ggctgaccat ggtgcaatgc caccatgttg ataccgaaag aaacctgcaa
     4621 ctgggtctgg gagtagaggt tgttgagcac aacttcaccg accgcatcgc gtttcacttc
     4681 aatcacgatg cgcataccgt ctttgtcaga ctcgtcacgc agcgcgctga tgccttccac
     4741 gcgtttttct tttaccagtt ccgcaatctt ctcgatcagg cgcgctttgt ttacctgata
     4801 cggaatttcg tggacgataa tggtttcacg tccggttttg gcgtcaactt ccacttcagc
     4861 acgggcgcgg atatatacct tgccgcgacc ggtacggtaa gcttcttcaa taccgcgacg
     4921 accgttaatg attgccgccg tcgggaagtc cggccccggg atgtgttcca tcagcccttc
     4981 aatgctgatg tcttcatcat cgatatacgc cagacaaccg ttgatgactt ccgtcaggtt
     5041 gtgcggcggg atgttggttg ccatacctac ggcgataccg gaagaaccgt tcaccagcag
     5101 gttaggaatt ttggttggca tgacgtcggg aattttttcc gtgccgtcat agttatcaac
     5161 gaaatcgacc gtctcttttt cgagatcggc catcagttca tgggcaattt tcgccagacg
     5221 gatttccgta taacgcattg ccgccgcaga gtcgccgtcg atggaaccga agttaccctg
     5281 accgtctacc agcatgtaac gcagcgagaa tggctgcgcc atacggacga tcgtgtcata
     5341 aaccgccaag tcaccatggg gatggtattt accgattacg tcaccaacga cacgggcaga
     5401 ttttttatag gctttgttcc agtcattgcc tagtacgttc atggcgtaaa gtacgcgacg
     5461 gtgtaccggc ttcaggccat ctcggacatc tggcagcgca cggccaacaa tgaccgacat
     5521 cgcataatcc agataggagc tctttagctc ttcctcaatg ttgaccggtg taatttctct
     5581 cgcaaggtcg ctcatctaac cgctatccct ctactgtatc ccggattcaa aggtcgcaaa
     5641 ttataacaca gccgcgcagt ttgaggtaaa cctatacgct ttattcacat ccaatgcctg
     5701 atatactcgt ttgtcttgcc aattacggag tagaagtgcc aatgaatgcc gaaaaatcgc
     5761 cggaaaacca taacgtagac catgaagaga tcgctaaatt tgaagccgtc gcctcccgct
     5821 ggtgggatct tgaaggtgag ttcaaaccgt tgcaccgcat taacccgctg cgtctgggct
     5881 atattgccga gcgtgctggc ggtttatttg gcaaaaaggt gctcgatgtc ggttgtggcg
     5941 gcggcattct ggccgagagt atggcgcgcg aaggcgcgac ggtgaccggt ctggatatgg
     6001 gctttgagcc attgcaggtg gcaaagctgc acgcgctgga aagcggcatt caggtggatt
     6061 acgtgcagga aaccgtggaa gagcacgcgg caaaacatgc cgggcagtat gatgtggtga
     6121 cctgcatgga gatgctggag cacgtacccg atccgcagtc agtggtcaga gcctgtgcgc
     6181 aactggtgaa accaggcggc gatgtctttt tctcgacgct taaccgcaac ggcaagtcat
     6241 ggctgatggc ggtggttggc gcggaatata ttttgcgcat ggtgcccaaa ggtacgcatg
     6301 atgtgaagaa gtttattaaa ccggcagagc tgcttggctg ggtggatcag accagtttaa
     6361 aagagcgaca tataaccggg ctacattaca acccgatcac taattccttt aagctcggcc
     6421 ccggcgtgga tgtgaactat atgctgcaca cgcagaataa gtgaggttga tgtttggccg
     6481 cgccaatgcc tgatgcgacg cttgcagcgt cttatcaggc ctacaaatgc tctccgtagg
     6541 ccagataagg cgtttacgcc gcatccggca attgtgatgg tcagacagcg attaccattt
     6601 caccgtcatc gacaaaaacc ctgccgtctg ggcaaaatca tcactccctt tctgccacgc
     6661 cacgctgccg cgcagagaca ctcgctgact gatattgccc gtgactccca cttttatttc
     6721 accccgtagc ttcaccgcat cgtcactgat agtgctgcca tcttcttcaa tttccgtcgc
     6781 atggggatcg tgataataat tcagatctaa tgttggtgtg acaccaaccg cggtacgcca
     6841 ttcgctgtgt aaacccagcc gcgtctgaat atcatccccc tgcgattgtg acacgcgcgc
     6901 atggttagcg gcggtaaaat catcctgctg cacgccctga taaatcacct gcgcctgcgg
     6961 ttcaatcacc acaccacgcc ccggtaacca ctgatacccg gcttccagcg aggcgataat
     7021 ccccgacgaa tggtaacgat ccacgccatc ttcatgttcg gaaacatcat tgttaaacca
     7081 cgcgtattgc agccagctat ccagccaggc cccctgcttc tgattaccgt gctgatacca
     7141 gcttgaggtc agcccaacgg cataaccgtg gttctggtta tcggcgcgag ttccggtcat
     7201 attcgagcgg ctgtcgccct gattatcact gtagccacca accgccccaa gcatccactc
     7261 gccatccgtg ccccagcacc cgctaaacaa gccgccgcta agcagcaccg tagaagtgtc
     7321 ttcctgctga gccagttgcc ctgccgccgt gtaatgataa cgtccgccga taacacgtaa
     7381 attcagcgtc tgaccatcgc caccagcgtg atcgcgtcgc tccatcataa acgcctgatt
     7441 tgccgcccgc aggttattga gataaccgcc aactttggca ttcagcaccg gctggtaagc
     7501 aggcgtaggg gccgggtcgg gggttgggtc aggatccggc gtgggatcag gatcgggagt
     7561 cgggtctggc tcaggtggcg atggcggcgt tacttcttgc gatcgcagat accagtcgtt
     7621 gttatcttcc actagcgtgt agtcatacgc tcccatgttg acgtagccac tgccagccag
     7681 actgaactgt gcattgtttt gaaactgcgt gggatcagct gcgaaatcaa ccactttaat
     7741 gcctgtcgat gtcggctcac caatccctgt aatggagtta accaccacag ttgtgttgcc
     7801 agcagtatta ccgttcatca ccaattgatc gcttaccgag tcatcgccgt ttaattcgct
     7861 gtcgaatagt agcgtaccgc cgccggtata atcaccgttt accgtcagcg tatcgccagc
     7921 gacgccattt tgcaggctaa ccgcaccgga cgtattggtc agatcaccat taaccgtcac
     7981 gtcagcaaga taggtcaggc tgtcagtttc aaggttggta ttaagctcgc ctgtcaacgt
     8041 cacgccatcg gtagcattta ccagcgtccc atcgccgtta aatagtgagg cattaagggc
     8101 gacggaagta tcctgcccgg tcagacgcaa aaccgtacca tcgctgatgt cgatagtgcc
     8161 gctggaagta gcatcaattg actgaatatc ctgatccgcg ccagtaacga acgttgcccc
     8221 atcaccaacc cacagtgacg aagcataagg aaagatatcc gcaacatcgc ctttcagggt
     8281 gccttcttcg acacgtaccg ccgactgcgt tgtaccgctg gcggtcagct ccagcgtacc
     8341 cgcccccgtt ttagtaaatg tgctaccctc atcctgatgc tgcccgctgc tgtcagccat
     8401 cagtgcgccc cactgcgtgt ctacccccgc atcaactgcc acttcaccgt cggcgcgcat
     8461 ttcaatatca cgcccatgct cagtcgtcgt agagcgagta tcgacattgg cagtaatctc
     8521 catcacgctt tcttgttact tatcggtaaa gataacctgg cggttatagt gggtatctcc
     8581 aagctgcgag ttgtcgctca ccatcagcgt accggaggcg atttgtgttg tgcccaaata
     8641 actattattg tttgccagac ttacctgccc gtcgctcgca tcgaccacca cgtagacgcc
     8701 atctttcccg tcaccgccga tatgcaaatt gtcgccacct gcaagatcta ccacatcctg
     8761 actgccgata accgttccgc tgctgccact gacttcaagg ccatcattgt atgatgtgcc
     8821 gctctgccaa gtggagaaat cagagagatc gagcacaccg ccgtttaaca cgatcgactg
     8881 cggatcgtcc tggagagcgg taagatctgc cgcgtcgcct tccagcgaaa gcaccgcacc
     8941 atcatcaacg actatatctc cggttagcgc catcgactgc gcccctgcca gcacgtagct
     9001 gccgttttgc gcaatggtga gctgaccagc accttcgatg atgccagcaa aactgccctg
     9061 attaacagta acgttgccac cagcatcgat atttaaagtg ccattctgaa agcccgtcaa
     9121 tgcgtgcaca aaagtttgtt gggtcgagcc aacgtttagc tcagcctgat tctgatactg
     9181 atcaatactc cctatcgtca gaccgtagca gtcttgcgga tcgtcctggc aatgcgtatc
     9241 gccgacattc atcagggagt tgctgcgacc cagggtaact tcaccgtttt caatctgcat
     9301 ctcaccagta aagtcattgt tatctgcatt aagtaccaga tcgccggaac ctgttttggt
     9361 gattaacccg gtaccagcaa tagagtcaac agctccgtca ttctctgtat tgccgataac
     9421 cagcgttttt ccgtcggcaa tatcaaaggt aacttcactt aagccgagat acataaagcc
     9481 acccgccgca gtggaaggac catctccata gcctgctgcg ctattgttct catcgactaa
     9541 cacgccgccg ttctggctgt agctgtcatc aacagaaata tcaataagat agggagccct
     9601 cacgctattg gtatatatcg ccccgccata accttcggct gtgttatttg taaaggcggt
     9661 gttatttatt atcgtataac ctgaaggatg cttgctgtcg ctattattat cggtaacatc
     9721 gattgccccg ccatcgccat cactgtaact tgttgatgta tatgcctggt tattgttaaa
     9781 aacatcatca cttaaataga cgtcattatt aatggtataa attgcgccac ctttgccatc
     9841 attagcgata ttattgcgaa acatggcgtt agtgacacgt aaatcgacgg caccagtatc
     9901 attagtacca gaagaataga ttgcgccact atagccgcct gcgacgttac cggaaaaaat
     9961 aacatcagta agattcagcg ttgagttttc tttagcaaat atggccccgc cgttattata
    10021 ttctcctgta acggtgttat tggcaaacag agtcattcca gtttcatttt ctggtaatag
    10081 tgaaaattcc gctccttgtt gcaaaaatac ggccccaccg ctggcattat tggtcatatc
    10141 cgaaaaaacc agccattgcc catcgg
//
LOCUS       DADLTF010000139         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.140, whole genome shotgun sequence.
ACCESSION   DADLTF010000139 DADLTF010000000
VERSION     DADLTF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.140"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..336)
                     /locus_tag="JN749_003335"
     CDS             complement(<1..336)
                     /locus_tag="JN749_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7369636.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(364..926)
                     /locus_tag="JN749_003336"
                     /pseudo
     CDS             complement(364..926)
                     /locus_tag="JN749_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1130..1336)
                     /locus_tag="JN749_003337"
     CDS             complement(1130..1336)
                     /locus_tag="JN749_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7369637.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1443..2006)
                     /locus_tag="JN749_003338"
     CDS             complement(1443..2006)
                     /locus_tag="JN749_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HAY7369638.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3381..5216)
                     /locus_tag="JN749_003339"
     CDS             complement(3381..5216)
                     /locus_tag="JN749_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369639.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5317..5604
                     /locus_tag="JN749_003340"
     CDS             5317..5604
                     /locus_tag="JN749_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HAY7369640.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5576..7105
                     /locus_tag="JN749_003341"
     CDS             5576..7105
                     /locus_tag="JN749_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HAY7369641.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7275..8492)
                     /locus_tag="JN749_003342"
     CDS             complement(7275..8492)
                     /locus_tag="JN749_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7369642.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8859..9845)
                     /locus_tag="JN749_003343"
     CDS             complement(8859..9845)
                     /locus_tag="JN749_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369643.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
BASE COUNT         3138 a         2262 c         1846 g         2709 t
ORIGIN      
        1 acaccagaca tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac
       61 agcttcccta ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg
      121 cttacactgc aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag
      181 agaaggaatt gccttattca taccagggca aggtattaat ttaaactgta ataatttaat
      241 tttaggatgt gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc
      301 gttacaggaa agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat
      361 aggtcaaaaa ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt
      421 caaataatcc agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat
      481 atccaggatg taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc
      541 caaccctaag gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc
      601 atagtttccc agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat
      661 ttaaaggact gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt
      721 ttgccttgtt gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg
      781 aaacgagata tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca
      841 atcacaggag ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata
      901 ttcgtattga tagttctcag agatatatca cactctagat attttaatta agtatcagtt
      961 cgatatcggc atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac
     1021 tgacggtgag cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat
     1081 tcgttagcca ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg
     1141 cccatacatc atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg
     1201 ataatacgaa aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag
     1261 cttcaagtaa tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt
     1321 cagatttata aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga
     1381 aattcgtgtg aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata
     1441 attcagttat gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga
     1501 ataataccgt gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg
     1561 tctaccgcag atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct
     1621 ttagcccggc acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc
     1681 agttgcctgg atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa
     1741 tgaatatgaa gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc
     1801 gtctgggttt cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc
     1861 ccgaaactgg gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa
     1921 aaacgttgaa acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt
     1981 ttgtaattgg gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag
     2041 agagaattac gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag
     2101 taaaccagca gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga
     2161 ggtaatactt cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg
     2221 gaaaaacaca cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt
     2281 aatctgacat tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg
     2341 tcgaaccatt aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat
     2401 aatggtatac agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat
     2461 atttacctat acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac
     2521 gtaaccagtg ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc
     2581 cccatatgct tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt
     2641 agcataaaga ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac
     2701 aatacggcac agacacacaa taccataact ccaactaata atactaatta accttaatta
     2761 tttatggtat atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg
     2821 atcactgtat aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag
     2881 tgaccagtaa tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag
     2941 ccactaacat aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca
     3001 tgatttggcc caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag
     3061 agcttacacc gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca
     3121 atgaacatgc aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat
     3181 ggcgctaaga atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa
     3241 aacaaaacaa acaaataaat catactatta tagaattttc tcttgccaga catagataat
     3301 acccctgcta attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc
     3361 caaaacacac tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca
     3421 caaataatgg ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg
     3481 ccatacaaca tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat
     3541 acaccaagct tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct
     3601 atcatagttt ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc
     3661 agcgtcttac catcataaac atactcttta tctatcagaa tatttatctt atccagcacc
     3721 ttctctacgt catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg
     3781 atcttttcaa gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca
     3841 tcatactgta tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat
     3901 gcatcctgat aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt
     3961 tttctattta ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat
     4021 cctgaaggta attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca
     4081 tcattaattt tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg
     4141 gtcacatgaa cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat
     4201 gcgtcatcac aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata
     4261 tattcctgag atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg
     4321 ttttgaaact tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg
     4381 gtaaagtcat aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct
     4441 ctcaccaaca ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata
     4501 tcaagtaaga cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact
     4561 ttctccagtt catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg
     4621 atagccatga tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt
     4681 cctgtatatt tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat
     4741 cccagttcag ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta
     4801 tcagcgtgat caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa
     4861 aaagcagagt cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc
     4921 ccaaaagtgc gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga
     4981 taagaattca tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc
     5041 tgactgaaca ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag
     5101 aatttttgat gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc
     5161 ttaaacaacc ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc
     5221 tgtgctctcc ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa
     5281 ctgacacaac gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt
     5341 tacacgcctt cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga
     5401 tctactattt acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa
     5461 ccaataaggc ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg
     5521 atgttacata cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat
     5581 gacgacaaaa ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc
     5641 attacttgat gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat
     5701 gctgacggtc atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca
     5761 acgtaccagc cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc
     5821 tgcctgcgca aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata
     5881 tatcgacgat aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat
     5941 cctggagcgg agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg
     6001 agcagcactt gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat
     6061 cgtgaatgac gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct
     6121 gttgctgtct gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact
     6181 gtacaacaca ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt
     6241 ccggctgaat gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa
     6301 gttttttact ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt
     6361 ttgcgaaccc ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt
     6421 gacgcaacaa tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc
     6481 tctgagagca tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc
     6541 aaatgcagca tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca
     6601 gaatcctcat atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag
     6661 gatagcagca cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag
     6721 tactaatagc gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga
     6781 atcccttcgg gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat
     6841 cacaacagca ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct
     6901 caggaaaaaa tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag
     6961 gattgatgat aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa
     7021 aaaactaaat attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact
     7081 gaagtatcac cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt
     7141 attattcgtt ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga
     7201 actggcattg catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat
     7261 gccagtccat gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa
     7321 tccgcccatg cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc
     7381 gcctcaacct tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac
     7441 ccctgttcac tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc
     7501 cccggaatgt cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa
     7561 aggatctgct gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt
     7621 tccagaatgt aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt
     7681 ctttctgcag gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc
     7741 ctggcttcgc ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca
     7801 ttataaggtt catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc
     7861 attgcgggtt ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca
     7921 tcaacaggat ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg
     7981 cgctgtttaa cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct
     8041 gccacgtccg cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa
     8101 ttctcaagcg tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt
     8161 tctgcatgta cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca
     8221 gcctttttaa tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca
     8281 gtacgtgcag cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt
     8341 ttttgtgtca ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa
     8401 ggatgaaggg tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata
     8461 tgtcttgctt tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc
     8521 atccagtatg ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca
     8581 aaaagtgtaa tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt
     8641 atttcaggcg gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct
     8701 tttttttcgt atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa
     8761 attgtgcaca tcaactgtct ggttactcag tcaacaatca aaatgcgcaa gatagtacag
     8821 aacatatatg cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa
     8881 cggaaataca tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa
     8941 aaatagtaaa ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc
     9001 cattccacaa gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataaccagc
     9061 aatatgaata accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg
     9121 gagaaaaaca gatcgatttc ctgcaaaaaa aacctgccca gaaaagcgta ccgcttggag
     9181 aagcgcagaa aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt
     9241 atcgatttaa acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcaacggg
     9301 ctgatagtaa gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc
     9361 agacttttat attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg
     9421 cggatcatcc gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc
     9481 gcatataacg agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac
     9541 gacagaaatc ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt
     9601 ttcttaaacc acaaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg
     9661 agatcaaaaa taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc
     9721 tctgatggaa aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata
     9781 gcataaatgt atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga
     9841 atcatttgaa tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat
     9901 ccacgccagc aaaatcattt aacatattcc ccaaataggg cttattactt atttc
//
LOCUS       DADLTF010000140         9955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.141, whole genome shotgun sequence.
ACCESSION   DADLTF010000140 DADLTF010000000
VERSION     DADLTF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.141"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN749_003344"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="JN749_003344"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAY7369644.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN749_003345"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="JN749_003345"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HAY7369645.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="JN749_003346"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="JN749_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /locus_tag="JN749_003347"
     CDS             3456..5216
                     /locus_tag="JN749_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HAY7369646.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5622..6479
                     /gene="focA"
                     /locus_tag="JN749_003348"
     CDS             5622..6479
                     /gene="focA"
                     /locus_tag="JN749_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HAY7369647.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6534..8816
                     /gene="pflB"
                     /locus_tag="JN749_003349"
     CDS             6534..8816
                     /gene="pflB"
                     /locus_tag="JN749_003349"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAY7369648.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9008..9748
                     /gene="pflA"
                     /locus_tag="JN749_003350"
     CDS             9008..9748
                     /gene="pflA"
                     /locus_tag="JN749_003350"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HAY7369649.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2555 a         2495 c         2374 g         2531 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acatcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagagc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccagg
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcaacgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgccccg ctttacatat atttgcatta ataaaataat tgtaattata aggttaaata
     5341 tcggtaattt gtatttaata aatacgatcg atattgttac tttattcgcc tgatgctccc
     5401 ttttaattaa ctgttttagc ggaggatgcg gaaaaaattc aactcatttg ttaattttta
     5461 aaatttattt ttatttggat aatcaaatag ttactccgta tttgcataaa aaccatgcga
     5521 gttacgggcc tataagccag gcgagatatg atctatatca atttctcatc tataatgctt
     5581 tgttagtatc tcgtcgccga cttaataaag agagagttag tgtgaaagct gacaaccctt
     5641 ttgatctttt acttcctgct gcaatggcca aagtggccga agaggcgggt gtctataaag
     5701 caacgaaaca tccgcttaag actttctatc tggcgattac cgccggtgtt ttcatctcaa
     5761 tcgcattcgt cttctatatc acagcaacca ctggcacagg cacaatgccc ttcggcatgg
     5821 caaaactggt tggcggcatt tgcttctctc tggggctgat tctttgtgtt gtctgcggag
     5881 ccgatctctt tacttccaca gtgttgattg ttgttgctaa ggcgagtggg cgcatcacct
     5941 ggggtcagtt ggctaaaaac tggctaaatg tctattttgg caatctggtc ggcgcactgc
     6001 tgtttgtact tttaatgtgg ctttccggcg agtatatgac cgcaaatggt caatggggac
     6061 taaacgtcct acaaaccgcc gaccacaaag tgcaccatac ttttattgag gccgtctgtc
     6121 ttggtatcct ggcaaacctg atggtatgtc tggcagtatg gatgagttat tctggccgca
     6181 gcctgatgga caaagcgttc attatggtgc tgccggtcgc gatgtttgtt gccagcggtt
     6241 ttgagcacag tatcgcaaac atgtttatga tcccgatggg tattgtaatc cgcgacttcg
     6301 catctccgga attctggacc gctgtcggtt ctgcaccgga aaatttttct cacctgaccg
     6361 tgatgaactt catcactgat aacctgatcc cggttacgat cggtaatatt atcggcggtg
     6421 gtttgttggt tgggttgaca tactgggtca tttacctgcg tgaaaacgat caccattaat
     6481 ggttgtcgaa gtacgcagta aataaaaaat ccacttaaga aggtaggtgt tacatgtccg
     6541 agcttaatga aaagttagcc acagcctggg aaggttttac caaaggtgac tggcagaatg
     6601 aagtaaacgt ccgtgacttc attcagaaaa actacactcc gtacgagggt gacgagtcct
     6661 tcctggctgg cgctactgaa gcgaccacca ccctgtggga caaagtaatg gaaggcgtta
     6721 aactggaaaa ccgcactcac gcgccagttg attttgacac cgctgttgct tccaccatca
     6781 cctctcacga cgctggctac atcaataaag cgttggaaaa agttgttggt ctgcagactg
     6841 aagctccgct gaaacgtgct cttatcccgt tcggtggtat caaaatgatc gaaggttcct
     6901 gcaaagcgta caaccgcgaa ctggacccga tgatcaaaaa aatcttcact gaataccgta
     6961 aaactcacaa ccagggcgta ttcgacgttt acactccgga catcctgcgt tgccgtaaat
     7021 ccggtgttct gaccggtctg ccagatgctt atggccgtgg ccgtatcatc ggtgactacc
     7081 gtcgcgttgc gctgtacggt atcgactacc tgatgaaaga caaatacgct cagttcacct
     7141 ctctgcaggc tgatctggaa aacggcgtaa acctggaaca gactatccgt ctgcgcgaag
     7201 aaatcgctga acagcaccgc gctctgggtc agatgaaaga aatggctgcg aaatacggct
     7261 acgacatctc tggtccggca accaacgctc aggaagctat ccagtggact tacttcggct
     7321 acctggctgc tgttaagtct cagaacggtg ctgcaatgtc cttcggtcgt acctccacct
     7381 tcctggatgt gtacatcgaa cgtgacctga aagctggcaa gatcaccgaa caagaagcgc
     7441 aggaaatggt tgaccacctg gtcatgaaac tgcgtatggt tcgcttcctg cgtactccgg
     7501 aatacgatga actgttctct ggcgacccaa tctgggcaac cgaatctatc ggtggtatgg
     7561 gcctcgatgg tcgtaccctg gttaccaaaa acagcttccg tttcctgaac accctgtaca
     7621 ctatgggtcc gtctccggaa ccgaacatga ccattctgtg gtctgaaaaa ctgccgctga
     7681 acttcaagaa attcgccgct aaagtgtcca tcgacacctc ttctctgcag tatgagaacg
     7741 atgacctgat gcgtccggac ttcaacaacg atgactacgc tatcgcttgc tgcgtaagcc
     7801 cgatgatcgt tggtaaacaa atgcagttct tcggtgcgcg tgcaaacctg gcgaaaacca
     7861 tgctgtacgc aatcaacggc ggcgttgacg aaaaactgaa aatgcaggtt ggtccgaagt
     7921 ctgaaccgat caaaggcgat ctcctgagct acgatgaagt gatggagcgc atggatcact
     7981 tcatggactg gctggctaaa cagtacatca ctgcactgaa catcatccac tacatgcacg
     8041 acaagtacag ctacgaagcc tctctgatgg cgctgcacga ccgtgacgtt atccgcacca
     8101 tggcgtgtgg tatcgctggt ctgtccgttg ctgctgactc cctgtctgca atcaaatatg
     8161 cgaaagttaa accgattcgt gacgaagacg gtctggctat cgacttcgaa atcgaaggcg
     8221 aatacccgca gtttggtaac aatgatccgc gtgtagatga cctggctgtt gacctggtag
     8281 aacgtttcat gaagaaaatt cagaaactgc acacctaccg tgacgctatc ccgactcagt
     8341 ctgttctgac catcacttct aacgttgtgt atggtaagaa aactggtaac accccagacg
     8401 gtcgtcgtgc tggcgcgccg ttcggaccgg gtgctaaccc gatgcacggt cgtgaccaga
     8461 aaggtgctgt agcgtctctg acttccgttg ctaaactgcc gtttgcttac gctaaagatg
     8521 gtatctccta caccttctct atcgttccga acgcactggg taaagacgac gaagttcgta
     8581 agaccaacct ggctggtctg atggatggtt acttccacca cgaagcatcc atcgaaggtg
     8641 gtcagcacct gaacgttaac gtgatgaacc gtgaaatgct gctcgacgcg atggaaaacc
     8701 cggaaaaata tccgcagctg accatccgtg tatctggcta cgcagtacgt ttcaactcgc
     8761 tgactaaaga acagcagcag gacgttatta ctcgtacctt cactcaatct atgtaattag
     8821 atttgactga aatcgtacag taaaaagcgt acaataaagg ctccacgaaa gtggggcctt
     8881 ttttagcacg agagcctttt ttgtcagcta tctatacttt aaggtgactg ccaaaacaga
     8941 ctcgacgtag ccttcgagct gcgcaccaac acggcctcag atgggccaca tctggagaaa
     9001 caccgcaatg tcagttattg gtcgcattca ctcctttgaa tcctgtggaa ccgtagacgg
     9061 cccaggtatt cgctttatca cctttttcca gggctgcctg atgcgctgcc tgtattgtca
     9121 taaccgcgac acctgggaca cgcatggcgg taaagaagtt accgttgaag atttgatgaa
     9181 ggaagtggtg acctatcgcc actttatgaa cgcttccggc ggcggcgtta ccgcatccgg
     9241 cggtgaagca atcctgcaag ctgagtttgt tcgtgactgg ttccgcgcct gtaaaaaaga
     9301 aggcattcat acctgtctgg ataccaacgg ttttgttcgt cgttacgatc cggtgattga
     9361 tgaactgctg gaagtaaccg acctggtaat gctcgatctc aaacagatga acgacgagat
     9421 ccaccaaaat ctggttggag tttccaacca ccgcacgctg gagttcgcta aatatctggc
     9481 gaacaaaaat gtgaaggtgt ggatccgcta cgttgttgtc ccaggctggt ctgacgatga
     9541 cgattcggcg catcgcctcg gtgaatttac ccgtgatatg ggtaacgttg agaaaatcga
     9601 acttctcccc taccacgaac tgggcaaaca caaatgggtg gcgatgggtg aagaatacaa
     9661 actcgatggt gttaaaccac cgaagaaaga gaccatggaa cgcgtgaaag gcattcttga
     9721 gcagtacggt cataaggtaa tgttctaatt tccccgtaaa gcggcaactt tattgagttg
     9781 ccgctttttt attccgcgct tcgttctgct acagcgtttt taacacatct tatagtgcat
     9841 ctaccatatc ccgtacctga gataataacg actgataata gctcgcgtga agttcttccc
     9901 catcgaatac cactgtatta cacaagaaag tgacatcggc atcttcaata ccaag
//
LOCUS       DADLTF010000141         9772 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.142, whole genome shotgun sequence.
ACCESSION   DADLTF010000141 DADLTF010000000
VERSION     DADLTF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.142"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            49..579
                     /locus_tag="JN749_003351"
     CDS             49..579
                     /locus_tag="JN749_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7369650.1"
                     /translation="MSITGVNIGRLFREGFSNYDPIGVLNAMASQRTKEARGGELQIN
                     ELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGAAKMLFSHAQKTLAWDAFAFT
                     EVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAV
                     QEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="JN749_003352"
                     /pseudo
     CDS             597..1235
                     /locus_tag="JN749_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="JN749_003353"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="JN749_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HAY7369651.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN749_003354"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="JN749_003354"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HAY7369652.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="JN749_003355"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="JN749_003355"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HAY7369653.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN749_003356"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="JN749_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HAY7369654.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="JN749_003357"
     CDS             complement(5552..6613)
                     /locus_tag="JN749_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HAY7369655.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="JN749_003358"
     CDS             complement(6610..8007)
                     /locus_tag="JN749_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HAY7369656.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="JN749_003359"
     CDS             8163..9161
                     /locus_tag="JN749_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAY7369657.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..>9772)
                     /gene="ompG"
                     /locus_tag="JN749_003360"
     CDS             complement(9272..>9772)
                     /gene="ompG"
                     /locus_tag="JN749_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7369658.1"
                     /translation="RPDWDVKLTDDLRFNGWLSMYKFANDLNTTGYADTRVETETGLQ
                     YTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYLPLTLGNHSVTPYTRIGLDR
                     WSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYAFEWQDHDEGDSDKFHYAGV
                     GVNYSF"
BASE COUNT         2474 a         2598 c         2344 g         2356 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgaacaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcggcaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctagctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc ccacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca gcgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcggcttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccta ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgctgcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagcgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcaaactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcataggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtgccg
     5701 tcagaagtcc agctccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaggcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgtctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcgtc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgcc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagccggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gaatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag aaaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actccagcga tccagcccaa
     9481 tgcgcgtata cggcgtaacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggg cg
//
LOCUS       DADLTF010000142         9708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.143, whole genome shotgun sequence.
ACCESSION   DADLTF010000142 DADLTF010000000
VERSION     DADLTF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.143"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(329..673)
                     /locus_tag="JN749_003361"
     CDS             complement(329..673)
                     /locus_tag="JN749_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HAY7369659.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="JN749_003362"
     CDS             805..2715
                     /locus_tag="JN749_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAY7369660.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQLGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="JN749_003363"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="JN749_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAY7369661.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="JN749_003364"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="JN749_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HAY7369662.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRRQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="JN749_003365"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="JN749_003365"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HAY7369663.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="JN749_003366"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="JN749_003366"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HAY7369664.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNARQLRTRQLACLQLLAD
                     WRLRKARECDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JN749_003367"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="JN749_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HAY7369665.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8853)
                     /locus_tag="JN749_003368"
     CDS             complement(8251..8853)
                     /locus_tag="JN749_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-hydroxylating oxygenase subunit alpha"
                     /protein_id="HAY7369666.1"
                     /translation="MLEACPEVHNLKLAARFTTRTPANWKNIVDNYLECYHCGPAHLG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTNEQKSLIEWYRDVFHPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9210..>9708)
                     /locus_tag="JN749_003369"
     CDS             complement(9210..>9708)
                     /locus_tag="JN749_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7369667.1"
                     /translation="FAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFIQS
                     QSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALMPE
                     IFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEPQD
                     VKKAV"
BASE COUNT         2370 a         2368 c         2559 g         2411 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtagcagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc taggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg gtcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 agacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgatgagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgccaggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagtg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaactgca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 cggtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaac
//
LOCUS       DADLTF010000143         9678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.144, whole genome shotgun sequence.
ACCESSION   DADLTF010000143 DADLTF010000000
VERSION     DADLTF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.144"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            383..1501
                     /locus_tag="JN749_003370"
                     /pseudo
     CDS             383..1501
                     /locus_tag="JN749_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
     gene            1567..1815
                     /locus_tag="JN749_003371"
     CDS             1567..1815
                     /locus_tag="JN749_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HAY7369668.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLSAIEYFVLRFIWRRWFSLAD"
     gene            1880..2248
                     /locus_tag="JN749_003372"
     CDS             1880..2248
                     /locus_tag="JN749_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HAY7369669.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPRYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2342..2995
                     /gene="nfsB"
                     /locus_tag="JN749_003373"
     CDS             2342..2995
                     /gene="nfsB"
                     /locus_tag="JN749_003373"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HAY7369670.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3103..4350
                     /locus_tag="JN749_003374"
     CDS             3103..4350
                     /locus_tag="JN749_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAY7369671.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JN749_003375"
     CDS             complement(4418..5794)
                     /gene="pheP"
                     /locus_tag="JN749_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HAY7369672.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5895..9030)
                     /gene="cusA"
                     /locus_tag="JN749_003376"
                     /pseudo
     CDS             complement(5895..9030)
                     /gene="cusA"
                     /locus_tag="JN749_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9042..9583)
                     /locus_tag="JN749_003377"
                     /pseudo
     CDS             complement(9042..9583)
                     /locus_tag="JN749_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2377 a         2685 c         2337 g         2279 t
ORIGIN      
        1 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
       61 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      121 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      181 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      241 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      301 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      361 aacgtcaact taagagaaac ctatgccatt acccgatttt catttttctg aaccttttac
      421 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      481 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
      541 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
      601 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
      661 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
      721 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
      781 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
      841 cttgtcacgg tttgtgccgc attttattgc cctttccgcc gcgtcgccat atatgcaggg
      901 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
      961 cccgatgccg tgggtcagta actagcaaca atttgaagcc ctgtttcgct gtctgagtta
     1021 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1081 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtgaatat
     1141 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1201 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1261 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1321 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1381 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1441 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1501 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tatatttaac
     1561 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     1621 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     1681 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     1741 tttttagtgc tgagcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     1801 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     1861 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     1921 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     1981 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2041 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccaag
     2101 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2161 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2221 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2281 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2341 tatggatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2401 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2461 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     2521 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     2581 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     2641 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     2701 tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     2761 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     2821 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     2881 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     2941 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3001 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3061 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3121 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3181 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3241 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3301 aaactcttcc accgtttagc ttttaccctg cagggaatta tcgtcaatat tcaggcggta
     3361 ttctggctgc aaaaaggcac cgaagcggca gatattctga ctacctgcgc gcagttgtgg
     3421 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3481 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     3541 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     3601 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     3661 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     3721 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     3781 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     3841 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     3901 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     3961 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4021 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4081 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4141 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4201 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4261 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4321 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4381 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4441 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4501 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     4561 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     4621 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     4681 tcatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     4741 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     4801 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     4861 ccccgctgtt atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta
     4921 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     4981 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggatgc
     5041 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5101 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5161 tcagcccatt ccagccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5221 ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5281 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5341 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5401 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5461 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     5521 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     5581 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     5641 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg ccacccagcg
     5701 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     5761 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     5821 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     5881 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     5941 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggggcg
     6001 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6061 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6121 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6181 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6241 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6301 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6361 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6421 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6481 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6541 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6601 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     6661 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     6721 ggataacgca gattaattgg ataacgggca atcccttcca ccctttcgcc aaccatcgcc
     6781 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     6841 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc
     6901 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     6961 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7021 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7081 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7141 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7201 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7261 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7321 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7381 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7441 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7501 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     7561 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7621 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7681 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7741 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     7801 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     7861 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     7921 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg
     7981 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8041 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8101 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8161 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttaatgc cccggcgcat
     8221 ctccgggcca atctggacct tcgcaacatc gcgcagataa acgggcacgc cattttcact
     8281 ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgt agatagccgc tggcgcgcac
     8341 catatattct gcttccgcca gttcgattga cgaaccgccc gcttcctggt ttgaagcatc
     8401 cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct gaggatcgat
     8461 aaccacctga tactctttca ccacaccgcc taccgacgcc acttccgcaa cgtcaggaat
     8521 ggttttcagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg caagatcgtg
     8581 cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg
     8641 tcccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag
     8701 cacccgcgaa cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga
     8761 gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca acatggtggt
     8821 ggttagcgga taagtcacct gattttcaac gatttgcggt gcctggccgg gatagctggt
     8881 tttaataatc acctgcacat cggagagatc cggcaacgca tccactggcg tattaatgat
     8941 ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc
     9001 caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca
     9061 ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac
     9121 aggccgccgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacgcct
     9181 tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta
     9241 atcacccgct gttcgttgcc ggtatcaatc agcgcctcgt cggcgttgtc gacttccaga
     9301 cgcagctgca gcgtgcgggt cgcggcatcc acgccaggta gcagcgtcca tttgcggatg
     9361 gtgagtgttt tatccggtcg cgccggaacg gtgagggtaa actgcgaggc atctttcacc
     9421 agccaggcga tggactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt
     9481 cgcgattacg cgcaataccc ctgagaaccg ccatgaacaa gggtttcatg atttgtgatt
     9541 ttagtttagt acgcaatttg gtacacaaca ctattttcac ttcagcgggt agtcatcaaa
     9601 ctcaccataa cgagcatcgt tgatgatgta tgtgatcacc acaaatatca catctggact
     9661 agttccgtct tcatgcgc
//
LOCUS       DADLTF010000144         9497 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.145, whole genome shotgun sequence.
ACCESSION   DADLTF010000144 DADLTF010000000
VERSION     DADLTF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.145"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..399)
                     /gene="ompG"
                     /locus_tag="JN749_003378"
     CDS             complement(<1..399)
                     /gene="ompG"
                     /locus_tag="JN749_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HAY7369673.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRW"
     gene            complement(444..1526)
                     /locus_tag="JN749_003379"
     CDS             complement(444..1526)
                     /locus_tag="JN749_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7369674.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="JN749_003380"
     CDS             complement(1540..2199)
                     /gene="pgmB"
                     /locus_tag="JN749_003380"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAY7369675.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="JN749_003381"
     CDS             complement(2196..4463)
                     /gene="ycjT"
                     /locus_tag="JN749_003381"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HAY7369676.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     ARGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(4460..5066)
                     /locus_tag="JN749_003382"
                     /pseudo
     CDS             complement(4460..5066)
                     /locus_tag="JN749_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            complement(5114..5860)
                     /locus_tag="JN749_003383"
                     /pseudo
     CDS             complement(5114..5860)
                     /locus_tag="JN749_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
     gene            complement(5891..6733)
                     /locus_tag="JN749_003384"
     CDS             complement(5891..6733)
                     /locus_tag="JN749_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAY7369677.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVTWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(6720..7601)
                     /locus_tag="JN749_003385"
     CDS             complement(6720..7601)
                     /locus_tag="JN749_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAY7369678.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLLAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(7622..8905)
                     /locus_tag="JN749_003386"
                     /pseudo
     CDS             complement(7622..8905)
                     /locus_tag="JN749_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8919..>9497)
                     /locus_tag="JN749_003387"
     CDS             complement(8919..>9497)
                     /locus_tag="JN749_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAY7369679.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMDALSRRESSDEERCARFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         2266 a         2647 c         2324 g         2260 t
ORIGIN      
        1 ccagcgctgc atattcgccg tgtctttgcc cggttcatca acgtagtgat aaccataatt
       61 acggaaaccg ccggtcaggc cgaaactgaa atcatcgctt tcgaggaact gataatgcac
      121 ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat ctaccggccc
      181 ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa aatagactga
      241 aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta tttcgtacat
      301 cgcaccgata ttaaagtgcc agtcgttctt ttcctcggcc tgtgcgcagg ccattcccgc
      361 acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt gtattatcct
      421 aggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc atcaaagaaa
      481 tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata atcatttaac
      541 gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat aaattccgca
      601 ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg cgcgtccgga
      661 tgaatatctt ccgggcgtat tcccatcacg atgggcttat gcaaactttc ctgtgttttc
      721 agaaccgcta atttctcttc gagaatggtt aatttaagcg tttccgtaac gaatttatcg
      781 ccatcgatcg tgccgcgaat aaaattcatc gctggtgatc caataaatcc ggcaacaaac
      841 atattcgcgg gttggttata aacggttttc ggcgcaccta cttgctgaac aatcccgtct
      901 ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg ggtcacgtag
      961 atcatggtgg tgttcagttt ctgatgcagc ttgctgatct ctgcacgcat ttgtacgcgc
     1021 agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc ttcgcgcaca
     1081 atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc cggcttgcgt
     1141 ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc atcaatcacc
     1201 tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac cgtcatatgc
     1261 ggatacaacg cgtagttctg gaacaccatc gcgatattgc gtgctttggc tggaacgtca
     1321 ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag cccggcaatc
     1381 atgcgcaggg tggtcgattt accgcagccc gacgggccga caaatacgat gaactcttta
     1441 tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta gattttttga
     1501 atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt gccagaaggc
     1561 cgataaccgc gaccaggtca gggagtccgt tgagggcaac agtaattgcg ccccggttaa
     1621 gcccgcaccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa tgcccgcctg
     1681 cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac aggcagcgag
     1741 aaagatttcc ggatccggtt tcgagttttt aagttgggaa gcatccgcgc agaaggtgaa
     1801 aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga cggaagccag
     1861 ccctaccgga atctgctgtg cacggagctc tgccagcaaa ttgcgaatac cgggcagcac
     1921 agcattaacc gtcagctcgc gcagtgagtg gacgtaaagc agatttttgc gatacgccag
     1981 ttgcgcccgc tcctgcgggt taaagtcgcc ctctttgccc ccgtgttgca gaatgcgccg
     2041 cagagactca tcgcggctga tcccttttag agattcgtta aactgcgcat caatactgat
     2101 gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg tatcggtgat
     2161 tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg atgcgtggta
     2221 gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc ttcagccacg
     2281 gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat cgcaatacgc
     2341 tgcgcgtcga gagtgacctg taattcgcag ccctgccaga acagagggaa agacaactgt
     2401 tgccactgct caggtaacgc cggatttaga tgcaattcac cgtcacgcac gctcaccccg
     2461 gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg gataccatca
     2521 tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca gaactgataa
     2581 ctttgggtca gcaggccaca gcgtgcagca acaatgccgt ggattgcttt acttaatgac
     2641 gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga tgccgatgag
     2701 aactgctctg gcagcatgta attgagcatc accacatcag cttgtttgag gatctgcatc
     2761 tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc cgccgctttg
     2821 tatttcgcca gattaatcgc cggcttagtc ataaacgaat catcctgcgg caaaacaccg
     2881 tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcacg atgaataaac
     2941 gcgtcgtcac tacagccaaa ctggcgggca atactcagcg cctgctggac gttgtaatac
     3001 accatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc tggcccaata
     3061 acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca gaactttgca
     3121 gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc cgtggtctgc
     3181 cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc ctgcgccgag
     3241 gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg cgtctcttct
     3301 tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt gcgtcgcgct
     3361 ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg ggcaaccgtc
     3421 ggatcgctaa acagatggaa cggcagcaaa aatacttctg tatcccagaa aacgtggcct
     3481 ttgtagcctt cgccggttaa gccttttgcc gcaatactgc tgcgctcgcc gtgagccggc
     3541 gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcatcgccg
     3601 ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt tgatgctgca
     3661 agatgttggt cgtaactctg ttgcgcgcac atttcaagct ggcgaagcga cacgctgccc
     3721 cactcgtcta aagcagcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac
     3781 gtcactgtct cgcctgcatg aagctgcgca ctggtatgtt gcagtaaacg gcgctctttg
     3841 gcggtatagc attgctgcac atcaccgctc acctggcagc aacaactgat ggccacatca
     3901 ctgcgcccat cctgggtggt gtagatcccc tgcatcaaat gctgaccaaa cacccgcacc
     3961 tgggtttcgt cgagatgctg gcgaccgtgg ttggtttgcg tggcgtcaat gcctgttgaa
     4021 atcagcactg acgcgtcggc gtccagtggc ataatagtga tttccagcgc aatgagcggc
     4081 agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgcgcc gttgctggta
     4141 cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct
     4201 tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc
     4261 agattcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac
     4321 atccctcgcg cctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg
     4381 ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct
     4441 gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgatata
     4501 acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct
     4561 tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca
     4621 tttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa
     4681 acagcgtcgc accggcttta tcaccacaaa agctgacgtt cataatcaac tgttcgcgga
     4741 tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc
     4801 caaacagcga atcttcgacg ctgtaagttg ccggatccca ctcgccaaat tgaccacagc
     4861 tcttttgcgt gccgatcttt tgaaaactat gcgcattcac gcttttcacc gccggaaagc
     4921 ccagcacata catcgcagca tccagcatat gaatgccgat gtcgatccag ttctttattg
     4981 gtaaagacac cccagccggg aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa
     5041 atctctccca aaacgccgtt ggtcacctgt tcacgcagct gttgcgtatc gagcgcaaac
     5101 gatggtgaaa gtcgtaggcc aggccgcgca gcgccgattg cagcgcgtcg gcgtaaccgc
     5161 tggtttcgat aatcacatcg gccccctgct tgccggtcag cgttttgatc tctttgccta
     5221 cgtcagtgcc aatggggtta aggcagaaat cagcaccgtg gcgacgggca atatcacagc
     5281 gatgggcgat aggatcgacg ccaattacca ccgatgcgcc agcgcgtttc gccagttgga
     5341 tggcaatttg accgatcgcg ccaagcccta ccaccacgac aaaatcccct acgcgtacgt
     5401 tggcatcgcg cacgccgctc atggcaaact gcgccgggtc gtaacagacg gcgtttttcc
     5461 aggagctgcc ttgcggcatt ttgcgcagct tgtagttatt cactgcgtta atgatgaccg
     5521 tctcggagag cgggccgtag ccgcatacgc tgtcgcccac cgcgtagtct gtaacgtcgc
     5581 tgccgcactc gataatgtcg ccaaccacca tgttgccaag ctggaatttg ccaaactcaa
     5641 tgccgcgcgg cgcatctgcc ggacgcgggg tgaacatctg ccactcgccg ttaaagtctt
     5701 catcgataaa cgggctggcg gcgcggaagt cgaccacttc cgttccgtgt ttcggtgcgc
     5761 cgaaacgcac gcggatcttc acttcattgg ctaaaatcgc ccgatcttca tactcaacca
     5821 gcgcagcaac acgcggtgct gtggctacta actttttcat gtaaaactcc ttgtaaagaa
     5881 actggccgct ttagcccttc acgccaccgg cggtcaaacc actcttaata aaacgttccg
     5941 aaagcgcata cataatcacc accggcaatg cggtcaccag cgaggccgcc atcatccgtc
     6001 cccagatgta atcaggcgta ctgaacagcg cgttcaggcc cactggtaag gtgaaattgc
     6061 tggcgctgga gaggaaaatc gacgcaaaca gatagtcgtt ccacgtcacc ataaagcagt
     6121 agacgaacac cgatatcagc ccggacatcg ccaggggcac ggtgatacgg aagatgattt
     6181 gcagacggtt gaggccatcc atcatcgccg cttcttcgat ctcatcaggg atggtgtcga
     6241 agtaactttt cagcataaac acggcggtgg gcagggtttg cgtcaccatg gtaatgatca
     6301 gcgccatctc ggtgtcgtaa ataccaagcg cggtgatgat tttgaacaga ggcaccacca
     6361 gcaaaatgcc agagaacata tacaccgtgt aaaagctggc gttgatggtc atccggcctt
     6421 taaagcgcag gcgggaaagc gcgtaagctc cgagaatgcc gagaaatacc gccaccacgg
     6481 aggaaaccac cgacaccacc atgctgttac ggaagtagtc gacaaacggg aaaatcatcg
     6541 ggttaaaaat gtcgacgtaa tgctccagcg tccactgttg cggcagcagc gtaggatgca
     6601 gcgatatcgc ctctttcgcg cttttgaacg aggtcatcag catcacaaaa aacggaaaca
     6661 gcgtgatgat gagaaacagc gccagcccgc agtaaaaacc gatacgactg agcgtgcgtt
     6721 tatttgttgc cattgaggtt cacccgtttc ctggtcagca gaatgacagc gaaaataatg
     6781 atgaagagca ccaccgagat cgccgccgct ttccctaagt cattaaaggc aaaggcggtt
     6841 ttgtagagat agacaccaag aatatcgacc ttggttgtca gcagataaac atcagcgaac
     6901 atgtagaaca tccagatggt gcgcagggtc accaccgtcg ccaagaccgg cataattgcg
     6961 ggcagcgtga cgatgcgaaa ccgctgccag gcattagcgc catccatctc tgccgcttcg
     7021 tacagcgatt tgtcgatggt ctgcaaaatc gccagaaacg agataaaggc atacgggaag
     7081 tagcgccaga tggcgagcag caccaccagc acaaagctac tgcccggatt atcaaaccac
     7141 agcggtgcct gctcatagag atgcagaaga tcgacgccga ggtagttcac aatgccgtag
     7201 ccgttgttga acatgtattt ccaggcaaac accagcgaaa tggacggcgt tacgtaggag
     7261 aggatcacca gcgaacgcgc ggttttgcgc aggcggaact cgcggttgaa aaacatcgcc
     7321 acggccagcc cgagcacggt gctgcccgcc accaccagcg ccgtatacca gaccgtcatc
     7381 cacagcgagt gccagaagcc gggatcggag agaatgcgca catagttact caccccaaca
     7441 aacgttgact cgatgttggg attgagcggc agacgtaaaa aactgatttc gatattcgac
     7501 accatcggcc acgccaccag accgcccagc agtaataagc tgggcgcgag aagcagcagc
     7561 gcaaacggca tatcggaacg acctgaaaaa agcctgttca ttgcggattc ctgtcctggg
     7621 tttagtgctg ttcgatcagt tcatccagct ttttctggct cgcttgcagc gtgccaggga
     7681 gatcggcttt acccacggtg acgttatgca ccatggagct caccacacca gaacccgtca
     7741 catcgcccat gcgggtaaag tttttatccc ctactgcgcc aaagacctga atatttggca
     7801 gttcaccgat tagctgattc ggtagttcac ccagcgcctt aataacgtcg ttgtctttcc
     7861 aagtggcggt agtgacaacc gctttattca ccggcagcgc agcacctggc gacatcatca
     7921 cccagtcggc aatgttgtcc gcctgctcca taaaggtgac aaatttttct gctgcttcgg
     7981 tcttttgtcc ggcggtaatg gtcagcgagg tcaacatgcc gtagaccgca gagtttttct
     8041 cggttggcac cacgaaaccg acgtttttcg ggtcgccttc tttaatcaca gccggaagga
     8101 tataggtgga gtaaatcgcc atcggcgcgg tgccgttcat aaaggcgtct ttcacttcca
     8161 tgatgtcgtt agaacccggc atggtgttgg cagcaaggtc gcggtaatag gtcagcgcct
     8221 gcatcatctc tggtgtatca agggtgattt tgccttcggc gttaaagacg ttggcctagt
     8281 tggataacgc aaactgggag aaggattgtt ccgtcagcac gctttctgcc gtaggcagcg
     8341 caataccgta ttttttattc gccgggtcat tcagtttctg tgcaacgtcc agcagctgtt
     8401 gccagttttt cggctcctcc agccccgctt ttgccagcac atctttgcga taccagatgc
     8461 cgccaatcca ggcgctgaca ggaacaccgg tccatgcgct accatcttcg gtacgcacaa
     8521 tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt gctgatgacc gtggcaaccg
     8581 ctttgcgatc gataagctgc tctttatcca taactttggc gtagtcatgg ctggtttcga
     8641 tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac tttggtgtta taagcgtctt
     8701 cttccacggg cacttgttta acggtgatac ctgggttttc tttttcaaaa cgggcaatca
     8761 atttgctgat aactgcctga cgctcctgct ccaccgaaga atgcataaat tcgatggata
     8821 catgggtttt attttcttct ttacagcctg aaatcagggc gcaggaaacc agtgctgata
     8881 acagcacgat ttttgattta atcatgagat gttccttttt atttaatcca cataacctgc
     8941 caagggcgca gcgttatttc attacccaat atatcaactt cactaatcag atcccgacca
     9001 tgcagatcag taatatttac atgctgaata tttttactga cattaaacaa tccagtcaga
     9061 caattaccat cagcgttact tctttgaata cgcattacgg atgaattaac cgtatcgatg
     9121 gtaaaattat tatccggatg aaactcgtta tggctgcgac gaagtgtaat taaacgcgac
     9181 aactcatgat ataccgcatg ccttaatgta gcttcatcgt tcagttctcg tgttatctct
     9241 tcactgtgat attttttacg gttaatcgca cggttatatc cgagtttttc gacacctgcg
     9301 taatcattac gcgagccaag aatactttga atatatatcg cgggtacacc ggggaaactt
     9361 aacaaaatcg catgggcaag gataaacctg gcgcaacgtt cttcatcgct actctcgcgg
     9421 cggcttaacg catccatata ggtgacattc atctcatagg gactgcgcgt gccgtcggga
     9481 ttatttttcc agtttac
//
LOCUS       DADLTF010000145         9252 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.146, whole genome shotgun sequence.
ACCESSION   DADLTF010000145 DADLTF010000000
VERSION     DADLTF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.146"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..366)
                     /locus_tag="JN749_003388"
     CDS             complement(<1..366)
                     /locus_tag="JN749_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369680.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            890..1864
                     /gene="hemB"
                     /locus_tag="JN749_003389"
     CDS             890..1864
                     /gene="hemB"
                     /locus_tag="JN749_003389"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HAY7369681.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="JN749_003390"
                     /pseudo
     CDS             complement(1970..2821)
                     /gene="tauD"
                     /locus_tag="JN749_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="JN749_003391"
     CDS             complement(2806..3645)
                     /gene="tauC"
                     /locus_tag="JN749_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HAY7369682.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAVTRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQW
                     VNV"
     gene            complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="JN749_003392"
     CDS             complement(3642..4409)
                     /gene="tauB"
                     /locus_tag="JN749_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HAY7369683.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKCIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="JN749_003393"
     CDS             complement(4422..5348)
                     /gene="tauA"
                     /locus_tag="JN749_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7369684.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDDKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            complement(5615..6130)
                     /locus_tag="JN749_003394"
     CDS             complement(5615..6130)
                     /locus_tag="JN749_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369685.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQKTQSDLSQGDQLTEMLSFLTSEER
                     EALTNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEAS
                     ILSESVNAKAG"
     gene            6323..6574
                     /locus_tag="JN749_003395"
     CDS             6323..6574
                     /locus_tag="JN749_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7369686.1"
                     /translation="MRQREDSGRDWPSLMIQQALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            6576..8669
                     /locus_tag="JN749_003396"
     CDS             6576..8669
                     /locus_tag="JN749_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7369687.1"
                     /translation="MQSYVTVNDLLGVPGMPATAKGIRQALQRFSRALGDVSRRRAGT
                     KAIEYHIDCLPEVTQKALRERYVEQLVATENNVSEVKTVTRTARNPDAVQAVEAYRGS
                     PQLMEERLNALTENQRRVSEARAALAVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAINANAKRGTNRTISRDPLYQWVLKYNRSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQANGRPMSEAYEDFVTEWQRRYADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEIV
                     GILPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRETLRVLNRD
                     LRSAFNALQQDKPLNARQKSAMRELPSWSELIEAIREGVEWYNNRPHSELPMKPNGKH
                     YSPAEFRKKRLAEENTEIEWLSDLELRDMFCPMVERPVRRCEIKWLGNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFPVSADYYKQQQRIKGMHK
                     RAEEKIRDAVDEGIQVIEQKTTEPWLDNVYRPVGNVMTVQQQEYEEEHDEESERNFRR
                     GLQKLIAMQDDDPLA"
     gene            complement(8783..>9252)
                     /locus_tag="JN749_003397"
     CDS             complement(8783..>9252)
                     /locus_tag="JN749_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7369688.1"
                     /translation="QHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEHWGYVGAKSRQ
                     RWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKL
                     HVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2341 a         2393 c         2317 g         2201 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccccctc agggacgtaa taagttgatc catcaattat caatgcatag
      421 aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta cagacgaatg gttcaattat
      481 tctttcccct aagtccgaat gtggacatat ataatttatg gagaaaaaca cgcatcaaat
      541 tgataaaagg acgatatttc agcatgttgc agataatgtt atttttgatg catacgcgtt
      601 ataaatatca aaaacacaaa ccagataaat atcttatcaa tatacaacaa agtttgaatt
      661 attcaattta tagcagcaca accaaacgcc aaaaaaataa taaccaagag ctataaataa
      721 agattaaaaa tattttcagt gaatatgaaa ttattgctat ttctattaca aagatgtcct
      781 ggtctctctt tcattctctt cttgtcaaca tcgcgacaac tttcgtaaaa cactcctgcc
      841 ccgcttcagg tatactgtgc accttaattc cacaaacatc aggcagacca tgacagactt
      901 aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac
      961 aacacttagc cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta
     1021 caaagccgtt gaagccatgc caggcgtgat gcgcattcca gagaaacatc tggcacgcga
     1081 aattgaacgc atcgccaacg ccggtatccg ttccgtaatg acttttggca tctctcacca
     1141 taccgatgaa accggcagcg acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg
     1201 catctgcaag cagactgtgc cagaaatgat cgtcatgtca gacacttgtt tctgtgaata
     1261 cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct
     1321 ggaaaattta ggcaagcaag ccgtggttgc agctgctgca ggcgcagact tcatcgcccc
     1381 ttccgccgcg atggacggcc aggtacaggc gattcgtcag gcgctggacg ctgcaggctt
     1441 taaagatacg gcgattatgt cgtattcgac caagttcgcc tcttcctttt atggcccgtt
     1501 ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa agctatcaga tgaacccaat
     1561 gaaccgtcgc gaggcgattc gtgagtcact gctggatgaa gcccagggcg cagactgcct
     1621 gatggttaaa cctgctggag cgtacctcga catcgtgcgt gagctgcgtg aacgtactga
     1681 attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct
     1741 ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc ttaggttcaa ttaagcgtgc
     1801 gggtgcggat ctgattttca gctactttgc gctggatttg gctgagaaga agattctgcg
     1861 ttaatttttt tcctctcaga aagggggagt taacctgttg aaagtacggt gaacgccccc
     1921 tcaccctaac cctctcccca agggggcgag ggaaccgtcc acactcgtat caccccgccc
     1981 gataaaacgg tttatcccca aggatcgtcg cccgatgcat aatccgccgc tgtggcaggt
     2041 aatcggcatt ggcatagtgc tgagtcacgc ggttatccca aatcgcaata tcattcggtt
     2101 gccagcgcca acgcacctga aactccggtt tagtgatatg ggcgaataaa aagcctaaca
     2161 aggcttcgct ctctttctcg ctgacatcga caattcgcgt agtaaagcct tcattcacaa
     2221 acagcgcctg tttaccgctc accggatgcg ttcgcaccac cggatgtagc aacggaggat
     2281 ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc ggttttgcgg tatttgtatt
     2341 ccgggaatga tttacggaaa tcatgctccg cacgcagcca gctcagcagc tggcggaagg
     2401 gaacagagag cgcctcatag gccgcaatac cgctggttca gagcatatca ccgccggtcg
     2461 aaggtaactc tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat
     2521 cggtatgcca gttgtcgtta tccggcggat tatcgttatg ggtatccagc acgatgatct
     2581 cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg cagttcgcca aaacgctggg
     2641 ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg cagaaatacc acctgatggc
     2701 gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc gtcaggtcgg
     2761 cacccgaaat ttgtgcgccg atatgcggcc ccagcggggt aatgctcaga cgttcaccca
     2821 ttgtacttct ccatgccagg gcgtcagacg gcgctgtaac gcgcgcagac ccagttctaa
     2881 aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagt accacgtcag tcgcgagaaa
     2941 ttcacccgct gactgaacca taaatcctaa accgcgtgtc acggcaatca gctccgccgc
     3001 taccagcgta gaccagccca cacccagacc aatgcgtaat ccggtgagga tttccggcag
     3061 cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca gcgactgggc
     3121 ggcacgaatg cgaacctgct gcgcgctttt cacccctgcc agcgccgaca tcgccaccgg
     3181 cgcaaaaatc gctaaataga tcagtaagat cttcgaggtt tcaccaatac caaaccagat
     3241 caccatcagc ggcaaataag ccagcggtgg caccggacga taaagttcga ttaccggatc
     3301 cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa taccgatcac
     3361 caccgccgcg agtagcgcct gcacaatgcg cgtcaggctg gcagccagat gctgccacag
     3421 cgtggcgtcc ataaatccct gcggtccggc aatggtgagc agttttgcca gtacttgttg
     3481 cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc gccaccgtcc accatacagt
     3541 gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg gccagcgcca
     3601 tttcagccgc cgcgaatgca gtttttcatt aatgagcaca ctcatgagaa cgcctcccgt
     3661 tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg atcggacttg
     3721 atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg cagccgctcc
     3781 agcacacggc cagggccgga tgaaagcaga accagttcag tcgccataaa caccgcttct
     3841 tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag tttcagcagc
     3901 agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac caaacggttc atcgagtaat
     3961 aacagctggg gattcgccgc cagcgcgcgg gcaatcccca cccgctgacg ttgaccatcg
     4021 gaaagctgcc agatatagcg tttttctgcg ccttccagcc ccactttttt cagcatctgg
     4081 tgcgcgattt ccagtcgctg cattttctct atacctgcca attgcaggcc gaacgccacg
     4141 ttgtcctgta cattgcgcca cggtagcagc ccttcattct gaaaaactac gccacgctct
     4201 gctcccggtc cctcaataca cttacccgcc agttgaatgc tgccatgctg ataaggcaca
     4261 aaaccggcaa tcagattcag cagggtggtt ttaccgcagc cggacggccc cagcaccacc
     4321 agtagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt gccgccataa
     4381 tcggcataga gatgagagat ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag
     4441 gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc tttcaaaaac
     4501 tgcgcggtgt cgatgattgc tttgttcacc ggtccggtca gttctgccgt ttgttgctgc
     4561 ggcgtcagat aggtatttcc cttcaccagc cccggaacgt caccttcagg cacgccgctt
     4621 aaacgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtcagg gttagcaatg
     4681 tacggttgct gtgcatcgat ggcgcttttg gcgaacgctt tcacgacctc aggatgtttc
     4741 tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca ctgcccgacc
     4801 tgttcagaat cggtcagcac cttgtcgtct ttttccaggg cgttaaccgc cggtgcccag
     4861 acataagcac catcaatatc accccgctgc catgcggcga taatcgcggg cggctgcagg
     4921 ttcacaatct ccactagccc tggtttaatc ccccagtgtt tcagcgccgc cagcaggctg
     4981 tagtgggtgg tggagataaa cggtacggca atgcgtttac caatcaaatc ttcgggtttg
     5041 ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc cagcaagaag
     5101 acttcaatcg gcacctgttg gctggctgca accgctaatg ggctggatcc gaggttgccg
     5161 atttgcacgt cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc
     5221 cagtccacgc tcgccccgct ctctttagca aaggtgttgt cggcctgagc cactttcgcc
     5281 ggttcggctg aggtttgata cgccacggtg acgttcaccg cctgtgcctg aaaagcgatg
     5341 aatgccagtg cggcaagaag tgtgttatat gactaggtaa tttattcaag ataaccggga
     5401 atgggtaaaa aaagacggga tttgatggga ttcaataagt tatgcgccga ttgcattcaa
     5461 gataaaacga gttaaaaatg ctcaatgctt cgatatcgat aaaacattca agataggcgc
     5521 gccgtgtatt ttaattcttt taagactctt caaaaatgtc atcgtagcct ttaaaaacga
     5581 attttaaagg caccctaaag gcaggtcaat gaagttaacc cgccttcgcg tttacactct
     5641 ctgagagaat actagcttca atctcttcca aaatctctct gacttgctcg tcgggcatat
     5701 caacgagcat caacgctgcc aggcgtttac ggccctctag ttgcagtagc tcttgagttt
     5761 ccttatctaa caagcgtagc acagtctcaa cacctttcct tgcgagcatg tttgtaaggg
     5821 cttctcgttc ctcactggta agaaacgaca acatttctgt tagctgatcc ccttggctta
     5881 aatcactttg tgttttttgt gtttttggtg gattcccgtt accggttttt agccattcaa
     5941 gactaacacc ttcactttct gcaatgcgta ccaatatgct ttctcgaggt tctggtgtat
     6001 ttcccttttt aaaatagctt tgtaacgtat tgatatttat gccacaggct ctggcggcag
     6061 cacttcttga tggataccgc ctaataagat aagccatgcg ctccacgata ggttcttttg
     6121 cctgctccat aaaagtacct ctaggtattt ttctgtataa aggttctttt ccatctatct
     6181 aattgaatgg aaaggtaatt ttaaaaatcc aaataaaata caaaaaaata ccttgtattg
     6241 gtgctttttg tgatctgcga ttttatttac tcggatatcc acgcgaatat ccgcgcgggt
     6301 aattttgtag ggtaaaggaa agatgagaca aagagaagat agtggccgcg attggccttc
     6361 gctgatgatt cagcaagcac tcaaagataa tgggtggtct tcctttcggg agctttcact
     6421 ccatgccggt tatgctcagg attcactgaa agcggtagtt cgtacgccaa acaaaaaata
     6481 cgaacgtgtt atttctgacg ctataggtgt accgccagaa atcatctggc ccagccggta
     6541 tcgtaacgat ggatttatca ggaaggcgtc gtgatatgca gtcgtacgtt accgtaaatg
     6601 accttctcgg tgttcctgga atgccagcca ccgcgaaagg catccgacag gcgttacagc
     6661 gtttttctcg tgctcttggt gacgttagtc gccgtcgagc aggtacaaaa gccattgagt
     6721 accatatcga ttgcctgcct gaggtaaccc aaaaggcact gcgtgaacgc tatgtggaac
     6781 aactggtggc tacagagaac aacgtctccg aagtgaagac cgttaccagg acggcgcgta
     6841 accctgatgc tgttcaggca gttgaagcat atcggggttc tccgcaacta atggaagaac
     6901 gcctgaatgc cctgactgaa aatcagcgcc gggtgtctga agcaagagcg gcgctggcag
     6961 tggaagtgct gaagctggaa agcgccggta acctcgggcg actgaaagcc attaactttc
     7021 tggttgaaaa agcccgtaaa ggtgagttgc cggaacgact gcaacaggct gcgattaacg
     7081 ctaatgcaaa acgtggcact aatcgcacca tcagccgtga tccgctttac cagtgggtac
     7141 tgaaatacaa ccggtcgcag aacgccgcag aacgactgtt gttactggca ccaggaaaac
     7201 gcgacgaaat aaaaccggaa gaaattagct ggctgcctga atttctggcg cagtatcgtc
     7261 aggctaacgg cagacctatg tcggaagcct acgaggattt tgtcacggaa tggcaacgtc
     7321 gctatgcgga cgaaccgtac atgctggagg tgatgccttc ctatgatgtt gtccggtatg
     7381 ccatgaaaaa actgccggaa gtggtgaaac aaaaagggcg cgttaccggc agtgaatacc
     7441 gtcagcttga aggattcacc cgccgcgact ggacagctat gccggtgaat tatgtctgga
     7501 ttggtgacgg ccacggtatg aagctgaaat gtgcgcatcc gatccacggg cgtccatttt
     7561 caccggaagt cacgtttgtt atcgacggtg gaacccggtt tgttgttggc tggagtcttg
     7621 atcttgcgga gaacgtgttt gctgttgccg gggcgataca gcacggaatt cgcaatcacg
     7681 gaaaaccctt cctgtattac tcggataatg gttccggtga aacggcagat atgctggata
     7741 aagagatcgt cgggattctg ccccgtctgg gtattaacca ccccaccggt attgccggaa
     7801 atccgcaggg acgcggcatc attgaacgtc tgaaccggac gctgccgatg cgtatagcca
     7861 gacggtatcg cacctatttt ggcaaggggg cagaccggga aacactgcgt gtactcaatc
     7921 gcgatctgcg ttcagcattt aatgccctgc aacaggacaa accgctgaat gcccgccaga
     7981 aatctgcaat gcgcgagctg ccgtcctggt cagaactgat tgaggctatt cgtgaaggag
     8041 tggaatggta caacaaccgc ccgcattctg aactgccgat gaaaccaaac gggaagcatt
     8101 acagcccggc tgagttcaga aaaaaacgcc tggcggaaga aaacaccgaa attgaatggc
     8161 tgagtgatct cgaactgcgc gacatgttct gcccgatggt ggagcgtccg gtaagacgct
     8221 gtgaaatcaa atggcttggg aatatttatt acgcgccgga gctgcgtgat gaacatggtc
     8281 gcaaagtgct tatcagctat gacattcatg atgcagagcg aattacagtg cgccgtaagg
     8341 atggcagttt tatttgtgag gcgatatgga acggcaataa acgcgccgcc ttcccggtca
     8401 gcgccgacta ttacaaacaa cagcagcgta ttaaaggaat gcacaaacgc gcagaagaaa
     8461 aaatccgtga tgccgtggat gaaggtattc aggttattga acaaaaaacg acagaaccct
     8521 ggctggataa tgtttatcga cccgttggca atgtgatgac cgttcagcaa caggaatatg
     8581 aagaggagca cgacgaagaa tccgaacgca atttcaggcg gggattacag aaactcattg
     8641 ccatgcagga cgatgaccca ctggcctgaa taaaaaaaca gtccgcagca acggactgtt
     8701 aaacaggtaa ataaagctga tttgaaataa ccgaggaaac aatatgaccg atattaatga
     8761 tgttttcggt aatgactcca acttactgat agtgttttat gttcagataa tgcccgatga
     8821 ccttgtcatg cagctccacc gattttgaga acgacagtga cttccgtccc agccttgcca
     8881 gatgttgtct cagattcaga ttatgtcgct caatgcgctg agtgtaacgc ttgctgataa
     8941 cgtgcagctt tcccttcagg cgtgattcat acagcggcca gccatccgtc atccatacca
     9001 cgacctcaaa ggccgacagc aggctcagaa gacgctccag tgtggccaga gtgcgttcac
     9061 cgaagacgtg cgccacaacc gtcctccgta tcctgtcata cgcgtaaaac agccagcgct
     9121 gacgtgattt agcaccgacg tagccccaat gttcgtccat ttcagcgcag acaatcacat
     9181 cactgcccgg ttgtatgcgc gaggttaccg actgcggcct gagtttttta agtgacgtaa
     9241 aaccgtgttg ag
//
LOCUS       DADLTF010000146         9240 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.147, whole genome shotgun sequence.
ACCESSION   DADLTF010000146 DADLTF010000000
VERSION     DADLTF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.147"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="JN749_003398"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="JN749_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HAY7369689.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="JN749_003399"
     CDS             1828..3147
                     /locus_tag="JN749_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HAY7369690.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3512..4657
                     /locus_tag="JN749_003400"
     CDS             3512..4657
                     /locus_tag="JN749_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7369691.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4582..5199
                     /locus_tag="JN749_003401"
                     /pseudo
     CDS             4582..5199
                     /locus_tag="JN749_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5457..5789
                     /gene="ddpX"
                     /locus_tag="JN749_003402"
                     /pseudo
     CDS             5457..5789
                     /gene="ddpX"
                     /locus_tag="JN749_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5803..6699
                     /locus_tag="JN749_003403"
     CDS             5803..6699
                     /locus_tag="JN749_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HAY7369692.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6696..7682
                     /locus_tag="JN749_003404"
     CDS             6696..7682
                     /locus_tag="JN749_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7369693.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="JN749_003405"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="JN749_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JN749_003406"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="JN749_003406"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HAY7369694.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2255 a         2256 c         2321 g         2408 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acacttagtt aaacagctat aaatccctaa taaaaatgta tgaatatgaa
     3301 aaaccatacg atattcgctg tcaccgaaaa ataattacga tgggctatac tcttctgaag
     3361 aacagtagcg tagattgaaa ctgtcttaag tcgcttattt cttttcagta tatcttcata
     3421 tttcaggaga atatggtgca gttagtcgat ctcgcacgtt gcgtttcgtt ttgattccgt
     3481 ctgatttccc agccttataa gggtgtgaat tatggagatg tattttaaaa gaatgaaaga
     3541 tgagtggacc ggacttgtcg aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat
     3601 tgcccttgcg catgctcatt atctgagtat cgagttttat cgaattgtcc gcatcgaccc
     3661 gcatgccgaa gaattcttga gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga
     3721 acgctggatt attaacgtgc tttctgccca ggttgacgat gtcgaaaggc taatacaaat
     3781 ccagcatacc gtcgcggaag tgcatgcccg cataggaatt ccggtagaaa ttgtcgagat
     3841 ggggtttcgg gtgctgaaaa aaatcctcta tccggtaatc ttctcttcgg attattccgc
     3901 cgcagaaaaa cttcaggtct accatttctc gattaacagt attgatatcg cgatggaagt
     3961 gatgacccgc gcgtttacct ttagtgacag tagtgcctca aaggaagatg aaaactatcg
     4021 tatcttctcg ttactggaaa acgccgaaga agaaaaagaa cggcaaatag cctcaatact
     4081 ttcatgggaa atagatatta tctataaagt cctgctggat tctgatttag gcagcagttt
     4141 gcctttaagc caggctgatt ttggcctgtg gtttaaccat aaaggtcgac attattttag
     4201 cggcattgcc gaagtgggcc atatctcccg tctgattcag gatttcgacg gtattttcaa
     4261 tcaaaccatg cgtaacacca ggattttgaa taacagaagt ttgcgggtga aatttctatt
     4321 acagataaga aataccgtat cgcagattat taccttgctg cgtgaattgt ttgaagaagt
     4381 atcgcgccac gaagtcggta tggatgtact gacgaaatta cttaaccgcc gtttcctgcc
     4441 gactatcttc aaacgcgaaa ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct
     4501 gattattgac gttgataaat tcaaagagat caacgatacg tggggccata acactggcga
     4561 tgaaattctg cgtaaagtct cattcctctc gcagaagaga ttggtgaaat cgaaaatatt
     4621 gggcgctggg tcatcgcgga agcttgccgt cagttagcag aatggcgtag ccagaatatt
     4681 catatcccgg cgttatccgt gaacttgtcg gcgctgcact ttcgcagtaa tcagctgcct
     4741 aatcaggtgt ctgatgcaat gcaagcctgg ggtattgacg gccaccagct gacggtggaa
     4801 atcacggaaa gcatgatgat ggaacacgat accgaaatct ttaagcgcat tcagatcctg
     4861 cgtgatatgg gcgtgggttt atcggtagat gattttggca cgggcttttc cggattatcc
     4921 cgcttagtca gtcttccggt aacggaaatc aaaattgaca aaagctttgt cgatcgttgt
     4981 ctgacggaaa aacgcatcct ggccttactt gaagccatta ccagcattgg gcaaagcctc
     5041 aatttaaccg tcgtggcgga aggggtcgaa accaaagagc aatttgagat gctacgtaag
     5101 atccactgtc gcgttattca gggatatttc ttttcccgcc cccttcccgc cgaagaaatt
     5161 ccaggctgga tgagcagcgt gttaccgctg aaaatctgac aaattcctct cgcccgcact
     5221 cgcgggtttt cctttaaggt gacactgtca cttaaatact gtgatttcag ctacgattcc
     5281 gggagattcc ttccttaact ttccctcttg caccaaaact gcactacgct gcacaaatgt
     5341 tgatcaaaag ttaaaaaata taactttaag ccagcattct ggcgcttatc ccggcatggc
     5401 atgagatctg cataagcgga aagcgcagca atttttgtct tatacaggaa ccgtttatgt
     5461 cggataccac cgaactggtt gatttagccg tgatcttccc tgatctggag atcgaattga
     5521 aatatgcctg cgttgataac atcacaggta aagctattta tcagcaagcg cgttgtctgc
     5581 tacataaaga tgcgattacc gcgctggcga aaagtatcag tatcgcccag ctgtcaggct
     5641 tacaactggt gatttacgat gcgtatcgcc cacaacaagc acaggcgatg ttgtggcaag
     5701 cctgcccaga cccgcaatat gttgttgatg tgacggtcgg ttctaatcac agccgtggca
     5761 cggcgctcga cctgacgctt cgtgatgacg tggaggtggt gaatgatgct aagcgaggaa
     5821 acgtccgccg tacgcccaca aaaacaaacg cgatttaacg gtgcaaaact ggtatggatg
     5881 ctgaaaggca gtccgctcac cgtgaccggc gcagtcatca ttgtattaat gcgactgatg
     5941 atgatttttt caccgtggct ggcgacgcat gatcccaacg ccattgattt aaccgcccgc
     6001 cttttgccgc cttctgcggc gcactggttt ggcaccgatg aagtgggacg cgatctgttt
     6061 agccgcgtac tggtcggcag tcagcaatca attctcgccg gattagtggt ggtcgccatt
     6121 gcgggtatga ttggttcgct actcggatgt ctatccggtg tgcttggcgg acgcgcagac
     6181 gccatcatca tgcgcatcat ggacattatg ctgtcgattc cttcgttggt actgacaatg
     6241 gcactggctg ccgctctcgg gccgagttta tttaacgcca tgctggcgat tgctattgtg
     6301 cgaattccct tttatgtgcg cctggcgcgg gggcaagcat tagttgtacg ccagtatacc
     6361 tatgttcagg cggcgaaaac ctttggcgcg tctcgttggc atctgatcaa ctggcatatt
     6421 ttacgtaact ccctaccgcc gctgatcgtg caggcatcgc tggatatcgg tagcgcgatt
     6481 ttaatggccg ccacgttggg atttattggc ctgggtgctc aacaaccgag tgctgaatgg
     6541 ggcgcgatgg tagcgaatgg tcgcaactat gtactcgatc agtggtggta ttgcgcattt
     6601 ccgggggcag cgattttgct taccgccgtc gggtttaatc tctttggtga tggtattcgc
     6661 gatctgcttg acccgaaagc aggaggaaag cagtcatgac ccaacccgtt ctggacattc
     6721 aacagctgca tttgagtttc cccggtttta acggtgatgt tcacgcgctc aacaatgtgt
     6781 ccttgcagat taaccgcggc gaaattgtcg gtctggtggg agaatccggc tcaggtaaat
     6841 cagtcaccgc aatgctgatt atgcgtctgc taccgacggg cagttattgc gtacatcggg
     6901 ggcagatttc actgctggga gaagatgttc ttaacgctcg ggaaaaacag cttcgtcagt
     6961 ggcgcggcgc acgagtggcg atgatctttc aggaaccgat gaccgccctt aatccgacac
     7021 gtcgaatagg tcttcagatg atggacgtaa tccgccatca tcaaccaata agtcgtcggg
     7081 aagccagagc taaagcgatt gccctgctgg aagagatgca aatcccggat gccgtggaag
     7141 ttatgtcgcg ctatccgttt gagctttcag gtggtatgcg ccagcgggta atgattgcgc
     7201 tggcattctc ctgcgagccg caattgatta ttgccgacga accgacgacg gcgctggacg
     7261 tcacagtaca gttgcaggta ctgcgtttgc ttaaacataa agcacgcgcc agtggaactg
     7321 cggtactgtt catcagccat gatatggccg tggtgtcgca actgtgcgat agcgtttacg
     7381 tgatgtatgc cggaagcgta attgaaagcg gcgggacggc agacgttatc catcatcccc
     7441 ggcatccgta taccattggt ttgctgcaat gcgcaccaga acatggagta ccacgccagc
     7501 cattacccgc cattccaggg acggtaccaa acctcaccca tttgcctgac ggctgcgctt
     7561 ttcgcgatcg ttgctatgcg gcaggtgcac agtgtgaaaa cgtcccggcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcgcctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggtaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttactgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gagctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgagctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
//
LOCUS       DADLTF010000147         9128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.148, whole genome shotgun sequence.
ACCESSION   DADLTF010000147 DADLTF010000000
VERSION     DADLTF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.148"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..171
                     /locus_tag="JN749_003407"
     CDS             <1..171
                     /locus_tag="JN749_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7369695.1"
                     /translation="SVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNL
                     SQSKNKESTNDV"
     gene            521..883
                     /locus_tag="JN749_003408"
     CDS             521..883
                     /locus_tag="JN749_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7369696.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            880..1797
                     /locus_tag="JN749_003409"
     CDS             880..1797
                     /locus_tag="JN749_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAY7369697.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            1810..3075
                     /locus_tag="JN749_003410"
     CDS             1810..3075
                     /locus_tag="JN749_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HAY7369698.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(3142..3765)
                     /locus_tag="JN749_003411"
     CDS             complement(3142..3765)
                     /locus_tag="JN749_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7369699.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSERY
                     THTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGNNCELIDIGCTFMEIT
                     SKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVTFSHPLAAATNGYVVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(4507..4854)
                     /locus_tag="JN749_003412"
                     /pseudo
     CDS             complement(4507..4854)
                     /locus_tag="JN749_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
     gene            4976..5236
                     /locus_tag="JN749_003413"
     CDS             4976..5236
                     /locus_tag="JN749_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HAY7369700.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(5254..7989)
                     /locus_tag="JN749_003414"
                     /pseudo
     CDS             complement(5254..7989)
                     /locus_tag="JN749_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
     gene            complement(7990..>9128)
                     /locus_tag="JN749_003415"
     CDS             complement(7990..>9128)
                     /locus_tag="JN749_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7369701.1"
                     /translation="QEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQIESVRKG
                     MGFIDEDQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPQDVAQMYQQNP
                     RQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQ
                     ANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQLQQKQQAL
                     NDGYKDGINTLTTSMLTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQ
                     AYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAV
                     GKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
BASE COUNT         2378 a         2096 c         1954 g         2700 t
ORIGIN      
        1 tcagtgttac aggaaatggg aggctgggag tctatcgaaa tggttcgtcg atatgctcac
       61 cttgcaccta atcaccttac cgaacacgca cggcaaatag actcgatcct gaacccatcg
      121 gtcccaaatt tgtcccagtc aaaaaataag gaaagtacta atgatgtgta acttattgat
      181 ttaaatggtg ccgataatag gagtcgaacc tacgaccttc gcattacgaa tctgtagcac
      241 caatcataac tatctgtttt agcaagcatt aaccgcattc actaagatat agttgatggc
      301 acaaacagaa agttgatgaa tgatgttgcc atatatatgt cacaaatacg gcacaacgat
      361 cttcaaacat atagccacac attcacagaa gagcacaaag ccttgcaatc cagtgcaaag
      421 atttgtgtgc cagagttttt ctcatcaact accgcaagta tcgatcgatt gagacttgga
      481 tgatagactt catgcctttg attattagct gatagaagaa atgttaaagc tatttgcaaa
      541 gtacacatct ataggcgtgc tgaacacact tatacactgg gtggtttttg gtgtatgtat
      601 ctatgccgcg cataccaatc aggctatggc aaacttcgca ggtttcgttg tggctgtgag
      661 ttttagcttc tttgcgaatg caaaattcac attcaaagca tcaactacaa cgatgcgcta
      721 catgttatat gtcgggttca tgggaacact gagtgctact gttggatggg ttgctgatag
      781 atgttcactt cctccaattg tcactcttgt taccttctcc gccatcagct tggtgtgcgg
      841 tttcgtctat tcaaagttca ttgtctttag ggatgcgaaa tgaagatatc tcttgtcgtt
      901 cctgtcttca atgaagaaga agcgatacca attttttata aaacggtacg tgaattcgaa
      961 gagttgaagc catatgaagt agaaattgtt ttcataaatg acggaagcaa agacgctacg
     1021 gagtcaatca ttaacgctct ggctgtttct gatccgctag ttgttccgct gtcatttaca
     1081 cgcaactttg gtaaagaacc agcattgttt gcagggttag accatacaac cggcgatgcc
     1141 gtgattccaa ttgatgttga cctgcaagac ccgattgagg ttattcctca tcttattgaa
     1201 aagtggcagg caggtgctga catggtgctt gctaaacgtt cagaccgctc aactgatgga
     1261 cgactgaaac gtaagacagc tgagtggttc tataaattac acaacaaaat aagcacccca
     1321 aaaatagagg aaaatgtcgg agattttcga ctcatgtctc gtgaggttgt ggagaacatt
     1381 aaactattgc ctgagcgcaa tcttttcatg aaaggcatac tgagctgggt tggtggtcag
     1441 acagatgtcg ttgaatatgt acgcacagag cgtgttgctg gcatctcaaa atttaatggc
     1501 tggaaattat ggaatctggc actggaaggt atcacaagtt tttcaacctt tcctcttcgc
     1561 gtatggactt atataggctt gtttgttgca agcatttcat ttttatatgg tgcatggatg
     1621 attatagaca ccattgtctt tggtaaccca gtacgcgggt atccctccat gcttgtatca
     1681 atacttttcc tgggtggagt gcaactgatc gggattggtg ttctcggtga atatataggt
     1741 agaatttatt tagaaacaaa atcaagacca cggtacttaa ttaaaagcag aaaataacag
     1801 gatgatctta tgaagatatt cataatgcgc aattggcaca agatatcaat ttttatactt
     1861 gcatttactt tgatttggct taggcgcatt gacattttga caaatgccca gttttgggct
     1921 gaggatgcag ttttttggta taaagatgca tacgaacaag gttttttcag ttcactcata
     1981 accccaagaa atggttattt tcaaacagta tcaacactca ttgttggtgc gacgacattt
     2041 attaatccaa tttacgcacc attattatca aacttttttg gaataataat tagagctata
     2101 attatatggt tcttatttac cgatagattt aaattcctaa gcaccacatc aaaaatcttc
     2161 atctctgtat atttaatttg catgcccggg cttgatgaag tccaagccaa tataacaaat
     2221 gctcactggt atttatcatt gtatgttgca atgataataa tatctgaaga atccaagtca
     2281 aaattatgga aagcacatga tttatttttt atagtattat ctggattgag cggtccattt
     2341 attatattca taattgcatc atctgttttt aaatatttac acttatcaaa aggagtaata
     2401 agcataagag gtttatactt attttatact cgactgccat atttggcaat gatcttgtgt
     2461 gggctgatac aggcaacatc aataattctt acatttaatg gaacaagatc acatgctcca
     2521 ttaggattca gttttgatgt tatgtcatct ataatatcat ctaatgtatt tcttttttca
     2581 tttgtccctt ggaatatcgc taatgctgga tgggataatc accttttatc gtacacacta
     2641 tcaactttag taattacatt atgtgttttc atttatatta aaggaaattg gcaaatgaaa
     2701 gtgtttgcca cccttccaat acttattgtc gcattttcta tggcaaaacc tcaactggca
     2761 gattctgttc ctcaactgcc aacacttgca accggaaacg ggtcaaggta ttttgtaaat
     2821 attcatattg caattttttc attaatatgc gtttgtttgt ttcaatgcat aaagaataaa
     2881 accttaaaaa tattctttaa aatctatgtt tcagtcatcc ttattgcaat gataaaatta
     2941 aactttttta taacccctct tcctgacatg aactggtctc aaggagcaga attaataaat
     3001 aaagcaaaac atggagaagc cgtctcattt acagtgctcc ctccatggtt aactcttgat
     3061 ttaataaaaa aataaaactt cacattaata aataacaatg aatccgcctc cctacgtatt
     3121 cccgtaggga ggctgtcgtg tttatagtag cctggtaatg ctaaatgcaa acgaactgga
     3181 gattcctgct ccgctaagcg tattgcgcag ggtcacccta aatgtatcta caccaatata
     3241 agtaacatca caactaactg aatcaggcgc agatataggt gttatctgaa ccacataacc
     3301 attagtagct gctgcgagcg gatgagaaaa cgttacatca tacacaccag tagcatgcgc
     3361 gttaacggat gaaacacccg gcgcaatgtc tacactacca ttggcgttaa ttctccctga
     3421 gcgcgtaact gactctgcag aaaccgatgt ggccttactg gttatttcca taaaggtgca
     3481 accaatatcg atcaattcgc aattgttccc tgttatccag tatggtcgat ctgcacttgg
     3541 ctgataatcc cctgcgctct ggaacgttgt gccattgagt aagactataa cttttccccc
     3601 acctgagctt gttacttgta tattcgtgtg cgtgtagcgt tcactgctca cgcgatgaaa
     3661 atttccaccg tttataacta ccgttacagg tcgcgtccca gtattatcta tagcaaggtc
     3721 tgcccccccg gcattagcct cgaagtatgg gttgtttaac accaaagcac acgaaccgtt
     3781 aaggccattt atggctaatt gcgcaccacc tgtaccggaa tccccctgag taccatttcc
     3841 ttcacaagtc aggctgttga tagtagttcc tgccccccat ctatcaccaa gtatacccca
     3901 tcgtgtattc tggcttgcag ttaatcggtt aactgtaatg gcattaagct ctgacccttc
     3961 actggagtta agtgcgcgcc agccaatacg gttaccctgt aagtacacat tatctaactc
     4021 tgacgttaac accgagtctg tacgcaaccc ttcgccggta gtgtgcccat aagacacaat
     4081 gttctctaat ttagtgtatg ctttattggt tacgagaaga ccgtaagacg atcccgaacc
     4141 tttaagtgta atgtccccca gataatcata ggcgtgtaca cgttgggttg ccccgactgt
     4201 atcaccgagc atctttaagt tgaaatccga ggcatggcag ttaaatatcg tttctgccat
     4261 attttctccg gaataatgag cacggactcc tgtcgaacca ataaatttag cgtcatagtt
     4321 gccgtaatca agaacgagtg gtgacgcgat atcgtacacc ccttgcttta tttttaatgt
     4381 tccaccgaaa ggccacgatg taagcgggtc ttgatatatt tcatttttta ttgcaaatag
     4441 ctcctcttgg agatttttgc cgctattagt gccaatgtga gcagctcctt catctgattg
     4501 aagtaactgc tttaactgat caggatcgta cttcagcaca ttctgaaaat agaactgctg
     4561 cgaaccatac gcatcatata cagccattga atggccttgc tcagttacaa atttggcaat
     4621 ctgcccgttg tacacaggat atccggcagc attgataaca attggctgag aaacagggac
     4681 gtgagatccg tcttcattct ctacataaac ctgaatctgg ttttcaggat ttaccgggtc
     4741 agtgtcaatt tttccgatat aaattttgcc attggctacg gctttaaaag aacgcgccat
     4801 agtgaagagt tgcgaaggca tgctgaccac aacatttgcg gcgatatctg acatttcatt
     4861 gctccagacg aatgatatga tgcaatcatg atgtgattgc ataccgaaat ggcactattg
     4921 aatatttatc cagtaggtta cgatgccatt ccacccaact ggtgaggcat caaggatgta
     4981 cagcaaatac gacgaggcac agtttcactt gagacttccg catgaactcc acgcgaaaat
     5041 taaacagtgt gcgaagatga ataacagttc gctgaactca gagataattg cagcgattga
     5101 agaatcattg gctaaacaaa gctctgcatc agtttacatt gacgatgcag agcgtatggc
     5161 agaacaacaa tctgatatgg ttaagaaaat tgtctttgat acgctcaagg agctatataa
     5221 aaaagacagc aactaaccat ccgtggcttt ccgttacttc tggttatcat cactgttcat
     5281 ccagtacatg atgcctagcc ttgatatggt tttcttatct gattctggaa gtgagttgta
     5341 aaacgagcgc caagcagaag agcgtcttac agttgtatca acgatgcttt ctggtgcttg
     5401 ttttactgcc agccttgctg ctttctggaa ttctggtgag ctaattagct tttctgcaga
     5461 ttcaattgat tcagcgccgc ccatcgccct gattttgctg gcgacttttg ccccaatttc
     5521 tgacccagca acagcaccta ctgcactgaa cggtcctgac acagttgaac caaccgcagt
     5581 gccaatgcgt tgggcatggt tagcaacaaa ttcatttgcc gcagtgacgc gattaaaccg
     5641 tttgacgaac tcgtttagtc ttcctgtagt tatctcgtaa gattttgcat ctttgatagc
     5701 ctttgcgacc ttatatacat cgttcagacc tgacatagtc tcttttggta aatgtctggc
     5761 aagattgcgc atctgcccgt ttgctaacat gttttgatac cagtcagcaa accctgcagg
     5821 attaaaatca gcgccacgtt ttccgttcga aagcatatcc cgaagacctg ttcccagtag
     5881 ctcggttctt agcttcctgg acggcgtgtt ttgcatcaat tcacgaaatc ctttggcatc
     5941 gcctttcgac attgcctgta gtgctgtagt tgctttgcga gaaacatcac cgttcagcgt
     6001 tctgccagtt agattaatca tttgctcttc catgcttttt cgcatctgaa caagcctctg
     6061 agcaacttca aaatcacgta atgcatttgt ctctgacagc gccgcctttt gatcatcggc
     6121 gagcgaaccg taaagcctcg ataaagcagc gcgatcagca tctttatatg gtccagaatt
     6181 cttatgaagt gcatcaccaa caagtcgtcg ttgcttattg agatttgcat aagttaatgc
     6241 gccgttcttg cctggattaa ctgcccgaaa gactctcttt tcaatagggt caaggttttt
     6301 catcccgcca agatcttctg ccacctgttt gagcattgac cttgttgatg gcgcatcaat
     6361 ttttgcacct gcaggcatcg ccttttcaac attgcgataa agctggtcgc ttcgtttcat
     6421 cagcgcagac attcttccat tgaccgtatc aataaacttg tcgctcatac caagagcatc
     6481 ttttgcaccg gaaacgcgat caattatctc acccgcgccc tgtgctagct gcctgattgc
     6541 ttcattttcc tgcacctgta gagcagaacc cgcacgggac ttgattgctt gctcaactgc
     6601 cttgtattgc tcgttcccgg aaaagtgtga agggagtaat gaatcaacat taagcctttc
     6661 agcagactcc agaacttcag cctgaggatt gatatctagt tcatcaagcg atgaagcgag
     6721 atttggcctt ttttgtgctg ctactgtgcg aacgacttcc tctgggttct tcgtcgcagg
     6781 cgtcattggt acaccttgtg ctactcttgc ctctgcggca tgaaccgcag gatttgttgc
     6841 tgcagcggca tcaccggaga atggtgatgt ttctgttggc gcagtggtcg ctctctgtgc
     6901 agatgtttta ttgccactaa ttatatttga tgcaccacga acaccacggg taacaccatt
     6961 tatcaggcca tgcgtagcaa tgctaagtcc ggcattaata gcggcatttt cggcaaaatc
     7021 accccgttga ttcgccgcgt cagcgagaga accaatgacc atgtttccag ctacacctgc
     7081 tccaggaaca agataaccgc ctattgactc tccagcttgc gcgtaggggt ctgtcggtct
     7141 gtctactgga cgataaacat catccaatac ttttggcccc ccaagcccct gactgattgc
     7201 attaatcaga cttgcgccac cctgcaatac gtcaaatggt atgtttacca gaccacgacc
     7261 agcctgttct gcaatttacc ctgcactttg accaccagtg agccaatcgc cagcttgttg
     7321 catcaatgat ggttcttccc gtgttggtgc attattggcc tgattaactg tttgttgctg
     7381 aacaggtttc ggtgcgacag aacttacggg ttgaggtggc tgctgactgg ctgcctgctg
     7441 ctcaatctga gcaaatggat tatttgggtc tgactgcaca cctgatgccg atacttgttc
     7501 ggatgactgt acttccagtt gtgcaaacgg gttgttaggg tcttgctgag gatgtacctt
     7561 tgcagaggtg gcgcgctgtt cgactgttga gtctgtcacc gggtcaccag cccattgagc
     7621 aaagcgatca tcaacgtaac cgcggccttc aggtcctggc gtatattcac cacgctttgc
     7681 cttcataacg ttgccgggac cgtcgtgata agcctgaaga gcgtcacgcc agttaccaaa
     7741 ctgctggtat atctttgcca gatagcgcgc gccagcgtca gcctgatatt cggggttttg
     7801 catttgctca tcggtataac ccatatcacg ccatgtccca ggcatgacct gagtcaatcc
     7861 tacagccccg gcggagctta ctgcggcagg gttgtaagaa gactccttgg cacccagtgc
     7921 agtcatcaac ccttctggca caccgtaacg tgcgccagcc tgctctaaca aatcacggta
     7981 attagccatt tactgcccca aagatggaag atatccgtag cgattaatga agtcgattga
     8041 cagctcgggg tgctgcttca ggtaatctat agaagcctga ggcgcttcca ctcgcttgat
     8101 accgttttgc tgaacgtact taccaaccgc ctcattacgc ttctggttga gcgtgttcag
     8161 gatgacgcca gcgttgcgac gaaaggactc ctcgctctgc gagttctgca gcgaaccaac
     8221 agcctggtcg agctttttgc cctcggcatc agaaagtgcg cccatgcctc gcatggcctg
     8281 aaccgctgtc aggtatgctt gggatttaaa ggtatcaagt cgtgcctgag tgtctgcagc
     8341 ctgtgagcct ggaacgttgg ggattactcc acgtaagcct gtaatgctct taagtgaagg
     8401 agaactaacg atatcgttca gagttaacat gctggttgtg agggtgttga tgccgtcttt
     8461 gtagccatca tttagcgctt gctgcttctg ctgcaactgc tggttgttgg ctgctatgcg
     8521 gctctgtatt tcctggcgct tcaggtcgtt agtttctgct gatagcatcc ggtcaaggcg
     8581 cttattttcg ttgttaatgc ggtttgtttc tgcgtccaga ttaatgcgct gctgacctaa
     8641 attcgcctgg atatcttgcc cgcgcattgt gattgcctga ttccgagcgg cggtttgcga
     8701 atccaggtcc tgaccgcgca tggtaacctg gcgaccctgc attttatcct gtaggtcaaa
     8761 gtatttttcg ggtccgagac tgttcatccc caggtgatcg acaaattcgc cgaactgccg
     8821 cgggttctgt tggtacatct gagcgacgtc ctgaggatta acgccaacac gagctaactc
     8881 accggcgttg ttttgcagcc atgattgcat tgcttctgga gacgatgacg caaggcgtgc
     8941 gccagccgct aaggtgccga tagaattacg ctggtcttca tcaatgaatc ccatgccttt
     9001 acgaacggat tcaatctggt ctggatattg agtagccaac tgacgcaaag caccgcgatc
     9061 accagacgca taagcattag cgtacgcctg ctgaaattct ttctgccgct gagcctgctt
     9121 ttcctgct
//
LOCUS       DADLTF010000148         9010 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.150, whole genome shotgun sequence.
ACCESSION   DADLTF010000148 DADLTF010000000
VERSION     DADLTF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.150"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(95..1471)
                     /gene="trkA"
                     /locus_tag="JN749_003416"
     CDS             complement(95..1471)
                     /gene="trkA"
                     /locus_tag="JN749_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HAY7369702.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="JN749_003417"
     CDS             complement(1493..2782)
                     /gene="rsmB"
                     /locus_tag="JN749_003417"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAY7369703.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="JN749_003418"
     CDS             complement(2828..3775)
                     /gene="fmt"
                     /locus_tag="JN749_003418"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAY7369704.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3790..4299)
                     /gene="def"
                     /locus_tag="JN749_003419"
     CDS             complement(3790..4299)
                     /gene="def"
                     /locus_tag="JN749_003419"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAY7369705.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4447..5571
                     /gene="dprA"
                     /locus_tag="JN749_003420"
     CDS             4447..5571
                     /gene="dprA"
                     /locus_tag="JN749_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAY7369706.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5543..6016
                     /locus_tag="JN749_003421"
     CDS             5543..6016
                     /locus_tag="JN749_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HAY7369707.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6045..6587
                     /locus_tag="JN749_003422"
     CDS             6045..6587
                     /locus_tag="JN749_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HAY7369708.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6592..7164
                     /gene="tsaC"
                     /locus_tag="JN749_003423"
     CDS             6592..7164
                     /gene="tsaC"
                     /locus_tag="JN749_003423"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HAY7369709.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7169..7987
                     /gene="aroE"
                     /locus_tag="JN749_003424"
     CDS             7169..7987
                     /gene="aroE"
                     /locus_tag="JN749_003424"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAY7369710.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7984..8241
                     /locus_tag="JN749_003425"
     CDS             7984..8241
                     /locus_tag="JN749_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HAY7369711.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8217..8771)
                     /locus_tag="JN749_003426"
     CDS             complement(8217..8771)
                     /locus_tag="JN749_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAY7369712.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2255 a         2189 c         2248 g         2318 t
ORIGIN      
        1 aacaaatgtt aagccatttt cctgccagga atgaatttaa ttaatccttg ataatcaaag
       61 ggaaataata aggcgtcatt agacgcctta ttaattacaa gaagaaaggg cttggctgga
      121 agagtctttc gacgtcggta ataaactttt tatctgtgag gaacataatt acgtgatcgc
      181 cttgctcaat gcgcagattg tcattggcaa tcatcacgtc gtttccacgt accaccgctc
      241 caataatcgt tcctggcggt agcttgattt cgtcaatgac tctgccgaca acgcgtgagg
      301 tgctttcatc accgtgagca acggcttcaa tagcttctgc tacgccgcgg cgcaatgagg
      361 aaacaccaac aatatctgct tttcgcacat ggctaagcaa cgcagaaata gttgcttgtt
      421 gtggtgaaat tgcaatatcg atcacgctcc cctgaaccag atccacataa gcgcgacgct
      481 ggatcaatac catcaccttt ttcgcaccca tacgtttggc aagcatggcg gacatgatat
      541 tggcctcgtc atcgttggtg acggcaataa acagatcaac ttgatcgata tgttcttcgg
      601 ccagcagttc ttgatccgac gcatcaccaa aaaagacgat cgtattctgc aacttttccg
      661 ccagttcggc agcgcgctgc tgattacgtt cgatgagttt aacgctgtaa tctttttcca
      721 gacgacgcgc cagccctgca ccgatattac cgccaccaac cagcatgatc cgtttatacg
      781 gtttttccag tcgctgtaat tcactcatca ccgcgcggat atgctgtgaa gcggcaataa
      841 agaacacttc atcaccagct tcaacaatgg tcgaaccttg tggacgaatg gggcgatcgt
      901 ggcggaaaat tgctgccaca cgagtatcga tatgtggcat atgttcgcgc atggtcgaaa
      961 gtgcattacc aatcagcggg ccgccataat aggctttaac tacagcgagg ctgactttac
     1021 cctcagcgaa gttcaccacc tgcaatgcgc ctggatactc aatcagtcgg taaatattat
     1081 cgataaccaa ctgctctggt gcgatcagat gatcaatcgg tacagcatct gaatgaaata
     1141 gcttatcggc atcgcgtacg tagtctggtg agcggatgcg agcgatgcga ttaggggtgt
     1201 tgaaaagtga gtaggctacc tggcaggcaa ccatattggt ttcatctgaa ctggttacag
     1261 caaccagcat atcggcgtcg tcggcacctg cctcccgcaa tacgcgtgga tgagagccat
     1321 gcccctgcac gactcgcagg tcaaatttat cctgcaaggt ccgcagacgc tcaccgttgg
     1381 tatcgacaac agtaatatcg ttgttctcgc caaccaggtt ttccgccagt gtgccgccaa
     1441 cctggccggc acccagaatg ataattttca tcagtcgcga cccgttatct catcactttt
     1501 tgattagctt agcgtaaaag aagccgtcgc cctcttcggc accaggctga ttttgtttac
     1561 ccggttgctc tggtgttcct gtttcgcaaa gttcggcatc agcggtacgt tgcaaaaagg
     1621 ctttaatctg caggctattc tcttccggta acaccgaaca ggtggcatag accagggttc
     1681 caccggattt taaatgcggc caaatggcgt cgagaatttc agactgcaat tgcgcgagtt
     1741 ccgggatatc gcgatcgcga cgtaaccatt taatatctgg atggcgacga atcacccccg
     1801 ttgctgaaca aggcgcatct aataaaatgc gatcaaactg ttgctcgcca caccattggg
     1861 aagggtaacg gccatcacct tgtttcacgg ttgccttcat accaaggcgt tttaaattgt
     1921 cgtaaacgcg agagaggcgc tgttcgtcaa tatcaaccgc aacaacctgc gcttctggtg
     1981 ccacctcaag gatatgcgtt gttttaccgc cgggggcggc acaaagatcc aagatgtgtt
     2041 caccgttttg tggcgcaagc caggtcatgc aaccttgtgc tgatgcatcc tgaacggtaa
     2101 cccatccgtc ttcaaaacca ggtagcgcat gaacaggtgc aggtgtttcc agccgtacag
     2161 catcagggta atccgcatgc gggaaacctt tcattcctgc ttcatccagc aatgcaagcc
     2221 agctgtcgcg ggaatgatgc gtacgattaa tacgcagcca cattggcgga cgctggttat
     2281 tggcttcggc gatggattgc cactgctctg gatacgcttt ttgcagacgc ttcagcaacc
     2341 aggaaggatg cagataacgt gcatcacggg tattaaactc ggctaataac tcttcttgcc
     2401 gacgctggaa ctggcgtaat acaccgttaa tcaacccttt aagttgcgga cgcttaattg
     2461 cgatagcgcc ttcaaccgtt tcagccagcg cagcatgagg tggaatgcgg gtataaagca
     2521 gttgatacaa accaaccata atcaggtaat gcacagtgcg ctgtttgccg gtcatcggac
     2581 gggccattaa cttattaatc agccagtcta actgcgaaag cgtacgcagt acgccaaagc
     2641 acaactcttg aagaagtgct ttgtctttat cggaaacttt ttgctggagc ggtggcagaa
     2701 tgttgcttaa tgattgccct tgctcgacga cttgttcaac ggcctgggcc gccatgctac
     2761 gtaaattacg ttgttttttc ataagtataa aaatgctcgg caagaccggg cttagaagag
     2821 tggactatca gaccagacgg ttgcccggaa caaaccattc ccgacgagag ttcaggaggt
     2881 cttgcgcgct catcgctttc ttacccgcag gttgtaacga gagcaggttc aggatgccat
     2941 caccagtcgc aacctgaatg ccttgtttgt tggcttcaag gatcgttcct ggtgcagcgt
     3001 tggttgccgt atcaatgacc gacgctttcc agactttaac cggctgtcct tcaatttcca
     3061 gccagctcat tggccatggg ttgaaagcgc gaatgcagcg ttcaagctgt gctgccgaaa
     3121 gtgaccagtc aatacgcgct tcttctttac tcaacttctc ggcgtaagtg acaagagttt
     3181 cgtcctgaac ttctggtttc gccgtgccgt ctgccaattg tttcaacgtg gtgataagcc
     3241 cttgtgggcc aagctctgcc agcttgtcgt acagcgtacc actggtatct tctgcagtaa
     3301 tcgggcagga gagcttatag agcatgtcac cggtgtctaa accaacatcc atttgcataa
     3361 tggttacacc agtttctgca tcacccgccc atagtgagcg ttggattggt gcagcaccgc
     3421 gccagcgtgg cagcagtgaa ccatgaacgt tgatacagcc aagacgcggc atctccagca
     3481 cagctttagg cagaattaaa ccataggcga cgacgaccat aacatcagcc tgcaaatcgg
     3541 cgaccagttg ctggttttct tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt
     3601 cttcctcagc cagaacttta atcgggctgg gcatcagttt tttaccgcgt cctgccggtc
     3661 ggtctggctg ggtgaacacg ccaacgacgt tatgaccaga agacaacagc gcgtcgagat
     3721 gacgcgctgc aaagtcaggt gtacccgcaa aaataatacg tagtgattct gacacgttag
     3781 ttcttatcct taagcccggg ctttcagacg atccagtttt tcaactttct gacgaattcg
     3841 ttgttgtttc agcggtgaca gataatccat aaacagtttg ccaaccaggt gatccatctc
     3901 atgctgaata cagatagcta acagaccctc tgcttccagt tcaaatggtt taccgtcgcg
     3961 gtcaagggcg cgaattttaa ctttctctgc gcgcggcact aaagcacgtt gttcagggat
     4021 cgacaggcaa ccttcttcaa tgcctgtttc gccgcttttt tctaaaagct ccggattgat
     4081 taacaccagc cgttcgtcac ggttttccga aacatcaata acaatgatac gttgatggat
     4141 atcaacctgg gttgccgcca ggccaatacc ttcttctgcg tacatcgtct cgaacatatc
     4201 atcgacgata cgctgaattt ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag
     4261 ccgctcgtcc ggaatatgta acacttgcaa aactgacata aatctccaga gatgtgttca
     4321 ggagttagaa agattatttc ttctattcta gacaaatccc cctctgattg acagcatcac
     4381 ttgattgaca gcatcactga ccaatcgcaa agattgctaa ggcttgctta tgacagggag
     4441 ataaggatgg tcgatacaga aatttggctg cgtttaatga gcatcagcag cttgtacggc
     4501 gatgatatgg tccgtatcgc tcactggctg gcaaaacagt cgcatattga tgcggttgta
     4561 ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt tccacgaaag
     4621 agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat ccctgcggac
     4681 agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc actgtttgtt
     4741 gaaggagaac tgcacgcgct gcattcattt cagcttgccg tagtggggag tcgggcgcat
     4801 tcatggtatg gcgagcgatg ggggcgatta ttttgcgaaa ctctggcgac gtgtggagtg
     4861 acaattacga gtggactggc gcgtggaatc gatggtgtgg tgcataaagc ggccttacag
     4921 gtaaatggcg tcagcattgc tgtattgggg aatggactta atatcattca tccgcgtcgc
     4981 catgcccgac tggctgccag tctgtttgaa cagggtggcg ctctcgtctc ggaatttccc
     5041 ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat cagtggtcta
     5101 agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctggt gacagcacgt
     5161 tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg gaatccggga
     5221 agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga accggaagaa
     5281 attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga aaattcattt
     5341 tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc taacgtagga
     5401 gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt gccagaggta
     5461 gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc cggcggctat
     5521 gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg aaacctatat
     5581 tcacactgaa gctgagttgc gtgtggatca agacaaactt gaacaggatc ttaccgacgc
     5641 aggttttgag cgagaagata tctacaatgc cctgctatgg ctggaaaaac ttgctgatta
     5701 tcaggaaggg ttggcagaac cgatgcaact ggcctcggat cctctctcca tgcgtattta
     5761 tacaccggaa gagtgtgaac gactggatgc cagctgccgt gggtttctgc ttttccttga
     5821 gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag tgctcgcgct
     5881 ggataacgca gagttcgaac tggatgatct gaaatgggtg atcctgatgg tgttgttcaa
     5941 catcccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg aagtgaatga
     6001 aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggca aaatcagcac
     6061 tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg gttattcgat
     6121 ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac tacgtccgtc
     6181 ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag gtttgccccg
     6241 tatgtggcgc aaatctggta ttacgccagg gacgctttgg tatgtttatt ggttgcagta
     6301 actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca gcaattacat
     6361 gcccccaatg tcggacgggc catctggtcc agcgccgctc ccgttatggc aaaacatttc
     6421 actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaacca atagctggag
     6481 aatgccctga gtgtcattat ccgctactca tcgaaaagaa aaccgcgcag ggtgtaaaac
     6541 acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa cgtgaataat
     6601 aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaaa acgtgtcatc
     6661 gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga aacagcagtg
     6721 atgagactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt aatcgcggca
     6781 aattacgagc agcttaaacc ctatattgat gacaccatgt tgactgacgt gcagcgtgaa
     6841 accatttttt cctgctggcc aggtcctgtc acctttgtct ttcccgcgcc tgcgacaaca
     6901 ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga ccatccgttg
     6961 gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc caacttgagt
     7021 ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc ggcgttcccg
     7081 gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga tgccctgacg
     7141 ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg gtaatccgat
     7201 agcccacagc aaatcgccat tcattcatca gcaatttgct cagcaactga atattgaaca
     7261 tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga acgctttctt
     7321 tcgtgctggt ggtaaaggtg cgaatgtgac ggtgcctttt aaagaagagg cttttgccag
     7381 agcggatgag cttactgaac gggcagcgtt ggctggtgca gttaataccc tcaagcggtt
     7441 agaagatgga cgcctgctgg gtgacaatac cgatggtgta ggcttgttaa gcgatctgga
     7501 acgtctgtct tttatccgcc ctggtttacg tattctgctt atcggcgctg gtggagcatc
     7561 tcgcggcgta ctactgccac ttctttccct ggactgtgcg gtgacaatca ctaatcggac
     7621 ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta ttcaggcgtt
     7681 gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa catccagtgg
     7741 catcagtggt gatattccgg cgatcccgtc atcgctcatt catccaggca tttattgcta
     7801 tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc agcgaggctc
     7861 aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg cctttcttct
     7921 ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg aggaattgtc
     7981 cgcgtgaatc aggccatcca gtttccggac agggaagagt gggacgagaa taaaaaatgt
     8041 gtatgttttc ccgctctcgt gaatggtatg caactgacat gcgcgatctc tggcgagagt
     8101 ctggcgtatc gctttactgg agatacgcca gaacagtggt tagcgagttt tcgtcagcat
     8161 cgctgggacc tggaagaaga agcggaaaac ttaattcagg aacaaagtga agatgatcaa
     8221 ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta attattcgcg
     8281 gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt gacggggcta
     8341 ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact acccgcacca
     8401 atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat cccaaccaaa
     8461 actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt gacatcttcg
     8521 ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac atgcaacata
     8581 ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc tccacgaatc
     8641 acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat cacgacactg
     8701 ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta tgggcgtaaa
     8761 acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg ggcattattg
     8821 gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat tgcagcaaaa
     8881 agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca aaatattggg
     8941 atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg atggtcggaa
     9001 caacgaagag
//
LOCUS       DADLTF010000149         9002 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.151, whole genome shotgun sequence.
ACCESSION   DADLTF010000149 DADLTF010000000
VERSION     DADLTF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9002
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.151"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..923
                     /locus_tag="JN749_003427"
     CDS             <1..923
                     /locus_tag="JN749_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7369713.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="JN749_003428"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="JN749_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HAY7369714.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JN749_003429"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="JN749_003429"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HAY7369715.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="JN749_003430"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="JN749_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HAY7369716.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="JN749_003431"
     CDS             4446..4805
                     /locus_tag="JN749_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HAY7369717.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JN749_003432"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="JN749_003432"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HAY7369718.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="JN749_003433"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="JN749_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HAY7369719.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALDHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="JN749_003434"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="JN749_003434"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAY7369720.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2048 a         2174 c         2430 g         2350 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgccg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ctacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccacgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg ataggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat attttacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaatcaac ataatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcac gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggct
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tttgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gttgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggatcataac tgtactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcacc
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gcgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggct tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tc
//
LOCUS       DADLTF010000150         8914 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.152, whole genome shotgun sequence.
ACCESSION   DADLTF010000150 DADLTF010000000
VERSION     DADLTF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.152"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="JN749_003435"
     CDS             complement(145..2688)
                     /gene="mdoH"
                     /locus_tag="JN749_003435"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HAY7369721.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="JN749_003436"
     CDS             complement(2681..4216)
                     /gene="mdoG"
                     /locus_tag="JN749_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HAY7369722.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="JN749_003437"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="JN749_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HAY7369723.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /locus_tag="JN749_003438"
                     /pseudo
     CDS             complement(5775..7256)
                     /locus_tag="JN749_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JN749_003439"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="JN749_003439"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HAY7369724.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="JN749_003440"
                     /pseudo
     CDS             complement(7826..8137)
                     /locus_tag="JN749_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JN749_003441"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="JN749_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HAY7369725.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8649..>8914)
                     /gene="csgA"
                     /locus_tag="JN749_003442"
     CDS             complement(8649..>8914)
                     /gene="csgA"
                     /locus_tag="JN749_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HAY7369726.1"
                     /translation="NSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNG
                     KNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT         2232 a         2310 c         2112 g         2260 t
ORIGIN      
        1 gcataacgcc ggtattttta ttggctcgtt gtttttgccg gatgtggcgt gaacgcctta
       61 tccgacctac aaaatcttgc aaaatcaata aattgcagga acgatgtagg cctgataagc
      121 gtagcgcatc aggcaactac gtttttattg cgaagccgca tccggtttac gcaatgccag
      181 tggattgagc tttatccctt cgtaataact cacccatgaa gaatatcttt caggggaatt
      241 ccagacgcgg aaatgcagac gggccatcgt caccggatcg cttagcagca ccaggcgacg
      301 atcgcgattc agcttctctg gcgtctcgtt aagcgcctgt tcaacgtggc ggtcacgggc
      361 gatttccagc accttactgg cgcggtgacg cgctgtcgcc attgcggttg ccagagcgtt
      421 aaatgacggg ttaaacactg cgtgcataaa gccatcgtca agggagcgtt ggcgattcat
      481 ctcaaggaac cgatcggtat caaccagcac ctgcggcggt gaatactctt ccgggatcag
      541 gaacagtttc cagcgtttgg tgcgcagacc aacggtggca cggctggaaa tcaccgaaac
      601 aaacggtgac aggatcaacg agaagacaat cggtgccagc cagaacagga aacgcagatc
      661 cagccacgcc atcccaacag cccacactaa ccccaacagc agctgtgagc cgtggcgttt
      721 gaacgcttcg ccccatgaag tggagtcatc atcacgctgc ggtgaattcc acaccacttc
      781 ccagccaagg aacgcgctga caacgaagac cgtatggaac agcatgcgta ccggagccag
      841 cagcacggaa aacagcactt ccagcagcaa cgataatgta acgcgccaga agccgccgta
      901 ttctttcgtt cctttgcacc agataagcaa aatgctcaat aacttcggca ggaacaacag
      961 caccatggtc gaagcaaaaa gtgcaatcgc cagctcagga cgccactgcg gccacaccgg
     1021 gaacaactgc cgtggttgca ggaagtattg cggttcggtc aacgcatgca ctacctgcaa
     1081 tgcagtagag agcgcgagga acataaacca cagcggagcg gagagataag acatcacgcc
     1141 cgtcaggaac accgcacggt gaaccgggtg cataccctta accaggaaca gacggaagtt
     1201 catcaggtta ccgtggcacc agcggcggtc acgttttagc tcatcaagca agttaggcgg
     1261 caattcttca taagaacccg ggagatcgta agcaatccag accccccaac ctgcacggcg
     1321 catcaacgcc gcttctacga agtcatgtga caggattgaa ccggcaaagg aaccttcgcc
     1381 cggcagcgga gccagtgcgc agtgctcgat aaatggtttc acgcggataa tcgcgttatg
     1441 tccccagtag tgcgactcgc caagttgcca gaagtgcaaa ccggctgtaa acagtggccc
     1501 atacacgcgg gtcgcgaact gctgacagcg cgcatacagc gtatccatac cggacgcttt
     1561 cggcgacgac tgaatgatcc cggcgttcgg gttggcttcc atcaagcgca ccaggccgca
     1621 taaacaatca ccggtcatta ccgagtcagc atccagcacc accatgtagc tgtactggct
     1681 gccccagcga cggcagaagt catcgatatt accgctttta cgcttcacgc ggcgacggcg
     1741 gcggcgatag aaaatctgac cttcgccacc gacttcagcg ataagctcca tccaggcttt
     1801 ttgctctgcg acgcagatat ccgggttata actgtcactc agaatgtaga catcaaagtg
     1861 tttggcattc ccggtggctt ttactgattc ccacgttgca cgcaagccag caaaaacacg
     1921 gttcacgtct tcgttacaga taggcatgat caacgccgtg cgatgctccg ggtttaatgg
     1981 ttcatcgcca actgttgacg cagatatact gtatttatcg cgaccaataa gcagttgcag
     2041 gaagcccatc aacgccgtcc agaatccggc ggacacccaa cagaacagta ccgcaaaaag
     2101 gatcaggata ccggtttgca gcatataagg cagaagctgc ataaaggaaa cccacaaatc
     2161 ctgaccaacc atatccatag gattaatcag cgcccaaccc tgataaggaa gaatggtctt
     2221 catataccag gtcgcgacaa cagtttgcgc gagcgtcagg atcaacagaa tgtaacggcg
     2281 gatggtacca acggtacgcc acttttgctc gctctcctgc tcttctttgg tcaaacgagc
     2341 cagatagcgc ggcgtgacat cgcgtccacg caggcgatcc cagaaacggc ctaccgggtt
     2401 ggtacgccac gggtcgggaa acatcgagga gcgttttgct tctggcatcg ccttaagctg
     2461 atcgcgccct tcgtcgtctt taattaactg tccatcagca agtgaatctg gccaggcttg
     2521 ttccagacgc gcctttaccg agccttgcgg ggaatcatcc tcccgcgccc aggtgcggtg
     2581 ttcggcatcc agcgcctgat gaacggcgcg gatatcagtc ttcggcaatg ctgctttctc
     2641 gcttgcggcg atgggcattg cgtcaatgta ctcagttgtc ttattcattg gcaggtaact
     2701 ggtagctcca ggtttcactc aacgtctgat cggcattcac cagcgcagca cgcatttcag
     2761 tggttttctt ggcatctttc actttcacac gcatcaccag acgccagcct ttggtaaccg
     2821 ggttatagcg caccgtgctt tcaactatct caccattatc accaatgctg gtttgcgctg
     2881 tgaccggggt atcctctggc agttttttca tctctgcgcc ggtaaaatcg accacaaagg
     2941 cgatagtacc gtcaggctgg cgaatcaggt tcgactgctt cacatccccc gttgaacgac
     3001 gcgtttgttg cacccatgcg ttatctggcg catgcagttt gtcttcatca cggctgaagg
     3061 tgatggtgta tttaaagttc atctctttac ccggctccgg cagctgatcc ggcgtccagt
     3121 aagcgacgat gttatcgttg gtttcatcgt tggttggaat ttccaccagc tcaacgctgc
     3181 ctttgcccca ctcccctttc ggagtcaccc atgcgcttgg acggagatcg taacgatcat
     3241 cgagatcttc aaagcgggag aaatcacgac ctcgctgcaa cagaccaaag cctcgcgggt
     3301 tttccatgga gaagctgctg accgctaaat gtttcgggtt attcaacgga cgccagatcc
     3361 attcgccatt accagcatgg atagacagac cgttagagtc gtgcaactcc ggacgatagt
     3421 tatttgcagg cgacggttgg ttcggcccaa acaggaacat actggttaac ggtgcaaccc
     3481 ccagtttgcc gactttatcg cgcagataga ttttcgactg cacatccaca accgtgtcac
     3541 gccctggcat aaccacgaat ttgtaagcac ctgtcgcacg cggcgagtca agcaatgcat
     3601 aaatggttaa acgtttatca gtcggttttg gacgctcgat ccagaactct ttgaagcgtg
     3661 gaaattcttc acccgatggc aaagcggtat caattgctag gccgcgggca gaaaggccat
     3721 aaacctgacc tgcaccaatc acgcggaaat agctggcccc gagcatgctg acgatttcat
     3781 cgtttttatc tttgctgttg atcgggtaaa gcactttaaa accggcaaaa ccaaggtctt
     3841 ttaccgtgtc tttgtcatgc tgaacatcgc cgaaagtgaa ataatccggg ctgtatttga
     3901 ttcgtttgac tgcggtggca gtcacttcat ttattttgac cggggtatcg aagtacatac
     3961 cctgatggta gaactcgagt ttgaatgggg tcttcagatt gttccagtac gctttgtcat
     4021 gattaaactg gatctgctga tagtccgcgt atttcatgtc gcggaaaacg gagggcaagt
     4081 tgcttttagg cgcctcatag cctttcccgg ctaaggattg agcttgcttt gcgacatcat
     4141 caatactgaa agcccagctt gaagatgtat acagggttaa cattactgca gcactcaacc
     4201 aacgcatttt catcatttgt agtttatgtt tcataattag taagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa ctgaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca aatttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggatactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatccccc tactgacatt
     4921 accgcaattt attatgctgc aatatgtcaa agggaaagcg gaaagttggc ctgggctatc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgtgt atggatattt aagcgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccacccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt taccgttctt
     5281 tatcctcggc gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt accctcgctg cagcattggc gtttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggcct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgttta cttaacttcc agtcagcgcg
     5521 ggtgacttat tttgttaacg catcgctgtt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttctctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taacctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcgtac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg caccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact gctttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc gaacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgtct tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaatgga ctggattcca ttttgcgtaa ataacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacggggcct attgccatga catctaaatc cgaaaaaagt
     6721 cgctcttccc ctgcactaaa ataggcatca ccaatattta gtcctcccac tagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atccggcgat tgagacggga aaagtcggtg
     6841 atatatccaa gcggacgtag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cgtggatgac tgtcaagcaa gcgtaaaatg tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgccccgaca tatcgtccta ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgtagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gagttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgtttttg acattgccgc
     7921 tcattaacgc tttgctggag taggttttgc ccggaataga accattctat gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac gcccccttgc gcggtgcttc ctgcaatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagtct aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 tccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aatgggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtaac ttcaggaata atggtgtaca
     8521 tatccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaaa cagggcttgc gccctgtttc tgtaatacaa
     8641 atgatgtatt aatactgatg agcggtcgcg ttgttaccaa agccaacctg agtcacgttg
     8701 acggaggagt tcgatgcagt ctggtcaact gcagcaccgt tgccaccacc gaactgttta
     8761 acagtcattt cagaattttt gccgttccac tgatcaagag tggcgctgtt accgaagcca
     8821 cgttgggtca gatcgattga gctgtcatct gagccctgac caacatctgc accattacca
     8881 ccgccatgct gggtaatagt caagtcagag ttac
//
LOCUS       DADLTF010000151         8839 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.153, whole genome shotgun sequence.
ACCESSION   DADLTF010000151 DADLTF010000000
VERSION     DADLTF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.153"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(158..1129)
                     /locus_tag="JN749_003443"
     CDS             complement(158..1129)
                     /locus_tag="JN749_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HAY7369727.1"
                     /translation="MKRAPVIPRHTTHSQSTEDTSSPAPAAPMVDSLIARVGAMARGN
                     AISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSDVIEIADGSRRRKAAILTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSA
                     RSGEALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSPASRVNLS
                     SRHQFAPGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKPGV"
     gene            complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN749_003444"
     CDS             complement(1129..2295)
                     /gene="sopA"
                     /locus_tag="JN749_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HAY7369728.1"
                     /translation="MGLMDTLNQCISAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DAFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPA
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3036..4046
                     /locus_tag="JN749_003445"
     CDS             3036..4046
                     /locus_tag="JN749_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7369729.1"
                     /translation="MTSENNSLLLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLF
                     VPTLKSTGKSKANRKNVTDATEELVQLAIAKSEGYTDVKITGSRLDMDTDFKVWLGII
                     RSMSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLKFQSETK
                     GWTTHLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYT
                     YIESLPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRAT
                     YFSVHYRNPKLISSPVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPLKLAEALSAMKP
                     EN"
     gene            complement(4852..5181)
                     /locus_tag="JN749_003446"
                     /pseudo
     CDS             complement(4852..5181)
                     /locus_tag="JN749_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5299..6024
                     /locus_tag="JN749_003447"
     CDS             5299..6024
                     /locus_tag="JN749_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7369730.1"
                     /translation="MAYSIGEFARLSGITATTLRAWQRRYGLLKPQRTEGGHRQYSDE
                     DVQQALKILDWVKKGVPIGQVKPLLERPTSRLTNNWLTLQQSMLQRLQDGKIESLRQM
                     IYDAGREYPRPELVANVLRPLRSQVSANVAAAMTLREILDGIIIAYTSFCLEGDKKAP
                     GDNILLSGWNLNDPCEVWLEALTRTGQGHRIDILPTPPATLAPEIFPDRHWLLVTRGK
                     LTAARKKQIEQWQQQVSLEVIIL"
     gene            6124..6534
                     /gene="uraH"
                     /locus_tag="JN749_003448"
     CDS             6124..6534
                     /gene="uraH"
                     /locus_tag="JN749_003448"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7369731.1"
                     /translation="MKKTASLLMLTTLAFAPAAFSAPAGTLSVHILDQQTGMPPSDVT
                     VTLEKQQQDKWTPIASGKTDHDGRIKSLYPEDQDMQPGVYKVAFKTADYFHGKKLDSF
                     FPEIPVLFTVTRTNEKLHIPLLLSQYGYSTYKGS"
     gene            6637..7596
                     /locus_tag="JN749_003449"
     CDS             6637..7596
                     /locus_tag="JN749_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HAY7369732.1"
                     /translation="MTTKPVLGISGCLTGSAVRFDGGHKRMGFVMDELAQWVSFRPVC
                     PEMAIGLPTPRPAIRLTLTDSGETQLRFSKPPHDDITQKMADFTADYLPKIGDLSGFI
                     VCAKSPSCGMERVRLYDENGNRGRKEGVGLFTAALLETYPWLPVEEDGRLHDPVLREN
                     FIERVFALHELNTLRAKGLTRRALLDFHSRYKLQLLAHHQAGYREIGPFVASLHEWED
                     LDAFFVAYREKLMTILKKPASRKNHTNVLMHIQGYFRNQLNSRQRVELRDVILHYRDG
                     LLPILAPLTLLKHYMAEYPDRYLMTQNYFDPYPDDLGLRLAVT"
     gene            7742..8830
                     /locus_tag="JN749_003450"
     CDS             7742..8830
                     /locus_tag="JN749_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7369733.1"
                     /translation="MITTLHIQNYRSIREMSLELEQLNIVFGPNGTGKSNIYKAIHLM
                     HSAAQGQFSQALANEGGILKVFWAGKTRSDQLRRMNLAVETETYEYELQVGFVEKLPY
                     PSQFQLDPVIKEESIWLSGQYRRPSSQLMKRKNQAVFLNNVHHEKVTHSGTLYENESV
                     FGQLGEPHLYPEVSQMRESLRNWRFYQEFSVSIGSAMRAPQVGFRSPVLASDGANLAA
                     AFQTIVEIGDELLLMRILDQAFPGCVFYSDNTGGRFRMMMQREGLSRPLEPAEFSDGT
                     LRFLCLAVALLSPRPPAFIALNEPENSLHPQMLPALASLIAEASRYSQIWLTSHSPEL
                     ANLIEKQRSFSLYQLSMVEGETKVERLG"
BASE COUNT         2235 a         2336 c         2254 g         2014 t
ORIGIN      
        1 cgacgcaggt gggaccgtgg tcccagggag ataacctgag cggcgataca ggtgggaccg
       61 tggtcccaga gagaatattc aggccagtta tgttttctgg cctgtgagaa aggaaatgaa
      121 gtaaagggag atcaccgtag actaaagcgt gtccgcatca gactcctggt ttctcaagct
      181 ccttaagaat ggcttctatt ttctctatac actccgcagg aatgcgggac ctatccaggt
      241 taagcaccat tttatcgccc ttatacaatg ctgttgcccc tggagcaaac tgatgacgtg
      301 agctcagatt aactcttgat gcgggagatt gtttcagtac ggatgttaaa agactaatga
      361 cctcttcagc ttcaaagatt agtcccgctt tcttctgatc gtggagagtc tcagcctgct
      421 gcttcagtaa ttcttcttta tctgcaaaag ccttctgaag ggcttcacct gaccgggcag
      481 acagttctcc aggatgtgca aacagggcaa caacggattt aggcaatttg gccgtattga
      541 tacagcgggt aatgatctta cgagagatgt tttccgcatc ggcaagcgca gaaatatttc
      601 ctgcaaactc attctgcagc cggcttgtat aacgcaggcc acgttcataa gcactcgtcg
      661 gccgataatc gttacccagt cgggacaatg cagccatctg ctcatcgtcc agttcaccaa
      721 ccagaacgcg ataatcactt tccgtcagga ttgcggcttt acggcgacgg ctgccgtcgg
      781 caatttctat gacatcagaa acccggcggc cgaaagctgg agtctgctga ccggtcagta
      841 aaaatgaagg aatgagatca tccagagcat cttcggtaag tagttcctga tcgcgctcat
      901 tgcctgacca tacgcgtgaa gcgctctcaa cagagtctcc ccggagcacc tcaagggtaa
      961 atttcacttc ccgtccacat accggaagag agattgcatt accgcgggcc atagcaccca
     1021 cgcgcgcaat taatgaatcc accatcggcg cggccggcgc cggtgatgaa gtatcttcag
     1081 tcgattgact atgtgtggta tgtcttggaa tgacaggagc acgcttcatt atctaatctc
     1141 ccagcgtggt ttaatcagac gatcaaaaat ctcattgcaa acaggctccc agatagagag
     1201 ggcattacgc caggcaccgg ttgaagaacg ttgatcaata gcctgttcaa aaacggttct
     1261 catacggatc tgacctttac cgacttcatc cgtttcgcgc acaacatttt taaggaccat
     1321 gcttccccag gcatcccgaa tttgttcttc catccacggg gactgtgagc cattattatt
     1381 actgtatttg gtaagcagaa tacggacatc tggctcgaag cctttaagat ccacgttctt
     1441 aagcaggtca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcga acagctctgc
     1501 cggagtaggg acaatcagaa catcagcagc gcatacaaca tttatcgtac cgataccaag
     1561 gttgggtgca ctgtcgataa ctatcacgtc ataatcatga gcgacagttt caatggccag
     1621 acggagcatc aggtgcgggt cagcaggtaa tttgccttcg tcaaatttcc ccatcagctc
     1681 ggtttcaatg cggtgcagtg ccagacagga cgggatgata tcaaggccag gccagcaggt
     1741 gggctttata gcgtagctgg cgtcatcctt ttccccaaga taaaagggca ggagagtgtc
     1801 ctcagcatga atatgaagat cgggcaccca tccgtgatac atagaagcag ttccctgtgg
     1861 gtcattgcct tcaacaagca gaacacggag tcctttcaga gcaagatcct gagccaggtg
     1921 aacggatact gaggttttgt aaacaccacc tttgtgagct gcaacgccaa tcaccggcgg
     1981 aaacgcatct tcagcacggc gcagtcgtgt accaaataca tcccgcatgt gattgatttg
     2041 ttcgatggta taaccaacgc gttgctcaac tctcccacgg gtttccatat cagggtgcgg
     2101 cagccggcca gctttctcgg catccctgat agcctgagat gacacgccaa ctaaatctgc
     2161 cgcttcacca atcctccagc gccgggttat tttccgggct tccggactgt catcattaaa
     2221 ctgtgcaatg gcgattgctt tcgtcatttc atgaccagca cttatgcact ggttgagtgt
     2281 atccatgagt cccatactga acatccttta atcatcactt tgcattattt tattaaatct
     2341 tggaatttat tgcaaagcaa caataaaatc gcaaagcaca cgaaaaagtg caaagtgaac
     2401 cacattgggt aatgaaagaa cgcaggggat tacaaaagag tacataccgc agtcaatatt
     2461 aatcactgcc gctcgccgca gtaagcgatc tcacacagtg agaaagcggc gaggaagcgg
     2521 aaaatcacag aactcacaga ttgcacgtac gtcgggttgc taaagatgac tcgggatcag
     2581 ccagttataa tgaaattgac tgcggtatgt aatatattga ctatggtatg atgcttaccg
     2641 catctgtagg aaagacgact cagcataccg cagtcaatgt ttcattgttg tacctgtaga
     2701 tctcaatctt ccagagcgta ttcaggaaaa tcaggccagg ggtctggata tctggtgtgg
     2761 cgcttccggc cttgtatctg ggttattcat ggtctcgatc caataattga tcccaaggag
     2821 atcatagtga gatccaaaag agatccccgg atcgctccag catgcgctgt aactggcttt
     2881 aacgtctgtc tggatcgact gcggaatgta aaatgttgac tgttatatgc ataatctaat
     2941 actgtgatat gcaacggtat ttactgaggt atgtatatcc gttgactgcg atacgtatta
     3001 ctacatcctt ttgtggataa ccgttgagga tcaagatgac gagcgaaaat aatagcttac
     3061 ttctgaacct tcaggaagtt gataagacaa ccggtgaagt tgttaagctg gatgtcaaca
     3121 gcaccagtac cgtgcagcca gtagcgctca tgagactcgg tctcttcgtc ccaactctga
     3181 aatcaacagg gaagagcaag gcgaaccgga aaaacgttac agacgcgact gaggagcttg
     3241 tacagctggc cattgccaaa agcgaaggat acactgacgt taagatcacc ggttcgcgtc
     3301 ttgatatgga cacggatttt aaagtctggc tggggataat tcgctccatg tcggagtatg
     3361 gggtaaaaag tgacaccctg gaactgtcgt tcgtcgaatt cgttaagatg tgcggatttg
     3421 actcccgtcg ttcaaacaaa aaaatgcgcg atcgcatcag taattccctg tttaaactcg
     3481 cctcggttac gctgaagttc cagagcgaga ccaaaggatg gaccacccat ctggtgcagt
     3541 cagcctatta tgacatcaac gaggatatcg ttgagatcaa ggctgaacct aagctgtttg
     3601 agctttacca catggacaga agggtactgc tgcggctgaa agccattgac gccctgcagc
     3661 gcaaggaatc agcccaggcg ctttatacct acatcgaaag tcttccccag aatcctgcac
     3721 ctatttccat gaaacggatg cgtgaccggc tgaatctcac ctcaaatgtc tatacacaga
     3781 accacacggt gcgtaaggcg atggagcagc tgcgtgatat tggttatctg gattacaccg
     3841 agtttaaacg tggccgagcc acctatttca gcgtgcatta tcgcaacccc aaactcatca
     3901 gtagtcctgt gaaggttccg cgcaaagagg aagaagagaa ggcgccagag caaaattatg
     3961 acgaagtcat taaggcgctg aaggctgctg gcatcgatcc tcttaaactg gctgaagccc
     4021 tctcagcgat gaagccagaa aattgactgc gatatgcata tcatgactgc cgtatgccgc
     4081 taaggacatt ccattgactg tgatatgcat cacgcatacc gcagtcaata tttattctct
     4141 atcatgtctg cccggatcga acaatattga ctgtcatatg tgatttcggg tcatgatacg
     4201 cagatattga ctacggaatg cgggtttaat gggggtaatc cctggtctgt tcaccattcc
     4261 atgggggata acatgccaca gtcaattttt cagtaacgct ctatatccct tacgaaaatc
     4321 tcaatcgggt gctgagacgg tcatgttacc tgacggtatc cgttcccctg tgtactgcag
     4381 gtttagcccg gcaggatgag actgcagctg tctgtataac ccggcgccag cttcacactg
     4441 ttaaatgcga tcaggaaacg ggagccgaat ggcagaaagg tgttggctag ggaaggtgcg
     4501 aataagcagg ttatttcttc ccaagctgac tcgctgatta aaatttcgcg gatctgggcc
     4561 gatttttttc ccgcaaacac atcgaatcag cctatttagg ggtcagtttg gagatcggaa
     4621 atttttgtac gttaagcaca ttatttaacc aattcggata cgtaagtgat tgatttatcg
     4681 tcattggcta tcgcttttag gttgtcgctc ttgctgtatc gcatttaggt tatccgatta
     4741 agacaaaatc ccctgaaaac gctgtatagc gcgggagtac accctattgc tacaggtaag
     4801 cctgttcaaa aaacaggctt accgtacaat aattctctat atccaaactg accccaagaa
     4861 ggacaccttc agcgcggccg tggtcggttc gccggtaaag taggctttac cctcgctctc
     4921 attccacttg ttcttcacag ggtcgtagcc gcggttaagc acgctgatgc cgccgtcgct
     4981 gcgcttgccg taggtggcgg taacctgctc taggccgcgt tcaaagcggt tttccaggcg
     5041 agcgacttcg taccatttgc caagatagcg gctggcgtca aaaccggaaa tgggctgcac
     5101 gcccttcggt ggggtagggg atttacaggc taccagcgtc agggcaatgg caacaccggt
     5161 cacaacaggc catagcttca tgagaattcc tttcatttgc acggttggaa attaagtgta
     5221 gtgcaccgcg gcggcgcttc atcccatccg cgaaaattcg tagtatattg agagtattcc
     5281 tcaactctgg acacggacat ggcttactcc atcggcgaat tcgccagact cagcgggatc
     5341 accgccacca cgctgcgggc ctggcagcgg cgctacggct tactcaagcc tcagcgcacg
     5401 gagggcggcc accgccagta cagcgatgag gacgtccagc aggcgctaaa aatcctcgac
     5461 tgggtaaaaa aaggcgtccc gattggccag gtcaaaccgc tgctggagcg ccctacgtcg
     5521 cgcctgacca acaactggct cacgctgcag cagagcatgt tacagcgcct gcaggacggc
     5581 aaaatcgagt ccctgcgtca gatgatttac gacgcaggac gcgagtaccc aagaccagaa
     5641 ctggtcgcga acgtgctgcg tccgctgcgc agtcaggtgt cggcaaacgt cgccgccgcc
     5701 atgaccctgc gggagatcct cgacggcatc atcatcgcct acacctcgtt ttgcttggaa
     5761 ggcgataaaa aagcgccggg cgacaacatc ctgctcagcg gctggaacct gaatgacccg
     5821 tgcgaagtct ggctcgaagc cttaacccgc accgggcaag gccaccgtat cgatatttta
     5881 cccacgccgc ccgccacgct ggccccggag attttcccgg accgccactg gcttctggtc
     5941 acgcgcggta aactcaccgc cgcgcgcaaa aagcagatcg agcagtggca gcagcaggta
     6001 tcgcttgaag tgattatcct ctaatcattt tctcctgcgg gaatcttcac gaaaagttga
     6061 atattttccc gcgccacaaa tgctaacgtt ttcctgtgac cgacaacccc acaggaaaac
     6121 acaatgaaaa aaactgcctc tctgctgatg ctgaccaccc tggcctttgc ccctgccgcc
     6181 tttagcgccc ctgccggcac gctgagcgta cacattcttg accagcaaac cgggatgccg
     6241 ccatcggacg ttaccgtcac gctggaaaaa cagcagcagg acaaatggac cccaattgcc
     6301 agcggcaaaa ctgaccatga cggccgcatc aaatcgcttt atccggagga tcaggatatg
     6361 cagcccggcg tctataaagt ggcctttaaa accgcagatt acttccacgg gaagaagctg
     6421 gactccttct tcccggagat cccggtgctg ttcaccgtaa cccggaccaa cgaaaagctg
     6481 cacatccctc tcctgctgag ccaatacggt tattcaactt acaagggcag ttaagaaacg
     6541 ttgattttaa aggcaaataa aaaataattc cgctaatact tggacaaatt agttatgttg
     6601 tgtaagtttt aaagagaaga acgggaggac cacaccatga caacaaaacc tgtgctcggt
     6661 attagcggat gtttgaccgg gtctgccgtt cgcttcgacg gcggacacaa gcgtatgggc
     6721 tttgtcatgg atgaactggc ccagtgggtg agtttcaggc ccgtctgccc ggaaatggcg
     6781 attggcctgc cgacgccacg cccggccata cgcctgacgc tgacggacag cggcgaaacg
     6841 cagctccgct tcagcaagcc gccccatgat gatataacgc agaaaatggc cgactttacg
     6901 gcggactatc tgccaaaaat cggcgatctg tcgggattta tcgtctgcgc gaaatcgcca
     6961 agctgcggca tggaacgcgt gcggctatat gatgaaaacg gcaaccgggg ccgcaaggag
     7021 ggtgtcgggc tgttcaccgc cgccctgctt gaaacctacc cgtggctgcc cgtggaggaa
     7081 gacggtcggc tgcacgaccc ggtgctgcgg gaaaacttta tcgagcgggt atttgccctg
     7141 cacgagctga acacgctgcg cgctaaaggc ctgacgcgcc gcgcgctgct ggatttccac
     7201 agccgctata aattgcagct tctggcgcac catcaggcgg gctaccgcga gattggcccg
     7261 ttcgttgcgt cgctgcatga atgggaagat ctggacgcgt tcttcgtggc gtaccgcgaa
     7321 aagctgatga ccatcctgaa aaagcccgcc tcgcggaaaa accataccaa cgtgctaatg
     7381 catatccagg gctatttccg taaccagctg aacagccgcc agcgcgtcga gctgcgcgat
     7441 gtgatcctgc actatcgcga cgggctgcta cccatcctcg cgccgttgac gctactgaaa
     7501 cactacatgg ccgaataccc tgaccggtat ctgatgacgc aaaactactt tgatccctat
     7561 cctgatgatt tgggtctgcg tctggcagta acctgacgat aaaaatgcga aggcccgttt
     7621 gacccacaga ggcccaaagc agtaccccgt ttagacgaca ccctcactgt cgtctctttt
     7681 ttgcattcca cacaaaagac tgtaattatg tacaggattg taacgcaaaa agggactgcc
     7741 tgtgatcacc accctgcaca ttcaaaacta ccgctcgatt cgcgaaatgt cgctggagct
     7801 ggaacagctc aacatcgtct ttggcccaaa cggcaccgga aaatccaata tctacaaggc
     7861 gatccacctg atgcacagcg ccgcacaggg gcagttttcg caggcactgg cgaacgaggg
     7921 tgggatttta aaagtgttct gggcaggcaa aacccgcagc gaccagctgc ggcggatgaa
     7981 tttggccgtt gaaaccgaaa cctacgaata cgagctgcag gtaggctttg tcgaaaaact
     8041 gccttacccc tcacagtttc aactcgatcc ggtgatcaaa gaagagtcca tctggctgag
     8101 tggtcaatac cgccgcccgt cgtcacagct gatgaagcgt aaaaatcagg ccgtatttct
     8161 gaacaacgtg catcacgaga aagtcaccca cagcggcacg ctgtatgaga acgaatcggt
     8221 gttcggccag cttggcgagc cgcatcttta cccggaagtg tcgcagatgc gcgagtcgct
     8281 gcgtaactgg cgcttttatc aggagttttc tgtatcaatc ggctcggcca tgcgcgcgcc
     8341 tcaggtcggt ttccgctccc cagtgctggc cagcgacggc gcaaacctgg cggcggcgtt
     8401 tcagaccatc gtggaaattg gcgacgagct gctgctgatg cgcatcctcg accaggcctt
     8461 ccccggctgc gtgttttaca gcgacaacac cggcggacgc ttccggatga tgatgcagcg
     8521 tgaagggcta agcagaccgc tggagccggc ggaattctcc gacggcactc tgcgttttct
     8581 gtgcctggcg gtggcgctat taagcccgcg tccaccggcc tttatcgccc tcaacgaacc
     8641 ggaaaacagc ctgcatccgc agatgctgcc cgcgctggcg agcttaatcg ccgaggccag
     8701 ccgctactcg caaatttggc tgaccagcca ctcgccggaa ctggcgaatt taattgagaa
     8761 acagagatcg ttttcgctgt atcagctgtc gatggtggaa ggagaaacga aggtggagag
     8821 gctggggtag tctcccaga
//
LOCUS       DADLTF010000152         8711 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.154, whole genome shotgun sequence.
ACCESSION   DADLTF010000152 DADLTF010000000
VERSION     DADLTF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.154"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JN749_003451"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="JN749_003451"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HAY7369734.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..2886
                     /gene="cpdB"
                     /locus_tag="JN749_003452"
     CDS             943..2886
                     /gene="cpdB"
                     /locus_tag="JN749_003452"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HAY7369735.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(3012..3392)
                     /locus_tag="JN749_003453"
     CDS             complement(3012..3392)
                     /locus_tag="JN749_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7369736.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            3481..4341
                     /gene="qorB"
                     /locus_tag="JN749_003454"
     CDS             3481..4341
                     /gene="qorB"
                     /locus_tag="JN749_003454"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HAY7369737.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            4450..5415
                     /locus_tag="JN749_003455"
     CDS             4450..5415
                     /locus_tag="JN749_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7369738.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            5523..6185
                     /gene="ytfE"
                     /locus_tag="JN749_003456"
     CDS             5523..6185
                     /gene="ytfE"
                     /locus_tag="JN749_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HAY7369739.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="JN749_003457"
                     /gene_synonym="dagA"
     CDS             complement(6230..7642)
                     /gene="cycA"
                     /locus_tag="JN749_003457"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HAY7369740.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(7950..8570)
                     /locus_tag="JN749_003458"
     CDS             complement(7950..8570)
                     /locus_tag="JN749_003458"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="FKBP-type; rotamase; catalyzes the cis-trans
                     isomerization of proline imidic peptide bonds in
                     oligopeptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAY7369741.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALIAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         2211 a         2295 c         2207 g         1998 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga aaggattaaa agcaggtgat gttcatccgg tttataaggc gctgaatacg
     1261 ctggattata cggtcggtac actcggcaat catgaattta actacggtct ggattacctg
     1321 aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg ccaacgtcat tgacgccaga
     1381 accaaacagc caatgtttac accgtattta attaaagata ccgaagtggt cgataaagac
     1441 ggaaaaaaac agacgctgaa gattggctat attggcgtcg tgccgccgca aatcatgggc
     1501 tgggataaag ctaatttatc cggaaaagtg acggtgaatg atattaccga aaccgtgcgc
     1561 aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtca ttgtcgttct ggcgcattcc
     1621 gggctgtctg ccgatccgta taaagtaatg gcggaaaact cagtttatta cctcagtgaa
     1681 attccgggcg ttaacgccat tatgtttggc catgctcacg ccgttttccc gggtaaagat
     1741 tttgctgata tcgaaggggc tgatatcacc aaaggcacgc tgaatggtgt tccggcggta
     1801 atgccgggca tgtggggcga tcatcttggg gtggtcgact tacaactcag taatgacagc
     1861 ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc cgatttacga catcgccaat
     1921 aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa cactcaaagc cgatcacgat
     1981 gccacacgcc agttcgtcag caagccaatc ggtaaatctg ccgacaatat gtatagctat
     2041 ctggcgctgg tgcaggacga tccgaccgtg caagtcgtga acaacgcgca aaaagcgtat
     2101 gtcgaacatt acattcaggg cgatccggat ctggcaaaac tgccggtgct ttcagctgcc
     2161 gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa gctatgtgga ggtggaaaaa
     2221 ggccagttga ccttccgtaa tgccgccgat ctttatctct accccaatac gctgattgta
     2281 gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt gctccgccgg acagtttaac
     2341 cagattgctc ctaacagcac gaaaccacag tcactcatca actgggatgg tttccgcact
     2401 tataactttg atgttattga tggtgtgaat tatcagattg atgttaccca acccgcccgc
     2461 tatgacggcg agtgccagat gattaatgcc aatgcagagc ggattaagaa cctgaccttt
     2521 aatggcaaac cgattgatcc gaacgctatg ttcctggttg ccaccaataa ctatcgcgct
     2581 tacggcggca aatttgccgg tacgggcgac agccatatcg cttttgcttc accggatgag
     2641 aaccgctcgg tgctggcagc gtggattgct gatgagtcga aacgtgcggg ggaaattcat
     2701 ccggcggcag ataacaactg gcgtttagca ccgatagctg ccgataagaa actggatatc
     2761 cgtttcgaaa cttccccgtc agataaagcc gcagcgttta ttaaagagaa agggcagtat
     2821 ccgatgaata aagtcgcgac cgatgatatc gggtttgcga tttatcaggt ggatttgagt
     2881 aagtaaaaca cttctttttc ggcctataaa tcaaccgcat ccggcattta ttggcggatg
     2941 cgatgctgcc gtatcttatc caccctacaa gccatgaacc gtagaccgga taagcgcagc
     3001 gcatccggca gttatgccgc acgttcatcc cgcaccgcca acacctcggg caaattcaac
     3061 tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag cggcgtgagg
     3121 ctatattcca catgcggcgg caccaccgga tacgcgatac ggttaagaaa accatcctgt
     3181 tctaacgcct gtaacgactg cgcaagcatc ttttcactca ccccaccgat tttgcgccgc
     3241 aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac cccccaacgg
     3301 ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag attaccctct
     3361 ttcagttgtt gtgacaggct aacctgactc atttcatact tacctttttg cacgtactta
     3421 ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga caaaacgatg gagacttccc
     3481 atgatcgcta ttactggtgc cactggccaa cttggtcact atattattga atccttgatg
     3541 aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa agcccaggcc
     3601 ctggcagcac aaggcattac cgtgcgtcag gctgactaca gcgatgaagc cgcactgaca
     3661 tctgcacttc agggagtgga aaaactactg ctgatctctt ccagcgaagt gggtcaacgt
     3721 gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ctggcgtgaa atttatcgct
     3781 tataccagcc tgctacatgc agatacctcc ccgctcggcc tcgccgatga gcacatcgag
     3841 acggagaaaa tgctggctga ttctggcatc gtttacaccc tgctgcgcaa cggctggtac
     3901 agcgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt tatcggtgcg
     3961 gcgggcgatg gcaaaatcgc ctcagcaacg cgggcagatt atgcgacagc tgcggcactc
     4021 gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga tagtgcctgg
     4081 acgttgacac agttagcggc agagctgacc aaacagagcg gcaaacaggt tacctatcaa
     4141 aatctgagcg aagccgattt cgccgcggca ctgaaaagcg tcggactgcc cgacggactg
     4201 gcggatatgc tggcggattc tgacgttggc gcatcgaaag gtggtctgtt tgataacagc
     4261 aaaacgctta gcaaattgat tggccgccca acgacaacgt tagccgaaag cgtaagccat
     4321 ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct ctctgataat
     4381 gacgggatgc cgggagcaat catgtctgct tcctgaacgt ttcttctgac agaccaatgg
     4441 atgccagtaa tgattagcgg cgtgctgtac gccctgttag cagggttgat gtgggggctt
     4501 atttttgtcg ggccgttgat cgtgccggaa tacccggcga tgttacagtc gatggggcgt
     4561 tatctggcgt tagggttaat tgcgctaccc attgcctggc tgggacgcgt gcgtctgcgt
     4621 cagttggcgc gtcgggactg gcttaccgcc ttgatgctca ctatgatggg caacctcatt
     4681 tattacttct gccttgccag tgccattcaa cgtactggcg cgcctgtttc cacgatgatt
     4741 atcggcaccc tgccggtggt gattcctgtc tttgccaatc tgctttatag ccagcgcgac
     4801 ggcaaactcg cgtggggaaa actcgccccg gcactgattt gtattggcat cggcctggcg
     4861 agtgtgaata ttgctgagtt aaaccacgga ctccccgatt ttgactgggc acgttatacc
     4921 tctggcatcg tgctagcgtt agtttccgtg gtctgctggg catggtatgc cctgcgcaac
     4981 gcccgctggc tgcgggaaaa tcccgacaaa catccgatga tgtgggcgac ggcgcaggcg
     5041 ctggtcacgc tgccggtttc tctcatcggc tatctcgtcg cctgttactg gctgaatata
     5101 caaacgccgg acttctcctt accttttggc ccccgtccgc tggtgtttat tagtctgatg
     5161 gttgcgatag ccgtgctttg ctcatgggtt ggcgcactct gctggaacgt cgccagccag
     5221 cgattaccga cagtgattct cgggccgctg attgttttcg aaacgctggc aggtttgctg
     5281 tacacctttt tactccgcca gcaaatgccg ccgctaatga cgctgagcgg tatcgcgctg
     5341 ttagtgattg gcgtggtcat cgcggtcaga gcaaaaccag aaaaaccttt aactgaatct
     5401 gtctcagaaa gttgacatgc tggcagtgag ttaaataagc ctctgctacg taagggttat
     5461 agcttttgcc ttaaaggtgt atttaaaata catcttatct tattaagaat gaggtatcag
     5521 ctatggctta tcgcgaccaa cctttaggtg aactggcgct ctctatccct cgcgcttcgg
     5581 ctctgtttcg taaatatgat atggattact gctgtggcgg taagcagacg ctggcgcgcg
     5641 cggcggcacg taaagaactg gatgttgagg tcattgaagc tgaactggca aagcttgctg
     5701 aacaaccgat tgagaaagac tggcgtagcg ccccgctggc agaaatcatt gaccatatca
     5761 tcgtgcgcta ccacgatcgt caccgcgagc agctgccgga gctgatcctg caagcgacta
     5821 aagtcgagcg cgttcacgcc gacaaaccga gcgtgccaaa agggctgaca aaatacctga
     5881 ccatgctgca tgaagagctt tccagccaca tgatgaaaga agagcagatc ctcttcccga
     5941 tgatcaaaca aggcatgggc agccaggcaa tggggccaat cagcgtaatg gaaagcgagc
     6001 acgatgaagc gggcgaactg ctagaagtga tcaaacacac taccaataac gtcacaccgc
     6061 cgccagaagc ctgcaccacc tggaaagcga tgtataacgg cattaatgaa ctgattgatg
     6121 acctgatgga tcacatcagt ctggaaaaca atgtactgtt cccacgcgcc ctagcgggtg
     6181 agtgatctaa aagctggatg gcattgcgcc atccagcatg ataatgcggt tatttccgca
     6241 gttcagcagc ccgcttctta ccaataaaca gccagcccaa ccccagcgcg ataaaccaca
     6301 gcggggtaac cagcagcgcc tggcgagtgt catcttccag tgtcagcaac acgaccacga
     6361 acacaaagaa cgccatacat acccagcaca tcagcttgcc gagcggcatc ttgtagatcg
     6421 acttttcatg cagatgagga cgctgtttgc ggtacaccag gtacgagcaa aggataatcg
     6481 tccagacgaa cataaacaga atcgcggaaa cggttgtaat catcgtgaac gcgccaatca
     6541 cgctaggatt cacatacaac atcaccacgc cgccgagcag acagatacac gagaaggtca
     6601 accctttcgc gggtactgcg cgcttagaaa gtttagcgaa cgctttcggc gcaacacctt
     6661 cctgcgccag accaaacagc atacggctgg tagagaagac accgctgtta gcggaagacg
     6721 ccgcagaggt cagcaccaca aagttgatca cgctggcagc cgcaggcaaa cctaccaaca
     6781 caaacagctc aacgaacggg cttttctccg ggactaccga actccacggt gtcacggaca
     6841 taatcacaat cagcgcgaag acgtagaaca taatgatacg gatcggaatg gagttaatcg
     6901 cgcgtggcag tgatttctcc ggatctttgg tttccgcagc tgttgtacct accagctcaa
     6961 tccccacgaa agcgaaaacc gctatctgga atccggcaaa gaagccactt aaacctttcg
     7021 ggaaccagcc gccgtcattc cacaaatgcg cgaatgacgc ttccacaccg gtcggtgact
     7081 gaaagtgcat cgccaccatg accaggccga cgacaatcag cgacacgatg gcgacgattt
     7141 tgatcatcgc aaaccagaac tccatctcac cgaacatttt cacggtggca aggttgagcg
     7201 tcagcagcag cactatcacc gccagcgagg cgacccagtc ggagaaatcg gggaaccaga
     7261 actgagcata agccgtgatc gccaccacgt ctgccatacc ggttacaacc cagcagaacc
     7321 agtaagtcca gccggtgaaa tatcccgccc acggccccag taaatcggaa gcgaagtcac
     7381 taaaagattt gtattccaga ttcgaaagca gcaattcccc cattgcccgc atcacgaaaa
     7441 agagcataaa accaatgatc atataaacga aaatgatcga cggcccggca aggctaatcg
     7501 ttttgccgga ccccataaac aacccagtac caatggcacc gccaatggca ataagctgaa
     7561 tatgtcggtt tgtgagattg cgccgtagcg actgttcagc cggagcctga tcatcggcaa
     7621 cgacttttac ctgatctacc atgttttttc ttcctgtacc tgtctgtgtt gttcaggctc
     7681 tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc gatattaggt
     7741 aacaatacgc gggatgaata ctaagattta gatctaatgt taattttatg tttaaagtga
     7801 gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta cggggtcttt
     7861 gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg cctgctcccc
     7921 tctggttcga ggggaacaga atgcgctgct tagaggattt ccagcagttc gacttcaaac
     7981 accagggtgc tgaacggagg gatggatgca cctgcgccgc gctcgccata tgccagttcc
     8041 tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc ttcaatccag
     8101 ccagggatca cgccattaac cgggaattca gcgggttcac cacgagcaac ggagctgtca
     8161 aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc ggtacgtgcc
     8221 ggaattgcgc cttcaccttg gttgatcacg cggaattgca ggccagattc ggtgctattc
     8281 acaccttctt ttttggcgtt ttcttccagg tatttcacac cttcagcagc catcgcctgg
     8341 aaacgctgac gacgaacggc atcggcgcgt tcgtggattt cacgcagcgc gcgatgcacc
     8401 acatcaaccg gcacagccgg atgtttgcct tccagcgcat cggcaatacc tgcaatcagt
     8461 gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac ctgcaaacca
     8521 atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat cgtatttcct
     8581 ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg taaaactttg
     8641 tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc cgtatgaata
     8701 taataaaggc g
//
LOCUS       DADLTF010000153         8648 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.155, whole genome shotgun sequence.
ACCESSION   DADLTF010000153 DADLTF010000000
VERSION     DADLTF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.155"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..323)
                     /locus_tag="JN749_003459"
     CDS             complement(<1..323)
                     /locus_tag="JN749_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAY7369742.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(333..881)
                     /locus_tag="JN749_003460"
     CDS             complement(333..881)
                     /locus_tag="JN749_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAY7369743.1"
                     /translation="MGLPPLSKTPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYTEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(881..1588)
                     /locus_tag="JN749_003461"
     CDS             complement(881..1588)
                     /locus_tag="JN749_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7369744.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JN749_003462"
     CDS             complement(1603..2280)
                     /gene="ydjY"
                     /locus_tag="JN749_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HAY7369745.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2285..2995)
                     /locus_tag="JN749_003463"
     CDS             complement(2285..2995)
                     /locus_tag="JN749_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAY7369746.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JN749_003464"
     CDS             complement(3162..3968)
                     /gene="xthA"
                     /locus_tag="JN749_003464"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAY7369747.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4414..5634
                     /gene="astC"
                     /locus_tag="JN749_003465"
                     /pseudo
     CDS             4414..5634
                     /gene="astC"
                     /locus_tag="JN749_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5631..7364
                     /gene="astA"
                     /locus_tag="JN749_003466"
     CDS             5631..7364
                     /gene="astA"
                     /locus_tag="JN749_003466"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HAY7369748.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAAGWLAAGRETGQALSALEDLDGLLFTGSANTGYQLHRQLSGQPEKILALEMGGNNP
                     LIIDEVADIDAAVHLTIQSAFVTAGQRCTCARRLLLKSGAQGDAFLARLVAVSQRLTP
                     GNWDDEPQPFIGGLISEQAAQQVVTAWQQLEAMGGRTLLAPRLLQAGTSLLTPGIIEM
                     TGVGGVPDEEVFGPLLRVWRYDTFDEAIRMANNTRFGLFCGLVSPEREKFDQLLLEAR
                     AGIVNWNKPLTGAASTAPFGGIGASGNHRPSAWYAADYCAWPMASLESDSLTLPATLN
                     PGLDFSDEVVR"
     gene            7361..>8648
                     /gene="astB"
                     /locus_tag="JN749_003467"
     CDS             7361..>8648
                     /gene="astB"
                     /locus_tag="JN749_003467"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HAY7369749.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2021 a         2221 c         2370 g         2036 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgggaatgtc catggcagaa ttaaaacgag ccgacaggtt ttgcaggccg
      421 atcaatgccg tcagttcggt gagcgcctga gcgtcaaaat gcgcagccag tcgggtacgc
      481 agggcatcat cgactgttgg cggtgtgacg cttgcggctt cggtgtactc cagcgccagc
      541 cgttcttcat cgctaaagag cgggctttgc cgccagtcag ccactgccag cagtttatcg
      601 gtgctgccgg tacgctcggc gactttcata ctggtgatat ccacgcaaaa ctcacacagg
      661 cacatttgcg caatgcgcgc gctgacaagc gatcgcacta ccggatcgag cggtgagcgt
      721 ttgcgctcca gccacccaac aaacattgac accaaataaa agataaacgg gatccgcccc
      781 caccagcgaa tcgggcttag cacctcgccg taatggcgac gatgcagcca cgcctgcgga
      841 cgtaaaatga aaggagtttt gctaagcggc ggtaaaccca tcagtcattc ctcttctggc
      901 gctcaagcca tattttcttc gccataaaaa tcaccacggt cagggcaaac agaataaaca
      961 gttcggtgac aaaccagaaa gtaccgcctg ttaacatgct gcccgcccag gaataaacaa
     1021 tagtcgccgg taattgacca agcccggtgg cgataaaaaa cgagcgaaaa cgtattgaag
     1081 tcagaccggc agcatagctg attggatcga aagggacaaa aggcaataac cgacagacca
     1141 ggatggtgtg tttgccgtag cgagtgaaaa agccgtccat actgtcaagc acggttttgc
     1201 cggttaattt ttccaccact tcgcggccca tcactctggc gataaaaaag cacagcgccg
     1261 cgccggccat cgaactggtc cacgacagca agccgcccca gaacgcgcca aacagcgacg
     1321 cattggcaaa ggtgatcaaa aacgcaggta gcggtgcagc aatggcctgt aaaatcatca
     1381 acaagaacga gacaactgcc gccagtgcgc cgtaagactg aataaagcgt tctataccct
     1441 gttggtccac ggcggcaaac gccgcaacgc tgcgattgat aaactcatgg acgccgggaa
     1501 gcagcgccca tgcgagcagc atagcgaaca acaggatgat gagggttata cggtagtacc
     1561 agattttacg cgattgtatc ttcatcatcc tttatcctgg ctttattcgg tgattttaaa
     1621 ggtaaccgtt gccaacgtgt tatccgccgg gagaactgag gcattgcctt tgaatttcac
     1681 ttcgccgcgt ttttccaccg caccataagt gtatgttgca ttgctgacga tgccgaccgg
     1741 gcagctatcc agacacacca ggcaacccgt tttcttctct tctgctgccg ttaaattacc
     1801 gccaaagcgc atgtccagtt tcttgccatt actgtcaaca atcacttcat cgaaggaata
     1861 cgcttttgcc gccccttgcc agttgaccga aatatccagt ttgctgcctg tgacatgagt
     1921 cgtttcttta ttatccatcg tcatgttttc gcccggcgta ccacctgcct ctttcagggc
     1981 ttcataaaat gctttcgggg tcgcataagc cataaacagc gatttatgtc cgttggagcc
     2041 atctttaaac acaataccgt gacgagtgtc gtcggtgagg aaacgaccat taatttgcac
     2101 cagcatggtt accgtatggt tattggcatc gacgcgcagc ggttggctgt tcgaaagtcc
     2161 gtcgtattcc acttttgccg ccgcattctc tttttgatcg cagccgctaa gcccggcaac
     2221 agccagtaag cagagtgcag ccagtccttt tttccatgac actgaataat gttgcgacat
     2281 cgttctatcc ttcatttttt gggtgagtaa gtgggctgcg gcgcgaagca gacagatcca
     2341 catgtttgtg tcgggcgtag agttttgcga gctggatgag aataaacagc gccaggcccg
     2401 ccagacagag ttgtaaaata aagcgcagcg taatgccttc attggcgaga tcgcttgcca
     2461 tcacggtata aataacaata ccgggcaggg tcgtgagtgc cgaaataagg gtataaggcc
     2521 agaaggcgat ggtggttaat ccgtaagcgt aattttgaat attgtaagga aacaacggga
     2581 ttaagcgggt cagaataaga aaatcaatac cgttacgcgc aatgcctttt tcaatggcct
     2641 ggaaggtatg gctatggccc acgtatttca gcagtaaatc acgtcccagc cagcgcgcca
     2701 gcaggaatga gcacgaggag gccagcgttg cggcaattaa tgagagtagt gtccctaaga
     2761 gcgggccaaa aactattcct ccagcgatca ccagtatgct tcctggtaac agaaagaggg
     2821 gggcattgat gaataacaga atatagaggt tataaccgaa caatccgctc tggcgaatga
     2881 gtgtctgtaa gtggggtaaa tcggtgagca gatcgaataa accgaaagcg tggatagcgt
     2941 aaatgaccag cgcaaaaata agacaggcaa acagaaagtt acgctcagcg ttcatcgtgg
     3001 agtttcctat atcaaacttg ataatagaga agtggtcaga caactaaaaa cgcagtgacg
     3061 gtataacaaa ggacggcagg caacaaatca ttactgacag tttgttgatt tttctcaagg
     3121 ttaattctcc tgacccagtt tgagccagga gagctgctaa attagcggcg gaaggtcgcc
     3181 cagacggggg cgtgatcgga cggtttttcc atgctgcgaa tttcatagtc gatgccggtt
     3241 tctacgcaac attctgccag cggttggctg gcgagcagca ggtcgatgcg cagaccacgg
     3301 ttatcgtcaa aaccttttga gcggtaatca aaccatgaga aacgatctgc tgtttgcgga
     3361 ttagcatggc ggaaggtatc gaccaccccc cagctcatca gcctgtccat ccattcgcgc
     3421 tcttccggca ggaaagagca tttaccggta cgcagccagc gcttacggtt ttcttcgcca
     3481 atgccgatat ccagatctgt agggctgata ttcatatcgc ccataatcag taccggatta
     3541 tcacgtttga gttcggtttc caggtagttt tgcagattct gataaaactg cgcttttgcc
     3601 gggaatttta tcggatggtc gcggctttca ccctgcggga agtaaccgtt gatcacggtg
     3661 acataaccca gcagtgaggg gatttccgcc ataataatcc gccgctgcgc ctcttcgtcg
     3721 tcaccgggaa agccgcgacg cacggcaatc ggcgtctctt tggtcagcag cgccacgcca
     3781 taatggcctt tctgcccgtg ataaaacacg ttgtagccga gcttcgccac ctcttcgagc
     3841 ggaaacatat cgtcatgaac ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt
     3901 tcgacgatgg cttcaagctg gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca
     3961 aatttcatag tcgctgccat ttattcagat aaagagtgcg tggatggtag cagaatttcg
     4021 cgttgcttac cgcctgttgt taagtgattt gatcgatggt tgttaatgat gtcaacgatg
     4081 gcgcaaaaaa tgcccgcttt tggtgcgcgc tgcgtcagaa tggcgcagta atttccagta
     4141 aatttcgtca atgatcacaa ttccagaatt atattttact tctgcataac attgcgtttt
     4201 ttattctttt tattcaccca catcgcaaac gtattcactt tatatgcact ttaaatgcat
     4261 atggtttggt tataactcct tgatttccag ttagcctccg ccgtttatgc acttttatca
     4321 ctggctgaca cgaaccctgc aatctacatt tacagcgcaa acattactta ttgttaacat
     4381 acaaataacg aattatttac tgtagaggtc gctatgtctc agccaattac gcgtgaaaac
     4441 tttgatgaat ggatgatacc tgtttacgct ccggcaccct ttataccggt acgtggcgaa
     4501 ggttcgcgct tgtgggatca gcaggggaaa gagtatatcg acttcgcggg tagcattgcg
     4561 gtgaacgcgc tgggccatgc gcatccggaa ctgcgtgaag cgctgaacga acaggcgagt
     4621 aagttctggc ataccggcaa cggttacacc aacgagccgg tactgcgact ggcgaaaaaa
     4681 ttgatcgacg ccacgtttgc cgatcgcgtc ttcttttgta actccggtgc ggaagccaac
     4741 gaagcggcgc taaaactggc gcgtaaattc gctcacgacc gctacggcag ccataagagt
     4801 ggcatcgtgg cgttcaaaaa tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt
     4861 gggcagccag cctattcaca ggattttgcg ccactgccgg cggatattcg tcatgctgca
     4921 tataacgata ttaactctgc cagcgcgctg attgacgacg ccacctgtgc ggtgattgtc
     4981 gagcccatct agggcgaagg cggtgtggtg ccagccagca acgcgttttt acaaggtctg
     5041 cgtgaattgt gtgaccgcca caatgccttg ctgatttttg atgaagtaca aaccggcgtc
     5101 gggcgcactg gtgagctgta tgcctatatg cactacggcg tgacgcccga tctgttaacc
     5161 accgccaaag cgctgggcgg cggtttcccg gtcggtgcgt tgttggcaac cgaagagtgc
     5221 gcccgcgtga tgaccgttgg cactcatggc accacctatg gcggtaatcc gctggcctcg
     5281 gcggtagcag gcaaagtgct ggagctcatc aacacaccag agatgcttaa tggcgttaaa
     5341 cagcgtcacg actggtttgt tgagcgtctt aatactatta atcaccgcta tggtttgttc
     5401 agtgaagttc gcggcttagg tttgttgatt ggctgtgtac tgaatgccga ttacgccggg
     5461 caagcgaaac agatctctct ggaagcggcg aaagcaggcg tgatggtact gattgcgggt
     5521 ggcaacgtgg tgcgttttgc gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga
     5581 ctggatcgct ttgcagctgc ttgcgaacac tttgttagcc gaggttcatc atgatggtca
     5641 tccgtcccgt tgagcgctca gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg
     5701 gcggcctgac gtcgcttccc gccaatgaag ccacgctttc ggcgcgtatc gaaagggcaa
     5761 tcaaaacctg gcaaggcgaa ctgcctaaaa gcgagcaggg ctatgtgttc gtgctggaag
     5821 atagcgagac aggcaccgtg gcggggattt gcgctattga ggtggcggtt gggctgaacg
     5881 atccctggta caactatcgc gtcggcacgt tggttcacgc ctcaaaagag ctgaatgtct
     5941 ataacgcatt gccgacgctg tttcttagta acgatcacac cggcagcagc gagctgtgca
     6001 cgctgtttct cgacccggac tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc
     6061 gctttatgtt tatggcggct tttcgcgaca agtttaatga caaagtggtt gctgaaatgc
     6121 gcggggtgat tgacgaacac ggctattcac cgttctggca aagcctcggt aaacgcttct
     6181 tttcgatgga ttttagccgc gccgattttc tctgcggcac cgggcaaaag gcatttattg
     6241 cagaactgat gccgaaacat ccgatctata cccacttttt atcccaggaa gcccaggacg
     6301 tcatcggtca ggtacatccg caaaccgcgc ctgcccgcgc ggtgctggag aaagaaggtt
     6361 ttcgttaccg caactatatc gacatcttcg acggcgggcc aacgcttgag tgtgacatcg
     6421 accgcgtgcg cgccatccgt aaaagtcggc tggtggaagt ggcagcaggc tggcttgccg
     6481 ccgggcgtga aacgggtcag gcgctgagtg cgctggagga tctcgacggt ttgctgttta
     6541 ccggtagcgc caataccggc taccagctgc atcgccagct ctccggtcag ccggagaaaa
     6601 ttctcgccct tgagatgggc ggtaataatc cgctaattat cgatgaggtg gcggatatcg
     6661 acgcggctgt ccatctgacc attcagtcgg cgtttgtcac agccgggcaa cgctgcacct
     6721 gcgcccgccg tttattgctc aaaagcggag cgcagggcga tgcgtttctt gctcgtctgg
     6781 ttgccgtcag ccagcgatta acgccgggca actgggatga cgaaccgcag ccgtttattg
     6841 gcgggctgat ttctgaacag gccgcacagc aggtggttac tgcctggcag caactggaag
     6901 cgatgggcgg acgaaccctg cttgcgccgc gcttattaca agcagggaca tcgttgctga
     6961 caccagggat cattgaaatg acgggcgttg gcggtgtgcc ggatgaagag gtgttcgggc
     7021 cgcttttgcg cgtctggcgt tatgatactt ttgatgaggc gattcggatg gcgaacaaca
     7081 ctcgcttcgg actcttttgc ggtctggttt cccccgagcg ggaaaaattc gatcaactgt
     7141 tgctggaggc gcgggcgggg attgttaact ggaacaaacc gcttactggt gctgccagta
     7201 ccgcgccatt cggcggcatt ggtgcttccg gtaaccatcg ccccagcgcc tggtatgccg
     7261 cagattactg cgcatggcct atggcgagcc tggagtcgga ctcgttaaca ttgcccgcca
     7321 cgcttaaccc cgggctggat ttttccgatg aggtggtgcg atgaacgcct gggaagttaa
     7381 tttcgacggg ctggtagggc tgacgcatca ttacgcgggc ctgtcgtttg gtaatgaagc
     7441 ctctacccgt caccgttttc aggtgtctaa cccgcgactg gcggcaaaac agggcttact
     7501 gaaaatgaaa gcccttgccg atgcgggatt cccccaggcc gtgatcccgc cgcacgagcg
     7561 tccgtttatt ccggtgctgc gtcagttggg attcagtggt agcgatgagc aggtactgga
     7621 aaaagttgca cgccaggcac cgcactggct ttccagcgtc agttccgctt cgccaatgtg
     7681 ggtagccaat gcggcaacga tcgcgccatc tgccgatacg ctggatggca aagtgcatct
     7741 caccgttgcc aacctgaaca ataaatttca ccgttcgctg gaagcgcccg tcactgaatc
     7801 gctgttaaaa gcgattttta aggacgaaga gaaatttagc gtccattcgg cgttgccgca
     7861 ggtagcgttg ctcggtgatg agggggcggc aaaccacaat cgtctcggcg gtcattacgg
     7921 tgaaccgggt atgctacttt ttgtctacgg gcgagaagaa ggcaatgata cccggccttc
     7981 ccgttatccg gcgcgacaga ctcgcgaagc cagcgaggcg gtggcaaggc ttaatcaggt
     8041 gaatccccaa caggtgattt tcgcccagca aaacccggac gttatcgacc agagcgtttt
     8101 tcataatgac gtgattgccg tgagtaaccg ccaggtgctg ttttgccacc aacaggcgtt
     8161 cgctcgccag tcacagttac tggcaaacct gcgggcgcgg gtgaacggtt ttatggcgat
     8221 agaagttccg gcaactcagg tttccgtgtc ggatgcggtg tctacctatc tgtttaacag
     8281 ccaactgttg agccgcgatg atggttccat gatgttggtg ctgcctcagg agtgtcggga
     8341 acacgccgga gtatggggtt atctcaatga actccttgcc gctgacaacc cgattagcga
     8401 actaaaagtc tttgacttac gtgaaagcat ggcgaatggc ggcggcccgg cgtgcctgcg
     8461 gttgcgggtg gtattgacag aagaagaacg ccgggcggtg aatccggcgg tgatgatgaa
     8521 cgataccttg tttaatgcgc tcaatgactg ggtggatcgt tactaccgcg atcgtcttac
     8581 tgctgccgat ctggccgacc cgcaattgct gcgcgaaggg cgggaagcac tggatgtatt
     8641 gagccaat
//
LOCUS       DADLTF010000154         8496 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.156, whole genome shotgun sequence.
ACCESSION   DADLTF010000154 DADLTF010000000
VERSION     DADLTF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.156"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JN749_003468"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="JN749_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HAY7369750.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="JN749_003469"
     CDS             962..1303
                     /locus_tag="JN749_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HAY7369751.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="JN749_003470"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="JN749_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="JN749_003471"
     CDS             complement(1901..2611)
                     /locus_tag="JN749_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HAY7369752.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="JN749_003472"
     CDS             2719..3024
                     /locus_tag="JN749_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HAY7369753.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..4344
                     /locus_tag="JN749_003473"
     CDS             3223..4344
                     /locus_tag="JN749_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7369754.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYP
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTTKPAYIC"
     gene            4378..4701
                     /locus_tag="JN749_003474"
     CDS             4378..4701
                     /locus_tag="JN749_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HAY7369755.1"
                     /translation="MTARAIAMLPILTGNVGISGGNSGARESTYTITIERLPVLDNPV
                     KTSISCFSWTDAIDHGPQMTALRDGVRGKDKLDVPIKFIWNYKDLKRLLVRVTRDLSR
                     PEVLH"
     gene            complement(5293..6282)
                     /locus_tag="JN749_003475"
     CDS             complement(5293..6282)
                     /locus_tag="JN749_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369756.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA"
     gene            complement(6357..6866)
                     /locus_tag="JN749_003476"
     CDS             complement(6357..6866)
                     /locus_tag="JN749_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369757.1"
                     /translation="MSSKASALFNNNDWKLHVTDINLYENTVSLDGQSYPLSFAIKTL
                     IPGYLSGLPATSRESMELLEALAEAGVTIGNFFSNDLMTAYQRRQQNKRAEAERIAKE
                     QRIQAERMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADIE
                     SGGNWMDSL"
     gene            complement(6968..7189)
                     /locus_tag="JN749_003477"
     CDS             complement(6968..7189)
                     /locus_tag="JN749_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369758.1"
                     /translation="MTTKQHIDPRTPIGKATLRYRGLPTRRLLSMLGMGVEDPERPFY
                     SRDELIDLLVDRDLNNQLRRAFAKLDATH"
     gene            complement(7436..8281)
                     /locus_tag="JN749_003478"
     CDS             complement(7436..8281)
                     /locus_tag="JN749_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369759.1"
                     /translation="MNTNEVSVINLDQLVSMSSYDFLTKVINPARVEAGEKPVRNNDF
                     VSRIEDELDGQNVAYEIFVSFGQDVKSYTLNTDQLLLVGMRESKAVRRKVLAYIRQKE
                     QEVLELQAQQLQVAQTKAICYAPGAIVLDSKLDSKSGATPTEVIKALDLKLTSSKMLT
                     ASVLTGYLKTLCRPNPFYTKRVTEFGTTDIDLSTGRPRAIFNKKGETNGYYRLQPQPN
                     DKEPTIKILRKGYDWLKDNWKDIAHDMRTSNIKVVRRLVDSHLNGKATKSQKAAAGKG
                     SDYGF"
BASE COUNT         2126 a         2133 c         2130 g         2107 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agctgtatat attcagtacc cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gcccccggtg gcctctttgt caggactcag
     4801 ggtggcaccg gggaacattg gtgtcttaat tgtattgaat gaggtctcta acttttggtc
     4861 caaaactcat cgttagctgc tcgccagctc acagccgaac ccgaactgta agcacgacca
     4921 ccagcaccag cttcagcagg aagacgacaa gatagacttt atgcaggtcc atgcgtcctc
     4981 ctgtgtctat tgtaccactg cactagataa ctagtacaaa ataaagtcca gaccgaaggc
     5041 ccagaccata gttaattaat cacattgata atccaccacc tgaagtcctg ccaacattca
     5101 gtccagcctt acccatgcta ggagtcttaa ccggggtgtc gttctgtgtc tccaccactg
     5161 gtgccgcatc aggctctaca ggaagctcca cagcaggcgc agggaccgga ggtgatacgg
     5221 atgtaccacc accagcggag aagcccttag cgatggaccg gaggccttta ctaatgctct
     5281 tacccattgc tgttacgctt cggtcttggt gaacttcagg cgtttgacta caggcaggac
     5341 tgcggttgca gcgccagtgt ccagcttaac tgtcgtagca gcttcagcga caacgaagac
     5401 cttaccgagg atttccaagt cgtgggactg agagtcgcca tcacgtgaaa ggacgaccgt
     5461 aatattctca ccgtcgtgac ggaagcagcc gaagctcagg gccgtgaagc gtttagccag
     5521 catgaacaca cctttgtcct caccgttgta atacgcgatg tcggtaccca gcatatccac
     5581 gatagcgtct tcaccagaga agccctgttt agcagccaga cgggacagcg ctttcagttg
     5641 ggactcatgg gcgatgatgc cgtaatcaga cacttcagag ccgaaagagg actcggtgtt
     5701 ctcaaacaga gcgtccatga tagcgtctgc cttagtggtc aggcttccag tcaggactgg
     5761 cagctctacg gtcttcagca gcttggagga ctccagaacg tcaaccatgc gagcgttgat
     5821 ggtacggacc acattctcac agatgttcat agcgtcctgc tggagtgtgc catgagtgaa
     5881 accagtggag gacttggcgt cccacagttt agtttgctcc gagatgagga ttgcctgttc
     5941 gataggttcg acgacaatct cctgaccaga cggaacggtc acgacttcag gtcgctcacc
     6001 accgcgtaca gggacgacca cagagcggtc agacagggtg tgggcgatga ttggggtagg
     6061 caggctattg cgggtaggca ggacttctac agggaacgga atgttatcct gagcgtcttt
     6121 aagtacaccg ttcagcatag ccttaaccgt cttagctaac tggagggcat agacctgtgg
     6181 gtcagcgtta gttggcagac cttcgttaac ctgaatgttg tcggtgtcgg tgatacgtac
     6241 tggctcaatg ccgaactgat agctatcttt aatcatggtc ataataatca atctcctaaa
     6301 ttagttttag atactgtggg tcccgacatc aggtcaggac cacttgtgtg tctgtcttag
     6361 aggctgtcca tccagttacc gcctgactca atgtcagcga caatagcggc acgggagcga
     6421 ccagcggaat gggtagcact gcggaggttc tcaccatagg tccggcgctc agctttgacc
     6481 tgttcgcggc gctgtagctc tttctgccac tctgcatcgg tcatgttctc ttcacgcata
     6541 cgctcagcct gaatgcgctg ctctttagcg atacgctcag cctcggctcg cttgttctgc
     6601 tggcgacgtt ggtaggcggt catcaggtca ttgctgaaga agtttccaat ggtcacgcca
     6661 gcttcagcca gagcttccag taattccatg gactcgcggc tggtggcagg cagtcctgac
     6721 aggtagcccg gaatcagggt cttaatagcg aaggacagtg ggtaggactg accgtccaga
     6781 ctcacagtgt tttcgtagag attgatgtcg gtgacatgga gtttccagtc attgttgttg
     6841 aataacgcac tagctttgct agacatagta attactcctc ttgagagttg agactatcgg
     6901 taccgcagac acagttatgc ctacgggtct ccccgcatcg gtcatctcaa aaggtttaaa
     6961 ttaggggtta gtgagtggcg tccagcttag cgaacgctcg gcgtaactga ttgttcaggt
     7021 cacggtcgac cagcaggtct atcagttcgt ctcgactata gaaaggacgc tcagggtcct
     7081 ctacgcccat ccccagcatt gacaggaggc gacgggtagg cagaccacgg taacggagag
     7141 tagcctttcc gatgggtgtc cgtgggtcga tatgttgttt agttgtcatt gttagaagtc
     7201 ctcgttagac caatcatcca cgtcaaattc actggtgata ggagcttgga agttaggtag
     7261 accctctggt atctctagag gcagtcgctc gtcgactctc agtgccatct cttcgtgtat
     7321 atgccgaagc agagaagtca gtaagatagg gtccgcagga tgctcagggc cgtgtctctc
     7381 aatgaaacat atggttgtcg ctattgactc cataatcagt tcatcagtga gtcgcttaga
     7441 acccatagtc agaccccttg cccgctgctg ctttctggga ctttgtagcc ttaccattca
     7501 ggtgagaatc caccagacga cggacaacct tgatattgga tgtgcgcatg tcatgagcga
     7561 tatccttcca gttatccttc agccagtcat aacccttgcg tagaatcttg atggtaggct
     7621 ctttgtcgtt aggctgtggc tgtagacgat agtagccgtt ggtctcacct ttcttattga
     7681 agatggctcg tggacgtccg gtcgataggt ctatatccgt agtgccaaac tcagtaacac
     7741 gcttggtgta gaatgggtta ggacgacaca gggtcttcag atatccagtc aggacgctag
     7801 ctgtcagcat cttagagctg gtcaacttaa ggtccagagc tttaataacc tcggtaggcg
     7861 tggcgcctga cttggagtcc agcttggagt cgaggacgat agcaccgggc gcatagcaga
     7921 tagctttggt ctgtgcgacc tgaagttgct gggcctgaag ttcgaggacc tcttgctcct
     7981 tctggcgaat ataggctagg accttacgac gcacagcttt agactcacgc atcccgacca
     8041 gcagaagctg gtcagtgttg agggtatagc ttttaacgtc ctgcccgaag cttacgaaaa
     8101 tttcgtaggc tacattttga ccgtccagct catcttcaat acgggacaca aagtcgttgt
     8161 tacggacagg cttctcccca gcctccacac gagccgggtt gatgacctta gtcaagaaat
     8221 cgtagctact catagacaca agctggtcaa ggttgatgac gctaacttcg ttggtattca
     8281 taaaggatgc tcctttggta aagcactttc tgtctccatg tggagtccgc acctattcaa
     8341 tgcatccaaa cggacccacc aaaaagtgct taacgaaagt gaatagggtt aatgttgatt
     8401 agtgttggtc tggagtgacc atgttctcca tgtgcatata aggcgagacc gcatgtcagg
     8461 acgagctgta aggaccccag atggtgagcg gtgata
//
LOCUS       DADLTF010000155         8375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.157, whole genome shotgun sequence.
ACCESSION   DADLTF010000155 DADLTF010000000
VERSION     DADLTF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.157"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(58..555)
                     /locus_tag="JN749_003479"
     CDS             complement(58..555)
                     /locus_tag="JN749_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7369760.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTIQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(731..1344)
                     /locus_tag="JN749_003480"
                     /pseudo
     CDS             complement(731..1344)
                     /locus_tag="JN749_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012599877.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            1636..2376
                     /locus_tag="JN749_003481"
     CDS             1636..2376
                     /locus_tag="JN749_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HAY7369761.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(2347..3114)
                     /locus_tag="JN749_003482"
     CDS             complement(2347..3114)
                     /locus_tag="JN749_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HAY7369762.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(3320..3898)
                     /gene="lpcA"
                     /locus_tag="JN749_003483"
     CDS             complement(3320..3898)
                     /gene="lpcA"
                     /locus_tag="JN749_003483"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HAY7369763.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            4138..6582
                     /locus_tag="JN749_003484"
     CDS             4138..6582
                     /locus_tag="JN749_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HAY7369764.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDTMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(6625..7098)
                     /gene="ivy"
                     /locus_tag="JN749_003485"
     CDS             complement(6625..7098)
                     /gene="ivy"
                     /locus_tag="JN749_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HAY7369765.1"
                     /translation="MGRISSGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSAIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            7252..8022
                     /gene="yafV"
                     /locus_tag="JN749_003486"
     CDS             7252..8022
                     /gene="yafV"
                     /locus_tag="JN749_003486"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HAY7369766.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            8131..8363
                     /locus_tag="JN749_003487"
                     /pseudo
     CDS             8131..8363
                     /locus_tag="JN749_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
BASE COUNT         2027 a         2108 c         2234 g         2006 t
ORIGIN      
        1 gttaaagcac gatttgtagg ccggataagc gtaagcgcat ccggcaccag cgcacagtta
       61 taaaatgata cgctccccgg cacttaaatc cgttatgtct cccgcccaat cgatgggata
      121 taaccctcgc gcgacatcgc gatggaacgt tgagaatggc caatcactca cctgctttac
      181 ccagccatgt ttctctggat ttatataaat ataatcaaca tgatggcgat aatcttttgt
      241 attgcgaatg gcatgctccc aaaaacgcgg ttgccagata tttttcaatc cgcaagcatg
      301 ggtgaactgc tttttaatct cccgccagcg agaggaaaaa tcatcatcgc tttcgggtaa
      361 tgtccatatg caatgcatat gctccggcaa aacgacccag gcgttaattt caaaaggtct
      421 gtctcgctta acgttaataa tggcgttacg gagcgtctgg aactggatgg tcagaagatg
      481 gcttcgacga ttgcgtaaat ttaccgtgaa aaaccatgtt ccccccttga tgtaataacg
      541 acgatattcc gacatagcga ctcctttttc tcaacggtaa gaggaaaagg aaaggccgac
      601 aatgacgctg tgttatatcg ggaaagcggc tcgcaaaaac gcacagattt gtaggcatga
      661 taagacgcgt cagcgtcgca tcaggcatcc gtgcacaacc gccggatgcg gcgtaaacgc
      721 cttatccggc ctacaaaatc atctcttccg tcaaaatcct gcgtgcgcct aaaaaatggt
      781 cctgccagaa aggttcggct aaccggctta cccgaatggt ttccccggtg cgcagtgact
      841 caataaactg accgtcgccc agatacaccc ccatatggtc ggcaatctcg cggctgtgga
      901 tatggaaaaa tagcaaatct ccccggcgca ggtcgttatt cgccacaatc gttgcccgac
      961 ggtagtggta catctcatta gccgtgcgcg ggagtttcgc ctcaaggatc ttgttgtagg
     1021 cataaaaaac taacccacta caatcgaagc cttgatcggg ccgcgtgccg ccccagacgt
     1081 aaggcttgcc aagctgctgc tccaggcggt gaatcgccac ttcggtgatg ttatgcaggt
     1141 gatcgctgct taaaaagtgg ttgttttccg ccagctcctg ccagcgtctg tcactcgcct
     1201 tgagcggcgc aggaaaccat tctggatgct gttttatctg ctcccggttg tggtggcgct
     1261 ggcgagtaaa gggagcaaaa cgcataacga aaacacgcct ggatgcaaaa atctgccgag
     1321 caagaacgaa gacatgaaag acataaaccg gctgaatgaa ggtgagaatt ttaaaagacc
     1381 ggaggagtaa acgggattat taattgctta tttgcatatt actattgaga tttcataata
     1441 ttaggaaata aatgatacat gcgcttttcg gactattgaa aactgcccct gtgctaaatt
     1501 tgtaaaccca gcgccgacag gcttgcgtaa aactcttttg gtgataatgt gagccttcca
     1561 aatccagact attccgattt gggagccatt ttgccgtcct ggcgtgtgta accgttttat
     1621 caaggaataa gcagtatgcg taaaatcgca ttaattcttg cgatgttgtt gatcccgtgc
     1681 gtttcgtttg ccggtttgct gggcagcagt agctcgacca cgccagtgag caaagagtat
     1741 aagcagcagt tgatgggatc ccctgtctac atccagatct tcaaggaaga acgtacgctc
     1801 gatctttacg tcaaaatggg cgagcaatat caactgctcg acagttataa aatctgtaaa
     1861 tattccggcg gcttagggcc aaaacagcgt cagggcgatt tcaaaagccc ggaagggttt
     1921 tatagcgtcc agcgtaatca gttaaaacca gacagccgtt actacaaagc gattaatatt
     1981 ggattcccca atgcttatga ccgtgcgcat ggttatgaag ggaaatacct aatgatccac
     2041 ggcgattgtg tatccatcgg ctgctacgca atgaccaatc agggtattga tgagatcttc
     2101 cagttcgtta ctggtgcgct ggtgtttggt cagccgagcg tgcaggtgag tatttaccca
     2161 ttccgcatga ccgacgccaa tatgaagcgc cataaatatt ccaactttaa ggacttctgg
     2221 gagcagctga agccgggcta cgactacttt gagcaaaccc gtaagccacc gaccgtttct
     2281 gtggccaatg gccgttacgt ggtcagcaag ccgttaagcc acgaagtggt gcagccacaa
     2341 ctggcatcaa actacacgct ccccgaggca aaataagcgc cattcgcctg gcataatttt
     2401 ttgccaggtt tcattgcccg tcagcggctg tgtcgcaatc accgtgacca catcattcgg
     2461 tgtggtctgc gagctgaagt cgatttccac atcctgatcc agcaacgttg ccacgccaaa
     2521 cggcgcgcgg cgggtgatcc agtgtaaatt agtcgagcaa tacgccatta catagcgccc
     2581 gtccgaaagc agcatgttga aaacgccctt cgcccgcagt tcatccgcca gtgaggcgat
     2641 atatttaaat actgccgcca tgttgcccgg cgtgcgcggg taacgctgcg ttaatttatg
     2701 cagcagccag caaaaggctt tttcgctgtc ggtttcaccg accgggcgga agttgccggt
     2761 ttccagtgat ttgtagcccg tcagttgtcc gttatgggca taagtccagt tacgccccca
     2821 taactcgcgg gtaaatgggt gagtattttc cagcgccacc tcgccccgat tagcctggcg
     2881 aatatgagcc accaccgaac aggattttat cgggtagtcc tggacaagtt tggcgatggg
     2941 ggaattaaag ctgggttgtg gatctttaaa tgtgcgacag cctttacctt cgtaaaaggt
     3001 aatgccccag ccatctttat gtggcccggt tccaccacca cgctgtacaa gtccggtgaa
     3061 actaaagcag atatcggtag ggacgttggc gctcatcccg agcaattcgc acatgggtaa
     3121 cctccacaac agcgggagcg catggacccc ggcgaagatt gatttaagaa ttttcaggtc
     3181 ggatgcggcg taaacgaatt attcggccta cggttcgggt acagatttgt aggcatgata
     3241 agacgcgtca gcatcgcatc aggcatcagc gcacaagtgc cggatgcggc gtaaacgcct
     3301 tatccggcct acgccagact tacttaacca tctctttttc aattaactgg atcaggatat
     3361 ggatcacttt aatgtgaatc tcctgaatgc ggtcggcata accaaagtgc ggaacgcgaa
     3421 tttcgatatc cgccgtgcca gccattttac cgccgtcttt accggtcagg gtgatcactt
     3481 tcatcccctt ctcacgcgcc gctgcgatcg ctttgatcac gtttgcagag ttaccggagg
     3541 tggagatccc cagcagtaca tcgccttcgc gacctaccgc ttcaacgtag cgggagaaaa
     3601 tatcattgaa accgaaatca ttaccgacgc aggaaatatg actaacgtca gaaatagcaa
     3661 tcgccgggta gcccggacgg ttttcacggt agcgaccggt caactcttcg gcaaagtgca
     3721 tagcgtcgca atgggaaccg ccgttgccgc aggaaagcac tttgccaccg gctttaaagc
     3781 tgtctgctaa caggaccgcc gcgcgctgaa tggcgtgaat attggcgtca tcttttaaaa
     3841 agttagccag cgtttccgcc gcttcgttca gttcgttacg aataagatcc tggtacatga
     3901 ggatatcctt cagcataaat gtaatagaca aaatgcagtg taccggatac cgccaaaagc
     3961 gagaagtacg ggcaggtgct atgaccagga ctttttgacc tgaagtgcgg ataaaaacag
     4021 caacaatgtg agctttgttg taattatatt gtaaacacat tgctaaatgt ttttacatcc
     4081 actacaacca tatcatcaca agtggtcaga cctcctacaa gtaaggggct tttcgttatg
     4141 atgattttga gtattctcgc tacggttgtc ctgctcggcg cgttgttcta tcaccgcgtg
     4201 agcttattta tcagcagtct gattttgctc gcctggacag ccgccctcgg cgttgctggt
     4261 ctgtggtcgg cgtgggtact ggtgcctctg accattatcc tcgtgccatt taactttgcg
     4321 cctatgcgta agtcgatgat ttccgcgccg gtatttcgcg gtttccgtaa ggtgatgccg
     4381 ccgatgtcgc gcactgagaa agaagcgatt gatgcgggca ccacctggtg ggagggcgac
     4441 ttgttccagg gcaagccgga ctggaaaaag ctgcataact atccgcagcc gcgcctgacc
     4501 gctgaagagc aagcgtttct cgacggcccg gtagaagaag cctgccggat ggcgaatgat
     4561 ttccagatta ctcatgagct ggcggatctg ccgccggagt tgtgggcgta ccttaaagag
     4621 catcgcttct tcgcgatgat tatcaaaaaa gagtacggcg gactggagtt ctcagcttat
     4681 gcccagtctc gtgtgctgca aaaactctct ggcgtgagcg ggatcctggc gattaccgtc
     4741 ggcgtgccaa actcattagg cccgggcgaa ctgttgcaac attacggcac tgacgagcag
     4801 aaagatcact atctgccgcg tctggcgcgt ggtcaggaga tcccctgctt tgcactgacc
     4861 agcccggaag cgggttccga tgcgggcgcg attccggaca ccgggattgt ctgcatgggc
     4921 gaatggcagg gccagcaggt gctggggatg cgtctgacct ggaacaaacg ctacattacg
     4981 ctggcaccga ttgcgaccgt gcttgggctg gcgtttaaac tctccgaccc ggaaaaatta
     5041 ctcggcggtg cagaagattt aggcattacc tgtgcgctga tcccaaccac cacgccgggc
     5101 gtggaaattg gtcgtcgcca cttcccgctg aacgtaccgt tccagaacgg accgacgcgc
     5161 ggtaaagatg tcttcgtgcc gatcgattac ataatcggcg ggccgaaaat ggccgggcaa
     5221 ggctggcgga tgctggtgga gtgcctctcg gtaggccgcg gcatcaccct gccttccaac
     5281 tcaaccggcg gcgtgaaatc ggtagcgctg gcaacgggcg cgtattctca cattcgccgt
     5341 cagttcaaaa tctctatcgg taagatggaa gggattgaag agccgctggc gcgtattgcc
     5401 gggaacgctt atgtaatgga tgctgcggca tcgctgatta cctacggcat tatgctcggc
     5461 gaaaaacctg ccgtgctgtc ggctatcgtt aagtatcact gtacccaccg cgggcagcag
     5521 tcgattattg atacgatgga tattaccggc ggtaaaggca ttatgctcgg gcaaagcaac
     5581 ttcctggcgc gtgcttacca gggcgcaccg attgccatca ccgttgaagg ggctaacatt
     5641 ctgacccgca gcatgatgat cttcggacaa ggagcgattc gttgccatcc gtacgtgctg
     5701 gaagagatgg aagcggcgaa gaacaatgac gtcaacgcgt tcgataaact gttgttcaaa
     5761 catatcggtc acgtcggtag caacaaagtt cgcagcttct ggctgggcct gacgcgcggt
     5821 ttaaccagca gcacgccaac cggcgatgcc actaaacgct actatcagca cctgaaccgc
     5881 ctgagcgcca acctcgccct gctttctgat gtctcgatgg cactgctggg cggcagcctg
     5941 aaacgtcgtg agcgtatctc ggcacgtctg ggggatattt taagccagct ttacctcgcc
     6001 tctgccgttc tgaagcgtta tgacgacgaa ggccgtaatg aagccgatct gccgctggtg
     6061 cactggggcg tacaggatgc gctgtatcag gcagaacagg cgatggacga tttactgcaa
     6121 aacttcccga accgcgtggt tgccgggctg ctgaatgtgg tgatcttccc gaccggacgt
     6181 cattatctgg caccgtctga caagctggat cataaagtgg cgaagatttt acaagtgccg
     6241 aacgccactc gttcacgtat tggtcgcggt cagtacctga cgccaagcga gcataacccg
     6301 gttggtttac tggaagaggc gttggtggat gtgattgccg ccgactcgat tcatcagcgg
     6361 atctgtaaag agctgggtaa aaatctgcca tttacccgtc tggatgaact ggcgcacaac
     6421 gcgctgacga aggggctgat tgataaagat gaagccgcta ttctggtgaa agcggaagaa
     6481 agccgtctgc gcagtattaa cgttgatgac tttgatccgg aagagctggc gacgaagccg
     6541 gtaaagttgc cggagaaagt gcggaaagtt gaagccgcgt aattgaataa cggagccgaa
     6601 aggctccgtt tctttatccg ctaattattt aaaattaaag ccatccggat ggttttccag
     6661 gctgccggtc aacgccgcga acagcaccgt tttaccatca atcgaaagcg catcgttcac
     6721 attcagccag gtgagtttct cttgcgacgt tttctcatca atagccgaga acagccccgt
     6781 catctgatta gatttctcgg accacatcac agcgatacgt tgcgagccac agtcatgcgg
     6841 tttgcacgcg ctcatcacct gatacgtctc atctcccaac gttacggttt gtgcgggagt
     6901 ataagtaccg cctttcatca cccatgcagg tagcttatgc ccttgcacca tctgattaaa
     6961 cgcagctttg gtggtttcgc cctttgcaag gctgctaatg gttaaatcat cctgcgccat
     7021 tgcactggtg gcgatgacca gtgcggcgac tgtcgttatt gccttaaaca tcattcctcc
     7081 cgagcttatc ctgcccatgt tattaacctc ctaaagcctg gcgataacac gataatttgc
     7141 aaatcagata tttccaatat cagcatttgt tatcaattta ttctgcgcta aggcaactgc
     7201 ctgataaggt gttaaagtta taaaaccctt cgcataaagg agttgtacac cgtgcctagt
     7261 ttgaagatta cgcttttgca gcaaccgttg gtgtgggtgg atggtcctgc caacctgcgc
     7321 cattttgatc gtcaactgga aggtattacc gggcgcgatg tgatcgttct accggagatg
     7381 tttaccagcg gctttgccat ggaagcggca gcttcgtcgc ttgcacaaga tgacgtagtg
     7441 aactggatga cagctaaggc gcagcagtgc aatgcgctga ttgcaggcag tgttgcatta
     7501 caaacggagt ctggttcggt taaccgcttt ttgctggttg agccgggcgg cacggtacat
     7561 ttttatgata agcgtcatct gttccgcatg gcagatgagc atctgcatta taaagcgggc
     7621 aatgcgcgag tgattgtgga atggcgcggc tggcgtattt tgccgctggt gtgctacgac
     7681 ttacgttttc cagtgtggtc gcgcaatctc aacgattatg acctcgccct gtacgtcgcc
     7741 aactggccag ctccgcgctc tctgcactgg caggcattgc tgacggcccg cgcgattgag
     7801 aatcaggcgt atgtggcggg atgcaatcgc gtcggcagcg atggcaacgg ctgccattat
     7861 cgcggtgaca gccgggtgat taatccgcaa ggcgagatta tcgctaccgc cgacgcgcat
     7921 aaggcaacgc gtattgatgc ggagctgtcg atggtggcat tgcgggaata tagagaaaag
     7981 tttccggcat ggcgggatgc ggatgagttt aggttgtggt gagtgggggt tgtgtttagg
     8041 gccggggctg ggctagatta cgaaagcccc taacttaaac acaatattac gccagaagtc
     8101 accatttcca aaacacagcg tcgctgtacc gtgggttatc tgcccaaccg cagcgacacg
     8161 ccggtgatca aaatcagcgc caaatggctg agaaaagcgg gatttgagat cggcacgggc
     8221 gttaccgtga agatctcgga gggatgtttg atcctgcttg ggataacaac gaagtacagg
     8281 aactgcgcag ggaactttat caggttaaac agtcagttaa ggggatgcga gacgggatgt
     8341 ttagtgtgct gaacgaaagt taaataataa aaagc
//
LOCUS       DADLTF010000156         8344 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.158, whole genome shotgun sequence.
ACCESSION   DADLTF010000156 DADLTF010000000
VERSION     DADLTF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.158"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JN749_003488"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="JN749_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7369767.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(964..1437)
                     /locus_tag="JN749_003489"
     CDS             complement(964..1437)
                     /locus_tag="JN749_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY7369768.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1597..2610
                     /gene="intI1"
                     /locus_tag="JN749_003490"
     CDS             1597..2610
                     /gene="intI1"
                     /locus_tag="JN749_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7369769.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2813..3163
                     /locus_tag="JN749_003491"
     CDS             2813..3163
                     /locus_tag="JN749_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY7369770.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3289..3849
                     /locus_tag="JN749_003492"
     CDS             3289..3849
                     /locus_tag="JN749_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7369771.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3852..6818
                     /locus_tag="JN749_003493"
     CDS             3852..6818
                     /locus_tag="JN749_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HAY7369772.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6885..7262
                     /locus_tag="JN749_003494"
     CDS             6885..7262
                     /locus_tag="JN749_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7369773.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7463..8122)
                     /locus_tag="JN749_003495"
     CDS             complement(7463..8122)
                     /locus_tag="JN749_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7369774.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1841 a         2434 c         2270 g         1799 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc gcttccctca tgatgtttaa cgcctggcac agcggatcgc
      901 aaacctggcg cggcttttgg tacaaaaggc gtgacaggtt tgcgaatccg ttgctgccac
      961 ttgttaaccc ttttgccaga tttggtaact ataatttatg ttagaggcga agtcttgggt
     1021 aaaaactggc ctaaaattgc tggggatttc aggaaagtaa acatcacctt ccggctcgat
     1081 gtctattgta gatatatgta gtgtatctac ttgatcgatc aggcttttgt atatctcccc
     1141 accacctgaa acaatgacat gatccgttat tttctttagg ttggttaaag catctttaat
     1201 tgatggaaag atcactacgt tctcattgtc agatgtaaaa cttgaacgtg ttacgaccgc
     1261 atactttcgg ttgggtaatg ctcccattga ttcaaaagtc ttgcgtccaa ccaacagcca
     1321 ttggttatag gtaatagctt taaacaggag ctgttcacct ttggcactcc atggaatatc
     1381 agggccattc ccgataactc cattcttcga tatagctacc attagtgata gtttcacaat
     1441 tcttcctcag aggttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     1501 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     1561 atagactgta ccccaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     1621 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     1681 tacagcttac gaaccgaaca ggcttatgtc cactgggttc gtgccttcat ccgtttccac
     1741 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     1801 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     1861 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     1921 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     1981 ctggaaggcg agcatcgttt gttcgcccag cttctgtatg gaacgggcat gcggatcagt
     2041 gagggtttgc aactgcgggt caaggatctg gatttcgatc acggcacgat catcgtgcgg
     2101 gagggcaagg gctccaagga tcgggccttg atgttacccg agagcttggc acccagcctg
     2161 cgcgagcagc tgtcgcgtgc acgggcatgg tggctgaagg accaggccga gggccgcagc
     2221 ggcgttgcgc ttcccgacgc ccttgagcgg aagtatccgc gcgccgggca ttcctggccg
     2281 tggttctggg tttttgcgca gcacacgcat tcgaccgatc cacggagcgg tgtcgtgcgt
     2341 cgccatcaca tgtatgacca gacctttcag cgcgccttca aacgtgccgt agaacaagca
     2401 ggcatcacga agcccgccac accgcacacc ctccgccact cgttcgcgac ggccttgctc
     2461 cgcagcggtt acgacattcg aaccgtgcag gatctgctcg gccattccga cgtctctacg
     2521 acgatgattt acacgcatgt gctgaaagtt ggcggtgccg gagtgcgctc accgcttgat
     2581 gcgctgccgc ccctcactag tgagaggtag ggcagcgcaa gtcaatcctg gcggattcac
     2641 tacccctgcg cgaaggccat cggtgccgca tcgaacggcc ggttgcggaa agtcctccct
     2701 gcgtccgctg atggccggca gcagcccgtc gttgcctgat ggatccaacc cctccgctgc
     2761 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga caatggaggt
     2821 ggtagccgag ggtgtggaaa cacccgactg ccttgcgtgg ttgcggcagg cgggttgcga
     2881 cacggtgcag ggtttcctgt tcgccaggcc gatgccggcg gcggccttcg tcggcttcgt
     2941 caaccaatgg aggaacacca ccatgaacgc caatgaaccg agcaccagtt gctgcgtgtg
     3001 ctgcaaggaa atcccgctcg atgccgcctt cacgccggaa ggggccgagt acgtggagca
     3061 tttctgcggg ctggagtgct atcagcgctt ccaggcgcgg gccagcactg cgaccgaaac
     3121 cagcgtcaaa ccggacgctt gtgattcgcc gccgtcaggt tgaggcatac cctaacctga
     3181 tgtcagatgc catgtgtaaa ttgcgtcagg ataggattga attttgaatt tattgacata
     3241 tctcgttgaa ggtcatagag tcttccctga cattttgcag ggaattccat gactggacag
     3301 cgcattgggt atatcagggt cagcaccttc gaccagaacc cggaacggca actggaaggc
     3361 gtcaaggttg atcgcgcttt tagcgacaag gcatccggca aggatgtcaa gcgtccgcaa
     3421 ctggaagcgc tgataagctt cgcccgcacc ggcgacaccg tggtggtgca tagcatggat
     3481 cgcctggcgc gcaatctcga tgatttgcgc cggatcgtgc aaacgctgac acaacgcggc
     3541 gtgcatatcg aattcgtcaa ggaacacctc agttttactg gcgaagactc tccgatggcg
     3601 aacctgatgc tctcggtgat gggcgcgttc gccgagttcg agcgcgccct gatccgcgag
     3661 cgtcagcgcg agggtattgc gctcgccaag caacgcgggg cttaccgtgg caggaagaaa
     3721 tccctgtcgt ctgagcgtat tgccgaactg cgccaacgtg tcgaggctgg cgagcaaaag
     3781 accaagcttg ctcgtgaatt cggaatcagt cgcgaaaccc tgtatcaata cttgagaacg
     3841 gatcagtaaa tatgccacgt cgttccatcc tgtccgccgc cgagcgggaa agcctgctgg
     3901 cgttgccgga ctccaaggac gacctgatcc gacattacac attcaacgat accgacctct
     3961 cgatcatccg acagcggcgc gggccagcca atcggctggg cttcgcggtg cagctctgtt
     4021 acctgcgctt tcccggcgtc atcctgggcg tcgatgaact accgttcccg cccttgttga
     4081 agctggtcgc cgaccagctc aaggtcggcg tcgaaagctg gaacgagtac ggccagcggg
     4141 agcagacccg gcgcgagcac ctgagcgagc tgcaaaccgt gttcggtttc cggcccttca
     4201 ccatgagcca ttaccggcag gccgtccaga tgctgaccga gctggcgatg caaaccgaca
     4261 aaggcatcgt gctggccagc gccttgatcg ggcacctgcg gcggcagtcg gtcattctgc
     4321 ccgccctcaa cgccgtcgag cgggcgagtg ccgaggcgat cacccgtgct aaccggcgca
     4381 tctacgacgc cttggccgaa ccactggcgg acgcgcatcg ccgccgcctc gacgatctgc
     4441 tcaagcgccg ggacaacggc aagacgacct ggttggcttg gttgcgccag tctccggcca
     4501 agccaaattc gcggcatatg ctggaacaca tcgaacgcct caaggcatgg caggcactcg
     4561 atctgcctac cggcatcgag cggctggttc accagaaccg cctgctcaag attgcccgcg
     4621 agggcggcca gatgacaccc gccgacctgg ccaaattcga gccgcaacgg cgctacgcca
     4681 ctctcgtggc gctggccacc gagggcatgg ccaccgtcac cgacgaaatc atcgacctgc
     4741 acgaccgcat cctgggtaag ctgtttaacg ctgccaagaa taagcatcag cagcagttcc
     4801 aggcgtcagg caaggccatc aacgccaagg tacgtctgta cgggcgcatc ggtcaggcgc
     4861 tgatcgacgc caagcaatca ggccgcgatg cgtttgccgc catcgaggcc gtcatgtcct
     4921 gggattcctt tgccgagagc gtcaccgagg cgcagaagct cgcgcaaccc gatgacttcg
     4981 atttcctgca tcgcatcggc gagagctacg ccaccctgcg ccgctatgca ccggaattcc
     5041 ttgccgtgct caagctgcgg gccgcgcccg ccgccaaaaa cgtgcttgat gccattgagg
     5101 tgctgcgcgg catgaacacc gacaacgccc gcaagctgcc agccgatgca ccgaccggct
     5161 tcatcaagcc gcgctggcag aaactggtga tgaccgacgc cggcatcgac cggcgctact
     5221 acgaactgtg cgcgctgtcc gagttgaaga actccctgcg ctcgggcgac atctgggtgc
     5281 agggttcacg ccagttcaag gacttcgagg actacctggt accgcccgag aagttcacca
     5341 gcctcaagca gtccagcgaa ttgccgctgg ccgtggccac cgactgcgaa caatatctgc
     5401 atgagcggct gacgctgctg gaagcacaac ttgccaccgt caaccgcatg gcggcagcca
     5461 acgacctgcc ggatgccatc atcaccgagt cgggcttgaa gatcacgccg ctggatgcgg
     5521 cggtgcccga caccgcgcag gcgctgatag accagacagc catggtcctg ccgcacgtca
     5581 agatcaccga actgctgctc gaagtcgatg agtggacggg cttcacccgg cacttcacgc
     5641 acttgaaatc gggcgatctg gccaaggaca agaacctgtt gttgaccacg atcctggccg
     5701 acgcgatcaa cctgggcctg accaagatgg ccgagtcctg ccccggcacg acctacgcga
     5761 agctcgcttg gctgcaagcc tggcataccc gcgacgaaac gtactcgaca gcgttggctg
     5821 aactggtcaa cgctcagttt cggcatccct ttgccgggca ctggggcgat ggcaccacat
     5881 catcatcgga cggacagaat ttccgaaccg ctagcaaggc aaagagcacg gggcacatca
     5941 acccaaaata tggcagcagc ccaggacgga ctttctacac ccacatctcc gaccaatacg
     6001 cgccattcca caccaaggtg gtcaatgtcg gcctgcgcga ctcaacctac gtgctcgacg
     6061 gcctgctgta ccacgaatcc gacctgcgga tcgaggagca ctacaccgac acggcgggct
     6121 tcaccgatca cgtcttcgcc ctgatgcacc tcttgggctt ccgcttcgcg ccgcgcatcc
     6181 gcgacctggg cgacaccaag ctctacatcc cgaagggcga tgccgcctat gacgcgctca
     6241 agccgatgat cggcggcacg ctcaacatca agcacgtccg cgcccattgg gacgaaatcc
     6301 tgcggctggc cacctcgatc aagcagggca cggtgacggc ctcgctgatg ctcaggaaac
     6361 tcggcagcta cccgcgccag aacggcttgg ccgtcgcgct gcgcgagttg ggccgcatcg
     6421 agcgcacgct gttcatcctc gactggctgc aaagcgtcga gctacgccgc cgcgtgcatg
     6481 ccgggctgaa caagggcgag gcgcgcaatg cgctggcccg tgccgtgttc ttcaaccgcc
     6541 ttggtgaaat ccgtgaccgc agtttcgagc agcagcgcta ccgggccagc ggcctcaacc
     6601 tggtgacggc ggccatcgtg ctgtggaaca cggtctacct ggagcgtgcg gcgcatgcgt
     6661 tgcgcggcaa tggtcatgcc gtcgatgact cgctattgca gtacctgtcg ccactcggct
     6721 gggagcacat caacctgacc ggtgattacc tatggcgcag cagcgccaag atcggcgcgg
     6781 ggaagttcag gccgctacgg cctctgcaac cggcttagcg tgctttattt tccgttttct
     6841 gagacgaccc ctattatggc gaaggtatca ttcaggaagc gttgatgaag gattatctct
     6901 gtaaaaagtt attcaaccgg ctttccggta ccctggtgat cagagcgcgc tgcggcaata
     6961 acattactgg cctggcatgt tgcaatattc tttatccctc gccccgatac agcggtcagc
     7021 tgcatattaa agagctgtat gtttctcagt gcgatcgaaa taagggcacg ggtaaagcga
     7081 taatgcgctt tatagcccgg cttgcgcttg aacaggaatg ccttagcctt agctggaacg
     7141 ctgaaaaatc caaccccggc gctaaccgtt tttatcaggc tctgggaggc agaataaatg
     7201 atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt
     7261 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     7321 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     7381 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc
     7441 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta
     7501 attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg
     7561 ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg
     7621 cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat
     7681 tggctgagac gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac
     7741 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
     7801 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
     7861 cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat
     7921 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
     7981 cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa
     8041 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
     8101 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
     8161 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
     8221 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
     8281 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg
     8341 cgtc
//
LOCUS       DADLTF010000157         8318 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.159, whole genome shotgun sequence.
ACCESSION   DADLTF010000157 DADLTF010000000
VERSION     DADLTF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.159"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            332..3181
                     /locus_tag="JN749_003496"
     CDS             332..3181
                     /locus_tag="JN749_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7369775.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3281..5209)
                     /gene="dhaR"
                     /locus_tag="JN749_003497"
     CDS             complement(3281..5209)
                     /gene="dhaR"
                     /locus_tag="JN749_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HAY7369776.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSA
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDAGQFKRRV"
     gene            5428..6498
                     /gene="dhaK"
                     /locus_tag="JN749_003498"
     CDS             5428..6498
                     /gene="dhaK"
                     /locus_tag="JN749_003498"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAY7369777.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDVPVHTPALNWGK"
     gene            6509..7141
                     /gene="dhaL"
                     /locus_tag="JN749_003499"
     CDS             6509..7141
                     /gene="dhaL"
                     /locus_tag="JN749_003499"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HAY7369778.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7152..>8318
                     /gene="dhaM"
                     /locus_tag="JN749_003500"
     CDS             7152..>8318
                     /gene="dhaM"
                     /locus_tag="JN749_003500"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7369779.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2173 a         2066 c         2076 g         2003 t
ORIGIN      
        1 cataactaat taggattatt acaaaaaatc ttcaccaaat tttttaaccg ctgtttgttg
       61 taatattttt gccatattca tagtaacgaa ttggaataag taacgacaac ttagggaata
      121 actcgatata aactcacctt tgtaaggggg gtgttaaata tatattaatt cctgtgttcc
      181 tgattttgcc cggtaataga gaattattct cagagaagct catcatgatt gcaccataca
      241 gatatcatga tgataagagg atattattca ttgacttaaa caaaatgaca aaatccatag
      301 acccatagcc tgggtactat ataggagtgg tatgggcatc aaacaacaca atgggaatac
      361 caaagccgat cgtctcgctg aattaaaaat ccgttcgccc tcaattcaac tgataaaatt
      421 tggcgctatt ggtttgaatg caattatctt ttcccccctg ctgatagctg ctgatacagg
      481 aagtcaatat ggcaccaata ttactattaa tgatggtgac agaattacag gagataccgc
      541 cgatccatca ggaaacctct atggtgtaat gaccccagca ggaaacacgc ctggcaatat
      601 caacctgggt aatgatgtca ccgtcaatgt caacgacgcc tctggatatg caaaaggaat
      661 cattattcag ggcaaaaaca gctccctgac agctaaccga ctcacagtag atgttgttgg
      721 tcaaacctct gccatcggca ttaacttaat tggtgactat acccatgctg acttaggcac
      781 aggcagcacc attaagagta acgatgacgg catcattatt gggcatagct caacactaac
      841 agccactcaa ttcaccattg aaaactcgaa cggtataggc ctaaccatca atgactatgg
      901 caccagtgtc gatcttggaa gcggaagtaa aatcaagacc gatggaagta caggtgttta
      961 tatcggtggt ctcaacggca ataacgccaa tggtgctgcg cgttttacgg cgacagacct
     1021 gacaatcgat gttcagggct acagcgccat ggggataaac gtacagaaaa actctgttgt
     1081 cgatctcgga acaaacagta ccattaaaac caatggcgat aatgctcacg gcctctggag
     1141 ctttggccag gttagcgcga atgcactcac tgttgatgta actggagccg cggccaatgg
     1201 cgtcgaagtt cgtggtggta caaccactat cggtgcagat agccatattt cttccgcgca
     1261 gggcggtggc ctcgtcacca gtggttcaga cgcgacaatc aatttttctg gcacggcagc
     1321 gcaacgaaac agcatctttt ccggcggttc ttatggtgcc tcggcccaga cggcaacggc
     1381 tgttatcaac atgcaaaata ccgatattac ggttgatcgt aatggcagtc tggcgctggg
     1441 tttgtgggcg ctcagcggcg gtagaataac cggagacagt ttggctatca ccggcgcggc
     1501 aggagccaga gggatttatg ccatgaccaa cagccagatc gacctcacga gcgatctggt
     1561 cattgatatg agtacacccg accagatggc catcgcaacg caacatgacg atggttatgc
     1621 caccagccgc atcaacgcct cgggtcgtat gcttatcaac ggtagcgttc tttccaaagg
     1681 tgggctaatc aatctggata tgcaccctgg gtcggtttgg acaggttcct ccctcagcga
     1741 taatgtcaat ggcgggaaac tggacgttgc aatgaataac agcgtctgga acgtaacaag
     1801 taattctaat ctcgacacgc tggcgctgag ccattcaact gtcgattttg ccagccacgg
     1861 gtcaactgcc ggcacattta ccacattaaa cgtagagaac ctgagcggta acagtacctt
     1921 tattatgcgt gctgatgttg ttggcgaggg taatggcgtt aataatagag gggatttatt
     1981 gaatatcagc gggagtagtg ctggtaatca cgtattggct atccgcaacc agggcagcga
     2041 ggccacaacg ggaaatgaag ttctgacagt ggtaaaaacc actgacggcg cggcctcgtt
     2101 cagcgcgtct tctcaggttg agttgggggg atatctgtac gatgtgcgta aaaatggcac
     2161 taactgggag ctttacgctt ccgggacagt tccggaaccg actcctaatc ctgaacccac
     2221 accagctccc gctcagcctc ccatagtcaa ccccgatcct acgcctaaac ccaccacgac
     2281 cgcagatgct ggcggcaatt atctcaatgt cggttactta ttgaactatg ttgaaaaccg
     2341 tacgctgatg caacggatgg gtgacttgcg aaatcagagt aaagacggta atatctggtt
     2401 gcgcagttat gggggaagcc tggactcctt tgccagtggc aaactgagcg gctttgacat
     2461 gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa tgccgttgta
     2521 tgtcggtctg tatattgact caacacatgc atcgccggac tatagcggag gcgacggtac
     2581 cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacaaaacg gtttttacag
     2641 cgatctcgtt ataaaagcat cgcgccagaa aaatagtttc cacgtactgg acagtcagaa
     2701 caactgcgtt aacgccaacg gcactgcgaa tggaatgagc atctccctgg aagccgggca
     2761 gaggttcaac ctgtccccta ctggttatgg gttctatata gagccgcaaa cccagcttac
     2821 atacagccac cagaatgaga tggctatgaa ggcgagtaat ggcctcaata tacatctgaa
     2881 tcactacgaa tcgctgctgg ggcgtgccag catgatactg gggtatgaca tcaccgcagg
     2941 caacagccag ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt caggggatac
     3001 cgaatatctg ttgaacgact cccgggagaa gtacagtttc aaaggtaatg gctggaataa
     3061 cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctatctcg aagcggatta
     3121 cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcgct tcagctttta
     3181 agtgcgggat agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg ccggaatgaa
     3241 atacagatca ctctgatgta tcagaaatcg tttctgtctt tcatacccgg cgcttaaact
     3301 gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg ccgatcccaa
     3361 gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta ataattgcct
     3421 ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg acatcatctg
     3481 tcgcctgctc ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc ccgttatcac
     3541 tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcct ggccatgcac
     3601 aagaaactag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag aagcgttttt
     3661 caagactgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc atacgcagag
     3721 gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta aaacgatttt
     3781 gttccaccag cattgcgagg tccgcggtcg ttgttgcaat cactttgaca tcaattggta
     3841 ttaaacgccg cgcatccagt cgcgtgataa ccccctgctt gataacctgc agcaaagcag
     3901 actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg ccgtgtgcca
     3961 gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca ataaattctt
     4021 ccgccagcgc agcatcacca tataactcac aattgacggc gatataagga cctgcagcac
     4081 gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc tcttctccac
     4141 aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc aagcggcggg
     4201 tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct aattgactgg
     4261 tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg gttccctgcg
     4321 tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg ctttcaaagg
     4381 ttgcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat acggcgggta
     4441 acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg cgcaagaccc
     4501 gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta atcacgccat
     4561 catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt tcagcgagca
     4621 aactgtccgt cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc aacggcaaat
     4681 cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc aatcggccct
     4741 tgctgtcaaa caacggcgtt gcacaaaagg cccagttcca gagtgcctgt ttgaaatgtt
     4801 gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc gcacaagtac
     4861 caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt agctgctgca
     4921 aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg ataaacagcg
     4981 cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc ccgagcgtca
     5041 gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc tgatgtggta
     5101 cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt gcaattaagg
     5161 gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc ccttatgaca
     5221 aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca atatggaaca
     5281 tttattaaag catatgtttc actatgaaac ataatttggg ctaattttct tttcccttgc
     5341 ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac atcatccatg
     5401 ccctaccgta attgctggcg caaaataatg aaaaaattga tcaatgatgt gcaagacgta
     5461 ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact gcatcaggat
     5521 ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct gctgtcgggt
     5581 ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat gctttcgggg
     5641 gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga atgctccatg
     5701 caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg cgatattctt
     5761 aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac cactgtggtc
     5821 attgatgacg acgttgcggt aaaagacagt ctttataccg ccgggcgacg cggcgttgcc
     5881 aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga ctcactggac
     5941 gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg tatcgctctc
     6001 ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga taatgagatg
     6061 gagtttggcg tcggcattca tggtgagcca ggtattgacc gccgcccttt ctcttccctt
     6121 gatcaaaccg tcgatgaaat gttcgacacc ctgctggaac atggctcata ccatcgcact
     6181 ttgcgtttct gggattatca acaaggcagc tggcaggaag aaccacaaac caaacaaccg
     6241 ctccagtctg gcgatcgggt gattgcactg gttaacaatc ttggcgcaac tccgctttct
     6301 gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg attgactatc
     6361 gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt ctcaatcacc
     6421 ttactgaaag ttgatgacga aacgctggct ctctgggacg tcccggtcca caccccggcc
     6481 cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa ttgttaactg
     6541 gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg ggctggatcg
     6601 cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttagca aagtggtgga
     6661 aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaaaaata ccggtatgac
     6721 gctgctttcc agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct ttatccgcgc
     6781 cgcacaggcg acccaggcac ggcaaagcct gacactggaa gagctttatc agatgttccg
     6841 cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata aaaccatgtg
     6901 tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa atctctctgt
     6961 tccggtggcg ctcgaagctg ccagtagcat cgccgaatcc gcggcacaaa gtacgattac
     7021 gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg gtcaccagga
     7081 tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg caaaagagta
     7141 aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg ggagaaggtg
     7201 tcggtgaatt agcccgtcag atgttaatga gtgatagttg taaaatcgcc attgccgcgg
     7261 gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg gaggccatcg
     7321 aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc gcattattga
     7381 gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta cgtttgtgtg
     7441 ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg ggggcggata
     7501 tcgacaaagt tatctttgac gctatgcatg cgctggaagc caaacgtgaa caactgggtt
     7561 taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa gaagcccgtt
     7621 ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc tcccggctgg
     7681 tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc aaatgcgtca
     7741 caccagagag tattaaccag attgcgttac tacaagttcg ctataacgat acgctgcgcc
     7801 tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcag ctggctgaag
     7861 ataactttgg tgaaacggag gaagtcgctc cacctactct gcgtcccgtt ctgcctgttt
     7921 cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa tcaaccctga
     7981 ccgcggaaga agaacaagat cgattacgcc aggctattga cttcacgtta ttagatctga
     8041 tgacgttaac agcgaaagca gaagccagcg ggcttgacga tattgccgca atcttttctg
     8101 gtcaccatac actgttaggt gatccggaac tgctggcggc ggcaagcgaa ctccttcagc
     8161 atgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt agccagcaat
     8221 accagcaact ggatgatgaa tatctacaag ctcgctatat tgatgtggac gatcttctgc
     8281 atcgcaccct ggtccacctg acccaaacga aagaagaa
//
LOCUS       DADLTF010000158         8240 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.160, whole genome shotgun sequence.
ACCESSION   DADLTF010000158 DADLTF010000000
VERSION     DADLTF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.160"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..839)
                     /locus_tag="JN749_003501"
     CDS             complement(<1..839)
                     /locus_tag="JN749_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HAY7369780.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STP"
     gene            1118..2071
                     /gene="tal"
                     /locus_tag="JN749_003502"
     CDS             1118..2071
                     /gene="tal"
                     /locus_tag="JN749_003502"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HAY7369781.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2186..2773
                     /gene="mog"
                     /locus_tag="JN749_003503"
     CDS             2186..2773
                     /gene="mog"
                     /locus_tag="JN749_003503"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HAY7369782.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2808..3374)
                     /gene="satP"
                     /locus_tag="JN749_003504"
     CDS             complement(2808..3374)
                     /gene="satP"
                     /locus_tag="JN749_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HAY7369783.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3523..4236)
                     /gene="msyB"
                     /locus_tag="JN749_003505"
     CDS             complement(3523..4236)
                     /gene="msyB"
                     /locus_tag="JN749_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HAY7369784.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4262..4666)
                     /locus_tag="JN749_003506"
     CDS             complement(4262..4666)
                     /locus_tag="JN749_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HAY7369785.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            5043..6959
                     /gene="dnaK"
                     /locus_tag="JN749_003507"
     CDS             5043..6959
                     /gene="dnaK"
                     /locus_tag="JN749_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAY7369786.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            7048..8178
                     /gene="dnaJ"
                     /locus_tag="JN749_003508"
     CDS             7048..8178
                     /gene="dnaJ"
                     /locus_tag="JN749_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAY7369787.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
BASE COUNT         2185 a         2059 c         2162 g         1834 t
ORIGIN      
        1 tttggcgtcg atcccattcc cgcctcattg gaaaacatac tgcgctgaaa accgttagta
       61 atcgcctggc ttaaggtata tcccgccgcg ccgcctgccg cttcctgcca gccaaaagca
      121 ctctcaaaaa tagaccaaat gacgtgggga agttgcccaa tatttattac gcaaatcacc
      181 aggctggtca gtacccagat tatcgccatc aacgggacaa acccctgcat gagccgggcg
      241 acgccatgaa gaccgcgagt gattgccagc agagcaaaga cagcgagaat aatgcccgtc
      301 accagcggag gaaaatcaaa agaaaaactc agggctcggg caacggcgtt cgcctgaact
      361 ccgctgaaaa ttatgccgta ggcgatgagc aaaaagacgg cgaacagaac gcccatccag
      421 cgcatcccca gcccgcgcgc catataccat gccggtccgc cacgaaactg cccattgacg
      481 tcacgttctt tataaagttg tgcaagggaa cattcggcaa acgaggtcgc catgccgata
      541 aacgcagcaa cccacatcca aaagacggca cctggtccac cggcggtaat tgccagcgca
      601 acgccggcca ggttgccgct acccacgcgc gccgcaagac tggtacacaa tgactgaaat
      661 gaggttaaac cgcctggctg tggatgaatg ctatttttaa gacttttgcc aaactggcgg
      721 atgtagcgaa actgcacaaa tccggtgcga aaagtgaacc aacaacctgc gccgaagagc
      781 aggtaaatca ttaccgatcc ccaaaggacg ctgttaataa aggagaaaaa atctggcatg
      841 catatccctc ttattgccgg tcgcgatgac tttcctgtgt aaacgttacc aattgtttaa
      901 gaagtatata cgctacgagg tacttgataa cttctgcgta gcatacatga ggttttgtat
      961 aaaaatggcg ggcgatatca acgcagtgtc agaaatccga aacagtctcg cctggcgata
     1021 accgtcttgt cggcggttgc gctgacgttg cgtcgtgata tcatcagggc agaccggtta
     1081 catcccccta acaagctgtt taaagagaaa tactatcatg acggacaaat tgacctccct
     1141 tcgtcagtac accaccgtag tggccgacac tggggacatc gcggcaatga agctgtatca
     1201 accgcaggat gccacaacca acccttctct cattcttaac gcagcgcaga ttccggaata
     1261 ccgtaagttg attgatgatg ctgtcgcctg ggcgaaacag cagagcaacg atcgcgcgca
     1321 gcagatcgtg gacgcgaccg acaaactggc agtaaatatt ggtctggaaa tcctgaaact
     1381 ggttccgggc cgtatctcaa ctgaagttga tgcgcgtctt tcctatgaca ccgaagcgtc
     1441 aattgcgaaa gcaaaacgcc tgatcaaact ctacaacgat gcaggtatta gcaacgatcg
     1501 tattctgatc aaactggctt ctacctggca gggtatccgt gctgcagaac agctggaaaa
     1561 agaaggtatc aactgtaacc tgaccctgct gttctccttc gctcaggctc gtgcttgtgc
     1621 ggaggcgggc gtgttcctga tctcgccgtt tgttggccgt attcttgact ggtacaaagc
     1681 gaataccgat aagaaagagt acgctccggc agaagatccg ggcgtggttt ctgtatctga
     1741 aatctaccag tactacaaag agcacggtta tgaaaccgtg gttatgggcg caagcttccg
     1801 taacatcggc gaaattctgg aactggcagg ctgtgaccgt ctgaccatcg caccggcact
     1861 gctgaaagag ctggcggaga gcgaaggggc tatcgaacgt aaactgtctt acaccggcga
     1921 agtgaaagcg cgtccggcgc gtatcactga gtccgagttc ctgtggcagc acaaccagga
     1981 tccaatggca gtagataaac tggcggaagg tatccgtaag tttgctattg accaggaaaa
     2041 actggaaaaa atgatcggcg atctgctgta atcattctta gcgtgaccgg gaagtcggtc
     2101 acgctacctc ttctgaagcc tgtctgtcac tcccttcgca gtgtatcatt ctgtttaacg
     2161 agactgttta aacggaaaaa tcttgatgaa tactttacgt attggcttag tttccatctc
     2221 tgatcgcgca tccagcggcg tttatcagga taaaggcatt ccagcgctgg aagaatggct
     2281 gacatcggcg ctaaccacgc cgtttgaact ggaaacccgc ttaatccccg atgagcaggc
     2341 gatcatcgag caaacgttgt gtgagctggt ggatgaaatg agttgccatc tggtgctcac
     2401 cacgggcgga actggcccgg cgcgtcgtga cgtaacgccc gatgcgacgc tggcagtagc
     2461 ggaccgcgag atgcctggct ttggtgaaca gatgcgccag atcagcctgc attttgtacc
     2521 aactgcgatc ctttcgcgtc aggtgggcgt gattcgcaaa caggcgctga tccttaactt
     2581 acccggtcag ccgaagtcta ttaaagagac gctggaaggt gtgaaggacg ctgagggtaa
     2641 cgttgtggtg catggtattt ttgccagcgt accgtactgc attcagttgc tggaagggcc
     2701 atacgttgaa acggcaccag aagtggttgc tgcattcaga ccgaagagtg caagacgcga
     2761 cgttagcgaa taaaaaaatc cccccgagcg gggggatctc aagacaatta gtgggattca
     2821 ccaatcggca gaacggtgcg accaaactgc tcgttcagca cttcacccat cgccagatag
     2881 attgcgctgg caccgcagat cagaccaatc cagccggcaa agtggatgat cgcggcatta
     2941 ccggcaatgt taccgatcgc cagcagggca aacagcacgg tcaggctaaa gaaaacgaat
     3001 tgcaggacgc gtgcgccttt cagagtgccg aagaacataa acagcgtaaa tacgccccac
     3061 agacccaggt agacaccaag gaactgtgca tttggcgcat cggtcagacc cagtttcggc
     3121 atcagcagaa tcgcaaccag cgtcagccag aaagaaccgt aagaggtgaa tgcggttaaa
     3181 ccgaaagtgt tgcctttttt gtactccagc agaccagcaa aaatttgcgc gatgccgccg
     3241 tagaaaatgc ccatggcaag aataataccg tccagagcga aataacccac gttgtgcagg
     3301 ttaagcagaa tggtggtcat gccgaagccc atcaggccca gcggtgccgg attagccaac
     3361 ttagtgttgc ccataattcc tcaaaaatca tcatcgaatg aatggtgaaa taatttccct
     3421 gaataactgt agtgttttca gggcgcggca taataatcag ccagtggggc agtgtctacg
     3481 atcttttgag gggaaaatga aaattttccc cggtttccgg tatcagacct gagtggcgct
     3541 aaccatccgg cgcaggcagg cgatttgcag tacggctgga atcgtcacgc gataggcgct
     3601 gccgctgacc gctttaaccc catttaatgc tgcgcccaca gggcctccca gccccgctcc
     3661 gcgcagcaaa ccatgcccaa gtacgctcat tgctgcgtgg gtgcgtaaaa tgcgggtcag
     3721 ttggctggaa agcaaatgcg acacaccttt tgccaataat ttgtctttca tcagcagcgg
     3781 cagcagctct tccagctcat tcaccctggc atcgaccgcg tgcagaaact cctgcttatg
     3841 ttcctcgtcc attttcttcc aggtattacg cagaaattgt tccagtaact gttgctcaat
     3901 ttcaaacgta gacatctctt tgtcggcttt cagcttcaat cgctttgaaa catcgagcaa
     3961 aatggcccga tacaatttac cgtgtccgcg cagtttgttg gcgatactat cgccaccaaa
     4021 atgctgtaat tctccggcaa tcagctgcca gttgcggcga tgttgctcgg gatgcccttc
     4081 catcgattta aacagttcgt tgcgcatcag tacgctggag aggcgagttt tgcctttttc
     4141 attatgggtg agcaatcggg cgaaatttgc caactgttcc tcactacaat gctgaagaaa
     4201 atccagatct gaatcattca ggtaattaac attcattttt tgtggcttct atattctggc
     4261 gttagtcgtc gccgataatt ttcagcgtgg ccatatccga tgagttcacc gtatgaccgg
     4321 aaaaggtgat ttttgagacg cagcgtttat tgtcgttatc gctgttaatg ttgatccagt
     4381 cagtggtttg cccttctttt atttctgaag gaatattcag gctctgactg gcgctacggg
     4441 cggctttgaa ataaaccgat gcaccgctta actgtaaatc gccatggtcg gcagagagtt
     4501 gtatgcgttt cacaatgcga caaacaggaa gtttcagcgc cagatcgttg gtttcgttac
     4561 gcggcattgc aatgacgccg aggagtttat ggtcgtttgc ctgcgccgtg cagcacagca
     4621 tcaggctaat cgccaggctg gcggaaatcg taaaaacgga tttcataagg attctcttag
     4681 tgggaagagg tagggggatg aatacccact agtttactgc tgataaagag aagattcagg
     4741 cacgtaatct tttcttttta ttacaatttt ttgatgaatg ccttggctgc gattcattct
     4801 ttatatgaat aaaattgctg tcaattttac gtcttgtcct gccatatcgc gaaatttctg
     4861 cgcaaaagca caaaaaattt ttgcatctcc cccttgatga cgtgggttac gaccccattt
     4921 agtagtcaac cgcagtgagt gagtctgcaa aaaaatgaaa ttgggcagtt gaaaccagac
     4981 gtttcgcccc tattacagac tcacaaccac atgatgaccg aatatatagt ggagacgttt
     5041 agatgggtaa aataattggt atcgacctgg gtactaccaa ctcttgtgta gcgattatgg
     5101 atggcaccat tcctcgcgtg ctggagaacg ccgaaggcga tcgcaccacg ccttctatca
     5161 ttgcctatac ccaggatggt gaaactctgg ttggtcagcc ggctaaacgt caggcagtga
     5221 cgaacccgca aaacaccctg tttgcgatta aacgcctgat tggccgccgc ttccaggacg
     5281 aagaagtaca gcgtgatgtt tccatcatgc cgttcaaaat tattgctgct gataacggcg
     5341 acgcatgggt cgaagttaaa ggccagaaaa tggcaccgcc gcagatttct gctgaagtgc
     5401 tgaaaaaaat gaagaaaacc gctgaagatt acctgggtga accggtaact gaagctgtta
     5461 tcaccgtacc ggcatacttt aacgatgctc agcgtcaggc aaccaaagac gcaggccgta
     5521 tcgctggtct ggaagtaaaa cgtatcatca acgaaccgac cgcagctgcg ctggcttacg
     5581 gtctggacaa aggcactggc aaccgtacta tcgcggttta tgacctgggg ggtggtactt
     5641 tcgatatttc tattatcgaa atcgacgaag ttgacggcga aaaaaccttc gaagttctgg
     5701 caaccaacgg tgatacccac ctgggtggtg aagacttcga cagccgtctg atcaactatc
     5761 tggttgaaga attcaagaaa gatcagggca ttgacctgcg caacgatccg ctggcaatgc
     5821 agcgcctgaa agaagcggca gaaaaagcga aaatcgaact gtcttccgct cagcagaccg
     5881 acgttaacct gccatacatc actgcagacg cgaccggtcc gaaacacatg aacatcaaag
     5941 tgactcgtgc gaaactggaa agcctggttg aagatctggt aaaccgttcc attgagccgc
     6001 tgaaagttgc actgcaggat gctggcctgt ccgtatctga tatcgacgac gttatcctcg
     6061 ttggtggtca gactcgtatg ccaatggttc agaagaaagt tgctgagttc tttggtaaag
     6121 agccgcgtaa agacgttaac ccggacgaag ctgtagcaat cggtgctgct gttcagggtg
     6181 gtgttctgac tggtgacgta aaagacgtac tgctgctgga cgttaccccg ctgtctctgg
     6241 gtatcgaaac catgggcggt gtgatgacga cgctgatcgc gaaaaacacc actatcccga
     6301 ccaagcacag ccaggtgttc tctaccgctg aagacaacca gtctgcggta accatccatg
     6361 tgctgcaggg tgaacgtaaa cgtgcggctg ataacaaatc tctgggtcag ttcaacctag
     6421 atggtatcaa cccggcaccg cgcggcatgc cgcagatcga agttaccttc gatatcgatg
     6481 ctgacggtat cctgcacgtt tccgcgaaag ataaaaacag cggtaaagag cagaagatca
     6541 ccatcaaggc ttcttctggt ctgaacgaag atgaaatcca gaaaatggta cgcgacgcag
     6601 aagctaacgc cgaagctgac cgtaagtttg aagagctggt acagactcgc aaccagggcg
     6661 accatctgct gcacagctcc cgtaagcagg ttgaagaagc aggcgacaaa ctgccggctg
     6721 acgacaaaac tgctatcgag tctgcgctga ctgcactgga aactgctctg aaaggtgaag
     6781 acaaagccgc tatcgaagcg aaaatgcagg aactggcaca ggtttcccag aaactgatgg
     6841 aaatcgccca gcagcaacat gcccagcagc agactgccgg tgctgatgct tctgcaaaca
     6901 acgcgaaaga tgacgatgtt gtcgacgctg aatttgaaga agtcaaagac aaaaaataat
     6961 cgccttataa acgggtaatt atactgacac gggcgaaggg gaatttcctc tccgcccgtg
     7021 cattcatcta ggggcaattt aaaaaagatg gctaagcaag attattacga gattttaggc
     7081 gtttccaaaa cagcggaaga gcgtgaaatc agaaaggcct acaaacgcct ggccatgaaa
     7141 taccacccgg accgtaacca gggtgacaaa gaggccgagg cgaaatttaa agagatcaag
     7201 gaagcttatg aagttctgac cgactcgcaa aaacgtgcgg catacgatca gtatggtcat
     7261 gctgcgtttg agcaaggagg catgggcggc ggtggttttg gcggcggcgc agacttcagc
     7321 gatatttttg gtgacgtttt cggcgatatt tttggcggcg gacgtggtcg tcaacgtgcg
     7381 gcgcgcggtg ctgatttacg ctataacatg gagctcaccc tcgaagaagc tgtacgtggc
     7441 gtgaccaaag aaatccgcat tccgactctg gaagagtgtg acgtttgcca cggtagcggt
     7501 gcaaaaccag gtacacagcc gcagacctgt ccgacctgtc atggttctgg tcaggtgcag
     7561 atgcgccagg gattcttcgc tgtacagcag acctgtccac actgtcaggg ccgcggtacg
     7621 ctgatcaaag atccgtgcaa caaatgtcat ggtcatggtc gtgttgagcg cagcaaaacg
     7681 ctgtccgtta aaatcccggc aggggtggac actggagacc gcatccgtct tgcgggcgaa
     7741 ggtgaagcgg gcgagcatgg cgcaccggca ggcgatctgt acgttcaggt tcaggttaaa
     7801 cagcacccga ttttcgagcg tgaaggcaac aacctgtatt gcgaagtccc gatcaacttc
     7861 gctatggcgg cgctgggtgg cgaaatcgaa gtaccgaccc ttgatggtcg cgtcaaactg
     7921 aaagtgcctg gcgaaaccca gaccggtaag ctattccgta tgcgcggtaa aggcgtcaag
     7981 tctgtccgcg gtggtgcaca gggtgatttg ctgtgccgcg ttgtcgtcga aacaccggta
     8041 ggcctgaacg aaaagcagaa acagctgctg caagagctgc aagaaagctt cggtggccca
     8101 accggcgagc acaacagccc gcgctcaaag agcttctttg atggtgtgaa gaagtttttt
     8161 gacgacctga cccgctaacc tccccaaaag cctgcccgtg ggcaggcctg ggtaaaaata
     8221 gggtgcgttg aagatatgcg
//
LOCUS       DADLTF010000159         8216 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.161, whole genome shotgun sequence.
ACCESSION   DADLTF010000159 DADLTF010000000
VERSION     DADLTF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.161"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN749_003509"
     CDS             complement(18..1766)
                     /gene="msbA"
                     /locus_tag="JN749_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HAY7369788.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(1803..4022)
                     /locus_tag="JN749_003510"
                     /pseudo
     CDS             complement(1803..4022)
                     /locus_tag="JN749_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(4229..4513)
                     /gene="ihfB"
                     /locus_tag="JN749_003511"
     CDS             complement(4229..4513)
                     /gene="ihfB"
                     /locus_tag="JN749_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HAY7369789.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(4673..6346)
                     /gene="rpsA"
                     /locus_tag="JN749_003512"
     CDS             complement(4673..6346)
                     /gene="rpsA"
                     /locus_tag="JN749_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAY7369790.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(6457..7140)
                     /gene="cmk"
                     /locus_tag="JN749_003513"
     CDS             complement(6457..7140)
                     /gene="cmk"
                     /locus_tag="JN749_003513"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAY7369791.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(7313..8077)
                     /locus_tag="JN749_003514"
     CDS             complement(7313..8077)
                     /locus_tag="JN749_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7369792.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         2086 a         2168 c         1929 g         2033 t
ORIGIN      
        1 agaccagatt ttttcgatca ttggccaaac tgcattttgt gaagttgcgc gtaaacgccg
       61 cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa tgacaccatc ctcgacgacc
      121 acgatttcgt ctgccttttc aatggtagac aagcggtggg caatcaccag agaggtacgg
      181 tttttctgca actcatccag tgccgcctga atcgcacgtt cggattcggt atccagagcc
      241 gaggtagctt catccagaat cagaatcggg ctatcacgca gcaaggctcg agcgatagca
      301 atacgctgac gctgaccgcc agagagcagc acgccgtttt caccaatcac tgtatcgaga
      361 ccgttatcca tcttattgat gaagtccatg gcgtaggcca tacgcgccgc ttcttcaatt
      421 tgctcacggc tgtactgttc agtccgtgcg taagcaatgt tgttagcaac cgtatcgtta
      481 aacagatgga cattctgcga caccagagca acctggttac gtaacgacgc cagggtatac
      541 tcgcgcagat cgtgaccatc catcaggatt tcaccttcat caatatcgta aaaacgcgtg
      601 atcaggctgg caatggttga tttacctgaa ccagagcgtc caaccagagc aaccgtcttc
      661 cctgccggaa ttttcaggtt gatgttacgc aatgcgggta cgtcacgtcc cggataagta
      721 aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct cgatcacgcg cttaccttcg
      781 tctttctcct gctcactgtc cagaatggta aacagcgtct gacaagccgc cataccgcgc
      841 tggaactggg cgttaacgtt ggtcagcgat ttcagcggac gcatcagtgc aatcattgaa
      901 gagaaaacaa cggtaatcgt accggcagtc aggttatcca taacacttgg gaagctcgcc
      961 gcatacagaa caaacgccag cgccaaagag gcgatcagct gaatgatcgg atcagagatg
     1021 gaagaggctg aaaccatttt catcccctga agacgcattc ggttactgac tttatcgaag
     1081 cgtttcgttt ccacttcctg accaccgaaa atcaatactt ctttgtggcc cttcagcatt
     1141 tgttctgcac tggtggtcac ttgccccatg gtgttctgca tgtttttact gatgttgcga
     1201 aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg gtgccagcac aatcaaaatg
     1261 atcgacagtt gccaactgta atagaacatc atgatgaaca ggccgatgat cgacgcacct
     1321 tcacgcacaa cagtaatcag tgcgccggaa gaagaagaag caacctgttc ggaatcgtag
     1381 gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa agaatgaaac tggcattccc
     1441 atcatgtgac caaacaggcg gcgacgcatg gtcattacca cctttcctga tacccaggag
     1501 atacagtagc tggagacata gctggtgata ccacgtaaaa tcatcagtcc gatcaccacc
     1561 agcggcatcc acaccagcac ggagcgatct gttttaccaa agccatcatc aagaagtggc
     1621 ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga ggattaacgc tacgcccgcc
     1681 acgatcagac ccgctttgaa aggcgcaatg gttggccaca gtcggcggaa tgtctgccac
     1741 gtagagagat ctttgtcgtt atgcattcaa aaaaccagca tttgttgaaa tagccgcata
     1801 ttctacccgt tatccactgg cacgccaaac cactgataat accaacgagg taaaatttgc
     1861 tctctgaggc tgctaatccg ccagccttgt gctgaaaaat tgaccgtcac ttgaccctga
     1921 tgtggagtat caagccattg atatcctttc tgttgatagc gatgcttaac tttgttagag
     1981 ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
     2041 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
     2101 gcctgcactt gctgccagta acggcttagc atcttttgtt cagctggggc ttcaatatca
     2161 ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa ccacacagga atggttattt
     2221 cccttatcgt tgctaccttg taaaggccag tgcgcgctga aacgcaatcc ttgccactgc
     2281 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
     2341 aacatcggct atatatgcaa tattgaatcc agccctcccc ggtgatccag atgttcatgg
     2401 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
     2461 tgcccactat ccccttcagg ccaggccagt cctgtgtcat agagaatcgc tttgccgttt
     2521 ctggctatca ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca
     2581 ggtcgaggtt tttgccacag cggccagcac atcagcaagc ctccagccac acacattact
     2641 ggcaacgttc gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat
     2701 agccattgcc aacattcaac aatgttgatc cacccttccg gcaaactctt taacccctgt
     2761 tccaggatta acggcccgct aaaatgcaca accatcgcgg cgaggatcaa cggaaccgtg
     2821 ataaatgtca ccaagggaat tgcaaataga tttgcaataa acgaggtcag actaatgcca
     2881 tgaaatatga cgatttgcat gggcataagc aggagtgtga ttcccagttg cagatggatg
     2941 agggaaacaa ctgcacgcaa taccggcggc agttgccact caggacaggg aaaccactga
     3001 taccaaaaaa tcagtgccgc gaccgcagcg gcagagagcc ataaactttg cgagagaatg
     3061 gcaacaggat ccatcagcaa aattgccgcc agacaacata tccatacatc ccagccactc
     3121 cactgtcgcc cacttaactt aagcattccc cacgtagcaa gcgccaccac ggtacgcaat
     3181 gcaggaggtt gcattcccgt caaccaggca taaaaagcag cacagcagat tccgccaatt
     3241 aatggtattt gccagtggat ccagcgccca ggcagaaaaa tttgtccact gcgaatgagt
     3301 ccggcagcca gcaacgccgc aaaagcgatg tgcaatcccg atatcgccat taaatgcgcc
     3361 gttccagtat cgcgcatgat atttttgatt tctttgggga cggataaccg ttcccccata
     3421 cctaaaccaa gaataaccgc attccacgga tagggttgca gcgttgtttg tagtgacgcc
     3481 agatactgtg cacgcaggct acaattcggt tcaatgacac ttgcctgcag aaaacggccg
     3541 gtgagcggct gatgctgggc aatggcataa cgctggctat caaagccgcc atcattaagt
     3601 tggccatgaa ctgcacgaac tttgagtttc attgaccatt gttgtccagc acaaaccgct
     3661 tgcggaagtt attcgccata cagcacaagg cctggcgcag ggaatatacg tcgaccttgt
     3721 agataagtaa tttgaccgta atgagtggtc atgccgtcag ttgcagtgat ctcaacaatt
     3781 gcatcctgcg tcgcgccagt taaggtttct cctgcccaca aaatttgctt tgctgataat
     3841 atgccccaaa cgaaaaacag caacgtcagc gcgatataac ggacggtttt aacagggata
     3901 aatgccagta cgcaggcgaa gagagtcaga aacgcaaggg ttaatgtcct aggcaattgg
     3961 ggcaaaatca gcaacggaaa aattccgcaa attatgcata cgccgactgt cgttattttc
     4021 atgcccccct ccctgtttat ggaaagtgtg caactttgtt gagcggttgt ctggtgggaa
     4081 ttttgcgttc cccgaggcac attcagaaga atttaacgaa agggtgaaaa ctgctcaaaa
     4141 ctctgcgagg cagcttccag ttcctgatta gagaattgaa aaaagcaccc gacaggtgct
     4201 tttctcgcgt tcaagtttga gtaaaaactt aaccgtaaat attggcgcga tcgcgcagtt
     4261 ctttaccagg tttaaagtga ggaacgtatt ttccttccag ttctacttta tcgccagtct
     4321 tcggattacg tccggtacgt ggtgcgcggt agtgcaaaga gaaactgccg aaaccgcgga
     4381 tttcaatacg ctcgccctgc gcaagagtcg aggccatatg ctccagcatc tcttttactg
     4441 catcttcaac cgtcttggcg ggaatgtgcg attgctgggt ggcaagtctt tctatcaatt
     4501 ctgacttggt catgattcct ccggttcctt aaaggcaaat taagcggctg ctgttgctgc
     4561 attgattaag ggcggccgta gccgccctta gtgcttgatt acaggacgaa acctgcaatc
     4621 tgtcaagtaa actcaacaaa cttcggaata aaaatcccga agagtcagag aattactcgc
     4681 ctttagctgc tttgaaagct tcagccattg cgttgttgga gaagtttgca tcttcctgtt
     4741 tgttaacagt tgcgattgca tctttctcgt cagcttcgtc tttcgcacga acagacaggc
     4801 tgattgcgcg gtttttacga tcaacgccgg tgaatttagc ttcaacttcg tcgccaacgc
     4861 tcagaaccag ggtagcgtct tcaacgcggt cacgggatgc ttcagaagca cgcaggtaac
     4921 cttcaacgcc gtcagccagt tctacggttg cgcctttagc gtcaactgca gttactttac
     4981 cggttacgat agcgcctttc ttgttcagag caacccagtt gttgaacgga tcttctgcga
     5041 gctgtttaac gcccagggag atacgttcac gttctgcgtc aacctgcaga acaactgcag
     5101 cgatttcgtc gccttttttg tattcacgaa ctgcttcttc gcctgcaacg ttccaggaga
     5161 tgtcagacag gtgaaccagg ccgtcgatgc cgccgtccag gccgatgaag ataccgaagt
     5221 cagtgataga cttgatttta ccttcaacac ggtcgccctt gttgtgggtt tccgcgaact
     5281 gctgccacgg gttagctttg cactgtttca gccctaggga gatacgacga cgttcttcgt
     5341 cgatatccag aaccataact tccactacat cgccaacgtt aacaactttg gacgggtgga
     5401 tgtttttgtt ggtccaatcc atttcggaaa cgtgtaccag accttcaacg ccttcttcga
     5461 tttcaacgaa gcagccgtag tcagtcaggt tggtcacgcg accagtcagt ttggtacctt
     5521 ccggataacg tttagcgata gctacccacg gatcttcgcc cagctgtttc aagcccaggg
     5581 atacacgggt acgttcgcgg tcgaacttca gcactttaac agtgatttcg tcgcccacgt
     5641 tgacgatttc gctcggatgc ttaacgcgtt tccaggccat gtcagtgatg tgcagcaggc
     5701 cgtcaacgcc gcccagatca acgaatgcac cgtagtcagt gaggttctta acgatacctt
     5761 taacttccat gccttcctgc aggttttcca gcagctgatc gcgctctgcg ctgttttcgg
     5821 attcgataac ggcacgacga gaaacaacaa cgttgttgcg cttctgatcc agcttgatta
     5881 ctttaaattc aagctctttg ccttccaggt gcagagtgtc acgcaccgga cgaacgtcta
     5941 ccagagaacc aggcaggaac gcacgaatac cgttcagctc aacagtgaag ccgcccttaa
     6001 ctttgccgtt gataacaccg gtaacagttt cagcatcttc gtaagctttt tccagcgtga
     6061 tccaggcttc gtgacgttta gctttctcac gggacagcag agtttcaccg aagccgtctt
     6121 ctactgcgtc cagagcaacg tcaacttcgt cacctacctg gatttccagc tcgccctggg
     6181 cgtttttgaa ctgctcagcc gggatggcgg actcagattt cagaccagcg tcaaccagta
     6241 ctacgtcttt gtcgatagca acaacaacgc cacgaacgat agaacccggg cgggtttcga
     6301 tttcttttaa ggactcttca aagagttgag caaaagattc agtcatgttt aatcttcagg
     6361 tttatattta acgtccacct ggctccatgc cggatggggt tgtttaacat acccgctgtc
     6421 attccattgc aacgggggta ctgcaaattc ggtcgcttat gcgagagcca atttctggcg
     6481 cgcgtattgt agcgcttttt caatcacttg ctcaatgctt aaggtggtgg aatccaacac
     6541 taaagcatcg gctgccggaa ccagtggcgc taccgctcgg ttacgatcgc ggtcgtcgcg
     6601 ttctttgatc tcggccaaaa ggcgctcgaa gttaacacta aagcccttct cctgcaactg
     6661 tagcatgcgg cgatgcgcac gttcttccga ggaggcgtca aggaaaattt tcactggtgc
     6721 atcagggaat accaccgttc ccatgtcgcg gccatcggca atcagacctg gtaattcgcg
     6781 aaacgcgcgt tggcgacgca ataatgcttc acgaacgcgt gggaatgccg cgacttgtga
     6841 agccgcattc gccacttcct gagtacgaat ttcgccgctg acatcttccc cttcgaggat
     6901 cacttccaga ttgccattgg tcgacacaaa acgtacatcc agatgggatg ccagcggtac
     6961 cagcgcatct tccgacgcaa catcaacatg gtgatgtaat gccgccaatg ccagtacgcg
     7021 ataaattgca cccgagtcca gcagatgcca ttgcaacgct tccgccatag ccttacacaa
     7081 ggtgcctttc cctgcaccgc ttgggccatc aatggtaata accggggcaa ttgccgtcat
     7141 ctttatctcc ttaaaacagg cgtaccgtta acataaccgc gcgcattata cgcgccgacg
     7201 ccacaaatcg ttaacgttgt gtgcaaaaac cagttttgtc tgtattcaga tggggattta
     7261 ttgcgcaatt atagcgggct ggcagaagac cagcccgaaa gatgacaatg atttacttac
     7321 cagaggcgat acgatcacgg atgtgttgcg cacgctctgt tgatggtggg tgagagtcaa
     7381 acatggactg ggtgcgaccg ccatccaggc tagccagttt ttcaaagctg ccaaccagcc
     7441 cctgggtgct gatgccacgt ttcttcaaca gatcaaaaga gaaatcatct gcttcggact
     7501 ctttatcacg ggagtattta gcattaatag cgccttctgc gatatcaccc aattgtgagc
     7561 gggaaagctg ggaagccacg ccgctggtag ctgaaatggc atcgcgtgcg gcaacgatcg
     7621 cataagaagc tttcatttca gccagcgagt gacccaacgc gacgtggccc agttcatggc
     7681 ccagaacacc ttcaatttcg ttgtcattca tcatgtccat caggccactg tagacacgaa
     7741 cacagccgtt cgccatcgcc catgcgttga cgtcgctggt catataaacc ttatagttga
     7801 ccggcgtgcc attaatattg ttacccaatg ctttggcgat ttttgccaga cgtttaccgt
     7861 acttgctgga tttacttgcg acttgattgc cgctgtccat ttcggcacag ccctgattgg
     7921 caatcgcttt tgcatcggca tcgcttaatg ttgctgcttt ataggcattt aaaccggagc
     7981 tgatagccat attggtatca ataccgtggg tattttgaca ccctgtcagt aatgctgcag
     8041 aggtcgcaat cgccagcagt aatttagtat tcttcatgtg aatcatccgt tgattaatca
     8101 tatataaata ttatgtatca acaacttaca ttgacaagcg acgctaccat taatcaaaat
     8161 gactcacaag gtccgaaaaa aaacgccgac aatgtgccgt aatgccagtc agttaa
//
LOCUS       DADLTF010000160         8200 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.162, whole genome shotgun sequence.
ACCESSION   DADLTF010000160 DADLTF010000000
VERSION     DADLTF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.162"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            59..460
                     /locus_tag="JN749_003515"
     CDS             59..460
                     /locus_tag="JN749_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7369793.1"
                     /translation="MSSNFCERLEQWGNKSVNNGEERAIAVERIKEAYNSNMASLDLS
                     YLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNINLSVNKINTITFPKEPKLEN
                     IYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            570..1901
                     /locus_tag="JN749_003516"
     CDS             570..1901
                     /locus_tag="JN749_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HAY7369794.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYIPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2225..3613
                     /locus_tag="JN749_003517"
                     /pseudo
     CDS             2225..3613
                     /locus_tag="JN749_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
     gene            3695..7328
                     /locus_tag="JN749_003518"
                     /pseudo
     CDS             3695..7328
                     /locus_tag="JN749_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            7325..>8200
                     /gene="narH"
                     /locus_tag="JN749_003519"
     CDS             7325..>8200
                     /gene="narH"
                     /locus_tag="JN749_003519"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HAY7369795.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2191 a         2029 c         1911 g         2069 t
ORIGIN      
        1 ttgtgtaaaa aataatgcca gttatcaact taataacgca ttacctaaca aagaaaccat
       61 ctccagcaat ttttgtgaac gactagaaca atggggtaat aaatcggtta ataatggtga
      121 agaaagagca attgccgtgg agcgaattaa agaagcttac aattcgaaca tggcatctct
      181 tgatttgtca tatcttgatt tgagtgaatt accgcctatc ccctcgacag ttaatacgtt
      241 aaatctggaa aacaattgtc taacttgtct tgacttagct gacattgcca gtctcgttaa
      301 tatcaacctc agcgttaata aaattaacac gataaccttt ccaaaagaac caaaactgga
      361 aaacatttat attgatcata acaatctgga aagtttagat ttaaaaaatc agcattcatt
      421 ggttaatctg gaagcgcaaa ataacagcct aaaaaaatga tatttctgat agttataaac
      481 tgaaatttct taatcttgat tataacaagc tagcatcgct ggatgtctcc cggcaagaat
      541 ctctgattga gttaagtgcc catcacaata tgatcactga ccttatatta cacaatcatc
      601 ccaagatgaa aacaatcact ttaaacgaca accatattgc acatttaaac gccaaaaaca
      661 ctacaaaact ggaatattta aacttaagca ataacaattt actgccaacc aatgacattg
      721 atcaactaat atcatcaaaa catctttggc atgtattagt taacggcatc aacaatgatc
      781 cacttgcaca aatgcagtac tggactgcag taagaaatat aattgatgac actaatgaag
      841 tgaccattga tttatcagga cttaatttaa ccactcaacc accagggctg caaaacttca
      901 cctctatcaa tcttgataat aaccaactca cacattttga tgcaaccaac tacgatagac
      961 tcgtaaagct aagtctgaat agtaatgctc ttgagtcaat aaattttcct caaggcagaa
     1021 atgtaagtat tacacatata tctatgaata ataatgctct cagaaatatt gatatagata
     1081 ggctttcatc agttacttat tttagtgcgg cacataatca actagagttt gtgcaattag
     1141 aatcttgcga atggctgcaa tacctgaatc tcagccataa tcaattaact gatattgttg
     1201 caggtaataa aaatgaactc ttactgctgg atctatccca taataaacta acaagtttac
     1261 acaatgtctt atttcccaac ttgaatacgt tacttattaa caacaatttg ctttctgaaa
     1321 ttaaaatatt ctatagcaac ttccgcaatg ttcagacatt aaacgctgct aacaaccagt
     1381 tgaaatatat aaatcttgat ttcctgactt atcttccatc tatcaaaagt ttaagactgg
     1441 acaataataa aataacccac attgatacta ataatacatc cgatattgga actttattcc
     1501 ccataataaa acagagcaaa aacttaaatt ttttaaatgt ttctgggaag aacaattgcc
     1561 ctactatgca gctcatgtta tttaatttgt tttccccagc acttaagctt aatactggcc
     1621 tggcaattct ttcgcctggt gcatttgaag ttcactctga cggaatagat gtggataacg
     1681 aattgtttca ctatactatt aaaaaagcat atatcccata taatatacac acttacaaga
     1741 cagaggaagt tgtaaaccag aggaatataa aagttaaaaa catgacctta gatgaaataa
     1801 acaatactta ctataataac gattattaca atcaggcaat aagagaggaa ccgatagact
     1861 ttctggacag atcgttttcc tccaattcat ggccttttta gtgaataaat catcactaat
     1921 tctataagtg tctggtcttg ttgagaatat agttaatatg taacccttta gattcacgat
     1981 ctgaagggtt gaaacatgac atacgttcaa agattaaccc ccgtttgaag gttagcttgt
     2041 tagttacatt tagtaacaca tatctggcta cttaatcccc attctgcccc cgcggttacg
     2101 ctttcttatc tattctttca atcagcactt cacccaattc atccttaatt ctaataactg
     2161 gctattgcgc cttacctcct gttttgtctt ttttcattac caatgtgtgc atgtgaggaa
     2221 caatatggca ctgcaaaatg agaaaaatag tcgttatctt ttacgcgact ggaaaccaga
     2281 aaatccggcc ttctgggaaa ataaaggaaa acatattgct cgaagaaacc tctggatatc
     2341 agtcagttgt ctacttcttg ccttctgtgt ctagatgcta tttagcgcag ttaccgttaa
     2401 tctcaataaa atcggtttta attttactac cgatcaactc tttttattaa ccgcattacc
     2461 ctccgtttct ggcgcattat tgcgtgttcc ctactccttt atggtgccta tattcggtgg
     2521 acgccaatgg ccggttttta gtactgcaat cctgattatt ccttgcgtct ggctcggaat
     2581 tgccgtgcaa aatccgaata ctccttttgg gatatttatc gttatcgctt tgctatgcgg
     2641 ttttgcaggt gcaaactttg cttcgagcat gggcaatatc agtttcttct ttccaaaagc
     2701 caaacaaggg agcactcttg ggattaatgg cggattagga aacttaggtg taagtgtaat
     2761 gcagctggtt gcaccgctgg tcatttttgt acctgtattt gcctttctcg gcgtcaatgg
     2821 cgtaccgcag gccgacggtt cggtgatgtc gctggcgaat gccgcatgga tttgggtacc
     2881 gctactggcg attgccacga tcgccgcctg gtcagggatg aatgatatcg ccagttcacg
     2941 cgcctcaatt gccgaccagc tccctgtctt acaacgcctg catctctggc tgctgagcct
     3001 gctttacctt gccaccttcg gttcgtttat cggtttttct gcgggttttg ccatgctggc
     3061 aaaaacccag ttcccggatg tgaatattct gcgcctggcg ttctttggcc catttattgg
     3121 tgccatcgcg cggtcggttg gtggtgctat ttccgataaa ttcggcggcg tgcgggtgac
     3181 gttgatcaac tttattttta tggcgatttt cagtgccctg ctgttcctta ccttaccggg
     3241 cacaggctcc ggtaatttca tcgcctttta cgccgtattt atggggctgt ttctgaccgc
     3301 gggtctggga agtggttcta ctttccagat gatcgccgtc atctttcgcc agataaccat
     3361 ttatcgggtg aagatgaaag gcggtagtga tgagcaagct cagagagaag ccgtcaccga
     3421 aacggcggca gcgctgggct ttatctcagc cattggcgca gtgggcggct tttttattcc
     3481 gcaggcattt ggcatgtcgc tcaatatgac cggctctccg gtcggcgcga tgaaagtgtt
     3541 tttaatcttc tacatcgttt gtgtgctgct gacctggctg gtttatggtc ggcggaagtt
     3601 cagccaaaaa taagagatcg atgcctgatg tacaaccgtg tcgcatccga caaggcatca
     3661 gatcccaatt cattcctgga gcaggagtca tgtcatgagt aaacttttgg atcgctttcg
     3721 ctacttcaaa caaaagggcg aaacctttgc cgatggtcac ggacaggtga tgcatagcaa
     3781 ccgcgactgg gaggacagct atcgccagcg ttggcagttc gacaaaatcg tgcgttccac
     3841 ccacggcgtt aactgtacag gctcctgtag ctggaaaatc tacgttaaaa atggtctggt
     3901 gacctgggaa atccaacaga ccgactaccc gcgcactcgc cctgacctgc ccaatcatga
     3961 acctcgcggc tgcccgcgtg gcgcaagtta ctcctggtat ctttacagcg ctaaccgcct
     4021 gaaatacccg ctcattcgta aacgactgat tgaactgtgg cgcgaagccc tcaaacaaca
     4081 cagcgatccg gtactggcgt gggcatcgat tatgaacgat ccgcaaaagt gcctgagcta
     4141 caaacaagtg cgtgggcgcg gcgggtttat ccgctccaac tggcaggaac taaaccagct
     4201 gattgccgcc gctaacgtct ggaccatcaa aacctacggc ccggatcgcg ttgccggttt
     4261 ctcgccgatc ccggcgatgt cgatggtttc ttacgccgcc ggaacgcgtt atctgtcgct
     4321 gcttggcggc acctgtttaa gtttctacga ctggtattgc gacctgccgc ccgcctcgcc
     4381 gatgacctgg ggcgagcaaa ccgacgtacc ggaatctgcc gactggtata actccagcta
     4441 catcatcgct tgggggtcta acgtaccgca gacacgtacg ccggacgccc acttctttac
     4501 cgaagtacgc tacaaaggca ctaaaaccat cgccattacc cctgactact ctgaagtggc
     4561 caaattgtgc gaccagtggc tggcaccgaa acaaggcact gatagcgccc tggcgatggc
     4621 aatggggcat gtgattttaa aagagtttca tctcgataat cccagcgact actttatcaa
     4681 ctactgccgc cgctacagcg acatgccgat gctggtaatg ctggagcctc gcgacgatgg
     4741 tagctacgtt cccgggcgca tgatccgcgc atctgacctg gtggatggac tgggcgaaag
     4801 caacaatccg cagtggaaaa ccgtagcagt taataccgca ggtgaattgg tagtgccgaa
     4861 cggttcgatt ggtttccgct ggggagaaaa aggcaaatgg aatctggaat ccattgccgc
     4921 cggtacggaa accgaattgt cgttaaccct gctcggtcaa catgatgctg ttgcaggcgt
     4981 ggccttcccc tactttggcg gcattgaaaa tccgcatttt cgcagcgtaa aacacaatcc
     5041 ggtgctggtg cgccaattgc ccgttaaaaa cctgacgtta gtcgatggca acacctgtcc
     5101 agtggtcagc gtttatgatt tggtactggc gaattacggc ctcgatcgcg ggctggaaga
     5161 tgaaaacagc gcgaaagatt acgctgaaat caaaccgtac accccagcct ggggtgagca
     5221 aattaccggc gtaccgcgcc agtatattga aaccatcgcc cgcgaatttg ccgataccgc
     5281 ccataaaacg catgggcgct caatgattat cctcggcgca ggtgttaacc actggtatca
     5341 catggacatg aactaccgcg ggatgatcaa tatgctgatc ttctgcggtt gtgtcgggca
     5401 aagcggtggc ggctgggcac actatgtcgg ccaggaaaaa ctgcgcccac aaaccggctg
     5461 gttgccactg gcctttgcgc tcgactggaa ccgaccaccg cgccagatga acagcacctc
     5521 gtttttctac aatcattcca gccaatggcg ctatgaaaaa gtctctgctc aggagttact
     5581 ttcaccgctc gccgatgcca gtaagtacag cggtcatctg attgatttca acgttcgcgc
     5641 cgaacgtatg ggctggctac cttctgcgcc gcagctgggg cgtaacccgc tcgggattaa
     5701 agctgaagcc gacaaggccg gattatcccc cacagaattt accgcccagg cgctgaaatc
     5761 gggcgattta cgtatggcct gcgaacaacc agatagcggc agcaatcatc cgcgtaattt
     5821 gtttgtctgg cgctctaacc tgcttggctc ctccggcaaa ggccacgagt atatgcagaa
     5881 gtatctgctg gggaccgaaa gcgggattca gggcgaggaa ctcggtgcca gcgacgggat
     5941 caaaccggaa gaagtcgagt ggcaaactgc ggcgattgaa ggcaagctcg acctgctggt
     6001 gacgctcgac ttccgcatgt ccagtacctg cctgttctcc gatatcgtcc tgcccaccgc
     6061 cacctggtat gaaaaagacg atatgaacac ctcggatatg catccattta ttcatccgct
     6121 ttctgcggcg gtcgatccgg cctgggaatc acgcagcgac tgggaaatct acaaaggtat
     6181 tgccaaagca ttttcgcaag tgtgcgtggg ccatcttggc aaagaaaccg acgtggtatt
     6241 acaaccactg ctgcatgact ctccggcaga gctctcacag ccgtgtgaag tgctcgactg
     6301 gcgcaaaggc gaatgcgatc tgatcccggg taaaaccgcg ccgaatattg tggcggtgga
     6361 gcgcgactac cctgctacgt atgaacgctt tacctcgctc gggccattga tggacaaact
     6421 tggcaatggc ggtaaaggga tttcgtggaa tacccaggat gaaatcgatt tcctcggcaa
     6481 actcaattac accaagcgtg atggcccggc acaggggcgt ccgctgattg acaccgccat
     6541 tgacgcttca gaagtgattc tggcactggc accggaaacc aacggtcatg ttgcagtcaa
     6601 agcgtggcag gcgctgggcg agatcaccgg gcgcgaacat acccatctgg cgctgcacaa
     6661 agaggacgag aagattcgct ttcgcgatat tcaggcgcag ccgcgtaaaa ttatctccag
     6721 ccccacatgg tccggtctgg aaagcgatca cgtatcctat aacgcgggat acaccaacgt
     6781 tcatgagtta attccgtggc gcacgctgtc gggacgccag cagctctatc aggatcatcc
     6841 gtggatgcgt gcttttggtg aaagcctggt ggcttatcgc ccgcctatcg acacccgtag
     6901 cgtcagtgag atgcgccaga taccgccaaa cggcttccag gaaaaagcac ttaacttcct
     6961 gacgccgcac cagaaatggg gcattcactc aacctacagt gaaaacctgc taatgctgac
     7021 gctctctcgc ggtggtcagc caacgtgtac cgccgggcat gaccatgatg tatcacgccc
     7081 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     7141 actcggtcac ccgcgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     7201 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     7261 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     7321 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgataaat gtatcggctg
     7381 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     7441 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     7501 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatccgtc cgcgtctggg
     7561 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agcagattga
     7621 tgattactac gaacctttca ccttcgacta cgaacatttg catagcacac cggaaggcaa
     7681 acatatccct actgctcgcc cgcgttcact gattgacggc aaacggatgg acaaagtgat
     7741 ctggggccca aactgggaag aactgctggg cggcgagttt gaaaaacgtg cccgcgaccg
     7801 caacttcgag gccatgcaaa aggagatgta cgggcagttt gaaaacacct tcatgatgta
     7861 cctgccgcgc ctgtgcgaac actgcctcaa tcccagctgc gtggcgacct gcccaagcgg
     7921 cgctatctac aaacgcgaag aagacggcat tgtgctgatt gatcaggata aatgccgtgg
     7981 ctggcgtttg tgcattagcg gttgtccgta caaaaaaatc tacttcaact ggaaaagcgg
     8041 caagtcagaa aaatgtatct tctgttatcc gcgaattgag tcaggccaac ccaccgtgtg
     8101 ctcagaaacc tgcgtgggtc gcatccggta tctgggcgtg ctgctttacg acgccgaccg
     8161 catcgaggaa gcggcgagca ccgagcgcga agttgacctc
//
LOCUS       DADLTF010000161         8193 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.163, whole genome shotgun sequence.
ACCESSION   DADLTF010000161 DADLTF010000000
VERSION     DADLTF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.163"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JN749_003520"
     CDS             complement(<1..671)
                     /gene="chbR"
                     /locus_tag="JN749_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HAY7369796.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIIN"
     gene            complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JN749_003521"
                     /pseudo
     CDS             complement(679..1029)
                     /gene="chbA"
                     /locus_tag="JN749_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
     gene            complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JN749_003522"
                     /pseudo
     CDS             complement(1088..2436)
                     /gene="chbC"
                     /locus_tag="JN749_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JN749_003523"
     CDS             complement(2521..2841)
                     /gene="chbB"
                     /locus_tag="JN749_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HAY7369797.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JN749_003524"
     CDS             complement(3140..3478)
                     /gene="osmE"
                     /locus_tag="JN749_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HAY7369798.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3680..4507
                     /gene="nadE"
                     /locus_tag="JN749_003525"
     CDS             3680..4507
                     /gene="nadE"
                     /locus_tag="JN749_003525"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAY7369799.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINLLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4737..5624
                     /gene="cho"
                     /locus_tag="JN749_003526"
     CDS             4737..5624
                     /gene="cho"
                     /locus_tag="JN749_003526"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HAY7369800.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JN749_003527"
     CDS             complement(5584..6159)
                     /gene="ves"
                     /locus_tag="JN749_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HAY7369801.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREISTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JN749_003528"
     CDS             complement(6362..6847)
                     /gene="spy"
                     /locus_tag="JN749_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HAY7369802.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JN749_003529"
     CDS             complement(7177..8145)
                     /gene="astE"
                     /locus_tag="JN749_003529"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HAY7369803.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2152 a         2053 c         1893 g         2095 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcgcgtggc accaaactca
      421 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
      481 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
      541 tagtcgtgct gatgcagtcc gctgatacta tcagttttgt tatagataaa cacatggaag
      601 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
      661 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
      721 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
      781 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
      841 ttcattcaat gccatccgtg actgatccat catggctttc gctgcggcaa aatcgccctg
      901 cttcgcctgt ttcaatgcgg catacgccag gctacgcgct tgtccagagt tgatgataag
      961 ccccatcacc acttcttaca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     1021 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     1081 taaaatttca ggggttagcg atatcttctt cgctctcttc tttatcaatc gcattttgtg
     1141 ctttgttagc caccacaaca aagggcagat aaattaacgt tgcgatgcca aggttgaaga
     1201 gtgcgaccag caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg
     1261 gcatggtcca cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga
     1321 gggtgattgc cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca
     1381 tgataattgg cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg
     1441 ccagttttgc cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca
     1501 ggcctaaagt cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc
     1561 agatatggaa ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg
     1621 tcgcgatatt ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca
     1681 gcgcgccatg aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca
     1741 ccacgctacc caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt
     1801 tggtgcccca ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc
     1861 cgggaattaa tgccgagaaa gaacgcgata ctgaagcagg tacgctgtcg ggcagtttaa
     1921 tgacccaatt gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa
     1981 tcccggagat gatattcgcc ccacctaacc agtttgcgcc aaccgcatag gcctcaccga
     2041 cactatatgg ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat
     2101 cgactttacg ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta
     2161 ttcctaatgt tccgttatat acgttgccgc caataccttt cagaccatta agtgtttcaa
     2221 tggttgaggc gtcgaggcga atacctaagg aataaaaaac gacccctccc caaagcttag
     2281 aaaaacgttg ttaattaata caaacatggc ccccgcaagg gttaacggca ttaagcgaat
     2341 aaagccattt ttgattgcat taacgtgtgg ctgctttcct attttaactg caaaagggag
     2401 gagtaccttt tcaagcgatg caataacatt actcatagaa aaataccctt aaaaaccgca
     2461 atttaaatat tgcggtattg atttatgaaa taactctttg acgggaaaat ttaaaataaa
     2521 ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc ctaaaccatc
     2581 gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta acaaacgctg
     2641 gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg cattctgacc
     2701 tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt catatttttc
     2761 tgcctgtgcg cgcatttttg ataccagtaa agagatagac atgcccgcag aacaaaacag
     2821 ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc agccagacac
     2881 tccgcaagcc ttaacctgct tccatgctct gggtaacttg cgaaaccaac atgacttttt
     2941 ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt gaccttataa
     3001 attgtttctc cttgacctgt ggttatgacc ctcttcacat ttcgggcaaa taattcgcga
     3061 agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc gttgttactc
     3121 aagctttcag acgaattgat tacttcgcag cctgtggatc agtgtcgtat tcagcacagg
     3181 tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag taggtttctg
     3241 ctttaccatc acgttgaccc aggatgtagg tctggcaagt accgcgagca tggatcatgc
     3301 tcacttcaga cgaaggttta cccgcaattt gtgcaacctg cgcccggctc atgccttttt
     3361 tgacgtcttt caccacaggc tgtacaaact ggtctttggt acgatcataa gctgtacaac
     3421 ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc ttgttcatat
     3481 tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc gctttttcaa
     3541 gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgacgtttt aaccaccatt
     3601 cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa gttttgtctt
     3661 ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc tgggcgcaaa
     3721 accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga aaagctacct
     3781 gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc aggactccac
     3841 gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa ctggcaacga
     3901 atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggctgacg aacaagattg
     3961 ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata tcaagggcgc
     4021 ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg attttgtccg
     4081 tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg gtatgaccag
     4141 cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct tcactaaata
     4201 tggtgacggc ggtacggata ttaatctgct gtatcgtctc aacaaacgcc agggtaaaca
     4261 gttactgacg gcattaggtt gcccggaaca cctttataag aaagccccaa ctgccgatct
     4321 tgaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct atgacaatat
     4381 cgacgactat ctggaaggga aaaacctacc tgaacaggtc gccagaacaa tagagaactg
     4441 gtatctgaaa accgaacata aacgccgtcc gccaattaac gttttcgatg atttctggaa
     4501 aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc acgccggatg
     4561 cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt gcaggagtcg
     4621 aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa cccgcctctt
     4681 ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag tcaacagtgg
     4741 tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat gaatatcccg
     4801 aacatttaca ttcattcctt aatgacttac ccacccgacc aggggtgtat ctgtttcatg
     4861 gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc agtcgggtgc
     4921 tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg cgaattagct
     4981 ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta atcaaagaac
     5041 aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc ctgcaattaa
     5101 atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga gcccccaacc
     5161 tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc atcgctgatg
     5221 aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt cgtgcatgtt
     5281 ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc catgaggaac
     5341 atgcgctacg cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg ccttggcaag
     5401 gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt attcaaaact
     5461 ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt cggacacccg
     5521 ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc ggtaactatg
     5581 aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac tgaacaacag
     5641 tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc acgcgccctg
     5701 gtcggtagtg agcagtttgt cgcccagttg ccacgccccg ttaatcacaa acaccacccc
     5761 gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg ccttacagac
     5821 atccaggcgc gtcataatat tgaaatccat cgacatctgc cctgccgtca gtttcgcttt
     5881 taccacctgg tccgccgtaa aggcaaaagg ctgtaacggt tttaaggtat ggttaaagcg
     5941 gtctgcgctt tcaaggagca tctcgccgcc ttccagcaac gtcactatcc tttccatgcc
     6001 gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcac gccagtaaaa
     6061 atcacgtttt gccggtggga aagtggaaat ttcgcgcgtt tcaccggcag cgtttcgcca
     6121 cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg gcccgttctt
     6181 ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg atttacgtta
     6241 gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg ccgatcctgt
     6301 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaag
     6361 attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc agacgcttct
     6421 caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa attttgttct
     6481 gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt gcgatctgcg
     6541 cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt gcgcggcgtt
     6601 cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg tggatctgct
     6661 gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc ggaccgaact
     6721 tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg gtgtctgcgg
     6781 catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt gcagttaatt
     6841 tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa gtagcgtgcc
     6901 gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa cggtaaaaca
     6961 atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa tttgatggag
     7021 agtgatgaga gaatattaca aaacgatgat tttgcagaga ttatgaagaa ctataccgga
     7081 tgactggtga taaataaagc aaataaccag gattaatctg tattaattta taagaaagca
     7141 acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt cgagcattaa
     7201 tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact ctacatcatg
     7261 ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct caaacggcat
     7321 aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc gagtaatttg
     7381 cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct caccagatag
     7441 cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt gcccaaaggg
     7501 caacgctttg ccaagttcca acgtacaggc cagcgcgcca aaatgtctga cgctgaaatg
     7561 ggtaaacgta ccaccaggct cctgatggaa caccagcgcc tccagccccg ccgcacccag
     7621 ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac cgaactgcgg
     7681 atgcaaggag ccacgaattg cggtatgtag atcaaggtgc caacgcacag attctttgcc
     7741 ctggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg tttctccgct
     7801 ttcagcaaat agctgccaac gaccgccaaa cattcggttc atatcgctat ggcaataacg
     7861 tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc aacgtaacgg
     7921 gatctcgctg tgagatatcg cgccaagcaa cgcgtccagc atttccacag gtgccgtctc
     7981 attaccgtgg atccccgcag aaatcaccag cgcgccttgc ggtggcgtta atggcgtcag
     8041 ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc gctcggtgat
     8101 aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc cctccctcgc
     8161 gctggaacgg ataaaccgaa ccgagattca gta
//
LOCUS       DADLTF010000162         8157 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.164, whole genome shotgun sequence.
ACCESSION   DADLTF010000162 DADLTF010000000
VERSION     DADLTF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.164"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(61..600)
                     /locus_tag="JN749_003530"
     CDS             complement(61..600)
                     /locus_tag="JN749_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7369804.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /locus_tag="JN749_003531"
     CDS             complement(665..1213)
                     /locus_tag="JN749_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7369805.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JN749_003532"
     CDS             complement(1694..2290)
                     /gene="fimE"
                     /locus_tag="JN749_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HAY7369806.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(2768..3369)
                     /gene="fimB"
                     /locus_tag="JN749_003533"
                     /pseudo
     CDS             complement(2768..3369)
                     /gene="fimB"
                     /locus_tag="JN749_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimB"
     gene            4823..5539
                     /gene="nanC"
                     /locus_tag="JN749_003534"
     CDS             4823..5539
                     /gene="nanC"
                     /locus_tag="JN749_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HAY7369807.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5559..6665
                     /locus_tag="JN749_003535"
     CDS             5559..6665
                     /locus_tag="JN749_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HAY7369808.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNRWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKEEYYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            6730..7710
                     /locus_tag="JN749_003536"
     CDS             6730..7710
                     /locus_tag="JN749_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HAY7369809.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2422 a         1743 c         1699 g         2293 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata cgtaacttat
     1621 ttatgatatg gacagtttgg ccccaattgt cttgtgttta tttgtttttt aactttatta
     1681 tcaataagtt aaatcaaacc tcttctcttt ttaatttttc gtttatgaga ttatttcttt
     1741 cccataatcc ggcaaaacga gcagcattac tggcggtata acgcacagta tggcgaatat
     1801 ttcgatgccc gagataatcc tgaattaaac gggtatctgc accacgctcc gccagttcat
     1861 aaccgcaagc atgccttaac atatgaggat gagtctgcgt tacggttcca gcttcaatac
     1921 cggcatcgcg aataatgcga taggcctgct ggcgagaaag ccgactcccg cggcgagaaa
     1981 taaatatagc gtcggtccgg tcagcgcctt tccagttagc acgttcctgg gtccagcgtt
     2041 ccacggcttc acgctcatca aaacgtaacg ggtgaacggt agaaaatccg ttcttcagtc
     2101 ggcgaatatt tattcgacct tcattaaggt caaggtcctg ataatgcaga tcaagcagtt
     2161 cactaatacg catcccatgc cgatatgcca acagaataag acaataatct ctggctcccg
     2221 ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc tttaccggta agataacgac
     2281 gtttactcac aatagtagta ctcctaactg agatatattt gaatgaatac ctataggaaa
     2341 cctcaatcgg tcaaaattag cccaatagat aaaaagataa ccacacacga agttatgtgg
     2401 ctatcagtca attacattaa acatcataca aaaataaaat atcactgatg tgacaacatc
     2461 tattttcatt gatttgattc aatataacaa tagattacag aggattaact tccagttact
     2521 taaaaaataa agaaaaaagt gtggaaacag gagaatttaa gtgatgtgta aacttatgaa
     2581 tgattttgtt tatttatttt gaagaacata aaccattaga ttttgtatgg gtatggttaa
     2641 cttaatgttt attatgataa ttaagaatca cacttacaat aacatcaatt aataaatggt
     2701 taatcatttt tgatatatcg taagaataat gtagttttta acaccatccc tggtatctca
     2761 actatctcta taaaacagca tgacgctgtc gtcctctggc tctatcccag atgccgtaaa
     2821 aacgccctgc attgctggcg gtataccaga cagtatgacg aatattgcga tgcccaagat
     2881 aatcctagat aagtcgcgta tctattccta tattcgccaa agcaaaacca cacgaatagc
     2941 gtaacatgtg cggatgaatc tccagtgaca acccggcatt accaccagaa gtcgagataa
     3001 tatggtaaaa ctgttgccga gaaagcggat tccccttacg tgataaaaat acccactcgc
     3061 tctcagcatg cggataagaa gtacggatac tcaaccagtt ttttaaagcc tgaacttctt
     3121 tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa tcgatggata tatatacact
     3181 ttgctttaag atcaatatcc gaaatcctca atcgacaaat ttcactcgcc cggaaaccat
     3241 gaataaaaca aagcaaaatc agacaataat tacgtgttgc atgaggccgg tatttgctgc
     3301 tttaaggagt gactcgattt cactatgggt aaggaagttc ctttttttgt tatcagcctt
     3361 attcctcatc gttttccctt ataattacag acgcgcacta gctgtgctgg gttagcaata
     3421 atccaacatt tttatcctga ttatgtttat aaaagcgaac gtttgcttaa taacttacgt
     3481 aagtggacca gttcttctga gcgaacttaa atggagtagc gatagttact tataataact
     3541 ttgccataca aacgccatgc tcttgcatgc tatgtacctt tatatattta tcaatctgat
     3601 acgaatctaa taataaccct catcattaat gcaactaatc ttatctatat atcatgtgat
     3661 atgttctgta acaagtaaaa tcaacataaa aaattcagca aaatttaata tttgttcaac
     3721 tagccgtctc aaatatgaca ttattagtta actgcatttg ccacaaaatg gctatttcat
     3781 ggaacgcagt aatttcttag tggctattat catgctaatt cttagatccc tagcgattat
     3841 tcctgcctgg ttcgtcagct taggccatga gattgttaca ttccttttta tcctggagat
     3901 atgggggaac gcaggcgacg ctttttcatc tacatccgtt ggtaaatata gctttggtca
     3961 ctacgactta agccttcctc aatcacagct attcgccggg cgatacggca aacaaactaa
     4021 gtgaaacatc atcctgaaaa caccaacatc aacaagcctc tccagatcga catcaggagt
     4081 gaccagttac aagccacaaa ccaagaaaca tatttcatga tgagaattat gctcagcctg
     4141 atggcgagta aacccttatt ttcaccactc tcactgagtt agcttgcaaa tctttctttg
     4201 tttagattct aaataaattt tttataaaac atatagttaa aagaaaatct ttagtttttg
     4261 aagaccaccg caagtgttcg tctggcttca tatggcatct tcttcttaga aaaagatcga
     4321 catattttgt gacgcgaatt gcaaatctgg ttttgttgta tggattgcgt gatttttgat
     4381 ctggtataac aggtataaag atgcaccacg atagtcaatg agactgggca tctcgtaatc
     4441 tatggcaaac atcacttcag ttctttcaca tcgggtgatg aaaatgcact tcagtctgaa
     4501 aggaatatga aaatgagatc aacagacact ctattttatg actctgggta aaatgggttg
     4561 agtaagtgat ataacttacg aatattcaaa tcaattaaaa atcagaagag attttatact
     4621 caggtattta atctggatct ctgtttattt aaataatgtt aaaagagaat tttcacagga
     4681 gaccttatac aaaaaatata aaatacagtt acccgttgcc aaagacacta taagcctggc
     4741 aaaaaaatat tacacaacat aaatgctaat tgtttatgcg ggctttgtat tgctttctgt
     4801 atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata ctttctggcg tattattact
     4861 gtgcttttcg tccccattaa tttctcaggc tgcgacactg gacgtacgtg gtggatatcg
     4921 tagtggaagc cacgcctatg agactcgact caaagtcagt gagggatggc aaaatggatg
     4981 gtgggcaagc atggaaagta atacctggaa taccattcat gataataaaa aggaaaatgc
     5041 cgcactcaat gatgttcagg ttgaagttaa ttacgcaatt aaacttgatg atcaatggac
     5101 ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc acacgctacg gaccctacgt
     5161 aaaactgtcc tgggacgcga caaaagatct taattttggc attcgctatc gttacgactg
     5221 gaaagcttac cgacaacaag acttatccgg tgatatgtct cgtgataacg ttcatcgttg
     5281 ggatggatat gtcacttacc atattaatag tgatttcacc ttcgcatggc aaacgacgct
     5341 atacagcaaa cagaatgatt atcgctatgc aaaccacaag aaatgggcga cggaaaatgc
     5401 ctttgttcta caataccata tgacgcccga tattacgcca tacatagaat atgactacct
     5461 tgaccgccag ggtgtttaca acggcagaga taatttatcg gaaaacagtt atcgcattgg
     5521 tgtgtcattt aaactgtagt agacaggaga cagtcacaat gaataaaact ataatggcgc
     5581 ttgctatcat gatggcttca tttgccgcaa acgcgtctgt attaccggaa actcctgtgc
     5641 catttaaaag tggtaccgga gcaattgata acgacactgt ctacattggt ttaggtagcg
     5701 caggtacagc atggtacaag ctagatacac aggccaaaga taaaaaatgg actgcgttag
     5761 ctgcattccc tggtggaccg agagagcaag caacctctgc atttattgat ggcaatctgt
     5821 atgtgtttgg cggcattggc aaaaacagcg agggattgac tcaggtattt aatgacgtac
     5881 acaaatacaa ccccaaaacc aatcgctggg ttaaattgat gtcgcacgcg ccgatgggca
     5941 tggcgggtca tgtgactttt gtacacaacg gcaaggctta tgttactggc ggtgttaacc
     6001 agaatatctt caatggctat tttgaagatc tcaacgaggc tggaaaagat tcaaccgcta
     6061 tagataaaat caacgcccac tattttgaca aaaaagaaga atattatttc ttcaataagt
     6121 ttctgttgtc ttttgatccc tcaacacagc aatggagtta cgctggcgaa tcgccctggt
     6181 acggaacggc tggtgcggcg gttgtgaata aaggtgataa aacctggctt attaatggcg
     6241 aagccaaacc aggattgcga acggatgccg tatttgaact tgatttcacc ggtaataatt
     6301 taaaatggaa taagcttgat cccgtctcat caccagatgg cgtcgctggc ggttttgcgg
     6361 ggataagcaa tgattctctt atatttgccg gaggggccgg attcaaaggt tcacgagaaa
     6421 attaccagaa cggtaagaac tatgcgcatg aaggcctgaa aaaatcatat agcactgata
     6481 ttcatctttg gcataacggg aaatgggata aatcgggtga attatcgcaa ggtcgggcct
     6541 acggagtatc attgccctgg aataatagtt tattaattat tggcggtgaa actgcaggcg
     6601 gcaaagcggt gacggattca gttttaattt ctgtgaagga taataaagtc acagtacaaa
     6661 actaacgctt cagggccccg gtaaaggggc cctgctatca atttgtcatt tgtattacgc
     6721 gaaaattata tgaacgcaat aatatcgccc gattattact atgttcttac cgttgctggt
     6781 cagtctaatg ccatggcgta tggcgaagga ctgccattac cggacaggga agatgcgcct
     6841 cattccagaa ttaaacaatt agcgagattt gcgcatacgc atcccggagg cccgccatgt
     6901 cactttaacg acattattcc actgactcac tgcccacacg atgttcagga tatgcagggt
     6961 tatcaccatc ctctggcaac gaatcatcaa acacagtacg gcaccgttgg ccaggcactg
     7021 catattgcac ggaaattact gcccttcatt cctgataatg cagggattct tatcgttccg
     7081 tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag ggacatattc agaacggcac
     7141 ggagccagcc atgatgcttg tcgttgggga acggatactc cgctgtacca ggatttagtc
     7201 agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca aattcctcgg cgtatgctgg
     7261 atgcaaggcg aatttgactt aatgaccagt gactacgcgt cacaccctca acactttaat
     7321 catatggttg aagcctttcg tagggatcta aaacaatacc attctcagct taataatatt
     7381 actgacgcgc cgtggttttg cggcgatacc acctggtact ggaaagaaaa tttcccgcat
     7441 tcgtatgaag ctatttatgg caattatcaa aataatgttt tagccaatat tattttcgtc
     7501 gacttccagc aacaaggtga aagaggactg acgaacgcgc ctgatgaaga cccggacgat
     7561 ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg agaactggac gacggcactg
     7621 cgaagcagtc atttcagcac ggcagcccgt cgggggatta tttctgacag atttgtagaa
     7681 gcaattttgc agttttggcg cgaaaggtga gcggggagtt tattcatggg ggatgtcctt
     7741 gttcgttctc agagtagaca acaaaaaaca aatgagatca aggtaatatc tcttcacttg
     7801 cgggctcgaa cagcaggcaa ttgccaatat aataatctgg cacaagggtg aaaaacgcgc
     7861 accgcataaa ccgttgttat tgcttacaga ttagccggat gccttaatag acatccgcgc
     7921 cttttcgatt atggctcgga aatctacgaa catctacaca gtttactgga acgttttggg
     7981 ccacagcgtt cgcagcaccg tcctgatatg ccgttctggc gattataagt cgacggattc
     8041 tggcaaatag acgatgatga tatgcttaat gatgttcaaa gaaattaggc cattattata
     8101 ttattgcttg cattttcttt cattcctaaa agcacccgcc attaatccca ggcgcat
//
LOCUS       DADLTF010000163         8142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.165, whole genome shotgun sequence.
ACCESSION   DADLTF010000163 DADLTF010000000
VERSION     DADLTF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.165"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..893)
                     /locus_tag="JN749_003537"
     CDS             complement(<1..893)
                     /locus_tag="JN749_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HAY7369810.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPS
                     SVNVLSELPGRTVPYEAAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            953..1231
                     /locus_tag="JN749_003538"
     CDS             953..1231
                     /locus_tag="JN749_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369811.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JN749_003539"
     CDS             complement(1232..1759)
                     /gene="gadE"
                     /locus_tag="JN749_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HAY7369812.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JN749_003540"
     CDS             complement(2558..3130)
                     /gene="hdeD"
                     /locus_tag="JN749_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HAY7369813.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3385..3717
                     /gene="hdeA"
                     /locus_tag="JN749_003541"
     CDS             3385..3717
                     /gene="hdeA"
                     /locus_tag="JN749_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HAY7369814.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3833..4159
                     /gene="hdeB"
                     /locus_tag="JN749_003542"
     CDS             3833..4159
                     /gene="hdeB"
                     /locus_tag="JN749_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HAY7369815.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4223..4869
                     /locus_tag="JN749_003543"
                     /pseudo
     CDS             4223..4869
                     /locus_tag="JN749_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4911..5441)
                     /locus_tag="JN749_003544"
     CDS             complement(4911..5441)
                     /locus_tag="JN749_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7369816.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JN749_003545"
     CDS             complement(5597..6163)
                     /gene="slp"
                     /locus_tag="JN749_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HAY7369817.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
     gene            complement(6410..7633)
                     /locus_tag="JN749_003546"
     CDS             complement(6410..7633)
                     /locus_tag="JN749_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369818.1"
                     /translation="MSIDFTLGIINTYHGDIYNCTTNTDNVKTPDTPKWPYDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
BASE COUNT         2366 a         1575 c         1703 g         2498 t
ORIGIN      
        1 cggctacctt catccactaa tgccgtgaca tacatgccag gcagcaagtc accatttggg
       61 ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catcaaccgt cgggtcggag
      121 aatttcagcg tgccggtctg gctgtaacgt ttaccatttt ccagattgag ctgtactggc
      181 gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat gcgtaagaaa
      241 tcttgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac ggtaaccagc
      301 gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt cccgctgacg
      361 cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg ttcaacagcc
      421 gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc ggtgtcgtaa
      481 tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa ggtgatgcgg
      541 gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt tagctcggcc
      601 tgtaaaggtg caggatcaat ctgatacagc gaatcgctct ggttcacttt atcgccttcg
      661 ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc agcttcataa
      721 ggaacggttc taccgggcaa ttcgctcaac acattgaccg aactagggga gagtgtgacg
      781 acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc gcaggcggtg
      841 agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt cattttagtc
      901 cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag atatgaggga
      961 tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca ggcacagtga
     1021 tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag ttcctgccag
     1081 cattcgggca acgaagggtt ctactggtgg atacacatac caggggaatc aagtcgtttt
     1141 agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc ctcgtcatgc
     1201 cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata cccagggtga
     1261 cgatgtcgct catacgtctt agtttcagct tgtacataat gttgaatttc tgaatcttta
     1321 atgtttgttg cgaaatattg agcgtcgacg tgatattgct ttcattttta tttttcagaa
     1381 tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc ttatggggca
     1441 agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg ctaacaaaat
     1501 aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca ataattctct
     1561 cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga ttgtgatagg
     1621 tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac agggaattga
     1681 tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga aaagaatctt
     1741 tcgtcatgag aaaaatcata acttgctcct tagccgttat tgtttcgaac atgtcatcct
     1801 tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc tataagaact
     1861 ttgcccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat ttatccatca
     1921 cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg taaatagtaa
     1981 acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa ccagtttgtg
     2041 atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg agtctccttg
     2101 tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga aattacctga
     2161 gtaaataacg catttatgtc ttcaggtttt tatatagcgt ctggagacac ggatatttat
     2221 gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt aaccgcccat
     2281 agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa aacattaaac
     2341 ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc catggagtga
     2401 aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt atttcaatga
     2461 tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg atcatagtca
     2521 catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta acgaacaaac
     2581 tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc agtgtggaaa
     2641 ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg acgatatcca
     2701 gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga taccaactca
     2761 tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca atggcaaaaa
     2821 tgcccagctc cggtgcacgg atgaagaaat agccgatcaa taaataggcg actgcgacga
     2881 ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac ccgacaataa
     2941 gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata tcgccagaga
     3001 cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca ataaactgga
     3061 ttgctctgcg atgctttttc agcatctcca gatcaaactt caaaattgtt gccttatcta
     3121 tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat caatctatgc
     3181 caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc agtatctgat
     3241 gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt ccatcaacat
     3301 gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgattcgtg acggctcttt
     3361 cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct tggtggtctg
     3421 cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga taacaaaaaa
     3481 ccggtcaact catggacctg tgaagatttc ctggctgtgg acgaatcctt ccagccaact
     3541 gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc ggttttagat
     3601 gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca ggataaacaa
     3661 gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga tatgtaattc
     3721 cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat cttctttaaa
     3781 agcagctatt cctcctgttc atatataatc tctatattga atgggttaca aaatgaatat
     3841 ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt cactggtgaa
     3901 cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc aggaatttat
     3961 tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg aagaaacagt
     4021 atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa ttcctaaagt
     4081 gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa atcaagcatc
     4141 taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg agccatcttg
     4201 ctgctccttt ttgcattttt atatgacagc agaattaatt atacgtctta tactcgcggc
     4261 aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag cagggttacg
     4321 cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga aatatggttt
     4381 tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg ctgctcaggt
     4441 ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc aaaatattgt
     4501 aggtctgacg acggcagcgg atatctgggt gaccgccgca taggtatggt tattggcagc
     4561 ggtatgtacg aactgggtat ttatggttca gtgatgacct tgttggtgct ggaagtcttc
     4621 catcaattaa ccttccgcct gatgaacaaa aattaccatt tacagctgac attagtgaac
     4681 ggtaatacgg tatcaatgct cgactggttt aagcagcaaa aaataaaaac tgatttagtt
     4741 tcgttgcagg aaaatgaaga tcatgaagtg gtcgccattg atatccagct tcatgccaca
     4801 acgttggtag aagacctgtt gcgattatta aaaggtatgg caggggtgaa aggcgtttca
     4861 attagctaat ctgtgtggca gcgtagccag actcaccgta agcctgaaat tcacaccaga
     4921 taatcaatat gctgatggcg taccagctca ttgatccgtt taagattgag gatcatcatg
     4981 atattgtgtt tatggcagta gaacgttttt agcgatattt tcagtttctc cagaatatct
     5041 tcagttgtca tttgattcgc aatgcagctc agaacctgat gttgcttgcg cgaaagtgac
     5101 acattcgctt caggcacaga gcagtaactg ccattgatga gtgcgacaaa atcaatgata
     5161 gtgatagtcc gcggaacaaa ggtcaccggt actttcccca gacagcgttt aatattaaag
     5221 ggggagaaaa caatgacatg gacaggtttt aatcgttcaa tttgagtgag aaaattagaa
     5281 tgaaatacat tattcattaa tgtatcaata atgacgaagg cattctgatg caacattacg
     5341 tcgatctctt cttcgttctg tatatgaacg acattacctt tactcagaat gtttttcatc
     5401 gcggtgaaga acatcgtatc cctggtaatt ataagaaaca taaccgactc ctggtgcgga
     5461 cattaacttt ataatcagtt tgccaaaggt aattaacagg acagggctta taataataat
     5521 agttagctta ctgtatatta attttatgta atacatgatg cagatgctga gaaagcatct
     5581 gcatctttcg gtggtgttat ttgaccagct caggtgttac ctgactcacc gcattggtgt
     5641 agtaaggcgc acccaagccc ggttccggcc agaatgcacc atagccgtaa tcccacaggt
     5701 tataggtggt attaaccact tctctcaaat gccacacctg gatgccctgc atattcactt
     5761 ccaggaagtt atacgggact ttattgataa agcctggttg ttcaccctga atggtaccga
     5821 ggatggtaac aaagtgatta cgatagttca ctggatcaag gaagccgctt tgtctggcga
     5881 gcagtcggcc ctgatagttg gcttcaatat caggcttcgc atagctatcc aacggtaata
     5941 cagcgatttc taacaacgta tccgttttgc cattgataac gttgataacc ttcccaccaa
     6001 agcgcgcttg ttgaccaaca tataaccccg gctggttatg aacagcaaca aaactttttt
     6061 gaatatcagg ttggttattg cctttgatat tttgcggaat tgaactacat gcggcaagca
     6121 aaaatgaaag gctgaggatg agtgcacctt ttgtcatgtt catgttacta tccttatcaa
     6181 caaatattaa aaccataata gatatttctt tatagttttc atctgattct gaggtgatat
     6241 gcctgatagt aacgtaaacc tgtctgatgt ttatatccct tctgtttagt tcaattccgt
     6301 tattttgggg cgtggtatga gtatccatcg cccaataata taaccctttg ttttaaaatt
     6361 acataaaatc tatttttatt caatgattat aaagtgctaa taatcaatat tatggtatta
     6421 tattctgata atgatttgat aagcgaattc ttttgccaga tggtatttct atttccaccc
     6481 cataatttat atctttttca ttaggcagat ctctctttaa ataaaagggg tatgaaatct
     6541 tttcgggttg aagtgtctcg taatgaaatg ctatgaattc atttttagac attgttgccc
     6601 atttttgaat tttattgtat agagagggtg ttgtaaactc ttgaagttgt ttataacata
     6661 tttttttgaa ctcttgctta tattttaata tgtcatcaaa tgaaagattc actacctttg
     6721 cctctaagaa atcaattatt ctcgaagcaa tttccgcttt cgcgcgtaaa gagttgtcat
     6781 taatagttaa caatgtagaa aacaagccat tgatttgttt gtcaagatgt tgttcatatt
     6841 tatgtagttt ggggatggga ttttccttgt gtgaaaaaat agtgttaaat aaatttgcgt
     6901 cagtgaaatt tatattaagt agatcttcaa aataaaattt tgataaggtt aggactagct
     6961 tggacatatc ttgccctgtc atgttgaaat caataaagtc gcatccagca agggcttcca
     7021 tcctattttc atttttactg tgctcaatat acagcttcgc catttttaag tacaagcttt
     7081 gaatgtctct tatatcggtt ggtaagtcag ctcgttgccc agcaaaattt ttattatcgg
     7141 aagaaacggt ttggacactg aatctttcgg gtattccttc ttctacgaga attttagtta
     7201 tgattggatt gttgggttct ggattaacga caacaatgat ttgacataca gaatccttat
     7261 ccttttcgta tctaatgtta tgaatctgat taagtattga ttgtgtctgt tgcgtaagtg
     7321 gtgtcgaact tatcgtttca atgttattaa taagacttac aagttgaacc acaacttttg
     7381 agtagtctgc atttgtatat gtggtgttcg tgtggcatgc attaatttgt tggagttgtt
     7441 gttgcgctaa atcaaattgt tcagggttgt atccttctcc agagaatgtg gaattaatgg
     7501 gttgttgttc ttcccaatta tcataaggcc acttcggtgt gtctggcgtt ttaacattat
     7561 cggtatttgt tgtgcagtta taaatatcgc cgtgatatgt atttattata cctagggtaa
     7621 agtcaattga cataacattc cttattcatc atctgtttta tgctaatgtt tttaaattgg
     7681 ataattagaa caggaatgga ttttcttcta cacacaataa atataatgcg ttatatcttc
     7741 ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa
     7801 aagggcagtg atagtatcgt aaaggaaaac gttaacaact atcaaaaagc gctcatttta
     7861 aaaagttatt atgttctccg atagtgtgtg aagtaatatt tttgtgatgt tttagatgtg
     7921 taataatgaa ccaggttaaa gtgtatttaa aaatgtgttg tattagcgac tgaaatctgc
     7981 cttttaaaaa ggaggagggt actgagacaa agagtcattg cttctccgat aaaatgtcag
     8041 agaagcatga ttacgtggtt attgtctgtt gatgacttgt tgaccgagcc gtcatcattc
     8101 tcaatgcatt agttaaatca tttgtgattt ttaaggatta tc
//
LOCUS       DADLTF010000164         8079 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.166, whole genome shotgun sequence.
ACCESSION   DADLTF010000164 DADLTF010000000
VERSION     DADLTF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.166"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1242)
                     /locus_tag="JN749_003547"
     CDS             complement(<1..1242)
                     /locus_tag="JN749_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAY7369819.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRNAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="JN749_003548"
     CDS             complement(1239..2117)
                     /locus_tag="JN749_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7369820.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="JN749_003549"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="JN749_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="JN749_003550"
     CDS             complement(3022..3255)
                     /locus_tag="JN749_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369821.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4109)
                     /locus_tag="JN749_003551"
     CDS             complement(3252..4109)
                     /locus_tag="JN749_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005109065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY7369822.1"
                     /translation="MSAQYIDSAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSC
                     HEHCYAQIMVGRAGASQDAPGSMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTL
                     PTLSQPEIAIVDGQAVTSSLAVANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILV
                     RQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADM
                     SSVAQNARGVYLHLREIHQIWTSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRL
                     NGSDKEVLP"
     gene            4134..4463
                     /locus_tag="JN749_003552"
     CDS             4134..4463
                     /locus_tag="JN749_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAY7369823.1"
                     /translation="MNYTIEYYSEEVRLEVDQLPLSMRARYQHLVERMKVYGSNLGEP
                     HTSAFGDGLFELRIKGNDGIARVFYCTLTGKRIIMLHSFVKKTQKTPPAERKKAETRM
                     KEVKHDW"
     gene            4453..4761
                     /locus_tag="JN749_003553"
     CDS             4453..4761
                     /locus_tag="JN749_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7369824.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="JN749_003554"
     CDS             complement(4803..4979)
                     /locus_tag="JN749_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HAY7369825.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="JN749_003555"
     CDS             complement(4976..5386)
                     /locus_tag="JN749_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HAY7369826.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="JN749_003556"
     CDS             complement(5383..5661)
                     /locus_tag="JN749_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369827.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..>8079)
                     /locus_tag="JN749_003557"
     CDS             complement(5736..>8079)
                     /locus_tag="JN749_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HAY7369828.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1921 a         2201 c         1852 g         2105 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcattgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcaga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctacc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gcttttgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc
     7981 gcatagctca tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc
     8041 agagcctgcc atccgaacag gcaaggttcg caatcacct
//
LOCUS       DADLTF010000165         7860 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.167, whole genome shotgun sequence.
ACCESSION   DADLTF010000165 DADLTF010000000
VERSION     DADLTF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.167"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..167
                     /locus_tag="JN749_003558"
     CDS             <1..167
                     /locus_tag="JN749_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369829.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            350..2530
                     /locus_tag="JN749_003559"
     CDS             350..2530
                     /locus_tag="JN749_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HAY7369830.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTCNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2671..3954)
                     /locus_tag="JN749_003560"
     CDS             complement(2671..3954)
                     /locus_tag="JN749_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HAY7369831.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4089..5159)
                     /locus_tag="JN749_003561"
     CDS             complement(4089..5159)
                     /locus_tag="JN749_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7369832.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5137..5355)
                     /locus_tag="JN749_003562"
     CDS             complement(5137..5355)
                     /locus_tag="JN749_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAY7369833.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            complement(5440..6063)
                     /locus_tag="JN749_003563"
     CDS             complement(5440..6063)
                     /locus_tag="JN749_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HAY7369834.1"
                     /translation="MTSQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            complement(6385..7092)
                     /locus_tag="JN749_003564"
     CDS             complement(6385..7092)
                     /locus_tag="JN749_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7369835.1"
                     /translation="MAQLYGTDVVRIRQNHHENKSRFVEGVHFYKVVGDELRALKHKV
                     ALNYFVKSAPNARHLILWTERGAARHAKMLETDQAWDVFEKLEDGYFNQREKVANHPQ
                     PVPQPESSLDRARAMEIALNVADRLVNMFPNLSSESKQAAVAGVFNPIVGRDVVPLPA
                     ISEHYYSATEVGQRYGISANKVGRIANTYMLKTEKYGKWFIDKSAHNDKQVETFRYNE
                     AGVHKIEELIEGERKAA"
     gene            complement(7040..7474)
                     /locus_tag="JN749_003565"
     CDS             complement(7040..7474)
                     /locus_tag="JN749_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7369836.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIGAVVRNGCCAHPAKPS"
     gene            complement(7547..>7860)
                     /locus_tag="JN749_003566"
     CDS             complement(7547..>7860)
                     /locus_tag="JN749_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7369837.1"
                     /translation="QAYANAEFIAAFNPNVALALLDERERNLQYIKSRDQENEEIALT
                     VGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEASEKRIAELEAQTVTVKEVGD
                     A"
BASE COUNT         1811 a         1955 c         1929 g         2165 t
ORIGIN      
        1 ttactcacta tggcggcgcg gcaggtccgg tcatctttgg cgtgggttac aacgatatta
       61 aatcctggtg gttggtcggt gcggtactga cgatattaac cttcttggtg catatcaccc
      121 tcggcgtgtg gtggtggaat atgctgatcg gctggaacat gctgtaaata tactcgtcat
      181 acttcaagtt gcatgtgctg cgtctgcgtt cgctcacccc agtcacttac ttatgtaagc
      241 tcctggggat tcactcactt gtcgccttcc tgcaactcga attatttaga gtatatccat
      301 ttattatctt tctgcgcact tcacggtgcg cagatatctg gagcatttga tgatcaagtt
      361 atctgaaaaa ggcgtgtttc tcgccagtaa taacgaaata attgccgaag aacatttcac
      421 cggcgaaatt aaaaaagaag aagcaaaaaa aggcactatt gcctggtcta ttctctcttc
      481 tcataatacg tccggaaata tggataaact taaaattaag tttgattcat tagcctctca
      541 cgatattacc tttgttggta ttgtacagac cgctaaagcg tccggaatgg aacgtttccc
      601 gctgccgtat gtgctgacca actgccataa ctcactctgc gccgtcggcg gcaccattaa
      661 cggtgatgac catgtttttg gtttatcggc agctcagcgt tatggcggta tttttgtgcc
      721 tccgcatatt gcggtcatcc atcaatatat gcgtgagatg atggctggcg ggggcaaaat
      781 gatcctcggg tcagacagcc acacccgtta cggtgcatta gggacaatgg cagtcggtga
      841 gggtggcggt gagttggtaa aacagctgct taatgacacc tgggatatcg actatccggg
      901 cgtggttgcg gtgcatctga ccggaaaacc tgcgccgtat gtggggccac aggatgtggc
      961 gctggctatc attggcgcgg tgttcaaaaa cggttacgtc aaaaacaaag tcatggagtt
     1021 cgttggaccg ggcgttagcg cgctctctac cgatttccgt aacagcgttg acgtgatgac
     1081 cacggaaacc acctgtttaa gctccgtctg gcaaactgat gaagaagtgc ataactggct
     1141 ggcgctgcac ggtcgcggcc aggattactg ccagcttaac cctcaaccga tggcgtacta
     1201 cgatggctgc atcagcgttg atttaagcgc catcaaacca atgattgcgc tgccgttcca
     1261 cccgagcaac gtgtatgaaa tcgacacact gaaccagaac ctgaccgaca ttctgcgtga
     1321 gattgaaatt gagtccgaac gcgtggcgca cggtaaagcc aaactctcgc tgctggataa
     1381 agtggaaaat ggtcgcctga aagtgcagca ggggattatc gcgggctgtt ctggcggtaa
     1441 ctacgaaaac gtcatcgcgg cggcgaatgc actgcgcggt caatcctgtg gcaatgacac
     1501 cttctcgctg gcggtttacc cgtcatcaca gccggtgttt atggatctag ccaaaaaagg
     1561 tgtggtagca gatttgattg gcgcaggcgc aatcatcaga accgcattct gtggcccatg
     1621 ctttggcgcg ggcgatacgc caatcaacaa cggtttgagt attcgccaca ccacgtgtaa
     1681 cttcccgaac cgcgaaggct ctaagccagc caatgggcag atgtcagcgg tggcgctgat
     1741 ggacgctcgt tctatcgctg cgactgcggc aaacggtggc tatttaacct ctgccagcga
     1801 acttgattgc tgggacaacg tgccggagta cgccttcgat gtaacgccgt ataaaaaccg
     1861 tgtttatcag ggctttgtga aaggggcaac tcagcaaccg ctgatttacg gaccgaacat
     1921 taaagactgg ccggaattgg gtgcgctgac tgacaatatc gtcctgaaag tgtgctcgaa
     1981 gatcctcgac gaagtcacca ccaccgacga actgattcct tccggtgaaa cctcttctta
     2041 tcgttcaaat ccgattggtc tggcggggaa tgtcagcgag ctgacagagg tgtttgcgcg
     2101 cattaagcag attgctggtc aggagcatat tgatccgctg caaactgaaa ttggcagcat
     2161 ggtctatgcg gtgaaaccag gcgatggttc tgcgcgtgaa caggcggcga gctgccagcg
     2221 tgtgattggc ggtctggcga atattgccga ggagtacgcg actaaacgct accgttctaa
     2281 cgtcatcaac tgggggatgt taccgctgca gatggcggaa gtgccaacct ttgaagtggg
     2341 ggattacatt tacatccctg gcattaaagc ggcgctggat aatccgggta cgacgtttaa
     2401 aggttatgtg atccatgaag atgcgccggt aacggaaatt acgctttaca tggaaagcct
     2461 gacggctgaa gagcgcgaga ttatcaaggc gggtagtttg attaacttca ataaaaacca
     2521 tcagatgtaa aaagcgccat gtgaatgtag gtcgcattcg gcacttattg tcggatgcga
     2581 tgctggcgca tcttatccga cctacaaatc gcatcgaacc gtaggccgga taaggcgttt
     2641 acgccgcatc cggcaaatag ttaatagctc ttacttcttc gcctctgcaa ccactttgct
     2701 gcccacgccg cggttattgt attcccacat gcggttgtag ttagtgtcat tcagattgcg
     2761 ctgtatttcg tcgttatcat ctacgctgcc ggtattaccc gcaaacggac gattagagat
     2821 caccgcatcg gcccacggtt tagccgtgtt aaaaccttcg ttgatggcgc tatcacggat
     2881 caccacctga ccgttggtat tagcatcaac atccagcgag cggcccagtt gcgccacgcc
     2941 atcaccggaa gcattgaaac ggctgtttac ggcgaggaaa ccgtaataga tgttagacag
     3001 cgtagccggt gcaaacacat atgcttcttg ctgggtacgt gagttcacca cgcggaattc
     3061 ggtgttatcg aacaccactg cgccgcgacc agaaacgata tccacatccc cttcaatata
     3121 gctgttggtc accagcgtac gcggctgacg gttggtttcc agacggttct gcacaccgct
     3181 gttggtgaca aagaaggtgt tctgacgacc gagaatgtta acgttgttaa tctgtacctg
     3241 gtcaccgtca gtacgcagtg ctaccgccgg atggttacct gcatctacac tatcgcccag
     3301 cgtgttttcg atggtcagat tttgcagttg cagtccattg ttttgtgacc agaagaccgc
     3361 agaacacaga acaccgatac tgtcgctgcg tttgctctgg cagctatcgt acatatacca
     3421 cgctggttta cctggcatat atttgccgcg cgggttgacg tcgtgacgcc agtcggcagg
     3481 gctcatgccc ccatcaaggg aaagcccaat cttcacatca atcggttttt cacctgtacc
     3541 gtacagagtg attccacctg gagcagcagg gacatacacc gttccctgat actcaccagg
     3601 catcacggca atatactggc gcttgttggt gcgcttgata attgccgcat ctaccgccgc
     3661 ctgaatcgtg gtatgcgtta caccttgagt acccgccggg ccgacaacaa agtcaggttg
     3721 cgcaggcagg gttatcgggg acggattcca cgctgcagca cctggtgtca gggatgcaaa
     3781 atagtgttgc gcatcgaaat tcagcgcttc ttttgccgac agaatcgggc gagaagaggt
     3841 accaggcgca gtttgatcag aaggacgttg atcgggtggt gttgagctac aggcggtcag
     3901 cgtcacgcca aaagccaatg ccagcgccag acgggaaact gaaaatgtgt tcacaggttg
     3961 ctccgggcta tgaaatagaa aaatgaatcc gttgaagcct gcttttttat actaagttgg
     4021 cattataaaa aagcattgct tatcaatttg ttgcaacgaa caggtcatta tcagtcaaaa
     4081 taaaatcatt atttgatttc aattttgtcc cactccctgc ctctgtcatc acgatactgt
     4141 gatgccatgg tgtccgactt gtgcccgaga agatgttgag caaacttatc gcttatctgc
     4201 ttctcataga gtcttgcaga caaactgcgc aactcgtgaa aggtaggtgg atccccttcg
     4261 aaggaaagac ctgatgcttt tcgtgcgcgc ataaaatacc ttgatactgt gccggatgaa
     4321 agcggttcac gacgagtaga tgcaattatg gtttctccgc caagaatctc tttgcattta
     4381 tcaagtgttt ccttcattga tatcccgaga gcatcaacat gcaatgttgt agggatggca
     4441 atttttacgc ctgttttgct ttgctcgaca taaagatatc catctacgat atcagaccac
     4501 ttcatttcgc ataaatcacc aactcgttgc ccggtaacaa cagccagttc cattgcaagt
     4561 ctgagccaac atggtgatga ttctgctgct tgataaattt tcaggtattc gtcagccgta
     4621 agtcttgatc tccttacctc tgattttgct gcgcgagtgg cagcgaccgg gtttgttgtt
     4681 atatggcctt cagctattgc ctctcggaat gcatcgctca gtgttgatct gattaacttg
     4741 gctgatgccg ccttgccctc gtctatgtat ccattgagca ttgccgcaat ttcttttgtg
     4801 gtgatgtctt caagtggagc atcaggcagc cccctcctta ttgctttaat tttgctcatg
     4861 taatttatga gtgtcttctg cttgattcct ctgctggcga ggattttttc gtagcgatca
     4921 agccatgaat gtaacgtaac agaattatca ctgttgattc tcgctgtcag aggcttgtgt
     4981 ttgtgtcctg aaaataactc aatgttggcc tgtatagctt cagtgattgc gattcgcctg
     5041 tctcggccta atccaaactc tttacccgtc cttgggtccc tgtagcagta atatccattg
     5101 tttcttatat aaaggttagg gggtaaatcc cggcgctcat gacttcgcct tcttcccatt
     5161 tctgatcctc ttcaaaaggc tacctgttac tggtcgattt aagtcaacct ttaccgctga
     5221 ttcgtggaac aaatactctc ttccatcctt aaccggagga gggaatatcc tgcactcgcg
     5281 tacccatcga cgaactgttt caaggctcct tgggcgtcgc tggcgtgcgt tccactcctg
     5341 aagtgtcaag tacatcgcaa agtctccgca attactcgca agggagcgag tattggttta
     5401 ttttctccaa caaaaaagga gccgaagctc ctttgatgat taaaattcga attgtctcgc
     5461 ccgaaggctt ttcagcattg gttttgcccg ttcgaaaagg gcgcttgctt ggtcaagtcg
     5521 tgtcgcctcc cttagcagta catctctgtt tttcgtcacc gtgtaaaaag tttcaaacgc
     5581 gatgtcatac agcttgctcg tgtatgatga gttcagttcc ttcaatatcg ggtacagacg
     5641 tttgcagatg tcctgcgcat tctccatctg tacctgcata tagcaaagga ggatgatttc
     5701 ttcgtctgtg aattgctgcg ctggttgcat gctgcgaagt ttcttttcgc actcgatgaa
     5761 gtatcggcgt atctggcggc ctttttcgtt acgctcgacc atcgccgttt ctttggctgt
     5821 atcgagggtg aggtgatagt ctttgctacg gcgatcgcct ccacgtcctt tgatccccct
     5881 gttttggtga accaaaatgt aatcgacatc aacggcaaag ccgtattctg ttatgcgatt
     5941 tttaatccat gtagagaagt cacgacgtac ttccaaaaat tcgtgtaaat cacgggcatt
     6001 aacgagaaga gttgtttcgt tggatatagt gccgttgaat acggggatga gttgactggt
     6061 catttttatg tcctttgcat ttgttcagat agccctgtgt tcagcagggc ggtcaggtac
     6121 ttgaacaccg tgcaaagtcg gcccgtatcc ttaacctttc ggctgttttt cggtatacgc
     6181 gctacccgac catatctgaa aaatggacat aaaaaatccg catgactgac gggtgcggtt
     6241 tccgctttgc aaggtgtgtt cagcaccatg aagcggaata tagccccgtt aatgcggtgt
     6301 tgtcaaatca tgtaggcctt atcttgctgt aagccgcgcc attcgggctt ttccccacat
     6361 ttggggaaaa ctatcttgtc aaaatcatgc agcctttcgc tctccttcga tgagttcctc
     6421 tattttgtgc actccagcct cgttgtaccg aaatgtttca acctgcttgt cattgtgcgc
     6481 cgatttgtcg atgaaccact tcccgtactt ttctgttttg agcatgtagg tgttggctat
     6541 gcgtcctacc ttgttggctg aaataccgta gcgctggccg acttccgtag cagagtagta
     6601 gtgctctgag atagctggca gaggtacaac atcgcgacca acaatcgggt tgaacactcc
     6661 agctactgct gcctgctttg attctgagga cagattcggg aacatattca ccagtcgatc
     6721 cgccacgttc aaagctattt ccatggctcg cgcacggtct aggctacttt ctggttgcgg
     6781 tactggttga ggatggttgg caaccttttc gcgctgattg aagtagccgt cctccagttt
     6841 ttcgaacacg tcccatgcct gatccgtttc gagcatcttc gcgtgacggg cggctccgcg
     6901 ctctgtccag aggatgaggt ggcgtgcatt tggggcactt ttcacaaagt agtttaaagc
     6961 tactttgtgt tttagcgcgc gcagctcgtc accaacgacc ttgtagaagt gtaccccctc
     7021 aacaaagcgc gacttgtttt catgatggtt ttgccggatg cgcacaacat ccgttccgta
     7081 caactgcgcc aatgtttggt gggtagttgt ttttaaagtc agctgctaac acatagcatt
     7141 gtcgctctcc catttctggc attcgctcac tacagcttat ccaggcatcc ggagttaccg
     7201 gcactggctg ggcggtataa agcggtgtta tatctgcccg aaaattacat gctttatgca
     7261 gccgcaccca ccgttcgact tctgctttgt cagaatacat accagtgaac gtgttatatt
     7321 cacggtcaat ttgcgtgaag gttaccttcc acgccaccgg ctctgctgtc agcgatgcca
     7381 gagcaatttc ataagcacgg cgttcaacat tgtctcgcac atccaaactg ctgatacgct
     7441 ctttgatttc tttaatcagt tctttgtcgt tcacttatgc ctccgattcc cggatgtcac
     7501 ccaaatcaac gctacaatgc caaccagtgc aacgagcgca aaactatcaa gcatctccaa
     7561 cctctttaac ggtaactgtc tgtgcctcca gttctgctat gcgcttttct gaggcttcaa
     7621 gtaacgcctg cttatcgcgt agcgcttctt ccagttcagc aacatggcat tcactatcaa
     7681 taaggttgtt ctctgctgct tccagctcaa cacgcaactt ccctaccgtt agcgcaattt
     7741 cctcgttctc ctgatcgcgg cttttgatgt attgcaggtt tctttcccgt tcatccagta
     7801 gtgccagcgc aacatttgga ttaaaagcag caataaattc agcgtttgca taagcctgaa
//
LOCUS       DADLTF010000166         7859 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.168, whole genome shotgun sequence.
ACCESSION   DADLTF010000166 DADLTF010000000
VERSION     DADLTF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.168"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            81..408
                     /locus_tag="JN749_003567"
                     /pseudo
     CDS             81..408
                     /locus_tag="JN749_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="JN749_003568"
     CDS             complement(438..3350)
                     /locus_tag="JN749_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369838.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3353..3469
                     /locus_tag="JN749_003569"
     CDS             3353..3469
                     /locus_tag="JN749_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7369839.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3833..4675
                     /locus_tag="JN749_003570"
     CDS             3833..4675
                     /locus_tag="JN749_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7369840.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="JN749_003571"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="JN749_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7369841.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNEPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="JN749_003572"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="JN749_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7369842.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="JN749_003573"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="JN749_003573"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HAY7369843.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2116 a         1762 c         1861 g         2120 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc tgccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaat
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgtgta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctgt cgtccagcaa ccggatgctc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgcc ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agcacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tactaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaagcat cttcggcagg acttgttgag
     4381 cgggagagaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacacc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattggga attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aacaaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaacc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aatcaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatgt tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaatccgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DADLTF010000167         7833 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.169, whole genome shotgun sequence.
ACCESSION   DADLTF010000167 DADLTF010000000
VERSION     DADLTF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.169"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(194..1447)
                     /locus_tag="JN749_003574"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="JN749_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="JN749_003575"
     CDS             1554..2447
                     /locus_tag="JN749_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7369844.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="JN749_003576"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="JN749_003576"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HAY7369845.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="JN749_003577"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="JN749_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7369846.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="JN749_003578"
     CDS             complement(4575..5867)
                     /gene="clcB"
                     /locus_tag="JN749_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HAY7369847.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="JN749_003579"
     CDS             complement(6026..6640)
                     /gene="dmsD"
                     /locus_tag="JN749_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HAY7369848.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6683..7537)
                     /locus_tag="JN749_003580"
     CDS             complement(6683..7537)
                     /locus_tag="JN749_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HAY7369849.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7539..>7833)
                     /locus_tag="JN749_003581"
     CDS             complement(7539..>7833)
                     /locus_tag="JN749_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369850.1"
                     /translation="CPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEF
                     GPIEELRQKHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
BASE COUNT         2073 a         1997 c         2051 g         1712 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 cccgcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 gcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcaaaaa ggccacgaaa ctggggggga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gtgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaatga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaattgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
     5221 tcgctatgat taatcagatt gctaaccagc aatgccacga cggcggaaat aatcaccggg
     5281 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
     5341 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
     5401 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
     5461 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
     5521 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
     5581 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
     5641 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc agggggaaag gtttgtcgct
     5701 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
     5761 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
     5821 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
     5881 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
     5941 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgcg cgtaatcgtc
     6001 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
     6061 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
     6121 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
     6181 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
     6241 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
     6301 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
     6361 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
     6421 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
     6481 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
     6541 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgctccc aacacgcgcg
     6601 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
     6661 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
     6721 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
     6781 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
     6841 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
     6901 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
     6961 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
     7021 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
     7081 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
     7141 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
     7201 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
     7261 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
     7321 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
     7381 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
     7441 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
     7501 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
     7561 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
     7621 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtacc gtgcttctgg
     7681 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
     7741 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
     7801 tcagcattgt actgtggtgc gccgtacgga caa
//
LOCUS       DADLTF010000168         7795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.170, whole genome shotgun sequence.
ACCESSION   DADLTF010000168 DADLTF010000000
VERSION     DADLTF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.170"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(337..627)
                     /locus_tag="JN749_003582"
     CDS             complement(337..627)
                     /locus_tag="JN749_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369851.1"
                     /translation="MDATGNTTVTCLSHKMKYKEVACGKQRMHWLALQFVDENNNPVS
                     GLNVQLEYHPRAMAAELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="JN749_003583"
     tRNA            complement(1227..1302)
                     /locus_tag="JN749_003583"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="JN749_003584"
     CDS             complement(1408..2115)
                     /locus_tag="JN749_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAY7369852.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            2513..4648
                     /locus_tag="JN749_003585"
     CDS             2513..4648
                     /locus_tag="JN749_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7369853.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LVIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVVMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /locus_tag="JN749_003586"
     CDS             complement(4697..5953)
                     /locus_tag="JN749_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HAY7369854.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JN749_003587"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="JN749_003587"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HAY7369855.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="JN749_003588"
     CDS             complement(7299..7574)
                     /locus_tag="JN749_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HAY7369856.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..>7795)
                     /locus_tag="JN749_003589"
     CDS             complement(7602..>7795)
                     /locus_tag="JN749_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7369857.1"
                     /translation="TAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSV
                     GLAAPVERLLQQLRTGAPV"
BASE COUNT         1908 a         1879 c         2007 g         2001 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgccggttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aagtagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggcattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggtgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggtga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtca
//
LOCUS       DADLTF010000169         7508 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.171, whole genome shotgun sequence.
ACCESSION   DADLTF010000169 DADLTF010000000
VERSION     DADLTF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.171"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="JN749_003590"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="JN749_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HAY7369858.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDDLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLCGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            1158..2117
                     /gene="rbsC"
                     /locus_tag="JN749_003591"
     CDS             1158..2117
                     /gene="rbsC"
                     /locus_tag="JN749_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HAY7369859.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIVGT
                     LIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2142..3019
                     /gene="rbsB"
                     /locus_tag="JN749_003592"
                     /pseudo
     CDS             2142..3019
                     /gene="rbsB"
                     /locus_tag="JN749_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3145..4074
                     /gene="rbsK"
                     /locus_tag="JN749_003593"
     CDS             3145..4074
                     /gene="rbsK"
                     /locus_tag="JN749_003593"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7369860.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQLLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4078..5070
                     /gene="rbsR"
                     /locus_tag="JN749_003594"
     CDS             4078..5070
                     /gene="rbsR"
                     /locus_tag="JN749_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HAY7369861.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5036..6463)
                     /locus_tag="JN749_003595"
     CDS             complement(5036..6463)
                     /locus_tag="JN749_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HAY7369862.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVTRLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6486..7178)
                     /locus_tag="JN749_003596"
     CDS             complement(6486..7178)
                     /locus_tag="JN749_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAY7369863.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1909 a         1816 c         1979 g         1804 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatgacc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctctgcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcatcgtc tattctcttt gtggtctgct ggcatcgctg gccgggatca
     1861 ttgaagtggc gcgtctctcc tctgcacaac ccacggcggg gactggctat gagctggatg
     1921 ctattgctgc ggtggttctg ggcggtacga gtctggcggg cggaaaaggt cgcattgttg
     1981 ggacgttgat cggcgcatta attcttggct tccttaataa tggattgaat ttgttaggtg
     2041 tttcctccta ttaccagatg atcgtcaaag cggtggtgat tttgctggcg gtgctggtag
     2101 acaacaaaaa gcagtaataa cgactacagg acatcttgaa tatgaacatg aaaaaactgg
     2161 ctaccctggt ttccgctgtt gcgctaagcg ccaccgtcag tgcgaatgcg atggcaaaag
     2221 acaccatcgc gctggtggtc tccacgctta acaacccgtt ctttgtatcg ctgaaagatg
     2281 gcgcgcagaa agaggcggat aaacttggct ataacctggt ggtgctggac tcccagaaca
     2341 acccggcgaa agagctggcg aacgtgcagg acttaaccgt tcgcggcacc aaaattctgc
     2401 tgattaaccc gaccgactcc gacgcagtgg gtaatgctgt gaagatggct aaccaggcga
     2461 acattccggt tatcactctt gaccgccagg caacgaaagg tgaagtggtg agccacattg
     2521 catctgataa cgtactgggc ggcaaaatcg ccggtgatta catcgcgaag aaagcgggtg
     2581 aaggtgccaa agttatcgag ctgcaaggca ttgccggtac atccgcagcc cgtgaacgtg
     2641 gcgaaggctt ccagcaggcc gttgctgctc acaagtttaa tgttcttgcc agccagcaga
     2701 ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
     2761 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcaaa ctgccggtaa
     2821 atcggatgtg atggtcgtcg gatttgacgg tacaccggat agcgaaaaag cggtgaatga
     2881 tggcaaacta gcagcgacta tcgctcagct acccgatcag attggcgcga aaggcgttga
     2941 aaccgcagat aaagtgctga aaggcgagaa agttcaggct aagtatccgg ttgatctgaa
     3001 actggttgtt aagcagtagt tttaatcagg ttgtatgacc tgatggtgac ataaatacgt
     3061 catcgacaga tgaacgtgta atataaagaa aagcagggca cgcgccaccc taacacggtg
     3121 gcgcatttta tggacatccc gaatatgcaa aacgcaggca gcctcgttgt tcttggcagc
     3181 attaatgctg accacattct taatcttcaa tcttttccta ctccaggcga aaccgtaacc
     3241 ggtaaccact atcaggttgc atttggcggc aaaggcgcga atcaggctgt ggctgctggg
     3301 cgtagcggtg cgaatatcgc gtttattgcc tgtactggcg atgacagcat tggcgagagc
     3361 gttcgccagc tgctcgccac tgataacatc gatattactc cggtcagcgt gatcaaaggc
     3421 gaatcaacag gtgtggcgct gatttttgtt aatggcgaag gtgagaatgt catcggtatt
     3481 catgccggcg ctaatgctgc tctttcccca tcgctggtgg aagcgcaacg tgagcgtatt
     3541 gccaacgcgt cagcgttatt aatgcagctg gaatcaccac tcgaaagtgt gatggcagcg
     3601 gcgaaaatcg cccatcaaaa taagactatc gttgcgctta acccggctcc ggctcgcgaa
     3661 cttcctgacg aactgctggc gctggtggac attattacgc caaacgaaac ggaagcagaa
     3721 aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag cgaaggcggc gcaggtactg
     3781 catgaaaagg gtatccgtac tgtactgatt actttaggaa gtcgtggtgt atgggctagc
     3841 gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc aggctgtcga taccattgct
     3901 gccggagata cctttaacgg cgcgttaatc acggcgttgc tggaagaaaa accattgcca
     3961 gaggcgattc ggtttgccca tgctgccgct gcgattgccg taacacgtaa aggcgcacaa
     4021 ccttccgtac cgtggcgtga agagatcgac gcatttttag acaggcagag gtgacgcttg
     4081 gctacaatga aagatgttgc ccgtttggcg ggcgtttcta cctcaacagt ttctcacgtt
     4141 atcaataaag atcgcttcgt cagtgaagcg attaccgcca aagttgaagc ggcgattaaa
     4201 gaacttaatt acgcgccatc agctctggcg cgtagcctca aactcaatca aacacatacc
     4261 attggcatgt tgatcactgc cagtaccaat cctttctatt cagaactggt gcgtggcgtt
     4321 gaacgcagct gcttcgaacg cggttatagt ctcgtccttt gcaataccga aggcgatgaa
     4381 cagcggatga atcgcaatct ggaaacgctg atgcaaaaac gcgttgatgg cttactgtta
     4441 ctgtgtaccg aaacgcatca gccttcgcgt gaaatcatgc aacgttatcc gacagtgcct
     4501 accgtgatga tggactgggc tccgttcgat ggcgacagcg atcttattca ggataactcg
     4561 ttgctgggcg gagacttagc aacgcaatat ctgatcgata aaggtcatac ccgtatcgcc
     4621 tgtattaccg gtccgctgga taaaactccg gcgcgcctgc ggttggaagg ttatcgggcg
     4681 gcgatgaaac gtgcgggtct caacattcct gatggctatg aagtcactgg tgattttgaa
     4741 tttaacggcg ggtttgacgc catgcgccaa ctgttatcac atccgctgcg tcctcaggcc
     4801 gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc aggcgttata tcaggcagag
     4861 ttacaggttc cgcaggatat cgcggtgatt ggctatgacg atatcgaact ggcaagcttt
     4921 atgacgccac cattaaccac tatccaccaa ccgaaagatg aactggggga gctggcgatt
     4981 gatgtactca tccatcggat aacccagccg acccttcagc aacaacgatt acaacttact
     5041 ccgattctga tggaacgcgg ttcggcatag atttacgctg tcttttgatc aaattattac
     5101 catcggttgt tttcagaagc atgaacattg ctgctgaagc aacagtaata atgcccatcg
     5161 tgataaacgt atagtggaat tgttcgacag tcgttgtgcc ttccatccct tcataaatgc
     5221 gaaggacggc cgcacttaca gcaacgccta aactaatcga cagttgctgc gtgaccgcca
     5281 gaacattgtt accgctgctg gcgttgtcat cggtcagatc ggcaagtgtg atggtattca
     5341 tcgcggtaaa ctgcgtcgac atagccatcc ctaatataaa caacggcaag atcagcatcc
     5401 atatcgccat tgccggtgat tgcaaagaga actgagcgat cattagccca ataatcaccg
     5461 tgatccccac taatgtatgg cgatagccca gacgacgtaa gacttgggta accatcgatt
     5521 ttgcaataat ggaacctaac gctgtcggtg ccatcataca gccggcaata aacgcctgat
     5581 aaccaaatcc tacctgtaac atcaatggca taaggaacgg tacacagccg gtccccagac
     5641 gggttgcaat attgcctacg ataccgatcg agaaagtgcg ggttttaaat aaatctaatg
     5701 aaattaatgg gtttggcgta cgtcgcgcat ggagaatata gagaagcagt aacccgatgc
     5761 tggtgacaat taccgtcaag gcaatccagc tggcgacaat cttttccccg aatagctcta
     5821 ttccgcttga aaagagaaca aggctgaggc caaacagcaa aaagccagtg atatcgaatc
     5881 tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc gtaaagaagg cccgcaatac
     5941 ctatggggat attaattaaa aatatccagt gccaggttgc ccaggtgacc agcacgccgc
     6001 caagaacggg gcctaaaatt ggccccacca gacccggcat ggcgacaaaa ttcaataccg
     6061 gaagaagttc attacgagga taagcacgca gtaaggccag ccgagtaaca ggcatcatca
     6121 ttgcgtcgcc tatcccttga ataacccgga agacaaccag ctgtggtagc gaattagaaa
     6181 gtgcgcaggc cagagaaccc aacgtgaaca gactcacggc aagggtaaaa atgcgacgcg
     6241 taccgaagcg atcggctagc catccgctta ccggaataag catcgccacc gtcagcgtat
     6301 aactgatgat ggctgattgc atcgcgagag gagaacgatt aaggctatga gcgattgcgg
     6361 gtaaggcggt attcagaata gtggcatcaa gtgcctgcat gaaaaaggcc atcgccgcga
     6421 tccacggcaa acccgacata ctacgcttct ttttatcgct cattcaatgt cctgttatcg
     6481 ggttatcact tatcaggtga gcgtagcagc gcctgacaag ctttaaatgc cgcgtcgcca
     6541 tcgctttgga taatcgcatc gacaatcgcc tggtgcagat ccagctttat cactgtgtcg
     6601 ctggtaattg acgtgaagta agtgtgataa accgaatgga atagcgaggc gaatgaggtc
     6661 aaaaacggat tggcgctcat ttcatagata tgctcatgcc aggctatatc gacttcgatc
     6721 cagcgttcac ggcgaaagtt ctcttttaat gccgccattt cggccattaa cgtattgaga
     6781 tgcgccttct gttccgcggt gccaaccgtt gctgccagta ggcaggcttg cggctccaga
     6841 cagatacgca taaccagaaa gtgatcgatg acctgatgaa agttctcttc tgtcatccac
     6901 caagtaagca attcctgatc aagaaaattc cagttcgatt gtggcatgac ccgagtacca
     6961 attcgcggtc gcggtaaaac catccctttt gccgttaacg ttttgaccgc ttcgcgtacc
     7021 gctgtacgac tcactccaaa ttgctcgccc agctcaatct caccgggcaa aatggtgccg
     7081 ggttcatatt caccttttaa gatccgttgc gccagcttct cagccagaac atacgaaagg
     7141 tttttctgtg cagctaactg ttgtgcgctt aaaggcatta cttatcttcc tttttctttt
     7201 tattcctcct tagtatgcca ccaggaagtg tgattacggt tgcaaaaacg gcaaattgat
     7261 tgttttatgg cacattaacg gggcttttgc tgaaaaaatg cgcggtcaga aaattatttt
     7321 aaatttcctc ttgtcaggcc ggaataactc cctataatgc gccaccactg acacggaaca
     7381 acggcaaaca cgccgccggg tcagcgggat tctcctgaga attccgtcag agaaagcaaa
     7441 aataaatgct tgactctgta gcgggaaagc gtattatgca cacctcgcgc cgctgagaaa
     7501 aagcgaag
//
LOCUS       DADLTF010000170         7471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.172, whole genome shotgun sequence.
ACCESSION   DADLTF010000170 DADLTF010000000
VERSION     DADLTF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.172"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            15..1073
                     /locus_tag="JN749_003597"
     CDS             15..1073
                     /locus_tag="JN749_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7369864.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1116..1619
                     /gene="yeaK"
                     /locus_tag="JN749_003598"
     CDS             1116..1619
                     /gene="yeaK"
                     /locus_tag="JN749_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HAY7369865.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVIHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1620..1724)
                     /locus_tag="JN749_003599"
     CDS             complement(1620..1724)
                     /locus_tag="JN749_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369866.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1894..2340
                     /locus_tag="JN749_003600"
     CDS             1894..2340
                     /locus_tag="JN749_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAY7369867.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2297..3118)
                     /locus_tag="JN749_003601"
     CDS             complement(2297..3118)
                     /locus_tag="JN749_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7369868.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3215..4396
                     /gene="nimT"
                     /locus_tag="JN749_003602"
                     /pseudo
     CDS             3215..4396
                     /gene="nimT"
                     /locus_tag="JN749_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4451..4798
                     /locus_tag="JN749_003603"
     CDS             4451..4798
                     /locus_tag="JN749_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HAY7369869.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="JN749_003604"
     CDS             complement(4820..5074)
                     /gene="yoaF"
                     /locus_tag="JN749_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HAY7369870.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5257..6282
                     /gene="dgcP"
                     /locus_tag="JN749_003605"
     CDS             5257..6282
                     /gene="dgcP"
                     /locus_tag="JN749_003605"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HAY7369871.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6315..6413
                     /gene="yoaK"
                     /locus_tag="JN749_003606"
     CDS             6315..6413
                     /gene="yoaK"
                     /locus_tag="JN749_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HAY7369872.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6416..6490
                     /gene="yoaJ"
                     /locus_tag="JN749_003607"
     CDS             6416..6490
                     /gene="yoaJ"
                     /locus_tag="JN749_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HAY7369873.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6542..6973)
                     /locus_tag="JN749_003608"
     CDS             complement(6542..6973)
                     /locus_tag="JN749_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369874.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKALEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(6967..7194)
                     /locus_tag="JN749_003609"
     CDS             complement(6967..7194)
                     /locus_tag="JN749_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7369875.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1834 a         1794 c         1805 g         2038 t
ORIGIN      
        1 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
       61 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      121 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      181 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatag
      241 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      301 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      361 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      421 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      481 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      541 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      601 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      661 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      721 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      781 tccggatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
      841 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
      901 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
      961 atgacaacct cggtcatcag gagggggatt tagcgattac gttacatgat gcgtataaag
     1021 cttccgatga gcgtttatat gtcaataagc agaacaaaaa cagccgttca tgataacctt
     1081 ctgtggttgt ttgcttgtaa tctcaggagc gtgaaatgac tgaaatggct aaaggaagcg
     1141 tgacgcatca gcagttaatc gctttattat cacaagaagg tgctaacttc cgcgtcgtta
     1201 tccatgaagc ggtaggtaaa tgtgaagcgg tgtcagaaat tcgtggcacc gctctaggcc
     1261 agggtgctaa ggcactggta tgtaaagtca aaggcaatgg cgtaaatcaa catgttctgg
     1321 cgatcctcgc agccgatcaa caggccgatc tgagccaact cgccagccat ataggcggat
     1381 tacgcgcttc gttggccagc ccggcggaag ttgatgaact cacaggctgt gtcttcggcg
     1441 cgatcccccc tttcagcttc catccaaaac tcaaactggt tgccgaccct ctactcttcg
     1501 aacgatttga tgaaatcgcc ttcaatgcag ggatgctgga taaatccgtt attttgaaaa
     1561 ccgcggatta tttgcgcatt gcgcaaccag aactggtcaa tttccgccgc actgcgtagc
     1621 tagcctatcc gttcaataag cagaacggac aaaaccagca caaccgcaat agcaaaaaac
     1681 gatgatgtga taatcagtgt ctcgacgaac atttgatcgt tcatagcatg cccccgggag
     1741 atagctgttt tcacgttatt gttagcgtgc acaaatggca gtttgatgac agttcgcctg
     1801 attttttatg caaaaaacgt aaagattttc tacttccttc ctgcagcaag cgtaaagtaa
     1861 gcaggcttat tattttttgg caaggaaacc acgatgtttg atgtcacttt gctgatcctg
     1921 ctcgggttag ctgcgctggg ctttatcagt cataacacca ctgtcgccgt ttcaattctg
     1981 gtgttgatca ttgtccgcgt cacaccgtta agcacctttt tcccgtggat tgaaaaacag
     2041 gggctaagta tcgggataat cattctgacg attggcgtta tggcccccat cgccagcggg
     2101 acgctaccgc cctcaacctt gattcactcg tttctgaact ggaaatcgct ggtggcaatt
     2161 gcagtaggcg ttattgtctc ctggctgggt gggcgcggcg tgacgttaat gggcagccag
     2221 ccgcaactgg ttgctgggct gctggtgggc actgttttag gcgtagcgct gtttcgcggc
     2281 gtaccggttg gcccgcttat tgccgcgggt ctggtttcgc tgattgtggg gaaacagtag
     2341 ttaatcccgc aatatatcgg cctggcgttt gccctaggcc tttcttaaac atagtaataa
     2401 aggccgttgt tgaatcatac cccagcgtat gagcaacttt ctgtacagta tcgcctttca
     2461 ctaatccctg aagcgccata atcagctgta actgctgacg ccactggcga aaactcaacc
     2521 ccgtctcttt cactatcagt cgggcaagat tgcgttcact catcgcaaaa aagccagccc
     2581 attgccccaa tgacccccac tcgacgggcc ctttcgccat catctccacc atcgtgcgga
     2641 ttttaggatg agaagaaacg ggcaggtgca gctgttgttg tggctgctga gggagttcat
     2701 caaacaggac ctgaattaat ctgcgggtca tcggctcagc acgctgagtc gtcgtcctgt
     2761 ttgccagggt taaaattaat tcccggcata agggcgatat tttcaatgtg caacatgttg
     2821 tcggcattgt cactgcgctg ggttcgataa acagaaaaca gagttcagcg ttagctgtca
     2881 cttgattgct atgcttcacc ccgcccggta tccaaaccgc atattgtggc ggcaccatcc
     2941 acaaagcatt ttccaccgta caggtaattg caccatgtaa tgctagtatc agctgtcctt
     3001 tgcggtgctg atgtacagga ctggttaact catccgttcc ggcgtgaata caaaatgcga
     3061 ccgcggcttc atggtgtcgg tcgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctagcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcccactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgctttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc gggcaagttc
     4201 tgcgcatcag gcggcggcac tctcggggat ggcacaatcc gtcgggtatt tgctggcagc
     4261 ctgtgggccg ccgctaatgg gtaaaataca cgatgctaac ggtaactggt ccgtaccact
     4321 gatgggtgtt gcaatacttt cactactgat ggcgattttc ggactttgcg ccgggagaga
     4381 caaagaaatt cgctaatatc cggtgctata gtgacgtgac aaatcttgcg tgaaagggag
     4441 aacaaacacg atgaatattc agtgcaaacg cgtttatgat ccggctgaac agagcgatgg
     4501 ttatcgcgta ctggtcgacc gcctctggcc gcgcggtatc aaaaaaaccg atttagccct
     4561 tgatgagtgg gataaagaaa tcacgccgtc aacggaactg cgcaaagcct ttcacggcga
     4621 agtcgtcgat tttgcaacct ttcgcgagca atatcgcgca gaactggcgc aacacgagca
     4681 agaaggaaag cggctggcgg acatcgccaa aaaacagccg ctgaccctgc tctacgcagc
     4741 aaaaaacacc acgcagaacc atgcgctggt gctggccgac tggctacgta gcttgtgatt
     4801 ttagtacggc atccggcggt tatttttcac cagccggatg gtcccgtcgc cacaatgccc
     4861 attcatcaat cgtttcaccg cccggtaatt tgcaattgtt gctgacccct tgcgctgtct
     4921 gcactggaat gagcgtcccg cccttctgct ggcaatagac cgacgccgga tttgccatac
     4981 caatctgcgg cggtttaggt gcttctggct gagaaggggt tgaacaacca gccaggacca
     5041 gcaagcaagg cagaacaaaa ctgataattt tcatttattg atctcacata tttatccaag
     5101 cttagagtat cgcggtatcg ttttgttttg cagcactatt tttattacat tcactcaaaa
     5161 catattacgt cttgtttcat ctttgttgat gatgttttat catgcctgca aagattaaat
     5221 aatcagcact tacccgccgt atcctggagt tgttccgtgt cagatcagat tatcgcccgc
     5281 gtctcgcaat cccttgccaa agaacagtca ctggaaagtc tggtccgaca gcttctggag
     5341 atgctggaaa tggtcactga tatggaatca acctacctga ccaaagtgga tgtcgaagcg
     5401 cgcctgcaac atattatgtt tgcccgtaac agccagaaaa tgcacatccc ggagaatttt
     5461 accgtctcgt gggattactc gttatgcaaa cgcgccattg atgaaaactg ctttttcagc
     5521 gatgaagtcc ctgaccgttg gggcgactgt attgcggcac gcaatcttgg catcaccaca
     5581 tttctgagca cgccaattca cttaccggat ggatcattct atggcacgct ttgcgccgcc
     5641 agcagtgaga agcgccagtg gagtgaacgc gcggaacagg ttttacagtt attcgccgga
     5701 ctgattgcac aatatattca aaaagaggca ctggttgaac agctgcacga agccaatgct
     5761 gcgctgattg cgcaatcgta taccgactcg ttaaccgggc taccgaatcg gcgggcgatt
     5821 tttgaaaatc tgacgacgct gttttccctc gcccggcatc ttaaccataa gataatgatc
     5881 gcgtttatcg atctggataa cttcaaatta atcaatgatc gttttggtca taatagtggc
     5941 gatctgtttc tcattcaggt tggcgagcgc cttaatacgc tccagcaaaa tggcgaagtt
     6001 attggtcgtc tcggtggtga tgagtttttg gttgtttcac taaacaacga gaatgcggat
     6061 atttcgtcgc tgcgagaacg tattcaacag caaatacgtg gagaatatca cttaggtgat
     6121 gttgatttgt attatcccgg tgccagtctt ggcatagtag aagtcgatcc tgaaacaacc
     6181 gatgcagaca gtgccctgca tgctgccgat attgcgatgt atcaggagaa aaaacacaaa
     6241 cagaaaacac cttttgtcgc gcatccagcg ctacattcct gaggcgtatt cacatccttt
     6301 tgattggtga taacatgcga atcggtatta tttttccggt tgtaatcttc attacagcgg
     6361 tcgtattttt agcatggttt tttattggcg gctatgctgc cccgggagca taaagatgaa
     6421 aaaaacaacg attattatga tgggtgtggc gattattgtc gtactcggca ctgagctggg
     6481 atggtggtaa cgtcacctct aaaaaatagc aaaggctgcc tgtgtgcagc ctttgtgcaa
     6541 tttaagcgtt aacttttaat cttcctgtag ataaatagca cgacaatcgc accaataacg
     6601 gctgaagcat ttcgtctgca gtctgaaccg caggcaacca tttttcagca agtctgcgct
     6661 gggaatcgct ggctttatcc catttgctcc acagtgcctc acgatcgtaa tcgtgcgctt
     6721 ccagcgtggg gcgggcgatt tcaaaatacc cctggcttgt ttctatttgc tcagatttta
     6781 ataacaactc aacacgtgct gccgctggta gaactgatat atgaaattcc aaagcctttg
     6841 aacccgtacg cttgcgacgt agttctggat gattggctgc aagcttttca agattgcggc
     6901 gaatactcac ctcatgttta ggaaagcatg acattcctac gcattcttgc gctgttaccc
     6961 aatgggttaa catgatgttt tctccatata ccgagaaggc caaatctggg ctggttccat
     7021 atccaaatat ctagcaatga ttgcttcgta tcgtggtata tggcagcgaa atacgttacg
     7081 catagtgtca ggctttaagc ctgcctctct ggataattga gatagtgtct tccccttcat
     7141 acgaacagca gcctttatct gctctgcgtg ccagtcttgg ttttttgctg acattctgcg
     7201 atccttactc tcttaacgtt ttcggtaact gataccggct accgctatcg ggaaaaacga
     7261 tatcgcagaa aaatgcgcat ttcaagagct atgcacattt ttattttaac caatgcaaat
     7321 caaagaattc tgtaaaattc tttatataac agagagattg agcatgacag gtgaaaaaga
     7381 aagcaaaaat gcagagcgca tttttatgcc ccggaaataa tgcgtttcaa ggaacgcctt
     7441 gaagaggcaa tgaatggaga atcaagccgg g
//
LOCUS       DADLTF010000171         7459 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.173, whole genome shotgun sequence.
ACCESSION   DADLTF010000171 DADLTF010000000
VERSION     DADLTF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.173"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..233)
                     /locus_tag="JN749_003610"
     CDS             complement(<1..233)
                     /locus_tag="JN749_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369876.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="JN749_003611"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="JN749_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HAY7369877.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="JN749_003612"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="JN749_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HAY7369878.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="JN749_003613"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="JN749_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HAY7369879.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="JN749_003614"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="JN749_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="JN749_003615"
     CDS             complement(3305..3424)
                     /locus_tag="JN749_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAY7369880.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /locus_tag="JN749_003616"
     CDS             complement(3385..3570)
                     /locus_tag="JN749_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369881.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /locus_tag="JN749_003617"
     CDS             3671..3844
                     /locus_tag="JN749_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369882.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3906..4190
                     /locus_tag="JN749_003618"
     CDS             3906..4190
                     /locus_tag="JN749_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HAY7369883.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="JN749_003619"
     CDS             4194..4529
                     /locus_tag="JN749_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HAY7369884.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="JN749_003620"
     CDS             complement(4586..6907)
                     /locus_tag="JN749_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HAY7369885.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPIPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="JN749_003621"
     CDS             complement(7024..7233)
                     /locus_tag="JN749_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369886.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2047 a         1753 c         1654 g         2005 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtgtggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg gggataggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgctt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac cttattttca tgacattgat cactttgggg tgactatttt
     7441 gtttaacaaa taactcttc
//
LOCUS       DADLTF010000172         7458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.174, whole genome shotgun sequence.
ACCESSION   DADLTF010000172 DADLTF010000000
VERSION     DADLTF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.174"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            214..290
                     /locus_tag="JN749_003622"
     tRNA            214..290
                     /locus_tag="JN749_003622"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:248..250,aa:Arg,seq:tct)
     gene            complement(336..1097)
                     /locus_tag="JN749_003623"
     CDS             complement(336..1097)
                     /locus_tag="JN749_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7369887.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINYYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1280..2170)
                     /locus_tag="JN749_003624"
     CDS             complement(1280..2170)
                     /locus_tag="JN749_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HAY7369888.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="JN749_003625"
     CDS             complement(2171..5149)
                     /gene="nfrA"
                     /locus_tag="JN749_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HAY7369889.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDP
                     ALIRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVG
                     RRWTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLS
                     VYSRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTML
                     RASASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANG
                     QTIEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFK
                     AINQRNGERNNAFLTIGVHW"
     gene            complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="JN749_003626"
     CDS             complement(5136..7373)
                     /gene="nrfB"
                     /locus_tag="JN749_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HAY7369890.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVISNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHGAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQAQAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
BASE COUNT         1737 a         1938 c         1951 g         1832 t
ORIGIN      
        1 ttccgttagg gcggtaaatt gtactttgat acatgaaaat acgggttttc ttgattcaga
       61 cgcgcagcgg tgtgcgtttg tttgccgcta tagcgaaata aatcagaaaa tcagacgcgg
      121 tcgttcactt gttcagcaac cagaccaaaa gccattgact cagcaagggt tgaccgtata
      181 attcacgcga ttacaccgca ttgcggtatc aacgcgccct tagctcagtt ggatagagca
      241 acgaccttct aagtcgtggg ccgcaggttc gaatcctgca gggcgcgcca tttcttcttc
      301 atttatgccc gtcttatccg tttccgcttt gcccttcacc acatcacttt ttgtcgctgt
      361 ttggcgagat aattcaacgg tgtcaggctg taaaacgcct taaaaacaga gataaagtac
      421 gacgtgctgc tatagccaca taattgcgcc acctgagtga tatttttgtt atccatcaat
      481 aacatctgta cggcgtaacg catacgacac tctgtgacaa tctggctata gctggtattt
      541 tcgtttttta atttcttttt gagcaggctg gggcttaaac atagcgaact ggcgacaatt
      601 cgcagattcc agtaatgctg aatatcgctt tgaataatgc ggcagacgct gtcgcggacg
      661 ctgctgcgta agatatacat cagtagggca ataaaccgcg attgctcaag aaagttagac
      721 aatacggtaa aaagtaatgc gcgcgtcaac tccgcttcgc agggtttgct gggttgctgc
      781 ttgctgtggt tggcggctag ccggaacact tccggtgtac ggctatgaca agcgataacc
      841 ggggttgcca gccgcgacca gggctttaca caggttaatt tcttatttaa aaacaacaga
      901 taatagttga tgatgttacg gttgatgtgg gtgattaata cgtgatttaa cgttgagagt
      961 tcaatgacgt tattgttgca cgcgaaggcg gccaggtagt ttttcggaag ggtaaacgta
     1021 gcatggttat tgacgcttac ctctacctct ttttcggtca ggatcaccac gcaaggttca
     1081 ctgctgctca attgcatttc gcactcctca gatatcagaa actccgctca aaggatctat
     1141 gcttcctgca tgagtgatcg gcccgttcgc cgataacgat cttctttctt tagcatgctt
     1201 tttagcaatt aatcttgatg gaattctgac gagagcgaaa gaggtaagcc aggtcgtacc
     1261 cgacttacct ggaggggatt taatactcga gaatgccgtg cgcgacgggc aaatagcgca
     1321 gagagaaata gagcatgtct ttaccgcaag aggaacgttt cgctaacagc gaggcaattt
     1381 ctgcgtccgg tttcggtttc gctgtaaagg ttttgccttt gccggcgaca aacagttcat
     1441 aaacaatgcc gcttgctggg gcggagcttt ggcaatcggc gctggcctgt agataacgtt
     1501 cacgctgttc agcggtaagt ttatccacgc cgctgccatc ctgtacccag acattaactc
     1561 cggttgcttt aacttgttcc agcagttggc gatagccatc gggcgacatg tttccggcga
     1621 aaaaactact gatataaacc ggttttgctg aaacatcgct aatcagccgc tgcgcgttgt
     1681 ttaaccatgt tagcaaaggt tgacgggcgg cttcgctgcg ccagttcagg tcgtcaattt
     1741 ccgcgctgat gtaccagcca tccggcgtta tgccaggcgc ggcgctccat aatctggctt
     1801 gctggagatc ggcagccagc aggcgattaa gatagctttc cagcgctgca gacgactgtt
     1861 tctggtgcat aaaaaattcc ggatcggcgt tcagcccgac aatcagcttc aggccagtct
     1921 gttgcgcagc tgcggcacgc ttaaacaata acgtgcgctg ttctggctgg gtaaatgcat
     1981 cgccgtaacg ggtccattgc aaaaccaggg tatcgaagcc ttgaaaacgt aactgactca
     2041 tcagcccctg ccactgggta tcggtaacct gactatctcg gttttgtggt tgccagataa
     2101 tacctttcat cgcaaaggaa aaagggctga ccaaaagcag tgtcagcaat acgaaaatga
     2161 acttacgcat ttaccagtgc actccaatgg tgagaaacgc gttgttgcgc tctccgttac
     2221 gttgattaat cgccttaaag gtatgttgat actcgacgcc gagactgact ttgtgcggcc
     2281 aggcgtcgta gtgcgtctcg ccggtccaaa tattccagcg gaccccgaca ccgccaagct
     2341 gcgcgccctg agtgccttta tcacgatagc cgttgtcctg aacgtgagcg taaggctcaa
     2401 tagtctgtcc gttagctacc ttctgatgcc agctgacgcg ataatctgcc gtccacgcct
     2461 gaatatcctg gcggatatat tgcgccgcat cgaggtacag gttttgggca aaccagcctg
     2521 aaccgttcgg gtgccattcg tcgctgtatt tgccgccatt aaagaatgag gcgctggcgc
     2581 gcagcatggt atcggatgcg ccattttgcc cgttcagcgg caactgctgt tcgacggcga
     2641 ggaaaaagat ctgatcgcgc agcggcttcc agcgcagacc ggtgccggac atcggatttt
     2701 tcaccggcat catcaccccg ttttctccgg tatcggcaaa gacgcggcta taaactgaga
     2761 gcaggtcgcc ttccagcagc atattgcgtc cgatgcggta ctcggcttcc agttgtccgt
     2821 agctacgata gcttttccca ggcgctgcac cgccgacatt attgttagcg gtactcattg
     2881 cgccggaacg caagccgatg gaagaatcga aactgaacgt ccagcggcga ccgacctcct
     2941 catgcaaacg gcggaaattg aagcgttgct gattttgctc tggggtcagt ggggttatca
     3001 gcgcctgatt atcaatgtca tcaatcacca gccgggcgta gtgctgcgtc gcaggcatgt
     3061 catccagacg ctggttcacg taggccagtt gtcggatcag tgccggatcg tccggaagcc
     3121 ctttatgcgc ctgttcgagc atttcccgcg actgtgcgat atcaccgcta tcccacaagg
     3181 cgtaaccaag cgctgcctgg gtgttgctat tattcggttc cagttccagt tccagcgcgg
     3241 cgcgcaaatc actcaccgcg gccggaacat tatgacgttg gcgataaatt gtcgcccgcg
     3301 caacgtaagc gttggcagaa ggggcaatat tgattgagcg cgtgagatcg ttcagtgcga
     3361 gttctggctg agctgggata taacgttgcg catgcagcca ccagtattga gcattgtttc
     3421 ccagctcacg ttgttctgcc tgttgaagcc agcgatcgcg agccgcgcca tttcccgctg
     3481 cctgagcggt gttggcggcg gcgagcagat cctcattgct catgtcgtga agactgattt
     3541 tctgccaggc cgccagtgcg gtggcgtagt cctcaacctg atacgcctga taggctaccg
     3601 cacgatgttg ccaggcgctc ggttgtcgtt gttcggcctg aagccatgca tacaacgcca
     3661 caccgggtag cgtgtcccga taacactttg ccagacggtt ccaggcggca gcatcgtagg
     3721 aaggcgacat atcgcccagc aagcgaacta ttgccgggca attatctgca atacccggca
     3781 actgactttg ccactgacgt tgctccgcca gcggtaaggg tttcgataaa atcgccacct
     3841 tcgccggcgt tgctaggtaa ggatgacttt ccagcagaga cgccagtcgc gccattaaag
     3901 tctggctgac acgcgcatcg ccctggaaag gatagcgttg cagcaataaa tcggcagctt
     3961 cgcgtgactg ctcgttctgc atcagttgcc aggtcagttg atccaggcgg ggaagatttg
     4021 tcggttcttg ctgatacagc aatcgtgcca gacgcagaga ttcagccttg ttacgggtcg
     4081 ccacgctgac agcataacgc tcctcaagca tttcattggc ggggagggtg gcgagcagtt
     4141 tttgcgctgc gtcgtactga ccctctttta acagcaccgg tagcgtcgcg ccaacaacat
     4201 actggcggtt atcggcaaac tgcaccgtat aattcgccaa tgcctgaacg gggttggcgc
     4261 tgtatttaga taacagatag agccaacttt tctcttgtgc gcccgtggta aacagcggct
     4321 tattttcaat gagataatgc tggaggcgtg ctttttcgcc acgataagcc agcgcggtcg
     4381 cgtaagtaat atatgactga ggatcggtga agatcccctg tgattgcagt gccaggatcc
     4441 gatcgtccag ctgcccggca agaagcacgt caaaccactg acggcgttct gccgcgctta
     4501 atgtgttctg ctggcgtgct tcattgtata gcgtatctgc ctgggaccat tgtttcaggt
     4561 agattgcccg ttgcagcaga tcggttcgca gcgtttttcc ttccggcgat gcagcaaacg
     4621 tcgcatcgtt cagttgcgct ctggcgacag gtaattgtgc cagccgcagg gcattctgcc
     4681 cgacttcact gcgacaacgc agggtcggcg cagcgtcgcc cgctttttgc tgggcaagca
     4741 gttcttcaac agtcgtaacg cttttcactt caactggaat agccgccaga ctgcgctcaa
     4801 gtcgggcatc tcctgggtga cgtttcagtt gatcctcaag caacagccgc gcccggtcat
     4861 catgaccaaa atgacgatag gcttccgcaa ggtataaagt cagcggaata ttatccggca
     4921 cctgttggtg tatatattca aattcgcgga tggcggttgc ttcgtcgtta tttttctgtg
     4981 ccttcagcgc cttatcgaga cggggataaa taacaaaatg gcgataatcg ctcagcccca
     5041 gctcttctgc gctggtgccg atattgtctg cgagtgcgct ggtactcaat aaagacgtca
     5101 gcagtaaacc agaccatccg atgacgcgat taaggttatt ctccttcatt ttcggactcc
     5161 agttgcgcaa cctgttctgt gtttaaacct gctttgagta atagtgattg catcgaaact
     5221 tgtaattcgc gttgaattgt caggacgcga tccaacgttt cctggctgat aacgccttcg
     5281 gtgaccaaaa acttgccgag cggcagagaa ctgcgttcat ggcgcaataa caacacgtta
     5341 attgctgaac gattaatatg accgagcgtg gtcagtattt cggcgaacag gaactgatgc
     5401 ggcacatatt gccgccagat ttcaccggtc tgctgttccg tgagccactg atgctgaacc
     5461 gcattgtaca acattgcccg cggatcgtga ccgcgtcggc gtgcatacca gtggcgtaac
     5521 cctgtgacaa tttgtccccg cagaacaatg acgtaacgca ctttgcgtcc gactttacgc
     5581 gtcagggccg ccagcgaaac cgggtcaata ccatcttcac tgccgacaat taactcatca
     5641 ttttccagac gcagcggcag tactgcataa tgcagcgcta cggaggctgg catttcggca
     5701 atcagcgagg acgggatctg ccaggcatcg atggattccc acgccacgcc gttttgctct
     5761 gcctgcgcct gtgccagctg ctcggcgcta atcagcccct gcatcagcat tgaaccgccc
     5821 aggcgtagac cttcgacgcg attacgcagt gctgtatcga gttgttcttc agtgatgacc
     5881 tgattttcca gcagaatttg acctaacggg cgcaacgagc gggtatcgcc agtgacgctg
     5941 gggaagtcat gcgttgtttt atcccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgcca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc tgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agccgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt atgaattgtg
     7441 acagcttttt accattaa
//
LOCUS       DADLTF010000173         7320 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.175, whole genome shotgun sequence.
ACCESSION   DADLTF010000173 DADLTF010000000
VERSION     DADLTF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.175"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..195
                     /locus_tag="JN749_003627"
     CDS             <1..195
                     /locus_tag="JN749_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7369891.1"
                     /translation="APMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRH
                     SRLGNISPAAFREKYHQMAA"
     gene            complement(239..538)
                     /locus_tag="JN749_003628"
                     /pseudo
     CDS             complement(239..538)
                     /locus_tag="JN749_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(722..934)
                     /locus_tag="JN749_003629"
     CDS             complement(722..934)
                     /locus_tag="JN749_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HAY7369892.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(1065..1625)
                     /locus_tag="JN749_003630"
     CDS             complement(1065..1625)
                     /locus_tag="JN749_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HAY7369893.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPWLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            complement(1680..2426)
                     /gene="traX"
                     /locus_tag="JN749_003631"
     CDS             complement(1680..2426)
                     /gene="traX"
                     /locus_tag="JN749_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HAY7369894.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            complement(2446..7311)
                     /gene="traI"
                     /locus_tag="JN749_003632"
     CDS             complement(2446..7311)
                     /gene="traI"
                     /locus_tag="JN749_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HAY7369895.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQYNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFMYTDLLARTVGILPPEN
                     GVIERARAGIDEAISSEQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHP
                     EKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIA
                     ADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGA
                     ARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARL
                     AGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDS
                     RSRYLRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQVETQVVRISSLD
                     SSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPAT
                     LPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLY
                     SSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALP
                     VLESKNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLL
                     VSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAATRMILETSDRFTV
                     VQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFL
                     HDTQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIA
                     PGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPR
                     LEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEA
                     REQTLIVTHLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWE
                     NNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRV
                     GTGDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLA
                     YAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQAYTDNRQGWTD
                     AINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPT
                     RFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQF
                     VALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIP
                     DNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQA
                     VREIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVR
                     DLQKEKTPGGD"
BASE COUNT         1423 a         2371 c         1833 g         1693 t
ORIGIN      
        1 gctccgatgg aaagcttctg gggaacgctg aaaaatgaga gcctgagcca ctatcgtttt
       61 aataaccggg atgaagccat ctcagtaata cgggaataca ttgagatttt ctacaatcgt
      121 cagcgtcgtc actctcgtct ggggaatatc tccccggcag ccttcaggga aaaatatcat
      181 cagatggctg cttaaaaaaa gaacaaatgg tagtgtccgc tattgccagt acacctcaat
      241 atgtgacggt aaccgttttt cctgcgacca gtgatttcat catgtctgtt gaccaccggc
      301 cataatcctg tttcttttcc ggtgcatcaa tatttaccag cctgatccgc accgccttca
      361 gtgagtccat gacctcgatg gtatcaccgt ccagtacacg aacaacccgt ccatgaatat
      421 ctgcagacaa taccggccat gaaaagataa ataataccag tggaatatat ttcctcatgt
      481 tattctccat atcagataaa tgccacctgt atttcagccc ccctgaatag tgcagcacac
      541 attgaataac accttatttt gtgaaattaa tcacaaaata taaaaaatcg acttaatgcg
      601 gaaaagaccc tgtgctactc agaactgtcc ggtcatactg cccgaccgga tattaagaag
      661 caaatctcat aaactccttt cagccttctg actcctagag taatgaaggc tgcttttttc
      721 gttacaccct tccccggcag cgccggtggc agtgttcagc ccttttccgg gcaaactgct
      781 gccagtctgt cctaggcgca acacaggcag cccgtcgcca tgcttctgcc gcttccatcc
      841 agcgtccact gacttccaga ataacggccc gcccgccggc acgctggtaa cgccagacct
      901 ggccgttccg tgaactgttt ctgaatccat tcattttctt gtcccccttt agttgtcagt
      961 attattgtga taaataattc agtggactgg cttcctttcg gaagcggaag ttatttcctt
     1021 ttaagcctta tttccctgcc tcctctctct gggggaggct ttttttattt ctcatcaagc
     1081 acggcctgaa gttctgcctt tatccggttc tggcgacgaa ttttcgccag acgcgctccc
     1141 gcatacgctt cctcctcctg agaaatatgc tccgtcacat acccttccgt gtcataccgg
     1201 caggcaccgg ctttcatggc acacagatag ctttctgaac gggtgatggc cttcagcgcc
     1261 ctgcgcagtt ttttatgcga gagcgggata ttccgctgcg ccacgtcttc cagtaacacg
     1321 tcccggatac cgcaggccag aagccagggc gtgtcaccgt caaataatcc cggccaccag
     1381 ggcttcaggg tattcacagc ctcatccagc gtgggcaggt tcagataaat ggacagcgcc
     1441 tgtctggcct gcgctttttt tgccgccagc tctgcttcac gggccgcttt ctcggccagc
     1501 ttctgctttt tcaccttcca ctttggtggc gtggtgacat tgatgatggt tttccggctg
     1561 cggacaggcg ctgttccctc tgtcttccgc ttcagtgtca gtaccggtcg cttctgctct
     1621 atcatgctgt gttctgcctc tgacgaatca tcctcttgcg gccaaagata ccacacccgt
     1681 cacagcgcca gaacgcccag cacagccaga tgacaggcat agaacgtcgg gaaaaagtct
     1741 ccgggccaga aacggggtaa tgattttccg gcacacaaca ccagaccaac ggtcagcacc
     1801 gtcatcacaa gaccggccac cgccgctgca tcactggtgg caaggttaag cgccggaata
     1861 acagccagca ggcaggcgac cagcgccaga cgttccgctc tgtcttccgc ccggtacagc
     1921 cggttgctga ctgccagcat cagcaacccg gcaataccgt aactggtgcc ggacaaaggc
     1981 ccccacaggg ccatcaggag tatggccgct gctgtacgcc acccactgcg cgtttcacac
     2041 cacgtcagca cctgtgccgc cactgcaaag gcaaacagga tattcccctc ataccaagga
     2101 aagcctgcca gataatatgc gaactgggca ataattcccc atccccacag ccggttaatg
     2161 gccggttgcc ggatatgcgc atgacgggac agattcagcc cccacaccag ggcaaaaagc
     2221 ggaaaggctc ctcgcccggc cagaaacatc cattcctgct tcagctggaa tatcagattg
     2281 atgtggtcca gtaccatcag caccagtgcc accgttttga tgatatcccg ctgtccgggt
     2341 gaccagacca gaaatgactg caaccaggta tcggtccgtg cagccagtga atcgttacgt
     2401 gtcgtgttcg tgttatccgt tgtcatcaga gtttgtcttc ctgtatcagt ctccgcccgg
     2461 ggttttctct ttctgcaggt cacgaaccat atcccgctcc atctgctgca gacgctcctg
     2521 cagcaaattt tcccgggcaa cttcccggac cgcttcccgc acccgttgtg gttcacgcag
     2581 tacactctcc ggcagcgcgg cttcccgttc agttatggca gcccggtcac gctcctgacc
     2641 ggcaatttcc ctgacagcct gctctgtttt gccgtccggc aggtcaggtt tgttctctgc
     2701 cattttacgg acaatttcat ctgcgcgacg ttccgtttca cggcggatgg cctcttcagc
     2761 ctgccgctgt gccagtacct ctgccgtgac cggttcgcca tttccggctc ccggctggac
     2821 gctgttgtcc gggatatccc cccacacgcg gccaccggtg atggcaccgg gattccacgg
     2881 acggccttca ccggcgattc ttaccaccac accactgtca ggattatcac gggcaatccg
     2941 gacgccatcc tgcatattat cagccagcag gctttctccg ttacggctgc cctgcagggc
     3001 cacgaactgc gcatccccgc tgccttttac ccgaccttca ccgctgaatc cccgcagccc
     3061 gtttccgtca tccgtggtca gcgggttcag ccagataccg gcggacttgc cgttacggtc
     3121 aaacgccggc agtgccacat acggttgcgg atatttacgt cccggagcaa taaaccgtgt
     3181 aggactgtct cccccggcca gccccgcctg acggagaacg gcacggcctg ccgccacgtc
     3241 ccgcagctcc cgcgccgtac tgaacagccg ctctgcattc atgacctccc ggtccggttt
     3301 cggctcaaac acatcgtggg cagttccttt ctgtacggca ttgttgatgg catccgtcca
     3361 gccctgacgg ttatcggtgt acgcctgcac atgctgcttc atacgcgaca gggccacgta
     3421 ggctgactca aagccggcca tcagtttccg gttaccttcc gtgccttcaa gcgcgatggc
     3481 aaaggtttca ctcgccccct gtgcaccgtg ggcggtgatg gcataggcca ggtcaatatg
     3541 ttgctctgcc cgctcctgac cggggcgaat cacacgggtc tgctgaccgt cagaaagtgt
     3601 gacactgtca ccggaaaccg ctgtcaccgt ccagacgctg ttggccacat aaccgcgctc
     3661 ccggtcactc ttcgtgaagc gtatcctgtc accggtcccc acccggatgg tgtccggggt
     3721 gtacagcgtg acgccttcag ccaccgcctc ccggggcgaa atcagccgcg tgttaccttc
     3781 cgcatcctgc agggttatca gcccgtcatc cttgctgata ccggcaatcc ggtgatacac
     3841 attatccacc agggcaaggg catccgggtt attctcccag gtggagagac gacgcagctc
     3901 cccgtcgcgt atattcgctg tgttcaggac aggcaccatg acctgcacct tccccagttc
     3961 tccggccttt tcccgtgcat catgaatcat gctgttcagt acgcgccggt cctcattcag
     4021 gtgcgtgaca atcagcgtct gctcccgtgc ttccggtgtc ctgccggtat agtcgcggac
     4081 aatggcttca tacagtgtca tggggacatc cggaaaagcc tcgcctttca gcatcgcctt
     4141 ctgctgcgct tctgccagtt tcgcttcctg gctgtgactg aactccgtca cggagtgctc
     4201 cggtgcccat gcgccctcca gacgtggcac ctgagacggt ttcacacgct caagcccgga
     4261 cagcgccctt tccacatccc ggttaatcag gctgtatacc gcctcccgca gttccggcgt
     4321 ctgacgcaca atctccttca tgatgaccac atcggcagca ctgcgcgtct gctggagacg
     4381 gaaaggctga ccgggcgcga tggcctgcag ctggtccgtg tcaccgctgg ccacagcccg
     4441 accgccaccg gccgcaatca gggcgtatgc ccgtgccatg tcggtattgc ccaccataga
     4501 gctctcatcg agaaggaaca gcgtgttgct gaaatccggc gtttctccgc tgcgctgctg
     4561 cgtgtcatgc agaaaggacg ccagtgtctg tgcatccacg ccggcgctgc gcatctcccc
     4621 gaccgcacgg tgtgtgggcc ccagccccac gacacgggga cgttcactct ccggcagcat
     4681 gttcacggct gacatcaccg cccggaactg tgtggtctta cccacaccgg catagccctg
     4741 caccaccgtg aaacggtcag acgtttccag tatcatacgg gtggcggcgc gctgtcctga
     4801 tgtcagtttc tccatgagtt cgccaggtac tctctccatc agcggcgtga ccgcctcctt
     4861 accttcgaga atatggcgaa gaatgctctt ttctgcctca tacgacgcac gggaaaccag
     4921 caggcctgtg ccatagcctt ttgccacatc cacatacagt aaatcacccc gttttatctg
     4981 cgcattgatt tcccctccca ggtcagcaaa accggttcct tctgcagcaa acgacttcgc
     5041 ctctgtcagc aggtccacca tgctgaaggc caggttttta ctttccagca caggaagggc
     5101 cagatgaatg gcctgctgtg ccggcgtgtg aagcccggct ttctgcagac tgatagcggt
     5161 ttccagcaat gtctcaccgg cacgcgcctt tatctgctca gaaaccaccg taaaggaggg
     5221 atggcgggca agtttttccg cagtacgggt ttcatccagt gaggaataca gccggacatc
     5281 acgaccactg cgggccagac cattcagggt ggcattgtcc attgccatct gtgtgacgga
     5341 ggcaaaaacc gtcgcactgt cgctgacgga atgcccgggc gtttccaccc agccgttctc
     5401 cagcttcagt gccgtgaacg gtgaatcgct cacaggcagg gtggccggtt cagcccgtcc
     5461 cggcacaaca accgtcatcg catcttcact gacggatgcc acctgcaggc ggtcaccgcc
     5521 ggagacgcgg agtccgggaa ttttccctgt caccctcaga cgctcaccgt ctgccaccgg
     5581 cattttttcc ggccggaaca gcgaccagct gctgtccagg gaagaaatac gcaccacttg
     5641 cgtttcgacc tgcgcattcc gcagggtcag gctgtgactc tgcgccgtca cacgttctgt
     5701 cacatagcgg tcatgactgc gtgtctccgg gttccactgc tccatcacca ttcccgggcg
     5761 gtacatatcc cgcagataac ggctccggct gtccagccag accggtgaca gggcggtcat
     5821 ggttacctcc gggtgtccga gcacgccctg tgttttcagc tcactgcgaa tggcctgtgt
     5881 cagtatggcc tgttcccgta ctccgctgac ctgtgccacg ctctcttctc cggctttcac
     5941 gctggccgca aaatctccgg ccagccgggc atagcggaca ttacggtccg gttcactgat
     6001 gatggtggcc ggtcgctgtt ctcccccctg ccagcgatat gtgttcaccc cggcatcctt
     6061 catggccatc agtgcactgc ctgtaccggt tcgctgcccg ctgtcggtta tcaggacctg
     6121 tacgttatga cgtgcggcac catccagcag ggttaacgtc tctttcaggg agagtttttc
     6181 gccctggtcg acgataacgg tactgcccgg cgtgaaggcc atgccttcca gcaactgacg
     6241 acgtccggtt atcagttcac cggacagccg ttcatcctgc ttcaggttca tctgcgagcg
     6301 acggtcagca gcgataatct gcacctcccg cccctgctcc cgggccatca tgaccagttc
     6361 agccacccgc tcacgctgcc cggctgcacc gccctgcccg gacacaatgg ccagcgacgg
     6421 acggtcctgt gccagaacac tgacggcatc gctgtaaccg gccgtccggg ggacactttt
     6481 ctccggatgt acggtcaccc ggttctgttt catgatgtca cgactgagtg cccggactga
     6541 cagctcatcg agcatatgaa tcccggacgt gaacagcccc ttctcacgat cgagggggat
     6601 aagctgctca ctgctgatgg cctcatcgat accggcgcgt gcccgttcaa tcacaccatt
     6661 ttccggcggc agtatgccga ccgtcctggc cagcaggtcc gtatacataa actgcacttt
     6721 gcgttcactt aatccggcaa tggcctgtgt caccgcctgc tgcacatccg gcccgtcctg
     6781 tgaagcgggt ccgggtgtct gcgtgcgggt atatgcccgt tgttccgctg cgtcacgata
     6841 tgcccggatg tcgaacccgg tttccttcag cgtctgcatc cactcggcca ttctgacttc
     6901 aggatcgacg tgctgtttgg atttacgcgt atccagcgcc gccacatccc gggatttcag
     6961 cgaggcgtct tccccgacgg cctcccggat agtctgtgag cgtccggaaa aggcctccac
     7021 cggtacaccc ggcatttccc acataccgtg cttaccgacc acttcagttt catagcccag
     7081 cgcctcaacc tgctctttca gtttttcccg gtagagcctg ccaaaggcaa tctgattagc
     7141 gtacacattc tcaatgaacc ccgttttccc cactttgtca ctgctcagtg tcttccattc
     7201 gccgttatac tgcgtaacat tagtcaccac cgcatgcgtg tgtaactgtg gttcctgatc
     7261 gcgactggtg tcgtggttaa acagtgccat caccagatta ccggtcagca ccgtttctga
//
LOCUS       DADLTF010000174         7288 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.176, whole genome shotgun sequence.
ACCESSION   DADLTF010000174 DADLTF010000000
VERSION     DADLTF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.176"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            91..432
                     /gene="yneK"
                     /locus_tag="JN749_003633"
                     /pseudo
     CDS             91..432
                     /gene="yneK"
                     /locus_tag="JN749_003633"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            462..1202
                     /gene="yneK"
                     /locus_tag="JN749_003634"
     CDS             462..1202
                     /gene="yneK"
                     /locus_tag="JN749_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011379213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HAY7369896.1"
                     /translation="MFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQFPMINLGAPI
                     KRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKD
                     PNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQESPHFQDLFL
                     FFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHE
                     DLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            1352..2542
                     /gene="ydeA"
                     /locus_tag="JN749_003635"
     CDS             1352..2542
                     /gene="ydeA"
                     /locus_tag="JN749_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HAY7369897.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JN749_003636"
     CDS             complement(2567..3232)
                     /gene="marC"
                     /locus_tag="JN749_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HAY7369898.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3444..3878
                     /gene="marR"
                     /locus_tag="JN749_003637"
     CDS             3444..3878
                     /gene="marR"
                     /locus_tag="JN749_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HAY7369899.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3898..4303
                     /gene="marA"
                     /locus_tag="JN749_003638"
                     /pseudo
     CDS             3898..4303
                     /gene="marA"
                     /locus_tag="JN749_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
     gene            4335..4553
                     /gene="marB"
                     /locus_tag="JN749_003639"
     CDS             4335..4553
                     /gene="marB"
                     /locus_tag="JN749_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HAY7369900.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="JN749_003640"
     CDS             complement(4584..5483)
                     /gene="eamA"
                     /locus_tag="JN749_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HAY7369901.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5747..6865
                     /gene="ydeE"
                     /locus_tag="JN749_003641"
     CDS             5747..6865
                     /gene="ydeE"
                     /locus_tag="JN749_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HAY7369902.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            6992..7087
                     /gene="mgtS"
                     /locus_tag="JN749_003642"
     CDS             6992..7087
                     /gene="mgtS"
                     /locus_tag="JN749_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HAY7369903.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         1902 a         1774 c         1658 g         1954 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
      961 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     1021 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactatttg
     1081 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     1141 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     1201 aaaccgtgat gttaccgact ctctgacgcg gaacagaatc agcgtcagag aaacggaaaa
     1261 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     1321 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     1381 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     1441 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     1501 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     1561 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     1621 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     1681 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     1741 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     1801 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     1861 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     1921 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     1981 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     2041 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     2101 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     2161 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     2221 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     2281 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     2341 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     2401 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     2461 ggtgcctgct tttgccgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     2521 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     2581 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     2641 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     2701 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     2761 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     2821 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     2881 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     2941 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     3001 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     3061 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     3121 cactgttcat gttgcctgcc aggccaagaa atagcgcaac agttgttaat gggttagcta
     3181 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     3241 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     3301 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     3361 gcatcggtca attcattcat ttgacttata cttgcctagg caatattatc ccctgcaact
     3421 aattacttgc cagggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     3481 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     3541 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     3601 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     3661 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     3721 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     3781 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     3841 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     3901 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     3961 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatgagcg
     4021 ttcgggttac tccaaatggc acctgcaacg gatgtttaaa aaagaaaccg gtcattcatt
     4081 aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga aggaaagtaa
     4141 cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa ctctgacccg
     4201 aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca atatgcaggg
     4261 cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa cgtgacaacg
     4321 tcactgaggc aatcatgaaa ccactttcat ccgcaatagc agctgcgctt attctctttt
     4381 ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt gccaatgtcg
     4441 tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg ggtactggca
     4501 gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg tagtttgttc
     4561 tggccccgac atctcggggc ttattaactt cccaccttta ccgctttacg ccaccgcaag
     4621 ccaaatacat tgatatacag cccggtcata atgagcaccg cacctaaaaa ttgcagaccc
     4681 gttaagcgtt catccaacaa tagtgccgca cttgccagtc cgactacggg caccagtaac
     4741 gataacggtg caacccgcca ggtttcatag cgtcccagta acgtccccca gatcccataa
     4801 ccaacaattg tcgccacaaa cgccagatac atcagcgaca agatggtggt catatcaata
     4861 gtaaccagac tgtgaatcat ggatgcggaa ccatcgagaa tcagcgaggc aacaaagaag
     4921 ggaatgattg ggattaaagc gctccagatt accagcgaca tcaccgccgg acgcgttgag
     4981 tgcgacatga tctttttatt gaagatgttg ccacacgccc aactaaatgc tgcagccagg
     5041 gtcaacataa agccgagcat cgccacatgc tgaccgttca gactatcttc gattaacacc
     5101 agtacgccaa aaatcgctaa ggcgatcccc gccaattgtt tgccatgcag tcgctccccg
     5161 aaagtaaacg cgccaagcac gatagtaaaa aacgcctgtg cctgtaacac cagcgaagcc
     5221 agtccagcag gcataccaaa gttaatggcg cagaacagaa aggcaaactg cgcaaaactg
     5281 atggttaatc cataccccaa gagcaaattc agtggtactt tcggtcgtgc aacaaaaaag
     5341 atggccggaa aagcgaccag cataaagcgc aaaccggcca gcatcagcgg tggcatgtta
     5401 tgaagcccca ctttgatgac cacaaaattt agcccccata cgaccactac cagtagcgcc
     5461 aacaccccat cttttcgcga cattctaccg cctctgaatt tcatcttttg taagcaatca
     5521 acttagctga atttactttt ctttaacagt tgattcgtta gtcgccggtt acgacggcat
     5581 taatgcgcaa ataagtcgct atacttcgga tttttgccat gcgtccgatg gttaataaca
     5641 acgacgaggc aagaagggcg ctggtagagc gtcgtaggga taagttcatt tgtctgtccg
     5701 gcagtgcgtt tcgttatgtt ttattttaga gatgtaaaga aatagcatgg ctacgctgcc
     5761 atttatgacc atttacttga gtcgccagta cagcctgagt gtcgatctaa tcggttatgc
     5821 gatgacaatt gcgctcacta ttggcgtcgt ttttagcctc ggttttggta tcctggcgga
     5881 taagttcgac aagaaacgct atatgttact ggcaattacc gccttcgcca gcggttttat
     5941 tgccattcct ttagtgaata acgtgacgct ggttgtgctc ttttttgccc tcattaactg
     6001 cgcctattct gtttttgcta ccgtgctgaa agcctggttt gccgacaatc tttcgtccac
     6061 cagcaaaacg aaaatcttct caatcaacta caccatgcta aacattggct ggaccatcgg
     6121 tccgccgctc ggcacgctgt tggtgatgca gagcatcaat ctgccctttt ggctggcagc
     6181 tatctgttcc gcgtttccca tgcttttcat tcaaatatgg gtaaagcgca gcgagaaaat
     6241 catcgccacg gaaacaggca gtgtctggtc gccgaaagtt ttattacaag ataaagcact
     6301 gttgtggttt acctgctctg gttttctggc ttcttttgta agcggcgcat ttgcttcatg
     6361 catttcacaa tatgtgatgg tgattgctga tggggatttt gccgaaaagg tggtcgcggt
     6421 tgttcttccg gtgaatgctg ccatggtggt tacgttgcaa tattccgtgg gccgccgact
     6481 taacccagct aacatccgcg cgctgatgac agcaggcacc ctctgtttcg tcatcggact
     6541 ggtcggtttt attttttccg gcaacaacct gctattgtgg ggtatgtcag ctgcggtatt
     6601 tactgtcggt gaaatcattt atgcgccggg cgagtatatg ttgattgacc atattgcgcc
     6661 gccaggaatg aaagccagct atttttccgc ccagtcttta ggctggcttg gtgccgcgat
     6721 taacccatta gtgagtggcg tagtgctaac cagcctgccg ccttcctcgc tgtttgtcat
     6781 cttagcgttg gtgatcattg ctgcgtgggt gctgatgtta aaagggattc gtgcaagacc
     6841 gtgggggcag cccgcgcttt gttgatttaa gtcgaacaca ataaaaattt aattcagcct
     6901 tcgtttaggt tatctctgct aatatctttc tcattgagat gaaaattaag gtaagcgagg
     6961 aaacacacca caccataaac ggaggcaaat aatgctgggt aatatgaatg tttttatggc
     7021 cgtactggga ataattttat tttctggttt tctggccgcg tatttcagcc acaaatggga
     7081 tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt tgtgtacaag
     7141 gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg aatgcttgca
     7201 tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg ataacggatc
     7261 gttgacacag tagcatcagt tttctcaa
//
LOCUS       DADLTF010000175         7255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.177, whole genome shotgun sequence.
ACCESSION   DADLTF010000175 DADLTF010000000
VERSION     DADLTF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.177"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..149
                     /locus_tag="JN749_003643"
     CDS             <1..149
                     /locus_tag="JN749_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7369904.1"
                     /translation="PDNEGHVSVRYAAANNLSVIGATKEDKQRISEILQETWESADDW
                     FVSE"
     gene            439..693
                     /gene="bssS"
                     /locus_tag="JN749_003644"
     CDS             439..693
                     /gene="bssS"
                     /locus_tag="JN749_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HAY7369905.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            808..1926
                     /gene="solA"
                     /locus_tag="JN749_003645"
     CDS             808..1926
                     /gene="solA"
                     /locus_tag="JN749_003645"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HAY7369906.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            1974..2087
                     /locus_tag="JN749_003646"
     CDS             1974..2087
                     /locus_tag="JN749_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HAY7369907.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2348..2914
                     /locus_tag="JN749_003647"
     CDS             2348..2914
                     /locus_tag="JN749_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HAY7369908.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2918..3493
                     /locus_tag="JN749_003648"
     CDS             2918..3493
                     /locus_tag="JN749_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HAY7369909.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3535..4587)
                     /locus_tag="JN749_003649"
     CDS             complement(3535..4587)
                     /locus_tag="JN749_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAY7369910.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4812..5732
                     /locus_tag="JN749_003650"
     CDS             4812..5732
                     /locus_tag="JN749_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HAY7369911.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5904..6101
                     /locus_tag="JN749_003651"
     CDS             5904..6101
                     /locus_tag="JN749_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HAY7369912.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            6125..>7255
                     /locus_tag="JN749_003652"
     CDS             6125..>7255
                     /locus_tag="JN749_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001250418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7369913.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT         1892 a         1708 c         1714 g         1941 t
ORIGIN      
        1 ttcctgataa tgaaggccac gtatcggtac gttatgccgc agcgaataat ttatcggtta
       61 ttggcgcaac aaaagaagat aaacagcgca ttagcgaaat tctccaggaa acgtgggaaa
      121 gcgccgatga ctggtttgtc agcgaataat atgcagtgat ttttgttttt gccgggtcgc
      181 cccggctttt ttattttact tacgaatttt gagtacaaaa tttctacact tcctcaaaat
      241 aattgcacaa tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct
      301 taaaagcaga aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg
      361 gtatactgaa gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac
      421 acgaattagg gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta
      481 gggtgggaca tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag
      541 accccaaata agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact
      601 gatgttgcca gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt
      661 gatttcccgg taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc
      721 acctcaccgg tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc
      781 ttaaaccatg agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt
      841 tccgtaggcg ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc
      901 gacgcccata tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc
      961 catgcttatg gtgaaggcga aaagtatgtc ccgttagtcc tccgcgcgca aacgctgtgg
     1021 gatgaacttt cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac
     1081 ctcggcccgg ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa
     1141 ctcaacgttg aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc
     1201 ccggacaact acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg
     1261 attaaaacct ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg
     1321 gtcaccgcaa ttcgtcatga cgatgatggc gtaactattg aaacggttga cggtgagtat
     1381 caggcgaaaa aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg
     1441 cctgtccagc ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg
     1501 aagaataaat tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt
     1561 ccggcagaaa acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca
     1621 gcggatgaac gtgttccgtt tgcggaagtg gccagtgatg ggtcagaagc cttcccgttc
     1681 ttgcgcaatg tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat
     1741 aattcgcctg acgaagattt tattatcgat accctacccg gccacgataa tacactgctc
     1801 attaccggcc tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc
     1861 gattttgcgc aagaccaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc
     1921 caataatcat cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc
     1981 ggctgttgca ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct
     2041 ggggattgtt ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga
     2101 cataaactga caaatattta atctcccttt catttagcgt gaaaaattga atgagattat
     2161 tcctgcttct acagcactca ccagacatct ctgatgctga acccactaaa cttatgaaga
     2221 ttctttcatt attaattcaa aattattgaa ataaaaataa atatcattca atttgttatt
     2281 tttgtgttgc aattcattct ccgacacgcc attttctttc tctcgcaaat tcgtattgga
     2341 tgcacttatg tcattcacaa atacccctga acgctatggt gttatatctg cggccttcca
     2401 ttggctaagc gcaattattg tctacggcat gtttgcttta ggtctgtgga tggtcacgct
     2461 cagttattac gatggctggt atcacaaagc acccgaactg cataaaagca tcggtatttt
     2521 gttaatgatg gggctggtta ttcgcgttct gtggcgtgtc atatctccac cgccgggtcc
     2581 gctgccgagt tattcgccca tgactcgtct tgccgcaaaa gctggacatc tcgcattgta
     2641 cctattgctg ttcgccatcg gcatcagcgg ctatctgatc tcaactgccg atggtaaacc
     2701 gatcagcgtt tttggctggt ttgacgtccc cgcgaccctt gccgacgctg gcgcacaggc
     2761 agactttgcc ggtgccctgc atttttggct cgcgtggagc gtcgtagtac tgtctgttat
     2821 gcacggattt atggccctga agcatcattt catcgataaa gacgacactc tgaagcgcat
     2881 gctgggaaag tcgtcatctg actatggagt ataaaaaatg aaaaaaagcc tgcttggttt
     2941 aaccttcgcg tccctgatgt tctctgccgg ttcagcggtt gccgccgatt acaaaattga
     3001 caaagaaggt cagcacgcct ttgttaactt ccgcatacag catcttggct atagctggtt
     3061 atatggcacc tttaaagatt tcgacggtac ttttaccttt gatgaaaaaa atccggctgc
     3121 cgataaagtg aatgtgacaa ttaacaccac cagcgtcgat actaaccacg ccgaacgcga
     3181 taaacatctt cgcagtgcgg attttctcaa taccacaaaa tatccacagg caacattcac
     3241 ctccaccagc gtgaagaaag acggtgacga actggatatt accggcgatc tgacgctgaa
     3301 tggcgtaacc aaacccgtca cgctggaagc gaaattaatt ggtcagggcg acgacccatg
     3361 gggcggtaaa cgtgcaggct ttgaggccga aggcaaaatt aagctcaaag acttcaatat
     3421 caagacagat ttaggtccag cttctcagga agtggatctg attatttcag tggaaggcgt
     3481 acagcagaag taagagtgaa atgttgccgg atggcacgca tccggcaatg atatttattc
     3541 tgttggatca ggaataccca gtgttgtatt cagacgtcca cgagacttat taaagatctt
     3601 attgccattt tcacgtcctg cccgacggcg tcgctgttct tctggcggta acgcgctttc
     3661 ttcgcagcaa atctcactac aacaaccttt gtatttttcc gcgcatactg gacactgaat
     3721 aaacagcagg tggcagccat catttttaca gttggtatgg ctgtcgcacg gcgcgccgca
     3781 ctggtggcaa tgcgcgataa tctcatccga tatacgttcg cccatccgct cgtcaaaaac
     3841 aaaatttttg ccaataaaac gcaccggcaa gccctgctcg cgcgccttac gggcgtattc
     3901 aataattccg ccctcgatat gccagacttt gttgaatccg ttatgtttca tccaggcact
     3961 ggccttttca caacgaatgc cgccggtgca gtacatgacg atttttttat ctttatgtgc
     4021 ctgcatcatc tcgactgctt ttggcagctg ctcacggaag gtatctgccg gaatttcgag
     4081 cgcgttttca aagtgcccca cttcatactc atagtggtta cgcatgtcga taaatagtgc
     4141 atcgggatcg tcaagcatgg cgttcacttc cgccgcttgc agatactcac caacattgct
     4201 ggcatcaaag tgaggatcgt caataccgtc ggcaacaatg cgatcgcgta ccttcatgcg
     4261 cagtacccag aaggatttcc cgtcatcatc caacgcgata ttcaggcgta aaccctctaa
     4321 agccgggtcg aaggcataga gctgcgcgcg aaatgtttca acattgctcg caggtacgct
     4381 gatttgcgcg ttaatgccct catgcgccag atacactcgc ccaaaaacat tcagcgcggt
     4441 aaacagctga tataaagcgt cacgggtcgc cttaggatcg gcgatgtgga aatacttgta
     4501 aaacgaaatg gtggttcgcg gttcgctctc agccaacatt ttggctttta acgcgtcgtt
     4561 ggaaatgcgg ttgtgtaaca ctggcatggt gtacggttcc tgcgagatgg gaaagtaaaa
     4621 atccgcggca tgatatagca attatcgata attaacatcc acacatttta cgctacattt
     4681 gcgcattaaa aattatttgt tatttacaag cgcggcaatt tcgcccagtc ttcagccaca
     4741 aattttggtt gcgggcgaaa aaatgcgaca atacatacaa ttgcccgtat aggttgaaaa
     4801 acaggattga tatgacgaat ctacccaagt tctccaccgc actgcttcat ccgcgttatt
     4861 ggttaacctg gttgggtatt ggcgtacttt ggttggtcgt gcaattgccc tacccgatta
     4921 tctaccgcct cggttgtgga ttaggaaaac tggcgttacg ttttatgaaa cgacgcgcaa
     4981 aaattgtgca tcgcaacctg gaactgtgct tcccggaaat gagcgaacaa gaacgccgta
     5041 aaatggtggt gaagaatttc gaatccgttg gcatgggcct gatggaaacc ggtatggcgt
     5101 ggttctggcc ggaccgccga atcgcccgct ggacggaagt gatcggcatg gaacacattc
     5161 gtgacgtgca ggcgcaaaaa cgcggcatcc tgttagttgg catccatttt ctgacactgg
     5221 agctgggcgc gcggcagttt ggtatgcagg aaccggggat tggcgtttac cgcccgaacg
     5281 ataatccact gattgactgg ctacaaacct ggggccgttt gcgctcaaat aaatcgatgc
     5341 tcgaccgcaa agatttaaaa ggcatgatta aagccctgaa aaaaggcgaa gtggtctggt
     5401 acgcaccaga tcatgattac ggcccgcgct caagcgtttt cgtcccgtta tttgccgttg
     5461 agcaggctgc gaccacgacc ggaacctgga tgctggcacg gatgtccggc gcatgtctgg
     5521 tgcccttcgt tccacgccgt aagccagatg gcaaagggta tcaattgatt atgctgccgc
     5581 cagagtgttc tccgccactg gatgatgccg aaactaccgc cgcgtggatg aacaaagtgg
     5641 tcgaaaaatg catcatgatg gcaccagagc agtatatgtg gttacaccgt cgctttaaaa
     5701 cacgcccgga aggcgttcct tcacgctatt aaatctccca tgccggatgc ttcagaatgg
     5761 catccggcat taccacagca aatccccctg atttagcgat aaaagctctc tggattgcgc
     5821 cccctggaag tcgggcgcat aattagtgtg tttatctttt cttcttatgt tcaccgcgcc
     5881 tggcgcacca acagcggatt gctatgtcac cctgtgaaaa tgacacccct ataaactgga
     5941 aacgaaacct gatcgtcgcc tggctaggct gttttcttac cggggccgcc ttcagtctgg
     6001 taatgccctt cttacccctc tacgttgagc agcttggtgt taccggccac tccgccctga
     6061 atatgtggtc cggtattgtc ttcagtataa tgggttccta aacacatcat tttagatatt
     6121 gaatatgaga cgggcaatca gctacatcag attttcaagt gaacgacaac tgaaagggga
     6181 ctctgtacgc cgacaatcta agctggttac tgactggctt gataagaacc ctgagtttta
     6241 cttggattca tccttgagct ttaaagattt ggggaaaagt gcttttagtg gtaagcacct
     6301 gaaaggtggt ctaggtgact ttttaaccgc tatcgagaaa gggcttgtta aagctggtga
     6361 tacgctacta atcgagagtt tagaccgtct gtcacgtcag gatattgata ttgcttctga
     6421 gctattacgc cgtatcttaa gggctggtgt agatgtagtc acactgagcg atggagaaca
     6481 ttacacacga gaaagtttaa aagacccgtt agccctgatt aaaagcattc tgataatgca
     6541 gcgggcgcat gaggaaagtt tgaggaagtc tgagcgtgtc caagctgcat ggaaccgtaa
     6601 aaaagaactc atctccgaag gtatcaaggt atctaggcgt tgtcctgcat ggttaaggct
     6661 gaatgatgac cgtcgaacat tcacaatcat tcctgataaa gtggaagttg ttaaacgagc
     6721 atttgacctg agattacagg gtttaagctt ttgggctatc accagaactt taaacgatga
     6781 aggtcacttg tcattgaatc aatacacacc caaacaaaag gggtggtctg atactgctgt
     6841 aaagaaacta ctcagaaatc gtgctgtgat tggatgtttc acacctgctg gacgtgaaga
     6901 agttcagggt tactaccccg caataatcag tgagtcacta ttctatcgag tacagcaact
     6961 gaacacaggg caatatggta gggcttctgt atcaagtaat ccgctatcag tcaacttgtt
     7021 caggggtatc atcaaatgtt ctgagtgtgg ggctactatt gttcttggtg gatatgcctt
     7081 aaaacgtttt ggaatgtaca gatgccctaa tcgttcagct aacaggtgta gtgcaaaggc
     7141 tatcagtcgt aagcaaacgg atgcaactat cctttacatg ctcgcattat gcgaccgttt
     7201 tgaaactgaa aatactgaca ctattgatag ccttaaatta cagcgtgaag atttg
//
LOCUS       DADLTF010000176         7220 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.178, whole genome shotgun sequence.
ACCESSION   DADLTF010000176 DADLTF010000000
VERSION     DADLTF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.178"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            26..877
                     /gene="hchA"
                     /locus_tag="JN749_003653"
     CDS             26..877
                     /gene="hchA"
                     /locus_tag="JN749_003653"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HAY7369914.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(985..2343)
                     /locus_tag="JN749_003654"
     CDS             complement(985..2343)
                     /locus_tag="JN749_003654"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HAY7369915.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2343..3014)
                     /gene="hprR"
                     /locus_tag="JN749_003655"
     CDS             complement(2343..3014)
                     /gene="hprR"
                     /locus_tag="JN749_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HAY7369916.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3147..3560
                     /gene="hiuH"
                     /locus_tag="JN749_003656"
     CDS             3147..3560
                     /gene="hiuH"
                     /locus_tag="JN749_003656"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HAY7369917.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3669..4673
                     /gene="msrP"
                     /locus_tag="JN749_003657"
                     /pseudo
     CDS             3669..4673
                     /gene="msrP"
                     /locus_tag="JN749_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4674..5308
                     /gene="msrQ"
                     /locus_tag="JN749_003658"
                     /pseudo
     CDS             4674..5308
                     /gene="msrQ"
                     /locus_tag="JN749_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5566..6216
                     /gene="zinT"
                     /locus_tag="JN749_003659"
     CDS             5566..6216
                     /gene="zinT"
                     /locus_tag="JN749_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HAY7369918.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMISIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2045 a         1634 c         1513 g         2028 t
ORIGIN      
        1 actaagcaac aataaggaat acactatgac tgttcaaaca agtaaaaacc cgcaggtcga
       61 tattgctgaa gataatgcgt tcttcccttc agaatattcg cttagccaat atacgagtcc
      121 cgtctctgat cttgatggcg tggactatcc aaaaccgtat cgcggtaaac ataaaattct
      181 ggtgatcgcc gcggacgaac gttatttgcc gaccgataac ggaaaactgt tctcgaccgg
      241 taaccatccg attgaaacgt tgctgccgtt gtatcatctc catgctgcag gtttcgaatt
      301 cgaagtggcg accatttccg gtctgatgac caagtttgaa tactgggcta tgccgcacaa
      361 agatgaaaaa gtaatgccat tctttgaaca gcataaatcg ttattccgta atccgaagaa
      421 actcgcggat gttgtcgcca gcctcaacgc tgatagcgaa tatgcagcaa tctttgttcc
      481 tggtgggcat ggcgcactta ttggtttacc agaaagccag gacgtggctg ccgctttaca
      541 gtgggcaatc aaaaatgacc gttttgttat ctccctttgc cacggtccgg cggcttttct
      601 ggcacttcgc cacagcgata acccactgaa tggttattct atttgcgcat tcccagacgc
      661 cgcagacaaa caaacgccag atattggcta tatgccgggt catctcacct ggtacttcgg
      721 cgaagagttg aagaaaatgg gcatgaacat cattaatgac gacatcaccg ggcgagtaca
      781 taaggaccgt aaacgtctca ccggcgacag tccttttgca gcgaatgcgt tgggtaaact
      841 ggcggcgcag gaaatgctgg cagcttacgc gagttaatcc ctgttacatt gtgatgtaag
      901 cgcataatca atcgcagcca cgttaatact cacgacgtgg ctgcgttttt tcacggttaa
      961 cttatggcgt actgaagccc gatgttaatt tctttgcggt aacgtaattc ggaacacgtt
     1021 atgcttactg agatagtgat acgtagcact tcctccatgt aattcggcaa tcgctttgac
     1081 taaagaaagg ccaagtccct gacctacgga atgacgcgaa ttatctcccc gccaaaatct
     1141 acggaagagt ttttcaggct cattaatttt cgttccagga ctggcgatat caatattaag
     1201 agagccgttg gcatcaagaa aactggttat atgaatacgc gatttttctg gtgaatatcg
     1261 aatggcgtta acaataagat tcgataacat tcgttgcagt aaaattttat ccgcaaagat
     1321 ttgttgattg cacttgacct taaagcaaat ctctttctcg tctgaaagag attcaagata
     1381 gtccaaaaga ttttcaactt ccttattgag agaaagcgaa tccagtttca ccaaaacatt
     1441 gtttttgtct gccctggcaa gaaacagtat attctctgtt aaccgcgaaa tattttccag
     1501 ctcttcaatg tttccggcaa ttgttttttg atattcagcg atacttctga tttgactgag
     1561 cgtaacctga ttctgaccca gtaatgcatt aattggcgtt ctaagttcat gagcgagatc
     1621 gtcagcaaat tgactaaggc gctcaaaatc tttgactaaa gcatgatgca ttttattcaa
     1681 cgcctgccca agaggtttta gttctctcgg taatgcgtta acctcaaccg gcactcggct
     1741 atcgttataa ttcagcgctt ccgttacgcc actcaacttt ttgatctctc gcaaccccgt
     1801 cctgattaac aacggactta atactgagca cagtataatg gcgacaatgc aaattataat
     1861 gctattaatt ttatactgtt caagcatatt atgtctggct gaagccaatt tagccacagt
     1921 aaccgttaat gatggcgaaa cttcatcaat gttaattcgt aaagcatcta tctctgtatc
     1981 attaataatg cttctgtaaa tgcctgctgc gctgattgtc tcactagcag gtatgttatt
     2041 taacatgtca tcactgacat ttgtccggtt aacaatttta ttgatgccat cactatgaat
     2101 gatcaaaata tcctgactaa catccatcat ccggttaaag tacacaggta acgtatctgg
     2161 atttacccca tcaattaaca actgcttgat ctgcgctgtc cggttaatga gtgttgtatc
     2221 atcgcgccat ttcaactcac ttgccaggcc attatagaga gtccatacaa ttccagcgcc
     2281 agcaacagac tgcagaaata taaaaagcaa ggttaaacgg acggttatag ataatctttt
     2341 cattattttt ttaccgctac gaatgaatag cccatccccc ggattgtggc gattagcttt
     2401 tcaggaaaag gatcatcaac ttttgcgcgg agcctgcgaa tggcgacatc caccgtattg
     2461 gtatcactat caaagttgat tccccaaatt tcactcgcaa taaccgttct gggtataatc
     2521 tcgccagctc tggaggccag tagccaaagt aactgaaact ccttgcgcgt cagtgtaata
     2581 ctgatattgt ccctgctgac actttgactg acagagtcca tttttaagcc actgatttct
     2641 aatgttgaat tcagagcgtg atgttgcctt aattgtgccc gaacccttgc taacaactca
     2701 gaaaatgaaa aaggttttac cagataatca tttgccccac tgtccaaccc tctgactctg
     2761 tcatcgacag aatcccttgc agtaaggcaa ataacagggg tttgctttgc cgttcttaac
     2821 gtttgtaaga tctgccagcc atccataccc ggaagcataa tatccagaat gatcaatgca
     2881 taatcatcct tcagcgcaag ataaagccca tctctgccat cagaaacggc atcaatgaca
     2941 taacccgctt cggaaagccc ctgcgttacc cattcctggg tcctttgatt atcttcaata
     3001 agtagaatct tcatgaaaat aatatgccat aaatcatatg ttatagaaac aacctggttc
     3061 attacaaaat tgtaatgctg ctgtaaggtt accctggacg ttttttcgct atcctcaaaa
     3121 ctcattcaca tgacaaggat ataaacatgt taaagcgtta tttagtactc tcggtagcaa
     3181 cggcagcatt ttcattacct tctttggttt atgccgcaca acaaaacatt cttagcgtgc
     3241 acattttgaa ccagcaaaca ggaaaacccg ctgccgacgt gacagtcact cttgaaaaga
     3301 aagcggataa cggctggtta caacttaata ccgccaaaac agataaggat ggacgaatta
     3361 aggcactgtg gcccgagcaa actgcgacta ctggcgatta ccgtgtcgta tttaaaaccg
     3421 gggactattt caagaaacaa aatcttgaaa gtttcttccc tgagattccc gttgaatttc
     3481 atattaataa agtgaacgag cattatcatg tgcctttgtt actcagccaa tatgggtatt
     3541 caacctatcg tggcagttaa tgcgtaaata tgtaaaagct gatctctgct actgttaggg
     3601 aggtgactcc ctgacagatt aacagtaaac agctcttgtt ggttaacgac aaaaaagtgt
     3661 gatggcttat gaaaaagaat caatttttaa aagaatcaga tgttacggcc gagtcggtat
     3721 tctttatgaa gcgtcggcag gtgttaaaag cactgggcat cagcgcagct gcattttctt
     3781 tgcctcacgc tgcgcatgcc gatctgctta gctggtttaa agggaacgat cgtccgcccg
     3841 cccccgccgg aaaaccgctg gagttcagca agcctgccgc ctagcaaaat aacctgccac
     3901 tgacgccagc agataaagtc tccggttata acaacttcta tgaattcggg ctggataaag
     3961 acgatcccgc ggctaatgct ggtagcctga aaaccgatcc atggacactg aaaatcagcg
     4021 gcgaagtggc aaaaccattg accctcgatc atgatgattt aacccgtcgc ttcccgctgg
     4081 aagagcgtat ttatcgtatg cgctgcgtgg aagcgtggtc gatggtggtg ccgtggattg
     4141 gttttccgct acacaaattg ctggcgcttg ccgaacccac cagcaatgcg aagtatgtcg
     4201 ctttcgaaac gatttatgcg ccggaacaga tgcctggcca gcaggaccgc tttatcggcg
     4261 gcgggctgaa atatccttat gtcgaaggat tgcgtctcga cgaagcaatg catccgctca
     4321 cactgatgac cgtaggtgtt tatggcaagg cgttaccacc gcaaaatggc gcgccggtgc
     4381 gactgattgt gccgtggaaa tatggcttta aagggattaa atcgatcgtc agtattaagc
     4441 tgacccgcga gcgtccgcca accacctgga atctggcagc gcctgacgaa tacggttttt
     4501 acgccaacgt taatccgcat gttgatcacc cgcgctggtc acaggctacc gaacgattta
     4561 ttggttcagg cggcattctc gatgttcagc gccagccaac actactgttt aatggttacg
     4621 ccgaccaggt ggcatcgctg tatcgtggcc tggatttgcg ggagaatttc taaatgcgtc
     4681 tgacagcaaa acaggtgata tggctgaaag tttgcctgca tcttgccgga ttgttgccgt
     4741 ttctctggct ggtctgggcg atcaatcacg gtggactggg tgccgatccg gtgaaagata
     4801 ttcagcattt taccggtcgc actgcactga aatttttgct tgcggccttg ttaatcacac
     4861 ctctggcacg ctacgcaaaa cagccgttat tgatacgcac tcgccgcctg ttaggcttat
     4921 ggtgctttgc ctgggcgaca ctgcatttaa ccagttacgc attgctggag ttgggcgtga
     4981 ataatctggc gttattaggt aaggagttaa ttacccggcc ttatttaacg ttaggaatta
     5041 tcagctgggt tattttgctt gctttagcgt tcacttcaac ccagtcgatg cagcgaaaac
     5101 tcggcaaaca ctggcaacag ttgcataact tcgtctatct tgtcgcgatc ctggccccaa
     5161 tacattatct gtggtcagtg aagattattt caccgcagcc actcatctac gctgggctgg
     5221 ctgtactgct ttagccttac ggtataagaa gttgctttcc cttttcaacc ggttacgcaa
     5281 gcaggcccac aacaaattat ctctataact tcatattgcc gacaaacttc gcgttatccc
     5341 ggccggatgc ggcgcgagcg ccctatccgg cccacacaga tcgcatcaag ttatgtttcg
     5401 tttgtcatca acctttcatc accagaataa tttattttat ttcaataaga tagttcaata
     5461 cacctgagaa taccatgctc tcatttccta atgagttgtt gcattttttc atatgttaca
     5521 atataacatt acacataatg tacattaact ctggaggaaa ttgttttggc gattcgtctt
     5581 cataaactgg ctgttgcttt aggtgtcttt attgttagcg ctcctgcctt ttcgcatggt
     5641 catcattctc atggcaaacc cttaacagag gtcgaacaaa aagctgctaa tggtgttttt
     5701 gatgatacca atgtacaaaa ccgaacgctc agtgactggg atggagtctg gcaatccgtt
     5761 taccctttac tgcaaagtgg caaacttgat cccgtctttc agaagaaagc ggatgcagat
     5821 aaaaccaaaa catttgctga aattaaagat tattatcgca aaggttatcc gacagatatc
     5881 gaaatgatta gcattgagga cggcattgtc gaattccata gaaataatga aacaacatcc
     5941 tgtaaatatg attacgatgg atacaaaata ctcacctata aatcaggcaa gaaaggcgtt
     6001 cgttacttat tcgaatgtaa agatcctgaa agcaaagccc ctaaatatat acaatttagc
     6061 gatcatataa ttgcaccacg aaaatcatct cattttcaca tttttatggg taatgattcc
     6121 cagcaatctt tattgaatga aatggaaaac tggccaacgt attatcccta tcagttgagt
     6181 agcgaagaag tggtcgagga gatgatgtct cattgatttt tttgcgctga cttcagttat
     6241 ttaacagaac agtgcctggt agtaacaaca ataatttacc aagacaaata agtaagacaa
     6301 actcagacac gctcatactt gagtcgtgtc ttttttatta tgtctgtaga aaaaaactga
     6361 gaaaaaggcg aacaataaat acacaactca gccatacaga ctggcagcgc aaatgtttcc
     6421 aggagcaagt catctgtcat ggtaaagcta accagaaata taggttaatt ttgttgttta
     6481 aacctttttt acttgttagt ataatacaaa ggctttcaaa aaagctggga aattcaacaa
     6541 ctcttggaag ttatggactt tgtcccgcgg ctttaatctg acggccgcgt tccttttttg
     6601 gctcaattat tgaccccttc tctacgcaac ttcagttccc gccagcaact ttgcggcagt
     6661 ttcataggat caatgtctaa aagaataatg gtgaccgata agaaaacgac tgaataactg
     6721 tagattttcg ctcgaaacct tcctgtcagc tccatagcga atcaagcgct gaatgtcaca
     6781 gtatcgaaca gaaaacagtg acgatctaac ccttcaagaa tattctacga ttgttctgtt
     6841 taggaaaagc aaggcgggaa gtcgggagat aagtcattga taaagtggcg gagagagggg
     6901 gatttgaccc cccggtagag ttgcccctac tccggttttc gagaccgatg atacagagtc
     6961 gaaaaatcaa agagttaaaa atttttcttg gaaaaaatgt cttaaaatcg tacacgaaaa
     7021 atcaatgaat tacataacat taaagaagaa tattccaagc cttcttgagt ccaccacctg
     7081 ttttcaggtg tcattcagac gtgatttgcc ccagattggg aaagcacgct tttttctcag
     7141 cttattccgt tttgcctgtg tgttgaataa ttccactgca gttatataaa atcggtaacg
     7201 gctggaaatc attcaatact
//
LOCUS       DADLTF010000177         7041 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.179, whole genome shotgun sequence.
ACCESSION   DADLTF010000177 DADLTF010000000
VERSION     DADLTF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.179"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..731
                     /locus_tag="JN749_003660"
     CDS             <1..731
                     /locus_tag="JN749_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7369919.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPSVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMIQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIAWAITTQQKEYQA"
     gene            complement(1034..1804)
                     /locus_tag="JN749_003661"
     CDS             complement(1034..1804)
                     /locus_tag="JN749_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369920.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="JN749_003662"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="JN749_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="JN749_003663"
     CDS             complement(2295..2516)
                     /locus_tag="JN749_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369921.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="JN749_003664"
     CDS             complement(2517..3200)
                     /locus_tag="JN749_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HAY7369922.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="JN749_003665"
     CDS             complement(3264..3575)
                     /locus_tag="JN749_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369923.1"
                     /translation="MKYDGDGRATARFFSDKGCRRVPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3572..4543)
                     /locus_tag="JN749_003666"
     CDS             complement(3572..4543)
                     /locus_tag="JN749_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HAY7369924.1"
                     /translation="MRHGLMEAACERRIPMPNWCSNRMYFPGEPAQIAEIKRLASGAV
                     TPLYRRATNEGIQLFLAGSAGLLQITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTH
                     LQNGVLLDEQNCLMLHELWLQSGTGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNE
                     DVSVWWNRLCDNVVPEKTMPFDLLTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYG
                     VKWPCGYDLNISSQGENFIQVDFDTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFC
                     GWQLYERGELVDVLWGELEWSSPTDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="JN749_003667"
     CDS             4914..5873
                     /locus_tag="JN749_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7369925.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="JN749_003668"
     CDS             5873..6268
                     /locus_tag="JN749_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HAY7369926.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="JN749_003669"
     CDS             6451..6831
                     /locus_tag="JN749_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAY7369927.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6877..>7041
                     /locus_tag="JN749_003670"
     CDS             6877..>7041
                     /locus_tag="JN749_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7369928.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLP"
BASE COUNT         1915 a         1828 c         1709 g         1589 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctagtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat acagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctgggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcacag gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta gcggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccgcaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgccctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc gtccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acgccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga acacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca tcgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggcgtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acacagatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccagcgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggttca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaagt agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgctt
     4801 gacatatact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 tataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gacccccgac tgttaacgct
     6061 aatcagcggt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaataacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcccctgtta cctcctgaac ccgccacacc acgggccgga
     6961 cgcccatggg ctgagcaccg taaaatcatc aatggcatgt tctgggtgtt atgttccggt
     7021 gcaccatggc gcgatttacc c
//
LOCUS       DADLTF010000178         6970 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.180, whole genome shotgun sequence.
ACCESSION   DADLTF010000178 DADLTF010000000
VERSION     DADLTF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.180"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1143
                     /locus_tag="JN749_003671"
     CDS             <1..1143
                     /locus_tag="JN749_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HAY7369929.1"
                     /translation="SYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVF
                     DNVMVNEVETAYLMQGLWHSKFIACQAGTCRVGLHFLGQCVSVSVSSCHFSRGNYSAD
                     ESFGIRIQPQTYAWSSEAVRSEAIILDSETMCIGFKNAVYVHDCLDLHMEQLDLDYCG
                     STGVVIENVNGGFSFSNSWIAADADGTEQFTGIYFRTPTSTQSHKIVSGVHINTANKN
                     TAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNIVDINECVSFDKCIFNKPLRYLRSGG
                     VSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSVNGIINVPVAVGATSGSAAIPNPGNL
                     TYRVRSLFGDPASSGDKVSVSVVTINVTRPSPVGVALPSMVEYLAI"
     gene            complement(1177..2178)
                     /locus_tag="JN749_003672"
     CDS             complement(1177..2178)
                     /locus_tag="JN749_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7369930.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(2334..2417)
                     /locus_tag="JN749_003673"
                     /pseudo
     CDS             complement(2334..2417)
                     /locus_tag="JN749_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2732..3202)
                     /locus_tag="JN749_003674"
     CDS             complement(2732..3202)
                     /locus_tag="JN749_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HAY7369931.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            complement(3180..4469)
                     /gene="bioA"
                     /locus_tag="JN749_003675"
     CDS             complement(3180..4469)
                     /gene="bioA"
                     /locus_tag="JN749_003675"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HAY7369932.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            4556..5596
                     /gene="bioB"
                     /locus_tag="JN749_003676"
     CDS             4556..5596
                     /gene="bioB"
                     /locus_tag="JN749_003676"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HAY7369933.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            5593..6747
                     /gene="bioF"
                     /locus_tag="JN749_003677"
     CDS             5593..6747
                     /gene="bioF"
                     /locus_tag="JN749_003677"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HAY7369934.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            6734..>6970
                     /locus_tag="JN749_003678"
     CDS             6734..>6970
                     /locus_tag="JN749_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAY7369935.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQ"
BASE COUNT         1795 a         1730 c         1803 g         1642 t
ORIGIN      
        1 tcttatgatt cagacactat cggccgttat ctgagaaata tacgagtaac tggtaataac
       61 acctgcaatg gtattgacac aaacattaca gcagaagact ctgtcatcag acaggtttat
      121 ggctgggtat ttgataatgt aatggtgaat gaagttgaaa ccgcttattt aatgcaaggg
      181 ctctggcact caaaatttat agcatgtcag gctggaacct gtagagtcgg tcttcacttt
      241 ttaggccagt gcgtaagtgt tagtgtcagc tcctgccatt tcagcagagg aaattattct
      301 gctgatgaaa gctttggcat caggattcag cctcaaacgt atgcgtggtc gtcagaggca
      361 gttaggtcag aagcaataat tttagacagt gagaccatgt gcattggttt taaaaatgcc
      421 gtctatgttc atgattgcct tgatttgcat atggaacaac tggatttaga ttattgcggt
      481 tcaacaggcg tggttataga gaatgtaaac ggaggatttt ctttctcaaa ctcatggata
      541 gcagcagatg ccgatggcac tgaacaattt acggggatat attttagaac gccgacctca
      601 acgcagtcac ataaaattgt cagtggtgtt catatcaaca ctgcaaacaa aaacacggct
      661 gcaaacaatc aaagtatagc gatagaacag tcggcgatct tcgtctttgt aagtggttgt
      721 acgttaactg gtgatgaatg ggctgtaaat attgtcgaca tcaatgaatg tgtttctttc
      781 gataagtgca tattcaataa gcctctacgc tatcttcgta gcggtggcgt gtcagttact
      841 gactgttatt tggctggcat taccgaggtg cagaaaccgg aaggcagata caatacgtat
      901 cgtggctgtt caggcgtacc gtctgtaaat gggatcatta atgtcccagt tgcggtaggt
      961 gccacaagcg gatctgcagc tataccgaac ccggggaacc tgacatacag agtaagaagc
     1021 ctttttggtg accctgcatc aagtggtgac aaggttagtg tttccgtggt gacaattaat
     1081 gttactcgcc caagcccagt aggagtcgcg ctgccttcaa tggttgagta tctggccatc
     1141 tgattatttt aggggccata tggccccttt tattgttcaa tccagggata atttaggcga
     1201 acttcgtgtc aggaatcttt tttcaaccag attccatgaa agatgggaca agaaaagaac
     1261 agtcacggca cttaacaaca ttgacgggta aaaacccatg tgcaatgtgt ttataacaac
     1321 ctgctgaacc gggaatgcat aaatgtacac accatacgag taatcaaacc tgcccttaac
     1381 cagtgggtcg ccaacggatg tgcatatggc tattgtcgag aatgaaacca ggatatagca
     1441 agtcatagta taatcaatgc ctttgccata actagcgtag gcgtacatag caagcaaaga
     1501 aactacagta attttaacat tacttacatt ccatgacttc tcatacattg ccatagtggc
     1561 accaaaaaag aacgccagtc cacgcaaagg atacagccat aacggaatag agaacattac
     1621 atccctgttt tctgacacac ttccaattaa tgaaagagaa acaaaaacca acagtataac
     1681 tatgaatgct tttccgttct ttagtagggc aactgctact cccgttatta tataacacag
     1741 gaactcaagc ggtagggtcc acaaactgcc gttaattccg gcatgaatta aatgtgatgt
     1801 aatgtcagca tctggagcct gactcataaa aatagagctt atggtcttcc tgacaatgtc
     1861 atggctaaaa tattctgcgc tgaagtcgtt aagtatccat ccaaataaaa aatatgtcag
     1921 tatagagcaa gggaccagcg ccgggaatat tcttctcgct cttttagcca tgaaatcaat
     1981 aaaggaatca ctcctgatag ctgattttga aatcagatat cctgatattg aaaagaatat
     2041 gataactgca atgccgccag cactttcaaa gccgaacagg tacggctccg gctgcccaga
     2101 gagcgcgtag tggtgactga ccagaaccat catagctgcc acaagccttg ccgtgtcaaa
     2161 gcagttgctc ttatgcatta aaataaagcc tctaagtttt tgcgaggaaa aatatcagat
     2221 ctttctgtca tagtccattt tgggtccagc cagtgaggtc atgctcgcgt tgaggccaaa
     2281 gaatgagcat gggctcaggt agagggcaag gctctgctca ggcatggatt tgcctaccta
     2341 accttctcat caagccaatc cgcccaccac tgcatcattt ctctgcgctt atcgagatac
     2401 ttagcatggt tgtaaattca actaacccag tccgttgaca aaaaattagc gcaagaggac
     2461 aaaaaatcac cttgcgctaa tgctctgcct caggtcacta atactatcta agtagttgat
     2521 tcatagtgac tggatatgtt gtgttttgta gtattatgca gtctattatt tagactaaat
     2581 ctttcataac atattgatat ttacagcgtt ttatgtttct cgttcagctt ttttatacta
     2641 acttgagcga aacgggaagg taaaaagaca aaaagttgtt tttaatacct ttaagtgata
     2701 ccagatggca ttgcgccatc tggcagagtg attaactaaa catcgcggta atcgatgcgc
     2761 ttgccagaga gtggaaatga acgttaaacc cgaccatcgc gccgctgcct ccttcatcga
     2821 catcaatacg ctctacatcc agcgcgtgaa cggtaaaaat gtagcgatgg gtttcgcctt
     2881 tcggcggcgc tgcgccatcg tatccggttt taccaaaatc ggtacgcgtc tgcaaaacgc
     2941 cgtctggcat tgctaccaga ccagagccaa acccttgcgg taatacgcgg gtatcagcgg
     3001 gtaaattaac aactacccag tgccaccagc cggagccggt tggcgcatcc gggtcgtagc
     3061 aggtgacaac aaaacttttc gttcccgcag gaacatcatc ccacgccaga tgcggtgaaa
     3121 tattatcgcc atcgtaaccc atgccgttaa agacatgacg atgcggcaac ttatcgccat
     3181 tattggcaaa aaaatgtttc atcctgtacc gcgcggttaa ccgctgcggt cagacgctgc
     3241 aactgttgcg ggagaataat atagggcggc atcaggtaaa tcagtttgcc aaaaggccgg
     3301 atccataccc cctgttcgac aaagaatttt tgcagcgccg ccatattcac cggacgagtg
     3361 gtttcgacca caccgattgc ccccagtacg cgcacatcgg caaccatttc ggcatcacgg
     3421 gctggtgcca gttgctcgcg cagctgcact tcaatagccg ccacctgctg ctgccattcg
     3481 ccggattcga taatcgccag gctggcgttt gctgccgcgc aggccagcgg attgcccata
     3541 aaagttggcc catgcataaa gcagccggct tcgccgttac tgatggtttc tgcaacctcg
     3601 cgcgtggtaa gtgtggcgga aagggtcatt gtgccgccgg ttaaggcttt accgaggcac
     3661 aaaatgtccg gcgcgatttc tgcatattca caggcaaaca gtttgccggt acgaccaaat
     3721 ccggtggcga tctcgtcggc aatcagcaag ataccttcgc gatcgcacat tttgcggatt
     3781 cgttttaacc attccggatg gtacatgcgc atcccgcctg cgccctggac aatcggctca
     3841 atgatcaccg ctgcgatttc atgacgatgc gccgccatca ggcgggcaaa accaaccata
     3901 tcgcgttcat cccattcacc atccatgcgg ctttgcgggg cgggagcaaa caagttttct
     3961 ggcagatagc ctttccacag gctgtgcatt gagttgtccg gatcgcacac cgacatcgcg
     4021 ccaaaggtat cgccatgata accattgcgg aaggtcagaa aacgctggcg cgcttcgcct
     4081 ttggcttgcc agtactgcaa cgccattttc atcgccactt ccaccgctac ggaaccggag
     4141 tccgcgagaa aaacgcactc cagcggttgc ggcgtcattg ccaccagttt gcggcatagc
     4201 tcgatggcgg gcgcatgggt gataccgcca aacatcacat gcgacatggc atcaatttgc
     4261 gtcttcatcg ccgcattaag ctgcgggtga ttgtagccgt ggatcgccgc ccaccaggac
     4321 gacataccgt caaccaggcg tctgccgtca gacaaaatta gctcgcaacc ttcggcgctc
     4381 gccaccggat aaaccggcag aggggaggtc atggatgtgt atgggtgcca gatatggcgt
     4441 tggtcaaagg caagatcgtc cgttgtcata atcgacttgt aaaccaaatt gaaaagattt
     4501 aggtttacaa gcctacaccg aattaacaac aaaaaacacg ttttggagaa gccccatggc
     4561 tcaccgccca cgctggacat tgtcgcaagt cacagaatta tttgaaaaac cgttgctgga
     4621 tctgctgttt gaagcgcagc aggtgcatcg ccagcatttc gatcctcgtc aggtgcaggt
     4681 cagcacgttg ctgtcgatta agaccggagc ttgtccggaa gattgcaaat actgcccgca
     4741 aagctcgcgc tacaaaaccg ggctggaagc cgagcggttg atggaagttg aacaggtgct
     4801 ggagtcggcg cgcaaagcga aagcggcagg atcgacgcgc ttctgtatgg gcgcggcatg
     4861 gaagaatccc cacgaacgcg atatgccgta cctggaacaa atggtgcagg gggtaaaagc
     4921 gatggggctg gaggcgtgta tgacgctggg cacgttgagt gaatctcagg cgcagcgcct
     4981 cgcgaacgcc gggctggatt actacaacca caacctggac acctcgccgg agttttacgg
     5041 caatatcatc accacacgca cttatcagga acgcctcgat acgctggaaa aagtgcgcga
     5101 tgccgggatc aaagtctgct caggtggcat tgtgggctta ggcgaaacgg taaaagatcg
     5161 cgccggatta ttgctgcaac tggcaaacct gccgacgccg ccggaaagcg taccaatcaa
     5221 catgctggtg aaggtgaaag gcacgccgct tgccgataac gatgacgttg atgcctttga
     5281 ttttattcgc accattgccg tagcgcggat catgatgccg acctcttacg tgcgcctttc
     5341 tgccggacgc gagcagatga acgaacagac tcaggcgatg tgctttatgg caggcgcaaa
     5401 ctcgattttc tacggttgca aactgctgac cacgccgaat ccggaagaag ataaagacct
     5461 gcaactgttc cgcaaactgg ggctaaatcc gcagcaaact gccgtgctgg caggggataa
     5521 cgaacaacag caacgtcttg gacaggcgct gatgaccccg gacaccgacg aatattacaa
     5581 cgcggcagca ttatgagctg gcaggagaaa atcaacgcgg cgctcgatgc gcggcgtgct
     5641 gccgatgccc tgcgtcgccg ttatccggtg gcgcaaggag ccggacgctg gctggtggcg
     5701 gatgatcgcc agtatctgaa cttttccagt aacgattatt taggtttaag ccatcatccg
     5761 caaattatcc gtgcctggaa gcagggggcg gagcaatttg gcatcggtag cggcggctcc
     5821 ggtcacgtca gcggttatag cgtggtgcat caggcgctgg aagaagaact ggccgagtgg
     5881 cttggctatt cgcgggcgct gctgtttatc tctggttttg ccgcgaatca ggcagtcatt
     5941 gccgcgatga tggcgaaaga ggaccgtatt gttgccgacc ggcttagcca tgcctcattg
     6001 ctggaggctg ccagtttaag cccgtcgcag cttcgccgtt ttactcataa cgatgtcgct
     6061 catctggcgc gactgcttgc ttccccctgt ccggggcagc aactggtggt gacagaaggc
     6121 gtgttcagca tggacggcga tagcgcgcca ctggcggaaa tccagcaggt aacgcaacag
     6181 cacaatggct ggttgatggt cgatgatgcc cacggcacgg gcgttatcgg ggagcagggg
     6241 cgcggcagct gctggctgca aaaggtaaaa ccagaattgc tggtagtgac ttttggcaaa
     6301 ggatttggcg tcagtggggc tgcggtgctt tgctcaagta cggtggcgga ttatctgctg
     6361 caattcgccc gccaccttat ctacagcacc agtatgccgc ccgctcaggc gcaggcatta
     6421 cgtgcgtcgc tggcggtcat tcgccgtgat gagggggatg cacggcgcga aaaactggtg
     6481 tcactcattg cgcgttttcg tgccggagta caggatttgc cgtttacgct ggctgattca
     6541 tgcagcgcca tccagccatt gatcgtcggt gataacagcc gtgcgttaca actggcagaa
     6601 aaactgcgcc agcaaggctg ctgggtcacg gcgattcgcc cgccaaccgt acccgctggt
     6661 actgcgcgac ttcgcttaac gctaaccgct gcgcatgaaa tgcaggatat cgaccgtctg
     6721 ctggaggtgc tgcatggcaa cggttaataa acaagccatt gcagcggcat ttggtcgggc
     6781 agccgcacac tatgagcaac atgcagatct acagcgccag agtgctgacg ccttactggc
     6841 aatgcttcca cagcgtaaat acacccacgt actggacgcg ggttgtggac ctggctggat
     6901 gagccgccac tggcgggaac gtcacgcgca ggtgacggcg ttagatctct cgccgccaat
     6961 gcttgcgcaa
//
LOCUS       DADLTF010000179         6802 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.181, whole genome shotgun sequence.
ACCESSION   DADLTF010000179 DADLTF010000000
VERSION     DADLTF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.181"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            94..1518
                     /gene="sbcB"
                     /locus_tag="JN749_003679"
     CDS             94..1518
                     /gene="sbcB"
                     /locus_tag="JN749_003679"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HAY7369936.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1561..1788)
                     /locus_tag="JN749_003680"
     CDS             complement(1561..1788)
                     /locus_tag="JN749_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HAY7369937.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="JN749_003681"
     CDS             complement(1802..2860)
                     /gene="yeeE"
                     /locus_tag="JN749_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HAY7369938.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALILAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVGAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="JN749_003682"
     CDS             complement(3039..4397)
                     /gene="plaP"
                     /locus_tag="JN749_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HAY7369939.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="JN749_003683"
     CDS             complement(4387..4467)
                     /gene="yoeI"
                     /locus_tag="JN749_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HAY7369940.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(4665..5594)
                     /locus_tag="JN749_003684"
     CDS             complement(4665..5594)
                     /locus_tag="JN749_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7369941.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(5640..6464)
                     /locus_tag="JN749_003685"
     CDS             complement(5640..6464)
                     /locus_tag="JN749_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAY7369942.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="JN749_003686"
     CDS             complement(6547..6801)
                     /gene="yoeB"
                     /locus_tag="JN749_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HAY7369943.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1839 a         1782 c         1595 g         1586 t
ORIGIN      
        1 tatttgacgt tcgtagacat actcaggtga aaaaagggtt gcttttcgag ttataacaga
       61 cagttaatta actacagtgg acaatgctag acaatgacag acacagataa acaacctacc
      121 ttcctctttc acgattacga aacctttggc acgcaccccg cgttagatcg ccctgcacag
      181 ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg gcgaacccga agtcttttac
      241 tgcaagcccg cggatgacta tttaccccgg cctggagcag tattaattac cggtattacc
      301 ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt ttgccgcccg tattcactcg
      361 ctttttaccg taccgaagac ctgtattctg ggctacaaca atgtgcgttt cgacgacgaa
      421 gtcacacgca acgtttttta tcgtaatttc tacgatcctt acgcctggag ctggcagcat
      481 gataactcgc gctgggattt actggatgtt atgcgtgcct gttatgccct gcgcccggaa
      541 ggaataaact ggcctgaaaa tgatgacggt ctaccgagct ttcgccttga gcatttaacc
      601 aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga tggctgatgt gtacgccact
      661 attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc tgtttgatta tctctttacc
      721 catcgtaata aacacaaact gatggcgttg attgatgttc cgcagatgaa acccctggct
      781 cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca gctgggtggc accgctggcg
      841 tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt tggcaggaga catttcgcca
      901 ttactggagc tggatatcga cacattgcgc gagcgtttat ataccgcaaa agccgatctt
      961 ggcgataacg ccgccgttcc ggttaagctg gtgcatatca ataaatgtcc ggtactggcc
     1021 caggcgaata cgctacgccc ggaagatgcc gaccgactgg gaattaatcg tcagcattgc
     1081 ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc gtgaaaaagt ggtagcgata
     1141 ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg atgcacagct ttataatggc
     1201 tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc tggaaaccga gccgcgtaat
     1261 ttaccggcac tggatatcac ttttgttgat aaacgaattg aaaagttgtt gttcaattat
     1321 cgggcacgca acttcccggg gacgctggat tatgccgagc agcaacgctg gcaggagcac
     1381 cgccaccagg tcttcacgcc agagtttttg cagggttatg ctgatgaatt gcagatgctg
     1441 gtacaacaat atgccgatga caaagagaaa gtggcgctgt taaaagcact ttggcagtac
     1501 gcggaagaga tcgtctaatt tgaagccgcc gccgctggta cggcggagga caatgttgag
     1561 tcaggctttt tgaacggtga tgctccacgc tgcatcgcca atctgctgat aatcggtgat
     1621 ggcatgtcct tcttctgccg cccactgtgg gattgcttca gttgcctggg tacaatcaaa
     1681 ctcaattacc aactcatcgc cgctggccat ttctgccagt gctgcttttg cttctatgag
     1741 tggaaacgga cagacctgcg tcaccacatc cagcttttta atcgccatct ttacacctta
     1801 cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg cggtcgaaca tacacaagcc
     1861 aggacgcagt ccagactccg agaatcataa atacaaggcc aatccagccc tgccaggtca
     1921 tcattgcagt cataaccagt ccattaccga tagagcaacc acctgcaata ctggcaccga
     1981 agcccatcag tacaccacct aacccgctac gtaatgttgt ttgtgcatca gctgcgcgaa
     2041 cgcggaactc acggctcgct ttggctgcaa taaatgcccc cacgaagatc cctaacacca
     2101 ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac cagaaattgc agaatattgg
     2161 ccgttgggga agtgactcct aacccaaaca tgcgtccggt tgcttcactc aggggccagg
     2221 ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa gggatgccag cgtttttcaa
     2281 acagaatatg agcgatcccg gttcggcgcg gcggtaaggt cgcgactttg agttttggct
     2341 tcttcaactc tttcatcacc acccagagcg ttatcaccag caaaacggta accaacggcc
     2401 acacagacaa attaaaagtc tcagcaatag agttatgttc agtagtgtag tgctgcaagg
     2461 tttgatttaa accactggcg tgtggagaac gcatcaccgc actcatcacc atataagtga
     2521 aaagcgcgat ccaactgccg atcaatccct cacccgcgcg ataccaggtc ccggtggcac
     2581 atccacccgc cagaacaatt cccagcccga aaagataccc acctataaca gtaccaagcc
     2641 acgggaacgc cccggcttcg taagtcagta ggcccgccag aattaacgca aaaaccccca
     2701 cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat gcgattattt ttcgcgatat
     2761 acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg ctgcatgaca aatcccagca
     2821 gagcaccaca aattagcccg cttaatatca ttgaaaacat aaattaatta accagatgaa
     2881 tgttaatggg gaaatttttc atgactggtg gaatgcagac caataaccaa attctctaaa
     2941 ttagaacaaa tggttattaa tgaggatttc gataaaaaag aataaaaaaa ccggagccaa
     3001 atgttcattc gactccggtt tattattaga aaagatgatt atgctacgtc ttcgtactgc
     3061 ggaaccggat tacggaagct cttggtgacg caagccaggt aaatcagacc gatagcagcc
     3121 cagatcagac ccagaaccat tgagctttct tccaggttaa cccacagcgc accaacggtc
     3181 agcgcaccac acatcggcag gaacagatac tggaagtgat ctttcagcgt cttgttacgc
     3241 ttctcacgga tccagaactg cgagatgacc gacaggttaa caaaggtgaa cgccaccagc
     3301 gcaccaaagt taatcagcgc cgttgccatt accaggtcga agttgattgc cagcagggca
     3361 atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc atttcgggtg tacataaccg
     3421 aagaagcttt tcgggaacac gccgtcacga cccattacgt acatcagacg cgctacgcct
     3481 gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg agaagatcag cgcgccaacc
     3541 tggaacgctt tacccgcaac gtacagcatg atttcaggct gtgacgcatc cggatcttta
     3601 aagcgagaga tatccgggaa gtacagctgc aggaagtaag ttgcaaagat gaagatcatg
     3661 ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca cgcgctctgc atctttggtt
     3721 tcttccgaca gattgctgat gccgtcaaag ccggtaaagg agaagcacag gattgtcgcc
     3781 ccggtaatca tcgggataac atgcgcatcg ccagaccaga acggacgcgt gctcgccagc
     3841 gtaccagcgc cttcaccttc aaatacgcca taaacaacca tgcccagaat caccgcgatc
     3901 agcactacct gcaacacgac gattacagtg ttgaagttcg ctacggattt cagactacgc
     3961 aggttaaagg cggtcataaa ggccaccagc gccaccacaa acatccacga tggaatggaa
     4021 ggcaccagag cttcaaaata gattttcgcc agcagaatgt tgatcatcgg cgcgaacaga
     4081 tagtcgagca gagaagacca acccaccata aagccgacag tcgggctaat ggatttctgg
     4141 gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa ccagcttccc gtagctcaga
     4201 gccgtaaaca ggatcgcaat caatgcgaac gcataggctg tcggcacatg accatccgtg
     4261 aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg gctgcatata ggctagaccc
     4321 atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt ccacgcgaga ggtgtttgga
     4381 gtaacgttat gcgacatggt tattctcctt tacggtgata accgtcgcgt aagcaaaaaa
     4441 tttgccccat ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta
     4501 tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac
     4561 gctaattaaa cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg
     4621 gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttaaat ggctgatttt
     4681 ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatctcgc
     4741 caccagccgc tggcaagccc tgcggtctgt tcattccagc caatccagac aggttcaaag
     4801 agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgcgcc
     4861 agataccgtg gtaaatagcc gcagccaagg ccgctaattt gcagctccag tttggtttta
     4921 aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct
     4981 gcctgcgcag tgtcccccac cacaatcgcc cgatatcgtt taatgatacg acggtttaac
     5041 ggttcttctt cctgggctaa cggatgatga ggcgcgaccg caaagacttg ttcaagatcg
     5101 cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacgata
     5161 atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac
     5221 ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tgagaggcgc aagcagagaa
     5281 aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg
     5341 atcgcctgct tttccagttc tcgcacggta tgcagaactt cccgcccttt ctctaataac
     5401 atttttccgg tgcgggtgaa tttagcgcgg tgtccgctac gatcaagcaa ttgaatattt
     5461 aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg
     5521 gctgacgccg ccgcaaaact gccctctttt tctatggcat cgaggatcat caacacatcc
     5581 agcagtggtt tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt
     5641 tactccagcg gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa
     5701 atccgactgc catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga
     5761 ggttgcagcc ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct
     5821 ggcgcacata tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg
     5881 ccaataacat cttctaaatg gactaaatta acaccatgtt caccatcagg ggcggttttt
     5941 ccggcaaaga agcgtcccgg atgacgtccc ggtccgacca ggcccgcaag acgcagaata
     6001 tcgaccgaag taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca
     6061 ctgttggtta ccggattacg cggggtagtt tctttaaccg tgccttgcgc gtcgccatag
     6121 acagatgttg agctggtaaa aataattcgg ggaatacgat gggccagcgc gctatccact
     6181 aactcttgta ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc
     6241 gtaatgacca gcgcatcggc atccataagg gcatccagat cgtcagaatc gcaaactaac
     6301 tcaggctcca tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca
     6361 tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc
     6421 ggcataccta accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata
     6481 caccttagat ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga
     6541 atctgttcaa taatgataac gacatgctgc aatgagcagt gaatcatcag taaccgcgta
     6601 taccagacgg tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag
     6661 gggttctggt ttccccttac cttcaaatgg tgttctgcgg gtatctttga taagttcatt
     6721 gatcttttta acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc
     6781 ctcagaccag attagtttca ct
//
LOCUS       DADLTF010000180         6791 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.182, whole genome shotgun sequence.
ACCESSION   DADLTF010000180 DADLTF010000000
VERSION     DADLTF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.182"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            239..2671
                     /locus_tag="JN749_003687"
     CDS             239..2671
                     /locus_tag="JN749_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7369944.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDILCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2702..4198
                     /locus_tag="JN749_003688"
     CDS             2702..4198
                     /locus_tag="JN749_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HAY7369945.1"
                     /translation="MLQKALFFMEYNMSISSVIKSLQDIMRKDAGVDGDAQRLGQLSW
                     LLFLKIFDAQEEALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPAL
                     KNLTAPIDKNPRGYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIY
                     EQILKDLQSAGNAGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKN
                     KYVKSVADHQTLQQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWD
                     EQLDVIVTNPPFGGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDG
                     TLFGEGVKTKIKKLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYE
                     HPYPAGVKNYSKTKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFN
                     LDIKNPHQAETVSHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGK"
     gene            4207..5868
                     /locus_tag="JN749_003689"
     CDS             4207..5868
                     /locus_tag="JN749_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAY7369946.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="JN749_003690"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="JN749_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HAY7369947.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..>6791)
                     /locus_tag="JN749_003691"
     CDS             complement(6477..>6791)
                     /locus_tag="JN749_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369948.1"
                     /translation="FDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRL
                     SLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESA
                     LV"
BASE COUNT         2003 a         1613 c         1549 g         1626 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgt
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcccatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca ttctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcgatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaagtaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga a
//
LOCUS       DADLTF010000181         6781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.183, whole genome shotgun sequence.
ACCESSION   DADLTF010000181 DADLTF010000000
VERSION     DADLTF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.183"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..3524
                     /locus_tag="JN749_003692"
                     /pseudo
     CDS             1..3524
                     /locus_tag="JN749_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3534..6749
                     /locus_tag="JN749_003693"
                     /pseudo
     CDS             3534..6749
                     /locus_tag="JN749_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423056.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1797 a         1780 c         1634 g         1570 t
ORIGIN      
        1 tgtggagcaa gcgaaagata attctttgca accttcccaa acggtaacgg actctctcaa
       61 ggtagcggac ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc
      121 ggctgacaaa aatacagatg ctgttttacc ttggctggcg aaagatattg ctgtcgtttt
      181 tccgcccgaa gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca
      241 gcaagcagac aaattgaccc aattacgtcg cttagcctgt agcgtgtcgc aacgggataa
      301 taaaacagcc acatttgact tcagcacctg ttctttagaa tggcaaaaca ccgtcgccca
      361 agcgatcagt cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt
      421 actcacggca cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca
      481 gatcgtccag gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat
      541 tgatattaaa tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc
      601 tgactacttg gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc
      661 ggaagagtct ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc
      721 agaatggcgg caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga
      781 aattgcccag cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt
      841 ggcgactgat gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt
      901 tgatgattac tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc
      961 agccctgccc cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca
     1021 tatcaatcat ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg
     1081 tcacgatcga atgacgaaag cctgcgctgc gttcccacac gcagcactgg cagaacaggt
     1141 cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc accaactgac
     1201 acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct ccccaccatg
     1261 gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat taggcatcga
     1321 gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat atatctggta
     1381 ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg caaggatggg
     1441 ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag ttgaggcatg
     1501 gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt cacctaccgg
     1561 gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag aaagcagtaa
     1621 aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc atgttacatt
     1681 ttggactcaa aggtttacct ggtttagtta actcaattac acgctatccg caggaagcct
     1741 tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc cgtgccttca
     1801 acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac ccggaacatg
     1861 cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacaa gataacgccc
     1921 gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa gaaatcgccc
     1981 ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg ctcgatccct
     2041 tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca tcgatctgga
     2101 cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc ctccacctcg
     2161 gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg caggtgaaag
     2221 acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc gcctggcaga
     2281 ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt ctcggtaacg
     2341 atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa tcccagcata
     2401 aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc gccctgatgc
     2461 agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg gcaaaagaaa
     2521 agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa gatcggttag
     2581 caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc ccacgccagt
     2641 tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc ggcagccgcc
     2701 tgaaagacct gccaaaacca aacaaaagcg atgatgagtc acaggcgaac gatgctgtta
     2761 accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc cgcccagcag gtagcaaggc
     2821 ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa ctcttcctgg
     2881 ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt tatagcgccg
     2941 acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc accgctgacg
     3001 atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac gttctggaaa
     3061 tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa ctgctaccac
     3121 cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc aacgccagcg
     3181 aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg ctggcaaata
     3241 aaggctggat aaagggcgaa ccacaggatg gaggctggat cggctggatg atcaaacctc
     3301 tcggtcgctg gtcgttaatc atggaaatcg acgaaggttt tgcggtgggt atgtcaccag
     3361 ccgaacttag cgctgagcag ctcttaagca agctgtggct atgggaaggc aaagcggaaa
     3421 gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc gatgccatca
     3481 ccgccagcga gctaattaac gatattgaag ccctgtttga ataaggaaag agcatggaca
     3541 aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa aaaggcaaaa
     3601 caccactcag tcatcgcaat tggccgggcg aaccagtgcc tgttatcact gaaagcatca
     3661 ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac attgtctggc
     3721 gttatgaaaa tttttcaccg gagtggcagt ccgccattac gcaggccatc aacttgatcg
     3781 gcgaacacaa accctcagtc ccggcccgca caatggcggc actagccttt atcgcgcaaa
     3841 atgacaacca atagttgctc gacgaaatcg tccaacaaga ggggctggaa tatgcgactg
     3901 aggtggtgat tgcacgccag tttattgcac ggtgttatga gagtgatcct ctggtagtca
     3961 cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc tataacgaat
     4021 tcgatctccg actgcgtaaa cacctctctc tggcagagga aagctgctgg cagcgttgcg
     4081 ccgacaaact cattgccgca ctgccaggaa taaccaaagt tcgccgccct tttattgcgc
     4141 tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa tgcccgcgga
     4201 ctcattttca ttctaaggag tggttaaaag ttgttgctac tgaccccaaa gcggtgagaa
     4261 aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc tacatgtcgc
     4321 atgaaaacca cttcggctac gcggcctgcg ctgccctttt gcgcgaacaa ggactggcag
     4381 tcgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg ctggtacaaa
     4441 ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac aaacccagcc
     4501 tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc gcactggcag
     4561 aactgctggc gttaaaagaa ccaccagccc gcccaagtta tccaatcatc gaagacaaaa
     4621 agctgcctgc acagcaaaaa gctcgagatg aatactggcg tacgctgtta caaacgctga
     4681 tggcatcgca gccacaactg gcagcagaag tgatgccgtg gttaagtact caagcccggg
     4741 cagtgctgaa tagttattta tcggcaccgc ccaaaccagt tattgatagc accgataaca
     4801 gccaaatgcc tgaaatcctc gtttcaccac catggcgtgg gagaaagaaa aagacttcca
     4861 tacgtcactt ttcgctgcct gatcttacgc ttgcaccgca aacgtacagg tcgccgaata
     4921 aacactggac gccggaacag caaactgcaa ttgaccattt tagcgccatg tcttttgacg
     4981 aacgtcttgc tacgagagga gcggatatct tcttgcggga attaggtttt gacgttcata
     5041 gcctggaatt taataagcgt cttcttaatg gacagttgga tgcctttgat gctcagcaac
     5101 tgcaagaata cacatcaata ggactattgc gtacttatca cgcgcatcag gccaaatcta
     5161 aacagtaccg ggaagacgcc accaatgcat tactggcaca agatagcatt gcgttgatgg
     5221 gagggtggcg aacttttcag cacggatttt actatggtaa tgaacagcgt tgggggattt
     5281 ggaatctgta ccttatcgcg cagatgccgc gcgaaatggc ggtatcgtgc tggcaacaaa
     5341 ttgtcgaggc tgattttcat tacaccgggg tggaatattt gttaagtgtg ctgggtaccg
     5401 atgctttgcc tggattaaac accgcctttg cccgacatcc aaaagagata tttccgctgc
     5461 tgattcattt cggcgcaaca gaactggccc tgcccattgc ccgcgtctgg cacctttttg
     5521 cgggccagcg taatctggct cgccagtgga ttttacaatg gccggaacat acggctactg
     5581 cacttatccc tcttgtcttc accaaatcta gcgataaaag cgaagctgca ttacttgccc
     5641 tgcgtttact ttacgaacat gggcatggcg aattgctaca aactgtagcc aatcgttggc
     5701 agcgtacaga tctatggcct gccctggaac aattacttaa acagagtcca atagagattt
     5761 acccggcacg cattccaaaa gcccctgatt tctggcatcc gcaaatgtgg tccaggccgc
     5821 gccttatcac taataatcaa cctgttaccg atgacgctct ggaaattatc ggcgaaatgc
     5881 tgcgctttac ccagggggga cgtttttata gcgggctgga acaactgaaa acgttctgcc
     5941 agccacaaac gctggcagct tttgcctggg atctcttcac tgcgtggcaa caagctggtg
     6001 cccccgcaaa agacaactgg gcatttctgg cgttaagtct ctttggtgac gaaagcacgg
     6061 cacgggatct aacgacacat atcctcgcct ggccacaaga aggcaaatct gcccgtgcgg
     6121 tcagtggcct gaacatcctt accctgatga ataatgatat ggcgctgatg cagctgcatc
     6181 atatatcgca acgggcgaaa tcccgcccct tacgtgataa cgcggcggaa tttcttcagg
     6241 tagtcgcaga aaatcgcggg ctaagccagg aagagttagc ggacagatta gtcccaacac
     6301 tgggccttaa tgatccgcag gcgttgagtt ttgattttgg tccccggcag tttaccgttc
     6361 gcttcgatga aaacctcaac ccggttatct ttgatcagca aaacgttcgc cagaaaagtg
     6421 ttccccggtt gcgcgccgat gacgatcaac tgaaagcgcc cgaggcactg gcccgactaa
     6481 aagggttaaa aaaagatgct actcaggtga gcaaaaacct gctcccgcgt cttgaagctg
     6541 ccctacgtac cacccgacgc tggtcgctgg cagattttca ttctctgttt gttaatcatc
     6601 cctttacccg tctggttacc cagcgattaa tatggggggt tatccggcaa atgaaccgcg
     6661 tcgtttactc aacgcctttc gtgtggccgc agagggggag ttctgcaatg cgcaagatga
     6721 gccaattgac ctgcctgcgg acgctctgat tggcattgcc cacccgttag aaatgacagc
     6781 a
//
LOCUS       DADLTF010000182         6715 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.184, whole genome shotgun sequence.
ACCESSION   DADLTF010000182 DADLTF010000000
VERSION     DADLTF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.184"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..766
                     /locus_tag="JN749_003694"
     CDS             <1..766
                     /locus_tag="JN749_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7369949.1"
                     /translation="NDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            777..1850
                     /locus_tag="JN749_003695"
     CDS             777..1850
                     /locus_tag="JN749_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7369950.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSSLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2098..3138
                     /gene="pstS"
                     /locus_tag="JN749_003696"
     CDS             2098..3138
                     /gene="pstS"
                     /locus_tag="JN749_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAY7369951.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLEKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3225..4184
                     /gene="pstC"
                     /locus_tag="JN749_003697"
     CDS             3225..4184
                     /gene="pstC"
                     /locus_tag="JN749_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HAY7369952.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4184..5074
                     /gene="pstA"
                     /locus_tag="JN749_003698"
     CDS             4184..5074
                     /gene="pstA"
                     /locus_tag="JN749_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAY7369953.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5166..5939
                     /gene="pstB"
                     /locus_tag="JN749_003699"
     CDS             5166..5939
                     /gene="pstB"
                     /locus_tag="JN749_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HAY7369954.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPELRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            5954..6679
                     /gene="phoU"
                     /locus_tag="JN749_003700"
     CDS             5954..6679
                     /gene="phoU"
                     /locus_tag="JN749_003700"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAY7369955.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
BASE COUNT         1697 a         1572 c         1746 g         1700 t
ORIGIN      
        1 aaacgataat tccggtagct cacatcaact cggactcagc ggtacggcgc tggaaagaaa
       61 taacctttca tggaatttaa tgcagtccta taacagtcat gatgatgagg ttggcggtaa
      121 tatgtccctg acctatgatg gctcttatgg cacggtgaac ggcagctata actacagcca
      181 aaattcccag aggctgaatt atggtatcag agggggaatt ctggcacaca gcgaaggggt
      241 aacgttaagt caggagttag gtgaaactat tgctcttgtt aaagcacctg gggccgccgg
      301 gttagaaata gataatatgc gcggtgctgc gacggactgg cgcggctata cggtcaagac
      361 acagctaaac ccttatgatg aaaatcgggt agcaatcagc gataactatt tctcgaagtc
      421 gaatatagaa cttgataata ccgtcgttac gatggttccc acgcgtggtg cagtggttaa
      481 agcggagttt gtgactcatg tgggttatcg cgttctcttc agagtgttaa atgcaaatgg
      541 taaaccggta ccttttggag ccattgctgc gatacaagat gcaagtttgg cagattcagg
      601 aattgtcggt gaccgtggcg aactttatct ttctggtcta ccagaaaaag gacaggttac
      661 gttatcctgg ggagaaaacg cctcaacaaa atgcatcttc aattattcac tttcgacacc
      721 agaaagtgag agcggattaa ttgaacaggg tgtgacatgt cattaaaagg atttttatga
      781 acaagtatat caaacagtgg tgcttttctg tgtttatgct ctcgttaagt agtgtagcac
      841 ttgcagctcc taaaggtatc tgtacccctg ataatggcgt atttcacagt tcgcttgatt
      901 tctcgggata tcttattacc gcgaacgaaa ataaggttgg aacgacattt aacaccactg
      961 taactaatgg atcctcatat cttggacgct gtcattgtga tactggtaac gtaggagaat
     1021 tcccctatat ctattatacg tcaaaaataa accaggcatt aacttatgct ggggttcact
     1081 ctaatattaa ttattatgat cttaatccga accttgatgt tgggattgct atagatattc
     1141 ttggggttgg gtatgtgaat gcaccttttg aatatcatgc taacaacccg tctggtaata
     1201 caaagtacaa ttgcaaccgc attgaacctt taagtatatc cagtggggct aaagcgatag
     1261 tatattttta tatcaagaag acatttgcag gaaaattgat tattcctgaa acaaagatag
     1321 tgacattgta tggaacaatt agccgtgaca ccccggtgga ttactcacag ccgatggctg
     1381 atgtttatat tcggggcgat attacagctc cgcaaagttg cgaaataaat aatttaaagc
     1441 cagtttgttt tgattttaaa gaaataccag ctgcagattt ttcatctgtt gttggaagtg
     1501 cggtaacaac acataaaatt actaaaacag tcactattga gtgtgaaaac ctagggatac
     1561 tgaataccga tgatatcagt acgtcttttt atgctaccga acccaatacg gacaattcaa
     1621 tggtcgtaac ttcaaactct aacgtgggga taaaaattta cgacaaaaat aataaggaaa
     1681 tcaaagtgaa cggtggtgag ttgccaacag acatggataa atcaacagtg tatggtgaaa
     1741 aatcaggtag tgtaactttt tcagctgctc ctgcgagcct tactggagct cgcccagccc
     1801 cggggcaatt tactgcaaca gcgacgataa ctgttgagat tgtgcgctaa taggtctgac
     1861 aacgattgtt catcacatcg ttgtcatctc acccatttct ttatcacttt taaatgcatc
     1921 atgacaaaaa gatggcatgt gggattttca tcttcacatt taaaatcagt aagttatttg
     1981 gtaattcctt tctgtcataa aaatgtcata ttccttatat ataactgtca cctgtttgtc
     2041 ctattttgct tctcgtagcc aacaaacaat gctttatgaa tcctcccagg agacattatg
     2101 aaagttatgc gtaccaccgt cgcaactgtt gtcgccgcga ccttatcgat gagtgctttc
     2161 tctgtgtttg cagaagcaag cctgacaggt gcaggtgcaa ccttccctgc gccggtgtat
     2221 gccaaatggg ctgacactta ccagaaagaa accggtaata aagttaacta ccagggtatc
     2281 ggttcttccg gtggcgtaaa acagattatc gctaataccg ttgattttgg tgcctctgac
     2341 gcgccgctgt ctgacgaaaa actggctcag gaaggtctgt tccagttccc gaccgtgatt
     2401 ggcggcgtgg tgctggcggt taacattcca gggctgaagt ctggcgaact ggtgctggat
     2461 ggtaaaaccc tcggcgacat ctacctgggc aaaatcaaga agtgggatga tgaagccatc
     2521 gccaaactga atccgggtct gaaactgcct tcacaaaaca ttgctgtagt acgccgcgca
     2581 gatggctccg ggacttcctt cgtcttcacc agctacctgg agaaagtgaa cgaagagtgg
     2641 aaaaacaacg ttggtactgg ctctaccgta aaatggccga tcggtctggg cggtaaaggt
     2701 aacgacggta tcgccgcgtt cgttcagcgt ctgccgggtg caattggtta tgttgaatat
     2761 gcttacgcga agcagaacaa cctggcgtac accaaactga tctccgctga tggtaaaccg
     2821 gttagtccga ccgaagaaaa cttcgctaat gcagcaaaag gtgcagactg gagcaaaacc
     2881 ttcgctcagg atctgaccaa ccagaaaggc gaagatgcat ggcctattac ctctaccacg
     2941 ttcattctga tccacaaaga tcagaagaaa ccagaacaag gcacagaggt gctgaaattc
     3001 ttcgactggg cgtacaaaac cggggctaaa caggcgaacg acctggatta cgctagcctg
     3061 ccggatagtg tagttgaaca ggttcgcgct gcgtggaaga ccaatattaa agacagtagc
     3121 ggtaagccgc tgtactaata aaactccagg ccgggtacgg tgttttacgc cgcatccggc
     3181 attacaaaat gactttgtaa acgcgtttaa ctgaagagta acttatggct gcaaccaagc
     3241 ccgcttttaa cccaccgggt aaaaagggcg acataatttt cagcgtgctg gtaaaactgg
     3301 cggcgctgat tgtgctattg atgttgggtg gcattattgt ctctctgatc atctcctcct
     3361 ggccgagcat tcagaaattt ggtctggctt tcctatggac caaagagtgg gatgcaccga
     3421 acgatatcta cggggcgctg gtgccgatct acggtacgtt ggtgacttcg tttatcgcgc
     3481 tgctgatcgc cgtcccggtg agtttcggta tcgccctgtt cctgactgag cttgcgcctg
     3541 gctggctgaa acgcccgctg ggtatcgcca ttgagctgct ggcagccatt ccaagtatcg
     3601 tttacggcat gtggggcctg tttatctttg cgccgctgtt cgccgtttac tttcaggagc
     3661 cggtcggcaa tatcatgtcg aatatcccga ttgttggcgc gctgttctct ggccccgcat
     3721 ttggtatcgg tatcctcgcg gcaggcgtga tcctcgccat catgattatt ccgtacattg
     3781 cggcggtaat gcgtgatgtg ttcgaacaaa ccccggtgat gatgaaagag tcggcctacg
     3841 gtattggctg caccacctgg gaagttatct ggcgtatcgt tcttccgttc accaaaaatg
     3901 gtgttatcgg cggcatcatg ctggggctgg gccgcgcgct cggtgaaacc atggcggtga
     3961 cctttatcat cggtaacacc taccagctcg acagcgcctc gctgtacatg ccgggcaata
     4021 gtatcacctc tgcgctggcg aacgaatttg cggaagcgga atccggtctg cacgttgccg
     4081 cactgatgga actgggcctg atcctgtttg tgattacctt catcgtcctc gccgcatcga
     4141 agtttatgat tatgcgcctg gctaagaatg agggggcacg ctaatggcta tggttgaaat
     4201 gcaaaccact gcggcgctgg ctgaatctcg ccgcaaaatg caggcgcgtc gccgcctcaa
     4261 aaaccgtatt gcgctgacgc tctcgatggc gacgatggcc ttcggcctgt tctggctgat
     4321 ctggatttta atgtccacca tcactcgcgg tatcgacggt atgtcgctgg cgctgttcac
     4381 tgaaatgacg ccgccgccca atacggaagg tggtggtctg gcgaacgccc tggcgggtag
     4441 cgggctgtta attttgtggg ccacggtatt cggtacgccg ctgggcatta tggcggggat
     4501 ttatctggcg gaatatggtc gaaaatcctg gctggcagaa gtgattcgct tcattaacga
     4561 cattctgctc tccgcgccgt cgattgtggt tggtctgttt gtctacacca tcgtggtggc
     4621 acagatggag cacttctccg gctgggcggg cgtaattgcg ctggcgctgt tgcaggtgcc
     4681 gatcgttatc cgcaccaccg agaacatgct gaaactggtg ccggacagcc tgcgtgaagc
     4741 ggcttatgcg ctgggtacac cgaagtggaa gatgatctcc gcgattacgc tgaaagcgtc
     4801 ggtgtccggg attatgaccg gtatcctgct ggcgattgcc cgtattgccg gtgaaaccgc
     4861 gccgctgctg tttaccgcgc tctccaacca gttctggagc acggacatga tgcagccgat
     4921 cgccaacctg ccggtgacga tctttaagtt tgcgatgagc ccgtttgcgg aatggcaaca
     4981 actggcctgg gccggggtat tgatcattac cctgtgcgtc ctgctgctga acattctggc
     5041 gcgcgttgtt tttgcgaaga ataaacacgg ttaacacttt gccggatgcg gcgtgaacgc
     5101 cttacccggc cctacggtaa gcctgattag cgaaaagcat caggtacgat gaggaaaaga
     5161 ttgcaatgag tatggttgaa actgccccga gtaaaattca ggttcgtaat ttgaacttct
     5221 actacggcaa attccatgcc ctgaaaaaca tcaacctgga tatcgccaaa aaccaggtta
     5281 cggcgtttat cgggccgtcc ggctgcggta aatcgacgct gctgcgtacc ttcaacaaaa
     5341 tgtttgaact gtacccggag ctgcgtgcgg aaggtgaaat tctgcttgat ggcgacaaca
     5401 tcctgaccaa ctctcaggat atcgccctgc tacgtgcgaa agtgggcatg gtgttccaga
     5461 aaccgacgcc gttcccgatg tccatctacg acaacatcgc ctttggcgtt cgtctgtttg
     5521 agaagctctc ccgtgccgac atggacgagc gcgtgcagtg ggcattgacc aaagccgcat
     5581 tgtggaacga aaccaaagat aaattgcacc agagcggtta ctctctctct ggtggtcagc
     5641 aacagcgtct gtgtattgcg cgtggtatcg ccattcgccc ggaagtgctg ctgctcgacg
     5701 aaccgtgttc ggcgctcgac cccatctcta ccgggcgtat tgaagagctg atcaccgaac
     5761 tgaagcagga ttacaccgtg gtgatcgtta cccacaacat gcagcaggct gcgcgttgtt
     5821 ccgaccacac ggcatttatg tacctgggcg aattgattga gttcagcaac acggacgatc
     5881 tgttcaccaa gccagcgaag aaacaaacag aagactacat caccggtcgt tacggttgat
     5941 tcaggagtgc gttatggaca gtctcaatct taataaacat atttccggcc agttcaacgc
     6001 cgaactggaa agtatccgca cgcaggtgat gaccatgggc ggcatggtgg agcagcagct
     6061 ttctgatgca atcaccgcga tgcataacca ggacagcgat ctggcgaagc gcgtgatcga
     6121 aggcgacaag aacgtcaaca tgatggaagt ggcgatcgat gaagcctgcg tgcgcattat
     6181 cgccaaacgt cagccgacgg cgagcgacct gcgactggtt atggtgatca gtaaaaccat
     6241 tgccgagctg gagcgtattg gcgacgtggc ggacaaaatc tgccgtactg cgctggagaa
     6301 attctcccag cagcatcagc cgttgctggt aagtctggag tcgctgggcc gtcataccat
     6361 ccagatgctg cacgacgtgc tggacgcgtt cgcgcggatg gacattgacg aagcggtacg
     6421 tatttatcgt gaagataaaa aagtcgatca ggaatacgaa ggtattgttc gtcaactgat
     6481 gacctacatg atggaagatt cgcgcactat tccgagcgta cttaccgcgc tgttctgtgc
     6541 gcgttcaatc gaacgtatcg gcgaccgctg ccagaatatt tgtgagttta tcttctacta
     6601 cgtgaagggt caggatttcc gtcatgtagg cagtgatgag ctggataaac tgctggcggg
     6661 aaaagatagc gacaaataat tcaccagaca aatcccaata acttaattat tggga
//
LOCUS       DADLTF010000183         6707 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.185, whole genome shotgun sequence.
ACCESSION   DADLTF010000183 DADLTF010000000
VERSION     DADLTF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.185"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            637..2220
                     /locus_tag="JN749_003701"
     CDS             637..2220
                     /locus_tag="JN749_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HAY7369956.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2672..4525)
                     /gene="asmA"
                     /locus_tag="JN749_003702"
     CDS             complement(2672..4525)
                     /gene="asmA"
                     /locus_tag="JN749_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HAY7369957.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4547..5128)
                     /gene="dcd"
                     /locus_tag="JN749_003703"
     CDS             complement(4547..5128)
                     /gene="dcd"
                     /locus_tag="JN749_003703"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HAY7369958.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(5220..5861)
                     /gene="udk"
                     /locus_tag="JN749_003704"
     CDS             complement(5220..5861)
                     /gene="udk"
                     /locus_tag="JN749_003704"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAY7369959.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            6179..6601
                     /locus_tag="JN749_003705"
                     /pseudo
     CDS             6179..6601
                     /locus_tag="JN749_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
BASE COUNT         1573 a         1746 c         1688 g         1700 t
ORIGIN      
        1 agtaattatc ctgtgcacta ttaatagcaa tgtcgccatg cacatttacc ttgcagttaa
       61 ttgaataaaa atttaactgg catcagtcct aaaaaaattg atttcatccg caggctattg
      121 acagaataat tcagactggt ctttcaggca tccagacacg ctaccgcccc tggcttttta
      181 gctaccaata cactgattga gcttaatttt tcaaaccctc tctgcataca atcgttgatg
      241 agaaagggtt attgcggaaa ttaacttccg aatataaggt gacattatgg taattgaata
      301 ttggctcgcc aataatgcag gaggaagtgt tacagctaac ggaatagcag gcaaggtaac
      361 aattcggcaa ttggctattt ttaagaatta tattaagtgt cattcaatat ggtttttagg
      421 agtttcttta ggttgacaat atttaatatt gcgtctccac atgcgatatt tcttaaataa
      481 tgttttatta ttaccgcttt taattcaggg ataatgtgag gttattaccc tcaaataata
      541 tgagataaat agtgctgcaa cattgcattt ttgccccgat ttatccatga tcgaattgtg
      601 acatttgtca tacaacgaat aggttttgta cttactatgg aatggattgc cgatccgtct
      661 atctgggccg ggttaatcac gctgattgtg atcgaactgg tcctcggcat tgataacctg
      721 gtctttattg ccatcctcgc cgaaaaacta ccgccgaagc agcgcgaccg cgcacgggtt
      781 accgggctgc tgctggcgat gttaatgcgc ctgttactgc tagcgtcaat ctcctggctg
      841 gtcaccctga ctcaaccgct gttcagcttc cgctcgttta cctttagcgc ccgcgactta
      901 atcatgctgt ttggtggttt cttcctgttg ttcaaagcca cgatggagct gaacgaacgg
      961 ctggaaggga aagacagcaa taatcccaca caacgcaaag gtgcgaagtt ctggggcgtg
     1021 gtgacgcaaa ttgtggtact ggacgccatt ttctcactcg actcagtgat taccgccgtc
     1081 gggatggtcg accatttact ggtcatgatg gccgccgtgg ttatcgctat cagcctgatg
     1141 ttgatggcca gcaagccgtt aacgcaattt gttaacagtc acccgaccat cgttattctc
     1201 tgcttaagct tcctgttgat gattggcttt agcctggtgg cagaaggttt cggcttcgtc
     1261 attccgaaag gctacctgta cgctgccatt ggtttctcgg tgatgatcga ggcactcaat
     1321 cagttggcta tctttaaccg gcgacgtttt ctttccgcta atcagacgct acgccagcgg
     1381 actaccgaag cggtaatgcg cctgcttagc gggcaaaaag aagatgcgga actggacgcc
     1441 gaaaccgcgt ccatgctggt ggatcatgga aaccagcaga tctttaatcc gcaggaacgg
     1501 cggatgattg agcgggtact taatcttaac cagcgtaccg tcagcagcat tatgacgtcg
     1561 cgccacgata ttgagcatat cgatctcaac gcgccggaag aagaaatccg ccagttgctg
     1621 gagcgaaatc agcatacgcg gctggtggtt accgacggtg atgacgcaga agatttgctc
     1681 ggtgttgttc acgttatcga cctgctacaa cagtcactgc gcggcgaacc gctcaacctg
     1741 cgggtgttga ttcgccagcc gctggtgttc ccggaaacct tgccgttgtt accagccctg
     1801 gagcagttcc gtaatgcccg cacgcacttt gcttttgtgg tggatgagtt tggctcggtg
     1861 gaagggattg tgacattaag tgacgtcact gaaaccattg ccggtaactt accgaacgaa
     1921 gtggaagaga tcgacgcccg ccatgatatt cagaagaatg cggacggttc ctggacggcg
     1981 aatggtcata tgccgctgga ggatctggtg caatatgtgc cgctgccgct ggatgaaaaa
     2041 cgtgaatatc acaccattgc cgggctgttg atggagtatt tgcagcgtat tccaaaacct
     2101 ggcgaagaag ttcaggtggg ggattatttg cttaaaacgt tgcaggtgga aagccatcgc
     2161 gtgcagaagg tgcagattat accgctgcgt aaggatggcg agatggagta cgaggtgtga
     2221 tggtatcggc cattttaccc aatgctgcgt aacgccttat aacttcagca atgaagcgtt
     2281 gtctgtggta aacggacatc acagcggggg tgtgggtccg gctgtaaatc gccgaatcgt
     2341 ttcctgtagg ctggggcagc ccacatggat gtgggctgag ggcgcgtttt acagggatgt
     2401 tacctcgcgc ccgtacccga tagccgaaag gaataagatg agggaaccgc gccagcggcg
     2461 atttaccgcc gggagcccgg gttgccaggg tggtggcgat tgagccaccc tggcacgttc
     2521 accggcttga gcgttccata gtagcgagga acatcaagtg aacggaacag ccttcgatct
     2581 tacatattca gaatataata gtgccgccgc gaacgcctga tgcgacgctt tacgcgtctt
     2641 atcaggccta caggacaggt cacctggtaa cctacatctt ctccagcaac ttcttcacat
     2701 ctttgccatt gcgggaatct ttattccgct cggcccagtc attcaggcga cgtttcgctt
     2761 cgtcctgtag atgtttgcgc agtaactgat ccacttgcag gctgtaattg agttgctgcc
     2821 agttgccata aacccgcagc ggtactggcg tttctttgag gaaatcaatc agtttgcttt
     2881 ccccgttcca gccacccacg acccgaatat caaactgggt gtcgcaggtt tgatctgcca
     2941 gattcaacat gccttcccct gtcagcgcca gcacaggcga ttgaccttgc atgtcgttta
     3001 acgtcacgac gccatccttc aacgtcaaat cggtggtaaa gcggtcaagc cgcgtcacgt
     3061 tatcgaagtt ttcagcggcc ttcacatcac cgccattacg ctctaccgct tgctgaatca
     3121 tctgctggaa gttcatccct tccatacgtg tgtcggtcat ctcaacatgc gcctgccctt
     3181 gccagttgtg gcggaatgcg tcggcatcta tgtcagcacc ggagaaatca ccagccagtg
     3241 acatttttcc ggtcaacgaa atcggatagt taaacgcctt cagaatggta ccaatctcaa
     3301 cgttctccag ccgtggctgg aagtttatcc gcggatttat tgatgtcgcg tccagcgtgc
     3361 ccggcagtga aacctttcca ccgttaagct tgccctgcag ttgagtaatt tccagcaaac
     3421 ccgacttgtt ggtcatttgc gtggcaacat ctgtaaaatt cattccgcgc cagcgcacgt
     3481 tactggcctg caacaaaata tcagctgtaa agccttgcag tccctgatag gccggttcat
     3541 caatacgcga agaaatgacc gggcgcggca acgttgattg gctctgcccc tgctgcgcgg
     3601 caccgttttc accattcgcg gtttcattaa gcgggatgag gttgtcaaga ttcagttgcg
     3661 ggaattgcag tctcagctgc cattccggtt tctctgtcag cgtgacctgt gcttgcccgc
     3721 tcagcgtact gtcattggcg gtcaaactaa tttggttaaa tgaaaggcgt ttatgtgact
     3781 cctgccactg ggactggaaa ctcccctgtc cctgaatacc ttgttttggt aaatcggcac
     3841 cctgcaactg ccagttaatt tgttcaatag ccgctgttaa atcatgcgga taatcagaag
     3901 catctaccgt accgttaagg gatatcgtga gatcgcgctg atcgcgatta acccgcccgg
     3961 agaactcaaa tgagccacga tgttggggat cttgttccat ttgcaggcgg atattgcgga
     4021 ttgtcacctg ctcgtcatct tcatgctgga acaccagaac gctgtccgcc accttaagac
     4081 tggatatatc aaacgaccat ccgcgatcgt ctgacagatc cggcaaggta ttgtcgcgcg
     4141 gtgcaaccgg agcgtcttca ctgcgcaccg cttccgtctg cggcgtcagt tggatcactg
     4201 ccccttttag catcacctgc ttaacgctca gttgatgact cagtagtggt aaaagcgcca
     4261 cgtccagacg catgttgtcg gcgcgaacca gtggctggct tgcgccctgg gcggtgagag
     4321 acattcgccc ggagaggata ctaagctgcg gccagacgtg ccaacgcagt ggcccgtcga
     4381 gctgcaattg ataaccgcta cgtgcagcaa cttgcttgac catatagtcg cggaaatcat
     4441 tcggattcac cagcaacact aacgcagata acccggccac cagcacgacc aggagtatca
     4501 tcagcgtcgt cagaaatcgt ctcatggtat cctcaatggg cctgaattag tctttatcga
     4561 ttcggctggc taccgcgccc tgctggttgc gatatttcgc atcttcacgg cggttgtaag
     4621 gtcgcgccgc cgggccggaa agcggctcaa agctcagcgc accaattaac atgcccggac
     4681 gcagcgccag cggcagctta ccggagttgt agaactccag cacaatgcaa ccagaccagc
     4741 ccggatcgat acggtgcgcg gtgacgtgca ccatcagccc cagacgcgcc aaagaggaac
     4801 gcccgtccag ccagcccacc agatcggctg gcagcgtcac cgactccagc gtcaccgcca
     4861 gcgccagctc tcctgggtga agatagaacg cctcgccctc gtcgagaacg atctcatcgc
     4921 tcatcacgcg gtcaagcgcg gcgctcactt catctttggg accgctcaga tcgataaacg
     4981 ctgccgtgtg accacggaag gtacgaaatt tattgcccag gcgtacatcc accgtcgcgc
     5041 cgttaatacg ctccactggc ggacgtgggt tgatcgacaa acggccttca tcaagccagg
     5101 cttcaatatc tcggtcacac agacgcatgg cattttctcc tttcgcgcat cactccctta
     5161 acgccaatta cgtcaagggc ataccagctt atcactgaac ggtacacaat ttatcaagct
     5221 tattcaaaga actgacttat tttcgctttc aatatatcga tcgcgatgcg gtttttcccg
     5281 ccgcgcggca cgataatgtc cgcatattgt ttagaaggct caatgaattg caggaacatc
     5341 gggcgcacgg ttttttgata ttgcgccatc acggaatcca ttgaacgccc acgctcgtta
     5401 acgtcacgct tgatgcggcg catcaggcag atatccagcg gggtatcaac gaaaatggag
     5461 aagttaagtt cgtcacgcaa acgcgcatcc gtcagcaaca aaatgccttc gagaatgatg
     5521 accttcttcg gctcaacctt caccgtttct ttcatacgcg tatgttcaac atagctgtaa
     5581 accggcaggt caattgccga gccgcgtttc aacgcttgta aatgctcaag caacaggctg
     5641 tgatccatcg cgctgggatg gtcgtagttg gtcttaacgc gttcttccat cgacagatgg
     5701 ctttgatctt tgtaatagca gtcttcggga attacgccga tgtgttcatc accgacttgc
     5761 tcacgtaatt cacgataaag ggtactggca ataagactct tgccggaagc cgatgcgcca
     5821 gcgataccga taatgacgca ctgatgagac tgatcagtca tatatttagc gacctgatta
     5881 acctggatgt taggaagggg gcgacgaagc gccaaacgcg gcaattatag ggatttcatc
     5941 cgtctgatac cagtcgaata gcgttgccgc gcgctcagag ttaattgttg acaaagaatt
     6001 cccggtggca aattacgttg atcagtttta tgtaaggtaa aaaatgttat acgcagttgc
     6061 gcaaattatc cgcctttacg tcactttatg agcaattcgc atataaaatg taaaactttt
     6121 gtactagcat aaacacagaa acgaatactg gcgacctggc cttgcggata aagcggtaat
     6181 aagcaaacaa tcacagcatg tattaattgc cctgccccac ccgctgcttc acctggtcag
     6241 tttaggttta gtctcgttta tctttactct tgtctcgctt gagctttcgc agtttggcac
     6301 ccaactcgcc ccactgtggt tcccgacgtc catcatgatg gtggcgtttt atcgccatgc
     6361 cgggcgcatg tggccgggaa ttgcgctgag ctgttcgctg ggaaatatcg ccgcatccat
     6421 cctgcttttt tccaccagct cgctgaacat gacctggacg accatcaata ttgttgaagc
     6481 cgtggtcggg gcagtgctgc tacgtaaatt gctgccgtgg tataacccct tgcaaaatct
     6541 ggctgactgg ctgcgtctgg cattcggcag cgccattgtt ccgcctctgt tggggggtgc
     6601 ccctattaag tagtcaagca tgttatgacc cctgcggggt ataaaaagtc ccgtcgcgca
     6661 tcatggcgaa cagaacgtcg cagcgtcgtc tcgccagggc gataagc
//
LOCUS       DADLTF010000184         6694 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.186, whole genome shotgun sequence.
ACCESSION   DADLTF010000184 DADLTF010000000
VERSION     DADLTF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.186"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            358..579
                     /locus_tag="JN749_003706"
     CDS             358..579
                     /locus_tag="JN749_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HAY7369960.1"
                     /translation="MLSQRGGLEIQSQFLRGNEQVYQCQVAFVLPGERVMMAFTYART
                     TPLTPADMTRWAEIKKNLRFRMRQEVRTN"
     gene            598..5262
                     /locus_tag="JN749_003707"
     CDS             598..5262
                     /locus_tag="JN749_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HAY7369961.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGGTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNTWRCVYDERGNLLAVTDPLQQNTRYQYDHHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAQSHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDAMGRRTAF
                     GYDAYGRLVTLTNENGERYWFRHDVLDRLAEQINPDGCRQSYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETADGRTEYVHDAADQLLEIRRQRHETDAPEVIRFSYD
                     RLGRMLSEETAQGILTHQYDELGNRTATTFPDGRTQRHLYYGSGHLQQINLDREVISE
                     FTRDHLHREVLRSQGRLSTRQLYDPGGRLKRRETYSGMRGVVPETFTDRQYSYSGEDE
                     LLKTRHSRRGETDYFYDPTGSITACRSEDEGYLASWQYDAAGNLLGRRAGERATAENS
                     VVPFNRLMSYRGVHYRYDEFGRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYD
                     ALGRRTEKQHISPDGKPYNRTKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVD
                     KAGKEGPNRILYFHTDVNGAPEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRY
                     QGQYLDRETGLHYNLHRYYDPDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTP
                     CAVSNQKANRLLDSSETKVTVRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYL
                     TGNKTKAGSYHWDDIKDPSVTKPPYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPR
                     ET"
     gene            5265..5645
                     /locus_tag="JN749_003708"
     CDS             5265..5645
                     /locus_tag="JN749_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369962.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     EEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYTL"
     gene            complement(5700..6017)
                     /locus_tag="JN749_003709"
     CDS             complement(5700..6017)
                     /locus_tag="JN749_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HAY7369963.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            6146..6655
                     /locus_tag="JN749_003710"
     CDS             6146..6655
                     /locus_tag="JN749_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HAY7369964.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT         1639 a         1631 c         1994 g         1430 t
ORIGIN      
        1 tgttgttaat aaaaagctta tagatttata gttgataatt aaaacgcatc cgatatttca
       61 gctttttcta tgcaagatac aattttataa ggtgtccatt tttatatgga ggcggtagcg
      121 tgtctgttac tagtggtttt atttgcatag tgaatatatt gggattgtta aatacataac
      181 tatgtatccg ctctggtcgt tgtaaatatt gctaattgct taatattatt agtgattatg
      241 gctggaaaga taaagtgtgc tgtaaagcgg tgatgggtat tacattcatg attaaaaaaa
      301 cagaacctgt caataaataa ttcctttatt aaaacggact atttacatta tgacggaata
      361 ttatctcaac gaggcggttt ggaaatacag agccagtttc tccggggtaa cgagcaggtg
      421 taccagtgtc aggtggcatt tgtattgccg ggtgagcggg tcatgatggc cttcacctac
      481 gccagaacca caccgctgac cccggcagat atgacgcgct gggctgaaat taaaaagaat
      541 ctgcgcttca ggatgcggca ggaagtcagg acgaattaat catacaggga acgggacatg
      601 gaagaggcat tctgggcggc aagacagggg gatgccctgc tgcatacgtc gtttatggca
      661 gatgtgctgg gggcggtgct ggaggtggct gcaaatgtcg ttattgatgc cctcattgtc
      721 ggcacaacct cgttactggc gacgtcagtg gggatcacga tggggggcac ggccgtcgtg
      781 ctgaccggtt ttgtggcggg tgtggcgatg gtgtacaccg gcgtgacaga aaaggtcagc
      841 gaagcctgtt cagccctcgc aaatatgctt ttcccgcctc agattgaagg ttacatcctc
      901 accggctctg gtgacacatg gatcaacagc aaacctgccg cgcgggcagc ggcgactgcg
      961 gcatcgcggc aggacataga ggcgcaggaa gcgcaggcga aggcagaaca ggaagaagcg
     1021 cagcgtcagg aggatgccag aacgttccgg gatgtggccg gggaatatct ggaaatggcg
     1081 ggggccatag ccctggcggt taacccggtg acagggcctg tgctgatgtc ccgggctctg
     1141 cacgaccagc tcagtacgga agaagggcgt tctgaaatgg tggatggcgt gacacatttt
     1201 gtgtcggaac tatggcagcc cactgtggcc agtgcggccc cgggctccac cccgacccct
     1261 gacgataaaa tcgactgcca taaacatcct tcctcactga cgcagtttct cgcacaaaaa
     1321 aaagccgcct ttctggatga ccccctggga acggccctta acctgctgaa tccgggcggt
     1381 atgctggaaa ccgcctttca gggagtgaat gcggttattg gcagcatcag taatctgttc
     1441 aaaggcgatg acgagccgcc cgcagcggaa tatatcgcgg aaggcgcgcg ggatgtgcgt
     1501 atcaacagcc agccggcgac acgcagcggg gcacgctgta cctgcgaggc cagggtggtg
     1561 aatgagccgg ggaacggggc gtttgtgtca ccggatgtgc gcattggcgg tccgttactg
     1621 gtggtcaggg atatcagaag cggcaggtcg cagataaccc tggtggcaac cgtggcgctg
     1681 atgtttcttc gcccgggcaa aatgctttct aaaatcgcct gttttgctgc cagtgtcggg
     1741 atgagcatgc tgacgcagaa ggccaccagt gcccttccgc atccggtcaa tgccgccacc
     1801 ggggccaaat acctggccga tgacgatgac ttcgatttca gtctgccggg ccactttccg
     1861 ctggactggc agcgcgtgta cagcagtcgt gacgaacgta cggaaggaat gtttggtcag
     1921 ggctggagtg taatgtatga agtcagcctc gtgtgcacac cgggtagcgc agacgaaaac
     1981 tgcatgacct ttgtgtcggg catggggcga cgcctggata tggaagccgt actgccgggg
     2041 ggcgggtttt acagccccgg tgaagggctg gcggtaaggc gtggtgagca ggggcactgg
     2101 cttatcagca gcgatgacgg gcagtgtttt ctgtttgagg ctgacccgca ccacccgcag
     2161 cgccagcggc tgaaaatgct gggcgaccgt aacagcaact gtctgaatct gtactacgac
     2221 gaccggggcc gcattgtgga aatcagcggc gaacagcagc gtccgtgcat ccgtctgtat
     2281 tacgaactgg cggcgcatcc ccggcgggtg acacagattt atcagcattt ccctgagact
     2341 gcacctttac tgctgcgtcg ttacagctat gacgaggccg gccacctgaa cggcgtgtac
     2401 gacagcacgg ggcacctgct gcgtgagttt gcttacgacg aaaaccactg catgacgctg
     2461 caccgccagc cgggcggcga agggtattat taccagtggg gctggtatga aggtccggat
     2521 gacgcggcat ggcgggtgac ggggcaccac acggacagcg gtgaacagta ccggctggcg
     2581 tgggacctgg cgcagcgcag gctgtgcgtg acggacggac tggggcgcac acgttatcac
     2641 cagtgggatg cgcagaatca ggtgacggcg taccaggacg aagccggaca ggtgacgaca
     2701 ttccgctgga gcgacgaaga acgactgttg ctggggatga ccgaccctca gggcggcaaa
     2761 tggcgctatg tgtatgacct gcaggggcat atcacggaga cgcacgaccc gctggggcgg
     2821 gtggagcagg cgcagtggca cccggtgtgg caccagccgg aaacggaggt ggacgcggcg
     2881 ggaaacacct ggcgctgtgt gtacgatgaa cgcggcaacc ttcttgccgt gaccgacccg
     2941 ctgcagcaga acacccggta tcagtatgac caccacggcc aggtggtgca gataacagat
     3001 gcgcgcgggg ggaataaata cctccagtgg aatgaagacg ggcagctcat gcggcacacg
     3061 gactgttccg gctcgcagac ggcgtggttc tacgacgaac gcacgaggct tatcagaatg
     3121 acggatgcgc agagccacag cacgcgctac gggtatgacg acagcggtca tctgacggag
     3181 gtgatactgg cggacggccg cacggtgaat taccagtcag acgcggcggg caggctggta
     3241 aagtacacca gcccgatggg gaggataacg cgctggcagc gggacgggca gggccgggtg
     3301 cgcagccgga cggacgcgat ggggcgcagg acggcattcg ggtacgacgc ttacgggcgt
     3361 ctggtcacgc tcacgaacga gaacggagaa cgctactggt tccgccatga tgtgctggac
     3421 cgtctggcgg agcagataaa cccggacggc tgccgccagt cgtaccgcta caacgcgctg
     3481 aacgcggtca cggaagtggt gttcaccggg gaccggggcg gtgaaatccg ccaccggctg
     3541 gcgcgtgatg cggcgggccg gctcacggca aaagagacag ccgatggccg tacggaatac
     3601 gtccatgatg cggcagacca gctactggaa atccgtcgcc agcggcacga aactgacgcg
     3661 ccggaagtta tccgcttcag ctacgacaga ctggggcgga tgctgagcga ggagacggcg
     3721 cagggcatcc tgacccacca gtacgatgaa ctgggcaacc ggactgccac cacctttccg
     3781 gacggcagaa cgcagcgcca tctgtactac ggcagcggcc atctccagca gataaatctg
     3841 gaccgggagg tcatcagcga gttcacgcgc gaccacctgc accgtgaagt gctcagaagc
     3901 caggggcgac tgagcacgcg gcagctttac gaccccggcg ggcggctgaa gcgcagggag
     3961 acatacagcg ggatgcgcgg ggtggtaccg gagacgttca cggaccggca gtacagctac
     4021 agtggcgagg atgaactgct gaagacgcgg cacagccggc ggggggaaac ggattacttc
     4081 tacgacccca cggggagcat cacggcgtgt cgttcggagg atgaggggta tctggcgagc
     4141 tggcagtacg acgcggcggg aaacctgctg gggaggcgcg cgggggaaag ggccacggca
     4201 gaaaacagcg tggtgccgtt caaccggctg atgtcttacc gtggtgtgca ctaccgttat
     4261 gacgaatttg gccgtgcggt ggaaaaggag ggccggagcg gaacgcagag ttaccgttat
     4321 gatgcggaac accggatggt ggaagtgacg acagcgcggg ggacataccg gtatgtgtac
     4381 gatgcactgg ggaggcggac ggaaaaacag cacatcagcc ctgacggtaa gccgtacaac
     4441 cggacaaaat ttttatggga cggaatgcgg ctggcgcagg agagcaggcc ggagggaaca
     4501 ggcagcctgt acatctacag ggaccagggg agttatgaac cgctggcgcg ggtggataag
     4561 gcgggcaaag agggcccgaa ccggatactg tattttcaca cggatgtgaa cggcgcgccg
     4621 gaagagatga cggacagcga cgggaaaatc gtgtgggaga cggggtatca ggtctggggt
     4681 aacacgatac aggagaagga ccacggcggg gtggagcaga acctgcgcta ccagggacag
     4741 tacctcgaca gggaaacggg gctgcactac aatctgcaca ggtactatga tccggatgtc
     4801 gggcggttca tggtaactga cccgataggg ttgagaggcg gtttaaatct ctacagttat
     4861 gcacctaatc ctctaaaata tgctgatcca cttggattaa ctccatgtgc tgttagtaac
     4921 caaaaagcga atcgtttatt agatagtagt gaaaccaaag ttactgttag gtctagaagt
     4981 gacgctgaac aattgtttat ggatagatat ttaggtcata attataaaaa tatgacaggt
     5041 gagtcaggcc ccagcactaa gaacttaatg gaatatctta ctggaaataa aacaaaagca
     5101 ggttcatatc attgggatga tataaaagat ccttctgtaa caaaacctcc atatagagtg
     5161 tcaggtcatg gaccaggaaa tcctgatggt gatttaccac atttacaagt tcatcaacat
     5221 ggtggaagtg ttagacatat atttttcccg agggagacat aataatgaac aatctcaata
     5281 cattattaag tggaaaggtt ttgctcaaag aaattagtcc attaatgcct ggatatagaa
     5341 catggattga aatttcattg atcgatgaat taaaaccatc ttatcctttt agattagagg
     5401 aatatgctat gatagggcat tcaccttatg ccaatgaatg taataaagat gaagctaaat
     5461 ttaaactacg aatatcatca tttttagctt ctgacataga taatgaatac gatccatcat
     5521 atgattatgt tggaaaatat gaaattatcg cttcacttaa tgagttaaaa actaggttaa
     5581 gtactcttca tgtaaacttg gagcaattta taaattcatc ggaagatgat gagtatacat
     5641 tataataaga cagaataaaa agctaggaag gttttaaatc ttcccagctt ttttaatatt
     5701 taactttcgt tcagcacact aaacatccca tcccgcatcc ccttaactac ctgtttaacc
     5761 tgataaagtt cctcccgcag ttcctgcacc tcgtcactgt cagcgatgat ggtcaggccc
     5821 ccctccgaaa tcttcaccgt gacaccccgt ccggtatcaa atccggcctc tttcagccag
     5881 tcgcccttaa ggtgcaggct ataataccgg gctataccgg ttttgcggtc ctggtggcgg
     5941 gctctgacat agccgacttt atagcggcgc tgtgttttgg aaatggtgac ttctggcgta
     6001 atatcgtgtt cagccatgat taactacctt gattagttgc ttgcggggag cgcgctttcg
     6061 taatcttgcc ctgctagcgg attaacttca caaacatcat ttaccaacgt ctacacttac
     6121 tcctgtatac cgttcaggag cagtaatgaa tctacaacga tttgatgaca gcaccctaat
     6181 ccgtatcttc gcccttcatg agttacatcg actgaaagaa catggcttaa cgcgcggggc
     6241 gcttctcgat tatcacagcc gctataaact cgtctttctg gcccattctc agcccgagta
     6301 ccgcaaactt ggcccgtttg tggctgatat tcatcagtgg caaaatctgg atgattttta
     6361 caaccagtac cgccaacgcg taattgtttt gctttctcat cccgccaacc cgcgcgatca
     6421 caccaacgtt ttgatgcacg ttcagggtta ttttcgcccg catattgatt ccacagaacg
     6481 ccagcagctg gctgcgctta tcgacagtta tcgccgtggc gagcaaccac ttcttgcgcc
     6541 gctgatgcgt atcaaacact atatggcgct ttatcctgac gcctggcttt cagggcagcg
     6601 ttatttcgaa ctttggccgc gtgtgattaa cttgcgccat tcaggagttt tatgactacc
     6661 catctggcct ggtttcgcca ggatttacgt ctgc
//
LOCUS       DADLTF010000185         6675 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.187, whole genome shotgun sequence.
ACCESSION   DADLTF010000185 DADLTF010000000
VERSION     DADLTF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.187"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..802)
                     /gene="mutY"
                     /locus_tag="JN749_003711"
     CDS             complement(<1..802)
                     /gene="mutY"
                     /locus_tag="JN749_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAY7369965.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQMLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPKFADE"
     gene            963..1682
                     /gene="trmB"
                     /locus_tag="JN749_003712"
     CDS             963..1682
                     /gene="trmB"
                     /locus_tag="JN749_003712"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAY7369966.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            1682..2008
                     /locus_tag="JN749_003713"
     CDS             1682..2008
                     /locus_tag="JN749_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HAY7369967.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            2192..2911
                     /locus_tag="JN749_003714"
     CDS             2192..2911
                     /locus_tag="JN749_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HAY7369968.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            3087..4133
                     /gene="ansB"
                     /locus_tag="JN749_003715"
     CDS             3087..4133
                     /gene="ansB"
                     /locus_tag="JN749_003715"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HAY7369969.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            4250..5257
                     /locus_tag="JN749_003716"
     CDS             4250..5257
                     /locus_tag="JN749_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HAY7369970.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(5313..6614)
                     /locus_tag="JN749_003717"
                     /pseudo
     CDS             complement(5313..6614)
                     /locus_tag="JN749_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT         1888 a         1582 c         1712 g         1493 t
ORIGIN      
        1 cttcgtcggc aaacttcggg aaacagtata aaccgcccca caatccgctc ggcggacgct
       61 gcgccagcaa tacttcatct tcgtgctgta acaggagaaa gtagccggtg cgctccggca
      121 gcatctgttt cggttttttg cccggataaa gcgaccagct attgttggtg gcggcaatac
      181 atccgttttg tagcggacag agcgaacatt tcggcttcga acgcgtgcaa atcatcgcgc
      241 ccaaatccat catcgcctga ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc
      301 tcaaactcca tagtttattc tcgacctctt ttttcccagg ccagccgctt acagcatagc
      361 agcgcgccag cacgcgtttg acgttaccgt cgagaatcgg aaagtgcttg cccagagaaa
      421 gcgagagaat cgcgcctgcg gtggaacgcc cgacgcccgg taacggcgcg acttcttcaa
      481 aggtttccgg gaatttacca ccgtgtaagg tcgccacttg ttgtgccgct ttatgcaggt
      541 tgcgcgcgcg ggcgtaatag ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg
      601 cattggcgag atcggtcacc gtcgggaagt gcgccataaa gcgttcaaaa taggggataa
      661 cggtcgcaac ctgagtttgt tgcaacatca cttctgagag ccatactttg tagggcgtct
      721 tgtcaatttg ccagggcagc gttttccgcc cgtatttatc gtaccagtcc agaacctggg
      781 ctgaaaattg cgacgcttgc atggtcaccg acttcactgt tgttgggggc aagattgcag
      841 cacagcgacg gcagggtgta aaccggaact ttccgcagcc acggccttat catgcttgca
      901 tccggcaact aactttggat aatgcccgtt ttcagaacac tttcacaagc gactaaatct
      961 ttatgaaaaa cgacgtcatt tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta
     1021 tccgtagttt tgtgcgccgc caggggcgac tgaccaaagg ccaggaacat gcgctggaaa
     1081 attactggcc ggtgatgggc gttgagttca gtgaagatat gctggatttc cccgcgcttt
     1141 ttggccgtga agcgccggtg acgcttgaga ttggttttgg catgggggcg tcgctggtgg
     1201 caatggctaa agatcgcccc gagcaggact tcctcggcat tgaagtgcat tcaccgggcg
     1261 ttggtgcgtg cctggcttct gcgcatgaag aaggtttaag caacctgcgc gtgatgtgtc
     1321 acgatgcggt tgaagtgctg cataaaatga ttcctgacaa ttcattgcgc atggtgcagc
     1381 tctttttccc tgacccgtgg cacaaagcgc gccataataa acgccgtatc gttcaggtgc
     1441 cgtttgccga actggtaaaa agcaaactgc agctgggggg cgtattccat atggcgaccg
     1501 actgggaacc ttatgcggaa catatgcttg aagtgatgtc ttctattgac ggttataaaa
     1561 acctgtcaga gagcaatgat tacgtaccgc gtccggcatc acgtccggtg acgaaatttg
     1621 aacaacgtgg tcatcgtctt ggtcacggag tatgggactt aatgttcgag agggtgaaat
     1681 aatggcaaag aaccgtagcc gtcgtctgcg taaaaaaatg cacatcgacg aattccagga
     1741 attaggattt tcggtggcat ggcgattccc ggaaggtaca tcggaagaac agattgataa
     1801 aatcgttgat gactttatta acgaggttat cgaaccgaac aaactggcct ttgacggcag
     1861 cggttatctg gcctgggaag gtctgatctg catgcaggaa atcggcaaat gcaccgaaga
     1921 acatcaggcg attgtgcgta agtggctgga agagtgcaaa ctggatgagg tacgcaccag
     1981 cgaacttttc gacgtttggt gggactaaga aagcatacgg gcgatgacaa atgcaaaact
     2041 gcctgatgcg ctacgcttat caggcctaca gagatgcacg atcgagtagg cgggataagg
     2101 cgtttacgcc gcatccggca tggaaaacgc gtactttgtt atcaatctgg ggccagcata
     2161 tgctggcctg atttgttctt gagggaagac tatgatgcgc aaaatgctgc tggcggcagc
     2221 actttcagtg acggcaatga ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga
     2281 cgatgtgatt gtcagcccgc aaaccgtgca ggtgaagggc gaaaacggca atctggtgat
     2341 cacgccagac ggcaacgtga tgtataacgg taagcaatat tccctgaatg ccgcccagcg
     2401 cgagcaggcg aaggattatc aggctgaact acgtagcacg ctgccgtgga ttgatgaagg
     2461 cgcgaaaagc cgcgtcgaga aagcccgtat tgcgctggat aaaattatcg ttcaggagat
     2521 gggcgacagc agcaaaatgc gcagccgtct gaccaaactt gatgcgcagc ttaaagagca
     2581 gatgaatcgc attatcgaaa cgcgcagcga tggcctgacg ttccactata aagccattga
     2641 tcaggttcgc gccgaaggcc agcaactagt gaatcaggca atgggcggaa ttttacagga
     2701 cagcattaat gaaatgggcg cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa
     2761 tgtgctggga agcttgggcg gtctgcaatc ctcaatccaa accgagtgga aaaagcagga
     2821 aaaagatttc cagcagtttg gcaaagatgt ttgtagccgc gttgtgactc tggaagatag
     2881 ccgcaaagcc ctggtcggga atttaaaata atcctctatt ttaagacggc ataatacttt
     2941 tttatgccgt ttaattcttc gttttgttac ctgcctctaa ctttgtagat ctccaaaata
     3001 tattcacgtt gtaaattgtt taacgtcaaa tttcccatac aaagctaagg gataatgcgt
     3061 agcgttcacg taactggagg aatgaaatgg agtttttcaa aaagacggca cttgccgcac
     3121 tggttatggg ttttagtggt gcagcattgg cattacccaa tatcaccatt ttagcaaccg
     3181 gcgggaccat tgccggtggt ggtgactccg caaccaaatc taactacaca gcgggtaaag
     3241 ttggcgtaga aaatctggtt aatgcggtgc cgcaactaaa agacattgcg aacgttaaag
     3301 gcgagcaggt agtgaatatc ggctcccagg acatgaacga taatgtctgg ctgacactgg
     3361 cgaaaaaaat taacgccgac tgcgataaaa ccgacggctt cgtcattacc cacggtaccg
     3421 acacgatgga agaaactgct tacttcctcg acctgacggt gaaatgcgac aaaccggtgg
     3481 tgatggtcgg cgcaatgcgc ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt
     3541 ataacgcggt agtgaccgca gctgataaag cctccgctaa tcgtggcgtg ctggtggtga
     3601 tgaacgacac cgtactggac ggtcgcgatg tcaccaaaac caacaccacc gacgtagcga
     3661 ccttcaagtc tgttaactac ggtcctctgg gatacattca caacggtaag attgactacc
     3721 aacgtacccc ggcacgtaag cacaccagcg atacgccatt cgatgtctct aagctgaatg
     3781 agctgccgaa agtcggcatc gtttataact acgctaacgc atccgatctt ccggctaaag
     3841 cactggtaga tgcgggctat gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt
     3901 ataaatccgt gttcgacacc ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt
     3961 cttcccgcgt accgacgggt gctaccactc aggatgctga agtggatgat gcgaaatacg
     4021 gcttcgtcgc ctctggcacg ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc
     4081 tgacgcaaac caaagatccg cagcagatcc agcagatttt caatcagtac taatcacctc
     4141 gtcccggcat cgtgccgggg ctttttcact tcagacttac gtccattgcc aattttcatt
     4201 accctaatga taatcaccgg aataaattat tccgcgcgag ggttttcggg tgaaaaagca
     4261 atggattgtt ggcacggcgc tgcttatgtt gatgactggt aatgcctggg cagatggcga
     4321 accgccaact gaaaatatct taaaagatca attcaaaaag cagtatcacg gcattctcaa
     4381 gcttgatgcc atcagcttaa aaaatcttga tgctaagggt aatcaggcca cctggtcagc
     4441 ggaaggcgat gtctcttcca gtgacgatct ctatacctgg gtcggtcagt tggcagatta
     4501 cgaactgctc gaacagacct ggacgaaaga taaaccggta aaattctcgg cgatgttaac
     4561 cagtaaagga acgcccgcgt ctggctggtc ggtgaacttt tactcttttc aggcggcagc
     4621 cagcgatcgt gggcgggtgg tcgacgatat caaaacgaat aataaatatc tgatcgtgaa
     4681 tagcgaagat ttcaattatc gctttagtca gcttgaatct gcgttgaata cccagaagaa
     4741 ctcgattcct gcgctggaaa aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca
     4801 gaaagcagcg gatgcatatt ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga
     4861 agcctttaaa aaaattcacc aacagcgtga tgagtttaat aaacagaacg atagcgaggc
     4921 gttcgcggtt aaatatgaca aagaggttta tcaaccggcg attgcggcat gtcataaaca
     4981 gagtgaagag tgttatgaag tgccgattca gcagaaacga gatttcgata tcaacgaaca
     5041 acggcgacag acttttctgc aatcacaaaa actcagccgt aaattgcagg atgactgggt
     5101 aacgctcgaa aaagggcaat atccgttaac gatgaaagtt tcagaaataa acagtaaaaa
     5161 agtcgcgatt ttgatgaaaa ttgacgatat caaccaagct aatgaacgct ggaaaaaaga
     5221 taccgaacaa ctgcggcgca atggggtgat taagtaactc atgttaagtg cctttgccgg
     5281 ataatataaa aagttatccg gcaaattatt ttttacatta gccctagtag ctgtggaatg
     5341 aacaaggata tttgcggaat gtaagtgatc atcatcaacg ctactagcaa tgcagcataa
     5401 aatggcagca atggtttgat taaatgttga atcttcacac ccgagataga acaaccaaca
     5461 aatagcgcac tacccaccgg cagtgttaac agcccaatac aaaggttggc caccatcata
     5521 ataccgaaat gtactgggtc cattcccagc tcttgtgcaa tcggcaagaa gatcggtgta
     5581 aagattagta cagccggtgt catgtccata aagatgccga caattaataa gacgatatta
     5641 atcagcagca atatgatcag tgggttatca gaaatcccca tcaatgcgtc gctgatcata
     5701 taaggaatat cagcattagt cattgcccag gacatgccca cggaaaaacc gatcaacagt
     5761 aatacaatcg aagtggtaac aaccgactcc agaatcagtt tgggtaaatg gcgccatttt
     5821 acttcccggt aaatcagcac cgagagaata aatgtgtaga caacggcaat tgctgacgct
     5881 tcagttgcgg taaaaatgcc gcccagaatg ccgcccatca caatgaacac cagaagcaaa
     5941 cttggtaagg catccagaaa cgctttacag gcctgagcaa aggttgctct ttctgaaagg
     6001 gggtagccac ggcgtttagc aataatgccg cagactatca ttaccgcaag acccattaag
     6061 atccctggca aatatcccgc cataaacagc gatgctacag aaacgccgcc agctgtcagg
     6121 gaaaacacaa ttaacacgtt tgagggcggt atcagtaatc cagtaataca agatgagacg
     6181 tttacagcgg tagaaaatgc cggatcataa ccttctttgg tctgaatggg attcagtgtg
     6241 ccaccaacgg cagcagctgc cgcaacggct gaaccagaaa tggaaccaaa catcatattc
     6301 gccaggacgt tcacatgacc aagcgaacca ggaacgcggc caaccattac ctaggcgagg
     6361 ttaatcaaac gtatcgcaat accgccgctg ttcatcagtg ttccggcaaa gatgaaaaag
     6421 gggattgcca acagggcaaa gttatccagt ccattagcca gtcgttgcga aacagtgatt
     6481 gccgcgatat cccagggaaa catcaaaagc attgaagcta cagtggcaat accgattgag
     6541 aacgaaatgg gcacaccaat aaagaccagc acaaaaaaac agccaaacag tgtaatcgcg
     6601 atataactat ccattaactg gcctcctgcg caggttgttt cagttgttga atgctttgat
     6661 taataaaaca caagg
//
LOCUS       DADLTF010000186         6365 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.188, whole genome shotgun sequence.
ACCESSION   DADLTF010000186 DADLTF010000000
VERSION     DADLTF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.188"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(38..196)
                     /locus_tag="JN749_003718"
     CDS             complement(38..196)
                     /locus_tag="JN749_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369971.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKSCRLAP"
     gene            377..2008
                     /gene="dcuS"
                     /locus_tag="JN749_003719"
     CDS             377..2008
                     /gene="dcuS"
                     /locus_tag="JN749_003719"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HAY7369972.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2005..2724
                     /gene="dcuR"
                     /locus_tag="JN749_003720"
     CDS             2005..2724
                     /gene="dcuR"
                     /locus_tag="JN749_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HAY7369973.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3295..4635
                     /locus_tag="JN749_003721"
     CDS             3295..4635
                     /locus_tag="JN749_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HAY7369974.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4713..6359
                     /locus_tag="JN749_003722"
     CDS             4713..6359
                     /locus_tag="JN749_003722"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HAY7369975.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAVIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
BASE COUNT         1645 a         1618 c         1563 g         1539 t
ORIGIN      
        1 ctatcactta aataagtgat actggttgtc tggagattca gggggccagt ctacacgact
       61 ttcgtttgag attaaataac ttgccgttgc cacgtacgca attgccagaa tgctgacata
      121 tcgcagtgtt gaaatagaca tcgatctttt cgccggtata acagcgataa cccccgtcca
      181 gtagcgcctg atccatgacc ttccctctct catcagtatg attatcgcta taagcatagc
      241 cccggaatct tcatacagca ctggcagttt ccgttgccag agtctcttcc cctatatagc
      301 caatcatttt tactcccttt gaattacccg cctcatcaga gataatgctt aagaaatttg
      361 tcacacaagg aagctgatga gacattcatt gccctaccac atattacgca aacgtccgat
      421 gaaattgagt accactgtga tcttaatggt cagcgcggta ctgttctcgg tgctattggt
      481 ggtgcatctg atttacttct cgcaaatcag tgatatgacg cgagacgggc tggccaacaa
      541 ggcactggca gtggcgcgta ccctcgccga ctcgccggaa atccgtcagg gcttgcagaa
      601 aaaaccgcag gagagtggca tccaggccat cgcggaagcc gtgcgcaaac gcaacgatct
      661 gctgtttatt gtcgttaccg atatgcaaag tcttcgctac tcacatcctg aagcccagcg
      721 tattggtcag ccatttaaag gtgatgacat ccttaatgcg ctgaatggcg aagaaaatgt
      781 cgctatcaat cgcggttttc tggcgcaggc tttacgcgta tttaccccca tctacgatga
      841 aaatcataaa caaattggcg tggtggcgat cggccttgag ttaagccgcg tgacccaaca
      901 gatcaatgac agtcgctgga gcattatctg gtcggtatta tttggcatgc tggtcggact
      961 gattggcacc tgcattctgg ttaaggtact gaaaaaaatc cttttcggcc tggaacccta
     1021 cgaaatctcc acgctgtttg agcaacgcca ggccatgttg cagtctatca aagaaggcgt
     1081 cgttgccgtg gacgatcgcg gcgaggtcac gctgatcaac gatgccgcac aagaattgct
     1141 gaattaccgt aagtcgcagg acgatgagaa actgtcgacg ctcagccact catggtcaca
     1201 ggtggtagat gtctcggaag tgttacgcga cggtacgccg cgccgcgacg aagagattac
     1261 gattaaagac cggctattac tgatcaacac cgttccggtg cgcagtaatg gcgttatcat
     1321 cggtgccatt tcaaccttca gggacaaaac tgaagttcgt aaactgatgc agcgactcga
     1381 cggtctggtc aactatgctg acgcacttcg tgaacgatcc cacgaattta tgaataaatt
     1441 gcatgtgatt ctcggattat tgcatctgaa gagttataag cagttggaag attacattct
     1501 caaaacagcc aataactatc aggaagagat tggctctctg ctgggtaaga tcaaatctcc
     1561 ggttatcgct ggttttttaa tcagcaagat taaccgcgcg accgatttag gccatacgct
     1621 gattttaaac agtgaaagcc agctgccaga cagcggcagt gaggaccagg tcgcgacgct
     1681 gattaccaca ttgggaaatc tgatagaaaa cgcgctggaa gcattagggc cggaacccgg
     1741 tggcgaaatt agcgtaacat tgcactaccg tcacggctgg ctgcactgcg aagttaacga
     1801 tgatggaccg gggatcgcac ccgacaaaat cgatcacatt tttgacaaag gtgtctcgac
     1861 aaaaggaagc gagcgaggcg tcggtttagc acttgtcaaa caacaggtag aaaatctcgg
     1921 cggcagcatc gccgtggaat cggaaccggg gattttcaca caattttttg tccagatacc
     1981 ctgggacggg gagaggtcga acagatgatc aatgtattaa ttatcgatga cgacgcaatg
     2041 gtcgcggagc tgaatcgccg atacgtagca caaatcccag gctttcaatg ctgtggaaca
     2101 gcctcgacgc tggagaaagc caaagagatt atcttcaata gcgatgcgcc tatcgacctg
     2161 atattgctcg atatctatat gcaaaaagag aacgggctcg atttactgcc tgtcctgcat
     2221 aacgcgcgtt gcaaaagtga tgtgattgtc atctcctccg cagccgatgc ggcaaccatt
     2281 aaagattcgc tgcattacgg tgtcgtggat tacctgatca aacccttcca ggcttcccgc
     2341 tttgaagagg cgctcaccgg ctggcggcaa aagaaaatgg cgctggaaaa acatcagtat
     2401 tatgaccagg ccgagcttga tcagctaatc cacggcagca gctccaacga acaggatcct
     2461 cgtcgcttgc caaaaggctt aacgccgcag acgttacgca cgctgtgcca gtggattgac
     2521 gcgcatcagg actatgactt ttcaaccgac gagctggcta acgaggttaa catctcgcgt
     2581 gtttcctgcc gaaaatacct catctggctg gtcaactgcc acatcttgtt caccagtatc
     2641 cattatggcg tcacggggcg tccggtttac cgttaccgta ttcaggcaga acactactca
     2701 ctgctgaaac aatattgcca ataacggatc gctggttatc tgtaagtaat aattattacc
     2761 gtaatgcaaa atacccctgg cacaagttac atcgttaatc agtaaaactt gtgccagatc
     2821 aaatataatt atccctccat catcgttaaa aattaatatc tcttcagatg aacggtgttt
     2881 ttaatttcaa aacgataaca aaagttaatt aactattatg tcacccgcat tatgtgtatt
     2941 tttacccaca aatgggtaga tcagattaat ctataaacct aatgatatct gccctgagaa
     3001 caaaaaatag accgataaat atcaataaga taacagcaaa caaaacatta acatctgcgc
     3061 agtacaaact ataaacccat cgccagagag tttttctctc tgaaaaagcc gcttatcaca
     3121 gtgcataaat ttgccgctgc tttaatcagc caatattcac tgtgaggtat ttgctaaagc
     3181 cggtaacgac caaacggata tttagtcagg ctctgaaaac agttcataca aaacagaacg
     3241 tgactgtgat ctattcagca aaaatttaaa taggattatc gcgagggttc acacatgtta
     3301 tttactatcc aacttatcat aatactaata tgtctgtttt atggtgccag aaagggtggt
     3361 atcgcgctgg gtttattagg cggtatcggt ctggtcattc tggtcttcgt cttccacctt
     3421 cagctaggta aaccaccagt tgatgtcatg ctggttatca ttgcggtggt ggcggcatcg
     3481 gcgaccttgc aagcttcggg cggtcttgat gtcatgctgc aaattgccga gaagctgctg
     3541 cgccgcaacc cgaaatatgt ctcaattgtc gcgccgtttg tgacctgtac actgaccatt
     3601 ctttgcggta cgggtcatgt ggtttacacc attctgccga tcatctacga cgtcgccatt
     3661 aagaacaaca tccgtccgga acgtccgatg gcggcaagtt ctatcggtgc acagatgggg
     3721 attatcgcca gtccggtgtc ggttgcggtc gtgtctctgg ttgcgatgct gggtaatgta
     3781 acctttgatg gtcgccatct tgagttcctc gatctgctgg caatcaccat tccatcgacg
     3841 ttaatcggta tcctggcgat cggtatcttc agctggttcc gcggtaaaga tctggataaa
     3901 gacgaagagt tccagaaatt catctccgta ccggaaaacc gtgagtatgt ttacggtgat
     3961 accgcgacgc tgctggataa aaaactgccg aaaagcaact ggctggcaat gtggattttc
     4021 ctcggggcaa tcgctgtagt cgcccttctt ggtgctgatt cggacctgcg tccatccttc
     4081 ggcggcaaac cgctgtcgat ggtactggtt attcagatgt ttatgctgct gaccggggcg
     4141 ctgattatta tcctgaccaa aaccaatccc gcgtctatct caaaaaacga agtcttccgt
     4201 tccggtatga tcgccatcgt ggcggtgtac ggtatcgcat ggatggcaga aaccatgttc
     4261 ggtgcgcata tgtctgaaat tcagggcgta ctgggtgaaa tggtgaaaga gtatccgtgg
     4321 gcgtatgcca ttgttctgct gctggtttcc aagtttgtaa actctcaggc tgcggcgctg
     4381 gcggcgattg ttccggtcgc gctggcgatc ggcgttgatc cggcatacat cgtggcttca
     4441 gcaccggctt gctacggtta ttacatcctg ccgacttatc cgagcgatct ggcagcgatt
     4501 cagtttgacc gttccggcac cacccacatc ggtcgcttcg tcatcaacca cagttttatt
     4561 ctgccggggt tgattggtgt gagcgtatcg tgcgtcttcg gctggatctt cgccgcgatg
     4621 tacgggttct tataaatgca ctttgcgtgc cgcccgtgac tacgcggcac gccattttcg
     4681 aataacaaat acagagttac aggctggaag ctatgtcaaa caaacccttt atctaccagg
     4741 cacctttccc gatggggaaa gacaataccg aatactatct actcacttcc gattacgtta
     4801 gcgttgccga cttcgacggc gaaaccatcc tgaaagtgga accagaagcc ctgaccctgc
     4861 tggcgcagca agcctttcac gacgcttctt tcatgctccg tccggcacac cagaaacagg
     4921 ttgcggctat tcttcacgat ccagaagcca gcgaaaacga caagtacgtg gcactgcaat
     4981 tcttaagaaa ctccgaaatc gccgccaaag gcgtgctgcc gacctgccag gataccggca
     5041 ccgcggtcat cgtcggtaaa aaaggccagc gcgtgtggac cggtggcggt gatgaagaag
     5101 cgctgtcgaa aggcgtctat aacacctata tcgaagataa cctgcgctac tcgcaaaacg
     5161 cggcgctgga catgtacaaa gaggtcaaca ccggcactaa cctgcctgcg caaatcgacc
     5221 tgtacgcggt agatggcgat gagtacaaat tcctttgcgt tgcgaaaggc ggcggctctg
     5281 ccaacaaaac gtatctctac caggaaacca aagccctgct gactcccggc aaactgaaaa
     5341 acttcctcgt cgagaaaatg cgtaccctcg gtactgcagc ctgcccgccg taccatatcg
     5401 cgtttgtgat tggcggtacg tctgcggaaa ccaacctgaa aaccgtcaaa ttagcaagcg
     5461 ctcactatta cgatgaactg ccgacggaag ggaacgaaca tggtcaggcg ttccgcgatg
     5521 tccagctgga acaggaactg ctggaagagg cccagaaact cggtcttggc gcgcagtttg
     5581 gcggtaaata cttcgcgcac gacattcgcg ttatccgtct gccacgtcac ggcgcatcct
     5641 gcccggtagg catgggcgtc tcctgctccg ctgaccgtaa cattaaagcg aaaatcaacc
     5701 gcgaaggtat ctggatcgaa aaactggaac acaatctagg tcagtacatt ccacaagaac
     5761 tgcgtcaggc cggtgaaggc gaagcggtga aggtcgacct taaccgcccg atgaaagaga
     5821 tcctcgccca gctttcgcaa tatccggttt ccactcgttt gtcgctcact ggcaccatta
     5881 ttgtgggtcg agatattgca cacgccaagc tgaaagagct gattgacgcc ggtaaagaac
     5941 ttccgcagta catcaaagat cacccgatct actacgcggg tccggcgaaa acccctgccg
     6001 gttatccatc aggttcactt ggcccaacca ccgcaggtcg tatggactcc tatgtggatc
     6061 tgctgcaatc ccacggcggc agcatgatca tgctggcgaa aggcaaccgc agccagcagg
     6121 ttaccgacgc gtgtcataaa cacggcggct tctacctcgg tagcatcggc ggtccggcgg
     6181 cggtactggc gcagcagagc atcaagcatc tggaatgcgt cgcttatccg gagctgggta
     6241 tggaagctat ctggaaaatt gaagtagaag atttcccggc gtttatcctg gtcgatgaca
     6301 aaggtaacga cttcttccag caaatcgtca acaaacagtg cgcgaactgc actaagtaac
     6361 ctctt
//
LOCUS       DADLTF010000187         6243 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.189, whole genome shotgun sequence.
ACCESSION   DADLTF010000187 DADLTF010000000
VERSION     DADLTF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.189"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..367)
                     /locus_tag="JN749_003723"
                     /pseudo
     CDS             complement(1..367)
                     /locus_tag="JN749_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(707..1261)
                     /locus_tag="JN749_003724"
                     /pseudo
     CDS             complement(707..1261)
                     /locus_tag="JN749_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1404..1526
                     /locus_tag="JN749_003725"
                     /pseudo
     CDS             1404..1526
                     /locus_tag="JN749_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1788..2354)
                     /locus_tag="JN749_003726"
     CDS             complement(1788..2354)
                     /locus_tag="JN749_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369976.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2636..2950)
                     /locus_tag="JN749_003727"
     CDS             complement(2636..2950)
                     /locus_tag="JN749_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369977.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3028..3138)
                     /locus_tag="JN749_003728"
                     /pseudo
     CDS             complement(3028..3138)
                     /locus_tag="JN749_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="JN749_003729"
     CDS             complement(3462..4139)
                     /gene="ospD1"
                     /locus_tag="JN749_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspD1"
                     /protein_id="HAY7369978.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4510..4647)
                     /locus_tag="JN749_003730"
                     /pseudo
     CDS             complement(4510..4647)
                     /locus_tag="JN749_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4729..5027)
                     /locus_tag="JN749_003731"
                     /pseudo
     CDS             complement(4729..5027)
                     /locus_tag="JN749_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5616..5775)
                     /locus_tag="JN749_003732"
                     /pseudo
     CDS             complement(5616..5775)
                     /locus_tag="JN749_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5779..6243)
                     /locus_tag="JN749_003733"
                     /pseudo
     CDS             complement(5779..6243)
                     /locus_tag="JN749_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1672 a         1388 c         1277 g         1906 t
ORIGIN      
        1 tgacagtgtt gttgcgcctt gctgccaacg aagctccact gcccatcaag ttcacagata
       61 agggcaacat cagcgtgagc gactggcgca gatgttatcc gccggggcgg gagcttttta
      121 atgtccgtat gacggtattg atgccgattt tcagcgtacg ggcagtatcg cgaactccgg
      181 caccgttgaa agccatttca gtgatttggt ttttaactcc cggtttacgg gcctcgtaac
      241 agtaagtgag ctgaaacacc cggtgacagt cacggcaacg gaatcgatca tgtccttttg
      301 ggttctgccc gtgacggtaa acctgaacgg actggcaacg ggggcaatga aagttaactg
      361 tgaccatgaa agaaccttaa aagcgggtat tataacccct aattcaacta attggaggca
      421 ttgtcatttt taatctatgg atacacttaa aaatcctctc accttacgtt aaaaaccttc
      481 tcatgtttag agttttttat tatgtttatc ataatgatat gacgatctga gtttttaaaa
      541 tcaagaagaa aagataagtt ttttaaatgc acagtgcctg tggataactt tgcgtaaatc
      601 cggaggacag atcactctga agcaagacaa cagcatctga aaacaatatg ctcacgtttc
      661 aaccacaggg cgtaagtgat cctacccacg taatatggac acaggcctaa gcgaggttct
      721 ggttttcaaa ttgttccgga ctgaggccgc cacaccaact gtgccgtcgc cagcgattgt
      781 aatcacattc gatataatta aacactgttg cccgcattat ttcccggctg ataaagtgtt
      841 ctccgtggag acattccact ttcagcgaat gaaagaagct ttccacgcag gcattatcgt
      901 agcagcagcc ctttgcgctc atactgccac gcagattatg ccacttcagc agcgcctgat
      961 aatctgctga acagtactga cttcctcggt ccgagtgaac gataacgttc cggggtctct
     1021 tacgccgcca cagcgccatc tgcagggcat cgcaggccag ttgcgccgtc aggcgtggtg
     1081 acatggacca gccaataacg gcacgtgacc acaggtcaat gaccacagcc agatacagcc
     1141 agccttcatc tgtacgtaag tacgtgatgt ctcccgccca cttctggttc ggaccactgg
     1201 cgtaaaaatc ctgctccagc agattttctg acacaggcag gccatgtgcg cggtaactga
     1261 ccgtattgct gggtaagatc aatttgctca ctggtattta ctacgtgcat cgaatgagtg
     1321 atcaagagcc gtatgacgcg agagtgtcac gtacggttcc gtaagcagcc ggagagtgaa
     1381 actctctccg gctgactcga ctcgcaggtt ccgacagcgg tgacgaacat gcagcggtgt
     1441 tgttctcgct gatcggcaca tgccgtctga acaatgtgga gccagaaaaa tggctgcgtt
     1501 acgttattga acatatccag gactgaccgg caaaccgggt acgcgatctg ttgccttgga
     1561 aagttgatct gacttctcag taaatatcaa tatgattctg acgagccgct tacctttgag
     1621 cagagaaagg ggagagggat cccacatcca tgagctagga aaatacaaaa actggctata
     1681 caacaattca atatattagg acttacttgc gaggagaaat atagcaccgc tcaggcaatt
     1741 cctgggtatt tacttttctt agtttcttta ctcgggatgt tctgtcatca gaaaggcgat
     1801 tctaaatttg taatatagtc actcatattc ccacgtaatt tctttagatc aatatattgc
     1861 acatttttct caaaaaactg atcggaaata agatttgcaa cattgcttgt tgtatcattt
     1921 ttaacgcttc tattcatccc ttcacaaagt tcgtccattt tctttttgaa ataactgctt
     1981 cctgatacaa gatgatttat ggaactttgt gcagcattga tgtctagaga aatgcgataa
     2041 tctttctcaa cctgtttaaa tatctgttta cggagattat cgccgatttt tcggctgtgt
     2101 tctgcacaaa acttatcaat acattggttc accacccggg atacgtagac ttttttttca
     2161 tcatttttcc aatctgtaat attttcaccg atttgttctg aaacagagct caaaatacta
     2221 gtatttattt tggttgatga tatttttact atccttttcc ccattgtaca gcggataatc
     2281 tcatccacct ttcctgaaaa atatcttttg tgagataaac tgattccaaa attattaaaa
     2341 gatgttccaa gcattataat cactttgtca tgtaaaatag taacattgtc attattttag
     2401 ctgattcaaa caattctgta aatgctttga gttctttact cccttggctc aatgatggac
     2461 cgtctggaat cccagagact aaactctctg agaaagagag tatgaaaaaa cttggggcag
     2521 tccctaaatg aagggaccaa cagcggtaaa ataaccggca tcattcataa tgatgccctg
     2581 ttagttgtag ttatgaagat ttacgtcatt aaaattgctg ttcgtagtgt cagtcctatg
     2641 gtctggcatc agctgaggat cgtagccgat acatcactgg cttcacgaca ttcgtgttga
     2701 ggctattcat gaaaactcca cgctgaaaac gccgttttgt ataaacggtc acggtatgcc
     2761 cggagccact gttgctgatg aattcgataa aaccttggcg tttcttgaag ctatcgttaa
     2821 tgcggataat gaaacaacga ttggtgagat tcgctctttt gccgacgccc tggacgctgt
     2881 ccggttcaat cgcaacaaaa tcaaccggca gctgagcaaa cccaaccttg cctctctggc
     2941 gctggagcat gaagtgattt ggctgggacg tagccgctga cagcttctat aaaagtatga
     3001 tcctacccac gtaatatgaa cacaggctta agtgaggttc tggttttcaa attgttcagg
     3061 actgagacct ccacaccaac tgtgccgtcg tcagcgattg taatcgcact tgatataatt
     3121 aaacactgta tatttcattt gtggagtctc cagtctgagg gggctttatg cattccgtat
     3181 tgggcactat gatgccagaa atctgcaagg ctccacacct tcctgtattt cccttcctct
     3241 atcacagtgc actttactta atagggtggg atgtgttcat tacatttatt gtgtttttta
     3301 tgattgtttt tgcgtccagt gtcgttcgtc atgggaaact ggttctatgg gtagtatcgc
     3361 tcagtctagt taactgtttg tttcttttta gaaattaagc agatcacaca attcggggag
     3421 agatccttct aagtgcaatt ctttgcagct atttagcaca cttaaaacaa ctcttgtatt
     3481 ttgtcagaaa taatattaaa catgtcttgt tcattattgt taagtctgtc tgctatttct
     3541 gtaagtttta tcccactatg atggcatata tttatatatg ccatcatagc ctcgatattc
     3601 cctttccctg cggccagaaa taatcctggt aacccctcat tatctcttac atccaatagt
     3661 ttaatcgttt cttttggtga taaatcaaaa gttttaataa aatctccata agcttgaatc
     3721 gcagcggaat gaccttcttg aagagctatg aacaaagcag attccccagc attatttcgt
     3781 gctgcaagaa cttctagttt ttcctctttg ggtatttcgg ctgccaacag cgattcaccg
     3841 tatttacgcg ctgcatcggc atttccatgc caaatcaatg aaaacaaatt atggcggtta
     3901 tactccttat tattctttgt cggaatggat atgctctgta ttttggccaa ttttctgaaa
     3961 gaaacaccac tatatccccc ccctgatttt tcttcattga tggcgtggga tatagcggta
     4021 tttgtcacgt taatgatatt atttttcatt cctttatttt cattagcagt attgctgcct
     4081 attattaggt gcatattttt gttgtttgct ggatgtaatc catagttatt tattgacatt
     4141 tttgttcatc ctttttgcta aaaaaaacaa taacctattt ttgtaatgta atagatatta
     4201 gtctattgct aatctaaaaa aaccattgcc acattgaatg gctatattca ccaataaatt
     4261 ctttcgattt cgtatattga catatcgtag atatatctta tccctaagat tcaataatcg
     4321 agtccgttta ccattctgtg caagcgcctt cactcagaag tgaccgtcaa ggcgatcacc
     4381 aaacttgata ataaagcagc tcattgccat acgccagtcc ttaaggggcg ttgtccattt
     4441 ctgtgatgct gcctagattt aactgccagc tccggcttat ctacatctaa atatgtgagc
     4501 ctaataccgc tacactttgt ccagactatg tttttctccg ggaagtgggc taaccgtctc
     4561 cataaagtga cgaacttttt tcaacagtgg ccatattgaa cggcactgat gattacgggt
     4621 tattgtctca tgaagagccg gccacatcac tgacagcagt gacttcaggc cttcagcacc
     4681 agacagcctg aaccagttaa tccatcgccc gacagattta gtaatctagg gctggttttt
     4741 cttcagctgt tgcggttcga aagtgccatt acgatcacgc agagtacgga tttccagagg
     4801 ttcatctcca gtagtgataa tcttcgttaa gcaaccattg cgggaattgc tgccagatct
     4861 aagctgattt ttctcgcacc caagatgatg agtcatatta gcattgagta cggtttcatt
     4921 ttcagcatcc aatcaaactg actgagatct tcaggatttt tgagactttt ggctagttca
     4981 ttagctaaag cccgcaactt attagatgtc aactgttttt cattcataaa ttcaccttca
     5041 tttgatatca aattaaatag ttcaacatca gagaattata caaacttatg tacaggctcc
     5101 tatgatcttc gaatcctgaa gattatagtg aaaataagtt ttcacgttga aatcccacat
     5161 agttagtata tgataaataa tcatggatga cctgattaac ctcttggggt agtgctttta
     5221 tagaagaata aatattctac taatttaata catcacctaa aatagtattg ttttttatgg
     5281 ttggttgaag gtcgtggtga tgatgttagt ccttttgaga atattccagt gccagatctt
     5341 acttataata aaggggcttg gcagtttgga taaactattg ttcttattcc actaatacaa
     5401 actttttgta aagtatatta aatatacttc atttcatact gaaatccccg gaagtattca
     5461 atcaaacaat tacaccataa aaggaagagg gggccctaat taagttgtca agcatgttta
     5521 tgatacctgc agggtataaa ttttctggct gcaagcagac acatataaaa cgtctttatt
     5581 gatcctaccc acataatatg gacacagtcg taagtgaggc tctggttttc aaattgttcc
     5641 ggactgagac caccacacca acggtgccgt cgccagcgat tgtaatcgca ctcgatataa
     5701 ctaaacactg ttgcccgcac tatttcccga tggataatag tgttctccgt ggagacattc
     5761 cattttcagc gaatgaattt tcatctgttg ctgtatccag tcatacagtg actgcatcag
     5821 tggcgcggct ctggcttttc ttgccgccag acgctgttct gctgaacatc cccggacttc
     5881 tgcctcgatg gcatacagtt caccgatacg ctgcagggct tccgtggtga tgtcggttgg
     5941 cactcttgca tgcacatcgt ggatttttct ccggacatga gccatacatg cggcttccgt
     6001 tattctgccg gattcgtaca acgcccggta accaccgtaa gcatcggcct gaagcactcc
     6061 gctgtaaccg gccagatgat tctgtggatg gatacctttc cggtccggtg agtacgcgaa
     6121 ccagaccgcc ggaggcattt ccgaaccggc gttacggtca tcacgaacgt agacccacag
     6181 ccgggcagtc cttgttttac cgctgcccgg ctcctggacc ggaacgggga tatcatcggc
     6241 atg
//
LOCUS       DADLTF010000188         6241 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.190, whole genome shotgun sequence.
ACCESSION   DADLTF010000188 DADLTF010000000
VERSION     DADLTF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.190"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            7..312
                     /locus_tag="JN749_003734"
     CDS             7..312
                     /locus_tag="JN749_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7369979.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(388..852)
                     /locus_tag="JN749_003735"
     CDS             complement(388..852)
                     /locus_tag="JN749_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HAY7369980.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /locus_tag="JN749_003736"
     CDS             complement(849..1724)
                     /locus_tag="JN749_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HAY7369981.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="JN749_003737"
     CDS             complement(1721..2410)
                     /locus_tag="JN749_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAY7369982.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JN749_003738"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="JN749_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HAY7369983.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JN749_003739"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="JN749_003739"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HAY7369984.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JN749_003740"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="JN749_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HAY7369985.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1581 a         1692 c         1548 g         1420 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgtggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcagcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatgg tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt acctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 a
//
LOCUS       DADLTF010000189         6224 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.191, whole genome shotgun sequence.
ACCESSION   DADLTF010000189 DADLTF010000000
VERSION     DADLTF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.191"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(283..987)
                     /locus_tag="JN749_003741"
     CDS             complement(283..987)
                     /locus_tag="JN749_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAY7369986.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JN749_003742"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="JN749_003742"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HAY7369987.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JN749_003743"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="JN749_003743"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HAY7369988.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JN749_003744"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="JN749_003744"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HAY7369989.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JN749_003745"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="JN749_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HAY7369990.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JN749_003746"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="JN749_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HAY7369991.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="JN749_003747"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="JN749_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HAY7369992.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..>6224)
                     /locus_tag="JN749_003748"
     CDS             complement(5324..>6224)
                     /locus_tag="JN749_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369993.1"
                     /translation="RPLKFEEFEALAPQTIYVSATPGNYELEKSGGDVVDQVVRPTGL
                     LDPIIEVRPVATQVDDLLSEIRQRAAINERVLVTTLTKRMAEDLTEYLEEHGERVRYL
                     HSDIDTVERMEIIRDLRLGEFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERS
                     LIQTIGRAARNVNGKAILYGDKITPSMAKAIGETERRREKQQKYNEEHGITPQGLNKK
                     VVDILALGQNIAKTKAKGRGKSRPIVEPDNVPMDMSPKALQQKIHELEGLMMQHAQNL
                     EFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1491 a         1649 c         1556 g         1528 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgttttta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttcggtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atcgaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acgg
//
LOCUS       DADLTF010000190         6171 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.192, whole genome shotgun sequence.
ACCESSION   DADLTF010000190 DADLTF010000000
VERSION     DADLTF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.192"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            171..818
                     /gene="fucA"
                     /locus_tag="JN749_003749"
     CDS             171..818
                     /gene="fucA"
                     /locus_tag="JN749_003749"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HAY7369994.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            846..1994
                     /gene="fucO"
                     /locus_tag="JN749_003750"
     CDS             846..1994
                     /gene="fucO"
                     /locus_tag="JN749_003750"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAY7369995.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2047..2892)
                     /gene="xni"
                     /locus_tag="JN749_003751"
     CDS             complement(2047..2892)
                     /gene="xni"
                     /locus_tag="JN749_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HAY7369996.1"
                     /translation="MRSLFLFSQPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKADRPP
                     MPEELHDEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTLAGHQATIVSTDKGY
                     CQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="JN749_003752"
     CDS             complement(2913..4280)
                     /gene="sdaB"
                     /locus_tag="JN749_003752"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HAY7369997.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4338..5627)
                     /locus_tag="JN749_003753"
     CDS             complement(4338..5627)
                     /locus_tag="JN749_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HAY7369998.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1570 a         1507 c         1644 g         1450 t
ORIGIN      
        1 agagaacttg cagttattta ctgtgattag ttgagctagg tcacaaaata taagcttgtg
       61 aatttctttc ttgttaatca ggaagaagat ggtgggtaat taaacggcta attcaatagt
      121 gtgaaaggaa caacattatt gccctgtttt gaatcagaga gaggtagcta atggaacgaa
      181 ataaacttgc tcgtcagatt attgacactt gcctggaaat gacccgcctg ggactgaacc
      241 aggggacagc ggggaacgtc agtgtacgtt atcaggatgg gatgctgatt acgcctacag
      301 gcattccata tgaaaaactg acggagtcgc atattgtctt tattgatggc aacggtaaac
      361 atgaggaagg aaagctcccc tcaagcgaat ggcgtttcca tatggcagcc tatcaaagca
      421 gaccggatgc caacgcggtt gttcacaatc atgccgttca ttgcacggca gtttccattc
      481 ttaaccgatc gatccccgct attcactaca tgattgcggc ggctggcggt aattctattc
      541 cttgcgcgcc ttatgcgacc tttggaacac gcgaactttc tgaacatgtt gcgctggctc
      601 tcaaaaatcg taaggcaact ttgttacaac atcatgggct tatcgcttgt gaggtgaatc
      661 tggaaaaagc gttatggctg gcgcatgaag ttgaagtgct ggcgcaactt tacctgacga
      721 ccctggcgat tacggacccg gtgccagtgc tgagcgatga agagattgcc gtagtgctgg
      781 agaaattcaa aacctatggg ttacggattg aagagtaatt tcgtaaagca acaaggagaa
      841 ggatgatggc taacagaatg attctgaacg aaacggcatg gtttggtcgg ggtgctgttg
      901 gggctttaac cgatgaggtg aaacgccgtg gttatcagaa ggcgctgatc gtcaccgata
      961 aaacgctggt gcaatgcggc gtggtggcga aagtgaccga taagatggat gctgcagggc
     1021 tggcatgggc gatttacgac ggcgtagtgc ccaacccaac aattactgtc gtcaaagaag
     1081 ggctcggtgt attccagaat agcggcgcgg attacctgat cgctattggt ggtggttctc
     1141 cacaggatac ttgtaaagca attggcatta tcagcaacaa cccggagttt gccgatgtgc
     1201 gtagcctgga agggctttcc ccgaccaata aacccagtgt accgattctg gcaatcccca
     1261 ccacagcagg tactgcggca gaagtgacca ttaactacgt gatcactgac gaagaaaaac
     1321 ggcgcaagtt tgtttgcgtt gatccgcatg atatcccgca ggtggcgttt attgatgctg
     1381 acatgatgga tggtatgcct ccggcgctga aagccgcgac gggtgtcgat gcgctcactc
     1441 atgctattga ggggtatatt acccgtggcg cgtgggcact aaccgatgca ctgcacatta
     1501 aagcgattga aatcattgct ggggcgctgc gaggatcggt tgctggtgat aaggatgccg
     1561 gagaagaaat ggcgctcggg cagtatgttg cgggtatggg cttctcgaat gttgggttag
     1621 ggttggtgca tggtatggcg catccactgg gcgcgtttta taacactcca cacggtgttg
     1681 cgaacgccat cctgttaccg catgtcatgc gctataacgc tgactttacc ggtgagaagt
     1741 accgcgatat cgcgcgcgtt atgggcgtga aagtggaagg tatgagcctg gaaggggcgc
     1801 gtaatgccgc tgttgaagcg gtgtttgccc tcaaccgtga tgtcggtatt ccgccacatt
     1861 tgcgtgatgt tggtgtacgc aaggaagaca tttcggcact ggctcaggcg gcactggatg
     1921 atgtttgtac cggtggcaac ccgcgtgaag caacgcttga ggatattgta gagctttacc
     1981 ataccgcctg gtaaatgcgc tgatgtgata atgccggata cgatttgcgg cgtatccggc
     2041 tcgccgttac cgtaccaacc gcaattgctg taaattgccg tcgatatgca aatcggtttg
     2101 taagcgggca atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg
     2161 ccacttttcg gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac
     2221 cagcagctgc gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct
     2281 gatccccgca agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc
     2341 gataaatggc gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga
     2401 aagtaactga cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggccagtgt
     2461 cactttgacc gccagtgtgg cggctaagtc atcggcttcg ttgccgctgg ctgaccagca
     2521 cgggacgccg cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc
     2581 cggcattggc ggtcgatccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact
     2641 gcggttttca tcatcaaaaa cggcgaccgc gtgggttggc tggctgtgca taatgagctg
     2701 atcgagcgca tgctggcagg tttcgacaca gggcgaaccc tgaacggcat gaatgcgacg
     2761 gataagattc agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat
     2821 caggcgataa gggtaacatt cgatgccgga acaggctatg gctggttgag aaaacaggaa
     2881 gaggcttcgc aaaacgaggc ctctgggaga gattaatcgc aggcaacgat cttcatcgcc
     2941 aggccgccgc gagaggtttc gcggtacttg gcgttcatgt ctttacctgt ttcgtacatg
     3001 gtttcgataa ctttatcgag gcagacgcgc ggttcgctgg tacggcgcag tgccatacgc
     3061 gcggcgttca ccgctttcac tgctgcaatg gcgttacgct caatgcacgg tacctgaacc
     3121 tgccccgcga ccgggtcaca agtcagaccg aggttgtgct ccatcgcgat ttccgctgcg
     3181 atgcaaacct gcgccgggct tgcgcctaac agttctgcca gaccagctgc cgccatagag
     3241 cacgccacgc caacttcacc ctggcaaccc acttcggcac cagaaatcga cgcgttcatc
     3301 ttataaagag aaccaatggc gctggctacc agcaggtaac gagccagtga gttagcgttc
     3361 acttcgcgga taaacttatc gtagtacgcc agcacagccg ggataatccc gcacgcaccg
     3421 ttagtcggcg cagtcaccac gcgaccgcca gcagcgttct cttcgttcac tgccagtgca
     3481 aacatgttga tccagtcaac aaccgccatc gggtcagtgg tggttttatc ctggctgacc
     3541 agcatccggc gtagtgccgc agcacggcgt ggaacgcgca gtttgccagg caacacgcct
     3601 tcggtggaaa taccgcgctc aataccgcca cgcatgactt cccagacgtt cgccaggtgc
     3661 tgttccagct cttctttact gtgcagcgcc agctcgtttt tcatcatcag gccagagagt
     3721 gacagcccgg tttcctgaca atgtttttgc agatcggctg ctgaactgta cggataagga
     3781 acttcaaccg gtgcgctatc ctgctggcca aaatgctctt catcaacgat aaagccaccg
     3841 ccaatagagt agtaagtctg gctgtaaacg actttatcgc ccgccagcgc ggtaatgcgc
     3901 ataccgtttt catgcagaga aaggttgtct gcatggaagt tcatgcactg atcaaccggg
     3961 aactccactt catgctgacc gtttgccagc atcaggcgac catgagtatt cacatcctga
     4021 ataaaaccgg ggatggaatc gatatccacg gtatccggca ggttacccgc caggcccata
     4081 ataatggaga tatctgtgtg gtggccttta ccagtcagag agagcgagcc gtacacgtca
     4141 accaccacgc gggtcacgtc tttaagcagg ttacgggcaa tcagatcgtc ggtaaattgt
     4201 ttacccgctt tcattggtcc aacggtatga gaactggaag ggccaatgcc gattttgaaa
     4261 atatcgaata cgctaatcat aggaaataca tcgcgttaaa acggaggaag cgccgcccga
     4321 aagcggcgcg aaaggactta gctgaacaga gagtagaaga ttgcggagat tgcaatcagc
     4381 cccatcacga caacgaatac gttgctgatg tgaccgctgt acttacgcat tgccggtact
     4441 ttctgaattg cgtacatcgg catcaggaac aggatcatcg cgatgattgg gccgcccagg
     4501 gtttcaatca tacccaggat gctcgggttc agggtggcaa caatccaggt cgttaccagc
     4561 atgaacagcg cagtgatacg gttcagcttg ttgatttcga tagacttacc tttaccacgc
     4621 agagatttaa tcaccatacc gttgaagcct tcacgtgcgc ccaggtagtg gccgaggaag
     4681 gatttggtga tagcgataat cgcgataatc ggagccatcc acgcgataac cggtgcgtta
     4741 aagtggttag ccaggtaaga cagaatcgag atgttctgct ctttagccgc agccaggtct
     4801 gccggagtca ggctcagtac gcagctgaag acgaagaaca ttacggtcag caccatcatg
     4861 atgtgtgcga atgccaggat cttcgagcat ttctgttctg ccatatcgcc gtactcttca
     4921 cgcttcgcaa cggcgaaaga agagatgatc ggagagtggt tgaacgagaa caccattacc
     4981 ggaattgcca gccacagggt catccacaga ccgtttccgg ttgcagatgc agtgtccaga
     5041 gacagcgttt ccagtgctgc gccgttccac tgcgggatca ggtacagagc cagcagcatc
     5101 agtacgccaa caaacgggaa taccagaata ctcatcgctt taacgatcat ctgctcacca
     5161 aagcgaacga tggtcatcat acccacgatc aggatcagcg acagaatcgc acgcggcggt
     5221 ggcgtcatac ccagctggtg agacatgaag ctttcaacgg tattggtgat tgccacgcta
     5281 taaaccagca ggatcgggta gatagcgaag aagtagagca gggtaatcag tttacctgcg
     5341 ccaataccaa agtgttcttc tacaacctcg gtgatgtctt cgcccgggtt tttaccagac
     5401 agtacgaagc gagtcaggcc gcggtgagca aaaaacgtca tcgggaacgc aaggatagcc
     5461 atgatgatca gcgggatcat accgccaaca ccggcgttga ttggcaggaa cagcacgccc
     5521 gcgccgattg ccgtgccgta aaggcccagc atccacatgg tgtctgtctt gcgccaggca
     5581 ctacgagagt ctttcgacgc aatcgtgctg gtttgagtcg tttccatcta tttctcctgg
     5641 aggaatgcaa ataattcagc ttttagccag ttcagcccgg gaaggtgcct gaccgtaaaa
     5701 atgaaatctg tctatcggcg atggcttaat catttaccgc tgtaataatt tgagggcgga
     5761 aagatacaca caagttatgt atctatcagt gatcttgatc ccaaatacaa taatctgctt
     5821 ggaagataga ttatcccaac gctttactct gtaaatcaaa agtaaaagag aatttaaggg
     5881 caatatttgc ccattaaacg acatctgact tcttccgggg cgcgcaatat atgtaggttg
     5941 aactgaaaaa atcaatactt ttagttaatc atggcgataa tttctttttc atagcgataa
     6001 aatacggttt atgaaaatag ggaaacgttt gcgttgcaac ataaattgta tggcatatta
     6061 actgtgatga tatttgtcgc gtgagggtgg taagaaacat tcatataaca aatgaaatga
     6121 gttgatgagt aagtgaccaa aaacgagaaa acccggcaac aaacgtcacc g
//
LOCUS       DADLTF010000191         6001 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.193, whole genome shotgun sequence.
ACCESSION   DADLTF010000191 DADLTF010000000
VERSION     DADLTF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.193"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..92)
                     /locus_tag="JN749_003754"
     CDS             complement(<1..92)
                     /locus_tag="JN749_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7369999.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(345..1694)
                     /gene="lysC"
                     /locus_tag="JN749_003755"
     CDS             complement(345..1694)
                     /gene="lysC"
                     /locus_tag="JN749_003755"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HAY7370000.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            2219..3868
                     /gene="pgi"
                     /locus_tag="JN749_003756"
     CDS             2219..3868
                     /gene="pgi"
                     /locus_tag="JN749_003756"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAY7370001.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            4330..4572
                     /locus_tag="JN749_003757"
     CDS             4330..4572
                     /locus_tag="JN749_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370002.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            4686..5324
                     /locus_tag="JN749_003758"
     CDS             4686..5324
                     /locus_tag="JN749_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HAY7370003.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            5321..>6001
                     /locus_tag="JN749_003759"
     CDS             5321..>6001
                     /locus_tag="JN749_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HAY7370004.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSET"
BASE COUNT         1500 a         1565 c         1542 g         1394 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg ggggagtgcc atgatggtgc cctcagtgag ccagagagtt
      121 cagtgtacgg gaattcgttt gcgagcagaa ctgataatat tcattttgcc ttctcttaac
      181 tatttatctc tttcgataat taattagaga tgcagataaa aaaattaaag gcaattattc
      241 tccgataaag acgttggtta acagagtaca ggctcgttta ttgatgagca tagtgacaag
      301 aaaatcaata cggcccgaaa tatagcttcc aggccataca gtatttactc aaacaaatta
      361 ctatgcagtt tttgcaccac ctgctcggca tcttcgccgg gcaccaggaa gcacaggtta
      421 tggctggatg cgccgtaaca aatcatgcga atgttgaacg gttccagtac gccgaatacc
      481 tctttgccaa cgccgcaggc ttttgacagg tcattgccaa tcaacgcgac cagcgccaga
      541 ccttcttcca cctccacccg gcacagtgcg gaaagctcca tcagcagaga ttgcgtcagc
      601 aacgtatcgc cagtggaggt tgaaccggtg gtatcaaggg ttaatgccac gctcacttct
      661 gacgtggtga ttaagtctac cgaaatatta tgccgcgcga ggatgccgaa aacttccgcg
      721 aggaagccgc gagaatgcag catattcagg ctgtgcaaag tgagcagagt ctgattgcga
      781 cgaagcgcca gcgcgcggaa cagcggcgga ttttcagttt tattgcacac cagcgtacca
      841 cctgcgcgtg ggtctttgct ggagccgaca aagaccggga tatcgctgcg tactgcgggt
      901 agcaacgttg ccggatgcag tacttttgca ccaaaagttg ccatctctgc cgcttctgca
      961 aacgcgattt catcaatgcg ttttgctgcg gaaactacgc gtggatcggt ggtgtagatg
     1021 cccgggacgt cggtccagat atcaacacga gatgcgtgta aagcctccgc cagcaaggct
     1081 gccgtataat cgctgcctcc acggccaagc gtcgttgtac gacctttatt ttcgctaccg
     1141 ataaatccct gggtgatcac taagccttca ttgaggcgtg ggagcagctg cagcgcggcc
     1201 agttccgcca gcgcggctac atctggctct gcacgaccaa atcggtcgtt ggtacgcatc
     1261 actttacgta catcaaacca ctgtgcctga acatcgcgtt cgcgcaggat ttcgacaaac
     1321 agcagggtcg acatcagctc gccgtggctg accagctcat ctgtcagcgc cggagacgtt
     1381 gccagcgccg ccgcttctgc cagaacagta atgttctcca gcagacgttc aatctcttca
     1441 cggataacgt tcgggtaacg cagacgttcc agaatggcaa actggatgtt gcgaatagcg
     1501 tcgagttttt cgaatcgctc gccaggttcc agcccttccg ctaaagcgac cagcagatta
     1561 gtgataccag cagaagccga gaggactact aaacgcacgt tggcatcaga aagcacaata
     1621 tcagcgctgc ggttcatggc gtcaaaatca gctacgctgg taccgccaaa tttggagaca
     1681 acaatttcag acataactac ctcatgtcag gggatccatt ttcagccttg gcacaaggga
     1741 agagcggaag acgggtgggc gcagagcgat acttcgctac tattttcacc cagaagtgct
     1801 ccaccacttg cgaaacgccc gactgcgaac gcttctggtg acaacccagg ggattcagcc
     1861 cctgtagccg atgatgaacg tggccagccg ttcaatcacc tcggcgatgc accccctcag
     1921 gtgttatcac aggactggct cctccaacac cgttacttgg gcaacgcgcc tcttctggcc
     1981 tgcgctagcg caggtagtac atttataaat aaagggtgag cggggcggtt gtcaacgacg
     2041 gggtcatgcg gatttttcat ccactcctgg cggtcagtag ttcagctaat aaatgcttca
     2101 ctgcgctaag ggtttacact caacattaca ctaacggcac taaaaccatc acatttttct
     2161 gtgactggcg ctacaatctt ccaaagtcac aattctcaaa atcagaagag tattgctaat
     2221 gaaaaacatc aatccaacgc agaccgctgc ctggcaggca ctacagaaac acttcgatga
     2281 aatgaaagac gttacgatcg ccgatctttt tgctaaagac ggcgatcgtt tttctaagtt
     2341 ctccgcaacc ttcgacgatc agatgctggt ggattactcc aaaaaccgca tcactgaaga
     2401 gacgctggcg aaattacagg atctggcgaa agagtgcgat ctggcgggcg cgattaagtc
     2461 gatgttctct ggcgagaaga tcaaccgcac tgaaaaccgc gccgtgctgc acgtagcgct
     2521 gcgtaatcgt agcaataccc cgattctggt tgatggcaaa gatgttatgc cagaagtcaa
     2581 cgcggtgctg gagaagatga aaaccttctc ggaagcgatt atctccggtg agtggaaggg
     2641 ttataccggc aaagcaatca ctgacgtagt gaacatcggg atcggcggtt ctgacctcgg
     2701 cccgtacatg gtgaccgaag cactgcgtcc gtacaaaaac cacctgaaca tgcactttgt
     2761 ttctaacgtc gatgggactc acatcgcgga agtgctgaaa aaagtaaacc cggaaaccac
     2821 gctgttcttg gtagcatcta aaaccttcac cactcaggaa accatgacca acgcccatag
     2881 cgcgcgtgac tggttcctga aagcggcagg tgatgaaaaa cacgttgcga aacattttgc
     2941 ggcgctctca accaatgcca aagccgttgg cgagtttggt attgatactg ccaacatgtt
     3001 cgagttctgg gactgggtcg gcggccgtta ctctttgtgg tcagcgattg gcctgtcgat
     3061 tgttctctcc atcggctttg ataacttcgt tgaactgctt tctggcgcac acgcaatgga
     3121 caagcatttc tccaccacgc ctgccgagaa aaacctgcct gtcctgctgg cgctgattgg
     3181 catctggtac aacaatttct ttggtgcgga aactgaagcg attctgccgt atgaccagta
     3241 tatgcaccgt ttcgcggcgt acttccagca gggcaatatg gagtccaacg gtaagtatgt
     3301 tgaccgtaac ggtaacgttg tggattacca gactggcccg attatctggg gtgaaccagg
     3361 cactaacggt cagcacgcgt tctaccagct gatccaccag ggaaccaaaa tggtaccgtg
     3421 cgatttcatc gccccggcta tcacccataa cccgctctct gatcaccacc agaaactgct
     3481 gtctaacttc ttcgcccaga ccgaagcgct ggcgtttggt aaatcccgcg aagtggttga
     3541 gcaggaatat cgcgatcagg gtaaagatcc ggcaacgctt gactacgtgg tgccgttcaa
     3601 agtgttcgaa ggtaaccgcc cgaccaactc catcctgctg cgcgaaatca ctccgttcag
     3661 cctgggtgcg ttgattgcgc tgtatgagca caaaatcttt actcagggcg tgatcctgaa
     3721 catcttcacc ttcgaccagt ggggcgtgga gctgggtaaa caactggcaa accgtattct
     3781 gccagagctg aaagatgata aagaaatcag cagccacgat agctcgacca atggtctgat
     3841 taaccgctat aaagcgtggc gcggttaatc atcatgcgtg cctgatgccg atatgtaggc
     3901 cggataaggc gtttacgccg catccggcaa ccggagcctg atgccaatat cgacattaat
     3961 gcatagtatg aggagttaaa taaccttaaa aacaatgccg acaataagtc ggcattgttt
     4021 tatcagataa atccccttgt ctgtgattta acggaaatca taccgtgagg ttaatcctaa
     4081 aatagatttt taatcgttgt ttatttcgga gaatacgcag attaattact tttgttttta
     4141 ttttaagttt atgattttta ttgttattta aatataagtt gaaacttata tttgatattc
     4201 attccaatta tcctaaaact ccatcgctaa ttccccgcgc cgtaattcgc atgctttagt
     4261 tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg
     4321 gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact
     4381 tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag agtcggcagc aacgtcggtt
     4441 tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg
     4501 ctcgcggtaa ccggtggcgg tgatggttct aataccggga ccaccacaac cacgaccacc
     4561 agtacccagt aataaagtat gtatccccaa aataatttga gtcattgcat ctgtggcttg
     4621 aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc ctctggagaa
     4681 gagttgtgaa gcgacctgca ctcattctta tctgcctgct attacaggcc tgttcagcca
     4741 cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca ggcgtacagc
     4801 tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg
     4861 ggccgcaatt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc
     4921 aggatcaagc cactcttgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg
     4981 acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg
     5041 ttgatggtgc aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg
     5101 ccaccgcacg ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg
     5161 aaaccctggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc tggcataacc
     5221 gctactggat cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt
     5281 atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt
     5341 cgcgttgctt ttgagtgtgg gagcgtcatc ggtcttcgcg gcaggaaccg tcaaggtgtt
     5401 cagcaatggc agcagtgagg ccaaaacgct aacgggcgca gagcatttaa tcgatctggt
     5461 agggcaacct cgactggcaa acagctggtg gcctggtgcg gtgattagcg aagagctggc
     5521 aacggcggca gcattgcgtc agcagcaggc gttgctgaca cggctggcag agcagggcgc
     5581 agattccagc accgacgatg ccgccgccat taacgcctta cgccagcaaa tccaggcatt
     5641 gaaagtgaca ggcaggcaaa aaatcaatct tgatcccgat atcgtccgcg ttgccgaacg
     5701 cggtaacccg ccgttgcagg gcaactacac gctgtgggtc ggaccaccgc cgtccacggt
     5761 cacgttgttc gggcttatca gccatcctgg caatcagcca ttcactccag gtcgcgacgt
     5821 ggcgagctat ctctctgacc agagcctgct cagcggtgcg gatcgcagct acgcgtgggt
     5881 cgtttacccg gacggacgca cgcaaaaagc gccggtggct tactggaaca agcgtcacgt
     5941 agagccgatg cccggcagca ttatttatgt tggcctcgca gattccgtct ggagtgagac
     6001 t
//
LOCUS       DADLTF010000192         5997 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.194, whole genome shotgun sequence.
ACCESSION   DADLTF010000192 DADLTF010000000
VERSION     DADLTF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.194"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..268)
                     /locus_tag="JN749_003760"
     CDS             complement(<1..268)
                     /locus_tag="JN749_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HAY7370005.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /locus_tag="JN749_003761"
     CDS             805..2706
                     /locus_tag="JN749_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAY7370006.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="JN749_003762"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="JN749_003762"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAY7370007.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="JN749_003763"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="JN749_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HAY7370008.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /locus_tag="JN749_003764"
     CDS             complement(4548..4757)
                     /locus_tag="JN749_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370009.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="JN749_003765"
     CDS             5041..5403
                     /locus_tag="JN749_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HAY7370010.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1567 a         1454 c         1488 g         1488 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gagcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt aaaatttttc
     5701 ccagacatgg tttctttcat atttgacatt tattaataat catcattaga atccacgaaa
     5761 aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt tttatgatga
     5821 atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag caacatagct
     5881 caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg aaataaggat
     5941 ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt tctctgc
//
LOCUS       DADLTF010000193         5975 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.195, whole genome shotgun sequence.
ACCESSION   DADLTF010000193 DADLTF010000000
VERSION     DADLTF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.195"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            85..315
                     /locus_tag="JN749_003766"
                     /pseudo
     CDS             85..315
                     /locus_tag="JN749_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
     gene            complement(584..2089)
                     /gene="glpD"
                     /locus_tag="JN749_003767"
     CDS             complement(584..2089)
                     /gene="glpD"
                     /locus_tag="JN749_003767"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAY7370011.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            2279..2605
                     /gene="glpE"
                     /locus_tag="JN749_003768"
     CDS             2279..2605
                     /gene="glpE"
                     /locus_tag="JN749_003768"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HAY7370012.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            2650..3480
                     /gene="glpG"
                     /locus_tag="JN749_003769"
     CDS             2650..3480
                     /gene="glpG"
                     /locus_tag="JN749_003769"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HAY7370013.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            3497..4255
                     /locus_tag="JN749_003770"
     CDS             3497..4255
                     /locus_tag="JN749_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HAY7370014.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(4237..5835)
                     /locus_tag="JN749_003771"
     CDS             complement(4237..5835)
                     /locus_tag="JN749_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HAY7370015.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPCGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
BASE COUNT         1356 a         1557 c         1594 g         1468 t
ORIGIN      
        1 ccaaagggtc ccgttttttt cgccatcatt tcggaaaagt gtccagaagg ggcaagggga
       61 gtataatttc gccattcaga aagaatggaa agaataatgc aaaatagaaa atggattttg
      121 acctcgctgg taatgacttt tttcggcatc ccaatactga cgcaattttt tgcagcggtt
      181 attgccatgc tgggggctgg gcttgccgct attcctgagg tttgcaattt actctttacg
      241 ccaacaattt accttctgct caatattttt atgctgacgc tgggcgcatt aatgctattt
      301 ttctcggggc gagtggggaa cgtgcctgag ttttgttacg ttgggtatga tggcgtgggc
      361 tttgcggtaa ttccctgaat taaaaagtag gttagatttt tgacaaagtg cgctttgttc
      421 atgccggatg cgacgtgaac gtcttatctg gcctacaaga ccatgcaaat tcaatatatt
      481 gcataaacca cgtaggcctg ataagcgtag cgcatcaggc agttttgtgt ttgtcatcag
      541 tctcaggcca gattgaaatc tgacctgatc accttacgtt aatttacgac gccagcgaca
      601 acttctgctg cgtatattcc accagccact gactcacacg agattgttga tccgcattca
      661 gccacatgcc ttgttttgtg cgacgccaca gggcatcgtc ggtgcggcgg acccattcgt
      721 gatccaccag gtatttcagt tccgcttcgt agaactcatg accgaaatct tccccgagat
      781 cgcttaccgt tcccgcattg ccgagcagca gctcgctgtt gctgccgtaa gtgcgcgcgt
      841 agtgacgtgc cagcgattca gtcaggaacg ggtaacggcg gcgcaggcga gcggcgtaat
      901 cgtcgcggtc gccttcaatg gcaccacccg gcagcacgct ctctttcgtc cacgccgggc
      961 caataccctg ataatacggc gtcagttttt ccagcgcatg ttctgccagt tttcggtagg
     1021 tggtcagctt accgccgaat accgacagca gcggtgcttt gccgttttca tcatggatat
     1081 caagggtgta atcacgggta atggcttgcg gcgagtcgga ctcatcatca cacagcggac
     1141 gcacaccgga gtaggtccag acgatatcgt cacggcttag ctgcttttta aagtgcgcgt
     1201 tatacacttt cagcaggtaa ttgatttcac tctcttcaat cttcactgct ttcggatcac
     1261 ctttgtattc cacatcggtg gtgccgatga tggaaaactc gtccatccac gggatcacga
     1321 acacaatgcg tttatcttcg ttttgcagaa tgtaggcctg cttttgggta tgcacgcgcg
     1381 gcaccacaat atggctgcct ttgatcaggc gaatgccata aggcgaaggc agatgcatcc
     1441 cctcgtcgaa gaactgtttc acccacgggc cggtggcgtt aaccaggccg cgcgcttgcc
     1501 agctatattt tttgccggta tcgatatctt ccgcttccac aatccacagg ccgttttcgc
     1561 ggcgagcaga ggtggcgcga gtccgagtaa gcacttcgcc gcctttacgc accaccatct
     1621 gggcgttggc gagtaccaga cgggcgtcgt ctacccaaca gtcagaatat tcgaatccgc
     1681 gcttaatttc cggttttaac actgaatttg cgccaaaacg caaaccaatt gatcccggca
     1741 ggctggtgcg tttacccaga tgatcgtaca taaacagacc aatgcgaatc atccacgccg
     1801 ggcgcagatg cggacgatgt ggcaggcgaa aacgcatcgg gaaggcgata tgcggagcca
     1861 ttttcagcag cacttcacgt tcagccagcg cctcgctgac caggcggaat tcatagtgct
     1921 caaggtagcg caggccaccg tgaatgagtt ttgaactggc ggaagaggtc gcgcaagcga
     1981 gatcctgcgc ctccagcatc agcacggata aaccgcgtcc agcggcgtct gccgcgatac
     2041 cagcaccatt gatgccgcca cctatcacaa tcagatcttt ggtttccatg ctgccctcat
     2101 tcactttcgt taaagctcat aaatgttcgt tatcgaacat attagcaaag aatcgcgctt
     2161 tgggtaacat tgaaaaaaca ttttagagtg atatgtataa cattatggcg tttatctgcc
     2221 gcttcgacgt aaactgtgcg gtaaatttgc ccacttgttt gtaaagaaag agagacgcat
     2281 ggatcagttc gaatgtatta acgttgccga cgcgcaccag aagttgcagg aaaaagaggc
     2341 ggtgctggtc gatattcgcg atccacagag tttcgctatg gggcatgcgg tgcaggcttt
     2401 ccatttaacc aacgacacgc tgggcgcgtt tatgcgtgat aacgactttg acactccggt
     2461 gatggtgatg tgttatcacg gcaatagcag caaaggggcg gcgcagtatc tgctgcaaca
     2521 gggctacgat gtggtctata gcattgacgg cggctttgaa gcctggcagc gacagtttcc
     2581 cgcagaggtg gcgtacggcg cgtaacgctt tatactgtcc ccttttgtgt ggaataagcg
     2641 acagcaacga tgttgatgat tacctctttt gctaaccccc gcgtggcgca ggcgtttgtt
     2701 gattacatgg cgacgcaggg tgttatcctc acgattcaac aacataacca aagcgatgtc
     2761 tggctggcgg atgagtccca ggccgagcgc gtgcgggcgg agctggcgcg ttttctcgaa
     2821 aacccggcag atccgcgtta tctggcggcc agctggctgg caggccatac cggcagtggc
     2881 ctgcattatc gccgttatcc tttctttgct gccttgcgtg aacgcgcagg tccggtgacc
     2941 tgggtgatga tgatcgcctg cgtggtggtg tttattgcca tgcaaattct cggcgatcag
     3001 gaagtgatgt tatggctggc ctggccattc gatccaacac tgaaatttga gttctggcgt
     3061 tacttcaccc acgcgttaat gcacttctcg ctgatgcata tcctctttaa cctgctttgg
     3121 tggtggtatc tcggcggcgc ggtggaaaaa cgcctcggta gcggtaagct aattgtcatt
     3181 acgcttatca gcgccctgtt aagcggctat gtgcagcaaa aattcagcgg gccgtggttt
     3241 ggcgggcttt ctggcgtggt gtatgcgctg atgggctacg tctggctacg tggcgaacgc
     3301 gatccgcaaa gtggcattta cctgcaacgt gggttaatta tctttgcgct gatctggatt
     3361 gtcgccggat ggtttgattt gtttgggatg tcgatggcga acggagcaca catcgccgga
     3421 ttagccgtgg gtttagcgat ggcttttgtt gattcgctca atgcgcgaaa acgaaaataa
     3481 ttccagggat ttataaatga aacaaacaca acgtcacaac ggtattatcg aactggttaa
     3541 acagcagggt tatgtcagta ccgaggaact ggtggagcac ttctccgtca gcccgcagac
     3601 tattcgccgc gacctcaacg agctggcaga gcaaaacctg atcctgcgcc atcatggcgg
     3661 cgcggcgctg ccttccagtt cggttaacac gccgtggcac gatcgtaaag ccacccagac
     3721 cgaagaaaaa gagcgcatcg cccgcaaagt ggcggagcaa atccccaatg gctcgacgct
     3781 gtttatcgat atcggcacca cgccggaagc ggtggcgcac gcgctgctca atcacagcaa
     3841 tttgcgcatt gtcaccaaca atctcaacgt tgctaacacg ttgatggtaa aagaagattt
     3901 ccgcattatt ctcgccggtg gcgaattacg tagccgcgat ggcgggatca ttggcgaagc
     3961 gacgctcgat tttatctccc agttccgcct tgatttcggc attctgggga tcagcggcat
     4021 cgacagcgac ggctcgctgc tggagttcga ttaccacgaa gttcgtacca agcgcgccat
     4081 tattgagaac tcgcgccacg ttatgctggt ggttgaccat tcaaaatttg gccgtaatgc
     4141 gatggtcaat atggggagca tcagcatggt tgatgccgtc tacaccgacg ccccgccgcc
     4201 agtaagcgtg atgcaggtgc tgacggacca ccatattcaa ctggagctgt gctgatcctg
     4261 cacggcctcc cacgtcagac caaaacgcgc caggtatttg cgtagccgat ccgcgtcatt
     4321 gacgctggct ttgccctggc gcgaaacatc aaaaagctgt cgtccggcag cagaaagcga
     4381 ctttgcctgg cggcagatag cgataacgtg ttccagttgc atacggtcga agagatcgat
     4441 gttttctgcc tctgcgccca gcaacgccgt aagcgcggag gggcggctct cctgccagtt
     4501 atagcgcaga cggtttatct catcttccac cacgtccaga gtgatgcgtc cgctagtggc
     4561 aaaggtggcc atccgcgtga cgctggcaga aagttcgcga aagttaccgc gccatgtagc
     4621 ctggggagat gttgcaaagg ccagccaggc acgccgcgct tcggtgttaa aacgcacgct
     4681 gtcaccagtg agtgtggcgt ggcgctccac ttcataatcc aggttcggtt caatatcttc
     4741 ctggcgctgg cgtagacccg gcagggtgaa ggtccagaga ttgatccgcg cgtacagatc
     4801 ttcgcggaat ttgccttcgg caaccagctg gcgcaaatcg cgcaccgttc cggcgataag
     4861 ctgaaaatca ctgctcacct ggcgatcgct gccaaacggg taaaaggttt tctcttcaat
     4921 agctttcagc agcattgcct gttcgtctgc gcccagttcg ccaatctcat caagaaacaa
     4981 cattccgccg ttggcgctgc gtaataatcc ttcacgagat tcccgcgccc cggtaaacgc
     5041 gcctttcaca tgaccaaaca gcgtcgacat cgcggtatcg ccgcgcaggg tggcgcagtt
     5101 cacttccaca aacgcgccgc taaactgatg ccgtgcctgt tttaactcga agatgcgtcg
     5161 cgccagaaat gacttacctg cgccggtcgg gccgttaagc agaatcggcg cgcgggattt
     5221 aatcgccact ttttcgatct gctcaatcat gcggttgaag tgggggttac gcgtggcaat
     5281 accggatttg aggaaatcga gcgtttgctg ccgttcctcg gcaaagcggc tggcgatggc
     5341 gttataacgg cttaaatcga gatcgataat cgtcacttca cctgggccgc acggctgttc
     5401 ttttttgcgc ggagttgaag attgtatcaa ccgggcgggt agataacgcg cttccgccag
     5461 cagaaaccag caaatctgcg cgacgtgggt gccggtggtg atgtgaatta aatagtcttc
     5521 tttttctggc tgaaactcgt aaccacgggc gaaatcatgc aggcaggcgt aaacctcttc
     5581 gaaatcccac gggttatgca gttcaatctc aacgcccacc acttctgttt ctggcgaaac
     5641 gctggcgata tcgcgtttaa gcgtttcaaa tagcgagcgc gagcgggcat cgtgcaataa
     5701 ttccagtcga tcgatgacca acgattcttg ctggcatata cagagtgacg gacgccattt
     5761 tgaccagcgc tgactgccgc gtccggcata atccagcacg gtaccgacaa agccaaaagc
     5821 cactgtttta cgcatcttag atatccttat aaaaggcgat atttcattat cggtaaatcg
     5881 gctttgcggg aaatctccgt ttacggacat aaagatataa tctttttaga aacaataaat
     5941 taaaaaatta atctgcttat ttaattttct ggcac
//
LOCUS       DADLTF010000194         5974 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.196, whole genome shotgun sequence.
ACCESSION   DADLTF010000194 DADLTF010000000
VERSION     DADLTF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.196"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..109)
                     /locus_tag="JN749_003772"
     CDS             complement(<1..109)
                     /locus_tag="JN749_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002440519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HAY7370016.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIG"
     gene            complement(366..698)
                     /locus_tag="JN749_003773"
     CDS             complement(366..698)
                     /locus_tag="JN749_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7370017.1"
                     /translation="MEQIQPLKIIKGGAEPGVWGVELLAIRYAAWIKPEFEIEVYEVF
                     KTVVRLGVGAMSRLNRIDHIINTETKAISQCASQMAKWGVGGRKRLLHVARERAANEV
                     QMYLPGMV"
     gene            complement(671..871)
                     /locus_tag="JN749_003774"
     CDS             complement(671..871)
                     /locus_tag="JN749_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HAY7370018.1"
                     /translation="MKYPTVIVNGVSVRVDEDGRYNLNDLHAAAVANGEATEQQRPSQ
                     FLRSAQIKRFIKALWNKFNHLK"
     gene            complement(1077..1229)
                     /locus_tag="JN749_003775"
     CDS             complement(1077..1229)
                     /locus_tag="JN749_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370019.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGDHFWSKSRLNISLLMLDIEHLA
                     RGKGLR"
     gene            complement(1217..1684)
                     /locus_tag="JN749_003776"
     CDS             complement(1217..1684)
                     /locus_tag="JN749_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAY7370020.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKKVSEL
                     KQATATITDMQQRQRAADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIIRDQTALRTLQEYIRTQCLR"
     gene            complement(1681..2157)
                     /locus_tag="JN749_003777"
     CDS             complement(1681..2157)
                     /locus_tag="JN749_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HAY7370021.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIDV"
     gene            complement(2141..2464)
                     /locus_tag="JN749_003778"
     CDS             complement(2141..2464)
                     /locus_tag="JN749_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HAY7370022.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            2689..2907
                     /locus_tag="JN749_003779"
     CDS             2689..2907
                     /locus_tag="JN749_003779"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370023.1"
                     /translation="MATAQFGLWRPVLISGLLQLPTAGYVATPNPAKDSCPYTSACAF
                     TTTERALANQARRLFTIIGSMLLPVVQI"
     gene            complement(3176..3799)
                     /locus_tag="JN749_003780"
     CDS             complement(3176..3799)
                     /locus_tag="JN749_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7370024.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTELWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            complement(3796..3984)
                     /locus_tag="JN749_003781"
     CDS             complement(3796..3984)
                     /locus_tag="JN749_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7370025.1"
                     /translation="MNATIQTIPELLIQTRGNQTEVARMLSCARGTVLKYNRDSKGER
                     HVIVNGVLMVKQGKRGRR"
     gene            complement(3981..4343)
                     /locus_tag="JN749_003782"
     CDS             complement(3981..4343)
                     /locus_tag="JN749_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7370026.1"
                     /translation="MSEYQFVLPYPPSVNTYWRRRGNQYYISDKGQKYRKEVQQIILQ
                     LKLDIFTKSRLRIKVIADVPDSRRRDLDNILKGLLDSLIHAGFAEDDEQFDDIRVIRG
                     VKVPGGRLGIKITELENV"
     gene            complement(4340..4630)
                     /locus_tag="JN749_003783"
     CDS             complement(4340..4630)
                     /locus_tag="JN749_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7370027.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGMK
                     PDDLIGAWACSACHDEIDRRTHNLDNKDARLYHLEGVIRTQAILLKEGKIKS"
     gene            complement(4630..5352)
                     /locus_tag="JN749_003784"
     CDS             complement(4630..5352)
                     /locus_tag="JN749_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAY7370028.1"
                     /translation="MNELINGNAIKMTSIEIAELVGKRHDNVKRTIETLAKNGVIRLP
                     QIEDCGRINGLGLNQSFCVYVFEGEQGKRDSIVVVAQLSPEFTARLVDRWRELEEAAV
                     NIPKTLPEALRLAADLAEQKMQLENQLAIAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
     gene            complement(5345..5554)
                     /locus_tag="JN749_003785"
     CDS             complement(5345..5554)
                     /locus_tag="JN749_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HAY7370029.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDE"
     gene            complement(5514..5915)
                     /locus_tag="JN749_003786"
     CDS             complement(5514..5915)
                     /locus_tag="JN749_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370030.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMVSIQLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVKNHHAVRQAKRKANTTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
BASE COUNT         1359 a         1534 c         1356 g         1725 t
ORIGIN      
        1 tgccaatgaa ctgaaatgct tcttctgcgg ctgcgttctg gttcataacc agtttgtaaa
       61 tctctaactg gaatttctgt tcttcagtca tgggaataat ctctgccatt gttggctccg
      121 tttatccgtt aaaagggata tcagttaagt tatcccgtgt agggtataag ccattatcga
      181 gaccactcat tgaatggcct ctgcaataac cgatgtcttt ccatcagtcc gccaccacaa
      241 agaatctttt ttgccataag gctggaggtt catctttcag tggctgccag tgttatttcc
      301 ccacttactg gcttgggttg tttcgcggta ctgccgtaat gcaaaaactg gattaacctg
      361 cgaaatcaca ccattccggg caaatacatt tgcacttcat ttgccgctct ctcacgtgca
      421 acatgaagca atctttttcg cccaccaacg ccccacttag ccatttggct tgcgcactgg
      481 cttatcgctt tggtttcagt attgatgatg tgatcgattc tattcagacg ggacattgcg
      541 ccaacgccga gacggacaac cgttttgaaa acttcataaa cttcgatttc aaattccggc
      601 ttaatccatg ctgcatatct gattgccaga agttcaacac cccacacacc tggttctgca
      661 ccacctttga ttattttaag tggttgaatt tgttccaaag tgcttttatg aagcgtttta
      721 tctgcgcgct acgcaaaaac tggcttgggc gctgttgctc tgtagcctct ccatttgcaa
      781 ctgctgctgc atggagatcg tttaagttgt agcgtccatc ctcatcaaca cgaacggaca
      841 caccattgac aataactgtt gggtacttca tcagtgatta ccttttagtg atgaaccttg
      901 tcacacagga ttccggccca cagaaaggca ccgattacca aaccggcatc ctcaagggtc
      961 atcctgaaag gctctgtgtt cataagtcgc gcgtgtgaag cgcgtttgtt gcagatataa
     1021 aaaagccccg cgaatgcgag gctaaatcct ggtgtttgtg atgactggct cttatctcaa
     1081 cgcagcccct taccgcgcgc cagatgctca atatcaagca tcagcaatga gatgtttaat
     1141 ctggatttac tccagaagtg atcacccccc tgtctacaga gccagatgtg aaggatgatg
     1201 agtaaaatta tcgctatcat cgaaggcatt gcgtcctgat gtattcctga agcgttctca
     1261 gtgctgtttg gtcgcggata attccgtccc ggataccgag aacgtttcgt ccagcaactg
     1321 gagagagttc gacggtggca tcattgccca tgccggaggc gctggaggtt tcggctgagg
     1381 atggcacagg gcattttcct ttgacgagca ccctgccacc attatcaagc ttgcgccgaa
     1441 gagcatcatt ttcagctttc gcatcagcta actccttcgt gtatttagca tcgagtgcat
     1501 cagcagcacg ctggcgttgc tgcatgtcag taatggtggc ggtcgcctgc ttcagctcac
     1561 tgactttttt atctcgctgc tctttgtagg tcatggcgtt atcacggtaa tgattaaccg
     1621 cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg gtgactctgc
     1681 tcatacatca atctctctga ccgttccgcc tgcttctttg aattttgcaa tcaggctgtc
     1741 agccttatgc tcgaactgac cataaccagc gcccggaagt gaagcccaga tattgctgca
     1801 acggtcaatt gcctgacgaa tatcaccgcg atcaatcatc ggtaaagcgc cacgttcttt
     1861 aatctgttgc agtgccacag cgtcctggct tttcggagag aagtctttca ggccaagctg
     1921 cttacggtaa gcatcccacc aacgggaaag aagctgataa cgtccggcgg ctgttgattt
     1981 tagttttggg tttagtgtga caagtttgcg aggatgatcg gagtaatcag taaatagctc
     2041 tccgcctaca atgacgtcat aaccatgatt tctggttttc tgacgtccgt tatcagttcc
     2101 ctctgaccac gccagcatat cgaggaacgc cttacgttga ttattgattt ccaccatctt
     2161 ctactccggc ttttttagca gcgaagcgtt tgataagcga accaatcgag tcagtaccga
     2221 tgtagccgat gaacacgctc gttatataag cgagattgct acttagtccg gcgaagtcga
     2281 gaaggtcacg aatgaaccag gcgatgatgg cgcacatcgt tgcgtcgatt actgtttttg
     2341 taaacgcacc gccattatat ctgccgcgaa ggtacgccat tgcaaacgca aggattgccc
     2401 cgatgccttg ttcctttgcc gcgagaatgg cggctaacag gtcatgtttt tctggcatct
     2461 tcatgtctta cccccaataa ggggatttgc tctatttaat taggaataag gtcgattact
     2521 gatagaacaa atccaggcta ctgtgtttag taatcagatt tgttcgtgac cgatatgcac
     2581 gggcaaaacg gcattaggtt gttagcgcaa cctcatgccg cccactttca cgaagatcat
     2641 gtgtagaagg ccgcagcgta actatcactg atgaattcag gatagtcagt ggctacggct
     2701 cagtttggat tgtggcgacc ggtgctgatc tccggtttgc tgcaactgcc tacagctggc
     2761 tacgtggcca caccgaatcc agcgaaagat tcttgccctt acacatcagc ctgtgcattc
     2821 accacaacgg aaagagcact ggctaaccag gctcgccgac tcttcacgat tatcggctca
     2881 atgctcttac ctgttgtgca gatataaaaa atcccgaaac cgttatgcag gctctaacta
     2941 ttacctgcga actgtttcgg gattgcattt tgcagacctc tcagcctgcg atggttggag
     3001 ttccagacga tacgtcgaag tgaccaacta ggcggaatcg gtagtaagcg ccgcctcttt
     3061 tcatctcact accacaacga gcgaattaac ccatcgttgg gtcaaattta cccaacttta
     3121 ttcaataagt caatatcatg ccgttaatat gttgccatcc gtggcaatca tgctgctaac
     3181 gtgtgaccgc attcaaaata ttgtctgcga ttgactcttc cttgtggcat tgcaccacca
     3241 gagcgtcata cagcggctta acagtgcgtg accatgtggg ttgggtaagg ttggggatta
     3301 gcatcgttac agcgcgatat gcggcgcttg ctggcatcct tgaatagccg acgcctttgc
     3361 atcttccaca ctctttctca acaactctcc cccacagctc tgttttggca atatcaaccg
     3421 cacggcctgt accgtggcaa tctctgcatc ttgcgcccgg cgtcgcggca ctacggcaat
     3481 aatccgcata agcgaatgtt gcgagcactt gcagtacctt tgccttagta tttccttcaa
     3541 gctttgcaac gccacggtat ttccccgata ccttgtgtgc aaattgcatc agatagttga
     3601 tagccttttg tttgtcgttc tggctgagtt cgtgcttacc acagaatgca gccattccga
     3661 atccggcttg tgattgcgcc atccccatag cagccatcac atcagtaccg gaaagagagt
     3721 cagaagccgt ggcccgtggt gagtcgctca tcatcgggct ttttggcgaa tgaaatttag
     3781 ctacgctttc gagtctcatc gtcttcccct cttgccctgt ttgaccatca ggacgccgtt
     3841 aactattacg tgacgctcgc ctttgctgtc tcggttgtac ttgagcactg ttcctcttgc
     3901 gcaggaaagc atcctcgcca cttcggtctg attgcctcgt gtctggataa gaagctctgg
     3961 tatcgtttga attgtggcgt tcatacgttc tccagttcgg tgatttttat tccaagcctt
     4021 ccgcctggta ctttcacacc acgaattacg cgaatgtcat cgaattgctc gtcgtcttcc
     4081 gcaaatccgg cgtggataag ggagtcgagt aaacctttca ggatgttatc gaggtcgcgg
     4141 cggcgggagt ctggaacgtc tgcgatgact ttgatgcgga gtcgtgattt ggtgaaaatg
     4201 tctaacttga gttggaggat tatttgctga acttcttttc ggtatttctg gcctttatcg
     4261 ctgatgtagt attggtttcc ccgtcttcgc cagtaggtgt tcaccgacgg cgggtatgga
     4321 agcacaaact gatattcgct catgacttaa tcttcccctc cttcagcagt atcgcctgcg
     4381 tcctgatcac gccttcgagg tggtaaagtc tggcgtcttt gttgtcgaga ttatgggtgc
     4441 gtcggtcgat ttcatcgtga cacgcgctac aagcccatgc gccgatcagg tcgtcaggct
     4501 tcattcccgt tccgcaaatt ccagccatcc ggtaatgtgc cagaactgta gtttcaggat
     4561 taccattgca tacgccgtaa atacgtacct ggcattctct gccgcgtgct tctttgcgta
     4621 gattagccat taagcagcct cccctgttac tttcagcatt ccattatcga gcagctttct
     4681 ggtcagccac tgttgaccac gcccggtgat ttttgtggtg aacgatatct gtattccgtg
     4741 atttgtgttg accgctgttt ctttcactgt gaaatagccg cgatccatat attcctgcat
     4801 tggcacattg cgccgggaac ctgaagcaat aaggattttg tgatcgcgca tccacgcaaa
     4861 cagtttgttt ggaccaattc caacaacctt tgcaaagttt ccaatcaaaa ttccgctggc
     4921 ctcgccaacg cgatcggcaa actcaacttt aggtgcggca attgcgagct ggttttccag
     4981 ttgcattttc tgctcagcaa gatcagcagc aaggcgcaac gcttccggta gcgttttggg
     5041 gatattaacc gcagcttctt caagctctcg ccagcggtca acaaggcgag cggtgaattc
     5101 cggcgacaac tgggctacaa cgacaatact gtctcgcttt ccttgttcgc cttcgaacac
     5161 atacacacaa aaactttgat ttaagcctaa cccattgatt cttccacaat cctcaatttg
     5221 aggaagccgg ataacaccat ttttagccag cgtttcgatg gtacgtttca cattgtcatg
     5281 acgcttacca accaactcag cgatttcaat gcttgtcatt ttgatggcat tgccatttat
     5341 taactcattc atcgtcttct tcctcgtaca ttgagctatt cggatcgctc atcagttctg
     5401 cgcagcagtg ctcacacacg tgaacttcca gcacatgcag cttctgaccg caattagcgc
     5461 acgttaaagc tcgctcgacg ctttctttct ggtattgaag ggattgggat gggctaagca
     5521 tggctttcac cattaaaaag tcgcttgtaa gcatcgatgt ctcgttttgc ttcaccgagc
     5581 tttcgtctta attccatgtt ttctgattca agcttttcca tgtcttgttg gtatcgatcg
     5641 cggtgttctt tccatgcttt tcgatacgcc ttcatgtatg tcgtattggc ctttctcttt
     5701 gcctgacgaa ctgcgtggtg gtttttcaca aaccagtcag ggtcgttaaa tgctgctctg
     5761 gcgcaggtat accaataatt tgttgcctcc ctgtttagcc aataaatact gataaatggc
     5821 aactgtatcg acaccatttt tcgttgagac tctttctcgc caaacatgtg cccttttttg
     5881 atgctaaggc caaatccagg ttgaattaaa agcattgtca tttcctcgca cgatgaaccg
     5941 ccccgggaat cctggagact aaacttcctg agaa
//
LOCUS       DADLTF010000195         5954 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.197, whole genome shotgun sequence.
ACCESSION   DADLTF010000195 DADLTF010000000
VERSION     DADLTF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.197"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1110
                     /locus_tag="JN749_003787"
     CDS             <1..1110
                     /locus_tag="JN749_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HAY7370031.1"
                     /translation="WPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMA
                     VAIQHGSFIEDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQ
                     ALNAEIQKGEEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRD
                     PFDWAEKNLQEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVF
                     IDNYAAIPSESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFID
                     ARGQRPLKVKDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHD
                     QPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(1122..1934)
                     /locus_tag="JN749_003788"
     CDS             complement(1122..1934)
                     /locus_tag="JN749_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370032.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(1938..2723)
                     /locus_tag="JN749_003789"
                     /pseudo
     CDS             complement(1938..2723)
                     /locus_tag="JN749_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
     gene            complement(2720..3388)
                     /locus_tag="JN749_003790"
     CDS             complement(2720..3388)
                     /locus_tag="JN749_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HAY7370033.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(3452..4090)
                     /locus_tag="JN749_003791"
                     /pseudo
     CDS             complement(3452..4090)
                     /locus_tag="JN749_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
     gene            complement(4103..5954)
                     /locus_tag="JN749_003792"
                     /pseudo
     CDS             complement(4103..5954)
                     /locus_tag="JN749_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
BASE COUNT         1511 a         1597 c         1369 g         1477 t
ORIGIN      
        1 tggcctgatg aagaagaagc aaccagaacg tattttccca gcccgtggtc aggcctgatg
       61 gaagcaaagg tcgatgcgtg taacgtgggt attatgggaa catccttgag cggactggat
      121 gcggcaatgg cagtggctat tcagcatggt tcgttcattg aagatgataa acaacacgtc
      181 gtttttcacc gcgataacgc aagtgaaaag ctaaatatta cgttgatgtc gcgcacgggt
      241 attttacccg aagccgattt ctattgccct attccctgcg agcccttaca catcgtcacc
      301 gatcaggcat taaatgctga gattcaaaaa ggtgaagagg gccttttgga tcgggtattt
      361 agattgatag tagaggaaat caagtttgct gatccagact ggagccaacg catagcctta
      421 gagagcctga atgtcgattc ctttgctcaa gcctggtttg ccgagcgcaa acaacgcgac
      481 ccatttgact gggcagaaaa aaatctccag gaagtcgaac gcaataaacg agaaaaacat
      541 actgttccct ggcgttatgt cattctgcgc ctgcatgaag ccgtacagga aattgttcct
      601 catctgaatg aacacgacca taaacggttc agtaaaggcc ttgccagggt tttcatcgat
      661 aattatgcgg caatcccttc agagtctatt cgtcgactac tggccttacg tgaagcgggg
      721 atcattcata ttctcgccct cggtgaagac tacgaaatgg aaattaatga gtcgcgcacc
      781 gtcctgaaaa cggaagacaa cagctactcg tttgacgttt ttattgatgc ccgcggacag
      841 cgtccgctta aagtgaaaga tattcctttc cctgggctac gcgagcaatt acagaaaaca
      901 ggggatgaaa tccctgatgt tggcgaagat tatacgttac agcaacccga agatattcgt
      961 ggacgcgtag cgttcggcgc gttgccctgg ctgatgcacg accagccttt cgttcaggga
     1021 cttacggcat gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa gcctgcatcc
     1081 cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct atcagcactc aacaaactga
     1141 tgctgttcat taagacgata gcggactccg gttcgaatat tgttttcacc ttcaatggcg
     1201 acggcgaagc gaactctttc gccgtcatga tatgccagtg cggcgcttcc ccctagcccg
     1261 agaataattg agtcgaccac gcccgtgctc actaccgttg aattttcacc gctggcaatg
     1321 atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct ggcgatttgc
     1381 accaggtcac cattactggc gacatgacac cgttcaccca atgtacaaac gcgtactcgc
     1441 attccggtat tcgcaatccg actactatct ccagcgctgc ttatccgcga agaattaccg
     1501 gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa gccgacactt
     1561 ccaatccgca cgctataacc catactggca atttgcgcgg catacccagc ggttgcgatt
     1621 cgggcgttat cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc aaactgactg
     1681 caaaatggta tctctccggg gtctgtggcc agtttttgca tagaggaaac ttcctgatgc
     1741 gcgaagtttt catccgccag ccattttttc cacgcatact caaccagact ctccgcccag
     1801 tccgtatatc cttcctcaat cagtgcatga cgaatatcgg cataactacc gccgcgtggg
     1861 aaatggcgca ggaaccagcg atacatcacc gctccgatac gccattctcg caggtctgct
     1921 cgtgtaatga tcattcgtta gtgccggtga tagccatcga ccatgctttt aaaggtttcg
     1981 tgtggcgtac gccccgtggt gcaaagataa agatgaccga agataaaaaa gaggcttata
     2041 aatgccagag caaaatgcgc ctgcaataac cagtatctta cgccaggaaa cacatctccc
     2101 acggcttgcg gatagagaca caacagcccc gtcaatagta acaacggcag caatccatac
     2161 atgacaccaa cataggcgac ctgctgtaag ggattaaatt tagactgtgt tgttgccggg
     2221 aaaggatgtt cttccccctg cataatgcca aacaaataaa atcgcgtttg ttttgccgct
     2281 cgttccagcc acccctgacg acgaatgcga tagtggtgac cattatcccc aacggcattg
     2341 atcagcacaa agccgagcca gcatgccagt aacaaaaatc agcaaacttc atgcaccgca
     2401 accagacttt taaccgcagt tgcgcccacc atcgcaaagt gatttatcag cccacttgcc
     2461 agcaacaata caaagagtaa cgcattcgac cagtgccata atctgaccgc tttgctgtaa
     2521 agataaacct tctcaccatg atcggtggct gatttcttca cgccacgagc acgaagcaag
     2581 gcgtgcagcc ccagcaccaa ccacatccca accagcaata ctccggcaat gatcaaccac
     2641 accggccaaa attcgggagt gaactgtggc acatagttcg ctaactggct ctgaaactgt
     2701 tcagcatgtt gcgacgggtt catacatttt cctttttaat caaatgttga ccgaaccggc
     2761 gatacagatg cggttttccg gcaccaggta gctgatattg atagtattta ttatcctgca
     2821 accacgcctg aatttctggg ctatcttcgc gaccaaagat caatgcgtgt tccggacagg
     2881 cgctgacgca aatgggcgga aagccttttg ccagtcggga ctcagcgcag aaatcacatt
     2941 tatctgccac tttagtgaca gggttaaggt agcgaacctg gtacggacaa gcgccaatgc
     3001 aataactgca gccgatgcac tgcgattttt ctacccgcac gatcccctgc tcatcacgcc
     3061 acgatgcccc cgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc tgacatgact
     3121 ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt gactggaatg tgtgctatcg
     3181 ataaacggct tccctgagcg gggacgtggt tagttttacg gcatgcgcgg gcgcaaatat
     3241 tacagccatt gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc ttttcttgcc
     3301 tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa gattactgtt cccatcccca
     3361 gaacaaattt gcgtcgagtg aaggacatct gattatcccc taatgcagtg gctgcaactg
     3421 tccaccctat ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc tctacctcga
     3481 accattcgca taagccataa gccttgccat aggtcataaa tcgctcgcta aacaagtaca
     3541 acaccgccgt cgatgactcc acctgccgta tatcggcata ttcaggtgca acatccatcg
     3601 cggcggtcgc ggcttcaatc tgcgcttcct agaagtaata tggcgcttgc atcagcatgg
     3661 cgactttgta aggacgagga tacgtctggc actcaaaacg aacggcgtgt gcaatggcgc
     3721 gacaaatgtc ctgctccaca acttgcaaag acatcgccgc gtagttttca ctcatggcct
     3781 gcgttgagta atagtaatca tcctgacttc ccgtcataca agcgatatcc gcgtagtctc
     3841 cgttctgctt aatgctttcc agcaaaactg acagttcctc ttctgcaacc gaatagggtg
     3901 gcgagagaaa cacggtgcgc gccaccagct gcccagccgc agaatgctgg cgaatgaact
     3961 cagccaggat ttcagcaggt tctggttcgg ttaccgtatc aacggggtga cgcatcccct
     4021 gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt cgctaacggc aattcatcct
     4081 gatggttcat cttacccatt ccttacgcag gcagtagatt gtgtgctgcc agatcggcgg
     4141 caatatcttc cagccccaga cgctgcaatg tttcgcgggt tggacaacca agctgtgggt
     4201 cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca cgatccattt
     4261 tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaaaacc caggaacaga
     4321 taagatcgtg ttcattacgc atatctttgg tttgcatcgg ttttaccgtg taggcacgat
     4381 gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc tcttcgccgg
     4441 tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta cggcttttca
     4501 gcggcgaaac ggtcattggc cagacccagt tgcacagggt gacggcgtta tgcaaacaga
     4561 cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc gtgtagtttt
     4621 tggtttcatc gtaagcatct tcagaaccaa aaagttcttt cgccacttca cgttgcagtt
     4681 tcaatggcaa gccggaacca ataaagttga tatgggtatg cgtcatgcag tcacggttga
     4741 acatacagtt aacaatgaaa ccgacctgcg ctgacgcttc attggcatgg tgaaccggat
     4801 agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttta cccaaattcc
     4861 agcgttctgc gatggcatat gaaccataag ccaggtgact cagctcaccc acacgatgcg
     4921 ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt tgatcccagc
     4981 gaatttctgc atactcttca gctggcagaa cacgcttgaa cacacctttg ctgtaacaat
     5041 aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca tcgaacaaat
     5101 tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa tctttcggcc
     5161 cgttcgggaa gatggtggta tggacaaagt ttgctacaca agtgttacca cctgtgctgg
     5221 gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg atcgggcaag
     5281 agtggcagcc gctcattttt actgtgtatt tctctgccgc cggtcctaag tcaaaaacgg
     5341 atttataggt acgaaagccg accgtatttt gattgcctgg cggaatttca cccgtttcaa
     5401 tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccag cgtgacttgg
     5461 gatctgaata ctctgcccac gattgtggcg tacttggcac gacatggttg ttattcgcac
     5521 caataagttc agtcatcatg taatcattga gacgcttcat ctcctgacga tcggcaatgt
     5581 tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc attattgcgc
     5641 cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga ggaataaggt
     5701 tttccccagc ctgaccaata gccgccacac aggtttcccg actggtcaat cgacaaattt
     5761 cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc aggctaactt
     5821 tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata ataatgacgt
     5881 cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc catatgggca tcgcgactaa
     5941 atttcctttg gtaa
//
LOCUS       DADLTF010000196         5905 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.198, whole genome shotgun sequence.
ACCESSION   DADLTF010000196 DADLTF010000000
VERSION     DADLTF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.198"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(220..588)
                     /locus_tag="JN749_003793"
     CDS             complement(220..588)
                     /locus_tag="JN749_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370034.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(1082..2682)
                     /locus_tag="JN749_003794"
                     /pseudo
     CDS             complement(1082..2682)
                     /locus_tag="JN749_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2740..3337
                     /locus_tag="JN749_003795"
                     /pseudo
     CDS             2740..3337
                     /locus_tag="JN749_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075019.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            3340..5610
                     /locus_tag="JN749_003796"
                     /pseudo
     CDS             3340..5610
                     /locus_tag="JN749_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            5621..>5905
                     /locus_tag="JN749_003797"
     CDS             5621..>5905
                     /locus_tag="JN749_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HAY7370035.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVD"
BASE COUNT         1572 a         1459 c         1504 g         1370 t
ORIGIN      
        1 acttaagcca atgtagtgag tagacgagct actaactgag atgactgaag aacgctaacg
       61 agctggaggt cgatacacga ggtctgacag aggcggtcac atgccttgat tacttaggag
      121 agatacttaa tgtcggttga ccagatgctc gcatgttctg acgcatcaga ctcagaggcg
      181 gaggctggtc tccctatagt gatagtttag ggtccgtaat catgaaccag ctttactggc
      241 gtggtactgg acggatttac tggcgctgac gacggtagca tagacgtagc gagcgtagcg
      301 agttgaaacc ataggttcac ccaaggcaac acgcttacgg ctcagggact cctgcacagc
      361 gcttacagtt aatgtgtcgt gcgttagtag ttgggtcaga atcaagtatg ggctgacagg
      421 cttggtattg cttgccattg attcgttcag gcgaccagca ccagtaagga catcatccag
      481 ccaaccactc aggtcgccct gaccatcgaa tgacagcagg tccccgcagt gctgctgcaa
      541 gtaggctttg gtgaacgtgt tgtcgtgagt tgcgattggt cgtgccatag agtatctcct
      601 gtagttgaat tagttggttc ccctatagtg ataatcaagt ccatcactgg tgatgcatac
      661 agtagcgcga caggatgtga ctcacaggct ggactggatg tcacggatta aggcgaccct
      721 aggaattaag gaagtaaata taaagagggg gaccggacgt gactcacagg ttgaaccaaa
      781 tgtcaaggac taaagacgcc tcaggaatta aggaagtata tataaagaga gaccagaggc
      841 gactcacagt taaacaggaa tgtcaagagg agaccatagg aatttggagg gactggagtt
      901 taggacgcag tttaaataag accacagaca gacttagagt cggacagccg aaggcggacc
      961 agtgggccta tagtcaggag ggattatgat tattatcacc ctccctaact tcagtcagac
     1021 ttcagtgacc caaggtctca accatcaagc cgtatatggt ctccaccttg ggtctcccta
     1081 cttaaagtaa agctgtctta agcacatcag ccagcctacc cacagcgtgt cctttcccgt
     1141 acaggtcata cgtaattgac tgactgctgt gacctaagat gccttgagcg tccaccagat
     1201 tgactccacg ctctttcatt cgggtcgcca gccaatgtct cagactgtgg agagagacat
     1261 cctgagagtc tcctagggcc tcagtcagga cccttgagtt aaaccagcta gagtaggcgc
     1321 tcggagtcat cccgaagaga ggaccatcat caggctgcat gtctacccat ttgaggaaag
     1381 actcaaggtc gaacccatga acgccatcgg tcagcggcac aagtcggact gaatgcttgt
     1441 tcttcacaga cttaccgtcc ccatcttcgt ttatatcgat acagaccagt ccattgtcta
     1501 aggttacaat gtcacgttta gtcaggtggc agacctcagc agaccgagcg ccagtaacta
     1561 cagaaaccag agacgcccat ctacgggcct cagaggtcgt gtgagccgtg tgtcgtgtga
     1621 actgatactc agcctcaaca cggaccagaa gtctctccag ctcctcgtct gtgaacgctc
     1681 tgcgcttgga gtcgatatcc ttggtcagct tcatccgttc aatagggttg cggccctcaa
     1741 gttgcccatt catcagaccg taatcaagac acatcttcgc cttagtcagc agcttgttaa
     1801 tcgtcgacgg tctcacctca gaagccagca tagcgtccct gacagccagc cactcagagc
     1861 gggacatcag gtccatgtct tcaggtaaga accgagagac ggtattcagt gaggacgaca
     1921 ggtccttgta gctggatgac ttcagggtct gggccttctc agccagcagc gaggacacta
     1981 ggcttgaaaa aactacagat ggttggcgct cctgtgtgaa aactgctggc tatccccctg
     2041 ctcattcttc agaattgacg tagagtcgcc tatagttgag taatcagtat ggttattatc
     2101 atcaatcagc ttaatcagac cagaggtatc accagtgtct accctctgct gtgctgcggt
     2161 caggacttca agcgctaacc ttatgccctt ctgctggtcc agactcaagg cttgcgtagc
     2221 ggctagctcc ctcaggttgg acttctcgtc caccaaagta gcgacctcaa gtccattcca
     2281 gtaatcgtca ctagcgtctg tcaggaactg ctcagccata gaccggagat gctctgtaag
     2341 ctcctgtagt gagacttcag gcctatccaa catgaaggcc ttagcggtgg ctgcaagctc
     2401 tctctgacgc attgtggcta ctctcctgtc tgtgctgcgg gtcgatactg aaagaaacgg
     2461 tgtggtctgt cccttgagtc tgaagcggat agtatatcga ccgttagctc tacggactaa
     2521 ctgtggcgta gcgagccttg cgagcaagtg cgggactgca aggtccttga tttgtgtcag
     2581 ccttgtgtca ggacttgagg tgtagacttg ctgatacaga tagggagacc cagagaaaga
     2641 actggatgca tatacagtat tatcatggtt aatgtcagtc atatcaggac cttaagtagc
     2701 gttgagtcaa ctacttcaag tccatccatc cgtctgacag tggagtccat ctggaactac
     2761 gcgggaaata ccctcgttaa tcagcattct gacatcaaca aaacgcatga aattctgcag
     2821 gacgaatcga aatgcgaaat gattgtggtc atcgaaaact ttatgacctc atacggcaaa
     2881 atatgccgac attctgctgg cagacctgat gaccgttgag caggaagata ttattcctaa
     2941 cgactacgcc ggtaacatgg gatatctcat tttcctccag ccagtcacca gcgaaaaatt
     3001 cgaacgtaaa ccgatttact ggatcctgag tgaagtcgcg aaacgtcttg gacctgacgt
     3061 ctatcaaaag ttcacagaag gtcgcacgca ggaacaatgg ttacaacatc tgtacgccaa
     3121 aatgcttgcc aaagatccgg cgttaccgtc ttacgacgaa ctgaaaaaaa tgggtatcta
     3181 taagcgtaaa gatcccaatg gccattttgt cgcctacaaa gcatttcgtg acgaccccga
     3241 ggcaaatcca cttaaaacgc cttccggtaa gattgaaatt tattccagca agctggcgga
     3301 aattgcccgt acctgggaac tggaaaaaga tgggtagaag agaaaacggt ctggagttcc
     3361 tgcaccgtta actgcgggag ccgctgtctg ctacgtttgc atgtgaaaga tgacaccgtg
     3421 tactgggtgg agtctgatac gacaggtgac gacgtttacg gtaatcatca ggttcgggca
     3481 tgtttacgcg ggcgctctat ccgccgacgg atgaatcatc ctgacaggtt gaaatatccc
     3541 atgaagcgcg tcggcgagcg cggtgaaggt aaatttgaac ggataagttg ggacgaagcc
     3601 ctggatacca tcagtgataa tcttcggcgg atcctgaaag attacggcaa tgaggctgta
     3661 catgtcctgt acggaacagg cgtagatggc ggaaatatca ccaactcgaa cgtcccgtac
     3721 cgtctgatga actcttgcgg gggttttctc agtcgctatg gcagctacag taccgcacag
     3781 atcagtgccg caatgagtta tatgttcggt gccaatgatg gcaacagccc ggatgatatc
     3841 gccaatacga aactggtcgt tatgttcgga aataacccgg cagaaacgcg gatgagcggc
     3901 ggtggtgtca cttactacgt cgagcaagcc ctcgaacgtt caaacgcacg catgatcgtc
     3961 atcgatccac gctataacga cacagctgca gggcgtgaag atgaatggct gcccattcgc
     4021 cctggcaccg atggcgcact ggcctgtgcg attgcctggg tactgattac tgaaaacatg
     4081 gtcgatcagc catttctcga caaatattgt gttggttacg atgaaaaaac gctgcccgcc
     4141 aacgcaccac gtaacgcgca ttataaagcc tatattctgg gcgaagggcc tgacggcata
     4201 gctaaaacgc cggaatgggc agcaaaaatc accagcatcc cggcagaaaa aattatccag
     4261 ttggcacgag agatcggttc agcaaaacct gcttatattt gtcagggttg ggggccacaa
     4321 cgacactcca acggcgaaca aacgtcccgc gctattgcca tgctttccgt tcttaccggt
     4381 aacgtcggca taaacggcgg caactcaggt gtacgcaaag gtagctggga tctgggggta
     4441 gaatggttcc cgatgctcga gaatcctgtt aaaacgcaga tttccgtttt tacatggaca
     4501 gatgccatcg accatggtac gaaaatgacc gcgacccgtg atggtgttcg tggaaaagaa
     4561 aaactggatg tccccatcaa gtttttatgg tgctacgcca gtaacacatt gatcaatcaa
     4621 catggcgaca tcaatcacac ccatgaggtg cttcaggatg acagcaagtg cgagatgatt
     4681 gttggcattg accacttcat gacggcctcg gctaagtatt gcgatatcct gttgcctgac
     4741 ctgatgccaa cagagcaaga agaccttatc tctcatgaat ctgcagggaa tatgggctat
     4801 gtgatcctcg cccaacccgc aacctcagca aaatttgaac gcaaacccat ctactggatg
     4861 ctgagtgaag tcgccaaacg cttaggacca gacgtttatc aaacctttac tgaaggtcgc
     4921 agtcagcatg aatggatcaa atatctccat gcgaaaacga aggaacgtaa ccctgagatg
     4981 cccgactacg aggagatgaa aacgaccggg atctttaaga aaaaatgccc ggaagaacac
     5041 tacgtcgctt tccgcgcatt ccgtgaagat ccacaggcaa acccgttgaa aacaccttcg
     5101 gggaaaatcg aaatttattc tgaacgactg gcgaagattg cagatacctg ggaattgaaa
     5161 aaagatgaaa ttattcatcc cattcctgcg tatacctcag gttttgatgg ctgggacgct
     5221 cccctgcgga aaacctatcc actgcagtta acgggcttcc actacaaagc gcgtacccac
     5281 tccagctacg gcaatattga tgtgttacag caggcctgcc cacaagaggt gtggatcaac
     5341 cccattgatg ctcaggcacg cggtatccgt catggcgata ccgtgcgggt atttaacaat
     5401 aatggagaaa tgctgattgc cgcaaaagtg actccgcgta ttctgcctgg cgttaccgcc
     5461 atcgggcaag gtgcgtggct taaagcggat atgtttggtg accgggtcga tcacggcggc
     5521 agtatcaata ttctgacctc tcaccgccct tcaccgctag caaagggaaa cccgtcgcac
     5581 agcaatcttg tccagatcga aaaggtttaa ggagtagccc atgaccacac aatatggatt
     5641 ttttatcgat tccagccgct gtactggctg taaaacctgc gaactggcgt gcaaagattt
     5701 taaagatctt ggcccggaag tcagtttccg ccgtatttat gaatatgctg gcggcgactg
     5761 gcaggaagat aatggcatct ggcaccagaa cgtgttcgct tattatctct ccatctcctg
     5821 taaccattgc gatgaccccg catgtacaaa agtctgcccc agcggcgcaa tgcataagcg
     5881 tgaagatggc tttgtggtgg ttgat
//
LOCUS       DADLTF010000197         5852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.199, whole genome shotgun sequence.
ACCESSION   DADLTF010000197 DADLTF010000000
VERSION     DADLTF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.199"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(618..923)
                     /locus_tag="JN749_003798"
     CDS             complement(618..923)
                     /locus_tag="JN749_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370036.1"
                     /translation="MGSKIAGVVFLYFAVWGMASVIELQYWRNADTYPVWLTKHLRIS
                     PENNDKSLIRIEMETFDKVCGDKRGLLAVTQKQNGMFMRCDDSPGFFSWFSGVYKVN"
     gene            complement(977..1228)
                     /locus_tag="JN749_003799"
     CDS             complement(977..1228)
                     /locus_tag="JN749_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004182043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAY7370037.1"
                     /translation="MTNIILCDVTASVSELKNDPVATASARGGYPVEIIDRNRPVFYC
                     VPAALYEQMLDELDEKDLVQTITERQNQPLREVDLNQYL"
     gene            complement(1307..2578)
                     /locus_tag="JN749_003800"
     CDS             complement(1307..2578)
                     /locus_tag="JN749_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAY7370038.1"
                     /translation="MFALADVNSFYASCEKVFRPDLRGKPVVVLSNNDGCVIARSAEA
                     KRLGIKMGTPWFQLKEAQFPEKLYVFSSNYELYASLSNRVVALLEELSPRVEQYSIDE
                     CFLDARGISHCMDLEDFGRQLRGHVLSGTGLTIGVGFGATKTLAKSAQWASKEWPQFS
                     GVLALSPENPRRTAKLLSLQPVEEIWGVGNRIAKKLHVMGITTALQLSLTNPTFIRKN
                     FNVVLERTVRELNGESCISLEEAPPPKQQIVCSRSFGQRITTYGEMRQAVCQYAERAA
                     EKLRGERQYCRHISTFIKTSPFAVNEPYYGNVATEKLNTPTRDTRDIIAAAVRSLDRI
                     WLDGHRYAKAGIMLNDFSPNGVAQLNLFDDVQPRPHSDALMKVLDGINHSGLGKVWFA
                     GRGIAPDWQMKREMLSPAYTTRWKELPVARF"
     gene            complement(2578..3003)
                     /gene="umuD"
                     /locus_tag="JN749_003801"
     CDS             complement(2578..3003)
                     /gene="umuD"
                     /locus_tag="JN749_003801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HAY7370039.1"
                     /translation="MKFIPAVSEASIVKIPLFTERCPAGFPSPAANYTESELDLNEYC
                     IHRRHSTYFVRAIGNSMTDIGLYSGDLMVVDKAEQPRHGDIVIAEIEGEFTVKRLLLT
                     PRPALQAMNPDFPSLYPDPETLQIFGVVTAFIHKTRRAD"
     gene            3104..4324
                     /locus_tag="JN749_003802"
                     /pseudo
     CDS             3104..4324
                     /locus_tag="JN749_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
     gene            4779..5744
                     /locus_tag="JN749_003803"
     CDS             4779..5744
                     /locus_tag="JN749_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HAY7370040.1"
                     /translation="MTMNLHTQTTAPKASQATSVSPLEQSGSSKTKFSRGKSKARAGA
                     VKTDLYQTVTDSIIAALESGVKPWVCPWVRNGAAVGLPANFSTGTAYSGINIMLLWCS
                     AAKQGFQDERWLTYKQAQELGGQVRKGEHGTTAIFYKTLEKEDEDGEIEKIPMLKAFT
                     VFNVEQIDGLSIESVPQPVAGFDPLPQAEALMIRSGAKITEQGVKAFYRPATDEIVLP
                     ERFRFADAANFYATGLHELVHWTGAASRLNREKGNKFGSEAYAFEELIAELGSAFLLA
                     DLGITGEVQHESYIASWLKALKGDKRYIFKAAAAASKAHRWLMDC"
BASE COUNT         1381 a         1557 c         1598 g         1316 t
ORIGIN      
        1 ctgtcagctt atctgcctgg gaccacggtc ccacctgtat cgccggtcag gttatctgtc
       61 tgggaccacg gtgccacctg tatcgccggt caggttatct gcctgggacc acggtcccac
      121 ctgcatcgtc ggcacattat tagttaggga caacggttca atcaatgcct gtcacgaaca
      181 ggtattggct tgtcgccagc aacccgttga gcggagtaat cacgcagcag gacggctgtt
      241 acaggcgatg gtgaaagctc tgttgggatc tgaacgaagc atgttgagtg aaatttgcaa
      301 aatttcgcag agtatgcggc ttcatcttta aatttccgcc agcattttta aagccagcta
      361 ccgacgctgc gcgttggccg gcccttcgtc cggcgggtaa ccagtaccgg ccgatcttac
      421 gaccctggat gaacatcatc ccgtcagcag cgtctccact gtgatgtcgt ttttcacact
      481 gtccgatcac aaaatagaac gggcagggac aacatatggt aggaaaagca gctgccagag
      541 acacaatata ttgattaccc acagtaacgg gggataacca tgtgggtaac atattgaaag
      601 tgcaacacgt tatctgatta attgactttg taaacgccgc tgaaccatga aaaaaaaccg
      661 ggggaatcat cacagcgcat aaacataccg ttttgttttt gggtgacggc aaggaggccc
      721 cttttatcac cacatacctt gtcaaaggtt tccatttcta tccttatcag gctcttgtcg
      781 ttattttcag gagagatccg gagatgtttt gtcagccaga caggataggt atcagcgtta
      841 cgccagtact gcaattcaat cactgaagcc attccccaga cagcgaagta taagaaaacc
      901 acacctgcga ttttgctacc catattgttc cttaacattg gcttaataca ttccttgctc
      961 ctcgcaccgg agcacatcac agatactgat tgagatccac ttcacgcagc ggctggtttt
     1021 gacgttctgt tatcgtttgc accaggtctt tttcgtccag ctcatcgagc atttgttcat
     1081 acaaagccgc gggcacacaa taaaaaacag ggcggttacg gtcaattatc tcgaccgggt
     1141 agccgccgcg ggcactggcg gtggccaccg gatcgttttt aagttcgctg acgctggcag
     1201 tcacatcaca cagaatgata tttgtcatgg ttcccccctg agacaagtca cctgtatgat
     1261 cggtaaagta agctctggcg gcaagtccct tctgtcattt caacggtcag aaacgggcaa
     1321 ccggcagctc cttccagcgt gtggtatatg ccggtgacag catctcccgt ttcatctgcc
     1381 agtccggcgc aataccccgt ccggcaaacc agactttccc gagtccggaa tgattaatgc
     1441 cgtcgagtac cttcatcagc gcatcgctgt gcggccgtgg ctgcacgtca tcgaacagat
     1501 tcagctgcgc gacgccgttc ggactgaaat cattgagcat tatccccgct tttgcgtagc
     1561 ggtggccatc gagccatatc ctgtccagag acctgaccgc tgccgcgata atgtcccggg
     1621 tgtcacgggt aggggtgttc agtttttccg tggccacgtt accgtaatac ggctcattaa
     1681 ccgcaaaagg ggatgtcttg atgaaggtgc tgatatgccg gcagtactgt cgctcaccgc
     1741 gcaacttttc cgccgcgcgc tcggcatact ggcagacggc ctggcgcatt tctccgtagg
     1801 tggtgatccg ctgcccgaag ctgcgactgc agacaatctg ctgtttcggc ggcggcgctt
     1861 cctccagcga aatgcaactc tcgccgttca gctcacgtac cgtgcgctcc agcactacgt
     1921 tgaagttttt ccggataaat gtagggttcg tcagcgacag ctgcagggcg gtggtgatcc
     1981 ccataacgtg cagctttttc gctatccggt tccccacgcc ccagatttct tccaccggct
     2041 gcaggctgag taattttgcc gttctgcgtg gattttcggg cgacagcgcc agcaccccac
     2101 tgaattgtgg ccattccttt gacgcccact gcgccgattt ggccagtgtt tttgtggcgc
     2161 cgaagccgac accgatcgtc agtccggttc cactgagaac atgcccacgc agctgcctgc
     2221 caaaatcctc cagatccata cagtggctaa ttccccgggc atccaggaaa cattcgtcaa
     2281 ttgagtattg ttcgacgcgg ggcgacagct cttccagcag ggcgaccaca cggttactca
     2341 ggctcgcgta gagctcgtag ttgctggaaa aaacgtacag tttttcagga aactgcgcct
     2401 ctttgagctg gaaccagggg gttcccatct ttatacccag ccgtttggct tcagcactcc
     2461 gggcaataac gcagccatcg ttgttactga gaacgacgac aggtttgccg cgcaaatccg
     2521 gcctgaaaac cttctcgcaa ctggcgtaga aggagttgac gtctgccagg gcgaacatca
     2581 gtctgccctc cgggttttat gaatgaaggc cgtcaccacg ccgaaaattt gcagagtttc
     2641 cggatccggg tacagggaag ggaagtccgg gttcatcgcc tgcagggctg gccgcggcgt
     2701 cagaagcagg cgttttaccg taaattcacc ctcaatttcc gcgataacaa tatccccgtg
     2761 ccgcggctgt tctgctttat ccacgaccat cagatcgccg gaatagaggc caatatcagt
     2821 cattgagttg ccaatcgctc tgacaaaata ggtggagtgg cgccggtgaa tgcagtactc
     2881 attcagatcc agttcagact cagtatagtt tgctgccggt gacggaaaac ccgcagggca
     2941 tcgctctgtg aacaaaggaa tttttactat gctggcttct gatactgcag gaatgaactt
     3001 catcgttttt tccctcgctt aaaactgtat atgcatgggt cttcccttgt tgtggtggct
     3061 gaaggcatga taatggtgta tttaatcgcc agaggtcacc gccatggacg aaaagtccct
     3121 ctacgctcat attctcaacc tgtccgatcc gtggcaggta aagtcccttt ctctcgatga
     3181 aaatgccggt tctgttactg tcactattga gatcgctgaa aacacccggc tagcctgtcc
     3241 gacctgcggt aaatcctgtt ctgttcacga tcaccgtcat cgtaaatggc gccatcttga
     3301 tacctgccag ttcaccacta ttgttgaagc cgatgttcca cgaattatgt gtccggagca
     3361 tggctgcctg acgttgcctg ttccgtgggc tggccccgga agccggtata cgttgctatt
     3421 cgaatcgttc gttctctcat ggctgaaaat cagcaccgtt gatgctgtca ggaagcaact
     3481 taagctcagt tggaatgcgg ttgacggcat tatgacccgg gcagttaagc gaggtcttgc
     3541 ccggataaaa aagccattat ccgcccgtca tatgaatgtg gatgaggtcg cctttaaaaa
     3601 aggacatcgt tacataacgg tgatctccga tcgcgatggt cgggcgctgg ccttaacgga
     3661 tgatcgcggc acagagagtc ttgccggcta tcttcgcacg ctcactgatg ggcagttgct
     3721 ggctatcaaa acgctctcaa tggacatgaa cgcgggctat ataagagcag cgcgtatcca
     3781 cttacccagt gcggttgaga aaatcgcctt tgaccgcttc catgtggcga agcaactggg
     3841 cgaggtagtt gataaaaccc gtcagaatga acatccgcac ctccctgttg aaagccgaca
     3901 ccaggcaaaa ggaacccgct tcctgtggca gtacagcgat aagtggatga ccgaatcccg
     3961 gcaggaaaag ctgatgtggc tgcgtgcaca gatgaagctg acgagccagt gctgggcgct
     4021 gaaagagctg gcaaaggata tctggaacag gccatggagc gaggaaagac ggagtgactg
     4081 gcagagatgg ttggcgctgg cggctaacag tgacgttccc atgatgaaaa atgccgcgaa
     4141 aacgatagga aaaaggctgt aggggatcct gaatgcgatg cgacacagtg tctcaaacgg
     4201 aaatgcggag gcacttaaca gcaagatcag gctgctgagg ataaaagcca ggggataccg
     4261 aaaccgggag cgctttaaac tgggggtgat gttccactac ggaaagctga atatggcgtt
     4321 ctgagccttc ccaccatgat cggggaagac ccatatgcat acagtataag gggcggtgaa
     4381 atttctgcaa gtgatggggg gatagccggt cagaacgtcc ccgaaaacaa accgcccccg
     4441 gtttcaggaa gggcgaactg accggcagag gcagtgtaac accgcatgtc acaaaggcag
     4501 cactgacgtc gtcagtgctt tttttacccg tcctcccgga cgctgaaggg cagtttcacc
     4561 cgtgcagcta aaggggcggt aagtccgggt gtgcacatcg tcattttctc ctgtgatgcc
     4621 tttctcctgc gtatcagctt ccggtaatcg tcccccgtca caacctggac agcaagggta
     4681 aatggcacgc tgcgcgccct tgctgaccag aacgactccg ggcaggccga accgtcaggc
     4741 gatacgaaga cgaaattcac actgacggag aaacagccat gacgatgaac ctgcacactc
     4801 agacaaccgc ccctaaagct tcacaggcga ccagcgtatc cccgctggag cagtcaggct
     4861 cctcaaaaac gaaattttcc aggggcaaaa gcaaagcccg tgctggcgca gtcaaaacag
     4921 acctttacca gacagtaacg gacagcatca ttgcagccct ggaaagcggc gttaagccgt
     4981 gggtgtgtcc gtgggttcgc aacggcgcag cggtgggatt accggccaac ttcagcaccg
     5041 gcacggcata cagcggaatt aatatcatgc tgctgtggtg tagtgcggca aaacagggtt
     5101 ttcaggacga gcgctggctg acctacaaac aggcgcagga gctgggcggc caggttcgta
     5161 aaggtgaaca cggcacgacg gccatctttt acaagacgct ggagaaagag gacgaggacg
     5221 gggaaatcga aaaaatcccg atgctgaaag cctttaccgt gtttaacgtg gagcaaatcg
     5281 acggcctgag catcgagagc gtgccgcagc cggttgccgg tttcgatccg ctgccgcagg
     5341 cggaagcact catgatccgg agcggggcaa aaatcaccga gcagggcgta aaggcgttct
     5401 atcgtccggc caccgatgaa atcgtcctgc cggagcggtt ccgcttcgcg gacgcggcga
     5461 acttctacgc caccggcctg catgagctgg ttcactggac gggtgcagcg tcccgcctca
     5521 atcgtgaaaa aggcaataag tttggctctg aggcgtatgc gtttgaggaa ctgatcgccg
     5581 agctgggcag cgcgtttctg ttggcggatt tggggattac gggagaggtg cagcatgaaa
     5641 gctatatcgc gtcctggctg aaagcgctga agggagacaa gcgctacatc tttaaagcgg
     5701 cggcagcggc ctcaaaagcg caccgctggc tgatggactg ctgacagctg gagccctgcg
     5761 gtgcagggct tcagaatttt tgaaagagag agaatcaggg agggaattat ataagtcact
     5821 gttttttaga attctgtaac gcggccggcc tc
//
LOCUS       DADLTF010000198         5786 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.200, whole genome shotgun sequence.
ACCESSION   DADLTF010000198 DADLTF010000000
VERSION     DADLTF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.200"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..789)
                     /locus_tag="JN749_003804"
     CDS             complement(<1..789)
                     /locus_tag="JN749_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M15"
                     /protein_id="HAY7370041.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLG"
     gene            1035..1661
                     /locus_tag="JN749_003805"
                     /pseudo
     CDS             1035..1661
                     /locus_tag="JN749_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            complement(1658..1864)
                     /locus_tag="JN749_003806"
     CDS             complement(1658..1864)
                     /locus_tag="JN749_003806"
                     /inference="COORDINATES: protein motif:HMM:NF019607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7370042.1"
                     /translation="MAAKIIYWALKDKDVIVMSVNGDKVTEDGRILTDRGQRIRDVRT
                     GPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(1834..2772)
                     /locus_tag="JN749_003807"
     CDS             complement(1834..2772)
                     /locus_tag="JN749_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HAY7370043.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARGEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGR"
     gene            complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="JN749_003808"
     CDS             complement(2883..3266)
                     /gene="bssR"
                     /locus_tag="JN749_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HAY7370044.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3479..4804
                     /gene="rimO"
                     /locus_tag="JN749_003809"
     CDS             3479..4804
                     /gene="rimO"
                     /locus_tag="JN749_003809"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HAY7370045.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4851..>5786)
                     /locus_tag="JN749_003810"
     CDS             complement(4851..>5786)
                     /locus_tag="JN749_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HAY7370046.1"
                     /translation="FISFDGKTFVYYLYPVTHNRSLIFLLGLERFSLLSKSLAMDSEN
                     LMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVFAYKKDVYLRVCTLII
                     FFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSM
                     YNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMK
                     VVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIG
                     VAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         1482 a         1488 c         1372 g         1444 t
ORIGIN      
        1 ccccagcact acggagatta aacgcatatc accctgggta gccgaagcaa ccagattata
       61 tcccgcacct gccgtggttc ctgtcttcat gccatcaaca ttcagattgc tgctccacag
      121 cagacggtta cggttaggct gacgaatttt gttgaaggtg aactctttct ctttatgaat
      181 ggcgtactct tccggcacat cgtggatcaa tgctttaccc agcaatgcca tatcgcgcgc
      241 ggtactgaac tgccccggcg catccaggcc gtgcaccgtc tggaaggtag tgttggtcag
      301 ccccagtttt ttggcataac cattcatcag accaataaag gactcctggc tcccggcaac
      361 gtaatcggcc agcgcaatac aggcgtcatt accggactgg ataatcacac ctttgttcaa
      421 gtctgccacc gaaacctgat cgcccggttt gaggaacatt accgatgaac cacgcagtgc
      481 cggatttccc gtcgcccagg catctttacc gaccgtcacc atatcggtga gtttaatctt
      541 atcggcctta agcacctgcc caaccacata gctggtcatg attttagtca ggctcgcggg
      601 atccagtttc tcatccgcat tgccttctgc cagcacttta ccgctggcgt aatccattaa
      661 aatccatgca cgcgcatcca cgctcggcgc ttcaacggtt tgttcggccg cgaatgccgt
      721 tggggcaaaa aggaataaaa atgcagaacc cgctgcaaga gcacgaagga gagaggagta
      781 ttgcgtcata aatgccaccc gagtatccat tcaaaaaaat acgttatatc gccgtatgat
      841 aagttcggtg attaataacg cacacacaaa cttaaagaaa ccgtaagttg gtaaagtttt
      901 taaagtttat gcaatatcct gccgcttcgc tgcttctggt gtcatttttt tgccagctgt
      961 tgcttaaaag ttaactttac ttcaccatcg gagtcctgtt cagtctgtgg ctgcctgtcg
     1021 gaaaaggggt gagtatgatt acgctgtggg gtcggaataa ttcaactaac gtaaaaaaag
     1081 tattgctgac gctcgaataa ctggaactac cttatgagca aattctcgcg ggccgtgagt
     1141 ttgggataaa tcacgatgct gattttctgg cgatgaaccc taacgggctg gtgccgttgt
     1201 tgcgtgacga cgaaagtgat ctcattcttt gggaatcaaa cgccattgtc cgctatctgg
     1261 cggcacagta cgggcaaaaa cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg
     1321 aaaaatggat ggactgggca aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg
     1381 gattagtcag aacaccaccg gaagagcgcg atcaggccgc cattgatgcc agttgcaaag
     1441 agtgcgacgc cctgtttgcc ctgctcgatg cggaactggc aaaagtaaaa tggttctctg
     1501 gcgacgagtt tggtgtgggc gatatcgcta tcgcaccgtt catctacaat ttgtttaacg
     1561 ttggcctgac ctggacaccg cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac
     1621 gccccgcagt gcgcaaagtg gtgatgattc ccgttagcta attgcgtggg ctaactttaa
     1681 gtaattcccc actggactcg tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa
     1741 catcacgaat tcgctgccct ctgtccgtta aaatacggcc atcttctgtc actttgtcgc
     1801 cgttgacgct catcacaatg acatctttat ctttcagcgc ccaataaata atttttgctg
     1861 ccactggggg aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt
     1921 ccagtaaaaa acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt
     1981 aaagcctgaa tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg
     2041 cgggatatta atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact
     2101 ccacggattc atcgccattc cttgcggatt acgaatgcca taagaccaga tctcgccgcg
     2161 cgcaccggat tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg
     2221 caccagtttg ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc
     2281 cagagcaata aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc
     2341 ggtagacagt tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc
     2401 atcacttaag cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga
     2461 ataacttaac cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag
     2521 gccgccctgc ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt
     2581 tcctgcttgc cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc
     2641 gggtaaaaag gccagtgccc agggatggtc gagtttgtct tgcagtactt cgacatttac
     2701 cgttgcagga gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga
     2761 ttgtcgatgc ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat
     2821 aacaaattca tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt
     2881 tttcaggcct tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc
     2941 atcagacaat tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc
     3001 ccactcatca aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc
     3061 cactcttcct gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc
     3121 agtttaaaaa agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc
     3181 ttcctcagtt gcacgtatgc agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga
     3241 tcgattcgct gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat
     3301 taaatagtat ggctattttt tgagcaaaat gctgtctagg atcaattctt cacgccgaat
     3361 taacaaaatt tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat
     3421 ccagaactct gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat
     3481 gagcaaagta actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct
     3541 tgtcgattca gagcgtattc tcaccgaact ccgcactgaa ggttatgacg tggtaccgag
     3601 ctatgacgat gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca
     3661 agaatcactg gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg
     3721 ttgtctgggg gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac
     3781 cgggcctcat agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa
     3841 acacaaccca ttcctgagcc tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta
     3901 tgcctatctg aaaatttcgg aaggctgtaa tcaccgctgc accttctgca ttattccgtc
     3961 tatgcgcggc gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct
     4021 ggtagatgcg ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt
     4081 tgatgttaaa catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag
     4141 cctgtgcgaa cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta
     4201 tccgcatgtg gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga
     4261 cattccgttg cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt
     4321 agatcgccaa ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg
     4381 ctcaaccttt attgtcggct tccctggcga gacggaagaa gatttccaga tgctactcga
     4441 cttcctgaaa gaagcacgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg
     4501 tgcagacgcc aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa
     4561 ccgtttcatg cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg
     4621 tgaaattctg gtgattatcg acgaagtgga cgaagaaggc gcgattggtc gcagcatggc
     4681 agatgcaccg gaaatcgacg gcgcggttta tctcaatggt gaaaccaaca ttaagccggg
     4741 tgatatcctg cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt
     4801 ttaattaatt caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag
     4861 ctaatctgtg ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc
     4921 aagcgttcga cggtagtaca tttatttgaa tgcgctacac cgatactggt gcttaaatta
     4981 atgcttccca cctggaaatg aacgatttct tttagtatcg atagcaactc ttccagcgtg
     5041 ccgccagtat aaatcaccgc aaattcgtca cctccgaagc ggtagacttt acctttatct
     5101 accgggatct gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa
     5161 tgaccgaagg tgtcgttaat gtttttgaat ttatcgacgt caaacagaca caaataaccg
     5221 cctttttccg gcagcgcttt taaatcttca tcaaaggatt ttctgcccat tgctttggtc
     5281 aggtcgtcca ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa
     5341 aagaaaagcc cgccatccag tacgcgttca aaatggttat taatgatggc ttctttctcc
     5401 acaataccac gattaatcac tctgcgtacc agatagaggc aactggcacc cgatatcact
     5461 gccaccaatg cggcaaagaa aataatcaat gtacaaaccc gcaaataaac atctttttta
     5521 tacgcaaata cgtacaatcc ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg
     5581 gtgaagatgg cgtttttagc attatattca tctccggtca ccggtttacc gttcttaaat
     5641 agagagaaca tcaggttctc gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc
     5701 tccagaccaa gcaaaaatat cagactacga ttgtgagtta ccggatagag gtaatagacg
     5761 aaggttttac cgtcaaaaga gataaa
//
LOCUS       DADLTF010000199         5781 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.201, whole genome shotgun sequence.
ACCESSION   DADLTF010000199 DADLTF010000000
VERSION     DADLTF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.201"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..848
                     /locus_tag="JN749_003811"
     CDS             <1..848
                     /locus_tag="JN749_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370047.1"
                     /translation="QEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAER
                     QIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEA
                     DRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQ
                     RALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAA
                     GDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPV
                     AENVE"
     gene            1087..1146
                     /locus_tag="JN749_003812"
     CDS             1087..1146
                     /locus_tag="JN749_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HAY7370048.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1463..1522
                     /gene="ibsE"
                     /locus_tag="JN749_003813"
     CDS             1463..1522
                     /gene="ibsE"
                     /locus_tag="JN749_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HAY7370049.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1642..3075)
                     /gene="hldE"
                     /locus_tag="JN749_003814"
     CDS             complement(1642..3075)
                     /gene="hldE"
                     /locus_tag="JN749_003814"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HAY7370050.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3123..>5781)
                     /gene="glnE"
                     /locus_tag="JN749_003815"
     CDS             complement(3123..>5781)
                     /gene="glnE"
                     /locus_tag="JN749_003815"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HAY7370051.1"
                     /translation="QADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIAWAQ
                     TLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGMGKL
                     GGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYR
                     VDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAML
                     RPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQLIR
                     GGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTL
                     PSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSEQWR
                     ELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLMPHL
                     LSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIASQL
                     ARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLR
                     IAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRGFAV
                     VGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFSTRT
                     SSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQLTA
                     HFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIEFIT
                     QYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQ
                     ELPGHVSEDCFTAERDLVRASWQKWLVEE"
BASE COUNT         1369 a         1673 c         1467 g         1272 t
ORIGIN      
        1 aacaagaagc gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac
       61 agaatgcacg tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa
      121 ttctggctga acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa
      181 gaaaggttga ggctgaacgt gaagttcgta ttaaagagat cgaacagcag caggtcaccg
      241 aaatcgccaa ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg
      301 cagaagccga tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca
      361 aagcggttga actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg
      421 cggctatcgt tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac
      481 aacgtgcgct gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca
      541 aactggcctt gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat
      601 ctattgacgg tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg
      661 atgcaaacac gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc
      721 tctcttaccg cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag
      781 gcggttcact ggcggcattg acttcaccct taacctcaac aactcccgtc gccgaaaacg
      841 tagaataaat tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct
      901 gcgttccgct aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg
      961 gaaggcaaaa ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg
     1021 gagtcagcaa agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa
     1081 agggttatga tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct
     1141 tattaacagc caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg
     1201 ttgaaaaaaa atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg
     1261 gggatttttt atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt
     1321 tgcgtgaccg ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg
     1381 ctcatcaaca atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg
     1441 ggccttatag aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact
     1501 gttaagtttc ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt
     1561 gttcctttac tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga
     1621 gatctgtgaa ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt
     1681 agtggtcgag caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac
     1741 ttctttactc ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg
     1801 caggatcccg gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac
     1861 cgcttccagt gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc
     1921 ccctttcagc cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag
     1981 tttgcgggca tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt
     2041 ggtcatcacc actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc
     2101 ttcggtcatc acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat
     2161 cggcgaaacc gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa
     2221 gaagcaggct tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac
     2281 cgtgtcgccc gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg
     2341 cgctttaccc ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga
     2401 gagttcgtaa tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca
     2461 tttaccgaca acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta
     2521 gcgctcaaaa tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag
     2581 ttggatcatc tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc
     2641 gccaatcgaa ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc
     2701 gaaaccttct tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt
     2761 ggtgatggtc ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag
     2821 agatttactc agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt
     2881 agcaccgaga gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat
     2941 ggtattcact ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta
     3001 ccagtaacga tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg
     3061 cagcgttact ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata
     3121 cttcattctt ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg
     3181 aagcaatcct ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg
     3241 cgcagcgtcg tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt
     3301 tgcgccagca gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca
     3361 tgagcgtagc gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca
     3421 tcggctttga tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg
     3481 cgcatttccc gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca
     3541 cgacgcaccg cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc
     3601 accagcgcct gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct
     3661 gcggatgtca ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac
     3721 aaaatgccgg aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga
     3781 taaaactgcc gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca
     3841 tggaggaaga taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg
     3901 tagccgacca ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag
     3961 cgggccacca tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc
     4021 caggttaagt gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca
     4081 atgcgtaaca gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca
     4141 tcttccggca cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc
     4201 ggctggtaaa gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc
     4261 tggctggcaa tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg
     4321 aattcactga gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta
     4381 atgcgcgaca gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc
     4441 atcagatggt cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg
     4501 cggaaatcag caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc
     4561 actggcgtag tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac
     4621 agcgactctt cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc
     4681 acattggtca tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc
     4741 gcccacgcca gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg
     4801 ttaatgcttt gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt
     4861 tcagcatcgt tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa
     4921 gagcgcgact gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata
     4981 aattcaattt cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga
     5041 cgcacttcac gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg
     5101 atgtaacgac ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct
     5161 tcgctatcgc ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc
     5221 tcctggtaat aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac
     5281 ggacgcagtc gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact
     5341 ttaatcagcc gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg
     5401 ccctgcgtac aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattc
     5461 agttccccgc cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc
     5521 gcattgcacg gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca
     5581 acaatcagcg tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt
     5641 gccagcgttt gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg
     5701 cgcattaacc cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa
     5761 tgttgccatt cgtcagcctg t
//
LOCUS       DADLTF010000200         5746 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.202, whole genome shotgun sequence.
ACCESSION   DADLTF010000200 DADLTF010000000
VERSION     DADLTF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.202"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1472)
                     /locus_tag="JN749_003816"
     CDS             complement(<1..1472)
                     /locus_tag="JN749_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7370052.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEDEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNRTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLT"
     gene            complement(1690..2562)
                     /locus_tag="JN749_003817"
     CDS             complement(1690..2562)
                     /locus_tag="JN749_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HAY7370053.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHWGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDGLRTESVRGQAREQFTGAVDRIFDTAESVCVASVARKVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            2893..3075
                     /locus_tag="JN749_003818"
     CDS             2893..3075
                     /locus_tag="JN749_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370054.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            complement(3304..3378)
                     /locus_tag="JN749_003819"
                     /pseudo
     CDS             complement(3304..3378)
                     /locus_tag="JN749_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3375..3800)
                     /locus_tag="JN749_003820"
     CDS             complement(3375..3800)
                     /locus_tag="JN749_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HAY7370055.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPGEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMMLENPSPELLTVLIRELSGRGR"
     gene            complement(4918..5430)
                     /gene="clbS"
                     /locus_tag="JN749_003821"
     CDS             complement(4918..5430)
                     /gene="clbS"
                     /locus_tag="JN749_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin self-protection protein ClbS"
                     /protein_id="HAY7370056.1"
                     /translation="MAVPSSKEELIKAINSNFSLLNKKLESITPQLAFEPLLEGHAKG
                     TTISVANLVSYLIGWGELVLHWHDQEAKGKTIIFPEEGFKWNELGRLAQKFYRDYEDI
                     TEYEVLLARLKENKQQLVALIERFSNDKLYGKPWYNKWTRGRMIQFNTASPYKNASGR
                     LNKLQKCLAE"
     gene            complement(5465..>5746)
                     /gene="clbQ"
                     /locus_tag="JN749_003822"
     CDS             complement(5465..>5746)
                     /gene="clbQ"
                     /locus_tag="JN749_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colibactin biosynthesis thioesterase ClbQ"
                     /protein_id="HAY7370057.1"
                     /translation="MTIFTPIIKNDYRLYEQYVFQAKARTLTCPIVLFHGDADNLVMQ
                     DELLAWEKFTTRKTRTIIFPAADHFFVDKHFEQVVGYVNQTIESLEIVG"
BASE COUNT         1482 a         1408 c         1312 g         1544 t
ORIGIN      
        1 gtcagtaatt cccgggttct ggcatcgatc tcctccatgg ccattctgac atcagcggtc
       61 acctgctcct gacagtccgc gatttcccgg atatccgcaa gcaggctggt gatatgcgac
      121 tgcagtgact gaatgctttg ctgtagcccc tgatggcgta atgacagata ttcgctgaca
      181 ttttccgcat tctgcatcag ttttgcggcg gcagaatcaa ccgccagtgc cgccgctctg
      241 gaatgggagc tctgtatgac ttccgttatc agttgatcga taagtgagcc ttcccacagg
      301 tctgtcgccc cttcggttgc catttcactg tctttacaga cgtattcttt tttcatgcga
      361 ccgaaggctt ccctgacaaa atcatcgacc caggcttcat caacaggaag ggttctgttt
      421 ttccgtaact catgaagcgc acggttagcc aggtacgcct gacgtgatga gcctggataa
      481 acgcgggagg cgggcagcac atcgctgttc agcattgccg gaactttctg gcgtacggca
      541 tctgccccat cgccattgcg gtctttctca tcaaatttat tgaccagcac aaacaggcgg
      601 ccggctgata cgtcagcaat ggcattaagc tctttacgga cgtcttcatc cgcttttgag
      661 ttcatctggg tgtaatccat caccgccagc accgcagagg ctttctgcag ttgatcccgc
      721 atcatcactt ccatctgcgg ttgtccggcc tcattagggc cgggggtatc cagcagtgtg
      781 agtgttccct ggcatgcatc catccccacc agatggctga attccacgtc gatcaccggc
      841 agtttctgca cttctgcgta ttcatcaaaa ggaaactcac tccccattgc cgcagcaagg
      901 cgaaccagat cgtttaacga tgccagtccg gtaaagattt cgtcttcccc gtggtattca
      961 tttttgagcc agccatcatt tgccagaata tccagcagtt cgcaggtatc cccggtttgc
     1021 tgcagggtct gtgcgacttg ctgtcctgcc ggggtgtcga gtttttgttg cagtgtgctt
     1081 aataaattgc ggacgggctg aatatgttcc agatggagga ccggctcagt ttttccggga
     1141 acgtggcgga tgagcgtcgg tacagaggtc atggggcggt tacggttagg cagaatttcc
     1201 tgccccacaa tggcgttgat ggtagttgat ttacctgctt tcatcgtgcc gacaacagcc
     1261 agcaccatct ccctgcgggc ggttttgatt tgttcgccct ccagctcctc aattcttttc
     1321 agtgctttct gtttatcaaa aagcagtctg ttttcgttct tactgtcaga taacacatcc
     1381 ggttcatcaa ccatttgccg gagcagatta atgttcagtt gcaaaagtcg gtcggcttca
     1441 tcacaaagca gggcgatgtt cttttcgtgc attatttaac cttcctgaga cgcattttca
     1501 tgacaaccac ggaggaggtc atttaccaca aacgatcgtt tatagcgatc gatatgatag
     1561 taacgatcga tgcattctat taacttgaga aacacaacgc aaggggacaa cccgggtatg
     1621 gctgtgtttg cattgtgttt tcatggggtt aaaaatctgt aacaagagtc tggtgcatca
     1681 ccaccgggtt cagaagaaaa tccagttcca taccgcgcgg gcaacagaga ccacggtgtc
     1741 acgaacagcg cgcagaacct tacgggcaac agaggcaaca cagacgctct ccgccgtgtc
     1801 aaatatccgg tccaccgcac cggtaaactg ttcacgggcc tgaccccgga cagattccgt
     1861 gcgcagcccg tcctgcaggc gggtcatcag gggactggcg gcatggtcgg gaagcgctgt
     1921 catgagcgca ctgaccagcg tatccagttc ccagccagtg cgggccgata cggccacaac
     1981 cggatgtacg ggccggaaca gacggaatac cgcctccgtt ttttcgcgaa tattctgtgc
     2041 ctgtgcagga gaaggctgaa taccggccat atcccattca tggcagggct ccgttttgtc
     2101 ggcctgcgtc accacaaaca gtacccgctg atgtccccag tgcaggatgt gtcgccagaa
     2161 atactcatcc acagacaggg cacggtcatc ggctttaatc agccacagta ccaggtccag
     2221 ttcaggcaga atgtcacggt acagggcctc atactctgca tccctgtccc ggctctcgcc
     2281 caccccgggc aggtcagtga taaccatgct gtgaccatgg ccactcagac ggaagcgccg
     2341 tacttcccgg gtgccggcgt gaacatcact gaccggggtg acctccccct gaaacagtgc
     2401 attacagagt gaggatttac cggccccgct tttacccata atgccaatca cgggttcgtg
     2461 actggtgagt ttgcgcagat gttccaggat gtgacgggaa agtgagtaag gcagggagga
     2521 gagcggtttt tcaattgcct caatggcatc agacggattc atacatgtat tcccggtgaa
     2581 aaaagacaaa accccgcgca ccggaaaggt ggcgggggac tcacaacgat aatatctgtc
     2641 tgaaattatt taggatacag gtatcttcag ataatcatac ctccagaatt ttgttcttaa
     2701 tagttgagta gtggtatttg gtttccctga gagaatataa aggtgaatca tgctgcactg
     2761 agtttgattc tatttgtact gagacaagta tcttcatgat tataatcaaa agttgggtgg
     2821 gttggtctgg cgctcatcat tttttcgaat aaactcacta attattgtaa ccaccccaaa
     2881 ggagatgatt tcatgtataa ttgtggtgaa tcatattcag aatacgacat atcattatct
     2941 gacgatgaaa actatgacga tgactattat cgtgaacgcc cgtgtccggg ggaagttttc
     3001 ggctatgata atagtgagga taatgatgag gcggcatctg attatgcact aaaaataaat
     3061 gaaagctctg attaatacgg gggaagctga tagtaagcca atcaataaca gatattaatt
     3121 aatgaatgaa tgaatgaatg aataaatata atcaggcgct caatatttta tttaatcttg
     3181 gcgcctgatt aaaatccgag gtaacatata ataattctca tcaaaatagt ggatttgccc
     3241 ctatatttct gagtatctgt tatcacttaa tccattacag atcctccttc agcatatgtc
     3301 gtatccgttg aaagatttac acatatcggt aacaccggca acgagccaga tacgggtacc
     3361 tgatgggagt gaaatcatct tcccctcccg ctcagttcgc ggatcaacac cgtgagcagc
     3421 tctggcgatg gattttccag catcatgtta ccgtgacgga actccacctt gcaggaactg
     3481 gcactgactc tggtctgagt ggaagtggat aaagacggcg caatggccgc cacaggttct
     3541 ttctgctcac ccggcgttat ttctacaggt aataattcaa cgccagtgtc agaagaggtc
     3601 gttaccggaa gacgccgcga aacacgccct tcgttctgcc agagcctgag ccatttgaaa
     3661 ataacattat cattgacgcc attttcacgt gcaatctgtg caacacaagc tccaggttgt
     3721 gatgccagtt ccaccatacg aagtttgaat tcattcgaat agtttttacg aggttctttt
     3781 cgccagtcct gtaattccat acttagatgt ccgtctatat cagatgggcg tctaagttac
     3841 caattctcgt ctgatggcta catacggcgg tcagtttacg cttactacaa tgatgataat
     3901 gcgattatat aaaggtcaaa ccatatggtt gttagcggat ttcaacaacg gtgctgtact
     3961 cgctgagcgg cacatgaagt ttgaataatg tggaaacaac aaatggctac attaagtcat
     4021 tggacatata cagagatggt tggcaaatcg agtacacgat atgttgcctg aaaagtcgac
     4081 cgaacagaac tataaatatc aagtcggcat aacacatcta aatttcataa gggaagatgt
     4141 gccaccgggg acgttttttt caccacgggg acttcgggta taacgatgca atccgaagaa
     4201 agaaatccaa tgttggcgat attcatcatc gctttattcg tccactcatg cgaatggcaa
     4261 taaagcgact caccaaaaca gtttctttag cttccctcta ccaaaattcc ctatgatcgc
     4321 atgttagaac tctgcgtgct gaacttcggg ccatatggac ggtatcattc atgcacttgc
     4381 tcctttgaaa ttgctggata tcattcgtct ttatctgcac cggccggcac aaatagctgt
     4441 aggggaaccc ggcgcaggca aaaagagcga ctttaacgcg ttttgcagga ctccccgttg
     4501 ccttgcaggg agacactgta tcatcaaaca ccatcttaat cccagtacat taccctccat
     4561 cctggctcgt accaaatcag aggaaaggcc tcgcggcaat agattttccc ggcgaagctc
     4621 ggagtgaaat acagtgcagc aatatccgac atcagaattt gatcctcctg cgtcaggata
     4681 cgaaaaaaca tcccgatttg agcacccttg tcgaaaaaaa atcaccttat tatgaagatt
     4741 ggttcttaat acacatgcgt tcgcatgtgc ttatgttaag tagacttatt gtcttacaca
     4801 ttttatccgt atcattaacc agaatatgtt ccggagcagc gggtgatagt gccgccagtc
     4861 cacactcatc gctgcccgga aaaatcatca gtggggaggc aaacggtaag caccccgcta
     4921 ttctgcaaga catttctgca gtttatttaa cctccccgaa gcatttttat aaggcgaggc
     4981 ggtattaaat tgaatcatac gacctcgggt ccatttatta taccaaggtt taccgtaaag
     5041 cttgtcgtta ctgaatcgtt caatcaaagc cacgagttgc tgcttatttt cctttaaccg
     5101 tgccaataaa acttcgtact ctgtaatatc ctcatagtca cggtagaatt tctgtgctaa
     5161 acgccccaat tcattccatt taaatccttc ctcaggaaaa ataatagttt ttccttttgc
     5221 ctcttggtca tgccagtgta acaccagctc tccccagcca atcagatagg aaaccagatt
     5281 cgctacgcta atcgtagtcc ccttcgcgtg cccttccaat agaggttcaa aggcgagttg
     5341 gggcgtaata gattctagct tcttatttaa taaagaaaaa ttactattaa tagctttaat
     5401 taactcttct tttgatgatg gaacagccat atctattgct ccttgtatag ttacacaact
     5461 atttctaccc tactatttcg agtgattcaa tcgtctggtt cacataacct accacctgtt
     5521 caaaatgctt atcgacaaaa aaatgatcgg cagcaggaaa tataatagtc cgcgtctttc
     5581 gtgtggtgaa tttttcccat gcaagtaatt catcctgcat taccagattg tcagcatcgc
     5641 catgaaatag cacgatcgga caggttaatg tgcgcgcctt ggcctgaaat acatactgct
     5701 catagagccg ataatcgttt ttaatgatgg gggtgaaaat tgtcat
//
LOCUS       DADLTF010000201         5721 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.203, whole genome shotgun sequence.
ACCESSION   DADLTF010000201 DADLTF010000000
VERSION     DADLTF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.203"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            67..585
                     /gene="mddA"
                     /locus_tag="JN749_003823"
     CDS             67..585
                     /gene="mddA"
                     /locus_tag="JN749_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HAY7370058.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            582..1031
                     /locus_tag="JN749_003824"
     CDS             582..1031
                     /locus_tag="JN749_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HAY7370059.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1032..1264)
                     /locus_tag="JN749_003825"
                     /pseudo
     CDS             complement(1032..1264)
                     /locus_tag="JN749_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
     gene            complement(1350..1523)
                     /locus_tag="JN749_003826"
     CDS             complement(1350..1523)
                     /locus_tag="JN749_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HAY7370060.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1718..1813
                     /gene="yncL"
                     /locus_tag="JN749_003827"
     CDS             1718..1813
                     /gene="yncL"
                     /locus_tag="JN749_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HAY7370061.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2215..3024)
                     /locus_tag="JN749_003828"
     CDS             complement(2215..3024)
                     /locus_tag="JN749_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HAY7370062.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3234..4659)
                     /gene="patD"
                     /locus_tag="JN749_003829"
                     /pseudo
     CDS             complement(3234..4659)
                     /gene="patD"
                     /locus_tag="JN749_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(4681..5387)
                     /locus_tag="JN749_003830"
                     /pseudo
     CDS             complement(4681..5387)
                     /locus_tag="JN749_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(5377..>5721)
                     /locus_tag="JN749_003831"
     CDS             complement(5377..>5721)
                     /locus_tag="JN749_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAY7370063.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1497 a         1446 c         1271 g         1507 t
ORIGIN      
        1 ccgttgcttc gggctgtttt cgatagtatt gataacatca tcatcattat tttattgagg
       61 ccagacatgt ccatccgttt tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat
      121 aaccacgccg tgttgtatac ggcggctatc tggaatgacc aaacggtgga tgcagataac
      181 cgcattgcct ggtttgaagc gcggaccata gcaggttatc cggtgctggt aagtgaagaa
      241 gatggcgtag ttacgggata tgcctcgttt ggcgactggc gtagtttcga tggttttcgc
      301 cataccgtgg aacattcggt ttatgtccat cccgatcatc agggcaaagg tctggggcgt
      361 aaattgttaa gccgattgat tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc
      421 gggatcgaat cgcaaaatca ggcttcgctg catctgcacc agtcgctggg atttgtcgtc
      481 accgcacaaa tgccgcaggt aggcactaaa tttggtcgtt ggctggatct gacatttatg
      541 cagttgcaac tcgacgagcg cactgaaccg gacgcgattg gatgaatcag tcgctcaccc
      601 ttgctttttt gattgccgcc ggaattggtc tggtagtcca gaacacgctg atggtgcgca
      661 tcacccagac ctcctctacc attctcatcg ccatgttgct gaactcactg gtggggattg
      721 tgctctttgt ttctatttta tggtttaaac aaggcatggc ggggtttggc gaactggtgt
      781 ccagcgtgcg ctggtggaca cttatacctg gcttgctggg ttcgtttttt gtcttcgcca
      841 gtatcagcgg atatcagaat gtgggagcgg cgacaactat tgcggtactg gtcgccagcc
      901 agctgattgg cggtctggtg ctggatatct tccgcagtca tggcgtacca ctacgggcgt
      961 tgttcggccc aatatgtggc gcaattttac ttgtggttgg cgcctggttg gtggccagac
     1021 gctcatttta atcagaggat ggtgccgcct ttggttaact gttcgtggcc gcttccatat
     1081 cttctttatg cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt
     1141 agcgatcttc ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat
     1201 gggcaatgac gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg
     1261 acatactttc tccttagcca cttgtgaaat ttatcgatta atccaaatga tataagtctg
     1321 cgtggtgccg tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg
     1381 aaactcatcg gccatgtggc ccccaccaga aaggcgctta agaaacggat gcgtttctta
     1441 tcgtgagaaa gaaaccaggt gataagaaat gcgattgctg ccataaccgc ataaaaaaca
     1501 agcatatgtt gatagagaga catgtacggg cgtccagcca tatgaataaa acgcgcgcaa
     1561 atatgcgcca ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa
     1621 acagcaactt gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt
     1681 actatacaca taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt
     1741 agtactgata aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg
     1801 ctggtttatt tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc
     1861 tgacagtacg caagatcttc tcatactctc caagtttgca acattatcct taatatttac
     1921 ttctttttgc tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata
     1981 caaacagctt actgattcaa acggaatctt gttttcacat ttttctatca cgaatacttt
     2041 atacgcgcgg gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga
     2101 tggcgtaaag tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg
     2161 aaattgcaaa ccccgccctg gttatcttgc ccatttatca tgcaaaacag ataattatag
     2221 gcctctgaat acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatt
     2281 tgatatcaat cgattcatct cttcaacaaa ataatttata tcatcacact tgttattatc
     2341 cggaagatct gagtagttat aattccacca ttttattctc aacagttcat caataacatt
     2401 tgaatcaaac ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg
     2461 cacatctttg gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc
     2521 acctttcaat acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg
     2581 tttggtaatg aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc
     2641 aaattcaccg ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc
     2701 cattattttt acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa
     2761 atggcaggtc ggaaatgaaa aagcgccgac actggagaaa tttttcacag gcattgttga
     2821 atattcttca gcaataacat tatcttcaat tgttatcttg tcaccttttg agaaaagaaa
     2881 taaattcctt tctttatacg acttcttccc cttatgattt aaaaagattt tattatccat
     2941 acaaaactca aagtgttttt tcgtccatct gaaggtaagg gttttatttt tatcttccgt
     3001 gaatttgtac aattgaactt tcattgttaa tacttctact tataaattat tagtttaata
     3061 acgatactct aaagccttat gctaatcaac tgtgacagga tgaaaaatcg ccatcaaaca
     3121 tttagcctaa tttttacgcc agttattaat gtaatcattc taatacttaa ccgccattaa
     3181 actcagaacc taaaacgata gtggccgtcc tataacagcc actaacatct aacttaatgt
     3241 ttaaccatga cgtggcggac gacggtgtaa tcctccagcc cataaagtga catatccttg
     3301 ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac ttaccagcat gaaatgggta
     3361 ttgacccagg tacaaccata ttgcagccgt gcgctgacgc gatgcgccct gcccacatct
     3421 ttcgtccata ccgaagatgc aagtccgtac tggctgtcat tcgcccagtt caccacctgt
     3481 tcttcgttgt cgaagggcgt aacactcact actggaccaa atacctcttt ttgcaggatg
     3541 gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat agtaatagcc attacccttg
     3601 cgcttttcac cgccagtgat cactttgatg tgccctgtcg ctttcgcctc ttctactgcc
     3661 ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc caagctccgt agactcgtca
     3721 tccggcgcac cagattttaa cgttgccacc gcaggcaccc agtttttcca ccagcgtatc
     3781 gtaaatgcct ttttgcgcgt agatccgaca agccgcagta caatcctgtc cagcattgta
     3841 atagccaaat gtacgtacac cttcgaccac tgcttcaata tccgcatcat caaaaacaat
     3901 cactggcgct ttgccaccaa gttccatatg agtacgctta atggacgacg cggtatggct
     3961 gatgatgtgc tcgccggtgg cgatagagcc cgtcagcgac accatccgca ctttgggatg
     4021 accggtcagc ggatcaccca ccgttttgcc tctgccaaac agtatgttaa tcacgcctgc
     4081 cgggaagata tctttcgcca gctctgccaa cttcaacgcg gtcagcgggg taatttctga
     4141 tggtttaagc actacgcagt tccctgccgc cagcgccgga gcaagtttcc acgcggccat
     4201 catcagcgga taattccacg gtgcgataga agccacgccc cccaacggat cgcgacggat
     4261 catcgaagta tgaccttcaa aatattcacc tgccgccaga ccattcagac agcgcgccgc
     4321 acccgcgaaa aagcgaaaaa catcgacaat cgccgggatt tcatcattga acgcactatg
     4381 cagcggtttg ccacaattac gggattccag ttcggcaaaa acctgaccat tttcttcgat
     4441 aacatccgcc agtttcagca ggcattccgc acgcgctttc ggtgtggttt gcccccattc
     4501 agcaaatgct gcatctgccg cacacacagc agcatcgacc tgctctgcgg atgcctcggc
     4561 aatctccagt aaaacgtccc ccgttgccgg attatagaca ggctgttttt ccccttcgcc
     4621 gctaaccagt tctccgttaa tcagtaactt atgttgcata gattattcct gtatcagtgg
     4681 ttattgacca ttgtcgcctt cgcgggttag ccaccaggcc cccaggatcg gcaaggttgt
     4741 taccaacata accagcagtg ccaccacgtt ggttaccggt acatcacgcg gtcgcccaag
     4801 ctgattgagc aaccacaacg gtaacgttcg ttcatgacct gccgtaaagg tcgtaacgat
     4861 gatttcatcg aacgacaagg caaacgccag cattcctcct gccagtaacg ccgaactgag
     4921 attcggcaac actacgtagc ggaaggtttg ccagccattg gccccaagat ccattgacgc
     4981 ctcaaccaga ctccacgagg tgcggcgaaa acgggcgatg acattgttaa acaccaccac
     5041 tacgcaaaaa gtcgcatgac cgaccacgat ggtgaaaaat cccggctcca gattgatggt
     5101 tttaaaggcg gttaatagcg ccagaccagt gacaatgccc ggcagcgcaa tgggcagcag
     5161 caataacagc gaaatggcgt ttttgccgaa aaagtctcgt ctccacagcg cggcagctgc
     5221 cagcgtccct aacaccagcg cagcgtcagg ccctgcggtg gaaaactaaa cgccgcatct
     5281 tcagtgttaa acgcataggc ggcgattatc aggatgggaa aatgtaggaa aacaacgccg
     5341 ccccaggccg ccagtttgag gaaaaacggt gcgcgttcag agtgcatcga acgctcccag
     5401 acgtttcacg aacgccaggt acagtgcgat gagaataatc ggcaccaggg tgaatgccgc
     5461 cgccatcggc atattgccaa tcgccccctg ctgggaataa accatattgc cgataaaata
     5521 tcctggagga ccaaccagct gcgggacgat aaaatcgccc agtgtgagtg agaaggtaaa
     5581 gatagagcca gcggcaatac ccgggattgc cagcggcagc accacatagc gaaaggtttg
     5641 tcgtggacgt gcgccgagat cagccgacgc ctgcaacaat gacggcggca aacgctcaag
     5701 cgccgcctga acgggcagga t
//
LOCUS       DADLTF010000202         5708 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.204, whole genome shotgun sequence.
ACCESSION   DADLTF010000202 DADLTF010000000
VERSION     DADLTF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.204"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            57..1525
                     /locus_tag="JN749_003832"
                     /pseudo
     CDS             57..1525
                     /locus_tag="JN749_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(1561..1764)
                     /gene="ydfZ"
                     /locus_tag="JN749_003833"
     CDS             complement(1561..1764)
                     /gene="ydfZ"
                     /locus_tag="JN749_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HAY7370064.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1942..2628)
                     /locus_tag="JN749_003834"
     CDS             complement(1942..2628)
                     /locus_tag="JN749_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7370065.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSNWFSEE"
     gene            complement(2717..3463)
                     /gene="ydfG"
                     /locus_tag="JN749_003835"
     CDS             complement(2717..3463)
                     /gene="ydfG"
                     /locus_tag="JN749_003835"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HAY7370066.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3600..5645
                     /gene="dcp"
                     /locus_tag="JN749_003836"
     CDS             3600..5645
                     /gene="dcp"
                     /locus_tag="JN749_003836"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HAY7370067.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1427 a         1357 c         1475 g         1449 t
ORIGIN      
        1 tttttattca tataacaaaa catatagctt gccatattta aatttaagga attgttatgg
       61 gaaataattt gttatcagca aaagcgacac tccctgttta tgatcgtaat aacctggccc
      121 caagaattgt tcatttaggc tttggtgcat ttcaccgtgc gcatcagggc gtgtatgccg
      181 atattcttgc tacagaacat ttcagtgact ggggatatta tgaggtcaac ttaatcggcg
      241 gcgaacagca aattgccgat ttacaacagc aagataatct ttataccgtt gcggaaatgt
      301 cggccgatgc gtggacggct cgcgtcgttg gcgtcgttaa aaaagccttg cacgtacaga
      361 tggatggctt agaaaccgtg ttggctgcga tgtgtgaacc gcaaatcgcg attgtctctc
      421 tgacaatcac cgaaaaaggg tatttccact ctccggcgac cggacagtta atgctcgatc
      481 acccgatggt cgctgccgac gtgcaaaatc cccaccagcc gaaaacagca acatgggtga
      541 ttgttgaggc gctggctcgc cgtaaagcgg caggacttcc cgcattcccg catttaccgt
      601 catgtcatgt gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta
      661 cgcgcaagcc gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc
      721 aacaatggtg gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca
      781 acttaccggt gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat
      841 cgaagataac tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag
      901 tgatgtgctg ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct
      961 ggcatatctg ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca
     1021 ttatcgtcat gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca
     1081 gggcgttgat ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt
     1141 acgtcatcga acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt
     1201 ggattctgtt cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt
     1261 cgcgggttgg atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag
     1321 tgacccactg ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg
     1381 cgtccagtca ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt
     1441 gtttactgca aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac
     1501 cgtggcgaaa tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac
     1561 ttagttcatg ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac
     1621 aactacggtt tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag
     1681 gatcttgcca gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt
     1741 acggttacga tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc
     1801 acgtaacggg cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag
     1861 ctcacatctt ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg
     1921 gatgggatga gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat
     1981 ctggcgcacg gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc
     2041 gccatcacgt ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg
     2101 tggagaaaca tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc
     2161 aatggtatcc cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc
     2221 atcaagttca aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag
     2281 attttgttcc agttggtagc actggctttc ggtaatcatg ctcgccgccc gacgcgccac
     2341 cgcgcactca atggcctgac ggataaaact gccgttgcgc acctgggcca tggaaatttt
     2401 gttgacgtag ctgccacgct gcggacgaat ttgaatcagg ccgttttccg ccagtttaat
     2461 aaaggcttca cgaaccggct ggcgtgacac attgaaacga acagaaactt ctttttccga
     2521 caacggtgtg cctggtgcaa tcaggcaatg gacaatgtcg cgacgaagaa tacgataaat
     2581 ctgctgattg acaggctgtg tgggattaag ttgcgtttcg acggtcattc gttcttgctt
     2641 gtgagtgagt taactgcatg agtctaccac ttttttgtga caagcaataa cccggcagtt
     2701 acgccgggta taaaaattac tgacggtgga cattcagtcc ggcatagctt tgggtaaccg
     2761 gcatcatttc cagggtattg atattgacgt gagcaggcag cgttgacacc caccagacgg
     2821 cttcgctgac atcttctggc gtcaatgcaa cggtattttg ataggttttt tccgctttac
     2881 cgtcatcgcc tttaaagcgg acattggaaa actcggtgcc acccaccaga cccggttcga
     2941 tgtcggtgac gcgcaccgcc gtaccatgca gatccgtacg cagattcagg ctaaactgac
     3001 gaacaaacgc tttcgtcgca ccgtaaacgt taccaccggc atacggccag ctgcctgccg
     3061 ttgagccaat gttaataata tgaccatgat tacgttcaac catacccggt aagacggcgc
     3121 gcgtcatata tactaggcct ttgttgttgg tatcaatcat cgtttcccag tcttcaacgc
     3181 tggctttatg cgcaggctcc atgcccaacg ccaggccggc attatttacc aggatatcaa
     3241 tattgctcca ctcggcaggg agcgatgcca gcatctcttc aatagcggcg cggttgcgaa
     3301 cgtccagttg ggcgatatac agattatctc ccagttcgtc ttttaactcc tgcaaccgct
     3361 cctggcgacg gccagtggcg ataactttat gcccttgttg aataaaacga cgagtaatgc
     3421 attcaccaaa acctgccgtt gctccagtta ctaaaacgat catctcactg ttcctcaacg
     3481 cttttgtgtg tcaataacat agcacgtgat gcttaagagc gggtaatact ggtttgcaag
     3541 attgcactcc gttatcagta agcctacgct atggaacacc tcgcgttcag gagaagaaaa
     3601 tgacaacaat gaatcctttc cttgtgcaaa gcacactgcc gtatctggct cctcattttg
     3661 atcaaattgc caatcatcac tatcgcccgg cattcgatga gggaatgcaa caaaagcggg
     3721 cagaaattgc tgccatcgcg cttaacccgc aaacgcctga tttcaacaat actattctgg
     3781 cactggaaca aagcggagaa ttaattaccc gcgttaccag cgtctttttt gcgatgactg
     3841 cggcgcatac caatgatgaa ttacagcgtc ttgatgaaca gttttccgct gaactggcgg
     3901 aactggctaa tgatatctat ctgaacggtg aattattcgc gcgggtagat gctgtctggc
     3961 agcgccgtga atccctgggg cttgatagtg aatccatccg cctggtggag gttattcatc
     4021 aacgttttgt ccttgccgga gccaaacttg cacaagctga taaagcaaaa ttaaaagtac
     4081 tgaatacaga agctgcgacc ctgaccagcc agtttaacca gcggttactt gcagcaaata
     4141 aatccggcgg tctggttgtg aacgatatcg cgcagctggc aggaatgagt gagcaagaga
     4201 ttgcgctggc ggcagaggcg gctcgcgaga aaggtctgga taacaaatgg ctgattccgc
     4261 tgctgaatac cacccaacaa ccggcgcttg ccgaaatgcg cgatcgtgcg acgcgtgaaa
     4321 aactgtttat tgcgggctgg acgcgagcgg aaaaaaatga tgccaatgat acccgcgcta
     4381 tcattcaacg tctggtagag attcgcgcac agcaggcgaa actgcttggt tttcctcatt
     4441 atgccgcatg gaaaatcgcc gatcagatgg caaaaacgcc agaagcagca ctcaacttta
     4501 tgcgggaaat tgttccagcg gcgcgtcaac gtgcgagcga tgaattagcc tccatacagg
     4561 cggttatcga taagcagcag ggcgggttta gcgcgcagcc gtgggactgg gcattttatg
     4621 cggaacaggt acggcgggag aaatttgatc ttgatgaggc gcagctcaag ccatattttg
     4681 aattaaacac ggtgttaaat gaaggtgtat tctggaccgc gaatcagctc ttcggtatta
     4741 agtttgtcga acgttttgat attccggtct accatcctga cgttcgtgtg tgggaaattt
     4801 ttgatcataa tggcgtggga ctggcgttat tttacggtga tttcttcgcc cgtgattcaa
     4861 aaagcggcgg tgcatggatg ggcaattttg ttgagcaatc aacgcttaat gaaacgcatc
     4921 cggtaattta taacgtctgc aattatcaga aacccgctgc cggtgagcct gcgttgttac
     4981 tctgggatga tgtaataacc ttattccatg aatttggtca tacgctgcac ggcctttttg
     5041 cccgccagcg ttatgccacg ctttccggca ccaacacgcc gcgtgatttt gtcgaatttc
     5101 cgtcgcaaat caacgaacac tgggcaacgc atccgcaggt attcgctcgc tacgcccggc
     5161 attatcagag cggggcagca atgcctgacg aactgcaaca gaaaatgcgt aatgccagcc
     5221 tgttcaacaa agggtatgag atgagcgaac tgcttagcgc cgcacttctc gatatgcgct
     5281 ggcattgcct ggaagaaaac gaagcaatgc aggatgtcga tgattttgaa ttgcgggcgc
     5341 tggtggcgga aaatatggat cttcctgcta taccgccacg ctatcgcagc agttatttcg
     5401 cccatatttt tggtggcgga tatgctgcag gttattacgc ttatctgtgg acgcaaatgt
     5461 tggccgatga tggttaccag tggtttgttg agcagggcgg attaacgcgt gaaaatgggc
     5521 agcgttttcg cgaggcgatc ctttccagag gtaacagcga agatctggaa cgcctgcatc
     5581 gacaatggcg cggtaaggct cctcagatta tgccgatgct gcaacatcgt ggcttgaaca
     5641 tataaacacc accaatggct ggcctggaga tatcgccagg ccagcctggt tatagcagta
     5701 attgcagc
//
LOCUS       DADLTF010000203         5695 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.205, whole genome shotgun sequence.
ACCESSION   DADLTF010000203 DADLTF010000000
VERSION     DADLTF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.205"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(76..495)
                     /locus_tag="JN749_003837"
     CDS             complement(76..495)
                     /locus_tag="JN749_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370068.1"
                     /translation="MHQLRHPLLGCVFCNECDPHPFAPDPLQIFHHYYEWVRPCFPIR
                     AGNRFSRSLRWSGPGSCCLHTGCRAVSKQVSSALIRGRLYGPILASSKISMLHRTVYF
                     RSASRTPRDSRSLPFPESLTTSFLRMKQHRVVWSVLL"
     gene            545..1723
                     /gene="ltrA"
                     /locus_tag="JN749_003838"
     CDS             545..1723
                     /gene="ltrA"
                     /locus_tag="JN749_003838"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7370069.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDVTRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1806..1985
                     /locus_tag="JN749_003839"
                     /pseudo
     CDS             1806..1985
                     /locus_tag="JN749_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2015..2497)
                     /locus_tag="JN749_003840"
                     /pseudo
     CDS             complement(2015..2497)
                     /locus_tag="JN749_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2753..3733)
                     /locus_tag="JN749_003841"
     CDS             complement(2753..3733)
                     /locus_tag="JN749_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAY7370070.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3733..4932)
                     /locus_tag="JN749_003842"
     CDS             complement(3733..4932)
                     /locus_tag="JN749_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAY7370071.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5254..5592)
                     /locus_tag="JN749_003843"
                     /pseudo
     CDS             complement(5254..5592)
                     /locus_tag="JN749_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1568 a         1302 c         1341 g         1484 t
ORIGIN      
        1 taacgccggt tcggaaattg cagcataagt tatccatgct ctgaggtggg tggaggtggg
       61 ccctccggtc agggtttaca ggagcactga ccaaacgacc cggtgctgct tcattctaag
      121 gaatgaggtg gtgagcgact cgggaaaagg cagagatcta ctgtcacgtg gggttcggga
      181 agctgaacga aagtaaactg tccgatgaag catcgaaatc ttagatgatg ccaaaatggg
      241 gccgtacagc cgtcccctga taagcgcaga agacacctgc ttactgactg cgcggcatcc
      301 ggtgtgaagg cagcaggatc ccgggccaga ccatctcagg gaacgtgaga acctgtttcc
      361 ggcacgtatc gggaaacagg ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg
      421 gtctggagcg aagggatggg gatcacattc attgcagaag acacaaccca aaaggggatg
      481 acgtaactga tgcaacgaaa atcatttgaa ataccaaaag cactggtctg ggcatcatat
      541 ctggatgtgc gtcgcaacaa gggagccccc cggatgcgac gggcagactt tgaaaatgtt
      601 cgaccagcaa cgtgatggca atctatacaa gatctggaac cggctatgtt cgggaacatg
      661 gtttccgcca ccagtgctgg aaaaacggat cccgaagtcg aatggcaagg aacgcattct
      721 ggggatccca acagtatccg atcgaattgc tcagggagcg ataaaacttt tcatggaaga
      781 aaagcttgac ccgattttcc atgctgattc atatggttac cgaccaggca aatcagctca
      841 tgacgctctg aaacaatgtg ccatccggtg ctggcgttat agctggatac tggaagtgga
      901 catcagtgcg tttttcgacc atgtgagaca tgatctggtg ctcaaagctc tggaacacca
      961 cgggatgcct aaatgggtca tcctgtactg tcgacgctgg atggaagcgc caatgcagag
     1021 ttgtgaaaat ggagaattaa taacccgaac acgaggcact ccgcagggcg gggtcattag
     1081 tccgctactt gctaacttgt tccttcacta tgcatttgat ttgtggatgg aaagagaata
     1141 tcggggggta ccgtttgaga ggtacgctga cgatattgta gtacattgtt cacgaatgag
     1201 tgatgtaaca aggctaaaga acaggttatc ggagcgcttc tcggaagtcg ggctggtcct
     1261 gaatgcaggg aaaacgaata tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac
     1321 gagtttcacc tttctcggat atgacttcaa agtgcgtacg ctgaagaatt tcaaaggcga
     1381 acggtaccga aaatgcatgc cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac
     1441 aatcaagaag tggcgtatac atcgctcaac agctgagagt ttgctggatt ttgcgaggcg
     1501 ctacaatgcg atagtgagag gctggatcga gtactacgga aagttctggt ccagaaattt
     1561 caactatcga ctgtggagtg caatgcagtc acgtctgctc aagtggatgc agtctaaata
     1621 cagactttcg aaccggaagg ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa
     1681 gctatttgtt cactggtatt tactacgtgc atcaaatgag tgatcaagag ccgtatgacg
     1741 cgagagtgtc acgtacggtt ccgtgagcag ccggggagtg aaattctctc cggctgactc
     1801 gactcgcggg ttccgacagc gatgacgaac atgcggcggt gttgtactcg ctgatcggca
     1861 catgccgtct gaacaatgtg gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc
     1921 aggactggcc ggcaaaccgg gtacgtgatc tgttgccctg gaaagttgat ctgagctctc
     1981 agtaaatatc aatacggttc tgacgagccg cttacttttg gcgtaattct gctgcgcctt
     2041 tactgaacac aatgccgtat tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc
     2101 tttccattga aaccaaccga cgcatattca aaaaagtctg gacgtcctgt tgctccagag
     2161 tttttattgg tacgggtcgt atagttccat gttggcaggc ctcagctatt gcctgtgcgt
     2221 cgttataatc atttttctgg caccgctgat aagctttaac atgttgtgcc ggaagcaata
     2281 ttacatcatg gtcgagtttc ctgatttccc gggcccagta atgagatgcc ccgccagcct
     2341 ccatcgcaac agtcatttta ggctgttgca cctgccagtt aagcagttga ttgcgagaaa
     2401 gtttggtctt tcctgcgggt ttgccatgag cattgatact gaaaagataa aagttcgttt
     2461 tagccagatc aatgccggtg attttctgtt cagacatggt atcccgctta cttttgtggt
     2521 taaagtttat ccatcatggc gcaattgaag gctgccgtga aagaggggga agactatctc
     2581 atcggttact gttaaacggc taagagaaac tatctttttc aagcaaagaa gatacggtga
     2641 aacaggcgat ttcaccgtga aatactgttc aaaaaaaacg ctaataaaag caaaatttct
     2701 tttaaaacag ccaattaaaa actggtgagt tgctggcgaa aaacttgaat tttcacagcg
     2761 aattcagatt atcttttagt acaagagcaa tcatacgatc taacttatcc tgaacttcca
     2821 acggtaatct gccgaattca tatgagaacg tacgtccctt tactcgtttt cgagcaaatt
     2881 tatctttact atcaaatttc cataacaaag ttacaaccgc tttatccttt actcctgaat
     2941 caagcaatga agacatttcc ttggttataa gccgcataat actgttttta acctcgtctt
     3001 cagaaagatt gtcatcagca gtaataagct caatactcgg cgagatattc ttcacaactt
     3061 catcaataga tatatcaccc agcctcagtc gttcacttaa ttcagccagt tgcctgtaat
     3121 cggcgtatgt cagctcagac tgaacaggaa aaagagacac aaaatcttta ggtacggacg
     3181 ctgcttggag ggcacgagtc accttagctg ccgacaatcc ctctctttcc gcaatttgct
     3241 tctggttcat accggcctct ttgagacgta ctaaacgcaa cccaatttca cgaatattat
     3301 gttcaagtga cgtctgcaaa tctttcgcca gatgctgagc ttcagaaaca gtaagctcat
     3361 catccgttac aagtacacgt aatccaacct tgcatagcaa agctgctgcc cgacgacggg
     3421 aaccatccaa aatctcaata agatcgccag tccgaatccc aatacatgga tagaattgtt
     3481 gtaaacgtat agttctggtg atgtctttta gtgagtccgg cgtcagggct gtctgttcgc
     3541 ggccattcac ctcttcaacc acaaaagttt gagtgtccac tttgtcatga tcaaccttga
     3601 tctcagtaaa ttttgccttt cggccagtat tgagggtaaa tacatgaact ggagccgata
     3661 tttcttcagt attattcagg atatttgtat tcagcgtacg gccaatagtc ggtcggtgct
     3721 ttctgttttc catcagtagt tggcccttat aaattcaata cgatcaaaaa ctgctttagc
     3781 gaaatcttct gctgccatac gtgcattttt caaagcttca ccactgccaa cataagtaac
     3841 aggattcgca gatatcacgg tatcaaacga ctcgccgctt ctctcaaaac catcaagacg
     3901 tggcattgaa acatcaagca tgtcaccacc gaaaatctct tttgtcaggc tatggcagta
     3961 tttatgatct gacttattcg ccagtttcga catgaagcca atatttgcct gcaaacggca
     4021 tgaacatcca tcctgttcaa taatctgtac aagttcaggt aaacgagcca aatatttaag
     4081 cgtggaatgg aaatcaactt gtgctggcgg caccggtgta aacatgatat ctgcggcagc
     4141 aatggcattt ttcaaaaatg cgtccagatg cgggcctgta tcaataagga tgaagtcgaa
     4201 atcatgcttc agcttatcaa taatattctc acgcaatatc gcgtgtttgt tttgtcctaa
     4261 tagatgctcc tcgcacagag tatcccagtt tgatgcaata aaggcgtcgt caatagaagc
     4321 cggcataaca taaacgccag ggataactga cggaacgata aaatcttcca gtaattcctc
     4381 acgactcacg ttctgcaaca tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg
     4441 caagtagttc agaaacatag tggctgatga ctggggatct aaatcaagga gcagaatacg
     4501 taaatcttct gacagcaggt gaggatgagc acgcagggcg tgagctacgg aaacactact
     4561 cacggtttta gacacaccgc cttttagact accgatgaat atggaatatg cctcactata
     4621 acgatcgcga tatttaggaa tgccacgatg tgcatagata tcgataatgt tctggatggt
     4681 taatgcatat tttgtcgcgg ttcctgctgg gcgtttttca aactgatagc cctgagcctc
     4741 catctcaccg actgcaccat ccacgcttgc gcgcgttaat tttggaagct tagctacagc
     4801 agactttgaa tagacttgat aaaaaacatc agcctgaaac tcctgctttt gctcctgaat
     4861 ctgttttgtt agggcgagca acattttatc tgcacgctgc gcaaccttac ttaactgctc
     4921 aaaactagtc attccccctc cgtgctatgt aggattttaa cttattacaa aaaaaactgc
     4981 attagcaggg taatgcatgc aagtacatat tgtacatgct tatattaaaa atgcgcgcat
     5041 tgctgattta ttaatcatcg gaatgaatat tactgtaaga tagtatgcct ttccgggaga
     5101 aagacgctga tttcccccga ataatccagt tcaccaccac actccggaca acagctttct
     5161 tcaggctcca gatggtgtat ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt
     5221 gactgtcgca acaggcgggg gtagtgcttc caatcagtag aacatgtgct tttcaatgaa
     5281 ggagccgatg actttttcgt ggatttccac ggaacgcgag aagcagattg ttttacgtgc
     5341 cagccgcttg atgcgggtgc agagggtccg gttgttacgt tcaatacgtt gcgtgaagat
     5401 tttgccggtc aggtgtttct ccttcggcac ttctctggcg tagctgcccc agtcatcgct
     5461 ggtcaccatg ccgatacaga acggagtcaa cagggctaac agttcccgac aggtctcatc
     5521 attccgtggc ccgaaagtgt aagccaacac accgccagtt ttagtgttgt acgcatacca
     5581 gagccagtgt tgtgtcaacg acggatgaaa agtgctccac ttatatctcc accaacggcc
     5641 caatattgat ccaccgtttt actcaggatt agcttctgct ataaccccgg ccttt
//
LOCUS       DADLTF010000204         5600 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.206, whole genome shotgun sequence.
ACCESSION   DADLTF010000204 DADLTF010000000
VERSION     DADLTF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.206"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..410
                     /locus_tag="JN749_003844"
     CDS             <1..410
                     /locus_tag="JN749_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7370072.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /locus_tag="JN749_003845"
                     /pseudo
     CDS             442..1056
                     /locus_tag="JN749_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JN749_003846"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="JN749_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HAY7370073.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWFSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1380 a         1402 c         1330 g         1488 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggacagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagaa
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actgtacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aagggccagt ttggatatcg
//
LOCUS       DADLTF010000205         5553 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.207, whole genome shotgun sequence.
ACCESSION   DADLTF010000205 DADLTF010000000
VERSION     DADLTF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.207"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..638
                     /locus_tag="JN749_003847"
     CDS             <1..638
                     /locus_tag="JN749_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370074.1"
                     /translation="GACIVLMELMMMVQLWWSVWLCWPVLSLLIVGRGCGGLGAITTG
                     KDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNAV
                     LDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALG
                     LSRSALVVAAWLLCYGHCKTVDEAISFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JN749_003848"
     CDS             complement(692..1297)
                     /gene="azoR"
                     /locus_tag="JN749_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAY7370075.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1498..5400
                     /gene="hrpA"
                     /locus_tag="JN749_003849"
     CDS             1498..5400
                     /gene="hrpA"
                     /locus_tag="JN749_003849"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HAY7370076.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLSFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1356 c         1540 g         1281 t
ORIGIN      
        1 taggcgcgtg cattgtgttg atggagctaa tgatgatggt tcagttatgg tggtcagtct
       61 ggttatgttg gccagtatta tcgctactca ttgttggccg tgggtgcggt gggcttggcg
      121 cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac tggctgacat
      181 tgccctggcg catcgggatg tggctatcta tgcgttggtt ttgtcgtcgc ctggagccgg
      241 tgagcaaaat gactgctggt gtttatttaa gggcgtttcc acgacatatt ccggcacaga
      301 atgcggttct ggacgtcacc tttgaattcc ctcgtggacg agcgacaaaa gatcgactct
      361 atttctgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc cgacaggccg
      421 tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat tgcgcattgg
      481 gattatcgcg cagtgcgctg gtagtggcgg catggctgtt atgttacgga cactgtaaaa
      541 cagttgatga agcgattagc tttattcgag ccagacgctc gcatattgtg cttaaggaag
      601 agcacaaagc gatgttgaaa ttatgggaaa acaggtaagt ggattgagat gtggactgaa
      661 tatctacagt ccacatcaag accgtgtccg gttatgcagc aacaatgctg tcgatggctg
      721 cttttgcgtc agactgtgct ttcgctgcca tttccggacc gtatgcgatc ccttcggcga
      781 agacaaattt cacatcggta atgccgataa agccgaggaa cgtggacaga tacggcgtca
      841 ccaggtccgt tggtccatct ttgtggatcc caccgcggct ggtaataacg atggcttttt
      901 tacccgttac cagaccttcc ggaccgttct cggtatagcg gaaagtaacg cctgcgcgtg
      961 caaccaggtc aaaatagttt ttcaactgag ttgagatgtt gaagttatac attggtgccg
     1021 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     1081 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagagcgcca accagttcgc
     1141 catccagtac cggaatcgga tttgcagcta ggtcgcgaac ggtgatttca tcagcggagt
     1201 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     1261 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatgtttga
     1321 atggacgatg ccccgttgct tgctgacact ttattcacaa tcctgccaca gagatagcgc
     1381 aataaatcga agtctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     1441 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     1501 acagaacaac aaaaattgac ctttactgcc ttgcaacagc ggctggattc gctgatgctg
     1561 cgcgacagac tgagtttttc tcgccgtctg cattgtgtta agaaggttaa aaatcctgat
     1621 gcacaacagg ccattttcca ggagatggcg aaagagattg accaggcggc agggaaagtc
     1681 ctgctgcgtg aagcggcacg accggaaatc acttatcctg acaatttacc ggttagtcag
     1741 aaaaaacagg acatactcga agcgattcgt gatcaccagg tggtgatcgt cgccggggaa
     1801 accggctccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcggtatt
     1861 aaagggctga tcggtcatac ccagccgcgt cgcctggcgg cacgaacggt ggccaaccgt
     1921 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     1981 gatcacgtaa gtgataacac gatggtcaag ctgatgaccg acgggatcct gctggcggag
     2041 atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     2101 cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc gcggcgtcct
     2161 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     2221 aataatgcgc cgattattga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     2281 ccgattgttg aagaagccga tgacaccgag cgcgatcagt tgcaggcgat ttttgacgcc
     2341 gtagacgaac taagccagga aagccccggc gacattctga tctttatgag cggcgagcgg
     2401 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     2461 ccgctttatg cgcggctttc gaacagcgaa cagaaccgcg tgttccagtc gcacagcgga
     2521 cggtgcattg tgctggcgac caacgtcgcg gaaacctcgc tgaccgtacc ggggattaaa
     2581 tacgtcatcg accccggtac agcgcgtatc agccgctaca gctatcgcac caaagtgcag
     2641 cgtttgccga ttgaacccat ttcgcaggcg tctgccaatc agcgtaaagg ccgctgtggt
     2701 cgtgtgtccg aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     2761 gagtttaccg atccggagat tctgcgtacc aacctggcct cggttatttt gcagatgacc
     2821 gcgctggggc tgggcgatat cgctgcgttc ccgtttgtcg aagcaccgga taaacgcaat
     2881 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     2941 agcgcctata aactgacgcc gctcggtcgc cagctctcgc agttgcctgt cgacccacgt
     3001 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     3061 acgtccgcgc tctccatcca ggatccgcgc gaacgtccga tggacaaaca gcaggcatcg
     3121 gacgaaaaac atcgtcgctt ccacgacaaa gaatccgact tcctcgcgtt tgtaaatctg
     3181 tggaattatc ttggcgagca gcaaaaggcg ctttcttcca acgccttccg tcgcctgtgt
     3241 cgtaccgatt acctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     3301 cgccaggtgg tgaaagaact tggcattccg gtcaacagcg aaccggcgga gtatcgtgaa
     3361 attcacatcg ccttactgac cggtttgctt tcccatatcg gcatgaaaga tgccgataaa
     3421 caggaatata ccggtgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     3481 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     3541 cgcattgctg cgcgtatcga cccggaatgg gtagaacccg ttgctcagca tttgattaaa
     3601 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     3661 gtcactgttt atggtttgcc gattgttgct gcgcgcaagg tcaactacag ccagatcgat
     3721 tcggcgttat gtcgtgaact ctttattcgc cacgctctgg tggaaggtga ctggcagacg
     3781 cgtcacgcat tcttccgtga aaacctgaaa ctgcgggccg aagtggaaga gctggaacac
     3841 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     3901 cgcatcagcc acgatgtaat ctccgctcgt cacttcgata gctggtggaa aaaagtcagc
     3961 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     4021 aaaatcagca agctggatta cccgaacttc tggcatcagg gcaatctcaa gctgcgtttg
     4081 agctatcagt ttgagccagg cgcggacgcg gacggtgtga ccgtacatat tccgctgccg
     4141 ttacttaacc aggttgagga aagcgggttt gaatggcaga tccccggtct gcgccgcgaa
     4201 ttggtgattg ctctgattaa atcgttgccg aaaccggtac gccgtaattt tgtgcctgca
     4261 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggagttacc gttgctcgac
     4321 agccttgagc gcgagttacg gcggatgacc ggcgttaccg ttgaccgcga agactggcac
     4381 tgggatcagg tgcccgatca cctgaaaatc accttccgcg tggtggatga caaaaacaag
     4441 aagctaaaag aagggcgctc gctacaagat ctgaaagatg cgctgaaagg caaagtgcag
     4501 gaaacgctgt ccgcggtggc ggatgacggt atcgagcaga gcggcttaca tatctggagt
     4561 tttggtcagc tgccggaaag ttacgaacag aagcgtggca actataaagt gaaggcgtgg
     4621 ccggcgctgg tggatgaacg cgacagcgtg gcgatcaaac tgtttgataa cccgctggag
     4681 caaaaacagg caatgtggaa tggtcttcgc cgtctactgc tgctaaatat ttcctcgccg
     4741 atcaagtatt tacatgagaa gttaccaaac aaagccaagc tggggctgta ctttaacccg
     4801 tatggcaaag tgctggagct gatcgacgac tgtatctcct gcggtgtgga tcaactgatc
     4861 gacgccaatg atggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtg
     4921 cgcgccgaac tgaatgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     4981 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcactgggg
     5041 ctttctgaca ttaaagcgca gatgggcggg ttggtatatc gcggttttgt cactggtaac
     5101 ggcttcaaac gtctgggcga cacgctgcga tatttgcagg cgattgaaaa acgactggaa
     5161 aaactggcgg ttgatccaca tcgcgatcgt gcacagatgc tgaaagtcga aaacgtccag
     5221 caggcgtggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
     5281 aaagagatcc gttggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
     5341 acgccttatc cgatttcaga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
     5401 ccctgctatt tgcctaataa atctaaaacc cggtaagcat ttagcgccgg gttttttatt
     5461 aattctaaaa cggtaaggac aaaaattcag gaattcagaa aaatacaatt ctcagctgca
     5521 agatgaataa tgtttatcta cagcatttcc tta
//
LOCUS       DADLTF010000206         5548 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.208, whole genome shotgun sequence.
ACCESSION   DADLTF010000206 DADLTF010000000
VERSION     DADLTF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.208"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..119)
                     /locus_tag="JN749_003850"
     CDS             complement(<1..119)
                     /locus_tag="JN749_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HAY7370077.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
     gene            complement(430..534)
                     /locus_tag="JN749_003851"
     CDS             complement(430..534)
                     /locus_tag="JN749_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HAY7370078.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            complement(809..1486)
                     /gene="kdpE"
                     /locus_tag="JN749_003852"
     CDS             complement(809..1486)
                     /gene="kdpE"
                     /locus_tag="JN749_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HAY7370079.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(1483..4162)
                     /gene="kdbD"
                     /locus_tag="JN749_003853"
                     /pseudo
     CDS             complement(1483..4162)
                     /gene="kdbD"
                     /locus_tag="JN749_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
     gene            complement(4155..4727)
                     /gene="kdpC"
                     /locus_tag="JN749_003854"
     CDS             complement(4155..4727)
                     /gene="kdpC"
                     /locus_tag="JN749_003854"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HAY7370080.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAVAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            complement(4736..5548)
                     /gene="kdpB"
                     /locus_tag="JN749_003855"
                     /pseudo
     CDS             complement(4736..5548)
                     /gene="kdpB"
                     /locus_tag="JN749_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
BASE COUNT         1318 a         1551 c         1436 g         1243 t
ORIGIN      
        1 actgataaaa taatggcggc aacgtcaata taattacttt cgtcaatatt cacgcatgca
       61 cgatgagtgg aaaaacaatc cgggcaagaa cggctaacgg caatttttaa ttctgacatt
      121 ttattcatct ctttatttca ggtaatagca ggtcctcaat ttctcaaagg agaaatcgat
      181 cacgccggaa aaaaagcaaa gctccaccgc tgataaataa atcagtagtt tgctttttgc
      241 agatgcaata acccagacca gcccggtaac tggctatact cgtcatacct caagttgcaa
      301 gtgctgcgac tcgaattatt tagagtttaa aagggttagc agtatatgag caccgaggaa
      361 tacaggtatt aacctgtaat gcttaaagtc agggagacct gtacgttggg gtgcccttta
      421 gtcagaagac tagcgggcat taaagaaatg aaagtcaaaa ctattacgat gggcaaactt
      481 catgatatgg gttgtccgcc ttatatgagg cattaagcgg ttattatttt ccatttacct
      541 ttaccgcttg ctgtgtcagt tgaaggtaga gttattctat ccatgaaatg gctataatct
      601 gtgaagaaga tcaacaaaaa acgatcatac cccagataat aaatcattaa ttatgaatat
      661 cgccatatta gatgctgttt tgttacttaa atgttgtaat ttcgtgtttt atatttgcgt
      721 taagaaatta aacctgccaa ataataatat tcggcgcagg tttaataata aattaatcac
      781 tatttaagcg aatttattga ataacaattc aaagcataaa ccgataccca ataccggttt
      841 cagtaatgaa gtggcgaggc cgggccggat cctgttccag tttttgtcgc agatgtccca
      901 tataaatacg caaatagtga ctgtgttcga ccgcgtttgg cccccacacc tggttaagga
      961 gctggcgctg ggtgagtact tttccggcat tgttgagcag caccgctagc aggcggaact
     1021 caattggtgt gagatgcacc tcttcctcac cccggtgaat cacgcgggcg gctaaatcga
     1081 cggtaacatc ggaaaatttc accagcggat cgggcgcggc ggtggcggag tggcggcgta
     1141 aagcgacgcg cagacgggcc tgcaattcgc caatgccaaa cggcttactc agataatcat
     1201 ccgctccggc atccagcgcg gcgatcttgt cgctctcttc gctgcgtgcg gaaagcacaa
     1261 tcaccggcac cgggctccac tggcgcaggt cgcggataaa ctcaatccca tcaccatcgg
     1321 gcaggccgag atcgagaata atcaaatctg gcttacgggt tgccgcttcc agcaagccgc
     1381 gttgcagagt ttcggcctca aagacgcgca tcccgtcgcc ctccagcgcc gtgcgcagaa
     1441 agctacgaat agcctgttca tcttcaacaa tcagaacgtt tgtcacatat cctcatgaaa
     1501 gtcttcaagt tcaggagcgg tttgctgggg aagtgtaaca cgaaaacagg caccaccttc
     1561 cggtcggttg aacgcggtaa tggtgccgcc gtgtacatcc acaatcgccc gacaaattgc
     1621 caacccaagc ccgacacccg gcaccgccga ctctttattc ccgcgagcaa acttatcaaa
     1681 tatcgtctgc tcctggcctg gcggaagacc ggggccgtta tcccagacat ccagttgtag
     1741 attttcgccc tcaacgtggg catcgatacc aatttcggcc tgcgcacccg catatttcac
     1801 cgcgttctcc agcagattaa tcagcacccg ttcaaagagt ggcccgtcaa cgtggattaa
     1861 ggtcagcggt tctggcagag aaagattgat gggcgacgat aaacccggtt ccagcatttg
     1921 cagcgcgctg ccgactactt cttccagcgt taaccactct ttcttcaaat taaagccgcc
     1981 ggactgaatt cgcgccatat ccagtagatt attcaccagt cgggtagtgt tcagcacatg
     2041 ctgacgaatc tcgctggcct ggcgggcgtg gggtgatcct tcgcttgcca gatcgagcgt
     2101 taagatttct gcctgaccaa acagcaccgt aagcggcgtg cgtaaatcat gcgaaagcgc
     2161 tgccagcagg gcgttgcgga tctgttcacg ttcgcttgcc atccgcgcct gttcttcgct
     2221 ggcggttagc gtcagccgct caagggcatt ggcgactaac agcgtaaaag tctccagcag
     2281 gcgctgctgt tccgggatca tcaactggcg cagattcccc ggctccacca ccaccagccg
     2341 taggttttct cgccgctttt tagcggcaaa atctggtacg gtacaccggg taacgtgtcg
     2401 gtgcccgcgc ccgcaggcag gcctttatca aaactccact gcgcgatggc atcgtcccat
     2461 ggcgtcattc cttgcggatg tgttaacggc tgcaatttac cgttgtcatc gggcaacaac
     2521 acctgactgc gggcatgaaa cgtagaggca ataaattgtt cgctggtggc ggcgatgtcc
     2581 tgcggactgc ggcccaccgc cagagctttc gacatttcat ataagtgccg tgtgcgttgc
     2641 tcgcggtaac gggctacccg cgcctgataa cgcacgccag cagtaaggtt cccgatcacc
     2701 agcccgacgg ttaacatcac cgcgaaggtc agcagatatt gcacatcaga gacggcgagc
     2761 gtgccgcgtg gggcgataaa aaagagatcg aaactcacca cattaatgac ggtggcaacc
     2821 accgaaggcc agcgtccata aaatagcgcc accaccacgc caagcagata cagcatcacc
     2881 aggttggcgg catcaaacgc catcagccac tgcatggcaa ttaaggtgat aacggcgcat
     2941 aacgcggcgg caaccacgca tccctgaatc tgtacccgcc acttgtcttt aaatgagcgg
     3001 ctatccggcg cgttgttaat cgtgcgggcg ggtggttcat caagcgcgac cagcacctga
     3061 tcgagatcgg gggcgatgcg cgccagtcgg tcagcaaatg tttcccgacg ccaccagcgg
     3121 cgcgaggctg ggcgaccgag aataatcttg ccgagattat gttcacgggc ataacgcact
     3181 accgctttct cttctgctgg atcagaaagt gttgccgtct ccgcgcccag ctcctgcgcc
     3241 agacgtaagg cgctgagaat tgcccgacgt tttttctccg gtaagcgatg cagggcaggg
     3301 ggttcaacat acaccgcgtg ccagacgctc cccagccgtg acgccagccg cgccgccgcg
     3361 cggaccagtt ttcgctgccg gtgttatggc cgatgcataa aaggatcgcg tcgcgcgtgt
     3421 gccagacttt ctcttcgcca ggatgccccc gccaggcgcg catttgctca tcaacgcgat
     3481 cggcagtacg gcgcagtgcc agttcgcgca gggcgatcag attgcctttg cggaaaaaat
     3541 gttcaatggc gcgctccgcc tgcccggcaa tatagacgtt gccttctttc agccgctggc
     3601 gcagatcgtc cgggggcaag tccaccagca ccacgtcgtc ggcggcatcg aaaaaaggat
     3661 cgggcacggt ttcccgtacc tgaattccgg tgacgccgct gaccacatca ttcagacttt
     3721 ccagatgctg aacgttgacg gttgtgaaaa catcaatgcc agcttccagc agttcttcga
     3781 tatcctgcca gcgtttagga tgacgagaac ctggcgcatt actgtgcgcc agttcgtcca
     3841 ttaagatcag cgccggacgg cgggcgaggg cggcatcgag atcaaactcg ctgatatgcc
     3901 gcccacggta cgcctggcgt ttcagaggca gaatagccag cccttcaagc atggcggcgg
     3961 tatctttccg cccatgggtt tctaccacac caaccacaat atccagccct tgcgcccgca
     4021 gtcgctgggc ttctgccagc atcgcccagg tcttcccgac gcctgcacag gcactgaaga
     4081 aaactttcag cttcccccga tgcggcgcgg cagtttgttc cagcagacgg tcggggtcgg
     4141 gacgtaaggg ttcgttattc atcaagctta tccagtgcca gattgagttc gacaatgttg
     4201 acgaccggct ggccgatata tttcaccagc ggttgttggc tgtattttgc gatcagctgc
     4261 gtgagctgtt caacgctgag attacgcgct tttgcgatgc gtgggatctg ccaggccacc
     4321 gcttgcgggg tgatgttatt gtccagtccg cttgccgacg ccgtcaccag ttcaaccgga
     4381 acgttcgtgc tggcgtccgg gttagcggcc cgtaatgcgg caacgcgtgc ggctatctgt
     4441 ttatccagct ccgggttact gactgccaga ttgctcccgc cagaagcctg tggattatag
     4501 ggcatttctg ctgttgccga cgggcgacca tgaaaatagc cgttgccggt aaaattttgc
     4561 ccgattaatg ccgaaccgcg caccgtatca ccttcacgaa tcaacgaacc gttggcctgc
     4621 cagggatacc accattgccc cagtgcggtg gtcagcagcg ggtaaacgcc gccagtaatc
     4681 aataacagaa agagaaatgt cgataatgcc ggacgtaatc cactcatggt aaacctcaca
     4741 ccagactaca aacggttagc agtaaatcaa tgactttgat accaataaac ggcaccagca
     4801 gcccacccag accgtaaatc cataagttac ggcgcaacat ggcagaagcg gtaagcggtt
     4861 tataactcac gccttttaac gccagaggaa tcaaaaagac gataatcaag gcgttgaaaa
     4921 tgaccgcact gagaatggcg gagtcgggcg aatgcaggcg catgatgttc agcgcattta
     4981 actgcggata tgttgccgcg aacgccgccg gaataatggt gaagtatttc gccacatcgt
     5041 tggcaatgct gaaggtggtc agcgagccac gggtcatcag catctgtttg ccaatgtgca
     5101 ccacctcgat caacttggtc gggttagagt cgagatccac catattgccc gcctctttcg
     5161 ccgcctgggt gccggagttc atcgccaccg cgacatctgc ctgcgccagc gccggagcat
     5221 cgttggtgct gtcgccggtc atcgctacca aacgaccttc cgcctgatac tgacggatca
     5281 atgccagctt ggcctccggt gtcgcttcgg cgagaaaatc atcgacaccc gcttccgcag
     5341 ctatcgcggc ggcagtcaga cggttatcgc cggtaatcat caccgtttta atgcccattt
     5401 gcgcagctgg gcgaagcgct ctttaatacc gcctttgacg atatctttca gcgcaataac
     5461 gcccagcaca cgagaacctt ccaccaccac cagcggcgtg gctccttgac gcgcaacctg
     5521 atcgactttt tgatcaacat cggcaggg
//
LOCUS       DADLTF010000207         5537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.209, whole genome shotgun sequence.
ACCESSION   DADLTF010000207 DADLTF010000000
VERSION     DADLTF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.209"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="JN749_003856"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="JN749_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HAY7370081.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="JN749_003857"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="JN749_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HAY7370082.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..>5537)
                     /locus_tag="JN749_003858"
     CDS             complement(2659..>5537)
                     /locus_tag="JN749_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HAY7370083.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGSSYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         1462 a         1288 c         1303 g         1484 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaaaaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg acttatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggctgt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag tccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccaactc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagctg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccac
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaag
//
LOCUS       DADLTF010000208         5531 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.210, whole genome shotgun sequence.
ACCESSION   DADLTF010000208 DADLTF010000000
VERSION     DADLTF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.210"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            100..1008
                     /locus_tag="JN749_003859"
     CDS             100..1008
                     /locus_tag="JN749_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAY7370084.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFY
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAADKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPETMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JN749_003860"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="JN749_003860"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAY7370085.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /locus_tag="JN749_003861"
     CDS             3323..4081
                     /locus_tag="JN749_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HAY7370086.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JN749_003862"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="JN749_003862"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HAY7370087.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="JN749_003863"
     CDS             complement(5343..>5531)
                     /locus_tag="JN749_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370088.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1325 a         1482 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tctacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag acaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gaaaccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DADLTF010000209         5466 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.211, whole genome shotgun sequence.
ACCESSION   DADLTF010000209 DADLTF010000000
VERSION     DADLTF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.211"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(296..580)
                     /locus_tag="JN749_003864"
     CDS             complement(296..580)
                     /locus_tag="JN749_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAY7370089.1"
                     /translation="MLDMAGLRADILSPVKETGTLLGVVSSQAAELCGLKAGTPVVVG
                     GGDMQLGCLGLGVVRPAQTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
     gene            614..1420
                     /locus_tag="JN749_003865"
                     /pseudo
     CDS             614..1420
                     /locus_tag="JN749_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
     gene            1432..2455
                     /gene="lsrB"
                     /locus_tag="JN749_003866"
                     /pseudo
     CDS             1432..2455
                     /gene="lsrB"
                     /locus_tag="JN749_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            2482..3357
                     /gene="lsrF"
                     /locus_tag="JN749_003867"
     CDS             2482..3357
                     /gene="lsrF"
                     /locus_tag="JN749_003867"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HAY7370090.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PGVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            3381..3671
                     /gene="lsrG"
                     /locus_tag="JN749_003868"
     CDS             3381..3671
                     /gene="lsrG"
                     /locus_tag="JN749_003868"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HAY7370091.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            3710..4468
                     /gene="tam"
                     /locus_tag="JN749_003869"
     CDS             3710..4468
                     /gene="tam"
                     /locus_tag="JN749_003869"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HAY7370092.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(4472..5386)
                     /locus_tag="JN749_003870"
     CDS             complement(4472..5386)
                     /locus_tag="JN749_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370093.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNELPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         1379 a         1306 c         1457 g         1324 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt
      661 actgtttcaa agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc
      721 attgtgcggg ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat
      781 tacgcttggc acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg
      841 agcgacgggg tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct
      901 ggatgtgctg ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg
      961 gctctggctg cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg
     1021 cgtggcgctt tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac
     1081 ggggctggcg tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc
     1141 cgatctcggt gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa
     1201 tatttatggt ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata
     1261 tttgcagcaa ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg
     1321 tgcgctgctt atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa
     1381 agagtggctg gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt
     1441 catcgcttta agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat
     1501 gtgcaggccg cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc
     1561 agcggtggca acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac
     1621 gggccgacag aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa
     1681 ggttataacg ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa
     1741 acgcgccatg caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg
     1801 ccgctcttac tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat
     1861 ggcggcgcgt caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac
     1921 cgttacggac caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc
     1981 aggctgggaa attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac
     2041 cgcagaagga atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa
     2101 cgccctgccc gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt
     2161 cggattcagt acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt
     2221 tggactgtgg gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgcattatt
     2281 gaaaaaagga tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga
     2341 agtctcgcca aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact
     2401 gttaccggag cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc
     2461 attacttaac cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt
     2521 cgtaccgatc aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctggat
     2581 tggggaatgc agtcacgctt atcgtggata tttaatccga aaacgggtaa aactgtgatg
     2641 ctggcctttg accatggtta ttttcagggg ccgactaccg gacttgaacg cattgatata
     2701 aatatcgccc cgctgtttga acatgccgat gtattaatgt gtacgcgcgg cattttgcgc
     2761 agcgtagttc ccccggcgac caataagccg ggggtactgc gggcgtcagg tgcgaactct
     2821 attctggcgg aattaagtaa tgaagccgtg gcgttatcga tggatgatgc cgtgcgcctg
     2881 aacagttgcg cggtggcggc gcaggtttat atcggcagcg aatatgaaca tcagtcgatc
     2941 aaaaatatta ttcagctggt cgatgccgga atgaaagtgg gaatgccgac catggccgtg
     3001 actggcgtgg gcaaagatat ggtgcgcgat cagcgttatt tctcgctcgc gactcgaatc
     3061 gccgctgaaa tgggggcgca aattatcaaa acctattatg tcgaaaaagg ttttgaacgg
     3121 attgttgccg gatgtccggt acccattgtt attgctggcg gtaaaaaatt accggagcgc
     3181 gaggcgctgg aaatgtgctg gcaggctatc gatcagggcg cttctggtgt ggatatgggg
     3241 cgtaatattt tccagtctga ccatccggtg gcgatgatga aagccgtaca ggcggtggtt
     3301 caccataacg aaacggctga tcgggcatat gaactctatc tgagtgaaaa acagtaactg
     3361 cggatttaag gagaagaatt atgcacgtca cactggttga aattaacgtt catgaagaca
     3421 aggttgacga gtttatcgaa gtttttcgcc agaaccacct gggctctgta caggaagaag
     3481 gtaatttgcg cttcgatgtc ttacaggacc cggaagtgaa ttcgcgcttt tatatttacg
     3541 aagcctataa agatgaagac acagtggcat tccataaaac cacgcctcac tacaaaacct
     3601 gtgtcgcgaa actggaatct ttaatgaccg ggccgcgtaa aaaacgtctg ttcaatgggt
     3661 tgatgccgtg aggctacaat tggggtaacg cgctgacggg agtaaaaaaa tgtctgactg
     3721 gaacccctct ttatatctgc acttttccgc tgaacgatcg cgtccggcgg tggagctgct
     3781 tgccagagtg ccgctggaga atgtcgaata tgttgccgat cttggctgtg gcccaggtaa
     3841 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
     3901 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
     3961 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
     4021 atggctgccc gaccactacg aattgtttcc tcacttggtt tctttactta atccgcaggg
     4081 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
     4141 agttgcttgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
     4201 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacttacta
     4261 tcaccagatg ccgtcgcacc aggcgattat cgattgggtg accgccactg gattacgtcc
     4321 gtggttacag gatctgaccg agagcgaaca gcagcttttt cttacgcgct accatcagat
     4381 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
     4441 gtttattgtt gcccgccgta cggagtaatt atcacgtcag ctggtaatga cgatcgggaa
     4501 gaatttttgc tggaatttct gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
     4561 tgcagatggc atccagcggt aactcattgt tttcagtacc gaacggatct tccagttctt
     4621 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
     4681 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
     4741 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtgta ggcaatgcgc tcacagcctg
     4801 ccagaaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
     4861 gctgtccatt gcggcgctga accgccaacc attctcccat tattaacaag atacggttag
     4921 ctggagagtt cgaagccagt acacgctgaa gatctccagt cttgagataa tgagccagca
     4981 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
     5041 gcctggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tctcgcagta
     5101 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
     5161 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
     5221 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaat ataacagcaa
     5281 tagaaaagag aaaattgagt agtaagcgcg aggatatctt ggataatact gagccgtgcc
     5341 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
     5401 gtggaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
     5461 ttacct
//
LOCUS       DADLTF010000210         5463 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.212, whole genome shotgun sequence.
ACCESSION   DADLTF010000210 DADLTF010000000
VERSION     DADLTF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.212"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            2..355
                     /locus_tag="JN749_003871"
     CDS             2..355
                     /locus_tag="JN749_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370094.1"
                     /translation="MASQPGASVARIAREHDINDNLLFKWLRLWQNEGRISRRLQVTT
                     SSDTGVELLPVEITPDEQKKTVAAIAPSLSTSTQTRVSASSCKVEFRHGNMTLENPSP
                     ELLTVLIRELSGRGR"
     gene            352..642
                     /gene="tnpB"
                     /locus_tag="JN749_003872"
     CDS             352..642
                     /gene="tnpB"
                     /locus_tag="JN749_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7370095.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAML"
     gene            complement(894..1637)
                     /locus_tag="JN749_003873"
     CDS             complement(894..1637)
                     /locus_tag="JN749_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005067636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAY7370096.1"
                     /translation="MVKILAVAQSHESIVSMDEINSILLSSSWFRALPDYLIEQMLSR
                     TSLYQYNDGEIIHYRGAEPLGLYAVITGSVKVSSISADGKECIFRYLSPGNWFGEIGM
                     IDKCPRTHDARATGKTTLLTIKPHDVEFLLKNYPILHRFLALLLCKVVRNAFTMLHDC
                     TLLSVSARLAKRLVSLSEGYGEPHERGTLINLYLTQDDLATIINTSRQTINKRLVEWE
                     KLGWIDARYGKIVIININALRQLYADEDE"
     gene            complement(1687..3054)
                     /locus_tag="JN749_003874"
     CDS             complement(1687..3054)
                     /locus_tag="JN749_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7370097.1"
                     /translation="MPSHTDQYLSLAIQKSIRRIIPMVILMFILAYLDRSNIGFAKQE
                     FQLDTSLSDTAYAFGAGIFFIGYALFEVPSNILLYRIGARVWLARIMVTWGLVSAAMM
                     FAHTETVFIFLRFLLGISEAGFFPGVILYLTFWFPQHIRARVTGYFLFGAPLAFIIGG
                     PLSGSLLSLEGSVLSFGLHGWQLMFLVEGLLASAVGIWVFFYLDDHPEKAKWLTPQEK
                     AALSQTLNKEEQVKLAHGPKGAWQALLDVRVLYLCLIWFTVQVCGYGIYFFLPTQIAT
                     LIGTKVGLLVGFVTAIPPLCAAIAVYYVPRISEKLQERRYVASITFMSGAIGIAVSGL
                     ADSIPLVAIVALCVAAAGHLAMQPLYWTFPSSYLGGAAAASGIALINSIGNLGGFVAP
                     NLRVWAEHTFNSAQAGLYLLAMVTATGSLMILTLRHFGIEKTPRTSSTSPNLPASGDN
                     LHHSD"
     gene            complement(3126..4238)
                     /locus_tag="JN749_003875"
                     /pseudo
     CDS             complement(3126..4238)
                     /locus_tag="JN749_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705119.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
     gene            4404..4885
                     /locus_tag="JN749_003876"
                     /pseudo
     CDS             4404..4885
                     /locus_tag="JN749_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
     gene            4946..5379
                     /locus_tag="JN749_003877"
                     /pseudo
     CDS             4946..5379
                     /locus_tag="JN749_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1526 a         1318 c         1322 g         1297 t
ORIGIN      
        1 actggcatca caaccaggag cctctgttgc ccgtattgcc cgggaacacg atatcaatga
       61 taacctcctg ttcaaatggc tcaggctctg gcagaacgaa gggcgtatat cacggcgtct
      121 tcaggtaacg acctcttctg acactggcgt tgaattatta cctgtagaaa taacgccgga
      181 tgagcagaaa aaaactgtgg cggccattgc gccgtcttta tccacttcca ctcagaccag
      241 agtcagtgcc agttcctgca aggtggagtt ccgtcacggt aacatgacgc tggaaaatcc
      301 atcgccagag ctgctcacgg tgttgatccg cgaactgagc gggaggggaa gatgatttca
      361 ctcccatcag gtacccgtat ctggctcgtt gccggcgtta ccgatatgcg taaatccttc
      421 aacggactgg gagaacaggt acaacatgtg ctgaatgata atcccttctc cggccacctg
      481 tttatcttcc gtggccgacg gggtgacacc gttaaaattc tttgggctga tgctgatggt
      541 ctgtgcctgt tcaccaaacg ccttgaggaa ggtcagttta tctggcctgc ggtgcgtgac
      601 ggtaagatat ccattacccg ctcgcaactg gcaatgctgt aaacgttaac ggagcgccgt
      661 attgacgctt acgaactgtg tgatccgaca tgccattagg aaacgtaagg cgttcccgat
      721 gggcgagtca gtaaaagtga aatggctggc atcctggtcg cgtcgcagaa atggacaatg
      781 ccgcagaggg actggcgaat agcaatttat tatcgaattt gatgaccgcc tggacagtca
      841 cttttaagaa aaagcatgta tacagaatgg taaacaggct catccacagc caattactca
      901 tcttcatcag catataactg cctgagtgca tttatattga tgatcacgat tttgccatat
      961 ctggcgtcta tccatcccaa tttttcccat tcaaccagcc gtttgttgat cgtttgtcgg
     1021 ctcgtattga ttatcgttgc cagatcgtcc tgcgtaagat aaagattgat taatgtccct
     1081 cgctcgtgtg gttcgccgta cccttccgac agactcacta aacgcttcgc caagcgtgca
     1141 gaaacagata atagcgtaca gtcatgtaac atcgtaaagg catttcgaac gactttgcaa
     1201 agcagcaacg ccagaaaacg atggagaatt ggataatttt tcagaagaaa ttcaacatca
     1261 tgaggcttga tcgttaacag tgtagtttta cccgtcgctc tggcatcgtg tgtgcgcgga
     1321 catttatcaa tcatacctat ttcaccaaac cagttccccg gcgaaagata gcggaagatg
     1381 cactctttac cgtcagcaga aattgagctc actttaacgg agccagtaat gactgcatat
     1441 aatccgagcg gctcagcacc acgatagtga ataatctctc catcgttgta ttgatacagg
     1501 ctggtcctgg acaacatctg ttcaataaga taatcaggta gcgcccgaaa ccaactacta
     1561 ctaagaagga tactattgat ctcgtccata gaaactatcg attcatgtga ttgtgctacg
     1621 gccaatatct taaccattgt tgttatcctg atcttaatca tgaccgtagc aatgtagcat
     1681 ttcctgtcaa tcagagtgat gcagattatc cccgctggca gggagattcg gagatgtgga
     1741 tgatgtacgg ggcgtttttt caatcccgaa gtgacgtaat gtcagaatca tcaggctacc
     1801 agtagctgtt accattgcca gtaagtaaag tcctgcctgt gcggaattaa aagtatgttc
     1861 cgcccagacg cgaagattcg gcgctacaaa cccccccaga ttgcctatcg agttaattaa
     1921 cgcaatgccg gaagcagctg ctgcaccgcc gagataagac gatggaaatg tccagtacag
     1981 tggctgcatc gccagatgtc ctgcagcggc aacgcacagc gcgacaatag cgaccagtgg
     2041 aatactatcg gcaaggccag atacggcaat gcctattgca ccggacataa atgtgattga
     2101 tgcaacgtaa cgacgttcct gcagtttttc cgatatacgt ggtacgtaat acactgcgat
     2161 ggcagcgcac aaaggtggaa tagcggtgac gaagccaact aataaaccaa cttttgtccc
     2221 tattaaggtg gcgatttgtg ttggcagaaa gaagtaaatt ccataaccac atacctgcac
     2281 cgtaaaccag atcaggcata aatagaggac gcgtacatcc agcagtgctt gccatgcgcc
     2341 ttttggacca tgtgcaagtt tcacctgctc ttccttattt agagtctggc taagcgccgc
     2401 tttctcttgt ggagttaacc atttggcttt ctctgggtga tcgtcaagat agaagaacac
     2461 ccagatccca acggcagagg ccagtaaccc ctcaacgagg aacatgagtt gccagccatg
     2521 gagaccaaac gacagtaccg atccttcaag cgagagtagc gagccggaaa gcggaccgcc
     2581 gatgataaaa gctagtgggg caccaaacag gaaatagccg gtgacgcgtg cgcggatgtg
     2641 ctgggggaac cagaatgtaa gataaagaat aactccagga aagaaacctg cttcactgat
     2701 gccaagcaag aagcgcagga aaataaatac ggtctcagtg tgggcgaaca tcattgcagc
     2761 ggaaaccagc ccccaggtca ccatgatacg ggccagccag acacgggcgc cgatgcgata
     2821 aagcagaata ttactgggca cctcgaacag ggcataacca ataaagaaga tcccggcgcc
     2881 gaaagcatag gcggtgtcac tcagactggt atccagttga aattcttgtt tagcaaagcc
     2941 gatattggag cgatccagat aggccagaat aaacattagt atgaccatcg gaatgatgcg
     3001 acgtatggat ttttgaattg caagtgataa atactgatcg gtatggctgg gcataaatca
     3061 cctcttagaa ttttgtcatt attattttgc cccggtcatt gcataaccgg gcgatacaag
     3121 tgttctcatc taacgctaaa gtgctcaaga gcttccgggc gtaaggtagt gccggcaccc
     3181 ggcgcggtgg gaagagagta atatccctct ttgacctcaa ccggatcgac aaagcagccc
     3241 agcgtccagg gaataaactc caacagagag caagagggat gtgccattac cagatgtaat
     3301 tgtgcctgca tcatatcacc atgatgtggt gtgaccggca gattgaacgc ctgaccgaga
     3361 tcggctattt cccatacggc ggtcagtcca ccgcagcgag tggcatcggg ctggagataa
     3421 tccaccgcac cggccagtac aaactcttta aaggaatcaa actgatagag taactcaccc
     3481 aacgcaatcg gcgtactgat atgcccggac agcgtgcggt gacttgcgat atcatcatgt
     3541 cacatcggct cttcaaacca ggtaatatcg aaatcagcca gcttggcacc gtactgcttg
     3601 gcagtgctga tgtcccattt tgcattcgcg tcgaccatca gattgacgtc atcgcctatc
     3661 gcttttctga cggcttcgat tcgtttgaga tcttctcgcg gatcgggctt gcctatcttc
     3721 atcttgatgg cggaaaatcc ctcctcaaaa atcatcttct tgcagtcatc cactaactcc
     3781 gccgtgttac gaaccagcca gccgcagtcg gtgttgtagg caagaacacg ggaatgttga
     3841 gaaccgccaa agactttcca caaaggttgc tccaccattt tggctttcag gtcccacagg
     3901 gcgatatcga ttgctgaaag cgccatttgc attaacccac cgcggcccac ccagatattg
     3961 gtcgagctgc gtgacatctt acggtggaga tagcgaactt cactggcgtc ttctccaatc
     4021 aacatcgggc cgaaggcatc tttaataatg gtggtgataa gacggtcggt agtaatgtct
     4081 gcatgggttc cggtataacc gtaacctaca aggccggtat ctgtctcgat cattacgccc
     4141 ggcattcccc aatgagtaat gctgtgtgtg gagtcaccga taatattttt agttaccggt
     4201 acatgcataa taaaggtagt gagtttagtg attttcattg cagtttcctg tgtctgtata
     4261 tcttgattga tgggcgcaat gtagcaaccg gccttcagca gggctgtcaa atggttgaca
     4321 catcatcaga agagagaagg aataattgta tttttttgaa gtagatctcg aatttggacc
     4381 acacccagtc gacaagtttt catataggga actgggccat gaccggagct tttcaggatg
     4441 agatctctgt acgcatcatg aagaccatgc ggctaaagtg atcctggagt acagcaaact
     4501 ggaaaaaatc gaaaagtatt acgaaaaata cgcaaacgca ggcaccaatg agtggcacat
     4561 cgatgaaagt aaaaaagcta ccgcaaccat gaatgtagtt ctgggtgatg aagataggtt
     4621 aaaatccctc gcggaagatt ttgccagaca ttatgaaaaa cgcgtagccg aaggtttcac
     4681 cgtaaaaggc aagaccatgt ttgtgccagc cgtgaaattg cctagggttc taccgtcacc
     4741 ttgaagctat tcgcactgcc tggttttaag tgaaacaagt acccgatggc atcgtcctga
     4801 ctgaacggga gcagaaaaat taccgccttc tgaaatggtg aaaatggtca tgacgcgtgg
     4861 taaagatgat gacgaggcgc tttatagatt tgtgtaattg cctgattttg atatgttcaa
     4921 ttcaacatca aatgaaggtt aatttatgga cgaaaaatag ttacagactc tggctaacga
     4981 actggccaaa aacctcaaaa cccctgaaga cctcagccag tttgaccgtc tactgaaaaa
     5041 gatcagcgtt gaagccgctc tcaacgcaga gatgacacac catcctgggt atggaaaaat
     5101 cagtccagac caggagctaa ctcccgcaac ggttattcca caaagaccgt tatcacaggc
     5161 gacggtccac tggaactgcg tactccgcgc gatcgtgacg gtagcttcga accacaacag
     5221 gtgaagaaaa atcagccccg tattaccagg atggataacc agatcctctc gttgtatgcc
     5281 aaagggataa ccacccgtga gatagccgcc gcgttcaaag acctgtatga cgctgatgtt
     5341 tcaccggcac tgatatcaaa ggtcactgat gccgtgatgg tgaaccgccc cggaaatcct
     5401 ggagactaaa ctccctgaga aagaggtaaa caggatgact aaaaatacac gttttccccc
     5461 gaa
//
LOCUS       DADLTF010000211         5425 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.213, whole genome shotgun sequence.
ACCESSION   DADLTF010000211 DADLTF010000000
VERSION     DADLTF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.213"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..87)
                     /locus_tag="JN749_003878"
     CDS             complement(<1..87)
                     /locus_tag="JN749_003878"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7370098.1"
                     /translation="MALESDERMLPPNIRGYAPQITGIAETNA"
     gene            complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JN749_003879"
                     /gene_synonym="virG"
     CDS             complement(249..3557)
                     /gene="icsA"
                     /locus_tag="JN749_003879"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HAY7370099.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGSS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4086..5288
                     /gene="virA"
                     /locus_tag="JN749_003880"
     CDS             4086..5288
                     /gene="virA"
                     /locus_tag="JN749_003880"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HAY7370100.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1683 a         1223 c          899 g         1620 t
ORIGIN      
        1 tgcattagtt tctgcaatac cagtaatctg aggcgcataa ccacgaatat ttggcggtag
       61 cattcgttca tcgctttcta atgcaattcc tgtgtaacgc caagcaacag cgcattctgg
      121 cgcatgatct ggtaaacacg tttggcattg atcgcaggca taccatcatg tgcacaaaaa
      181 cccaccgagc ttttacacgg tgggtcccag agaaatgcag gacatcaaca cgccctgcat
      241 ttttattatc agaaggtata tttcacaccc aaaatacctt gggtatctct gtaactgtta
      301 ttacccaatt gctgagatac gtttccccag atgcttaggt tatctgcaag acgccccccc
      361 acaccagttt tgagttcaat cacattacgg ttgcctatct ggtgatttga catgccattc
      421 ataattacac catattgctt tgagctccat ttccaattca cttccaaaaa tggttcgaac
      481 ttacgaagag tctccttatc gatacaactc tgactaatta aggatgtacg caatcccaat
      541 tttgcttgaa tattattgac cccagaggat gtgaccatag tgccattgtg ttcaatatag
      601 tcgtttgctt ttaccccatg cctagttatc tggacttgtg gattgagata caatgcgttg
      661 tcaatattat tatgagcagt ccagcgtatg gttgggtaga tatagccagc ttccaaagct
      721 cctgttattc ctttgctgga atatttctca ccagttaacc catctccttt gactgtatta
      781 ttaaaccaac tgtactgcaa ccaagcatcc atatatagac ctgttcgttc tttttcatcc
      841 tgaaaccaag aactgtacaa accggcagta taaccgctca cagttcctcg tgaactataa
      901 ctagtatact tattatgagt tttgctatcc tgatatccat aagctcccat aatacccaca
      961 tgcaagccat catgatcagt gaagtttgtc ttcaaaatat ctccaccaat ctgataaacc
     1021 atcctgttag ctgtggtttt taattgtcta tcagctaaat tatgctcatt atgtccacca
     1081 acagtacgaa tccacatggt ggtttcgctg agctgacctg tagtaggatc aacaatagtg
     1141 ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt tcattgctat caggttagca
     1201 atataactgc cagcttcagg gcgccatgtg tgaatactac cttcacccaa attaagtgaa
     1261 gatacaagct gtgaactagc tcggcgacta ctcatttgag tagactcttg attgctcatc
     1321 tgttttgatt cttgattatc catctgacta gttagatacc acttattggt attcagtcca
     1381 gatacagtgc cctgtttcag gcgatagtca taactaccag caacaatacg gcctttctga
     1441 ataaaagcat tcttatcaga agaatctgtc gaaataattt gaacaccttc aagagtttta
     1501 tctccagagc ctccctcatt tgtcactgca acataggtaa taccagacgc atggcctgca
     1561 atttccagcc ggtcagtttt actgttgtca ttgccgagaa ccgcgcccaa agataatatt
     1621 ccgccctttc catgccaatt acctttctga acataggtct gaccgacatt tgacgaacta
     1681 ttattgagaa taacatgacc gtttttctcc agagtcatgt tacctgtgac aatgacgggg
     1741 tcaggcaaaa aaggggcatt aatattattg attagcacag tcccactatt taataaagtg
     1801 ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac ctttattaac aataacacca
     1861 gcggtacgtg tcatagcttc aactgtcccc atttttagaa tgcctgcatt gatgttggtg
     1921 aaaccagagt aggtattttt ttccgccaaa atgagagttc cctcccctga ttttgttagg
     1981 gattttccgt cccagctatt tatattatga tggctgagat tatctgccag aatagtacta
     2041 acagtaagtt cagcgttttc tttcagattg aactctccat gactgtcgcc atctgtatca
     2101 ttccagctca aaccgtagat caatttattc tggtcactga tatagctttt tgtatagtca
     2161 atagcaccag tagtgagtaa cggattgatg atagatatat tagaaacact ggaacctgtt
     2221 atccctttat ctgcacttag cagtttatag tccttctgcg cccagttatt tataatagct
     2281 tccactttta cattgttgcc aaatgacata tttccagaag cttccagact actgattcca
     2341 gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac ctgaaacagt gaatttgtta
     2401 cctgatagtg aaacagttgt acaatcacca gcacataaat caccttcaat agttggtgtg
     2461 gtcccagtgt aatcattgtt gataattttc agaatggatg aattgttaac ctgtacatca
     2521 ccagtaatga cagaaccttc atggagtatt aatgaattgt ttccaccagt gatgtggata
     2581 gcattacctg ctacattttg tgttccatca tcttctttac ctgaaattat atatccgcta
     2641 ttagcaatgg tcatattgct accatttata gcatcccccc aatgtccacc attaccgcct
     2701 aaaatagctc cattgttgat tacagacagg ttatctcctg tgatagcatt accaccgtag
     2761 ccatcataac cataaccgta agcagcacct ccgttgcctc cagcgaacgt gcccttattg
     2821 attacagaga ggctgcttcc tgtgatggca tcacctccat taccgccatc accctcacca
     2881 taattgttac ctccattacc accggaaata gtccctttgt tgattatggg tagattgact
     2941 cctgtgacgg catcacctcc attcccccca tcactatcac tataactatc gccaccatgt
     3001 ccacctgaaa taatttctcc atttattata aacagattgt ctcctgtaac agcctcacca
     3061 ccatcaccgt tatggtcagc accgctaccg ccgctaccac caagaatcat gccttgattg
     3121 attatagaca agtcagatcc ggtaatagag tcaccaccat taccgccaca actattacca
     3181 tcattattat caccagcacc accatgacct gcgctaatca taatatcaga gttaatccgt
     3241 agcttagttc tagatgcatg agaggggttg gaagaagtta tataccacgc atccattcca
     3301 tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg ttaataattt ttcataattg
     3361 tcctctgaaa aatgaagttc ttgagtaccc gaaagaggag tagcaaaagc tattggcccc
     3421 ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag acaatttgca tgttttttcc
     3481 tttaccgtag gtaattctcc ggcccccccc tgtgaacatt gggtcatatt acaaaaaaat
     3541 ttgtgaattt gattcatgca ctatattatc agtaagtggt tgataaaccc ctgaaaagat
     3601 tccgttttat ccggaataaa gggacgatat atgcaaaaac atattaaaca aagcctcaac
     3661 caaaacatga gagctgtgtt atatatcaat gcaacgggag aaatttacct gtgtcttgct
     3721 ttgaaaaaaa gaggggaaaa gctatatgtt cgataataaa ttaataattt tactatgata
     3781 gtctgcttca gaccgcaact tttcagtcct ttctgtacgc ttgcccaaag tttaaccact
     3841 ttgtttttct atacacccaa tggttctatc tgccccttga tgttaatccc caaagccaat
     3901 aacatgaatg tattcatatc acaactttcc taccagactc tccaatgcaa taactgcata
     3961 attgttttta gtggaaatgt atcgttttct taaagagaag aataacattc cattttatta
     4021 tgttcccaat ttaattatat taatatatat tggcattaat aggaaaatac atcaggagaa
     4081 atcaaatgca gacatcaaac ataactaacc atgaaagaaa tgactcatca tggatgtcaa
     4141 ctgtcaaatc aactacagaa gtaagttgga ataaattgag tttttgtgac gttttactga
     4201 aaataataac tttcggcata tattctccac acgaaactct agccgaaaaa tatagcgaaa
     4261 aaaaactaat ggatagtttc tcaccatcac tatcacaaga taaaatggac ggagagtttg
     4321 cccacgcaaa catagatggc atttcaatac ggctatgcct taacaaaggc atatgttcgg
     4381 ttttttatct tgatggagat aaaatacaat ccactcaact ttcatcaaaa gaatataata
     4441 acttgttaag ttcactcccc cccaaacaat tcaacttagg aaaagtacat actattacag
     4501 ctccggtatc agggaatttt aaaactcaca agcctgcacc agaagttatt gaaacagcca
     4561 ttaattgctg tacatcgata ataccaaacg atgattactt ccacgttaaa gacactgatt
     4621 ttaatagtgt ttggcatgat atttacagag atattcgagc atctgacagc aatagtacaa
     4681 aaatatattt caacaatatt gaaataccac taaaattaat tgcagatcta attaatgagt
     4741 taggaattaa tgaattcatt gatagcaaaa aagagcttca aatgctaagt tacaaccaag
     4801 taaataaaat aatcaattca aacttccccc aacaagacct atgttttcaa acagaaaaac
     4861 ttttgtttac ttcactcttt caagaccctg catttatatc tgcactaaca tctgcattct
     4921 ggcaatctct tcacatcacg tcttcctctg tggaacacat atatgcccaa attatgtccg
     4981 aaaacataga aaacaggctt aatttcatgc ctgaacaacg agttattaac aattgtggac
     5041 atattataaa aatcaacgca gttgtcccta aaaacgacac tgcaatctct gcctctgggg
     5101 ggagggctta cgaagttagc tcatcaatat taccatcgca cataacatgt aatggtgttg
     5161 gtattaataa aatagaaaca agctatctcg ttcatgctgg aacacttcca agcagtgaag
     5221 ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc atttgccatc atatctcctg
     5281 atgtttaagc caatattata ttggctgcca gactgtaaaa aaacgttctt cagaacagct
     5341 ctataaacta ttaaaccaaa gctaaaagga tggggaccaa tagaatctca gcccatcctt
     5401 tttatatcat tatgaaccgc cccgg
//
LOCUS       DADLTF010000212         5410 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.214, whole genome shotgun sequence.
ACCESSION   DADLTF010000212 DADLTF010000000
VERSION     DADLTF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.214"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(142..375)
                     /locus_tag="JN749_003881"
     CDS             complement(142..375)
                     /locus_tag="JN749_003881"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370101.1"
                     /translation="MSKEYTLSVPPFSLNTLGVDMYNNSSYIRKSYIDIPMYNRNLCD
                     FHDLTYSIKSATAILNGKQRDLLKEEHIQVQDK"
     gene            complement(372..1643)
                     /locus_tag="JN749_003882"
     CDS             complement(372..1643)
                     /locus_tag="JN749_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7370102.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKAALWLSWMYYNGLGETLDCDKV
                     WDYYEKGAGKYSMKFNKDEYLTRCNQDQKTRETQGDTLPYITLKHYGTYGFNANEKPK
                     ICDVSIEINADKISSITNLQLTLELKGGGRE"
     gene            2037..3407
                     /locus_tag="JN749_003883"
     CDS             2037..3407
                     /locus_tag="JN749_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7370103.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3589..5410
                     /locus_tag="JN749_003884"
                     /pseudo
     CDS             3589..5410
                     /locus_tag="JN749_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
BASE COUNT         1671 a         1127 c         1044 g         1568 t
ORIGIN      
        1 agtgtattta taacatcggg agcagattta ccaccgtata tgctgtcata caattcatgc
       61 gctttcttac ttgccaccac gcctgatgaa actatcagca ttaaagaaaa taatattcta
      121 ttaggcaaca taaaatatct cttatttatc ttgtacctga atatgctctt cttttaataa
      181 atcacgttgt tttccattca gtatcgctgt cgcagattta atagaataag tcaaatcatg
      241 aaaatcgcat aaattacgat tatacattgg gatgtctata tagctttttc ttatataact
      301 ggaattatta tacatatcca caccgagcgt attcaaacta aaaggaggga cagaaagagt
      361 atattcttta ctcactccct ccccccccct tttagctcaa gcgtcaactg caaattagtt
      421 atactactaa ttttatctgc attaatttca atactgacat cgcatatttt gggtttttca
      481 tttgcgttaa agccataggt cccgtaatgt tttaacgtga tgtaaggtaa agtatcaccc
      541 tgtgtttctc gggttttctg atcttgatta caacgggtta aatattcatc cttattgaat
      601 ttcattgaat atttccccgc acctttttcg taataatccc ataccttatc acaatcaagc
      661 gtttcaccta atccgttgta atacatccaa cttaaccaca atgcggcttt ttttgcccct
      721 tgctgttcag ccttgtcaaa aagcatgctc gctttagaat aatcaacctc accggtcaaa
      781 ccagaatagt atatattccc cagggccact aatgcttttt cgtcattctt atttgcagct
      841 aattcgtacc attttttggc ttcgacagca tcaatcttta aaccagcact gccattttcg
      901 taggtatcgc ccatggtata ctgcgccatc ggataacctt tttctgccgc agtacgaaac
      961 cattgcagtg ctttctttat ggtgtctaaa gaacgtctat catcagaagg tgaaaaatca
     1021 tcgagataaa gatttccgac aatataatcc atttcggggt cgccttcctg cgcccataga
     1081 gtcgccagac gcagaatttc aggtttactg aaaccaattc gcccccaaaa aagcaaatca
     1141 ttctcgggtg cctgaccgac ataacgactg acaaattcaa ttaaactcgc ccgtttcgtt
     1201 ttgtctgtct cagccccgga tttcaattca taggcctgat tgattatttt attcatcaaa
     1261 tcaggcggaa tattaagatc gtcagttttc gacttactgg catacttctg tgctcgtgtc
     1321 acatctaggg tgacgccttt accatattga aagaaataac gtgcattata acaaccgtat
     1381 tcgcccccct ggttacaggc tttctcatac cactgaatcg cctttgggat gtcaacagat
     1441 actgtcactc cgtgttcata aataaatccc agtagcacta gcgcctgcgt gttattggta
     1501 tcagctaatt ttttcagttc ttttaacgcc gtctgatctt tattgacaat acgttcatac
     1561 agttgatcaa tatctgttga agcatgacaa aaagaagcca gagataaacc caatgataat
     1621 aaaataactt taccagacca catacattcc tccttgatat attcaaccac caaacatcca
     1681 cctcatgaat aaatacgctg ccataacggc ccaggtaata agtaaacatt taagctgaaa
     1741 cagcataacc agtgcctcca acgcttctat ttattgcgat aaaataacac catcctttga
     1801 taaaaagtca cacgcccatc aataaatcca aaaataatgt cattagttgc aggataaaat
     1861 catttgagct tttcatttgt tatatttaac gcatgatgta ttaatggcaa gaatatgaga
     1921 gaaactcata cctgacgtaa aaatcactct cttatgtaat atttacttat taacactctg
     1981 tttacctaac atgcgctccg cactacacta tgtgggcaat tttttaagag gtggtaatga
     2041 acgattataa aatgacgcca ggtgagcggc gcgcgacctg gggtttaggg accgtatttt
     2101 cattgcgcat gctgggcatg tttattgtac tgccggttct gactacgtac ggtatggcac
     2161 tgcaaggcgc cagcgaagca ttaatcggta ttgccattgg tatttatggt ctgacccagg
     2221 ccgtttttca gattccgttt ggcctgcttt ccgaccgcat tggtcgaaaa ccattaattg
     2281 tcggtgggct ggcagtgttt gccgccggta gcgttatcgc tgcgctttct gactccatct
     2341 ggggaattat tctgggccgg gcgctacaag gctccggtgc gattgccgct gccgttatgg
     2401 cgctgctttc cgatctcacg cgcgaacaaa accgtaccaa agcaatggcg tttatcggcg
     2461 tgagctttgg cattaccttt gccattgcga tggtgcttgg cccgatcatc actcacaaac
     2521 ttgggctgca cgcgctgttc tggatgatcg ctattctggc aacgaccggc attgcgttga
     2581 ccatttgggt tgtgcccaac agtagcactc acgtacttaa tcgtgagtcc ggaatggtga
     2641 aaggcagttt cagtaaagtg ctggcggaac cgcggctgct gaaactcaac tttggcatta
     2701 tgtgtctgca tattttgctg atgtcgacgt ttgttgccct gcccggacaa ctggctgatg
     2761 cggggttccc ggcggctgaa cactggaagg tctatctggc gacaatgcta atcgcctttg
     2821 gctcggtcgt gcctttcatt atctacgctg aagttaagcg caaaatgaag caagtctttg
     2881 tcttctgcgt cgggttgatc gtggttgcgg aaattgtgtt gtggaacgcg caaacgcagt
     2941 tctggcaact ggtggtcggc gtgcagcttt tctttgtggc gtttaatttg atggaagccc
     3001 tgctgccctc acttatcagt aaagagtcgc cagcaggtta caaaggtacg gcgatgggtg
     3061 tttactccac cagccagttt cttggcgtgg cgattggcgg ttcgctgggc ggctggattg
     3121 acggcatgtt tgacggtcag cgggtatttc tcgctggcgc aatgctggcc gcagtgtggc
     3181 tggcagtcgc cagtaccatg aaagaaccgc cgtatgtcag cagtttgcgc attgaaatcc
     3241 cagaaaacgt aatggcagat gaaaaactgg aaaaatgctt actgacaacg cctggtgtca
     3301 aagatgtact gctggcgaag ttagaacatt gcgtctatgt gaaagtagac agtaaaatca
     3361 ccaatcgttt tgaaatagag cgagtaatca acgaagtatc ttattaaata ataagcacgg
     3421 tgctattcat gaaaaataaa atagcaccgt acttcgtaac accataatcc tttaaaccct
     3481 ccattattaa tacttcgctt aattccatat aactcgacga ttacattaga agtaaactat
     3541 actttttatg aaatatcagg gataaattat tcacatggaa aatacatcat gtacaaatca
     3601 ttatttacta ttatcattct actgtatagc gctagttcat atgcaaaaag cgaatcccct
     3661 cccgcaagca ctataacttt cattgcacaa atcgatcatg ttgagctaaa taaaacagca
     3721 ttatctcatg atatgaaact cgttcttgcc gatcgttttt atttcaaaac cagtaatcct
     3781 tgcgaaacaa attcaatttc tgctcgacca gaatcaatgg atattactga caaagcctat
     3841 gccaacaaat taaaaaaatt agaaactcct tttcttgatg atcgctattt ctttatgacc
     3901 atcaatcagt gtgacggtaa tggcactccc atgctgacag acataaaagt atgtacaaca
     3961 gaattatgtg gtgctgaata tacaaagaaa atgtctactg tatggctcga tgaaacttta
     4021 aaaccaactg ctaaacgtct ggcaatttat tttattcaat ctcctttacc ctatgacgag
     4081 gagaaaaaac aatggaaagt caccggatgg tatatctcat caactgataa tcagtcttca
     4141 actaaacaga ttgcatttga aggatataca gacacagata acttctccga ttctaaattt
     4201 atttcttcat ttaaatcctg gtttgaaagt ggaaagataa aaaacattgc taactataat
     4261 ccaaaaggag aaagagatgg aaaatacgaa agttattaca atagcaatgg taacgtctat
     4321 gaatctctaa gtttttctaa tggtcttatc aacggtgagt ttattacata ttatgaaaat
     4381 ggggccattg aaagtaaacg tcattttaat aacgacaaaa ttaaagatgg tgaatgccct
     4441 catttttatg aagatgggaa aatcaaacag caacatagct atctgaatag taaactcgat
     4501 ggtcctgcat atgaatatta tcctgatggc aaactcatgc aggaaaattt ctatcagcaa
     4561 agtgtgttga ttggaaacga taccagttac taccaatccg gaaaaataat ctctatacac
     4621 aatagaaatt cccgtggtca gtatgatgga gctaacgaac gttatagtga agagggtaag
     4681 ttacagtcta aatccgtcta taaagacgga aaacaaattt ctgttcaaac ctggtatgaa
     4741 aatgggcaga aagaagagga gaaacatttt gatgagcaag gccaattaaa tggtctggtt
     4801 aagcaatggt ataaaaatgg taatcttgca aaatcacaaa actataaaca tgacatactc
     4861 gatggtaaca gtgaagagtg gtacgaaaat ggcattcctg aaagtttgta cccttataag
     4921 aatggaaaaa ccgacggcat tgccaaatca tggaataagt atggtaaatt aacctattca
     4981 atcgaataca aaaatagcgt agagaatggg gcatatcgta actggagcaa aaacacaggt
     5041 aaactaacca aagaaaccct gtacgtcaat ggtatcagac agggagtgga aaaagagttt
     5101 aacgatcgca caggtaaact attaacctcc acgcaatatg ttaataataa aagacatggt
     5161 acggaggaaa cttatgatca aaacggaata aaatatatta catgctatca aaatgatcag
     5221 aaattatcgt ctttagacaa tccaacacaa ataaaagata atgcaacaac aggcgattcc
     5281 agtgctcaat ttgcattagg gaaatacgaa ttcatctgtg caaatataga tgaagggatt
     5341 aaatggttaa ccaaatctgc agagcaaaaa aaacactgat gcgatatact ttttagctac
     5401 agcctacaaa
//
LOCUS       DADLTF010000213         5337 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.215, whole genome shotgun sequence.
ACCESSION   DADLTF010000213 DADLTF010000000
VERSION     DADLTF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.215"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN749_003885"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="JN749_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HAY7370104.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="JN749_003886"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="JN749_003886"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HAY7370105.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="JN749_003887"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="JN749_003887"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HAY7370106.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="JN749_003888"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="JN749_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAY7370107.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1456 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgactttc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DADLTF010000214         5319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.216, whole genome shotgun sequence.
ACCESSION   DADLTF010000214 DADLTF010000000
VERSION     DADLTF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.216"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..608
                     /locus_tag="JN749_003889"
                     /pseudo
     CDS             1..608
                     /locus_tag="JN749_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            672..1598
                     /gene="glsB"
                     /locus_tag="JN749_003890"
     CDS             672..1598
                     /gene="glsB"
                     /locus_tag="JN749_003890"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HAY7370108.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1565..1957
                     /locus_tag="JN749_003891"
     CDS             1565..1957
                     /locus_tag="JN749_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HAY7370109.1"
                     /translation="MDETVRAFGLLRQSLDPLFARLSRSKFRSRFRLGMKERQYCLEK
                     GAPVIEQHAADFVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIP
                     QGVSLSEEQQRYIVAVIYHWLVVQMNQP"
     gene            2096..3514
                     /locus_tag="JN749_003892"
     CDS             2096..3514
                     /locus_tag="JN749_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HAY7370110.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3741..5192
                     /locus_tag="JN749_003893"
     CDS             3741..5192
                     /locus_tag="JN749_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAY7370111.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1228 a         1238 c         1433 g         1420 t
ORIGIN      
        1 tcccatcatt cttgcaggta acggctactt acttaaacat gcgccgaatg tgatgggctg
       61 tgctcagctc attgcccagg tgtttaaaga tgcgggaatc ccgcaaggcg tatatggctg
      121 gctgaatgcc gacaacgacg gtgtcagtca gatgattaaa gactcgcgca ttgctgctgt
      181 cacggtgacc ggaagtgttc gtgctggagc ggctattggc gcacaggctg gagcggcact
      241 gaaaaaatgc gtactactat ccgccaacgg ttctggcgaa tgttacccca gaaatgaccg
      301 cgtttcggga agaaatgttt ggccccgttg ctgcaatcac cattgcgaaa gatgcagaac
      361 atgcgctgga actggctaat gatagtgagt tcggcctttc agcgaccatt tttaccaccg
      421 acgaaacaca ggccagacag atggcggcac gtctggaatg cggtggggtg tttatcaatg
      481 gttattgtgc cagcgacgcg tgagtggcct ttggtggcgt gaaaaagagt ggctttggtc
      541 gtgagctttc ccatttcggc ttacacgaat tctgtaatat ccagacagtg tggaaagacc
      601 ggatctgacg cgacatattc agctctgata tactcgcagg tcttttcaga cctgcggtcc
      661 aggagtagaa agtggcagtc gccatggata atgcaatttt agaaaacatc ttgcggcaag
      721 tgcggccgct cattggtcag ggtaaagtcg cggattatat tccggcgctg gctacagtag
      781 acggttcccg attggggatt gctatctgta ccgttgacgg acagcttttt caggccggag
      841 acgcgcaaga acgtttttcc attcagtcta tttccaaagt gctgagtctc gttgtcgcca
      901 tgcgtcatta ctccgaagag gaaatctggc aacgcgtcgg caaagatccg tctggatcac
      961 cgttcaattc tttagtgcaa ctggaaatgg aacagggtat tccgcgtaat ccgttcatta
     1021 atgccggtgc gctggtggtc tgcgatatgt tgcaagggcg actaagcgca ccacggcaac
     1081 gtatgctgga agtcgtgcgc ggcttaagcg gtgtgtctga tatttcatac gatacggtgg
     1141 tagcgcgttc cgaatttgaa cattccgcgc gaaatgcggc tatcgcttgg ctgatgaagt
     1201 cgtttggcaa tttccatcat gacgtgacaa ccgttctgca aaactacttt cattactgcg
     1261 ctctgaaaat gagctgtgta gagctggccc ggacgtttgt ctttctggct aatcagggga
     1321 aagctattca tattgatgaa cctgtggtga cgccaatgca ggcgcggcaa attaacgcgc
     1381 tgatggcgac cagtggtatg taccagaacg cgggggagtt tgcctggcgg gtgggactac
     1441 cggcgaaatc tggcgttggt ggcggtattg tggcgattgt tccgcatgaa atggccatcg
     1501 cagtctggag tccggaactg gatgatgcag gtaactcgct tgcgggtatc gccgtccttg
     1561 aacaattgac gaaacagtta gggcgttcgg tttattaagg cagtctctcg atccactctt
     1621 cgcgcgttta tcccgttcaa aatttcgctc tcgctttcgt ctgggcatga aagagcgtca
     1681 gtattgcctg gagaaaggcg cgccagtcat cgaacaacat gcggcggatt ttgtcgctaa
     1741 acgtcttgct ccggcattac cggctaatga cggtaagcaa ccccccatgc gcgggcatcc
     1801 ggtgtttatc gctcagcacg ccaccgcgac ctgctgtcgc ggttgtctgg ctaagtggca
     1861 taacattccg cagggtgttt cgttaagcga agaacaacaa cgttatatcg tggcggtaat
     1921 ctatcactgg ctggttgtac agatgaatca gccatgagaa ttgcggaaaa cgataaaata
     1981 gcattcccag ggtaccttta tttgccgccg ctgttagcga acatgctatc ctttatgaga
     2041 atttacttat cacgttgaga taattgccac ccgcaattgt atggacagat aattaatgca
     2101 tgtacacccc atttcaactt tccgtttgtt ccaggaaggt catctgctac gtaatagcat
     2161 cgctattttt gcgctaacca cgctgttcta ttttattggt gcagagttac ggctggttca
     2221 cgaactttct cttttctggc cgctgaatgg cgtaatggcg ggggtgtttg cccgctatgt
     2281 ctggcttaat cgactgcatt actatgcgat cagttatgtg gcgatgctgg tttatgatgc
     2341 cataaccacc gaatggggac tggtttcact ggttatcaat ttctccaata tgatgtttat
     2401 tgttaccgtc gccttactgg tcgtgcggga taagcgtctg gggaaaaata agtatgagcc
     2461 agtaagtgcc ttacggctat ttaattactg tctgattgcc gcattattat gcgctattgt
     2521 cggggcgatt ggttcggtca gtattgatag tctggatttc tggcctttgc ttgcggactg
     2581 gttcagtgag caattctcaa cgggcgtgtt gatcgtgcct tgtatgctga cgttggctat
     2641 tcctggagtg ctgccgcgct ttaaagcaga acagatgatg cccgccatcg cgcttattgt
     2701 gtcggttatt gcctcggtag tcattggcgg agcggggagt ctggcgttcc cgctgcctgc
     2761 attaatctgg tgtgcagtgc gctatacgcc gcaggtaaca tgtctgttga cctttgtcac
     2821 cggtgcggtg gaaatcgtac tggtggcaaa ttcggtgatt gatatctcgg tcggttcgcc
     2881 gttctccatt ccggaaatgt tctccgcacg tctcggtatt gccacgatgg cgatatgccc
     2941 aattatggtt tcttttagcg tggcagcgat caatttgcta atgaagcaag ttgcgctgcg
     3001 agccgacttt gattttctga ctcaggttta ctcacggtcc ggtctttatg aggcgctgaa
     3061 aagtccatcg ctgaaacaga cacaacatct gacggtcatg ctgcttgata tcgactattt
     3121 caaaagcatt aacgataact atggacatga atgtggcgat aaagtgttaa gcgtgtttgc
     3181 ccggcatatt cagaagattg tcggtgataa ggggctggtg gcgcgcatgg gcggcgaaga
     3241 atttgctgtt gcagtgccgt cggtgaatcc tgtcgatggt ctgctaatgg cggaaaaaat
     3301 ccgtaaaggc gttgaactgc aaccattcac ctggcaacaa aaaacgcttt atctcacggt
     3361 aagtattggc gtcggtagtg gtcgcgcatc gtaccgaacg ctgaccgatg attttaataa
     3421 attgatggtc gaagccgata cgtgtctgta tcgctcgaag aaagatgggc gcaaccgtac
     3481 cagcactatg cgttacggtg aggaagttgt ctgacatctt tattcattcc gcgcgatttg
     3541 aatgcaatgt gttctaccac tttatgatcc aggaaaaggc gtatttcctc gattaaacca
     3601 tgatccgcgc cacacttttc tcataaaact gctcattaat catcaggagt tgatcaaaac
     3661 aggggttcgg cgtattaaag tttgctaacc tgtcgcacca attcaaacag gttgtatgac
     3721 taatcagaag ggaacccatt gtgaaaacac taaatcgtcg cgattttccc ggtgcacagt
     3781 atccagaacg tatcattcag tttggtgaag gtaacttcct gcgcgccttt gttgactggc
     3841 aaatcgatct cctgaatgag cacaccgatc tgaattctgg cgtggtcgtt gttcgtccga
     3901 ttgaaacttc attcccgccg tcactgagca cgcaggatgg tctgtacacc accattatcc
     3961 gtggcctgaa tgagaagggg gaagcggtca gcgacgcgcg tctgattcgc tcggttaatc
     4021 gtgaaatcag cgtctacagt gaatacgatg aattcctgaa actggcgcac aacccggaaa
     4081 tgcgctttgt tttctctaac accaccgaag cgggtatcag ctaccatgcg ggcgataaat
     4141 tcgatgacgc gccagcggta agctatccgg caaaactgac ccgtctgctg ttcgaacgct
     4201 ttagccattt caacggtgcg ctggataaag gttggatcat cattccgtgt gagttgattg
     4261 actacaatgg cgacgcgttg cgtgaactgg tgctgcgcta tgcgcaagag tgggcactgc
     4321 cagaagcatt tattcaatgg ctggatcagg ctaacagctt ctgttctacg ctggtggacc
     4381 gtatcgttac cggttatccg cgcgatgaag tggctaaact ggaagaagaa ctgggttatc
     4441 acgatggttt cctcgacacc gctgaacatt tttacctgtt tgtgattcag gggccgaaat
     4501 ccttagcgac tgagctgcgc ctggacaaat atccgctcaa cgtgctgatt gttgacgata
     4561 ttaaaccgta taaagaacgc aaagtggcaa tcctcaacgg tgcgcacacc gcgctggtgc
     4621 cggtggcgtt ccaggcaggg ctggataccg taggtgaagc gatgaacgat gctgaaattt
     4681 gcgcattcgt tgaaaaagcc atctacgaag aaattattcc ggtactggat ttgcctcgtg
     4741 atgaactgga atctttcgcc agtgcagtca ccgggcgttt ccgtaacccg tacattaagc
     4801 atcagctgct gtctatcgcg ctcaacggta tgaccaagtt ccgcacccgc atcctgccgc
     4861 agctgctggc agggcagaag gcaaacggca cacttccggc gcgccttact ttcgcattag
     4921 cggcattgat tgcgttctat cgtggtgagc gtaacggaga aacatatccg gtacaggacg
     4981 atgcacactg gctggaacgt tatcagcaac tctggagcca gcatcgtgac cgtgttatcg
     5041 gtacccagga actggtagcg attgtgttgg cagaaaaaga ccactgggag caggacctga
     5101 cgcaagtgcc tggtctggtc gagcaggttg ctaatgacct ggatgcgatt cttgaaaaag
     5161 ggatgcgcga agctgtacgt ccgttgtgct aataacaccg ttacttctga acccggtttc
     5221 gaccgggttt cttatttcac tatattagtt acaacatcct atatgccttt atcattaaca
     5281 ggtgtcacat gcagcgcctg ttaatgttgt aagcttgac
//
LOCUS       DADLTF010000215         5126 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.217, whole genome shotgun sequence.
ACCESSION   DADLTF010000215 DADLTF010000000
VERSION     DADLTF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.217"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..236
                     /locus_tag="JN749_003894"
     CDS             <1..236
                     /locus_tag="JN749_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370112.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="JN749_003895"
                     /pseudo
     CDS             276..816
                     /locus_tag="JN749_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /locus_tag="JN749_003896"
     CDS             883..2301
                     /locus_tag="JN749_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAY7370113.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /locus_tag="JN749_003897"
     CDS             2282..2752
                     /locus_tag="JN749_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HAY7370114.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JN749_003898"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="JN749_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HAY7370115.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="JN749_003899"
     CDS             3834..4751
                     /locus_tag="JN749_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HAY7370116.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGTIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="JN749_003900"
     CDS             4830..5012
                     /locus_tag="JN749_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HAY7370117.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1228 c         1462 g         1184 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtgag cctagcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg cggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatcgcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagcgcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gctagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg gacgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcgcc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DADLTF010000216         5044 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.218, whole genome shotgun sequence.
ACCESSION   DADLTF010000216 DADLTF010000000
VERSION     DADLTF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.218"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            9..746
                     /locus_tag="JN749_003901"
     CDS             9..746
                     /locus_tag="JN749_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HAY7370118.1"
                     /translation="MSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNV
                     TYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLG
                     SGNPRNFTCNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSF
                     YLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGL
                     SATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="JN749_003902"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="JN749_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HAY7370119.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="JN749_003903"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="JN749_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HAY7370120.1"
                     /translation="MHATTVKNKIMQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="JN749_003904"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="JN749_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HAY7370121.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1445 a         1078 c         1119 g         1402 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg taatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctatta tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcatgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttatttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atga
//
LOCUS       DADLTF010000217         5020 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.219, whole genome shotgun sequence.
ACCESSION   DADLTF010000217 DADLTF010000000
VERSION     DADLTF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.219"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="JN749_003905"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="JN749_003905"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HAY7370122.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JN749_003906"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="JN749_003906"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAY7370123.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="JN749_003907"
     CDS             2152..2610
                     /locus_tag="JN749_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAY7370124.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="JN749_003908"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="JN749_003908"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HAY7370125.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="JN749_003909"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="JN749_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HAY7370126.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="JN749_003910"
     CDS             3816..4493
                     /locus_tag="JN749_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HAY7370127.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="JN749_003911"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="JN749_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HAY7370128.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1220 a         1193 c         1322 g         1285 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac ccgaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga gtgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatt agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg attccaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tctccagggt aatgactcca acttactgat
//
LOCUS       DADLTF010000218         4984 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.220, whole genome shotgun sequence.
ACCESSION   DADLTF010000218 DADLTF010000000
VERSION     DADLTF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.220"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..181)
                     /locus_tag="JN749_003912"
     CDS             complement(<1..181)
                     /locus_tag="JN749_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HAY7370129.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
     gene            complement(245..2074)
                     /gene="recQ"
                     /locus_tag="JN749_003913"
     CDS             complement(245..2074)
                     /gene="recQ"
                     /locus_tag="JN749_003913"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAY7370130.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="JN749_003914"
     CDS             complement(2207..3076)
                     /gene="pldA"
                     /locus_tag="JN749_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HAY7370131.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3241..3708
                     /locus_tag="JN749_003915"
     CDS             3241..3708
                     /locus_tag="JN749_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HAY7370132.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            3760..4668
                     /gene="rarD"
                     /locus_tag="JN749_003916"
     CDS             3760..4668
                     /gene="rarD"
                     /locus_tag="JN749_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HAY7370133.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSKSKINIF"
     gene            4709..>4984
                     /locus_tag="JN749_003917"
     CDS             4709..>4984
                     /locus_tag="JN749_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370134.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTL"
BASE COUNT         1185 a         1286 c         1241 g         1272 t
ORIGIN      
        1 gcaggccaag caacgcaatc ccagcccaaa ccattacgcc gcaggtaatg cccagcacgc
       61 ccatcatcgc ttctttacgg gaacgactga cagcggtctg agagacaaaa aagaaatcgg
      121 gaccggggct cataagcgcc acaatgtgca ccatggcgac ggtgagaaat aacatcaaca
      181 tacatttgac tcgcggggga aacgtttacg gagtcttcat actggcactt ttttatgctg
      241 ctgactactc ttcgtcgtcg ccatcgacat gcgcacgaat cagcgccata aacggtttac
      301 caaagcgttc cagcttgcgc atcccaacgc cgttaacgct gagcatttcg ctggcggtga
      361 tcggcatctg ttcagccatc tcaatcaagg ttgcgtcgtt aaacaccacg tacggcggga
      421 cattactttc atcggcaatc gatttacgca gtttgcgtaa tttggcgaac agtttgcgat
      481 catagttgcc gccgaacgat ttctgcatcg ctttcggttt gagcgccacg atacgcggca
      541 cggcaagttg caaagaggat tcgccgcgca gcaccgggcg cgcggcctct gtcagttgta
      601 gggcagaatg ctgggcaata ttttgcgtca ccaggccgag atgaatcagc tggcggatca
      661 cgctcaccca atgttcatgg cttttatcac ggcccatacc atagactttc agtttgtcat
      721 gaccatagtc gcggatacgc tggttattag caccacgaat cacttccacc acataaccca
      781 tcccaaaccg ctgattcaca cgaccaatgg tggaaagggc aatctgagca tcggttgaac
      841 cgtcgtactg tttcggcgga tcgaggcaga tatcgcagtt cccgcacggc tcctgacgcc
      901 cttcgccaaa atagttcagc aataccagac gacggcaagt ttgcgcttcg gcaaacgcgc
      961 ccatcgcatt gagtttgtgg cgctcgatat cctgcaactg cccctgcggc ttctcttcca
     1021 gacaacggcg cagccacgcc atatcagccg gatcgtaaaa cagcatcgct tccgcaggca
     1081 ggccatcacg cccggcgcga ccggtttcct gataatagga ttcgatattg cgcggaatat
     1141 caaagtggac cacgaagcga acgtttggtt tattgatgcc catgccgaac gccaccgtcg
     1201 ccaccacaat ttgcagatca tcgcgctgga atttctcctg cacgtcggcg cggacattat
     1261 tttccagccc ggcatgatag gccgccgcgc taataccctt gctttgcagg cgcgcagcgg
     1321 tgtcttctac tttcgcccgg ctgttgcagt agataatgcc agacttaccg cgctgttcct
     1381 gcacgtagcg catcaactga tcgagcggtt tgaatttctc catcagcatg tagcgaatat
     1441 tcggacggtc aaaactgctg atttgaatca gcggatcgtt cagccccagc aggcgcacga
     1501 tatcctggcg cgtggtgtcg tcggctgtgg cggtcagcgc cataaacggc agcgtcggga
     1561 accgctggcg caactgaccg agcgcggcat attccgggcg gaaatcgtgg ccccattggg
     1621 agatacagtg cgcttcatcg acggctaata acaccggatt ccagtgcgcc agatgctcaa
     1681 gaaagttatc cagcatcagg cgttccgggg cgatatacag taagcgaatt tgcccggtgc
     1741 ggcagcctgt catcacttca agttgctgtt cgcgggtttg cgtcgagtta aggcacgccg
     1801 ccgccacgcc gttggcctgt agttgatcca cctgatcttt catcaacgaa atcagcggtg
     1861 aaacaaccac ggtaaggccg tttagcaata aggcaggaat ttgatagcaa agggattttc
     1921 cgccaccagt gggcataaca accaggcaat cgcgaccgga aagcacagtg tcgataattt
     1981 cttcctggcc ggggcgaaac tgttggtagc caaaggtttc ttgtaaaacc tgtttagctc
     2041 cggactccag attcaacact tccgcctgcg ccacattcac cccgattgcc tgaaataaaa
     2101 acaggcgcta ttttcagcgc ctgcgcgaga aactgcaatg cttttagtct ctgagagggt
     2161 aatcctggat gctggattcc agatttgcca accactcaac cgttcatcaa aacaagtcgt
     2221 ttagcataac ccctacaccg acacgggtct ggttgaagtt atagtcgatg agcgattctc
     2281 catagccgct gtaaacctga gtataaaggc gcacatgttt ggtgatcggg taacttaagc
     2341 ctaactccgc gccgccgtag ccggtgttcc agttgtactg tcctttcgca ctgagcaccg
     2401 catcaccgag gtgatagccg attttaagct ggtagtaacc catatatttg gtgatatccg
     2461 ggttatcgtc agtattaccc accacatacc acggcttcac ttctaccagc cagttaccgt
     2521 tttctgccat caggcgagta taaaggcggt tccagctgcg ggaggtcggg tctgaacgcc
     2581 cgttagagtc gtggttatac cccatctcca catcgcgcag cgtccaacct gcaaaacggt
     2641 aatcggtggc aaaaccgagg aacaattgcg gttcgtagtt ggtttcacga aacggtgaag
     2701 actcttcgct attggacagt tgccaccagg atttttgcgt ataagacgca cccaacaccg
     2761 agtttgggcc taaaatccca cgccacagcg gaaatgccag gctcaactga aactttactt
     2821 catccttacg cgcattttcc gcccagtcgt aactggcaat cgcttcttta ttcagatcgc
     2881 tggtttgggt gtagatgagg tagttggtgt cataaggata gagcgtgaac ggattgtcat
     2941 gctcctgcag catattggcg ataatactgc cacgcaccgc tggcgcgtca tgcacctctt
     3001 tcaccgttgc ctcttgtgca tatactgcca taggcaacat aaacaccggc aacaaccagc
     3061 cctgcagagt ccgcatcggt cgtgttctcc gtaacgaatt attgataagg tgaatgtttt
     3121 tctgcgggct attttacata cttaactcca ttgccgttag ttatcccttc ctaaagtaga
     3181 aaccaacaac aaagaagcat aaaatcaaca tttcattaac caatgaagtg taaggatgat
     3241 atgtctgccg tactgaccgc tgaacaagcc ctgaaattag tgggtgagat gtttgtttat
     3301 cacatgccat ttaaccgcgc attggggatg gaactggagc gttacgaaaa agagttcgca
     3361 cagctggcct ttaaaaatca gccgatgatg gtgggtaact gggcgcaaag cattttgcac
     3421 ggcggagtca ttgcgtcggc gctggatgtc gccgccggtc tggtgtgcgt gggaagtacc
     3481 ttaacccgcc acgaaaccat cagtgaagat gaactacgcc agcggctatc gcggatgggg
     3541 accattgatc ttcgcgttga ttatctgcgc ccaggcagag gcgagcgttt tactgctacc
     3601 agtagcctgt tgcgtgcagg caataaagtc gccgtcgccc gcgttgaatt acacaatgaa
     3661 gaacagcttt atattgccag tgccaccgcc acctatatgg taggttgaag acataaaagc
     3721 aggtaaactg ctgttaccaa ttaaaaatga gtattcccga tggatgcaaa acaaacgcgg
     3781 cagggcgtat tactcgctct tgccgcttat tttatttggg gtattgcgcc agcatacttc
     3841 aagctgattt actacgtgcc cgccgatgag atcctgacgc atcgcgtgat ctggtcgttt
     3901 ttctttatgg tggtgctgat gagcatttgc cgccagtggt cctatttaaa aacgctgatt
     3961 cagacgccac agaaaatttt tatgctggca gtctctgccg tgctgattgg tggcaactgg
     4021 ctactgttta tctgggcggt gaacaatcac catatgctgg aagcgagcct tggttacttt
     4081 attaacccgc tggtgaacat tgtgctgggg atgattttcc tcggcgagcg attccgccgg
     4141 atgcaatggc tggcggtgat tctggcgata tgtggcgtgt tggtccagct gtggactttt
     4201 ggttcgctac ctattatcgc gctggggctg gcatttagtt ttgccttcta cggcctggtg
     4261 cgcaagaaga ttgcggtaga agcacaaacc ggcatgttaa ttgaaaccat gtggctgctg
     4321 cccgtggcgg cgatttacct gtttgctatt gccgacagtt caaccagcca tatggggcaa
     4381 aacccgatgt cgctgaattt actgctgatc gccgccggta ttgtcactac cgtaccgctg
     4441 ttgtgtttta ctgccgctgc cacgcgcctg cgtctctcaa cgttaggctt ttttcagtac
     4501 attggcccga cgctgatgtt cctgctggct gtgacgtttt atggtgaaaa accgggtgcc
     4561 gataagatgg tgactttcac ctttatttgg gtggcgctgg caatttttgt gatggacgcg
     4621 atttatacgc agcgtagaac gtccaaatca aaaataaata tattctgaaa aaatatgccg
     4681 actcttgtat gagagtgggc atattgccat gctgacagga gactcacaca aggatgtaaa
     4741 atttatgtta cgtatgttta tcccaacatc taacggtaag atttccagac gccgatatat
     4801 attttcattt attttaataa atttcatttt tgcctttctt attatctttt ttaacgatgg
     4861 ggaagcaggt tttcttgtta tagtctctac catcgcactc cattatctcg tcatcaacat
     4921 gaattgccaa cgactccgtg atagtggttt catatatata aaaatttatg tttttggtac
     4981 atta
//
LOCUS       DADLTF010000219         4662 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.221, whole genome shotgun sequence.
ACCESSION   DADLTF010000219 DADLTF010000000
VERSION     DADLTF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.221"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            111..2997
                     /locus_tag="JN749_003918"
                     /pseudo
     CDS             111..2997
                     /locus_tag="JN749_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3107..3955)
                     /gene="alkA"
                     /locus_tag="JN749_003919"
     CDS             complement(3107..3955)
                     /gene="alkA"
                     /locus_tag="JN749_003919"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HAY7370135.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4089..>4662
                     /gene="yegD"
                     /locus_tag="JN749_003920"
     CDS             4089..>4662
                     /gene="yegD"
                     /locus_tag="JN749_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7370136.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQ"
BASE COUNT         1089 a         1217 c         1260 g         1096 t
ORIGIN      
        1 accagacccg gtgacatcac cccccggatc atggacgact gggggcagta atcatgccgg
       61 gtctggctgg ctaacacccc attataaggg gtacgaataa agtaacgggg ctggttgtcc
      121 tgctgacgcc cggagacgat cctctcaggg catttttgat atgggtactg tcagaatcca
      181 tcggcgctct ggcactggtg ccgctgggat tgttatttaa accacactat ctgctgcgcc
      241 atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa
      301 gctggctttc gatgctgtat ctgccgtggc cttttacttt cattattgtg ctgttgatgt
      361 ggagcgccgt gcgcctgcca cgaatggaag cctttttgat cttccttacc acggtgatga
      421 tggtgtcgct gatgatggcc gcggatccct ccctgcttgc tacgccgcgt acgtacctga
      481 tgagccatat gccgtggcta ccgtttttgc tgatcctgct gcccgccaac atcatgacca
      541 tggtgatgta tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa acccgctttc
      601 ggaacgcgat ggaatattcc gctatcggta tggcgttagt gggcaccgag ggacaatggc
      661 tgcaatccaa caaagcactc tgccagtttc tcggttacag tcaggaagag ctgtgcggac
      721 tcacctttca gcaactgacc tggccggagg atctcaataa agatctccaa caggttgaaa
      781 agctgataag cggtgaaata aacacctatt caatggaaaa acgctactac aaccgcaatg
      841 gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg ccacacggat ggcacgccgc
      901 tctattttat cgctcagatt gaagacatta acgagctaaa acgcaccgaa caggtgaatc
      961 agcaactgat ggagcgcatc acgctggcta acgaagcggg cgggattggc atctgggagt
     1021 gggagctgaa gccgaatatt tttagctggg ataagcggat gttcgagctg tatgaaattc
     1081 ctccgcatat caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc
     1141 agcatgccga aaaagtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat
     1201 tgccgtgaaa gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga
     1261 aggcgaagtc gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa
     1321 cgaggcattg tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc
     1381 cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagcgg cggagaagat
     1441 gagcggctgg acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac
     1501 ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc
     1561 gattgaacaa gatgtggtgt tgcactgccg gaacggcggc agttacgacg tgcattacag
     1621 tattacgccg ttaagtactc tggacggcag caatataggc tctgtactgg tgattcagga
     1681 cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact
     1741 gacgcatctc gccaatcgcg ccaattttga gaagcaactg cgtatcctgc tgcaaacggt
     1801 aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt
     1861 gaatgacagc gccgggcatg cggcgggtga cgctttactg cgcgaactgg cgtcgttgat
     1921 gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct
     1981 actgttgcca gactgtaata tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc
     2041 cgtgaatgat tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg
     2101 gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat
     2161 cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggttacga accacagcaa
     2221 gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg gatgattaaa
     2281 gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc ggaagcgcgt
     2341 aatttgtggc tgatttcact taagctctgg agttgcgaag gcgagattat tgatgaacaa
     2401 aaatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg acgggtattc
     2461 cacgaatttt tccagcaggc cgcaaaagcg gttgccagta aaggcataag catcgccctc
     2521 cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct tgagcagctg
     2581 gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga agcgatttta
     2641 gatcacgcag aaagcgtgca aaaactgcgg ctggcgggat gtcggatagt gctcagccag
     2701 gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga ttacctgcta
     2761 cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat gctgattacg
     2821 attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc agtcgtttta
     2881 cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg tgatgtgatt
     2941 gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat taactgagag
     3001 agcgtttgcc cattgcctga tgcgatgcta acgcgtctta tcaggcctac aaatcgctca
     3061 ttccgcaggc gggataaggc gctcgcaccg cttccggcga ccaacgtcat gcttcgtctg
     3121 gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag ggcttccagc
     3181 gctcggcata acggcggatt tgtgccggtg tcatccccag aaatcgctgt ttaatcagat
     3241 aatcatccgg cagaaaaaca tctttcgcct gccagccgcg caaggcgaaa taattcgccg
     3301 tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc acatcgcccg
     3361 gtattgtcat tggtaaggtg ccctccagcg ccgcatttgc cagatgaatc agcgcctctg
     3421 cccgtttcaa cggcatacct aacgctttta atgcctgcgg gtcggctgct gccagccgct
     3481 gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca taaagctgtg
     3541 ccactctggc ggtcaatttt gccgccatcg ccacgctcac cagttggcct aaaatcgccc
     3601 gcacgccctg ctcaaaagca tcaacactgc cgggtaaacg caatccgggc cgcgccgcgc
     3661 ctaacttgcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca agcaggcggc
     3721 tcattttcgc cagacactca gcggcaacgg gttctaaacc tgcacttaaa tttatgtgca
     3781 gagtatggcg ggctatatcc ggaatagcag tcaccacgcc gcgatattcg cccactgcca
     3841 gactacgggc ataataactg tccgcgaccg tttccacacc actcaccgca cgggcggcga
     3901 gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcaggata tacatcgcat
     3961 ctccttattc atccgctttc agcataaacg ttattcagac gttacgcttt gctttcatat
     4021 tccggttgtc gcgacggcaa catttcgcta aagtcacgcc ccttcttcac cggcatgggg
     4081 attatttcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg gcggtcatgc
     4141 gtgacggtaa accgcatttg ctaaaaatgg aaaacgacag cacgctgctg ccttcaatgc
     4201 tttgcgcgcc aacgcgtgaa gcggtaagcg aatggctgta ccgccatcat gacgttccgg
     4261 cagacgacga tgaaacgcag gcgttgttac gacgggcgat tcgttataac cgcgaagaag
     4321 atatcgatgt tacagcgaaa agcgtgcagt tcggtctttc ctcactggca cagtacattg
     4381 atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt gccagcggct
     4441 taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg ttgcacattc
     4501 gccagcaggc gcaggcgcag ctgccagaag caattactca ggcggtgatt ggtcgcccga
     4561 tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcagggg attctggaac
     4621 gcgcggcgaa gcgtgccggg tttaaggatg tggtattcca gt
//
LOCUS       DADLTF010000220         4650 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.222, whole genome shotgun sequence.
ACCESSION   DADLTF010000220 DADLTF010000000
VERSION     DADLTF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.222"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..213
                     /locus_tag="JN749_003921"
     CDS             <1..213
                     /locus_tag="JN749_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAY7370137.1"
                     /translation="PQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEEL
                     SNLVLLHPNCHRQLHSCEAGSLKKGL"
     gene            356..577
                     /locus_tag="JN749_003922"
                     /pseudo
     CDS             356..577
                     /locus_tag="JN749_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(598..660)
                     /locus_tag="JN749_003923"
                     /pseudo
     CDS             complement(598..660)
                     /locus_tag="JN749_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1110..2522
                     /gene="ospC1"
                     /locus_tag="JN749_003924"
     CDS             1110..2522
                     /gene="ospC1"
                     /locus_tag="JN749_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HAY7370138.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2626..2775)
                     /locus_tag="JN749_003925"
                     /pseudo
     CDS             complement(2626..2775)
                     /locus_tag="JN749_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2851..4548
                     /gene="ospD3"
                     /locus_tag="JN749_003926"
     CDS             2851..4548
                     /gene="ospD3"
                     /locus_tag="JN749_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HAY7370139.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1623 a          833 c          863 g         1331 t
ORIGIN      
        1 ccgcagccgt caggctgcct actcgagtca caaggctctt tgtgtccgat atgccggcag
       61 cgcattaccc atgaaactgg ctggaacgta caccataagg tgaagaaagt tatggggggc
      121 ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca gttacacagt
      181 tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac cgggaaaccg
      241 gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc cgacaaacat
      301 ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg aggaaatgga
      361 cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc aacaggacac
      421 tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg cttatgcttc
      481 cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt tcggtaaaag
      541 ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg tgatctacgc
      601 cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact cataagggaa
      661 gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc tctattaaaa
      721 aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga tttcattgca
      781 gaatacacat tccattaact ggaagcactc cataagagga atcagagaaa gtatcgtttt
      841 tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat tatgaaaata
      901 atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat tttgaaatat
      961 ctggcatcac ataacatcaa aagtatataa tacccttatc taattacatg cagtatattg
     1021 tatatatact caaaaattgt aattcatacc ctgctatata ttgtagggaa agatattaat
     1081 taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg aactcagcaa
     1141 atacccaatg caatatagat tctatggata acagattaca tacattgttt ccaaaagtga
     1201 catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta aaagatagtg
     1261 caaatattat taaagatttc tttaggaaaa ctatagcagc acagagttat agtagaatgt
     1321 tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca tcagacgcta
     1381 aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat atatctgaaa
     1441 taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg ctaattatta
     1501 attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa attttaaaca
     1561 ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac acatacgctg
     1621 atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa atctctaacc
     1681 atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt gcaaatgcgt
     1741 atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac tttgataatg
     1801 ctattccacc tgttttttac catgagcacc aatcattttt agataaattt tcagaggtta
     1861 ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta ccaatattca
     1921 atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt agaaaaagtg
     1981 aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg gatctggata
     2041 gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta agtacagaaa
     2101 acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca gcatctaatt
     2161 acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct ctttttcttt
     2221 cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt gagataacta
     2281 gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta cttagcgctc
     2341 ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt gatgtaaaca
     2401 caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata aaatatgaga
     2461 atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat aaatatattt
     2521 aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc gatatattgt
     2581 ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatggggt tcgccccgtt
     2641 gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga gcagcccccc
     2701 tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggg gacaaaccac
     2761 atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta agtctgcgtc
     2821 acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat cccatcaagg
     2881 aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac aatccagtct
     2941 ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt attaaatcta
     3001 aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt agcttcctac
     3061 tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt tgcttttccg
     3121 gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa tgtgcctgct
     3181 attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat tttcaatgaa
     3241 atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa tcacaatatg
     3301 gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat tcagctttat
     3361 gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa cttagctaaa
     3421 ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg ttatggtctt
     3481 atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat gtcctccata
     3541 atcagatggc ctaataattt acttcacccc aaagttattt atcacgcgat gcgtatggga
     3601 ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct ttcagacaat
     3661 acgttagaat tacttttggc agccaaaaat gacgatggtt tttcaggatt gcttttagct
     3721 ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga aacttctgga
     3781 cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg acgaatatcg
     3841 ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata cggaaggctt
     3901 ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct gaccgcttat
     3961 tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca aaatggacat
     4021 gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct caactcagag
     4081 gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact tctgttagca
     4141 ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa tgaggcaaaa
     4201 cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg tttatctgga
     4261 ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg gaaaatactt
     4321 cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga aggcccaaat
     4381 ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat aaaaacttat
     4441 atgggaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca cttggacaaa
     4501 aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat attatctcgt
     4561 ggtgatacac tgaatttgag gtaaccggct catttaaacc gtctggtctg ttacctccgg
     4621 ttccacaaaa ataatgtcca tcattttgaa
//
LOCUS       DADLTF010000221         4589 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.223, whole genome shotgun sequence.
ACCESSION   DADLTF010000221 DADLTF010000000
VERSION     DADLTF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.223"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            278..808
                     /locus_tag="JN749_003927"
     CDS             278..808
                     /locus_tag="JN749_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7370140.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELISN
                     ESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            815..1048
                     /gene="mbeD"
                     /locus_tag="JN749_003928"
     CDS             815..1048
                     /gene="mbeD"
                     /locus_tag="JN749_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HAY7370141.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1628..2746)
                     /locus_tag="JN749_003929"
     CDS             complement(1628..2746)
                     /locus_tag="JN749_003929"
                     /inference="COORDINATES: protein motif:HMM:NF013884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7370142.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSNKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2864..2953)
                     /locus_tag="JN749_003930"
     CDS             complement(2864..2953)
                     /locus_tag="JN749_003930"
                     /inference="COORDINATES: protein motif:HMM:NF015365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370143.1"
                     /translation="MRQHLTRKGQKSLSLSTSVEQHDKAISTI"
     gene            complement(3389..3550)
                     /locus_tag="JN749_003931"
     CDS             complement(3389..3550)
                     /locus_tag="JN749_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7370144.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
     gene            complement(4202..4393)
                     /locus_tag="JN749_003932"
     CDS             complement(4202..4393)
                     /locus_tag="JN749_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7370145.1"
                     /translation="MTKQEKTALNMARFIRRQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            4457..>4589
                     /locus_tag="JN749_003933"
     CDS             4457..>4589
                     /locus_tag="JN749_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7370146.1"
                     /translation="MLTIRVTDDEHVRLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     "
BASE COUNT         1299 a          945 c         1134 g         1211 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcaggacgct gacactccct gataatctgc cggaaacgaa acaggcgatt gctgagggcg
      121 ttacacgcgg tttaccatgc cggagagata aaaggccgtc aggatgtgat tcaggcgctc
      181 acagaggcag ggattgacgt ggtcagggtg acgcgaagca gtatcagcat tgcagacccg
      241 aacggcggga agaatgtcag actgaaagga gcgttttatg agcaatcttt tacagacggg
      301 tgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa tgctgaacga
      361 cgagttcagg aagctcgaag aatctgtaaa cagggaactg atctcaaacg aatctttaat
      421 caggaacgct atcaacgacc atacatcagc cctgaaagaa cttctggaga ggtatcagaa
      481 aacgaccgtc gatacgatgg acgcacactg gaaaaccgta ctgaaaatga gcgtgaaacg
      541 ctggctgtgg ctgattatcg tctcagtact gatgtttgcc acgaccggga gcctgctgtg
      601 gtgccagggc atgaagataa acgcgaacat gaatattctc cgcgagcaga aagagagtct
      661 ggagaagctg aacgcgaaga catggggcgt gcgttatcac gaggacagca acggacgttt
      721 tctggtgttg cccgagggga tgaaagccga aacgaactgg acgatggaaa acgggaaaca
      781 gaatgccgta aggctggtac aggagtaaca gagcatgacg gagctggaaa gacatttgct
      841 gaacgcatta gagcaactgc aacaggacta tatgcagcgg ctgaacgaat gggagagcgc
      901 cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga
      961 gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag
     1021 ccagttatac agcgagaaca ggagataagg gacgaacggc tgatacagga atgcgaacat
     1081 gcgttatccc tggagcgtga gcgacagccg gaaatacagg aacggacact ggatggccct
     1141 tcgctgggat ggtaatcaga gacaggctaa aatgttgtca gcgcgtttcc ggtgcatttt
     1201 ttgggataat ggttggtttg gggggcaccg ggaacgcgct tcagtcagac cttgctgaaa
     1261 acataggaac gcgttaaaac gcatacgggc gcgattctgg tgtgtctttt gttgctgtgt
     1321 tttgcctcta ttgccagatc gcgctcattc ccctgaactt ctgttttccc gctcaggtcc
     1381 ccgtcttctt cgcttccgaa agcatgaaaa aaatccgttt cagctgtctc tggctggcaa
     1441 ttcctgcgtg attcacatgg ctgcatagct atgcatgtgg ttaaaattta ccgggtgcga
     1501 tcgcggcagt ttttcgggtg gtttgttgcc atttttacct gtctgctgcc gtgatcgcgc
     1561 cgaacgcgtt tcagcggtgc gtacaatgag atgttatggt aaattcaagt cttagatttt
     1621 atgatgttta attagctttt tttgcgttta taatgttcat cttttttatt aaaattgatt
     1681 caatctttga tgtgggtatt aatattttat ttgacaaata gaaagttaaa aaaaacacca
     1741 ttaatagtat aaataaatac ccaaaaacag aagcgttatt aatttcaatt attgatgtta
     1801 atctgtatat tactacatgc tgaatcaaca tggatatgta gatgcattta gcatatgagt
     1861 tgaaaaaata gaggttaaat ttcttattaa ttattaatgc tgctttgtaa atgataatgc
     1921 ttatgcacat taaaggtatt attgataact ggtgattgac tatgtttgtt ttgaactcat
     1981 taggccctat taataatact gtcaatatac atataacaac cgatagaata actgtggtgt
     2041 ttttttgtaa tttatttttg tatttaaatg caagcatccc aaccataaac tcaggaagtc
     2101 ggcaagcaaa aaaccaaaat gggaagttta aaaatgatga tgagatattt gatatcggga
     2161 atgttgtaat tgttatcaca aataaaaaaa atatgttacg tttaaatgta aaggtattca
     2221 taacgtgaaa aactatcggt gatatcacgt acagcataat aacgcaccca ataaaccact
     2281 cgcctgtaag gaaaagaaaa tcataagaga aatgggcgtt taatatacca tcaagggcaa
     2341 aaaatactgg aattattcta gtgtactggc ctcctgttgt cattattgat acgagaggga
     2401 ctgtattaat tatctcttta tttaaataag acaataaaat taagagtaaa gagataaaaa
     2461 agtaagtgat aatataagga gtaaggatcc ttactccttt gtcaaggaaa taatcaatgg
     2521 tattctttct atctagtgaa ataaatgcca aatatccaga tataaaaaaa aacaagctca
     2581 cacctacctg gccaagaacc cctccataaa atgatggtat gaaagatagc ctggtttggc
     2641 taaatatgta gccataatga gatataataa caaaaatgca ggctgttact cgcaatgtat
     2701 caattgacgg atctctatta gacataacct ttatttcgct gttcaaatta tcgcctttag
     2761 ttgatgatgt aaaattaacg ctataaataa gctacacatt ctagagcagc gaaaggatga
     2821 ttcaagtatt tttaataata aaacgcattg ataatgttta tgttcagatg gtgctgatag
     2881 ctttgtcatg ctgttccact gatgttgata acgacagaga cttttgtcct tttcttgtta
     2941 gatgctgcct cagattcgga ttatggcgtt aaaggggttt gacgctcaac cgaacggttt
     3001 cacacgttaa gagctttttg tctgataaat caaaatataa tggacattga aaaacgccct
     3061 caaaacggtg tctttttaat cgaagattgg cacaaaacgg acaccaccgt ccattagtcg
     3121 attttttaga cggcttttct cgcctaacgt gagttttcgt tcggttgagc atcagacccc
     3181 ttttatattg tagatttcta tttttagaca gttggaacag gacaattaac aacagatcta
     3241 cttgagatca tttgcttgtc gtcgtagtct ctttcccaga aacgaaaaaa ccgccctgca
     3301 aggcggtttc tcgaaggttc tcagagcgac caactctttg aaccaaggta actggcttat
     3361 gaggagcgca gtaaccaaat ctgtcctttc agtttagcct taacaggcgc ataacttcaa
     3421 gactaactcc tctaaatccg ttaccaatgg ctaccgccac tggcgatttg tcgtgtcttt
     3481 ccgggttgga ctcaagttga tagttaccgg aacgggcgtg gtagtcggac tgaacggggg
     3541 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3601 gaatgagata aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3661 ggagagcgca cgagggagcc accaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc tctgacttga gcgtcggttt ttgtgatgct cgtcaggggg gcggagccta
     3781 tggaaaaacg ccatcggcgc ggccttcttc ctgtttctgg tcttttgctc acatgttctt
     3841 tccggcgtta tcccctgatt ctgtggataa cagcatttcg tttttgagtg agtagatacc
     3901 gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc
     3961 ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggt atggcgcact
     4021 tttcatacaa tctgcactga tgccgcatgg ttaagcccgt atacactccg ctatagctac
     4081 gtgactgggt catggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg
     4141 cttgtctgct cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatat
     4201 gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg
     4261 cgtggtcgtg aagcgattca cagatatcag cctgttcatc cgcgtccagt tcgttgagct
     4321 tctccagcag cgttaatgtc tgccttctga taaagcgggc catgttaagg gcggtttttt
     4381 cctgtttagt cactgatacc tccgtgtaag ggggatttct gttcatgagg taatgatacc
     4441 gatgaaacgc gagaggatgc tcacgatacg ggttactgat gatgaacatg tccggttact
     4501 ggaacgctgt gagggtaaac agctggcggt atggatgcgg cgggtctgcc tgggtgagcc
     4561 ggttgcccgt tccgggaaac tgccgacac
//
LOCUS       DADLTF010000222         4537 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.225, whole genome shotgun sequence.
ACCESSION   DADLTF010000222 DADLTF010000000
VERSION     DADLTF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.225"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="JN749_003934"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="JN749_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HAY7370147.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JN749_003935"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="JN749_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JN749_003936"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="JN749_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HAY7370148.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="JN749_003937"
     CDS             complement(2403..2606)
                     /locus_tag="JN749_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370149.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="JN749_003938"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="JN749_003938"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAY7370150.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="JN749_003939"
     CDS             complement(3802..4014)
                     /locus_tag="JN749_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 family protein"
                     /protein_id="HAY7370151.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..>4537
                     /locus_tag="JN749_003940"
     CDS             4274..>4537
                     /locus_tag="JN749_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HAY7370152.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIF"
BASE COUNT         1104 a         1121 c         1067 g         1245 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct cattttt
//
LOCUS       DADLTF010000223         4498 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.226, whole genome shotgun sequence.
ACCESSION   DADLTF010000223 DADLTF010000000
VERSION     DADLTF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.226"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            5..1138
                     /gene="blaEC"
                     /locus_tag="JN749_003941"
     CDS             5..1138
                     /gene="blaEC"
                     /locus_tag="JN749_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7370153.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            1227..1760
                     /locus_tag="JN749_003942"
     CDS             1227..1760
                     /locus_tag="JN749_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7370154.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..1986)
                     /locus_tag="JN749_003943"
                     /pseudo
     CDS             complement(1757..1986)
                     /locus_tag="JN749_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2162..2308)
                     /gene="ecnB"
                     /locus_tag="JN749_003944"
     CDS             complement(2162..2308)
                     /gene="ecnB"
                     /locus_tag="JN749_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7370155.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2419..2544)
                     /gene="ecnA"
                     /locus_tag="JN749_003945"
     CDS             complement(2419..2544)
                     /gene="ecnA"
                     /locus_tag="JN749_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7370156.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2596..3162)
                     /gene="efp"
                     /locus_tag="JN749_003946"
     CDS             complement(2596..3162)
                     /gene="efp"
                     /locus_tag="JN749_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7370157.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3204..4232
                     /gene="epmB"
                     /locus_tag="JN749_003947"
     CDS             3204..4232
                     /gene="epmB"
                     /locus_tag="JN749_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7370158.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1157 a         1136 c         1119 g         1086 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacttg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc aggtgccgga
      421 tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 cccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtacctcc cccagaagaa aagaattacg cctggggata
      661 tcgcgaaggt aaggcagtgc atgtttcgcc aggggcgtta gatgctgaag cttatggtgt
      721 gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttacccc cccttgatat
      781 caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc ctgacagcat
      901 cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag cgattaagcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagagagag tcgccgccgc ctgtcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgtagttctc ctacgccgcc gcgtggcgtg accgtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccagacgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tggccgcgcc gactgcgcca atacccgtcc acacggcatc aaacggctaa agccgtgggt
     1921 atatttcagg ccaacggccc ataccacttc cagcagacca gcaataacta agataatcca
     1981 ggacatatca ggctccggaa atttggggcc gtccccggtg aaaagaggcg tttgcaggtc
     2041 gtcctgcaaa gctgatggtt gaacggatat tttgccaggt ggcaggggaa tttcaactat
     2101 tacctttatg acgatcgcag aaaaaacaaa cggcacgaca ctgctgtcgt accgttattg
     2161 cttattgctg cgctttcgtt gctgcgccag aaatcgcgtt accgccatca gaaatgtctt
     2221 caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa
     2281 agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt
     2341 ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat ctgatgattt
     2401 ttcggaagag aagaaaaatt agctggcagc gcgagagatg gagttgccga gatgtttgat
     2461 gtcttcgccg aaaccgcaag cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac
     2521 aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca gcctgcaggc
     2581 cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt
     2641 gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag tagacagggt
     2701 agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatcaacgat
     2761 ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac
     2821 gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt ttgcatcagc
     2881 agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag
     2941 gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag atttgaaggt
     3001 tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc
     3061 tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat
     3121 tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg
     3181 ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag
     3241 aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt
     3301 tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc
     3361 tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac gatcctcttt
     3421 tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gtccggattc tccaccgacc
     3481 cactggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac aaccgggcgc
     3541 ttttgctggt caaaggcggc tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc
     3601 cctatgccga aaatcagggc aacaagcgta actggcagac tgcacttgag tatgttgctg
     3661 cgcatccgga actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc
     3721 acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga
     3781 ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct
     3841 ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc aatgaggtag
     3901 atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc
     3961 agagcgttct gttacgtggt gtgaacgata acgcacaaac gttggcaaac ctgagtaatg
     4021 cgttgttcga tgccggcgtg atgccgtatt acctgcatgt gctagataaa gtacagggcg
     4081 cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacat
     4141 tggtgtcggg atatctggtg ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa
     4201 cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt taaacattaa
     4261 aattgcctga tgcgctacgc ttatcaggcc tacatcgtgc attgaaattg aatggatttg
     4321 caatgaactg taggccggat aaggcgttta cgccgcatcc ggcaagagca acaaacgcaa
     4381 gtacagcaac ctgtcattaa actgagaaat ttaatctttg cacgttgcat aacctttaat
     4441 tattcatatg cattgctgtt gttacttttt atatagctaa caccaataat taaccttt
//
LOCUS       DADLTF010000224         4488 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.227, whole genome shotgun sequence.
ACCESSION   DADLTF010000224 DADLTF010000000
VERSION     DADLTF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.227"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..254
                     /locus_tag="JN749_003948"
                     /pseudo
     CDS             1..254
                     /locus_tag="JN749_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            747..1493
                     /locus_tag="JN749_003949"
     CDS             747..1493
                     /locus_tag="JN749_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7370159.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1508..1930
                     /locus_tag="JN749_003950"
     CDS             1508..1930
                     /locus_tag="JN749_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HAY7370160.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1988..2344
                     /locus_tag="JN749_003951"
     CDS             1988..2344
                     /locus_tag="JN749_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370161.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2437..2655
                     /locus_tag="JN749_003952"
     CDS             2437..2655
                     /locus_tag="JN749_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 domain-containing protein"
                     /protein_id="HAY7370162.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2657..3019
                     /locus_tag="JN749_003953"
                     /pseudo
     CDS             2657..3019
                     /locus_tag="JN749_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3019..3684
                     /locus_tag="JN749_003954"
     CDS             3019..3684
                     /locus_tag="JN749_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370163.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3684..4049
                     /locus_tag="JN749_003955"
     CDS             3684..4049
                     /locus_tag="JN749_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7370164.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT         1198 a          955 c         1273 g         1062 t
ORIGIN      
        1 atgccaacac aaaggcaact aataaagata cagataaaga tctaaaaaca gatctaaccc
       61 atcccaaacc cttcccttcc ggaagggagt ttcgggattt tgtggctgga gtgcttgagg
      121 ggagattatc tggcggtact gcagcggaat tttgtaattc tgcggtggtt gcgttgcagg
      181 ctgctggcct ggatgtctgt cgtgagtatc cggtgccaga gcgtggtgac ggttgcggag
      241 ggcggattga tatcgtcgtg actgacagga acggtgtccg gtgtgggatc gagcttgacc
      301 gaaattctcc gcgacagaaa tcactgctca aaatcggtgc tgttgaaacc gggatatgtg
      361 tcttgcggcg cagtgatatc gcaaggcaca ccgagcaggg aattctggtt atcggtgggg
      421 ctgttcgcca gaaaaaattt gacccgttgt cagttgatct gcccgactgg ttgccagata
      481 cactctggca tgagtgggtc cagttcaggc aggcattgcg aaaaccgatt cgaacggagc
      541 tgggcgctaa cggggcgata cgggaactgg aaaaattccg tcagcagggt tttacacctg
      601 agcaggtgat tcgacacagc atcgccaatg aataccaggg tctgttcgcg ccgaaaggtg
      661 ttcggcctga gacgttgctc cgacaggtta acaccgtctc gttgccggac agtgcgatcc
      721 cgccaggctt cagggggtaa cagaccatga aaaatattgc gacaggaggc gttctggagc
      781 gtatccgcag actgacccca ccacatgtaa ccgccccatt cagaacggtt gcggagtggc
      841 gcgagtggca acttgctgaa ggccagaaac gttgcgagga gatcaaccgc ctgaatcgtc
      901 agttgcgggt ggaaaaaatt ctgaaccgct ccggcatcca gccgttgcac cggaagtgtt
      961 cgtttgcgaa ttaccgggcg cagaacgacg gtcagcgaca tgcactgagt caggcgaaat
     1021 ccatagctga cgaactgatg acaggctgta cgaattttgt gttcagcggt aagcccggca
     1081 ccggaaaaaa tcatcttgca gccgcgattg gcaaccggct gatggcgaag gggcgtagcg
     1141 tgattatcgt caccgtgtcc gatgtcatga gcgtgttgca tgagagctac gacaacggca
     1201 aatccggtga aaaattttta caggagcttt gtggtgttga cctgctggtc ctggatgaaa
     1261 ttggcatgca gcgggatacg aaaaacgagc aggtggtact gaaccagatt gttgatcgcc
     1321 ggacggcatc gttacgcggt gtggggatgc tgacaaatat taaccatgca gcgatgaata
     1381 cacttctcgg cgagcgggtg atggatcgca tggtcatgaa cggcgggcgc tgggtgaatt
     1441 ttaactggga gagctggcgt ccgaatgtta gccattcgag ggttgttaag tagtttcagg
     1501 aggatttatg gcgaaacctt ttactcccga acagcgggaa gaactgaaga cgcgaattgt
     1561 ggaactcgtg catcaggacg gtcgggtcac gattcggcag ttgtcagatg aaacaggtat
     1621 cagtcgtgcg tctgtcggtc gcttatgcat ggaactggtc gcaagtggtg atgtatataa
     1681 ttctggctac ggggtatttc cctctgaaca ggctcataaa gactggcaaa acgcccgcaa
     1741 aaagctatcg agggtaaagg tgaagaaacc ggttgtggtt gatcctgacc ttatctggtc
     1801 attaccagac ggagaaatac gccgctacga caggcgcctg aatataatct gtcgcgagtg
     1861 ccggaagagc gaagctatgc agcgcgtgct ggcgttttat caggggaaat ttcaggaggc
     1921 ggtactgtga gtaaaattag ctatcaggct tcaattaccg ctggtattcg catcaaagga
     1981 gaggagcatg gaaataaaac cagaagatga gttaagcaat atcgttttat ttccggtaaa
     2041 agaggatgac ccacgtaatc aggttaattt tctttatgaa ccatcggaaa gaccatattg
     2101 ccatcacgcc tctgtccggg ttgacgaaaa agagcgtcag gtccgctgta aaatctgcgg
     2161 tgcggttgtg gagccgtttg actggatgct ctctgtggca aaaagagaaa ccagactggc
     2221 agatgatgta aggctattgc gccaggagga acaggaaagg cggagaaata tagaaaagct
     2281 gatacagatt gagcgtaacg cgaaagcgcg gatacgcagg gcgacaaaat ccagaactga
     2341 ataattaaat ttagcactgt taaaaattta atccttaacc ggagggattt ctgcaccctc
     2401 aaatcatcag gagaccgccc gaaagggcgg taatgaatgg ttacattatt tagaaaaaaa
     2461 tatccgcgaa agagtagaac aacagaattc ctgtttctca ttctgtttat cgtgttgatg
     2521 acaccgatat ccccgctaat ttttgtctgg ataatcggaa aaataattga gccagttatt
     2581 gaattgtata acgacgtggt atgggcgtca ttcaacacac tgcacaataa aattaatccg
     2641 tataaggaaa gctgatatgg cactgacgaa aaaacaacgt gcagaactgc gcatgaagtt
     2701 cggtggtcgc tgcgcttatt gcggctgcga acttggcgaa aagtggcatg cagaccatgt
     2761 aaaaccggtc attcgttttg atggaaatat gcttcaccag gaacgtgacg atatatccaa
     2821 catggtacca gaatgccacc catgcaatct gcacaagcat tgcagtagcc tagaagatta
     2881 tcgacgaatt atcagtgatg gtcgtcgtga attccttgcg tccgggaaag gcaaagcgct
     2941 ggttcgtatg ggattggttg aaatgaaatc tgacccggtt gtgttctggt ttgaaaaata
     3001 tcaagaaggg gctacggcat ggccactatt accaaagagc gactgcaatg gctggctaac
     3061 atttctggcc gcgatgatat tgacgatata gacggcggtg aaattcgtga actggcgctt
     3121 atcgctctgg catcactgga agcagagcct gtagcggaat gcattgttga agatgggggc
     3181 atgtgtattg acgggttcgg tgagtatgtg ggtcactcgc tgcctgatgg aatgcatgag
     3241 ctttatgctg ccccgcgaat gcgacaacct gatggatgga aagccgtagc tgtagcgtgg
     3301 aaggtgacgt ttactcaagt tgaccaggaa tctaatacgt tcactgctat atattttgac
     3361 aaagcggaag ttgagcgttg ggtacgactg cataaagcat gtgattttcg ggcagatata
     3421 acaccgcttt acgcagcgcc ggcagtgccg gttgcagtaa acgacgacat ggcttacgca
     3481 ttccatcacg cactgtcaga ttcatcgctt ggctctggtg aaatcgaaga aattaaaacc
     3541 ggtttgcgtg ctgcctttgc caacgtcact atccaaccag agccgatagt gccggatgaa
     3601 atcgggccaa acgatagtaa tacgtttgat tatgttgatg gctggaacgc ctgccgcgct
     3661 gccatgctta agggagataa ataatgatta atcgaaccaa actggagcac attctcgaat
     3721 atgccaggca gcagaaatgt attgggcaac tttgtaaaat tccaccagga gatatggttg
     3781 aaatcgtgga aatggccatg cgtaaggctg gcaactctcc ggtaactctg gctggctgga
     3841 taagctgtag tgatgcagtt cctgctgaat attgcgatgt gattcttctc gatgatctcg
     3901 ggaatgtatt ccccggttcc tgggataagg ttttttgccc cactcgtggc gggaataaga
     3961 tggcttttgt ggacaaagac ggcgttgaag tagagagctc aactcactgg atgccgctac
     4021 cggaaccacc gcaggaggtg aatcaatgac ctggcctgag gcattcacca cggtaggaat
     4081 tgcgatggcg gtggcgctgg tggtgtattc gatttgccgt tggggttaac aaacaaaaac
     4141 cccggattga tggtccgggg tttttgaagg aaacaaaaca gaaacagcaa ttgccgttac
     4201 ctgttgttac catggcaagt aaacgtatct caggcgagcg cattgcgccg ttctgacgca
     4261 gataaattag cctggataga aggtgctggc aataaaaaat agcgttttct tatcggtatc
     4321 ggtaagattg ctgcgggtgc ttgaggctgt ctgcctcggg catgccactg taaggcagac
     4381 agagaaaagc cccagttaac attacgcgtc ctgcaagacg cttaacatta atctgaggcc
     4441 caatctatgt ctcacaaatg taggttagcc tcttacgtgc cgaaaggc
//
LOCUS       DADLTF010000225         4484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.228, whole genome shotgun sequence.
ACCESSION   DADLTF010000225 DADLTF010000000
VERSION     DADLTF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.228"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..158)
                     /locus_tag="JN749_003956"
     CDS             complement(<1..158)
                     /locus_tag="JN749_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7370165.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="JN749_003957"
     CDS             complement(308..469)
                     /locus_tag="JN749_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370166.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..669)
                     /locus_tag="JN749_003958"
     CDS             complement(466..669)
                     /locus_tag="JN749_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HAY7370167.1"
                     /translation="MVERTMAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAIT
                     PYTLMQAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="JN749_003959"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="JN749_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HAY7370168.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1661)
                     /locus_tag="JN749_003960"
     CDS             complement(1530..1661)
                     /locus_tag="JN749_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 domain-containing protein"
                     /protein_id="HAY7370169.1"
                     /translation="MIAYCFCEWHCSSRIMSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2161..2237)
                     /locus_tag="JN749_003961"
     tRNA            complement(2161..2237)
                     /locus_tag="JN749_003961"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="JN749_003962"
     tRNA            complement(2339..2414)
                     /locus_tag="JN749_003962"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2558..3379)
                     /locus_tag="JN749_003963"
     CDS             complement(2558..3379)
                     /locus_tag="JN749_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HAY7370170.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3394..3750)
                     /locus_tag="JN749_003964"
     CDS             complement(3394..3750)
                     /locus_tag="JN749_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7370171.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3763..4484)
                     /locus_tag="JN749_003965"
                     /pseudo
     CDS             complement(3763..4484)
                     /locus_tag="JN749_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
BASE COUNT         1221 a         1218 c          897 g         1148 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaacc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccactatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgc
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaac
     2461 ccgctcagcg gcgggttgta gaaactcttc taacgtcagg cataaaacgc ccatcattat
     2521 gacgaattta ccacagattc cggaaaaatc aaccttgtta cctagttacc ttttttaact
     2581 gccgctcagc ccatgcttct tcaatatcaa accgggtcac cagcgcatca taaaatttct
     2641 taactgtttt ttcccatgac gcgcgtgtta tctggtttgt cacctcgcat atagcattaa
     2701 atgcctccgt tgatggtagt ctttcatagc cacgaccacc acaacgctgg cagtctctga
     2761 taacaggcat accacgtttt accgactctt cacgatgaat ggcgacacca cgcccacggc
     2821 aatccttaca ggcggtggaa acctcaccct ttccgccaca ctccggacag gcaactttta
     2881 ccacctccct gacttttttc cattcttccc agtaagacgg atacacacct ttcgtacact
     2941 ttgcccatac cggcggctta ccatccggat actggatctt gtttgtaaaa actatgcttt
     3001 caataaattt ttccccatag caacaagggc actgcttttt actcgctgcg ctgcgggcat
     3061 aatcctcaaa agcgtacgaa gccataatgc gcatcactac cggttttatt tctgccggaa
     3121 gttttctcaa cgccgccaca cgatcgcacc gactgagtgc ataatctgcc agtaattctg
     3181 ttgcccgcgc cctgtcattc atactgatgc ccattttccc caggaacgca gaaaaaccca
     3241 tctcagcccg attctgtgtc atgccctgcg cggccatcac atcagtgata ctcagcgcat
     3301 cttttgacgt tgaggccgat gcatcggtca ggccagggga ttttggggag tagtatttcg
     3361 gtaaatcttc cagtttcatt ttttgacctg ctcttaatgc attatggggt aaatcttcac
     3421 ccccagacgt ccaccagata ctggctgacc acgaacgata ttgatttcat caaactgctc
     3481 atcgtccatt aacactcccg catgcgtcag cgcatccagc ggtgctttca ggatattgtc
     3541 caggtcgcga cgacgcttat ccggtggctc tgcaatcacc tttatcgcca gccttccgga
     3601 caggcttaat ttcagccgct gctggcgaac aataagcgcc acagcccggc gataacgctt
     3661 tccctcctcc gagataaaat atgtgctgcc acggcgtcgc cagtaagtgt tcaccgtcgg
     3721 cgggtaaggt aaaaccaaat ctatgagcat cagtcacctc ttttacccaa gcacgccagt
     3781 tgcaaaggcg tgatcaagaa aacgaaaaat taaatcaacc tgagaaccat gcttttcttc
     3841 gaacgccaga ggatccgcat gaagctcgtt gtgatgctcc cgacacagcg gtagcgtgaa
     3901 aatatcgtga gattttgtcc ccattccgcc ctgaccatga ccaatcaggt gatggggatc
     3961 gtcggctggc ttaccacaac acgcacacgg ctgtgtcttc acccagcgtt tgtatttctc
     4021 gttaacccag ctgcgacgtt taggtcgttt catgaaagat tccggagact caggatcaac
     4081 ggcaatgctg accaccgtct tttcctgttg tgggttctgt tgctggtggg cgtgaggcag
     4141 cggcgcaaga ttttttgtgc gctgtttcag tatgctggtg gcggtctgct ctcccggtac
     4201 gatgtcgctt tcacggtaca ttgagcggat tttttccgca cgcaacccca gcgaacgacg
     4261 taataccgct tccggtagcg cgtccgccac ctgattgcgg accgcccact aggataattc
     4321 agccagagat aattcacgct cctgcgtacc gcttattgcg tgaccgatga cgtcaatcat
     4381 ccatgctgac aggttttgat gagcaagttg ctcgagtgat tcggatgtct ggtcacgcag
     4441 ctggttgtcg cagtgccagc acaacaccat tgcgccggta ccat
//
LOCUS       DADLTF010000226         4439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.229, whole genome shotgun sequence.
ACCESSION   DADLTF010000226 DADLTF010000000
VERSION     DADLTF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.229"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..213)
                     /locus_tag="JN749_003966"
     CDS             complement(<1..213)
                     /locus_tag="JN749_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HAY7370172.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="JN749_003967"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="JN749_003967"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAY7370173.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="JN749_003968"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="JN749_003968"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HAY7370174.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="JN749_003969"
     CDS             2894..3634
                     /locus_tag="JN749_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7370175.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="JN749_003970"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="JN749_003970"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HAY7370176.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4439)
                     /locus_tag="JN749_003971"
     CDS             complement(4192..>4439)
                     /locus_tag="JN749_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370177.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1100 a         1106 c         1232 g         1001 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tctggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttg
//
LOCUS       DADLTF010000227         4428 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.230, whole genome shotgun sequence.
ACCESSION   DADLTF010000227 DADLTF010000000
VERSION     DADLTF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.230"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(97..1836)
                     /gene="flhA"
                     /locus_tag="JN749_003972"
     CDS             complement(97..1836)
                     /gene="flhA"
                     /locus_tag="JN749_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HAY7370178.1"
                     /translation="MLSRSDLLTLLTINFIVVTKEAERISEVSARFTLDAMPGKQMAI
                     DADLNAGLINQAQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLSGGVC
                     IGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDVRH
                     QLLASPSVLYTATGIMFVLAVVPGMPHLPFLLFSALLGFTGWRMSKRPQAAEAEEKSL
                     ETLTRTITETSEQQVSWETIPLIEPISLSLGYKLVALVDKAQGNPLTQRIRGVRQVIS
                     DGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDGVL
                     GNDPAYGIPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDITQ
                     LHNRLSSMAPRLAEDLSAALNYSQLLKVYRALLTEGVSLRDIVTIATVLVASSTVTKD
                     HILLAADVRLALRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDSVP
                     VDPNMLNQFQSTMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYNEV
                     PDELELKIMGALM"
     gene            1781..2566
                     /gene="lafU"
                     /locus_tag="JN749_003973"
     CDS             1781..2566
                     /gene="lafU"
                     /locus_tag="JN749_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HAY7370179.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            2637..3692
                     /gene="dinB"
                     /locus_tag="JN749_003974"
     CDS             2637..3692
                     /gene="dinB"
                     /locus_tag="JN749_003974"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAY7370180.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3744..4037
                     /gene="yafN"
                     /locus_tag="JN749_003975"
     CDS             3744..4037
                     /gene="yafN"
                     /locus_tag="JN749_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HAY7370181.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            4040..>4428
                     /gene="yafO"
                     /locus_tag="JN749_003976"
     CDS             4040..>4428
                     /gene="yafO"
                     /locus_tag="JN749_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HAY7370182.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFR"
BASE COUNT         1058 a         1159 c         1199 g         1012 t
ORIGIN      
        1 tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat aaggcgttta
       61 cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc ccataatctt
      121 caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg gcgcaaacaa
      181 ccttgcataa cgcgccagca aagggcgtag ctgcggcggc accagcagca ccggatcttt
      241 ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact ggttgagcat
      301 attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg cctgattcac
      361 tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca gctcctgctt
      421 gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg ccgccagcag
      481 aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg tgacgatatc
      541 gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt ggctgtaatt
      601 gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac ggttatgcaa
      661 ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa tcttgttcac
      721 atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca gagccttcgc
      781 tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt tccccagcac
      841 gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat ccgccggaat
      901 atccgcttca tcagccttaa tgccgttgat gaaaatagcg tactggctgg gcttgaggcg
      961 gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg aaatcacctg
     1021 ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca ccagcgccac
     1081 cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt cccaactgac
     1141 ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt tctcttccgc
     1201 ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca gggcgctgaa
     1261 cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca tgatcccggt
     1321 ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat gggcgatatc
     1381 gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca gcagggaagg
     1441 gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga aggcggcgtc
     1501 ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt tgatcgccag
     1561 aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt ccatcgcgcc
     1621 gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg cctggttgat
     1681 caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg cgtccagggt
     1741 aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga taaagttgat
     1801 tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc acgggcagtc
     1861 gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc atccgccaaa
     1921 acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg tgaatgagaa
     1981 aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa ccagtatcaa
     2041 caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg ttccccaggg
     2101 attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg gcagcgcgca
     2161 gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg actcgctcta
     2221 taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca atatctacaa
     2281 caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg aagaggccgg
     2341 aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc tgctggacgc
     2401 gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtactga ccaaaagtgc
     2461 gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc agccgctggt
     2521 gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc tgaatcttta
     2581 cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg tgagcaatgc
     2641 gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg cgcgacaatc
     2701 ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg ggggtgatca
     2761 gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct actgggatgg
     2821 cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc tacaaagaag
     2881 cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa ccgttgtcac
     2941 tggatgaggc ttatctcgat gttaccgata gcgtccattg ccacggttct gcgaccctca
     3001 tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca tctgcgggcg
     3061 tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc aacggtcagt
     3121 ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg gaaaaaatcc
     3181 ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg acctgcggtg
     3241 atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt ggccgcattt
     3301 tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg ttacgaaaat
     3361 ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa tgtgaagcga
     3421 ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa cctgatttac
     3481 tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc acccaggagc
     3541 acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa acctgggatg
     3601 aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg cttgacccgc
     3661 aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata ttctggtgtg
     3721 cattattatg agggtatcac tgtatgcatc ggattctcgc tgaaaaatcg gtcaatatca
     3781 ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg gttctttcta
     3841 ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta atggacatgc
     3901 ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt gctgcaagat
     3961 tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat gatgatttca
     4021 atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc tgcaacttac
     4081 agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg acggtgtttt
     4141 gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc cattaatcaa
     4201 atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc cgccacagtt
     4261 gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg gtgcgtttga
     4321 tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac tggctcgaga
     4381 taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcat
//
LOCUS       DADLTF010000228         4416 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.231, whole genome shotgun sequence.
ACCESSION   DADLTF010000228 DADLTF010000000
VERSION     DADLTF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.231"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..203)
                     /locus_tag="JN749_003977"
     CDS             complement(<1..203)
                     /locus_tag="JN749_003977"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="FAD-binding subunit; with XdhA and XdhC
                     participates in purine salvage; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HAY7370183.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
     gene            complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="JN749_003978"
     CDS             complement(214..2511)
                     /gene="xdhA"
                     /locus_tag="JN749_003978"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HAY7370184.1"
                     /translation="MEAREATATGESCMRVDSIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATHTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASTGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAFESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            2926..3681
                     /locus_tag="JN749_003979"
     CDS             2926..3681
                     /locus_tag="JN749_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAY7370185.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            3760..3833
                     /locus_tag="JN749_003980"
     tRNA            3760..3833
                     /locus_tag="JN749_003980"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3792..3794,aa:Gly,seq:ccc)
     gene            3968..>4416
                     /locus_tag="JN749_003981"
     CDS             3968..>4416
                     /locus_tag="JN749_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7370186.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKG"
BASE COUNT         1115 a         1101 c         1130 g         1070 t
ORIGIN      
        1 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
       61 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
      121 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
      181 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
      241 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
      301 caccgcgtta cgaatagcag cggcaacagg aattatgggt ggctcaccca gtgacttatg
      361 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
      421 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
      481 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
      541 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
      601 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
      661 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
      721 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
      781 ttcaaccagc acgatatggc cttttatcag ggtcaaattc atcgctgact gatgtagcat
      841 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
      901 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
      961 atcttgtgtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
     1021 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
     1081 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
     1141 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
     1201 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
     1261 cggcaaccct gcgctgcaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
     1321 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
     1381 catagactca aaggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
     1441 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
     1501 agcgacttta ttcccccccg tagaagcgat ggagtgcccg tgagatgcat aagcgccggt
     1561 gttagacaga acatccagac tataaccttt caaggttccg tcgcggttca cgcccatttg
     1621 cccgtcaata gtaaaagcgt ggcgggtatg ggttgcgagg aaacactctt cacggctaag
     1681 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
     1741 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
     1801 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
     1861 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
     1921 catgtgacaa tgttgaataa cgggagtctg atagtgcccc tgtacctggt agtcggcggc
     1981 atcgattgtt tgttggacat tgcccgtcga catcgtgctt tgtttcagta aattgccgcc
     2041 gttatggatt ggtgcagcgt cttctgtcag cgccgcttct ggcgtggtga taacgggtaa
     2101 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
     2161 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
     2221 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagctgtag cgaatggaat
     2281 atcaggcaca tcttcccaag taaaaatcgc cagtacgccc ggtaaacttc tggcttgttc
     2341 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcgtaaca
     2401 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatgga
     2461 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
     2521 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
     2581 cagaaacgac gataagcgtt aggggaggaa ggaagtgtga tgacgttaaa aaaacacaga
     2641 tatgatacga aacaggactc tgaataggga cagtgatacg tatcaatatg agtggcgata
     2701 aaatcaatgt gataaatcag gattattacg catcaggatg ggaaataggg taaaaagtca
     2761 ggaacttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
     2821 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
     2881 tggctgctat gatatggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
     2941 ctgaataaaa aatctctggg tatcgtgatg tttttatcgg ttggactgct attggcgggc
     3001 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
     3061 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agagctggcg
     3121 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
     3181 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
     3241 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
     3301 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
     3361 aaagggattg atatctcagc tccacggggt acacctattt acgccgcggg tgcaggaaag
     3421 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
     3481 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
     3541 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
     3601 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
     3661 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
     3721 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt ttaatggtag
     3781 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccatgacact
     3841 tcttaaagtt caataactta ctgaatcata agggatatgg cttaacgcct tccgatagtt
     3901 ttactcactg taaccaacac tgtgactaac tttgttgaag tagtgagcag acctaaggtc
     3961 tcagtacatg ctcaactcaa gaacctacct ttatcagcgt aatggtgtct tttacatccg
     4021 tcttaggatg aagaccactg gtcgccttac cgcttcgctt ccttctcaca accgttataa
     4081 actagcgtca gtatctttac ggaccaagga tagacgtacc gctatggctc actcccggca
     4141 catcaagtca gcacttaaag caatccatgc agacaaccct aacgcctctt atgaggagct
     4201 acgggagcac ctgaagacca ttgtcgagtg ggaactcagt gtcagccgcg atgacctgaa
     4261 cgaccctgag tcctatcagc tttacgttga ccagtacgac gatattaagt ccaaccttcg
     4321 ggaagctgta gcgactgagc acctgactgt agaccagcat cgctatatca atgacgttat
     4381 cggtgtgctt aaagcctgtc aggatagact caaagg
//
LOCUS       DADLTF010000229         4406 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.232, whole genome shotgun sequence.
ACCESSION   DADLTF010000229 DADLTF010000000
VERSION     DADLTF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.232"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(8..1102)
                     /gene="yaiW"
                     /locus_tag="JN749_003982"
     CDS             complement(8..1102)
                     /gene="yaiW"
                     /locus_tag="JN749_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HAY7370187.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(1115..2335)
                     /gene="sbmA"
                     /locus_tag="JN749_003983"
     CDS             complement(1115..2335)
                     /gene="sbmA"
                     /locus_tag="JN749_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HAY7370188.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            2687..3844
                     /gene="ampH"
                     /locus_tag="JN749_003984"
     CDS             2687..3844
                     /gene="ampH"
                     /locus_tag="JN749_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HAY7370189.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(3845..4348)
                     /locus_tag="JN749_003985"
     CDS             complement(3845..4348)
                     /locus_tag="JN749_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7370190.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         1117 a         1165 c         1055 g         1069 t
ORIGIN      
        1 tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc gcaaaccagg
       61 ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc aacatttcgc
      121 ggggtagtga tttacccgtt ttcgcttcgg caagttgata aactttcttg tacagcgccg
      181 tctcctcaaa agagaagctg tcacctttct ctaactgacg gcgaatttcg ctgtcgctca
      241 tccccagttt tcccgccagt ttgcgcgttg ccagttcggt tttcccgggt tctttactgt
      301 cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta ctgactgcgt
      361 tctgaaacgc agcattacgg ctggcgtacc agccagcgtt aaaatcagca aagcggtata
      421 tcggtgcgct atagctggcg ggataattca gtaaatggta agaaccaaac cacaacccgc
      481 cgcggcgact gaagacttcc tgacgaactg taccgtccat tttccacgga taccctttgg
      541 tatgctgttc ggcaaaagca atgctgacct gcatcggccc accggtgcgc accggattaa
      601 gcgaaccaaa cagcgtctgc cccattggta ccatgctgat cagatcgtca aaaatcgcgc
      661 tcaattgctt ttctgtacgc accgaatcta accgttcgct ataacttttg ccgtttggcg
      721 atttgatttt tagcgcggta tgaaccagaa aagcaggaat gtgcatccgt tcagcacgac
      781 ggtcaatctc ttgccaggcg atcttgctta agcccggaac ggccggatct gcctgataat
      841 ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc ggtgccagcc
      901 cctgactttc aaaggtggtg gcgagatctt tcgcccaggc atcgcggtct ttcacgctcg
      961 caggcatctt ctgccgcacg acgctcgcta catctatcgc tttttcgccc tttttcaacg
     1021 gttgcggagc ctgactacta caccccgcta aaaccagaac cgccaacaat gacagcgaag
     1081 atagatgatt gacgcgcgac attacttctc ctttttagct caaggtatgg gttacttcct
     1141 gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag atagacatca
     1201 actcaaccag tgtggtccat gagttaatca ggtactggaa agcaccgcga acctgaccaa
     1261 aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt accggcaaca attgacggaa
     1321 acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcgg gcgatgttga
     1381 aatacatata gtgaaaatag aggcgaaaat agtttttccg tacggcgcta aacagctcgc
     1441 gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc tctttacggt
     1501 aggcggcctc tactcgctgg tttttaaact ccagccccgg cagtttgatc cctaccactg
     1561 ccagcaatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga ccatacggaa
     1621 tgtgcccgat aatcggcagc tccggcacat gcgcggagag cgttaccagc accggcagga
     1681 aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc catattctcc agcgttgaag
     1741 caaaacgcat ggtgtcttcc tgcacacgct gtgcggcacc ttcgatatga cgcagttgtt
     1801 gccagttcgc catgtaatat tcgttcatcg ccgtacgcca gcggaacacg tagtgactga
     1861 caaagaagtt gttcagtaca ctgatcacca ccgcgatcag cgcaatcccc agaaagacgc
     1921 ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg cgaacttagc gcggtttgaa
     1981 tcagatcata gaacggcgca taccaggcgt tgacggcgac cccgacttcc accaaaaacc
     2041 aagtgacgaa gataatcagc gcagtaccga gtattgacca gtattgccaa cggtgcgggc
     2101 tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac aatgtagtaa gcgtaaaaaa
     2161 tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg gaagcgccgg
     2221 tgatacgtgc cacccagtca cccccaccgg cttgccagaa gataacggca atcaatgccc
     2281 aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa aaaagactta aacatatgca
     2341 ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg accgctacaa
     2401 agcgtcagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag ttcgccagcc
     2461 tgaccataag aattgtttcg acggatttca caaaccaaag attgtttaca taagcggatt
     2521 aacacgcgca ggatcacaaa tgcaggcagt ggtgagcagg aaaaagatcg tgtttacgcg
     2581 ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca tcaatgagta
     2641 gtataaatac gctcagttac cttcattcaa tctatggaca ccaccgttga aacgtagtct
     2701 gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg cgcagccgat
     2761 cactgaaccg gagtttgcct ctgatattgt cgatcgttat gccgatcata ttttttacgg
     2821 cagcggtgcc acggggatgg cgctggttgt tatcgacggt aaccagcgcg tctttcgtag
     2881 ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag ctggattcgg tcgtgcgtat
     2941 tgcttccctc accaaactaa tgaccagtga aatgctggtg aaattgctcg accaggggac
     3001 cgtaaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg tgccgaccta
     3061 caatgggacg cccattaccc tagtgaatct ggcaacccat accagcgccc tgccccgtga
     3121 acagcccggt ggcgcggcac atcgtccggt gtttgtctgg ccaacgcgcg agcaacgctg
     3181 gaaatacctt tctacggcga agctgaaagc cgcgccaggc agtcaggcgg cgtactctaa
     3241 ccttgcgttc gacctactgg cagatgcact ggcgaatgcg tccggcaagc cttataccca
     3301 gttgtttgaa gagcagatta cccatccatt ggggatgaaa gacaccacct acaccccctc
     3361 accggatcag tgccgccgtt taatggttgc tgagcgcggt gccagtccgt gcaataacac
     3421 gctggcggca attggcagcg gcggcgtcta ttccacgcct ggcgatatga tgcgctggat
     3481 gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggcagatc gcatgcagac
     3541 gttgatttat cagcgcgcgc agtttacgaa agtgattggc atggatgtcc ccggcaaagc
     3601 ggatgcgctt ggtttaggct gggtatatat ggccccgaaa gagggtcgtc cgaggattat
     3661 tcagaagaca ggcggtggtg gcggtttcat tacctatatg gcgatgatcc cgcagaagaa
     3721 tattggcgcg tttgtggtgg tcactcgttc gccgttgacg cgctttaaaa atatgagcga
     3781 tggcatcaat gatctggtga ccgagctaag cgggaataaa ccgttggtta tccccgcatc
     3841 ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta ttcatttcga
     3901 tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac gacagaacgc gatatgcgtg
     3961 tacgttgatg gatataattc atcacaccaa tacgtgatcg caattcttca ttccagtcaa
     4021 tcattgataa taatgttgcg cgggtttgtt tggctggtag atgatatccc gtacaatttc
     4081 cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc agccccataa
     4141 tataaggagc ctgggtaata ccgataagta cgttttcttc tctacgcaga gagataactc
     4201 cttctaaaat aacgaaagta tcttcgttat tgacatcact ggaaaatata acttgctttt
     4261 cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca tttatcgagc ttaccaaatt
     4321 cctgaagagg tttaactaca gataacattt gcgcgtcctt tgcagtaatg cccgtcaaat
     4381 ccttgacggg cattatttag attaaa
//
LOCUS       DADLTF010000230         4398 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.233, whole genome shotgun sequence.
ACCESSION   DADLTF010000230 DADLTF010000000
VERSION     DADLTF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.233"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..882)
                     /gene="adiC"
                     /locus_tag="JN749_003986"
     CDS             complement(<1..882)
                     /gene="adiC"
                     /locus_tag="JN749_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HAY7370191.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWT"
     gene            complement(1019..1780)
                     /locus_tag="JN749_003987"
     CDS             complement(1019..1780)
                     /locus_tag="JN749_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7370192.1"
                     /translation="MRICSDQPCIVLLTEKDVRIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(2105..4372)
                     /gene="adiA"
                     /locus_tag="JN749_003988"
     CDS             complement(2105..4372)
                     /gene="adiA"
                     /locus_tag="JN749_003988"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HAY7370193.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         1133 a         1053 c         1121 g         1091 t
ORIGIN      
        1 cgtccagccg cccagtgaac ctaagcaacc cgcagctgcg cagaaggaaa caatggcccc
       61 agcggtgtca cccaacgcca tccgtgcggc atcaccgaat ggcgaggcag aaacgcgcag
      121 tgcggcatta gggatcatcc ccataatcgc ggtggtggag agcacatagc aaacggcggc
      181 aatcaatacc ccaccaatgg tggcgatagg gacattgcgt ttcgggtttt tcaccacacc
      241 tgcggcaacg gaggcacttt ccacaccgat gaacgaccac agcgtaacgt taagggtact
      301 ttgaatcgca ccgaaggtgc ccaggccgct gacgttccat gccgccatat aggtttcacc
      361 acggaaccag aaccagccaa atacggcaat cccgacgatg gggatcagcg ccagcacagt
      421 ggcaacggcc tgcacgcggg tgatcatttt cggaccgaca atgttcagca ggacgaagat
      481 ccacagcacc acgacgcagg tgatggttaa taccaatgga tctttcagaa tcgggaagaa
      541 gtaacttaaa tatcctacgc caatgaccac catggcgata ttgccgatcc agcaggccag
      601 ccagtagagg acgttggttt gataaccgag aaacgggcca aagcagcggc gggcgtaagc
      661 gtaagaacca ccaggacttg ggtcgaggaa cgacattttg gcgtatacca tcgagagccc
      721 cagcgcaccg ataatcgtca ccaaccatcc ataaatggca atcccgccag tggaggccag
      781 gtttgcaggt aacagaaaaa cacctgaccc cataatattc cccgacacca tcagggtgac
      841 ggggattaag cccactttgt gagcatcagc atccgaagac ataattaaac tcctgcgaag
      901 gcgagcttcg tgacaataaa ttacgtcata tcatacgcct gcattcgata gttgaattat
      961 tatcgaccgg gttatcgctg atgccgttat taatatagct cttcgttaag gttaaaaatc
     1021 aggcgacagt gcgttctctg tgctgactaa cataatgcag cggcgtcata ccgtagaagt
     1081 ctttaaagac agaaataaag tacgacgtac tgttgtagcc gcaggactgt gatacctgag
     1141 agatattttt accgtccatc attaattcat ttacggcata acgcatgcgg caggtggtga
     1201 ttatttggct ataactggtg ttttcgcttt tcaacttttt ctttaacaga cttgggctaa
     1261 gacataaaca actggctacc atactaagat tccagtcttt gtgaatatcg ctttcaataa
     1321 tttgataaac gctgtcactt acacaattac gtaacatata catcattaat gaaaggaatt
     1381 ttttactgtc gagaaaacgg gatagcaccg tgaataataa tgcgcgtgtt cgttcctttt
     1441 ctgactcagt ttctgcgggc atcatgctgt gttgcgcggc aacgcgaaag acgtctggcg
     1501 tcaggcatgg cagggtgagt atgggcgtag cgctacgttg ccatactggt atttgcgaga
     1561 gatctttatt cagaaaccgg aggtagtctt tgatgatgtc gtgactaata tgagcaacca
     1621 aagtgttgtt aagagaggag acgtcgataa tattattttc acaatttaat aacgccatat
     1681 ggttagcttt aaggctaata ggttctttcc cattcaccct tatccggaca tctttttcag
     1741 tcaataaaac aatacaaggt tggtcgctgc aaatcctcat acatgtactc ctgagtgcga
     1801 ataaaagacc agttctcgct aaagcaaagc gataccggga aatggttata taattatatg
     1861 atgaatataa acatgcataa aaataacgag ttttactaat attacaaatt tttatagtaa
     1921 tttctcatgt acatattttt ttacaggata aataacaggc aaaaaaaagc gcggggtggg
     1981 gccgcgcaaa aagtatgaca tttttgtttg cagcaatttt tatgaaaacg ttgccatact
     2041 caaaaggcct aaggccaggc ggcgtaatgt tatttaaaca attacgcctt cagcggaata
     2101 gtggttacgc tttcacgcac ataacgtggt aaataccgtc aataatttca gtcccttcag
     2161 tttcgtgttc aaatccaggg aaatggtggt cccaggattg cagcgagcgt aaataactta
     2221 cttgcggact gtttttatcg ccgaagtttt caccagacag cagcatcggg attcctggcg
     2281 gatacgggat aactgagttt gccgcgatgc gtcctggcag attttcaatg gataccagtt
     2341 cgacattgtt gtcgacaatc gcgttgtacg cttcacgcgg ggtgatttcc gccaccggca
     2401 ggccggaata ggcttcgttc aaccgtgcgc cagggttgtt ttctttcagc caggcaaaca
     2461 tggtgtcacc cagatcgtga atccccatgt tcgcgtaagt gtcaggatat tgttcaacaa
     2521 gttccggcat cacctgcgcc agcggtgtgt tggcgtcata gtggcgtttg aaggagcaaa
     2581 gggtgttaac cagagtcccc catttcccac gggttacgcc catagagaac aggaacataa
     2641 tttggaagtc agtggtgcgg gtaggcacaa tgccgtggcg accaagccag gcagtgacca
     2701 gcgccgccgg aacaccggtt tcttccagtt caccatcttc acccattccc ggagcaagga
     2761 tgctgacttt aattgggtcg agcatactcc agttatccgg aatatctttg aagccgtgcc
     2821 agctttcgcc cggatgtatt acccagcagt cctgaacggt ggtcagcagt ttggttggtg
     2881 cgtcagcaaa gtcataggtt ttgccggttt gtgggtcggt gacgacttct ttgttccacg
     2941 gtttgaagaa ccagctaccg tcagcggtga actctttata tagccgcgcc atcgcctgac
     3001 ggaaatcaac cgcttcgtta atcacttcct gtgtcagtga caggccgctg ttgccgtcca
     3061 tcatcgacac cgccacgtcg ttggatgcgc agatggcata cagcggggag gtggtggcat
     3121 gcatcatgta ggcctggttg aagcgggaga agttaatcgc cccacgacct tcacgtacat
     3181 gaatataaga agcctgtgac agcgcattca gcagtttgtg gatggagtgg gtggcgaaaa
     3241 cggtaggacc gttgtgatcg ccaggttcgc cgcgcatggc atagtgatcg gcatagatcg
     3301 ggttgaaacg tgcatagccg taccaggctt cgtcaaagtg cagacgatcg gaggtttttt
     3361 ccagcagatc ctgcgcttct ttagcgttat aacacacgcc gtcataggtg cagttggtta
     3421 ccacgcagta agacggtttt tgcccggctt tgtctttggt cagcgggctt tcactgattt
     3481 tcttttgcaa ggtttcaggt tgcatttcct gcggatagat tggcccgata atgccgtagc
     3541 ggttgcggct tggcaccata tagaccggtt tcgcgcctgt cagcatcaaa ccttgttcga
     3601 tggatttatg gcagttacgg tcaacgacca cgacatcgtt atcggtcatg caagcctgca
     3661 tgatggtgcg gttagagccg gaagtaccga cgactaccga ccaggagcga tcggcaccaa
     3721 atacgcgtgc ggcatatttt tcgctttcgc caaatgcgcc agtatggtca agcaaagaac
     3781 cgagggaagt tcgttcgatg cccatgtcgg tgcggaacag attttcacca tagtagtcat
     3841 ggtagaaacg tccggcgggt gttttggtaa aaccaacgcc gccctggtgg cctggcgctg
     3901 cccaggaata ttcatggatg tcactatatt tcatcagcgc gctgaacagt ggcggcaaca
     3961 gctgctggcg gtagcgggtc atcgcggcaa cggcgcgtcc ggcgataaag tcggcggtat
     4021 cttccagaat ccaggcgaat tcatcgacaa gctccagcag atcccggtcc attgcggcga
     4081 gggctttttc ccgatcgccc aacaggaaga ccggcacgtt ttgttggcgc tcatgaagct
     4141 taccgatcaa ttgtctgacg ttttgatgtt cgtccggatg ttccatttga tagctgaaca
     4201 tcaggcagtc aatggcttcg tttgaagaga gaatggcaaa accatcatca aaggaggtgg
     4261 atttaatcac ggtaacattt tgctggctta aagcatctgc cagacgctca acggcgttac
     4321 cgacccaggt gtcttgatgg agaaactcgc tttcaacaat taatactttc atcattgctt
     4381 acccggttat gaaggaat
//
LOCUS       DADLTF010000231         4378 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.234, whole genome shotgun sequence.
ACCESSION   DADLTF010000231 DADLTF010000000
VERSION     DADLTF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.234"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(64..822)
                     /locus_tag="JN749_003989"
     CDS             complement(64..822)
                     /locus_tag="JN749_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAY7370194.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="JN749_003990"
     CDS             complement(830..1933)
                     /locus_tag="JN749_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAY7370195.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3113)
                     /locus_tag="JN749_003991"
                     /pseudo
     CDS             complement(1943..3113)
                     /locus_tag="JN749_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3181..4206)
                     /locus_tag="JN749_003992"
     CDS             complement(3181..4206)
                     /locus_tag="JN749_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7370196.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1214 a         1147 c         1023 g          994 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 gaattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagga
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gccttgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggag cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcttgg cgtggtccgg gcaaattgcg caacacggca aagtaccaga
     2701 agaagatttg caatattacg gaagatctca atataaatcg tggaaagctt tcgtagcagc
     2761 cagttatccg aaagtctcgc cagaccgata aagaagccca aaacagaagc gaacacaata
     2821 cacaatgcag aaaccagtag cgtattgagt aagccgacaa taaaaacgcg tccgtaggtg
     2881 tcgccctgct ggtaatcgat caaatgctgg acaataccga agccagcgcc gcgatccaga
     2941 aaggcaaaac ctgaagtaat gccacgatta ttgagattcg ttacagtgtt ttgaaataac
     3001 caaccaacaa tgccgacaac agcaacaacg gcaaggatct gaaataacca ggcacgaacc
     3061 gtagggttgg caaaggagag tgagccttta acggttgagc ggcgatgaga catagaaacc
     3121 tcggaaagca taacgctgaa gagagcgccg ccgtagcgac gctcgttacc tcacaccgac
     3181 ttaacgcacg ggcggcgcgt actgaatacc gccgttattc cagagattat tttgcccacg
     3241 tttaattttc agcgggcttt ctgaacctac gttacgctca aaaatttccg agtagttacc
     3301 cacctgtttg atgatgttat aggcccattt attatccaac ttcagatcct tgccgtaatc
     3361 gccttcttta cccaacagat gtgccatatc aggcgttgct ggattagccg ctttttcatc
     3421 gacgttctgg gaattgatgc ccatctcttc agcatttagc atggcgaaaa gcgtccagcg
     3481 tacaatcgag aaccattcat catcgccacg acgaactacc ggcccaagcg gttctttaga
     3541 gataacctcc ggtaagacaa tccattcagc cgggttgctt aatttgatgc gcaaggcata
     3601 cagttgcgac tgatccgaag ccagcgtatc gcagcgacca gattccagtg ccttcgctga
     3661 ttcgtcagag cgatcgaaag tcactggtgt gtacttcata ttgtttgcct tgaaatagtc
     3721 ggcgacgttg agttcggtat cagtacccgc ctgaatacag acggtagcgc catccagttc
     3781 tttcgcgctt tttagccccg ctttatcgtg cgtcaggaag ccaatgccgt cgtaataagt
     3841 gacgccggta aatgccattc ccatcccggc atcgcgagat gaagtccagg tcgtattacg
     3901 ggagagcaaa tccacttccc ctgactgtaa agcggtgaag cgttcttttg ctgtgagtgg
     3961 ggtatatttc actttcgtgt cgtcaccaaa tacagcagcg gcaacaccac gacaaacatc
     4021 aacatcaata cctgaaaact taccgtctgc atcggcataa gagaacctag gtaatccatc
     4081 actgatcccg cattgcacaa aacctttttt ctgaacggca tcaagcgtcg cgccagcatg
     4141 cgcctgattt gcaacggcaa gcagcacgct ggcggcagcc agtgtggcta tcatcatctt
     4201 tttcataatg cttcctgtga ggcgaaaatt atcgttatag gacgtttggg aaacgtcttt
     4261 tacctgtctg tggccatcct cttataagca aaagtagtgc cagcttttgc gcacgatttc
     4321 gcgtcgcaat aagctgagtg tagacaggaa ataataaaaa cagcgccccg caatggtg
//
LOCUS       DADLTF010000232         4339 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.235, whole genome shotgun sequence.
ACCESSION   DADLTF010000232 DADLTF010000000
VERSION     DADLTF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.235"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..324
                     /locus_tag="JN749_003993"
     CDS             <1..324
                     /locus_tag="JN749_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HAY7370197.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="JN749_003994"
     CDS             383..586
                     /locus_tag="JN749_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HAY7370198.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            586..1611
                     /locus_tag="JN749_003995"
     CDS             586..1611
                     /locus_tag="JN749_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7370199.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(1664..1753)
                     /locus_tag="JN749_003996"
     tRNA            complement(1664..1753)
                     /locus_tag="JN749_003996"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1717..1719),aa:Ser,seq:cga)
     gene            1847..2644
                     /gene="mtfA"
                     /locus_tag="JN749_003997"
     CDS             1847..2644
                     /gene="mtfA"
                     /locus_tag="JN749_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HAY7370200.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2745..2820
                     /locus_tag="JN749_003998"
     tRNA            2745..2820
                     /locus_tag="JN749_003998"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2778..2780,aa:Asn,seq:gtt)
     gene            2982..4244
                     /locus_tag="JN749_003999"
     CDS             2982..4244
                     /locus_tag="JN749_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7370201.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1211 a          999 c         1036 g         1093 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gatctattta cctgcaataa
      541 aactttacca ccacgcgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagaaaat attccaagta attccaagtg
     1621 tgatttttgt cactgactta atgatgtata agtgattgaa ttttggcgga gagaggggga
     1681 tttgaacccc cggtggagtt gcccccactc cggttttcga gaccggtccg ttcagccgct
     1741 ccggcatctc tccgctcaga tggttgccat gatgccagga aatttggcat tttaacagtc
     1801 cctgtccgtg caattttgtt caagtgacga gtttgcgagc aaaacgatga ttaagtggcc
     1861 ctggaaagta caagaatcag cacatcaaac tgcccttccc tggcaggaag cactatcgat
     1921 ccccctttta acgtgtctga cagaacagga acaaagcaaa ttggtcgctc ttgccgaacg
     1981 ttttttacag caaaaacggc ttgttccttt acagggcttt gagctgaatt cattaagaag
     2041 ctgccggata gcacttctat tttgcctgcc cgttctggag ttaggactgg aatggctgga
     2101 tggttttcat gaagtcttaa tttatcctgc gccatttgtg gtcgatgatg aatgggaaga
     2161 cgatatcggt ctggtgcata accaacgtat tgttcagtca ggtcagagct ggcagcaagg
     2221 gcctatcgtt ttgaactggt tggatataca agattctttt gatgcttctg gttttaacct
     2281 gattattcat gaagtcgctc ataagctgga cacccgtaac ggcgatcgcg ccagcggagt
     2341 tccctttatt tcgttgcgtg aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa
     2401 caacattcag gaagaaatcg aattagttgg tgagaatgcg gcgagcattg atgcttatgc
     2461 tgccagtgat cctgctgaat gttttgccgt actttctgaa tatttcttta gcgccccaga
     2521 actttttgct cctcgtttcc cttcattgtg gcaacgtttc tgtcaatttt atcaacaaga
     2581 tcctttgcag agactgcatc acgctaatga tacagactcg ttttcggcga cgaatgttca
     2641 ttaattaaca actttgcaga ttaattaacc aattgaaatg gcttatgaaa tttagtgttg
     2701 acagacaagg taccgctaag taatatgcgc cccgttctca cgattcctct gtagttcagt
     2761 cggtagaacg gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca
     2821 attttctgtt ttcatgcatc cttgcgaatc tttatctgat gttgattcaa caggttagtg
     2881 tgaaaactct tctcggtgca ttttgatttt accctacgca tcgggaaaaa ttggtggtca
     2941 aatctgggat caggttagtt cgataatgga gtgaccccca tatatccctt accgacgcaa
     3001 aaatccgcac cctcaagcct tctgataaac cctttaaagt ctccgattct cacggtctgt
     3061 atctgctggt caagccgggt ggctcccgcc actggtatct caaataccgt attagcggta
     3121 aagaatcccg cattgcgctg ggtgcctatc cagccatctc cctgtctgat gcgcgacagc
     3181 aacgtgaagg tatccgtaaa atgctggcgc tgaatatcaa cccggtacag cagcgggctg
     3241 ctgaacgtgg ctcacgaaca ccggagaaag tttttaaaaa cgtggcgctg gcgtggcata
     3301 aaagtaacag gaaatggtcg cagaacaccg ccgaccgtct gcttgccagc ctgaacaatc
     3361 acatctttcc ggtcatcggg aacctacctg tatcagaact taaaccccgt catttcattg
     3421 acctgctgaa agggatcgag gaaaaaggtc tgctggaggt tgcgtcccgc acacggcagc
     3481 acctgagtaa cataatgcgc catgcggtcc atctggagtt aatcgatacg aaccctgcag
     3541 caaaccttgg cggcgtgacc acacctcctg tcagacggca ctatcctgcc ctgccgctgg
     3601 agcggctgcc tgaactgctt gaacgtattg gggcatatca tcagggccgt gaactgaccc
     3661 ggcatgccgt tctgctgatg ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc
     3721 gctggtcaga gattgatttc acaaaccgag tctggacgat acccgcgacg cgagaaccca
     3781 ttattggcgt gcgttattcc ggccgcgggg caaaaatgcg aatgccgcat atcgtccccc
     3841 tctcagaaca gtccatcgcc attctgaaac agattaagga tatcaccggt aataatgaac
     3901 tgatcttccc cggcgaccat aacccgtata agccaatgtg tgaaaacacg gtcaataagg
     3961 cactgcgggt gatgggttac gacacgaaaa aggatatctg cggtcacggc ttccgggcaa
     4021 tggcatgcag tgcgctgatg gaatcgggtt tatgggcaaa ggacgcagta gaacgccaga
     4081 tgagtcatca ggagcgcaat accgtgcgca tggcttatat tcataaggca gagcacctgg
     4141 aagcccgcaa agcgatgatg cagtggtggt cggattatct ggaagcatgc cgagaatctt
     4201 atgcaccgcc ttatacaatt ggtaaaaata agtttatccc atagtatact aacgaatccc
     4261 acactctggc aaattaccac cacctctaca ggaggtggtt ctatatagac ttttatcaac
     4321 tttatgacga aaaacacct
//
LOCUS       DADLTF010000233         4327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.236, whole genome shotgun sequence.
ACCESSION   DADLTF010000233 DADLTF010000000
VERSION     DADLTF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.236"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..758
                     /gene="yegD"
                     /locus_tag="JN749_004000"
     CDS             <1..758
                     /gene="yegD"
                     /locus_tag="JN749_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7370202.1"
                     /translation="DYEATLQEEKRVLVVDIGGGTTDCSLLLMGPQWRSRLDREASLL
                     GHSGCRIGGNDLDIALAFKNLMPLLGMGGETEKGIALPILPWWNAVAINDVPAQSDFY
                     SSANGRLLNDLVRDAREPEKVALLQKVWRQRLSYRLVRSAEECKIALSSVAETRASLP
                     FISNELATLISQRGLESALSQPLARILEQVQLALDNAQEKPDVIYLTGGSARSPLIKK
                     ALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR"
     gene            complement(770..991)
                     /locus_tag="JN749_004001"
     CDS             complement(770..991)
                     /locus_tag="JN749_004001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7370203.1"
                     /translation="MFRPNEAVTPADRQAVMAKMAAKRHRLESTLTVGATELQRFRLH
                     APARTMPLMEPLRQAAEKLAQAQADLSRC"
     gene            complement(1187..2710)
                     /locus_tag="JN749_004002"
     CDS             complement(1187..2710)
                     /locus_tag="JN749_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HAY7370204.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHGNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCLWCTLDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKLALAALHDTARERQK"
     gene            complement(2707..3468)
                     /locus_tag="JN749_004003"
     CDS             complement(2707..3468)
                     /locus_tag="JN749_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HAY7370205.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(3465..4124)
                     /locus_tag="JN749_004004"
     CDS             complement(3465..4124)
                     /locus_tag="JN749_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAY7370206.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         1106 a         1168 c         1086 g          967 t
ORIGIN      
        1 tggattacga agccaccttg caggaagaaa aacgggtgct ggtggtggat atcggcggtg
       61 gtacgactga ctgttcattg ctgctgatgg ggccgcagtg gcgttcgcgt ctcgatcgtg
      121 aagccagtct gctgggtcac agtggttgtc gtattggcgg taacgatctg gatatcgccc
      181 tagcgtttaa aaacctgatg ccactgctgg gtatgggtgg cgaaaccgaa aaaggcatcg
      241 ccctgccgat cctgccgtgg tggaatgcgg ttgccatcaa cgacgttcct gcacagagtg
      301 atttctacag tagtgccaac ggtcgtctgc ttaacgatct ggtacgcgat gcccgcgaac
      361 cggaaaaagt ggccctgtta cagaaagtct ggcgtcagcg tttaagctat cgcctggtgc
      421 gtagcgcaga agagtgcaaa attgctcttt caagcgtagc ggaaacccgc gcctcactgc
      481 cgtttatcag caatgaactg gctacgctga ttagccagcg agggctggaa agcgccctca
      541 gccagccact ggcgcggatt ctggaacagg tgcaactggc cctggataac gcccaggaaa
      601 aaccggacgt tatctacctg accggcggta gcgcccgttc tccgctgatt aaaaaagcgc
      661 tggcagaaca gttgccgggc attccgattg caggcggcga tgactttggc tccgtcaccg
      721 ccgggctggc acgctgggcg gaagtggtat ttcgttaaaa gaaaatggct cagcagcggc
      781 ttaaatcagc ctgcgcctga gccagttttt ctgccgcctg acgtaacggt tccatcaacg
      841 gcatggtccg tgctggagca tgaaggcgga atcgctgcaa ctctgtcgcg ccgacagtca
      901 acgtcgattc cagccgatgt cgtttggcgg ccattttcgc cattaccgcc tgtctgtctg
      961 ctggcgttac cgcttcgttc ggcctgaaca caaaacggcg ttcacagctc gctttccagt
     1021 cgatgaccgc ctgggtcaga tgatcaccaa accctttaac ttgcttaacg ccacgacggg
     1081 taacacctgc tgctgtttca ataccaaaag accataacgc cgctttacgc gcagggccaa
     1141 cgccgggaat agaggcaaca tcaataaaaa atccctccag aaacttctac ttctgccgtt
     1201 cccttgcagt gtcatgaagt gctgccagag cgagtttctc ttcttccggt aacccgagaa
     1261 tttcgtcctt cattttttcg agcatcgtcc gtttggcgat aaaaccttcc aggccgccta
     1321 cctgctggat ctggcggact aaatggtcat agtccatttt cgcacgatta aatgcctcac
     1381 ggcgttgctg aacttctgct ttgtacgctt tgcttgtcag actgccaata atccagatag
     1441 ccgccagcac aggaaccaaa ataatataac gcggttctgc ctgaaggccg caaagcagca
     1501 gcgataatgc tgacagtgcg atctcaagca gaatgatgta ttcgcgccgt aacaggccta
     1561 aaggaagcgg cctgccagtc ggttggaaat gatcgggtaa tggcagttgc aatgctggcg
     1621 gtgctactga cgccatcacc atcgcccaga cttttgccag cacaaaatcc ccgccggtgg
     1681 taatgacctc ttcgccgaga tcaataaaat agataacgcc ttgattatcc agcgtacacc
     1741 acaggcagtc ggtcaaatga gcgggataaa catgcattgc cgaaacggga catttcttta
     1801 actgttggcg cagagaatca agcgccgcta cccacgcttt agccgtcgga cgcccggttg
     1861 ccacgccact ttccgtgaat gcctgctgaa acatggcttc aacatcactc ggtaacatcg
     1921 atagcggaat cgatcgtggc ggcggtttta aaccacgtcg ctgattatct gacgcgtagg
     1981 cataacggaa atgggtaata tccgtctcca gcgcattacc cgcatcagag ataagcggca
     2041 caccagaata aggatgccgg ccaccaaaca agacgtgaaa aatcagcaac gcaaggccaa
     2101 aattaccgtg attttctgtg cgttcaaagc cgacaaatga cgacaaggtt tgtagctctg
     2161 gcggcgtaaa atgcgacacg ccgacttcgc ataaatgcag tgtgccattg gcgttaatct
     2221 gaaaggagtc gctatcaatc aacaccactt tgctgtcgcg acctaccata aaactgttct
     2281 ggtttacgtc acccacgaca tgcccgtgct cgtgaaccgt agcaaaagat gaagcaatat
     2341 tgcgcgcaac atagagtaga aaatcccacg cacaatgagg gtaacgttga cgacgatgtg
     2401 ccgggctata gatcatatga atcggttctt taccagaaac ttttggcatc ataaaaccga
     2461 taacttttcc gcctcgtcca ccgtgaagcg ttgcctgcgg ccaggcgaca taattcaaca
     2521 attgcgcgtc agctgtcgca gccataaagg caagtttgtc ctgttttaag gcgggtggcg
     2581 gcgtgtgata aatcttggcg acgctatcga caaactcatt gatatcataa accgcgcctt
     2641 caccgccttt gcccagttca cggcctggcg taacgcactc gccagtagca gtatataaag
     2701 tgggtttcat ggtaaccaca gcgccagtgc caacgtttta tcatcatccg tacgctcgtt
     2761 taccgccggg ctggacaaaa actgctttaa caattcaggt aataaatcga gttgttcctg
     2821 cgttgctgac gccagtccat tgaaaaacgg ggtaaagaaa ggcacatgcg gagaattatc
     2881 caacatattt aacgccagtc gctggatacc atccgtaaat gctgcaactt tatgcacacg
     2941 ctcagtgctg gtgaaagttt ccagtctgga aacggcatct tcatcggtaa tgaagtgcgt
     3001 catattggca tattcaccca ccatcggcgt aagcggcaat tgcagaccgt gaccaaaatc
     3061 caccaccacg ccgccatcgc caatctgcat aatcaaggtg ccattagccg atgaaatcaa
     3121 tcccagaaac gtgcaggcaa aatcgcgaac tgctaattct ttggcttccg cctcggcaaa
     3181 aagtcgctga cgaacggtca gcacaatatc cgtggcgagg atatcattaa gccccaattc
     3241 cccaccctgc actttttgcg acatataagc catcgcttca ttgacggcga gcatcgcgcc
     3301 ttcgcccccc tgcgaaacgc tacctgcgcc atcagcaagg aacatcacca gtaatggctg
     3361 ctggtcattt agccaggcaa cctgcatctg gcaggcatcc tggcaaggca agtccgcgct
     3421 gatgtgcgac gttccaacag cggatgcata aaccagacgc cagctcacac tgacgtccag
     3481 ccttttggcg cttccagcac aacttccgtt cccggcgtgg agcgggaaac cgaacgcagc
     3541 gaactggata accagctaaa cagttcacgg aattgtaatc cttgcagagg caaaggctga
     3601 cgaacgctga tttgtgccag agtcttcata tccgccccct gaacaccgat ggaaaagaaa
     3661 gcaaaccttt tatcttcttc cccccgaaac actttgttgg ctgcagcctg ccactcatcg
     3721 gttggtgcac catcggtaat caggaaaatc cacgggcgat aatacgaaat accatttgca
     3781 cgatactcac gtttccgctc ctctaccata tccagggctt tggtaatggc cgcacccatt
     3841 ggtgtatcgc cctgggcaaa caggatgggc ggaaaaaaat tagcggcgct ggtaaagggt
     3901 tgttccacat gcaccgggcc gaacgtcaca atgccgagtt ccactctttt taaagccagc
     3961 gaatcggcaa gcagctcatc gcgaaaggta accaatccgg tgttaagttc attaataggt
     4021 ctgccactca tcgagccaga aacatccagc agcagaatgc aggggcaacg tggttccggg
     4081 ttactggcaa aatcgctggt ggcaaacgtg atttgttcgc tcataagaaa tcctttctta
     4141 gagaattatt gaggtccggt atcataccga ctgcaataat gaaataagag caaacgaaga
     4201 aactttcaga attagtcccg gcggcgcaga aaattttgct attcgcctcg caagaacgga
     4261 gagccgtcga ttgacactaa gggcggagtg acataatttc aggagtgagg gttagggaga
     4321 ggtttcc
//
LOCUS       DADLTF010000234         4326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.237, whole genome shotgun sequence.
ACCESSION   DADLTF010000234 DADLTF010000000
VERSION     DADLTF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.237"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..157)
                     /locus_tag="JN749_004005"
     CDS             complement(<1..157)
                     /locus_tag="JN749_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370207.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASK"
     gene            complement(402..932)
                     /gene="cybB"
                     /locus_tag="JN749_004006"
     CDS             complement(402..932)
                     /gene="cybB"
                     /locus_tag="JN749_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HAY7370208.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1121..2122
                     /gene="gap"
                     /locus_tag="JN749_004007"
     CDS             1121..2122
                     /gene="gap"
                     /locus_tag="JN749_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAY7370209.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVMQLIRTLEKFAKL"
     gene            complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JN749_004008"
     CDS             complement(2164..3603)
                     /gene="aldA"
                     /locus_tag="JN749_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HAY7370210.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3800..>4326)
                     /locus_tag="JN749_004009"
     CDS             complement(3800..>4326)
                     /locus_tag="JN749_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAY7370211.1"
                     /translation="TTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSITL
                     LNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVI
                     FINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKH
                     DAVLIEAMESRSLR"
BASE COUNT         1144 a         1083 c          992 g         1107 t
ORIGIN      
        1 ttttcgatgc actaatagaa ccatcattac agacaaattt gccgcctgcc gtacagtgtg
       61 agatcccccc tttcgaacca gaacagggtt tgcgcccggc atcggcataa aaggaaacaa
      121 gcgttcccat aaacaaaata agtgccagtt ttttcatata acctccatgg tttatcgaat
      181 cttatgtgcg atttgaacgt tatacgctgg tttattttga taccgttgat gagatcaaag
      241 atcgggtgaa ttgattaaaa ataagccaga tggacgtatg ggcaaggatt atggttttat
      301 ttgtcataca aataaatatg ataggcgctt ccgatgtaga cccgtattct tcgcctgtac
      361 cacgggtcgg ttttagtaca ggcgttttct ttaaatatcc ttcaggaacg tttacgcggc
      421 atcatgcgta gaagtgtgtt gtccttccag aaatagtggt gtgccagtgc agccgcagcg
      481 tgcaacccga tgacaaaata tcccagattc gccagcgtct catgccacga ctttaagcta
      541 tcaacccgtt cgaaattggc ctctgaagcg taaggcatcg tcaaaccaaa cgcaaaccac
      601 gggttgcccc ggttatacat catcaccaaa ccaatcactg gcagcgcaat aaacaggaga
      661 taaatcacca aatgtcccaa atgcgccagt cccgtcatca tcggtttcgg cttaggtata
      721 atcggcgggg ttgggtattt cagtcttaac aaaagacgaa ccaccatcag cacgagaatt
      781 gagatgccac aggaaacatg aatcatgttg atgagcggcc gatcgctacg tgggaagaaa
      841 ccacgaaact ccattgcgca atacgctgcg ataaccagca aaaagaccag ccagtgaatg
      901 ctgatttgta accttgagta tttattttcc ataacatttc ctgctttaac ataattttcc
      961 gttaacataa cgggcttttc tcaaaatttc attaaatatt gttcacccgt tttcagtacg
     1021 ttacgtttca ctgtaagaat ggagattgcc catcccacaa tcctggtcta agcctggaaa
     1081 ggatcaattt tcatccgaac gttcctgaca ggagaaaacc atgagtaaag ttggtattaa
     1141 cggttttggt cgtatcggtc gactggtgtt gcgtcgttta cttgaagtca aaagcaacat
     1201 agacgttgtc gctattaatg atcttacttc cccaaaaatt ctcgcctatc tgctgaaaca
     1261 tgattcaaac tatggtccgt tcccctggag cgttgatttt acggaagatt cacttatcgt
     1321 tgatgggaaa agtatcgcgg tttacgccga aaaagaggct aaaaatattc cgtggaaagc
     1381 gaaaggggca gaaatcattg tcgaatgtac tggcttttat acctccgccg ataaatcgca
     1441 ggcgcatctt gatgctggtg cgaagaaggt gttgatttcc gctcctgccg gtgaaatgaa
     1501 aaccatcgtt tataacgtta atgacaacac tctggatggc aacgacacca ttgtttccgt
     1561 ggcgtcatgc accactaact gccttgcgcc gatggccaaa gccttgcatg acagtttcgg
     1621 gatagaagtc ggcacgatga cgaccattca tgcctatact ggcacccagt cactggtgga
     1681 tggcccgcgt ggtaaagatt tacgtgcttc acgcgcagcg gcagaaaata tcattcccca
     1741 cactacgggg gcggcaaaag ccattggtct ggtgatcccg gaactgagcg gcaaactgaa
     1801 aggtcatgcg caacgcgtgc cggtgaaaac aggttcggtc actgagctgg tgtccattct
     1861 cggaaaaaaa gtgactgccg aagaggtgaa taacgcactt aaacaagcaa ccaccaataa
     1921 cgagtcattt ggttataccg atgaagaaat agtctcttcc gatatcattg gcagccattt
     1981 cggttcagtg tttgatgcca cgcaaacgga aattaccgcc gtgggcgatt tacaactggt
     2041 gaaaacggtc gcctggtacg ataacgaata tggcttcgtc atgcagctta ttcgcaccct
     2101 cgaaaaattc gctaaactct gacgcgcaca ggcggaggaa aaaacctccg cctctttcac
     2161 tcattaagac tgtaaataaa ccacctgggt ctgcagatat tcatgcaggc catgtttacc
     2221 atctgcgccg ccaataccgg atttacgcca tccggcgtgg aaaccttgca tagcttcgaa
     2281 gttttcacgg ttgatgtaag tttcaccaaa cttcagccct ttaatggctt tcatcgcgac
     2341 gttcagattt tgggtataga ttgatgaggt caggccgtaa tcactgtcat tagccattga
     2401 gatagcatct tccagcgtgt caaatgcgac aactggcagc accgggccaa aggtttcctc
     2461 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     2521 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     2581 cactttttgc tcaacccttt ccagcgccgc ggcgttaatc aacggcccca tcgcaatgtc
     2641 gttgcgttca gcggggttac caaattgaac cgcctgcaac gcttcaccca gccgattgac
     2701 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     2761 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     2821 cattacgata gccggtgctt taccacccaa ttccagacac actttggtga tgtttttcgc
     2881 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     2941 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     3001 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     3061 cgtaaattca ctgggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     3121 gcgggcaatg aggaagaacg gaaagttcca cggcagaatg ccggtagtca caccaagcgc
     3181 acgtttaaac agaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     3241 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     3301 agctagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     3361 ttcgcggatc ccggcggaga ttttgcacaa ccaactggcg cgttcaatag caggcaacgc
     3421 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     3481 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     3541 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     3601 catgggcgac tcctgtgatt tatatgtttg ttttcggtga atatattgtt aattaacatc
     3661 aatcatcaga gagacggcat cgggaaagga caattctgtg gagggcttca taaaaacaag
     3721 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     3781 gttcacaaac gggcaatttt taacgtaacg agcgactttc catcgcctcg atgagcaccg
     3841 catcatgttt cagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     3901 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gtgtaaacgc ggcagctcgc
     3961 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     4021 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     4081 gtgcatcggg attgtccccg gtcatacggc ggaacgttgc catcgtgcgc cgctgcatgg
     4141 tggggtcctg tacaacgata gccgtatgaa ctcgttctac ggcctgattc aatagcgtga
     4201 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     4261 gcggaatatg ccagaactga tgagcgatat ccacctggat ggtcgcttct gctctgccag
     4321 tggtgc
//
LOCUS       DADLTF010000235         4273 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.238, whole genome shotgun sequence.
ACCESSION   DADLTF010000235 DADLTF010000000
VERSION     DADLTF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.238"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            158..827
                     /locus_tag="JN749_004010"
                     /pseudo
     CDS             158..827
                     /locus_tag="JN749_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1070..1765)
                     /locus_tag="JN749_004011"
     CDS             complement(1070..1765)
                     /locus_tag="JN749_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HAY7370212.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1758..3185)
                     /locus_tag="JN749_004012"
     CDS             complement(1758..3185)
                     /locus_tag="JN749_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HAY7370213.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3196..3915)
                     /locus_tag="JN749_004013"
     CDS             complement(3196..3915)
                     /locus_tag="JN749_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HAY7370214.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1146 a         1100 c          880 g         1147 t
ORIGIN      
        1 ccagatatgg ggaccgaatc gttatataaa acactgaggt aactctcatg cttcatatcc
       61 agtattcatg atgctggttt tttgctgata tttaacccga acattcctga tacagactat
      121 taatacaaca ttatcttcga cagattttag atatattatg agtgaacaaa tcaaacagga
      181 tatcgatctg attgagattt tatttcatct gaagaaaaag attcgtgtta tcctttttat
      241 tatggctatc tgtatggcaa tggtgctgtt gtttctgtat atcaataaag acaatacaaa
      301 agtgatttac agccttaaaa taaaccagac aacgccaggt atacttgtta gctgtgatag
      361 caataataat tttgcctgtc agaccacaat gactgaagat gttattcagc gaattactac
      421 attttttcag accagcccag atatcaagaa cagagaaata aggctggaat ggtcaggaga
      481 taagagagat ttaccaactg ctgaagagga aatatctcgc gtgcaggcgt ctattatcaa
      541 atggtatgcg tcagaatacc ataatggcag gcaagttctc gatgagatac aaacgccttc
      601 agcaattaac agtgagcttt atacaaaaat gatatacctg accaggaact ggtcattgta
      661 tccgaacggt gatggctgtg taactataag ctcaccagaa ataaaaaata aataccctgc
      721 tgccatttgc ctagctctgg gatttttttc taagcattgt aatttctgta atgttttgcc
      781 ttgtcaaaaa aatggtagat gaataccaac aaaactctgg gcagtaataa acagcattct
      841 gatagagagt gcctgtataa agctgaagtg acaatgaccg ggaagggaac ccgttttcag
      901 gaatggatgc ctgtcttttt cactgtaaat atcctggcat tactaatgcc tggatattta
      961 cagaagagca gcataaccat gacggaacgg ggttaagtca ggttgtcctt aacgctattt
     1021 tcaacctcgt atgccttctt caggtttatg ttcagatctc atatctctct cagcaatcct
     1081 caataatcag atacaccgct ttcaccggac catgaacccc gacgactttg ataagctcaa
     1141 tatccgccgt tgagctggga ccactgatga tgttaataca tgacggcatt cgttcaccgg
     1201 cctgcgcttt ctgatgcaat ttttctgcga gttgcgctac acgcggcagg attgtgcttt
     1261 tacgcaggat aaaaagagaa gattccggga gcaggctcaa tgaacgcccg cgctcggcgg
     1321 cggaaaaaag aaccacgcct cccgattcgg ttaaaccata ttcagcatac acgacaccca
     1381 ctttagcctg ctctgcctgc gagatattct cggcaccttt cgccggatcc caaacaacgg
     1441 cattgcattc ctgctgcaaa cgttcactaa tccccaattc ctccagcctc gtgtcaccgc
     1501 taatcacgac cgactgatct cccagctctt tacacagacg tattgcagct tctgccgcct
     1561 tcgcctcgct ggtcagctca cagcgcgtca acataacatc gctggcaaac tgaataaacg
     1621 cgtcacagcg ctgctgttgg ttaagttggg taagccgctc gttagcatag ttgttaagcg
     1681 gcgcatcttc tgcttgcggt tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat
     1741 tcaaaaattc gctccgatta tccattcttt ttctcctgcg cctgatgttt cttaaaccaa
     1801 ctacggaaac tctctccgtc agcttcagga agatcgcgtg cttccatcca gtcgctaatc
     1861 gcgccaaatt tgagtggtgt tttaccgcca ttgataaacc agcttgccgc atgagcgccg
     1921 gccatcatcc cgactttcca caatcctgga tgactattgg cataagcgaa catttttatc
     1981 gcccgttgct ctgcttttgc ggtgatccct ttttcagcca tcacccgacg atgacgcaaa
     2041 atcagttttg acagcggaat acgcaccgga cacacgctgt cacaagcagt gcataaagag
     2101 caggcgtagg gtaaatcttt aaaatcttta tagccgccaa gtagcggaga aatcaccgca
     2161 ccaattggcc ctggataaat agagccatat ccatgaccgc caatatggcg atatgccgga
     2221 caagtattca tacaagcccc gcagcgaata cagcgcagca catcccgaaa ttcagaggcc
     2281 agcacctcag aacgcccgtt atcgacaata accagatgaa actcttcagg accatcaacg
     2341 tgccccgctt cgcgcggccc tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg
     2401 gcactgcgcg ccagcatggt gatcaataca tctacctcgg caaacgtggg ggcaatacgc
     2461 tccattccca tcactgcaat atgcgtttta ggcagcgtgg tacacattcg cgcattacct
     2521 tcattggtca ccaggcatac cgaaccggtc tctgccaccg cgaaattaca gccggtaata
     2581 cctatttcag cactgaggaa atcttcacgg attttttgcc ggatgaataa ggtcatcgct
     2641 tcaggcgttt ccggcccctc atagcccaga cgttcgtgta gcactcgacg gatctgatgg
     2701 cgatctttat gaattgccgg gaccacaaca tgagatggcg gatcttgatc cagctggaga
     2761 atatattcac ccagatcggt ttcaatcacc tgaatgccag catcctgcaa cacatgattg
     2821 acaccaatct cttcggtcac catcgattta gatttcacca ccttccgggc atttttgcgt
     2881 tgggcaacct gtaaaatgta gcgggtagcg tcttctttgg tttttgcaaa atagacgtga
     2941 ccgccgtttt gcgtcacttt ttctgagagc tggtacagat aagcgtcgag attactcaaa
     3001 acatgatcac gtatctgggc ggcccgatcg cgccactcct cccagtgccc caattcatcg
     3061 accatttttt gccgatttgc cccaatacgc tgctgcgcgt ttgccaccgc tttgcgcatg
     3121 atcggatctt caatttgctg acggatgcgt gtcttaaaat ctgtattact ggttttgatc
     3181 gacatcttta tatcctcagc ggctcatcaa cacttcagca atatgcatca ctttgacttt
     3241 ctgcccttcc cgttgtaatc gcccactgat gttcagcagg caactcacgt cagcaccaat
     3301 taaatactca gggcggactt ccatcaggtg cacaaccttt tctttcacca tctcgccgga
     3361 tatttcggcc attttgaccg agaacgtgcc gccaaatccg cagcaggtat cctgttcagc
     3421 aaaggtcaac agctccagtc cacgcacatt tttcagcagc gtaagtggct cgtccttcac
     3481 tcccagctta cgggccaggc tacaagatgg gtgatacaca gctctccctt gcaaactggc
     3541 acctacatcg actaccccta atttattaac aataaaagag gtgagatcct gcatacgcgc
     3601 ggcaaccttt tcggcacgtg atgcccattc aggttcatcc gccagatacg tcgggtaact
     3661 ttttacggca taggtgcaag agccagccgg tgaaataatg ggatcgtcgt tatcctccag
     3721 tgcggcgatc agatttttca tccctggaat cgcttctttg atataaccgc tattgatcgc
     3781 aggctgaccg cagcatccct gtttctccgg gaaatttacg cgacagccga gtttttccag
     3841 tagcagcacg gagtctcgtg ccattcttga tttcagggcg tcaccaatac aggtgacaaa
     3901 gaaattgaca ttcacaacta atactccatt acttcatgcc catttatgcc cgatgtttcc
     3961 cccccaccgg gggatcaatc atcaataaat atggcgttct ttttaaattg gctagcaaca
     4021 taccacccct catttgtatg acaaatgata atctttcatc caaacttcaa atgcaataac
     4081 taattttatt tctttcggca tcgctcacat tttatgggga aatcgacgat catttttggc
     4141 ataagaaatc catttaaaaa tggctacatc ccttatacct attcagagct acttattcag
     4201 gggttattga gatatacact gatggtgtac aaaaaaatca ccgacaataa tcacgtatca
     4261 agtaatagtc gga
//
LOCUS       DADLTF010000236         4216 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.240, whole genome shotgun sequence.
ACCESSION   DADLTF010000236 DADLTF010000000
VERSION     DADLTF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.240"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..209)
                     /locus_tag="JN749_004014"
     CDS             complement(<1..209)
                     /locus_tag="JN749_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAY7370215.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="JN749_004015"
     tRNA            complement(324..399)
                     /locus_tag="JN749_004015"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="JN749_004016"
     tRNA            complement(406..480)
                     /locus_tag="JN749_004016"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="JN749_004017"
     tRNA            complement(597..681)
                     /locus_tag="JN749_004017"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="JN749_004018"
     tRNA            complement(690..765)
                     /locus_tag="JN749_004018"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="JN749_004019"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="JN749_004019"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAY7370216.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN749_004020"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="JN749_004020"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HAY7370217.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN749_004021"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="JN749_004021"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAY7370218.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1094 a         1047 c          936 g         1139 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtacgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggat
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 cctgacggat agcctt
//
LOCUS       DADLTF010000237         4169 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.241, whole genome shotgun sequence.
ACCESSION   DADLTF010000237 DADLTF010000000
VERSION     DADLTF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.241"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            259..852
                     /gene="tetC"
                     /locus_tag="JN749_004022"
     CDS             259..852
                     /gene="tetC"
                     /locus_tag="JN749_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7370219.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(965..2170)
                     /gene="tet(B)"
                     /locus_tag="JN749_004023"
     CDS             complement(965..2170)
                     /gene="tet(B)"
                     /locus_tag="JN749_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7370220.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2252..2875
                     /gene="tetR(B)"
                     /locus_tag="JN749_004024"
     CDS             2252..2875
                     /gene="tetR(B)"
                     /locus_tag="JN749_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7370221.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2853..3539)
                     /locus_tag="JN749_004025"
     CDS             complement(2853..3539)
                     /locus_tag="JN749_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7370222.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(3547..3933)
                     /locus_tag="JN749_004026"
     CDS             complement(3547..3933)
                     /locus_tag="JN749_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7370223.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            complement(3926..4141)
                     /locus_tag="JN749_004027"
     CDS             complement(3926..4141)
                     /locus_tag="JN749_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7370224.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
BASE COUNT         1371 a          878 c          746 g         1174 t
ORIGIN      
        1 ggtataaccc gctttattcg ccacatcatc aagcagtaaa gactgatcta aattatgttc
       61 aatccataat agaacatctt tgataaccgt tgtatgaaac tgcttatcat catatcttaa
      121 ttggatgtta ttagctttat tttgatagcg agaatgctgt tcaatataca taaaataacc
      181 taaatgttct taagattgtc acgaccacat catcatgata ccataaacat actgacggta
      241 tgttatttta aatctatcat ggaaaataaa aatcatcaac aagaaaattt taagagtacc
      301 tatcaatcac tggttaactc agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt
      361 tcaatagatg agatctcggg aaaagcgttg gtgaccaaag gtgcctttta tcatcacttt
      421 aaaaataaaa aacaattact cagtgcctgt tataagcagc aattaattat gattgatgcc
      481 tacatcacaa caaaaactga tttaacaaat ggttggtctg ccttagaaag tatatttgaa
      541 cattatcttg attatattat tgataataat aaaaacctta tccctatcca agaagtgatg
      601 cctatcattg gttggaatga acttgaaaaa attagccttg aatacattac tggtaaggta
      661 aacgccattg tcagcaaatt gatccaagag aaccaactta aagcttatga tgatgatgtg
      721 cttaaaaact tactcaatgg ctggtttatg catatcgcaa tacatgcgaa aaacctaaaa
      781 gagcttgccg ataaaaaagg ccaatttatt gctatttacc gcggcttttt attgagcttg
      841 aaagataaat aaaatagata ggttttattt gaagctaaat cttctttatc gtaaaaaatg
      901 ccctcttggg ttatcaagag ggtcattata tttcgcggaa taacatcatt tggtgacgaa
      961 ataactaagc acttgtctcc tgtttactcc cctgagcttg aggggttaac atgaaggtca
     1021 tcgatagcag gataataata cagtaaaacg ctaaaccaat aatccaaatc cagccatccc
     1081 aaattggtag tgaatgatta taaataacag caaacagtaa tgggccaata acaccggttg
     1141 cattggtaag gctcaccaat aatccctgta aagcaccttg ctgatgactc tttgtttgga
     1201 tagacatcac tccctgtaat gcaggtaaag cgatcccacc accagccaat aaaattaaaa
     1261 cagggaaaac taaccaacct tcagatataa acgctaaaaa ggcaaatgca ctactatctg
     1321 caataaatcc gagcagtact gccgtttttt cgccccattt agtggctatt cttcctgcca
     1381 caaaggcttg gaatactgag tgtaaaagac caagacccgc taatgaaaag ccaaccatca
     1441 tgctattcca tccaaaacga ttttcggtaa atagcaccca caccgttgcg ggaatttggc
     1501 ctatcaattg cgctgaaaaa taaataatca acaaaatggg catcgtttta aataaagtga
     1561 tgtataccga attcgattgc gtctcaaccc ctacttcggt atctgtatta tcacgtgtat
     1621 ttttggtttc acggaaccaa aacataacca caaggaaagc gacaatattt agcaacgcag
     1681 cgataaaaaa gggactatgc ggtgaaatct ctcctgcaaa accaccaata ataggccccg
     1741 ctattaaacc aagcccaaaa cttgccccta accaaccgaa ccacttcacg cgttgagaag
     1801 ctgaggtggt atcggcaatg accgatgccg cgacagcccc agtagctcct gtgatccctg
     1861 aaagcaaacg gcctaaatac agcatccaaa gcgcacttga aaaagccagc aataagtaat
     1921 ccagcgatgc gcctattaat gacaacaaca gcactgggcg ccgaccaaat cggtcagaca
     1981 tttttccaag ccaaggagca aagataacct gcattaacgc ataaagtgca agcaatacgc
     2041 caaagtggtt agcgatatct tccgaagcaa taaattcacg taataacgtt ggcaagactg
     2101 gcatgataag gccaatcccc atggcatcga gtaacgtaat taccaatgcg atctttgtcg
     2161 aactattcat ttcacttttc tctatcactg atagggagtg gtaaaataac tctatcaatg
     2221 atagagtgtc aacaaaaatt aggaattaat gatgtctaga ttagataaaa gtaaagtgat
     2281 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     2341 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     2401 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     2461 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     2521 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     2581 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     2641 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     2701 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     2761 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     2821 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt cttaaaagca
     2881 gcataacctt tttccgtgat ggtaacttca cggtaaccaa gatgtcgagt taaccaccct
     2941 ttagattcat aaagcgaaaa taatgcggct ccaacgtacc cacctaaatg gaaacggcgt
     3001 tcactccaat ctaaacacgc acaacagatt ttacgtgaat gtttggaagg aacgtcaatt
     3061 cccatttcat gaaaatattg aataccactt aatgtgatca ttgaaccatt ttcagtgatc
     3121 cattgctgtt gacaaaggga atcatagatc ttaacggcaa cttcgccagc taaatgatca
     3181 tagcaagtac gtgcttttcg taaatgcact ggcgtggaaa ctttggcatg tacgccatgg
     3241 tttaaggaga tccccatcat actttccatc aattcagcaa tatcttttcc tgctagccga
     3301 aaataacgat gcttgccttg agctactact gtgattagct ggcaatctaa taatttagat
     3361 aaatgactgc tcgccgttga agctgatata ttcgccacag aacttagctc agtggccgtc
     3421 caagctcgcc catccatcaa agcactgagt attttaactc gtgaaatgtc agacatagcc
     3481 gcccctatcg cggctattga ggactcaaag gtaacctctt ttcgtattaa attagccatc
     3541 gcaagttcac tttattgccc aagggagcgt aacagatgca gccatactat cattgtccgt
     3601 tattaatatc agttggttag catggtcact gtattgcact aaaatattaa tgttattctc
     3661 cgccaatact cgtgctattt cgccaagttc ccccggtttt tcctgtttta acttacgaat
     3721 taatggtgtc cggatcgcaa gtactaacag tccagcttgc tctagcgcta ttttagcttc
     3781 ctttccttgt tcaacaagaa aatgagcatg gcattcatca ccaaccgtaa atatccctcc
     3841 cccttccaat ccaatacctt tattacctaa tgtttttcct agtaatgcta attgtcctat
     3901 ttgattatct aaaacaacgt gaacatcaaa cattatcctt gtccttttta aatgaatatt
     3961 cgcgagtaat atgagcaata ctaattttgt aaaaatcaaa tattgactct cgcccttctt
     4021 gttgagcttt cgcatgtaaa acatgatttt tccattgcag aactgcgtat tcgttttccc
     4081 accaagatag cgataacatt tttccttctg tagctagact ttgaaaacgt tcaattgaaa
     4141 taaaaccagc tacatgactt aatagtggt
//
LOCUS       DADLTF010000238         4154 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.242, whole genome shotgun sequence.
ACCESSION   DADLTF010000238 DADLTF010000000
VERSION     DADLTF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.242"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(456..828)
                     /locus_tag="JN749_004028"
                     /pseudo
     CDS             complement(456..828)
                     /locus_tag="JN749_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            831..1028
                     /locus_tag="JN749_004029"
                     /pseudo
     CDS             831..1028
                     /locus_tag="JN749_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1072..1191
                     /locus_tag="JN749_004030"
                     /pseudo
     CDS             1072..1191
                     /locus_tag="JN749_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1441..2307
                     /gene="ospB"
                     /locus_tag="JN749_004031"
     CDS             1441..2307
                     /gene="ospB"
                     /locus_tag="JN749_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HAY7370225.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            2636..3376
                     /locus_tag="JN749_004032"
     CDS             2636..3376
                     /locus_tag="JN749_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7370226.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(3729..>4154)
                     /locus_tag="JN749_004033"
     CDS             complement(3729..>4154)
                     /locus_tag="JN749_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HAY7370227.1"
                     /translation="RDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGDKKADIAL
                     YLLSKFNFTKQDIAEMEKMNNNIYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKK
                     FQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRFER"
BASE COUNT         1268 a          812 c          804 g         1270 t
ORIGIN      
        1 ttcgggggaa aaacgtgtat ttttagtcat tctgtttacc tctttctcag ggcgtttagt
       61 ctccaggatt tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga cttactgttg
      121 gcattaccga ccctgagacc ggagaaataa gaaccgctta gatcatcgtg gctgttctcg
      181 gggtatcaag ttacacatgg gcagaggcta cccaaggtct actattatcg cctgaaagcg
      241 ctgatggaac agtgctatca gggacatgct gatgaaaata cctaaataat cagccagcac
      301 tctatctttc caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa attcagaaat
      361 taaggaaacg cctcttggtg cacataatct atcttatgtt aaatgaaatc taatttcttg
      421 attatatgct ggatcgttcg tgggtggctt tgcccctata tttccagaca cctgttatca
      481 ttggcccgct gccgcagata ttcccgtggc gagcgataat ccagcgcact atgcggatgc
      541 cattcgttat aatgctcgaa tgcctctgca aggttctttg ctgccgttaa cccgtcaggt
      601 ttgggcatga tactgatgta gtcacacttt attgttttca cgaagctctt tgctattctg
      661 ttactctccg gactccgcac cgccgtgttc ttcggttcaa caaagtccca acatccgggt
      721 gaactggcgt gtttcattag cccggtagca tgaaccatta tccgtcagtc actccactgg
      781 agacgacgga agatcgttgc cgaagcggtg ttccaccgct cccagcatgt cgccaatggg
      841 agagcatcta cggtctgatt gaactgttta ttcaaattgt ccaccgtctg tcagtacgct
      901 ctgaacgtcg gctggttaaa acactgctcg ctgattttca gagggttcac ggtaaaacag
      961 cacttttatt ccggattgcg gaagcggctc tgaataatcc ggatgggctg gttaaagagg
     1021 ttgtgtacca tctgcatatt gttgaaccag atcgcagcgg taagagaagt tcatcatact
     1081 tggcgcagtt acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac
     1141 cggaacaaga tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct
     1201 ccacggtact ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt
     1261 gaaattcttt cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat
     1321 ataattgcat ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat
     1381 atgaaaatat aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt
     1441 atgaatttag atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata
     1501 tcagatattg catttgcaca tataataaaa agggtaaaaa attcatcatg tactcaccca
     1561 aaagcagcat tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct
     1621 attatgggac aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt
     1681 aaaaatgaaa aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt
     1741 gaaccaatag taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta
     1801 aaaaaagcga aaataaaaac agtaaacgat actgatttta aagagtataa taaggtttat
     1861 attcttgggc acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat
     1921 gtacaaacaa tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt
     1981 tttacttcat gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct
     2041 gaaagtcttt cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta
     2101 gagcagataa aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc
     2161 ggctatcatg gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt
     2221 tcaacttcaa ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact
     2281 tttattatta ataaagaact ggattagtat aaaattttta acctatagtt aattgtttgc
     2341 atttaagatg aagttatttt atctttagta gaaaagtgct tccctatgag ggaaaatcac
     2401 aaatcatcat aatacagaga caaaacgata cgaacaaata gataaaaaac atcattcttt
     2461 taccacatta tttttcgtga atatgaaaaa taatgttatt actttaatat aagactattt
     2521 tttgtttttc catcactctg ttcaaatttt tccgcatgac ttgtgttttt tgtaacacag
     2581 ctcgtttttt acagctgacc aaaatcatca attaattatg ctaaggaaat aaattatgaa
     2641 aaccaaaaac tttcttcttt tttgtattgc tacaaatatg atttttatcc cctcagcaaa
     2701 tgctctgaag gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact
     2761 tccgccgcct ccggcagagg attcagtagt atttcaggct gacaaagctc attatgaatt
     2821 cggccgctcg ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga
     2881 gaattttggt cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc
     2941 aatcttatat cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa
     3001 cgccaaagaa tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac
     3061 acctgataaa gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt
     3121 tggttgggca gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact
     3181 tcgacgtgga tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga
     3241 tgtagaggct gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga
     3301 atttaccaaa agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa
     3361 tgaactgacc ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat
     3421 ctaccctata tgagcatagg aggagtctat gggcacacca cgttttactc ctgaatttaa
     3481 gggattactg gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc
     3541 tgccatcgga aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac
     3601 tcggggcggt tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc
     3661 ccctatatcg atcaatataa caaaaatgac aacttcagat tcagggcaca acctaaatat
     3721 attttaggtc acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaatca
     3781 atcatttttg aatctttgct tttcattgca ttgtcaagca tagtgtcccc actatttgct
     3841 ttttgaaatt ttttgtttac atcaaccaat ccattattga taaaatattc tagcacttta
     3901 tagttggcac catctttact aagtaagtat tcaacatcat acagattaca atatatattg
     3961 ttgttcattt tctccatctc tgcgatatct tgttttgtaa aattaaattt agatagtaaa
     4021 taaagtgcta tatcagcttt tttatcgcct aaagaataag caagggcctg atgagccatt
     4081 tttttatcag tcactttatt gttaatctct tcataattag atgctttaat cgcatcctcc
     4141 aaagatatgt ctct
//
LOCUS       DADLTF010000239         4075 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.243, whole genome shotgun sequence.
ACCESSION   DADLTF010000239 DADLTF010000000
VERSION     DADLTF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.243"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..113
                     /locus_tag="JN749_004034"
     CDS             <1..113
                     /locus_tag="JN749_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HAY7370228.1"
                     /translation="QAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JN749_004035"
     CDS             complement(314..1171)
                     /gene="sitD"
                     /locus_tag="JN749_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HAY7370229.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JN749_004036"
     CDS             complement(1168..2025)
                     /gene="sitC"
                     /locus_tag="JN749_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HAY7370230.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JN749_004037"
     CDS             complement(2022..2849)
                     /gene="sitB"
                     /locus_tag="JN749_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HAY7370231.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JN749_004038"
     CDS             complement(2849..3739)
                     /gene="sitA"
                     /locus_tag="JN749_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HAY7370232.1"
                     /translation="MLLGCLALTCSITFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1108 a          997 c          997 g          973 t
ORIGIN      
        1 tgcaggcgca ggtcgatcgc aacgatgaga tttatcgccg tcagcgggag ctgaaggaag
       61 agctgaatag tctgaaggat ttgaattcgg ttaggaattt tatcgtggaa taacagaagc
      121 tgcggcacgt cgtatgcagg aacgtgccgc ggttggctgg taaactttcg atagtgcgag
      181 tattgaatga tttctaggcg ttatcgattt tacgtatttt ttgcatgata gaattcgtac
      241 ctcctcccaa cgaccttcca tggctttccg gacttctgta ggctggcgga cgatgctgtc
      301 ctcggatgtt gtgctaattg ggtgataaca gatcctgtgt atgggcgttt ccctttgtgc
      361 gtgcgttgat gctggtaacg gtaaacgtca tgatgaacac gaccgcgaat aagacgacaa
      421 tcgtcggagc tggtgcgctg tcgataaaaa aggacagcca gacaccagtc attgacacta
      481 ttaccgatat gccagtcgcc agtaggagcg caatatgaaa gcgttgtgtc aggagtactg
      541 caatggcgcc aggcgcaatg agcagagaaa tagagagaat gatacctact gctttcagcg
      601 tcgccacaat ggtcagggaa accatgcaca gcagcccata atgcagccat ctcgtatgca
      661 aaccgcttac ctgcgcctgt tgataatcaa agctgaacag caaaaaatcc cgccatttta
      721 cgctaataac aagtgtaacc agcccggcaa taatcaccgt ctggattata tcgccgatgg
      781 ttatcccgag catgtcgccg aaaagaatat ggtcaagatg tacgtctggt ttaattgcaa
      841 tatacaagat cagacctgcg gcaaacatgc cagaaaaaac aatccccata actgtatctt
      901 gcttgattcg gctgttgtct ttcaggtatc ccgtcgccac cgcgcagaat actccggcga
      961 cgaatgcccc tgtagccaga ggtaacccca gaatccaggt aagcacgatg ccaggaaaaa
     1021 ccgcatgact catcgcgtca cccatcagtg cccaaccttt caggaccaga aagaccgaca
     1081 gcagcgcaca gggtatggaa actaccagtg aaatcagcag tgcgtgactc ataaaggtga
     1141 actgcagtgg ctccagtaat aatgccatca tgagcgatcc tccagcgctt tatgcgcacg
     1201 ccgacgattt gccaaaagtc catgggtggg cgcaaataca aatgctaaaa gaaacagcag
     1261 tgtctgcgcc accacgatga ttccacctgt cgcgccgtcc agaaagtaac tgacccacgc
     1321 gccgaggaaa ctggttacac tgccaatagt tacagcaatt atgagcaggc ggggaaaacg
     1381 atcggttaac aaccatgctg ttgcaccggg agtcaccacc agacagatca ccagaaaggc
     1441 accaaccgtt tgcagcgccg caacggtcga aaccgacagc agggtgaaga agaggatttt
     1501 taatctgccc ggatgtaatc caatagcacg ggcgtgattc tcgtcgaaaa aggtcaccat
     1561 aagatctttc catttgaaaa aaagcacgat gatcgagaga atgccaatga tagtgagttg
     1621 caggatatcc gccggatcga ttgccagaat attgcccagg acgatggtct gaatgttcac
     1681 ggatgtcggg ttcaatgaca ccataaacag ccccaggcca aaaaaggacg agaagatcag
     1741 gccgatgatg gcatcttctt ttaagcgggt gcgttggtta agaaacagca tgctgcccgc
     1801 tgccagtccg ccggaaaaga aggctcccag agaaaacgga agccccagca tatatgcccc
     1861 cgcaacaccc gggacaatag agtgcgacag cgcatcgcca atcagcgacc agccttttag
     1921 catcagatag catgacagga aagcgcagag accgccgacc attgccgaga cccacatcgc
     1981 attgagcata tattcatagc tgaagggttc cagtagcacg ttcatcttgc ttccctctgc
     2041 accgctgacg gtcgatgtgc cacaaaaggg cgttcatcgt cagtaataat gctttcttcc
     2101 gagccgttaa gtgtgacatg gcgcagtacg ccgctaaaag ccagctccag gttctcggct
     2161 gtaaaagtgg tgtctgtcgg cccgcttgcc aggacggtgc ctttgaccat gaccgtataa
     2221 tcgcaaaatg tcgtgactga gccgagattg tgcgttgaga caagcatcgt tttgccttcc
     2281 gcgcgtaact ccctcaacag gctgatgatt ttcgcttcgg ttttcacatc aacgccggta
     2341 aaaggttcat ccagcaggat cacatcaccc tgctgtgcaa tcgctctcgc cagaaagacg
     2401 cgtttctttt gtccaccgga aagctcgccg atttgacgat ggcgaaaatc caccatatcg
     2461 acacgttcca gcgcatcggt gacaatctga cgatccctct ttttggctat acgcaacata
     2521 cccatatgcc catagcggcc catcatcacc acatcttcca ccagtacagg aaatgaccag
     2581 tcaacctctt ctgactgagg gacgtaggca accaggtttt tctgtagcgc ctgtcgcgtg
     2641 ggaataccca gaacagatat ctttccgctg gtcagacgca caaatcccat aatcgcttta
     2701 aacaacgtcg attttccgga accgtttacc ccaaccagag cggcaataga tccacttggt
     2761 actgtgaagg atgcatcgcg cagtgctgtg tgcccgttac gccaggtgac agtaacatca
     2821 ttaacgacaa tgcctgcaga ttgcatcatt atttctccct tttcccggct ttaattccct
     2881 gtaccagcgt actggtagta accttaagaa ggtcgatata cgtaggtacc gggccgtttt
     2941 ctgtgctcag ggaatcgaca tagagcacac cgccgtagtg tgcgccggtt tcacgcgcaa
     3001 cctgacgcgc tggtttatcg gaaatcgtac tctcgctaaa gactgccggg atatgatttt
     3061 tcttaactat atcaacaacc ttacgtacct gctgcggtgt tccttgttga tcggcattaa
     3121 tcggccacag ataaagctct tttagcccca aatcgcgtgc gagataagaa aaagcccctt
     3181 cactggtgac catccatcgc tgattctcag ggagttccgt aatctgctta cgcagggggg
     3241 caagggtttg ggtaatcttg gctttataag tatcggcatt acgttggtag gtttgtgcat
     3301 ttgccggatc gtattttatc agcgcatcac gaatattatc gacgtaaatc agagcattat
     3361 ctggcgacat ccatgcatgg ggattaggtt tgccctcata gggtccttcg gtgatcccta
     3421 ctggcgtcac acccgaagag acaattactt ctggaacccc attgagatgc tggtaaaagc
     3481 gttggaacca caattccaga ttcataccat tggcgagaat cagttgtgcc ccctgcgcac
     3541 gtttaatatc gccaggggta ggctgatact catgaatttc tgcaccaggc ttggttatgg
     3601 atgagacttc tgcagcatct ccagccacgt ttttggccat atccgcgatg atggtaaatg
     3661 ttgtaatgac cttgaatttt tcagttgcac ttgcctgaaa tgtgatcgag caggtgaggg
     3721 cgaggcaccc caagagcatg gttacttttt ttattgagtg cataatttag tacctatagt
     3781 gagcaaaatt agaacaaaca tagcaacggc tatgttttat agcataacct atacaaaatg
     3841 aaaataatta tcattaacag ctttgaggcc taaaacaggg attaggttgt ttcctgctgg
     3901 cgacatgata ttgaggaaaa aagtagagca gctattaggc tttgaaatga ctttatgagg
     3961 aaaattgttg atggcaaaaa tggggcaaaa cgctgtataa ggggcaaaaa aggggcaaaa
     4021 aagagtgggt tatcgtagct tattgttgtc gctgataatg tttaacatat tgaaa
//
LOCUS       DADLTF010000240         4019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.244, whole genome shotgun sequence.
ACCESSION   DADLTF010000240 DADLTF010000000
VERSION     DADLTF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.244"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..305)
                     /locus_tag="JN749_004039"
     CDS             complement(<1..305)
                     /locus_tag="JN749_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7370233.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCV"
     gene            complement(516..1913)
                     /gene="ccp"
                     /locus_tag="JN749_004040"
     CDS             complement(516..1913)
                     /gene="ccp"
                     /locus_tag="JN749_004040"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HAY7370234.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2318..3967
                     /gene="treF"
                     /locus_tag="JN749_004041"
     CDS             2318..3967
                     /gene="treF"
                     /locus_tag="JN749_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HAY7370235.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDLYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVSREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1040 a         1010 c          936 g         1033 t
ORIGIN      
        1 tttacgcaac gcaggtcgat ggctgcggat tgcggatatt cttctttgtc gatccagttt
       61 ttattgatcg acaaatccat caatttatgg acgttttcgt cgtcccaggt ctggcagaaa
      121 gtggctaggt tctgacgagc gttgccatca agatataatt catcattgat aatctggaat
      181 gcgacatcat cgcgcatttc gtgcagcgga aatcgttttg actccgcgat agtagaaatg
      241 gcctttgcgc caaaacgtga atcgagtagt tctgagcgga aatccgttaa cagcttctgg
      301 tccatttcga actccttaaa tttatttgaa ggcaataaaa aagtaggatt tatccgcaat
      361 ggaagcaagg cattacaggc ttaatttaaa taacaaaatc ctaagcagaa aaacgacttt
      421 ttataatata aacatttaac atgataatat taaaaacaga ctttattatc gtaataaaaa
      481 tcgcccaaac attcctgttc gggcgatcaa attaattatt gtttatcctg catatacggc
      541 gtgtacaccc cgttcagact gtgcaggaaa gctacgatat catccacatc ctcctgcggc
      601 agctctttgc ctacctgata gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg
      661 tcaccgcgat ggaagtacgg tgccgttaaa gcaacgttac gtaaaccggg tactttctga
      721 cgcaatttat cacgctcttc tttagtcaca ttcatacgac caatatccgc cgccgtaatt
      781 tccccaaagt taaagtcttt tttcagcacc aacggttcaa aggaacgtcc gccgagaata
      841 ataccaccat gacaagttgc acatttatta tctttaaata attgatagcc ttttttctgt
      901 tgcgccgtca gcgcattttc atctccacgc aaccatttat caaatgggga atccggcgta
      961 attaatgttt tctcaaattc agcaatggca tcagtaatat tttcgccact gaaaccttgc
     1021 ggataaactt cgaggaactg cgctttaagc tgcggatctt tttccagctt agcaataatt
     1081 tcgtcccagg atttcgacgc catttcaatc gggttcaacg gcggtccacc tgcctgatcc
     1141 tgcaatgttg ccgcacgacc atcccagaac tgctcaacgt taaatactga gttaaatacc
     1201 gtcggcgcat taatcggccc aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg
     1261 acgccccccg cattcaacgt atggcaatga gcgcatgaaa tggtgctatc agccgataaa
     1321 cggggatcgt gatacagcgc aaagcccaac gccacttttt gcgcatcggt aggcagtttt
     1381 tgcgggatgg gctgcaccgg ttcattgcga tgctccggag cagtatcatt gctggcgtaa
     1441 tattccgcgc gctgttttgc aatccaggcc agtatttccg cccgctcttc atcactcacc
     1501 ttacccgccc agtgtagcgc ggtataacgc gttggtggca tagtttcata ctgcatcacc
     1561 cattcaatct tattcagatc gctttgcgaa acgggtttat cagccagcag tgccgcacgc
     1621 acggcctcga ggttaaaaga tttatatcca agcttaatgt cgtaatccat caactgtttc
     1681 gcgccaggaa tataataata ggcgggtaat tctgccgaag gcgtgtggca atagtcgcat
     1741 cctttttcgc ggagaaagcc taaaacctta ttattttcac tgacagcaga tgcctgaaca
     1801 tcggcctgtt tactgcgttt attatcgtgg taccacacat aaccagataa ccctaaatag
     1861 caaatcgcga cgccagccag gccgatcgcg gtaatacgtg agaccatttt cattattctt
     1921 ccctcacggt ggtgagttat tgtgacccag aaaattttcc agaatgctgc aaacgtctct
     1981 gcgagacaaa gccatcataa aaaacaggga gatacgcttt ttgataggaa tcaatcaatt
     2041 acatgaatat aaactatcaa taagatagcc tgtgcctatt acccagatca ggaatggcat
     2101 atcatgattg cggcgagagc aggattgaat gttctgaaaa tgaaaaacaa caccaaatcc
     2161 gtatttttat aaattccatt gatattagtg cgttatattt tttgacaagt taataaacgt
     2221 gatccaccgc acgctttgtc gcccaccagg cggagcgaat gactaccctt aaagaaaagc
     2281 ccgataatta gcgacgaatt tcggaggttg gatccttatg ctcaatcaga aaattcaaaa
     2341 ccctaatcca gacgaactga tgatcgaggt cgatctctgc tatgaactgg acctgtatga
     2401 attaaaactg gatgagatga tcgaggcaga accggaaccc gagatgattg aagggctgcc
     2461 cgcctctgat gcgctgacgc ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc
     2521 ggcgaaaatt ttccccgaca gtaaaacctt tcccgactgc gcacccaaaa tggacccgct
     2581 ggatatttta atccgctacc gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt
     2641 tgttgaaaat cacttctggc tgccggaggt ctactccagc gagtatgtat cggacccgca
     2701 aaattccctg aaagagcata tcgaccagct gtggccggtg ctaacccgcg aaccacagga
     2761 tcacattccg tggtcttctc tgctggcgct accgcagtca tatattgtcc cgggcggccg
     2821 ttttagcgaa acctactatt gggattccta tttcaccatg ctgggactgg cggaaagtgg
     2881 tcgggaagat ttgctgaaat gcatggccga taacttcgcc tggatgatcg aaaactacgg
     2941 tcacatcccc aacggcaacc gcacctatta tttgagccgc tcgcaaccac cggtttttgc
     3001 gctgatggtg gagttgtttg aagaagatgg tgtacgcggt gcgcgccgct atctcgacca
     3061 ccttaaaatg gaatatgcct tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc
     3121 ctatcgccat gttgtgcgga tgccggacgg atcgctgctc aaccgttact gggacgatcg
     3181 cgacacgccg cgtgacgaat cctggcttga ggacgttgaa accgcgaaac attctggtcg
     3241 cccgcccaac gaggtgtacc gcgatttacg cgcgggagcg gcctccggtt gggattactc
     3301 ttcccgttgg ctgcgtgata ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc
     3361 catcgatctg aatgccttcc tgtttaaact ggagagcgcc atcgccaaca tctcggcgct
     3421 gaaaggcgag aaagagacag aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc
     3481 ggtaaaccgt tacctctggg atgatgaaaa cggcatctac cgcgattacg actggcgacg
     3541 cgaacaactg gcgctgtttt ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa
     3601 ccatgaacag gccgatcgtc tggcaaacgc cgtacgcagc cggttactga cacctggcgg
     3661 gattttggca agcgagtacg aaaccggtga acagtgggat aaacccaacg gctgggcacc
     3721 gttacaatgg atggcaattc agggatttaa aatgtatggc gatgaccttc tgggtgatga
     3781 aatcgcgcgc agttggctga aaacggtgaa tcagttctat ctggaacagc acaaaatgat
     3841 cgagaaatac catattgccg atggtgtttc ccgcgaaggc ggcggtggcg agtatccgtt
     3901 gcaggatggg tttggctgga ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga
     3961 accataatat ttttacagcc agccgctaac ttcctgctgg ctgtaaaatt atcctcttc
//
LOCUS       DADLTF010000241         3955 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.246, whole genome shotgun sequence.
ACCESSION   DADLTF010000241 DADLTF010000000
VERSION     DADLTF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.246"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(434..640)
                     /locus_tag="JN749_004042"
     CDS             complement(434..640)
                     /locus_tag="JN749_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HAY7370236.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            953..1042
                     /gene="kdpF"
                     /locus_tag="JN749_004043"
     CDS             953..1042
                     /gene="kdpF"
                     /locus_tag="JN749_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HAY7370237.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1042..2715
                     /gene="kdpA"
                     /locus_tag="JN749_004044"
                     /pseudo
     CDS             1042..2715
                     /gene="kdpA"
                     /locus_tag="JN749_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
     gene            2738..>3955
                     /gene="kdpB"
                     /locus_tag="JN749_004045"
     CDS             2738..>3955
                     /gene="kdpB"
                     /locus_tag="JN749_004045"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HAY7370238.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIE"
BASE COUNT          869 a          953 c         1081 g         1052 t
ORIGIN      
        1 ataacatctt attggattag atatgttaac aattaattat tcttaatata tcaataaagt
       61 atatattact ctctttacta tctgttttta ttgcggtagc gatactaata acaaaattcg
      121 gaatttattt agatgctgga attagaaaaa gggatgacgg ctttctcacc ttaccctgaa
      181 gtttcacgaa agtgtgtaag gaagtatgca atctaactgg agatttctga tgatctagtc
      241 gcttagagcg acaatgaagg tgaccgcatc ctgggtagcc ctatgaaagg atactattga
      301 aacgtgatgc taaaacagtg atgaaatgaa taatacgatg gaaacgtctt ccatgttaat
      361 tatcatggaa gacgttgatg ttgaacacct aaaccagccc cctgatacga ggctggctta
      421 agcgcgcagg caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc atccacaccg
      481 gtttatcgct ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg gcgcggtctt
      541 tccagaaaaa catgaaaggc agcgccagaa cgcccgctgc aaccgcatat gtacggcgta
      601 aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc cccttttgta
      661 tctacccggt gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa tagtaccgcc
      721 ttttgtgtaa ttttaccact catccgacca cttatttttg cttattgatg gtttatttac
      781 attcacactg taattaaatt acacaaaagt taaattaata ctaaccatca gttaaatcat
      841 ggcctttgcc atttttatac tttttttaca ccccgtccgc agatttttgc gaaatctttg
      901 cagccagaat tctactcttc cggtatcact tttaggctac tggaggtgca ctgtgagtgc
      961 aggcgtgata gccggcgtgt tgctggtgtt tttattactg ggttatttgg tttatgccct
     1021 gatcaatgcg gaggcgttct gatggctgcg caagggttct tactgattgc cacgttttta
     1081 ctggtattaa tggtgctggc gcgtccttta ggcagcgggc tggcgcggct gattaatgac
     1141 attcctcttc caggtacagc gggcgttgaa cgtatactct ttcgtttacc tggcgtctct
     1201 gaccatgaga tgaactggaa gcaatatctt tgtgccattc tcggcctgaa catgctgggg
     1261 ctggcggtgc tgttttttat gttgctcggt cagcactatc tgccgcttaa tccatagcag
     1321 ttgccggggc tgtcgtggga tctggcgctg aataccgccg tcagctttgt cacgaatacc
     1381 aactggcaat cttatagcgg tgaaaccacg ttgagctatt tcagccagat ggcaggctta
     1441 acggtacaaa actttctttc tgccgccagc gggattgcgg tgatttttgc cttcatccgt
     1501 gcgtttactc gccagagcat gagcacactc gggaatgcct gggtcgatct gctacgcatc
     1561 acgttatggg tgctagtccc tgtggcgttg ttaatcgcac tgttttttat tcaacaaggt
     1621 gcgctgcaaa actttctgcc ctatcaggca gtgaataccg ttgaaggagc gcaacagctg
     1681 ttacccatgg gaccagtagc ttctcaggaa gcgatcaaga tgctcggtac taacggcggt
     1741 ggcttcttta atgccaactc gtcgcatccg tttgaaaacc caaccgcact gaccaacttc
     1801 gtgcagatgc tggcgatctt cttgatccca acagcgctgt gctttgcctt tggtgaagtg
     1861 acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat ttttgtcatc
     1921 tgcgtaggcg tggttatgtg ggcagaagtt cagggtaatc ctcatctgct ggcgctcggc
     1981 gcagacagca gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct ggtcagtagc
     2041 ctgtttgcgg tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat gcatgattcg
     2101 tttaccgctc tcggcggcat ggtgccgatg tggctaatgc aaattggtga agtggtgttc
     2161 ggcggtgtcg gttctggtct ttacggcatg atgctgtttg tcctgctggc ggtgtttatt
     2221 gccgggctga tgattggtcg tacaccggaa tatctgggta aaaaaatcga cgtacgcgag
     2281 atgaaactga ctgcactggc gattctggtt actccaacac tggtactgat gggcgcggcg
     2341 ttggcgatga tgaccgacgc cggacgtagc gccatactta accctagccc gcatggtttt
     2401 agcgaagtgc tgtacgccgt gtcgtccgcc gctaacaaca acggcagcgc ctttgccgga
     2461 ttaagcgcca actctccgtt ctggaactgt ttactggcgt tctgcatgtt tgtcggtcgc
     2521 tccggggtga ttatcccggt gatggcaatt gccggttcgc tggtgagtaa aaagagccaa
     2581 cccgccagct ccggcacgct gccaacgcac ggcccgctgt ttgttggcct gttaatcggc
     2641 accgtgttgc tggttggcgc actgaccttt atccctgccc tggcgcttgg tccggtggcg
     2701 gaatatctct cctgatgata ttgagtgagc actgaatatg agtcgtaaac aactggcgct
     2761 attcgaacca acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat taaacccgca
     2821 ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc tgaccacctg
     2881 tattagcatc gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta gcgcggcaat
     2941 tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc tggcagaagg
     3001 ccgcagtaaa gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct ttgcccgcaa
     3061 gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc aactgcgtaa
     3121 aggtgacatc gtgctggtag aagctggcga tattatcccc tgcgatggtg aagtcattga
     3181 aggcggcgca tcggtcgatg aaagcgccat caccggggaa tcggcaccgg tgatccgtga
     3241 atccggcggc gattttgcct ccgtcaccgg cggcacgcgt attctttctg actggctggt
     3301 gattgagtgt agcgttaacc ccggtgagac atttctggat cggatgatcg cgatggtgga
     3361 aggcgcacag cgacgcaaaa caccgaacga gattgccctg actatcctgc tgattgctct
     3421 gaccatcgtc tttttactgg caaccgccac gctgtggccg ttttccgcgt ggggcggtaa
     3481 tgccgtgagt gtaacggtac tggtggcgct gctggtctgt ctgatcccaa ccactattgg
     3541 tggcctgttg tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg cgaatgtgat
     3601 tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc tactggataa
     3661 aaccggcacc atcacactcg gtaaccgtca ggcgtcggag tttatccccg cgcagggcgt
     3721 ggatgaaaaa acgctggctg acgccgcaca actggcttcg ctggctgatg aaacgccgga
     3781 aggtcgcagt attgtgatcc tcgccaagca acgttttaac ctgcgcgagc gcgatgtgca
     3841 gtcgctccat gccacctttg taccgtttac tgcgcaaagc cggatgagcg ggatcaacat
     3901 cgacaaccgc atgatccgta aaggttctgt cgatgccatt cgccgccata ttgag
//
LOCUS       DADLTF010000242         3873 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.247, whole genome shotgun sequence.
ACCESSION   DADLTF010000242 DADLTF010000000
VERSION     DADLTF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.247"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..281)
                     /locus_tag="JN749_004046"
     CDS             complement(<1..281)
                     /locus_tag="JN749_004046"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308157.1"
                     /note="catalyzes the interconversion of bicarbonate and
                     carbon dioxide; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HAY7370239.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHI"
     gene            390..1316
                     /locus_tag="JN749_004047"
     CDS             390..1316
                     /locus_tag="JN749_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAY7370240.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            1313..2083
                     /locus_tag="JN749_004048"
     CDS             1313..2083
                     /locus_tag="JN749_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAY7370241.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            2188..2628
                     /locus_tag="JN749_004049"
     CDS             2188..2628
                     /locus_tag="JN749_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAY7370242.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            2692..>3873
                     /locus_tag="JN749_004050"
     CDS             2692..>3873
                     /locus_tag="JN749_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAY7370243.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TD"
BASE COUNT          948 a          877 c         1011 g         1037 t
ORIGIN      
        1 ataatgtgtt caacttcgag tacatccact gcatactgaa ccacggaaag gcagttcagg
       61 tcggtgtgaa tgaccaggtt agcaacatta cggtgaacaa agagttcgcc cggctcaaga
      121 ccggttaaac gttctgcagg aacgcgactg tcggaacatc caatccatag aaagcgcggt
      181 ttttgcgctt gtgccagttt ctcaaaaaac ccgggatcct cttccaccag catttttgac
      241 catagtgcat tgttgctgat gagtgtatct atgtctttca tggaggttaa cgacctgtaa
      301 ccaaataatt acgtttggct aatatagggc aactccggga cgatttaaac cacagataaa
      361 gtgtaagaac gtaaggtaag taaaaattta tgaccattgc actggaactt caacagctta
      421 aaaaaaacta tccaggcggc gttcaggcgc ttcgtggaat agatttgcag gtcgaagcgg
      481 gtgattttta tgcgcttctc gggccgaacg gggccgggaa atcgaccact atcggtatta
      541 tcagctctct ggtaaataaa acctccgggc gggtcagcgt atttggttac gatctcgaga
      601 aagatgtcgt gaacgctaaa cgtcagttgg gactggtgcc gcaggaattt aacttcaacc
      661 cgtttgaaac cgtgcagcaa attgtggtga atcaggcagg gtactacggt gtggagcgca
      721 aagaggcgta catccgcagc gaaaaatatc ttaagcagct cgatttatgg ggaaaacgca
      781 acgaacgtgc gcgtatgtta tctggcggga tgaagcgccg tttaatgatt gcccgtgcgt
      841 taatgcatga acctaaacta ctgattctcg acgaaccgac cgcaggcgtg gatattgaac
      901 ttcgccgctc aatgtggggc tttttgaagg atttaaacga caaaggcacc accatcattc
      961 ttaccacaca ctacctggaa gaagcagaaa tgctgtgtct caatatcggc attattcaac
     1021 atggtgagct ggtggaaaat acctcgatga aggcgctgct ggcgaagctg aaatcggaaa
     1081 cctttattct cgatctcgca ccgaaaagtc cgttaccgaa gctcgatggc tatcagtatc
     1141 gactggtcga tactgcgacg ctggaagttg aagtgctacg tgagcagggg atcaacagcg
     1201 tatttacgca gttaagtgag cagggcattc aggtattaag tatgcgtaac aaagctaacc
     1261 gtctggaaga gctgtttgtt tcactggtta atgaaaaaca aggagatcgc gcatgatgca
     1321 tctttactgg gtggcgctaa aaagcatctg ggcgaaagag atccatcgct ttatgcgtat
     1381 ctgggtgcag acgctggtgc cgccagtcat caccatgacc ctttacttta ttatcttcgg
     1441 taacctgata ggttcgcgta ttggcgatat gcatggcttc agctatatgc agttcatcgt
     1501 accggggctg atcatgatgt cggtgatcac caacgcctac gctaacgtcg cgtcatcatt
     1561 ttttggtgcc aagttccagc gtaatattga agagctgctg gtagcgccgg ttccgactca
     1621 cgtcattatt gccggatatg tcggcggtgg cgtggcgcgt ggtctgtttg ttggcattct
     1681 ggtgacggca atttcactgt tttttgtgcc gtttcaggtg cattcgtggg tattcgttgc
     1741 cttaaccctg gtgctcacgg cggtgctatt ctcccttgcg ggtttgctga acggcgtgtt
     1801 tgccaaaacg ttcgatgaca tcagcctggt gccaaccttt gtgttaacgc cactcacgta
     1861 tttgggcggg gtcttttact cactgacttt gttgccgccg ttctggcaag ggctgtcgca
     1921 cctgaaccca atcgtttata tgatcagtgg tttccgctac ggcttcctcg gtataaatga
     1981 tgtgccgctg gtcactacct ttggcgtact ggtggtcttt attgtggcgt tttatttgat
     2041 ctgttggtcg ctgatccaac gtggacgtgg tttgcgtagc taaggctatt tcctctcctc
     2101 tggattcggg ggagaggagt tttgacggct atcacccttt atcaacaatg gtcagggtag
     2161 gctgattttc ggctaaggag gaaggcgatg ttaggttggg taattacctg tcacgatgac
     2221 cgggcgcaag agatactgga tgcgctggag aaaaaacatg gggcacttct tcagtgccgg
     2281 gccgtgaatt tctggcgcgg attaagctct aatatgctca gccgcatgat gtgcgatgct
     2341 ctgcatgaag cggactctgg tgagggtgtc atcttcttaa ccgatatagc cggagcgcca
     2401 ccgtatcgcg tggcttcatt attaagccac aaacactccc gttgcgaagt gatttctggt
     2461 gtcacgttac cgttaattga acagatgatg gcttgccgtg aaaccatgac cagttcagag
     2521 tttcgtgagt gtattgtcga actgggtggg ccggaggtga gtagtctttg gcaccaacaa
     2581 caaaaaaatc cgcctttcgt cctcaaacat aatttgtatg agtattaacc cgcgattctg
     2641 atggcgcttt tgctacaata aaagcgttgt ttcaccctcg gttatttttt catgtataaa
     2701 caagctgtta ttctcctgct gatgctgttt accgcaagtg tcagtgccgc gttacctgcc
     2761 cgttatatgc aaaccatcga aaatgctgcg gtctgggcgc aaattggtga caagatggtg
     2821 accgtgggga atattcgggc cggacaaatc attgccgtag agcccactgc cgcaagttat
     2881 tacgcattta attttggctt tggtaaaggg tttatcgata aagggcatct cgagccagtt
     2941 caggggcgac aaaaagttga agacggtttg ggtgacctta acaagccgct gagtaatcag
     3001 aacttaatta cctggaaaga tacgccggtc tacaacgcgc cgagtgtggg cagtgcgcca
     3061 tttggggtac tggcggacaa tttgcgctac ccgattttgc ataaactgaa agacaggtta
     3121 aatcaaacct ggtatcagat ccgtattggc gatcgactgg cctatatcag cgcgctggat
     3181 gcccaacccg ataatggcct gccggtgcta acttatcacc atattctgcg cgacgaagaa
     3241 aacacccgtt ttcgccatac ttcgacgacc acctcggtac gcgctttcaa taaccaaatg
     3301 gcctggctgc gcgacagggg atacgcgaca ctgagcatgg ctcagctgga aggttacgtg
     3361 aagaatacga tcaatctccc tgcgcgagcg gtggtgatta cctttgatga tggcctcaag
     3421 tcggtgagcc gctatgcgta tcctgtgttg aaacaatatg gcatgaaggc gacggtgttt
     3481 attgttacct cacgcatcaa acgtcacccg cagaagtgga acccaaaatc gctgcaattt
     3541 atgagcgttt ctgagcttaa cgaaattcgc gatgtttttg atttccagtc acatacccat
     3601 tttttgcatc gggtagatgg ttatcgccga cccatattac tgagccgtag tgagcacaat
     3661 attctgtttg attttgcacg ttcacgccgc gctctggcgc aatttaatcc acatgtctgg
     3721 tatctttcgt atccgtttgg cggctttaat gacaaagccg tgaaggcagc aaacgatgcc
     3781 ggatttcacc tggcggtgac aaccatgaaa ggcaaagtaa aaccggggga taatccgttg
     3841 ttactaaaac gactttatat cttaagaacg gat
//
LOCUS       DADLTF010000243         3844 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.248, whole genome shotgun sequence.
ACCESSION   DADLTF010000243 DADLTF010000000
VERSION     DADLTF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.248"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1058..1498
                     /locus_tag="JN749_004051"
     CDS             1058..1498
                     /locus_tag="JN749_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370244.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(1577..3274)
                     /locus_tag="JN749_004052"
     CDS             complement(1577..3274)
                     /locus_tag="JN749_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HAY7370245.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(3594..>3844)
                     /locus_tag="JN749_004053"
     CDS             complement(3594..>3844)
                     /locus_tag="JN749_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="phosphotransferaese subunit; phosphorylates
                     dihydroxyacetone along with DhaK/DhaL; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HAY7370246.1"
                     /translation="PQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALI
                     ARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1005 a          854 c          914 g         1071 t
ORIGIN      
        1 tcctgaaaag caaaatcgat atatactttc atatatatcg ataaaaatca tgttcaatat
       61 caaagtttag ctattgaaaa catgaagatg tgacgacttc cgcaagcctg ttgaccctct
      121 ctatcattaa aaatgtattt atcaggtggc taatattatt taaatcattt aaggctaaat
      181 catttaaata tgaatgacat tattgttttt tatcgtccac ggttacgcga cgttgaagtt
      241 acttgtttta caatgagcga tttttgatat ctcgccgaga aaattatcag cattctctcg
      301 tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga atgctggggg ttatacaaaa
      361 cctatgttgt tgtcgtccgg attcaccgct tgctgatagt aagcagcgta accagaagtt
      421 accgcaacaa gactttgagg attttaagaa cgacgttatt aatgtcatta acaacaatga
      481 agatatttat tttcttagag agagtaattt actgaatgcg tttattcgaa cgtgcaaaaa
      541 aagagatgat aacagttacg gaaatatgat atttacgctt tattttgata acgatatcta
      601 tgggaacaga attatcaaag catcgctgaa cggcggagag ttgcagttat taagcacagg
      661 gtgtagcaat gataacattc tccattttat taatgcattt ctcattcggg tgaaatttcc
      721 gctacctgga cgcataaaac cggaactctc gccaaaagcc gggcactccg aaggaattga
      781 cgcgttgcgc tgctctgtaa cggcatttac cgcaagccat ttatttcgcc agcgacaatc
      841 cgcagagaac caaacgcatc aggaaacgac tgaacacaat aaatactgta ttgatcaaca
      901 tattatactg gcacctccag cacgcagtga ggatacactg tatcatcagc gaatcttatc
      961 actgcggagc tggttgaacg actttctgag cgaaataagt cggttcccgg taacaacaac
     1021 agaatccttt tttaattatt gtttcgttgt ttttggtgtg attcagaaaa taaatatttt
     1081 agacagtagc cagtcaggcg ttaaaaatgg ggcggatagt gaggcggttt tggctgcgct
     1141 tattgaacat ttagaattga tcaatccttc tggcagatta agccaaaata cccgttcggc
     1201 aatgcttcag ttacgcgaag aggaatggtc tgaattcttt ttttggttgc taaattctct
     1261 ggagtgtctg gattatgtga ttattaattt aactccagaa agcaaaaaaa cgttgatgag
     1321 tgaacaccgt aacaatattc aggtagcaat tgatgcttta tatagccaac gtcggcgtaa
     1381 atcgccgggt gatgaaagtg aaacacttac ccgtagaaat gacgctatct tcggcaacca
     1441 cgtatggcaa acatttgcgc aatacttccc gccaggactt gaaaaaccgt ctgtgtagaa
     1501 aaaaccatag atatggtgtt atctgccgac aacgccatcc ggagaagccg aatgacgttg
     1561 tcggagtaag cgctggttaa ggtgtgggtt gtgcctcttt ggttgagggt tgcgtcgttg
     1621 ctgacttaac ggtcggacgc gtcgccggaa cattgtcaca cggttgctct ttcgggcaga
     1681 tcaaatccag cattttcagc gtcacgccat tggtccagcc aaagccatcc tgtaatggat
     1741 attcgccacc gccgcccccc gttccggtgg tgctgacatc atatttttcc accagctttt
     1801 tctcccggtc ataggtgtgc tgaacattgg tcaggaagtg ccagctaatg tccatcgcca
     1861 cctctttttg cccgtagttt tgtaatcctt ctgtcgcgac ccactgtaac ggtgcccagc
     1921 catttggcgc atcccattgt tgcccacttt tcaccgacgt ggtgttcagg ccgccgggtt
     1981 gcagcagatg tgttttcgtc gccgtcgcca ttttgttggc gcgatctttc gctgccgcat
     2041 tgacgtacag cgggaacagg gcggccgcgg ttaactgatt gcgcacttta tgacttttca
     2101 ggtcgtaatc ggcataccag ccttgttgat cgttccacag gtatttttcg atcccttttt
     2161 gacgggcatt cgccagcgtt tcgtactggt ttgccatcgc gttatctcct gcagctttgc
     2221 tggcgcgggc gaggattttt tccattttaa acatcaggct gttcagatcg accggtacga
     2281 tgctggtggt gcgtaaggta tttaactgct gcgggttgtc catccagcgc gagctgaaat
     2341 cccagccaga cgcagcggca gagcgcaggt cgcggtaaat ttcagtggca ggtcgattcg
     2401 gattgctttt ggcggtggca atatcttcca cccatgactc tggtcgtggc gtatcgcgat
     2461 cgtcccagta gcggttgaga agggtaccat cctgaagttt tacaacgcgt ttttcctgtt
     2521 gtccggcttg caggttttca acaccgtcca tccagtaagc atattctttt tgcatttgcg
     2581 gcaggtattg cttcaacgcg gcatcgcctt catgctgcgc cagtaactct accatcaggg
     2641 caaagaaggg cggttgcgag cggcttaaat agtaactgcg gttgccgttg ggaatatgac
     2701 cgtaagtgtc tatttcatga gcaaaattgg ccaccatatc cgcgacttta tcccagtgac
     2761 cgctttcggc aagtcctaac atggtgaagt aactgtccca gtaatatacc tcgcgaaaac
     2821 gtccgcccgg cacgacataa ggttccggca gcggtaacag agaatcccat ttttcggtgt
     2881 tttcggtaga acgcgttaat accggccaaa gtccgtcaat atgttcgcgc agtgactgcc
     2941 cctctggcgg aacatatttc tcgccttctt tcggcagggt gaaattgacg ttaacgaaat
     3001 ggcgcagatc aaatccgctc tggttttgct gcatccgata atcagcaagg atcatcagcg
     3061 gatcgctgtt cggcacggca tcggcaaagg ttttttggtc cggaaaaagt ttggcgtttt
     3121 gcacatcatt aaacagcggc cctaataaaa tatcaggcgg ctgtggtgtt accggtgttt
     3181 cttctgcctg caccgatagc gcagcgaaac acaaaaagat acaggctgga attaacgcca
     3241 ttttttgcgg gcgagaaggt gcgggggatt tcatcaatca ttctcctttg gcgaaaccga
     3301 ataaaacggt attcaagtat cagaaaacct tagttcagga tcgcactagc tgcatgatcg
     3361 aaatctcatt ctgcgaacgg tcaggcattt tagaaaaggg aattaaaggg ataaataacg
     3421 gcgaaataga gtgatcaaat aacgtctgtt tattagccag ccagcgacat ttggttcgcg
     3481 attagcgaag aaaaatcatc ataaattggt aaaaaatata acaggtagtt aaaaaacatc
     3541 agtgctgggt aaattgccgg atgacatcag aacgatgcca tccgaacggt ggcttaaccc
     3601 tgacggttga aacgttgcgt tttaacgtcc agcgttagcg tttcttctgg ttgtatcgca
     3661 tacagtttct caccctgctg gcaaatccag ccaatcccca gttcacgggc gattagggcg
     3721 ctgtgggata ccggacttcc ggcgctaagg cagatacctt ttacaaccgc cggatccagt
     3781 tgcagtactg tggaaggata aatgttctcc gccagtagaa tagttggcga gttaaactgc
     3841 ggga
//
LOCUS       DADLTF010000244         3808 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.249, whole genome shotgun sequence.
ACCESSION   DADLTF010000244 DADLTF010000000
VERSION     DADLTF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.249"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..314
                     /locus_tag="JN749_004054"
     CDS             <1..314
                     /locus_tag="JN749_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370247.1"
                     /translation="GSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQLEGL
                     GMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGGGGA
                     H"
     gene            complement(378..893)
                     /gene="luxS"
                     /locus_tag="JN749_004055"
     CDS             complement(378..893)
                     /gene="luxS"
                     /locus_tag="JN749_004055"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAY7370248.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(1043..2599)
                     /gene="gshA"
                     /locus_tag="JN749_004056"
     CDS             complement(1043..2599)
                     /gene="gshA"
                     /locus_tag="JN749_004056"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HAY7370249.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(2672..3100)
                     /locus_tag="JN749_004057"
                     /pseudo
     CDS             complement(2672..3100)
                     /locus_tag="JN749_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(3097..3663)
                     /gene="yqaB"
                     /locus_tag="JN749_004058"
     CDS             complement(3097..3663)
                     /gene="yqaB"
                     /locus_tag="JN749_004058"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HAY7370250.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT          955 a         1017 c          897 g          939 t
ORIGIN      
        1 cggggtctat cggcacgtcg ataaccacga ccatgtggac caaccgcgag tcgatgcacc
       61 atgcgcagtt gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc
      121 aactggaagg gcttgggatg acgcaacagc aggcatcagg ctggattgcc cagcagatca
      181 ccaatcaggg gctgattatt tccgccaatg agatcttctg gatgtcagcc gggatattcc
      241 tcgtactgct ggggctggtg tggtttgcta aaccgccatt tggcgcaggt ggcggcggag
      301 gcggtgcgca ctaagtacaa ctaagccagt tcatttgaac tggctttttt caattaattg
      361 tgaagatagt ttactgacta gatgtgcagt tcctgcaact tctctttcgg cagtgccagt
      421 tcttcgttgc tgttgatgcg tacgtcacgt tccagaatgc tacgcgcaat atcctgcgct
      481 tcctgcaacg agtgcatctg gtaagtgcca cactggtaga cgttcagttc cgggatctga
      541 ttctgatcct gcactttcag cacgtcttcc attgccgctt tccaggcatc agcaacacgc
      601 tgctcatctg gcgtaccaat cagactcata taaaaaccgg tgcggcagcc cattggcgag
      661 atatcgataa tctctacacc attaccgtta agatggttac gcataaaacc agcaaacagg
      721 tgctccaggg tatggatccc tctttctggc atcacttctt tgttcggcac gcagaagcgc
      781 agatcgaaca cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc cacccgaact
      841 gcaggcgctt ccatccgggt atgatcgact gtgaagctat ctaacaacgg catttagcca
      901 cctccggtaa tttttttaaa aattttctga actctttctt cccaggcgag tctgagtata
      961 tgaaagacgc gcatttgtta tcatcatccc tgaattcaga gatgaaattt tggccactca
     1021 cgagtggcct ttttcttttc tgtcaggcgt gtttttccag ccacaccgca aacggttcgg
     1081 tatcagcggt ttccatttcc tgctgacggc gttcagatgc ctcgcgctcg gctacaaaat
     1141 cctcttcgcg cagaatttcc agcggctctt cacgcagcag attacggtag gcttctgcaa
     1201 acgctttgcc tgttccgcca ataccagtat caatcataga ccttaagata cgggcagaga
     1261 aagtcagatc gggattatcg aagcaggcaa ccagttcatc acacactttc tgatacgctt
     1321 cgccgccgtt aatgctatcc agcgtttgcg cgacgcgttt cagatcgtgg aacagatctt
     1381 tacccacctg cagtaacggg aactgcgcgg tttcgcagcc gatacccagc gtcaggcccg
     1441 gtttgcgacc ttcaaggatc acccggttcc agttaacgcg cgtacaggca agttcgctac
     1501 tgctcatttc cggtgcatca gccagcgcac accagaccat aaacaggtcg aggaatcgca
     1561 cctgctgttc atctacacca atcggcgaga acgggttgat gtccagcgaa cgcacttcaa
     1621 tatattcaat gccgccacgt aacagcgcat cagaaggtga ctcgccgctg cgggtaacgc
     1681 gttttggacg aatcggcgca tacagttcgt tttcaatctg cagcacgttg ctattgattt
     1741 gcagcctctt accgtctttc tcaataccaa tcttcgcgta ctcttccgat ggcgttttga
     1801 ttgcctgttt aaggcccgct acgtactcgt aaagatcgtt gaaggtaata ccaagattgc
     1861 tttgcgattt attggtatag ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt
     1921 aatacatacc gcactcggtt ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag
     1981 aagaacaaat cgccggagat gcaccaaaca gataaggaat gacccaaccg aaacgatagt
     2041 aattgcggat aacgcggaaa tagcccgcag aaattttctc tttggcatca gcgcccgaga
     2101 tatcaccgca cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg
     2161 aaatggtttg catcagcgcg ccgtagcgat ttttcagccc ttcacgatac agcgttttaa
     2221 agcgtccggt gttagaagtg ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt
     2281 agcatggcat acttaacggc cacatccgct catcgcccat attgcgcgcc gtataacgat
     2341 gcagatcgcg cataaaggtc agcatatgtt caatatcacc atccactggt gtaatgaatt
     2401 ccagcaatgc ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg
     2461 cttcaggatg acctgttgtt gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct
     2521 ccagcccacg ctgtatcccc tttaacgcct gaggatgttt ttccagccag gccagcgcct
     2581 gtgatacgtc cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa ttagcataac
     2641 tgtaatggtg accatatgtg caggcctaca attagtgcca ccacatcatg ccctgaacgg
     2701 tcgctgctgc aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa aagattactg
     2761 gcccccagga gatgcgcatc catcccgcta acagacacag taaatcgcca accacgggca
     2821 tccagcttaa taatagcgtg actgcaccat agcgtttcag ccagccggta gctttctctt
     2881 gccagcgcga tgttttacgc aatggaaaga aacgcccaag gataacgtta gttaaccctc
     2941 caaggctatt acccattgtt gctgttaaaa ctaaaaccta gggatgactg atcccggaaa
     3001 gcaacattgc caccagcacg acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac
     3061 tactggcaaa caacgagaaa agcgataacg cttcactcac agcaagcgaa catccacagc
     3121 gtccatgcct gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa agaccacaca
     3181 ctgcgtcggt tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt
     3241 atggtgtttg acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta atcccaggtg
     3301 cgccagcaat gcctctgcga tggcgctttc actccccgtt cctacagcca ttgggcgacg
     3361 accatgccaa cttttcacca cttcaacaag aggaagcggc tcgacactat ccagcagcat
     3421 acttcttact gcttctgttt tttcacgcgc taacgcatgc gggtcgagat cggcctgatt
     3481 cagctcaata attgcctgag caatacgcca ggtgggcgat ccattaagcg caatcatcgc
     3541 ctgaatatcg tactgaagac cgtagtgccc taatacttcg cgccacgctt tacggtgcgt
     3601 aggctccgta tccaggattg tgccatccat atcaaaaatt aaacctgcat aacgctcgta
     3661 catggtcttc tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc ttgattttgt
     3721 cgctgatggc gaaggagaag aaaattcagg aaatggataa agtagttatt tagaaatgag
     3781 atatttttga aggaaatttt ttggagaa
//
LOCUS       DADLTF010000245         3794 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.250, whole genome shotgun sequence.
ACCESSION   DADLTF010000245 DADLTF010000000
VERSION     DADLTF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.250"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            108..2105
                     /locus_tag="JN749_004059"
     CDS             108..2105
                     /locus_tag="JN749_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HAY7370251.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            2168..3445
                     /locus_tag="JN749_004060"
     CDS             2168..3445
                     /locus_tag="JN749_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HAY7370252.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          915 a          872 c          954 g         1053 t
ORIGIN      
        1 ttccaccatg cattacgatt aacgccgcat agccagttga actttgctac tttgtgagag
       61 gtagtacctt ctatccagtg cgaatttaat taatggaata aatgattatg agtgaaaatg
      121 atacaatccc aaagaagtct acaagtcaga ttaacaaagc ggtattcttt acatctgctt
      181 tgctaatttt ccttcttgtc gcctttgccg ccgtattccc ggatgtcgcc gacaaaaatt
      241 ttaaactact tcagcaacaa atcttcacga atgccagctg gttctacatc cttgctgtgg
      301 ccctgatttt actgagtgtc acgttccttg gactctcacg ctacggtgat atcaagctgg
      361 gcccggacca tgcgcagcct gatttcagct accactcctg gtttgcgatg cttttttcgg
      421 cagggatggg gatcggcctg atgttctttg gcgttgccga acctgtaatg cattatcttt
      481 cgccacccgt tggcactcca gaaaccgttg cggcagctaa ggaagcaatg cgtctgacct
      541 ttttccactg gggactgcac gcatgggcaa tttatgccat tgtggcgctg attctggcgt
      601 tcttcagtta ccgtcacggt ctgcctttaa ctctgcgctc cgcactctat cccattattg
      661 gcgatcgcat atacggacct gtaggacatg cggttgatat tttcgctgtt ataggcacgg
      721 tctttggcgt tgcgacatca ctgggttacg gtgttttgca ggtgaatgcc ggtttgaacc
      781 atcttttcgg ggtgcccatc aatgaaacgg tgcaggtaat tctgatcgtg gtcatcacgg
      841 ggttagcgac gatttcagtg gtgtccggtc tggataaggg aatacgtatc ctgtctgaac
      901 tcaatctggg tctggctttg ttgctcctgg cgctggtcct gtgtctggga ccaaccgtgc
      961 ttctgctgaa gtcatttgtg gaaaatacgg gcggttatct ttcggaactg gtgagtaaaa
     1021 cgttcaacct ttacgcgtat gagcccaagt cgagcaactg gctggggggc tggacattac
     1081 tgtactgggg atggtggctt tcatggtcgc cgtttgtggg gatgttcatc gcacgggtct
     1141 cccgcgggcg aaccattcgc gagtttgtca ccggcgtgct gtttgttccc gcgggtttta
     1201 cgctaatgtg gatgacggtg tttggtaaca gcgcgatcta tctcattatg aaccaggggg
     1261 ccacagacct cgccaatact gttcagcagg atgtggcgct ggccctgttt aatttcctgg
     1321 agcatttccc gttctcttct gtgctgtcat tcattgcaat ggcgatggtc atcgtcttct
     1381 ttgtaacgtc tgctgattcg ggggcaatgg ttgtggatac tctggcatca ggtggagtgg
     1441 caaacacacc cgtctggcag cgaatattct gggcctcgct catgggcatt gttgcaattg
     1501 cgcttctcct tgccggaggg ctaagtgcgc tgcaaacggt gacaatagcg agtgcattgc
     1561 ccttctcagt gatcttacta atatctatat acggactttt aaaagctttg cgccgggatt
     1621 tgaccaagcg tgaaagcctg agcatggcga caattgctcc tacggctgca cgtaacccaa
     1681 ttccttggca gagaaggtta cgcaatatcg cgtatctgcc gaagcgatct cttgtgaaac
     1741 gttttatgga cgacgttatc cagcctgcca tgacgctggt tcaggaggaa ctgaacaagc
     1801 aggggacgat aagccacatt agtgatgcag tcgacgatcg tattcgtctt gaagtcgatt
     1861 tgggcaacga gctgaatttt atatatgaag tgaggcttcg cgggtatatc tcaccgacct
     1921 tcgcgctcgc cgcaatggat aatgatgagc agcagactga acaacatcga tattatcgcg
     1981 ctgaggttta tctcaaagaa ggcggtcaaa attatgatgt gatgggctgg aaccaggaac
     2041 agctgattaa tgacatactg gaccagtacg aaaaacacct gcacttcctg cacctggttc
     2101 gttaatagca acatgccgtc ctgggggcgg caattattat cccggccgca atatgaggga
     2161 atgcagaatg atttcacgct ggaaatggat gctgaagcag acaattaaaa aactatggtt
     2221 cagggcaacg ttattcgcaa ttgtcgcgat aataacggcc cttttatcaa ttctttttaa
     2281 atcaatgata cctgagtcgg tttccgtgaa ggttggtgcg gaagcagtcg ataacattct
     2341 gaacatactg gcatcgagta tgctggcagt gaccacattt tcgctgagta tcatggtcac
     2401 agcctacggt tcagccacta ctaatgtgac tcccagagct acgcgtttag ttgttgaaga
     2461 cgtcaccaca caaaatgtac tggccacctt catcggttct tttctcttca gtctggtagg
     2521 gattattgcc ctcaatatgg gagcttatgg agaaaggggg agagtcattt tattcattgt
     2581 cacactggtt gtcattgcct taatcctcat cacattgctt cgctggatac agcatttgac
     2641 ctctctgggg agggttggtg agacaacggc acaagtagaa caggcggcca tcgaaacatt
     2701 tattgcgaga gcaagaaatc cctgtctcgg tggatatcca tggcttgaga acaatgaaca
     2761 gccgaaagga acggttgcag tttatccgaa gaagattggc tatgttgaat atattgatat
     2821 ggtgaaactt agcaagctgc tgaccaatga tccccgtcat gtatacctcg tggcgcagcc
     2881 aggcagtttc atacatccgt ccatgccagt tttgtacctg agtcagggcc aggagtcgtc
     2941 aatcaacacc gatttacttg agacgattat tgtctcggat gcacgttcat ttgctcagga
     3001 tcctcgattt tgtcttagcg tcatggccga aatagcctgc cgggcccttt cccctgcagt
     3061 gaacgatcct ggaaccgcca ttgatgtcat tggcagaggt gttcgtatac tttccactta
     3121 cgcgcagaat aaatctgatg aaatagaagt gaaatatcct tcagtacatg ttgcaccact
     3181 tcagaataac gatctactgg aagacttttt ctcacctgtc gcgcgcgatg gtgccagtat
     3241 gagggagatt cagataagag tccttaaagg actgtcgatg ctgagtaaag gttggcctga
     3301 gatatttgct gaagccgcac ataccctggc atttgaaaca ttagagcatg caactcgtgc
     3361 tgaccatata gattctgata gatatctaat aaaatcaact tattataatt tatttagtgg
     3421 cgaatattct aataaaaaaa catagctgca gagcaagcaa ttcggttgtt ggcaagtcaa
     3481 gggcaaagca ttcgcgtgag aatgggcgta tgctttgtgc caaaagcaaa cgttgctaat
     3541 tctgcactgt tcagcgatgg cgcagtagag acaggcactg ccactgtcca gtcggcaaaa
     3601 gcgaacactt tgccactgcg gggataaact gattgaagct ctcgcgcaaa atcacttatt
     3661 cttttgcgtt aataaaatgt aggagatggg tcattgatcc tacccacgta atatggacac
     3721 aggcctaagc gaggttcttg ttttcaaatt gttccggact gaggccgcca caccaactgt
     3781 gccgccgcca ccga
//
LOCUS       DADLTF010000246         3736 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.251, whole genome shotgun sequence.
ACCESSION   DADLTF010000246 DADLTF010000000
VERSION     DADLTF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.251"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            190..447
                     /locus_tag="JN749_004061"
                     /pseudo
     CDS             190..447
                     /locus_tag="JN749_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JN749_004062"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="JN749_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HAY7370253.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHKQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /locus_tag="JN749_004063"
     CDS             complement(1599..2351)
                     /locus_tag="JN749_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HAY7370254.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALALLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JN749_004064"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="JN749_004064"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAY7370255.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="JN749_004065"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="JN749_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3605..>3736)
                     /locus_tag="JN749_004066"
     CDS             complement(3605..>3736)
                     /locus_tag="JN749_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7370256.1"
                     /translation="AHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
BASE COUNT          966 a          886 c          889 g          995 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgcttgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagca gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttagcgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggc
//
LOCUS       DADLTF010000247         3728 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.252, whole genome shotgun sequence.
ACCESSION   DADLTF010000247 DADLTF010000000
VERSION     DADLTF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.252"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(9..389)
                     /gene="panD"
                     /locus_tag="JN749_004067"
     CDS             complement(9..389)
                     /gene="panD"
                     /locus_tag="JN749_004067"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HAY7370257.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            663..1565
                     /locus_tag="JN749_004068"
     CDS             663..1565
                     /locus_tag="JN749_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HAY7370258.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            complement(1639..2490)
                     /gene="panC"
                     /locus_tag="JN749_004069"
     CDS             complement(1639..2490)
                     /gene="panC"
                     /locus_tag="JN749_004069"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HAY7370259.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2502..3296)
                     /gene="panB"
                     /locus_tag="JN749_004070"
     CDS             complement(2502..3296)
                     /gene="panB"
                     /locus_tag="JN749_004070"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HAY7370260.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(3409..>3728)
                     /locus_tag="JN749_004071"
     CDS             complement(3409..>3728)
                     /locus_tag="JN749_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7370261.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
BASE COUNT          900 a          915 c          963 g          950 t
ORIGIN      
        1 gataacaatc aagcaacctg taccggaatc gctttcgcgg tacgtttcat ttcattgtcg
       61 ccttcaaaat aggcgacatt gggtcgccag gtgcgagctt cttcatctgg catggtaacg
      121 aagctggcga tgatgacaat atcgccgacg ctggcgcagt gggccgccgc accgttaaca
      181 gaaataattc tcgaaccgcg ttctgccgcg atggcataag tggagaaacg cttgccgttg
      241 gtgacattcc agatatcgat ggcttcgttt tcgagaatac cggctgcgtc aagaaaatcc
      301 tggtcaatgg cgcaagaacc ttcatagtgc aggtccgcat gagtcacttt cacgcggtgg
      361 agtttgccct gcagcatcgt gcgaatcata acttctacct ttctaccctg tcgttaacga
      421 agcaggcggt gcctgctttg aggaaattct cacgcagtat tgcccgattt ttgatgagtg
      481 tctactcatc tgatggcatt tgcgtcagca gtttgcgtac cgcgccgaag cgtgccatgt
      541 aacgcctgta acgaattgat atttttatga atgctgctcg gtaaacaata aagaggtggc
      601 tgacgatagc acgccttgac tggatactgc cttcacgcaa ggccacacgg aagacaggga
      661 agatggatgc accgagtacc acaccgcatg acgcggtatt taaacaattt ttaatgcatg
      721 cggagacggc tcgcgacttt ctggagatac atttgccagt ggaattacgc gaactttgtg
      781 atctcaacac gcttcattta gagtcgggga gtttcattga agagagcctg aaaggtcaca
      841 gcacggacgt gctctattcc gtgcaaatgc agggcaatcc cggttatctg catgttgtga
      901 ttgaacacca aagcaagccg gataagaaaa tggcctttcg catgatgcgt tattctattg
      961 ctgccatgca ccggcatctg gaggctggcc acgataagct gccgctggtg gtgccgatac
     1021 tgttttatca gggcgaggcc acaccttatc cgctctcaat gtgctggttt gatatgtttt
     1081 actcgccgga gctggcgcga cgcgtctata acagtccttt cccgctggtg gatatcacca
     1141 tcacaccgga tgacgaaatc atgcaacatc ggcggattgc gattctcgaa ctactgcaaa
     1201 aacatattcg ccagcgcgac ttaatgttat tgctggagca actggtcacg ctgattgacg
     1261 aagggtacac tagcggaagt cagttagttg ccatgcaaaa ctatatgctg caacgcggtc
     1321 atactgaaca agcggatttg ttttatggtg tgctgagaga cagggaaacg ggagggaagt
     1381 ctatgatgac gctggcgcag tggtttgaag agaaagggat tgagaagggg attccgcagg
     1441 gaagacagga ggaaaggcaa gaattcgccc tgcgtcttct aagtaaagga atgtctcggg
     1501 aagacgttgc agagatggca aatttacctc ttgctgagat tgataaggta attaacctta
     1561 tttaagttac ctgtgttatg acagatgacg tggggtaaat taataactgg cgccatcagc
     1621 cgtagcgcca gttaagtatt acgccagctc gaccattttg ttgtcgatca ggcgagcatc
     1681 gccaagccag gcggctacca gaattactgc ccgtttgctg gtttcagaaa cttccagcaa
     1741 tgtgtcggca tcgcgaatct gaatatcatc ggcgcggaag cctttttcat tcagttcttg
     1801 ccccgcaatg gtaataattt catcgagatc ccgttcccca gcctgcaatt tgtcagcaat
     1861 cgaacttaaa actttgtaca gaccaggcgc aattttgcgt tgttccgccg tcagataacc
     1921 gttacgggaa cttagcgcca gaccgtcttt ggcgcgcata attggcacac cgacaatctc
     1981 aatatcgaag cccatatcgg caaccatttt gcggatcaac gccagttgct gaaaatcttt
     2041 ttcaccgaag caggcgatgt ccggctggac caggttgaac agcgtgctga caatagtcga
     2101 aacgccgcga aaatgtcccg gacggctggc accttccagc atggttgaaa ggccaggaac
     2161 gtcaacgtaa gtgtgggttt cagtaccgtt cgggtagatc tcttttaccg aaggggcgaa
     2221 aactaaatcc actttacgtt tgtttagctt ctcgcagtcc tcctgcaagg tccgtggata
     2281 acgtgccaga tcttccgggc ggtcgaactg catcgggtta acgaaaatac tgacgacgac
     2341 cacatcggcg cgggctttgg cttcgtcgac cagcttcata tggccattgt gcagattacc
     2401 catagtaggc accagcgcca cgcgcttgcc ttccatacgc aggcggcgaa tttgctgacg
     2461 cagcagcggc agggtttcga taattaacac aacgtgactc cttaatggaa actgtgttct
     2521 tcgcccggat aaacgccgga ctccacttca gccatatact gccgcacagc cgcgcggatg
     2581 tcgcccgttt cggcgaggaa atttttagca aatttaggaa tgtgaccgcc ggtaatacca
     2641 aaggcgtcgt gcatcacgag gatttgcccg tcagtgacgt tgcctgcacc aataccaata
     2701 accgggatcg ccagtgcttc ggtaatacgt tttgccagct caaccggcac gcattccagc
     2761 accagcagct gtgcccccgc ggcttctaag gctaatgcat cgctgagtag tcgatcgctc
     2821 gcttcattgc cgcgcccctg tactttgtag ccaccgaaaa tattcactga ctgtggggtt
     2881 aaacctaagt gaccacatac aggaacggca cgttcggtca gcatttttac ggtttcgacc
     2941 agccactcac cgccctcaat tttgaccata ttggcaccgg cacgcataac cgttgcggcg
     3001 ttttcaaagg cttgttccgg cgtggcatac gccataaacg gcaggtcagc caatagcagg
     3061 cagtttggtg cgccgcgacg tacggcggca gtgtggtagg cgatatcagc aacggttact
     3121 ggcagagtgg aatcgtggcc ctgaacggtc atgcccagcg agtcgcctac cagcatgacg
     3181 ttaagccctt catcagcaaa tagtttcgcg aagctgtagt cgtaagcggt gatggtcgcg
     3241 aaacgctttt tatcctgctt atatttctga agtgaagcaa tggtggtcgg tttcataacg
     3301 tatcctgata aatgatgtcg tgttgcctgg cattttatca gtcacattgg tgggggcaat
     3361 gatttatcaa ttaccgcacc gccagatgat ggcggtgcgg tggacttatt aagggtaggt
     3421 tacctggaaa gtactggtgg ctttaaactc accagcttct attggtatag ttccatcctg
     3481 ttgtagcgtg gcctggaaat ggagagggta tgttatacct gtatcttgat ccgggtaaac
     3541 tcctcctgtt gtatcgtctt tgcctctcgg gtcgtaatct ttataaacag aatttgaatc
     3601 attaggtttc aatgtcatta gaggattttt actagttgct aaaccttcta tgagtacacc
     3661 gactcctttt gctgcgtcat tacctgtgag agtattacca aggagttgcc cgttttcagt
     3721 accaacct
//
LOCUS       DADLTF010000248         3705 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.253, whole genome shotgun sequence.
ACCESSION   DADLTF010000248 DADLTF010000000
VERSION     DADLTF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.253"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..164)
                     /locus_tag="JN749_004072"
     CDS             complement(<1..164)
                     /locus_tag="JN749_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370262.1"
                     /translation="MTDITANVIVSMPSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENE"
     gene            complement(300..2141)
                     /locus_tag="JN749_004073"
     CDS             complement(300..2141)
                     /locus_tag="JN749_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7370263.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2141..3547)
                     /locus_tag="JN749_004074"
     CDS             complement(2141..3547)
                     /locus_tag="JN749_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAY7370264.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQENQAQRQKEFQQAYANAYASGDRGALRQLANQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVDPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(3557..>3705)
                     /locus_tag="JN749_004075"
     CDS             complement(3557..>3705)
                     /locus_tag="JN749_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7370265.1"
                     /translation="NKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLL
                     GSLF"
BASE COUNT          860 a          942 c          809 g         1094 t
ORIGIN      
        1 tcttcattct ctacataaac ctgaatctgg ttttcaggat ttaccgggtc agtgtcaatt
       61 tttccgatat aaattttgcc attggctacg gctttaaaag aacgagccat agtgaagagt
      121 tgcgaaggca tcgatacgat cacattggct gtaatgtctg tcatttaatt tgctccagat
      181 acaatgaatc gccgcagcgt ggctacggtg aattttgggc ataaaaaaaa ccagccgaag
      241 ctggatagtt gcgttggtta tctgtcagta gttatgtgct tatttaatta tttatctatc
      301 tattgttctg ctgtaatttg gcttgaaagt atagggcgta tcgcatttgc agcattattt
      361 aacgctcttt cataagctgc cgttcctgct ttggtgtttg ccaaacgaag aagcatattc
      421 cttgcggctt tggactcata caaacgcatc attgcaccaa aaccagcctc aagccccatt
      481 gatacaccaa gagtcgcagt tgcgccaatc gtccttatcc tgttggcttg cgattgcccc
      541 gtctgagtta ctacatttgc ggtgtctgac cttgctgttt gctgtagaac ttcatgaaga
      601 gcatcaagct ctttcatgtg ctttccagaa aaaatagtgt tgtaaatttc accgcctgac
      661 tgagatttca gcttattaac ttcagtgatg aacttggctg gagagtcacc ggccttttcc
      721 gctattttgc tgacgtaagc tgcacgcata gcatctttcc ctttatcatc cagtgcgctc
      781 cagattcgtt tcacgtcaga tggttttctg cttaatacaa cggtatttat aagttcagga
      841 ctggcttcac tgcttgcctt gttgagcttg ttagcaatgt ttttattaag caccttatta
      901 taaacgtttg cataatcgga atttgcttta aggtattttg ctgcgtctga tgcaccgagg
      961 tttttagcaa ctgcgttacg aaggtctttt gacattgcat tctctaccat attggtagct
     1021 gcttttgcct ggttggggaa gaccatagca tctccctgaa cattagatct aaatgctgtt
     1081 ctgtgctgac gcaagagatc aaacgtaaca tccaaatcag ttgcagggtt tgctaattct
     1141 tcacgtaggt tacgcaagga tgtaagcagg ctttgattgg cagacgtccc aagccgttcc
     1201 tgtcttgcga tcgctgtatt cagagcattc atggtatttg tggtatcaac tgcggcatta
     1261 cccattttat tggtgacgtc attgataaca gcgccagcgg catccttccg cccccttaac
     1321 gtggtggtaa gagatttcac cacatcatca gggttgtact caccaaaacg gtcaaaataa
     1381 ttacttacca gcttactacg cgttgcatat tgctccgctc gctttgagcc tgtcccgagc
     1441 aaagccccct cggcatcctg agttaggccg cgagtgaaag catttttcgg cgggataaca
     1501 tcagatgtca ttggtgtcac gcccatcgat tctgatgtgg caattttctt cgccacttct
     1561 ggcgcaatat caccttttat agccgttatt ccacgcccta ttccctttgc tgctgcggaa
     1621 agaacaccct gagcggcaag gttaactccg gcatttttag ctgcattttg tgcgaaatcg
     1681 cctttctgat ttgcggcctc tgccagtgat ccaatagcca tgcttcctgc cgttccaact
     1741 cctggaacta aatacccacc aattgtttca ccggcttgcg cgtaggggtc tgtcggtctg
     1801 tctactggac gataaacatc atccaaaacc ttgggtccac caagcccctg actgattgca
     1861 ttaatcagac ttgcgccgcc ctgcaatacg tcaaatggta tgtttaccag accacgacca
     1921 gcctgttctg caatttgccc tgcactttga ccaccagtga gccaatcgcc agcttgttgc
     1981 atcaatgatg gttcttcccg tgttggtgca ttattggcct gattaactgt ttgttgctga
     2041 acagcctgac cagcaaaata ctcatcaatg gcggtgccaa tatcttcggt gctcgtacca
     2101 tcaggaaagg taaatgtctt accgtttgca gttactttca tcattccacc gtaaattgaa
     2161 tgcctgattt tgaggtatat gatccaacct gattccgtgg ttctcctgaa ggtgtcgaat
     2221 cttgtgctgg cgctgcgtca gtattcattg acatataccg cttaacggca ctccccaatg
     2281 attcaccttt tttaacatcc aaccccaata tctgaccgcc attacgcgat tgtccagggt
     2341 tgccattcgc gctcatccac tcggctttaa actcattaaa ctgcgcgttt cgtcgctcaa
     2401 ggtttgccat tgcatcaagc catcttgcga ccgtctcagg gttatccatg tcagttggcg
     2461 caccctgtcg aacgatctca acgtctttat ccgttgctgg gccgggaggt aggaatttaa
     2521 gaacctgact gttaacaagg gcattttggc gaatgcgcaa atcacgcaat gttgtatcgc
     2581 ttccggtaag ttttgcgaac atgttctgtg cgttaccgaa caaacctgtc gttggttttt
     2641 ctgctctgaa ctgttgagca agcgcactca tagaattggc tgagtttgat gatgctgtag
     2701 cattgtttac agccgtctcg atgccttttt ccatgtttac tgacagctta ggtgcttcgc
     2761 taatcaactg ctgagccttt tcctgcgctt gctgcatctt aaacccgaac tcttgctgat
     2821 ccagagccaa gcgttgtgct gcgatattgt gcccagtcat tgctgactga taggaaaggt
     2881 tttgccctct cgcctgaagt gcttctccag cctgattgct gcggattgtc tctgccagcc
     2941 tgcctcggtc aatttcacga ccagccatct tgtcctgaac attgaagtaa tcaatcggac
     3001 caagagcagc cattccaagg tgatcaacaa actcaccaaa tcctgaagga ttctgctgat
     3061 acatctgagc aacgctgtta gggtcaacac cgacgcgcgc cagctcattg gcgttgtttt
     3121 gcagccatga ttgcattgct tctggagacg aggccgcaag gcgtgcgcca gccgctaagg
     3181 tgccgataga attacgctgg tcttcatcaa tgaatcccat gcctttacga acggattcaa
     3241 tctggtctgg atattgatta gccaactgac gcaaagcacc gcgatcacca gacgcataag
     3301 cattagcgta cgcctgctga aattctttct gccgctgagc ctgattttcc tgctgaaaca
     3361 cccctgcaat acctgaaagg ccttgcaaag cagtcagccc aacattgtta gcgcctgaac
     3421 gctcaatatc attgttctgc ctgataagct gaagcgtatt gccgatgtca tttacgctcg
     3481 gagcgtttga gttgacgccg ccgataccag ctaacaatcc gccatttgat ccttgccaag
     3541 tagccatgat taccccttaa aacaacgagc caagcaatcc gataccagca ccaatgccag
     3601 cgccccaagg tgttgatgtt cccaaaaggc tggcaagacc tgcaccggca atcgcaccag
     3661 atgttccgcc actaattgca gtctgaagac ttgatggttt attgg
//
LOCUS       DADLTF010000249         3687 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.254, whole genome shotgun sequence.
ACCESSION   DADLTF010000249 DADLTF010000000
VERSION     DADLTF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.254"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..222
                     /locus_tag="JN749_004076"
     CDS             <1..222
                     /locus_tag="JN749_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HAY7370266.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            268..477
                     /gene="hha"
                     /locus_tag="JN749_004077"
     CDS             268..477
                     /gene="hha"
                     /locus_tag="JN749_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HAY7370267.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            516..1106
                     /locus_tag="JN749_004078"
     CDS             516..1106
                     /locus_tag="JN749_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HAY7370268.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1346..1606
                     /locus_tag="JN749_004079"
     CDS             1346..1606
                     /locus_tag="JN749_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HAY7370269.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1830..1904
                     /locus_tag="JN749_004080"
     CDS             1830..1904
                     /locus_tag="JN749_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HAY7370270.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1897..2754
                     /gene="repA"
                     /locus_tag="JN749_004081"
     CDS             1897..2754
                     /gene="repA"
                     /locus_tag="JN749_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HAY7370271.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3454..3645
                     /locus_tag="JN749_004082"
     CDS             3454..3645
                     /locus_tag="JN749_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370272.1"
                     /translation="MTENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          992 a          901 c          932 g          862 t
ORIGIN      
        1 tttcagcgag atcgttatgg tcgtattctc gggcaggttt atgcaccaga cggaatgaat
       61 attaatcagt ttatggtccg ggccggggct gcctgggtat atgagcagta caacacagat
      121 cccgtgctac ctgtccttca gaacgaagcc agacaacaaa aacgtgggct ctggtcagat
      181 gctgatccgg ttcccccctg gatatggagg caccgtaaat aaagcgatgg caatcctatc
      241 attcatctgt tctcagcggg aataaaaatg gcaaaaacaa aacaggaatg gctgtatcag
      301 ttacggcgat gttcatcagt caatacgctg gaaaaaatta ttcataaaaa cagagacagt
      361 ttatcaaaca gtgaacgtga atcatttaat tcagcagcag accaccggct agcggaatta
      421 atcaccggaa agttatatga caggatacca aaagagatat ggaaatatgt aaggtaataa
      481 ttcagcaata attaattgcc aggagcagat aaataatgaa gttgattatt tttattctca
      541 ttgttctgat tattgcaacg ctacttatcc ggattatcct cagaagtgta aatcagcatt
      601 ctcctttgct gatgcaattg catgcggcag gtatacggac tggtgatgca gagcgaatac
      661 tgtccagcgg tgaatactgg caacgtcaga agacgctgct gacagaaagg gaagtcagtt
      721 ttatgaaagg actgttcaga attgtggata tgaagcggtg gtatctgtgt ccgcaggtac
      781 gggtcgcgga tatcgtccag ctgaacggga atatccggcc acgatcacgc cagtggtggc
      841 agttattcag gatggtgtct cagtggcatg ttgatgtggt tatcgttgag ctgcgttcgt
      901 tcagtattgt tgcagcagta gagctggatg atgccagcca tttacgaccg gaacgcagac
      961 gccgggatat tcttctggaa gaggttctga ggcaggctgg tattccgttg ctcagaagcc
     1021 acgatgccag aaaactgctg cagatgaccg gagaatggct gaatacaaca ggggctgatc
     1081 agcagtcacc ggaacatcgt agctgacgcc ttcgcgttgc tcagttgtcc aaccccggaa
     1141 acgggaaaaa gcaagttttc ctcgctcccg gcgtttccat aactgaaaac catactattt
     1201 cacagtttaa atcacattaa acgacagtaa tccccgttga tttgtgcgcc aacacagatc
     1261 ttcgccacaa ttctcaagtc gctgatttca aaaaactgta gtatcctctg cgaaacgatc
     1321 cctgtttgag tattgaggag gcgagatgtc gcagacagaa aatgcagtga cttcctcatt
     1381 gagtcaaaag cggtttgtgc gcagaggtaa gcctatgact gactctgaga aacaaatggc
     1441 cgctgttgca agaaaacgtc ttacacacaa agagataaaa gtttttgtca aaaatcctct
     1501 gaaagatctc atggttgagt actgcgagag agaggggata acacaggctc agttcgttga
     1561 gaaaatcatc aaagatgaac tgcagagact ggatatacta aagtaaagac tttactttgt
     1621 ggcgtagcat gctagattac tgatcgttta aggaatttta tggctggcca cgccataagg
     1681 tggcagggaa ctggttctga tgtggatgta caggagccag aaaagcaaaa accccgataa
     1741 tcttctttaa ctttggcgag tcagaaagat taccggggcc cacttaaacc gtatagccaa
     1801 caatcaagct atgcggggag tatagttata tgcccggaaa agttcaagac ttctttctgt
     1861 gctcgctcct tctgcgcatt gtaagtgcag gatggtgtga ctgatcttca acaaacttat
     1921 taccgccagg taaagaaccc gaatccggtg ttcacacccc gtgaaggtgc ccggacgctt
     1981 ccgttctgcg ggaaactgat ggaaaaggcg gtgggcttca cctcccgttt tgatttcgcc
     2041 attcatgtgg cacatgcccg ttcgcttggt ctgcgtcggc gcatgccacc ggtactacgt
     2101 cgccgggcca ttgatgcgct gctgcagggg ctgtgttttc actatgaccc actggccaac
     2161 cgcgtccagt gttccatcac cacgctggcc attgagtgcg gactggcgac ggagtctgct
     2221 gccggaaaac tctccatcac ccgggccacc cgggccctga cgttccttgc agagctggga
     2281 ctgattacct accagacgga atatgacccg cttatcgggt gctacattcc gaccgatatc
     2341 acgttcacat ctgcactgtt tgctgccctc gatgtatcag aggaggcagt ggccgccgcg
     2401 cgccgcagtc gtgtggaatg ggaaaacaga cagcgcaaaa agcaggggct ggataccctg
     2461 ggtatggatg aactgatggc gaaagcctgg cgttttgttc gtgagcgttt ccgcagttac
     2521 cagacagagc ttaagtcccg tggaatgaag cgtgcccgtg cgcgtcgtga tgcggacagg
     2581 cagcgtcagg atattgtcac cctggtgaaa cggcaactga cgcgtgaaat ctcggaaggg
     2641 cgcttcactg ccagtcgtga ggcggtaaaa cgtgaagtgg agcgtcgtgt gaaggagcgc
     2701 atgattctgt cacgtaaccg taattacagc cggctggcca cagcttcccc ctgaaagtga
     2761 cctcctcaga ataatccggc acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc
     2821 acaaaaaatc agcgctacat acaaaaaaca acctcatcat ccaccttctg gtgcatccgg
     2881 tcccccctgt ttttgataca aaacacgcct cacagacggg gaattttgct tatccacatt
     2941 aaactgcaag ggacttcacc gtaaggttac aaccgttcac gtcataaagc gccagccgtc
     3001 aaccctacag ggtgcaatgt atcttttaaa cacctgtgta tatctccttt aaactactta
     3061 attacattca tttaaaaaga aaacctgttc actgcctgtc ctgtggacag acagatatgc
     3121 acctcccacc gcaagcggcg ggccccaacc gaagccggtt tagttacaac atgcaaacat
     3181 aaacccctaa aaaacccaag tccaacgcag aactgaaacc acaacgcccc tcctcataac
     3241 tgaaaagcgc cgccgccccg cccgaagggc gggaacagag tcgcttttaa ttatgaatgt
     3301 tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgagta cacgctcgta
     3361 agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa aaccctcgtc
     3421 gggaccactc cgaccgcgca cagaagctct atcatgactg aaaacgggta tggcttagca
     3481 gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc gggcttcggc
     3541 ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg ctggcgcggc
     3601 atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat attaagtggt
     3661 cgtttcaggg atatcgttaa gacttga
//
LOCUS       DADLTF010000250         3651 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.255, whole genome shotgun sequence.
ACCESSION   DADLTF010000250 DADLTF010000000
VERSION     DADLTF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.255"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(2..298)
                     /locus_tag="JN749_004083"
     CDS             complement(2..298)
                     /locus_tag="JN749_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HAY7370273.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            1050..1598
                     /locus_tag="JN749_004084"
     CDS             1050..1598
                     /locus_tag="JN749_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7370274.1"
                     /translation="MAVIGYCRVSTQDQTVDNQKLQIEKVHKVDKWFIDEAVSGATKA
                     TSRDGFRNMMNYVRCDDTLVVVAIDRLGRNTVDVLSNVEALQTKGVRVVSLREGFDLL
                     TPIGRAMLTMMAGLADLEKDLISERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQG
                     KTRLQIQEELGISRATYYRLAK"
BASE COUNT         1131 a          690 c          815 g         1015 t
ORIGIN      
        1 ctcagaacac tttcttaaat ggtttcactg aaacgtgttc atagactcct gccgctacgt
       61 acgggtcagc atcggcccag gcctgagctg cttccagcga ttcaaattca gcaataacgg
      121 ttgagccagt aaatcccgca gcccctggat cgttactgtc taccgctggc attggaccag
      181 ctgtcaacaa acgaccttca tcatgcagta actgtaaacg tgctaaatgt gccggacgaa
      241 cggaaaggcg tttttcgagg ctatcagctt tgtcttgagc gtaaataaca tacaacacgg
      301 gcacaactcc ttgttcggga aagttgtaaa gtacgttatt tgaaagggcg aagatctgca
      361 acggaaagat gatgtctttg ttaaggtcat gcataaagta agggtaatta cgccaaaaat
      421 gacattttca ctgatcctga tcgtcttgcc ttattgaata tgattgctat ttgcatttaa
      481 aatcgaggcc tggtttttct actgaaatga ttatgacttc aataaccctt gaatgaagtc
      541 tattcggttt tctaggagac ctgttaaaaa caggacaata tcaggtgaat gactatgcaa
      601 tttaatagta gatttccaag tatctaagca gttttacaca aattctatgc agtatcggta
      661 tagtctcttt ttagcataac ttattgaaat atatagagaa tctgtgaagt ctcttttcag
      721 ggaactatgc agggtgttag tcgttcctct ctcgactcct agaaaagtgc tatatacagg
      781 actatataac gatatataga tgtttggtca catggtgtaa ctatcaagaa acttaacgat
      841 ttcctacgtg aaagttagaa agtgatggtt atagttaccc tcctaaaatc ttcatagctc
      901 cttcataacc agtcttaaaa ggacttcctt ttgagacact ttcaacgtaa cggtagaatg
      961 caccctcaag tagtctcaaa aggttgtttt tggattatga gacagctaaa cttatgagac
     1021 taacttttga gacttctaag gggaacatta tggcggtgat tggttattgc agggtatcaa
     1081 cacaagacca gactgtagac aaccagaaac tacagataga gaaggttcac aaggtcgata
     1141 aatggtttat tgatgaggct gtctcaggag ctacaaaggc caccagcaga gatggtttta
     1201 gaaacatgat gaactatgta aggtgtgacg atacgttagt tgtggtagct attgaccgtt
     1261 tagggcgtaa taccgttgat gtgctctcta acgtggaggc actacagact aaaggtgtga
     1321 gggttgtttc tctacgtgaa ggttttgact tattaacacc aatcggtaga gcaatgctta
     1381 ctatgatggc tggtttagct gaccttgaaa aagaccttat cagtgaacgt agaaaggctg
     1441 gtattgaacg tgctaaagct gaaggggtac atatggggag gcctgtaaaa gcttcttcag
     1501 aggctgtaca gacgcttatt tcacaaggta agacgaggtt gcagattcag gaagaattag
     1561 gaatctctag agcaacttat tacagacttg ctaaataagt tactgaatag ttacatggat
     1621 gtagctgata tttggctttt ttgcaaaggg gatcacatag ctggtgtaac aggtacttag
     1681 cagctacaga catctgtcag atatttttga agattctcag agagggaatt tgaagagggg
     1741 ctactatgat tttataaaaa ttttcagagt ctgaaaagtt actgtacaga ttttatacaa
     1801 actaaaaagg actcccgtag gtgattcagt aatgaattac ttcacgaaga gtccctttac
     1861 aagtcgattt ttaatcgtgg ctacgacaat ccacccatac cagacctaac cgtaccccac
     1921 cttaccaagc cataccctac cccgccagac tataaggaag cgtagagaac cgatgaaggt
     1981 gtctgcacag ttgcatctta aacaaccatc acagtgatgt caaatagttc ttttaagtag
     2041 gaggggttcc cctatggtca catatcccct ctctcccgct tagtgtccct tgatagtatc
     2101 tagagagtat gcctgtaatg ttagagagta tgcctgtaat gtttctgtat caggtcagct
     2161 ataagaggtc tggatattac cttgtaagat tcttggtagt gattatgcag agggcttgat
     2221 aagagtgttt aacgggagga agagaaccat gaaaccgaag gtaggttcta tcttaacagt
     2281 gactgtaaga gatacctgaa aaaatacttt aacagaacta ccaagaagag agggaacgga
     2341 agagagggaa cgataagacc agaggtagtg acctgttaaa caggctgttt caaagtctct
     2401 taaaagtctg ttaagagggc taggtgaaca atcctgccat tttgtcaagt gttttgtctt
     2461 tagcaaaaag tgctaaactt aacaaaaagc actaaatgct aaagaggcga ttaagaaata
     2521 tactaagagg ggcgcttcgc ttggggtcac tatgttcttt ctctcccgtt aagaggattt
     2581 acagggacat ttcaaagccc tgataaggta agcccgatga taacagaaac agagggcaaa
     2641 accacttatc aacactgtga acgaataacg taaagggctg gttaagaaag tcggtttagg
     2701 gattggtaac aataccaatt agaagggagc ttccctttct ggggtgtcgc tctatgggtg
     2761 aagttaacct ttgaagttct ctgtgaagaa actgaacagc tattatattg gctaattaca
     2821 accctttgaa agataagcat ttttagaagt tactgttaag gttgcagagt tgtctgtgaa
     2881 gagacttgtc agttctctat attgggtggt tacaactcat tgaaaaataa gagcttttag
     2941 aggttactgt cagagaggtg aatacttgat agatacagtc ttccggttct gtcaggctat
     3001 tttttaacag atagcacaaa atgctaaaga tgcccctatg ccatctcgta caggggggtt
     3061 ttgaagggag tttaacgggg gtctgtgtgg tcattatcct taaggtcaag cgtccatgtg
     3121 cgaccgaaca gtttaaagac aacaagcact ctgacgagga cgtaacgaag tagcagatct
     3181 gcaataagtc caatacctcc accagcccat acatcaggtg accccagctc aggagggaga
     3241 tttaccatta gttttccttg taaggcgctc tcaatcgcat tctgacacaa aaaagtacct
     3301 gaccttatga gttgacctga agaggtcatt taactcgtta cagagcgatt gaagaggtct
     3361 gtgtgcaatg caataaccat gtgaccagat acatagccac gcctcatatt ttactctccc
     3421 cataaaccca ttacaccgca gatgttacga aattgttaaa aatacttggt attagtcttt
     3481 gaaaatgcta gcattgtata tataagttaa cagaataata acatgttatg aggggcagaa
     3541 tatgaatgag agacgcagaa tttagagcga tgcttcaggc atcaagagaa cgcaacaagc
     3601 acaactccta cgcttacaca aacaacccca ccagctacga agtcccaacc t
//
LOCUS       DADLTF010000251         3592 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.256, whole genome shotgun sequence.
ACCESSION   DADLTF010000251 DADLTF010000000
VERSION     DADLTF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.256"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            339..536
                     /locus_tag="JN749_004085"
     CDS             339..536
                     /locus_tag="JN749_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAY7370275.1"
                     /translation="MNAHDIEPTILRATGNTHSLDVTFIHEEDLATPWKPEQSSVYAH
                     VNPYGIFELDMETRLPIEVVA"
     gene            complement(618..2327)
                     /locus_tag="JN749_004086"
     CDS             complement(618..2327)
                     /locus_tag="JN749_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HAY7370276.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2759..3478)
                     /locus_tag="JN749_004087"
     CDS             complement(2759..3478)
                     /locus_tag="JN749_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HAY7370277.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1067 a          721 c          641 g         1163 t
ORIGIN      
        1 tgatacaact ggtctggtat atacagtttc tttgagtgcg gttacgtcca cagaggtaac
       61 ggtaaaagta accccttgtc gggaccactc cgaccgcgca cagaggcatt ttcatcgctg
      121 acaggccgca ttgcctcgca gggctggagg aggatcggtg atatccggac gaccgtaccg
      181 gcttacgccg ggcttcggcg gttttactcc tctgccatgt gcaacaacgg agtgccgcct
      241 tacatgccgc tggcgcggca tatcctggta acatttttgt tgataccacc ttccggcctt
      301 atcctgacca gtgggctttc ctggcatcga tgataccgat gaacgcgcat gatattgagc
      361 cgacgatatt acgggcaact ggaaatactc actctctgga tgtgaccttt atccatgaag
      421 aagatttggc cacaccatgg aaacctgaac aatcatcagt ttacgcccat gtaaacccct
      481 acgggatatt tgaactcgat atggaaacaa ggttgccaat cgaggtggtt gcctgaaagt
      541 tggcagttac ccggtcacta caccttgttg gagtgaacat atcaaaaaca ggtaattaca
      601 caatttaaat tacagtctca taaaaaatga ttaaatctat ttattatttc aataaacgtt
      661 tttttcatct tctttgaaaa ttgttctgct atagtatccg gttccagatt gttctctttt
      721 attattctac aatactctaa tattgtttct ttgtctctat ttactacaga tacaaataca
      781 ccaggaatac catctttatt agttgcggac agcatttcga ccaatcgtct tgtggtaaaa
      841 ctatagtttt ttagaatatt acaaaaggca cgtattgttt cggtatggcc attataaaat
      901 gccataaaaa gtcctgatgt accgttggga ttttttatcc ataataattc atctatatat
      961 tcctggtgag gggggagtat ttttagtata tctccatacg ccataatggc ctttgtgtgc
     1021 ccattttgaa gtgccataaa caacccatgc tttactttca ataattcttt aatttgttca
     1081 ctactaagca ttttcttatt aagcagactt ccgaacatac gaatactatc tgcatgtcca
     1141 ttctgcaatg caaaaaataa cccaggtaaa tcatggctgt ctttcgcagc taatagatat
     1201 aatctcttat cggaagttaa cggggtttta aagactattt ccccataggc ttttattgtg
     1261 tctacatgtc cgttcataaa agacatgcat aaacccggca cattgtttgc ccctaccgca
     1321 gcaagcaatg taagtattgt ttcttgacta agattgcaca tatttaatat cttaccatat
     1381 acttgaataa tgtctgaata tccgttttga agtgctatat aaaatgcagg agtaccatca
     1441 gagcttctcg tggccagaag gttcaccagt ttagattgag aaatttcacc atttttgtat
     1501 ttttccatca gtataaataa tacttctgtc agattctttg ttatcgcatg atatataact
     1561 ctggaattga tcaattcatc tggtaaaagt ttcactgtgt ttgttagagg ggaattatgc
     1621 ctatcttcaa atattacctg cccgggttgc tccagaccat aaattttcca ataaaaatca
     1681 tcaaaaaaat ccttagcgga taattgactc agttcgaaat ggctatcaca agtaaataca
     1741 gtacgtttat gagtaacagt tcgattagga tcgaaaaact gaacaaccca tttttcactt
     1801 tcttgtacgc gctttatttt caaccctaac gccatttggt ggtttaatgt gcatgctgat
     1861 aatgttctga caaagtctcc attttttttc atgttataaa aaaaattagt aataactgtt
     1921 ccccactttt tattttccac aaaataaata taagctggtg attttgaaaa atcttccgtt
     1981 ttttccaaac atacattttt tgcaatagca tcaggggtgc taagaagctg atagtttacc
     2041 ttcccatttg tttccataaa attcatttcc caataggaag ctaggtgacg gcaaactatt
     2101 tgtcttccat atatatcttt tgccactcca tttatattta ccttcacatt tctcttttct
     2161 gaaaaataag gtgctgtttt attcttaaac tgaattagtt cactggagtt tgcgaccctc
     2221 ataatagaac ttgttattgt tctattatct ctgttaacag acatatctgt tgataacgaa
     2281 ttttttgttg aaaatatact agatgaaaat gttttattta acggcataga agacaaccat
     2341 agagatgtta taatactatt agattgcagt tactaatagt aaaagcctcc ttacaaggag
     2401 gtagcttatt cagaatgctc ctgtaagata gcgagctcgt caacgataca gatgatctga
     2461 ttatattgtg caaaaggaga acaggaaaat aggcactgga aaagatgaag gcctgatggg
     2521 agcattaact aatgcaaaat ccgacaggtt aatatcccga aaagcaatca aacgactcaa
     2581 acaggagatg cacgctgaag tggtggacaa ctgttcggat ttcagatgag tagctcagca
     2641 gtttatgagc cgaatgctct gtcacgggca ggaatcaggc tgcatacgca gtgccaaatc
     2701 taatcgtagt tattctaacc agttttcaag ctacaaggtg gtgtagctgg catcttctct
     2761 actctatcat caaacgataa aatggttcct ctcgtaacat ttgctcttgc ctttcacttc
     2821 catctcgatc gcttcgtagt tcattacgat agctgacata tttccagtct tcaggtctaa
     2881 catctgacgc cggatactcc ccgggagcga ttttgcttct taatagatta gactcgagac
     2941 acattataaa ttgtcgtatt ttatgaagta ataaagcact atattgcgag cattcctgat
     3001 ctgattttac atatagcgta aactgagctc ctatccccac acgagattgc tgagaaactc
     3061 gattcatatc agttatcttc catttatcta taggactgtc ctctgaaaat aataaaccag
     3121 acaaaatttg gaatgccaga ggaacttgct ccctagctat actaatatga aacttgtccc
     3181 caacaaaatc ccccttactc tgaggatttt cacgacatgc atgaataaac acatctccac
     3241 tatggttcac aatctggaat ccttggtaca tgacagaaaa aaattgacat atatcagagt
     3301 aagttggcac gttcgatgct actttgaaat agtacgctgg gtattgccgt atttggttgt
     3361 acaatatatt gaagttattt ttcaatcttt cattcgcaga aagcatctgc ggtatttcag
     3421 acctcacaac attcaaagaa tctaaattta gttttagaca gggctttttt atgggcatag
     3481 aaaacgtcct ctataaaata gatatattat cttatattga ttgtttatca tcttttataa
     3541 aacgatacat tatatgtaat gtatgtaagc gtaagggtct tctccgatca tg
//
LOCUS       DADLTF010000252         3517 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.257, whole genome shotgun sequence.
ACCESSION   DADLTF010000252 DADLTF010000000
VERSION     DADLTF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.257"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JN749_004088"
     CDS             complement(63..1505)
                     /gene="cusS"
                     /locus_tag="JN749_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HAY7370278.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPD"
     gene            complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JN749_004089"
     CDS             complement(1495..2178)
                     /gene="cusR"
                     /locus_tag="JN749_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HAY7370279.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2335..>3517
                     /gene="cusC"
                     /locus_tag="JN749_004090"
     CDS             2335..>3517
                     /gene="cusC"
                     /locus_tag="JN749_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HAY7370280.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKMEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQ"
BASE COUNT          862 a          911 c          921 g          823 t
ORIGIN      
        1 ggtatgacta ttgcggcacg ttatttttac actggttata aaagttgccg tttgctgaat
       61 aattaatcgg gtaatgtgat aacaaacctt gtcccccgcg catctgacgt taccgcaacc
      121 gtgcctttat gcgcgacaac aatcgatttc actatcgcca gcccaatacc gctaccttca
      181 ccttttcgct ggcgggaggg gtcaacgcga tagaaacggt caaacaatcg cggtaagtgc
      241 tcgggcgcaa tgggcgtacc ggggttttcg acggaaactt gcacctggtg atcgaccgtc
      301 tgacagcgca ctacaattgt ctctctggtt ggcgtataac gcagggcatt agaaagcagg
      361 ttgcttagcg cccgacgcag catcaacgga tcgcccgcaa cctgacgttt gtcgccaaca
      421 aaccgcaact ccacgccgcg atcttccgct agcgcctcga aaaaatcgaa cactttgccg
      481 acttcatccg ccaggttgag cattttcttt tcggggatta gctggttgtt atcggcctga
      541 gcgagaaaca gcatatcgct gaccattttc gccattcgcg tcagctcttc gagattagag
      601 tagagcacat cttccagctc cttctggctg cgcgactggc tgagggcgat ttccgtttgc
      661 gtaatgagat tagtaattgg tgtgcgaatt tcgtgggcga tatccgctga gaaattggac
      721 tggcgagtaa agacatcctc aatacgctcg atcatatggt tgaacgacag taccagctgt
      781 tccagctcaa tcggcacggt ctgcgggtca aggcgaacgt cgagatcttt cgaggtgata
      841 ttctggattt gacggctgac gctgcggatc ggcgcgtgac ctttatgtac cgccaacagc
      901 acgataaaga cgatcaggat gctgattacc gatgcggtca taataagttt attcatcaaa
      961 tcatttatgt aatgaagatg aaaatcgatc gacagcgcga tgtagagcgt ataaatcggt
     1021 ttgccgtcca ccaacgggcc aaccggcaag ttaatcatcc gccagttgct gtgttccata
     1081 tgcccgtgac cgtggcctgg catcataatc gtcgggccgg aaaggagata cacctcgcca
     1141 ccccgcgcgt ctttatcggg tatggcatca cgtgcaaact cgcggatatc aggcgcaccg
     1201 ggggagtgat acaccgtttt gccgtggcta tctgccaggg aaatcaacac gttggaataa
     1261 ccactgacga tatcttccag cgtcattaag cgtcgggctt gcgtttcgtc agggtgattt
     1321 agtacccgtt caagggtggc gctaatctct tttaaatcat taatgtcctg ctcggcaaaa
     1381 tgcactttta ctgagtggat catgatccat gcaaaggcga aaaacgccgc gatggtggcc
     1441 aggctgataa aaaaggtcag gcgggttgcc agcgaaaacg ggcgctgaaa tggcttactg
     1501 accatccggc acctcaagca tgtaacccac gccgcgcacg gtttgaatca gcttcggctc
     1561 aaagtcgttg tcgattttgc cgcgtagccg cttcaccgcc acatcaatgg cgttagtgtc
     1621 gctgtcgaaa ttcatgtccc atacctgcga ggcgataagc gagcggggca gcacttcgcc
     1681 ctgatggcgg agaaaaaact ccagcagagt aaattcttta ctggtcagag tgatgcgcgt
     1741 gccgctgcgg gtgactttgc ggctgacgag atcgaccatc agatcggcaa cctgaaactg
     1801 actttcgata atcaccgccg ccccgcgccg caggagagtg cgcacccgcg ccagcagttc
     1861 agcaaaagcg aacggtttca ccaggtagtc atctgccccc aactccagcc ccttgacgcg
     1921 atgttcaatg gtgccaagcg cggtaagcaa cagaatcggc atccctttat tggcggagcg
     1981 caacatgcgc acgatatccc agccgttcac gtctggcagc ataatatcga ggattatcag
     2041 gtcataatca ccggtcatcg ccagatggta gccattcagc ccgttgtcgg ccaaatcgac
     2101 cacaaaaccg gcttcggtta accctttggt caagtattct ccggttttct tttcatcttc
     2161 gacaatcaac agtttcatat ttcctccgca tgttgcccgg gcaattctag agtagcggga
     2221 tcagatggca atcgcttatt ggcaaaatga caattttgtc attttcctgt caccggaaaa
     2281 tcagagcctg gcgagtaaag ttggcgacat aaaatcacca gaaattatga gcctatgtct
     2341 ccttgtaaac ttctgccatt ttgtgtggcc cttgcgctaa ctggttgttc actggcaccg
     2401 gattatcagc gtccggtaat gcccgtaccg cagcagttct cactcagcca gaacggcctg
     2461 gttaacgcag cggataacta ccagaacgcg ggctggcgca ccttttttgt tgataatcag
     2521 gtgaagacgc tgattagcga ggcgctggtg aataaccggg atttgcgcat ggcgacgctg
     2581 aaagtgcagg aggcacgggc gcaatatcgt ctgaccgatg ccgaccgcta cccacagctc
     2641 aatggcgagg gcagcggcag ctggagcggc aatcttaaag gcgattcagc cacgacgcgg
     2701 gagttctcga ctggccttaa cgccagcttt gatctcgatt ttttcggtcg cttaaagaac
     2761 atgagcgaag ccgagcgcca aaattattta gccactgagg aagcccagcg cgcggtgcat
     2821 attttgctgg tttctaatgt cgcgcaaagc tatttcaatc agcagctggc gtatgcgcaa
     2881 ttgcaaatag ccgaagaaac gctgcgtaat tatcagcagt catatgcgtt tgtcgaaaaa
     2941 caactgttga ccggtagcag caatgttctg gcgctggaac aggctcgcgg ggtgatagaa
     3001 agtacccgca gtgacatcgc taaacgtcag ggggaactgg cgcaggcgaa taatgcattg
     3061 caactgttat tgggaagcta cggcaagcta ccgcaagcgc agacagtaaa cagcggcagc
     3121 ctgcaaagcg ttaaattacc ggcgggcttg tcgtcgcaaa tcttattgca gcgcccggat
     3181 attatggaag ctgaacacgc gttaatggcg gctaatgcta atattggtgc tgcgcgtgcg
     3241 gcatttttcc cgtctatcag cctgaccagc ggaatatcaa ccgccagcag cgatctgtcg
     3301 tcattattta acgctagcag cgggatgtgg aattttattc ctaaaatgga gatccccatt
     3361 tttaatgccg gacgcaacca ggccaatctg gatatcgccg aaattcgcca gcagcagtcg
     3421 gtggtgaatt atgaacagaa aatccagaac gcttttaaag aggtggcaga tgcgctggca
     3481 ttacgtcaaa gcctggacga tcaaatcagc gcccagc
//
LOCUS       DADLTF010000253         3514 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.258, whole genome shotgun sequence.
ACCESSION   DADLTF010000253 DADLTF010000000
VERSION     DADLTF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.258"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            289..807
                     /locus_tag="JN749_004091"
     CDS             289..807
                     /locus_tag="JN749_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HAY7370281.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            814..1047
                     /locus_tag="JN749_004092"
     CDS             814..1047
                     /locus_tag="JN749_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MbeD"
                     /protein_id="HAY7370282.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(1349..1816)
                     /locus_tag="JN749_004093"
     CDS             complement(1349..1816)
                     /locus_tag="JN749_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370283.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(2144..2485)
                     /locus_tag="JN749_004094"
     CDS             complement(2144..2485)
                     /locus_tag="JN749_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370284.1"
                     /translation="MRRWRFSIGSAPLTSITKIDAQVRGGETRQDYKDTRRFPLEAPS
                     CALLFRPCRLPDTCPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAP
                     YPVTIVLSPTR"
     gene            complement(2886..3077)
                     /locus_tag="JN749_004095"
     CDS             complement(2886..3077)
                     /locus_tag="JN749_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7370285.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            3142..3447
                     /locus_tag="JN749_004096"
                     /pseudo
     CDS             3142..3447
                     /locus_tag="JN749_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000955993.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
BASE COUNT          844 a          787 c         1042 g          841 t
ORIGIN      
        1 cctggcagac gctggttaat caccattacg ggctgcatga cccgaacgcc ccggagaacc
       61 gcagggcgct gacactccct gataatctgc cggaaacgaa acaggcgctt gcagagagcg
      121 tcacacgcgg gatagacgct ctttatcatg tcggggagat aaaaggccgt caggatgtga
      181 ttcaggcgct cacagaggca gggcttgaag tggtcagggt gacgcgaagc agtatcagca
      241 ttgcagaccc gaacggtggg aagaatatca ggctgaaagg agcattctat gagcaatctt
      301 ttacagacgg gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa
      361 atgctgaacg acgagttcag gaagctcgaa gaatctgtaa gcaaggctgt gacatcaaac
      421 gagacgaaaa tcaaagacgc tatagccctg ttcacagctt cgacagagga atcactgaaa
      481 aaacaccggg aaggggtgaa agaggcgatg atgcagcaca ggaaggacgt gttaaagctg
      541 gcagggaata cgggcatgat gttactgggg atggtcattt tcctgtttac cgtgagtggc
      601 gggacgctct ggtatcttgg cgggatgata caggcgaacc tggaagaaat caggaagcag
      661 aacgagagtc tggagaagct gaacgcgaag acatggggcg tggagtttgt gcaggacggg
      721 aacaggaaat ttctggtcct tccttacggg aagtcagcag aggtgatttc ctttcagggg
      781 aaggaatggg tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg
      841 aacgccttag agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc
      901 ttcgtggaat tgcagaaaat gttttcgctt acgcaacggg acaacgcgat gctgaacgag
      961 cgggtcatgc agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc
     1021 cagttataca gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg
     1081 cgttatccct ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggtcctt
     1141 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaggagg ggttaatgtg
     1201 gcctgtacca tctggttgca taggtattca tgcggttaaa atttatcagg cgcgatcgcg
     1261 gcagtttttc gggtggtttg ttgccatttt tatctgtctg ctgccgtgat cgcaccaaac
     1321 gcgtttcagt ggtgcgggca ataagatatt acgttaaatt gtgagagaat ggtccccata
     1381 tactgacttt atcatacgga tagggttggt agtgggtcga ttgggtttcg gcattgtttc
     1441 cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat tctcctgagc
     1501 ctggaaagtc taagtgttta gaattgatgg caaaatatct tccgtccgat tcttgaagta
     1561 gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt tgtagttgtt
     1621 gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt attattctct
     1681 cttttaataa gctgtttacc acaggattta gttgttgtct aaagcacgca gataatgcca
     1741 ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg
     1801 atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg gctataaaac
     1861 aaaattttca tagagtgatt caagatcttc ttgagatctt ttttggcggt gcgtaatctg
     1921 ctgcctgcaa acaaaaaaac caccgttatc aacggtggtt tgtttgccgg aatctagagc
     1981 taccaactct ttcttccgaa ggtaactggc ttggtggagc acagatacca aatgctgtcc
     2041 ttctagtgta gccgcagtta ggccaccact tcaagaactc ttaatatctc aatccacctt
     2101 atccagttac caatggatgc tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     2161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa
     2281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2461 ctatggaaaa acgccagcgg cgcagccttt ctttgccctt tgcctttttg ctcacatgtt
     2521 ctttccggcc ttatcccctg attctgtgga taaccgtatt accgtctttg agtgagctga
     2581 taccgctcgc cgcagccgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga
     2641 gcgccggaag ctgcattttc tccttacgca tctgtgcggc atttcacatc ggctatggtg
     2701 cacttttcat accatccgca ctgatgccgc ataagtaagc ctgtgtacac tccgctatcg
     2761 ctacgtgact gggtcatggc tgcgccccga cacccgctaa aacccgctga cgcgccctga
     2821 cgggcttgtc tgctcctgac atccgctcac aggcaagctg tgaccgtctc cgggagctgc
     2881 ttgtgtcaga ggttttcacc gtcatccccg aagcgtgcga ggcagctgcg gtaaagctcg
     2941 tcggcgtggt cgtggagcga ttcacagata tcagcctgtt catccgcgtc cagttcgttg
     3001 agcttctcca gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt
     3061 ttttcctgtt tagtcactga tgcctccgtg taagggggat ttctgtttta tggggtaatg
     3121 ataccgatga atcgcgagag gatgctcacc atacgggtta ctgatgatga acatgcccgg
     3181 ttactggaac gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt
     3241 gagccggttg cacgctccgg gaagctgccg acactggcac cgccgttact gcgtcagctg
     3301 gccgccattg ggaataacct gaatcagaca gcgaggaagg tgaacagcgg gcagtggtct
     3361 tccggtaacc gggttcaggt ggtggccgca ctgatggcca tcggggacga gctgcgccgg
     3421 ctgcgtctgg ccgtcaggga acaggggcgc gggatgatag ttaagtttca tgcccgggga
     3481 aaaggtggtg gcagtggtcc ggttgattac ctgc
//
LOCUS       DADLTF010000254         3501 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.259, whole genome shotgun sequence.
ACCESSION   DADLTF010000254 DADLTF010000000
VERSION     DADLTF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.259"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            51..467
                     /gene="torD"
                     /locus_tag="JN749_004097"
                     /pseudo
     CDS             51..467
                     /gene="torD"
                     /locus_tag="JN749_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
     gene            complement(560..865)
                     /gene="cbpM"
                     /locus_tag="JN749_004098"
     CDS             complement(560..865)
                     /gene="cbpM"
                     /locus_tag="JN749_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HAY7370286.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(865..1785)
                     /gene="cbpA"
                     /locus_tag="JN749_004099"
     CDS             complement(865..1785)
                     /gene="cbpA"
                     /locus_tag="JN749_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HAY7370287.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            2046..3302
                     /locus_tag="JN749_004100"
     CDS             2046..3302
                     /locus_tag="JN749_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HAY7370288.1"
                     /translation="MGSNIHGMSCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
BASE COUNT          975 a          729 c          807 g          990 t
ORIGIN      
        1 accttaaaat caccatcata cactaaatca gtaagttggc agcattacct gccacgctga
       61 cagtacgtga cgatgcccgt ctggaactgg ccgctgactt ttgcggcctg tttctgatga
      121 ccgacaaaca agcggcgctg ccgtatgcat cggcctacaa acaggacgag caagagatta
      181 aacgcttgtt agttgaggca gggatggaaa ccagtggcaa tttcaacgaa ccggcagatc
      241 atctggcgat ctatctcgag ttgttgagtt atctgcattt ttcgctggga gaggggaccg
      301 ttcctgcgcg aagaatcgac agtttgcggc aaaaaatact gacggcgcta cggcaatggt
      361 taccagagtt tgctgcgcgt tgtcgacagt atgacagctt tggtttttac gcggcactaa
      421 gccagttatt gctggtgtta gtggagtgcg accaccaaaa cagataacgt cgtttatgcg
      481 cctgaaaaga cgcgtttagc gtcgcatcag gcattatggc gcagttgccg gatgcgtcat
      541 cacatccggc aaacacacat cacggatgag ctacaaaccg ggaaagccgc tggcgtagca
      601 ggcggttttc ctgcttcagg tgcgcaatat catccattaa cgtcagcgcc accgcgatcc
      661 ccggccagtc cagagccagt tcatgacgca ggcgtaccgc gcgttgcacc acaatggcgg
      721 catggtcgtc aaatacccag gttgtttcct ggatctcacg cggttcaacc acccccaaac
      781 cgacaatttc attcaactcc tcttcagaga tgccggtatg caggcaaaat tcggtaatag
      841 taaaagtcac cgtaacatta gccattatgc tttcccccaa tctttacgtg gatcaaaaga
      901 cgactgggcg tctgccagtt gctgccacag cgcggcagtg ttttcatccg gtttcggcgg
      961 catcacgatt ttcagtaccg catacagatc gccggtctgt tttttgctca ccagaccttt
     1021 gcctttaacg cgcaatcgtt gcccggcctg gctgcctggc gggatagtca gcaaaatgct
     1081 ttctttcagt gttggaacgg tgactttagt acccagcgcc gcttcccacg ggctaaccgg
     1141 caccacaatt tccagatcct ggccgacaat atcgaacagc ggatgtggcg caatatgaat
     1201 caccagccac aaatcgccat ttggaccgcc gttttcgccc ggcgtcccct ggcctttcag
     1261 gcggatgcgt tgaccattgc cgacgcctgc cgggatcttc acattcagcg ttttcgggat
     1321 ttcctgttcg atcatgccaa aggcgttata aaccggcagg ttatagctga tggtacgctt
     1381 atgctcagta agcgtttctt cgaagaatac cgccacttcg atttcaatat cgtggccgcg
     1441 tgcggcgggg cgttgacggc tctggcgggc atgctgaccg aaaattgacg agaagatatc
     1501 gtcaaaatct tcggcattaa aactctgacc gtcgccatga tggaactgac ggttaaattg
     1561 cggatcgttg cgatgttgcc acatctgatc atactcagcg cgacgttgtt catcacttaa
     1621 cacttcccag gcttcagcga cctctttgaa gcgggcttcg gcatccggtt ctttgctgac
     1681 atcaggatgg tatttacggg ccagtcgacg ataggcggtc ttgattgtct tgagatcgtc
     1741 cgtcggtttc acgcccatga tggcgtaata atcctttaat tccatagcgt tatctcgcgt
     1801 aaatcaacac aaattgaagg aacccctgta aggtaactcc tataagtgta gggtaatcct
     1861 caaaatttca tatgccaaca cagaatatgt tattgaaatc atcgcggaga ggaggtcgcc
     1921 atcaagatgg gttgctgaac atattttaaa caggtgaaaa agggtgagcg atttttgata
     1981 gttgaaccag gcactttaag tttaactagg gcgtcattat ttattaaatt ttatagacgc
     2041 tatatatggg tagtaatata catggaatga gttgcactgc aaataattat ttgaaacagg
     2101 cctggaacga tataaaaaat gagtacgaaa aaaatcaaac atattcaatc acgctttttg
     2161 aaaacacact ggtgtgtttt atgcggttgt acaatgaact cagacgtaaa gtaaatgaag
     2221 aggatactcc atgtctggaa tgtgaatcac tagaaaaaga atttgaggaa atgcagaatg
     2281 ataatgatct atcattattt atgagaacat tgcgtactaa tgatacacaa atttattcag
     2341 gggtttcagg aggtattaca tatactatac aatatgttca agatgttgat attgttagag
     2401 tgtccttgcc gggcagaggt tcagagtcta tcacagattt taaaggttat tattggtatg
     2461 gctttatgga atatatcgaa aacattaatg cgtgtgatga tgttttttct gagtattgtt
     2521 tagatgatga aaatatgagt atccagccag aacagataaa tatgccgaga atatctgatt
     2581 tagatactgg tattgatttg tctggcatat cttttattca gagtgaaatt aataaaacat
     2641 atggattaaa atacgctcca gtagatggcg atggatattg tctgttaaga gccatactgg
     2701 ttttaaaaga acatgagtat tcatgggcgc tgggcagcca taagacgcaa aagcaagtat
     2761 acgaagagtt tattaaaatt gttgataaac aaacgatcga ggctcttgtt gatacggcat
     2821 tctataatct cagggaagat gtaaagacat tatttggcgt tgatctgcaa tctgacaaca
     2881 aaattcaggg tgtgggtagt tttatgtcat ggagctttct gttttttaaa aaacaattca
     2941 ttgatagttg cttgaatgac aaaaaatgca tactgcattt acccgagttt atatttaatg
     3001 ataacaagaa tttgcttgct ttagataccg acacgtcgga taggattaaa gcggtgaaga
     3061 attttcttgc tgttctttca gatagcattt gctcattatt tattgttaat agtaatgtgg
     3121 catcaatctc cttgggaaat gaatcctttt caacagatga agatcttgag tatggttatt
     3181 taataaacac tggcaatcat tatgatgttt acctccctcc tgaacttttt tctcaggctt
     3241 ataagttaaa aaataaggaa atgaatgcgc aacttgacta tttaaatcgt tatgcaactt
     3301 aatggcaaag gcatatgcta aaaaccattg ttattagtct cacacttttt tattggtaaa
     3361 tattgtctct gtattggtaa cgccgcagat attctgttta gccacaggtg caattatcag
     3421 cggcgtacgc gaggcagggg ctaatcaggc atagtttgcg tcaaaccttg cctgtttttg
     3481 aagatgtata tagaaaaaca g
//
LOCUS       DADLTF010000255         3471 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.260, whole genome shotgun sequence.
ACCESSION   DADLTF010000255 DADLTF010000000
VERSION     DADLTF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.260"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..1988
                     /locus_tag="JN749_004101"
                     /pseudo
     CDS             1..1988
                     /locus_tag="JN749_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
     gene            1994..2707
                     /gene="ybiX"
                     /locus_tag="JN749_004102"
     CDS             1994..2707
                     /gene="ybiX"
                     /locus_tag="JN749_004102"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706682.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HAY7370289.1"
                     /translation="MWGAGPTFWRTCMMYHIPGVLSPQDVAHFREQLEQAEWVDGRVT
                     TGAQGAQVKNNQQVDTRSALYAALQNEVLNAVNQYALFFAAALPRTLSTPLFNRYQNN
                     ETYGFHVDGAVRSHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGD
                     LVLYPSSSLHCVTPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENE
                     EILSLLNLYHNLLREWSEI"
     gene            2781..3047
                     /gene="ybiI"
                     /locus_tag="JN749_004103"
     CDS             2781..3047
                     /gene="ybiI"
                     /locus_tag="JN749_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HAY7370290.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3312..>3471
                     /locus_tag="JN749_004104"
     CDS             3312..>3471
                     /locus_tag="JN749_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370291.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEA"
BASE COUNT          894 a          922 c          872 g          783 t
ORIGIN      
        1 ccggcgtggg tgcgtttttt gcgggtgaga acggtaactc caccactggc gacgccattt
       61 atatgcgtgg tgccgatacc tctaacagta tttatattga tggtattcgc gatatcggca
      121 gcgtctcgcg cgacaccttc aataccgagc aggtcgaagt gattaaaggg ccgtccggca
      181 ccgactacgg gcgcagcgca ccgacaggct cgatcaatat gatcagcaag cagccgcgca
      241 atgattccgg cattgacgcc tccgccagta ttggcagcgc ctggttccgc cgcggcacgc
      301 tggacgtcaa tcaggtcatt ggtgatacta ccgcggtgcg cctgaatgtg atgggcgaaa
      361 aaacccacga tgccggacgc gacaaagtca aaaatgagcg ttacggcgtc gccccttctg
      421 tctcttttgg ccttggtaca gcgaatcgtt tgtatcttaa ttatctgcat gtcactcagc
      481 acaacacgcc agacggcggc attccgacca tcggtttgcc gggctattct gccccatctg
      541 cgggagcggc ggccctgaat cattccggaa aagttgatac tcataacttt tacggcacgg
      601 attccgatta cgacgattcg accaccgaca ccgccaccat gcgttttgag cacgacatca
      661 acgataacac caccattcgc aatactaccc gttggtcgcg cgtaaagcag gattacctga
      721 tgacggcgat tatgggcggg gcgtcgaata ttactcagcc caccagcgat gtgaatagct
      781 ggacctagtc acgcacggcg aataccaaag atgtgagtaa taaaattctc accaaccaga
      841 ccaacctgac ctcgacgttc tataccggtt ctatcggtca tgatgtcagt accggcgtgg
      901 aatttacccg tgagacgcag actaactatg gcgttaatcc ggtgacgtta ccggcggtaa
      961 atatttatca tcctgacagc agaattcatc ccggcggcct gacgcgcaac ggcgcaaacg
     1021 ccaatggtca gacggatacc ttcgcaattt acgccttcga tacgctgcaa atcacccgtg
     1081 attttgagct gaacggcggg atccgtctgg ataattatca tactgaatat gacagtgcca
     1141 ccgcctgcgg cggcagcgga cgcggtgcca tcacctgccc agctggtgtg gcaaaaggtt
     1201 ctccagtcac caccgtcgac accgccaagt cgggcaatct ggtgaactgg aaagccgggg
     1261 cgctgtatca cctgacggaa aacggcaatg tctatattaa ctatgccgtt tcccagcagc
     1321 ctccgggcgg caacaacttc gcccttgcgc agtctggcag tggtaacagt gccaaccgca
     1381 ccgattttaa accgcaaaaa gccaacacca gcgagattgg caccaaatgg caggttctgg
     1441 ataaacgcct gttgctcacc gccgcgctgt tccgtactga tatcgaaaat gaagttgagc
     1501 aaaatgatga cgggacttac tcgcaatacg gtaagaaacg cgtcgaaggc tatgagatat
     1561 ccgtggcagg gaatatcact cccgcgtggc aggtgattgg cggctatacc cagaaaaaag
     1621 caaccatcaa aaacggcaaa gatgttgccc aggatggttc ctcatcgctg ccgtataccc
     1681 cggagcacgc cttcacctta tggagccaat atcaggcaac cgatgatatc tctgttggcg
     1741 cgggcgcacg ctatatcggc agtatacata aaggttcaga cggcgcggtg ggaacgccag
     1801 catttacaga aggttactgg gtcgcagatg ccaaactggg gtatcgggtt aatcgcaatc
     1861 tcgacttcca gctaaacgtt tacaacctgt ttgataccga ttacgtcgcc tcaatcaaca
     1921 agagcggcta ccgttatcac ccgggcgagc caagaacctt cttgctcaca gccaatatgc
     1981 atttctgatt cagatgtggg gcgcaggccc cactttttgg agaacttgta tgatgtacca
     2041 cattcccggc gtgttatcgc cacaggacgt cgcccatttt cgcgaacaac tggaacaagc
     2101 cgaatgggtg gatggacgcg tcaccaccgg agcacaaggt gcacaagtta agaacaatca
     2161 gcaagtagac acccgcagcg cgttatacgc cgcactgcaa aatgaggtgc tgaacgcggt
     2221 taaccaatat gcattgttct ttgccgctgc cttgccgcgt accctttcca cgccgctgtt
     2281 taatcgctat cagaacaatg aaacctatgg tttccatgtg gatggcgcag tacgcagcca
     2341 tccacaaaac ggctggatgc gcactgacct ttctgccacg ctgtttttaa gcgatccaca
     2401 aagctacgac ggcggcgaac tggtcgttaa tgacaccttt ggacaacatc gggtaaaact
     2461 cccggcaggt gatctggtgt tgtatccctc cagcagcctg cattgcgtga cacccgtaac
     2521 ccgcggcgta cgagtggcat catttatgtg gatccagtcg atgatccgcg atgataaaaa
     2581 gcgcgccatg ctgtttgaac tggacaaaaa tattcagtcg ctgaaaagcc gctatggtga
     2641 aaatgaagag atcctgtcgc tgctcaatct ttatcataat ctgctgcggg aatggtcgga
     2701 gatctgacgc ttgaactgcc caggttcgtc cctatattag ctacacttaa ctgtacaagc
     2761 attgatatgg ggaggacgat atggcatccg cttgggctaa cgatgacgcc gtcaacgaac
     2821 agatcaacag tacaattgaa gatgcgattg cccgcgctcg gggtgaaatt ccgcgtggcg
     2881 aaagcctgta tgaatgtgaa gagtgcggtg cccccatccc gcaagcccgt cgggaagcca
     2941 ttcctggcgt gcgcttatgt attcattgtc agcaggagaa agatttacaa aaaccagcgt
     3001 atacaggata taatcgcaga ggttcgaaag acagccagtt acgttaacta caactggcga
     3061 gatgcatagc gagtataatt tgtattttgc gtggcattcc gtgcctttaa cgctgctgtg
     3121 actgccacgc ggcataaaac aagtccataa gtacaacaaa taaatggttt atcagtatgt
     3181 tagatatcaa tcaatttatt tgaacaaggc ggtcaattct cttcgatttt atctctcgta
     3241 aaaaaacgtg atactcatca catcgacgaa acaacgtcac ttatacaaaa atcacctgcg
     3301 agagattaat tatgaaaact atcaatactg ttgttgctgc tatggctctt tcaactctgt
     3361 catttggcgt gttcgcggcg gaaccggtaa cggcatccca ggcacagaac atgaataaaa
     3421 tcggcgtggt ttctgccgat ggcgcatcca ccctcgatgc cctggaagcg a
//
LOCUS       DADLTF010000256         3380 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.261, whole genome shotgun sequence.
ACCESSION   DADLTF010000256 DADLTF010000000
VERSION     DADLTF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.261"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..934)
                     /locus_tag="JN749_004105"
     CDS             complement(<1..934)
                     /locus_tag="JN749_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HAY7370292.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFIVYSFACFFIGKFIANILM"
     gene            complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="JN749_004106"
     CDS             complement(962..1882)
                     /gene="rbsK"
                     /locus_tag="JN749_004106"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAY7370293.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGMQSSYPSIEQFNEFLTLNE"
     gene            2188..2970
                     /locus_tag="JN749_004107"
     CDS             2188..2970
                     /locus_tag="JN749_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAY7370294.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPALSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(2972..3070)
                     /locus_tag="JN749_004108"
     CDS             complement(2972..3070)
                     /locus_tag="JN749_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HAY7370295.1"
                     /translation="MISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT         1002 a          772 c          671 g          935 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagactatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt gggaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaataccag acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aaagtgtaca accaccaata tacagcgtca
      661 ggccaattaa aattgccacc ttataactgg tctttttaat cacaagggat gctggtattg
      721 caattaaaaa ataacctcca taaaatgcgc tctgcaccaa tgctgaagca aagttactta
      781 gcgaaaatac acttttgaat tgagtgatta atatgtcatt taatgcagct gcgcatcccc
      841 atagcgggaa taaacacgat aacaaaataa actggaacaa gggagtctta ttcagatacc
      901 catcaggcat ctgaatgatg tttttatcgt tcatagtgct acctttaact gtgcaggatg
      961 attattcgtt taaggttaaa aattcattaa attgttcaat actcggataa gatgattgca
     1021 tacctttccc tgtgacgctg aaagcggcaa agagagcggc ttttttcaaa gcggcttcaa
     1081 catcaccgct ttgaacataa taatgggaaa agcaaccaat aaatgcgtca ccagcgccac
     1141 tagtatcaac agcatttact ttgaatgcag gaacatggac ttcctgatcg cgggtcatcc
     1201 ataatgcgcc tttttcgctc atggtaacaa taatattgtt cagcccttta tcaactaacg
     1261 aacgtgcggc caaacgaata tgatcataag tatcaaccga cataccagtt aatatttcca
     1321 gttctgtttc attcgggata aagaaatcac ctttgcaggc ataagacata tctaactcac
     1381 gcaatgccgg agccggattt aataacactt caataccatt tttcttacca aactcaatcg
     1441 cgtggtaaac tgtttccagt tgaacttcca gttgtaaaac gatcaatttg cattttttca
     1501 gatcttctgc agctcgatcg atatcttccg gggaaagaaa tttattcgct cccttaatta
     1561 ttaatatact attgctcgag ttggcattaa caaagatcgg tgcaacacca ctgctggtac
     1621 aggggacctt ctcaacataa gtggtattaa ttccccatga ttcaagatta cgaatagtat
     1681 tatccgcaaa aatatcatca cctactttag tcagcatcag gacttttgaa ttcaatttag
     1741 ccgccgccac cgcttgatta gcacctttcc caccacatcc gattttgaag gcaggtgctt
     1801 ccagagtttc tccttcttta ggcatctgat tagtgtaagt aatgagatcc accatattgg
     1861 aaccaataac tgcaatgtcc atttcactac ctcttataaa ctttcgcata acaatggtat
     1921 ttaaataaca ttagcatgtt acttttgcat catttgtgac tgagatcgcg attagcacat
     1981 caacccgatg tttatttaat agacttccag tctcatcact caggccaaca ctatctaatc
     2041 ataagcaacc taacaagatt agtgcccaaa attcagcagc ctataccctt ttcatttcaa
     2101 aggggcggtc gtatagtatg gtaatgaaaa caatgtttac taacgccaaa atgttatttt
     2161 tataacattc ttacggagag agagttgatg gaaacgaagc aaaaagagcg tatccgacgt
     2221 ttgatggaac tgcttaagaa aaccgacaga atccatttga aagacgcagc gcgaatgctg
     2281 gaagtttctg taatgactat tcgtcgcgat ctccatcagg aagatgaacc tctgccactg
     2341 accctactgg gtggctatat tgtaatggtg aataaacccg cgctatccat gccagtaatc
     2401 catgacgttc caaaaaatca tcgtgatgac ttacctattg caattctggc tgccggaatg
     2461 gttaatgaaa atgatctgat cttctttgat aatggccagg agataccact cgttataagc
     2521 atgatcccgg atgcaatcac cttcaccggc atctgttact cacatcgcgt ctttgttgcg
     2581 ttgaatgaaa agcctaatgt aacagcaata ctttgtggtg gtacgtatcg tgccagaagt
     2641 gatgcttttt acgatgccag taactcttcg ccattagact ctctcaatcc gcgaaaaata
     2701 tttatttccg ccagcggtgt gcataatcac tttggcgtca gctggtttaa ccctgaagat
     2761 cttgccacta agcgtaaagc gatgaaccgt ggactacgga aaattttgct cgcccgccac
     2821 gcgttgttcg atgaagtggc ctctgccagc ctcgcaccga tctctgcatt tgacgttctg
     2881 attagcgatc gtccgttacc ggcagattat gttacgcact gccagaatgg ttctgtaaag
     2941 atcattacac ctgattcaga agacgaatga cttactgaaa aaacaccaca atcttgttaa
     3001 acatcgtcgg gttggactga ttacgttgca ctttcaccac atattccagc ttatctattt
     3061 ggcttatcac ctactccaga cgctggtcat ccttgaccag tagccagata tggcttttgt
     3121 cgcagtcctg aatcggcagg cagagaatgt cttcaacgtt aagagcgcga cgggcaaaag
     3181 cccacaaagg tgagtcatta cacccggatg gtaaacaggc acttccttgt tgtagaagcc
     3241 gttatattgt ccataatgtc tgggttttta atgcatactg ggtaacaacc ggacgaatct
     3301 tcgaatatca ctggcgctgc cagccggttt gaattcatct cacaaccctg catagagcga
     3361 atctcttgcc tgcacgtcac
//
LOCUS       DADLTF010000257         3319 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.262, whole genome shotgun sequence.
ACCESSION   DADLTF010000257 DADLTF010000000
VERSION     DADLTF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.262"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(368..541)
                     /locus_tag="JN749_004109"
     CDS             complement(368..541)
                     /locus_tag="JN749_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7370296.1"
                     /translation="MKRLQVFKFRLRPGGQQAREMRRFAGACRFVFSRTLARQNENHK
                     AGNKDIPYAKMAS"
     gene            609..1224
                     /gene="tnpA"
                     /locus_tag="JN749_004110"
                     /pseudo
     CDS             609..1224
                     /gene="tnpA"
                     /locus_tag="JN749_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1552..2634
                     /locus_tag="JN749_004111"
     CDS             1552..2634
                     /locus_tag="JN749_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HAY7370297.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKVNL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3123..>3319)
                     /locus_tag="JN749_004112"
     CDS             complement(3123..>3319)
                     /locus_tag="JN749_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7370298.1"
                     /translation="EGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRFT
                     ERFHNRFKITPRELRKASRE"
BASE COUNT          959 a          735 c          672 g          953 t
ORIGIN      
        1 cgaccattga tgctgaaggg tgaaccggag ttgatacttc actttctgcc tgaatactga
       61 tgtaccactt accgcaggac tggctgacag tgacattttt cacaacaccc gtgacctgac
      121 ggctgttccg gtagcgcatc cagccaagtt ttggcagaaa aatacggctg ttttcctgat
      181 cgagcttaac gccctgcgga tagcggaatg catcattctg tcaccgcttt ttgaatcagg
      241 ggaacgccga ccgcttctgg aaaaagtttt tgcaggcctg ctcagggtct ttcagtgact
      301 gttgcaatgg ctgtgacgga gcatctttaa tccattgcgt ttcagtggca tttttccact
      361 caaccaatca ggaagccatt ttcgcgtaag ggatgtcttt gttaccggcc ttatgattct
      421 cattctgacg tgccagagta cgactgaaaa cgaaacgaca tgcgcctgcg aagcgcctca
      481 tttcacgcgc ctgttgacca ccgggtctta accggaattt gaatacctgt agtcgtttca
      541 cagggcaaat tatactttgg tctgtgaaaa aaactgatat ccgacgcgga aggcactgtg
      601 ttttcctgat gcatgctcac cgggtctttg ttaccaagta ccgacgccag atttttgacc
      661 atgacgcgac agaaaaacta cgtacttact tttcaaatgt atgtgctgat tttgaagctg
      721 aactggttga aatggatggc gaaccagagc acgtccatct gttaattaac tatcccccca
      781 aactggcgat atccagtctg gtaaacaaac agcctcaaag gcgtatcggg taggttactg
      841 cgacgggatc ggccagatat tgctgccagg tattaccgca aagatgtttt gtggcgtccc
      901 ggctattttg ccagtagctg cggaggtgcg ccaatatccg tcatccgcca atacattgaa
      961 caacagcaaa cacctggtca ggtgaaaaaa cgagccttat atccccgccc tgaaggacgg
     1021 gcttttacgg tgcactggat aagcctggag attatctaca cctgcatgga catcattgta
     1081 ctatgccccg tacatgcccc atcacatcct caacatcaaa gtattagagc cttgcaaacc
     1141 attatgcgtc tcagtttgtc tcatctatct tacgcaaaaa aaatagaact aatatcaagc
     1201 ggcgctatat tgcgtattaa ataaaaccac ctcaattaca ttaataaatc taaataaata
     1261 tcatacaaat aatttccatg gttattttta ggctatttaa attaaaaaaa taaattatga
     1321 gtcaatcata aataacaacc tttaatataa tttgactacc aaatttatat tcgttcacaa
     1381 aatagtcatg cattgtgtgc aatagaaaca gttcagataa aaatagggat ctacttcaca
     1441 atttaaatga gaaactaaaa cttacatctt gaaataatct cattgattgg atgaatattt
     1501 gtagtgcagt gacatcattt ttaaataata gttcaaataa aagggctcac gatgaaaaaa
     1561 ttaacagtgg caatttctgc tgtagctgca tcagtactga tggcgatgtc tgctcaggca
     1621 gcagagattt acaataaaga cagtaacaag ctggatctgt acggaaaagt taatgctaag
     1681 cactacttct cttctaacga tgcagatgat ggcgacacaa cttatgttcg tctgggcttc
     1741 aaaggcgaaa ctcaaatcaa cgatcaactg actggtttcg gtcagtggga atatgaattc
     1801 aaaggcaacc gtgctgaatc tcaaggttct tccaaagata aaacccgtct tgccttcgct
     1861 ggcctgaaat tcggtgacta cggctccatc gattatggcc gtaactacgg tgtagcatac
     1921 gatatcggtg catggaccga cgttctgcca gaattcggtg gcgatacctg gacccaaaca
     1981 gatgtgttca tgactggtcg caccacaggt gttgcaacct atcgtaacaa cgacttcttt
     2041 ggtctggttg atggtctgaa ctttgctgct cagtatcagg gtaaaaatga ccgcactgac
     2101 gtaactgaag ctaacggtga tggtttcggt ttctccacta cttatgagta tgaaggattc
     2161 ggtgtaggtg caacctatgc taaatctgac cgcactaatg atcaggttat ctacggtaac
     2221 aacagcctga atgcatctgg tcaaaatgct gaagtatggg cagctggtct gaaatatgat
     2281 gcgaacaaca tctatctggc taccacctat tctgaaaccc agaacatgac tgtttttggt
     2341 aataaccata ttgccaacaa agcacaaaac ttcgaagtag ttgcacaata tcagttcgac
     2401 ttcggtctgc gtccgtccgt tgcttacctg caatctaaag gaaaagactt gggtgcgtgg
     2461 ggtgatcagg acctgattga atatattgat gtaggtgcaa cctattactt caacaaaaat
     2521 atgtccactt ttgttgatta caaagtcaac ctgattgata agagcgattt cacgaaagca
     2581 tctggcgttg ctaccgatga tatcgttgct gtaggattgg tttaccagtt ctaatctgat
     2641 tactcaaaga tatgttgtgg gaggctttgc ctccccaaca tataagtggc gccctcaagc
     2701 cactttcctt agaagcacta tcttgcttct gactatataa attacccttt attttctggg
     2761 acgtttcaac gccccatttt taataatact cagtgaacaa ttaacgtatc aattagattt
     2821 aataacaacg atatccgtac ctaacctgat atctaatgtc ataagcatcc ctttaattat
     2881 cccctcaacc tcttgtaaat ttcccactct tgagctccac atagtccaaa cgccggatgc
     2941 aaaaaaaatc acacgtgcat ctctgaaatt gtgatggagg aagtgagtat caattatatg
     3001 tgcttataaa cgttcccgat tggcacagaa gtcaccggat tgttcaggct ccaataggtt
     3061 gagtatgtcg tatttatttt ccaaaataaa aatcgatgct gttgctcctt aataaaatac
     3121 ttttactctc tggacgcttt tcttagctct cttggtgtta tcttaaaacg attatggaac
     3181 ctttcagtaa aacgagaagg acacttgtaa ccattttccc tggcaatctc gctgatgggt
     3241 tttactgtcg tttgtatagc agacaacgca ttatttaacc tcacgtcgtc caatatactt
     3301 tgaaaactta ttccctcgc
//
LOCUS       DADLTF010000258         3254 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.263, whole genome shotgun sequence.
ACCESSION   DADLTF010000258 DADLTF010000000
VERSION     DADLTF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.263"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN749_004113"
     CDS             complement(<1..203)
                     /gene="mokC"
                     /locus_tag="JN749_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HAY7370299.1"
                     /translation="MLNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYES"
     gene            732..1898
                     /gene="nhaA"
                     /locus_tag="JN749_004114"
     CDS             732..1898
                     /gene="nhaA"
                     /locus_tag="JN749_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HAY7370300.1"
                     /translation="MKHLHQFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1964..2863
                     /gene="nhaR"
                     /locus_tag="JN749_004115"
     CDS             1964..2863
                     /gene="nhaR"
                     /locus_tag="JN749_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HAY7370301.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(2901..>3254)
                     /locus_tag="JN749_004116"
     CDS             complement(2901..>3254)
                     /locus_tag="JN749_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370302.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          764 a          736 c          793 g          961 t
ORIGIN      
        1 gattcgtaag ccgtgaaaac agcaacctcc gtctggccag ttcggatgtg aacctcacag
       61 aggtcttttc tcgtcaccag cgccgccact acggcggtga tacagatgac gatcagggcg
      121 acaatcatcg ccttatgctg cttcattgct ctcttctcct tgaccttacg gtcagtaaga
      181 ggcactctac atgtgttcag catataggag gcctcgggtt gatggtaaaa tatcactcgg
      241 ggcttttctc tatctgccgt tcagctaatg cctgagacag acagcctcaa gcacccgccg
      301 ctattatatc gctctcttta acccattttg ttttatcgat tctaatcctg aagacgcctc
      361 gcatttttgt ggcgtaattt tttaatgatt taattattta actttaattt atctcttcat
      421 cgcaattatt gacgacaagc tggattattt ttgaaatatt ggcctaacaa gcatcgccga
      481 ctgacaacaa attaattatt acttttccta attaatccct caggaatcct caccttaagc
      541 tatgattatc tgggcttagg gtcactcgtg agcgcttaca gccgtcaaaa acgcatctca
      601 ccgctgatgg cgcaaattct tcaatagctc gtaaaaaacg aattattcct acactataat
      661 ctgattttaa cgatgattcg tgcggggtaa aatagtaaaa acgatctatt cacctgaaag
      721 agaaataaaa agtgaaacat ctgcatcaat tctttagcag tgatgcctcg ggaggcatta
      781 ttcttatcat tgccgctatc ctggcgatga ttatggccaa cagcggcgca accagtggat
      841 ggtatcacga ctttctggag acgccggttc agctccgggt tggttcactc gaaatcaaca
      901 aaaacatgct gttatggata aatgacgcgc tgatggcggt atttttcctg ttagtcggtc
      961 tggaagttaa acgtgaactg atgcaaggat cgctagccag cttacgccag gccgcatttc
     1021 cagttatcgc cgctattggt gggatgattg tgccggcatt actctatctg ccttttaact
     1081 atgccgatcc gattacccgc gaagggtggg cgatcccggc ggctactgac attgcttttg
     1141 cacttggtgt actggcgctg ttgggaagtc gtgttccgtt agcgctgaag atctttttga
     1201 tggctctggc tattatcgac gatcttgggg ccatcattat catcgcattg ttctacacta
     1261 atgacttatc gatggcctct cttggcgtcg cggctgtagc aattgcggta ctcgcggtat
     1321 tgaatctgtg tggtgtacgc cgcacgggcg tctatattct tgttggcgtg gtgttgtgga
     1381 ctgcggtgtt gaaatcgggg gttcacgcaa ctctggcggg ggtaattgtc ggcttcttta
     1441 ttcctttgaa agagaagcat gggcgttctc cagcgaagcg actggagcat gtgttgcacc
     1501 cgtgggtggc gtatctgatt ttgccgctgt ttgcattttc taatgctggc gtttcactgc
     1561 aaggcgtcac gctggatggc ttgacctcca ttctgccatt ggggatcatc gctggcttgc
     1621 tgattggcaa accgctgggg attagtctgt tctgctggtt ggcgctgcgt ttgaaactgg
     1681 cgcatctgcc tgagggaacg acttatcagc aaattatggt ggtggggatc ctgtgcggta
     1741 tcggttttac tatgtctatc tttattgcca gcctggcctt tggtagcgta gatccagaac
     1801 tgattaactg ggcgaaactc ggtatcctgg tcggttctat ctcttcggcg gtaattggat
     1861 acagctggtt acgcgttcgt ttgcgtccat cagtttgaca ggacggttta ccggggagcc
     1921 ataaacggct cccttttcat tgttatcagg gagagaaatg agcatgtctc atatcaatta
     1981 caaccacttg tattacttct ggcatgtcta taaagaaggt tccgtggttg gcgcagcgga
     2041 ggcgctttat ttaactccac aaaccattac cggacagatt cgagcgctgg aagagcgcct
     2101 gcaaggcaaa ttatttaaac gcaagggacg tggtctcgaa cccagcgagc tgggagaact
     2161 ggtctatcgc tatgccgata aaatgttcac cttaagccag gaaatgctgg atattgtgaa
     2221 ctatcgcaaa gaatccaatt tattgtttga cgttggcgtg gctgatgcac tttccaaacg
     2281 cctggtcagt agcgtactta acgccgcagt ggtagaaggc gagcccattc atcttcgctg
     2341 cttcgaatcc acccacgaaa tgctgctgga gcaattaagt cagcataaac tggatatgat
     2401 catttctgac tgtccgatag actctacgca gcaggaaggc ctgttctccg tgagaattgg
     2461 cgaatgtggc gtgagtttct ggtgtacaaa tccaccacca gaaaaaccgt tcccggcttg
     2521 tctggaagaa cggcgacttt tgattcctgg gcgacgttca atgttagggc gcaaattgct
     2581 taactggttt aactcccagg gattaaacgt agaaatcctc ggcgagtttg atgatgccgc
     2641 tttgatgaaa gcttttggtg cgatgcacaa tgcaatcttc gttgccccaa cgctttatgc
     2701 atatgatttt tatgccgata aaactgtcgt agaaattggt cgcgtcgaga atgtgatgga
     2761 agagtaccat gctatttttg ctgagcggat gattcagcac ccggcggtac agcgaatctg
     2821 caatacggat tattctgcgc tttttagtcc agcggtgcgt taatcggcag ctcccccaaa
     2881 gttaaggtgg gggagataga ttagttgtac attaccacga ttttgactcg gctcattatt
     2941 tgcccgcttg agacattgtt tccatatgta cgcgggcgaa taaatagagg aatctgatta
     3001 cttccttcat ggggatgctg aaaagagtag taattgcttt taccatactg gatttcattc
     3061 gtaccatcat tggagagttg aaagcctatt tgacccgcac cgtcctcact gttttctaac
     3121 tcatttttta atatgctggt tcctgactcc tgtatcgtca ctggttcaaa gtaatattca
     3181 acgtcactgg gttcaaaaac taattcacct tgctcgccct ggatgcagtc aaatcttata
     3241 tgatgaaact gata
//
LOCUS       DADLTF010000259         3214 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.264, whole genome shotgun sequence.
ACCESSION   DADLTF010000259 DADLTF010000000
VERSION     DADLTF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.264"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            368..2020
                     /locus_tag="JN749_004117"
     CDS             368..2020
                     /locus_tag="JN749_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HAY7370303.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="JN749_004118"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="JN749_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2280..2393)
                     /locus_tag="JN749_004119"
                     /pseudo
     CDS             complement(2280..2393)
                     /locus_tag="JN749_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989794.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..2853)
                     /locus_tag="JN749_004120"
     CDS             complement(2527..2853)
                     /locus_tag="JN749_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7370304.1"
                     /translation="MPGASNAAMCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWI
                     EYYGKFWSRNFSYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLALVRKQYPKLFAHWYL
                     LRASNE"
BASE COUNT          927 a          697 c          725 g          865 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctctcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaa
//
LOCUS       DADLTF010000260         3177 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.265, whole genome shotgun sequence.
ACCESSION   DADLTF010000260 DADLTF010000000
VERSION     DADLTF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.265"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            847..1041
                     /locus_tag="JN749_004121"
     CDS             847..1041
                     /locus_tag="JN749_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HAY7370305.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESEIGT"
     gene            1228..1662
                     /locus_tag="JN749_004122"
                     /pseudo
     CDS             1228..1662
                     /locus_tag="JN749_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532398.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiation protein"
     gene            1687..1821
                     /locus_tag="JN749_004123"
     CDS             1687..1821
                     /locus_tag="JN749_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAY7370306.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="JN749_004124"
     CDS             complement(2066..3175)
                     /gene="fepE"
                     /locus_tag="JN749_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HAY7370307.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          941 a          782 c          657 g          797 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagata tttaacataa tatcttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccctaatata agatgatgtt tttttatgaa
      421 aatagtcagc ccccccccct aaaaagcggt gttggcgcag ctacaacacc gcgccgacac
      481 cgctttttta aatatcacaa agagagcaag aagaactaat ttgtcataac tctctattta
      541 taaagaaaaa tcagcaaaaa cttgtttttg cgtggggtgt gggcatttca ggcatgagaa
      601 cagactcgcg tttttccgga gtatcagcgg aattattgat taaggaacac tcgacgcaga
      661 gcgaaatttt atgcaacagc ggctgcactg cacagggatt atttatcagt tgatatgctg
      721 tgccatgata cgtcctaaag aaacaggaca ggatgtgcaa ttgtaatacc gtcgcccgca
      781 acgtcttaca attaccacct gctcagcccc cccccgacac ccggaactga cggagaacaa
      841 cggaaggtga acaaacaaca acaaactgcg ctgaatatgg cgcgatgtat caaaagccag
      901 agcctgacac tgcttgaaaa actggatgct ctggatgccg acgaacaggc ggccatgtgc
      961 aaacgactgc acgaactcgc agaagaactc cagaacagca tccagattcg ctttgaggcc
     1021 gaaagtgaaa taggaacata acgaagctga cggaggcggt cacggctttc ccgtgggcgt
     1081 tttcgtcagc agacaagccc gtcagggcgc gtcagggggt tttagcaggt gtcggggcgc
     1141 agccatgacc cagtcacgta gcgatagcgg agtatatact ggcttaatca tgcggcggca
     1201 gagcaaatgg tgagagaaca gcgacatatg aggtgtgaaa tgctgcacag atacgtaagg
     1261 agaaaatgca gcttccggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1321 gttcggctgc ggcgagcggt gtctgctcac gcaaagacgg atatacggtt atccacagaa
     1381 tcaggggata acaccagaaa aaacatgtga gcaaaaaaga ggccccgaaa aaaggccgcg
     1441 ccggtggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa ccgacgctca
     1501 agtcagaggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct
     1561 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc
     1621 cgcgttcgtc tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg
     1681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1741 cttgagtcca acccggtaag acacgactta acgccattgg cagcggccac tggtaaacag
     1801 gatgtgcgag agatgtattg agagttcttg aagtggcagc cttactgagg ctacactaga
     1861 aggacagatt tggtgacatg tctcgcacaa gactgttacc acggttcaga agttccccaa
     1921 cttactgaac cttcgaaaaa ccacctcccc aggtggtttt ttcgttttct agagtagaag
     1981 actacggctg taagggaatg atctcaagaa gatcctttga actttttata aaagttgctt
     2041 ataaaatgat gcatcagcac aacttttact caatcacact catctttctg gcctccatgg
     2101 catgacgtaa aagtatatag ccacatgcaa gcatccctcc aatgacagaa gaaagaatta
     2161 caattatggc attacttggc ccatcctttc tgaccggtaa cgttgggctc aattgatact
     2221 gaaatggcat aaaatcaaca tcccccactt tcgccacaac caattgattc acaacatatt
     2281 gacgatttcg caactcccca ctcaattccg aaacatcctt gattgatttt ttgatgttca
     2341 atttagttgc tataccatca gaaccgagag ccacaggaaa atctgggtca tccttcataa
     2401 tctgaccatt gctgtataca ggttttttta ttccagccgc attagcaacc tccagtgaat
     2461 aattgaggcg tcgaatatct gcattcagct ggtttctaat ttttatctca tccagtgcaa
     2521 gaatttcttt ttcaacatta gttttaactt ctagtttatt tcttatatct tccatcaaat
     2581 cccttacaac aagggaagaa acataattaa tatacccaga caacacatcg tgagcttctt
     2641 tacttgttgg agcagtaaaa ctcagagtcc atgatacata caatgatgac ttgtcgtttt
     2701 tcttactggc attatcatca atcgacttca tatttttact caactcaaca attgcacgat
     2761 gcagttcgag agcgctaatg ttattctttt taataatctc cataacataa ggagatgatc
     2821 tcatatactc attcaacgaa ttaacggact gaaatttctt aataaacaga gcaaatgcgc
     2881 tatcacgatt gatatcaaaa tccactccca gaacatgaag cttggaaagc tccttattta
     2941 attcactcca ttgaacagac tcagcaggag taaccacagc agaactagtc catttttgtg
     3001 gcaaaacaaa agaaacaaga aagccaatac aagcaaaaaa tagaatcacc ccaacaattt
     3061 ttatttttgc tgcccataaa acgctaatca agccaaaaag gtcaatttct tcattaccat
     3121 tcggcaacat gttgtaatta gaaaaatgtt cgtcagagac ctttgagatt ttcattg
//
LOCUS       DADLTF010000261         3142 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.266, whole genome shotgun sequence.
ACCESSION   DADLTF010000261 DADLTF010000000
VERSION     DADLTF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.266"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JN749_004125"
     CDS             complement(<1..740)
                     /gene="ipaH9.8"
                     /locus_tag="JN749_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HAY7370308.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQ"
     gene            complement(1002..1097)
                     /locus_tag="JN749_004126"
                     /pseudo
     CDS             complement(1002..1097)
                     /locus_tag="JN749_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JN749_004127"
     CDS             complement(1133..1438)
                     /gene="ccdB"
                     /locus_tag="JN749_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HAY7370309.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JN749_004128"
     CDS             complement(1440..1658)
                     /gene="ccdA"
                     /locus_tag="JN749_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HAY7370310.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2194..3142)
                     /locus_tag="JN749_004129"
                     /pseudo
     CDS             complement(2194..3142)
                     /locus_tag="JN749_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          781 a          762 c          762 g          837 t
ORIGIN      
        1 tgttgtccgt cactcatgga gaagtaaatc cgtgggccgt ggtagtccgg cgaagaggtt
       61 aatctttgca gggcttgcag agcatgggat gataatgggt tatcactaat atgtattgaa
      121 cattcattcc tcagattaag aatgctttcc gggatatgac ttatctgatt tctatcaaaa
      181 aaatactctc tgacaacggg attatttccc tcagaaaatg ctggtagctc agtgaggaaa
      241 ttacgggtcg ctctgagatt tttaagagcc tgtgggagcg atggtaagga gactatttcg
      301 ttatagctga tattcatagt caataacgaa tcgggtaaag ctggcaggtt ttccagctca
      361 ttgtgttgta cctgaagtga ctccagcgca ggaggtagca cgggcaattc tgataattta
      421 ttgctggcgg aatataattt ttttagcgta acaggcagtt caggtaggtt agttaattga
      481 ttatatgata cattgagcag cgttatctga gctggtaagt tgtcaggtag cgaggacaga
      541 tttaaacggt ccagtcgaag ttcatcggaa ttattgataa gacattcttt aagtcgggag
      601 acagcctcat cacgctcttc accggggagc gcttgttttt cccatttatc ccatgctgaa
      661 aagtaatcag catatgtacc ggaaatagtg ttataaaaag aattttgggg caatgaaaag
      721 ttattattta tcggtaacat gatgggcatc cagttaaagc cggtggacaa atggctttgt
      781 taaaagaaat aagtaggagt ttttatgaag ttcattccat gaaatgaact tcagcacttt
      841 tttattataa taaggataaa caaaattggc tgtaaaaaaa cgatactgtt tcagttaacc
      901 gtgaatggcc tgtttgcacc gtattgttct tgtttttcgg ttagttaaac atcgtacttc
      961 atatttgaac ggtaagcggc tcgtcagaac cgtattgata tttactgaga ggtcagatca
     1021 actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat atgctcaatg
     1081 acgtaacgca gccattttgt caacgccacg atttttgacc ctagcctgac atttatattc
     1141 cccagaacat caggttaatg gcgtttttga tgtcattttc gcggtggctg agatcagcca
     1201 cttcttcccc gataacgaag atcggcacac tggccatatc agtggtcatc atgcgccagc
     1261 tttcatcccc gacatgcacc accgggtaaa gctcacggga gactttatct gacagcaggc
     1321 gggcactggc cagggggatc accatccgtc gtccgggcgt gtcaataatg tcactctgta
     1381 catccacaaa cagacgataa cgactctctc ttttataggc gtaaacctta aactgcattt
     1441 caccagtccc tattctcatc ggcaaaagag ccgttcattt caataaaacg ggcgatctca
     1501 gccatcccct gctgattttc agcctgccag cgttccgcac gcagacggcg ggcctctttt
     1561 tgcatggccg tatttaccag gcctgagata ttcacgtttg cggatttaag taactgatag
     1621 ctgtcactgt ctatagtgac agtaatacgc tgcttcatga taagccctca aaaacatact
     1681 tatgatatgt atatgagtat atattaatgg tacaaaccat caatatccat aaaaagtaca
     1741 ccattaattc gctataagtt tttaatacaa ttttttctgc tgcgattttt ttatactata
     1801 attagcatac ttataacacc gtaagtaaat tacatgaaag caggcacagc gtgatccgat
     1861 ttttctgcgt ataacgattt tccggaagtg taatacggtt gcgccccggg ccgggcaccc
     1921 ttttctgttg ttgtgtacgg gcccacagag aatggtaccg gaggcgggct cagaagtact
     1981 gagtcgggtg atcgcgtcag gccgcaaagt actgacacgg gtattggtga ctggtgatga
     2041 agtactgccc cgggtatgat ttcaggctga ttcaggttct gattctctgt cagtccgcgt
     2101 ggttgctggc ctggctgatt tcctgttgtc aggcgttcaa caaccgactg caggttatag
     2161 cccggttcac gaacacctga cggcaggtat cactcacccg gtgaaggtaa atccctcaag
     2221 aggcagcaac tctgccagtc gctcctccgg ccattccggc agacgcgtca ggacgtccgt
     2281 cagccaggca tgtggctcca gaccattacg ttttgctgtt tccagaaggc tcattatctg
     2341 tgccgcccgg tttccggcca tcagtgaacc ggcgaacagc cacgctttcc gtcccataac
     2401 aaccgggcga atggcacgct cgcagatatt gttgtccagg ggcaccgctc cgtcacccag
     2461 gaagcagctc agttcatcgc gccggttcag aatatagtta atcgccttat ttaatggccc
     2521 gtcgggcgga cagcacggct cctgctcttc aagccaggca aacaggtcat tcactatcgg
     2581 ccgggagtac cgttatcgcc actgccgtat tttttccaca gggcgctcac ggatgagttt
     2641 ctcaatgcgg taaagacccg cgatttttct cagctccacg cctgcacggg gctccctgct
     2701 ggccttatac agatttgcga agccgcgccc tgcgtgactc cagcaacagg ctgatgtgat
     2761 gccggggtga ttatccgcca gagatttgta cacgctgtat ccgtcactca ccagcgtacc
     2821 gccacaccag cactgcagcc aggtctcagg gtatctgagg gcccgtccgg tctgcgagtc
     2881 aaaacagacg accggcggac cgcttctttc accgctgacg taagcacaca gccagccgga
     2941 gcaggattta ccgcctttcc gggtatcaag gagcctcagg gacgtttcat ctgcatgaat
     3001 aacatcccgt gtcagaagct catggcgtag cagtttcgcc agcggggcca gtgcagctcc
     3061 ggcaacaccg accatatccg ccattgtact gacggggagc tccacaccca ttcgggagaa
     3121 aatgtgctgc tggcgataca gg
//
LOCUS       DADLTF010000262         3137 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.267, whole genome shotgun sequence.
ACCESSION   DADLTF010000262 DADLTF010000000
VERSION     DADLTF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.267"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..71)
                     /locus_tag="JN749_004130"
     CDS             complement(<1..71)
                     /locus_tag="JN749_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7370311.1"
                     /translation="MASISISCPSCSATEGVVRNGKST"
     gene            263..1240
                     /locus_tag="JN749_004131"
     CDS             263..1240
                     /locus_tag="JN749_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000600827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7370312.1"
                     /translation="MILIPDDEPELSLPAASEEFLPALSGENAPVSPARAYLLSLNSH
                     RSRQTMASFLNIVAVMLGAASLESCSWGSLRRHHVMAVTELLRDTGRATATVNTYLSA
                     LKGVAKEAWMLRLMDVESFQHIRAVRNLRGSRLPSGRALPQGEIRALFAVCEADRSCL
                     GARDAAMLAVILGCGLRRSEVVSLDLRDVVTQDRALRVLGKGNKERLAYVPAGAWQRL
                     QIWIDEIRGETPGPLFTRIRRFGDVTLNRLTDQAVYHILQVRQGQAGITKCSPHDLRR
                     TFATAMLDNGEDLITVKDAMGHASVTTTQQYDRRGEQRLQDARDRLNLI"
     gene            complement(1288..1551)
                     /locus_tag="JN749_004132"
                     /pseudo
     CDS             complement(1288..1551)
                     /locus_tag="JN749_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004193081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
     gene            complement(1586..>3137)
                     /locus_tag="JN749_004133"
     CDS             complement(1586..>3137)
                     /locus_tag="JN749_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HAY7370313.1"
                     /translation="DIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQYL
                     EERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQLLP
                     RIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESSPG
                     LTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGGRG
                     ESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDSTYVLDGLLYHESDLRIE
                     EHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGVQAYPTLRPLIGGTLNI
                     KHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRIERTLFILD
                     WLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAI
                     VLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTGDYVWRQSRRLEDGKFR
                     PLRMPGKP"
BASE COUNT          596 a          950 c          988 g          603 t
ORIGIN      
        1 gtacttttac cgttacgcac cacgccttca gtagctgagc aggagggaca actgatggag
       61 atggaagcca cgggagcacc tcaaaaccac catcatacac taaatcagta agttggcagc
      121 atcaccgtaa tcgtacagct aagaacaagt gcttcaagtc tacttattaa aatgtacctt
      181 tgaaaaagta gactttctgc ggtacgctct gacagtaagt ttgcccccga ttttttacgc
      241 agcgcgccgg acggagcccg acatgatcct gattcccgat gacgaaccgg agctgagtct
      301 gcccgcagcc agtgaggaat tcctgccggc gctgtcaggt gagaatgccc cggtcagccc
      361 ggcccgggcc tacctgcttt cactcaattc ccaccgcagc cggcagacca tggcctcgtt
      421 cctgaacatc gtcgccgtta tgctcggagc tgcctccctg gagtcctgca gctggggcag
      481 cctgcggcgc catcacgtga tggccgtgac cgaactgctg cgcgacaccg gccgggccac
      541 cgccaccgtc aacacctatc tttcggcact caagggcgtg gcgaaggaag cctggatgct
      601 ccggctcatg gatgtggaga gcttccagca tatccgggcg gtccgtaacc tgcgcggcag
      661 tcggctgccc agcggtcggg cgctgcccca gggggagatc cgcgccctgt ttgccgtctg
      721 tgaagccgat cgcagctgcc tcggggcgcg ggatgcagcg atgctggcgg tcattctcgg
      781 ctgcggcctg cgacgatccg aagtagtgag cctggatttg cgtgacgttg tcactcagga
      841 ccgtgcgctt agagtgctgg gtaagggaaa caaggagcga ctggcatatg tcccggccgg
      901 tgcctggcag cggctgcaga tctggatcga tgagatccgg ggcgagaccc cgggcccgct
      961 gttcacacgc atccgtcgtt tcggggatgt gactctaaac cggctaaccg accaggcggt
     1021 gtaccatatc ctgcaggtgc gccagggtca ggccggcatc acgaaatgtt ctccacacga
     1081 tctgaggcga accttcgcca ccgcgatgct ggataacggg gaggatttaa ttaccgtgaa
     1141 ggatgcgatg ggccacgcga gcgtcaccac cacccagcag tatgaccgtc gcggggagca
     1201 acgcctgcag gacgcgcggg atagactcaa ccttatttag ggctaagtaa acacaaacaa
     1261 gggggctgta ccccctctaa ttttacatta acacgatact cgacagactt tcccagtcgt
     1321 agtcagcaac catcgcccga tggaggctgg gataaatgct gacagcccga cgtgaagcaa
     1381 aaggctctcg ccgctggcat ggcttaatgt cgcggtcccc tcaatcacta acacaatttc
     1441 agcgctggat gtcattaccg tttgattgtt caccgcagtg aaaatactga aatggaaatc
     1501 ctcaacagga ataggatagc gcgcttctgc ggcgtgctgt tccggtaaca taggggagcc
     1561 cgcagaattc ggaaaaaatc gtacgctaag gttttccggg catccgtaag ggccgaaact
     1621 tcccgtcttc cagtctgcgg ctctgccgcc agacgtaatc gccggttagg ttgatgtgct
     1681 cccagcccag cggcgacagg aattgcagca gctcgccgtc caccggcttg ccggcctcga
     1741 ccaacccctg ggtggcgcgt tccaggtaca ccgtgttcca cagcacgata gccgccgtca
     1801 ccaggttgag gccgctggcc cggtagcgct gctgctcgaa gctccgatcc ctgatttccc
     1861 caaggcggtt gaagaacacc gccctggcca gcgagttgcg cgcctcacct ttgttcaggc
     1921 cggcatgcac gcggcggcgc agttcaacac tttgcagcca gtccaggatg aacagcgtgc
     1981 gctcgatccg gcccagctcg cgcagggcca cggccagtcc gttctggcgc gggtagctgc
     2041 cgagcttgcg cagcatcagc gaggcggtga cggtgccctg cttgatcgag ctggccaggc
     2101 gcaggatgtc gtcccagtgg gcacgcacgt gcttgatgtt cagggtgccg ccgatcagcg
     2161 ggcgcaacgt cgggtaggct tgcacgccct gcggcacgta cagcttggtt tcgccgaggt
     2221 cgcggatgcg cggcgcgaag cggaagccta gcaggtgcat cagggcaaag acgtgatcgg
     2281 tgaagccggc cgtgtcggtg tagtgctcct cgatccgcag gtcggactcg tggtacagca
     2341 ggccgtcgag cacataggtg gaatcgcgga cgccgacatt caccacgcgg gtgctgaacg
     2401 gcgcgtactg gtcggagata tgggtataga acagccgtcc cggctcgcta ccgtacttcg
     2461 ggttgacgtg cccggtgctc tcgccccggc cacccgcgcg gaagcgctgg ccatcggagg
     2521 atgaggtcgt gccgtcgccc cagtgggcgg caaaggcgtg gcgatactgg tggttgacca
     2581 gctcggccaa ggccgccgaa taggtttcgt cgcggatgtg ccaggcttgc agccaggaca
     2641 gcttggcgta ggtcaggccg gggctcgact cggccatctt ggtcagcccg aggttgatcg
     2701 catcaccgag gattgcggac agcagcaacg tcctgtcttt ggcctcggcc ccgtccttca
     2761 agtgggtgaa gtggcggctg aagcccgtcc agtcgtccac gtccatcagc agttcggtga
     2821 tcttgatgcg cggcagtaac tggctggttt ggtcgatcag cgcctgcgcc cgatccggca
     2881 ccgccgcatc cagcggggtg attttcagcc ctgactcggt gaggatggca tcgggcagct
     2941 cgttgtcctt ggccaggcgg gtgacggtgg ccaactgctc gtccagcagc tgcaaacgct
     3001 cttccaggta ctggtcgctg ttcgggttga tcgccagggg cagggcctgc tcgcgcttga
     3061 gtgcggcgaa cttctcggcc ggcagcaggt agtcgtcgaa gtcgcggaac tgccgcgagc
     3121 ccttgaccca gatgtcg
//
LOCUS       DADLTF010000263         3082 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.268, whole genome shotgun sequence.
ACCESSION   DADLTF010000263 DADLTF010000000
VERSION     DADLTF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.268"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(143..871)
                     /locus_tag="JN749_004134"
     CDS             complement(143..871)
                     /locus_tag="JN749_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAY7370314.1"
                     /translation="MSQQEISVINLDQLVSMTSVEIAELTGKEHRNVLRDIRNMAEEL
                     NALKTEQCSKLSSHIGVTEDVYLNAQGKQQPLYRLDRKHTFILVAGYSVHLRAKCYDH
                     IQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKN
                     PISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKMRGILIA
                     FTGTDQQKQEHLLKLAQSGKVEGI"
     gene            complement(1834..2487)
                     /locus_tag="JN749_004135"
     CDS             complement(1834..2487)
                     /locus_tag="JN749_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HAY7370315.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
     gene            complement(2480..>3082)
                     /locus_tag="JN749_004136"
     CDS             complement(2480..>3082)
                     /locus_tag="JN749_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7370316.1"
                     /translation="AKAYSQKNAGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFISLVEDRRKIEGDNAM
                     VFDGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVDRLVNVMAGVYKETGVNG"
BASE COUNT          746 a          786 c          755 g          795 t
ORIGIN      
        1 ggctattagc tcgtctcggg attggtgctg gcgggtctca tcttccgggt ctagaccaag
       61 ctctctgagg aggacttcag tccggtagcc acggtaacgg agagtcatgc gcccctcagg
      121 tgtgcgcggg tcaatcatca tcttagattc cctccacctt accggattgt gccagcttca
      181 ggaggtgctc ttgcttctgc tggtcggtgc cagtgaatgc aataaggatg cctcgcatct
      241 tgtcaaccat ctcaggctta ccgaagacct ccagcccctt agcgttgaac cggaagttat
      301 acccacctgc tgagaacttc tggtctgacg gcttaatgat acgaaagcag tcggaatact
      361 tcggatttac agaccactcg ttactgattg ggttcttacg aaagaggcca cgatgctgga
      421 gaagtgcggt cattagcttc tgagcgggca gaccataggt tttgcagtag tcgccccaag
      481 tcacgcctcg gcggttagcg gactgaatgg ctgctcgctt cttctggtct tccagttgaa
      541 ggactcgacg ctctaacgtc tgaatatggt cgtaacactt ggcgcggagg tgaacggaat
      601 agccagcgac cagaatgaaa gtatgcttac ggtcgagacg atacagcggc tgctgtttcc
      661 cctgcgcgtt tagatagaca tcttcagtca ccccgatatg actgctcagt tttgagcact
      721 gctcagtttt gagcgcgtta agctcctcag ccatattgcg aatatcacgc aggacatttc
      781 ggtgctcctt gcctgtcagc tcagcaattt caactgaggt catggagaca agctggtcga
      841 ggttaatgac gctgatttct tgttgggaca tttacgtgta ctccttttgt ttgcaccctg
      901 aagtcgcacc ctactgacct actcaagccc ccacgtaaag ggagaccagc aggcaacagg
      961 acgcaaacag aatgacttga ggtaattgtg atgtggtctg cgatgaccag tagtgataaa
     1021 ggtgcaagac agcatgtccg acgtccagat ggtgagcggc gatagaccca agtcaaaatg
     1081 aagacacgag aacgctaacg ggctgggagt ctgacagagg ctgtcttgct agccttggta
     1141 tggagaaaga cttaatgtcg gggggcttcc gcataacttg acgcatcaag accagaggcg
     1201 gaatgtatgg cccctatagt gatagctaag ggtccattcc ctatagtgag ggtataagat
     1261 ttgctacctg ataaggccat aatctgacct ccagttagac ccgaagtatg gagaccacat
     1321 catagcgacc atagtttact tcgggatgac aggaggctgg acttaaagtt cttcttattg
     1381 gaattgtgat aaacacaatc cataacaaga taaactcatg gtctcctcca gcctgctggt
     1441 caggactcaa ggttcaacct cagacaccac tggtctaacc tccttgagtt tccgcctgtc
     1501 cacaatggat ggcagacctc agcaatgatg tcttggttct tcacaatctc gcacaccgat
     1561 ggtctcatag acggaagttc cgctgaggtc tgatgttctc cgcctgtcaa aggagagaga
     1621 ttgctacgtt aaagggaatg gtctcaggcc aaggtgattt aacactaggc gtctgcggat
     1681 ggtttccaat cggtccgcta ccggaagcct gagacttaat gaatgtgtgt gggtcgtgaa
     1741 tgttattaag gtggggactt agggtctctc ccctatagtg atagttaagc ccccttccct
     1801 atagtgaggg tatattcaat ctgacctaaa gctttactcg ctagctgcct gctgctgttc
     1861 cctcgccttc ctgtcccggt cctcaatgcc cttgagcgcc ctttccggca tgctgttaaa
     1921 catggcttcg acttctttcg gtctggtaat ctgctcagtg acaataatgt tcagcagctt
     1981 aaaaagacca tgcgctaact gagggtcgtc atcaatatta atttcacccg gatggaccgc
     2041 ctgattgcca acgatacgac aaatatcagc agctcgctgg accaatggag acaatccttt
     2101 ggctacaagc gacttaatgt ccgtgtcgat atgcttcccc ggctcaccga gatggaccat
     2161 cagcttctga agacttaagc gcaggagtgc ggcagcgcct ttaggagaaa ggttaacaat
     2221 gcttctggcc tccatgtagt cggacttaca gtcgtctggc atgtctgggt taggcatctc
     2281 aacgtttgct gacgctggta taatcatggt ttcgtcatac caataagctt tctcattgca
     2341 tcgttcacat ttagaaaagg cgaaaggtgt ctcataaatc ccgttagcgc catattttaa
     2401 gcggacccag cgctgttgag aataagcgcc acaattcggg cagttaaaag cagtgccacc
     2461 aaactctgcc ggataatact cagccattta ctccagtctc cttataaaca cccgccatca
     2521 cattaaccaa tctatctacc tcgacctctt tcgcgtaacg gtcgtaagtt atagacccag
     2581 agctatgacc cataatcgcc tgagcatacg ccaattcgac cccatgttgt tttagctggg
     2641 tcgcaactgt atgtctaaac gagtgaaaag ctaagccgct acggtcagca aggactttag
     2701 gtagcaacgt tcggttaaac catttgctca cctgctcacc gtagccgttc ttcatcagct
     2761 tgaggccatc gaataccata gcattatcac cctcaatctt tctacggtct tctacgagtg
     2821 atataaagtc agccaataca aaaccataag caccatcgac cagcgggaca cagcggtcac
     2881 tatgggcgtt cttgatactc ttacccggca ggctgctatc gtcttcgttg atatggataa
     2941 acacggtccc ggcctcagtg gtcctgacat ctttgacctg aagttgggcc acctcgttaa
     3001 gtcttgctcc agtgattgca gccagagcgg tgacgtaata atggtatggc ttacctgcgt
     3061 tcttctgaga gtacgcttta gc
//
LOCUS       DADLTF010000264         3049 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.269, whole genome shotgun sequence.
ACCESSION   DADLTF010000264 DADLTF010000000
VERSION     DADLTF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.269"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JN749_004137"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="JN749_004137"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HAY7370317.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JN749_004138"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="JN749_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HAY7370318.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="JN749_004139"
                     /pseudo
     CDS             complement(1963..3049)
                     /gene="bcsE"
                     /locus_tag="JN749_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
BASE COUNT          763 a          786 c          805 g          695 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aagcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgccctta
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctca
//
LOCUS       DADLTF010000265         3047 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.270, whole genome shotgun sequence.
ACCESSION   DADLTF010000265 DADLTF010000000
VERSION     DADLTF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.270"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..461)
                     /locus_tag="JN749_004140"
     CDS             complement(<1..461)
                     /locus_tag="JN749_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAY7370319.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHV"
     gene            complement(508..1227)
                     /gene="yehT"
                     /locus_tag="JN749_004141"
     CDS             complement(508..1227)
                     /gene="yehT"
                     /locus_tag="JN749_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HAY7370320.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1224..2909)
                     /gene="btsS"
                     /locus_tag="JN749_004142"
     CDS             complement(1224..2909)
                     /gene="btsS"
                     /locus_tag="JN749_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HAY7370321.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          787 a          811 c          700 g          749 t
ORIGIN      
        1 ttcacatgct ggcgcgctgc cagtccacgc agaaaataac gtaaaaattg atcgccgcat
       61 tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg taatttccgg catcgaaacg
      121 cggaactgct gttcggtgag gatagccaga atgtcatcgg ttttcagcga aaacgcgatg
      181 cgtaattttt tcagcacgat gttgttatta atgcgacgtt ccggctccag tgccggagca
      241 gactcatcct tgccgcgttt ttcataaatc aggccattga ggaatgacga caaaacaatg
      301 tccggacaac gctgaaaacc ctcttcgtct tctttacgta gccagacggc gatctgttcc
      361 gcggtggctt cgacattatc cagcgccaga atacgcacca ggtcattatt attggctttc
      421 aaaatgtagc gcacgctgcg cagaatatcg ttacttatca tgaggccttc aggtgttgat
      481 gaggcaaaaa gccattttag cagtctttta caggccaatc gcctctttta agcttttcag
      541 ataacggcgg ctgaccggca cggttaagcc attacgcaaa atcaactcgg cctggccgtt
      601 atcttccaga cgaatctcct gtaaatgcgc gaggttaacc agatactgac gatggcagcg
      661 cagtagtggt gtacgacttt ccagggtacg taatgtcaat tcggtaaagc cctctttccc
      721 ttcgtggctg gtaacgtaga caccgctcat ccgactgctg acaaatgcca catctttcat
      781 ttgcagcaaa taaatccgac tatgccccgt acaagggata aatttcagcg cctgttgatt
      841 ttccggtaac agcgaaacat cctgcttgct gcgctcctga cgcaatcgcg ccagcgtttt
      901 ctccagtcgc gcttcatcaa ttggcttcag cagataatca aaggcatgtt cttcaaaggc
      961 tttaatagcg tattcgtcaa acgcagtgag aaaaacaata tacgggcgat gttccgggtc
     1021 aagcatcccc accatttcca gaccactgat gcgcggcatc tggatatcga gaaacagcac
     1081 atccgggcgc agtttatgca ccgcgccgat cccttccacg gcgtttgaac actctccaac
     1141 gatttcaata tcgctctgct cctgcaaaaa tatacgcagg ttctcccgtg ctaacggttc
     1201 atcatcgaca attaagactt taatcatgcc tcgtccctcc atggtagtcg taacgttatt
     1261 cgggtgtaac tatcaggctc acaggcgacg cttattccat agtcatcgcc aaaccgttca
     1321 cgtaaacgct tatccaccag attcatcccc agcccactgg cattggttac cggttgatac
     1381 aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt
     1441 gccactcgcc ctgtatccag cagttgtgat gtcccatgtt taatggcgtt ttccactatc
     1501 ggttgcaggg taaacgcggg caattgctgc tgggataatt cttgcggaat agcaatgttg
     1561 acctgcaacc gcgactggaa gcgcgccttt tcaatttgca gataagcgtt cacatgttca
     1621 atttcgtcgg cgagagtaac aaactccgaa ggccgcttta agtttttgcg gaaaaaagtg
     1681 gaaagatact gcaccagctg gctggcctgt tcgctgtcgc ggcggatcac cgctttaatg
     1741 gtgttaagcg cattaaacaa aaaatgagga ttcacctggg cgtgaagcag tttgatttct
     1801 gactgggtga gcatcgcttt ttgccgctca tattgcccgg caagaatctg cgccgaaagc
     1861 agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac tgaataaacg gtttttggct
     1921 tcatacaatt tgatggtgcc catcacgcgc tgattttcgc cacgcaacgg aattaccaga
     1981 gtcgacccca gtttgcattg cggatgcaaa gagcaacgat aaggtacttc gttgccatca
     2041 gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt aagtcgaaga aatcggtttg
     2101 ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca gcaatttctc tcgatcggta
     2161 atcgcgactg caccaatatc cagctcctga tacagcacct gagccacttt catgctgttc
     2221 acttcgttaa acccctgtcg caaaatgcct tccgtcgagg ctgccacttt cagcgcagtg
     2281 gcagaaaaag ccgaagtgta tttttcaaac atcgcgcgtt tatcgagcaa tatacgcata
     2341 aacagcgccg cgccgacggt attggtgacc atcattggcg cagcaatatt actcaccaga
     2401 cgcaccgcat cttcataagg tcgggcgatc gcaaggatga tcagcatttg caccatttca
     2461 gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga ctttatcagt gcgcccgcga
     2521 cggatcagga tgctgtgtac caggccgccg agcaatcctt caacgatggt tgagatcatg
     2581 caacttagcg cggtcatgcc ccccatcgaa tatcgatgta agccgccggt cagaccaacc
     2641 agcccaccga cgaccggacc gccgagtaag ccgcccatta ccgcgcctat cgcacgggta
     2701 ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc ccatgatgca gaagatggaa
     2761 aagacgatgt agcagagaaa tttatgcggc agacgaaccg tgacctgcat taacggtatg
     2821 aataatggcg ttttactcat taaccacgca atgactaaaa aaacgcacat ctgctgaagc
     2881 agcagcaaca ccagattaaa atcgtacata cccgcaaacc acacttccct ttaaaacgcg
     2941 taacatacat tgcctgcgtt taactttctt tgaactcttg cagaaaaatg agaattcgtg
     3001 agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac cctatag
//
LOCUS       DADLTF010000266         3037 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.271, whole genome shotgun sequence.
ACCESSION   DADLTF010000266 DADLTF010000000
VERSION     DADLTF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.271"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            52..1083
                     /gene="ynaI"
                     /locus_tag="JN749_004143"
     CDS             52..1083
                     /gene="ynaI"
                     /locus_tag="JN749_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HAY7370322.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1134..2747)
                     /locus_tag="JN749_004144"
     CDS             complement(1134..2747)
                     /locus_tag="JN749_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAY7370323.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          791 a          705 c          671 g          870 t
ORIGIN      
        1 gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct
       61 gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg
      121 attctgatga gcttttggtt tcgccgtgga aatcgtaaaa gaaaaggatt tttattccat
      181 gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat cattaattat
      241 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
      301 tccctgattg cggttattct gacgattaag ctatttctgc tgattaatca gtttgaaaaa
      361 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
      421 atcaccatta ttgtagtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
      481 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
      541 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
      601 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
      661 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
      721 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
      781 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
      841 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgctgac
      901 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgagtggctt
      961 gctgcacagc aagacgttta tttgaagatt atcgatattg tacagtcaca cggcgcggac
     1021 tttgccttcc ccagccagac gctgtatatg gataacatta caccgccgga acagggtcgc
     1081 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
     1141 ttcacaatat acatagtccg actgtacgct acatcttcag ggttattaat gggataacct
     1201 ttcagccacg gtttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
     1261 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
     1321 tccgtcgatg cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt
     1381 cctgaatgca ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
     1441 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
     1501 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
     1561 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
     1621 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
     1681 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtataaaa
     1741 tgccacgctg gcttttcgcc cgtccccaac actttttcgg tcatcaggcg gcgatctatc
     1801 gtcatactta atgccaggcg aacgcgctga tctgccgtcg gccctttttg cgtgttaaag
     1861 gcataataat aggtcccgag ctgcggtggc gtataaacct gccccggaat atccttcaac
     1921 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
     1981 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagcaccgtt
     2041 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgc
     2101 tctttaagaa cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg
     2161 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
     2221 cacggcaacg gtttatcaag ctgaattttc aaagtatggg catcaactgc ggtgacgcca
     2281 agctgatcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
     2341 gcggcaaacc atgcaaatgg cgacaatgtt tttgggtcca ccagacgttg ccagctgtag
     2401 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
     2461 aaagtccaga tacggttgtc attacttttc cattgagtcg cgacgccggg gacaatctcc
     2521 cctttttcgt tctgattcac cagaccttca aacagatcgc gaatgacctg aatctctggc
     2581 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
     2641 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
     2701 ctgctgacca acagcgcaca acacgtaact gaaacagagt gcttcataag tttccctcta
     2761 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
     2821 catcaaaaaa gtgacaaagg aagcatcaaa tgttttaagt atcaatatat caaatcagat
     2881 ttagtgtaaa taatagacgc caaaatgcgg caaagcacat agaacaccga acactacgtt
     2941 cgggcttcgc acgttcaaac cagttttcgg tatcataatc agcatctgtc gccattgtac
     3001 gaagaataaa aaataaccaa aacgttgaaa ggaactg
//
LOCUS       DADLTF010000267         2963 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.272, whole genome shotgun sequence.
ACCESSION   DADLTF010000267 DADLTF010000000
VERSION     DADLTF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.272"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            143..2011
                     /gene="kup"
                     /locus_tag="JN749_004145"
     CDS             143..2011
                     /gene="kup"
                     /locus_tag="JN749_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HAY7370324.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2178..2597
                     /gene="rbsD"
                     /locus_tag="JN749_004146"
     CDS             2178..2597
                     /gene="rbsD"
                     /locus_tag="JN749_004146"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAY7370325.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2605..>2963
                     /locus_tag="JN749_004147"
     CDS             2605..>2963
                     /locus_tag="JN749_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAY7370326.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          660 a          703 c          782 g          818 t
ORIGIN      
        1 aagggggggg gctttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt
       61 gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca
      121 tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac
      181 cctcgcggcg attggagttg tctacggcga tattggtacc agcccgttat atacacttcg
      241 tgaatgtttg tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt
      301 atcgctgatc ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat
      361 gcgggcagat aacgccggtg agggggggat cctgacgttg atgtcgcttg ccgggcgtaa
      421 tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt
      481 ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga
      541 aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt
      601 attatttatg attcaaaagc atggcacggc tatggtcggt aagctgtttg cgccgatcat
      661 gctgacctgg tttttgattc tggcaggact ggggttacgt agcattattg ctaacccgga
      721 agtgctgcat gcgctgaatc cgatgtgggc ggtgcatttc ttccttgaat acaaaacggt
      781 ttcttttatt gcattagggg cagtggtgct gtcgattacg ggggtcgagg cgctgtatgc
      841 tgatatgggg cactttggta agttccctat tcgcctggcg tggttcaccg tcgtattacc
      901 ttccttgacc cttaattact tcggtcaggg agcgttgttg ttaaagaacc cggaagcgat
      961 taagaacccg ttcttcctgt tggcaccgga ctgggcgctg atcccactgc tgatcatcgc
     1021 cgcactggcg acggtaattg cctcgcaggc ggttatctct ggcgtcttct cattgacgcg
     1081 tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga
     1141 gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt
     1201 gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg
     1261 aaccatggta ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa
     1321 taagtatttt gttgcgctga tcctgattgc tttcctttgt gtcgatattc cattgttcac
     1381 cgctaacctc gataaactgc tctccggcgg ctggttgcca ttgagcctcg gtactgtgat
     1441 gtttatcgtg atgaccacct ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga
     1501 acatggtaac tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt
     1561 gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca
     1621 taaccttaaa cataacaagg tattgcatga gcgggtgatt ttgttaactc tgcgcaccga
     1681 agacgcgcca tatgtccata acgtccgtcg ggtacagatt gaacaactgt cgcccacttt
     1741 ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca
     1801 ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca
     1861 tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt
     1921 gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc caaacagggt
     1981 tatcgaactg ggtactcagg tcgaaatcta acgccagacg cctcctttct tcataagggg
     2041 gcgtttttgt tttcatggtt aatcaccatg taaaacgttt cgaggttgat cacatttccg
     2101 taacgtcacg atggttttcc caactcagtc aggattaagc tgcggatcag cgaaacgttt
     2161 cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga
     2221 tctcccgtct gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca
     2281 aaagtacaac gcgtatcgat atggcattaa cccaaggtgt accttctttt atgcaggtgc
     2341 tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac
     2401 aacataatcc gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc
     2461 agggaaatac cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag
     2521 aaagtcaggc ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg
     2581 ctggcgtgac gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa
     2641 gccttcccgg gtgtaaaagc cctctcgggc gcagcgttaa atgtctatcc gggccgtgtg
     2701 atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggt
     2761 atctatactc gcgatgccgg cacgctttta tggctgggga aagaaacgac atttaccggg
     2821 ccgaaatctt cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg
     2881 cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa
     2941 attgactgga aaaccatgta tgt
//
LOCUS       DADLTF010000268         2923 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.273, whole genome shotgun sequence.
ACCESSION   DADLTF010000268 DADLTF010000000
VERSION     DADLTF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.273"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..546)
                     /locus_tag="JN749_004148"
     CDS             complement(<1..546)
                     /locus_tag="JN749_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370327.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AVTDKSYLSLNPDKYPNYRFLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..2194)
                     /locus_tag="JN749_004149"
     CDS             complement(1208..2194)
                     /locus_tag="JN749_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370328.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTRGTLQQDALNICDNVVRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGADRGVFMMAKRFTALSFGCFRHDGENITV
                     VLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVLPVVKRLSFTKTS"
     gene            complement(2276..2767)
                     /locus_tag="JN749_004150"
     CDS             complement(2276..2767)
                     /locus_tag="JN749_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370329.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRVEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
BASE COUNT          766 a          682 c          721 g          754 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaaaacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgtta ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagattg atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgtaccc atgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga cgtcagtccc cagcatatcg acgatagcgt
     1501 cttcaccacc gaagccctgc ttagcagcca gacgggagag agccttgagg tgtgactcat
     1561 gggcgataat gccgtagtca gaaacctcag aaccgaaaga agactccgtg ttctcgtaca
     1621 gggcatccat gatagcgtca gccttagcgg tcaggctacc agtcagggcc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttagagtta atggtacgga
     1741 ccacgttatc gcagatgttc agagcatcct gctggagcgt cccccgagtg aaaccagtag
     1801 aggacttctg gtcccagagc tttgtctgat gagacacgag gatagcctgt tcaatcggct
     1861 caacggtgat ttcagtacca gacggagcgg tcacgacttc aggtcgctta ccaccacgga
     1921 caggaacgac cacagagcgg tcagccagag tgtgagcaat gattggcgtc ggtaagctgt
     1981 tacgggtcgg caggacttct acagggaacg gaatgttttc ctgagcgtct ttaagtacac
     2041 cgttcagcat agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt
     2101 tagtaggcag accttcattg acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa
     2161 taccgaactg gaaagcgtct ttaatcgtca taataatcaa tctcctaaat tagtttaaat
     2221 actgtgagtc ctcaaaactg aggagtccct agcatcgggt cagggactgt gtgttttaca
     2281 ggctgtccat ccagttaccg cctgactcaa ggtcagcgac gatagcggca cgggagcggc
     2341 cagcggaatg ggtagcactg cggaggtttt caccataggt ccggcgttca gctttgacct
     2401 gttcgcggcg ctgtagctct ttctgccatt cgtcttcggt catgaacatt tcgcgggtac
     2461 gctctttctg agacgccagc tctttagcga tacgctcagc ctcgacacgc ttattcatct
     2521 ggcggcgttg gtaggcggtc atcaggtcat tactgaagaa gttaccaatg gtcacgccag
     2581 cttcagccag agcttccagt aactccattg cctcgcggct ggtagacggc aggcctgaca
     2641 ggtagcccgg aatgagggtc ttgatagcga gcgacagcgg atagggctga ccgtccagac
     2701 tcacagtatt ctcgtagaga ttgatgtcgg tgactgacag tttccagtct tggttattga
     2761 ataacatagt aattactcct cttgagagtt gagactatcg gtcgaccaca ggcgtgttca
     2821 gtcctgcggc gtctccccgc atcggtcatc tcaaaaggtt taaaattagg tattgagtag
     2881 acgcgctact aacggtgtgc tgacgacagc tagcaagcta gcg
//
LOCUS       DADLTF010000269         2870 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.274, whole genome shotgun sequence.
ACCESSION   DADLTF010000269 DADLTF010000000
VERSION     DADLTF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.274"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="JN749_004151"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="JN749_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HAY7370330.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="JN749_004152"
     tRNA            complement(621..695)
                     /locus_tag="JN749_004152"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(771..1703)
                     /locus_tag="JN749_004153"
     CDS             complement(771..1703)
                     /locus_tag="JN749_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HAY7370331.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMVIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1997..2752
                     /gene="mlaA"
                     /locus_tag="JN749_004154"
     CDS             1997..2752
                     /gene="mlaA"
                     /locus_tag="JN749_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HAY7370332.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPVRNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          695 a          697 c          689 g          789 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcatgagta tacccgctaa
      721 tggactgcgg ggtaagtacg ctgccgctcg attgcttaaa ccctcgccat ttatgctggg
      781 tttttatcat ttttcttaat gttttccgca cgttctgctt tttggcgtgc ttctgcttta
      841 cgcttgttgc tcatgtcgtt acgaatctgt gcatgactca ttaacgcgaa gataaaggtg
      901 ccgccgcaga tgttccccgc taaagtaggt agtgcgaagg gccagatgaa atcgctccag
      961 tgcagcgtac cgttaaacac cagatagagg atttcaacag aaccgaccac gatatgggtg
     1021 gtgtcaccca gggcaataag ccaggtcatc aatataatca ccacaatctt tgccgcacct
     1081 gctgcaggaa acatccatac catagtggcg atcagccagc cggaaatgat cgcgttggca
     1141 aacatctcgc tgggggtgtt cttcatcaca tccatgccga ttttgacaaa tgcatcgcga
     1201 gtttcttcat tgaagatagg catatattca aatgcccacg cagcaatacc agtcccgaga
     1261 atattaccca gcagcacgac tccccataac cgcataagta agccaacgtt gctcattgtc
     1321 ggtttttgca tgacgggtag taccgcagtc acggtgtttt cggtaaataa ttgctggcgg
     1381 gccattatga cgataataaa accaaaggta taaccgagat tctccagcaa gaagctgccc
     1441 ggcacacctt ccagttcgac atgaaatatc ccttttgcca gtaacgaagc gcccatcgac
     1501 agacccgccg caatggctga ccacagtagc gccattgcgt cgcgttctag ctctttttca
     1561 ccatcctggc ggatatgctc atgaattacc atcgctcggg aggggagtcg gtcttcatct
     1621 atttctattt ttttgccgcg ctctttttct tcgctctcaa cttcaatttc gtcgctgtgt
     1681 tgatcaattt tgtcgttgtc catggtctct tctcttgaat tagcacgtat agctaaagcg
     1741 tagcggcttt tttgctcgca actggcggga gttactctga aaatgtagaa aaggttgcgt
     1801 ttgccttttt ctgtttctat agaatcaagt agcctacagg gcggcgatta ccaggctatg
     1861 atcaaatcag caaatcaggg cgtctggaca tcagttgacg tgctgttaca atcgcccaca
     1921 cctaaacagg cggatacggt atcgttccgt catggatggc aaactgcata agccataaaa
     1981 aaacagggag acatttatga agcttcgcct gtcggcgctt gctctgggaa ctacgcttct
     2041 ggtggggtgt gcgagttccg gtacagatca gcaagggcgt tctgacccgt tagaagggtt
     2101 caaccgcacc atgtacaact tcaacttcaa cgtattagac ccgtatattg ttcgaccggt
     2161 cgctgtcgcc tggcgtgatt atgttccgca accggtgcgt aatggtttga gcaactttac
     2221 tggcaacctt gaagaacctg cggtgatggt taactacttc ttgcagggcg acccttatca
     2281 ggggatggtc cactttaccc gctttttcct gaacaccatt ttggggatgg gcggctttat
     2341 tgatgttgca gggatggcga acccgaaact gcaacggact gaacctcacc gcttcggtag
     2401 tacgcttggt cattatggcg tgggttatgg gccttacgtt cagttaccgt tctacggtag
     2461 cttcacgctg cgtgatgacg gtggtgatat ggcggatggt ctttacccgg ttctttcctg
     2521 gctgacctgg ccgatgtctg tgggtaaatg gacgcttgaa gggatcgaaa cccgcgctca
     2581 gctgctggat tccgatggtc tgctgcgtca gtcgtccgat ccttatatta tggtgcgcga
     2641 agcgtacttc cagcgtcatg atttcatcgc taatggcggc gaactcaaac cgcaggaaaa
     2701 tccgaacgcg caagcgattc aggatgattt aaaagatatc gattctgaat aagaaacaaa
     2761 taaaaaaggt gagtcgcaaa actcaccttt tttgttgtta tccctcaatt accacaagta
     2821 aaaggagata aaccgacaat gacccggacg taatgatgta aaaatactga
//
LOCUS       DADLTF010000270         2834 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.275, whole genome shotgun sequence.
ACCESSION   DADLTF010000270 DADLTF010000000
VERSION     DADLTF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.275"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..420
                     /locus_tag="JN749_004155"
                     /pseudo
     CDS             1..420
                     /locus_tag="JN749_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847259.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1004..1720
                     /locus_tag="JN749_004156"
     CDS             1004..1720
                     /locus_tag="JN749_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370333.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSYKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2272..2676
                     /locus_tag="JN749_004157"
     CDS             2272..2676
                     /locus_tag="JN749_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370334.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
BASE COUNT          817 a          544 c          627 g          846 t
ORIGIN      
        1 catgatcggg gaagacccag atatgaacag gcacattcag agttacttta aagagcttac
       61 tgaacgtctg aggtgttaga gccatgactg ccgcagcatt gctggcggaa atgcctgaat
      121 cgagcagcct ctcgagacgt gaaatcagcg cacttgtcgg tgttgctcag gtaaaccggg
      181 actcgggaac tctgcgcggt cgacgaacca ttttcggtga ttgtgctggg gaagaacagc
      241 tctgtacatg gcggcgcttg aggcccccgg tttaatctgg tgataaaggc gttttacatg
      301 cgcctacttg cagccggaaa cgccaaaaaa gtggcactgg ttgcctgtat gcgtaagttg
      361 ctgaccatta tgaacgcaat gctcagaaaa aacgaagaat ggaatgaatc gtatttatga
      421 aaactggatt gtgggccata aatattccaa aaagatagcc cgctccgttt attaccggaa
      481 cgggcgagag catgacattg tctgttatta taatgagact gctgattcta cttattttta
      541 atttcaagaa aagcgttttc tacaaagaat tatgagattt aatgttttgt ttagacaata
      601 ttatcattct gttcaataat gaaagtcttc atcaacaaca agtaaaaaca acgtccctct
      661 catcgaaccg gacttgcaga tttcccgcat ccggctctct tgcaacacat cagaccttca
      721 cgttcgggcg gttatgggga aggtccagca agctgtatga gccctaactg tttgaatacc
      781 agtagattac cagaaacttc tatcgattga agacattacc aactagcgtt actaaatgtt
      841 gaccaagttt tgttcacttg tctggttttg atagtagcat tctgcagagt attttgatat
      901 gataaatatg cattaacaat gatattattg ttaatttcct agcataaaaa ttaaaagatc
      961 aacttttatc tcgaatattt tttacattat cagttggttt cttatgaatg acaattcatt
     1021 gcttagaaat tcttcattat tcatcgccta tatgggatgt gtagggtggg tcagtgctta
     1081 ttcttatggc tggggaacct ctttttacta tggatttcca tggtgggttg ttggtgcagg
     1141 tttggatgat gttgccagaa gtttactcta tgctatcata gtaatgggta tactttttac
     1201 cggttggggg attggtgttt tattttttct tcttataaag aaaagaagta aaatacaaga
     1261 cttgagtttt tttagactat tttttgctat taccctactg tttttccctg taatattcga
     1321 acttttaatt ctcaaacaat atttcatact tccattatca ctttcatgca ttattagttc
     1381 attggttatt tcaataataa ttcgtatata tggacggatc ttttctgtat catgtttttc
     1441 agatatccct tttgttagaa aacaccgcat taagttaatt atggctggtt ttttggttta
     1501 tttttggctt ttttcctttc ttgttggctg gtataaacct caattaaaaa aagaatatca
     1561 gatgctttgt tataataata gttggtatta tgttcttgct cgttatgatt cacggctcgt
     1621 tttgtctagt tcatataaag atgacagtaa cagattctta atatttaata cagaacagtc
     1681 gggattttat gaaatcaatg atgtttatgt cagaaagtaa taatcctctc ccacaaagtg
     1741 aattaaatga atatataatg cgttcgaagt atatgtattg agctgtcata ctgagaaaca
     1801 aaccttaggg cagcacaatt aaagccagaa acaactatgc aagaaagtta ctaaataagt
     1861 gctcggaaca gtaaaacact caggggggac tcagtccgga ttatgtaatc agataaattg
     1921 ccaaacatca caaatcacca gccggactga gtgatgccga gcattgcacc aataccccgt
     1981 gatgaacgat gcctgatgca gaaagctccc tataaaacaa agaacaaaaa ctacgaccgg
     2041 cgattcaccg tcatactgat gctgcatcgt ggtgacactg tctgtcactt tcatgttgac
     2101 tgaacaactc tgctgcgaag aaggtgtacc gccggcggga tttgattgtc cggcgtctag
     2161 cttccacttc cctgatggaa ctgaaagttg ttatggccgg tcagcttgcc ctgtccgcag
     2221 gacatgaggt gctgtgacgg acagcaccag taacggaaag tgacaatgaa aatgaatact
     2281 gcgctgatag ttgcactaat gtgtatgtgg tatgcggtac ccgctgctgc aaaagagact
     2341 ttactggcta tgccacgtaa ttcaacagaa cattgctatg cggagattaa tgtgcacgga
     2401 ccatatgggg tgtatttccg agtggtcccg catccccccg ggggaaagag ctgggtcgaa
     2461 tgtaacagtg actactatta ctctgataaa ccaccgggag tacagatact cggaacccgt
     2521 gcagggtgtc gggtatacgg aatctgcggg accacctcga cactgcatgt ggctggaaga
     2581 ggggttgtgt gtattaaaaa tatatgcagt ccacgtggga tgataattca caggatacgg
     2641 aaacgtcctg tagtggctgt aagcgatgag atgtgatctt acccagcaat agtggacacg
     2701 cggctaagtg agtaaactct cagtcagagg tgactcacat gacaaaaaca gtatcaacca
     2761 gcaaaaaaac acgtaaacag cattcgcctg aattttgcag tgaagccctg aagcttgctg
     2821 aacgcatcgg tgtt
//
LOCUS       DADLTF010000271         2829 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.276, whole genome shotgun sequence.
ACCESSION   DADLTF010000271 DADLTF010000000
VERSION     DADLTF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.276"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1068)
                     /locus_tag="JN749_004158"
     CDS             complement(<1..1068)
                     /locus_tag="JN749_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAY7370335.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRA"
     gene            complement(1647..2324)
                     /gene="bioD"
                     /locus_tag="JN749_004159"
     CDS             complement(1647..2324)
                     /gene="bioD"
                     /locus_tag="JN749_004159"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HAY7370336.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(2317..>2829)
                     /gene="bioC"
                     /locus_tag="JN749_004160"
     CDS             complement(2317..>2829)
                     /gene="bioC"
                     /locus_tag="JN749_004160"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HAY7370337.1"
                     /translation="HKDAADHYLAGDIESLPLATATFDLAWSNLAVQWCGNLSTALRE
                     LYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERPHANRFLPPDEIEESLNGVRYQHH
                     IQLITLWFDDALSAMRSLKGIGATHLHEGRDPRILTRSQLQRLQLAWPQQQGRYPLTY
                     HLFLGVIARE"
BASE COUNT          681 a          763 c          696 g          689 t
ORIGIN      
        1 cgcccggtca ccgcgataca tgccgccaat ttgtggaatg gtgacgtgag attcatcgac
       61 gaccagcagc ccatcggcag gcaggtaatc aaacagcgtc ggcggtggct cacccggtcc
      121 acgaccggag aggaagcgcg agtagttttc aatccccgaa cagtagccca gctcgttcat
      181 catctccaga tcaaactggg tacgctgggt cagccgctgc tcttccagca gtttgttgtt
      241 ttccaacagc actttgcgtc tggcggccag ttcttctttg atctcctcca tcgcctgtac
      301 gatgcgctcg cgcggtgtga cgtagtgcgt tttcgggtag atggtaaaac gtggaatagt
      361 ggaaacaatc tgcccggtca gcgggtcaaa taacgacaat cgttccactt cctcgtcaaa
      421 cagttccacg cgaagtgcaa tgtcatccga ttctgccggg aagatatcta tcacctcgcc
      481 acgaacgcgg aaagtaccac gctggaatgc ttgatcatta cgagcgtatt gcagctccgc
      541 cagtcggcgc agaatcgcgc gctgatcgat aatcataccg accgtgagat ggagcatcat
      601 cttgagatat aaatcaggat cgcccagacc ataaatcgcg gaaacagacg ccactacaat
      661 cacatcacgc cgctccagca tcgctttggt ggcggacaaa cgcatctgct caatatgttc
      721 gttaaccgag gcatctttct caatgaaggt gtcggaactc ggtacatagg cttccggctg
      781 atagtagtcg tagtaggaga cgaaatactc caccgcgttt tccgggaaga actctttcat
      841 ttcgccatac agctgggccg ccagcgtttt gttgggcgca agtaccatgg ttgggcgctg
      901 aaggtcagca atgacattgg caatggtgaa ggttttcccg gagccagtca cgccaagtaa
      961 cgtctggtgc gccaggccat cttccagccc ctcttcgaga cgtcgaatcg cctctggctg
     1021 atcgccagaa ggtttaaaag cggaattcag tttgaacggt ttactcatga gtcgctacct
     1081 gaaggagttg ggcgggcagg tatgtaattt tactcgtcgt acttaattat gccaacaaat
     1141 tatactggat aaaaaaacag ttcatcacca taatatttct gatacagcgt aaactccgct
     1201 gtcaatcatg agcaaaattt actctgtggc gagataaaac tccggcctta ccgggttatc
     1261 cccaaagcaa cggctttttt aacatttgtc aagatgagtg atgacagttt tatggcaaga
     1321 gatgcctgta cagtgactgc cattgcattt atctaaccag ttaaaaaata aaagatattt
     1381 ctttgagccg tctttaacgc caattcgcgt gcaagccgcg tattctctcg cttgcctcgt
     1441 gttttctaac tctaatacac atggttatcc acaggaatag tggataactg tctccagccc
     1501 ctatcccccg ccgcctgggc aattcccaca ttcgccgcat gaggcggaag taaaattttt
     1561 ttcgtgatta ttttagaatt taattggtta aattgcagtc aatcgaagac gcaatctcgc
     1621 tcgcaattta accaaataca gaatggctac aacaaggcaa ggtttatgta ctttccggtt
     1681 gccgcatttt ctggattttc tgcaagccag gggatctctc ccagcagcgg cgcgggaatc
     1741 atgcgggtga gcgtggtcat atattcagcg tgacgttttc ccggaggcgt aacatcgttc
     1801 gccacccaac ccgccagagt cagtccggcg tgttgtatta cctgcgcagt caacatcgcg
     1861 tgattaatac agccgagttt cacaccaact accagtatca ccggcagttg ttcctgtgtt
     1921 acccaatctg caaaagtgaa agtgtcagaa agcggcgtaa accagccgcc agcaccttcc
     1981 actaacaccc agtcagcctg ttgttcaagc gcgcgtaatc cggcgctcat taccaatgat
     2041 tctatcggcc tgccctcttg ggcgctgatg atgtgcggcg aggtgggttc tgcgaaggtg
     2101 taaggattta ccgttgcgta atccagctgc aggctgctgt tgtgctgtaa cgccagcgcg
     2161 tcactattac gtaagccttc cggggtcttt tcgctgccag aggcgaccgg tttataacct
     2221 gccgtccgat aacctactgc cttcgcggct tgtaaaagtg cacaactggc gacggttttc
     2281 cccacttcgg tatccgttcc ggtgacaaaa taacgtttac tcacgagcaa tcactcccaa
     2341 aaaaagatga tacgtcagag gatatcgccc ctgttgttgc ggccaggcca gttgcaatcg
     2401 ctgcaactgc gaacgcgtta atattcgcgg gtcgcgccct tcatgaagat gcgtagcacc
     2461 gatgcctttc agcgaacgca tggcactgag cgcatcatca aaccatagtg tgatgagctg
     2521 aatatgatgt tgataacgca cgccgttcag cgactcttcg atttcatctg gcggtaaaaa
     2581 gcgattagca tgcggacgct cgtccaccgc ctgccgcgcc tgatgcagtt cgggtaacga
     2641 tccctgcacc agcgtggtaa acgcgaccac gcctccgggg cgcaccaccc gatacagctc
     2701 gcggagtgcc gtggataaat taccgcacca ctgcaccgcg agattgctcc atgcaagatc
     2761 gaacgtcgca gtcgctaacg gcagggattc gatatctccc gccagataat gatctgcggc
     2821 atctttatg
//
LOCUS       DADLTF010000272         2795 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.277, whole genome shotgun sequence.
ACCESSION   DADLTF010000272 DADLTF010000000
VERSION     DADLTF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.277"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..388
                     /locus_tag="JN749_004161"
     CDS             <1..388
                     /locus_tag="JN749_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="required for the translocation of capsular
                     polysaccharide through the outer membrane; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HAY7370338.1"
                     /translation="NDDLKVFVMGEVGKQSTLKMDRSGMTLAEALGNAEGMNQDVADA
                     TGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQLEPYDIVYVTTAPLARWNRV
                     ISLLVPTISGVHDLTETSRWIQTWPN"
     gene            376..822
                     /gene="etp"
                     /locus_tag="JN749_004162"
                     /gene_synonym="yccY"
     CDS             376..822
                     /gene="etp"
                     /locus_tag="JN749_004162"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HAY7370339.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            842..>2795
                     /gene="etk"
                     /locus_tag="JN749_004163"
     CDS             842..>2795
                     /gene="etk"
                     /locus_tag="JN749_004163"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HAY7370340.1"
                     /translation="MTTKNMNTPLGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMLPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINTLQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQHQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVSAVLARAMLR
                     RGVEAPEQLEEHGISVYSTIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPM"
BASE COUNT          739 a          661 c          745 g          650 t
ORIGIN      
        1 caacgatgat ctgaaagtct tcgtgatggg tgaagtgggt aaacagagca cgctgaaaat
       61 ggaccgcagc ggtatgaccc tggcagaagc gctgggtaac gctgagggta tgaatcagga
      121 tgtcgctgat gcgacgggga tttttgttat tcgtgcaacg caaaacaagc agaatggcaa
      181 aatcgccaat atttatcagc tgaatgcgaa ggacgcatcg gcaatgatac tcggaacgga
      241 attccagctt gaaccttacg acattgttta tgtcactacc gcgccgctgg ctcgttggaa
      301 ccgtgtgatt tctctgctgg taccgacgat ttccggggtg catgacctga cagagacttc
      361 acggtggatt caaacatggc ccaactaaaa tttaactcaa tcctagtggt ttgtaccggc
      421 aacatttgcc gttcgcccat tggcgagcgc ttgctgcgta aacgtctgcc gggcgtaaaa
      481 gtcaaatcgg caggtgttca tggtctggta aaacaccctg cagatgcgac agcggcagat
      541 gttgccgcta atcatggcgt atctcttgaa ggacatgctg gacgtaagct cactgctgag
      601 atggcacgaa attacgatct gattctggca atggagtcgg aacatattgc tcaggttacc
      661 gcaattgctc ccgaggtccg cgggaagaca atgctgtttg ggcaatggct ggaacagaaa
      721 gagatcccgg atccctatcg taaaagtcag gacgcatttg aacatgtcta cggtatgttg
      781 gagcgcgcca gtcaggaatg ggcgaagcgc ctcagccggt aatttgagtt tataaaatac
      841 gatgacaact aaaaatatga atacgccact aggcagcact caggaaaatg agatcgatct
      901 gcttcgtctg gtcggcgagt tatgggatca ccgtaagttt attatcagcg tgaccgcgtt
      961 attcacgctg atcgctgtcg cttactcgct gttaagcaca ccaatttatc aggcagatac
     1021 tctggtccag gttgagcaaa aacagggcaa cgccattctc agcggcctga gcgatatgct
     1081 ccctaactca tcgcccgagt ctgcaccgga gatccaactg ctgcaatcgc gcatgattct
     1141 cggtaaaacc attgctgaac tgaatctgcg cgacatagtt gagcagaagt attttccgat
     1201 tgtgggtcgc ggctgggcga gattaaccaa agaaaaacca ggtgagctgg cgatcagctg
     1261 gatgcatatt ccacaactga atggtcagga tcagcaactg acactcacgg ttggggaaaa
     1321 cggccactat acactggaag gtgaagagtt caccgtcaat ggtatggtcg gccagcgtct
     1381 ggaaaaagat ggcgttgcgc tgactatcgc ggacattaag gccaaaccag gaacacagtt
     1441 tgtcctgagc cagcgtaccg aactggaagc gattaacaca ttgcaggaaa cctttaccgt
     1501 tagcgaacgc agtaaagaaa gcgggatgct ggaacttacc atgactggtg atgatcccca
     1561 gttgattact cgtattctga acagcatcgc taacaactat ttgcaacaga atatcgctcg
     1621 ccaggcggcg caggattcac aaagccttga attcttacag caccagttac ctgaagtgcg
     1681 cagcgagctg gaccaggcgg aagaaaaact caacgtttat cgccagcagc gcgattcggt
     1741 tgaccttaac ctggaagcca aagccgttct tgagcagatt gtgaacgttg ataatcaact
     1801 caatgagctg accttccgcg aggcagagat ctcccagctg tataagaaag atcacccaac
     1861 ttatcgtgcg ctgctggaaa aacgccagac gctggagcaa gaacgcaaac gcctgaataa
     1921 gcgggtatcg gcaatgcctt ccacccaaca ggaagtgttg cgtttaagtc gtgacgtaga
     1981 agcgggccgt gcggtatatc tgcaattact taaccgccag caggagttga gtatttcgaa
     2041 atccagtgcc attggtaacg tgcggattat cgacccggca gtcactcagc cgcagccagt
     2101 gaaaccgaaa aaagcgttga atgtggtgct tggttttatt cttggcctgt ttatttcggt
     2161 gagtgccgtg ctggcgcgtg cgatgttgcg tcgtggtgta gaagccccgg aacaactgga
     2221 agagcacggc atcagcgttt attccactat cccaatgtcc gagtggctgg ataaacgcac
     2281 ccgtctgcgt aagaaaaatt tattttctaa tcagcagcgc catcgtacta aaaatatccc
     2341 cttcctggcg gtggataacc cggcggattc tgctgtggaa gccgtacgtg cgctacgaac
     2401 cagtctgcat ttcgctatga tggagactga gaataacatt ctgatgatca ccggtgcgac
     2461 gccagacagt ggtaaaacgt ttgtcagttc aactctggca gcggtgatcg cccagtccga
     2521 tcaaaaagtg ttatttattg atgccgactt acgccgtggt tattcgcata acctgtttac
     2581 cgtgagtaat gaacatggct tgtcggaata tctggcaggt aaagatgagc tcaacaaagt
     2641 gatccagcat tttggcaaag gaggctttga tgtgattact cgcggtcagg tgccacctaa
     2701 cccgtctgaa ctgctgatgc gcgatcggat gcgtcaatta ctggaatggg cgaacgacca
     2761 ttacgatctg gtgattgtcg atacgccgcc gatgc
//
LOCUS       DADLTF010000273         2697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.278, whole genome shotgun sequence.
ACCESSION   DADLTF010000273 DADLTF010000000
VERSION     DADLTF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.278"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            258..1673
                     /gene="gltX"
                     /locus_tag="JN749_004164"
     CDS             258..1673
                     /gene="gltX"
                     /locus_tag="JN749_004164"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAY7370341.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(1725..2096)
                     /locus_tag="JN749_004165"
     CDS             complement(1725..2096)
                     /locus_tag="JN749_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7370342.1"
                     /translation="MTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGGRARLILV
                     DTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTDIEQEKVA
                     QFLSREGIRNFLARLDIDESA"
     gene            complement(2119..2463)
                     /locus_tag="JN749_004166"
     CDS             complement(2119..2463)
                     /locus_tag="JN749_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HAY7370343.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
BASE COUNT          667 a          649 c          673 g          708 t
ORIGIN      
        1 cgctgtgtgg agtcgcatta tagggagagt tcaaaatgag tcaacgcatt ttctaaagaa
       61 attgttcgtt cgtcgtaaat ttaagcaaga tgatcgcaaa acagaccgtg ttgcgcaatt
      121 tgtcaacgaa aacaataatg cgtaaggtag aaacccgaac tacattgagg aatcaggcgg
      181 gagtgataga atatcgccca ctcaattttt ccaggatttg ccggttgtcg gcatctttct
      241 aaacgtaagg ccatttcatg aaaatcaaaa ctcgcttcgc gccaagccca acaggctatc
      301 tgcacgttgg cggcgcgcgt actgctcttt actcctggct ttttgcacgt aaccacggcg
      361 gtgagttcgt gctgcgtatt gaagacaccg atcttgagcg ttccacgccg gaagctatcg
      421 aagccattat ggatggcatg aactggctga gcctggagtg ggatgaaggt ccgtactacc
      481 agaccaaacg ttttgatcgc tacaacgcgg tgatcgatca gatgctggaa gagggcactg
      541 cttataaatg ctattgctct aaagagcgcc tggaagcgct gcgcgaagag caaatggcga
      601 aaggtgagaa gccgcgttat gacggtcgct gccgccacag ccatgagcat catgctgatg
      661 atgaaccgtg tgttgtacgt tttgctaacc cgcaggaagg ttctgttgtt tttgacgatc
      721 agatccgtgg tccgatcgag ttcagcaacc aggaactgga cgatcttatt atccgccgta
      781 ccgatggttc cccaacctat aacttctgtg tggttgtcga tgactgggat atggaaatca
      841 cccacgttat ccgtggcgaa gaccatatca acaacacgcc gcgccagatc aacattctta
      901 aggccctgaa agcgccggtg ccggtttacg cgcacgtttc tatgatcaat ggcgatgacg
      961 gtaaaaaact gtccaaacgt cacggggcag tcagcgtaat gcagtatcgt gatgacggtt
     1021 atttgccaga agcactgctg aactatctgg tgcgtctggg ctggtcccac ggcgatcagg
     1081 aaatcttcac tcgtgaagag atgatcaaat acttcacttt gaatgccgtc agcaaatctg
     1141 ccagtgcgtt caacaccgac aagctgctgt ggctgaacca tcactacatt aacgcgctgc
     1201 cgccggagta tgttgctact cacttacagt ggcacattga gcaggaaaat atcgataccc
     1261 gtaacggccc gcagctggct gatctggtga aactgctggg cgaacgctgc aagacgctga
     1321 aagagatggc acagagctgc cgttatttct acgaagattt tgctgagttc gatgccgatg
     1381 cggcgaaaaa acatctgcgt ccggtagcgc gtcagccgct ggaagtggtt cgtgacaaac
     1441 tggccgcgat tactgactgg accgctgaaa acgttcatca cgctattcag gcgacggcgg
     1501 atgagctgga agtgggtatg ggtaaagttg gtatgccgct gcgtgtcgcc gtaaccggtg
     1561 cggggcagtc tccagcactg gatgttaccg ttcacgcaat tggtaagacc cgcagtatcg
     1621 agcgtatcaa caaagcgctg gattttattg ctgaacgcga aaatcagcag taattagcgt
     1681 gtaaaaagat aaacggcagg agataatatc ctgccgtttt ttatttatgc tgattcgtca
     1741 atatcgagac gggcgaggaa attgcgaatt ccttcacgtg acaaaaattg cgcgactttt
     1801 tcctgctcaa tgtctgtcat attatctatc gcgttgatga tttgtcgata cgccggggcg
     1861 cgtgctccag gagcgtattc agcaattctc tcttcagctt ccatcaacat tggtgtgtta
     1921 tggaaggctg gcgtgttctg aataaactcg caaacttgcg tatcgaccag aataagtctg
     1981 gcacgaccgc ctttcacgcc gggaaatttt tccgttttcc agcctttttc tctgatccaa
     2041 cggttaacag tttgtttagc cacaccgagg cattcagcca gttcttcggt ggtcatttta
     2101 ttgcgtaatc ttttcatatt atttgctatc gcgaatccct aagcgttgta acaatccgat
     2161 aatcccttcc cgcagaagca aggatgtaaa ctgtttttgt tcaactggcg tcatctcttt
     2221 cgccagtgtc accagcaata cttcaagcgg cgcatcacca gaaagggcag gcgcttctcc
     2281 ctgaccttct ggacgttcag cattgcgaat atattcacga acctgttcat tgacgtgaac
     2341 cagtcgggct ttaccaccct ggacgccagg ttttggtgac gttgtccagc cttccttgcg
     2401 tacccattta ttaatggtct ggcggctata gccggtcagt ctggcaagtt catctggtgt
     2461 catcctctcc ttggacataa caattccctg aatgtatgtg ggtctattag tagttcattt
     2521 tataacacca ctttacagga aaccgtgaca tgaataacta ctgaatgcga gcggttttcc
     2581 aaaattgttc atttgcctga tttttcagcg attgaaacta cacgttgcaa tttgccgttg
     2641 acacattcgg gcggaattca tatgatgccg cccgtcaact cgacaagctt tacgtga
//
LOCUS       DADLTF010000274         2671 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.279, whole genome shotgun sequence.
ACCESSION   DADLTF010000274 DADLTF010000000
VERSION     DADLTF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.279"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(44..991)
                     /gene="icsP"
                     /locus_tag="JN749_004167"
     CDS             complement(44..991)
                     /gene="icsP"
                     /locus_tag="JN749_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HAY7370344.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(2372..2524)
                     /locus_tag="JN749_004168"
                     /pseudo
     CDS             complement(2372..2524)
                     /locus_tag="JN749_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2580..>2671
                     /locus_tag="JN749_004169"
     CDS             2580..>2671
                     /locus_tag="JN749_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HAY7370345.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
BASE COUNT          821 a          514 c          456 g          880 t
ORIGIN      
        1 gcaattttta gccatgaaaa cgtaatcaac tcggtgaacc aattcaaaaa atatacttta
       61 tacctgcgga agtgagaaaa ccaatatatt caatacttgc actattttta atggtgccag
      121 atatattggt actttgctca taaagagatg tatcaccttt tttattagaa atgtaatttc
      181 tggagccttc tatataaaat tttgcactgg gggtgacata gtatccaata ttcgcagcaa
      241 cagagcagta attttggttt tttatttcat ctttaaaaat tgtctcagtc tgatagtgtt
      301 tatctgtatc agatgaaaga acccaaccac tatatttcag ttctgctcca aactcaaaat
      361 tctcatggcg gtaattagca gttaatccaa tataaggtat tttgaagagc tgtttgtaac
      421 ctattgttct ttcaccacta ggatgtgccc cttttttatt cctgttcccc ccgttttcac
      481 tataaatata actccctccc attgcattaa aactgtaacg actctcttgg taacctgcta
      541 ttagtccaag tcgataatcc aaattattta ataaccaacc tttaagattc agctcgaatt
      601 cgttagcatc acggagatgc gtattgggat gccagctctg gtcggtccat acctgagggg
      661 tattggaatt gttccagtct ttatcaacca tgcttgcttt ctggttacct aaagtagtcc
      721 aaccggaaac tccgaaagac acttttggaa ttagcttcca atcgatgcca cctctaacaa
      781 tagtcgcatt actgtatttc cagtccagtt gactaatttt tctcccccct tccttgggat
      841 gataaacgcg ttcttttgtt ttgccactca gagatcctag actaatatcc gtactgatgt
      901 tgtccggtat gaaaagtgga tagttagtgg tagcatgtgt agtaaagatc gcaggtacac
      961 aaagtgcaag tacaaggaat tttgatttca tcgagaactc tacttttttg gttgaaatgt
     1021 ccatgatctt cttactttat aagataaacg tcaatgccaa gcattatttt aattaaatat
     1081 ttgtttatgt taccaagaac atatctttta ttataatatt tattgaactt ttgtaaaaat
     1141 aaaaaaatac ctcattgtgc gaataaagta acgggggcca tagatttctc tacctacctt
     1201 ttggtgttca tacctgaggc tgatagtttc aggattccgc gacaattcta ctcaccttct
     1261 tgttctctcg ccaatatgaa atcaatcaga aaagcgttcc ataagaacaa cgataagaca
     1321 ataaattgtc atcacggctg caatagccaa cgtgcctttc agtaccaaca tgacaacctc
     1381 ctgtatttga cgtaacgtgt gctgataaat atgtatttgt atctcaactg acttaataca
     1441 acacttaagt atagtttttc agattttgtt tttcccgtga aatattatgg aaatggcaga
     1501 agcaatacat tgaattccga ttcgagtgaa tacggctagc gggcacctca ctttagcact
     1561 gaagccatca acttttattt gacttatttc aatggaaagc tttctcacta tcagtacagg
     1621 taattcccat ccttttaaag tcgagagtca gactgcgact aatacagtta aagtgaccaa
     1681 actcagttat cctctacaca ttttacctca tgtttacttg gacaattcat tggtgctttt
     1741 ataaaagaat aaatattcta ctaatttaat acatcaccta aaatagtatt ttttataata
     1801 gaactgtatc tatagtatat tattaaagct taattattta ttatgcttta atgtggttgt
     1861 ccgattaagg actaatcctt gaaaacgccg tatagggcgg gagttcactc tattgcgata
     1921 acgtaaccgt acaatattgt gcgtttccca aattgaccac tactgaacta ttctgggatt
     1981 taaatgcaat aaaatggttg gttgaaggtc gtggtgatga tgttagtcct tttgagaata
     2041 ttccagtgcc agatcttact tataataaag aggcttggca gtttggataa gctattgtcc
     2101 ttattccact aatacaaact ttttgtaaag tatattaaat atacttcatt tcatactgaa
     2161 atccccggaa gtattcaatc aaacaattac accataaaag gaagagggag ccccaattaa
     2221 gttatcaagc atgtttatga tacctgcagg gtataaattt tctggctgca agcagacaca
     2281 tataaaacgt ctttattgat cctacccaca taatatggac gcagtcgtaa gtgaggctct
     2341 gtgagcctac ccacgtaata tggacacagg cctaagcgag gttctggttt tcaaattgtt
     2401 ccggactgag gccgccacac caacggtgcc gtcgccagcg attgtaatcg cactcgatat
     2461 aattaaacac tgttgcccgc actatttccc gatagataaa gtgttctccg tggagacatt
     2521 ccacttagga acaattatat tgttcatggt aatgtttata tttgagtata gagattaaaa
     2581 tgattagtcc catcaagaat attaagaacg tatttccaat aaatactgca aatactgaat
     2641 atatagtgag aaatatttat cctagagttg a
//
LOCUS       DADLTF010000275         2646 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.280, whole genome shotgun sequence.
ACCESSION   DADLTF010000275 DADLTF010000000
VERSION     DADLTF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.280"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..542
                     /locus_tag="JN749_004170"
     CDS             <1..542
                     /locus_tag="JN749_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HAY7370346.1"
                     /translation="QVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQ
                     RTKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQK
                     CMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLL
                     LLIRFLMHARNSGVLKWKA"
     gene            543..908
                     /locus_tag="JN749_004171"
     CDS             543..908
                     /locus_tag="JN749_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAY7370347.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            905..1621
                     /locus_tag="JN749_004172"
                     /pseudo
     CDS             905..1621
                     /locus_tag="JN749_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
     gene            1815..1890
                     /locus_tag="JN749_004173"
     tRNA            1815..1890
                     /locus_tag="JN749_004173"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1848..1850,aa:Ile,seq:cat)
     gene            1892..1968
                     /locus_tag="JN749_004174"
     tRNA            1892..1968
                     /locus_tag="JN749_004174"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1926..1928,aa:Lys,seq:ttt)
     gene            1971..2046
                     /locus_tag="JN749_004175"
     tRNA            1971..2046
                     /locus_tag="JN749_004175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2004..2006,aa:Thr,seq:tgt)
     gene            2050..2124
                     /locus_tag="JN749_004176"
     tRNA            2050..2124
                     /locus_tag="JN749_004176"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2083..2085,aa:Gly,seq:tcc)
     gene            2417..2632
                     /locus_tag="JN749_004177"
     CDS             2417..2632
                     /locus_tag="JN749_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7370348.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          697 a          575 c          713 g          661 t
ORIGIN      
        1 atcaggtggc ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg
       61 aaaaaatccg ctcaatgtac cgtgaaagcg acatcgtgcc gggagagcag accgccacca
      121 gcatactgaa gcagcgcaca aaaaatcttg cgccgctgcc tcacgcccac cagcaacaga
      181 atccaccaca ggaagagacg gtggtcagca ttgccgttga tccggaatct ccggctcagt
      241 atctccagcg ccagaaacca caacgggaag agatgcctgt atacacgcgc tgggtaaaaa
      301 cgcagaaatg catgacgtgt ggcaatcagg cagatgatcc gcaccacatc attggtcatg
      361 gactgggagg gatgggaaca aaggctgacg atttgtttgt tattccgctg tgtcgtaaat
      421 gccataacga actacacgcc ggggtaaaag attttgaaga aaaacacggt agtcagttgt
      481 tgttgctgat tcgtttttta atgcacgcga gaaattcggg tgttctgaag tggaaagcat
      541 aaatgaccga acgcatagaa tttgttttgc cttacccgcc aacggtgaac acctactggc
      601 gacgtcgtgg cagcacatat tttgtatcaa aagccggtga gcgttatcgc cgtgatgtgg
      661 cgctaattgt tcgccagcag cgactgaaat taaacctgtc cggaaggctg gcgataaaga
      721 ttattgcaga gccaccggat aagcgccgtc gtgacctgga caatatcctg aaggcaccgc
      781 tggatgcgct gacgcatgct ggactactta tagacgacga gcagtttgat gaaatcaata
      841 ttgtgcgcgg tcagctcgtt cctggtgggc ggctggggat aaaaatcaca gaactggggt
      901 gcgcatgaat aaccagtatt tacagtttgt gcgtgagcag ctcattatcg ccaccgctga
      961 tttgagtggg gcaacaaaag gtcagcttga agcctggcaa gagaatgcca tgttcgatac
     1021 agggcgttac aggcgtaaaa aaatccggta ccgcgatgaa gtgactggaa aaatgataac
     1081 gcgggataat ccaccaatcc cgggaaagca atcgctggcg aaggggacgt caattcctct
     1141 ggtcagtccg gttgagtttt cgacatcatc gtggcggcgg gctgttctgt ctcttgaaga
     1201 acatcataaa gcctggttgt tgtggtgtta cagcgggagt atttgttggg aatatcagat
     1261 cgcgataaca cagtgggcgt ggaatgaatt taatgctcaa tccggtacca gaaaaattgc
     1321 agggaaaacg caggaacgcc tgaaaaaatt aatctggctg gcggcgcagg cagtaaaagc
     1381 agaacttttt ggtggggaag gttatgaata ccaggttggt ggggaaggtt atgaatacca
     1441 ggagctggca tttactggcg ggagtgacaa ctaaaaactg gtccaaaaca tttactcgtc
     1501 actggattgc aatgaaacac atttttcacc gactggatag tgaggcttta ttgtttgtaa
     1561 tgagaacacg ttcaaaaaaa agacggcatt ttcaaagcaa agtgttgcaa aagtagattg
     1621 aaaggcatat attcatgcaa atctgatatt ttgccgattt tttacgtgat ggcaaaagca
     1681 aacaaaaccc gcccacaagc gggttttttg tgccacttat ctcggataga catggtgaat
     1741 gcgctggtgg aggagataag ggtgattttt gaatgcttgc aacattgatt tcgtaacgtt
     1801 attatcctgc gcccggccct ttagctcagt ggtgagagcg agcgactcat aatcgccagg
     1861 tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg aaagagcgtc
     1921 aaccctttaa gttgagtgtg cgaggttcga gtccccggtg gcggtccagt gccgacttgg
     1981 ctcagtaggt agagcaactg acttgtaatc agtaggtcac cagttcgatt ccggtagtcg
     2041 gcaccatatg cgggcatcgt ataatggcta ttacctcagc cttccaagct gatgatgcgg
     2101 gttcgattcc cgctgcccgc tccagttaga gtctttcagt ctgcgatgat gggaaatccc
     2161 ggagtgactg aaagacgttt aagttatgaa tgatcgcttt tttttgcaaa attgctgtgc
     2221 agaaatacta accttcgggc aggcgatcat tcataagcac tctgctttta ttccgattaa
     2281 ctgtgagtgg tttgttggat agagtgcttt ccttactgta tatatcgttt cgcccgcttt
     2341 tgcggttttt tcttttcaaa tccctttcat ttctcagtgt aaaactacgc catccgttat
     2401 ttgcggaggt gaggctatga aatccatgga caaaatttca acgggcattg cctacggcac
     2461 ctccgcaggc agtgctggct actggttttt acagtggctt gatcaggtca gtccgtcaca
     2521 gtgggctgcg attggtgtac tggggagtct ggttctgggc ttcctgactt atctgacaaa
     2581 tctgtacttc aaaatcagag aagacaagcg taaggctgcg agaggtgaat aatgtcgcca
     2641 tcatta
//
LOCUS       DADLTF010000276         2642 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.281, whole genome shotgun sequence.
ACCESSION   DADLTF010000276 DADLTF010000000
VERSION     DADLTF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.281"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..247
                     /locus_tag="JN749_004178"
     CDS             <1..247
                     /locus_tag="JN749_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370349.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JN749_004179"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="JN749_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HAY7370350.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWLIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JN749_004180"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="JN749_004180"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAY7370351.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..>2642
                     /gene="mdfA"
                     /locus_tag="JN749_004181"
     CDS             1991..>2642
                     /gene="mdfA"
                     /locus_tag="JN749_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HAY7370352.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLK"
BASE COUNT          673 a          644 c          684 g          641 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taagccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 ga
//
LOCUS       DADLTF010000277         2591 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.282, whole genome shotgun sequence.
ACCESSION   DADLTF010000277 DADLTF010000000
VERSION     DADLTF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.282"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(122..1135)
                     /locus_tag="JN749_004182"
     CDS             complement(122..1135)
                     /locus_tag="JN749_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAY7370353.1"
                     /translation="MMTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEES
                     SYRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKH
                     SAEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSV
                     WCDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLI
                     ANGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGF
                     DDIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIA
                     LSR"
     gene            1392..>2591
                     /gene="ascF"
                     /locus_tag="JN749_004183"
     CDS             1392..>2591
                     /gene="ascF"
                     /locus_tag="JN749_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HAY7370354.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICG"
BASE COUNT          598 a          695 c          653 g          645 t
ORIGIN      
        1 cgccgataca ttcacaaatc atgccagttt ttaatttgct gttatttaag gaaattaatt
       61 tctgtaatgc aggaaaaaag atgtcatcga cactagtgac gatgacatgt gatgacaatg
      121 tttatcgcga aagggcaatg agtgagtcgc ggcggatcag ttttccgcta aaggttttag
      181 gcggtgagaa atccccgcca tcgagcataa aaatcagccg tccaataatt tcctgaatca
      241 tctcagtcac cggaattttt acgctggaga gcgccggaac gatatagggg gcaattgcga
      301 tatcatcgaa tccgataact gacacctgct ctggcaccgc tacgccgcgc tcgtgcaacg
      361 ctttgatcgc acctatcgcc atatcgtcgt tactggcaac taacgcgcta aatttagccc
      421 cacgttcgag caacgtttct accccttcgg ccccgctggc gggcgtccat ttaccgttag
      481 cgataagttt ttcattgagc gcaataccat gctgcgacag cgcgtcttta tacccggcaa
      541 gacgttcaat gctggtgggg gaatccatcg agccggtaag gaaagcaatc tcctgatgcc
      601 cggcgtttat caactctgcc acggcgttaa agctggtctg tttatgatcg caccagacgc
      661 tatggctgct attattgcgc aggcggcgat taagcaccat tatcggctga ctgtgcgcgt
      721 caatgatgtc atcaatctca tccacgctta aaaaacgcgg gtaaatcata atggcgtcgc
      781 agcgcaaatc cagcagatac tgaatcgcct ggcgctcttc ttcggcgctg tgtttaccat
      841 ctgccaatag caactgtcgc cctttctctt ccgccattcg cgcggcatga aacagtaatt
      901 cactaaaata aatgccgtga taaagcgtgt tggtcactac cagccccagc gtctgagtac
      961 tcttcgccga cagattgcgg gccagcaaat ttgggcggta actgctctct tctaccgcct
     1021 gaaacacgcg atctttagtc tcctggctga cgtagccatt acctgaaagc acgcgggaaa
     1081 cggtcgcttt tgaaaccccg gcgcgcttcg ccacttccag catcgtcgtc atcattttca
     1141 tccctttaca cgcaatcaac gcagtgtact gcaccgtttg ccgattgtcc ttgcgcaatc
     1201 ggcgggaaaa atattcaggt gaccggtttc acaaatataa aaaatgaaca attaactctc
     1261 ttgcttattt agtgacaact attcatgatt ttgtgaaacc ggtttcttaa ttccgtttca
     1321 gcatcggtat ttttccgtca cgtcgactga taacaactac atctacccta ctgataacag
     1381 gataaaatcc gatggccaaa aattatgcgg cgctggcacg ctcggtgata gcggcactgg
     1441 gcggcgttga taacatctcg gcagtcacgc actgtatgac gcgtttgcgc tttgttatca
     1501 aagatgacca gcttatcgac agcccgacgt taaaaaccat ccccggcgtg ctcggcgtgg
     1561 tacgtagtga caaccagtgt caggtaatta tcggcaatac ggtttcgcag gcctttcagg
     1621 aagtcgtcag cctgctgccg ggagatatgc agcccgcaca gcccgtgggt aaacccaaac
     1681 tcacgctgcg tcgcattggt gcggggatcc tcgatgcgct gatcggcacc atgtcaccgc
     1741 tgatcccggc gattatcggc ggatcgatgg tcaaactgct ggcgatgatc ctcgagatga
     1801 gcggcgtgct gacaaaagga tcgccgacct taaccattct gaatgtgatt ggtgacggtg
     1861 ctttcttctt cctgccgcta atggtcgcgg catctgccgc catcaaattt aaaaccaata
     1921 tgtcgctggc gattgccatc gcgggtgtgc tggtacatcc gagttttatt gaattaatgg
     1981 cgaaagcggc ccagggtgaa catgtcgaat ttgccctgat cccggtcacc gcggtgaaat
     2041 acacctacac ggtgatcccg gcgctggtga tgacctggtg cctgtcatat atcgaacgct
     2101 gggtggatcg cattacaccg gcggtgacca aaaacttcct caagccaatg ctgattgtgt
     2161 tgattgccgc accgctggca atcctgctga ttggcccgat tggtatctgg atcggtagcg
     2221 ccatttcggc gctggtttac accattcatg gttatctggg ctggctttca gtcgccatta
     2281 tgggcgcgct gtggcctctg ctggtaatga ccgggatgca ccgcgtcttt acgccaacca
     2341 tcattcagac cattgccgaa accggcaaag aaggaatggt catgccatca gagatcggcg
     2401 ctaacctgtc gctgggcggt tcatcactgg cggtgccgtg gaaaacgaaa aacccggaac
     2461 tgcgccagac ggcgctggct gcggcggcat cagccattat ggcggggatt tccgaaccgg
     2521 cgttatacgg cgtggcgatc cgcctgaaac gtccgcttat cgccagtctt atcagcggtt
     2581 ttatttgcgg c
//
LOCUS       DADLTF010000278         2568 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.283, whole genome shotgun sequence.
ACCESSION   DADLTF010000278 DADLTF010000000
VERSION     DADLTF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.283"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(344..610)
                     /gene="ospE2"
                     /locus_tag="JN749_004184"
     CDS             complement(344..610)
                     /gene="ospE2"
                     /locus_tag="JN749_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HAY7370355.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(792..1193)
                     /locus_tag="JN749_004185"
                     /pseudo
     CDS             complement(792..1193)
                     /locus_tag="JN749_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1622..2410
                     /gene="virF"
                     /locus_tag="JN749_004186"
     CDS             1622..2410
                     /gene="virF"
                     /locus_tag="JN749_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HAY7370356.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2352..>2568)
                     /locus_tag="JN749_004187"
     CDS             complement(2352..>2568)
                     /locus_tag="JN749_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7370357.1"
                     /translation="EEYDFTFATGAPQKQLQSLRSLSVIERNENIVLLGPSGVGKTHL
                     AIAMGYEAFKIFYDISKISLELYHNIH"
BASE COUNT          773 a          471 c          485 g          839 t
ORIGIN      
        1 cccctaagcc gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat
       61 atatttctcc atctatgact tgtcaatacc atcctgatat ttgtgggtca gcaatatatt
      121 ctactaaaag gtatttaagg tcattgcgtc cctgcactcg aataatggtg aagaacgcat
      181 gataaatata tcacgataac gttcagtatg tatcgaggat agatggagtt actcctctct
      241 gaaatagaat tactgctcta ctttttctgt ttccagaaat tattagtaac aaaccctgat
      301 atattatttt gaaaatggag cttaaaccag tctgaaatta acttcagaaa tcagaatacc
      361 gttggctttg tttttcatca attccggctt catttatctt tgtcaaccat tgcatttctg
      421 tagcagtgaa tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat tcgtatatag
      481 ctgctttttt attgaaaaca gtatatccat ctgtagtaac agtagaagat aataatacag
      541 gatttgatac ttcaaggatg atattttctt gtttttgtgg aaggcaaggg aatatagttt
      601 gtgtaagcat attgcttcct tatgttatat tttagtaaag aatttatttt ttacattata
      661 gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg atgagatgtg
      721 gagccagaaa aatggctgcg ttacgtcatt gaactgtcct tgacccacga tattcagaca
      781 gtcgttgtat cttaagttga cattacccgc tgacgtcgat attcccttgg tgagagataa
      841 cccaacgcac tatgcggatg gttttcattg taatgctcga tcgccactgc aaggttatgc
      901 aatgccgttc ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt tttcacgaac
      961 cgttctgcta ttccattact ctgagggcta ctgactgccg tcgtgcaggg ctccagattc
     1021 aactctctgg cgaactgccg tgtttcatga gccgtataag ccgaaccgtt atcggccact
     1081 ggattggccg ttccggtcct ttatcgccat atcactcctg acatgacatc ctgcaccgtt
     1141 gctttgtcat aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga
     1201 acgtaacgct gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct taccaatatc
     1261 aacaccaacc ggatttgtgg agcctccagt ctgaaggggc tttatgcgtt ccgtatagga
     1321 tattatgatg ctggagtttt gcgaagctcc actcctttcc gaacatttac acagatattg
     1381 cctaagaaaa gtgaaaccat aatatggtta tagtcccttt cagtgcaaat acttagcttg
     1441 ttgcacagag aaatagaagc tgcataagct ctttcttcaa aaaatgtaaa taaagttaaa
     1501 tataggaaaa attacttaat ctatcttaat aacggaaaga ttttgtatac aatcactgtt
     1561 acagaaattt cttagttact ctgtaaacac taaatatagt ttggtatatt ctgttgaatt
     1621 tatgatggat atgggacata aaaacaaaat agatataaag gttcgcttgc ataactatat
     1681 tattttatat gcaaaaaggt gttcaatgac ggttagctca ggcaatgaaa ctttgactat
     1741 cgatgaaggg caaattgctt ttatagagcg aaatatacaa ataaacgtct ccataaaaaa
     1801 atctgatagc attaatccat ttgagattat aagccttgac agaaatttat tattaagcat
     1861 tattagaata atggaaccaa tttattcatt tcaacactcc tattctgagg agaaaagggg
     1921 gttaaacaaa aaaatattcc tcctctctga ggaggaggtt tctatcgatt tgttcaaatc
     1981 tataaaagag atgcctttcg gcaaaagaaa gatctatagt ttagcttgcc ttttatcagc
     2041 tgtttctgat gaggaagctt tatatacttc gatatcgata gcttcttctc ttagtttttc
     2101 tgatcagata aggaagattg ttgaaaaaaa catcgagaag agatggcgtc tttctgatat
     2161 ttcaaataac ttgaatttat cagaaatagc tgttagaaaa cgattggaga gtgaaaaatt
     2221 aacatttcaa caaatccttc ttgatattcg catgcatcat gcagcaaagc ttttattgaa
     2281 tagtcaaagc tatattaatg atgtatcaag acttatcgga atatcaagcc catcttattt
     2341 tataaggaaa tttaatgaat attatggtat aactccaaag aaattttact tatatcataa
     2401 aaaattttaa atgcttcata gcccatcgct attgccagat gggttttccc cacacctgat
     2461 ggccccagta atacgatatt ttcattacgt tctatgacgc tgagtgagcg taacgactgg
     2521 agttgcttct gcggtgctcc ggtggcgaat gtgaagtcat actcttcg
//
LOCUS       DADLTF010000279         2503 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.284, whole genome shotgun sequence.
ACCESSION   DADLTF010000279 DADLTF010000000
VERSION     DADLTF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.284"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            155..1108
                     /locus_tag="JN749_004188"
                     /pseudo
     CDS             155..1108
                     /locus_tag="JN749_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JN749_004189"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="JN749_004189"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HAY7370358.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVASYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTATLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
BASE COUNT          691 a          614 c          562 g          636 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgcatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gagtggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aatacgcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaagc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttac tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgcca cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DADLTF010000280         2489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.285, whole genome shotgun sequence.
ACCESSION   DADLTF010000280 DADLTF010000000
VERSION     DADLTF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.285"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="JN749_004190"
     CDS             complement(<1..646)
                     /gene="yidZ"
                     /locus_tag="JN749_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HAY7370359.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
     gene            complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="JN749_004191"
     CDS             complement(621..1796)
                     /gene="mdtL"
                     /locus_tag="JN749_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HAY7370360.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            complement(1928..>2489)
                     /locus_tag="JN749_004192"
     CDS             complement(1928..>2489)
                     /locus_tag="JN749_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HAY7370361.1"
                     /translation="VVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKS
                     FLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTF
                     LPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIP
                     AIVILFGITVILCWFGNVFNVLPKFG"
BASE COUNT          712 a          663 c          636 g          478 t
ORIGIN      
        1 aggtgtcgct ctgttcccag caaatgctga tatgcggata acgtaaaaag gtgtccagat
       61 tccacgtttc atgcagtgcc ggatgatctt tgcgtaacca gacgcagggt acatcactaa
      121 acagcacttc ataatcaatg gctaacggta gcgagcttaa cagctcccgc gagcgaggat
      181 ggctttcgcg accggaaaaa ccaatatcca cttcaccacg agtaatggca tctaaggaat
      241 cgtaatccca gttacgtagt ttgatggtcg cctgcgggta acgttggtag atccgtttcg
      301 acagcgcatt aagcatgatc atcatcagcg gcgattccgc cgccaactca aacttcaggc
      361 cgcgcggtgt ctggtggtgc ggtttatcca gcagcagatt gctcatttgc atccattccg
      421 ccagattttg ctccatgctg accattagct gtgtgggcga cagccccagc ggtgagttca
      481 caaagagcgg gtcgtcaaac cacgctctta actttgccag cgacttactc accgctgaag
      541 gggtcacgtt catccgcttc gccgctttgg tcacgctgcg ctcctgcatc agcagttgca
      601 gacagagcag cagattgaga tcaagcgtgg tgatggattt cttcatgagc ggcaacgggg
      661 cgtccaggcg cgacgaacat aatcagcaac aggctcacta tgctacaggc aatcagaatc
      721 ccgatcagca tattccatgc gccgatacca accaccgcgg ccagccaaat ccacagtgac
      781 gaaccgcaaa cctgcgcaat acctaaggtc gagctggcta cgcccgcgcg taatgaaaat
      841 ggtcctaacg cctgactcat cgctacgcca aaacctaccg agaaaccggc gcaaatcagc
      901 gtgataccaa acagagaaac cgcatgggaa ggtgaaacgg caagggtgat ccccgccgca
      961 aggaataaca cctgcgaggt gatcatcaac gtacgtggct taaaaattcc cagcgcaaat
     1021 ggcgtcgaga atgaaaaggt catgctgacg ccagcggtca gcgccataat ggtggcgtat
     1081 tctccgcgct caaaccccat gatttccatc agcaataccg gcgatgtatt gacgaaagtg
     1141 aggatcaccg aaacgctgag ggtggtgata acaacacggc tgaggaaaaa acgattaagc
     1201 agcgactcgc tattttctcg tggtttgtcc gaagcagcgg gggacgctgg gcgcgtttct
     1261 tttaaaataa acaaagacaa catcagtacc gcgatgccca tcattgccat cgcccagaac
     1321 agactctgcc acgggaattt aagcataatc agatgtccga gcactggcgc taacaccgga
     1381 atgatgcagg taataccgtt gagtaatgac agcactttag cccgacgtcg atcatccagc
     1441 gtgtcgcgca aaatggcgaa cgccaccacg taacaacagc ctgcgcccaa cccctgtaga
     1501 aatcggcctg caagaaataa cgtgctggtt tcagccagtg aacagaacac cgaggcaata
     1561 ataaatagcg ccgcgccggg tatggcgacc ggctttctcc ctgaacgatc ggccacttta
     1621 ccggcaaata gcatcgcagc tgccatcccc gccagatata cggagaacgc aatatgcaac
     1681 tgcgcttcgc tggcattgag atcggcggcg atgcgcggta aaccaacgag gtacatatca
     1741 atcccggcgg gataaagtaa aaccagggca aaactacaaa tcaaaaagcg ggacatgggc
     1801 taaagcgtcc tgatagtaag gtgacgcaaa gcatacgtgg tgaaggagga taaggcgagt
     1861 tgccatttgg acataagtga tttcctgaga ggcaagaagc cagcgaatgg ctggcttctt
     1921 gaaggattta gccaaattta ggtaacacgt taaagacgtt gccgaaccag cacaaaatca
     1981 cggttatacc aaagagaatg acaatcgccg gaataagatt gccgccccag accgtgaaca
     2041 tctgattggg aaacttcttg cgagctttga ttgcaagcac tgcgggaata atgaccgccc
     2101 agatggtggc gcacagcccg gcaccgccga tcccgtaaat aaagccgttc gggaagatca
     2161 gatacaacaa cgcaggtggc aggaaggtta acagcacggt tttgaaacgc ccgccgtggg
     2221 agttatcaat cttaaacagg tccgccagat aatcgaacaa ccccagcgtg acaccaaaga
     2281 acgaactggc aacagctaag ttagagaaca ccagcaggca aaactcgata atgccgtgct
     2341 gtttggtgcc gaggaacgat ttcaccagcg aatcaacgtt gccgcctgag gagattatcg
     2401 ccttaaagct ttcgcgcgga atattcccca tcgtgcaata gagccagaag agataaatca
     2461 ccagcgccag cagcgaacca aataccacg
//
LOCUS       DADLTF010000281         2458 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.286, whole genome shotgun sequence.
ACCESSION   DADLTF010000281 DADLTF010000000
VERSION     DADLTF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.286"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            30..1082
                     /locus_tag="JN749_004193"
     CDS             30..1082
                     /locus_tag="JN749_004193"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HAY7370362.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1158..2458
                     /locus_tag="JN749_004194"
                     /pseudo
     CDS             1158..2458
                     /locus_tag="JN749_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          583 a          552 c          634 g          689 t
ORIGIN      
        1 agttatgttt ttaaattgag ggcattatta tgaaaaaaat accctgcgtg atgatgcgag
       61 gtggaacctc gaggggcgcg ttcctgttag cggaacattt acccgaagat caaacgcagc
      121 gcgataaaat attgatggca attatgggtt ccggtaacga tctggaaatt gacggtattg
      181 gcggcggtaa tccactgacc agtaaagtcg ccattattag ccgttccagc gatccgcgtg
      241 ctgatgtcga ttatctgttt gctcaagtta tcgtccatga gcaacgtgtc gataccacgc
      301 ctaactgcgg caatatgctg tctggcgtag gggcattcgc cattgaaaat ggtttgattg
      361 cagcgacttc gccagttacc cgtgtacgta tccgcaacgt caatacgggt acgttcatcg
      421 aagctgatgt gcaaacgcca aatggtgttg tcgagtacga gggtagcgcc agaattgacg
      481 gcgtaccggg tactgccgca ccggttgcgc tcactttcct gaatgccgct ggaacgaaaa
      541 ccggaaaagt tttcccgact gataatcaga ttgattattt tgacgatgtc ccggtgacct
      601 gtatcgatat ggcgatgcca gtcgtcatta ttccggctga atatctggga aaaacaggtt
      661 atgaattacc ggcggaactg gatgccgaca aagcattatt agcccgcatt gaatctatcc
      721 ggctacaagc gggtaaagca atgggcttag gtgatgtcag taatatggtt atccctaaac
      781 ctgtgcttat ttctccagcg cagaaaggcg gggcaattaa tgtgcgttat tttatgccgc
      841 attcttgcca tcgcgcgctg gcgataaccg gtgctattgc tatttccagt agttgtgcat
      901 tggaaggcac cgtcacccga caaatcgtcc cttctgtagg atatggcaat atcaatattg
      961 aacaccccag tggtgcgctc gacgttcatt taagtaatga aggtcaggat gccacgacgt
     1021 tacgcgcatc tgttattcgg acaaccagaa aaatattttc cggtgaagtt tatcttccct
     1081 gaaaaaattc gttgtcagga taaggacaat caataaagga cttctgtatg agtcatacag
     1141 aaagaacagg attttaaatg aataagaaat cgttatggaa gctaattctg atattagcga
     1201 tcccatgtat tattggtttt atgccagctc cggcaggatt aagcgaactg gcgtgggtgc
     1261 tttttggtat ttacctggcg gccattgtgg gtctggttat caagcctttc ccggaacctg
     1321 tcgtactgtt aattgccgtt gctgcctcaa tggtggtggt cggtaactta tccgacggtg
     1381 cgtttaagac caccgccgta ttaagcggtt actcttcagg tactacctgg ctggtgttct
     1441 cggcgtttac cttaagcgcc gcatttgtga ccaccggttt aggtaaacgt attgcctatc
     1501 tgctgattgg taaaatcggt aacaccatgc tgggtctggg ttacgttacg gtattcctcg
     1561 atctggtact ggctccggca acaccgtcta acaccgcgcg tgcgggcggc attgtgttac
     1621 cgatcatcaa cagcgtggcg gtggctttgg ggtccgaacc ggaaaaaagt ccgcgtcgtg
     1681 tcggacatta cctgatgatg tccatttaca tggtcaccaa aaccaccagc tatatgttct
     1741 ttaccgcaat ggcggggaac attctggcgc tgaaaatgat caacgacatt ctgcacctgc
     1801 aaattagctg gggtggatgg gcgctggccg ccggattgcc ggattgccgg attgccggat
     1861 tgccggattg ccgggcatca ttatgctgct ggtcaccccg ctggtaattt acaccatgta
     1921 tccaccagaa attaaaaagg tggataacaa aaccatctct aaagcgggcc ttgccgaact
     1981 gggaccgatg aaaatccgcg aaaaaatgct gctcggtgtc tttgtgctgg cgctactggg
     2041 ctggattttc agtaagtctc tgggggttga tgaatccact gtggcaatcg ttgttatggc
     2101 gaccatgctg ctgctgggta tcgttacctg ggaagacgtg gttaaaaata aaggcggctg
     2161 gaatacctta atctggtacg gcggtattat cggcttaagc tccttattat cgaaagttaa
     2221 atttttcgaa tggttagctg aagtctttaa aaataacctg gcatttgatg gtcacggtaa
     2281 cgttgctttc ttcgttatta ttttcctcag catcatcgtg cgttatttct tcgcttccgg
     2341 tagtgcctat atcgttgcta tgttacctgt atttgccatg ctggcgaacg tctccggcgc
     2401 accgttaatg ttaaccgcgc tggcactgtt gttctccaac tcctatggcg gcatggtt
//
LOCUS       DADLTF010000282         2439 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.287, whole genome shotgun sequence.
ACCESSION   DADLTF010000282 DADLTF010000000
VERSION     DADLTF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.287"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..564
                     /locus_tag="JN749_004195"
     CDS             <1..564
                     /locus_tag="JN749_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAY7370363.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            522..1199
                     /gene="bglJ"
                     /locus_tag="JN749_004196"
     CDS             522..1199
                     /gene="bglJ"
                     /locus_tag="JN749_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HAY7370364.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQEFFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="JN749_004197"
     CDS             complement(1237..2025)
                     /gene="fhuF"
                     /locus_tag="JN749_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HAY7370365.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2166..2402
                     /locus_tag="JN749_004198"
     CDS             2166..2402
                     /locus_tag="JN749_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HAY7370366.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          627 a          532 c          627 g          653 t
ORIGIN      
        1 gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga acattattat
       61 tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg ctcctggtat
      121 tcccaggctg tagaactgct catgtgccct acggcgacgt tattgtctga tgttgaaccc
      181 attgagaatc tggtcaagac cgtacgttcc ggcaatacgc atgcagagcg tatcagcgcc
      241 atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc cgtcattctc
      301 actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat caaccagata
      361 gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag tgcgatgcgt
      421 cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc gcagggtgca
      481 agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac agccgaatta
      541 agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag tagtatcggt attgagagtt
      601 tattcagaaa gtttgcgggc aacccttata agctccatac ctataccagt caggagtcat
      661 ttcaggatgc catgtcgcgg atctcgtttg cggcggtcat tttttctttt tctgccatga
      721 gaagtgagcg gagagaggga ttatcttgcc tgactgagct ggcgattaag tttccgcgta
      781 cccggcgttt agttattgtg gatgatgata ttgaagctcg gctgattggt tcattgtcgc
      841 catcaccgct ggacggtgta ttaagtaaag cgtcaacgct ggagattttt catcaggaat
      901 tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat cagtggtaca
      961 ttaaccaaag ccggacgtta agcccgacgg agagagaaat attgcgcttt atgtcgcgtg
     1021 gctactcaat gacacaaatt gccgagcagc ttaaacgtaa tatcaaaacg atccgagcgc
     1081 ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg ttggaggccg
     1141 cagatattct gttatgtatg cggcattgcg aagcaagtaa tgtgttgcat ccctattaat
     1201 ccgcatgatg ccgggtttac ttccccggca gtgctttcat ttcagcgtac aatagccaca
     1261 ttgctgcaca tccggtaagc gataacgctg gcagcaagtg cggcgcacca gcaggccgtc
     1321 gcgcagtacc acggtacgcc agagtggatt atcttcaccg ttcgtgagcg ttttctcaaa
     1381 aaagagggca tggcgcagcg attcaacagt agcctcgccg agcagttgct tcatctcagt
     1441 gagataccag ttgatcaaat aaccggtatt actccagata agtttgccgt tgatctctcc
     1501 ggtcgcttct agtgcttgca caaccggaac cagcgcctgg ctgattaacg tttccattcg
     1561 ctgctgcggc gaatgtggtg ttgcgttttt atcttcacac acatcgaccc agaaacaggc
     1621 gacgcgtccg gtttcgtgaa actcagcatg gaaatgttcc ggcgacacat ctaatgcttt
     1681 ttcctgcgtc agtagcgcca gcattaatgg tggcaccatc aggccgatat accattgtgc
     1741 ccatagtgag atcagcggtt tgttctcgcg gatcatcgtt ggttggttgc gatagatatg
     1801 atcggaatag accgccagta gagaacttag cgcattcggt gatgaccatt gcgccagcgt
     1861 catggcgtta agcggagcgg gttcatccag gcggataaac tccagcaaat gttcacgatg
     1921 tttggcgatc atcgcccgta cggcttgtgc aagcccagca tcctgcggct ggagatgcgt
     1981 tcgccagatg acatcttcat agagcggtgc ggaacgatag gccataatcg tgatagtaat
     2041 ctaaatgata atgattgcta atcctatcga taggtttact cgatagcaag ggatttctct
     2101 ggcttgcaaa tgataaaaat tatcatatga tattggtttt cattatcaat gaaagagatg
     2161 aaaccatgtt gcaacgtacg ctgggcagtg gctggggagt gttgctgccg ggattgctga
     2221 ttgcagggct gatgtatgcg gatttatcgc cggatcagtg gcggattgtc attctgatgg
     2281 gattagtatt gacgccggtg atgctgtatc acaaacagtt gcggcattac attttgctac
     2341 catcgtgcct ggcgcttatt gctggcatca tgctgatgat aatgaatttg aatcagggat
     2401 gacaaaacag ggaagaaaca agaaaggaag taaagataa
//
LOCUS       DADLTF010000283         2343 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.288, whole genome shotgun sequence.
ACCESSION   DADLTF010000283 DADLTF010000000
VERSION     DADLTF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.288"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..122
                     /locus_tag="JN749_004199"
     CDS             <1..122
                     /locus_tag="JN749_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370367.1"
                     /translation="QPKPPAPPAWAMMPPSNSLQLLDETFSVSGTESSATRQH"
     gene            complement(255..767)
                     /locus_tag="JN749_004200"
     CDS             complement(255..767)
                     /locus_tag="JN749_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HAY7370368.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            962..1726
                     /locus_tag="JN749_004201"
                     /pseudo
     CDS             962..1726
                     /locus_tag="JN749_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1677..>2343
                     /locus_tag="JN749_004202"
     CDS             1677..>2343
                     /locus_tag="JN749_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HAY7370369.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          664 a          522 c          592 g          565 t
ORIGIN      
        1 ctcagccgaa acctccagcg cctccggcat gggcaatgat gccaccgtcg aactctctcc
       61 agttgctgga cgaaacgttc tcggtatccg ggacggaatc atcagcgacc agacagcatt
      121 gagaatgctt caggagtaca tcaggatcca atgccttggg gggtagcggt aattttactc
      181 attatccttc aaatcagatt ctgttgtcag aggaatgggg gaagctggat tcgcaagagc
      241 tttttatggg cagtttagct aaaaaaaggc aacacgaagc taaaactacc aacataaagt
      301 gctgaccagc tagcagctga aatcagactc actgtataca ctttcactgg atgaagttgc
      361 aacattcctg ccaccaaagg agcaatgtaa cgcagcacgg cgataaagcg cgaagtgaaa
      421 agtattgaca cagagttatt ttgcaactga aggcgaaccc gttctattgt attggaacgg
      481 ctcgatatta tacatgcaag ttgagggata tgcccaatta ttataccaag atggtaatta
      541 acgatagtac cgcaccatgc gccactcatg accgtgattc cagcttccca tggtgaaaga
      601 gtcgtcttgc tgactgtaat tacagtggcc atcataattg atgctggtgg caatatcgcc
      661 gaaatgagta aagtggattt tgtgaacgct attataaaaa gaagccccca taggcgatat
      721 ggatgaagta caaaatagtt catcaaagca tttatccact ccatcaatgt ctttccttct
      781 gtgaattttt ctttgctatt taaccgtgat acgacttaat ttaggcttaa acaccttaca
      841 tttacaaggt actcctgtgg ggggccctgc cacggagcgt cgcaggagtg agtgatgagg
      901 aagaattaaa gcaacctatt gtgacaggca acgataacag taaatcacag gaagcataat
      961 catggcaaaa ccggactggg aggccatcga atcggcatac cgggccggag tccttagcct
     1021 ccgtgatata ggcgagaaat acggcgttac agaaggggct atcaggaaga gggccaaaaa
     1081 gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa aatggtacgc aaaaaaggaa
     1141 agtgcgtacc agcagaaagc ctgccattac tggccttaca caaaaaagta cgcaactaaa
     1201 aacagaatct acaccggata cgaaaccgat acgcggaatg cgtaccgatc ccccgactaa
     1261 cccattccaa cccggtaacc agcaggcatt aaaacacggt ggttatgccc gtcgccttct
     1321 gctcaaagat gaggtgatag aggacgctaa agcgttgacg ctcgaggacg aattatttcg
     1381 ccttcgtgct aacaaccttg tcgctgcaga gaatattggt cggtggttgg tgtcgctgga
     1441 agatgctaat ggggactagg aaaggaagat gctgattgaa aatatcagcg ccgccgagaa
     1501 agcaatgatg cgcaatacag ttcgtattga gtccatcgtt ggcacgcttg cgacggtagg
     1561 aaaaatattt gctgatacag cctaccgcaa ggccgccact gataaggtgt ctctggaggc
     1621 tgatcgtctt cgccgtgatg caggtattga tgatggcaat ggagagcgtg acctcaatga
     1681 cttctactct gacatccaaa ccgacgctga atccggcttt acgtagtttc tggactatgc
     1741 gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc aaaatcatgg gatgccgctg
     1801 gcattgccat atttctgtcg aataaataca ccctgcgttt ttgttgtgcc cgtcagatcc
     1861 agaataaaat cgaagagtcg gtgtataccc tgctcaaaat tcagatagac aggtttggtc
     1921 tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa ccgggttact ggctcggaat
     1981 ttgtttttta tggattatgg cgcaacatcg aagaaattaa gtcactggag gggatcgatg
     2041 tgttgtggct ggaagaagcc cacgcactga cggaatacca gtggaaaatt ctggagccaa
     2101 cgatccgtaa agagggttcg gaatgctggt tcatattcaa ccccggactt gttactgatt
     2161 tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac tctcatccgc aaaattaact
     2221 atgacgaaaa tccgtttctg tctgacacca tgcttaaggt tatcgacgcg gcgcgacgcc
     2281 gtgatccgga tggttttaaa catgtgtatg agggcgttcc ggagtctgat gatgatgcgg
     2341 caa
//
LOCUS       DADLTF010000284         2300 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.289, whole genome shotgun sequence.
ACCESSION   DADLTF010000284 DADLTF010000000
VERSION     DADLTF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.289"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..785)
                     /locus_tag="JN749_004203"
     CDS             complement(<1..785)
                     /locus_tag="JN749_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HAY7370370.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMS"
     gene            1133..2005
                     /gene="rluF"
                     /locus_tag="JN749_004204"
     CDS             1133..2005
                     /gene="rluF"
                     /locus_tag="JN749_004204"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HAY7370371.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2138..>2300)
                     /locus_tag="JN749_004205"
     CDS             complement(2138..>2300)
                     /locus_tag="JN749_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HAY7370372.1"
                     /translation="ETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWS
                     ANTSTRGRR"
BASE COUNT          562 a          588 c          576 g          574 t
ORIGIN      
        1 tcgctcatat tcgtttcaac cattgcgccg tgaatatcaa aaaagcgcga gcaaatatcg
       61 ccagcaacct ggcgggcatt caggtcgtca ctctcttcac caccataaaa cgcatgccag
      121 ttagcgccgt cgcgaatggc cggtgagcca attcccacca gggcgacatc cagattatcc
      181 cagtaggcag agatggtttt aaagtgctga gactgcatga tcccattacg aattaatggg
      241 ttatccagca gagccggaaa atccgcgaga tgcgattccc ctttcagctt cgctgccgcg
      301 ctgtaggtta atgtgtttac atgatagcgg ctttcgagtt taccggacgg tccgccaata
      361 attggtacgc agattaactg ccgcgattgc cccgcctgcg gcaagttttc aaccagtgcg
      421 ctcaccgcgc gaccccagga aaaaccgaca atatcgccag gctccagcaa gcgatccagc
      481 agttgcgcgc cgtgtaaccc catcatcgcc agttgagtct cttcatcctc atcatttccc
      541 gacaccacca caacgtcttt caggccaaac ttttgcttca gttgctgctc cagccagaga
      601 ttttcgttgt agtcatagtt gatggcgatg gtgaccattc cctgatcgcg gcctcgttta
      661 agcaagcggc tgatggtggt gcggtaaata cccagttcgc gcgcgatttg cgcctgcgtc
      721 atatcctgtt cgtaatagag ttgggcaatc ttcacaatca aacggatatc gtcactgttt
      781 tccattgccg cctcctggtt ctgcacattt gtgcaaatac gctttctcac cgcttgatct
      841 gatataaccc catcatgagg gcctttcaaa gcgatttttc actctttgca tggggagaaa
      901 tagcgctttc tcttgcacat ttgtttctct gcgtagtagt catcagcaca ggtgtgcagc
      961 gattcgtaat atgttgatta tccttatttt gccaggcaaa tttgcatcgt acttccggag
     1021 acacttttgt gataaaaggc gtggcagatc acatttaatt tcgacgttgc cgcataaccc
     1081 tgtttttccc cgctaagagg tacggtatat tccgcgtttt acaggagaat ttatgctgcc
     1141 cgactcatca gtccgtttaa ataaatacat cagcgaaagc ggaatttgct cacgccgcga
     1201 agcggatcgc tatatcgagc aaggcaatgt gttccttaat ggcaagcgag ccaccattgg
     1261 cgatcaggtg aaacccggcg acattgtgaa agtaaacggt cagttgattg agcctcggga
     1321 agccgaagat ttggtactta tcgccctgaa caagcccgtc ggtattgtca gcaccaccga
     1381 agatggcgag cgcgataaca ttgtcgattt cgttaaccac agcaaacgcg tgttcccgat
     1441 tggccgcctg gataaagact cccaggggct gattttcctc accaatcacg gcgatctggt
     1501 gaataagatc ctccgtgcgg gtaacgatca tgagaaagag tatctggtga cggtcgataa
     1561 accgattacc gatgaattta ttcgtggtat gggggcgggg gtgccgatcc tcgggacggt
     1621 gacgaaaaag tgcaaagtca aaaaagaagc gccgtttgtc ttccgcatta ccctagtgca
     1681 ggggctgaac cgtcagatcc ggcgcatgtg cgagcatttc ggctatgaag tgaaaaagct
     1741 ggaacgtacg cgcattatga acgtcagctt aagcggcatt ccgctggggg agtggcgcga
     1801 tttaaccgac gatgagttaa tcgacctctt taagctcatc gaaaattcct cttccgaggc
     1861 aaaacctaaa gcgaaggcca aaccgaaaac agcgggcatc aaacgtccag tcgttaagat
     1921 ggaaaaaacg gcggaaaaag gcggtcgccc ggcgtccaac ggtaagcgtt ttacctcgcc
     1981 ggggcgtaaa aagaagggac gctgattaac gcctcagact gatgaaaacg tccaaaaaga
     2041 gctcggacag tcccctcgcc ccttcgggga gagggttagg gtgaggggaa gattgtgtcc
     2101 acacagacga gtgaaaatct caggcgttga ttgttcatta gcgcctgccg cgcgtcgacg
     2161 tatttgctga ccagctaaat gacgcttcag gatccggttt ataagcctgc tttttcttca
     2221 attggcgggc ttttttcgct tcagcctcac gctcgaccat taatttatcg atgtactctt
     2281 tcttaatgct gttggtttcg
//
LOCUS       DADLTF010000285         2280 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.290, whole genome shotgun sequence.
ACCESSION   DADLTF010000285 DADLTF010000000
VERSION     DADLTF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.290"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="JN749_004206"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="JN749_004206"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAY7370373.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          578 a          564 c          671 g          467 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacgctt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagtc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgctatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact cagcacatgg
//
LOCUS       DADLTF010000286         2231 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.291, whole genome shotgun sequence.
ACCESSION   DADLTF010000286 DADLTF010000000
VERSION     DADLTF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.291"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(404..601)
                     /locus_tag="JN749_004207"
     CDS             complement(404..601)
                     /locus_tag="JN749_004207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370374.1"
                     /translation="MLGDNPNIDVVAMVPIIIVDMKIEVTLKLSDITVGNIMSAVSIM
                     AKDTLNTESVEKRLLSEKCMA"
     gene            1447..>2231
                     /locus_tag="JN749_004208"
     CDS             1447..>2231
                     /locus_tag="JN749_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HAY7370375.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFAFTILIINTINSGETI
                     DYIYSHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVG"
BASE COUNT          714 a          399 c          359 g          759 t
ORIGIN      
        1 tatcatcatt caatgaacag atagctgtag tcaatgtatt attatgcacc acactgttaa
       61 ttttttgcaa aacaaacaat tacaatacaa aaccattatt aaatgcgatg gggatttcac
      121 tattagttat tatttatatt gcaacttgcc caggaaataa agtcaggtat tatgcagaga
      181 tagaacattg gtttaaagag tatggtaatt ttaatataat ccaaagatct atgcttggac
      241 ttaacttata tgcagatatg ttattttctg taaaatcaat tatcccagca ttactagctt
      301 tttccagtac tattatatgc aagaaaaaaa caaaaatact ccctctaatt tcttgctgta
      361 tcttgatcac actatatatt atacatacac cgccagttat ttttcaagcc atacattttt
      421 ctgaaagcaa tcttttctcg acactctccg tgtttagggt gtcttttgcc atgatactta
      481 cagcactcat tatatttcca accgtaatat cacttaattt taatgtcact tcaattttca
      541 tatccacaat gattattgga accattgcca caacatctat gttaggatta tcaccaagca
      601 tatatgcttc tggtaataga atttatttta ttccatattt gcttcttata acagcaattg
      661 ttgtttcaac cccaatcgca ataaataaca tagtagcaaa ttttgccaag agccattata
      721 aaatttgaat taaatttaaa agccacctcc tttttttggg gggggcttag aaataaaaac
      781 aaatttaatt ggacttttat tggttataac acattcctct atactgctca tcacccgact
      841 atactaggat tttcgtcatt ttttgctgaa ccgctctcat gaaatcctga caacaaagtc
      901 atgtttcacg tcaggatact tcttctggca tggaaatata ccgtgtgcag ctgatgcata
      961 tttatatttc aatataatac gatattgaat ggcctacacc actggtagtt caggccaaat
     1021 gacatccgac ggtttcacga tgaactacgg caaaagaata tcagagccct cattcctccc
     1081 cgaaagggag cgggctctgg tctggtgagt atgcagatcg taaaggctat ggccatggtg
     1141 cgagagtggc agctatgcca gaaagagtgt gtattgcctg agactctaac ctgatgcaga
     1201 gacgattgtc ttgaacctga tttattcaac aaagccgctg gcaggatgtt acgaaagtta
     1261 tcgcggccgc aggctgattt gatatcgata ctatcggtgg tactgcggaa gtaaatgcct
     1321 atatagtgcg actttgaaaa ataataaatt aatattgaac tcgttgatta ataatcataa
     1381 actttcaggc attcatattt gcaatgagtg tttgaaaatc acacaaaact ggagagccta
     1441 ctttaaatgt ataataacaa aatcgacaac aacagaaaaa caaggcatga ttgggttgat
     1501 gctttaaaat ttctcggtat ttttgctata tatttaggtc accttggttt gggggctggc
     1561 aaattgtatc cttttgtttt tagttatcac gtcccactat tctttttcgc tgctggtttt
     1621 tttactatca aaaaaaatga cttatctatt ttcgattata tcaagtcaaa gttttacaga
     1681 ttaatgattc catatttcac ttttgcattt actatattaa taataaacac aattaactca
     1741 ggtgagacga ttgattatat atatagccac atttatgata taatatatgg tgttagaaac
     1801 aaccaatttg taggaacaat atggtttata aactgccttt ttgttataat agcaattgat
     1861 gcaatcttta aagaaatagt caagaacaat atagtgattt taatcatatc actattatcg
     1921 ttcatgttgt cacaaacagt gctcaatcat aacccattac tagatcctca atggttttgg
     1981 aacatagact ccgctatggc atactggtgg ttattgcctc tcggtagatg tatgttttta
     2041 gaattaacac gtgacaggtt ttttggaaaa tctaaaattg gtttcattgt cttctcaatt
     2101 accgcgataa tgagtgcgta tcaactcctt aaccagaaac ctttgctttt taagattatc
     2161 tcaatattca atgcagacat aatatcaagt agttatattc aagctattaa tactattatc
     2221 accacggtag g
//
LOCUS       DADLTF010000287         2205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.292, whole genome shotgun sequence.
ACCESSION   DADLTF010000287 DADLTF010000000
VERSION     DADLTF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.292"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..413
                     /locus_tag="JN749_004209"
     CDS             <1..413
                     /locus_tag="JN749_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAY7370376.1"
                     /translation="ERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLAL
                     EGITSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLIFGNPVRGYPSLLVSILFLGG
                     VQLIGIGVLGEYIGRIYIESKHRPKYIIKNEKQK"
     gene            410..1672
                     /locus_tag="JN749_004210"
                     /pseudo
     CDS             410..1672
                     /locus_tag="JN749_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967842.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
     gene            complement(1796..2203)
                     /locus_tag="JN749_004211"
     CDS             complement(1796..2203)
                     /locus_tag="JN749_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7370377.1"
                     /translation="MSYFYSASTNGFYSTEFHGTNIPDDAVEISESEWKTLINSQSVT
                     KMITCGEDGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQDAVDLGMATDD
                     ETKLLLAWKKYRVLLMRVDIKNTEWPKKPEENK"
BASE COUNT          646 a          429 c          377 g          753 t
ORIGIN      
        1 ctgagcgaaa tcttttcatg aaaggcatac tgagctgggt gggtggtcag acggatgtcg
       61 ttgaatatgt acgcgcagag cgtgttgctg gcatctcaaa atttaatggc tggaaattat
      121 ggaatctggc actggaaggc atcacaagtt tttcaacctt tcctcttcgc gtatggactt
      181 atataggctt gtttgttgca agcatttcgt ttttatatgg tgcatggatg attatagaca
      241 cccttatctt tggcaaccca gttcgcggat atccctccct gcttgtatca atacttttct
      301 tgggtggagt gcagctgatc gggattggtg ttctcggtga atatataggc agaatctata
      361 ttgaaagcaa acatcgtccc aaatacatca tcaaaaatga gaaacaaaaa tgaaaagcct
      421 taaaacatca tatgtaaaaa aactattttt cttaattgct gtatttttca ttgcggttat
      481 tttgatgtat ttgcgccgcc ctgatataat cagaacaccg cagttttggg cagaagatgg
      541 tcatgtatgg tatgctatgg catacaataa tggcatattc acatcaatga tattccctca
      601 aaatggatat tatcaaacca tatcaaacca tatcaaaact tattgcctcc atatcattaa
      661 attttaattt aatgtatgcc cctttaattt ttaacatctc cgcaattatt gttagggcat
      721 tgcttgtatc tttcatactc tctggaagat ttagttacgt aaacataaca ccaagaatat
      781 ttactgcttt gtttattata ataatgccgg aagtatcaga agtgcacgcc aatgttacta
      841 atgattattg gtatttatca ctatatttac ttcttgttat gttagctcca aagcctacaa
      901 acaattatca aaaagcacac gattatatag cgataatcat atgtggacta agtggtccat
      961 ttatagtatt catggctcca atggtaggga taaatatact tatacaaaaa cgcttatcta
     1021 caaaaaaata accactcacg aatacgtttt cattgccatt tgtttgattc aatttatttc
     1081 tattattatg acatcctctg agaccagagt tgatatggtt cttggagcta atttcgcaac
     1141 actttgcaaa atattaacaa caaaggtttt tctgggttta tgggctaatg gtgacttttt
     1201 atcaccttta tggaatcatg attcaatttg cattgcaatt acaatgattt gtctttccat
     1261 cactattttt acatttattg tttccaatta ccccatgcgc tcggcaatag ttttcgcaat
     1321 actaacatta acgttttcac tagcaaaacc aatgctttca tctacaagtg agcaatggcc
     1381 attattaatt catggcggag gaagatattc tgtcatacca acaatcatat ggtactctgt
     1441 attggtttac ttcttaaact atatttccca cttgcacttt aaattcctac catggatgtt
     1501 atatacaaca tcgttaatct catgtttata ttttttcaat cttaccccac tcccagatta
     1561 cgggtggaaa gagcaagtta aaaaatttga aagcttagca ccaggagaat cgtattcttt
     1621 caagttcaac cctgcaggat ggacgatgac attaataaaa aaacagcctt aatgttgaat
     1681 ggtagttact taatgttatt gatctaagtt ttgcatcaat aacccccccc gaaacttagg
     1741 ggggggcatc tgcagttata attaacggcc tctttcttca tccgataacg cccccttatt
     1801 tattttcctc tggttttttc ggccactctg tattttttat atcaacacgc atcaatagca
     1861 cccgatattt tttccatgcc agtaatagtt tcgtttcatc atctgttgcc atacctaaat
     1921 caaccgcatc ctgaagcgga gctattacat tcgttgcctc tgctatcagt gcagatttct
     1981 tttcatcatt tattaaggct aattgttctg gtgttggaga aggacggtca acaatgacag
     2041 ggtgaccgtc ctcaccacag gtaatcattt ttgtgacact ctgtgagtta atcagtgttt
     2101 tccactctga ttccgagatt tccactgcat catcaggaat attggtgccg tgaaattcag
     2161 tcgaataaaa tccgtttgtc gatgcagaat aaaaatatga cattt
//
LOCUS       DADLTF010000288         2152 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.293, whole genome shotgun sequence.
ACCESSION   DADLTF010000288 DADLTF010000000
VERSION     DADLTF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.293"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            83..463
                     /locus_tag="JN749_004212"
     CDS             83..463
                     /locus_tag="JN749_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HAY7370378.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(504..1454)
                     /gene="corA"
                     /locus_tag="JN749_004213"
     CDS             complement(504..1454)
                     /gene="corA"
                     /locus_tag="JN749_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HAY7370379.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1824..>2152
                     /locus_tag="JN749_004214"
     CDS             1824..>2152
                     /locus_tag="JN749_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370380.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGDIYDESYAPLG
                     LYIENGQR"
BASE COUNT          580 a          506 c          511 g          555 t
ORIGIN      
        1 tttttgattt gttatttttc cactttcagt atgctaatgc ttgcacctac tgattcatca
       61 aaacaatagt tatggagtat ttatgagtaa ggaatatatg aacgatgatt cattaagtga
      121 aaaatggaaa taccgattta atttttatga tcaacatggc tttcctggat tctggggagc
      181 aacaccagaa tataaagcag catttaaagc attaaaagta agacaacgat taactattca
      241 aatgaatttc attgctttct tctgttcctg gatctattta tttgttctgg gcttatggaa
      301 aaaagcaatt atagttctat tacttggcat tctatctcta tttgttggtg cattaattgg
      361 cgttaacatt ctgggaattg ccgtagcagc ctatgttgct gttaacacga acaaatggtt
      421 ttatgagaaa gaggtaaaag ggttaaatac ctggagcctt tgaataacag gtggcttagc
      481 caggctaagc caccgccttc tttttacaac cagttcttcc gcttaaagta cagatacggt
      541 gccaggcccg cgaggatcat aaagataatc gcgccagggt agccgaaact ccacttcagt
      601 tctggcataa actcaaagtt catcccgtag ctggaagcaa ccagcgttgg cggcaggaac
      661 acgacggata ccaccgagaa aattttgatg atgcggttct gctcgatgtt gataaaaccc
      721 attgccgcct gcatcaggaa gttcaccttc tggaacaggg attcgttatg cggcagcagg
      781 gattcgatat cgcgcaggat ttcacgcgcc tgctccagtt gcccacccgg taaacgcgct
      841 ttacgcacca ggaagttgag agcgcgctgg gtatccatca gacacaggcg aactttccag
      901 ccgatatctt ccagttccgc cagcgtggag agcgcctcgt cgtactcatc gccctgatgc
      961 ccttccataa tcacccggct caactgctcc aggtcgctat agatattttc aatttcatct
     1021 gccaactgtt cgattttggt ttcgaacaga tccagcagca actcgtaggc gttaccgtct
     1081 accatcgact ggctacgggc acgcatacga tacagacgaa aagcgggcag ttcacgctca
     1141 cgcagagtaa acagacgacc atcacggatg gtaaatgcca cagtggagtt accggcgtga
     1201 tcttccgcat cttcaaagaa gaagaaggag tgaatatgca ggccgtcgtc gtcttcaaag
     1261 aaacgtgccg atgcttcgat gtcttccagt tccgggcggg ttgccaggct ctggccaagt
     1321 tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga caagatcaat ccatactgca
     1381 tttacaaggg gttgtgactc ttcgacttcc agccgggtca gtcggttatt ttccagttga
     1441 aatgcgctca gcatgaccgg gactcccaat gcttaaaata tcggacagtt cggtgggcac
     1501 acagaaacaa attgggtttc agaccattaa acagcctgac tcagcgcgac gggaaaaatt
     1561 gaggtcgctg acaaccgcta aggctatcag caaaaaggga tagccttagg agttgatcct
     1621 ggatgacagg atagtgagcc agtatctact gggtgtgtcc aaggcgaatg tcctcttaga
     1681 gtgatcgtgc gcgcatgtta cgccagcaaa attttgccgt caacacgcaa cagaacacca
     1741 cagaacaata gttgaacttc gcgctttttg gtactcgtca aggtcgctaa caggataaaa
     1801 aaatcccaca tccaggacct ataatggcgt atcggctact cattggtaaa ggaatgatca
     1861 ccttgaatct caaacggatt tttctcaccc tcaccttact tcccttgttt gctgttgccg
     1921 ctgatgattg cgcactctca gatccgacgc tgaccgtaca ggcgtatacc gttaatcctc
     1981 aaacagagcg ggtgaaaatg tactggcaaa aagccaatgg cgaagcgtgg ggaacgttac
     2041 atgctctgct ggcggatatt aatagtcagg gtcaggtgca gatggcgatg aacggcgaca
     2101 tctatgatga aagctatgcg ccgctcggtt tgtacatcga aaacggtcag cg
//
LOCUS       DADLTF010000289         2151 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.294, whole genome shotgun sequence.
ACCESSION   DADLTF010000289 DADLTF010000000
VERSION     DADLTF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.294"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JN749_004215"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="JN749_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HAY7370381.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="JN749_004216"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="JN749_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="JN749_004217"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="JN749_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="JN749_004218"
     CDS             1905..>2151
                     /locus_tag="JN749_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370382.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          634 a          395 c          490 g          632 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaacacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt accgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagta ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DADLTF010000290         2135 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.295, whole genome shotgun sequence.
ACCESSION   DADLTF010000290 DADLTF010000000
VERSION     DADLTF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.295"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>2135
                     /locus_tag="JN749_004219"
     rRNA            <1..>2135
                     /locus_tag="JN749_004219"
                     /product="23S ribosomal RNA"
BASE COUNT          587 a          467 c          654 g          427 t
ORIGIN      
        1 gacgtgctaa tctgcgataa gcgtcggtaa ggtgatatga accgttataa ccggcgattt
       61 ccgaatgggg aaacccagtg tgattcgtca cactatcatt aactgaatcc ataggttaat
      121 gaggcgaacc gggggaactg aaacatctaa gtaccccgag gaaaagaaat caaccgagat
      181 tcccccagta gcggcgagcg aacggggagc agcccagagc ctgaatcagt gtgtgtgtta
      241 gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc cccgtacaca aaaatgcaca
      301 tactgtgagc tcgatgagta gggcgggaca cgtggtatcc tgtctgaata tggggggacc
      361 atcctccaag gctaaatact cctgactgac cgatagtgaa ccagtaccgt gagggaaagg
      421 cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa accgtgtacg tacaagcagt
      481 gggagcacgc ttaggcgtgt gactgcgtac cttttgtata atgggtcagc gacttatatt
      541 ctgtagcaag gttaaccgaa taggggagcc gaagggaaac cgagtcttaa ctgggcgtta
      601 agttgcaggg tatagacccg aaacccggtg atctagccat gggcaggttg aaggttgggt
      661 aacactaact ggaggaccga accgactaat gttgaaaaat tagcggatga cttgtggctg
      721 ggggtgaaag gccaatcaaa ccgggagata gctggttctc cccgaaagct atttaggtag
      781 cgcctcgtga attcatctcc gggggtagag cactgtttcg gcaagggggt catcccgact
      841 taccaacccg atgcaaactg cgaataccgg agaatgttat cacgggagac acacggcggg
      901 tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc cagctaaggt cccaaagtca
      961 tggttaagtg ggaaacgatg tgggaaggcc cagacagcca ggatgttggc ttagaagcag
     1021 ccatcattta aagaaagcgt aatagctcac tggtcgagtc ggcctgcgcg gaagatgtaa
     1081 cggggctaaa ccatgcaccg aagctgcggc agcgacgctt atgcgttgtt gggtagggga
     1141 gcgttctgta agcctgtgaa ggtgtactgt gaggtatgct ggaggtatca gaagtgcgaa
     1201 tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga ccaagggttc
     1261 ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag
     1321 tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg ggacggagaa
     1381 ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt tttccaggca
     1441 aatccggaaa atcaaggccg aggcgtgatg acgaggcact acggtgctga agcaacaaat
     1501 gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac cccaaaccga
     1561 cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga aggaactagg
     1621 caaaatggtg ccgtaacttc gggagaaggc acgctgatac gtaggtgaag cgacttgctc
     1681 gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa aaacacagca
     1741 ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgcc ggaaggttaa
     1801 ttgatggggt tagcgcaagc gaagctcttg atcgaagccc cggtaaacgg cggccgtaac
     1861 tataacggtc ctaaggtagc gaaattcctt gtcgggtaag ttccgacctg cacgaatggc
     1921 gtaatgatgg ccaggctgtc tccacccgag actcagtgaa attgaactcg ctgtgaagat
     1981 gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct ttactatagc ttgacactga
     2041 acattgagcc ttgatgtgta ggataggtgg gaggctttga agtgtggacg ccagtctgca
     2101 tggagccgac cttgaaatac caccctttaa tgttt
//
LOCUS       DADLTF010000291         2128 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.296, whole genome shotgun sequence.
ACCESSION   DADLTF010000291 DADLTF010000000
VERSION     DADLTF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.296"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..161
                     /locus_tag="JN749_004220"
     CDS             <1..161
                     /locus_tag="JN749_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001436458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAY7370383.1"
                     /translation="CRYEKASIILTSNKGVADWGEMFGDHVLATAILDRLLHAEYQRR
                     ELPVKRET"
     gene            655..1233
                     /locus_tag="JN749_004221"
     CDS             655..1233
                     /locus_tag="JN749_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370384.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="JN749_004222"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="JN749_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2128
                     /locus_tag="JN749_004223"
                     /pseudo
     CDS             1584..2128
                     /locus_tag="JN749_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          688 a          390 c          523 g          527 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctacggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaatggt agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctactggca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa agaacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttgtagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagctcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttccta ttgaaagtcg tcgtcagaca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacga ctggcagaga tggctgcggc
     1921 caacagtgac gtccccatga tgaagaatgt ggctaaaacg atagggaaaa ggttgtacgg
     1981 tatcctgaac gccatgcgcc atggcgtatc aaacggaaat gcggaagcac tgaacagcaa
     2041 gatcaggctg ctgagaataa aagccaaggg ctaccgcaac cgggagcgct ttaagctggg
     2101 agtgatgttc cactatggga aactgaat
//
LOCUS       DADLTF010000292         2079 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.297, whole genome shotgun sequence.
ACCESSION   DADLTF010000292 DADLTF010000000
VERSION     DADLTF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.297"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(197..430)
                     /locus_tag="JN749_004224"
                     /pseudo
     CDS             complement(197..430)
                     /locus_tag="JN749_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439239.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(526..2049)
                     /locus_tag="JN749_004225"
     CDS             complement(526..2049)
                     /locus_tag="JN749_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7370385.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          622 a          460 c          443 g          554 t
ORIGIN      
        1 cgacatcaaa attattaata gttgccatgg taattatttc ggtgatatat aaaaacaact
       61 gtgataacac caaggttgac aactatttgc gcaataattc ctttccttca atgatccagg
      121 cgttcactgc cagcaaccca ttcagccgca tactgacagt gagaaactga aagattattg
      181 ctgatccctg tggctgacga gtctttctct taaatcgatt aaccagattg atcgccagcc
      241 aaacaagggc attaagcaaa tttatcgtca caagcacttc tcccagcccg gtactgggtt
      301 ttgcaataag gatgaccagg agggtgatca gcataaatgc aatggaaaag atgatataga
      361 gagtgaaata atccagatta ttactattgt tcatgctact aatggcaatc ctgtcaaaac
      421 tttcaaacaa ttatagcaaa caaaataatg aacaatagta acaagaggca acgagctaat
      481 tagctgtcta taattacacc gcactatggc cttactgatc aattttcact caaccacaac
      541 cttcaccttc ggtttagaaa ggataatttt gaccaggtcg atgtatgtaa caggtttata
      601 gaaataataa ccctgctgaa atgtgatatt attttggttc agatagccaa gttgttcttt
      661 cgtttcgaca ccttcagcga cgatactgat cgaaagttta cgcgccagat ccaatacaca
      721 atccagaatt cgggttgatt ctgggtctgc gtttacgcgg ccaacgaaac tgtgatcaat
      781 tttgatataa tcaatatgca ggtcatgaag ataagaaagc cctgagtagc cagtaccgaa
      841 atcatccagc gcgatgacaa aaccattttc atgcagaatg ttcaaccgct gaaccagact
      901 ttcatcaaca ttcaatggct cacgctcggt gacttccaga acaaggttta aatcgcggcg
      961 ggtaaaactg tcacggaaat ttaaacactc gtcgacaaac gtcggcgaaa taatatgcga
     1021 agcgctaaaa ttaattccaa tatgaaaacc ttccggcagt ttactcgcga tagcgttcat
     1081 ctgtctggca acctgattca tcaggctttg tgtaagcgga acgattaatc cggatttttc
     1141 agcaagtgga ataaatgccg cgggtgaaat atatccaccg tgaggttgtt tccagcgggc
     1201 taacacctca actccccgca atgtcccttc ccgaccattt accacaggtt ggtaaaaagg
     1261 gacgatctcc ccattattta ttgcccgtcg caggatctct tcaggcgttg cgcttttatt
     1321 gaaatatcta tcaagcagga aggcagcggc gcaggcgatt aacagaataa atattaaaat
     1381 cccaaagcct ttatcgataa gtctgtagaa gctaaagagt ggcggcgggt taaagataat
     1441 agtgaaatga tattttgtgg agtgaacctt ctcggtatag ggataatgtc cattaagcgt
     1501 catcacatca cccgttggtc caataatgtc atccgccacg cgtaatacat accttacccc
     1561 tttcaagggt acattaagcg ccccgcgaat atgttgatca cttatcgtaa ccaaaatgcg
     1621 cgtatctgca aattggctct gatagagcag gataggtaat ctattaacgg tgtcgattgc
     1681 cggagccaac agtaaattac tgtccgggac aacaggaata cgagacagca ggacccgatt
     1741 cccaggcagg gatgtacacc agactattcc ctgtttgaga atgatgattg tgcgcaggtg
     1801 aggtttcaga gctgcttcag tgccaagctg atattgctcc tcgaggtcgc attccttccc
     1861 ggcaatgtta accgctgttt gagtcgcctg tgacgcttct tcaagtatat gattgatatt
     1921 gttggcggca tatcttgctc ccgccaggta ctctgctttt gcggaatacc agagctggat
     1981 gttcaaaata gcgacaccgg taataaacaa gcatatcgca acgaggatgg gtatgagtgt
     2041 attgcgcatt tgcttgtccc ggccaaaggg tttaataca
//
LOCUS       DADLTF010000293         2074 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.298, whole genome shotgun sequence.
ACCESSION   DADLTF010000293 DADLTF010000000
VERSION     DADLTF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.298"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..606
                     /locus_tag="JN749_004226"
     CDS             <1..606
                     /locus_tag="JN749_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7370386.1"
                     /translation="AKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEA
                     GTVFIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAM
                     VFNGLKLMKNGYGEQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYA
                     QAIMGHSSGSITYDRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            725..1058
                     /locus_tag="JN749_004227"
                     /pseudo
     CDS             725..1058
                     /locus_tag="JN749_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708644.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          581 a          459 c          506 g          528 t
ORIGIN      
        1 gctaaagcgt actcgcagaa gacctcaggt aagccatacc attattacgt caccgctctg
       61 gctgcgatta caggtgcgag acttaacgag gtggcccaac ttcaggttaa agatgtcagg
      121 accactgagg ctgggaccgt ttttatccat atcaacgaag acgatagcag cctgccgggt
      181 aagagtatca agaacgccca cagtgaccgc tgtgtcccgt tggtagatgg tgcttatggt
      241 tttgtattgg ctgactttat gtcactcgtg gaagaccgta gaaagactga gggtgataat
      301 gctatggtgt tcaatggcct caagctaatg aagaacggct acggtgagca ggttagcaag
      361 tggtttaacc gaacgttgct gcccaaagtc ctcgctgacc gtagcggctt agcttttcac
      421 tcgtttagac atacagttgc gacccagtta aaacaacatg gggtcgagtt agcgtatgct
      481 caggcgatta tgggtcatag ctcagggtct ataacttacg accgttacgc gaaagaggtc
      541 gaggtggaaa cgctgaaaga aaaattagcg gagtcattgt cagtaaaaaa aatagacgga
      601 aagtaatcgc atagggatag cggaatgtga ggacgtaatg gtcaaagaat ttttagttag
      661 caaagaaaat aagccttcat atgaatgtat gaatactgct gaaacttgtt tggggctggc
      721 tggaatggct tcggaagatt ggcaacgtga gcatcattgc cgcacaggta taatatttac
      781 tgcttttgct attgaggcaa tgttcatctt ctatagaaaa caggttgacc ccggctacga
      841 taagacgcag aaagagtgca gaaagacaat gcacaagaat acgttaaaat tgtgcggtat
      901 aaacaattat atggggacaa aaccttacca aattatcaaa gagtgtcttg aggtaagaga
      961 cgctattgct cacggcgatt cgtatacttc gagctttaat ttctcggcgg atcatttgga
     1021 taaccaagac gatattgcaa aaagtgtcct ggcggtaaac tctgaacaat ttcggggatt
     1081 aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc atgagatttc
     1141 tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca gaagtctgat
     1201 gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga taatacacct
     1261 cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa gtccccacct
     1321 taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg tagcggaccg
     1381 attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac cattcctttt
     1441 aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc gactttaagt
     1501 tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt actgaggtct
     1561 gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg tctgaggttg
     1621 gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg ttatggattg
     1681 tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg ccaacccgaa
     1741 gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg aagtactgaa
     1801 cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc actataggga
     1861 ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt ccacgctagc
     1921 aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt ctttggtctc
     1981 gtgacttaag tctatcacca cacacgctcg gacatgctgt cgaaccttca tctctatggt
     2041 catcgcagac cactaacccc aattacctca agtc
//
LOCUS       DADLTF010000294         2050 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.299, whole genome shotgun sequence.
ACCESSION   DADLTF010000294 DADLTF010000000
VERSION     DADLTF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.299"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..169
                     /locus_tag="JN749_004228"
     CDS             <1..169
                     /locus_tag="JN749_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HAY7370387.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            154..792
                     /locus_tag="JN749_004229"
     CDS             154..792
                     /locus_tag="JN749_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HAY7370388.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1020..1421
                     /locus_tag="JN749_004230"
     CDS             1020..1421
                     /locus_tag="JN749_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370389.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSSGGGR"
     gene            1432..1906
                     /locus_tag="JN749_004231"
                     /pseudo
     CDS             1432..1906
                     /locus_tag="JN749_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT          620 a          366 c          481 g          583 t
ORIGIN      
        1 gtccgctatt gccagtacac ctcacccgtc ctggcgacgt atctgtaaga cactcataaa
       61 gaatgatttt tggtgccgga ctctctcctt cagtccgaac aaaccccggc actatgaacg
      121 ttacctgcag cgtatgaagg aaaggaggaa ggaatggggg attctgtgac accggagata
      181 cttggcaaca tgattcgtca gtatttcagt caggtacgaa ctgaaaaaga gagcatacag
      241 gctctgaatc atctgcgcag agtgcttcat gaggtcagtc cttttgcaca ggagcctgtg
      301 gactgtgtgt tgtgggtgaa agcggatgag gtggtggcca acgattacaa ccctaatgtc
      361 atggcactag gtgagaaaaa actactgaaa cagtctctgg agaaagatgg tttcacacaa
      421 ccggtggtgg tgtcagagga gaagaatcat tatctggtgg tggatggttt tcatcgtcag
      481 ttactggggc gggaagcaga tacaggaaaa cgcctgaggg gctggttgcc tgtagcctgt
      541 attaatccgg agagaaaggg acaggccgca cgtattgccg cgaccattcg acataaccgg
      601 gccaggggga aacaccagat cactttaatg tctgatattg tcagggatct ttcacgtctg
      661 ggctggacga atgaacgtat tggtacagag ctgggaatgg accaggatga agtgctgagg
      721 ctgaaacaga ttagcgggct gacagagttg tttcaggaag aggatttcag ctcggcttgg
      781 acggttcgtt gatgttgtta aatatgggcg gtatttctga aagttgatac tttgggtaga
      841 aaaaatgata ccgaaaacta tggaggatgt gcttgtcagg tgactggaca ccataggtct
      901 taggctttga taacgggata aaatgagcgg cttttctcat accatcaggt gtgatgatgg
      961 cgcagattta caaaatctga ttgattctaa agatgtataa gtaaaaatca agctttgata
     1021 tgaaaaaaca gatatttatt aataataaac ctcctgtagt cccttactca gggacgcacg
     1081 cgaaaatatt taaatacatt gaaatccctc tgccgttttt ctactttatc tatacatcag
     1141 gcgaaccatt ccacatatct gtccaaaata ctgttattta tgtatcaaaa tacaatggta
     1201 tatttattaa taaattagtt cctttttctc ttttatttga cagggatata tctgttcttc
     1261 aaagaagaga tatttgtgtt gtgcgattta cgtcagagga aataagtgaa cataacgttt
     1321 tatttgatca tgacattgaa cgattaaaaa aaatttccaa agctcagctt atatctcccg
     1381 attatgtttt gattgatttt tctagtgggg gggggagatg aaccctatgc aatgtcctgg
     1441 ttaatatctc agtgtgctca tcagtgcact gataataaaa agacggagac agatgccatt
     1501 tacgataaag ttcggagttc ttatttgctt tcatgtattc tgaaaaaaaa caaaaatgtt
     1561 gggcttattc ttcatgcacc atcatttgtt tctgtgtctg aaaaaaatcg ccagaattgt
     1621 gatggcaaat tactcccgta actggagtaa ttccgagttg gccagtgcgg ttttaatgag
     1681 tgaatcttct ttaaaaagaa gaatgtacaa agaggtcggt tctataagta catttgtaca
     1741 taaaataaag cttacagaag ctatcaggaa attacgaagg acaaatactc ccatttctgt
     1801 catatcatct gagttaggtt acagttcacc atcgtatttt tctaaagttt tctttaaata
     1861 tttaaagaca taccctcaaa acataaggaa gaaaaatggt cggtaatgtc accaattagg
     1921 aggacttgca gagccgcttt tttacgatac tgagatcaaa cagacataat ctatgcggag
     1981 atttacagca gggcatggtt cacaaatctg acagcgatga attatcggca ctgcgggcag
     2041 aaaatgctcg
//
LOCUS       DADLTF010000295         2042 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.300, whole genome shotgun sequence.
ACCESSION   DADLTF010000295 DADLTF010000000
VERSION     DADLTF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.300"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(220..384)
                     /locus_tag="JN749_004232"
     CDS             complement(220..384)
                     /locus_tag="JN749_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370390.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="JN749_004233"
     CDS             1286..1570
                     /locus_tag="JN749_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAY7370391.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..>2042
                     /locus_tag="JN749_004234"
     CDS             1558..>2042
                     /locus_tag="JN749_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7370392.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          527 a          427 c          584 g          504 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggttccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 ggtgaagctg tacttgatga ctggttaaag cagaaggggc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtatcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 ta
//
LOCUS       DADLTF010000296         2032 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.301, whole genome shotgun sequence.
ACCESSION   DADLTF010000296 DADLTF010000000
VERSION     DADLTF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.301"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..362)
                     /locus_tag="JN749_004235"
     CDS             complement(<1..362)
                     /locus_tag="JN749_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7370393.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="JN749_004236"
     CDS             complement(429..1643)
                     /locus_tag="JN749_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HAY7370394.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          632 a          493 c          414 g          493 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcgattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DADLTF010000297         2018 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.302, whole genome shotgun sequence.
ACCESSION   DADLTF010000297 DADLTF010000000
VERSION     DADLTF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.302"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..188
                     /locus_tag="JN749_004237"
     CDS             <1..188
                     /locus_tag="JN749_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7370395.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JN749_004238"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="JN749_004238"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAY7370396.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..>2018
                     /locus_tag="JN749_004239"
     CDS             1156..>2018
                     /locus_tag="JN749_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7370397.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLALQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGT"
BASE COUNT          481 a          539 c          517 g          481 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aatcagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aaccggtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gctctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcac
//
LOCUS       DADLTF010000298         2009 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.303, whole genome shotgun sequence.
ACCESSION   DADLTF010000298 DADLTF010000000
VERSION     DADLTF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.303"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            320..573
                     /locus_tag="JN749_004240"
                     /pseudo
     CDS             320..573
                     /locus_tag="JN749_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-D"
     gene            730..>2009
                     /locus_tag="JN749_004241"
     CDS             730..>2009
                     /locus_tag="JN749_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7370398.1"
                     /translation="MRQLVISITEGLNMSLFTEPKEIERLPSEEIERLYPVLRYRVFI
                     SIFLGYMGYYFVRNTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSK
                     VFLSLGLFLSGLVNFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEH
                     RERITWATLWNLSHNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLI
                     YFIQVDRPVSVGLPPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCI
                     YGSFVYILRYGIVSWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLS
                     VRLFRNSKPLTNTLFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIG
                     LYSMELVPKEAAGAASGLSGTFSYIFGSIVATLG"
BASE COUNT          527 a          397 c          457 g          628 t
ORIGIN      
        1 tgcgacccag acctgattag gggggttggg gagcaatgga acagtaaacg ccgttaagaa
       61 gtcaatttaa atagccaaaa attaactagc caatttacat tgcaggttgt aatttttaac
      121 cacctttttt acgatagcca aattacctat acccatcccg gccctgccaa gccatacccg
      181 ctatcggcga tgaaaatgct tttgtgcgcg gtcggagtgg tcccgacgag ggttttaccc
      241 gaagtcgggg cgtatctccg cgttagcggg ccgtgagggc cgcttacgag cgtgtatgag
      301 agacgtacag tggttttgtc cgctcacacc agtacataca ggtacggaaa taaaaccagt
      361 cgaaatgccg gtaacgacga ttactcctgt ctctgatgcc ggtggactac aagattttat
      421 ttactggcgt cctgatgcgg acaggactgg tatggaacct gtttatgtga tacttaatga
      481 tcctctggat tccgggcgat ttacgcgtaa acaactaaat aaaaatatct taaacatgca
      541 agtaattttg gtattctcga tacaagaata aatagtagta actactatag ttgcaaataa
      601 tgttaaaaac atttctatga tcatctttac attttttatg attgacaatt gtttttggtt
      661 aaaaaaagac aactaatgag tgtgataatg caatactttg agtaattata taatgataag
      721 taaacaggtt tgcgacaatt ggttatcagt attacagaag gattaaatat gtcattattc
      781 acagaaccta aggaaattga aagattaccc tctgaagaga ttgaacgatt atatcccgtc
      841 ctgcgataca gggtctttat cagcattttc ctcggatata tggggtatta ttttgtccga
      901 aacaccacca gtgtgttatc cggtgtgctg cacatgtctg caaccgagat cggcattatc
      961 tcctgtgccg gttttttatc ctatggtata tcaaagtttg tttccggact tatttctgac
     1021 agaagtaatt caaaagtatt tttgtctctt ggtttattcc tgtccggact ggtaaatttt
     1081 ctgataggat atattcccgg gatcatcaca tccgttacgt tattttcaac aatgtattta
     1141 cttaacggct ggatccaagg tatgggatat cctcctggtg ccaaaacact ggtgttctgg
     1201 tatgaacatc gggaaaggat cacttgggcc acactgtgga atttatcgca caatgtcggg
     1261 ggagctctgg cccccgttct gattggtttc agttttggtt ttttcggcga cagtgctctg
     1321 gagcatgccc gggccgcatt tatcttccca ggagtactgt gtatggcaat gagtgtgctg
     1381 atttacttta ttcaggtgga cagacctgta tcagtggggc ttcctcccat tgaagaatgg
     1441 aaaggtaacg tcgtttcaca cccggctaaa ggccgtgaac aaggtccgag actctccatt
     1501 cctgatatca tcagaaaaca tatcatccga aataataaac tcatttactg ctgtatctat
     1561 ggttcgtttg tttatattct gagatacggt attgtttcct gggctcctaa gtttttaagt
     1621 gacagtctgg atgtcggtgg taaggatatg ggaaaactgg catcaatggg ggggttttct
     1681 gtttttgaaa tcggtggtgt ggctggtatg cttcttgccg gatacctgtc tgtcagactg
     1741 ttcagaaact caaaaccatt aacaaatact ttatttctgg cacttaccat tattttatta
     1801 atcgcgtact ggtacgtgcc atcaggcaat gaatatctct ggctaaacta tacaatcctt
     1861 attctgttgg ggctggcagt gtatggtccg gtgatgttta ttggtcttta ttcaatggag
     1921 cttgttccta aagaagctgc cggagccgca tcagggctgt caggcacatt ttcctatatc
     1981 ttcggctcaa ttgttgcgac actgggaat
//
LOCUS       DADLTF010000299         1969 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.304, whole genome shotgun sequence.
ACCESSION   DADLTF010000299 DADLTF010000000
VERSION     DADLTF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.304"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..1178)
                     /locus_tag="JN749_004242"
                     /pseudo
     CDS             complement(1..1178)
                     /locus_tag="JN749_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1388..1831)
                     /locus_tag="JN749_004243"
     CDS             complement(1388..1831)
                     /locus_tag="JN749_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370399.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
BASE COUNT          460 a          515 c          501 g          493 t
ORIGIN      
        1 cacaggtctg tcgccccttc agttgccagt tcgctgtctt tacagacgta atcttttttc
       61 atgcgaccga aggcttccct gacaaaatca tcgacctagg cttcatcaac aggaagggtt
      121 ccgtttttcc gtaactcatg aagcgcacgg ttagccaggt atgcctggcg tgaggatccg
      181 ggataaacgc gggaggcggg cagcacatcg ctgttcagca ttgccggtac cttctgacag
      241 actgcatcag ccccatcgcc attacggtct ttttcatcaa atttattgac cagcacaaac
      301 aggcggccga ctgacacatc agcaatggca ttaagctctt tacgaacctc ctcatcggct
      361 ttcgagttca tctgggtgta gtccatcacc gccagcaccg cagaggcttt ctgcagttga
      421 tcccgcatca tcacttccat ctgcggttgt ccggcttcat tagggccggg ggtatccacc
      481 agtgtgagtg ttccctggca ttcatccatc cccaccagat ggctgaattc cacgtcgatc
      541 accggcagtt tctgcacttc tgcgtattca tcaaaaggaa attcactccc cattgccgca
      601 gcaagacgaa ccagatcgtt taacgatgcc agtccggtaa agattccctc ctccccgtgg
      661 tattcatttt tgagccagcc atcatccgtc agaatatcca gcagttcgcg ggtatccccg
      721 gtttgctgca gggtctgtgc gacctgctgt cctgccgggg tggcgagttt ttcctgcagt
      781 gtgattaata aattgcggac aggctgaata tgttccagat ggagaaccgg ctcagttttt
      841 ccgggaacgt ggcggatgag cgtcggtaca gaggtcatgg ggcggttacg gttaggcaga
      901 atttcctgcc ccacaatggc gttgatggtg gttgatttgc ctgctttcat cgtgccgaca
      961 acagccagca ccatctccct gcgggcggtt ttgatttgtt cgccctccag ctccttaatt
     1021 cttttcagtg ctttctgttt atcaaaaagc tgcccgtttt cgttcttacc gtcagataac
     1081 acatccggct catcaaccat ttgccggagc agattgatgc tcagttgcaa aagccggtcg
     1141 gcttcatcac aaagcagggt gatgttcttt tcgtgcatta tttaaccttc ctgagacgca
     1201 ttttcatgac aaccacggag gaggccattt actacaagcg atcgcttata acgatcaata
     1261 taatagaaat gatcgtcata ttctattaat atgaaaaaca caacgcaagg gggacaaccc
     1321 gggtatggct gcgtttgcat tgtgttttca tagagttaaa aatctgtaac gacaacccgg
     1381 cacatcacta ccgggttaag aagaaaatcc agttccatac cgcgcgggcc acagagacca
     1441 cggtgtcacg aacggcgcgc agggccgtac ggacaacaga ggcaacacag acactctccg
     1501 ccgtgtcaaa tatccggtcc accgcaccgg taaactgttc acgggtctga gtgcggacag
     1561 actccgtgcg cagttcgtcc tgcaggcgtg tcatcagagg gatggcggca tgtccgggaa
     1621 gcactgtcat gagtgcactg accggggtga cctccccctg aaacagtgca ttacagagtg
     1681 aggatttacc ggccccgctt ttacccataa tgccaggcac gggttcgtga cgggggagtt
     1741 tgcgcagatg ttccaggatg tgacgggaaa tcgggtaagg cagggaggag agcggttttt
     1801 caattgcctc aatggcatca gacggattca tacatgtatt cccagtgaaa aaagacaaaa
     1861 ccccgtgcac cggaaaggtg gcgggggatt cacggagata atatctttct gaaattattt
     1921 tgaatgcagg tatatgtggc tgatttgtat cagctaatga gtacaggtc
//
LOCUS       DADLTF010000300         1946 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.305, whole genome shotgun sequence.
ACCESSION   DADLTF010000300 DADLTF010000000
VERSION     DADLTF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.305"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            134..625
                     /locus_tag="JN749_004244"
     CDS             134..625
                     /locus_tag="JN749_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370400.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            709..1698
                     /locus_tag="JN749_004245"
     CDS             709..1698
                     /locus_tag="JN749_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370401.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGAEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          481 a          513 c          482 g          470 t
ORIGIN      
        1 cgcttaaccg ttagtagctc acctactcaa tacctaattt aaacaccttt tgagatgacc
       61 gatgcgggga gacccgtagg ccttactgtg cctgcgatac cgatagtctc aactctcaag
      121 aggagaatta cctatgatgt tcaacaacaa caactggaag ctgtcagtca ccgacatcaa
      181 cctttatgaa aacactgtga gtctggacgg tcagtcctat ccgttgtcgc tcgctatcaa
      241 gaccctcatt ccgggctacc tgtcaggctt accgtccacc agccgcgagg cgatggagtt
      301 actggaagct ctggctgaag ctggcgtgac cattggtaac ttcttcagta atgacctgat
      361 gaccgcctac caacgccgcc agatgaacaa acgagctgaa gctgagcgta tcgctaaaga
      421 gctagcgtct cagaaagagc gtacccgcga aatgttcatg accgaagacg aatggcaaaa
      481 agagctacag cgccgcgaac aggtcaaagc tgaacgccgg acctatggtg aaaacctccg
      541 cagcgctact cattccgctg gtcgctcacg tgccgctatc gtcgctgacc ttgagtcagg
      601 cggtaactgg atggacagct tgtaagcaag cgtccctcat ctgagaacac acggtccctg
      661 actgcctgtt agggacttca agtatttgac tcaaggagat taattataat gaatatgact
      721 aaaaacgctt tccagtttgg cattgagcct gttcatatca ccgacaacga caaccttcag
      781 gttaatgagg gccttccgac taacgccgac cctcaggtct atgccctcca gttagctaag
      841 actgttaagg ctatgctgaa cggtgtactt aaagacgctc aagataacat cccgttccct
      901 gtagaagtcc tgccgacccg taacagctta ccgactccaa tcattgctca cactctggct
      961 gaccgctctg tgctggtccc tgtccgtggt ggtaagcgac ctgaagttgt gaccgctccg
     1021 tctggcgctg aaatcaccgt tgagccgatt gaacaggcga tcctcgtgtc tcaccaaaca
     1081 aagctttggg accagaagtc gaccactggt ttcactcagg ggacgctcca gcaggacgca
     1141 ctgaacatct gcgataacgt gattcgtacc attaactcta agatggttga cgtgctggag
     1201 tcctcaaagc tgctgaagac cgtagaactt ccggccctga ctggtagcct gaccgctaag
     1261 gctgacgcta tcatggatgc cctgtacgag aacacggagt cttctttcgg ttctgaggtt
     1321 tctgactacg gcattatcgc ccatgagtca cacctcaagg ctctctcccg tctggctgct
     1381 aagcagggct tcggtggtga agacgctatc gtcgatatgc tggggactga cgtcgcctat
     1441 tacaacggtg aagaccgtgg cgtgttcatg atggctaagc gttttacggc tctgagcttc
     1501 ggttgcttcc gtcacgatgg cgagagcatt actgtggtcc tttcacgtga tggagactcc
     1561 cagtctcacg accttgagat tctcggcaag gtgtttgttg tagctgaagc tgctacgacc
     1621 atcaagatgg gtactggctc ggctactgct gtcctgcctg tagtcaaacg cctgagcttc
     1681 actaaagaag ctaactaaca ataacgacta ttggtcagac ttccgaactg gatgtctggc
     1741 ctttattttg tactagtaat ctagttcgtg aatacagaaa gtaggagggc caaatggacc
     1801 tacacaaagt ctatctcgtg gtcttcctgc taaagctggg actggtggtc tggctatggg
     1861 ctattagacg atgagttttg actgaaaaat gtgagacctc atctaatata aagagcgagc
     1921 caaaagcccc catagcctga cctgag
//
LOCUS       DADLTF010000301         1931 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.306, whole genome shotgun sequence.
ACCESSION   DADLTF010000301 DADLTF010000000
VERSION     DADLTF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.306"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..212
                     /gene="yfdR"
                     /locus_tag="JN749_004246"
     CDS             <1..212
                     /gene="yfdR"
                     /locus_tag="JN749_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HAY7370402.1"
                     /translation="VMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVI
                     PLAPGHAYGMFMERFNELSELRKCA"
     gene            203..565
                     /locus_tag="JN749_004247"
     CDS             203..565
                     /locus_tag="JN749_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HAY7370403.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDAEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            565..870
                     /locus_tag="JN749_004248"
     CDS             565..870
                     /locus_tag="JN749_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370404.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGNNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            870..1220
                     /locus_tag="JN749_004249"
     CDS             870..1220
                     /locus_tag="JN749_004249"
                     /inference="COORDINATES: protein motif:HMM:NF024139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7370405.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
     gene            1097..>1931
                     /locus_tag="JN749_004250"
     CDS             1097..>1931
                     /locus_tag="JN749_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7370406.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVVTKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CT"
BASE COUNT          612 a          415 c          484 g          420 t
ORIGIN      
        1 cagttatgag tacgcccgtg aaatatgccg atctcatcat gctggcaacc gaacgccgcg
       61 atctcgggct tgatgatggc tctttctggc ctgtactgga aggtatcccg gcaacagaga
      121 tgttcaacgt gattccactg gcaccgggcc atgcctacgg gatgtttatg gaacgcttta
      181 acgagttatc ggagttacgc aaatgcgcat gaatgttttc gaaatggaag ggtttcttcg
      241 tgggagatgt gtaccgcgag atctgaaagt aaatgaaaca gatgctgaat acctggtgcg
      301 taaattcgat gcgcttgaag ctaaatgtgc agcacaggaa aacaaagtaa taccagtgtc
      361 aactgaactg ccaccagcaa atgaaagtgt tttgttattc gatgctaacg gagaaggctg
      421 gctaattggt tggcgttctc tctggtacac ctggggacaa aaagaaaccg gagaatggca
      481 gtggacattt caggtcgggg accttgaaaa cgtcaatatc actcactggg cagtaatgcc
      541 aaaagcaccg gaggctggag cataatgacc acttttaccg acaaagaact gattaaagaa
      601 attaaagagc gtatcagcag ccttgacgtg cgagacgata ttgagcgccg tgcttatgaa
      661 atcgcactcc tatctctgga agtagaacca gatgaacgcg aagcttatga attattcatg
      721 gaaaagcgtt tcggtgactt agtagatcgt cggagagcaa aaaacggcaa taacgaatac
      781 atggcatggg atatgactct cggttggatc gtctggcagc aacgagctgg tatccatttt
      841 tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca ctcgatgagg
      901 cctgtcattt tctcaagata tccagaccga ctgccattaa ctggatacgc acagggcgtc
      961 ttcaggcaac acgcaaagat cccactaaga ataaatctcc ttacctcaca acacgacaag
     1021 cctgcattgc ggctcttcag tctccgctgc atactgtcca ggtgagcgcg ggtgatggca
     1081 taacagagga aagaaaatgt cactcttccg cagaggtgaa atatggtacg ccagtttcac
     1141 attgccgaac ggtaaaagat ttaaacagtc tcttggaaca aaggacaaaa ggcaggcgac
     1201 agaactccat gacaagctaa aggctgaagc atggcgggtc agcaaacttg gtgaaatacc
     1261 tgatataacg ttcgaggaag cgtgtgtcag gtggcttgaa gagaaagcac ataaaaaatc
     1321 actggacgat gacaaaagcc ggatcggatt ctggcttcaa catttcgcag gaatgcaact
     1381 aagagacatt actgaatcaa aaatttattc agcaatgcag aaaatgacga accggcgtca
     1441 tgaggaaaac tggaaactca gggcagaagc atgcagaaaa aaagggaaac ctgttccaga
     1501 atacacgcca aaaccagcgt ccgttgtaac gaaggctacg catctttcat ttataaaggc
     1561 cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa ttattaaagt
     1621 gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac aaaggctgat
     1681 tgatgaatgt ccggagccat taaagtctgt tgttgaattt gcactggcaa caggcttaag
     1741 acgctcgaac atcatcaacc ttgaatggca acaaatagat atgcagcgcc gggtggcatg
     1801 gataaacccg gaagagagta aatcaaaccg cgcaatcggc gttgcgctga atgatactgc
     1861 atgtcgcgtt ttgaaaaaac aaatcggaaa tcatcaccgt tgggtatttg tgtacaagga
     1921 aagctgtacc a
//
LOCUS       DADLTF010000302         1918 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.307, whole genome shotgun sequence.
ACCESSION   DADLTF010000302 DADLTF010000000
VERSION     DADLTF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.307"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(87..1499)
                     /gene="pykF"
                     /locus_tag="JN749_004251"
     CDS             complement(87..1499)
                     /gene="pykF"
                     /locus_tag="JN749_004251"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HAY7370407.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          481 a          445 c          470 g          522 t
ORIGIN      
        1 attttttatt gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat
       61 atacaaatta attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg
      121 ccgctcggta ccagtgcacc agaaaccata actacgacgt cacctttgtg tgccagaccg
      181 ctctgcagag ccagttcttt acccagacgg tagaaatcat cagtagaagt gatctcttta
      241 accagctgcg gcacaacgcc tttgctcagt accaactgat gagccgtttt ttcgttggtg
      301 gtcagtgcca ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg
      361 ccctgggtag caaccacgat cagcggagca tccagttttt cagcagtttc aacggcacca
      421 cggcataccg cttcggtgat gcgcagttta cggttgtcat tgttgaactc gagacggctg
      481 ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg
      541 tatttacctt ttgcggattc accagacagc atcactgcgt cagtaccgtc gaggatggcg
      601 tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc
      661 atctgggtcg cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc
      721 tgggcgaaga taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg
      781 atgccgtcag aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt
      841 ttggagatga tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata
      901 acgtcagaac gcttacgaat aaaggaagca gcaacaaagt ctacgccttt ttcgcaacca
      961 aagatcaggt cctgtttgtc tttttcagcc agtgctggca gagcaatgga aacgccaggc
     1021 aggttcacac ctttgttttc gcccaggtca ccgttgttca gcactttaca gataactttg
     1081 ttaccttcaa tggcggtaac ttccataccg atcagaccat cgtcaaccag tacggtgttg
     1141 ccaacagaca ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg
     1201 ataacagatt tatcagtggt gaaagtaaag gtctgaccag ctttcagaga aacatcgtta
     1261 ccgccttcca gtttcatggt gcggatttcc ggacctttgg tatcaagcag gatagcggcg
     1321 gttttaccag ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca
     1381 tagtcaccat gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct
     1441 aacatctctt cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg
     1501 acagtcttag tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg
     1561 gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct ctaaggatag gtgacatcga
     1621 aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag
     1681 tcgttgagtt ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat
     1741 acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgtt
     1801 agacaataaa ttgttatcaa agatttaacg ttccagggaa aaggttacgc aaaaacttcg
     1861 ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag gataagtgaa agttcatc
//
LOCUS       DADLTF010000303         1896 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.308, whole genome shotgun sequence.
ACCESSION   DADLTF010000303 DADLTF010000000
VERSION     DADLTF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.308"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(106..1560)
                     /gene="ospC2"
                     /locus_tag="JN749_004252"
     CDS             complement(106..1560)
                     /gene="ospC2"
                     /locus_tag="JN749_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HAY7370408.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          605 a          321 c          293 g          677 t
ORIGIN      
        1 tgcaatgtgt tgttgcaggg gaacggtctg caccatcccc tatatcgatc aatataacaa
       61 aaatgacaac gtcagattca gggcacaacc taaatatatt ttaggtcacc tcccaaatcg
      121 tttgcctgat accgctccat tttttaataa aaaatcaatt gtttttgaat ctttgctttt
      181 cattgcattg tcaagcatag tgtccccact atttgctttt tgaaatcttt tgtttacatc
      241 aaccaatcca ttattaataa aatattctag cactttatag ttggcactat cttcactaag
      301 taaatactca acatcataca gctcacaata catattgttg ttcattttct ccatctctgc
      361 gacatcttgt tttgtaaaat taaatttaga tagtaaataa agtgccatat cagattttgc
      421 attgcctaga gaataagcaa gagcttgatg agccattttt ttatcagtca ccttattgtt
      481 aatttcttca taattggatg ctttaatggc atcctccaaa gatatatctc tgagtcttat
      541 ctttttgaag ttatctgtgc ttagcattct tggtatatga tactcaagct gaaacacaaa
      601 gtttataatt ctatccagac taacaggatc aatgttttta ttataacaaa actctcggaa
      661 tctttgatct ttactctttc ttataaaatc aataagatgc aacccaattc caagcctcat
      721 atcttttgta ttaaatattg gcacatcagt ttttccctga ttaccatgca catacctgct
      781 aacttcatcc acaacctctt taaagttatc taagaaaaat gattggtgct catggtaaaa
      841 aacaggtgga atagcattat caaagtggtc ggttaatgtc atatatccat atggagagtc
      901 atgatcaatg atatacgcat tagcaccaaa atccacagta tgatgtgttt tatttagtgg
      961 gagtgtttct tgatgattgg aaatttcaac cccaaaaaaa acaaaatcgt ggttagctat
     1021 ttcttcaata tcctcagaga atgtgttttt atatgtttgt atattctcag acttaatttt
     1081 atcaaaggac ttaatattaa gagtattatt tgacaggtta gcgttacttt gatgtctgaa
     1141 gttaaaatca gaattaataa taatagagag caagtgacgt tcatccgaag aaagaggatg
     1201 agttttttgt attatttcag aaagatagtg tctggacacc ttatcgaggt gctcaagtga
     1261 ccgaaatgat gaccgtgctc cagatggcgc ttcaatagaa agatttaatg atttgaaact
     1321 ggtcccttga gagaacattt tactataact ttgcgctgct atagacttcc ttaaaaagct
     1381 tttcacagta tcagcacagt gatctaaatg tttttcactt atgccagaag aggatgagtt
     1441 tgttacgcat gatatgtttt tttgtaatgc ttttgtatct ttgttcggat ttatttttcc
     1501 atcaacaaga tcaatattgt tctgaacatt aatatgattt actgcttcag gtattttcat
     1561 aaaacatccc ccattatgtg cgtttttatc tgcagttatt tagaataata ttatctaact
     1621 taatattttc ttgtatgtat tataacgaac atattttaat atgttgtacg attttcactc
     1681 tgaaatcccc tgagtttaat cacacttcct ttactgaaag gaattattgc aacagtatta
     1741 tttgtttttg gatatatatc ttgcgccttg ttatgtagtt aatgctcaga atatgataga
     1801 taaaatagag acgcaaaaca gggcatgctc ataacatgat gataacataa aaaattagta
     1861 acaataaatg aaagaatcat gggcgctcaa caaaaa
//
LOCUS       DADLTF010000304         1879 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.309, whole genome shotgun sequence.
ACCESSION   DADLTF010000304 DADLTF010000000
VERSION     DADLTF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.309"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1480
                     /locus_tag="JN749_004253"
     CDS             <1..1480
                     /locus_tag="JN749_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7370409.1"
                     /translation="FAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIVEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            1488..>1879
                     /locus_tag="JN749_004254"
     CDS             1488..>1879
                     /locus_tag="JN749_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370410.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTG"
BASE COUNT          521 a          367 c          458 g          533 t
ORIGIN      
        1 gtttgcgttc attattatcg tgttgctggt ggtatatttc atgaccactc gtgtgttccg
       61 tcggcaattt tctgaaatga ccgagctggt taatacgctg gcgtttttgc ccgactcaac
      121 ggatcagatc gaggctctga aaattcgcga aggcgatgcg aaagagatta tcagcatcaa
      181 aaattcgatc gtggaaatga aagatgccga aattgaacgg tcaaataaat tgctctcact
      241 gatctcttac gatcaggaaa gcggttttat taaaaatatg gcgattattg agtccaacaa
      301 taatcagtat ctggctgtgg ggatcatcaa actgtgtggt ctggaagccg tggaagcggt
      361 gtttggtgtt gatgaacgca ataaaatcgt caggaaattg tgtcagcgaa ttgccgagaa
      421 atatgcgcaa tgctgcgata tcgtgacatt caatgccgat ctctatttac ttctgtgtcg
      481 ggaaaatgta cagacattta cccgtaaaat agcgatggta aacgattttg acagcagctt
      541 tggctaccgc aatctgcgca tccataagtc tgccatttgt gaacctttgc agggggaaaa
      601 cgcctggagt tacgcagaaa aactgaaact ggcgatttcc agtatccgtg accatatgtt
      661 ctccgagttt attttctgtg atgacgcgaa actcaacgaa atagaagaga atatctggat
      721 tgcgcgtaat attcgccatg caatggaaat tggcgaacta ttcctcgtct atcaaccgat
      781 cgttgatatt aacacccgcg ccattctgag cgcggaggcg ttgtgccgtt gggtttctgc
      841 ggagcggggg atcatttcac cgctgaagtt cattaccatt gctgaagata tcgggtttat
      901 caatgagctg ggttatcaga ttattaaaac ggcaatgggt gaattcagac attttagtca
      961 acgtgcgtcg ctgaaggatg atttcttact gcatattaat gtttcgccct ggcagttaaa
     1021 cgaaccgcac tttcatgagc gttttaccac catcatgaaa gaaaatggcc tgaaggcgaa
     1081 cagcctctgt gttgagatca ctgaaaccgt gatcgagcga attaatgaac atttttatct
     1141 caatattgaa caactgcgta aacaaggggt acggatatcg attgatgact ttggcaccgg
     1201 tttgtcaaac ctgaaacgtt tttatgaaat taatccagac agcataaagg tggactcgca
     1261 atttaccggc gatattttcg gtactgcggg aaaaattgtg cgcattattt tcgatctggc
     1321 acgctataac cggatcccgg tgattgcgga aggcgtagag agcgaagacg ttgcgcgcga
     1381 attaattaaa ttaggatgcg ttcaggctca ggggtatctg taccagaaac ccatgccatt
     1441 ctccgcctgg gataaaagtg gaaaattagt aaaagagtag tttacgtatg tccagaatca
     1501 ataagttcgt acttacagtc agtctgctga tttttatcat gatttcagca gttgcctgcg
     1561 ggatctacac tcaaatggta aaggaacggg tgtatagcct gaaacagtcc gttattgata
     1621 ctgcttttgc ggtggcaaat attgctgaat atcggcgtag cgtggcaatt gatcttatca
     1681 acacgctaaa tcccacggag gaacagctgt tggttggttt gcgcacagct tacgccgact
     1741 cggtttcccc ctcttatttg tacgatgtcg gtccttatct gatttccagt gacgaatgta
     1801 ttcaggtaaa ggagttcgag aaaaattatt gtgcagatat tatgcaggtt gtgaagtatc
     1861 gacatgtcaa aaatacagg
//
LOCUS       DADLTF010000305         1877 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.310, whole genome shotgun sequence.
ACCESSION   DADLTF010000305 DADLTF010000000
VERSION     DADLTF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.310"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..557)
                     /locus_tag="JN749_004255"
     CDS             complement(<1..557)
                     /locus_tag="JN749_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7370411.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JN749_004256"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="JN749_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HAY7370412.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1544)
                     /locus_tag="JN749_004257"
                     /pseudo
     CDS             complement(1321..1544)
                     /locus_tag="JN749_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          536 a          319 c          395 g          627 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agcgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccaggctt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tggttaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 cgaaatagaa ttaccgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac tccacaaatg
     1681 aaatatacac cggttggcgt tgatatggca aaacatgtca ttcagattca cttcatcaat
     1741 gagcacatgt cagcgccagt gatataagac ggtaatgggg atgcggacga tttcctggac
     1801 tctcagaaga tgaaacatgt ttactgaaga acaaaaaatt cgcgctattg agctttattt
     1861 caaatatggt aagaagc
//
LOCUS       DADLTF010000306         1869 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.311, whole genome shotgun sequence.
ACCESSION   DADLTF010000306 DADLTF010000000
VERSION     DADLTF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.311"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            27..1869
                     /gene="cyoB"
                     /locus_tag="JN749_004258"
                     /pseudo
     CDS             27..1869
                     /gene="cyoB"
                     /locus_tag="JN749_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          357 a          485 c          504 g          523 t
ORIGIN      
        1 attaaagggg ttgaggaaga ataaagatgt tcggaaaatt atcacttgat gcagtcccgt
       61 tccatgaacc tatcgtcatg gttacgatcg ctggcattat tttgggaggt ctggcgctcg
      121 ttggcctgat cacttacttc ggtaagtgga cctacctgtg gaaagagtgg ctgacctccg
      181 tcgaccataa acgcctcggt atcatgtata tcatcgtggc gattgtgatg ttgctgcgtg
      241 gttttgctga cgccattatg atgcgtagcc agcaggctct tgcctcggcg ggtgaagcgg
      301 gcttcctgcc acctcaccac tacgatcaga tcttcaccgc gcacggcgtg attatgatct
      361 tcttcgtggc gatgcctttc gttatcggtc tgatgaacct ggtggttccg ctgcagatcg
      421 gcgcgcgtga cgttgcgttc ccgttcctca acaacttaag cttctggttt actgttgttg
      481 gcgtgattct ggttaacgtt tctctcggcg tgggcgaatt tgcgcagacc ggctggctgg
      541 cctatccacc gctatcggga atagagtaca gtccgggagt cggtgttgat tactggatat
      601 ggagtctcca gctatccggt ataggtacga cgcttaccgg tatcaacttc ttcgttacca
      661 ttctgaagat gcgcgcaccg ggcatgacca tgttcaagat gccagtattt acctaggcat
      721 cactgtgcac gaacgtactg attattgctt ccttcccaat tctgacggtt accgtcgcgt
      781 tgttgaccct ggatcgctat ctgggcaccc atttctttac caacgatatg ggtggcaaca
      841 tgatgatgta catcaacctg atttgggcct ggggccaccc ggaagtttac atcctgatcc
      901 tgcctgtttt cggtgtgttc tccgaaattg cggcaacctt ctcgcgtaaa cgtctgtttg
      961 gttatacctc gctggtatgg gcaaccgtct gcatcaccgt gctgtcgttc atcgtttggc
     1021 tgcaccactt ctttacgatg ggggcgggcg cgaacgtaaa cgccttcttt ggtatcacca
     1081 caatgattat cgccatcccg accggggtga agatcttcaa ctggctgttc accatgtatc
     1141 agggccgcat cgtgttccat tctgcgatgc tgtggactat cggttttatc gtcaccttct
     1201 cggtgggcgg tatgactggc gtgctgctgg cagtaccggg cgcggacttc gttctgcata
     1261 acagcctgtt cctgattgcg cacttccata acgtgatcat cggcggcgtg gtcttcggct
     1321 gcttcgcagg gatgacctac tggtggccta aagcgttcgg tttcaaactg aacgaaacct
     1381 ggggtaaacg cgcgttctgg ttttggatca tcggcttctt cgttgccttt atgccgctgt
     1441 atgcgctggg cttcatgggc atgacccgtc gtttgagcca gcagattgac ccgcagttcc
     1501 acaccatgct gatgattgca gccagcggtg cagtactgat tgcgctgggt attctctgcc
     1561 tcgttattca gatgtacgtt tctattcgcg accgcgacca gaaccgtgac ctgactggcg
     1621 acccgtgggg tggccgtacg ctggagtggg caacctcttc cccgcctccg ttctataact
     1681 ttgccgtagt gccgcacgtt cacgaacgtg atgcattctg ggaaatgaaa gagaaaggcg
     1741 aagcgtataa aaagcctgac cactatgaag aaattcatat gccgaaaaac agcggtgcag
     1801 gtatcgtcat tgcagctttc tccaccatct tcggtttcgc catgatctgg catatctggt
     1861 ggctggcga
//
LOCUS       DADLTF010000307         1852 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.312, whole genome shotgun sequence.
ACCESSION   DADLTF010000307 DADLTF010000000
VERSION     DADLTF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.312"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..559
                     /locus_tag="JN749_004259"
     CDS             <1..559
                     /locus_tag="JN749_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370413.1"
                     /translation="LGLARSSYFYHRAALKAGDKYATIRKMLTDITEFQLPAGKVWLS
                     PVVDCFDGKVVSWSLSTRPDAELVNTMLDSAVETLNAGERPVIHSDRGGHYRWPGWLE
                     RVNAAGLIRSMSRKGCSPDNAACEGFFGRLKTEMYYGRKWSGITPEKFMQQVDAYIRW
                     YNERRIKLSLGAVSPKMYRQQCGLE"
     gene            998..1835
                     /locus_tag="JN749_004260"
                     /pseudo
     CDS             998..1835
                     /locus_tag="JN749_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          454 a          454 c          565 g          379 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatcacggag ttccagctcc ctgcaggtaa
      121 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      181 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      241 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      301 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      361 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      421 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      481 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      541 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      601 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      661 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      721 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
      781 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
      841 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
      901 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
      961 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1021 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1081 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1141 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1201 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1261 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1321 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1381 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1441 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1501 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1561 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1621 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1681 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1741 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     1801 acctggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DADLTF010000308         1848 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.313, whole genome shotgun sequence.
ACCESSION   DADLTF010000308 DADLTF010000000
VERSION     DADLTF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.313"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..356)
                     /locus_tag="JN749_004261"
     CDS             complement(<1..356)
                     /locus_tag="JN749_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7370414.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGVCGGCP"
     gene            complement(356..1276)
                     /locus_tag="JN749_004262"
     CDS             complement(356..1276)
                     /locus_tag="JN749_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAY7370415.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1414..>1848
                     /locus_tag="JN749_004263"
     CDS             1414..>1848
                     /locus_tag="JN749_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7370416.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGV"
BASE COUNT          460 a          455 c          427 g          506 t
ORIGIN      
        1 gggcaccccc cacacacacc cagcacgcca atgcgttcag catcaacata tggcagagtt
       61 actgcataat cgataacacg gctaatatcc tctacgcgct gtgtcggatc ttctatccaa
      121 cgaggctccc caccagattc tccctggaaa ctggcgtcat aagcgagaac gacatagcct
      181 ttttcagcca atgccttgcc atagatattg ccagacgtct gctccttaca actgccaaaa
      241 ggatggacgc tgataatcgc cggatactgt tttgactcat caaaatttgg tggaaactga
      301 atattggcag caatatgcca ggccatatca ctattgcgga tttgaatatg tttcattaca
      361 gagtttcctc aaagaacgga gctaatactg aaattgcttc agccacatag gctttgccgt
      421 cataaagatc catatggttt gcgccttcaa caatgtgata gcgtttatcc tggcttgatg
      481 cccgatcgta caggtcgtca ctcatccatt tgctccctgc ctggctgccc gccacaatct
      541 gcatcggctg agtcaggtac acttccgcca tatggtaagc atcataggta ataatctggt
      601 taaggctgcg caaagtagcg taacccggtg ctgttggata ctgcgcacga ggtgtgtggt
      661 aatactccca ggcctgacgc agttcttcgt tcggcgcatc ggcctccttc attggagcca
      721 gtggcataat ggcgtattct tcgctgctaa tatcgctggt tcgggcgttt gagccagctt
      781 caatgtgtgg cagagcatca atagatttca cattgttttc ccaaccattg cggaacatcg
      841 aaccaatatt gaccgcacta acggtaccga tggccttgat gcggcgatcc tgaattgcag
      901 cattggctgt atatcctgca ccggcacaaa ttcccatcgc accaattcgg gtattgtcga
      961 cataagaaag cgttgtcagg taatcaatca cggcactgac gtcttcagta cgaatgtatg
     1021 ggttttctaa ctgacgcggc tcgccgccac tttcaccttg ataagatgcg tcataagcaa
     1081 tagtgacaaa tcccttttcc gccagctttt cggcataggt tccggccgtt tgctctttaa
     1141 cgcccccacc tgggtgagat accacaattg ccggatactg acgggtttca tcaaattttg
     1201 gagggaaata gatcactgca gacaaagaga tggttggatt attgctgtta gtgaagctga
     1261 ctttattatt catcattcag ttcctcatgg agttgtcggt tcgttttgac ggttggtgat
     1321 atcactatag atattgatca ttaagttgat tagaccctaa atcatgatta gacctatcat
     1381 ttaaatgatt aatagattgt atggagatac tgaatgaagc gtgaagaaat cgctgatctg
     1441 atggcgtttg tcgtcgttgc agaggagcgt agcttcactc gcgcagcagc ccgcctgagc
     1501 atggtgcagt cagctttaag ccagatagtg cgccgtatag aagaacgatt gggattgcgg
     1561 cttctgacgc gaaccacgcg cagcgttgta cccactgaag cgggcgagca tcttttgtct
     1621 gttcttggcc ctatattgca tgacatagat tctgccctga catccctgag cgatctgcag
     1681 aaccgcccat ccgggacaat acgtattact actgtagaac atgcagcaaa aacgatattg
     1741 ttaccagcca tgcgcacatt cctgaaatcg catcctgaaa ttgatattca gcttaccatt
     1801 gattatggtt tgaccgatgt cgtttctgaa cgttttgatg caggcgtc
//
LOCUS       DADLTF010000309         1834 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.314, whole genome shotgun sequence.
ACCESSION   DADLTF010000309 DADLTF010000000
VERSION     DADLTF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.314"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..222
                     /locus_tag="JN749_004264"
     CDS             <1..222
                     /locus_tag="JN749_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370417.1"
                     /translation="IANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGV
                     GPGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            complement(864..1136)
                     /locus_tag="JN749_004265"
     CDS             complement(864..1136)
                     /locus_tag="JN749_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370418.1"
                     /translation="MSYQPGGLVLPDLSRRGYLSKNASGSNRFGLVNGDGLSKDSRCW
                     CTFSRWRSSMDNAIACSSSLVIVCTWKAIYRSTSHYGKNVLLLQLA"
     gene            1225..1404
                     /locus_tag="JN749_004266"
     CDS             1225..1404
                     /locus_tag="JN749_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370419.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGISAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            1456..1650
                     /locus_tag="JN749_004267"
     CDS             1456..1650
                     /locus_tag="JN749_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370420.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          582 a          422 c          360 g          470 t
ORIGIN      
        1 atagccaatt ttatcgcatt acaaataaat ggttcaaaaa caggatttgc cgcatctatt
       61 acaggtttta ctcaaacaat aagcgctggt acaatcagtt tcctgtcagt aaccgtaagc
      121 gacaacggtg ttggtcccgg gactacttta ggtcttggtt cactaataac ttcattactg
      181 tcaatagccg taatcgccat tgcaggaaat aataaaaagt aagtgtactc tgactgcgta
      241 tgtatctact ttgtgtaatg caggtgtcta ttgaaaataa ttcttttcgt gggcatctgt
      301 tgtataaaat aaaaaattat cgtaagtaca tctatctgcg atgtcttgtg aaaattatcc
      361 tgtctggcaa caatcgcgtc catctgtatt aatggacgcg aacgatgaac tctcaaacaa
      421 cactagatat caccggcttt cgcacttatt tgccagaatc attacgtctc agattcgacg
      481 agcaattatc tgtccgggct atcgcacagc agttaagact cagttattcc cccattcaca
      541 atctctttca acgaattaac gcctcaggta ttgtctgacc atgacccgag tccctttcat
      601 tcgcccaacc gataccatac tttatgccaa cagaaagaat gaatgaaccg cgcctaaaat
      661 cagcaaggac acatggcaca aagtgcgaca tgtctcacat ttaaccccag tgggggcaac
      721 aagtataaaa ccgttttttg attaagtggt cgaatatgaa aaggggaagg tttatgtgaa
      781 aaatacctgt gttttctttg aaaacacaca acagtatcag gttatttatg tttatacagt
      841 gcgttccaaa ctgacctgaa taattacgca agttgcaaca ataaaacatt tttgccgtag
      901 tgtgaggtac tccggtaaat tgctttccag gtgcagacaa tgactaacga agaactacag
      961 gcaatcgcat tatccatgct tgagcgccag cgcgaaaaag tacaccagca acgagagtct
     1021 ttgcttaacc catctccatt caccaggcca aaacgattgg agcctgaagc attcttggac
     1081 aaatacccac ggcggcttaa atcaggcaaa accagaccac caggctgata gctcacagtt
     1141 tgttctccga ttcaataaaa cggataagaa ccatcagcgc ggtagcgtaa ggcactacag
     1201 cgcattgctt tacgagtaat aacaatggat ttatcaccag aagacgcaaa gtttttacga
     1261 gaactgcaac accgtatgct cagagaagcc attatcgaac agcaaacggg cataagcgcc
     1321 atagacccat tgcgatacaa acgggcctca tctcctttag ttgacgacaa ttccaatgcg
     1381 gataaaacac gtccaccaca ctgattacat ttttcattag cacagtggtg tgatgcagca
     1441 tacgaggaaa accggatgaa tgagaacaaa ataaaaaggc tggagcaact gttacaggca
     1501 cgccaacggg agtttgccac taaatgggga cgtgccgcgc tgccacatga acgcctaacc
     1561 tattttccac ttgaagacct cattaaaaat aatggcaaaa aaatcaggtc tgataagaaa
     1621 cacttcttag aatgcaatga agacaaataa caacaatcta catattgcca ccaaccacag
     1681 atgtggcatc gttatcagcg cacttgattt tctttatatc cactgacgag cttgccgggg
     1741 cacctttgtt tgccggtcat tctggtatca tgcagatatt tacggcgtac aagcccacta
     1801 gcatgacatt gtaataaggc ctgagcctaa cgct
//
LOCUS       DADLTF010000310         1782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.316, whole genome shotgun sequence.
ACCESSION   DADLTF010000310 DADLTF010000000
VERSION     DADLTF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.316"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..452
                     /locus_tag="JN749_004268"
     CDS             <1..452
                     /locus_tag="JN749_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370421.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            455..988
                     /locus_tag="JN749_004269"
     CDS             455..988
                     /locus_tag="JN749_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7370422.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="JN749_004270"
     CDS             complement(1017..1544)
                     /locus_tag="JN749_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7370423.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="JN749_004271"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="JN749_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435977.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          498 a          392 c          399 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggacgtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcagag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DADLTF010000311         1773 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.317, whole genome shotgun sequence.
ACCESSION   DADLTF010000311 DADLTF010000000
VERSION     DADLTF010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.317"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..284
                     /locus_tag="JN749_004272"
     CDS             <1..284
                     /locus_tag="JN749_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370424.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="JN749_004273"
                     /pseudo
     CDS             286..890
                     /locus_tag="JN749_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1773
                     /locus_tag="JN749_004274"
     CDS             877..>1773
                     /locus_tag="JN749_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370425.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          425 a          403 c          496 g          449 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggcaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 tcagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttc
//
LOCUS       DADLTF010000312         1758 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.318, whole genome shotgun sequence.
ACCESSION   DADLTF010000312 DADLTF010000000
VERSION     DADLTF010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.318"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..262)
                     /locus_tag="JN749_004275"
                     /pseudo
     CDS             complement(1..262)
                     /locus_tag="JN749_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(377..1015)
                     /locus_tag="JN749_004276"
     CDS             complement(377..1015)
                     /locus_tag="JN749_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370426.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1174..1644)
                     /locus_tag="JN749_004277"
                     /pseudo
     CDS             complement(1174..1644)
                     /locus_tag="JN749_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          499 a          434 c          318 g          507 t
ORIGIN      
        1 gcaagcgctg taactctttt atttgtttta tagcggcagc cagttctgag gcaggaacga
       61 catcctctcc ggcagcaaca gcagtgagac ttccttccag atattatttt ttccacttaa
      121 gcaacaggct gggctgaata ccatgcaaac gcgccacatg ggacaccgtc atgccaggct
      181 ccattgtctg ctgaataatg gcaatttttt cctgaggagt tttacgttta cgaagctcca
      241 gccccagcaa agttccagtc atctcaaaat tagaactagt gttagacata tatccaagcc
      301 tatctcttat tttaagatac atccactgtc tggcgtttca aggggccggc tcaaatctat
      361 gtacaggctc tcagcatcag caaagcctct tacttttcca actactactg ttagcatagt
      421 gatcaaattc ctgatcagat acatgaatta cctgaaccga actattttca tctacaaatg
      481 catctggcga tttttctaca ggatcataaa gattaaatcc ctttcctgtt tgaacatcca
      541 aaaacataat tttttcacca tcgttaaatc cagctatcca atgagtggcc tgttcaatag
      601 aaatcattac tacacttcct tttgaagtat tttgtaaaat attttctgca atacgctcac
      661 gaaatttact ataagtttca ttaggatgtg acactatgcc ttcactatca atttgctcta
      721 aatgcctatg aaaaaatccg gtcatatact ctgatatttc attaggtacg ggagcctctc
      781 ttactccata ccctaacata gccatacaag catgaagcgc acaccctgaa cagttaccat
      841 ccccaccaga cggatttatc attttctgta aagtcgctgc caattcagaa tctttatcca
      901 tatcaccaag acttttagag tttaaagatt ccagcaattt tttaacattt acatttccct
      961 cctctgtatg tgcctcatat ccagcagttc cctgtaacga caccccatta atcatattgt
     1021 atcctgacag tatatatact cactatattt tcttcataac aaatatccct ctgatatgac
     1081 aacaaaaaac ggagccggat tcctgttttt gtgaactgca tctattcgtg tctaaaaagt
     1141 gatccactta ccaccaacgg cctaatatat acagaacgga gtaaacaggg ctaacagttc
     1201 ccgacaggtc tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt
     1261 gttgtacgca taccagagcc agtgttgttg cagccttgct gccaacgaag cgccactgct
     1321 catcaagttc acagataagg gcaacatcag cgtgagcgac tggcgcagat gttatcctcc
     1381 ggggcgggag ctttttaatg tccgtatgac ggtattgatg ccgattttca gtgtcctggc
     1441 ggtatcacgg ccccctgcgc cgttatgtgc catttcaacg atctgctcct taacgccggg
     1501 ttttctggct tcataagagt aagtgagctg gaaaacacgt ttacatgagc gacaacggaa
     1561 acgctcatgc cgggaacaac tacggccatg tcgataaact tcatcggaat gacagcgcgg
     1621 acaatgaaca gtaacggtag ccatggaaaa ccttaaatac cagtagatta ccagaaactt
     1681 ctgtcgattg aagacatcac ccgaagtttg aattcattcg aatacttttt acgaggttct
     1741 tttcgccagt cctgtaat
//
LOCUS       DADLTF010000313         1752 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.319, whole genome shotgun sequence.
ACCESSION   DADLTF010000313 DADLTF010000000
VERSION     DADLTF010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.319"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..361)
                     /locus_tag="JN749_004278"
     CDS             complement(<1..361)
                     /locus_tag="JN749_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HAY7370427.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEI"
     gene            complement(426..1376)
                     /gene="virK"
                     /locus_tag="JN749_004279"
     CDS             complement(426..1376)
                     /gene="virK"
                     /locus_tag="JN749_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HAY7370428.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            complement(1381..>1752)
                     /gene="capU"
                     /locus_tag="JN749_004280"
     CDS             complement(1381..>1752)
                     /gene="capU"
                     /locus_tag="JN749_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HAY7370429.1"
                     /translation="MGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAEASA
                     FSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLAKQD
                     IEERFDINKTALKILTLAKQK"
BASE COUNT          532 a          391 c          333 g          496 t
ORIGIN      
        1 gaatttcgag gcctttccag aacactcgtt tctggaactt ttccggacca cgaattgcca
       61 gttctgccat catcactatg gattgcaatg ctgtgacaaa cattttgtcg acaattattt
      121 cccgttctgt atcactcttt tctgggaaac aaagcgataa attaatcgtc gccctgcgac
      181 gagctttttt gctcaatctg cccgcccaac gccctatttt tgccaagaca ggatctctga
      241 atgaaggggg aaacattgaa atacctgcaa tcattcccaa aaggaaccat gtagaccagt
      301 aaacagggga aaggtaattt ttcttaaatt caggaataaa ctcagattta tattttttca
      361 ttatttagtg cgaatgcctc taccaaaatt atcattactg ttttattcag agaatacatc
      421 aaaccttaat ttaaattctg atgttctgag tgtacaggat atgcatccaa agagcggata
      481 gtagtttcat ggatataatc cagcaaagcg tatcgttttc ggtactcaga gcgtttttta
      541 ctggctatat cgcttagcgg ctttcgaatc acctgggttg gtaatctgta ccagtcacca
      601 caggtttttc ctgctacaga ctcccagaaa tcactataga ccgcaacaaa ggttttgcgt
      661 ttctgatacc agtatctcaa ttgccggaaa acatgactgt gctcactgac tgccagacac
      721 tcagagatat tgcaagcttt catcaacgca caaaatgctt caaaaataat ccgttttggg
      781 aaaatcccat aacaggcccg ggttgcgcac cgaataacat caggtccggt atttttaggg
      841 gctccctgta atccgccaat aaaagcaatg tgtccatttt gagtcagaat gactgaaaat
      901 gttaaccggg caataactgt tttattgtaa aacaatgaca acatcaactc tccttcccgg
      961 tcaaaaccac tgggagaaca ctggatatca aaattctctc catctttccc gatgaattgg
     1021 actaatgata ccgatttttg tggaaacaaa aatttagaat atttttctgg caaattttgc
     1081 acaaagcgat aatgctcaag aatatattgt ccacgggtcc aggcgcgccc ccctttatgc
     1141 aaataagggc gatgaattct tgctggcaac gtggggtgta ttgccagaat atcctcaatg
     1201 cacggcaagg aacgcagttc ataataatat cgaatactga caaccggatt tatagaacat
     1261 cgcaaaatgt acatgaatcg aaattttctg tgttcccact tccccggtag tgaatctgag
     1321 gaaaaaataa tccttttaag gaaaccgata aatgataagt tacttataga aaacatattg
     1381 ttacttttgc ttcgctaatg tgagtatttt taacgcagtt ttattaatat caaaccgctc
     1441 ttctatatcc tgttttgcca ggcgagccat ctgataaaaa cgcccagggt cattaaaaaa
     1501 atcattcagg gcgcacatcc atgcgtgctt attacttgct ggtaacaatg tcccggtctg
     1561 gttgttctga ataacctcag ggattccacc aatctgagtg gccactacag gcacagaaaa
     1621 tgccgatgct tctgccagca ccataccaaa agattcgttt tctgaaggca gaaccaccag
     1681 actggcaacc cgatatacgg gggcggcagg aaaaacattg tcagcaataa aaacatcatc
     1741 atgcatgccc at
//
LOCUS       DADLTF010000314         1740 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.320, whole genome shotgun sequence.
ACCESSION   DADLTF010000314 DADLTF010000000
VERSION     DADLTF010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.320"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..366
                     /locus_tag="JN749_004281"
     CDS             <1..366
                     /locus_tag="JN749_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7370430.1"
                     /translation="VYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVV
                     CVQQPMFTEMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLL
                     AGCLISAMTALLMKDNQRS"
     gene            468..>1740
                     /gene="amn"
                     /locus_tag="JN749_004282"
     CDS             468..>1740
                     /gene="amn"
                     /locus_tag="JN749_004282"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HAY7370431.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVSYGTLL"
BASE COUNT          411 a          436 c          410 g          483 t
ORIGIN      
        1 gtttatatca caggcgcgtt gatcggaacg ttgagcgcat ttcctttctt tatggcgctt
       61 gaagcacaat ctattttctg gatagttttc ttctccataa tgctggcaaa cattgcgcat
      121 gacatggtgg tgtgtgtgca acaaccgatg tttaccgaaa tgtttggtgc cagttatcgc
      181 tacagtggtg ctggagtcgg ttatcaggtt gccagtgtgg ttggcggtgg atttacacct
      241 tttattgccg ctgcactcat cacttacttt gccgggaact ggcatagcat cgccatttat
      301 ttgctggctg gatgcctgat ttctgcaatg accgctttat tgatgaaaga caatcaacgc
      361 tcgtgatagc ctgccgaaga tcatccgatc ttcgccttat acttttgttt cacattcctg
      421 tgacatacta tcggatgtgc ggtaattgta tggaacagga gacacacatg aataataagg
      481 gctccggtct gaccccagct caggcactgg ataaactcga cgcgctgtat gagcaatctg
      541 tagttgcttt acgcaacgcc attggcaaat atattacaat tggcgaatta cctgatgaaa
      601 acgcccgtaa acaaggtctt tttgtctatc catcactgac cgtaacctgg gacggtagca
      661 caacaaatcc ccccaaaacg cgcgcatttg gtcgttttac tcacgcaggc agctacacca
      721 ccacgattac tcgccctact ctctttcgtt cgtatctgaa tgagcaacta acgttgcttt
      781 atcaggatta tggtgcgcat atctctgtgc aaccctcgca acatgaaatc ccttatcctt
      841 atgtcatcga tggctctgaa ttgacacttg atcgctcaat gagcgctgat ttaactcgct
      901 acttcccgac aacagaactg gcgcaaatcg gcgatgaaac tgcagacggc atttatcatc
      961 caacggaatt ctccccgcta tcgcattttg atgcgcgccg cgtcgatttt tccctcgcac
     1021 ggttgcgcca ttataccggt acgccagtag aacattttca accgttcgtc ttgtttacca
     1081 actacacacg ttatgtggat gaattcgttc gttggggatg cagccagatc ctcgatcctg
     1141 atagtcccta cattgccctt tcttgtgctg gcgggaactg gatcactgcc gaaaccgaag
     1201 cgccagaaga agccatttcc gaccttgcat ggaaaaaaca ccagatgcca gcatggcatt
     1261 taattaccgc tgatggtcag ggtattactc tggtgaatat tggcgtggga ccgtccaatg
     1321 ctaaaaccat ctgcgatcat ctggcagtgc tacgcccgga tgtctggttg atgattggtc
     1381 actgtggcgg attacgtgaa agccaggcca ttggagatta tgtccttgca cacgcttatt
     1441 tacgcgatga ccacgttctt gatgcggttc tgccgcccga tatacctatt ccgagcattg
     1501 ctgaagtgca acgtgcgctt tatgacgcta ccaagttagt cagcggcagg cctggtgagg
     1561 aagtcaaaca gcggctgcgt acaggtactg tggtaaccac agatgacagg aactgggagt
     1621 tacgttactc agcttctgca cttcgtttta acttaagccg ggccgtagca attgatatgg
     1681 aaagtgcaac cattgccgcg caaggatatc gtttccgcgt gtcatacggg acactactgt
//
LOCUS       DADLTF010000315         1729 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.321, whole genome shotgun sequence.
ACCESSION   DADLTF010000315 DADLTF010000000
VERSION     DADLTF010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.321"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            457..1530
                     /locus_tag="JN749_004283"
     CDS             457..1530
                     /locus_tag="JN749_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR"
                     /protein_id="HAY7370432.1"
                     /translation="MHTRTKLPAPLAADVAALIKNGMDFLDKAREEFEAKQYKHSVVS
                     FWIAVEILLKVPLASEHWTLVCSGKKVSRKSYLAGDFQSVSFDDVCTRLRDILEKPLP
                     KETEAVFNTIRNHRNRVVHFFHTAFSDSEVETILAEQARAWFALNRLMREDWQQHFAS
                     PHNWALALGETQLLRGNEFYAEARLKHIQPELEQLATEGAEFHPCTICHKPAAIMEIL
                     AVGKNGPTVYEQTCRVCFHSERHVKFTCPECDTDQVLPVEEEDDDTFICRTCNAELSR
                     YNLLDEENFRHVDEMMYPDGLANCAHCEGHETVCVFGENFLCTRCLEIHTGYDTCEIC
                     GTPCEAMGETMRYTGCPHCADED"
BASE COUNT          448 a          415 c          428 g          438 t
ORIGIN      
        1 cagaaagtag tcgtaagaac ggtgatcact gtccgctttg tgccaggagc ggacgttaag
       61 ggtttccaag ggagctatct aatatggcat ccacggtgaa cgcattgcca gccgggaaat
      121 gatgcgaata actgtgttta attttatcga gtgagattac aatcggtggc gaaatcactg
      181 tgctgtggcg gactcagctc ggaatttatt gaaactataa cctcgctagg gtcttgctca
      241 cattgggtag gatcagtctt gtaatgcacc tgttgtatgg ccttgttcat ccgaagatcc
      301 tgcaatggct gatggtgcat gagaagtttg agtgcaatga ggtcaacacc aaagtctttg
      361 ccctcgatgt ggtggccagc cagcaactaa gatttatcct ctatacccac taatctctgc
      421 agcagctgcc ggccccccgt gataacggag atcaggatgc atacacgaac aaaattacct
      481 gcccctctcg cagctgatgt ggcggctctc attaagaatg gcatggattt tctggataag
      541 gcacgtgaag agttcgaggc caaacagtac aaacactcgg tagttagttt ctggatagct
      601 gtagaaattt tgctcaaggt accccttgcc agcgaacact ggacgctggt ctgcagcggt
      661 aaaaaagtat cgcgtaaaag ctaccttgcc ggtgattttc agtcggtgtc ctttgatgat
      721 gtctgtaccc ggttgaggga tatccttgaa aagcccctgc ctaaagaaac cgaagctgtg
      781 tttaatacca ttcgcaatca ccgaaaccgt gtagttcatt tctttcatac agcttttagt
      841 gattctgagg tggagaccat tctggctgaa caggctcggg catggtttgc actgaaccga
      901 ctaatgcgtg aagactggca acagcatttt gcttcaccac acaactgggc actggcatta
      961 ggtgaaacac aactcctgcg aggcaacgag ttttatgccg aagcccgtct gaaacatatc
     1021 cagcccgagc tggaacaact tgcgactgag ggcgctgaat ttcatccctg caccatttgc
     1081 cacaagccag cagccatcat ggaaattctt gccgtcggga agaacggtcc taccgtttat
     1141 gagcaaactt gtcgcgtctg ttttcactca gagcgccatg taaagtttac ctgtcctgag
     1201 tgtgatacag accaggttct gccggtggaa gaagaagatg acgatacctt catctgccgc
     1261 acctgtaacg cagaactctc aaggtataac ttgcttgatg aggagaattt tcgtcatgtg
     1321 gacgaaatga tgtatcccga tggcctagca aattgcgctc actgtgaggg ccatgaaacg
     1381 gtctgtgtgt ttggcgagaa cttcctgtgc acacgatgtc ttgagatcca tacaggctat
     1441 gatacttgtg aaatttgtgg gactccctgt gaagcaatgg gcgagacaat gaggtatacg
     1501 ggctgcccac attgtgcgga tgaagactga atcgccacaa attcagtagc cttcttttag
     1561 ccttcaacgt ctgctcctcg ctcttagcgg acattagcat aggctattta ccataacgcc
     1621 tcattacgcg taccgctcag acggcctgag cgcgtttctg acgtatcccc gttaaaaaca
     1681 gtaacaaacc acccgaaaat gcacgccaga aacgctacgt aagaatcta
//
LOCUS       DADLTF010000316         1709 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.322, whole genome shotgun sequence.
ACCESSION   DADLTF010000316 DADLTF010000000
VERSION     DADLTF010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.322"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>1709
                     /locus_tag="JN749_004284"
     CDS             <1..>1709
                     /locus_tag="JN749_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HAY7370433.1"
                     /translation="LEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVF
                     VSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGTLQLQHI
                     SPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFR
                     DPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLA
                     YRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQL
                     SLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGYVALNLPAADKPSRYLLTVSASD
                     GAAYRVTTTKELLIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRL
                     EDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTV
                     DIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDT
                     LYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGEL
                     VNV"
BASE COUNT          398 a          446 c          468 g          397 t
ORIGIN      
        1 ctggaaggcg catccagcaa tttcatctcg ccgcagccgg gtaatattta tattcctctc
       61 ggccaacaag agccggggct gtacctcgtc gaggcgatgg taggtgggta tcgggcgacg
      121 acggtggtgt ttgtttccga taccgtggcg cttagcaaag tgtcaggtaa cgagcttctg
      181 gtatggaccg cgggtaaaaa acagggtgaa gcgaagcccg gctcagagat cttgtggact
      241 gacggtcttg gcgtgatgac ccgcggggtg accgatgaca gcggtacttt gcagttacaa
      301 catatatcgc cagaacgttc atacattctg ggtaaggatg ctgaaggcgg cgtttttgtc
      361 tccgagaact tcttctacga aagcgaaatc tacaacaccc gcttgtatat ttttaccgat
      421 cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag tgatgggccg cgagttccgc
      481 gatccgttgc attcatcccc catcgtcagc gccccggcga agctttcggt gctggacgct
      541 aacggcagtc tgttgcaaac cgtcgatgtc acgctggatg cgcgcaatgg cgggcaggga
      601 agtttccgcc tgccagaaaa tgccgttgcc ggaggttatg agttacgtct tgcttaccgc
      661 aatcaggtct atagcagcag ttttcgcgtg gcaaactaca tcaagccaca tttcgagatt
      721 ggtttagctc tcgacaaaaa agagttcaaa actggcgaag cggtcagcgg caaactgcaa
      781 ctgctctacc cggatggcga gccggtaaaa aatgcccgcg tgcagttaag tttgcgcgct
      841 cagcaattat caatggtcgg taacgatttg cgttatgccg gacgtttccc cgtgtcgctg
      901 gaaggcagcg aaacggtgtc cgacgccagc ggttatgtgg cgttaaatct ccccgccgcc
      961 gataaaccga gccgctattt gttaaccgtc tccgccagtg acggcgcggc gtatcgcgtc
     1021 accaccacca aagagctcct cattgaacgc ggcctggcgc attactcttt aagtaccgcc
     1081 gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt atgccgcgct ggaatcttca
     1141 aaacaggttc ctgttacgta tgaatggttg cgtctcgaag accgcacgag ccatagcgga
     1201 gagctaccgt caggcggcaa atcctttacc gtcaatttcg ctaaacctgg caactacaat
     1261 ctgacattac gcgataaaga cggcttaatt ctcgctgggt taagtcatgc cgtcagcggt
     1321 aagggcagca cggcgcatac tggtacggta gatatcgtgg cggataaaac gctgtaccag
     1381 ccaggcgaaa ccgcgaagat gctgattact ttcccggagc caattgatga agcgttattg
     1441 acgctggaac gcgatcgcgt ggaacagcag tcgctgcttt cgcatccggc aaactggcta
     1501 acgctacaac gtttaaacga taccctgtat gaagcccggg ttccagtgag caattccttt
     1561 gcgcctaaca tcactttttc ggtgctgtat acccgtaatg gtcagtacag ttttcagaac
     1621 gccgggatca aagttgccgt tcctcagctt gatatccggg tgaaaacgga caaaacccat
     1681 taccagcctg gtgaactggt caatgtcga
//
LOCUS       DADLTF010000317         1698 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.323, whole genome shotgun sequence.
ACCESSION   DADLTF010000317 DADLTF010000000
VERSION     DADLTF010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.323"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(603..939)
                     /locus_tag="JN749_004285"
                     /pseudo
     CDS             complement(603..939)
                     /locus_tag="JN749_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          551 a          338 c          329 g          480 t
ORIGIN      
        1 aatgtgtgtc atacactcag tggcgtaatc gttgacaata tggtgcatgt aatcgtaagc
       61 gtccagcgaa atccatctgg tcataatctg aagcatccga caaagtggtg tccaccaaat
      121 aagtgatctg cgcccaatat cctgagccgg aactttgaca aaggccaacg aaaaatggga
      181 gcggatgtta cagagttctc ggtacagggg aaaagtggta cctgtcgcca gttctccgat
      241 ctgtttaacc gggaaattat ctcctacagc ttgtcagaaa ggtcggtgat ggagcatcgt
      301 taatacgatg cattcacagt gcccggccag aggatgcccc gtcgctgcat atggatcagg
      361 gttggcaata tcgaatcgca ggctataggc aaagttaaag ccccatggag tagcacaaaa
      421 tatgccgcac aaaggaaacg gtctgaataa cgcagtgatg aagaacttct tcagcacact
      481 gctaaaacgc atagtgatcg agcgctgatt ctggcgaaca actgaactaa tacatcagaa
      541 tctgcattat gttaaataaa tatgaaaaga tggtttaaat accccgttac ttttgactta
      601 cactatacgg tatcgcatcg tttaatattc gcaccggcca gatttttatt tctattagtt
      661 gtcacaatac tgaatgcgta cgaccacagt attctggctc ctgtgtggtt atgctttaat
      721 tctgcgttcc gggcagataa gcagttgctt gcaggaatcc ttcttgtgtt aatgtcagtt
      781 ccccttttta ccagtgctga tttccacatt ccgtccaaca gagcttatag cctttccctg
      841 gattatagca ttgtccggct gaagttcttt ttgaataata atagaagcac tgctggcaga
      901 tccagtccgt ttttcataac ccactgtact gataaccata atctaatcag tagaaattga
      961 gtcgaaaata agcactactc catacaggat aattagaggt cagtttgatt attcacaatt
     1021 catcatcagc attttctatt tctgacgaaa tcaacatgaa aataaccata tatgataatt
     1081 attataataa cggctttaat tggaatacat atattacaac gtattatata taattggtat
     1141 tctgggaact atattctcaa aatacagtag aaacgaggta tgtttctggt ggaaaggaca
     1201 gtgggattaa aaagtaagaa ttgataaaaa aaacgccagc aacacatagt gccggcggag
     1261 ggaatacccc atggagaaaa tgtgatgcct agagcatcat gaatatataa ttaaaaatag
     1321 ttagcgttgt caatactttc acaaaaataa ttttcgtagt atagaaagat atttttatgc
     1381 atgacctacc tgaatttgct ccgggtagag gttataaata aaaattgaat cacgacaaac
     1441 acaatattca tagtatggcg atgcctacgc cagcaagaat agcgccaata atattgggaa
     1501 tataatagac accagacgca caggcatctc actccttaac aaacaataat caggatattt
     1561 acttttaccc agctaactgt ttacacccaa agtgcacaca attaaccatt caataacaaa
     1621 tggcaatatc catagccata cgactttacc ttgtaatgtt cggtatttct ttataattat
     1681 tctgggaaat ctatcatt
//
LOCUS       DADLTF010000318         1678 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.324, whole genome shotgun sequence.
ACCESSION   DADLTF010000318 DADLTF010000000
VERSION     DADLTF010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.324"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(71..538)
                     /locus_tag="JN749_004286"
     CDS             complement(71..538)
                     /locus_tag="JN749_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAY7370434.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(604..843)
                     /locus_tag="JN749_004287"
     CDS             complement(604..843)
                     /locus_tag="JN749_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HAY7370435.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGEIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(978..1583)
                     /locus_tag="JN749_004288"
     CDS             complement(978..1583)
                     /locus_tag="JN749_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAY7370436.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          541 a          370 c          292 g          475 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaagcat ccgatgattc atttatgctg
       61 tcctaaaaaa ttaacaaaac aatgggaaaa caggatatgt aagcagaaaa gccaatgaaa
      121 ttggtacagc ataaggcaca ccgttttcac gaattgattt tcgaaaaaaa agaagacctg
      181 ccagaacaac cagagcgccg caaaaggtca ttgcgacgag aaaattagcc agttcatgtt
      241 catcaatagc taacgaaaga acagttaaca gcttgacatc accgccgcca attatccctg
      301 cggaaaaaag aaagaagccc acgagcaaaa cgctcccggc tgcaaccaga ttgggttttt
      361 gatgctgtaa aatgagtgcc aaaaaaataa gcacggttaa caccagtaaa taacgatggc
      421 taataattct gagtaatata tctccccatg aaataaaaat aagaaagagc gcaacaaata
      481 aaagatcgac tcttagtaac catattgctg ccattgaaag actccatgta ccatacaagg
      541 ttgctgccag acatattcct ttacgtctga cagcctctag agagtttttt tattttttat
      601 aagttacgga ttactcgttc cggaaatcgt tacctgttga attgctgcag cgatagcgtc
      661 gaatgcatct ttaatttctc ccaggaagcc tgagttctga tcgccaaaga tgaaagcaag
      721 cagagttgcc atagcaacac caatcaatgc atattcgatt gcagtaacac cacgttcatc
      781 cttcgcgaaa cgcgcaaagc actgtttagt tttaccatag tacttagtaa taaaagtatt
      841 catgttttcc tcgtatatta ttatttaaat tcaaaccacc agcttcatct gcgtgtcacc
      901 ggtatcccat atttttggga tgtggctata attatccatt cccgctattt agtcttcaaa
      961 aaaaattaca tttaatatca tttatcttga ttaagaaata gtaagactaa aaaaacaagc
     1021 gtcgtcagcc agcacactgc taacacggta agaacgctaa taaaaacact ccactgcttc
     1081 gttctatacc attcaatatt tttgtcagta tgttttattg ggagcagctc ttcagtagag
     1141 aattcctggc tgttatcatt ctcaggaatg tttgcatcag tgtttacttc cctggcctcg
     1201 ctatcgttgg tatagctact actgtcttca tcactgtcac gctcattctc acagacatgt
     1261 ttctcgacct ttcccagata tctgacgcct tttcgcggaa tggttttaat tagagaactc
     1321 gacaaacctg cgacgtctaa tgcctttcta agcatgtaaa tctgcccata ataactgctg
     1381 tcgcttaccg aacctttctg ctcccgccaa acctcattga taatatgttg cttatcggat
     1441 tctccctgta gtaaaaggag taaaaatcga tagccgtttt cagacagatt gattacattg
     1501 cctgtactgg tggtgatacg tcgagacgct gggtaaaact taactccctg ccctaaagaa
     1561 aaataaaaac ggtcatcgct cataagtacc gaaccctgct ttcttcttca catgactact
     1621 cattacaact ttgtatgaag taagatgcaa aatcatattg tttacattaa gttaagca
//
LOCUS       DADLTF010000319         1668 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.325, whole genome shotgun sequence.
ACCESSION   DADLTF010000319 DADLTF010000000
VERSION     DADLTF010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.325"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..132
                     /locus_tag="JN749_004289"
     CDS             <1..132
                     /locus_tag="JN749_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HAY7370437.1"
                     /translation="ASANNIKKRKLYIRSLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            132..302
                     /locus_tag="JN749_004290"
     CDS             132..302
                     /locus_tag="JN749_004290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370438.1"
                     /translation="MTKKIKCAYHLYNKEIEESKIITRPLHFMRGVIPTTEMKKYCSE
                     ICAEKDQMAHEL"
     gene            377..652
                     /locus_tag="JN749_004291"
     CDS             377..652
                     /locus_tag="JN749_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370439.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            752..>1668
                     /locus_tag="JN749_004292"
     CDS             752..>1668
                     /locus_tag="JN749_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7370440.1"
                     /translation="MNTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASS
                     PSVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDEL
                     QALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTA
                     FFEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLT
                     RDVATGVLARSMDVDIYNLHPAHAKRIEE"
BASE COUNT          529 a          374 c          375 g          390 t
ORIGIN      
        1 gcctcggcaa acaatattaa aaagcgaaaa ctttatattc gtagcctgac tacaaaaaca
       61 tgcatttctg actgcatgat taaagtcttc ctcggtcgtg atggtttacc tgtaaaggcg
      121 gagtcatggt aatgactaag aaaataaaat gtgcttatca cctttacaat aaagaaattg
      181 aagaaagcaa aatcattaca agaccacttc atttcatgcg tggagttata ccaacgacgg
      241 aaatgaaaaa atattgtagt gaaatctgtg ccgaaaaaga ccagatggca cacgaacttt
      301 aattaactga ctatccgaaa ctgaatttat gccagcaatg gcagggattc gctcaacctt
      361 aattaaggag aaaaacatga ttaccagtta tgaagccact gttgtaacta ctgatgacat
      421 tgttcacgag gttaatctgg aaggaaagcg tattggctac gtgattaaga cagaaaataa
      481 agaaacccca ttcactgtgg ttgatatcga cggtccatca ggcaacgtta aaacacttaa
      541 cgatggtgta aaaaaaatgt gtctggtgca cataggaaag aatctgcccg cagaaaaaaa
      601 agccgaattt ctggcaactc tgattgcaat gaaattaaaa ggtgaaatct gaaaaaaaga
      661 aagcctgcac actgtgcagg cctgagtgaa gaacctggga catttattca tcactcgcag
      721 taattttaat ctgagttgag gttaaaaaac aatgaacacc gataaacaag tttacccact
      781 gtattacgaa gcaaaaaatg acaaagtaag aaaacgtctc ggtattaaag gcggttttta
      841 ctgggctgaa gcgaaaaaat tatccattgc catctcccgt ggtgctgttg cgattgacga
      901 tgcaggctac gatgaagatg actttaaaaa acctgttcgc gtcaatttgc ccgttgttga
      961 tgacctcccg ccagaaggcg tatttgatac ggaattctgc aaccgttacg aaaaaggcgg
     1021 ggaagatggc atcacaatgg tatttatcgc atcttctccc tctgttcagg acaaaccggc
     1081 cagcactgac aataccaacg tcaacggcga agacatgaca gaaattgagg agaacatgct
     1141 cctgccagtt tccggtcagg aactgcccat tcgctggctt gcgcaacacg gcagcgaaaa
     1201 accggtaacg cacgtttcac gcgacgaact ccaggcatta cacattgctc gggatgaaga
     1261 actaccagct gttactgccc tagctgtttc ccacaaaacc agcctgctcg atccgctgga
     1321 aattcgcgat ctccacaaac tggtgcgtga tactgacaga gttttcccta atccaggcaa
     1381 ttcaagcctg gggctgatga ctgctttttt cgaagcatac atggacgcag actacaccga
     1441 tcgcggtctg ctgacaaaag agtggatgaa aggaaatcgt gtttcacgca tcacgcgcac
     1501 ggcttccggc gctaatgctg gcggcgggaa cctcaccgat cgcggcgaag gtttcgtcca
     1561 cgatctgacg tcactgacgc gcgatgtagc cactggcgta ctggcccgtt caatggacgt
     1621 ggacatctat aaccttcatc cggcacacgc taaacgcatt gaggaaat
//
LOCUS       DADLTF010000320         1655 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.326, whole genome shotgun sequence.
ACCESSION   DADLTF010000320 DADLTF010000000
VERSION     DADLTF010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.326"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(156..1391)
                     /locus_tag="JN749_004293"
     CDS             complement(156..1391)
                     /locus_tag="JN749_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HAY7370441.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="JN749_004294"
     tRNA            complement(1538..1614)
                     /locus_tag="JN749_004294"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
BASE COUNT          420 a          452 c          394 g          389 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtc
//
LOCUS       DADLTF010000321         1620 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.327, whole genome shotgun sequence.
ACCESSION   DADLTF010000321 DADLTF010000000
VERSION     DADLTF010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.327"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(822..1061)
                     /locus_tag="JN749_004295"
     CDS             complement(822..1061)
                     /locus_tag="JN749_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase"
                     /protein_id="HAY7370442.1"
                     /translation="MPQGGVISPLLSNIILNEFDQYLNKRYLSGKARKDRWYWNHSIQ
                     RGRSTAVKENWQWKPAVAYCCYADDCVPRRRVLGT"
     gene            complement(1256..1620)
                     /locus_tag="JN749_004296"
                     /pseudo
     CDS             complement(1256..1620)
                     /locus_tag="JN749_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          363 a          450 c          386 g          421 t
ORIGIN      
        1 ttcgtcaaaa aaatcatctt cgtgaagttt tacgacatag cggaggagtt tgcttctttg
       61 aggtgcaggt tttcgtttgc gtggtttagc tgaacgggcc atataactac ctgaaagaca
      121 atgatattgt ctgtttttat aacggtaatc acaggccatg acaagccgca gccgtcaggc
      181 cgcctactcg gggcggttta gaacgcttat ttagtaccag atcttactta taataaaggg
      241 gcttggcagt ttggataaac tattgtcctt attccactaa tacaaacttt ttgtaaagta
      301 tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac
      361 cataaaagga agagggggcc ccaattaagt tgtcaagcat gtttactgcg caatcgcttg
      421 gtgctccgtc cctgtacccc gcattggtac tctggcgctc atggggcttc cacttgcgct
      481 tctcccttca catcggggtg acaggtccac gttccccaca agagcctgaa tcgacttcac
      541 gccgctttta tgccggatgc cgcgcaggca gtaaacatat cccacaggaa cgactccacc
      601 attgttttga catcatctat atgctttcga cacatcatca gcggttcact ttcgttcgtc
      661 tcttcgaacc atacctgatc ggctcacgtc cgaccttttc ccatctcgct caccaccaca
      721 tctcttaaat gcagcagcag tggatggttt ggtgcctgcg cctgcacgcc gacaccgagg
      781 ggccttccct catctcttgt gctgcatggc tgcacaggga ctcacgtccc caggacgcgc
      841 cttcgtggca cacagtcatc ggcatagcaa cagtaagcaa cggcaggttt ccattgccag
      901 ttttctttga cagcagtact tcggcctcgc tggatactgt gattccagta ccagcgatcc
      961 tttctggctt ttccactcag gtagcgttta ttcagatact ggtcgaactc attcagtatg
     1021 atgttcgaca gcaacggcga gatcacaccg ccctgtggca tactttcact gaccgcccgg
     1081 aagagaccaa catcaacacg gcgttttcca cagcagtctc ataaaacgtg catcactgat
     1141 cctgcggcag acggctttca tcagcagccg gtgatgtact gtgtcgaagt agctggacag
     1201 gtcaccttca ataacccggc gccccgtgtt tccccgcagt cggtgagctg taatagtacg
     1261 tgatgtctcc cgcccacttc tggttcgggc cactggcgta aaaatcctgc gtcagcagat
     1321 tttctgatac aggcaggccg tgtgcacggt aactgatcgg gctgaacttc tgggaggctt
     1381 ttgcgctcag ttcctgacgg tgcagactgg ctgccacggt ttttacgtta aagtggtaac
     1441 cagagcacac agttcatccg tcaggcgtgg ggcaccgtaa cgctggactg ggtaaaagcc
     1501 ttgcggacaa cgctgtcgca gtgctgacgg aactgctgac gtgtgtttat ccttgtccgc
     1561 cgctgacacc acgtatacca gccgctgcgg gccacccgga gtacgcggca cattgccttg
//
LOCUS       DADLTF010000322         1577 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.328, whole genome shotgun sequence.
ACCESSION   DADLTF010000322 DADLTF010000000
VERSION     DADLTF010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.328"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..174)
                     /locus_tag="JN749_004297"
     CDS             complement(<1..174)
                     /locus_tag="JN749_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HAY7370443.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYP"
     gene            173..1312
                     /gene="queG"
                     /locus_tag="JN749_004298"
     CDS             173..1312
                     /gene="queG"
                     /locus_tag="JN749_004298"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAY7370444.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(1295..1348)
                     /locus_tag="JN749_004299"
     CDS             complement(1295..1348)
                     /locus_tag="JN749_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370445.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT          407 a          389 c          406 g          375 t
ORIGIN      
        1 aggatacgcc gaacgacaca cctggaatgc ggcctcgcca gcgcgaagca tcagctcata
       61 gagtgtgagc cccagcacat ctgccgcctc gcgttctccg cggcggatat cgtcggcgta
      121 ccagacggtg tgtggtatac ttacggggtt tttcttcatt gtatggtccg tcatgtcaga
      181 gcccctcgat ctcaatcagt tagcgcaaaa aattaaacag tgggggctgg aactgggctt
      241 tcagcaggta ggtattaccg ataccgatct cagcgagtcc gagcccaaac tgcaagcatg
      301 gctggacaaa caataccacg gcgaaatgga ctggatggca cgtcacggta tgctgcgcgc
      361 tcgcccccat gagttattac ccggtacgct gcgtgtaatc agcgtgcgga tgaattacct
      421 tcctgctaac gccgcatttg ccagcacgct gaaaaacccc aaactcggct atgttagccg
      481 ttatgcgctg ggccgtgact atcacaaact tctgcgcaac cgactcaaaa agctgggcga
      541 gatgattcag caacattgtg tttcgctgaa ttttagaccg tttgtcgatt ctgcgcctat
      601 tctcgagcgc ccgttagctg aaaaagctgg gctcggctgg acaggtaagc actcacttat
      661 cctcaatcgc gaggccggtt cgttcttctt tttaggcgaa ttgctggtcg atattccgct
      721 gcccgtggat caaccagtcg aggaaggatg cggcaaatgc gtggcctgta tgacgatttg
      781 cccgaccggt gccatcgtcg agccatatac cgtcgatgct cgccgctgta tctcttatct
      841 caccatcgaa cttgaagggg cgatcccgga agagttgcga ccgttaatgg gaaaccgtat
      901 ttacggttgc gatgactgcc agcttatctg cccgtggaat cgctattcgc aactcactac
      961 agaagacgat ttcagcccgc gtaagccgct acacgcaccg gaactcattg agttatttgc
     1021 ctggagcgaa gagaagtttt taaaagtcac ggaaggatcg gcgattcgcc gtataggtca
     1081 cctgcgctgg ctgcgtaata tcgccgtggc attaggcaat gccccttggg atgaaacgat
     1141 tttgacagcg ctggaaagtc gtaaaggtga gcacccactt ctcgatgagc acatagcgtg
     1201 ggcgattgcg cagcaaatag agagacgaaa tgcgtgcata gtcgaagtgc aattgccgaa
     1261 aaaacagcgt ctggttcggg tgattgaaaa agggttaccg cgtgacgcct gattcatcca
     1321 cagcttgtga ataaaaataa aaacgcattg caattcaaga cgaataaaat cgtcaagtga
     1381 tcgactcgac aaaacggaat cttgattttt aacttattta tcaataggtt aaaaaatacc
     1441 attcaccgtg cgaagtttct ttgaaattaa gattacagcc tgaggctgtg gataactctg
     1501 tttacaagag tttgtcagag acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat
     1561 attttattga gagaaga
//
LOCUS       DADLTF010000323         1576 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.329, whole genome shotgun sequence.
ACCESSION   DADLTF010000323 DADLTF010000000
VERSION     DADLTF010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.329"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..680)
                     /locus_tag="JN749_004300"
     CDS             complement(<1..680)
                     /locus_tag="JN749_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7370446.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
     gene            complement(996..>1576)
                     /locus_tag="JN749_004301"
     CDS             complement(996..>1576)
                     /locus_tag="JN749_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAY7370447.1"
                     /translation="VSNRLAWGCPTKEHLLPHFLEHLGNNHLDIGVGTGFYLTHVPES
                     SLISLMDLNEASLNAASTRAGESKIKHKISHDVFEPYPAALHGQFDSISMFYLLHCLP
                     GNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDS
                     EEGLTHILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          462 a          289 c          330 g          495 t
ORIGIN      
        1 aacaaagttt gcattattct gggagatagt tgattttctc taagattgac aacgctatca
       61 gaactgaggc caagtatgct ttccggaatt gccgtgatcc ggttctgatt aagccagaat
      121 attcttataa tatgatcatc tctttctgga aactctggta ttacttccaa agcatttctg
      181 gctgcatcaa gtaattccaa tgacattggt aacctcggaa gtacagacag gtgattgtca
      241 cttacattaa tatattctag tgacgcaggt aattcaggaa gtgcgaacaa atggttatca
      301 ctcacattta tatattccag cgacactggt aattcaggaa gtgcagataa ttgattactg
      361 cttgcattca gctctttcaa tgcccttggt agctcgggga gcattgatag ttggttattg
      421 cttacattaa tttcatcaag attgtcaggc aatcgtggta gagatctgag acctaaacaa
      481 gataagtcca acgatgtttc actgttctcc agacataatt ggagccgggt aaaagcagtt
      541 tccctgtttt ctccggaaat gctgttttta gtccattcaa cccattcgga gagataatta
      601 ttgtgaacat tgtcgattga tgtagttctg taaaaagaga cgtttccagt ggataggggg
      661 ggattattta caggaagcat aataacctcg cagaaggata tcctgataaa atgtgatact
      721 ggggaataat aagctgaaat aaaatgtttc aggattgaaa tttattgttt tcattttaag
      781 gaagtgatgt tgatttaact gtaaaaaaac gatactattt tcttagatgg taaatgtctc
      841 gcctgtgcta tattttgttt ttcggttagt taaatatcgt acgttcatat ttgaacgttc
      901 tgccggaatg cattatcaat agaggtaaag tcgcaacccc aaatcgtaaa ggaaaccgta
      961 gcacgtctta agcaagaacg tgctgcggtt ggatgctatt ttttccctga agcggaaaac
     1021 attactacag taccttgaac cttggtttta acattctcga aatgctctga gagtatatgt
     1081 gttaagcctt cttcggaatc ttttgtgttt gaaaagatgc ctttctgatt gtaaatgcgc
     1141 atcagttttt gaccgaagct attgtgcaca actccatcgc caagaattgt ggctccgtat
     1201 agagttccat catcagttaa ggcctgcgcc gcattgcgta ttacacagct ttttgtagat
     1261 atatttccag gcaggcagtg aagaaggtaa aacatggaaa tggaatcaaa ttgaccatgt
     1321 aacgccgcag gataaggttc aaaaacatca tggctaattt tatgtttaat ttttgattcc
     1381 ccagcccttg tagatgccgc gttcaggcta gcttcgttca aatccattaa agatatcaga
     1441 ctactctcag gtacgtgagt aaggtaaaac ccagttccaa caccaatatc cagatggttg
     1501 ttacctaaat gttccagaaa gtgtggaaga aggtgttcct ttgtaggaca tccccatgca
     1561 agccgatttg atactc
//
LOCUS       DADLTF010000324         1513 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.330, whole genome shotgun sequence.
ACCESSION   DADLTF010000324 DADLTF010000000
VERSION     DADLTF010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.330"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..823)
                     /locus_tag="JN749_004302"
     CDS             complement(<1..823)
                     /locus_tag="JN749_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7370448.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..>1513)
                     /locus_tag="JN749_004303"
     CDS             complement(1424..>1513)
                     /locus_tag="JN749_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HAY7370449.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          399 a          245 c          372 g          497 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tgattccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcg
//
LOCUS       DADLTF010000325         1500 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.331, whole genome shotgun sequence.
ACCESSION   DADLTF010000325 DADLTF010000000
VERSION     DADLTF010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.331"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..1071)
                     /locus_tag="JN749_004304"
     CDS             complement(<1..1071)
                     /locus_tag="JN749_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HAY7370450.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLS"
BASE COUNT          449 a          238 c          325 g          488 t
ORIGIN      
        1 ggaaaggcgg tcaaggaacg cggagaatgc ccgggaattc tcctcggtgg aaaaagaagt
       61 ccattgtgta gaaacctccc gacgatctgc ttcaggaaac caggctgcca cagtttcggg
      121 aagagggggg atctgaaagt tctgcccccc ttcagtgatg actctgatat ggcgcgaatg
      181 taaattttgc ggcaacatct ggcgtaaaaa cagtctgttt tggtctgaca gggaaatacg
      241 aaactgaggc ccgttaaaat ttggaaccga agtttgctgt attaagaata gcagagtttg
      301 atctgataag gggttatttg caatctcaat agttatatga ggatctaaac gaaaaagatt
      361 tgtcgggatt gtagttattt ggttgttttc aaaataaaaa aatcttctca tgataatatt
      421 ttcttggatg tttggtaagt tgcttaatct attgctatat gcaaaaagaa gttgcagtct
      481 cctaggtaaa gggggcagtt cagtaagatg gttgtgcgca accttaagta tcttaagatt
      541 agaaggcaga gatggaagag tttgcagttc gttgttccta acctcaagtt tttttaaatt
      601 caagggtaat gaaggcagtg tggataacct attatactct gcgcttagtg atttgatggt
      661 gtctggtaat aacggcagat tctctaattg attttcggat acatccaaag ttattagata
      721 atgtggtaat gcagatatat gtgttaagaa attatctctt gcaataagtt ctttaatatt
      781 ttcaggtaaa tcagtaagga tggttaattt attacagctc acatcaagct ttttaatgca
      841 agcagggagt acaggtaacg aggaaagcgc caaggctcgc aggtccagtt tatgcccctt
      901 agaggtaaga catgcacgaa gtcttcttac cgcctcagta cgctgttctt ccggtaaccc
      961 ctgtttctcc caatcatccc attcggacaa ataatggttg ggggatgtaa ttgtgctgat
     1021 atgtggaaat aaacaggatg aaatatttct gagcatatta atttctcgca tatggatatg
     1081 gaattagatt gtgaataaat tgtatttaat gtatctctcc taatgaggag ggtgtttcag
     1141 aatattcgcc ataagggaat gagtgttata tcatgctttc taccctggta agtatatttg
     1201 cttttttcgt atatttggtt catctttttt tgatgtttta catattcaca atgcagagga
     1261 tttataccag aagacattag tcttgagtaa gatactacaa catctccaac tattttagcc
     1321 agcctgacta cttgacttgg taatatttgt tgctaataat catgtataaa ataagcttat
     1381 atacatagta acttcagcaa aggatttagt aatgatccaa atcatatctc aatgtactgt
     1441 ttttttcttt aaaaaaacag tacattctat tttataatga ctgtgtattc ccatggaagg
//
LOCUS       DADLTF010000326         1492 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.332, whole genome shotgun sequence.
ACCESSION   DADLTF010000326 DADLTF010000000
VERSION     DADLTF010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.332"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            67..696
                     /locus_tag="JN749_004305"
     CDS             67..696
                     /locus_tag="JN749_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HAY7370451.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(835..>1492)
                     /locus_tag="JN749_004306"
     CDS             complement(835..>1492)
                     /locus_tag="JN749_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HAY7370452.1"
                     /translation="YYFSYVIGDADLFPYYLSYAGAANLVTLVFFPRLVKSLSRRILW
                     AGASILPVLSCGVLLLMALMSYHNVVLIVIAGILLNVGTALFWVLQVIMVADTVDYGE
                     YKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLGMIGYVPNVEQSTQALLGMQFIMI
                     ALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA
                     "
BASE COUNT          391 a          377 c          366 g          358 t
ORIGIN      
        1 ttttgcgatt gtaatttcat gcttgctggt gaatagtcag tattttccct gacttgcgaa
       61 ctcaccatga cccgcacact caagccgtta attcttaaca ccggcgcact ggcgctaacg
      121 ttaatcctga tttataccgg catttcggcc catgacaaac tcacctggct gctggaagtg
      181 acaccggtga ttatcgtcgt gccgctacta cttgccaccg ccagacgtta cccgttaacg
      241 ctgctgctct atacgctcat cttctttcac gccatcatcc tgatggtagg cgggcagtac
      301 acctacgcga aagtccccgt tggttttgag gtgcaggaat ggctggggtt gagtcgtaat
      361 ccgtatgaca agctggggca ctttttccag gggctggtgc ctgcactggt ggcacgagaa
      421 attctcgttc gcgggatgta cgtgcgcgga cgtaaaatgg tggcgtttct ggtctgttgc
      481 gtggcgctgg cgataagcgc gatgtatgaa ttaatcgagt ggtgggcggc actggcgatg
      541 ggccagggag cggatgattt tctcggtacc cagggcgacc agtgggacac gcaatccgat
      601 atgttctgcg cgctgcttgg cgcattaacg acagtgatat tgctcgctcg ctttcattgc
      661 cgccagctac ggcgctatgg cttgataacc ggataacaac gtgcactttg ttgatgccgg
      721 atgcggcgtg aattccttat ccggtctaca aaatttgcaa atccaaagca ttacattaag
      781 acgtggcctg ataaacatat cgcatcaggc cattttcgtt agctattgtc cacgtcaggc
      841 tttcacatca ctcactgcac cgaccggaat atcagcatga acaggctcgg gcggtacttt
      901 gcgatattta tccagcagat gaatctggat cctgcgcagc gtgtcgccat tgaggcgata
      961 gaaacggaag tagagaatca gcgttaccat gaaaaacaga gtcggtagag caatcataat
     1021 aaactgcata cctaataggg cttgcgtaga ctgttcaaca ttcggtacat agccaatcat
     1081 ccctaacaca accgcaatga aaaaagccgc aaaggctgaa ccgcccttca ccaccatagt
     1141 ctgcacggag taagcgatgc tttcacagcg tacgtgcagt ttatattcac cgtaatcaac
     1201 ggtatctgcc accatgatga cctgtaatac ccagaaaagc gccgttccca cattcagcaa
     1261 aatacccgca atcacaatga ggacgacgtt gtgatagctc attaatgcca ttaacaggag
     1321 aacaccacag cttaacaccg gaagaataga tgctccggcc cataaaatgc gtcgggataa
     1381 tgatttaact aagcgtggga agaatactaa cgtcaccagg ttagcagctc ccgcatacga
     1441 cagataatag gggaacaaat ccgcatcacc gataacatat gagaaataat ag
//
LOCUS       DADLTF010000327         1489 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.333, whole genome shotgun sequence.
ACCESSION   DADLTF010000327 DADLTF010000000
VERSION     DADLTF010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.333"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(195..347)
                     /locus_tag="JN749_004307"
     CDS             complement(195..347)
                     /locus_tag="JN749_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370453.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            365..556
                     /locus_tag="JN749_004308"
     CDS             365..556
                     /locus_tag="JN749_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HAY7370454.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            867..1385
                     /locus_tag="JN749_004309"
     CDS             867..1385
                     /locus_tag="JN749_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HAY7370455.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
BASE COUNT          432 a          326 c          281 g          450 t
ORIGIN      
        1 cattcagttt catagatgct cagcagaatc ccccacatcc tgaaggaggt gtattcagac
       61 aggcatccca cctgacttcg aatgatgatt attcatcact atagagagca ttgattctaa
      121 gtgtcatatg aaagttccaa ttgatatata tcaaacaaaa taaccctgat taatgaatta
      181 ttacgtttat catgttaatt catcattatt acatcatcat tgtaaataat taaattaact
      241 tccataacat taaaatatgt atccactgac gcttttttac ataacgaaga attgaccatt
      301 ttgtcctgtt gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc
      361 ttttgtgagt caggcaacta gtatgcgaaa acgacaccga tttaacagtc gcatgacccg
      421 tatcgtactg ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg
      481 tgcctggctg caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc
      541 accggtacaa cgttagcggt taccttctcc actttcacag aacataacgg cacttcgctg
      601 tcggatgctt ttgctctttg ggattatcaa agcggcagat attctttcat cttaaatttt
      661 acgtctttgt cctgactgat gtttatcctg tttggctgcg aaataaatat aaaattaata
      721 tatatgttgt aatgatatat ttttataaat tattccctgc gtgaatttta ataaatttaa
      781 tctatccctt tatacgcaat acatttactt tcctcttttg atgatcttaa atgtcttatt
      841 tttcgtaatg tgtataacaa ggaatagtga tgaaatttaa aaaatgtctt ctgcctgtgg
      901 caatgttagc gtcattcact ctggcaggat gccagtcaaa tgctgacgat catgccgccg
      961 atgtttatca aaccgatcaa ctgaatacca aacaagaaac taaaaccgtt aatattattt
     1021 ccattcttcc cgcaaaagtt gccgtagaca actcccaaaa taaacggaac gcacaagcct
     1081 tcggcgcgct tattggcgca gtcgctggcg gtgttatcgg ccacaacgtc ggttctggca
     1141 gcaattccgg aacgacggca ggggcagttg gcggcggagc tgtaggcgcg gcagcgggtt
     1201 ctatggtgaa tgataaaacc ttagtggaag gtgtttcttt aacatataag gaaggcacca
     1261 aagtgtatac ctccacccag gagggtaaag agtgccagtt tacgacaggt ttagccgttg
     1321 ttattaccac aacgtataac gaaacgcgta ttcagccaaa taccaaatgt cctgaaaaga
     1381 gctaataatc aggaggagtc atgaagaaag tttttctttg cgccatctta gcctccttaa
     1441 gctatccggc tatagcctca tcattgcagg atcaactctc tgctgtcgc
//
LOCUS       DADLTF010000328         1484 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.334, whole genome shotgun sequence.
ACCESSION   DADLTF010000328 DADLTF010000000
VERSION     DADLTF010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.334"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(133..810)
                     /locus_tag="JN749_004310"
     CDS             complement(133..810)
                     /locus_tag="JN749_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370456.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            complement(946..>1484)
                     /locus_tag="JN749_004311"
     CDS             complement(946..>1484)
                     /locus_tag="JN749_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HAY7370457.1"
                     /translation="GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFG
                     DGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLK
                     YAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVLQKSL
                     EITEEADLALRNAMARIK"
BASE COUNT          360 a          382 c          340 g          402 t
ORIGIN      
        1 cgcctctgta gtattaacgt atttgatgtt gtttggcagt tttacttgtt tcgccgtagc
       61 gaagacagag aacagaatag ttacagcaac gagtaaagtt ttgataaaag ttttcatgac
      121 agactcctta aattagttaa ccgcagctac gtagtttttc accagatacg cgaattcatg
      181 cacgccaatt tcgccgaacc aaaccaggta ggccgggtca tctaaaccaa agtagcggta
      241 gccaacgact tcatgggttt ttgggttata gagtgttacg ataaagtccc agccgcaggt
      301 attggctttg caaccgtcgc cctggagcca gactgtaccg ttaatggtta ctggcgttac
      361 gtttccgcca gggccatcca tatacccgcc tttaccgaac atgccgctca gtccgaccgg
      421 ttttaatgct ttgtcaagcg cagctttgta ggcagcgtca ccttcgtaca actcgtacgt
      481 tgttggacac gctccaggtt gttcagcccg ggcgtttggg tcgcagctga gcgcttgttt
      541 cacgctcgct gcttccacgt taaagccagt aaacagaacc gccagaaata acatcagggt
      601 atgtaaacgt ttcattgtaa atattcttcc gttgtaaatg gcgctggaaa atttctcatc
      661 agtgaaacct tccggaaatt tccccccctt gtatgatgta tatcgccagt attttatttc
      721 atcccggata taatttttat atttcccggc acgtgcaata acgctgacca gagatataag
      781 gctcattaat aactttacag gtaattgcat aattaaattt ccagtgagtg aattgacagg
      841 tgaggggtaa aactttctct caggtaatat atatctgaaa atattttcaa taacaccagc
      901 taggaaaaac accaccacag gcggtggcgt ttctttggag cgtctttatt taatccgggc
      961 catcgcgttt ctcagtgcca aatctgcctc ttccgttatc tccagacttt tctgcaacac
     1021 cgtttctgac cgggacagta cctgacgtgc tgccagcggt ttccggatat gctccatcgt
     1081 cagcatcgtg tcttcatagc cctgctgacg ccaggcgtcg gtatgtgcca acgtgaaact
     1141 gagcagaccg ccgctgttgt caccatcacc tgcatatttc agccttttcc ggggacggat
     1201 ctcaaggatt gtggtgtccg gaaaagcctg gcgatcccat aaagaaccgt cactcagatg
     1261 cgtcacaatc accatattgc atccggcatc gaccagaggc gtcacagggg tatttccctg
     1321 catatttcgc catcctccca taccaccatc accgaacatt gtcccctgaa cctcacgggg
     1381 acggaacagc aggggcagcg cggctgacgc aagcagagcc tctttctgct gtccgcgggg
     1441 caggctctgg atatgctgaa aaacggcgtt tttcgtggtt ccgg
//
LOCUS       DADLTF010000329         1467 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.335, whole genome shotgun sequence.
ACCESSION   DADLTF010000329 DADLTF010000000
VERSION     DADLTF010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.335"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            184..402
                     /locus_tag="JN749_004312"
     CDS             184..402
                     /locus_tag="JN749_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370458.1"
                     /translation="MKAVARANNHPYKSVFADFITGHPSCTVCFWETFHKMYPDSPYE
                     YVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            383..>1467
                     /locus_tag="JN749_004313"
     CDS             383..>1467
                     /locus_tag="JN749_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7370459.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSH
                     ITTESQRRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSR"
BASE COUNT          353 a          381 c          439 g          294 t
ORIGIN      
        1 tgatacgccg gagccgacca gaccagttac aaatcccttc tggcgtaaga accacggcag
       61 cctggcggct gcaaatcggg gtgtggcaga atatgagctt tcagagtaac catccagaat
      121 gagttcccat gtcacacaac ctggagcacc agaaagtcca caccagaatg gtgaaagagg
      181 gttctgaaag ccgtcgccag agccaataat cacccttata agtccgtatt tgcagacttt
      241 atcaccggtc atccctcctg cacggtctgt ttctgggaaa ccttccacaa aatgtacccg
      301 gattcaccgt acgaatacgt cacgttctgc catacctgcc gtcgttttga tttatacgaa
      361 acagaagcgg agatgaaggc ggatgacccg aagtggtggt gattttcagc cccgcccgct
      421 gaaacgcctg ttcacagcca accagtgctg gacgtccttc ctggatgcgg gcggtctgcg
      481 cgatatcgag gttgaggctg tcaccaaaat gctggcctgc ggcacgcgga tactgggcgt
      541 gaaggaatac atctgcgata aacctgagtg cccccacgtc agatacgtca ctaactcatg
      601 cggcagccgt gcctgcccgt cctgcggaaa aaaggccaca gacctgtgga tagcgacaca
      661 gctgaatcgt cttcctgact gcgactgggt acacctggtc ttcaccctgc cggacacgct
      721 gtggccggtg ttcgaaagca accggtggct gctgaatgac gtgtgccgtc tggcggtgga
      781 gaatctgctg tatgccgccc ggaaacgggg gcaggaaccc ggtatcttct gcgccatcca
      841 cacgtatggc cgtcgtctca actggcatcc gcatgtacat gtgtctgtaa cctgtggagg
      901 tctgaataag catggtcagt ggaaaaagct gagcttcctg aaagacgcga tgcgttcgcg
      961 gtggatgtgg aatatgcggc aacggcttct gaaagcgtgg tcagaggggc tggcgatgcc
     1021 ggagtcgttg tcacatatca cgacggaatc acagaggaga agcctggtgc tgaaagccgg
     1081 aggaaaatac tggcatgtgt acatgtcgaa aaaaacggcc ggcgggagga atacggcgcg
     1141 ctacctgggt cgttatctga agaagccgcc gatagcggcc tcccggctgg cacattacaa
     1201 cggaggggcg agcctgagct tccgttacct ggaccacaaa acgggagaaa cggcgacgga
     1261 aacgctgaca cagcgtgagc tggtcgcgag gctgaaacag cacatcccgg agaagttttt
     1321 taagatggtg aggtacttcg ggttccttgc cagccgggtg tgtggagaga agctgccgca
     1381 ggtgtaccgt gctctgggga tggataaacc ggaaccagtg gcgaaagtgt gctatgcaca
     1441 aatggtgaaa cagttcctga gtcgtga
//
LOCUS       DADLTF010000330         1438 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.336, whole genome shotgun sequence.
ACCESSION   DADLTF010000330 DADLTF010000000
VERSION     DADLTF010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.336"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1059
                     /locus_tag="JN749_004314"
     rRNA            <1..1059
                     /locus_tag="JN749_004314"
                     /product="16S ribosomal RNA"
     gene            1116..1192
                     /locus_tag="JN749_004315"
     tRNA            1116..1192
                     /locus_tag="JN749_004315"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1150..1152,aa:Ile,seq:gat)
     gene            1235..1310
                     /locus_tag="JN749_004316"
     tRNA            1235..1310
                     /locus_tag="JN749_004316"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Ala,seq:tgc)
BASE COUNT          368 a          326 c          426 g          318 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac ctccttacct taaagaagcg ttctttgtag tgctcacaca
     1081 gattgtctga tgaaaatgag cagtaaaacc tctacaggct tgtagctcag gtggttagag
     1141 cgcacccctg ataagggtga ggtcggtggt tcaagtccac tcaggcctac caaatttgca
     1201 cggcaaattt gaagaggttt taactacatg ttatggggct atagctcagc tgggagagcg
     1261 cctgctttgc acgcaggagg tctgcggttc gatcccgcat agctccacca tctctgtagt
     1321 gattaaataa aaaatacttc agagtgtacc tgcaaaggtt cactgcgaag ttttgctctt
     1381 taaaaatctg gatcaagctg aaaattgaaa cactgaacaa cgagagttgt tcgtgagt
//
LOCUS       DADLTF010000331         1426 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.337, whole genome shotgun sequence.
ACCESSION   DADLTF010000331 DADLTF010000000
VERSION     DADLTF010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.337"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(11..202)
                     /locus_tag="JN749_004317"
     CDS             complement(11..202)
                     /locus_tag="JN749_004317"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370460.1"
                     /translation="MALPFAADACLVASSFEADADDADDAAEPADVLAARAEPAASVA
                     LVAASLADVPASLAEVYWQ"
     gene            339..>1426
                     /locus_tag="JN749_004318"
     CDS             339..>1426
                     /locus_tag="JN749_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370461.1"
                     /translation="MEDASTTKKGIVQLSSATNSASESLAATAKAVKVVMDETNKKAP
                     LNSPALTGTPTTPTAPKGTNNTQIASTAYVMAAIAALVDSSPDALNTLNELAAALGND
                     PNFATTMTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAK
                     STVAAVIEYLGLQETVNKADNAVQKTGDTLSGGLTFENDSILAWIRNTDWAKIGFKND
                     SDADTDSYMWFETGDNGNEYFKWRHRLAGGQLKELMNLKWDSLNILVNAVINGCLGIG
                     TTNALGGNSIAFGDNDTGLKQNGDGLLDVYANGQHVFRFQNGVAIAFKNIQAGTARKF
                     TLSSANNSTKNAAFYLWGNPSRPVVAELG"
BASE COUNT          371 a          354 c          405 g          296 t
ORIGIN      
        1 gtagtgtccg ctattgccag tacacctcag ccagggatgc cggcacatct gccagtgagg
       61 cggcaaccaa tgcgactgat gctgcaggct cagcacgcgc agccagcacg tcagccggtt
      121 cggcagcgtc atcggcatca tctgcgtctg cttcaaaaga tgaggcgact aaacaggcgt
      181 cagcagcgaa gggcagtgcc acgacggcat cctctaaggc gacagagtct gcaggcagtg
      241 cgacggcggc agcgcagagc aaaagcgcgg cagaatccgc ggcaatgcgc gctgagacag
      301 cagcaaaacg ggcagaggat attgctgatg tcgtatctct ggaagatgcg agcacgacga
      361 aaaaggggat agtgcagctc agcagtgcga ccaacagtgc atccgagtca ctggccgcga
      421 cagcgaaagc ggttaaggtg gtaatggatg aaacaaacaa gaaagcgccc ctgaacagtc
      481 cggctctgac cggaacgcca accacaccca ctgcgccaaa ggggactaat aatactcaga
      541 tcgccagtac ggcttatgtt atggccgcga ttgctgccct cgtggactcg tcgcctgatg
      601 cactgaacac gctgaatgaa ctggcggcgg cgctgggcaa cgacccgaat tttgcgacca
      661 ccatgactaa cgcgcttgcg ggtaagcaac cgaaagatgc cactctgacg gcgctggcgg
      721 ggcttgctac tgcggcagac aggtttccgt attttacggg gaatgatgtc gccagcctgg
      781 caaccctgac aaaagtcggg cgggatattc ttgcgaaatc gaccgttgcc gccgttatcg
      841 aatacctcgg tttacaggaa acggtaaaca aggctgataa cgctgtccaa aagacaggcg
      901 ataccttgtc cggtggactt acttttgaaa acgactcaat ccttgcctgg attcggaata
      961 ctgactgggc gaagattgga tttaaaaatg attcggatgc agacaccgat tcctacatgt
     1021 ggtttgaaac aggtgacaac ggcaatgagt atttcaaatg gagacaccgc ctcgctggcg
     1081 gccagctcaa agagctgatg aatcttaaat gggattcact aaatattctg gttaatgccg
     1141 tcattaatgg ttgccttggc attggtacga cgaatgcgtt aggtggaaat tcaattgctt
     1201 tcggggataa cgataccggc ctgaaacaga acggcgatgg tctgctggat gtttatgcga
     1261 atggacagca tgtatttcgt tttcagaatg gcgtggctat tgcttttaag aatattcagg
     1321 ccggaactgc cagaaaattc acgttatcca gcgccaacaa ctccacgaaa aatgcagcgt
     1381 tttatttgtg gggtaatcca tccaggcctg ttgttgcaga gcttgg
//
LOCUS       DADLTF010000332         1419 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.338, whole genome shotgun sequence.
ACCESSION   DADLTF010000332 DADLTF010000000
VERSION     DADLTF010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.338"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            464..1150
                     /locus_tag="JN749_004319"
     CDS             464..1150
                     /locus_tag="JN749_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HAY7370462.1"
                     /translation="MSELTAKALSDSRSFENAILERDMRMKKAGWIVAALAGLLLVFA
                     LAALILLLPLKTTTVELYTLDRQTGRIERVTTVGEDKLDTSEVLNLSQTASYVKRREG
                     YNYFALQQDYRETQMFNSDEVNKEYLDWFNSPDAPDAVFQQAAYVVTVEVLSNVPSEG
                     TGSSMVEMLRIKRTIRKVKDNTETYDYWTVRMTYRYFPQKKMTASEREVNPFGFIVTS
                     YQRFKEKSDE"
     gene            1143..>1419
                     /locus_tag="JN749_004320"
     CDS             1143..>1419
                     /locus_tag="JN749_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HAY7370463.1"
                     /translation="MNKVLMCALLLTAASAWGAATPRGSAYDSRMQNIPYNSQNTTVI
                     NTRAGYITTLVFDADEEVITATAGFPQGWTITPDRNRVGISPAPVKQP"
BASE COUNT          305 a          461 c          412 g          241 t
ORIGIN      
        1 ataagctggc cgaggcgctt gccggtgcat ccgcacaggg tgccgctcag gcacttgccg
       61 cgtcgggagt aggttcagcc cgtcaatccg cctcctccgc agccggtgcg gtgggggata
      121 aagcctcaca gaaccttaac agcaccggca gcgcaacggc ctcgtctgcc tccacggcct
      181 cccgcgccac cggcgatgcg cgcagccgca gccgcgccgc cgttcagcgg atgcaggaac
      241 gctaccagag ataacccagt ctgactgaac tgcgggacgc cgcaacggcg tcctgcggct
      301 tcgtcacgtc tttaccacag gaaaaacacc atgaaatacg gcatcacgct gctgatgctg
      361 tgcctgcttg caggctgcgc acagcgaaac acccaacttc cgcccgtgtc gggcaacccg
      421 gagccggtca attccccggc cattattcag gagttaaccc cccatgtctg aacttaccgc
      481 caaagccctg agcgactcgc gctcgtttga gaacgccatt cttgagcggg atatgcgcat
      541 gaaaaaggcg ggctggatcg tggcggcgct cgccggtctg ctgctcgtct tcgcccttgc
      601 cgcccttatc ctgctgctac cgctcaaaac caccaccgtg gagctgtaca cgctcgaccg
      661 tcagaccggg cgcatcgagc gcgtcaccac cgtcggcgag gacaagctgg acacctccga
      721 ggtgctcaat ctctcgcaga ctgcgtccta cgtgaagcgc cgcgaggggt acaactattt
      781 cgcccttcag caggactaca gagagacgca gatgttcaac agcgacgagg tcaacaagga
      841 atacctcgac tggtttaaca gcccggacgc gccggacgct gtgtttcagc aggcggccta
      901 tgtagtcacg gtggaagtcc tctccaacgt accgtcagag ggcaccggct ccagcatggt
      961 ggagatgctg cgcatcaagc gcaccattcg aaaggtcaag gacaacaccg aaacgtatga
     1021 ttactggacg gtgcgcatga cctaccgcta tttcccgcag aagaaaatga cggcctccga
     1081 gcgcgaggtg aacccgttcg gcttcatcgt caccagttac cagcggttta aggagaaaag
     1141 cgatgaataa ggtactgatg tgtgccctcc tgctgaccgc agcgtcagca tggggggcgg
     1201 cgacaccgcg cggcagcgcc tatgacagcc gcatgcagaa catcccctac aacagccaga
     1261 acacgacagt gattaacacg cgggcgggtt acatcacgac gctggttttt gacgccgacg
     1321 aggaggtgat caccgccacc gctggttttc cgcagggctg gacgatcaca cccgaccgta
     1381 accgcgtggg gattagcccc gcgccggtga agcagccgg
//
LOCUS       DADLTF010000333         1411 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.339, whole genome shotgun sequence.
ACCESSION   DADLTF010000333 DADLTF010000000
VERSION     DADLTF010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.339"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..680)
                     /locus_tag="JN749_004321"
     CDS             complement(<1..680)
                     /locus_tag="JN749_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAY7370464.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLT"
     gene            777..>1411
                     /locus_tag="JN749_004322"
     CDS             777..>1411
                     /locus_tag="JN749_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAY7370465.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT          407 a          288 c          336 g          380 t
ORIGIN      
        1 ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg catttgctga
       61 acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag tgtacggcta
      121 ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg gttgtttagc
      181 aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc aagtgacatt
      241 ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa acgtaactgt
      301 tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag taattccctt
      361 aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact ttccgcttct
      421 gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg cggcatttgt
      481 ggcggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc agcccataca
      541 cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg ccagggaact
      601 gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg tgatgctttg
      661 ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca aataagccat
      721 gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa agagtgatga
      781 taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg actggcgttg
      841 tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat gatgcttaca
      901 ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct gctatagagg
      961 tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat gatcgggatt
     1021 atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc aatctcgaag
     1081 gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt gaaacatggc
     1141 aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat gaaaaactcg
     1201 cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac taccagaagg
     1261 tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa cggctagcag
     1321 ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag gccgctttga
     1381 ctcaggcact actggatctg gaatataccc t
//
LOCUS       DADLTF010000334         1389 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.340, whole genome shotgun sequence.
ACCESSION   DADLTF010000334 DADLTF010000000
VERSION     DADLTF010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.340"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..572)
                     /locus_tag="JN749_004323"
                     /pseudo
     CDS             complement(1..572)
                     /locus_tag="JN749_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
     gene            complement(620..1114)
                     /locus_tag="JN749_004324"
     CDS             complement(620..1114)
                     /locus_tag="JN749_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7370466.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          423 a          280 c          263 g          423 t
ORIGIN      
        1 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
       61 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      121 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      181 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      241 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      301 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      361 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      421 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      481 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      541 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaattgaa
      601 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      661 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      721 ccttgccttc agcatcgata gcggtataat cagagtcgtt ataaagttta acctgcgaat
      781 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      841 catcaaaacg aacggctgct tgagtgtacg ttgccgggca attttcaagt ttaatactga
      901 acttctgtgg cgacgccaga ctacctacac ctgaaaatgc atttttactg actttaccca
      961 gtgggacaat ttgatcggct gtatcggagg taacttcaca agtagtgtcg acaatttcac
     1021 cgacgaagtt aacaacacca tcttctgcca gtgcatgccc ggccagcatt agagatgcca
     1081 gagtgaatgc accgcctata atattacgct tcataaaaac tccattgata taatatatcc
     1141 tttaccatga atcatcactt atattaataa gtgtattttt gattcaaagg aatagtacac
     1201 caaagagacg gctttatcta aaaataatat taattacgca aaaataaaat atagatttat
     1261 tgtttaaata ttatttaaac agtaaaaaat caagtttgtt atcagacatt aatgataatt
     1321 cagaatatat ttatatagaa agatagatga atttattgat gtcagttata aaaaagggcc
     1381 accggtcgg
//
LOCUS       DADLTF010000335         1355 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.341, whole genome shotgun sequence.
ACCESSION   DADLTF010000335 DADLTF010000000
VERSION     DADLTF010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.341"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            193..462
                     /locus_tag="JN749_004325"
     CDS             193..462
                     /locus_tag="JN749_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370467.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..571
                     /locus_tag="JN749_004326"
                     /pseudo
     CDS             459..571
                     /locus_tag="JN749_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970002.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            714..1304
                     /gene="ospG"
                     /locus_tag="JN749_004327"
     CDS             714..1304
                     /gene="ospG"
                     /locus_tag="JN749_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HAY7370468.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          389 a          262 c          333 g          371 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt gaaaatctct tgtgattttt aaatatcgta acaaaggtat gacatgaaaa
      721 taacatctac cattattcaa acaccttttc catttgagaa taataattct catgctggca
      781 tagtaacgga gcccattctc ggtaagttaa taggtcaggg gtcgacagca gaaatctttg
      841 aagatgtgaa tgattcatct gctttgtata aaaagtatga tcttattggc aaccagtaca
      901 atgagattct ggaaatggct tggcaagaat ctgagctttt taatgctttt tatggcgatg
      961 aagcatccgt tgttatacag tatggcggag atgtgtacct ccgaatgctg cgcgtgcctg
     1021 ggactcccct tagtgacatt gatacagctg atatccctga taatatagag agcctttatc
     1081 tacagttgat atgtaaattg aatgagttga gtataatcca ttacgatctt aatacaggta
     1141 atatgctgta tgataaagaa agtgaaagtt tattcccaat agattttcgc aatatttatg
     1201 ctgaatatta cgctgcaacc aaaaaagata aagagattat cgaccgacga ttacaaatgc
     1261 gtacaaatga tttttattcg ttattaaaca ggaaatattt atagccgtat ttgttgatgc
     1321 tataagccag aggctcttct atgagaatat gggga
//
LOCUS       DADLTF010000336         1322 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.342, whole genome shotgun sequence.
ACCESSION   DADLTF010000336 DADLTF010000000
VERSION     DADLTF010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.342"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JN749_004328"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="JN749_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HAY7370469.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPIFTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          371 a          241 c          296 g          414 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta aaaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc agggccagta cctcgtcagt
     1321 ca
//
LOCUS       DADLTF010000337         1297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.343, whole genome shotgun sequence.
ACCESSION   DADLTF010000337 DADLTF010000000
VERSION     DADLTF010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.343"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(86..364)
                     /locus_tag="JN749_004329"
     CDS             complement(86..364)
                     /locus_tag="JN749_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370470.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(431..681)
                     /locus_tag="JN749_004330"
                     /pseudo
     CDS             complement(431..681)
                     /locus_tag="JN749_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(899..1054)
                     /gene="hokD"
                     /locus_tag="JN749_004331"
     CDS             complement(899..1054)
                     /gene="hokD"
                     /locus_tag="JN749_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HAY7370471.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1126..>1297)
                     /gene="relE"
                     /locus_tag="JN749_004332"
     CDS             complement(1126..>1297)
                     /gene="relE"
                     /locus_tag="JN749_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HAY7370472.1"
                     /translation="EANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRER
                     SEVYSEAVKRIL"
BASE COUNT          323 a          332 c          307 g          335 t
ORIGIN      
        1 gaataccggc atggattcac gaccgggctt aaggaccacc agcccgagtt ccggaaccag
       61 aacaggtcga agtaataccc gcacgttacc tccagatccg ttgctggaat gtgcgggacg
      121 gacgcggtgg ctgttcggag taagggagcc tgacggagat tatccagtga cggtagtcga
      181 ggctaagggc ttttttaacc tcgcatccgc gcctgcggta acactgaatg agccattcgg
      241 cctgttcttc agtgcatgag ggatgctggt accagtctga cttaaatgcg tgagaatacc
      301 gctcgtgcgt gtgggcaaga acggtcgaat tatcatgatt gtaatatttt gcgttgcgtg
      361 ccatcggttt tctccggtgg cacggtgtta ctcagcggga gttcagcccc gcgcaagatt
      421 gtagatgagt ttattcttct gaaaaagcag aaaagccagc ttttattccg atctctttca
      481 atgcctgtaa tgaagtgaca aactcaccgt cgcgcaagat aaatccgtcc gtcactcggg
      541 catccacaaa attaattaac gcagccccat tttttgcaaa cacacaatgc ggtaatgact
      601 aacaatattt ccattttcaa cgcacacagc atagaggcca tcttcacaaa aaattttacg
      661 cagttcttcg atgttcatca tcagaatcct tccagataag tagcactccc ctgttcgggg
      721 tccatccctt ttatccctgc gcgctactta agtatttttg attctattcc ggcaccatcc
      781 agaacttcaa atgcgttgaa aataaaaaca aaaacccgcc gaagcgggtt aagtgcgggt
      841 gcgttgagga tgcctgccac atcagaggtg gcgagggatt tctcactcgc cgggtctctt
      901 actcctcagg ttcgtaagct gtgaagacag cgacctccgt ctggccggtt cggattcgta
      961 cctcgcagag gtctttcctc gttaccagtg ccgtcactat gacggttaaa cagatgacga
     1021 tcagggcgat taacatcgcc ttttgctgct tcatagcctg cttctcctgt caacgcaaag
     1081 cagaagtgtc accttcggtg cgaaacagag atgtcatgct ttggttcaga gaatgcgttt
     1141 gaccgcctcg ctatatactt ccgagcgttc tcttttccca acagaaatca cgaaaacgac
     1201 aactttctcg tctataacct ggtatacaag gcgatagcct gaagaccgga gcttaatctt
     1261 gtaacaatca ggcataccac ggagcttgtt tgcttca
//
LOCUS       DADLTF010000338         1297 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.344, whole genome shotgun sequence.
ACCESSION   DADLTF010000338 DADLTF010000000
VERSION     DADLTF010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.344"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..123)
                     /locus_tag="JN749_004333"
     CDS             complement(<1..123)
                     /locus_tag="JN749_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370473.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYA"
     gene            181..1206
                     /locus_tag="JN749_004334"
                     /pseudo
     CDS             181..1206
                     /locus_tag="JN749_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            1179..>1297
                     /locus_tag="JN749_004335"
     CDS             1179..>1297
                     /locus_tag="JN749_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HAY7370474.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQV"
BASE COUNT          361 a          316 c          301 g          319 t
ORIGIN      
        1 ggcataaagg ctgtgggtgg tgatatcgag acgcgttgca acgctggcaa cagaataacc
       61 gcgatcaaca acctgtttga ctgcttcagt tttaaactct tcgggataac gcttaccgct
      121 catgggcacc tctctttaag ccatcttaaa tgactctgag gtgtctgtta aacccgtggc
      181 gattcacaat tctcttgcac cattaactat gttcagtccc tgcgcgagtt gtacaccgat
      241 gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc cctttcacaa
      301 taccaacccg aatggcagtg ctgagaacct gatgtcgaat gggtgcgtca gcccccgcaa
      361 gatgcgataa ccgctcctga ctttttctgc ttttaccagc caggcatgac cttcgaacaa
      421 tttgtacgtg agtttgccga atggttttca caaaaacgtc cggcggcgat gatgatcggc
      481 atccgtgcgg atgagtccta caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt
      541 tttgccgacg ataaaccatg gactacggcc gcaccaggcg gtcatagctg gtacatttac
      601 cccatttacg actggaaagt ggctgatatc tggacctgat atgcaaatca tcaaaatctc
      661 tgcaacccac tgtataacct aatgtatcag gcaggcgttc ctctgcgtca tatgcgaatt
      721 tgcgaacctt ttggcccgga gcaacgacaa ggattatggc tctatcacgt tatcgaaccg
      781 gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg catttacgcc
      841 ggacatgaca atcatttcta tgggcatcgg aaaatcctca aaccagaaca tttagactgg
      901 caagaatatg cattattgct gctcaatagc atgccggaaa aaacagctga gcattaccgc
      961 aataaaatcg ccatttattt gcactggtat cagaaaaaag gcatcgagat tccacaaacc
     1021 cagcaagggg acattggcgc gaaagatatt ccctcctggc ggcggatatg caaagtttta
     1081 ctcaataacg attactggtg tcgggcatta tcatttagtc ctacgaaagc gaagaactat
     1141 cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact atgcaacaac
     1201 gattaacgca ggatttaacg caatttctcg ccagcttgcc ggaagatgat cgcatcaaag
     1261 cgattaatga aatccgcatg gcgatccatc aggttag
//
LOCUS       DADLTF010000339         1296 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.345, whole genome shotgun sequence.
ACCESSION   DADLTF010000339 DADLTF010000000
VERSION     DADLTF010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.345"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..815)
                     /locus_tag="JN749_004336"
     CDS             complement(<1..815)
                     /locus_tag="JN749_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7370475.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="JN749_004337"
                     /pseudo
     CDS             907..993
                     /locus_tag="JN749_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1003..1083)
                     /locus_tag="JN749_004338"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="JN749_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          361 a          218 c          338 g          379 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gactaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaagtg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tgtgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctt
//
LOCUS       DADLTF010000340         1290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.346, whole genome shotgun sequence.
ACCESSION   DADLTF010000340 DADLTF010000000
VERSION     DADLTF010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.346"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN749_004339"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="JN749_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HAY7370476.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          387 a          209 c          307 g          387 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat
//
LOCUS       DADLTF010000341         1274 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.347, whole genome shotgun sequence.
ACCESSION   DADLTF010000341 DADLTF010000000
VERSION     DADLTF010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.347"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            370..1119
                     /locus_tag="JN749_004340"
     CDS             370..1119
                     /locus_tag="JN749_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAY7370477.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          360 a          258 c          256 g          400 t
ORIGIN      
        1 gctccttctg atgccattct atttcaggaa ggagtgtccg ttaaactcag gctacctcaa
       61 aaagttatcg gtgcgtttat cgaaaaacat atgttctact aattggagtc attgtctgta
      121 aaagcaactc aaaaatacaa gttgcttatt ccccttgttg gtaagaactt aattctaact
      181 gtttgttctg gatgttttaa acgtcctcct ttttatgctt gttcataacg gatatttaat
      241 aggtaacctt tgttttagca tcttctgatt tatttaaatt atgtgatagc gcacacaatt
      301 ttattatcca gtactttata ctcttaataa aataattata aatatttatc ctctataagg
      361 agatgttata tgaaaagaca gctttttact cttagtattg tcggggtatt ttctttaaat
      421 acctttgcat cattccctcc gggaaatgat gtgacaacaa aacctgacct ttactacctg
      481 acaaacgata atgctattga cagtctggca ttattaccgc ctccaccaca aatcggaagt
      541 attgccttcc tgaatgacca ggccatgtat gaaaaggggc gtttattgcg aaatactgaa
      601 agagggaagc tggcagctga ggatgccaat ctcagtagtg gtggcgtggc aaatgtattt
      661 tctgctgctt ttggttctcc gataacggct aaagattcac cagaattgca taaattactg
      721 acaaatatga ttgaggatgc cggagacctg gcaacgcgtt cagcaaaaga atattatatg
      781 cgtattcgac cttttgcttt ttatggtgtt tctacgtgta atacaaaaga acaggataca
      841 ttatccagaa atggctctta tccatcaggt catacatcga ttggttgggc aacagcactt
      901 gtattgtccg aaataaatcc ggcacgccag gataccattc tgaaacgggg ttatgaactg
      961 ggggacagca gggttatttg cggttatcac tggcaaagtg atgttgacgc agcacgtatt
     1021 gttggctctg ctattgtagc aacactgcac tcaaatcctg tgttccaggc acaattacaa
     1081 aaagcgaaag atgaattcgc taacaatcag aaaaaataat gcttttagcc agtacgcctt
     1141 ataaccccga ccttcagggc taatgccagt cagttaagca actgactggc tctttttcgg
     1201 ggctgtgggg tatttccagg gcctctcctt taccactctc gggaaggccc tttcccttct
     1261 tgtcggtaat ttca
//
LOCUS       DADLTF010000342         1272 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.348, whole genome shotgun sequence.
ACCESSION   DADLTF010000342 DADLTF010000000
VERSION     DADLTF010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.348"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..88
                     /locus_tag="JN749_004341"
     CDS             <1..88
                     /locus_tag="JN749_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370478.1"
                     /translation="LASNGSTLTIQSNGAAFTATTIKIIAEQ"
     gene            88..543
                     /locus_tag="JN749_004342"
     CDS             88..543
                     /locus_tag="JN749_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HAY7370479.1"
                     /translation="MIQFKPTRNIDLIEAVGNHTDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTFDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            546..1238
                     /locus_tag="JN749_004343"
     CDS             546..1238
                     /locus_tag="JN749_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HAY7370480.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSQQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
BASE COUNT          344 a          312 c          334 g          282 t
ORIGIN      
        1 tcttgcctca aatggcagta cgttaactat tcagtcaaat ggtgcggcgt ttactgccac
       61 aaccatcaag ataatcgcgg agcagtgatg attcagttca aaccaacgcg aaacatcgac
      121 ctgatcgaag cagtaggaaa tcacactgac attatcgccg ggagcaacaa cggcgatgga
      181 tacgactaca agcctgaatg ccgttacttt gaggttaacg tgcacgggca gttcggcgga
      241 attgtttact atcaggagat tcagccgcta acctttgatt gccacgccat gtacctgcca
      301 gagattcgtg gattcagcaa ggaaatcggg ctggcgttct ggcgatacat tctgactaac
      361 accaccgttc agtgcgtcac atcgttcgct gcgcgcaaat tccgccacgg tcagatgtac
      421 tgcgcaatga ttggccttaa gcgtgtagga accatcaaga aatacttcaa aggcgtggat
      481 gacgtgacgt tttacagcgc cacacgcgaa gaactaatcg acttcctgaa tcacgggaga
      541 tagccatgtt atatgcattt aagctgggca gaaaactgcg cggcgaggaa ccttattgcc
      601 ctgaaaaagg cgggaaaggt ggcagctctg ataaaagcgc aaagtatgca gcagaagctc
      661 agaagtatgc cgcagacctg caaaatcagc agtggcagac gatcatgaaa aaccttgctc
      721 cgttcacgcc tcttgcggag cagtatgtta accagcttca gaacctttcc agtttagaag
      781 gtcaggggca ggcacttaat cagtattaca actctcagca gtataaagac cttgcaggtc
      841 aggctcgtta ccagagtctt gctgctgcgg aggcgacggg tggacttggt tcgacagcca
      901 caagcaatca actggctacg atcgcgccga cactcggtca gtcttggtta tcaaaccaga
      961 tgagcaatta caacaatctg gcaaacgttg ggcttggtgc gctgcaaggt caggcaaacg
     1021 ccgggcagac atacgccaac aacatgagca gcattgcaca gcaaagcgca gctcttgccg
     1081 ctgctaatgc caacaaacca tcaagtcttc agacagcaat tagtggcgga acgtctggtg
     1141 cgattgccgg tgcaggtctt gccagccttt tgggaacatc aacaccttgg ggcgctggca
     1201 ttggtgctgg tatcggattg cttggctcgt tgttttaagg ggtaatcatg gctacttggc
     1261 aaggatcaaa tg
//
LOCUS       DADLTF010000343         1263 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.349, whole genome shotgun sequence.
ACCESSION   DADLTF010000343 DADLTF010000000
VERSION     DADLTF010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.349"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            66..1259
                     /locus_tag="JN749_004344"
     CDS             66..1259
                     /locus_tag="JN749_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HAY7370481.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
BASE COUNT          377 a          265 c          289 g          332 t
ORIGIN      
        1 aacgagacaa acatagcatt atgtgatcta ctgatttgtg ccaacaattc aatgagatca
       61 ctgctatgcc tgttcataaa gtatgtcaga atttcttccg ggatgcctta gcaccatttc
      121 atcagtaccg acaaaatgcc ctgatggatg ccacaatggc gttaatcaac ggagcatctc
      181 tgacgcagac cagtatcgga cgttttttgc cgggtaatgc acaagttaag aataagatca
      241 aacgtattga tcgcctcatg ggtaatgagg cgctccatcg cgatattcca atgattttca
      301 ggaatatcac ttcaatgctg acacggcagt tatctttatg tgtaatagcc gtagactgga
      361 gtggctatcc ctcccaggag catcatgttc ttcgtgccag ccttctgtgt gacgggcgtt
      421 ccattccgtt attgagtaag gttgttcctt ctgaaaaaca aaataatcca ttgatacaac
      481 atgatttcct tgattccctt gctcaatccc tgccccccga tgccagggtt attattgtca
      541 cggatgctgg atttcagagc gcatggtttc accatattac ttctctgggc tgggatttta
      601 ttggtcggat cagaaataac gttcagtatt gcctcgataa cgcgcctgaa agatggttaa
      661 aagtgtcgga tagccctgaa tgtaaaacac cggaatatat gggggctgga aggctggtta
      721 aagaaaggaa aaaaagcatc cgggggcatt tttacaccta taagaaatcg gcaaaagggc
      781 gaaaaaagaa acgttcgaaa ggtcagagtg gtttaaacaa aacagacaaa gaacagagca
      841 aatcagcaaa agaagcctgg ttgatcttca gcagtacaaa tgacttcaga gcgcgagaaa
      901 tcatcaagtt atacagtcgg cgaatgcaga tagagcaaaa ctttcgtgat gaaaagaacg
      961 ggcgcttcgg atttggtctt cgggccagca aaagtcgttc aacaggaaga attctggttc
     1021 tgagtctgct ggcaacgttg agcactatcg taatgtggtt actcggttat cacgctgaaa
     1081 ataaagggtt acacctgaaa tatcaggcta acagcattaa atcccggcgg gttatctcat
     1141 atctgacgtt agcgaagaat gttcttcgac actcgccact tattttaaga cgaacagtac
     1201 tgagcacagt tctaaaccat ctgtccagaa cctaccgaaa tatggtgctg gtttattagc
     1261 agt
//
LOCUS       DADLTF010000344         1255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.350, whole genome shotgun sequence.
ACCESSION   DADLTF010000344 DADLTF010000000
VERSION     DADLTF010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.350"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..203)
                     /locus_tag="JN749_004345"
     CDS             complement(<1..203)
                     /locus_tag="JN749_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HAY7370482.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYR"
     gene            complement(161..>1255)
                     /locus_tag="JN749_004346"
     CDS             complement(161..>1255)
                     /locus_tag="JN749_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HAY7370483.1"
                     /translation="VASPAQASVSDLTGDQPAAQPVPVSAPATSTAAVSAPANPSAEL
                     KIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEELLKSNTPLSVLALNQPENIENR
                     VNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSALGDRVANAFAQEWQKLGGGTV
                     LQQKFGSTSELRAGVNGGSGIALTGSPITPRATTDSGMTTNNPTLQTTPTDDQFTNNG
                     GRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFRLEMEGLQY
                     SEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQVQGFEINGN
                     TGSLTANPDCVINRKLSWLQYQQGQVVPAS"
BASE COUNT          246 a          359 c          343 g          307 t
ORIGIN      
        1 cgatagcgta cctcgacaaa aacggtggtc aggccttcac gcattatcag atcgatctcg
       61 ccgccacgct cgttcacgtt agcggcgata aaccgcagtc ctttgccttc cagccagcga
      121 cgcgcttgtg cttcccacgc atcgccggtc tgtttggtgg ttaactggcg gggactacct
      181 gaccttgttg gtactgtagc catgataact tcctgttaat cacgcaatcc gggttagccg
      241 tcagacttcc ggtattaccg ttgatttcaa aaccctgaac ctggcgcatt tgtgagaaat
      301 gatttgccaa cgaccaggca tcaacgccca tcgcatacat gcgagccagc gaataatcgt
      361 tgttcaccgc gctgagcgcc tgctgcatta acggcagatt accgcctgcc agcatcggga
      421 tttcgctgta ctgcaagcct tccatctcca gtcggaaatc cgggcctgcg gtgccttgtg
      481 cgctgcggga actggcgtac agcgttgcac cgctctggct accgttacgc atggcgatca
      541 tcggtttgat aaaagcgatt tcacccggcg ttgccacaat gtagaccgca tcgacacgac
      601 cgccattatt ggtgaactgg tcatcggttg gcgtggtttg cagcgttgga ttgttggtcg
      661 tcatgccgga gtcggttgtc gctctgggag taatcgggct acccgttaaa gcaataccag
      721 aaccgccgtt aacacccgcg cgtaattcgc tggtggaacc aaatttttgt tgcagaacgg
      781 tgccgccgcc cagtttctgc cactcttgcg caaacgcatt ggctacgcga tcgcccaatg
      841 cactgcgtgg gatcagcacc agcggcgctt gtttaccctg gtcacgaata tgacgcgctg
      901 catcgcgcgc ttcgtcttcc ggtgaaagcg cgaagtaaca aatattgacg cggttttcga
      961 tattctccgg ctggttcagt gccagtacgc tcagcggggt gttgctcttc agcaactctt
     1021 caacgttatt tttcagcaac ggaccaacca caatactcgc gccatcctgc tgaacctggc
     1081 ttaagatctg gctaagtggt tgtgatgagg tatcgtagat tttcagctct gcggatggat
     1141 ttgcgggtgc gcttaccgct gcggtgcttg tcgccggggc gcttacaggc accggctggg
     1201 ccgcaggctg atcaccggtc agatcgctaa ccgaggcttg tgccgggctg gcaac
//
LOCUS       DADLTF010000345         1244 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.351, whole genome shotgun sequence.
ACCESSION   DADLTF010000345 DADLTF010000000
VERSION     DADLTF010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.351"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1163
                     /locus_tag="JN749_004347"
     CDS             <1..1163
                     /locus_tag="JN749_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7370484.1"
                     /translation="LTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIE
                     EIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLT
                     ETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPN
                     ATEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFV
                     EGISDPNDSKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPD
                     CGAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDEL
                     NWKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            1141..>1244
                     /locus_tag="JN749_004348"
     CDS             1141..>1244
                     /locus_tag="JN749_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7370485.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDL"
BASE COUNT          387 a          300 c          313 g          244 t
ORIGIN      
        1 acctcaccga tcgcggcgaa ggtttcgtcc acgatctgac gtcactgacg cgcgatgtag
       61 ccactggcgt actggcccgt tcaatggacg tggacatcta taaccttcat ccggcacacg
      121 ctaaacgcat tgaggaaatt atcgctgaaa ataaaccacc cttttctgtt ttccgcgaca
      181 aattcatcac catgcctggc gggctggatt attcccgcgc catcgtggtt gcgtccgtga
      241 aagaagcacc aattgggatc gaggtcatcc ccgcacacgt cactgaatat ctgaacaaag
      301 tactgactga aaccgatcat gccaaccctg atccggaaat cgtggatatt gcctgcggtc
      361 gctcctctgc cccgatgccg cagcgagtaa cagaagaagg aaaacaggac gatgaagaaa
      421 aaccacaacc atctggcgca atgacagatg aacaggcaac ggctgaaaca gtggaaccga
      481 atgcaactga acatcatcag aacacgcagc cgctggatgc tcagtcacag gtaaattctg
      541 ttgatgagaa atatcaggaa ctgcgggcag aactccatga agcccggaaa aacattccgc
      601 ccaaaaatcc tgtcgatgca gacaaattac tggctgcctc tcgcggagaa tttgttgaag
      661 ggattagcga cccgaatgat tcgaaatgga ttcccggtca tcatatctcc tcaaatgaag
      721 tcaaaaaaac ggaaaatgaa gtgcgacaga ctgaagataa acagcaccag aacagcgaac
      781 cggaggaggg aacaccctgc aatcagactg gcgaggataa ctgccctgac tgtggcgcgg
      841 tgatgggaga cgcaacttat caggaaactt ttgacgaaaa aaaaccagat gaagcacagg
      901 aagaagagcc gaagaaaacg gaaaaagctg atgatttgtt accggagaac gctggcagcg
      961 atcagcataa tgatagcaat aatgaaactg gcgaagatga actcaactgg aaaaaacagg
     1021 tgttgattgc ggcggtttac ggtttatgtg cgaatcctgc gtgcatagcc tatgcgcctg
     1081 caatccctga catcgcaatg atgattgcca acaagcttga aaattttgga gtgaccagtg
     1141 atgagcgctt acccgatttt tgatcgtatt gaagaaatgg catgggctcg ccactaccag
     1201 cagatcgttc gtgaagaaaa agaaacggaa ctggcggacg acct
//
LOCUS       DADLTF010000346         1218 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.352, whole genome shotgun sequence.
ACCESSION   DADLTF010000346 DADLTF010000000
VERSION     DADLTF010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.352"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            761..1218
                     /locus_tag="JN749_004349"
                     /pseudo
     CDS             761..1218
                     /locus_tag="JN749_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015904490.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          302 a          287 c          371 g          258 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc tttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tgtcacagcc agtggcaggc
      841 tgacgctctg ataagtggac tgaggcagcg gcttgcgcaa tgcgggctgc agctgcatcc
      901 gcaaaaaaca cgaatagttt actgtaagga tgctgaccga cggggggact acccggaaac
      961 gagcttcaac tttctgggct atacattccg gccgcgtctg tcgatgaatc gctggggtaa
     1021 gaccttcgtt aacttcagtc cggcgatgag tgccagagca ggaaaagcaa tccgtcagga
     1081 ggtgaggaga atcgcagtga caagtccttg tacgagctgg cgcatatgtt caatgcgaaa
     1141 atcaaaggct ggataaagta ctacggtgct ttttataaat cagcactgta tctgacgctg
     1201 aggcaaatcg atcgcaaa
//
LOCUS       DADLTF010000347         1210 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.353, whole genome shotgun sequence.
ACCESSION   DADLTF010000347 DADLTF010000000
VERSION     DADLTF010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.353"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..332
                     /locus_tag="JN749_004350"
     CDS             <1..332
                     /locus_tag="JN749_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370486.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
     gene            332..679
                     /gene="tnpB"
                     /locus_tag="JN749_004351"
     CDS             332..679
                     /gene="tnpB"
                     /locus_tag="JN749_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7370487.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            699..>1210
                     /locus_tag="JN749_004352"
     CDS             699..>1210
                     /locus_tag="JN749_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7370488.1"
                     /translation="MDTSLSHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQILEAQERISALQEEMAETLGEQYDPILPS
                     PLRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCR"
BASE COUNT          289 a          337 c          329 g          255 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag cagataaata caccgtccga cagtctgtgc tgtgagctgg
      241 ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta ttacagatac
      301 ttatccgcga aataaaaggg agcagccact gatgatatct ttccctgcag gttcgcgtat
      361 ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg cctcaaaagt
      421 tcagaacgtc ctgaaggatg acccgttctc cgggcatctg ttcatcttcc gcggacgccg
      481 gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct ttaccagacg
      541 cctggagcgg ggccgcttcg tctggccggt cacccgtgac ggcaaggtgc accttactcc
      601 ggcccagtta tccatgcttc ttgagggcat cgactggaaa cacccgaaac gaacggaacg
      661 cgctggcatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca ctttctcatg
      721 agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc cagatggcga
      781 aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc aaccggctga
      841 aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa aaacttcgcg
      901 caaaaaccga acggcagata ctggaagcac aggagcgaat cagcgcactt caggaagaaa
      961 tggcggaaac gctgggtgag caatatgacc cgatactgcc atctcccctg cgccagtctt
     1021 cagcccgtaa accgttacct gcctcacttc cccgtgaaac ccgggtcatc cgtccggaag
     1081 aggaatgctg cccggcctgt ggtggcgaac tcagttctct gggatgtgat gtgtcagagc
     1141 agctggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg aaactggcct
     1201 gttgccggtg
//
LOCUS       DADLTF010000348         1207 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.354, whole genome shotgun sequence.
ACCESSION   DADLTF010000348 DADLTF010000000
VERSION     DADLTF010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.354"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..335)
                     /locus_tag="JN749_004353"
     CDS             complement(<1..335)
                     /locus_tag="JN749_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000986949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HAY7370489.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVD"
     gene            417..644
                     /gene="vapB"
                     /locus_tag="JN749_004354"
     CDS             417..644
                     /gene="vapB"
                     /locus_tag="JN749_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HAY7370490.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            644..1042
                     /gene="vapC"
                     /locus_tag="JN749_004355"
     CDS             644..1042
                     /gene="vapC"
                     /locus_tag="JN749_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HAY7370491.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(1051..>1207)
                     /locus_tag="JN749_004356"
     CDS             complement(1051..>1207)
                     /locus_tag="JN749_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="traD domain protein"
                     /protein_id="HAY7370492.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
BASE COUNT          303 a          336 c          298 g          270 t
ORIGIN      
        1 aaatccacgg cctggttatg tgcttcaata agacgtttgt cccccccgag catggccatc
       61 atggagatac ttttgggagc ggagaaggtc aggtcgtagc cgggacgatg cctgttactg
      121 ccatcctgca tgcggcttaa atccgccccg tccggcagcc tgccctccag aaggcgggta
      181 aagacatcct tatcgacact gccctgcagc cccagttgtt cagccccctg gccggcccag
      241 cgttctccca tgctgcccag cacatagtaa ttatccttgt cggtataata attaccggca
      301 cttccggccg atctgacctg cgcgatactc atcataaacg tatatccctt tgacatatcc
      361 cggtatcaat cccacaatag atatacacaa gacatatcca cataaggagg caaataatgg
      421 aaaccaccgt atttctcagc aaccgcagcc aggcggtcag actgccaaaa gcggttgcat
      481 tgccggaaaa cgtaaaacgc gttgaagtga ttgctgtcgg acgaaccaga atcattacgc
      541 cagccggaga gacatgggac gaatggttcg acggacacag cgtcagcacc gattttatgg
      601 ataacaggga acagcccggc atgcaggaaa gggagtcatt ctgatgctga agtttatgct
      661 cgataccaac atctgcattt ttacgataaa gaacaaaccc gccagcgtca gggagcgttt
      721 taacctgaac caggggaaaa tgtgtatcag ttcggtcacc ctgatggagc tgatatatgg
      781 tgcagaaaaa agccagatgc ctgaacgtaa tctcgctgtg atcgagggat ttgtttcccg
      841 cattgatgtt ctggattacg acgctgctgc agccacacac accggccaga taagagcaga
      901 acttgcccgt cagggacgcc ctgtcgggcc atttgatcaa atgatcgcag gtcatgcccg
      961 cagtcggggg ctgattattg tgactaataa cacccgggaa tttgaacgtg tgggcggcct
     1021 gagaactgaa gactggagct gacctgttca tcagaaatca tctcccggct caacatcctc
     1081 cccgcgttcc cggtgcacat tgatgttcac ctcttcacga cgctgcatct gctgccaggt
     1141 gtccggatga ttttcctgct gccatgcctc ataagcggcc atatccacca cttcaccgga
     1201 ttcactg
//
LOCUS       DADLTF010000349         1205 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.355, whole genome shotgun sequence.
ACCESSION   DADLTF010000349 DADLTF010000000
VERSION     DADLTF010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.355"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            14..925
                     /locus_tag="JN749_004357"
     CDS             14..925
                     /locus_tag="JN749_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7370493.1"
                     /translation="MINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGMHDKLQK
                     WYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTFAAREYC
                     RRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGGKETSGR
                     VNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDELARLFSR
                     FAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNHAWLAAK
                     GAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            941..1168
                     /locus_tag="JN749_004358"
     CDS             941..1168
                     /locus_tag="JN749_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370494.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          338 a          256 c          336 g          275 t
ORIGIN      
        1 cactattaac gctttgatta acgagggcga caagtctcag gaagatattg ctgatgaagc
       61 cggaatgtct ccggcaacac tctcggctct gcgtaaagga gagtacaacg gtaatgtcga
      121 aggtatgcat gacaaattac agaaatggta tcaggtctgg cagaagggac agaacttgtc
      181 aggtacccct ggtgttgtga agacccagat attttgtgat ttgcatgagc tgtttaaaac
      241 agtgcgctca ctcgggatta tcagtgtcgt tgttggcgta ccgggtgtcg gaaaaacctt
      301 tgctgcccgg gaatattgtc gcaggacagc taatgcctgg atggtgactc ttgctccggc
      361 acattcgagc gtaacggaat gcctccttga aatagcatat gcgctggaaa ttaataatcc
      421 gggaagaaat aaggggaata taacccgcgc cattcgcaga aaactgggtg gtggtaaaga
      481 aaccagtggc cgtgttaacc cccttctgat tattgatgaa gctgatcact taagtgtgga
      541 tggtctggag caattacgcg ccattcagga tgccactggc gtggggatgg ttttaattgg
      601 caacccgaaa cagatggcgg atgccacccg tcgtgggact gatgaactgg cccgtctgtt
      661 cagccgtttt gccatgacaa aacaactgcg caaagtaaaa aagaccgatg tgatggctgt
      721 agccaaagca tggggagtaa cacaggaaga tgagctggac ctgctgatga gggtagccga
      781 aaagcccggg ggattgcgtg tattaactca cacactgaat catgcgtggc tggcggcaaa
      841 aggtgcaggc acatccctga ataaaagtca cattaaagcg gcttttaagg aggcttacag
      901 caaccccagg ttactgaact ggtagtaaag gacataaacc atgatgtcac gaaatataaa
      961 aatggcaacg gatgcgcaga actggttaca ggcgcacggg agtcacgtaa atgaatcata
     1021 tctcggcgtg gcgcgtccgg tacttgaaat cacctgccca ccaccggagc tggtcagaaa
     1081 cgctgtcagg attatggagc ataaatcagg ggttgcccgt tcagtatgga cggcccgcct
     1141 taatggctgc cagattatct ggcgttaata acggcttatt tatttgtgag gaataacaaa
     1201 atggc
//
LOCUS       DADLTF010000350         1198 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.356, whole genome shotgun sequence.
ACCESSION   DADLTF010000350 DADLTF010000000
VERSION     DADLTF010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.356"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            88..606
                     /locus_tag="JN749_004359"
                     /pseudo
     CDS             88..606
                     /locus_tag="JN749_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            973..1198
                     /locus_tag="JN749_004360"
                     /pseudo
     CDS             973..1198
                     /locus_tag="JN749_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          372 a          264 c          246 g          316 t
ORIGIN      
        1 tgccggttac gccgaatctg ctgaatcaga actttacgcc cacagcacct aatcaggtct
       61 gggtcgcaga ttcagttgtc caggccttcc gaaatcaacc gactgaaggc gctggtcgcg
      121 aaactgcagc gtatgcagtt cggtaaaagc tcagaaaaac ttcgcgcaaa aaccgaacgg
      181 cggatacagg aagcacagga gcgaatcagc gcacttcagg aggaaatggc ggaaacactg
      241 ggtgagcaat atgacccggt actgccatct tccctgcgcc agtcttcagc ccgtaaaccg
      301 ttaccagcct cacttccccg tgccccccgg gtcatccggc cggaagagga atgctgtccg
      361 gcctgtggtg gtgaactcag tccactggga tgtgatgtgt cagagcagct ggagcttatc
      421 agcagcgcct ttaaggttat cgaaaaacaa cgccctaaac tggcctgtcg ccggtgcgac
      481 catatcgtgc aggcaccggt gccttcaaaa cccattgcac gcagttatgc cggagcgggg
      541 cttctggccc atgttgttac cggaaagtat gcagaccatc tgccattata ccgccaaagt
      601 gatctactat tccacacagc tatttagctc tctttttgtt gagctcccat gattctttca
      661 tttattgtta ctaattattt tatgttatca tgatgttaga acacgctctg ctctgtgtct
      721 ctattttatc tatcatattc tgaggattaa ctacataaca aggcgcaaga tatatatcca
      781 aaaacaaata ataccgttgc aataattcct ttcagtaaag gaggtgtgat taatttcagg
      841 ggatttcaga gtaaaaattg tacaacatat taaaatatgt tcatttcgtt ataatacaca
      901 caagaaaata ttaagttaga taatatattc taaataacca tagataaaaa cgcacataat
      961 ggggaatatt ttatgaaaat acctgaagca gtaaatcata ttaatgttca gaataatatt
     1021 gatcttgttg atggaaaaac gaatccgaat aaagcaacaa aagcattaca aaaaatatat
     1081 tacgcgtaac aaactcatct tcttctggca taagtgaaaa acatttagat cactgtgcta
     1141 atactgtgaa aaacttttta aggaagtcta tagcagcgca aagttatagc aaaatgtt
//
LOCUS       DADLTF010000351         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.357, whole genome shotgun sequence.
ACCESSION   DADLTF010000351 DADLTF010000000
VERSION     DADLTF010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.357"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            2..385
                     /locus_tag="JN749_004361"
     CDS             2..385
                     /locus_tag="JN749_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7370495.1"
                     /translation="MLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAFI
                     AVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLAL
                     NVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(386..1030)
                     /locus_tag="JN749_004362"
     CDS             complement(386..1030)
                     /locus_tag="JN749_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAY7370496.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          296 a          262 c          277 g          320 t
ORIGIN      
        1 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
       61 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
      121 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
      181 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctgtgc
      241 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
      301 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgatat tcccgctgct
      361 ggcacgtaaa gcgaatagca aataattatt gtggtcggtg acgctgactc tggcagatct
      421 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
      481 ggtaatagac ttggaagcct ggacgctctg tgatccagtc ttccagtaca cattctaaac
      541 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
      601 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
      661 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
      721 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
      781 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
      841 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgataa
      901 gagaatcgtc agttgtgagc tctactttaa tatcaggata ttcacgtgtg aaaccaacca
      961 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
     1021 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
     1081 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
     1141 gattgaataa gcgaa
//
LOCUS       DADLTF010000352         1155 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.358, whole genome shotgun sequence.
ACCESSION   DADLTF010000352 DADLTF010000000
VERSION     DADLTF010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.358"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(569..748)
                     /locus_tag="JN749_004363"
                     /pseudo
     CDS             complement(569..748)
                     /locus_tag="JN749_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            842..1155
                     /locus_tag="JN749_004364"
                     /pseudo
     CDS             842..1155
                     /locus_tag="JN749_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          318 a          212 c          298 g          327 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc ccctgaatct ccagacaacc
      781 aatatcactt atttaagtga tagtcttaat actagttttt agactagtca ttggagagca
      841 gatgattgat gtcttaggac cggagaaacg cagacggcgt actacacagg aaaagatcgc
      901 tattgttcag cagagttttg aaccgggaat gacggtctcc cttgttgccc ggcaacacgg
      961 tgtggcagcc agccagttat ttctctggcg caagcaatac taggagggaa gtcttactgc
     1021 tgtggccgca ggagaacagg tcgttcctgc ctctgaactt gctgctgcca tgaagcagat
     1081 taaagaactc cagcgcctgc tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc
     1141 cgttgaatat gggcg
//
LOCUS       DADLTF010000353         1144 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.359, whole genome shotgun sequence.
ACCESSION   DADLTF010000353 DADLTF010000000
VERSION     DADLTF010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.359"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            114..>1144
                     /gene="curA"
                     /locus_tag="JN749_004365"
     CDS             114..>1144
                     /gene="curA"
                     /locus_tag="JN749_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HAY7370497.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWGLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYLADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAG"
BASE COUNT          271 a          247 c          353 g          273 t
ORIGIN      
        1 ttcctcgatg aaattattgc gtgttgcaga ggggatgtga cggctgcaaa caaaaccaat
       61 cccgtgagcc tgctactatt gatggattca tgccaaaagc acaggagagc attatggggc
      121 aacaaaagca gcgtaatcga cgttgggttc tggcctcgcg tccacatggc gcaccggttc
      181 cggagaattt ccgtcttgaa gaagatgatg tcgccacacc gggtgaagga caggtgttac
      241 tgcgcacagt ttatttgtcc ctggacccgt atatgcgtgg acgtatgagc gatgagccat
      301 cttattcacc gccggttgat attggcggcg tgatggtcgg cggtacggtg agccgtgtcg
      361 tggagtcgaa tcatcctgat tatcagcctg gcgactgggg gctgggctac agtggatggc
      421 aggactatga catatccagt ggtgatgacc tggtgaaact tggcgatcat ccgcaaaatc
      481 catcgtggtc gctgggtgtg ctggggatgc caggctttac cgcttatatg gggctgctgg
      541 atatcggtca gcctaaagag ggcgaaacgt tggtggtagc tgcggcgaca ggaccagtgg
      601 gggcgacggt ggggcaaatc ggcaaactta aaggttgcag agtgatgggg gtagccggtg
      661 gcgcggaaaa atgccgccat gctaccgagg tgttaggctt cgatgtttgc cttgattacc
      721 tcgcggacga ttttgccgaa caactggcga aagcgtgccc aaaaggtatt gatatctatt
      781 atgaaaacgt gggcggtaag gtatttgatg cagtgctacc attacttaat acatctgcgc
      841 gcattcccgt ttgtggctta gtgagcagct ataacgctac agagctacca cccggtccgg
      901 atcgtttacc cctgttgatg gcaacggtgc tgaaaaaacg tattcgcttg caagggttta
      961 ttatcgctca ggattatggt caccgcatcc atgagtttca gaaggagatg gggcaatggg
     1021 tgaaagagga taaaatccac taccgcgaag atattactga tggtttagag aatgcgccac
     1081 agacgtttat cggcctgctg aagggtaaaa acttcggcaa agtggtgatc cgcgtcgcgg
     1141 gtga
//
LOCUS       DADLTF010000354         1141 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.360, whole genome shotgun sequence.
ACCESSION   DADLTF010000354 DADLTF010000000
VERSION     DADLTF010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.360"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..645
                     /locus_tag="JN749_004366"
     CDS             <1..645
                     /locus_tag="JN749_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vimentin yjdA"
                     /protein_id="HAY7370498.1"
                     /translation="EAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINTRLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRRRQRGLWGKICGAFGTSDWGW
                     ETYKEDVSRSVININTVRKEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCAFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTRRLQALNERVPELITDSKALREELETML"
     gene            642..>1141
                     /locus_tag="JN749_004367"
     CDS             642..>1141
                     /locus_tag="JN749_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY7370499.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGR"
BASE COUNT          299 a          275 c          322 g          245 t
ORIGIN      
        1 gaggctgaag cacagttccg gcctgagctg gagaaaatcg tcagcgggat tgaaacaggt
       61 tttcgtggca cggccctgta cgccacagaa aacattgccg gtcgcatcaa tacccgcctg
      121 gaggatgagg gctttaccgt aaaaatcagt tttccggcag tcagtcagtt acaaacccgg
      181 ctcgcggtaa aaacaaatct gagtgcgctt atggaggaaa gaacggagac cgtcacccgt
      241 cgccgtcggc agcgtggttt atggggaaag atttgtggag cgtttggcac cagtgactgg
      301 ggctgggaaa cctataaaga ggatgtgagc cgcagtgtta tcaatatcaa caccgtcagg
      361 aaggaagtta tgtcactgac ccgggcatat ttcggggagc tgcaggcatc cattgagcag
      421 gatattaacc agcccgtccg ccaggaaatc gatgcctttt tctgcgcatt cagggagaaa
      481 gttgaacaac tgcgtaacac gctgattcag agctctgaag atcataaacg cgatcagcag
      541 gcacaggaac ggcttaccag gcgacttcag gcattaaatg aaagggttcc tgagctcatt
      601 actgacagta aggcgctgag ggaagagctg gagacaatgc tgtgacttca ccatttattc
      661 aacaaattgc tgataaccgg gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg
      721 ttgatttcgc aaacggaatc gatgttgcac aggagcatat ccgcacggcc ggagagcgca
      781 cgttcttccg gcgtttaaaa gagggactga ccggcgaagg cgctgcacgg cagaatgcca
      841 tcaatgcttc ggttgcacag ggcgttgagg cgtccctgcg ctggctgacg gagctgacaa
      901 cgtcacttgc caccacaaac tacgcgatta cccgggtaaa cgacagggtc agttcactgg
      961 tcagtgatac tgccaggttg gcgcattatt ccgcagatac acgggagcag ttactcatcc
     1021 tggccgatca ggttcaccat aaactgaatc atcttgaaga aaaactccac cgtgttgacc
     1081 aggttcagcg ggcgcagtta catcttgagc agatattctc atggtggagc gccgggcgat
     1141 a
//
LOCUS       DADLTF010000355         1141 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.361, whole genome shotgun sequence.
ACCESSION   DADLTF010000355 DADLTF010000000
VERSION     DADLTF010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.361"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..645
                     /locus_tag="JN749_004368"
     CDS             <1..645
                     /locus_tag="JN749_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7370500.1"
                     /translation="EAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKTNLSALMEERTETVTRRCRQDGVWGTLCRWANTSDWGW
                     KEYSVDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCTFREK
                     VEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML"
     gene            642..>1141
                     /locus_tag="JN749_004369"
     CDS             642..>1141
                     /locus_tag="JN749_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY7370501.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLSGEGTARQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADQVHQKLNHLEEKLHRVDLVQRAQLHLEQIFS
                     WWSAGR"
BASE COUNT          298 a          268 c          327 g          248 t
ORIGIN      
        1 gaggctgaag cacagttccg tcctgaactg gaaaaaatcg tcaggggtat tgaaacaggt
       61 tttcgtggta cggccctgta cgccacagaa aatattgccg gacgcatcaa tgcccgtctg
      121 gaggatgaag gtttcactgt aaaaatcagt tttccggcag tcagccagtt acagacccgg
      181 ctcgcagtaa aaacaaatct gagcgcgctt atggaggaaa gaacggagac agtaacccgt
      241 cgctgtcgac aggatggtgt ctggggtacg ctttgtcgat gggcaaacac cagtgactgg
      301 ggctggaaag agtacagtgt ggatgtgagc cgcagcgtga tcaatatcaa caaggtcaga
      361 gaggaagtga tgtcactgac ccgggcatat ttcggggagc tgcaggcatc cattgagcag
      421 gatattaacc agcccgtccg ccaggaaatc gatgcctttt tctgtacatt cagggagaaa
      481 gttgaacagc tgcgtaacac gctgattcag agctctgaag atcataaacg cgatcagcag
      541 gcgcaggaac ggctgaccgg gcgtcttcag gcgttaaatg aaagggttcc tgagctcatt
      601 actgacagta aggcgttaag ggaagaactg gagacaatgc tgtgacttca ccatttattc
      661 aacaaattgc tgataaccgg gtatgtcagg tactcacctg tcttcctgaa aaatttgtgg
      721 ttgatttcgc aaacggtatt gatgttgcac aggagcatat ccgtacggcc ggagagcgca
      781 cgttcttccg gcggttaaaa gaggggctga gcggcgaagg tactgcacgg cagaatgcca
      841 tcaatgcttc gcttgcacag ggcgttgagg cgtccctgcg ctggctgacg gagctgacaa
      901 cgtcacttgc caccacaaac tacgcgatta cccgggtaaa cgacagggtc agttcactgg
      961 tcagtgatac tgccaggctg gcgcattatt ccgcagatac gcgggagcag ttactcaccc
     1021 tggccgatca ggttcaccag aaactgaatc atcttgaaga aaaactccac cgtgttgacc
     1081 tggttcagcg ggcgcagtta catcttgagc agatattctc ctggtggagc gccgggcgat
     1141 a
//
LOCUS       DADLTF010000356         1121 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.362, whole genome shotgun sequence.
ACCESSION   DADLTF010000356 DADLTF010000000
VERSION     DADLTF010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.362"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>1121
                     /gene="pdeF"
                     /locus_tag="JN749_004370"
     CDS             <1..>1121
                     /gene="pdeF"
                     /locus_tag="JN749_004370"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HAY7370502.1"
                     /translation="TYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVFLFYLP
                     LALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFT
                     PRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGT
                     LINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLAL
                     GALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSY
                     QNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLP
                     NVRALNRALRDALWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT          245 a          254 c          251 g          371 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt gggggcttcc gctgttcctg ccttctttaa
       61 tcttgcccat tttcgcccac attaatactt tcgcgcatat ttcttccggt gaggtttttc
      121 tcttttatct gcctttggca ctgatgatca gcatgatgat gtttttcagc tgggcggcat
      181 tgccagggat caccttaggg atttttgtcc gcaaatatgc agagctgggt ttttacgaaa
      241 cgctctcatt aacggctaat tttattatca ttatcattct ctgttggggc ggttacaggg
      301 tctttactcc ccggcgtaac aacgtttcac atggtgatac ccgtttaatt tcccagcgta
      361 tattctggca gatagtgttt cctgcaacgc tgtttctgat acttttccag tttgctgcat
      421 ttgtaggatt actggcgagc agagaaaatc tggtcggtgt catgcctttt aacctcggga
      481 ctttaatcaa ttatcaggcc ttactggtgg gtaatctgat cggtgtcccg ctgtgctact
      541 tcatcattcg ggtagtgcga aatccatttt atttacgtag ctattattcg caattaaaac
      601 agcaggttga tgccaaagtc accaaaaaag agttcgcgct ctggctactg gcattaggtg
      661 ctttattgtt gctgttatgc atgccgttaa atgaaaaaag cacaattttt agcaccaatt
      721 ataccttgtc attattgctg cccctgatga tgtggggagc aatgcgctat ggttataagc
      781 tgatttcgct gctctgggcg gtcgtgttga tgatcagcat ccacagctat caaaattaca
      841 ttcccattta tcctggctat accacgcagc tgaccataac ctcctccagt tatctggtat
      901 tctcttttat tgtcaattat atggctgtac tggcaactcg tcagcgagcg gtagtcagac
      961 gcattcagcg gcttgcgtat gtggacccgg tggttcatct gccaaatgtt cgcgccctga
     1021 atcgcgcgtt gcgtgatgcc ctctggtctg cgctttgtta tttacgcatc cctggcatgg
     1081 aaatgctggt taagaactat ggcatcatgc tgcggattca a
//
LOCUS       DADLTF010000357         1107 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.363, whole genome shotgun sequence.
ACCESSION   DADLTF010000357 DADLTF010000000
VERSION     DADLTF010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.363"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..387)
                     /locus_tag="JN749_004371"
     CDS             complement(<1..387)
                     /locus_tag="JN749_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HAY7370503.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQVDLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="JN749_004372"
     CDS             816..1097
                     /locus_tag="JN749_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370504.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          308 a          249 c          251 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctac ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaag
//
LOCUS       DADLTF010000358         1101 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.364, whole genome shotgun sequence.
ACCESSION   DADLTF010000358 DADLTF010000000
VERSION     DADLTF010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.364"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            88..153
                     /locus_tag="JN749_004373"
                     /pseudo
     CDS             88..153
                     /locus_tag="JN749_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="JN749_004374"
     CDS             381..629
                     /locus_tag="JN749_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7370505.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(745..1014)
                     /locus_tag="JN749_004375"
                     /pseudo
     CDS             complement(745..1014)
                     /locus_tag="JN749_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          324 a          248 c          237 g          292 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtca aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcacgtcc tgcatacgtc
      721 gtgtctgcgg tttttctttt tcgcttacat tgtgtctggt tcttccggcc actcaatatc
      781 aggtgcagtt gatgtatcaa cacggttcag caacacccga tacttctttc aggcttccag
      841 caacgagttt tcttcctccg ttgcgatctc cagatctaca gcatcctgaa gtggcgcaat
      901 atgctcactg aatccctgga tgtagaactg tgtggtgacg gtcttccagc cattcggctc
      961 ctgctgtatc gaagcatacc aggctatttc aatatcgcta tgctgcggca gcatttaacc
     1021 ccttgtaatt catcgccata attgatttaa ttcacaaata aaactataat atggtgaaat
     1081 taatgaaaaa aaacacagat g
//
LOCUS       DADLTF010000359         1099 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.365, whole genome shotgun sequence.
ACCESSION   DADLTF010000359 DADLTF010000000
VERSION     DADLTF010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.365"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..122)
                     /locus_tag="JN749_004376"
     CDS             complement(<1..122)
                     /locus_tag="JN749_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370506.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHL"
     gene            complement(194..1078)
                     /locus_tag="JN749_004377"
     CDS             complement(194..1078)
                     /locus_tag="JN749_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HAY7370507.1"
                     /translation="MVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFIGRVGDD
                     PFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSADLFLETT
                     DLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLWQDEHLL
                     RLCLRQALQLADVVKISEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVC
                     YRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGAL
                     AVTAKGAMTALPCRQELE"
BASE COUNT          293 a          317 c          229 g          260 t
ORIGIN      
        1 agatgtcctt tcagccaaat agcgtataac gaccaccaca gcgaccagga aataaaaaag
       61 agaaatgagt aactggatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc
      121 ataattacct gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc
      181 cgtttacttc tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg
      241 ccgttactgc aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct
      301 catctgtaga taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc
      361 ccgcccccgt gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc
      421 gataacagac caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact
      481 cttttgccag ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt
      541 cttcttccga gatcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga
      601 gcaaatgctc gtcttgccat agatcttcac gaatattagg atcgaagctg acaaaacctc
      661 cggcatgccg gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca
      721 acgcaattga acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct
      781 ctaaaaaaag atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt
      841 tcagatcgac aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat
      901 cgactccctc agttagcagc gttctttgca ttaacgcccc aaaaggatca tcaccgaccc
      961 gacctataaa cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg
     1021 cgccgccagg acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccacat
     1081 cccctaaaac ccatacttt
//
LOCUS       DADLTF010000360         1097 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.366, whole genome shotgun sequence.
ACCESSION   DADLTF010000360 DADLTF010000000
VERSION     DADLTF010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.366"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            101..1097
                     /locus_tag="JN749_004378"
                     /pseudo
     CDS             101..1097
                     /locus_tag="JN749_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          297 a          282 c          294 g          224 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat aaaaatcaat gagataaccg gttgccagtt
      481 aaatgccgga accgttcgcc gctggttgcc gtctgcgggg attgtgtggc gaagggctgc
      541 gccaactctg cgtatccgtg acccgcataa agatgaaaag atggcagcaa tccataaagc
      601 actggacgaa tgcagcgcag agcatccggt cttttatgaa gatgaagtgg atatccatct
      661 tcatcccaaa atcggtgcgg actggcaact gcgcggacag caaaaacggg tggtcacgcc
      721 gggacagaat gaaaaatatt atctggccgg agcgctgcac agcgggacag gtaaagtcag
      781 ctatgtgggc ggcaacagca aaagttcggc gctgttacat cagcctgctg aagcggctta
      841 aagcgacata ccgtcgggcg aaaaccatca cgctgatcgt ggacaactac attatccaca
      901 aaagccggga aacacagagc tggctgaagg agaacccgaa gttcaggggc atttatcagc
      961 cggtttactc gccatgggtg aatcatgttg aacggctatg gcaggcactt cacgacacaa
     1021 taacgcgtaa tcatcagtgc cgctcaatgt ggcaactgtt gaaaaaagtt cgccatttta
     1081 tggaaaccgt cagccca
//
LOCUS       DADLTF010000361         1093 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.367, whole genome shotgun sequence.
ACCESSION   DADLTF010000361 DADLTF010000000
VERSION     DADLTF010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.367"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..67)
                     /locus_tag="JN749_004379"
     CDS             complement(<1..67)
                     /locus_tag="JN749_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein hokD"
                     /protein_id="HAY7370508.1"
                     /translation="MLNTCRLASYVPKGKEKQAMKQ"
     gene            complement(270..488)
                     /locus_tag="JN749_004380"
     CDS             complement(270..488)
                     /locus_tag="JN749_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370509.1"
                     /translation="MFKCYCTLIVGGIMKNALQFLFVAFWLFASCMPIIFTARYMEKI
                     DVLILMFGHINALFLGVFMAVMCIEYWR"
     gene            complement(662..997)
                     /locus_tag="JN749_004381"
     CDS             complement(662..997)
                     /locus_tag="JN749_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370510.1"
                     /translation="MARVEGELFKIARASLEAEPIAWRYRYVKKGVMDSQGELWVGDW
                     KYVPKKEDCNDRPNYEIQALFTAPPVPVTSEELVKAVHFYEQLKRENPPASGNQINGL
                     TMSVKRPAN"
BASE COUNT          286 a          276 c          191 g          340 t
ORIGIN      
        1 gctgcttcat agcctgcttc tccttgcctt tcggcacgta agaggctaac ctacatgtgt
       61 tcagcatgga ttgagcctca gattaatgtt aagcgtcttg caggacgcgt aatgttaact
      121 ggggcttttc tctatctgcc ttttggtgtt catgcctgag acagatagcc tcaagcaccc
      181 gcagtcattc tacttaacta agatttccct gcaaaccgtt tttgtccggc acagtaaata
      241 tccaactaaa ccaatagcgt tcgctgtatt taccgccagt attcaatgca catgaccgcc
      301 atgaacaccc ctaaaaaaag ggcatttata tgtccaaaca ttaatatcaa aacatcaatt
      361 ttttccatat accttgctgt gaagatgatg ggcatacatg atgcgaacaa ccagaacgca
      421 acaaacaaaa actgcaatgc gtttttcatt attcccccta caatcaatgt gcaataacat
      481 ttaaacacac ctcaatttgg ccggacatat aaatatctaa accagaaaaa atcacttaca
      541 tagcgttaca aactctttag tctaaatact catcgtaaaa cattccccat acttatcagt
      601 ccgttccgcg ccaggtagct tattgcctta tctggcaacc tataatcagg tttccgcttt
      661 ttcagttggc tggtcgttta accgacatag ttaacccatt aatctggttg ccggatgctg
      721 gtggattttc gcgttttagt tgttcataaa agtgcacagc tttaaccagt tcttctgatg
      781 taaccgggac tggtggggca gtgaataagg cctgaatttc atagttcggc ctgtcgttac
      841 aatcctcttt tttcggtaca tatttccagt caccaaccca caactccccc tgagagtcca
      901 taacaccttt tttcacgtag cgatatcgcc acgctatcgg ctctgcttcc agcgatgccc
      961 gtgcaatttt gaataactcg ccctctactc gcgccatccc tgaattgggg tggcatttcg
     1021 taatcgctat ttttaatttg gcttcttcga ttaattgttc ttttgttaat tcagtcattt
     1081 tcattaccgc cct
//
LOCUS       DADLTF010000362         1085 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.368, whole genome shotgun sequence.
ACCESSION   DADLTF010000362 DADLTF010000000
VERSION     DADLTF010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.368"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..476
                     /gene="ybcL"
                     /locus_tag="JN749_004382"
     CDS             <1..476
                     /gene="ybcL"
                     /locus_tag="JN749_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HAY7370511.1"
                     /translation="NEIKTGEQLTTSHVFSGFGCEGGNTSPSLTWSGAPEGTKSFAVT
                     VYDPDAPTGSGWWHWTVANIPATVTYLPTDAGRRDGTKLPTGAVQGRNDFGYAGFGGA
                     CPPKGDKPHHYQFKVWALKTDKIPVDSNSSGALVGYMLNANKIATAEITPVYEIK"
     gene            486..>1085
                     /locus_tag="JN749_004383"
     CDS             486..>1085
                     /locus_tag="JN749_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAY7370512.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
BASE COUNT          341 a          203 c          222 g          319 t
ORIGIN      
        1 gtaatgaaat aaaaacagga gagcaactta caacgtctca tgtattttct ggatttgggt
       61 gtgaaggtgg taatacatcg ccctcattaa cctggtctgg tgctcctgaa ggcaccaaaa
      121 gctttgccgt aactgtgtat gatccagatg ctcctacagg cagtggatgg tggcactgga
      181 ctgttgctaa tattccagca actgtaacat atttgcccac tgatgcagga agacgtgatg
      241 gtacaaaact tccgactggt gctgttcaag gccgaaatga ttttggttat gcaggatttg
      301 gtggagcatg tcctcccaaa ggagataaac cccatcatta ccagtttaaa gtatgggctc
      361 taaaaactga taagattcct gtggattcta attccagcgg agcgttagtt ggttatatgc
      421 ttaatgccaa taaaattgca accgctgaga taacaccagt ttatgagata aagtagggta
      481 agagtatgct ggcaaaagat aagtctaacc taaagattga agaaatacgg atgcataaac
      541 atcatgagat tcacagggtt aagcctctta tgccagcttt atgtcgtatc cgtcagggaa
      601 aaaaagttat caattgggag acgcatagtc taactgttga taataatcag ataatattat
      661 ttccctgtgg atatgaattt tatattgtga attatccaga agcaggtctt tatcttgcag
      721 aaatgcttta ttatcccatt gatctaattg aaaagtttca aaaattttat gcgataactg
      781 atcaaattcg taacacgaca ggtttctgct tacctcagaa ccccgagtta atatattgtt
      841 gggagcaact aaaaacatct atttcccgag gcttctcaac gcaaattcag gaacacttag
      901 caatgggcgt tctactttca ttaggagcac atcatgttaa ttgtttactt ttatcagata
      961 gtaaacaatc attaataagt cgttgttata acctaatgct atccgaacct agaacaaaat
     1021 ggacagcaaa caaggtagcg agatatctct acatttctgt ttccacattg catcgccgtc
     1081 tggca
//
LOCUS       DADLTF010000363         1084 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.369, whole genome shotgun sequence.
ACCESSION   DADLTF010000363 DADLTF010000000
VERSION     DADLTF010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.369"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..561
                     /locus_tag="JN749_004384"
     CDS             <1..561
                     /locus_tag="JN749_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HAY7370513.1"
                     /translation="ADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVP
                     GITRAWAYRHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFR
                     PVAHTVDFHIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYS
                     HQLLAPADNISIAKNELAVLGTISWT"
     gene            552..>1084
                     /locus_tag="JN749_004385"
     CDS             552..>1084
                     /locus_tag="JN749_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HAY7370514.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSARDPAIAGAAPSLTRVHQRAD
                     VLMRELDPRTTTELINRWERLCGLPDECIPAGIQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPVATNTTWMTCGD
                     PCDSALRIWGDTVVECVL"
BASE COUNT          227 a          290 c          334 g          233 t
ORIGIN      
        1 gcagataccc tgacaggtgg atttgatact gaagatctgg aaacgtggcg cgcccgcgtc
       61 attgagcggt attactggac gccgcagggc ggggctgacg gggactatgt cgtctgggct
      121 aaagaagtgc ccggcattac ccgcgcatgg gcataccgtc actggatggg aacggggact
      181 gtcggtgtga tgattgccag tagtgacctg attaatccca ttccggaaga atcaacggaa
      241 acggcggcaa gacaacatat cgggccgctg gccccggtgg caggctctga tttgtatgtg
      301 ttcaggccgg tggcgcatac ggtggatttt catatccgtg tgacgccgga tacaccggaa
      361 atacgggctg ccatcaccgc ggagttgcgt tcgttcctgc tgcgtgatgg ttatccgcag
      421 ggagaactga aggtatcgcg tatcagtgag gcgatttccg gtgcgaacgg ggaatacagc
      481 catcagttgc ttgccccggc tgacaatatc tccattgcaa aaaatgaact ggcggttctg
      541 gggacgattt catggacgtg acaaacgatg attacatccg tctgttgtcg gcactgttgc
      601 cccccggtcc ggcgtggtca gccagagatc cggcgattgc cggtgcggca ccgtcattaa
      661 cccgcgttca tcagcgtgcg gatgtcctga tgcgggagct ggatccgcgc accaccactg
      721 aactgataaa ccgctgggag cgtctgtgcg gtctgccgga tgaatgtatt cccgcaggaa
      781 tacagaccct tcgccagcgt cagcaacgac tggatgcgaa ggttaacctg gcgggcggca
      841 tcaatgagga tttttacctt gcacagcttg ctgccctggg cagaccagac gccaccatca
      901 cgcgatacga caaaagcacg ttcacctgct catctgcctg tactgacgcg gtgaatgcgc
      961 cggaatggcg gtattactgg caggtcaaca tgccagtcgc caccaacacc acctggatga
     1021 catgtggcga tccctgtgat tccgcgctgc gtatctgggg tgacaccgtt gtcgagtgtg
     1081 tgct
//
LOCUS       DADLTF010000364         1070 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.370, whole genome shotgun sequence.
ACCESSION   DADLTF010000364 DADLTF010000000
VERSION     DADLTF010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.370"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>1070
                     /locus_tag="JN749_004386"
     CDS             <1..>1070
                     /locus_tag="JN749_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7370515.1"
                     /translation="AGTGRRVETHEYPNRDKPYTEDLGKVTFRPSITAYVVGDDCFDQ
                     RDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVSTSKGEGRIVRFDLKFVEAGELSY
                     PTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVADFVQNDVVGNASTMLGYVSDAMK
                     VVDSAVSDVARLLQGDISVLLPPPSSGKNFVEQVQKMWRTGKRLYGNASDLVTMIKTL
                     SGVSLGSDLQPRGVWKTDSKTTATATQQRNVVASTLRTTAISEAAYAVTRLPAPTTSA
                     VMQNAAVEQATTPAQSTGWPSVTHPALNNAPAVKNTVDLPTWEELTDIRDTLNTAIDK
                     ELSRTTSDALFLALRRVKADLN"
BASE COUNT          244 a          288 c          317 g          221 t
ORIGIN      
        1 tgcgggaacc ggtcgtcgtg tggaaacgca cgaatacccg aaccgcgaca aaccctatac
       61 cgaagatctg ggaaaagtca ctttccgccc gtccatcacg gcttatgtgg tgggagatga
      121 ctgctttgac cagcgcgatc gcctgattga cgcgctgaat aaacccggtc ccggcacgct
      181 tgtccatccg acatacggtg agctgaaagt ctgtgttgac ggggaggttc gggtcagcac
      241 atcgaagggt gaagggcgta ttgtccgctt tgacctgaag tttgtcgaag cgggagaact
      301 ctcttacccc acatcaggtg cggcgacggc gcagacgctg atgtcatcct gttctgcact
      361 ggatgactgc atcagtgaca gtttcagtgg tttcagtatc gatggcgtgg cagattttgt
      421 gcagaacgac gtcgtcggta atgccagcac aatgcttggg tatgtttctg atgcgatgaa
      481 agtggtggat tctgccgtat cggatgtcgc caggctgttg cagggggata tctcggtact
      541 tctgccgccg ccatcgtcag gcaaaaattt cgttgagcag gtgcagaaaa tgtggcgtac
      601 cgggaaacgc ctttatggta acgccagcga cctggtcacc atgatcaaaa cgctttccgg
      661 tgtcagcctc ggcagcgatc tgcaaccgcg cggcgtctgg aaaacggaca gtaaaaccac
      721 cgccacggcg acgcagcagc gtaacgtggt tgccagcacc cttcgtacga ccgcaatcag
      781 cgaagcggcg tatgccgtca cccgattgcc tgcgccaaca acttccgcgg tgatgcagaa
      841 tgccgcagtg gagcaggcaa caacacctgc gcagagcact ggctggcctt ccgtcacgca
      901 tccggcactg aacaatgcac cggcggtgaa aaacacggtt gacctgccga cgtgggaaga
      961 actgactgac attcgcgaca cactgaatac ggcaattgat aaggagttgt cccgtacaac
     1021 cagtgatgcg ctgtttctgg cgctgcgccg ggtgaaagca gatctgaatg
//
LOCUS       DADLTF010000365         1069 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.371, whole genome shotgun sequence.
ACCESSION   DADLTF010000365 DADLTF010000000
VERSION     DADLTF010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.371"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..428
                     /gene="ospC3"
                     /locus_tag="JN749_004387"
     CDS             <1..428
                     /gene="ospC3"
                     /locus_tag="JN749_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC3"
                     /protein_id="HAY7370516.1"
                     /translation="RDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIAL
                     YLLSKFNFTKQDVAEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKK
                     FQKANSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
     gene            766..1069
                     /locus_tag="JN749_004388"
                     /pseudo
     CDS             766..1069
                     /locus_tag="JN749_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611999.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          357 a          197 c          230 g          285 t
ORIGIN      
        1 tcagagacat atctttggag gatgcgatta aagcatctaa ttatgaagag attaacaata
       61 aagtgactga taaaaaaatg gctcatcagg cccttgctta ttctttaggc aataaaaaag
      121 ctgatatagc actttattta ctatctaaat ttaattttac aaaacaagat gtcgcagaga
      181 tggagaaaat gaacaacaat agatattgta atctgtatga tgttgaatac ttacttagta
      241 aagatggtgc caactataaa gtgctagaat attttatcaa taatggattg gttgatgtaa
      301 acaaaaaatt tcaaaaagca aatagtgggg acactatgct tgataatgca atgaaaagca
      361 aagattcaaa aatgattgat tttttattaa aaaatggagc aatattaggc aaacgatttg
      421 agatatgacc taaaagctcg gccaaataat aatgtcgtcg attatcgaag tcatcatgag
      481 tgattctgtc cccacagtgc agcatcagca tgacagtgaa ttgtctggcg tggtccattt
      541 aatgagatgg agatgctccc tctttcctaa atagctgtgc ctaataccgc tacacttttg
      601 ccagcccatg tttgcctccg gggaatgggc tgacggttcc ttcaaaatcc attgcacgca
      661 gttatgtcgg agcgggaact tgactcctgt ctttacggga gtgaggtagc ctgagtttaa
      721 cggacactcc ttcctgaaat agaatggcat cagaaggagc caataatgag cagaaaaaac
      781 caacgttact ctacggagtt taaagccgaa gccgtcaaaa cggttcctga aaatcagctt
      841 tcgatcagtg aaggcgcttc ccgattatct cttcctgaag gcactttagg acaatgggtt
      901 accgccgcaa gaaaagggct caatacacca ggctcccgca cagtggctga gctggaatct
      961 gaagttatgc aactgcgtaa ggctttaaat gaagcacgac ttgagcgaga tatattaaaa
     1021 aaagcaacag cgtattttgc ccaggagtcg ctgaaaaata cgcgttagt
//
LOCUS       DADLTF010000366         1043 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.372, whole genome shotgun sequence.
ACCESSION   DADLTF010000366 DADLTF010000000
VERSION     DADLTF010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.372"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..1013
                     /locus_tag="JN749_004389"
     CDS             <1..1013
                     /locus_tag="JN749_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7370517.1"
                     /translation="LLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGT
                     NCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKV
                     NDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIP
                     TDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPST
                     IMVALIVIHNCAVLGLFALENITMAEMFGSKNRFTRMAISKEIGGLIASGFGPILAGI
                     FCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSR
                     SL"
BASE COUNT          243 a          229 c          271 g          300 t
ORIGIN      
        1 tcttgctggt gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg
       61 ccggtacgat gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg
      121 tggctatggg aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct
      181 tcattctcag taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg
      241 ttgtcgtgat ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg
      301 agaaggttaa tgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc
      361 agagcaaatc cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt
      421 taattcagac tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc
      481 caacagatgc attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt
      541 ggttatccga taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg
      601 tgctggcgtg gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta
      661 tggttgcact gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca
      721 ttaccatggc agaaatgttc ggcagtaaaa accgctttac ccggatggct atttcaaaag
      781 aaattggtgg tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca
      841 tgacggaatc ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa
      901 tctctgcgct gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg
      961 cggaagatca accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca
     1021 atcccttctc ttaccggaga ggg
//
LOCUS       DADLTF010000367         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.373, whole genome shotgun sequence.
ACCESSION   DADLTF010000367 DADLTF010000000
VERSION     DADLTF010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.373"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..578)
                     /locus_tag="JN749_004390"
     CDS             complement(<1..578)
                     /locus_tag="JN749_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7370518.1"
                     /translation="MAHIQLVKQTSSGLLLPATSESCEFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYRTPVGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAA"
     gene            complement(645..905)
                     /locus_tag="JN749_004391"
     CDS             complement(645..905)
                     /locus_tag="JN749_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370519.1"
                     /translation="MKIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHLVDGLVTDGFLLCGDEFVTSLRALKETGLKAGFSAFECE"
BASE COUNT          259 a          278 c          223 g          265 t
ORIGIN      
        1 gcggccacat tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac
       61 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat
      121 gcgatagatt ttgccctgcg cccacggcta ccgtccggat aaatatgctc ggtgtaaaat
      181 ccggcctgaa tggttaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatcc
      241 cgggttctgc gtgtcgcaac ggtattcaga tattgctctg cggcttcgct cagagctggc
      301 gtatgttccc gacctactga ttcgcacagg tactcaacga agcctgatac cagttctcgt
      361 tctcgaggcg tgatcgcccc accgaccgga gtccggtaat cgaatcccag ttgcaggagt
      421 ttgaaaaaac gcttgtggaa tgcgtagtta cgaacgcgct taaaatcagc gtgtatccac
      481 tcgcctgttt tgatttgatg cagaaattcg caactctccg acgtcgccgg gagaagtaat
      541 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      601 gttactcagc agggggtcaa ccctgcactg aattgtagat gaactcactc acattcaaaa
      661 gcagaaaagc cagccttaag tccagtctct ttcagcgccc gtaatgacgt gacaaattca
      721 tctccacaca aaagaaaacc atccgtcaca agcccatcca ccaaatgaat taacgcagct
      781 ccactcttcc tttgttgaga ctgtaaacat ttaatacggc agtggctgac aatagcgcca
      841 ttctcaacgc gcacagtata gaggccatct tcactaaaaa tttcacgtaa ttcttcgatt
      901 ttcatcaaca gaatccttcc aggtaaatag cactcccctg ttcgggggcc atccctctta
      961 tccctgcgcg ctacttaagt gtttttgatt ctattcctgc gccttctgga agttcaaacg
     1021 agttg
//
LOCUS       DADLTF010000368         1025 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.374, whole genome shotgun sequence.
ACCESSION   DADLTF010000368 DADLTF010000000
VERSION     DADLTF010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.374"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..>1025)
                     /locus_tag="JN749_004392"
     CDS             complement(<1..>1025)
                     /locus_tag="JN749_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAY7370520.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFL"
BASE COUNT          303 a          269 c          219 g          234 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca tacaggagcg
      541 acataacaaa catcgcaatc cctgccacag aaccgacgat tttcagcgat ttcataccgc
      601 gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt aacgctacga
      661 ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc gcaatcagaa
      721 ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc cacgcggcga
      781 gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg cccttcccat
      841 ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa tagagtgcaa
      901 agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta ttaacaacgt
      961 tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat cgcaactgtt
     1021 tttcc
//
LOCUS       DADLTF010000369         1020 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.375, whole genome shotgun sequence.
ACCESSION   DADLTF010000369 DADLTF010000000
VERSION     DADLTF010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.375"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..769
                     /locus_tag="JN749_004393"
     CDS             <1..769
                     /locus_tag="JN749_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAY7370521.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            790..>1020
                     /locus_tag="JN749_004394"
     CDS             790..>1020
                     /locus_tag="JN749_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAY7370522.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQET"
BASE COUNT          332 a          226 c          231 g          231 t
ORIGIN      
        1 tcaatttgca acagataatg gttatacatc aggaaatcca tttaacggtc tgacaccatt
       61 aaaaaagtcc aagatagaac cagatcccct cacccgtgac gaattcattc gttttattga
      121 ggcttgccgt catcaacaaa caaaaaacct gtggattatc gctgtataca cgggtattcg
      181 tcacggggag ctggtatcac tggcctggga agatatagac cttaaagcga ggactataac
      241 catccgccgg aattatacaa aactaggcga attcactcca ccaaaaactg atgctggtac
      301 cggaagaaca attcatctag ttcaaccagc tattgatgct cttaaaagcc aagcggaaat
      361 gaccatgctt ggaaaacagc attctgtaga ggtaaagcag agggaatatg ggagaagtac
      421 tgtgcataaa tgcacttttg tttttagtcc tcaggtaata aaacagcggc agttttccgg
      481 accgcactat aaggttgact ccatcaggga gtcatggaca agtatcttaa aacgcgcagg
      541 tctgagacac agaaaatcgt atcaatccag gcatacctat gcatgctggt cacttgccgc
      601 aggagctaat cctagtttta tcgcaagcca gatgggccac acaaacgcac aaatggtatt
      661 caatgtttac ggagcatgga tgaaagacaa caatcacgaa cagatagaac ttcttaacag
      721 aagactatct gaaagtgtcc catgcatgcc ccataagaaa gcggggtaaa ataaaaactt
      781 gcaaaatcaa ttggtttacc tttaatccct gtcacgttac gcgcgtggca gaggcgttac
      841 ggattgctga aaccgcaacg gacagacggc ggtcatcggc tgttcaacga tgccgatatt
      901 gaccggatcc gcgagatcaa acgctggatc gacaacggcg tgcaggtcag caaagttaaa
      961 atactgctca gtaatgaaaa tgttgatgtg cagaacgtct ggcgcgatca gcaagaaaca
//
LOCUS       DADLTF010000370         1019 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.376, whole genome shotgun sequence.
ACCESSION   DADLTF010000370 DADLTF010000000
VERSION     DADLTF010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.376"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..273
                     /locus_tag="JN749_004395"
     CDS             <1..273
                     /locus_tag="JN749_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HAY7370523.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            275..>1019
                     /locus_tag="JN749_004396"
     CDS             275..>1019
                     /locus_tag="JN749_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370524.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVS"
BASE COUNT          323 a          186 c          202 g          308 t
ORIGIN      
        1 atccgtagaa atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg
       61 ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc
      121 ccttatagcc atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat
      181 atcaatgatt ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct
      241 tgtaatgttt ccgtagccaa acgagataaa taatatgcgc tacttattaa ttgtaattat
      301 atttattata ggttttagtg cattacctgt atgggcaatg gactgctatg ctgaacatga
      361 agagggaaat accgtagtca ttggttacgt accaagaatt tctatcccca gtaatggtaa
      421 aaaaggtgat aagatctggc aaagcagtga gtattttatg aatgtcttct gtaataatgc
      481 attacctgcc ccatcacctg gagaagaata cccatcagcc tggactaata taatgatgtt
      541 tttaccaggc ggtcaggact tttataatca gaactcctat atttttggcg taacatacaa
      601 tggagttgat tatgattcga ctgcccctaa tgctatcgca gcaccagaat gcattgatat
      661 aaaaggagcg ggtaccttta ataatcacta taaaaacccc gctgtctgta gtggaggtcc
      721 tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa ctctatatta aattagcaaa
      781 aaatgccaat agagttaata aaaacctggt gttacctgat gaatatatcg ctccagagtt
      841 taaaggtatg agtggcgcag gagctataga agtagacaaa aatttgacct tcaggattcg
      901 tggattaaat aatattcatg tccttgactg ctttgttaat gtcgcactgg aaccagctga
      961 tggcgttgtt gactttggta aaataaattc ccgaacaatt aaaaatacca ccgttagtg
//
LOCUS       DADLTF010000371          998 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.377, whole genome shotgun sequence.
ACCESSION   DADLTF010000371 DADLTF010000000
VERSION     DADLTF010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.377"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(36..326)
                     /locus_tag="JN749_004397"
     CDS             complement(36..326)
                     /locus_tag="JN749_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HAY7370525.1"
                     /translation="MNAHWSSKKSNFFRKNIKLLTKYLFFESQGIPDKVDIVSRLKTY
                     GYSISGVETDDGYKALVRAFQLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(295..546)
                     /locus_tag="JN749_004398"
     CDS             complement(295..546)
                     /locus_tag="JN749_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAY7370526.1"
                     /translation="MEPVYVILNALLDSGRFTRKLILLGLSGTFSYIFGSIVATLGMG
                     LVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLEFEKE"
BASE COUNT          278 a          262 c          219 g          239 t
ORIGIN      
        1 gaaggggata tatcgcagct ctgacgactc actccttatt tacccgggaa atacttctcc
       61 agcaatgcat acaggatggc agcggtttca gcatccatta tgccgtcata atttttctgc
      121 cggaaatgaa gctgaaaagc gcggacaagc gccttatatc cgtcatccgt ctccacacca
      181 gaaatactgt atccataggt cttcagccgt gacacgatat caaccttatc cggaatgccc
      241 tgcgattcaa aaaataaata tttagtcagt aattttatgt ttttcctgaa aaaattactc
      301 tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg caaaaacagc
      361 agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa caagtcccat
      421 tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca gccctaacag
      481 aataagttta cgcgtaaatc gcccggaatc cagaagagca ttaagtatca cataaacagg
      541 ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta gtccaccggc
      601 atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact ggtgtgagcg
      661 gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg cccgctaacg
      721 cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc gcgcacagag
      781 gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac tggtgatatt
      841 cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc tacatgtaac
      901 caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg tctgaaagta
      961 atcactatga ttaaatatga ttaacaacta atcgggta
//
LOCUS       DADLTF010000372          983 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.378, whole genome shotgun sequence.
ACCESSION   DADLTF010000372 DADLTF010000000
VERSION     DADLTF010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.378"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN749_004399"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="JN749_004399"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HAY7370527.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLHTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          219 c          221 g          284 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtatgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DADLTF010000373          979 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.379, whole genome shotgun sequence.
ACCESSION   DADLTF010000373 DADLTF010000000
VERSION     DADLTF010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..979
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.379"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            89..>979
                     /locus_tag="JN749_004400"
     CDS             89..>979
                     /locus_tag="JN749_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HAY7370528.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGGIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKAQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     EQIKPFLEKAMTMYNDVLFPG"
BASE COUNT          259 a          230 c          243 g          247 t
ORIGIN      
        1 tagttgttta ctgtgcgctg ttgtccatgt gatattgtgt accagatgtg tgccaaacta
       61 ttatttctga gcgtaccaaa tattatttat ggctataagc gacacaaaac tgcgtactat
      121 ttatggtaaa ccatattcgg gcccacaaga agtggctgat gccgatggcc tcagcgtacg
      181 aatttcaccg aaggggggca tccagttcca gtaccgctat cgctggcatg gcaagcctaa
      241 tcgacttggg cttggtcgat acccatccct gtctttgaag gatgccagac agatcactgc
      301 tgacttgcga aagctctatt tctcaggaac ggatccacgc acctattttg aagagaaggt
      361 ggagaactcc atgacggtcg cccagtgtct cgactactgg ttcgacaact acgtctctac
      421 aactctcaga gaaaaggccc aggcacttta tcgatcaacg gttatgaagc gcatgcatga
      481 cgccttccct aatcgtccgg catcttctat cacggttaag caatgggttg acctgcttac
      541 cgaagaagaa aaagataatc cacgccgagc aaggcaggtg ctaagtcaac taagatcagc
      601 aattagttgg tgcatgcggc gtcagttgat agatagttgc gcaattatga gcatccagcc
      661 aagggacttc ggctcccgcg ctgaggtagg ggatcgggta ctgtcgtatc acgaactggc
      721 taagatttgg cttgctattg aaagaagccg tgcgtctacg tcaaataagc tccttcatca
      781 gatgcttatg ctgtgggggg cgaggctctc agagcttagg ctggcaacaa agacagaatt
      841 tgacctgctg gacaacgtat ggaccgtacc gaaagagcat agcaagatgg gtaatgttat
      901 ccgccgtcca atcttcgaac aaattaagcc ttttctcgaa aaggccatga caatgtacaa
      961 tgatgttctt ttccctgga
//
LOCUS       DADLTF010000374          957 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.380, whole genome shotgun sequence.
ACCESSION   DADLTF010000374 DADLTF010000000
VERSION     DADLTF010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.380"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..98
                     /locus_tag="JN749_004401"
     CDS             <1..98
                     /locus_tag="JN749_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAY7370529.1"
                     /translation="YARLGFVQLSATPNAFGAVPMFLAIQHILAA"
     gene            complement(154..951)
                     /locus_tag="JN749_004402"
     CDS             complement(154..951)
                     /locus_tag="JN749_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HAY7370530.1"
                     /translation="MAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDR
                     EAPLALGSIYYDGEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKK
                     AKEYLDKGVHIYGGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCM
                     DSLFATTNKLGEVANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTS
                     NSSLILYKPEFERKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT          236 a          250 c          197 g          274 t
ORIGIN      
        1 tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt ggtgctgtac
       61 ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc acccgccagg
      121 gcaagttttg ccttagccgg gggctgagca gcgttaccac ttttgttcaa ttgcccccgc
      181 tttcagcaac tccacatcct tgccgttaat agttgccgtt gcggcaacta ttgtggtcct
      241 tacagttgca caagattttc gctcaaattc cggtttataa agaataagag acgaattact
      301 ggtaaatgaa ccaaataggt aatcagtaaa gctaatattc agccggttta aaccgaaagg
      361 cgcgaagcca accgtttggt taatctcctt accggatggg cgacgaatgc tgaatgtcac
      421 gcgaagattg gccacttcgc ctaatttatt ggtggtggcg aagaggctat ccatgcagga
      481 aaaaccttta tctaaaaaat tatcccgcat cccagaacgg gtaaccgtga tcacgggtaa
      541 cgtatcctca accgtttttc tgtcaatcat gtcttttcga caagtagcca gaaagtcttc
      601 cgggccacca taaatgtgga cacctttatc aaggtattcc ttcgctttct tacaatctac
      661 ctcaacatac tgaccattgg cgtacatcat gcccaacctg gaccatgcta aattcatacc
      721 ttgctgcgcg gcctgattaa atagtgcata cgctttggca taatccaccc gcacctgctc
      781 accgtcgtag tagatactcc ccagcgctaa cggagcttct ctgtcacctt gtttagcggc
      841 taactcgaac catttaatcg cttcctgaac atcaggtttg gtatcatagc gaggctgata
      901 atattcccag cctaacaggc tctgcgcgtc gatatctccc gattcagcca ttttatg
//
LOCUS       DADLTF010000375          923 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.381, whole genome shotgun sequence.
ACCESSION   DADLTF010000375 DADLTF010000000
VERSION     DADLTF010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.381"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..545)
                     /locus_tag="JN749_004403"
     CDS             complement(<1..545)
                     /locus_tag="JN749_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370531.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYA"
     gene            complement(545..910)
                     /locus_tag="JN749_004404"
     CDS             complement(545..910)
                     /locus_tag="JN749_004404"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370532.1"
                     /translation="MARSAKPRKRKPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDS
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVAPPF"
BASE COUNT          221 a          263 c          237 g          202 t
ORIGIN      
        1 gcgtaaaaat gaagattttt ccaggtgtgg cccgacgtca caccaccggc agttgtcgat
       61 aaatggatat gcggatgcca ctgctggtca cgcccccatg tgtggatcac cgtgaatatc
      121 cccggctcca catctgcctg atggcagatt tccagtatca catccgctgc aatgcggctc
      181 atctctgcca gtaaccaccg gttgtggaac accagggacc agtactggca gggaagtgtg
      241 aacacaatat gctgccacgg gcagtcgggg accaggctca gcagatactg tatccactgt
      301 gcgccagcct tcaccccgca gtgcgggcag gagcggcttt tacaccggaa gcagaccttt
      361 tttgtatggc aacagtccgg tgatgaacaa caccactgcg tgtagcccat cagtgtggtc
      421 ccgcaggcca tgatttttgt cactgattca attaccaccg gacggactgc cccttccggt
      481 tgtttctcca gccagttaag ccagcggttt ccctgctgaa aaatatcggc aaaacgggga
      541 agcatcagaa gggcggggcg actccgtccg gccagtgaac cgtgccacac tccgggcagt
      601 acataccgcc ggcgctgata ccggcaagaa tggtcgcaaa ttcccgctcc gtgcagcggg
      661 cgatttccgg atacccttcg tcatcaacac gtacaaacca gaagaccagc tttttgtttc
      721 ccgcatccac aaagaacgga atattcaggt ctgcgcagca ttcaacggca tcgtcaaaac
      781 tatcaaagcg cagaacttct gcgtcttctt cgtcaaaaaa atcatcttcg tgaagcttca
      841 cgacatagcg gggaagtttg cttctttgag aggcgggttt acgtttacgg ggtttagctg
      901 aacgggccat ataaccacct gaa
//
LOCUS       DADLTF010000376          911 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.382, whole genome shotgun sequence.
ACCESSION   DADLTF010000376 DADLTF010000000
VERSION     DADLTF010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.382"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            157..822
                     /locus_tag="JN749_004405"
     CDS             157..822
                     /locus_tag="JN749_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAY7370533.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          288 a          191 c          200 g          232 t
ORIGIN      
        1 aaagcttatt ttcagcttta attaactaac cacgtcattg ttctgctgct aataacaaca
       61 aaggtgggtg gtttatgatt attcacttta atacactagg tgaactcctt ctgccatgca
      121 ggcagggttg gacagaaaac acgcgaagga attaaaatgc ctggaacggg aaaaatgaaa
      181 catgtcagtt tgactctgca ggttgagaac gacctgaaac atcagcttag tattggcgca
      241 ttaaaacctg gcgcacgcct gattactaaa aatctggcgg agcaattagg tatgagtatt
      301 acacctgtgc gtgaagcatt attacgtctg gtttcggtga atgcgctttc tgtcgcacct
      361 gcacaagcat ttacagtacc ggaagtgagg caaagtcagt tagacgaaat caatcggatc
      421 cgctacgaac tggaattaat ggcagttgcc ctggctgttg aaaacctctc ctcgcaagac
      481 atagcagaac ttcaggaatt gctcgggaaa ttacagcagg cgcaagaaaa gggcgacatg
      541 gaacaaatca ttaatgcgaa caggctattt cgcttagcga tttatcatcg ctcaaatatg
      601 cccatcctgt gtgagatgat tgagcaactg tggatcagga tgggacctgc tttacattat
      661 ctttatgaag cgattaaccc agcggaatta cgggagcgca tagaaaacta tcatctatta
      721 ctcgctgcgt taaaagcaaa agacaaagag ggatgcaaac attgtcttgc tgaaattatg
      781 caacaaaata ttgctatttt ataccagcaa tacaatcgtt aaattaaatg cccggcaaat
      841 accgggcatc aagtaattac tcaaatcgcc acgcaatatt catgccgaca ccataatttc
      901 gtccgggcgc a
//
LOCUS       DADLTF010000377          901 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.383, whole genome shotgun sequence.
ACCESSION   DADLTF010000377 DADLTF010000000
VERSION     DADLTF010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.383"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..97)
                     /locus_tag="JN749_004406"
     CDS             complement(<1..97)
                     /locus_tag="JN749_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370534.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQ"
     gene            complement(84..455)
                     /locus_tag="JN749_004407"
     CDS             complement(84..455)
                     /locus_tag="JN749_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370535.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMISRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            complement(467..868)
                     /locus_tag="JN749_004408"
     CDS             complement(467..868)
                     /locus_tag="JN749_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HAY7370536.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
BASE COUNT          214 a          277 c          195 g          215 t
ORIGIN      
        1 tctgtgccac acggtttatc gcccatgcgc tggcctgtgg caccatacgg gtatcaaggc
       61 tgttcagatt gcggatggca ttctcaagcc ccttcataat atcgtcctgc ccttacgttt
      121 cccggcagcc cctcacgccg caaccggata taactacatc cgccatcatc aggggtgata
      181 cgatccacac agaaagactc acgactaatc atcagcgaat cattgcgaca cagccgtaaa
      241 atatctgctg ttttcacaaa cagggatggt aaagagtctt caatacgcac accgccggcc
      301 accagagaaa tattttcagg atcatcaaaa accccggaaa tcatgatccc ttttagccgg
      361 cctgacgtaa tgaccgctct aatccccata ttgcgaataa tggccttgtc tgcgagatca
      421 agtgcaacat caaacagatt atcgaaatca gccacgctgc ctcccgttat tgcattctgg
      481 ccaggccacg ttctgtcatt ccggctgcca caccggcaga gacacggaac gccattcccg
      541 gcggcacaaa tgccagaggt tcatcccgcg tggcatgaag tgcatcggta tgcagcgtca
      601 ccagtgccac gactgtaacc agatcatcca tattctgaat cacggtctcc ggctgcgctg
      661 atatcacctc attttcatgt ccggtcagcg cattttccgg gtgggcagac ttatcccgga
      721 tgtcagtgtc atccatattg gcaagttcct cctccagctc agccacacgc tgcgcaatat
      781 ctgctgcgct tcccgagata tccggctcac gtcccagcga tgctgccagt tctctcagac
      841 gtttcaggtt atcgtctttc tttgccatat ctgttcctgt ccgaataaaa aacgggagcc
      901 c
//
LOCUS       DADLTF010000378          897 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.384, whole genome shotgun sequence.
ACCESSION   DADLTF010000378 DADLTF010000000
VERSION     DADLTF010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.384"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..129
                     /locus_tag="JN749_004409"
     CDS             <1..129
                     /locus_tag="JN749_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7370537.1"
                     /translation="SSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            126..680
                     /locus_tag="JN749_004410"
     CDS             126..680
                     /locus_tag="JN749_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7370538.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
BASE COUNT          235 a          194 c          256 g          212 t
ORIGIN      
        1 agctcatgcc acgatttaat cgacgggcgg gtaaaaacca gcgattacac caaagaagaa
       61 ttacgcctga tgcatgcaga aggtgttttt cgcacacaag aaatctggag aaaggaaggt
      121 tatttatgat ttacccaaca aatacaggca aaagcgggga acaccttcgt ctcaccacgc
      181 tggaaagtgt ctggattcag ggaaaactgc gcatgtgggg gcgctggtcg tatattggcg
      241 gcggtaagac gggaaatatg tttaaccagt tgctggcatc caaaaaattg acgaaaacag
      301 ccgtcaatga agccctgcgc aggatgaaaa aagcgggaat agagaaacct gagctggaag
      361 cgtttttgcg agagatgatc aatggcaagc aaaagacctg gctggcgcat tgtactgatg
      421 cagaggcgtt atgtattgat agagtcataa gtgaggtgct ggcagagcat cctggattga
      481 ttagtgtcct ccggcaacgg tatgaagggc gggggatgac taagcgtaaa atggctgaat
      541 tgctgaatga cgcacaccct gagtggtgtt tccggacgtg ctgcagtcgg gtagatgtat
      601 ggctaaatct tgctgaatat atgctctatc taccgatgcg tgatgcattc tcttccgggg
      661 atctaaaaac cgtttgttga ctcaatctgt tatccggggc tatattcctc acgcgcagca
      721 aaatctggcg tcgggattgg cgtcccggat gtttacggag cgatatgaga cgcgcccgcg
      781 tcttttttca tatcgtttgc acagtcacat tcgcgattta tggcgggctg tgtgggggag
      841 ccgaaaggct cgccggtttc cgtacccggt tacgccaacc ctgcacagtt cgccacc
//
LOCUS       DADLTF010000379          895 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.385, whole genome shotgun sequence.
ACCESSION   DADLTF010000379 DADLTF010000000
VERSION     DADLTF010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..895
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.385"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(110..439)
                     /locus_tag="JN749_004411"
     CDS             complement(110..439)
                     /locus_tag="JN749_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HAY7370539.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..895)
                     /locus_tag="JN749_004412"
                     /pseudo
     CDS             complement(611..895)
                     /locus_tag="JN749_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          219 a          207 c          199 g          270 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 caatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttacgcgtt caataacacg aaatg
//
LOCUS       DADLTF010000380          893 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.386, whole genome shotgun sequence.
ACCESSION   DADLTF010000380 DADLTF010000000
VERSION     DADLTF010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.386"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            18..407
                     /locus_tag="JN749_004413"
     CDS             18..407
                     /locus_tag="JN749_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HAY7370540.1"
                     /translation="MKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGITS
                     FSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQLIG
                     IGVLGEYIGRIYIEVKNRPKYIIKDKK"
BASE COUNT          270 a          149 c          162 g          312 t
ORIGIN      
        1 ctgagcgcaa tcttttcatg aaaggcatac tgagctgggt gggtggtcag acagatgtcg
       61 ttgaatatgt acgcgcagag cgtgttgctg gcatctcaaa atttaatggc tggaaattat
      121 ggaatctggc actggaaggt atcacaagtt tttcaacctt tcctcttcgc gtatggactt
      181 atataggctt gtttgttgca agcatttcgt ttttatatgg tgcatggatg attatagaca
      241 cccttgtctt tggtaaccca gtacgcgggt atccctccct gcttgtatca atacttttct
      301 tgggtggcgt gcaactgatc gggattggtg ttctcggaga atatataggc agaatatata
      361 ttgaagtaaa aaatagacct aaatatatta ttaaggataa gaaatgatta aatcaaatta
      421 catatgcttt cttttgcttt ttttattggt ttaactattc ttcatttaaa tcaatataca
      481 ctagtatcag atgacatttg gttttccgag aaatcaatat cagaaccgga tcacatcaag
      541 tggctcatag atagatattt cacatggtca tcacgaacat agattgaata tgcattaata
      601 aatcttatta atcattttta tgcttggtct ttaattaatg cagcaatgtt ttcatcatta
      661 attattggca tttgctctat ctttcagaga aaaaacaacc tttactataa aataaacact
      721 tcaatcatgt cattattact gattgttatg atgccaagcg atgttttttt caatgccgcg
      781 atttggataa caggatcatt caactatctg tggccaagtg ctattgcatt tattggttat
      841 tctctactta taaaaaaaat caatatggaa aaccacgaga aaactcaatc tct
//
LOCUS       DADLTF010000381          888 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.387, whole genome shotgun sequence.
ACCESSION   DADLTF010000381 DADLTF010000000
VERSION     DADLTF010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.387"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(102..791)
                     /locus_tag="JN749_004414"
     CDS             complement(102..791)
                     /locus_tag="JN749_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HAY7370541.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTAHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
BASE COUNT          219 a          241 c          171 g          257 t
ORIGIN      
        1 atttttcagg cataaaaaaa ccgctcggcg gcgggttttg cttactttac catcgcgtac
       61 aaaatcggca aagtatcaga tttgcatgaa atatatgcct tttaatctac ttttgcaata
      121 ctttgctttg aaaatgccgc tttttgttct gaacgtgttc tcatcacaaa caataaagcc
      181 tcactatcca gtcggtgaaa aatgtgtttc attacaaccc agtgagcagt aaatgttttg
      241 gaccagtttt tggttgttac tcccgccagt aatgccagct cctgatattc ataaccttcc
      301 ccaccaaaaa gttctgcttt taccgcctgc gccgccagcc agattaattt tttcaggcgt
      361 tcctgtgttt tctctgcaat tttcctagtg ccagaatgag ccttaaactc ttcccacgcc
      421 cagtgggtta tggtgatctg atactcccag caaatacttc cgctgtaaca ccacaacaac
      481 caggctttat ggtgctcttc aagagacaga acagcccgcc gccacgatga tgtcgaaaac
      541 gcaacctgac tgaccagagg aattgacgcc cccttcgcca gtgattgttt tcccgggatt
      601 ggtggattat cccgcgttat catttttccg gtcacctcat cgcggtaccg gattttttta
      661 cgcctgtaac gccctgtatc gaacatggca ttctcctgcc aggcttcaag ctgacctttt
      721 gtcgccccac tcaaatcagc ggtggcgata atgagctgct cacgaacaaa ctgtaaatac
      781 tggttattca tgcacactcc agctctgtga tttttatccc caaccgacca ccaggaacag
      841 gcagtccgcg cacaatattg gtttcatcaa actgctcgtc gtcgataa
//
LOCUS       DADLTF010000382          867 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.388, whole genome shotgun sequence.
ACCESSION   DADLTF010000382 DADLTF010000000
VERSION     DADLTF010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.388"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..>867)
                     /locus_tag="JN749_004415"
     CDS             complement(<1..>867)
                     /locus_tag="JN749_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7370542.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVS"
BASE COUNT          227 a          223 c          197 g          220 t
ORIGIN      
        1 cgaaacagaa taggtggcgt aactattacc ccaccagccc gataaaggaa cagataaaga
       61 gagcatgtag atctgctcac tggtgttatc actggcatgg ttgttccccg tcccctgtct
      121 ggcagtattg gaataactcc aggagacgtt ccacgagaga cgcttccagc tactgctgta
      181 gccaaattgc attaatcgtt cgacatcatc ggtatgccag tattgttgcc ggcttaatgt
      241 caggtataaa ttgccataat ctctccccaa cgactgcgtt agcgttccct ccacgcgact
      301 acgtcggtta cctgtttccc aaaagtcttc agggctgttg cgccgcgatg cccactcatt
      361 gagggtatag tagccctcgg tggagtaacg atagccaacc aggcgcaagc tagtgtctgt
      421 gtcgtcaaat agttttgaat agagaaagcg atatgactga cccgtttctg tatcatcatg
      481 gctaaaattc gcacgagcat gtgtcacatc gaaagaaatt gcgcccaaag cgccaagatc
      541 tttaccgaca cccagagctg cagaaccata attttcagca gcctgcatac ctccatataa
      601 agtgatcccg tgggaaaaac catgaagtat ttgcgcctga aggacgtccg gtgtaaatcc
      661 gtcttcatcg cgcaattcac ccacgctgac atcaacatct gtcagacctt cacgtttaag
      721 aatcgccaat gaagcataag gttgggtgaa tcgttgttcg ctaccgtctt cttctttgat
      781 ggttacgtcc aaatcgccac ctgtacttgc ggtgttgaga tcggtgatct caaaagcacc
      841 ttgtggaacg gctgactgat aaatcac
//
LOCUS       DADLTF010000383          865 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.389, whole genome shotgun sequence.
ACCESSION   DADLTF010000383 DADLTF010000000
VERSION     DADLTF010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.389"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..347
                     /locus_tag="JN749_004416"
     CDS             <1..347
                     /locus_tag="JN749_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAY7370543.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
     gene            702..>865
                     /locus_tag="JN749_004417"
     CDS             702..>865
                     /locus_tag="JN749_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HAY7370544.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRT"
BASE COUNT          253 a          204 c          194 g          214 t
ORIGIN      
        1 cccccgctaa agtgttgccc gaaaactggt ggcaacatcc agctgcatta ggtgcaactg
       61 acagtgatat cgaaatcatc aaacgtcagt ggggtgcgtt ttacggtacg gatctggagt
      121 tgcaactgcg ccgccgaggt atcgatacaa tagtgttatg tgggatctcg accaatatcg
      181 gtgttgaatc caccgcccgc aatgcctggg aacttggctt taatctggtg attgccgaag
      241 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
      301 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      361 accgcaatgg tcgcatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      421 ccgccacttt aactgcgtga tacgggattt ttaaaaataa ctaaagaacg cccaagagca
      481 tgtgttttct ttagaataat caaagcgttg tattgccatg tcatatgaaa atcggcaaag
      541 ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc
      601 cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt
      661 aaaaataata ctgtataaaa acacagtgta gagggatttt tatgcgtatt gaaatctgca
      721 tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta aaggaagaat
      781 taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaaa gccaccagca
      841 acgatggcct ttctgttacg cgcac
//
LOCUS       DADLTF010000384          856 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.390, whole genome shotgun sequence.
ACCESSION   DADLTF010000384 DADLTF010000000
VERSION     DADLTF010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..856
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.390"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..282
                     /locus_tag="JN749_004418"
     CDS             <1..282
                     /locus_tag="JN749_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370545.1"
                     /translation="RQRAARTDETTYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTE
                     LLVSEVTFTQDQNGTLTEIRVGPPDAYLPEPEAPGARKKKKARVQEDPF"
     gene            282..841
                     /locus_tag="JN749_004419"
                     /pseudo
     CDS             282..841
                     /locus_tag="JN749_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
BASE COUNT          200 a          211 c          281 g          164 t
ORIGIN      
        1 cggcaacggg cggcgcggac ggatgaaacc acctatgtgg tgcagggctg gcgacagggt
       61 aacggtacgc tgtggcagcc caaccagcgg gtgattgtct ttgatccggt ctgtggtttc
      121 gacaataccg aactgcttgt ttcggaagtc acgtttactc aggaccagaa cggcaccctg
      181 acggaaatcc gtgtcggccc gcctgatgct tatctgcctg aacccgaagc ccccggcgcg
      241 cggaaaaaga aaaaagccag agtacaggag gacccgttct gatgaggacg attgaagcca
      301 tgcagcgaca actcctcggc ctgattgggc gggcagtggt gaaaagcatc agtgccgcca
      361 cgaaatgtca gaccgtggat gtgtccctga ttgccggtga acccaaagcc ggggttgaac
      421 atcttgaacc ctacggtttt accgcaaggg caaacagcgg tgcggaagcg gtgcggaagt
      481 ggtggtgttg tttccggatg gcgaccgttc tcatgcggtg gttgttacgg tgtcggaccg
      541 gcgctaccgc ctgaaagggc tgcagacggg tgaggtggct gtctatgacg atcaggggca
      601 gtctgtgacg ctgacccggg aggggatcgt ggtggacggt acaggtaaaa cgatcacgtt
      661 tcgcaatgcg cctgaagcac gttttgaaat ggacctggaa gtaacaggac aggtgaaaga
      721 cctgtgcgac tccggcggca ccaccatgtc agcaatgcgg cttgcctata acggtcatcg
      781 tcacagagag aacggtcagg gcagtaacac cgataaacct gataaagcta tggaggcatg
      841 atggaactgt ggctga
//
LOCUS       DADLTF010000385          853 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.391, whole genome shotgun sequence.
ACCESSION   DADLTF010000385 DADLTF010000000
VERSION     DADLTF010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.391"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..761
                     /locus_tag="JN749_004420"
                     /pseudo
     CDS             1..761
                     /locus_tag="JN749_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          231 a          185 c          202 g          235 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcgctgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggttgaaggt ctaaattttg ctctgcaata tcagggtaaa aatgaaggta
      421 caggcaacta caaagcaaat ggcgatggcc atggtttatc tgcaacttac actattgacg
      481 gtttctcatt tgctggtgct tatgcaaact ctgaccgtac tgattggcag tctggtgatg
      541 gtaaaggcga aagagccgaa gtttgggcgt tgtcaacaaa atatgacgct aacaatgttt
      601 atgctgctgt gatgtacggt gaatctcaca acatgaattc cgacgacggt gatgttgtga
      661 acaaaacaca aaacttcgaa gctgttctgc aatatcagtt cgactttggt ctgcgtccat
      721 ccatcggtta ctcctattct aaagcactgg acgtagcagg ttggatttgc ccctatattt
      781 ccagacacct gttatcactt aacccattac tggcccgctg ccgtagatat tcccgtggcg
      841 agcgataacc cag
//
LOCUS       DADLTF010000386          830 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.392, whole genome shotgun sequence.
ACCESSION   DADLTF010000386 DADLTF010000000
VERSION     DADLTF010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.392"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..326
                     /locus_tag="JN749_004421"
     CDS             <1..326
                     /locus_tag="JN749_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HAY7370546.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="JN749_004422"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="JN749_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HAY7370547.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          255 a          181 c          153 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca ggttctatgt tcttcatcca tgttaccagt aacggccaga
      481 atatcgacca agcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgatccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag aaaaatgtaa
      781 gaagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga
//
LOCUS       DADLTF010000387          828 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.393, whole genome shotgun sequence.
ACCESSION   DADLTF010000387 DADLTF010000000
VERSION     DADLTF010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.393"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..243
                     /locus_tag="JN749_004423"
     CDS             <1..243
                     /locus_tag="JN749_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HAY7370548.1"
                     /translation="RCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGD"
     gene            265..384
                     /locus_tag="JN749_004424"
     CDS             265..384
                     /locus_tag="JN749_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370549.1"
                     /translation="MIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            381..>828
                     /locus_tag="JN749_004425"
     CDS             381..>828
                     /locus_tag="JN749_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY7370550.1"
                     /translation="MSIEIKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDGPLTALMDHYLDTDALA"
BASE COUNT          191 a          192 c          252 g          193 t
ORIGIN      
        1 cgttgttatg tggcacttga agagcttcgc tggggcgcgt ttggtgatgt gatacgtcag
       61 ggcgaaacag gccaggttaa ccagctactg gatattctca gacataaagc attaacgcag
      121 atggcacagg agagtggcgg tagtgcaacc gtgcgtctga acactctgga ctggcttggt
      181 ggtcagggcc gggagcaggc agataacgag tggcatgacg cgattaactg gctgggagac
      241 tagtgcagtg aagagcagca tccggtgatc tggtcaacca cgcaggctgc agaacactta
      301 ccggttcgca tgccccgtct ctgctctgca gaacgcctct ctgaaagtat ggtcgatgaa
      361 atatttcaga agggggctgc atgagtatag aaataaaaac agggctggtg ctgtccggcg
      421 gtggggcggt gggcgcttat caggcgggag tggttaaggc actggcagag tgtggtacac
      481 agatcagcat ggtttcaggg gccagcattg gcgcattcaa tggtgccatt atcgcggcct
      541 ctcccgatct gtcagaagct gccgtacgcc tggaggcgct ctgggatcat cttgggaata
      601 atcaggtgct gtcggtaaac cgatcggttt acttttcatt gctgaaaaaa ttgttccagg
      661 caatgaacct ctgccagatc cccggacgtg caggagcact gcttacgaca cttcttcgcc
      721 atatatcgat aatcaacggc ttcgacaatc cgatgactca gccgttgttg tcagatgggc
      781 ccctgacagc gttgatggat cattatcttg atacagatgc tctggcag
//
LOCUS       DADLTF010000388          827 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.394, whole genome shotgun sequence.
ACCESSION   DADLTF010000388 DADLTF010000000
VERSION     DADLTF010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.394"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>827
                     /locus_tag="JN749_004426"
     CDS             <1..>827
                     /locus_tag="JN749_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7370551.1"
                     /translation="KSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCIS
                     DSFSGFSIDGVADFVQNDVIGNASIMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPP
                     PSSGKNFVEQVQKMWRTGKLLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTA
                     TATQQRNVVASTLRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTCWPSAT
                     HPALNNAPAVKNTVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLN"
BASE COUNT          189 a          223 c          245 g          170 t
ORIGIN      
        1 gaagagtgaa gggcgtattg tccgctttga cctgaagttt gtcgaagcgg gagaactctc
       61 ttaccccaca tcaggtgcgg cgacggcgca gacgctgatg tcatcctgtt ctgcactgga
      121 tgactgcatc agtgacagct tcagcggttt cagtatcgat ggtgtggcgg atttcgtgca
      181 gaacgacgtt atcggtaatg ccagcataat gctggggtat gtttctgatg cgatgaaagt
      241 ggtggattct gccgtatcgg atgccgccag gctgttgcag ggggatatct cggtacttct
      301 gccgccgcca tcgtcaggca aaaatttcgt tgagcaggtg cagaaaatgt ggcgtaccgg
      361 gaaactcctt tatggtaacg ccagcgacct ggtcaccatg atcaaaacgc tttccggtgt
      421 cagcctcggc agcgatctgc aaccgcgcgg cgtctggaaa acggacagta aaaccaccgc
      481 cacggcgacg cagcagcgta acgtggttgc cagcaccctt cgtacgaccg caatcagcga
      541 agcggcgtat gccgttaccc gattgcctgc gccaacaact tccgcggtga tgcagaatgc
      601 cgcagtgggg caggcaacaa cacccgcgca gagcacttgc tggccttccg ccacgcatcc
      661 ggcactgaac aatgcaccgg cggtgaaaaa cacggttgac ctgccgacgt gggaagaact
      721 gactgacatt cgcgacacac tgaatacggc aattgataag gagttgtccc gtacaaccag
      781 cgatgcgctg tttctggcgc tgcgccgggt gaaagcagat ctgaatg
//
LOCUS       DADLTF010000389          801 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.395, whole genome shotgun sequence.
ACCESSION   DADLTF010000389 DADLTF010000000
VERSION     DADLTF010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.395"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..244)
                     /locus_tag="JN749_004427"
     CDS             complement(<1..244)
                     /locus_tag="JN749_004427"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HAY7370552.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQSATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
     gene            complement(241..663)
                     /locus_tag="JN749_004428"
     CDS             complement(241..663)
                     /locus_tag="JN749_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HAY7370553.1"
                     /translation="MWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTP
                     PEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVR
                     ESNGEEQHFSVPFQTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT          222 a          219 c          151 g          209 t
ORIGIN      
        1 attgagtacc aaaatcacta atggatttac tataacgaat acgatattta ttcccataat
       61 ataaattcgc aatcgaacgc gtaatatctg tcgataaact ccctaacagc cccagagaaa
      121 tgccaatacc cgtagatatt gcattaaaat gagtggcgct ttgaatccct ccatacaccg
      181 tgacgccata aggaagcccg tacatcgccg tgatttcgcc cacggagaca ggggagcttt
      241 ttataccttc ttctttatat tcccccccat cacactatat ttgaaatatc cttcatgcag
      301 agcgatggcc ggcgtttgaa atggcacgga aaaatgctgc tcctcgccat tactctctct
      361 cactgtaaca tcaagctccc cgccaacggc cagcgttggt aaattatcca gcgcaaaagg
      421 ccctggcggc acagaacggg taaaaataat ttgcccattt tgtttaataa taacctgcgc
      481 ctcactttgt gctattccat gcaccacagg tgtgtagtag ctgtcctccg gcggagtcat
      541 cgcatcgtcg gtcgctaaca tcccgccgcg aaaaggtatg ctgtcaaaca cctcagacga
      601 cgtataagat tctccaagcg ttaatcggct tttaatcgca tttatgcctc gctcaaacca
      661 catgtaaccc ctttgccatc tttctgactg gccaagttcc ttgctccatg tcactgaact
      721 acgaaacctc caggcaccaa gattaaaccc tggagtgaac ctgccataac gagaattacg
      781 cgtggtagat tgattatcat t
//
LOCUS       DADLTF010000390          796 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.396, whole genome shotgun sequence.
ACCESSION   DADLTF010000390 DADLTF010000000
VERSION     DADLTF010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.396"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(135..671)
                     /locus_tag="JN749_004429"
     CDS             complement(135..671)
                     /locus_tag="JN749_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HAY7370554.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASQKMTKTAVNEALRRLKKAGIDKPELEAFLREMIEGKQKSWLTHCTDAEAL
                     KIDSVIGEVLADHPGLLNVLSQRYVGRGMSKRRMAELLNEQYPEWALITCRRRVDQWL
                     RVAEFILYSPMREAFDYA"
     gene            complement(668..>796)
                     /locus_tag="JN749_004430"
     CDS             complement(668..>796)
                     /locus_tag="JN749_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HAY7370555.1"
                     /translation="SSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
BASE COUNT          183 a          215 c          154 g          244 t
ORIGIN      
        1 catttttcag gcataaaaaa acccgctcaa tggcgggttc tggtaaagtt catgcgcttg
       61 gttcgcctcg cgatacagct ttgcgaagct gtacctgaat tgaagcagtt tgtggctcat
      121 tttgcaatga ttttttaagc ataatcgaac gcttctctca tcggtgaata caaaatgaac
      181 tcagcaacac gcaaccactg atcaacacga cgtcggcatg taatcaacgc ccactctggg
      241 tactgttcgt ttaataactc ggccatcctt ctcttactca tccctcgccc cacataacgc
      301 tgactcagga catttagtag tcctggatga tccgccagaa cttcacctat aacactatca
      361 atttttagtg cctctgcgtc agtgcagtga gttaaccagc ttttttgctt tccttcgatc
      421 atctcacgca aaaatgcttc caactctggt ttatcaattc ccgctttttt caatctgcgc
      481 agggcttcat tgacggctgt ttttgtcatt ttttgggatg ccagcaactg gttaaacata
      541 ttccctgacc tgccgccgcc aatatacgac cagcgccccc acatgcgcag ttttccctga
      601 atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc tgtatttgtt
      661 gggtaaatca caaatatccc tcctttctcc agatttcttg tgtgcgaaaa acaccttctg
      721 catgcatcag gcgtaattct tctttggtgt aatcgctggt ttttacccgc ccgtcgatta
      781 aatcgtggca tgagct
//
LOCUS       DADLTF010000391          782 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.397, whole genome shotgun sequence.
ACCESSION   DADLTF010000391 DADLTF010000000
VERSION     DADLTF010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.397"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..771
                     /locus_tag="JN749_004431"
     CDS             <1..771
                     /locus_tag="JN749_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HAY7370556.1"
                     /translation="PGNGKTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQL
                     HLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARRKIHDEDVRRPTEMTQEALRRI
                     AELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWIQLQRKTLSKHAEMAKAFDYIL
                     NHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKNYLFFGSDKGGESAAIIYSLLVTC
                     KQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
BASE COUNT          212 a          179 c          242 g          149 t
ORIGIN      
        1 ccggggaacg gaaagacgaa aacgggtcgt ctgtgggtat acgtcaggga tgatcgtaat
       61 gcgggttcat ccctgccggc agccgtctgg ttcgcgtatt cggcagatcg caaaggagaa
      121 catccgcagc tccacctggc aaagtatcag ggcgtactgc aggctgatgc ctatgcaggt
      181 tataacgtac tgtacgaaac gggccgggtg aaggaagccg ggtgcctggc ccacgcccgc
      241 cgaaaaatcc atgacgagga tgtgcgccgt ccgacagaaa tgactcagga agcgctcaga
      301 cggatagcag agttatacga catagaagcg gagatacgtg gcagtccggc agaggaacgg
      361 cttgcagtca gaaaagccag aagcgtccag ttgatgcagt cgttgtacga ctggatacag
      421 ttgcagagga aaacgctgtc gaaacatgcg gagatggcga aggcgttcga ctatatcctg
      481 aatcactgga atgcgctgaa cgagttctgt cgtgacggct gggtggaaat agacaacaac
      541 atcggtgaaa acgcgttacg atcggtggcg gttggaagaa aaaattatct ctttttcggc
      601 tcagacaagg gaggagaaag tgcggcgatc atctacagtc tgctggtcac ctgcaaacag
      661 aacgaagtgg agccggagga ctggttgcgc gaagtgatcg agaagctcaa tgactggccg
      721 tcgaaccaag tgcatgaact gctgccctgg aacttctcgt ctgtaaaata atccttacgc
      781 ta
//
LOCUS       DADLTF010000392          762 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.398, whole genome shotgun sequence.
ACCESSION   DADLTF010000392 DADLTF010000000
VERSION     DADLTF010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.398"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..83)
                     /locus_tag="JN749_004432"
     CDS             complement(<1..83)
                     /locus_tag="JN749_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HAY7370557.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGH"
     gene            complement(298..>762)
                     /locus_tag="JN749_004433"
     CDS             complement(298..>762)
                     /locus_tag="JN749_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAY7370558.1"
                     /translation="ASAALPLLFRPREVQGTMFGDGGMGGWRNMQGNTPVTPLVDAGC
                     NMVIVSHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGGLLSFTSAHTDAWRQ
                     QGYEDTMLTMEHIRKPLAARQALSRSETVLQKSLEITEEADLALRSAMARIK"
BASE COUNT          192 a          219 c          169 g          182 t
ORIGIN      
        1 tgatgtccgg cggtgctttt gccgttacgc accaccccgt cagtagctga acaggaggga
       61 cagctgatag aaacagaagc cactggagca cttcaaaaac accatcatac actaaatcag
      121 taagttggca gcatcaccta agtgctttta cgatgaattt catgataact ctccttatga
      181 cattaataaa tgcgttaatc ttaaatctca cccaggtaat atatatctga aaatattttc
      241 aacaacacca gacaggaaaa acaccaccac aggcggtggc gtttctttgg ggcgtcttta
      301 tttaatccgg gccatcgcgc ttctcagtgc caaatctgcc tcttccgtta tctccagact
      361 tttctgcaac accgtttctg accgggacag tgcctgacgt gctgccagcg gtttccggat
      421 atgctccatc gtcagcatcg tgtcctcgta gccctgctga cgccaggcgt cggtatgtgc
      481 cgacgtgaaa ctgagcagac cgccactgtt gccaccatca cctgcatatt tcagcctttt
      541 ccggggacgg atctcaagga ttgtggtgtc cggaaaagcc tggcgatccc ataaagaacc
      601 gtcactcaga tgcgacacaa tcaccatatt gcatccggca tcgaccagag gcgttacagg
      661 ggtatttccc tgcatatttc gccatcctcc cataccacca tcaccgaaca ttgtcccctg
      721 aacctcacgg ggacggaaca acaggggcag tgcggctgac gc
//
LOCUS       DADLTF010000393          750 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.399, whole genome shotgun sequence.
ACCESSION   DADLTF010000393 DADLTF010000000
VERSION     DADLTF010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.399"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(12..374)
                     /locus_tag="JN749_004434"
     CDS             complement(12..374)
                     /locus_tag="JN749_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAY7370559.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKASTTAIRYMLYVGFMGILSAIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          225 a          144 c          167 g          214 t
ORIGIN      
        1 aagagatatt ttcatttcgc atccctaaaa acaatgaact ttgaatagac gaaaccgcac
       61 accagactga tggcggagaa ggtgataaga gtaactattg gaggaagtga gcatttatca
      121 gcagcccaac caacaatcgc actcaatatt cccatgaatc ccacgtataa catgtagcgt
      181 atcgctgtag tcgatgcttt gaatgtgaat cttgcattcg cgaagaagct aaagctcaca
      241 gctactacga aacctgtgaa gtttgccagg gcctgactgg tatgcgcggc atagatacat
      301 acaccaaaaa ctacccagtg tataagtgta ttcagcacac ctatagatgt gtactttaca
      361 aataacttta acatttattt aatcaatgag ttctgaaagt catgaagtct atcatccaag
      421 tctcaattga tcgatatttg ctgtgtctga tgagacaaaa ctgagacaca taaggcctcg
      481 caatggcttg caaggcttta catgttttga tttagtggga cgtgtgagcg cagtgttgat
      541 ggggtaatgc tttgaattag aagcggattc ttataattcg taatgcgaag gtcgtaggtt
      601 cgactcctat tatcggcacc atttaaatca ataagttaca catcattagt accttcctta
      661 tttcttgact gggacaaatt tgggaccgat gggttcagaa tcgagtctat ttgccgtgcg
      721 tgttcggtaa ggtgattagg tgcgaggtga
//
LOCUS       DADLTF010000394          749 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.400, whole genome shotgun sequence.
ACCESSION   DADLTF010000394 DADLTF010000000
VERSION     DADLTF010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.400"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>749
                     /locus_tag="JN749_004435"
     CDS             <1..>749
                     /locus_tag="JN749_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7370560.1"
                     /translation="IGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEAKTDARDAAI
                     IAEAARTLPHALRTLKLADEQIAELSILCGFDDDLAAQTTQASNRIRGLLTQIHPALE
                     RVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAE
                     QTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVR
                     TAARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT          146 a          257 c          196 g          150 t
ORIGIN      
        1 catcggtgcg ttacctgtcg ccgttgcccg ctcagaagga gtccttgtcg gatacctccc
       61 tggactggcc atgcgccgca tagccgactt acacgccggt gaagctaaaa ctgatgctcg
      121 tgacgctgcc atcattgccg aagctgcccg taccctacct cacgcgctac gcacgctgaa
      181 actggctgac gagcaaatcg ccgaactctc cattctctgc ggcttcgatg atgatcttgc
      241 cgcacagaca acgcaggcca gcaaccgtat ccgcggcctt ctgacccaga tacatccggc
      301 actggagcgc gttctcggtc cgagacttga gcacccggcg gtactcgatc ttctccagcg
      361 atatccctca ccagaaaaac tcgcttcgct gggtgagaag aagctggcag cccagctctg
      421 caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac atagctcagg cactggccga
      481 acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta gtactgccac gtctggcact
      541 ccagctcatc acgctgcgta agcaaagaga cgaggtggcg cttgaggtag aacagcgagt
      601 tcttgctcac cctctttacc cggtcctgac cagtatgccc ggagtcggtg tcaggaccgc
      661 agccagactc ctcaccgagg tcgcctgccg cgccttcgcc tctgccgcac atctcgctgc
      721 ttatgctggc cttgcgccgg taactcggc
//
LOCUS       DADLTF010000395          742 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.401, whole genome shotgun sequence.
ACCESSION   DADLTF010000395 DADLTF010000000
VERSION     DADLTF010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.401"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..255
                     /locus_tag="JN749_004436"
     CDS             <1..255
                     /locus_tag="JN749_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7370561.1"
                     /translation="NDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDK
                     EKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            279..>742
                     /locus_tag="JN749_004437"
     CDS             279..>742
                     /locus_tag="JN749_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAY7370562.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGT"
BASE COUNT          198 a          172 c          216 g          156 t
ORIGIN      
        1 aatgatgaat ttgtctttga ctacagactc aaagcggaag atgagcgtga tgcccttctg
       61 gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgctgctggg gttaatcagc
      121 gacgaagata aagaaaagct gaaagcctgt cgtatttacg cgaaatcgct gcaggcgatg
      181 gatttcagca ccatcactga taaatcctca tacaacgcca ttgaatggcc cgcctctccg
      241 gaaggttctt cctgatttaa tttatcgcga gaaaaacaat gtctgtagtg atatcaggtg
      301 cgctgactga tggcgcaggt atccccatgt ccggatacca tattattctg aaatcccggg
      361 taaacacccc ggaagtggtg atgaacactg ttgctgatgt gatgacagga aacgatggtg
      421 aatactgttt ccatgcgcgg actggaaaat atggtgtgta tctgaaacag gactggcgca
      481 acgagtacaa cgttggcgac attgctgtat atgaggactc aaagcccggc acgctgaatg
      541 actttctgat tgctcctgat gagggcgacc tgaaaccgga tgtcgtcaaa cgctttgagg
      601 aaatggtggc gcaggcgcag cagagcgccg gggccgcagc cggaaacgca cagcagacgg
      661 cgcaggatgt ggcggcagcc gcaggttatg cccgcgcagc agagcaggcc aaaaatgaca
      721 ttgatgctgc gctgaccggc ac
//
LOCUS       DADLTF010000396          730 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.402, whole genome shotgun sequence.
ACCESSION   DADLTF010000396 DADLTF010000000
VERSION     DADLTF010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.402"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..200
                     /locus_tag="JN749_004438"
     CDS             <1..200
                     /locus_tag="JN749_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370563.1"
                     /translation="PTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLKL
                     DEGSNTASGLGKKYWTEICVR"
     gene            188..355
                     /locus_tag="JN749_004439"
     CDS             188..355
                     /locus_tag="JN749_004439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7370564.1"
                     /translation="MRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSVNPGLV
                     RASATFTLEFQ"
     gene            368..>730
                     /locus_tag="JN749_004440"
     CDS             368..>730
                     /locus_tag="JN749_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HAY7370565.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKN"
BASE COUNT          211 a          161 c          176 g          182 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat
//
LOCUS       DADLTF010000397          728 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.403, whole genome shotgun sequence.
ACCESSION   DADLTF010000397 DADLTF010000000
VERSION     DADLTF010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.403"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(55..606)
                     /locus_tag="JN749_004441"
     CDS             complement(55..606)
                     /locus_tag="JN749_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HAY7370566.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQPDYKIAYE"
BASE COUNT          211 a          148 c          140 g          229 t
ORIGIN      
        1 ccacttatca ttaatccgtt aatgatgtgg ccgggcgacc ccggcctaat gtgattactc
       61 gtaagcaatc ttatagtcag gctgagcgta aacgttgccc ggggtagcat ctttgcccgg
      121 ggattccata cgagctttga agttcagttc ctgatagctg ttagtattgt tgaatgtaat
      181 tactaccgga gtacctaaag tcacttcgcc gctctggcta tccagaatac gtacgccaac
      241 gcctgtagca ccaccgattt cagtttcttt ggtgtttttc agcaggtcag tctgacccgt
      301 catagtattc gctgtggtga aagtaacctt cgcttttgaa taagtggttc caccctggct
      361 caacatgcag tcttgcaggt gaatagtgaa aggttcagcc aaagatttct gatcgctttt
      421 cagtacggta tctgctactt cacccagatt tacagttaaa tcttcgtcac ctggtttaat
      481 agaacatgga gcagagataa cttcaccttt aaatttaaca ataccgtgac cttcatcagc
      541 aaacacgtta ttggaaagaa tcattgctac accagcaaca agaagtgact ttttaaaagc
      601 agacataata ttcctttata taaagataag taaaaaagag aatgattagt aactgttatt
      661 taaggactac caatcattaa aatattatat tttgttttgt gatttttata aaattagttt
      721 atctaaat
//
LOCUS       DADLTF010000398          725 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.404, whole genome shotgun sequence.
ACCESSION   DADLTF010000398 DADLTF010000000
VERSION     DADLTF010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.404"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..452
                     /locus_tag="JN749_004442"
     CDS             <1..452
                     /locus_tag="JN749_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370567.1"
                     /translation="NRKKELTEPQIREGSWRKERRTSYSREFKVRLAKQALQPGAVVA
                     RIAREHDINNNLLFKWKSQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNK
                     HDERPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            449..>725
                     /gene="tnpB"
                     /locus_tag="JN749_004443"
     CDS             449..>725
                     /gene="tnpB"
                     /locus_tag="JN749_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAY7370568.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQ"
BASE COUNT          187 a          185 c          209 g          144 t
ORIGIN      
        1 ccaacagaaa gaaggaatta acagagcctc aaatcagaga aggctcatgg cgaaaagaac
       61 ggcgaaccag ctatagccgt gaatttaagg tccgtctggc taagcaggcg ttacagcctg
      121 gggctgttgt tgcccggatc gccagagaac acgatatcaa taataacctg ctgtttaaat
      181 ggaaaagcca gtacgaggac ggcttactga gcgatgatga catacaggaa tgcatgcctg
      241 tcccggtggc actgactgat acgccggagc cgaccagacc agttacaaat cccttctggc
      301 gtaacaagca tgatgagcgc cctgaggggg ctcccggaaa cgtcccacgg tgcgagctgc
      361 atcttaaatc aggtgtggta aaactgtttg accctctcac tccggaactg ttacgggcgc
      421 taatccgcga aatgaaaggg ggtatccgat gataacgctg ccgaccggta ccaaaatctg
      481 gatcatcgct ggcatcacag atatgcgttg tggcttcaat ggcctggctt cgaaggtgca
      541 gaacacgctg aaagatgacc cgttctccgg gcatatcttc gtcttccggg gccgcagtgg
      601 caaaatggtg aaaatactgt gggccgatcg tgacgggtta tgcctgttcg ccaaacgcct
      661 ggaacggggc cgcttcgtct ggccggtgac ccgggaaggg aaagtgcacc tgacgccagc
      721 tcagt
//
LOCUS       DADLTF010000399          712 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.405, whole genome shotgun sequence.
ACCESSION   DADLTF010000399 DADLTF010000000
VERSION     DADLTF010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.405"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            278..>712
                     /locus_tag="JN749_004444"
     CDS             278..>712
                     /locus_tag="JN749_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HAY7370569.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLA"
BASE COUNT          217 a          123 c          147 g          225 t
ORIGIN      
        1 atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata
       61 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
      121 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
      181 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
      241 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
      301 gtttataact gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac
      361 ggatggctca gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      421 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      481 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      541 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      601 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      661 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cg
//
LOCUS       DADLTF010000400          702 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.406, whole genome shotgun sequence.
ACCESSION   DADLTF010000400 DADLTF010000000
VERSION     DADLTF010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.406"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..500)
                     /locus_tag="JN749_004445"
     CDS             complement(<1..500)
                     /locus_tag="JN749_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HAY7370570.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLLRCTG"
BASE COUNT          214 a          177 c          133 g          178 t
ORIGIN      
        1 ccagtacacc tcaacaagac ctgatgcaaa tcaacgtcct tttgccattc ataaacatca
       61 tctggataat cggcatgagc aataacatgc ttgccggtgc gactgtgtac ttcaagaata
      121 aagggcaaat gctgacattt ttccagcgcc gttttcgctt gtttctgttg attatctgcc
      181 agcgcaataa accagtcgcc gccattcatc aaccacaaag acatctgctg gaatgccagc
      241 gcatccatcg ccatctgttc atgattgcct cttaccgcac gaacccaatg ttgttccagt
      301 aactgcagac aacgtaaact ttgcggccca cgatcgataa cgtctcccac tgagataagt
      361 aaatctcgcc acggatcaaa acgacaatgc cataatttgc ggcgcaactg ctcaagacaa
      421 ccgtgtatat cgccagaaag ccagatatgt cgccattgat gacccgcaat tctctgataa
      481 acgggcgcag gctgtttcat caatattttc ctcccgcgct aaagatcaca taatcttaac
      541 aagaatgtta aaaaacgctg gactcagaca gtagagtgtg tgttatggtt gactataaag
      601 tcagcgaagg aaatgcttct ggcttttaac agataaaaag agaccgaaca tgattcctgt
      661 tttcgtcaac aaacaataaa accctttaaa attaatgcgt tg
//
LOCUS       DADLTF010000401          697 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.407, whole genome shotgun sequence.
ACCESSION   DADLTF010000401 DADLTF010000000
VERSION     DADLTF010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.407"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..>697)
                     /locus_tag="JN749_004446"
     CDS             complement(<1..>697)
                     /locus_tag="JN749_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HAY7370571.1"
                     /translation="LRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKKRIGEI
                     YHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGEVGQTA
                     PNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMV
                     ENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVE
                     CFFGTLKSECFYLD"
BASE COUNT          182 a          167 c          142 g          206 t
ORIGIN      
        1 catcaagata aaaacactcc gactttaagg ttccaaagaa acactccacc acagcattat
       61 ccagacaatt gccttttctg gacatgcttt gtttaatacc atgttcttta aggatatttt
      121 gatatcttct catacgatac tgccatccct ggtcagagtg cagaacagga tgctcgtgag
      181 gattaagctt tttgaatgcc tgatcgagca tattctcaac catgttcatc actggtcttt
      241 ccgaaaggct gtaagaaata acttcgttgt tgaagagatc tattactgga gacaaataca
      301 gcttgcgccc attgactgca aattcagtaa catcggtaac ccacttctcg tttggccgcg
      361 tagccttgaa atctctttgg agaacattag gggcggtttg ccctacctct cctctgtaag
      421 agcggtatcg cttgacctta atcgctgctt taagtgagag ggttcccatc aggcgctgaa
      481 cagctttatg gttaatctgt ttcccttctc gatgaagaga cagcgttacc ctacggtatc
      541 cgtatcggcc tctattctcg tgataaatct caccaatacg ctttttaacg tccgcatact
      601 tgtcaggctt gctgagagcc tttagatggt aataaaacgt actgcgcggt atctccgcag
      661 ctctgagaag ctcatcaaga ggataaaact gccttag
//
LOCUS       DADLTF010000402          692 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.408, whole genome shotgun sequence.
ACCESSION   DADLTF010000402 DADLTF010000000
VERSION     DADLTF010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.408"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            36..>692
                     /locus_tag="JN749_004447"
     CDS             36..>692
                     /locus_tag="JN749_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAY7370572.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHHNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     P"
BASE COUNT          160 a          177 c          182 g          173 t
ORIGIN      
        1 agggggtaat ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg
       61 aaattaaaat cctgcacaag cagggaatga gtagccgggc gattgccaga gaactggggc
      121 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
      181 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      241 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      301 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      361 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      421 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      481 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      541 ataatgcgtt ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa
      601 ctgtggttct gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt
      661 ggcagttcgg caaggagatg ggcttctctc cc
//
LOCUS       DADLTF010000403          692 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.409, whole genome shotgun sequence.
ACCESSION   DADLTF010000403 DADLTF010000000
VERSION     DADLTF010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.409"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            57..554
                     /locus_tag="JN749_004448"
                     /pseudo
     CDS             57..554
                     /locus_tag="JN749_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            595..>692
                     /locus_tag="JN749_004449"
     CDS             595..>692
                     /locus_tag="JN749_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7370573.1"
                     /translation="MLHEEKLARHQRKQAMYTRMAAFPAVKMFEEY"
BASE COUNT          176 a          167 c          208 g          141 t
ORIGIN      
        1 gccagccccg tcccgcccga tagagcgcgg catagcgggc cccggattac ttgcgtgcga
       61 cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat gaaatacggg
      121 gattaccaga gtcagagtgt cttgcagtca ggcagcagcg aagcaaagcg ttactgacgt
      181 cgctgcatga atggatggtg gagaagaatg gcacgctgtc gaaaaaatcc agactgggcg
      241 aagcattcag ctatgtactg aatcagtggg acgccctctg ttattacagt gatgacggtc
      301 tggcggaggc ggacaataac acagcggaaa gagcgcttcg tgcagtctgt ctcggaaaga
      361 aaaattacgt gttcttcggt agcgatcacg gcggcgagcg tggtgcactg ctgtacgggc
      421 tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc catattctga
      481 gcgtactgcc ggaatggcct tccaaccgag ttgacgacct cctgccatgg aaggtggtgc
      541 taccatcagg ataatcgtca atacggtgtt cggtgaacgt ttacttggag catcatgctt
      601 catgaagaaa aactggcacg tcatcaacgt aaacaggcga tgtatacccg aatggcagcc
      661 ttcccggcgg tgaaaatgtt cgaagagtat ga
//
LOCUS       DADLTF010000404          691 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.410, whole genome shotgun sequence.
ACCESSION   DADLTF010000404 DADLTF010000000
VERSION     DADLTF010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.410"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..246
                     /locus_tag="JN749_004450"
     CDS             <1..246
                     /locus_tag="JN749_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370574.1"
                     /translation="NIYGHHFGAPHDAADFRRCVALVEQIPEIRDSFDKVAKRVPAFK
                     GILNEWDSLVDLLKSEMKIHGNKAPETYRRISELRKD"
     gene            337..684
                     /locus_tag="JN749_004451"
     CDS             337..684
                     /locus_tag="JN749_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAY7370575.1"
                     /translation="MEEEIFTREEAASYLKVDKGTITQWIRSGRLQAVKINPDKPKSP
                     YRICKSDCIAALKSVRHNSAVNAVDVQEVKACQSNYAVARGTAISSRQMDQELDALLK
                     HRTKGKRKNFTIV"
BASE COUNT          216 a          160 c          168 g          147 t
ORIGIN      
        1 aatatctatg gtcatcactt cggcgctccc catgacgcag ccgatttcag acgatgcgtt
       61 gcacttgttg agcagatccc agaaatcaga gattcattcg acaaggttgc aaagcgcgtt
      121 ccggcattca aaggcatcct caacgaatgg gattccctcg ttgatctgtt gaagtctgaa
      181 atgaagatac acggaaacaa agcaccagag acttacagaa gaattagcga gttacgcaag
      241 gactaaccac agcctcatac tcgatgaggc ctgttcattg ctcaatgata tccagaccta
      301 ccgcaataat accaatccaa taaatggaga ttccaggtgg aagaagaaat cttcactcgt
      361 gaagaggcag cgtcgtatct gaaggtagac aaaggcacta tcacgcagtg gatacgaagt
      421 ggacgacttc aggccgtaaa gataaatcca gataaaccta aaagcccata tcgcatttgc
      481 aagtcagact gcattgcggc gcttaagtct gtgagacaca atagcgcggt gaatgcggtt
      541 gatgtgcagg aggttaaagc atgtcaatca aactacgcgg tggcacgtgg cactgcgatt
      601 tcgtcgcgcc agatggatca agagttagac gctctcttga aacatcggac aaaaggcaag
      661 cgcaagaact tcacgatcgt ctgaaagcag a
//
LOCUS       DADLTF010000405          686 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.411, whole genome shotgun sequence.
ACCESSION   DADLTF010000405 DADLTF010000000
VERSION     DADLTF010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.411"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..573
                     /locus_tag="JN749_004452"
     CDS             <1..573
                     /locus_tag="JN749_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA
                     acyltransferase"
                     /protein_id="HAY7370576.1"
                     /translation="RHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFHQQRNPVID
                     YVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFADFFATYKA
                     TLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTIARQMNKTV
                     EILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          211 a          139 c          158 g          178 t
ORIGIN      
        1 cgacacaata atagaaatgt gatttttctg gttccccatg gctggagcgt ggatattcct
       61 gcaatgttgc tggcagctca gggaaaaaaa atggccgcca tgtttcatca gcaacgaaat
      121 ccagtgattg attatgtctg gaattcagta cggcgtaaat tcggggggcg cttacatgcc
      181 cgggaggatg ggataaaacc atttattcag tcagtacgcc agggatactg gggatattac
      241 ctcccagatc aggatcatgg tcctgaatac agtgaatttg ctgatttttt tgcgacctat
      301 aaagcgacat taccaatcat tggacgtctg atgaacatca gtcaggctat gattatacca
      361 cttttcccgg tttatgatga aaaaaaacat ctactgacta ttgaaattcg gccaccaatg
      421 gatgcttgca ttgccagcgc ggacaataaa acgattgccc gacaaatgaa caaaacagtg
      481 gaaattttgg tggggccaca tccggaacag tatgtctggg ttttaaaatt gttaaaaaca
      541 cgcaaatcaa acgaagcgga cccgtaccct tgaaattata aagtacaagt atttccacta
      601 gttgtttgta agcgtacagc ctgaaccgtc tggtcagaat ctgacgaatt agacaaagtg
      661 gtgtccacca aataagtagt gggaac
//
LOCUS       DADLTF010000406          685 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.412, whole genome shotgun sequence.
ACCESSION   DADLTF010000406 DADLTF010000000
VERSION     DADLTF010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.412"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..170)
                     /locus_tag="JN749_004453"
     CDS             complement(<1..170)
                     /locus_tag="JN749_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAY7370577.1"
                     /translation="MKSMDKMSTGIAYGTSAGSAGYWLLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNL"
     gene            complement(283..519)
                     /locus_tag="JN749_004454"
     CDS             complement(283..519)
                     /locus_tag="JN749_004454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370578.1"
                     /translation="MAITSALQADDAGSIPAAAQVKVFQSAMMGKHGVTERRLRFECS
                     SFLQNCCAEIPTFGQAIIHKHSAFIPINCGWFVG"
     gene            complement(463..533)
                     /locus_tag="JN749_004455"
     tRNA            complement(463..533)
                     /locus_tag="JN749_004455"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(498..500),aa:Val,seq:tac)
     gene            complement(537..612)
                     /locus_tag="JN749_004456"
     tRNA            complement(537..612)
                     /locus_tag="JN749_004456"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(577..579),aa:Thr,seq:tgt)
BASE COUNT          203 a          184 c          159 g          139 t
ORIGIN      
        1 tacagattcg tcagataagt cagaagcccc agaagcagac ttcccagcac accaatcgca
       61 gcccactgtg acggactgac ctgatcaagc cactgtaaaa gccagtagcc ggcactgccg
      121 gcggaggtgc cgtaggcaat gcccgttgac attttgtcca tagatttcat aacctcacct
      181 ccgcaaataa cggatggcgt agctttacac tgagagacga aaaggatttg aaaaaaagcc
      241 cgcaaaagcg ggcgcaacgg aatctacagt aaggaaagca ctctatccaa caaaccaccc
      301 acagttaatc ggaataaaag cagagtgctt atgaatgatc gcctgcccga aggttggtat
      361 ttctgcacag caattttgca aaaaagacga gcattcaaaa cgcaaacgtc tttcagtcac
      421 tccgtgcttt cccatcatcg cagactgaaa gactttaact tgagcggcag cgggaatcga
      481 acccgcatca tcagcctgta aggctgaggt aatagccatt atacgatgcc cgcatatggt
      541 gccgactacc ggaatcgaac tggtgacctg atgattacaa gtcagttgct ctgcctgctg
      601 agctaagtcg gcgctagccc accaccgaga actcgaacct cgcaccgtca acttagaagg
      661 ttgatgctct atccggatga gctag
//
LOCUS       DADLTF010000407          666 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.413, whole genome shotgun sequence.
ACCESSION   DADLTF010000407 DADLTF010000000
VERSION     DADLTF010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.413"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..276
                     /locus_tag="JN749_004457"
     CDS             <1..276
                     /locus_tag="JN749_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAY7370579.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESAA
                     IIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(521..>666)
                     /locus_tag="JN749_004458"
     CDS             complement(521..>666)
                     /locus_tag="JN749_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370580.1"
                     /translation="NRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQR
                     LRN"
BASE COUNT          159 a          155 c          174 g          178 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttctga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg gtcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatctag gttgtgtccc ttaattacgt aagcgttgat
      541 aaaagagtcg gatgcagccc agtttcacca tcgccaggta atttctcgcc gttttgtcgt
      601 aacgcgtggc gatgcggcga tattctttca gccgcccaaa gcaccgttca acgacattac
      661 gattgc
//
LOCUS       DADLTF010000408          663 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.414, whole genome shotgun sequence.
ACCESSION   DADLTF010000408 DADLTF010000000
VERSION     DADLTF010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.414"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>663
                     /locus_tag="JN749_004459"
     rRNA            <1..>663
                     /locus_tag="JN749_004459"
                     /product="23S ribosomal RNA"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ccctttaagg gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DADLTF010000409          661 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.415, whole genome shotgun sequence.
ACCESSION   DADLTF010000409 DADLTF010000000
VERSION     DADLTF010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.415"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            105..>661
                     /locus_tag="JN749_004460"
     CDS             105..>661
                     /locus_tag="JN749_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7370581.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLMVSGNRLDS"
BASE COUNT          236 a          121 c          112 g          192 t
ORIGIN      
        1 gaagcaatac ctggagaaag agtacaaagc aaactgtatt aaacttaata agttatattg
       61 tatttcacta gagttcttat cgttgataag aaattctggt tataatgatt aaatcaacaa
      121 atatacaggt aatcggttct ggtattatgc atcaaataaa caacatacac tcgttaactc
      181 tattttcttt gcctgtgagt ttgagtccat cttgtaatga atattattta aaagtctgga
      241 gtgaatggga aaagaacggt accccaggcg agcaacgcaa tatcgccttc aataggctaa
      301 aaatatgttt gcaaaatcaa ggggcggaat taaatctatc tgagttagat ttaaaaacat
      361 tacctgattt accgcctcag ataacaacac tggaaataag aaaaaaccta ttaacatatc
      421 tccctgattt cccaccaatg cttaaggtaa tacatgctca atttaatcaa ctggaaagct
      481 tacctgcctt gcccgagacg ttagaagagc ttaatgtggg tgataacaag ataaaagaat
      541 taccatatct tcctgaaact ctaactcatt tacggatcca taataaccga ttgcatattc
      601 tgccactatt gccaccggag ctaaaattac tgatggtttc tggaaacaga ttagacagca
      661 t
//
LOCUS       DADLTF010000410          658 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.416, whole genome shotgun sequence.
ACCESSION   DADLTF010000410 DADLTF010000000
VERSION     DADLTF010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.416"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(152..658)
                     /locus_tag="JN749_004461"
     CDS             complement(152..658)
                     /locus_tag="JN749_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HAY7370582.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          160 c          146 g          179 t
ORIGIN      
        1 cgtttaacgc cctgtgccat ttttcaggca caaaaaaacc cgcataaagc gggttctttc
       61 aagtgtccat gtctgctatt cgcctcgcga tacagctttg cgaagcttac cggaattgaa
      121 gcagtttatg tgcaaaaatg caagaacttt tttaaagctg catcaacctt tccaccagtt
      181 tatctctgcg aacaacaaac caaccattgg ctctcgccag ttccagccat gactcaaggg
      241 aaataacaat atcatcatcc cgtaactgaa ttgtggaaac agtgacaccg cctctctgat
      301 aacagagaac tcgcgtgtcg taacttttct ggcatgaaac tggcgttgac ggatcccttt
      361 gactgaaata gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca
      421 ttttggcgtg gcgtgctgcg cctcgttctg tccagaggat gagggagcgg gctttgggtg
      481 aaactggatt ttgtgagtag tttaaagcga cccgcaattc tttaaggtca ttaccaacaa
      541 ctttgaaaaa gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa
      601 tacgcaccgg ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acaggaat
//
LOCUS       DADLTF010000411          653 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.417, whole genome shotgun sequence.
ACCESSION   DADLTF010000411 DADLTF010000000
VERSION     DADLTF010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.417"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..578)
                     /locus_tag="JN749_004462"
     CDS             complement(<1..578)
                     /locus_tag="JN749_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HAY7370583.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAA"
BASE COUNT          167 a          164 c          155 g          167 t
ORIGIN      
        1 gcggccacat tttcgacttc ctccggagag gaaaatttac ggaacaggat ccagttccac
       61 agcacattca gtacagattt ataaacctgc tgaaactcgg tttcgtccat gttcgcaaat
      121 gcgatagatt ttgccctgcg cccacgacta ccatcaggat aaatatgctc ggtgtaaaat
      181 ccggcctgaa tggttaccca ctcgcggaaa gcgtcaaacg actttagcaa tgccgtatcc
      241 cgggttctgc atgtcgcaac tgtattaagg tattgctctg cggcatcact cagggctgga
      301 gtgtgttccc gacctactga ttcgcacagg taatcaacga agcctgatac cagttttcgt
      361 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat caaatcccag ttgcaggagt
      421 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      481 tcacctattt tgatttgatg caaaaaatcg caactctccg gcgtcgccgg gagaagtaat
      541 ccggaagagg tttgtttgac cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      601 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taa
//
LOCUS       DADLTF010000412          652 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.418, whole genome shotgun sequence.
ACCESSION   DADLTF010000412 DADLTF010000000
VERSION     DADLTF010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.418"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            29..373
                     /locus_tag="JN749_004463"
     CDS             29..373
                     /locus_tag="JN749_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370584.1"
                     /translation="MDTSRDNSELFANDGTPTWLKEKRAKEIALDEIIELLNKHHGGT
                     SNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGVEYAFPDPQTQNE
                     TQQAGYDENIPV"
     gene            424..612
                     /locus_tag="JN749_004464"
     CDS             424..612
                     /locus_tag="JN749_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HAY7370585.1"
                     /translation="MYKITATIEKEGGSPTSWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
BASE COUNT          236 a          129 c          144 g          143 t
ORIGIN      
        1 tcttaacctg ggaggtgaac accccggcat cgatacctca cgggataaca gtgaattatt
       61 tgccaacgac ggtacaccga catggctaaa agaaaagcgt gcaaaagaaa tcgcacttga
      121 tgaaattatc gagttgctca acaagcacca tgggggcacc agtaacgacg ccaaacgcgc
      181 aaaagctgat ttgctggaaa aaacattcac ctcccgctca tgggaaagaa tcaaaggtat
      241 ggactggccg acaatcaaag ctggtcgtaa tgcactatgg attgaactgg aaggcgttga
      301 atatgcattc ccggatccac aaacacaaaa cgaaacgcaa caagcaggat acgatgagaa
      361 tattccggtg taaaaccaag tgcattttga acacggtttt tgaaaatatt aatggaggtg
      421 ttcatgtata aaattacagc cactattgaa aaggaaggtg gttctcctac cagctggaca
      481 agatattcaa aatctaaact aacgaaatca gaatgcgaaa aaatgctctc agggaaaaaa
      541 gaagcaggcg tttccagaga gcagaaagta aaactgataa attttaattg cgagaaactt
      601 ctgtcctcgt gagttgcatt atatcaaaat tagaacttca tagctgattg ag
//
LOCUS       DADLTF010000413          613 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.419, whole genome shotgun sequence.
ACCESSION   DADLTF010000413 DADLTF010000000
VERSION     DADLTF010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.419"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JN749_004465"
     CDS             complement(12..>613)
                     /gene="ospZ"
                     /locus_tag="JN749_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HAY7370586.1"
                     /translation="HGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYMKT
                     MYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEISC
                     KIFSKENSDFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIKSE
                     IIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          185 a          122 c           91 g          215 t
ORIGIN      
        1 attaatatac attaagtaac aggcattcga gccttgattt gagaaagcct gtcattcatt
       61 tgttttatgt agctatctat ttcctctgat gcataactaa aaagttctcc catttcacga
      121 gcaataataa tctcagattt aatagacttt aactctggcg actcatctct taacacttgc
      181 ataatctctc tttttatatc atatagttct ataaaccgat ttgcccgtag ttctccttgg
      241 ggagaatcat ttctgaatac aacaattata tacaaaaaat cactattttc tttagagaag
      301 atcttacaac taatctctgg gagctcacat aatgcatgta agtcaggatg gtacaaacta
      361 tccacagaaa attgccccat aggacgtttt ccagatataa ctgtcaacca gcttccttcc
      421 ggtgtactgg cttcgtctct ggaaataggc tgaaaatctt ccggacatgg agaatacatt
      481 gtcttcatat aattaagtcg tctggatttc tcattaataa actcctctat ttttaattgt
      541 gccatacttc tggttatacc agagatatac ttcttgtcat atatgtttgg tgattcatta
      601 aagtaaccat gtt
//
LOCUS       DADLTF010000414          612 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.420, whole genome shotgun sequence.
ACCESSION   DADLTF010000414 DADLTF010000000
VERSION     DADLTF010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.420"
                     /strain="EG0002"
                     /db_xref="taxon:623"
BASE COUNT          221 a          109 c          128 g          154 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catatcaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa at
//
LOCUS       DADLTF010000415          595 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.421, whole genome shotgun sequence.
ACCESSION   DADLTF010000415 DADLTF010000000
VERSION     DADLTF010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.421"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..449)
                     /locus_tag="JN749_004466"
     CDS             complement(<1..449)
                     /locus_tag="JN749_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7370587.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSKLREAVATERLTVDQHRYINDVIGVLKACQDRLNG"
BASE COUNT          151 a          128 c          156 g          160 t
ORIGIN      
        1 ccattgagtc tatcctgaca ggccttaagc acaccgataa cgtcattaat atagcgatgc
       61 tggtctacag tcagacgctc ggtcgctaca gcttcccgaa gtttggactt aatgtcatcg
      121 tactggtcaa cgtaaagctg gtaggactcc gggtcgttca ggtcatcacg gctgacacta
      181 agttcccact cgacaatggt cttcaggtgc tcccgtagct cctcataaga ggcgttaggg
      241 ttgtctgcat ggattgcttt aagtgctgac ttgatgtgcc gtgagtgagc catagcggta
      301 cgtctatcct tggtccgtaa agatactgac gctagtttat aacggttgtg agaaggaagc
      361 gaagcggtca ggcgactagt ggtcttcatc ctaagacgga tgtaaaagac accattacgc
      421 tgataaaggt aggttcttga cttgagcatg tactgagacc ttaggtctgc tcactacttc
      481 aacaaagtta gtcacagtgt tggttacagt gagtaaaact atcggaaggc gttaagccgt
      541 atcccttagg attcagtaag ttagtagact ttaagaagtg tcatggagcg ggcga
//
LOCUS       DADLTF010000416          568 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.422, whole genome shotgun sequence.
ACCESSION   DADLTF010000416 DADLTF010000000
VERSION     DADLTF010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.422"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            31..318
                     /locus_tag="JN749_004467"
     CDS             31..318
                     /locus_tag="JN749_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005046668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAY7370588.1"
                     /translation="MFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEMYYSPGSNT
                     VSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVASI"
     gene            378..>568
                     /locus_tag="JN749_004468"
     CDS             378..>568
                     /locus_tag="JN749_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370589.1"
                     /translation="MTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRVVWVG
                     KDVTGIEPRNASVIEVPDIT"
BASE COUNT          175 a          117 c          146 g          130 t
ORIGIN      
        1 ttcagccaga gaaacccgga taacagcata gtgttcactg ttaacggaca ggtaattccg
       61 ttaaattacg gaaacttcga tgcccgctat aaatatcgaa cagagggggt acaggatgta
      121 cggtatggcc atgaaatgta ttacagcccc ggcagtaaca ccgtttcgtg gagattttgc
      181 gcaccttcgg gacacgggct gtcagggatg gcgatatcgg ataccggccg taactcagcg
      241 gataacgttg acggtgtgta ttaccgacca ctgcaaaaac tgattaatgg cacctggtat
      301 aacgtagcga gtatttaaca atgttgcatt taaaaaatat tactgcgggt aatccgaaaa
      361 cagcagaaca atatcagatg acaaaacgat attcggtcac ctggcttttt tcagaagacg
      421 gaaaaaactg gtatgaagag ctgaagaatt tcgccagcga cacaataaaa atagcttaca
      481 ccggagatgg tcgcgtggtg tgggtcggta aggatgtgac aggcatcgag ccacgcaatg
      541 ccagcgttat cgaagttccg gatattac
//
LOCUS       DADLTF010000417          566 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.423, whole genome shotgun sequence.
ACCESSION   DADLTF010000417 DADLTF010000000
VERSION     DADLTF010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.423"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..297
                     /locus_tag="JN749_004469"
     CDS             <1..297
                     /locus_tag="JN749_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HAY7370590.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="JN749_004470"
     CDS             complement(405..>566)
                     /locus_tag="JN749_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370591.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DADLTF010000418          555 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.424, whole genome shotgun sequence.
ACCESSION   DADLTF010000418 DADLTF010000000
VERSION     DADLTF010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.424"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>555
                     /locus_tag="JN749_004471"
     CDS             <1..>555
                     /locus_tag="JN749_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HAY7370592.1"
                     /translation="VNMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNEAFNRELAKLN
                     QSLRETLSKFNKTTSKNNYSENASNSLDVLATSAIKAGTAIYGFQTALEAYRKVMEIG
                     LKKEASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETFSSFAKFSAGAGDMNADQ
                     KESLFSNVIGTSRLMGLSTDEING"
BASE COUNT          187 a           87 c          121 g          160 t
ORIGIN      
        1 gagtgaatat gagcaagctt cgtgacttta atcctatgct tgagaagtat tttaaaagcc
       61 agtattacag cttaagcagt aaatccaaga cacttggaaa cgaagctttt aatcgtgagt
      121 tagcaaaatt aaatcaatct cttcgtgaaa cactttctaa gtttaataag accaccagca
      181 aaaataatta cagtgaaaat gcttcaaata gtctggatgt attagctaca agtgcaatca
      241 aagcaggtac agccatttat ggttttcaga cagccttaga agcttatagg aaggttatgg
      301 agattggtct gaaaaaagaa gcatcacaac gagcggcaca attcgtttta ggtgatgaag
      361 gtgctaaacg agctacagaa tttgttaaga atcttgctga tagtagtggt gtagaccaga
      421 ttgaaacatt tagcagtttt gctaagtttt ccgctggtgc tggtgatatg aatgcagacc
      481 agaaagaatc cttattttct aatgtgattg gtacatcccg actaatgggt ttaagcactg
      541 atgagattaa cggga
//
LOCUS       DADLTF010000419          546 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.425, whole genome shotgun sequence.
ACCESSION   DADLTF010000419 DADLTF010000000
VERSION     DADLTF010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.425"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..171
                     /locus_tag="JN749_004472"
     CDS             <1..171
                     /locus_tag="JN749_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HAY7370593.1"
                     /translation="LFESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAET
                     IAHHQVDIDLED"
     gene            231..>546
                     /locus_tag="JN749_004473"
     CDS             231..>546
                     /locus_tag="JN749_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAY7370594.1"
                     /translation="MGFAGDGKTYTASELAIGLVMLMRQRGIEAGNRPVMFLDTETGS
                     DWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMIIDSISHFWTGLCDEYARR
                     RNR"
BASE COUNT          154 a          128 c          135 g          129 t
ORIGIN      
        1 ctgtttgaat cgctctgcat cgaccatttg caacgccacg gtgccaataa acaggcaatc
       61 agccacgcat ttgatgatga tgttgagttt caggagcgca tggcagaaca catccggtac
      121 atggctgaaa ccattgccca ccaccaggtt gatattgatt tggaggacta aaatctatgg
      181 cgctttttca acgagcaata aacacacagg cctatctaaa agcaggaatt atggggtttg
      241 ctggcgacgg caaaacctac acagcaagcg aactggctat tggtctggta atgcttatgc
      301 ggcaacgggg aattgaagct ggtaaccgcc cggtaatgtt tctggacaca gaaacaggct
      361 ctgactgggt taaaccccgc tttgatgcgg aaaatatcga attatttacg gcaaaaaccc
      421 gctcgtttgt tgatctgctt gaagccatta atgaagcgga aagcagcggt tccgtaatga
      481 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
      541 accgta
//
LOCUS       DADLTF010000420          541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.426, whole genome shotgun sequence.
ACCESSION   DADLTF010000420 DADLTF010000000
VERSION     DADLTF010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.426"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>541
                     /gene="fimC"
                     /locus_tag="JN749_004474"
     CDS             <1..>541
                     /gene="fimC"
                     /locus_tag="JN749_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HAY7370595.1"
                     /translation="KSQEITFCLLAGILMFMAMMVAGRAEAGVALGATRVIYPAGQKQ
                     VQLAVTNNDENSTYLIQSWEENADGVKDGRFIVTPPLFAMKGKKENTLRILDATNNQL
                     PQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPPDQAAEKLRF
                     RRSANSLTLINPTPYYLTVT"
BASE COUNT          166 a          115 c          127 g          133 t
ORIGIN      
        1 aaatcgcagg aaataacatt ctgcttgctg gcaggtatcc tgatgttcat ggcaatgatg
       61 gttgccggac gcgctgaagc aggagtggcc ttaggtgcga ctcgcgtaat ttatccggca
      121 gggcaaaaac aagtgcaact tgccgtgaca aataatgatg aaaatagtac ctatttaatt
      181 caatcatggg aggaaaatgc cgatggtgta aaggatggtc gttttatcgt gacgcctcct
      241 ctgtttgcga tgaagggaaa aaaagagaat accttacgta ttcttgatgc aacaaataac
      301 caattgccac aggaccggga aagtttattc tggatgaacg ttaaagcgat tccgtcaatg
      361 gataaatcaa aattgactga gaatacgcta cagctcgcaa ttatcagccg cattaaactg
      421 tactatcgcc cggctaaatt agcgttgcca cccgatcagg ccgcagaaaa attaagattt
      481 cgtcgtagcg cgaattctct gacgctgatt aacccgacac cctattacct gacggtaaca
      541 g
//
LOCUS       DADLTF010000421          541 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.427, whole genome shotgun sequence.
ACCESSION   DADLTF010000421 DADLTF010000000
VERSION     DADLTF010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.427"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..158
                     /locus_tag="JN749_004475"
     CDS             <1..158
                     /locus_tag="JN749_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HAY7370596.1"
                     /translation="ATNVIAPLQDAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPE
                     WPMHPNK"
BASE COUNT          168 a           99 c          112 g          162 t
ORIGIN      
        1 aggcaacgaa tgtaatagct ccgcttcagg atgcggttga tttaggtatg gcaacagatg
       61 atgaaacgaa actgttactg gcatggaaaa aatatcgggt gctgcttatg cgtgttaatg
      121 tagtaaaacc cgagtggcct atgcatccaa ataaatagac ttgttaatct aggataattg
      181 ttggaataat actcattttt cagtaggttc aggtagcctg ggtataatgc tcacttctac
      241 aatgatttca acttatttta tttaattgat attacgaaac ttaactcata cataaacaat
      301 atgactcaat actgtcctaa atagctgcgc agaaaagtgc tccactttcg ctcagccctt
      361 gctgggcgaa gcatcaggaa ggcgttctca gctaggttgt gttatatctg gcttgagttt
      421 tgttgcccat acgcaacaca ttacccatct gtaaacgaca agtgtgacag aattaggtgc
      481 agctatttag actatatcga cgcagtaacg ataatagata tcgaaactgt actagatatt
      541 a
//
LOCUS       DADLTF010000422          538 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.428, whole genome shotgun sequence.
ACCESSION   DADLTF010000422 DADLTF010000000
VERSION     DADLTF010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.428"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            30..538
                     /locus_tag="JN749_004476"
                     /pseudo
     CDS             30..538
                     /locus_tag="JN749_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          139 a          134 c          154 g          111 t
ORIGIN      
        1 ctaaacttcc tgagaaagag gtaaacagga tgactaaaaa tactcgtttt tcccccgaag
       61 tccgtcaacg ggcagtccgt atggttctgg aaagtcaggg cgaatatgac tcacaatggg
      121 cgacaatttg ttccattgct ccaaagattg gctgtacgcc ggagactctg cgtgtctggg
      181 ttcgccagca tgagcgggat accgggggcg gtgatggagg gctcaccacc gctgaacgtc
      241 agcgtctgaa agagctggag cgtgaaaatc gtgaactgcg ccgcagtaac gatatccttc
      301 gccaggcttc cgcttatttt gcgaaggcgg agttcgaccg cctctggaaa aaatgatgcc
      361 actgctggat aagctgcgtg agcagtacgg ggtcggaccg ctatgcagcg aactgcatat
      421 tgccccgtca acgtattacc actgtcagca acagcgacat catccggata aacgcagtgc
      481 ccgtgcgcag cgcgatgact ggctgaagaa agagatacag cgcgtatacg atgaaaat
//
LOCUS       DADLTF010000423          532 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.429, whole genome shotgun sequence.
ACCESSION   DADLTF010000423 DADLTF010000000
VERSION     DADLTF010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.429"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..276
                     /locus_tag="JN749_004477"
     CDS             <1..276
                     /locus_tag="JN749_004477"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370597.1"
                     /translation="KKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSK
                     QQNQQTSSERELEMSTEIARLKRQLAERDEELAILQKAATYFAKRLK"
     gene            273..>532
                     /locus_tag="JN749_004478"
     CDS             273..>532
                     /locus_tag="JN749_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370598.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQ
                     FRQHCDSVVLAAFTRSKQRYGAPRLTDELRAQGYPFNVKTVAA"
BASE COUNT          138 a          148 c          141 g          105 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      481 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gc
//
LOCUS       DADLTF010000424          532 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.430, whole genome shotgun sequence.
ACCESSION   DADLTF010000424 DADLTF010000000
VERSION     DADLTF010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.430"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>532
                     /locus_tag="JN749_004479"
     CDS             <1..>532
                     /locus_tag="JN749_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HAY7370599.1"
                     /translation="YLERQFAVTEPNQVWCGDVTYIWTGKRWAYLAVVLDLFARKPVG
                     WAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMS
                     RRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGL
                     PPNESENRYWKNSNSVA"
BASE COUNT          136 a          129 c          161 g          106 t
ORIGIN      
        1 actaccttga aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga
       61 cctatatctg gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa
      121 gaaaaccagt gggctgggcc atgtcgttct cgccggacag caggctcacc atgaaagcgc
      181 tggaaatggc atgggaaacc cgtggtaagc ccggcggggt gatgttccac agcgatcagg
      241 gcagtcatta tacgagcagg cagttccggc agttattgtg gcgataccag atcagacaga
      301 gtatgagccg gcgcggaaac tgctgggata acagcccaat ggaacgcttc ttcaggagtc
      361 tgaagaacga atggatgccg gtggtgggtt acgtaagctt cagcgaggca gctcacgcca
      421 taacggacta tatcgttgga tattacagcg cactaagacc gcacgaatat aacggtgggt
      481 tacccccaaa cgaatcggaa aatcgatact ggaaaaactc taactcggtg gc
//
LOCUS       DADLTF010000425          519 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.431, whole genome shotgun sequence.
ACCESSION   DADLTF010000425 DADLTF010000000
VERSION     DADLTF010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.431"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(86..364)
                     /locus_tag="JN749_004480"
     CDS             complement(86..364)
                     /locus_tag="JN749_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370600.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDSCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT          122 a          128 c          155 g          114 t
ORIGIN      
        1 gaataccggc atggattcac ggcctggctt aaggaccacc agcccgagtt ccggaaccag
       61 aacaggtcga agtaataccc gcacattacc tccagatccg ttgctggaat gtgcaggacg
      121 gacgcggtgg ccgttcggaa taagggagcc tgacgaagat tatccagtga cgataatcga
      181 gactgagggc tttcttaatc tcgtatccgt gtctgcggta gcgctgaatc agccattcag
      241 cctgttcttc ggtgcaggag tcgtgctgat accagtcaga tttgaatgca tgagaacgcc
      301 gcccgtgcct gctggcaggg gcggcagagt tatccgaatt gtaaaatttg gtatcgtgcg
      361 ccatctgttt tctctgctgg cgcagcaggt gccagttgtt caggctgacg gatggattgt
      421 aaaccagaac gaccagaaaa aacaaaaccc gccgaagcgg gttaagtgcg ggtgcgttga
      481 ggatgcctga cacatcagcg gtggcgaggg atttctccc
//
LOCUS       DADLTF010000426          512 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.432, whole genome shotgun sequence.
ACCESSION   DADLTF010000426 DADLTF010000000
VERSION     DADLTF010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.432"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>512
                     /locus_tag="JN749_004481"
     CDS             <1..>512
                     /locus_tag="JN749_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAY7370601.1"
                     /translation="YLEPETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQ
                     NWKPATLSENRASHSTLIEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSM
                     PLSDLLNRESKTDCLDVVTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKA
                     SDARDAFSPE"
BASE COUNT          133 a          138 c          127 g          114 t
ORIGIN      
        1 taccttgagc ctgagactgg cagtctaaga ccgtccgtct ccctatctgt attggcggaa
       61 cctgaagtcc ctgaacctaa agcactgacc ctcgcgtctc tcattgagca gtacgagcag
      121 gagaacgccc agaactggaa gccagcgacc ctaagcgaaa accgagcgtc acactctacg
      181 ctaatcgaga tattcgacta tctggatatt caggatgtcg gcaaggcgac ccgtgcagat
      241 atgctcagag ttcgtgaagt cctccagcag ctaccgaaga accgcaagca acgctttaag
      301 tctatgccgc tgtctgactt gctgaaccgg gagtctaaga ctgactgtct ggacgtcgtt
      361 accattaaca ataaatatct tatcaagatg gctgctgtgt ttaagtgggc ggtacgcaac
      421 gacttgatag ctaagaacct gaccgaaggt cttgagctga aagtccctca gcgtaaagcc
      481 tccgacgctc gtgatgcgtt cagtcctgag ca
//
LOCUS       DADLTF010000427          509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.433, whole genome shotgun sequence.
ACCESSION   DADLTF010000427 DADLTF010000000
VERSION     DADLTF010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.433"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            118..>509
                     /locus_tag="JN749_004482"
     CDS             118..>509
                     /locus_tag="JN749_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370602.1"
                     /translation="MCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIRTRET
                     YGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQ
                     TFAPTAPNQVWVADLTYVATQEGWLYLAG"
BASE COUNT          150 a          120 c          137 g          102 t
ORIGIN      
        1 aaatgaagct cgccttgagc gagatatatt aaaaaaagca acagcgtatt ttgcacagga
       61 gtcgctgaaa aatacgcgtt aatcgaacaa tggcgacaac aatttcccat tgaagcgatg
      121 tgtcaggtat ttggtgtatc caggagcggt tattacaact gggtacagca tgaaccctca
      181 gacagaaaac aaagtgatga gcggctaaaa ctggagatta aggtggcaca tatccgcact
      241 cgcgaaacat atggaacccg gcggctccag acggagctgg cagagaatgg catcatcgtt
      301 ggtcgtgacc gactggcacg tcttcgtaag gagctgaggc tacgctgtaa gcagaaacgc
      361 aagttcagag cgactacgaa cccgaaccac aatctgccag ttgcgccaaa tctgctgaac
      421 cagacgttcg ctcctacagc accaaatcag gtctgggtgg cggacctgac gtatgttgcc
      481 acacaggagg gatggttgta cctcgctgg
//
LOCUS       DADLTF010000428          509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.434, whole genome shotgun sequence.
ACCESSION   DADLTF010000428 DADLTF010000000
VERSION     DADLTF010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.434"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..209
                     /locus_tag="JN749_004483"
     CDS             <1..209
                     /locus_tag="JN749_004483"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370603.1"
                     /translation="VAAAARELSLYESQLYAWRSKLQQQMTSSERESELAAENARLKR
                     QLAEQAEELAILQKAATYFAKRLK"
     gene            206..>509
                     /locus_tag="JN749_004484"
     CDS             206..>509
                     /locus_tag="JN749_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370604.1"
                     /translation="MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTGISPRQQ
                     FRQHCDSVVLAAAFTRSKQRYGAPRLTDELRAQGYHFNVKTVAASLRRQGLRAKASR"
BASE COUNT          117 a          143 c          153 g           96 t
ORIGIN      
        1 gtgttgctgc cgcagcccgt gaactcagcc tgtatgaatc tcagctctat gcgtggcgca
       61 gcaaactgca gcaacagatg acctcgtctg agcgtgaaag tgaactggcc gctgaaaatg
      121 cccgcctgaa gcggcaactg gcggagcagg cagaggaact ggctatcctc caaaaggccg
      181 cgacatactt tgcgaagcgc ctgaaatgaa gtatgtcttt attgaaaaac atcaggctga
      241 gttcagcatc aaggcaatgt gccgcgtgct ccgggtggcc cgcagcggct ggtatacgtg
      301 gtgtcagcgg cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt
      361 tgtcctcgct gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga
      421 tgaactgcgt gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg
      481 tcaggggctg agggcaaagg cctcccgga
//
LOCUS       DADLTF010000429          509 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.435, whole genome shotgun sequence.
ACCESSION   DADLTF010000429 DADLTF010000000
VERSION     DADLTF010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.435"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..>509)
                     /locus_tag="JN749_004485"
     CDS             complement(<1..>509)
                     /locus_tag="JN749_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAY7370605.1"
                     /translation="GLMGVGSAISIVSRIMSMTPIGMIATAIAMAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINHWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDPAQSNNASDATGPNPFMIN
                     KASAPKVDG"
BASE COUNT          127 a          156 c          112 g          114 t
ORIGIN      
        1 tcaccatcaa cttttggcgc agaagctttg ttaatcatga agggattcgg gcctgtggca
       61 tcggaggcgt tgtttgactg agccggatcc accgccggat aaggtgtgta tccccgtgcc
      121 ggtattcccg tcccataagc atcataagca cccgcgcccc actgcgcaga gttaatggca
      181 tcgaccgtgt ctccggaact gtcggtaaac cactcaataa ttggcttcag cttgtcccac
      241 atatcctgaa accacttaac aaccggcccc cagtgattga tcaccatccc cagcggcgac
      301 caggcaaaaa ctttcttaag gagttcccag ccagcctcaa aataaggacc aatggtttcc
      361 cagagcttct tgaaataagg tccgacaaca tcccagttag tgataattaa tcccgcagcc
      421 atggctatcg ccgtcgcaat catgccaatc ggcgtcatcg acatgatcct gctgacaatg
      481 ctgatggcac tgcccacgcc catcaatcc
//
LOCUS       DADLTF010000430          485 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.436, whole genome shotgun sequence.
ACCESSION   DADLTF010000430 DADLTF010000000
VERSION     DADLTF010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.436"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            114..485
                     /locus_tag="JN749_004486"
                     /pseudo
     CDS             114..485
                     /locus_tag="JN749_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          132 a          127 c          141 g           85 t
ORIGIN      
        1 atgcacaacg ttttgcggag aaagcccgac aggatgcaac cgtcacagct gaggacagaa
       61 aggccactgc ggaagatgtg acaagcacag gagcaaatgc agccgcagcc ggacagagcg
      121 cacaggatgc cgcaggttat gcccgcgcag cagaacaggc caaaaatgac attgatgctg
      181 cgctgaccgg caccctgaaa acggctaacc atctgtctga aatcgcagca gcaggcgaaa
      241 aggcacaaca gaagtcccgg gataatctgg ggctgaaaag tgcggccacg atggaagcac
      301 agagcgacat ttacgaccgg acaaaaggcc gtctggcgat acccggcgca ttcggctttg
      361 ggtgtgcttt tctgcctgaa gatgttatcc gttttgacac taagagtgat ttcctggcct
      421 gggtaaggaa tgcgctgcca ggtgaatatt ccgttgctgg cccttacggc atcatcatac
      481 ccgac
//
LOCUS       DADLTF010000431          479 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.437, whole genome shotgun sequence.
ACCESSION   DADLTF010000431 DADLTF010000000
VERSION     DADLTF010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.437"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..250)
                     /locus_tag="JN749_004487"
     CDS             complement(<1..250)
                     /locus_tag="JN749_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370606.1"
                     /translation="MDITVCTYKPGGIVEKIKKLFSSKYAVIRRDDLSVIVEMDYFPE
                     TPKSIMYRNGRKAIFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          136 a           98 c           89 g          156 t
ORIGIN      
        1 aacaggatgc tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta
       61 ccctcatcgg taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt
      121 cagggaagta atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt
      181 tgctactaaa tagtttcttt attttctcca cgatgcctcc aggtttataa gtacaaacgg
      241 ttatatccac atagagacaa aaatattaat ctgaaaaata tttatttcac gccgtatatt
      301 tgattgttta atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat
      361 atggttattt ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta
      421 gtgcgggtgc gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgcc
//
LOCUS       DADLTF010000432          479 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.438, whole genome shotgun sequence.
ACCESSION   DADLTF010000432 DADLTF010000000
VERSION     DADLTF010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.438"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            28..>479
                     /locus_tag="JN749_004488"
     CDS             28..>479
                     /locus_tag="JN749_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HAY7370607.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFK"
BASE COUNT          154 a           97 c          104 g          124 t
ORIGIN      
        1 tcttgattaa ataaaatgga gttttacatg aaggctttca ataagctgtt ttccctcgtt
       61 gttgcatctg ttctggtttt ctctcttgct ggctgcggtg acaaagaaga atcgaagaaa
      121 ttcagcgcca atctgaacgg cactgaaatt gccattacct atgtctacaa aggtgacaag
      181 gtgcttaagc aatcttctga aaccaaaatt caatttgcct ccattggtgc aaccaccaaa
      241 gaagatgctg ccaagacact tgagccgtta agcgccaaat acaaaaacat cgcgggtgtt
      301 gaagaaaaat caacctatac cgatacctac gcgcaggaaa acgtgactat cgatatggaa
      361 aaagtggatt ttaaagccct gcagggtatt tcaggaatca acgtttctgc tgaagatgcc
      421 aaaaaaggta tcactatggc gcaaatggaa ctggtgatga aagccgctgg ttttaaaga
//
LOCUS       DADLTF010000433          475 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.439, whole genome shotgun sequence.
ACCESSION   DADLTF010000433 DADLTF010000000
VERSION     DADLTF010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.439"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>475
                     /locus_tag="JN749_004489"
     CDS             <1..>475
                     /locus_tag="JN749_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7370608.1"
                     /translation="ERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFG
                     NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
                     YISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCL"
BASE COUNT          118 a          111 c          151 g           95 t
ORIGIN      
        1 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
       61 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      121 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      181 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      241 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      301 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      361 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcgctgggt tatcgctcgc
      421 cacgggaata tctgcggcag cgggcttgta atgggttaag tgataacaga tgtct
//
LOCUS       DADLTF010000434          473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.440, whole genome shotgun sequence.
ACCESSION   DADLTF010000434 DADLTF010000000
VERSION     DADLTF010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.440"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(88..237)
                     /gene="hokB"
                     /locus_tag="JN749_004490"
     CDS             complement(88..237)
                     /gene="hokB"
                     /locus_tag="JN749_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HAY7370609.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(337..>473)
                     /locus_tag="JN749_004491"
     CDS             complement(337..>473)
                     /locus_tag="JN749_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370610.1"
                     /translation="FSNISELKDAVTEYIEYYNSRRISLKLKGLTPNEYRNQTYMPRV
                     "
BASE COUNT          120 a          110 c          115 g          128 t
ORIGIN      
        1 aacatgcact aactgaagtg taaaaggggt gccatgagaa aatgaatctg ctagtcagat
       61 gcgcggggaa aatccccgcg cttgccctta cctggacgtg caggccatga gcgcagcaac
      121 ctccttatca ccgtcccgga accgcagttc gtagagcgtt tgtcgggtca ggagtgtgaa
      181 tgccagaatc gtaatgcaga taatgagcag acacaccacc agagggttgc gcttcatagc
      241 ctttctcctt gccggatggc gggtaagagg ctaacatctg aattgcttgg ttcattcgtt
      301 ggcctcggtt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca tataggtctg
      361 attccgatat tcatttggag tcagaccttt taatttcagg ctaattcttc tgctgttgta
      421 gtattcaata tattccgtaa cagcatcctt cagttcgctt atattactga act
//
LOCUS       DADLTF010000435          473 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.441, whole genome shotgun sequence.
ACCESSION   DADLTF010000435 DADLTF010000000
VERSION     DADLTF010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.441"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..410)
                     /locus_tag="JN749_004492"
     CDS             complement(<1..410)
                     /locus_tag="JN749_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HAY7370611.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEH"
BASE COUNT          110 a          117 c           94 g          152 t
ORIGIN      
        1 tcatgttcga acaccactcc aacatctgtt tgaacacctg ttaaatttct ctcttgtttt
       61 gtttgaacat ctgcttcctt tctgcttctt ctggcctgaa cagatgcttt tcctgcggct
      121 gattttttgg ttaatttttc tctgacagat gccagatctt cctcaatccg aagatgcatc
      181 cattcgtcgc cgttatcaca aaaaaactcc cgcaaggatg gttcaacatc aacccatcgc
      241 tcgttagtca gacgggcaat ttttgccaac ctgtttttgg gtattggctt tcctgtttgc
      301 cagtaattga acatcagcaa caaatacgcg ccgtgctcct ctgctgacaa atgcatggtg
      361 tcagccagat aatcagctat gtacagttgc atgtatggta atgcggccat aattgccccg
      421 tatgatgcta cccggttgct tagaataagt gaggtagcct gagtttaacg gac
//
LOCUS       DADLTF010000436          462 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.442, whole genome shotgun sequence.
ACCESSION   DADLTF010000436 DADLTF010000000
VERSION     DADLTF010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.442"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..183
                     /locus_tag="JN749_004493"
     CDS             <1..183
                     /locus_tag="JN749_004493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370612.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..>462
                     /locus_tag="JN749_004494"
     CDS             278..>462
                     /locus_tag="JN749_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HAY7370613.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRG"
BASE COUNT          137 a           91 c          136 g           98 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aa
//
LOCUS       DADLTF010000437          442 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.443, whole genome shotgun sequence.
ACCESSION   DADLTF010000437 DADLTF010000000
VERSION     DADLTF010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.443"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            211..>442
                     /locus_tag="JN749_004495"
     CDS             211..>442
                     /locus_tag="JN749_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HAY7370614.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          139 a          106 c           63 g          134 t
ORIGIN      
        1 tcgaacctcg cttaccaacg cgttgtcagt catctccccc ctccctggtg catcgttata
       61 tccgtgatga gcgatttttg atattaatat ctttcaccat ccgctatttt caccacttca
      121 gaaaacaaac ttatttttat taaaatcaat tctttttctt tgaacattca actgcgatat
      181 ttgtaaaaac attaaacagg caacaataaa atgacattac caacaactat ttattcattc
      241 cccgcatatt taccccgttt ttcgtccacc gacaaaccag caaaattaaa atttcatcaa
      301 tacgcacgcg ctacattact gtcaaatcgt ggaagagatc ataattgcga tggcagacgt
      361 actgttgaga tccacaaact agatctctct gactggcaag cattcaataa gttagcaacg
      421 aagtgtaatg cttacgatgg ag
//
LOCUS       DADLTF010000438          436 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.444, whole genome shotgun sequence.
ACCESSION   DADLTF010000438 DADLTF010000000
VERSION     DADLTF010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.444"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>436
                     /locus_tag="JN749_004496"
     CDS             <1..>436
                     /locus_tag="JN749_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7370615.1"
                     /translation="PLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKT
                     AQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQ
                     MELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY"
BASE COUNT           99 a          139 c          116 g           82 t
ORIGIN      
        1 cctcttcacc agattgtgaa tcgcctggac atcatgctgc cgggcaatcg ccccttcgtt
       61 gcccccagtg ccgttattca ggcccgccag cgcctgggaa gtgaggctgt ccgccgcgtg
      121 ttcacgaaaa cagcgcagct ctggcataac gccacgccgc atccgcactg gtgcggcctg
      181 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
      241 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      301 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      361 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      421 atggataaag gttatt
//
LOCUS       DADLTF010000439          429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.445, whole genome shotgun sequence.
ACCESSION   DADLTF010000439 DADLTF010000000
VERSION     DADLTF010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.445"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            128..>429
                     /locus_tag="JN749_004497"
     CDS             128..>429
                     /locus_tag="JN749_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HAY7370616.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSSSDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGIQTLRQRQQRLDAKVNLAGGINED"
BASE COUNT           93 a          114 c          131 g           91 t
ORIGIN      
        1 aactgaaggt atcgcgtatc agtgaggcga tttccggtgc ggacggggaa tacagccatc
       61 agttgcttgc accggtggac aatatctcca ttgcgaaaaa cgaactggcg gtactgggga
      121 cgatttcatg gacgtgacaa acgatgatta catccgcctg ttatcggcac tgttgccgcc
      181 cggtccggcg tggtcatcta gcgatccggc gattgccggt gcggcaccgt cattaacccg
      241 tgttcatcag cgtgcggatg ccctgatgcg ggagctggat ccgcgcacca ccactgaact
      301 gataaaccgc tgggagcgtc tgtgcggtct gccggatgaa tgtattcccg caggaataca
      361 gacccttcgc cagcgtcagc aacgactgga tgcgaaggtt aacctggcgg gcggcatcaa
      421 tgaggattt
//
LOCUS       DADLTF010000440          429 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.446, whole genome shotgun sequence.
ACCESSION   DADLTF010000440 DADLTF010000000
VERSION     DADLTF010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.446"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            108..>429
                     /locus_tag="JN749_004498"
     CDS             108..>429
                     /locus_tag="JN749_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370617.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPCGVNIRGVGKASQLRAKSGLTGS
                     VLRLS"
BASE COUNT          121 a           87 c          105 g          116 t
ORIGIN      
        1 attttccgaa tttgctgaag tatgaccctg atcagttcgg tccggattta attgagcaac
       61 tagctcaatc cggtaagtat tcgcaggata acaccaaagg cgatgccatg attggcgtca
      121 agcagccttt accaaaagca gttttaagaa ctcagcatga caaaaataaa gaagcaataa
      181 gtatcctgga ttttggtgtt attgatgatg gtgtgacaga taattaccag gcaatacaaa
      241 atgcaataga tgccgttgct tcactaccct ccggcgggga gctgtttatc cctgcgagca
      301 accaagcggt ggggtatatt gttggatcca ctttgcttat tccttgcggt gttaacatca
      361 gaggggttgg taaggcatcg caactccgag caaaaagcgg acttacagga tctgtgttaa
      421 ggctgtctt
//
LOCUS       DADLTF010000441          408 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.447, whole genome shotgun sequence.
ACCESSION   DADLTF010000441 DADLTF010000000
VERSION     DADLTF010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.447"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            170..361
                     /locus_tag="JN749_004499"
                     /pseudo
     CDS             170..361
                     /locus_tag="JN749_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900875.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
BASE COUNT          114 a          100 c          101 g           93 t
ORIGIN      
        1 attacgcaag gctgttgcag ttgctattgg tggtggggct attgccatag cgtctgtgct
       61 catcactggc ccaggtggta acgatggtct ggaaggtgtc agctacatac catacaaaga
      121 tatcgttggc gtatggactg tatgccacgg acacaccgga aaagacatca tgctcggtaa
      181 aacgtatacc gaagcagaat gcaaagccct cctgaataaa gaccttgcca ctgtcgccag
      241 acaaattaac ccgtacatca aagtcgatat accggaaaca acgcgcggcg ctctttactc
      301 gttcgtttac aacgtgggcg ctggcaattt cagaacatcg atgcttcttc gcaaaataaa
      361 ctagggcgat atcaaaggcg catgtgacca gctacgtcgc tggacata
//
LOCUS       DADLTF010000442          403 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.448, whole genome shotgun sequence.
ACCESSION   DADLTF010000442 DADLTF010000000
VERSION     DADLTF010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.448"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            189..>403
                     /locus_tag="JN749_004500"
     CDS             189..>403
                     /locus_tag="JN749_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAY7370618.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSR"
BASE COUNT          125 a           86 c           86 g          106 t
ORIGIN      
        1 agtctgcatt aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta
       61 tggctgcatt tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg
      121 ttgctttctc agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg
      181 tactaaccat ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc
      241 caaaaacaat ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca
      301 ttgaagctgc acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt
      361 ttactaacgg ctatgtggca cacggtgttt ccggcccctc ccg
//
LOCUS       DADLTF010000443          401 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.449, whole genome shotgun sequence.
ACCESSION   DADLTF010000443 DADLTF010000000
VERSION     DADLTF010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.449"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..219
                     /locus_tag="JN749_004501"
     CDS             1..219
                     /locus_tag="JN749_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAY7370619.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVKNANVPKGSFYYYLKSKEDYAVNDG"
     gene            complement(270..>401)
                     /locus_tag="JN749_004502"
     CDS             complement(270..>401)
                     /locus_tag="JN749_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370620.1"
                     /translation="DAALTSAMLDRILHHSHIVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          105 a           90 c           96 g          110 t
ORIGIN      
        1 atgacaggcg aaaatcgtgg ccggggaagg ccgcgaaaga cagaaagtac ctatgcagat
       61 actcgtaatg atctgatccg cagtggcctt gaactgctga cgcagaatgg ttttcttgcg
      121 acgggagtcg atgccattgt taaaaatgcc aatgtcccta aaggctcatt ttattactac
      181 cttaagagta aagaggatta tgctgtcaac gacggatgaa aagtgatcca cttatatctc
      241 caccaacggc ccaatattga tccaccgttt tactcaggat tagcttctgc tataaccccg
      301 gcctttcgtt tctgtctgag tcgatagctt tctcctttga tttgaacgat atgtgagtgg
      361 tgtaagatac ggtccagcat cgctgaggtc agtgctgcat c
//
LOCUS       DADLTF010000444          385 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.450, whole genome shotgun sequence.
ACCESSION   DADLTF010000444 DADLTF010000000
VERSION     DADLTF010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.450"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            17..340
                     /locus_tag="JN749_004503"
                     /pseudo
     CDS             17..340
                     /locus_tag="JN749_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          106 a           91 c          112 g           76 t
ORIGIN      
        1 agtcattgga gaacagatga ttgatgtctt agggccggag aaacgcagac ggcgtaccac
       61 acaggaaaag atcgcaattg ttcagcagag ctttgaaccg gggatgacgg tctccctcgt
      121 tgcccggcaa catggtgtag cagccagcca gttatttctc tggcgtaagc aataccagga
      181 aggaagtctt actgctgtgg ccgccggaga acaggttgtt cctgcctctg aacttgctgc
      241 cgccatgaag cagattaaag aactccagcg cctgctcggc aagaaaacga tggaaaatga
      301 actcctcaaa gaagccgttg aatatggacg ggcaaaaaag tggatagcgc acgcgccctt
      361 attgcccggg gatggggagt aagct
//
LOCUS       DADLTF010000445          375 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.451, whole genome shotgun sequence.
ACCESSION   DADLTF010000445 DADLTF010000000
VERSION     DADLTF010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.451"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>375
                     /locus_tag="JN749_004504"
     CDS             <1..>375
                     /locus_tag="JN749_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370621.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTT"
BASE COUNT           86 a           96 c          111 g           82 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actac
//
LOCUS       DADLTF010000446          374 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.452, whole genome shotgun sequence.
ACCESSION   DADLTF010000446 DADLTF010000000
VERSION     DADLTF010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.452"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            1..374
                     /locus_tag="JN749_004505"
                     /pseudo
     CDS             1..374
                     /locus_tag="JN749_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558297.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           94 a           98 c           98 g           84 t
ORIGIN      
        1 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
       61 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
      121 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
      181 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
      241 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
      301 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
      361 gtgcggagta aaca
//
LOCUS       DADLTF010000447          362 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.453, whole genome shotgun sequence.
ACCESSION   DADLTF010000447 DADLTF010000000
VERSION     DADLTF010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.453"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>362
                     /locus_tag="JN749_004506"
     CDS             <1..>362
                     /locus_tag="JN749_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370622.1"
                     /translation="GYRSPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHE
                     GWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPEKQVLVHSDQGSQYT
                     SHEWQSFLKSHGLEGSMS"
BASE COUNT           88 a           95 c          103 g           76 t
ORIGIN      
        1 cggataccga agcccgcggg cacgtaaagg cgaggccagt atcgtgtcac ccaacaggct
       61 ccagcgacag ttcaatccgg atgctcctga tgagcgttgg gtaacggaca taacctacat
      121 caggacccac gaaggctggc tgtatcttgc cgttgttgtt gatctgttct cacgcaaaat
      181 tatcggctgg tccatgcaat cccggatgac aaaggacatt gtcctgaacg cactgctgat
      241 ggctgtatgg cggcgtaatc ccgaaaaaca ggtgctggtt cattcggatc agggcagtca
      301 gtacacaagc catgagtggc agtcgttcct gaaatcacac ggcctggagg gtagcatgag
      361 cc
//
LOCUS       DADLTF010000448          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.454, whole genome shotgun sequence.
ACCESSION   DADLTF010000448 DADLTF010000000
VERSION     DADLTF010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.454"
                     /strain="EG0002"
                     /db_xref="taxon:623"
BASE COUNT          107 a           92 c           69 g           83 t
ORIGIN      
        1 tccctcgcga tattaacgta atatcttaat tgtatgcacc gccagaatgc gttcagtgcg
       61 atcacggcag cagacaggta aaaatagcaa caaaccaccc gaaaaaccgc cgcgatcgcg
      121 tatgataaat tttaaccaca tgcatacctg tgcagcaagg cgaatcacgc acggatttcc
      181 agccagagac acctgaaacg taaccggctc atttaaaccg tctggtctgt tacctccggt
      241 tccacaaaaa taatgtccat cattttgaag tggacactat cgtatggaat accggacctg
      301 aatcactgaa gctttacgcc ttcatttcga agaacattta ccacgggttg t
//
LOCUS       DADLTF010000449          351 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.455, whole genome shotgun sequence.
ACCESSION   DADLTF010000449 DADLTF010000000
VERSION     DADLTF010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.455"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..208)
                     /locus_tag="JN749_004507"
     CDS             complement(<1..208)
                     /locus_tag="JN749_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HAY7370623.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="JN749_004508"
     CDS             complement(205..>351)
                     /locus_tag="JN749_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HAY7370624.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DADLTF010000450          346 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.456, whole genome shotgun sequence.
ACCESSION   DADLTF010000450 DADLTF010000000
VERSION     DADLTF010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.456"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            43..>346
                     /locus_tag="JN749_004509"
     CDS             43..>346
                     /locus_tag="JN749_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7370625.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRT"
BASE COUNT           74 a           91 c          100 g           81 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactg
//
LOCUS       DADLTF010000451          344 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.457, whole genome shotgun sequence.
ACCESSION   DADLTF010000451 DADLTF010000000
VERSION     DADLTF010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.457"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>344
                     /locus_tag="JN749_004510"
     CDS             <1..>344
                     /locus_tag="JN749_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370626.1"
                     /translation="VRPVCSELHIAPSTYYHCQQQRHHPDKRSARAQHDDWLKKEIQR
                     VYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSRKTVA
                     TGDRVNRQFVAER"
BASE COUNT           87 a           85 c          104 g           68 t
ORIGIN      
        1 gtcagaccgg tatgcagcga actgcatatt gccccgtcaa cgtattacca ttgtcagcaa
       61 cagcgacatc atccggataa acgcagtgcc cgtgcgcagc acgatgactg gctgaagaaa
      121 gagatacagc gcgtatacga tgaaaatcat caggtgtacg gtgtgcgtaa agtctggcgt
      181 cagttgttac gggaaggaat cagggtggcc agatgtacag tggcgcgcct catggcggtt
      241 atgggacttg ccggtgttct ccggggtaaa aaggtccgca ctaccgtcag ccggaaaacc
      301 gttgccacag gtgaccgcgt aaaccgtcag ttcgtggcag aacg
//
LOCUS       DADLTF010000452          329 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.458, whole genome shotgun sequence.
ACCESSION   DADLTF010000452 DADLTF010000000
VERSION     DADLTF010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.458"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..290
                     /locus_tag="JN749_004511"
     CDS             <1..290
                     /locus_tag="JN749_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAY7370627.1"
                     /translation="EDINKPISIAAANRFVNRIRGGMDLGYWRTHDFRRTLVTRLSEM
                     NVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELHADKLFWHIRSISD"
BASE COUNT          102 a           74 c           80 g           73 t
ORIGIN      
        1 gagaagacat aaacaaaccg atcagcatcg ctgcagccaa ccgattcgta aatagaataa
       61 ggggagggat ggatctaggt tactggcgaa cacatgattt cagaagaacg cttgttacac
      121 ggctgtccga gatgaatgtc gagcctcatg ttactgagcg aatgctcggt catgaacttg
      181 gcgggataat gtccgtatac aataaacacg actggataga ggctcagcgc aaagcgtatg
      241 agcttcacgc tgataaattg ttctggcaca tcaggagcat ttctgattaa cgccaccgtt
      301 aagaatccac ccttcaacag cttcacgaa
//
LOCUS       DADLTF010000453          327 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.459, whole genome shotgun sequence.
ACCESSION   DADLTF010000453 DADLTF010000000
VERSION     DADLTF010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.459"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>327
                     /locus_tag="JN749_004512"
     CDS             <1..>327
                     /locus_tag="JN749_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HAY7370628.1"
                     /translation="RNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKRE
                     GRCPSDVEHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKG
                     QASAFY"
BASE COUNT           93 a           85 c           83 g           66 t
ORIGIN      
        1 tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg tgaagaagtg
       61 gcagatcccg cgattcatca acacggataa agcgcccgcc tatggtcgcg cgcttgctct
      121 gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta agtaccggaa
      181 caacgtgatt gaatgcgatc atggcaaact gaaacggata atcggcgcca cgctgggatt
      241 taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc gtgcactacg
      301 caaaggccag gcctcagcat tttatta
//
LOCUS       DADLTF010000454          326 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.460, whole genome shotgun sequence.
ACCESSION   DADLTF010000454 DADLTF010000000
VERSION     DADLTF010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.460"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(1..326)
                     /locus_tag="JN749_004513"
                     /pseudo
     CDS             complement(1..326)
                     /locus_tag="JN749_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT           94 a           80 c           53 g           99 t
ORIGIN      
        1 ggctgtacgc ttccccatgt gatttgcctc gccacttcac cgggctcagg accccgtagg
       61 atcttcaccg aaaaaaatgc atcaccagtt gctttattat ttgttttagg tagacctgtt
      121 actaatttat acctactctg accaagtctg gataagaaag gcacacctgc acatttctac
      181 ttggaaatgt tttcttttac cattttgctc aaaaatttca acatccaata ttcctcgtac
      241 agaactactc aagtcctgaa tagaaaaagt tccagctggt acagtagagt catatattac
      301 tcgaccttgt tgagatacaa caacac
//
LOCUS       DADLTF010000455          324 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.461, whole genome shotgun sequence.
ACCESSION   DADLTF010000455 DADLTF010000000
VERSION     DADLTF010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.461"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>324
                     /locus_tag="JN749_004514"
     CDS             <1..>324
                     /locus_tag="JN749_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAY7370629.1"
                     /translation="YREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
                     AEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGR
                     AFPRV"
BASE COUNT           95 a           57 c          103 g           69 t
ORIGIN      
        1 gatacaggga gataaaacag acgatgcaac tgagcaggct gacgctgaga agtaaaaagc
       61 cggagcttgt ggagcaagag ctgtggggtg tcttactggc ttataatctg gtgagatatc
      121 agatgattaa aatggcggaa catctgaaag gttactggcc gaatcaactg agtttctcag
      181 aatcatgcgg aatggtgatg agaatgctga tgacattgca gggcgcttca ccgggacgta
      241 taccggagct gatgcgcgat cttgcaagta tgggacaact tgtgaaatta ccgacgagaa
      301 ggggaagggc cttcccgaga gtgg
//
LOCUS       DADLTF010000456          322 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.462, whole genome shotgun sequence.
ACCESSION   DADLTF010000456 DADLTF010000000
VERSION     DADLTF010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.462"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>322
                     /locus_tag="JN749_004515"
     CDS             <1..>322
                     /locus_tag="JN749_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370630.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRR"
BASE COUNT           72 a           74 c           83 g           93 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctgttag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gc
//
LOCUS       DADLTF010000457          309 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.463, whole genome shotgun sequence.
ACCESSION   DADLTF010000457 DADLTF010000000
VERSION     DADLTF010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.463"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            66..>309
                     /locus_tag="JN749_004516"
     CDS             66..>309
                     /locus_tag="JN749_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370631.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIATSPDLSEAAVRLEALWDHLGNNQVLSVNR"
BASE COUNT           68 a           70 c          102 g           69 t
ORIGIN      
        1 cgcctctgct ctgcagaacg cctctctgaa agtatggtcg atgaaatatt tcagaagggg
       61 gctgcatgag tacagaaatg aaaacggggc tggtgctgtc cggtggcggg gcggtgggcg
      121 cttatcaggc gggagtggtt aaggcactgg cagagtgtga tacacagatc agcatggttt
      181 caggggccag cattggcgca ttcaatggtg ccattatcgc gacctctccc gatctgtcag
      241 aagctgccgt acgcctggaa gcgctctggg atcatctggg gaataatcag gtgctgtcgg
      301 taaaccgat
//
LOCUS       DADLTF010000458          308 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.464, whole genome shotgun sequence.
ACCESSION   DADLTF010000458 DADLTF010000000
VERSION     DADLTF010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.464"
                     /strain="EG0002"
                     /db_xref="taxon:623"
BASE COUNT           92 a           71 c           68 g           77 t
ORIGIN      
        1 cgcccgaagg ttagtatttc tgcacagcaa ttttgcaaaa aaaggcgatc attcataact
       61 taaacgtctt tcagtcactc cgggatttcc catcatcgca gactgaaaga ctctgactgg
      121 agcgggcagc gggagcctac gacaaatcga agacaaccag ctaacctatt gtttcttaaa
      181 gacaggtgaa tccagttggt tgtagtgata gtcacacatt tagagataga aggtcaagac
      241 ttgaagcacg taatattccc tctaaaggaa tagaactcac ggtctgacta cccggccctt
      301 ttggtctg
//
LOCUS       DADLTF010000459          305 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.465, whole genome shotgun sequence.
ACCESSION   DADLTF010000459 DADLTF010000000
VERSION     DADLTF010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.465"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>305
                     /locus_tag="JN749_004517"
     CDS             <1..>305
                     /locus_tag="JN749_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370632.1"
                     /translation="LGEAAKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCG
                     QLNGAPNVSSTYGMRFPMTFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSP"
BASE COUNT           77 a           64 c           83 g           81 t
ORIGIN      
        1 gtttgggaga agcagctaaa aggaatgtag gtacaggggc gaatcagata cctgatatga
       61 gcctgttcgc gtcaattaat accgtaacgg ctgctgcgca aaaatttccg tctggattaa
      121 ttttacagtg cggtcagttg aatggtgccc cgaatgtatc ttcaacatac gggatgaggt
      181 tcccgatgac gttctcaaga gtcattgctg tcgtagttac attgaacgtt actggcgcgg
      241 ctgggcagcc gactgtatcg gcgacaagtg tccagaacac tggatttaat attactgtgt
      301 cgccc
//
LOCUS       DADLTF010000460          302 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.466, whole genome shotgun sequence.
ACCESSION   DADLTF010000460 DADLTF010000000
VERSION     DADLTF010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.466"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>302
                     /locus_tag="JN749_004518"
     CDS             <1..>302
                     /locus_tag="JN749_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7370633.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQEL"
BASE COUNT           59 a           92 c           88 g           63 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc tttctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ct
//
LOCUS       DADLTF010000461          295 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.467, whole genome shotgun sequence.
ACCESSION   DADLTF010000461 DADLTF010000000
VERSION     DADLTF010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.467"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..>295)
                     /locus_tag="JN749_004519"
     CDS             complement(<1..>295)
                     /locus_tag="JN749_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7370634.1"
                     /translation="MDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYNSHRTK
                     REEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITETVR"
BASE COUNT           70 a           93 c           55 g           77 t
ORIGIN      
        1 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
       61 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
      121 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
      181 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
      241 gtggcttttt caggtaagag ccaaagtaca ccgccacatg ggtggcatta tccat
//
LOCUS       DADLTF010000462          290 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.468, whole genome shotgun sequence.
ACCESSION   DADLTF010000462 DADLTF010000000
VERSION     DADLTF010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.468"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            50..>290
                     /locus_tag="JN749_004520"
     CDS             50..>290
                     /locus_tag="JN749_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004968843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370635.1"
                     /translation="MCGRAGYEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQM
                     EKQMNIETGQVWRTARILKDRSTRIDGQVFSNPTFK"
BASE COUNT           95 a           61 c           70 g           64 t
ORIGIN      
        1 gtggcgacgc tttaccgatc gatttgttaa cagtcaggcg cacattatta tgtgcgggcg
       61 tgcaggatat gagtatgact ttttcgaagg tgatgatggc aaacgccagt tggcaaaaac
      121 cggtataaaa atgaaagcag aaacagaaac cggttatgaa ccctccatcc tgatccagat
      181 ggagaagcaa atgaacatag aaaccggtca ggtgtggcgc accgccagaa tactaaaaga
      241 tcgctccacc cgtattgatg gacaggtatt ttcaaaccca acatttaaaa
//
LOCUS       DADLTF010000463          289 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.469, whole genome shotgun sequence.
ACCESSION   DADLTF010000463 DADLTF010000000
VERSION     DADLTF010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.469"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>289
                     /locus_tag="JN749_004521"
     CDS             <1..>289
                     /locus_tag="JN749_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAY7370636.1"
                     /translation="ILRRTDDWMDGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWAL
                     LRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESN"
BASE COUNT           71 a           73 c           82 g           63 t
ORIGIN      
        1 cattctcaga cgaaccgatg actggatgga tggccgccgc agtcgtcaca ctgatgatac
       61 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
      121 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      181 acgtgtttac cggatcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      241 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaat
//
LOCUS       DADLTF010000464          276 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.470, whole genome shotgun sequence.
ACCESSION   DADLTF010000464 DADLTF010000000
VERSION     DADLTF010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.470"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>276
                     /locus_tag="JN749_004522"
     CDS             <1..>276
                     /locus_tag="JN749_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HAY7370637.1"
                     /translation="QLITEVILSSHSRAAALAVDSAAAKLMQNADNISEYLSLHHQGL
                     MQSIQSLQAHITSLLEDIREIADCQEQVTADVRMAMEEIDARTRELL"
BASE COUNT           79 a           66 c           75 g           56 t
ORIGIN      
        1 atcaactgat aacggaagtc atactgagtt cacactccag agctgcggca ctggcggttg
       61 attctgccgc cgcaaaactg atgcagaatg cagacaatat cagtgaatac ctgtcgttac
      121 accatcaggg gctgatgcaa agtattcagt cactgcaggc gcatatcacc agcctgcttg
      181 aggatatccg ggaaatagca gactgtcagg agcaggtgac cgctgatgtc agaatggcca
      241 tggaggagat cgatgccaga acccgggaat tactga
//
LOCUS       DADLTF010000465          276 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.471, whole genome shotgun sequence.
ACCESSION   DADLTF010000465 DADLTF010000000
VERSION     DADLTF010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.471"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            88..>276
                     /locus_tag="JN749_004523"
     CDS             88..>276
                     /locus_tag="JN749_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAY7370638.1"
                     /translation="MYGGSPADIAAAIWRKAPPGIDMNGNTTFTVADKEYDPPYPEYV
                     ISWQTLKPVSLHVSVTLKK"
BASE COUNT           77 a           71 c           70 g           58 t
ORIGIN      
        1 gtttacgtca cccataatcc gaaaaaaaca gaacaaaaag ccggagtcag tcagtatcca
       61 ttaacacccg gttcgcttta tgttggcgtg tacggcggca gcccggcaga tatcgcggcg
      121 gccatctggc gtaaggctcc gccgggtatt gatatgaatg gcaacacaac gttcactgtt
      181 gcagataagg agtacgatcc gccgtatcct gaatacgtga tcagctggca gacactcaaa
      241 cccgtcagtc tgcatgtcag tgtgacgctg aaaaaa
//
LOCUS       DADLTF010000466          271 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.472, whole genome shotgun sequence.
ACCESSION   DADLTF010000466 DADLTF010000000
VERSION     DADLTF010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.472"
                     /strain="EG0002"
                     /db_xref="taxon:623"
BASE COUNT           76 a           54 c           60 g           81 t
ORIGIN      
        1 gcatcgggtt ggacaattta caaaagaaag ataacccata ttgtataatg cgtttaaata
       61 tgccgtctta tataaaatga tggtcagatt aatatgcgga ttatctttct acgcaaggag
      121 tatttatctc tactcccgtc aatgattgca tctcttttct ctgctaacgg tgtcgcggcg
      181 gccattgatt tatgccaggg atatgatatc aaagcgagtt gtcacgccag caggcaaagc
      241 ctttcaggca ttacgcagga ctggagtatc g
//
LOCUS       DADLTF010000467          270 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.473, whole genome shotgun sequence.
ACCESSION   DADLTF010000467 DADLTF010000000
VERSION     DADLTF010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.473"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..>270)
                     /locus_tag="JN749_004524"
     CDS             complement(<1..>270)
                     /locus_tag="JN749_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HAY7370639.1"
                     /translation="EGLALANNFIEQLPELPFSMNRAVLMNNNLTTLPESVLRLAQNA
                     FVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSATISAPEH"
BASE COUNT           76 a           50 c           62 g           82 t
ORIGIN      
        1 gtgttctgga gcggaaattg tggcagaatt tcccatagag aaaaatattt gaggaccaga
       61 ataatctggt ccggtggtta tttgttgtag tgtacgcatg gtatggccag acaatggatt
      121 acctgcaaca tttacgaagg cattctgagc taatctcagg acactttccg gaagtgttgt
      181 cagattatta ttcattagca cagccctgtt catactaaaa ggtaattccg gtagttgttc
      241 tataaaatta ttagccagag ccagcccttc
//
LOCUS       DADLTF010000468          269 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.474, whole genome shotgun sequence.
ACCESSION   DADLTF010000468 DADLTF010000000
VERSION     DADLTF010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.474"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..>269)
                     /locus_tag="JN749_004525"
     CDS             complement(<1..>269)
                     /locus_tag="JN749_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HAY7370640.1"
                     /translation="HDLTSLARDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPP
                     FSVFRDKFITMPGGMDYSRAIVVASVKEAPIGIEVIPAHVTEYLN"
BASE COUNT           65 a           64 c           75 g           65 t
ORIGIN      
        1 ttgttcagat attcagtgac gtgagcgggg atgacctcga taccaatcgg tgcttctttc
       61 actgacgcaa ccacgatggc gcgggaataa tccatcccgc caggcatggt gatgaatttg
      121 tcgcggaaaa cagaaaaggg cggtttattt tcagcgataa tttcctcgac acgtttagcg
      181 tgtgccggat gaaggttata aatgtccacg tccattgaac gagccagtac gccagtggct
      241 acgtcgcgcg ccagtgacgt cagatcgtg
//
LOCUS       DADLTF010000469          258 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.475, whole genome shotgun sequence.
ACCESSION   DADLTF010000469 DADLTF010000000
VERSION     DADLTF010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.475"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>258
                     /locus_tag="JN749_004526"
     CDS             <1..>258
                     /locus_tag="JN749_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAY7370641.1"
                     /translation="GNPEEVRELIDATPFAKKYTSGVLSFAEKELTPGGREKVMASFE
                     RVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKR"
BASE COUNT           70 a           58 c           81 g           49 t
ORIGIN      
        1 agggtaatcc ggaagaagtc cgggaactca tcgatgccac gccatttgcg aagaaataca
       61 cgtccggtgt tctgtcgttc gcggagaagg agctgacgcc gggaggacgt gaaaaagtga
      121 tggcgagctt tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt
      181 gggtggagca ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc
      241 tacagaccgg aaaacgcc
//
LOCUS       DADLTF010000470          257 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.476, whole genome shotgun sequence.
ACCESSION   DADLTF010000470 DADLTF010000000
VERSION     DADLTF010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.476"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>257
                     /locus_tag="JN749_004527"
     CDS             <1..>257
                     /locus_tag="JN749_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370642.1"
                     /translation="KVERMVQYTRNSFYIPLMTRLRPMGSTVDVETASRHGLRWLHDV
                     ANQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
BASE COUNT           66 a           71 c           71 g           49 t
ORIGIN      
        1 gtaaggtgga acggatggtg cagtacaccc gtaacagttt ttacatccca ctaatgactc
       61 gcctgcgccc gatggggagc actgtcgatg ttgaaacagc cagccgccac ggtctgcgct
      121 ggctgcacga tgtcgctaac caacgaaagc atgaaacaat ccaggcccgt ccctgtgatc
      181 gctggctcga agagcagcag tccatgctgg cactgcctcc ggagaaaaaa gagtatgacg
      241 tgcatcctgg tgaaaat
//
LOCUS       DADLTF010000471          255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.477, whole genome shotgun sequence.
ACCESSION   DADLTF010000471 DADLTF010000000
VERSION     DADLTF010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.477"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>255
                     /locus_tag="JN749_004528"
     CDS             <1..>255
                     /locus_tag="JN749_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HAY7370643.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRA"
BASE COUNT           70 a           62 c           74 g           49 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgc
//
LOCUS       DADLTF010000472          255 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.478, whole genome shotgun sequence.
ACCESSION   DADLTF010000472 DADLTF010000000
VERSION     DADLTF010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.478"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(<1..90)
                     /locus_tag="JN749_004529"
     CDS             complement(<1..90)
                     /locus_tag="JN749_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control protein D"
                     /protein_id="HAY7370644.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLA"
     gene            complement(87..>255)
                     /locus_tag="JN749_004530"
     CDS             complement(87..>255)
                     /locus_tag="JN749_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HAY7370645.1"
                     /translation="QSARIIQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGF
                     VPVIPLKVPVQ"
BASE COUNT           54 a           85 c           67 g           49 t
ORIGIN      
        1 cgccagccgt tcaataccgg caccgatgcg caccgatgtc cagccattcc actcccggcc
       61 atttacccgt agcgtgacgt tatcgttcat tgcactggca ccttcagagg gatcaccggc
      121 acaaagccgg gatgcgtaat ggcattacgc cggataatgt ccgcgtcacg cgccgcgtta
      181 tcaaaccagg tcgccgccag caccagcgcg ggtaaaacct catccggtgt gcgctgaatg
      241 atccgtgcag actgt
//
LOCUS       DADLTF010000473          253 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.479, whole genome shotgun sequence.
ACCESSION   DADLTF010000473 DADLTF010000000
VERSION     DADLTF010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.479"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            156..>253
                     /locus_tag="JN749_004531"
     CDS             156..>253
                     /locus_tag="JN749_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370646.1"
                     /translation="MLHEEKLARHQRKQAMYTRMVAFPAVKMFEEY"
BASE COUNT           75 a           54 c           67 g           57 t
ORIGIN      
        1 atacggtggt cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga
       61 ggcgactgga aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat
      121 cgataacttt gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac
      181 gtcatcaacg taaacaggcg atgtataccc gaatggtagc cttcccggcg gtgaaaatgt
      241 tcgaagagta tga
//
LOCUS       DADLTF010000474          248 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.480, whole genome shotgun sequence.
ACCESSION   DADLTF010000474 DADLTF010000000
VERSION     DADLTF010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.480"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..>248
                     /locus_tag="JN749_004532"
     CDS             <1..>248
                     /locus_tag="JN749_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAY7370647.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT           52 a           68 c           69 g           59 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgc
//
LOCUS       DADLTF010000475          244 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.denovo.481, whole genome shotgun sequence.
ACCESSION   DADLTF010000475 DADLTF010000000
VERSION     DADLTF010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.denovo.481"
                     /strain="EG0002"
                     /db_xref="taxon:623"
BASE COUNT           71 a           50 c           86 g           37 t
ORIGIN      
        1 ggcggccgga tggtataccg ccgggccatc gcgggactga atgtggaggg gctgaagaaa
       61 aacgcgcggg atatcagtct gctgaggtat atgccggcct gacacaggct aagtgtgccc
      121 gggaatcggt gcaagtataa aaaacagcct gttgcggtat aagaatcgac catgcagaga
      181 gagaaaacac cggagtggag ggaaaagcag aaaagctcac gtggcataag gcgtggacag
      241 cggt
//
LOCUS       DADLTF010000476        10431 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.guided.135, whole genome shotgun sequence.
ACCESSION   DADLTF010000476 DADLTF010000000
VERSION     DADLTF010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.guided.135"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            50..502
                     /locus_tag="JN749_004533"
     CDS             50..502
                     /locus_tag="JN749_004533"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7370648.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYVFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            748..1792
                     /locus_tag="JN749_004534"
                     /pseudo
     CDS             748..1792
                     /locus_tag="JN749_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1908..2468
                     /locus_tag="JN749_004535"
                     /pseudo
     CDS             1908..2468
                     /locus_tag="JN749_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570718.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2525..3982)
                     /gene="pepD"
                     /locus_tag="JN749_004536"
     CDS             complement(2525..3982)
                     /gene="pepD"
                     /locus_tag="JN749_004536"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HAY7370649.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTITEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4244..4702
                     /gene="gpt"
                     /locus_tag="JN749_004537"
     CDS             4244..4702
                     /gene="gpt"
                     /locus_tag="JN749_004537"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAY7370650.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4794..6038
                     /gene="frsA"
                     /locus_tag="JN749_004538"
     CDS             4794..6038
                     /gene="frsA"
                     /locus_tag="JN749_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HAY7370651.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPQGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6096..6497
                     /locus_tag="JN749_004539"
     CDS             6096..6497
                     /locus_tag="JN749_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HAY7370652.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6536..7591)
                     /gene="phoE"
                     /locus_tag="JN749_004540"
     CDS             complement(6536..7591)
                     /gene="phoE"
                     /locus_tag="JN749_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HAY7370653.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7879..8982
                     /gene="proB"
                     /locus_tag="JN749_004541"
     CDS             7879..8982
                     /gene="proB"
                     /locus_tag="JN749_004541"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAY7370654.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8994..10247
                     /gene="proA"
                     /locus_tag="JN749_004542"
     CDS             8994..10247
                     /gene="proA"
                     /locus_tag="JN749_004542"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAY7370655.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT         2543 a         2565 c         2720 g         2603 t
ORIGIN      
        1 aaaacactat cagtaagttg gagtcattac ccgtttttga atttatatta tgaataacat
       61 acaaataaga gattatcagc ctggcgattt tcagcaacta tgcgctattt tcattagagc
      121 ggttacgatg accgtcagtc agcattattc accacaacaa attgccgcct gggcgcagat
      181 tgacgaatct cgctggaagg agaaactcgc gaaatcacaa gtgcgggttg cagtcattaa
      241 tgcaaaactg gtcggcttta ttacctgcgt tgaacactat atcgatatgt tatttgttga
      301 ccctgaatac acccgccgtg gggttgccag cgctttgtta aaacctttta ttaagtctga
      361 atccgaactt acggtggacg caagcataac cgcaaaaccc ttttttgaac gttatgtttt
      421 tcagacagtt aagcagcagc gcgttgaatg ccggggagcg tggtttatta atttttatat
      481 gcgttataaa ccgcaacatt aaatccagct tgcaatgaaa ataacgcccg cctagtatgt
      541 gcgcgttttc ttatttcaca aactgcaagg aggtaaatca tgacaaatcc tttatcaatg
      601 actctttgca gacctttcca ggattaattc tttttttctt gccctggatt cgtctgccat
      661 ttcctgattt tttaattgtt actctcaata attcaagtcg cagcacatgc aacttgaagt
      721 atgacgagtc tatttacgga atggtatatg ggcaattata ttcgtccctt atccgatgcg
      781 gtatttacca tcgcatctga tgacctgtgg atcgagagtt tagcgatcca acaattacac
      841 accacggcaa atttacccaa catgcagcgc gtagtgggga tgccagattt acaccccgga
      901 cgcggctacc cgattggcgc agcgttcttc tccgcaggcc gtttttaccc agcactggtc
      961 ggcaatgata tcggctgtgg catggcgcta tggcaaacag atattctcgc tcgcaaatac
     1021 aacgccgata agtttgaaaa gcgattatca gctttggatg acgttgctga agaaagctgg
     1081 ctggaggaaa acctgccgtc agcatttgca cagcatccgt ggcgcagctc gcttggctct
     1141 atcggtggtg gcaatcattt cgctgaactg caacaagttg atcaaatcat caacgctgaa
     1201 ctgtttgcac tggcaggtct ggatgcgcag catctgcaac tgctggttca tagcggctcg
     1261 agggagctag ggcagtctat tttacagcgg catattgcct cgttttcgca tcatggtttg
     1321 cctgaaggca gtgacgacgc gctggcgttt gcgcgtttta atcgccacct gatcgctttg
     1381 cgcataatgc aacaggttaa ggccaccgga agtccggttc tggatgtggc gcataacttt
     1441 gttagcgcgt gtcgaatcgg tgatcaacaa ggggtcttac atcgtaaagg tgccacgcct
     1501 gatgactgtg gtctggtggt tattcccggt tcacgcggtg attactccag gctggttcag
     1561 gcccgtcagg agtgaggaaa cattgcattc gctggcgcat ggggctgggc gtaaatgggg
     1621 gcgcaccgag tgtaaagggc gtctggcagc gaaatacaca gcgacgcaac tctcgcgtac
     1681 tgaacttggc agtcgggtaa tttgtcgcga taaacaactc atcttcgaag aagcgccaca
     1741 agcctataaa tcggctgaaa gtgtggtgca atgtctgggc aggccggatt aattattcct
     1801 gtcgcgcggc tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa aggatgatct
     1861 tgctataact ctcctcagcg caggggccgg aagaatgttg tctcgcagtg aaaaaggcgc
     1921 tggacaggct aattaaagaa gctgcccgac aagacgtcgc ggtaacggtg ctggaaacag
     1981 aaacaggccg ctactctgac acgctgcgtt cggcgctgat ttctctggat ggcgacaacg
     2041 catgggcgtt aagcgaaagc tggtgtggca ctattcagtg gatttgcccg agcccgtatc
     2101 gcccacatca cgggcgtaaa aactggtttc tgggcattgg gcgttttacc gctgatgagc
     2161 aggagcaatc cgatgcgatc cggtatgaga cgctgcgttc gtcggggccg ggcggtcaac
     2221 atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt attagcgtga
     2281 aggttcagtc agagcgcagc cagcatgcta acaaacggct ggcgcgattg ctgattgcct
     2341 ggaagctgga tcagcagcaa taggaaaata gcgcggtgct gaaatcacag cggcgaatgt
     2401 tccatcacca gattgaacgt ggcaacccgc gacgaacgtt tacagggatg gcttttatcg
     2461 aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc agcaaatcaa
     2521 ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag taatgaccta
     2581 cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt ggcccgatag
     2641 aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga
     2701 taatctggat gttcggcgtc ttgttgaaca ggcgctgata ggtttcacgt accagatgca
     2761 tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct tcggttttcg
     2821 cgccagccag tttacccagc gaatccagca tgctcaccac gtagtcttta ccgctgtcga
     2881 tcagtgaacg gatcaggcag tgaatttcta cgttattgtc agtcatggtc accacaccga
     2941 cgttcaggga ggtttcgacc acaccttttg ccacgtcgga attgcggatc acaccgttcg
     3001 gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg gcagctttat
     3061 cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc agctcgtttt
     3121 tcaggatctc ctgataggta ttcaccagag atttcagtgc gtcgacttta tcagctgtga
     3181 cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat
     3241 cgataaggcg caggtccagt tcttctgcat gacccgccag gaagcgcacc agcagtttgt
     3301 tggcattacc caggccaacg tggatttcac cgccggagtg accgcctttc agacctttta
     3361 aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg
     3421 aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct tcggagtcgg
     3481 tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac
     3541 cggcttcttc ggttatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag
     3601 ccagaaccgc cagtgcagag gccataccaa tgccgttatc cgcacccagc gtggtgccgc
     3661 gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg aagtcatgca
     3721 cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg accggtttac
     3781 gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc tgatcgcgtt
     3841 cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat
     3901 gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag
     3961 ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg
     4021 caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtatt
     4081 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     4141 tgaaatactg gtttttagtg cgccagatat ctataatctc gcgcaaccta ttttcccctc
     4201 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     4261 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     4321 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gggcgttact
     4381 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     4441 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     4501 tattgatgac ctggtggata ccggtggtac tgctgttgcg attcgtgaaa tgtatccaaa
     4561 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     4621 tgttgatatc ccgcaagata cctggatcga acagccgtgg gatatgggcg tcgtattcgt
     4681 cccgccaatc tccggtcgct aatcttttca acgcctggca ttgccgggcg ttgttctttt
     4741 taacttcagg caggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     4801 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     4861 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggctctt gatggtaaaa
     4921 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggtattgacc
     4981 cacgtgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgctgaa cgtaccgacg
     5041 atgatttata cgataccgtg attggctacc gtggtggcaa ctggatttat gagtgggcca
     5101 cccaggcgat ggtgtggcaa caaaaagcct gtacggaaga agatccgcaa ctcagtggtc
     5161 gacactggct gcatgcggct acgttgtaca acattgccgc ctatcctcat ctgaaaggag
     5221 atgacctggc cgagcaagcg caggctttat caaaccgcgc ctatgaagag gccgcccagc
     5281 gtctgccggg cacgatgcgg cagatggagt ttaccgtacc cggcggtgcg cccatcaccg
     5341 gctttttgca tatgccgcaa ggcgatggcc cgttcccgac agtattaatg tgtggtggtc
     5401 tggatgcgat gcagacggac tattacagcc tgtatgaacg ttattttgcg ccgcgcggca
     5461 ttgcgatgct gactattgat atgccgtcgg tgggcttttc ttcaaaatgg aagctcaccc
     5521 aggactccag cctgttgcat cagcacgtct taaaggcgct gcctaacgta ccgtgggtgg
     5581 atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc taacgttgcc gtgcgtctgg
     5641 cataccttga atcgccgcgt ctgaaagcgg ttgcctgtct tggtccggta gttcataccc
     5701 tgttgagtga ttttaagtgc cagcaacagg tgccggaaat gtatcttgac gttctggcga
     5761 gtcgtttggg gatgcatgat gcttccgacg aagcgttacg cgtggagctg aatcgctatt
     5821 cattaaaagt gcaaggattg ctggggcgtc gctgcccaac gccaatgtta tcaggctact
     5881 ggaagaacga tccgttcagc ccggaagagg actcacgctt aatcacctca tcatctgctg
     5941 acggtaaatt attagagatc ccatttaacc cggtgtatcg gaattttgac aaaggtcttc
     6001 aggaaatcac cggctggatc gaaaaacgct tgtgttaaaa atttgctaaa ttttgccaat
     6061 ttggtaaaac agttgcatca caacaggaga tagcaatgac gttaccgagt ggacacccga
     6121 agagcagatt gatcaaaaaa tttaccgcac taggcccgta tattcgtgaa ggtaagtgcg
     6181 aagataatcg attctttttc gattgtctgg ctgtatgcgt caacgtgaaa ccggcaccgg
     6241 aagtgcgtga attctggggc tggtggatgg agctggaagc gcaggaatcc cgttttacct
     6301 acagctacca gtttggtctg ttcgataaag caggcgactg gaagagtgtt ccggtaaaag
     6361 atactgaagt ggttgaacga ctggagcaca ccctgcgtga gtttcacgag aagctgcgtg
     6421 aactgctgac gacgctgaat ctgaagctgg aaccggcgga tgattttcgt gatgaaccgg
     6481 tgaagttaac ggcgtgagtg aaatgtgccg gatgcatcac atccggcaat attcattaaa
     6541 actgatacgt catcccaacc gcgacagtat catcattatt aatattcagc ttattatcgc
     6601 tatccagttt gttgatttta taatcaacaa acgctgacat atttttgttg aaataatacg
     6661 tagcaccgac gtcgatataa ttgaccagat cttcatcacc gatgccctca atatctttcc
     6721 ctttcgataa gacataaccc agcgatggac gcagaccaaa gtcaaactgg tattgagcga
     6781 ccgcttcaaa gttctgtgtc ttattggcaa agccgccagt tattggcgtc attttgcgtg
     6841 tttcagaata gaaagttgcc agataaatat tattggcatc gtatttcaga cctgttgccc
     6901 atgcttctgc acgcttgcct gtgccacggc tttgcaggtt ctgctcgttg gtgcgatctg
     6961 agttggtata ggccccacta atggcgaaat cgctgccgcc aaagtcatat gtcaatgacg
     7021 tgccgaagcc atcgccgttt tgctttttaa cgtcgcggtt ttcgtttttc ccttgatatt
     7081 gcagggttaa gttcaggcca tcgataacgc cgaagaagtc ggtgttccga tacgtcgcca
     7141 gaccgctggc gcgtttggtc ataaagttgt cggtctgcgc cgaggaatcg ccaccaaatt
     7201 ccgggaacat atcggtccag gcttccacgt catacaacgc gcccaggtta cgaccataat
     7261 cgaaagaacc caaatcttta tatttcaacc cggcaaaagc gagacgcgtt ttttgctgtg
     7321 cagtatcact ctccgcttta tttccggcaa actccgcttc ccaccgacca taaccagtca
     7381 gttgatcgtt aatttgagtt tcacctttaa aaccaaaacg gatataactc tggtcgccat
     7441 ctttactggc gttatcactc atataatgca tggctttaac tttgccatag acatccagtt
     7501 tattaccgtc tttattatat atttctgcag cctgtacaga tgcagatgcc acaatgccca
     7561 tcaccactaa tgccagagtg ctctttttca ttttcattcc tgattttaat taacgcgcga
     7621 atattcagcg ggagagcccc gttgaaaaca ggaaagtttt taacctgaga ttgttaaaga
     7681 tatattacag attaatgata ttctaaaaat gtggtaattt attaaatctg taataaaagc
     7741 gtaaacaact gccgctacgc ttgctgatcc cgcgcaacaa aacgccatgc tttgctcgca
     7801 gatggttggc aaccgacgac agtcctgcta aaacgttcgt ttgatatcat ttttcctaaa
     7861 attgaatggc agagaatcat gagtgacagc cagacgctgg tggtaaaact cggcaccagt
     7921 gtgctaacag gcggatcgcg ccgtctgaac cgtgcccata tcgttgaact tgttcgccag
     7981 tgcgcgcagt tacatgccgc cgggcatcgg attgttattg tgacgtcggg cgcgatcgcc
     8041 gccggacgtg agcacctggg ttacccggaa ctgccagcga ctatcgcctc gaaacaactg
     8101 ctggcggcgg tagggcagag tcgactgatt caactgtggg aacagctgtt ttcgatttat
     8161 ggcattcacg tcgggcaaat gctgctgacc cgtgctgata tggaagaccg tgaacgcttc
     8221 ctgaacgccc gcgacaccct gcgtgcgttg ctcgatcaca atatcgttcc ggtaatcaat
     8281 gagaacgatg ctgtcgctac ggcagagatt aaggtcggcg ataacgataa cctttctgcg
     8341 ctggcggcga ttctggcggg tgccgataaa ctgttgctgc tgaccgatca aaaaggtttg
     8401 tataccgctg atccgcgcag caatccgcag gcagaactga ttaaagatgt ttacggcatt
     8461 gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag gcctcggaac tggcggcatg
     8521 agtactaaat tgcaggccgc tgacgtggct tgccgtgcgg gtatcgacac cattattgcc
     8581 gcgggcagca agccgggcgt tattggtgat gtgatggaag gcatttccgt cggtacgctg
     8641 ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct ggattttcgg tgcgccgccg
     8701 gcgggtgaaa tcacggtaga tgaaggggca actgccgcca ttctggaacg cggcagctcc
     8761 ctgttgccga aaggcattaa aagcgtgact ggcaatttct cgcgtggtga agtcatccgc
     8821 atttgcaacc tcgaaggtcg cgatatcgcc cacggtgtca gtcgttacaa cagcgatgca
     8881 ttacgccgta ttgccggaca ccactcgcaa gaaattgatg caatactggg atatgaatac
     8941 ggcccggttg ccgttcaccg tgatgacatg attacccgtt aaggagctgg ccgatgctgg
     9001 aacaaatggg cattgccgcg aagcaagcct cgtataaatt agcgcaactc tccagccgcg
     9061 aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga agcacaaagc gaaatcatcc
     9121 tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa tggccttagc gaagcgatgc
     9181 ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat tgccgacgat gtacgtcagg
     9241 tgtgcaacct cgccgatccg gtggggcagg taatcgatgg cggcgtactg gacagcggcc
     9301 tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg cgtgatttat gaagcgcgcc
     9361 cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac cggtaatgcg gtgatcctgc
     9421 gtggtggtaa agaaacctgt cgcactaacg cggcaacggt ggcggtgatt caggacgccc
     9481 tgaaatcctg tggcttaccg gcgggtgccg tgcaggcgat tgataatcct gaccgtgcgc
     9541 tggtcagtga aatgctgcgt atggataaat acatcgacat gctgatcccg cgtggtggcg
     9601 ctggtttgca taaactgtgc cgtgaacagt cgacaatccc ggtgatcaca ggtggtatag
     9661 gcgtatgcca tatttacgtt gatgaaagcg cagagatcgc tgaagcatta aaagtgatcg
     9721 tcaacgcgaa aactcagcgt ccgagcacat gtaatacggt tgaaacgttg ctggtgaata
     9781 aaaacatcgc cgatagcttc ctgcccgcat taagcaaaca aatggcggaa agcggcgtga
     9841 cattacacgc agatgcagct gcgctgacac agttgcaggc aggccctgcg aaggtggtgg
     9901 cggttaaagc cgaagagtat gacgatgagt ttctgtcatt agatttgaac gtcaaaatcg
     9961 tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca cggcacacaa cactccgatg
    10021 cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt taacgaagtg gattcgtccg
    10081 ctgtttacgt taacgcctct acgcgtttta ccgacggcgg ccagtttggt ctgggtgcgg
    10141 aagtggcggt aagcacacaa aaactccacg cgcgtggccc aatggggctg gaagcactga
    10201 ccacttacaa gtggatcggc attggtgatt acaccattcg tgcgtaaata aaaccgggtg
    10261 atgcaaaagt agccatttga ttcacaaggc cattgacgca tcgcccggtt agttttaacc
    10321 ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag ggccgatata gctcagttgg
    10381 tagagcagcg cattcgtaat gcgaaggtcg taggttcgac tcctattatc g
//
LOCUS       DADLTF010000477         9059 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.guided.149, whole genome shotgun sequence.
ACCESSION   DADLTF010000477 DADLTF010000000
VERSION     DADLTF010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.guided.149"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            <1..290
                     /locus_tag="JN749_004543"
     CDS             <1..290
                     /locus_tag="JN749_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAY7370656.1"
                     /translation="RDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCARVF
                     IQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA"
     gene            complement(472..648)
                     /locus_tag="JN749_004544"
     CDS             complement(472..648)
                     /locus_tag="JN749_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000954592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HAY7370657.1"
                     /translation="MLTDTKLRNLKPRDKLYKVNDREGLYVGVASENGIYGHSRFCNT
                     DFDDKLACLNLSGV"
     gene            complement(877..954)
                     /locus_tag="JN749_004545"
                     /pseudo
     CDS             complement(877..954)
                     /locus_tag="JN749_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            978..1793
                     /gene="sul2"
                     /locus_tag="JN749_004546"
     CDS             978..1793
                     /gene="sul2"
                     /locus_tag="JN749_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HAY7370658.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1854..2657
                     /locus_tag="JN749_004547"
                     /note="aph(3'')-Ib"
     CDS             1854..2657
                     /locus_tag="JN749_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HAY7370659.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2657..3493
                     /locus_tag="JN749_004548"
     CDS             2657..3493
                     /locus_tag="JN749_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HAY7370660.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3465..4004)
                     /locus_tag="JN749_004549"
     CDS             complement(3465..4004)
                     /locus_tag="JN749_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAY7370661.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMA
                     EQGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARR
                     MAESAALLVDEVLPEQPMRQWVLSFPFQLRFLFGVVCGKGRNPTLRLWPAIFSGEIDV
                     FPGDRRSRLISSMTSVAPA"
     gene            complement(4214..5074)
                     /locus_tag="JN749_004550"
     CDS             complement(4214..5074)
                     /locus_tag="JN749_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A broad-spectrum beta-lactamase TEM-1"
                     /protein_id="HAY7370662.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(5257..5814)
                     /locus_tag="JN749_004551"
     CDS             complement(5257..5814)
                     /locus_tag="JN749_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7370663.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            5978..8986
                     /locus_tag="JN749_004552"
     CDS             5978..8986
                     /locus_tag="JN749_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAY7370664.1"
                     /translation="MPVDFLTTEQELNYGRYVAEPNDVQLARYFHLDERDLAFINQRR
                     GRHNRLGIALQLTTARFLGTFLTDLTRVLPGVQQFVAVQLNIRRPEVLTRYAERVTTL
                     REHTALIKEYYGYHEFGDFPWSFRLKRLLYTRAWLSNERPGLMFDFATAWLLQNKVLL
                     PGATTLVRLVSEIRERANQRLWKKLAALPDSWQTARVTELLDIPEGQRTSPLEQLKKG
                     PVTVSGPAFTEALERYIRLRNLEFSRLNFTGLPAIQLRNLARYAGMASVKYIARMPQQ
                     RKLAVLTAFVKAQETAALDEAVDVLDMLILDITRAAKKTGQKKRLRTLKDLDRAALIL
                     ARACSLLLDEQADDAELRETIFNSIPKSRLAESVSKVNELARPQNNNFHDEMVEQYGR
                     VKRFLPAVLRDLHFQAAPAGEHTLSAIHYLTELNGSKKRILDNAPEHIITGPWKRLVY
                     DAEGRIQRAGYSLCLLERLQDALRRRDIWLENSDRWGNPREKLLQGEEWQAQRVPVCR
                     ALGHPTDGHKGVQQLAVQLDETWKTVASRFEGNAEVHICHDGKHPSLTISSLEKLEEP
                     TSLHRLNSRVRLLLPPVDLTELLLEIDARTGFTREFTHVSESGARAQDLHISLCAVLM
                     AEACNIGLEPLIKHNIPALTRHRLSWVKQNYLRAETLVSANARLVDFQSTLELAGRWG
                     GGEVASADGMRFVTPVKTVNSGPNRKYFGSGRGITWYNFVSDQYSGFHGIVVPGTLRD
                     SIFVLEGLLEQQTGLNPVEIMTDTAGTSDIIFGLFWLLGYQFSPRLADAGEAVFWRVD
                     KSANYGALDELARGCADLSKAEDQWDEMMRTAGSLKLGTIHASELIRSLLKSSRPSGL
                     AQAIMEVGRVNKTLYLLNYIDDEDYRRRILTQLNRGEGRHAVARAICYGQRGEIRKRY
                     REGQEDQLGALGLVTNAVVLWNTLYMQEALSWIRSNGEEIGVEDIARLSPLMHGHINM
                     LGHYTFTLPEDILKGELRELNFNLNNELTS"
BASE COUNT         2108 a         2373 c         2415 g         2163 t
ORIGIN      
        1 gccgtgactt tgcggcggca acagggctgg taccccgaca gtacagcacg ggaggtcgga
       61 cgacattgtt agggattagc aagcggggca acaaaaagat ccgaactttg ttggttcagt
      121 gtgccagggt attcatacaa aaactggaac accagtctgg caagttggcc gactgggtca
      181 gggagttgtt gtgtcggaaa agcaactttg tcgtcacctg tgctctggca aacaagctgg
      241 ccagaatagc ctgggcactg acggcgcgac agcaaactta cgaagcataa aggcagaaat
      301 acaccagttt aaacaatcat tcatctggtt ttgcgaatac tgatattgat gatactaacg
      361 gcccaccggc ctgttgagga acctgtaaaa cggaaaggct cattgaagcc gtatattttc
      421 tggaggttca tcaggcgcgg aactcatcga ggcgcgggaa taaaatccca ttcagacgcc
      481 ggatagattc aagcaagcca acttgtcgtc aaaatcggtg ttgcaaaaac gggagtgacc
      541 atagattccg ttttctgagg cgacccccac atagagacct tcccggtcat tcactttgta
      601 gagtttgtcc ctgggcttga gattgcgcag cttggtatcg gtcagcatgt accttgtcct
      661 tcttcgtctc cgatacccac tctattgtaa acaagacatt tttatctttt atattcaatg
      721 acttattttc ctgctaattg gtaataccat gaaaaatacc atgctcagaa aaggcttaac
      781 aatattttga aaaattgcct actgagcgct gccgcacagc tccataggcc gctttcctgg
      841 ctttgcttcc agatgtatgc tattctgctc ctgcagctaa tggagcaccg caaacaggtt
      901 actcgcctgg ggattccctt tcgacccgag catccgtatg agactcatgc tcgattatta
      961 ttattataga agcccccatg aataaatcgc tcatcatttt cggcatcgtc aacataacct
     1021 cggacagttt ctccgatgga ggccggtatc tggcgccaga cgcagccatt gcgcaggcgc
     1081 gtaagctgat ggccgagggg gcagatgtga tcgacctcgg tccggcatcc agcaatcccg
     1141 acgccgcgcc tgtttcgtcc gacacagaaa tcgcgcgtat cgcgccggtg ctggacgcgc
     1201 tcaaggcaga tggcattccc gtctcgctcg acagttatca acccgcgacg caagcctatg
     1261 ccttgtcgcg tggtgtggcc tatctcaatg atattcgcgg ttttccagac gctgcgttct
     1321 atccgcaatt ggcgaaatca tctgccaaac tcgtcgttat gcattcggtg caagacgggc
     1381 aggcagatcg gcgcgaggca cccgctggcg acatcatgga tcacattgcg gcgttctttg
     1441 acgcgcgcat cgcggcgctg acgggtgccg gtatcaaacg caaccgcctt gtccttgatc
     1501 ccggcatggg gttttttctg ggggctgctc ccgaaacctc gctctcggtg ctggcgcggt
     1561 tcgatgaatt gcggctgcgc ttcgatttgc cggtgcttct gtctgtttcg cgcaaatcct
     1621 ttctgcgcgc gctcacaggc cgtggtccgg gggatgtcgg ggccgcgaca ctcgctgcag
     1681 agcttgccgc cgccgcaggt ggagctgact tcatccgcac acacgagccg cgccccttgc
     1741 gcgacgggct ggcggtattg gcggcgctga aagaaaccgc aagaattcgt taactgcaca
     1801 ttcgggatat ttctctatat tcgcgcttca tcagaaaact gaaggaacct ccattgaatc
     1861 gaactaatat tttttttggt gaatcgcatt ctgactggtt gcctgtcaga ggcggagaat
     1921 ctggtgattt tgtttttcga cgtggtgacg ggcatgcctt cgcgaaaatc gcacctgctt
     1981 cccgccgcgg tgagctcgct ggagagcgtg accgcctcat ttggctcaaa ggtcgaggtg
     2041 tggcttgccc cgaggtcatc aactggcagg aggaacagga gggtgcatgc ttggtgataa
     2101 cggcaattcc gggagtaccg gcggctgatc tgtctggagc ggatttgctc aaagcgtggc
     2161 cgtcaatggg gcagcaactt ggcgctgttc acagcctatc ggttgatcaa tgtccgtttg
     2221 agcgcaggct gtcgcgaatg ttcggacgcg ccgttgatgt ggtgtcccgc aatgccgtca
     2281 atcccgactt cttaccggac gaggacaaga gtacgccgca gctcgatctt ttggctcgtg
     2341 tcgaacgaga gctaccggtg cggctcgacc aagagcgcac cgatatggtt gtttgccatg
     2401 gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc acgggtctga
     2461 tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc atgattgcta
     2521 acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct gtcctattca
     2581 atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg cgattggacc
     2641 ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc acgtttcgca
     2701 acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat tgccagacgg
     2761 gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg aactgcgcgg
     2821 ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg gtcgtgagaa
     2881 caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg ttgccgagca
     2941 cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt atgccgcatc
     3001 tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag ctttgtttca
     3061 gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg cggcgattat
     3121 agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg atctgcatca
     3181 tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg gtctggtcgg
     3241 tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag acgacctttg
     3301 tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc tggacgtcga
     3361 tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag cttggaacgc
     3421 ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc aggtgcgaca
     3481 gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc aatctcaccg
     3541 gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca aacgacccca
     3601 aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg ttgttcaggc
     3661 agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc acagctcggg
     3721 cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca agactcgcag
     3781 cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc aaattcccgt
     3841 tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa tgccgggtaa
     3901 tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg gtaacgacca
     3961 gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg cgtccttgca
     4021 atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttagggg tctgacgctc
     4081 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4141 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4201 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     4261 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     4321 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     4381 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4441 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4501 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     4561 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4621 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggcag
     4681 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4741 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4801 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcacca catagcagaa
     4861 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4921 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4981 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5041 gaataagggc gacacgaaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5101 gcatttacca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5161 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     5221 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     5281 aattttataa accgtggagc gggcaatact gagctgatga gcaatttccg ttgcaccagt
     5341 gcccttctga tgaagcgtca gcacgacgtt cctgtccacg gtacgcctgc ggccaaattt
     5401 gattcctttc agctttgctt cctgtcggcc ctcattcgtg cgttctagga tcctccggcg
     5461 ttcagcctgt gccacagccg acaggatggt gaccaccatt tgccccatat caccgtcggt
     5521 actgatcccg tcatcaatga accggactgc cacgccctga gcgtcaaatt cctttatcag
     5581 ttggatcata tcggcagtgt cgcggccaag acggtcgagc ttcttaacca gaatgacatc
     5641 accttcctcc accttcatcc tcagcaaatc cagcccttcc cggtctgttg aactgccgga
     5701 tgccttatcg gtaaatatac ggtttgcttt cacacctgcg tctttgagtg ctctgacctg
     5761 aagatcaaga gactgctgac tggttgagac ccgagcgtaa ccaaaaagtc gcataaaaat
     5821 gtaccttaaa tcgaatatcg gacaactcat gtctattatt acaaatttac gatttaatag
     5881 acatattaat gtaacagttt tacgatgtcc gataatttat aatatttcgg acggttgcga
     5941 aattgttaat tcatgaccat caggcaggga ggccggtatg cccgtcgatt ttctgaccac
     6001 tgagcaggaa ctgaattatg gtcgctatgt tgcagaaccc aatgacgtgc agctggcgcg
     6061 ctattttcat cttgatgagc gggatcttgc cttcattaac cagcggcggg gaaggcataa
     6121 ccggctggga attgcgcttc agctcaccac cgcccgtttt ctgggcacct ttctgacaga
     6181 cttaacccgg gttctgccag gtgttcagca atttgtcgcg gtgcagctta atattcgccg
     6241 tccggaagtc cttacccgct acgcagaacg ggttaccacc ctcagagagc atactgcgct
     6301 gattaaggaa tattacggtt accatgaatt tggtgatttc ccctggtctt tccgcctgaa
     6361 gcgcctgctg tacacccgcg catggctcag taatgagcga cccggcctta tgtttgattt
     6421 tgccaccgcg tggctgcttc agaataaggt cctgctgccc ggagcaacca cacttgtacg
     6481 tctcgtcagt gaaatacgcg aacgggcaaa tcagcggctg tggaagaaac tggccgcgtt
     6541 gccggacagc tggcagaccg cccgcgtgac ggagcttctg gacattcctg aggggcagcg
     6601 tacttcaccg ctggagcaac tgaaaaaggg accggtcacc gtcagcggcc cggcatttac
     6661 cgaagcgctg gagcggtata tccggctgcg aaacctggaa ttttcccgac tgaattttac
     6721 cggtctgcct gctatacaac tgcgtaattt ggcccgttac gcaggcatgg cgtcggtaaa
     6781 atatatcgcg cgaatgccac agcagagaaa actggccgta ctcacagcat tcgttaaagc
     6841 gcaggagact gcggcgctgg atgaggccgt tgatgtgctc gatatgctga tactggatat
     6901 cactcgtgcg gcgaagaaaa cggggcagaa aaaacggctc aggacgctta aagatcttga
     6961 tcgtgctgcg ctaatattgg cgcgggcatg ttcattgttg ctggatgaac aggctgacga
     7021 tgctgaactg agggagacca tattcaacag cataccgaaa agcaggctgg cagaatccgt
     7081 cagcaaggta aatgagctgg cccggcctca gaacaacaat ttccatgacg aaatggtgga
     7141 gcagtacggg cgggtaaagc gctttcttcc ggcggtattg cgcgacctgc atttccaggc
     7201 agcgccagcc ggagaacata cgttgtccgc cattcattat ctgaccgaac tgaacggctc
     7261 gaaaaagcgc atcctggaca atgcgcctga acatattatt accggcccct ggaaacgcct
     7321 ggtatacgat gcggagggcc ggatacagcg tgccggttac tcgctttgtc tgctggagcg
     7381 ccttcaggat gcactacgtc gaagggacat ctggctcgaa aacagcgatc gctggggaaa
     7441 tccccgcgag aagctgttgc agggggaaga atggcaggct cagcgggtcc ccgtttgccg
     7501 ggcgctggga catcccactg atggacataa aggcgtgcaa cagttggcgg tccagctaga
     7561 tgaaacctgg aaaaccgtcg catcccgctt tgaaggaaat gcggaggttc atatttgcca
     7621 tgacggtaaa catccttccc tgactatcag cagcctggag aaactggagg agccaacatc
     7681 gttgcatcgt ctcaacagtc gggtaaggct gctactcccg ccagtagatt tgacggaact
     7741 gttgcttgaa atagatgcca gaacgggatt tacacgtgag tttacgcatg tcagtgaatc
     7801 cggggcccgg gcacaggatc tgcacatcag cctgtgcgcg gtcctgatgg cagaggcctg
     7861 caatatcggg ctggaaccgc tgataaagca caatataccg gcgctgacgc gccaccggct
     7921 tagctgggta aaacagaatt acctccgggc agaaacgctg gtcagcgcca atgcccgcct
     7981 ggttgatttt cagtccacac tggagcttgc tggccgctgg ggtggtggcg aagtggcttc
     8041 agctgatggc atgcgctttg tcacgccggt gaaaaccgtc aattcaggac ctaacagaaa
     8101 atattttggt tccggacgtg gcatcacctg gtacaacttc gtctctgatc agtactctgg
     8161 attccacggc atcgttgtcc ccggcacatt acgagattcc atttttgtgc tggaaggcct
     8221 tctggagcag cagacagggc tgaatccggt tgagatcatg acagacacag ccggtaccag
     8281 cgacattatt tttggcctct tctggctgct gggataccag ttttcccccc ggcttgccga
     8341 tgccggtgaa gcggtattct ggcgagtgga taaatcggca aattacggtg cactggacga
     8401 actggcacgt ggttgtgccg atctgtcgaa agccgaggat cagtgggatg agatgatgcg
     8461 aaccgccggt tcgctgaaac tgggcaccat tcatgcttca gaactcattc gctctttgct
     8521 gaaaagctcg cgcccatcag ggctggcaca ggcgatcatg gaagtggggc gcgtcaacaa
     8581 gacgctgtac cttcttaatt atattgatga tgaggattat cgtcggcgga tcctgacgca
     8641 gctaaaccgg ggggaaggcc gccatgctgt ggcgagggcg atctgctacg ggcagcgcgg
     8701 tgagatcaga aagcgctatc gtgaaggtca ggaagatcag ctgggggcac tgggcctggt
     8761 cactaacgca gtggtactgt ggaacacact ttatatgcag gaagccctga gctggatacg
     8821 cagtaatgga gaagaaatcg gggttgaaga tatcgcccgg ttgtccccac tgatgcacgg
     8881 gcatatcaat atgctggggc attatacgtt tacgctaccg gaggatattt tgaaggggga
     8941 actgagagaa ctaaatttca atttaaacaa tgaattaact tcttagcgta cgttttcgtt
     9001 ccattggccc tcaaacccca tcttgtaaca atcaggcata ccacggagct tgtttgctt
//
LOCUS       DADLTF010000478         4560 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.guided.224, whole genome shotgun sequence.
ACCESSION   DADLTF010000478 DADLTF010000000
VERSION     DADLTF010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.guided.224"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            26..1159
                     /gene="blaEC"
                     /locus_tag="JN749_004553"
     CDS             26..1159
                     /gene="blaEC"
                     /locus_tag="JN749_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HAY7370665.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            1248..1781
                     /locus_tag="JN749_004554"
     CDS             1248..1781
                     /locus_tag="JN749_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HAY7370666.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2007)
                     /locus_tag="JN749_004555"
                     /pseudo
     CDS             complement(1778..2007)
                     /locus_tag="JN749_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="member of the SMR family of proton-dependent drug
                     efflux transporters; quaternary ammonium compound efflux
                     pump; confers resistance to cetylpyridinium,
                     cetyldimethylethyl ammonium and cetrimide cations;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
     gene            complement(2183..2329)
                     /gene="ecnB"
                     /locus_tag="JN749_004556"
     CDS             complement(2183..2329)
                     /gene="ecnB"
                     /locus_tag="JN749_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HAY7370667.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2440..2565)
                     /gene="ecnA"
                     /locus_tag="JN749_004557"
     CDS             complement(2440..2565)
                     /gene="ecnA"
                     /locus_tag="JN749_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HAY7370668.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(2617..3183)
                     /gene="efp"
                     /locus_tag="JN749_004558"
     CDS             complement(2617..3183)
                     /gene="efp"
                     /locus_tag="JN749_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAY7370669.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3225..4253
                     /gene="epmB"
                     /locus_tag="JN749_004559"
     CDS             3225..4253
                     /gene="epmB"
                     /locus_tag="JN749_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HAY7370670.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1172 a         1147 c         1131 g         1110 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttactt ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca ccgctggcgg
      421 cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg ccccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc ccccagaaga
      661 aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc caggggcgtt
      721 agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct gggtgcaaag
      781 caatttaccc ccccttgata tcaatgagaa aacgcttcaa caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtcaat cctgacagca tcattaacgg cagtgacaat aaaattgcac tggcagcacg
      961 cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 gggggcgacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 cgtgatgctg gctaacaaaa actatcccaa tccagagaga gtcgccgccg cctgtcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgtagttct cctacgccgc
     1321 cgcgtggcgt gaccgtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccagacg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 caaacggcta aagccgtggg tatatttcag gccaacggcc cataccactt ccagcagacc
     1981 agcaataact aagataatcc aggacatatc aggctccgga aatttggggc cgtccccggt
     2041 gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt tgaacggata ttttgccagg
     2101 tggcagggga atttcaacta ttacctttat gacgatcgca gaaaaaacaa acggcacgac
     2161 actgctgtcg taccgttatt gcttattgct gcgctttcgt tgctgcgcca gaaatcgcgt
     2221 taccgccatc agaaatgtct tcaccaacgc cacgcgtggt gttgcaggca gttaatactg
     2281 ttgaaagcac cagaacagaa aagatcgctg caattgtctt cttcaccata acgtcttcct
     2341 tttaggtaat gttatttatg tttgcctata gcaaattaat aatagacaac atcgccaaaa
     2401 atgatggaaa tctgatgatt tttcggaaga gaagaaaaat tagctggcag cgcgagagat
     2461 ggagttgccg agatgtttga tgtcttcgcc gaaaccgcaa gcggtgttac agcccgtgag
     2521 cagcgtgctg gcaagcaaaa caagaacgat aaggcgtttc atcatctctg acctgaataa
     2581 gtgatggtgc agcctgcagg ccgcaccaca accgcattac ttcacgcgag agacgtattc
     2641 accagagcgg gtatccactt tgatgacttc gccgatttgt acgaacagcg gaactttaac
     2701 cacagcgcca gtagacaggg tagccggttt gccgccagta cctgcggtat cacctttcag
     2761 gcccggatcg gtatcaacga tttccagttc aacgaagttc ggcggagtaa cggagatcgg
     2821 ctgaccattc cacagagtta cgatacactc tgcctgatcc agcagccatt tagcgttgtc
     2881 gccaattgct tttgcatcag cagacagctg ctcgaaagtt tcgttgttca tgaagtgcca
     2941 gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg acaacatcag cgccttcagc
     3001 ggaatcagta gatttgaagg ttttttctac gcgagtaccg gtcagcagac gacgcagttt
     3061 aacgcgagca aatgcctggc ctttacccgg ttttacgaat tcactcgctt caaccgcgta
     3121 aggttcgccg tctaacatga ttttaagacc agcacgaaaa tcgttgctat agtacgttgc
     3181 cataaggccc tctgaaattt gttaattggt agctaagcca caaaatggcg catattgtaa
     3241 ccctaaatac cccatccaga gaagattggt taacgcaact tgccgatgtt gtgaccgatc
     3301 ctgatgaact tctgcgtctt ttgaatatag acgcggacga aaaactgttg gccggacgca
     3361 gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt tatcgatcgc atggagaaag
     3421 gcaatccgga cgatcctctt ttgcgtcagg tacttacctc gcaagatgag tttgtcgtcg
     3481 cgtccggatt ctccaccgac ccactggaag aacagcacag cgtagtgcct ggtttgttgc
     3541 ataaatacca caaccgggcg cttttgctgg tcaaaggcgg ctgcgcggta aattgccgct
     3601 attgcttccg tcgccacttc ccctatgccg aaaatcaggg caacaagcgt aactggcaga
     3661 ctgcacttga gtatgttgct gcgcatccgg aactggacga gatgattttc tccggcggcg
     3721 atccgctgat ggcgaaagat cacgagctgg actggttgct cacacaactg gaagccatcc
     3781 cgcatatcaa acgtctgcgg attcacagcc gtctgccgat tgtgatcccg gcacgtatca
     3841 ccgaggcgct ggttgaacgc tttgcccgtt ctacgctgca aatcttgctg gtgaatcaca
     3901 tcaatcatgc caatgaggta gatgaaacgt tccgtcaggc gatggctaaa ttgcgccgtg
     3961 tcggtgtcac cctgcttaac cagagcgttc tgttacgtgg tgtgaacgat aacgcacaaa
     4021 cgttggcaaa cctgagtaat gcgttgttcg atgccggcgt gatgccgtat tacctgcatg
     4081 tgctagataa agtacagggc gcggcgcatt ttatggtgag tgatgacgaa gcacggcaga
     4141 ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt gccgaaactg gcgcgagaaa
     4201 ttggcggcga acccagcaaa acgccgctgg atctccagct acgccagcag taactgatta
     4261 aacagggaag ttaaacatta aaattgcctg atgcgctacg cttatcaggc ctacatcgtg
     4321 cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt acgccgcatc
     4381 cggcaagagc aacaaacgca agtacagcaa cctgtcatta aactgagaaa tttaatcttt
     4441 gcacgttgca taacctttaa ttattcatat gcattgctgt tgttactttt tatatagcta
     4501 acaccaataa ttaacctttc ttttcggtaa tgactccaac ttactgatag tgttttatgt
//
LOCUS       DADLTF010000479         4251 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.guided.239, whole genome shotgun sequence.
ACCESSION   DADLTF010000479 DADLTF010000000
VERSION     DADLTF010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.guided.239"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            70..285
                     /locus_tag="JN749_004560"
     CDS             70..285
                     /locus_tag="JN749_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAY7370671.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="JN749_004561"
     CDS             278..664
                     /locus_tag="JN749_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HAY7370672.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="JN749_004562"
     CDS             672..1358
                     /locus_tag="JN749_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAY7370673.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JN749_004563"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="JN749_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HAY7370674.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="JN749_004564"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="JN749_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HAY7370675.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JN749_004565"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="JN749_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HAY7370676.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4040..4216
                     /locus_tag="JN749_004566"
                     /pseudo
     CDS             4040..4216
                     /locus_tag="JN749_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1201 a          759 c          893 g         1398 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtgggtaa tgactccaac ttactgatag tgttttatgt t
//
LOCUS       DADLTF010000480         3961 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.guided.245, whole genome shotgun sequence.
ACCESSION   DADLTF010000480 DADLTF010000000
VERSION     DADLTF010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.guided.245"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            complement(73..633)
                     /locus_tag="JN749_004567"
     CDS             complement(73..633)
                     /locus_tag="JN749_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAY7370677.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(759..1109)
                     /locus_tag="JN749_004568"
     CDS             complement(759..1109)
                     /locus_tag="JN749_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HAY7370678.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(1312..2325)
                     /gene="intI1"
                     /locus_tag="JN749_004569"
     CDS             complement(1312..2325)
                     /gene="intI1"
                     /locus_tag="JN749_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HAY7370679.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2485..2958
                     /locus_tag="JN749_004570"
     CDS             2485..2958
                     /locus_tag="JN749_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HAY7370680.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3051..3842
                     /gene="aadA1"
                     /locus_tag="JN749_004571"
     CDS             3051..3842
                     /gene="aadA1"
                     /locus_tag="JN749_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HAY7370681.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          918 a         1087 c         1101 g          855 t
ORIGIN      
        1 cgtccttgga gtccggcaac gccagcaggc tttcccgctc ggcggcggac aggatggaac
       61 gacgtggcat atttactgat ccgttctcaa gtattgatac agggtttcgc gactgattcc
      121 gaattcacga gcaagcttgg tcttttgctc gccagcctcg acacgttggc gcagttcggc
      181 aatacgctca gacgacaggg atttcttcct gccacggtaa gccccgcgtt gcttggcgag
      241 cgcaataccc tcgcgctgac gctcgcggat cagggcgcgc tcgaactcgg cgaacgcgcc
      301 catcaccgag agcatcaggt tcgccatcgg agagtcttcg ccagtaaaac tgaggtgttc
      361 cttgacgaat tcgatatgca cgccgcgttg tgtcagcgtt tgcacgatcc ggcgcaaatc
      421 atcgagattg cgcgccaggc gatccatgct atgcaccacc acggtgtcgc cggtgcgggc
      481 gaagcttatc agcgcttcca gttgcggacg cttgacatcc ttgccggatg ccttgtcgct
      541 aaaagcgcga tcaaccttga cgccttccag ttgccgttcc gggttctggt cgaaggtgct
      601 gaccctgata tacccaatgc gctgtccagt catggaattc cctgcaaaat gtcagggaag
      661 actctatgac cttcaacgag atatgtcaat aaattcaaaa ttcaatccta tcctgacgca
      721 atttacacat ggcatctgac atcaggttag ggtatgcctc aacctgacgg cggcgaatca
      781 caagcgtccg gtttgacgct ggtttcggtc gcagtgctgg cccgcgcctg gaagcgctga
      841 tagcactcca gcccgcagaa atgctccacg tactcggccc cttccggcgt gaaggcggca
      901 tcgagcggga tttccttgca gcacacgcag caactggtgc tcggttcatt ggcgttcatg
      961 gtggtgttcc tccattggtt gacgaagccg acgaaggccg ccgccggcat cggcctggcg
     1021 aacaggaaac cctgcaccgt gtcgcaaccc gcctgccgca accacgcaag gcagtcgggt
     1081 gtttccacac cctcggctac cacctccatt gtcgttttca gaagacggct gcactgaacg
     1141 tcagaagccg actgcactat agcagcggag gggttggatc catcaggcaa cgacgggctg
     1201 ctgccggcca tcagcggacg cagggaggac tttccgcaac cggccgttcg atgcggcacc
     1261 gatggccttc gcgcaggggt agtgaatccg ccaggattga cttgcgctgc cctacctctc
     1321 actagtgagg ggcggcagcg catcaagcgg tgagcgcact ccggcaccgc caactttcag
     1381 cacatgcgtg taaatcatcg tcgtagagac gtcggaatgg ccgagcagat cctgcacggt
     1441 tcgaatgtcg taaccgctgc ggagcaaggc cgtcgcgaac gagtggcgga gggtgtgcgg
     1501 tgtggcgggc ttcgtgatgc ctgcttgttc tacggcacgt ttgaaggcgc gctgaaaggt
     1561 ctggtcatac atgtgatggc gacgcacgac accgctccgt ggatcggtcg aatgcgtgtg
     1621 ctgcgcaaaa acccagaacc acggccagga atgcccggcg cgcggatact tccgctcaag
     1681 ggcgtcggga agcgcaacgc cgctgcggcc ctcggcctgg tccttcagcc accatgcccg
     1741 tgcacgcgac agctgctcgc gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg
     1801 atccttggag cccttgccct cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt
     1861 gacccgcagt tgcaaaccct cactgatccg catgcccgtt ccatacagaa gctgggcgaa
     1921 caaacgatgc tcgccttcca gaaaaccgag gatgcgaacc acttcatccg gggtcagcac
     1981 caccggcaag cgccgcgacg gccgaggtct tccgatctcc tgaagccagg gcagatccgt
     2041 gcacagcacc ttgccgtaga agaacagcaa ggccgccaat gcctgacgat gcgtggagac
     2101 cgaaaccttg cgctcgttcg ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa
     2161 ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt ggacataagc
     2221 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     2281 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     2341 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     2401 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     2461 aaagttaacc tctgaggaag aattgtgaaa ctatcactaa tggtagctat atcgaagaat
     2521 ggagttatcg ggaatggccc tgatattcca tggagtgcca aaggtgaaca gctcctgttt
     2581 aaagctatta cctataacca atggctgttg gttggacgca agacttttga atcaatggga
     2641 gcattaccca accgaaagta tgcggtcgta acacgttcaa gttttacatc tgacaatgag
     2701 aacgtagtga tctttccatc aattaaagat gctttaacca acctaaagaa aataacggat
     2761 catgtcattg tttcaggtgg tggggagata tacaaaagcc tgatcgatca agtagataca
     2821 ctacatatat ctacaataga catcgagccg gaaggtgatg tttactttcc tgaaatcccc
     2881 agcaatttta ggccagtttt tacccaagac ttcgcctcta acataaatta tagttaccaa
     2941 atctggcaaa agggttaaca agtggcagca acggattcgc aaacctgtca cgccttttgt
     3001 accaaaagcc gcgccaggtt tgcgatccgc tgtgccaggc gttaaacatc atgagggaag
     3061 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     3121 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     3181 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     3241 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     3301 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     3361 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     3421 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     3481 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     3541 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     3601 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     3661 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3721 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3781 atcagttgga agaatttgtt cactacgtga aaggcgagat caccaaggta gtcggcaaat
     3841 aatgtctaac aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta
     3901 gatgcactaa gcacataatt gcggtgatgc tgccaactta ctgatttagt gtatgatggt
     3961 g
//
LOCUS       DADLTF010000481         1812 bp    DNA     linear   BCT 06-MAR-2021
DEFINITION  TPA_asm: Shigella flexneri strain EG0002
            SAMEA1029850-rid11416023.guided.315, whole genome shotgun sequence.
ACCESSION   DADLTF010000481 DADLTF010000000
VERSION     DADLTF010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA1029850
            Sequence Read Archive: ERR047388
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-JAN-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-JAN-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000942078.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000942078.1
            Genome Coverage       :: 244x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/28/2021 01:08:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,574
            CDSs (total)                      :: 4,517
            Genes (coding)                    :: 4,135
            CDSs (with protein)               :: 4,135
            Genes (RNA)                       :: 57
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 382
            CDSs (without protein)            :: 382
            Pseudo Genes (ambiguous residues) :: 0 of 382
            Pseudo Genes (frameshifted)       :: 194 of 382
            Pseudo Genes (incomplete)         :: 88 of 382
            Pseudo Genes (internal stop)      :: 136 of 382
            Pseudo Genes (multiple problems)  :: 33 of 382
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA1029850-rid11416023.guided.315"
                     /strain="EG0002"
                     /db_xref="taxon:623"
     gene            264..923
                     /locus_tag="JN749_004572"
     CDS             264..923
                     /locus_tag="JN749_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HAY7370682.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="JN749_004573"
     CDS             complement(1124..1501)
                     /locus_tag="JN749_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAY7370683.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..>1812)
                     /locus_tag="JN749_004574"
     CDS             complement(1568..>1812)
                     /locus_tag="JN749_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAY7370684.1"
                     /translation="RYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYL
                     SPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          468 a          431 c          445 g          468 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ct
//
